LOCUS       DAOOWR010000001        20663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_2570_length_20663_cov_3.983550, whole genome shotgun sequence.
ACCESSION   DAOOWR010000001 DAOOWR010000000
VERSION     DAOOWR010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 20663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20663
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2570_length_20663_cov_3.983550"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..854
                     /gene="rnpB"
                     /locus_tag="PLB85_00005"
     ncRNA           444..854
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB85_00005"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1478..2266
                     /locus_tag="PLB85_00010"
     CDS             1478..2266
                     /locus_tag="PLB85_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU71738.1"
                     /translation="MIRTLQTVWHSLILARFFTRSFFIRLFNRNPIKRRWLEIKNTTL
                     TARHFLRAFNVKLKIKNPERLQELKDQNYLLVGNHASFLDIFVLGAVENFVFITSVDM
                     SETPVLGDIIRKAGCLYTDRKKKVSLPDEIKRFTETLRSGFKLVLFPEQSGTDGSHVK
                     KFRRSLFQVAVDAACTVVPVCIRYLRLDGKPVTEKNRSVICWYKGVNFLKYYWNLMAR
                     RLEAELSFLDPVEYDPERNRGELAELVYEQVRDTFNSYDTQLST"
     gene            2280..2474
                     /locus_tag="PLB85_00015"
     CDS             2280..2474
                     /locus_tag="PLB85_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71739.1"
                     /translation="MDKKNDRLQLGPVNFIILGVAAVLLILGYIIMSANEITISPLLL
                     ILAYVVLIPFGLLYKSKPKD"
     gene            2478..3356
                     /locus_tag="PLB85_00020"
     CDS             2478..3356
                     /locus_tag="PLB85_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU71740.1"
                     /translation="MALLTEETAARLKRYQLQARAIVEGFLVGLHKSPYHGFSVEFSD
                     HREYNSGDPVKNIDWKIVAKTERYYVKRFEEETNLRCYLILDHSRSMFYGSGESTKID
                     YACRLAAALAWLMVGQKDATGLITFNERITSMLPPKAYRGYLGPIFRVLAELEPKDST
                     RLLENLHQIASTIRKRSLIILISDLLDDPERVIEGLKHFRSQHHEVLVFHITDLQEDK
                     FSFSQETEFVDSETGEKIVVNPWQIRAQYLSRYSGHINQLKAGCHQYQIEYNPVSTAT
                     PLEDLLLKYLLMRKKG"
     gene            3426..4301
                     /locus_tag="PLB85_00025"
     CDS             3426..4301
                     /locus_tag="PLB85_00025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU71741.1"
                     /translation="MAVRTISANQIKDAVIDAIGEMFCRPTPETAELLRQARDGEKNE
                     IARDMLDAILENHELAVREQLPLCQDTGLLVVFAELGTEIHVEGGTLESLIGEAAHDA
                     WQQFHLRDSIAAYPQRNSFSDKGMEVQGKRSGNPRLPVVLHLEQVPGDQLTLHLALKG
                     GGAENCGTLKMFLPGAKLEEIEDFVVRTVVDAGGKPCPPVFVGVGIGGDFESCAILAK
                     RALMLEEPKDADIEQMEGRILQRINLMGKGVMGFAGNTTALKVSILTRPRHIASLPVA
                     VNIDCHAHRSTSRVI"
     gene            4341..4844
                     /locus_tag="PLB85_00030"
     CDS             4341..4844
                     /locus_tag="PLB85_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOU71742.1"
                     /translation="MKTHRLSLPIPSEAISRLEVGDKILLSGEVYTARDQAHIRMLEI
                     IGRGESLPFDLEKTAIFYCGPAPTPPGRVSGSIGPTTSTRMDPYTIALLEKGLKVMIG
                     KGERSSEVESAIRQHHAIYLICVGGISALLAQCVVSRETFLWPELGAEAVYRLVVKNF
                     PCYVAIV"
     gene            complement(4891..>5719)
                     /locus_tag="PLB85_00035"
     CDS             complement(4891..>5719)
                     /locus_tag="PLB85_00035"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HOU71743.1"
                     /translation="YWVNLTSGSRIMALAAFEHFRKLQSQVFYLPPYRNVFMQLWPDH
                     IEHHLPIKFKLCLKSCLEAHGVKITRISSNQHSLEQLEAMYAIITQNSNSRFMNLLNR
                     LGSEKRSANEENKLARLARKFSQALGISEYEVETPAWLSFIKGAWFEEYMAYWLDRIV
                     DEVYYGVVIEMDGVQNELDCVFMHENRLHFVELKASANIGDLNEFLYKLDSLGKDFGL
                     LPRCFLGIADPDVEHGLRHNRQYLRRAKVMGINILTYSQLKPENIEKTLREVLFSSG"
     assembly_gap    5720..5729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5730..5964)
                     /locus_tag="PLB85_00040"
     CDS             complement(<5730..5964)
                     /locus_tag="PLB85_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71744.1"
                     /translation="MSRYLVSLIGELVIPNLLLIKTLNAVNRHVFLCPRGGEYRAQVG
                     RLCAVSDIDEPLLLEVEEFSLEDTDRVLKNHLNG"
     gene            complement(6116..7657)
                     /locus_tag="PLB85_00045"
     CDS             complement(6116..7657)
                     /locus_tag="PLB85_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71745.1"
                     /translation="MKPGLVAFLLLSLSCLAAQIPDELTELDYTARAETAWEQLHQEL
                     LCQPVSLDARATLAPNNSQFQTSASCHISQAELLLHYNYRAKDALSAGSFRLKASSPR
                     WDLALGSYRFRFGRGLVSGSNSRAQQDSLFSLLEPLSPRNFSPQGAIVIHRYGFFRGA
                     LFGSVQDREAKTDAEGRIQTLFKTRTGRLSTTRENIIGMAAGVAFPSVQASGLLYWSQ
                     YDRDFAESGIDPALWAGSLYAAFSHKNLRLDAEAFLAEGIPSALLTCSYKLNQFSQSL
                     SYAHNGFQDRLPYALSPGVLSSAALRDEFNGDLSFGLPLHTNLKLRFTLNSGSGFSGD
                     ALSRFTGSAGYSHKGNHLKLLFHNYDREIISLVDSNYVSSEPRNWRFQLNGQFRFLNN
                     FYQRLVFSYTLQDKTDASRNTYRVRYGFFWSQGAWKLGLDYLAWQSSEEIWEVDELDP
                     YNLVAEMAEDKLLVADLAWKSGPWRLGLRGRLSFLDQDSCRLWLRCGWSWPGRSADSS
                     NPPKK"
     gene            complement(7654..8403)
                     /locus_tag="PLB85_00050"
     CDS             complement(7654..8403)
                     /locus_tag="PLB85_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71746.1"
                     /translation="MRVLILACICLLAFPLGALILHNTASANALAGITLLSESVADFQ
                     LSPALPFTGIAASGHLPFSAPEAGTFSLSTAHKLSFLVLNAGVSHSGDADYSLQDFRL
                     GLALNYEGLSAGYAQHLTLESAATGSYQSGSGDAAVSYRTPGYGYEARLLRIGKEDTE
                     LHLSVSTKIVTGIQAASSYVYNPGGPDGYRLATSIEVTDNLLLQSSWQSSPNRFGAGL
                     KFQLKGWDLMYAIRTHPDLRLSHSLDLGYTW"
     gene            complement(8421..9524)
                     /gene="ugpC"
                     /locus_tag="PLB85_00055"
     CDS             complement(8421..9524)
                     /gene="ugpC"
                     /locus_tag="PLB85_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOU71747.1"
                     /translation="MAKIVVKDLNKYYDNSVHAVIDANFEAEDKEFIVLVGPSGCGKS
                     TVLRMIAGLEEVSSGEIWIGDTLVNDVPPKDRDIAMVFQNYALYPHMSVYDNMAFALK
                     LRGEDKESIRKKVQHAAELLGIQDMLKRKPAQLSGGQRQRVALGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRAEIVKLQRQLANTMIYVTHDQVEAMTMADRIVVMKDGVIQQIDT
                     PLKIYNDPANIFVAGFIGSPAINMVSGSVQTKGGELVFDSGDFQLEIFPAHREALQNY
                     TGKPVIMGIRPEDIYDARYDSMADFPRKLATTCDIVEPLGNEYHVVLRTAKNEFTARF
                     DPKELPVPGAPLEVSLNLAKAHYFDPETELSLV"
     gene            complement(9582..11225)
                     /locus_tag="PLB85_00060"
     CDS             complement(9582..11225)
                     /locus_tag="PLB85_00060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOU71748.1"
                     /translation="MTDGTDAKTPIPVSWRRKKGLREMLALLLDSSGPQREYLINIIL
                     AQGSGKPGHEPSQRETRFRCANIIEQLVGLLRSEAVQSKSQLLPFFKYLDSFPLDPLQ
                     ETQTALSLIFHGGPSKEDKKLFYRLELFWLLTLMGDLDSAGPLRKELEPQISLEKPRL
                     YIIWLLSLARILQKRGKRISFSTLWLNIICEYYRLDGAATALYLILRWVRMINWGRDT
                     ALRKKVLHRFGARLRQRKDLLSATLLYELFDQEHRHANPSEKMLYTKLLFRHSSSLLS
                     VQQLQYLHFFAGNYLSAVKSSFPQSIREFQHSNYYLHKSWSHLRNLSLFLRANLRDDQ
                     YAQSMSYLQQRVVDLSSQISLQNNAYVETLHENYSTIKKLLRQVEELSITDNLTGLKN
                     RRYLTNNLYHAFQLAARHKVPVCLAILDIDYFKSVNDQLGHLAGDQVLKSLARLLQSS
                     FRKSDAVIRYGGEEFLLVLFDVDPERMSEMMEELRRKVKNQRFSWQGNPIRISVSIGW
                     ASCPACLKQSELEEHIARIDAALYTAKSTGRDLVVHATD"
     gene            complement(11281..11625)
                     /locus_tag="PLB85_00065"
     CDS             complement(11281..11625)
                     /locus_tag="PLB85_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU71749.1"
                     /translation="MKKQQPKHKALFNFIYIALFLFILSWILFWGSNSFYQKWRLQQS
                     AARKEKLVEALEAQNDSLKQENQRLKTDPEERKRAIRATLGIYAEDETAYIFKPAQED
                     SMATKAGKNGEK"
     gene            complement(11622..12686)
                     /locus_tag="PLB85_00070"
     CDS             complement(11622..12686)
                     /locus_tag="PLB85_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU71750.1"
                     /translation="MADFLGHANRIRIKRSKRVLLKPNLLGAFPPERAVTTHPVLLEA
                     LIRHFLELGKEVWIGDSPGGTADVEQVWQACGLKDLANRYPVKLVNLSTSGYRELKWN
                     GIPVKISEVFWQCGIVINIGKYKTHGLVGFTGALKNLYGLVPGLIKTDYHSRYPNSVD
                     FSHLLLALYGLTRARLSYSFIDGILGMDGIGPSGGRVRKFGLLLGSRSVSSLDFTAAR
                     MMGFRLDDVPYLSEALQMDGVLPSRVQVPTSFRNFTLPDVDIRAVKYSYRVLRRVPRF
                     ARHLVQRYYSKRPFISDRCKKCGICVQSCPVQAISPPGRFTLPKIDPRKCIKCMCCHE
                     MCLHGAVDIHKTFLTRMIEK"
     gene            complement(12746..13945)
                     /locus_tag="PLB85_00075"
     CDS             complement(12746..13945)
                     /locus_tag="PLB85_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71751.1"
                     /translation="MRRNIICLLMLGLLGTALSAQSPDVLNFPQPQYVNPIFDSFTRN
                     YMSTSAMGRGYTGLAIPGGVDNVLKNPAGYHPDKASLHLEMLVKPPVESDIYAMADTS
                     VYYRMTKENMLSPVPVGIVGGGGTIGPNFTYGLLYSMPKSIKVDNFGVEVNMGYGLIM
                     RYPSFFLNQLTANAAWHKDNFHVGLNLHNQIYHLGDVTFLRSFERIISDKYVLRPQLG
                     LLYSGESLSAGLSMVPQQNVNWDLKFAQYDTRLPLNLALGVAYNKNNAVFTAEVDYEQ
                     CSAISDAFKDRYTFHAGMEKTVRRYTYRAGYIYHPQVWEGDFLIPDACSDTISIWWAA
                     VLPQGRVGKNNQHILTLGTSWNHPDVRVNLSGLIDVAGQAPIAQVSLSADLFFSAFKR
                     KNFLYFD"
     gene            complement(13960..15216)
                     /locus_tag="PLB85_00080"
     CDS             complement(13960..15216)
                     /locus_tag="PLB85_00080"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU71752.1"
                     /translation="MSSLTVLGCMWGDEGKAKIVDFLGANADVVARFQGGANAGHTIV
                     LEGNKYVFHTVPSGILYPKTKCVIGAGTVIDPYGLRGEMRALMKQGIDFGGRMFIDVR
                     AGLVLPLHKELDGSTEEELGASKIGTTGKGIGPAYSDQAARCGLRFGDLGHPDWLKQR
                     LASLYAYHDLKPRDLKCELKELAEVWEFLKPFAAQADELLRNCYLAGESMLFEGAQGT
                     LLDLTYGTYPYVTSSNTTTGGASIGIGFPPRWLDKTIGVYKAYVTRVGEGPFPTEIFD
                     ETAEQIRAVGNEFGATTGRPRRIGWFDAVAAKYTASLNGLDALALTLLDVLSGLPELK
                     ICTGYWLDGKKLPGYTPHPLILERLEPEYLSLPGWEADLGKIRSLHRLPKTAKEYLEA
                     VQDLVEVPIELVSVGKDRTQTIVVKR"
     gene            15718..16338
                     /locus_tag="PLB85_00085"
     CDS             15718..16338
                     /locus_tag="PLB85_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71753.1"
                     /translation="MKRMYLLLLPLLALLFLAACESGGNFRVINRTSYPVYVTLDDEA
                     EFAIPAGTEHTFEVATANQHIFNPDVSVEVPVRLVGETFQIYDEGNEVFTDTTRVVIH
                     AGKTTSVFVDPNRASFKVVNNSSQYIIQAKLFKHNFVGVVASNDTGPIAPGRFVYLPV
                     EYATSSNNFYYYATVQMEDGATYNYGNETNILAVDQQFLITLTDPE"
     gene            16348..19164
                     /locus_tag="PLB85_00090"
     CDS             16348..19164
                     /locus_tag="PLB85_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU71754.1"
                     /translation="MNPNVKLFATLILTALLTGLFAQPAEMMQIPLQQDPAVLSGQLA
                     NGLSYYIMRNPKPANLAELRLYVDVGSVNEDADQLGLAHFTEHMAFNGTKNFAKNEVV
                     EYLSSIGMGYYNGLNAMTSYDYTVYQLKIPTDDSAKLNQGLLILSDMAHQVSFEASEI
                     ERERGVIIEEWRLGQDAGSRVRDAQNAVFLAGSRYAERSPIGKREVLESFEHDTIKRF
                     YRDWYRPDLQSVVIVGDFEPQDMLALVERYFGVIPKREEVRPREDFSVPENLAPQAIV
                     TTDPEYPNNLLQVMWKKDVQRMSNLGEYYDNLKTSLFYTMLNLRLEEKSKTADPPYSF
                     AAATEYPLLRTMSAAMMFAMYTQGKSEEALRTVLTEAERVQRYGFLPSEFERAKIDIL
                     RGAEQDVAEQGTRESGRIAWEIVDAVSNQNVFTSAEFRQMLTQELIQAVTLEEVNALV
                     ENLIQDKNMFIGLAGPQKEGLTYPTKERLLEIASGVNSLEIEPYEDKTVNEPLLEVIP
                     EPVAIKKESFDAKSGVNIWTLANGVKVYAKKTDFKNDEVLLQAISPGGFSRYDTTDVP
                     AARLVSGYISDAGFGKFDAISLGKATAGKVARVNLSVDTYQESFDASCSPKDMELMFQ
                     MLHQYAVNPRFDRADFASYLQRTKTFYQNRNLDPMNVFMDRVQKELYQNHPYAKNLAD
                     ADFDTIDMDAVERIFRDRFADFSDFSFVIVGNFDPELLKTYCRTYLGNLPCGGRLEKP
                     KDVNIRLRKGLQSIWFNMGSTDRAFASTLLNGKYNSSTKNKVMLDALEFVLNEKLREN
                     IREERSGVYFIQAWQSTTSYPDSRYTVGTMMGCAPARLKELNDAIFATLDSLRAGHID
                     KHYLVAANATLRQQFDENIKSNRFWIYQLKSDIWQGRKLASFLDEPAFFDQIDLKNIA
                     KAAKKYLRFGKNQLSVFMLPQVQE"
     gene            19297..>20663
                     /locus_tag="PLB85_00095"
     CDS             19297..>20663
                     /locus_tag="PLB85_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU71755.1"
                     /translation="MADKIKATMDGNTAAAHVAYAFAEVAAIFPITPSSTMGELADAW
                     AADGRKNINGTTVDVIEMQSEAGAAGAVHGALSAGAVTTTFTASQGLMLMLPNMHKIA
                     GEMLPTVFYVTARSLAAQSLSIFGDHSDVMSARNTGWALISANSVQEVMDLGLVAQLA
                     TMKTSIPFLVFFDGFRTSHELQKIEVIDYETMAELNDPALVEAFRLRALNPERPKVKV
                     GQQSPDVYFQGRETTNRHYMEAPATIAATMDEVAAKIGRAYKPFDYVGHPEAEHVIVA
                     MGSGCEAIEETINYLNAKRGMRLGLVKVRVYRPFSVEYFLSVLPASVKNVAVLDRTKE
                     PGSIGEPLYLDVVSALKDRPGLFIIGGRYGLSSKDFTPTMILAVYKHLMAKGFHGFTV
                     GIEDDVTKLSLPLDEYIDPSPEGTINCKFWGLGSDGTVGANKNSIKIIGDNTDLYVQG
                     YFQYDS"
BASE COUNT         4763 a         5597 c         5766 g         4527 t
ORIGIN      
        1 cccagccccg cgtccgaatc tacaaccttc gcggtgccag gattaggatt tagtagtctc
       61 aacggatgac gccaactgcg tttgggacgg caacctggaa tccgggcagc ccgctcctgc
      121 cggaatctat ctgctgcgcg cggaagccgg caagaactca gaactgcggc gggtggttaa
      181 aacacgctga aaagcctcaa tatcccgggg ccggccttac aaaatacatc actaacttat
      241 ctattttcaa taccttcaaa cattaccatc cataacctca cttcaaataa acggggttca
      301 cttccaaacc gagtgggtag cccgatgggc tcgatcaaac agtttaccca ctcggtttga
      361 aagtgagcca ttaatagtta ggtagattca gcgttttttc ccttgacgat atagctgtct
      421 tgctaaaatt gcttccagac ccatgggaaa accagatgtc cgctgcatcc ttagcgggtg
      481 cagaggaaag tccggacacc acagggcggg atacttccta acgggaagcc gcagcaatgc
      541 ggagcagctc cacagaaaca aaccgcccgg cgcgccctcc ttttattggt tgccgttgag
      601 agaccgcggc ttttttgtgc cgggcaaggg tgaaatggtg gtgtaagaga ccaccgtaag
      661 ccttggtgac agggcttgac gggaatgcct tatccggtgc aatgccaaat agggaagcca
      721 ggaagcgcgg cccgcgcggt tacggcttcc gggtaggcag cttgaaccct ttcgtgagaa
      781 agggtccaga ggaatggaca tcagcccgcg cgtgagcgct ggccacagaa tccggcttac
      841 cgttctcccg tgggtttttt gcacctttgg atgtgtaaaa gcgcgcaaaa caaaccaggc
      901 tccattactg taactgccag ccaaacatac ggttagatat atccatgttt ctcggcctgc
      961 tcatcttcgt tgattcctcc tttgcccttt cccatacaca tcctgaccgc cactcgctta
     1021 gttcccgcag cccgaacggg aactaagcgg gaagtgaatg ggatgtgaat aagatagggt
     1081 aaatgaggag cagatcaggc aaatggggca tcacacagat tccgccaagt accggcactc
     1141 attgaagcgc cgacgcgatg aaaatgttgg cggggaggct ggcgtagagc gaggaacgag
     1201 catcgacgac agcaacattt cctgccgtcc atgcttactg cgttcgctgg acgtcagccc
     1261 tccgggctca attaaccgga ttacccactc gatttgaaag agagcctaat caaagtaccc
     1321 ttacccacca agttaaaaaa gaacctcaaa tcacgcctat tacagcgggc tgggcaaatt
     1381 ttcttgacag attttcgttc tgccggcatt ttgcctatca gaaggcggaa tctgcggaca
     1441 agcctccaaa aaacacttgg gagtaaccgt gaagtggatg atcaggactc tgcaaacagt
     1501 ctggcacagc cttatcctgg ccaggttctt cacgcggtct ttttttatcc ggctttttaa
     1561 ccggaaccct ataaaacgcc gctggctgga gattaagaac accaccctga ccgccaggca
     1621 tttcctgcgc gcgttcaatg tgaaactgaa aataaaaaac cctgaacgcc tgcaggaact
     1681 gaaggaccag aattacctgt tggtgggaaa tcacgcctct tttctggaca tctttgtgct
     1741 gggcgccgtg gaaaacttcg tgttcatcac ctcggtggac atgagcgaaa ccccggtgct
     1801 gggagacata atccgcaagg ccggctgcct ttataccgac aggaagaaga aggtgagcct
     1861 gcctgatgaa ataaagcgct tcaccgaaac gctgcgttcc ggcttcaaac tggtgctgtt
     1921 tcccgagcaa agcggcacgg acggctccca cgttaagaaa ttccgccgct cccttttcca
     1981 ggtggccgtg gacgccgcct gcaccgtggt gccggtctgc atccgctatc tgcgtttgga
     2041 cggcaaaccg gtcacggaaa agaaccgttc ggtgatttgc tggtacaaag gcgtgaattt
     2101 tttgaagtat tactggaacc tgatggcgcg ccggctggag gctgaactca gctttctgga
     2161 tcccgtggaa tacgatccgg agcgcaaccg cggtgaactg gcagagttgg tttacgagca
     2221 ggtccgcgac actttcaaca gttacgacac ccaactttca acatagaaga ggagatatca
     2281 tggacaagaa aaacgaccgc ctgcaactgg gccccgtgaa tttcatcatc ctgggagtgg
     2341 cggcggtgct actcattctg ggctacatta tcatgtccgc caacgagatc accatctcgc
     2401 cgttgctgct gatactagcc tatgtggtgc tgattccttt tgggctgctc tataaaagca
     2461 aacccaagga ttaaggcttg gccctgctca cagaggagac cgcggcccgg ctgaaacgct
     2521 atcagctaca ggcccgggcg atcgtggagg gttttttggt gggtctgcac aaatcccctt
     2581 accacggctt cagcgtggaa ttttccgacc accgagaata caacagcggc gatcccgtta
     2641 aaaacatcga ctggaagatc gtggccaaaa ccgaacgcta ttacgttaag cgcttcgagg
     2701 aagagacaaa ccttcgttgc tacctcattt tggaccactc ccgctccatg ttctacggct
     2761 ccggcgaaag caccaaaatt gactacgcct gccgtctagc cgccgctctg gcctggctga
     2821 tggtggggca gaaagacgcc accggattga taaccttcaa cgagcggata acctccatgc
     2881 tgccgcccaa ggcctaccga ggctatctgg gcccaatttt ccgcgtgctg gcggaactgg
     2941 agcccaagga ttccacccgc ctgctggaaa acctccacca aatcgcttcc accatccgca
     3001 aacgcagcct catcatcctc atctcagacc ttttggacga tcccgaacgg gtgatagagg
     3061 ggctcaaaca tttccgctcc caacaccatg aagtgctggt ctttcacatc accgacctcc
     3121 aagaagacaa attcagtttc agccaggaaa cggaattcgt ggacagcgag accggcgaaa
     3181 agatcgtggt caacccctgg caaatcaggg cccaatatct ttcccgctac tccgggcata
     3241 tcaaccagct caaggccggc tgccaccaat accagataga atacaacccc gtgagcaccg
     3301 ccacgccgtt ggaggacttg ctcttaaaat acctcctgat gcgcaaaaaa ggatgagatt
     3361 ggagtcgtat gtgcgctaaa cggataaaac ttcaaaaaca cgcctttccg ggcgtttaaa
     3421 cggctatggc tgtaagaacc atttccgcca accagatcaa agatgcggta atagacgcca
     3481 tcggtgagat gttctgccgt cctaccccgg aaacagcgga actgttgcgc caagccagag
     3541 acggagaaaa aaacgaaatc gcgcgtgaca tgctggacgc catcctggaa aatcacgaac
     3601 tagctgtgag ggaacaactt ccgctttgcc aggatacagg tctgctggtc gtgttcgcgg
     3661 aactgggaac ggaaatacac gtcgagggtg gaaccctgga atctcttatc ggagaagcgg
     3721 cacatgatgc ctggcaacaa ttccatctga gggattcgat tgccgcttat ccccaaagga
     3781 actcattctc agacaaaggg atggaagttc agggaaagag gtctggtaac cccaggctac
     3841 ctgtggtttt gcacctcgaa caggtaccgg gtgaccagct aaccctgcat ctggccctca
     3901 aaggcggagg cgcggaaaac tgcggcacgc taaagatgtt cctgcccggg gccaagctgg
     3961 aagaaataga ggattttgtg gtgcggactg tggttgatgc tgggggaaaa ccctgtccgc
     4021 cggtttttgt gggagtgggc atcggtggag atttcgaaag ctgcgccatc ctggccaagc
     4081 gggctttgat gctggaagag cccaaggatg cggatattga gcaaatggaa ggaagaatcc
     4141 tccaaagaat caatctaatg ggtaaagggg taatgggttt tgccggaaat accaccgctt
     4201 tgaaagtttc catcctcacc cgtccccggc acatcgcctc cttgcccgtg gctgtgaaca
     4261 tcgactgcca cgcccaccgg agtacttcca gggttatctg atgtttcacg tgaaacatgg
     4321 ttactcagca tgatttaaat atgaaaacac atagacttag tctcccgatt ccatccgaag
     4381 caatttccag attggaagtt ggcgacaaga tcctgctcag cggtgaggtt tatacagctc
     4441 gagatcaggc gcatatccgg atgttggaga taatcggcag gggagaaagc ctgccctttg
     4501 acctggagaa aacggccatc ttctattgcg ggccggctcc aacaccacca ggcagggtga
     4561 gcggttcaat cggtcccaca acctccacca gaatggaccc ttacaccata gccttgctcg
     4621 aaaagggcct gaaggttatg atcggcaaag gtgagcgttc ttctgaagtg gaatcagcta
     4681 tcaggcaaca ccatgcgatc tatctgattt gcgtgggtgg aatctctgcg cttctggccc
     4741 aatgtgtggt ttcacgtgaa acatttctct ggccggagct aggagctgaa gctgtctatc
     4801 gtcttgtggt aaaaaacttt ccctgttacg tggctatcgt ctgataatct tttcaagaca
     4861 agcgtctgct cctccactcg cgtccatccc tcatccacta ctgaataaaa cctcccgcag
     4921 ggtcttttct atgttttcgg gttttaactg gctgtatgtg aggatattta ttcccatcac
     4981 cttggcccgg cgcaggtact gccgattgtg ccgcaagcca tgttccacgt ctggatcggc
     5041 gatgcccagg aaacaacggg gcagcaaacc gaagtctttg ccaagggaat ccagtttgta
     5101 aagaaactca ttgaggtcgc cgatgttggc tgaggctttc aattccacaa aatggagtcg
     5161 gttctcatgc atgaaaacgc agtccaattc gttttgcacc ccgtccattt ctatcaccac
     5221 gccgtaatag acctcatcca cgatcctgtc cagccagtaa gccatgtatt cctcgaacca
     5281 ggcgcccttt atgaaagata gccaggcagg ggtttcaact tcgtattcgg aaattcccaa
     5341 agcctggctg aattttcttg ccagtcgggc caatttgttc tcttcattgg cactgcgttt
     5401 ttctgagccc aggcggttca gcaagttcat gaaccgggaa ttggagtttt gggtgatgat
     5461 ggcgtacatc gcctccaact gctccaggct gtgttgatta gatgatatgc gtgttatctt
     5521 taccccatgg gcttccagac aacttttcag acacagtttg aatttgatgg gaagatgatg
     5581 ttcaatgtga tcgggccaga gctgcataaa aacattgcga tacggcggca ggtagaatac
     5641 ttgggactgc agtttccgga aatgctcaaa agctgccagg gccatgatcc gggaaccgga
     5701 ggtgagattg acccaatatn nnnnnnnnng accgttaaga tggttcttca gaactctatc
     5761 cgtgtcttcc aaactgaatt cctcgacctc cagcagtagc ggttcatcaa tatcgctcac
     5821 ggcgcaaagc cgcccaactt gtgcgcggta ttctccaccc cggggacaga ggaaaacatg
     5881 cctgttcaca gcgtttaacg tcttgatcaa caggagattg ggaatcacca gttctccgat
     5941 cagggacacc aaataacggg acatcaatca cctcggatag cttttgcgaa caacatctca
     6001 tccatcgcta aggcggtcaa ccctttttcc tccagcggag ttaccacttg atacttccga
     6061 cgccagaggg ttgttcataa aggtatttta ttaagattgc cgcaactcat gaatttcact
     6121 tttttggggg attacttgaa tccgcagatc ggcctggcca gctccaacca catcgcagcc
     6181 aaaggcggca ggaatcctga tccagaaagg ataatcgccc ccgcaagcca agtcgccagg
     6241 gtccgctttt ccaggccaga tcggctacca gcagcttgtc ctccgccatt tccgccacca
     6301 gattgtatgg gtccaactca tccacttccc agatttcctc ggagctttgc caggcaagat
     6361 aatccaaacc cagcttccaa gccccttgtg accagaaaaa gccgtagcgg acccggtagg
     6421 tgttgcggct ggcatcggtt ttatcctgca acgtgtagct gaaaaccagc cgttggtaaa
     6481 agttgttcag aaagcggaat tggccgttca gttgaaagcg ccagttacgc ggttcggagg
     6541 acacatagtt ggaatccaca aggctgatga tctcgcggtc gtagttgtga aaaagcagtt
     6601 tcagatggtt gcccttgtgg ctgtaacctg cagagccagt gaagcgggaa agagcatcgc
     6661 cgctgaagcc ggagccggaa ttcagggtga agcggagttt caggtttgtg tgcaaaggca
     6721 ggccgaagct caaatctccg ttgaattcat ctcttaaagc agccgaagaa agcaccccgg
     6781 gagaaagggc gtaaggaagc cgatcctgga agccgttatg ggcatagctg aggctttggg
     6841 agaattgatt gagtttatag ctacaggtga gcaaagcgct gggaattccc tctgccaaaa
     6901 acgcttcggc atccaggcgc aggtttttgt ggctgaaggc tgcgtatagg cttcccgccc
     6961 aaagggcagg gtcgattccg gattccgcga aatcccggtc gtattggctc cagtagagca
     7021 atccgctggc ctgaacgctg ggaaatgcca ctcctgctgc cattccgata atgttttccc
     7081 gggttgtaga gagcctgccg gttctggttt tgaacagggt ttgaatcctt ccttcagcat
     7141 ccgttttggc ttccctgtcc tgaactgaac cgaacagggc ccctcggaaa aacccgtaac
     7201 ggtgtatgac aatggcgccc tggggactga agttcctggg ggaaaggggt tcgagcaggc
     7261 tgaacaggct gtcctgttgc gcccgggagt tggaaccgct cacgaggccc cgtccgaagc
     7321 ggaatctata actgccgagg gccagatccc aacggggcga ggaggccttg aggcggaagg
     7381 aaccagcact cagggcgtct ttggcgcggt aattgtaatg cagcaacaac tccgcttggg
     7441 agatgtggca ggaggcgctg gtctggaact gggaattgtt tggcgcaagg gtggcccggg
     7501 catccaagga aacaggctgg cagagcagtt cctgatggag ctgttcccag gctgtttctg
     7561 cccgggcggt gtagtccagc tctgtcagtt catcggggat ttgggccgcg aggcaggaaa
     7621 gggaaaggag caggaaggca acaaggccgg gtttcaccag gtgtagccaa gatcgaggct
     7681 gtgagagagg cgaaggtctg gatgggttct gatggcgtac atcaggtccc agccttttag
     7741 ttggaacttg agaccggcgc cgaagcgatt gggtgagctt tgccaggagc tttgcagcaa
     7801 aaggttgtcc gtcacctcga tggaggtggc gaggcggtaa ccgtcaggtc cgccagggtt
     7861 gtaaacatag ctggaagcgg cctgaatgcc ggtcacgatc tttgtgctca cgctgagatg
     7921 gagttctgtg tcctccttgc cgatcctcaa caggcgggct tcatagccgt agcctggggt
     7981 gcggtatgaa acggctgcgt cgccgctccc gctttggtag ctgcctgtgg ccgcgctttc
     8041 cagagtcaga tgctgggcat agccagcgct caagccttca taattcaggg ccagccccag
     8101 ccggaaatcc tgcaagctgt aatccgcatc tccactgtgg gatacacctg cattgagcac
     8161 cagaaatgac agtttgtgag ctgtggaaag ggagaaagtg ccggcttccg gggcgctgaa
     8221 aggcagatgg cctgaagcgg ctatcccggt gaaaggcagg gcgggcgaaa gctggaaatc
     8281 ggccacggat tcagaaagca aagttatgcc tgccaaagca ttggcggagg cagtgttatg
     8341 gagaatgagc gcgcccagag ggaaagccaa aaggcagatg caggccagga tcaagacgcg
     8401 catggaaacc caggaggagc tcagaccagg ctaagttccg tttcgggatc gaagtagtga
     8461 gccttggcca ggttcaggct cacctccagc ggggcacccg gaacaggcag ttccttgggg
     8521 tcaaagcggg ccgtgaactc gtttttggcg gtgcgcaaca ccacgtggta ttcgttgcca
     8581 aggggctcaa cgatgtcgca ggtggtggca agcttgcggg gaaaatcggc catggaatcg
     8641 tatctggcgt cgtagatatc ttcggggcgg atgcccatga tgacgggttt tccggtataa
     8701 ttctgcagag cttcgcgatg ggcgggaaag atttccaact ggaaatcgcc gctgtcgaag
     8761 accagttcgc cgcctttggt ttgcacgctg ccagacacca tattgatggc gggggagcca
     8821 atgaatcctg ccacaaagat gttggcgggg tcgttgtaga ttttaagtgg ggtgtctatc
     8881 tgctggatca cgccatcttt catcaccaca atgcggtcgg ccatggtcat ggcttcaacc
     8941 tggtcgtgtg ttacgtagat catagtattg gcaagctggc gctgaagctt aacaatctcg
     9001 gcgcgcatgg ccacgcggag cttggcatcc agatttgaaa ggggttcatc gaagagaaag
     9061 accttggggt tgcgcacgat ggcccggccc agggcaacgc gctggcgctg accgccagaa
     9121 agctgagccg gcttgcgttt cagcatgtcc tgaatgccaa ggagttcggc ggcgtgctgc
     9181 acttttttgc ggatgctttc cttgtcctcg ccgcgcagtt tgagggcgaa ggccatgttg
     9241 tcatatacgc tcatgtgggg atagagggcg taattttgaa agaccatggc gatgtcgcgg
     9301 tcttttgggg gaacgtcgtt gaccagggtg tccccgatcc aaatctcacc gctggaaacc
     9361 tcttccaaac cggcgatcat tctcagcacg gtgcttttcc cgcagccgga ggggcccacg
     9421 agaactatga attccttgtc ctcagcctcg aaattggcgt ctataacggc gtggacgcta
     9481 ttgtcataat acttgttgag gtctttaaca acgattttgg ccatctgaat actcctttga
     9541 gcaaacaaaa aattcggtgg gcaaagctgt caagcaggaa atcagtccgt ggcatgcaca
     9601 accaggtcgc ggccggtgct tttggcggtg tagagggctg catcgatgcg ggcgatgtgt
     9661 tcctccagct cgctctgttt gaggcaagcg gggcaacttg cccagccgat gctaacgctt
     9721 atgcggatgg ggttgccctg ccagctaaaa cgttggtttt tcaccttgcg gcgaagctcc
     9781 tccatcattt cagacatgcg ttccgggtcc acgtcgaaaa gtacaagcag gaattcctca
     9841 ccgccatagc ggataacggc atcgctcttg cggaatgagg actgcagcag cctggccagg
     9901 cttttcagca cctggtcgcc ggcaaggtga cccagttggt cattcacgct cttgaaataa
     9961 tcgatgtcga ggatggccag gcagacaggc actttatgtc gggctgccaa ttggaaggcg
    10021 tggtagaggt tgttggtcag gtagcggcgg tttttgaggc cagtgaggtt gtccgttatg
    10081 gagagttcct ccacttggcg aagcagtttt ttgatggtgc tgtagttttc gtgcagggtt
    10141 tccacgtagg cattgttctg caggctgatc tggctgctca agtccaccac tcgctgctgc
    10201 aggtagctca tggattgggc atattggtcg tcgcggaggt tggcacgcag aaaaagggag
    10261 agatttcgca ggtgggacca gcttttgtgc agatagtagt tcgaatgttg aaattcccgg
    10321 atcgactggg gaaagcttga tttcacagcc gacaggtagt tgccggcaaa aaagtggaga
    10381 tactgaagct gctgcacgct gagcagggaa gaggagtggc ggaacagaag cttggtgtag
    10441 agcatctttt cgctgggatt ggcgtggcgg tgttcctggt cgaagagctc atacaaaagg
    10501 gtggcggaga ggaggtcctt ccgctgacgg agccgggcgc cgaacctgtg caaaaccttc
    10561 ttgcggaggg cggtgtcgcg tccccagttg atcatcctca cccatctgag gatgaggtag
    10621 agcgcggtgg cggcaccgtc caggcggtag tattcacaaa tgatgttcag ccacagggtt
    10681 gagaagctga tgcgtttccc ccgcttttgg agaatgcggg ccaaagaaag cagccagatg
    10741 atgtagagac ggggcttttc cagggagatt tgtggttcca gctctttgcg caggggtccg
    10801 gcgctgtcca gatcgcccat cagggtgagc aaccaaaaga gttccaggcg gtaaaagagc
    10861 tttttatcct ctttgctggg acccccgtgg aagataaggc tcagagcggt ctgggtttcc
    10921 tggagagggt caaggggaaa gctgtccaga tacttgaaga aaggcagcag ctgactttta
    10981 ctttgcaccg cctcggaacg cagcagcccc accagttgct caatgatgtt ggcacagcgg
    11041 aagcgggttt cgcgctggga cggttcatgg ccgggcttgc ctgaaccctg ggccaggatg
    11101 atgttgatca gatactcgcg ctgcggccca ctggaatcca gcagcagagc cagcatttcg
    11161 cgcagacctt ttttgcggcg ccagctcacc ggtatcgggg ttttggcatc agtcccgtcg
    11221 gtcattcagg ctccaaatgg caatgcgggc ccctctgccc ggcaaaatcc aggcggggat
    11281 tcatttttct ccgttcttac cggcctttgt ggccatgctg tcttcctggg ccggcttgaa
    11341 aatataggcg gtctcatcct cggcgtagat gcccaaagtg gcacggatgg cgcgtttgcg
    11401 ttcttccgga tcggtcttca gccgctggtt ttcctgcttg aggctgtcgt tctgagcctc
    11461 cagcgcttcc accagtttct ctttcctggc ggcgctttgc tgcaagcgcc atttctggta
    11521 aaaactgttg ctgccccaga acagtatcca actcaggatg aacaggaaca gggcgatgta
    11581 gataaagttg aaaagggctt tatgcttggg ctgttgtttt ttcatttttc gatcatcctg
    11641 gtgagaaagg ttttatggat atccaccgcg ccgtggagac acatctcgtg gcagcacatg
    11701 cacttgatgc acttgcgggg atcgattttg ggcagggtga atctgccggg aggggaaatc
    11761 gcctgcacgg gacagctttg cacgcagata ccgcattttt tgcagcggtc gctgataaaa
    11821 ggccgcttgc tgtaataacg ctgcaccaga tgccgggcga aacggggcac gcggcgcagg
    11881 acgcggtagc tgtatttcac ggcccggatg tccacatcgg gcagtgtgaa attgcgaaaa
    11941 cttgtgggca cctgaacccg ggaaggcagc accccatcca tctgcagcgc ttcgctgagg
    12001 tagggaacgt cgtcaaggcg gaaccccatc atcctcgcag cggtgaaatc cagcgaggat
    12061 acggaacgcg agcccagcag caggccgaat ttcctcaccc tgcctccgga ggggccgatc
    12121 ccgtccattc ccaggatgcc gtcgatgaag ctgtaagaaa gccgggcgcg ggtcagcccg
    12181 taaagcgcca gcaacagatg ggaaaaatcc acggaattgg gatagcggct gtggtaatcg
    12241 gtcttaatca ggccgggcac aaggccgtag aggtttttga gggcgccggt gaagcccacc
    12301 aggccatgag tcttgtattt gcctatgttg atcacgattc cgcactgcca gaagacctcg
    12361 gagatcttaa cgggaatccc gttccatttg agttcgcggt agccggaggt ggaaaggttc
    12421 accagcttca cgggatagcg gttggccagg tctttgagcc cgcaggcctg ccaaacctgt
    12481 tccacgtcgg ctgttccgcc ggggctgtcg ccgatccaga cctctttgcc gagctccaga
    12541 aaatggcgga tcaatgcttc cagcagcacc ggatgggtgg tgacagcccg ttccggggga
    12601 aaagcgccca aaagattcgg cttcagcaaa acccgtttgc tgcgctttat cctgattcgg
    12661 ttcgcgtggc ccagaaaatc ggccaccgcg gtctcaagct gcggtaggtc gtatgtttca
    12721 atcctgcgaa tctggacttt tggcatcagt cgaaatagag gaagttcttg cgcttgaagg
    12781 cgctgaaaaa gaggtctgcg gaaaggctga cctgggcgat gggggcctgc ccggccacgt
    12841 caatcaggcc ggaaaggttc acccgcacgt ccggatggtt ccaggaagtg cccagggtga
    12901 ggatatgctg attgtttttg cccacccggc cctggggcag cacggcagcc caccagatgg
    12961 aaatggtgtc cgaacaggcg tcagggatca ggaaatcccc ttcccaaacc tgcggatggt
    13021 agatatagcc ggcgcggtaa gtataacgtc tgacggtctt ttccatgccc gcgtggaagg
    13081 tgtaacggtc cttgaaggca tcgctgatgg cgctgcactg ctcataatcc acctcggcgg
    13141 tgaagacggc gttgtttttg ttgtaagcca cgccaagagc caggtttagg ggcagccggg
    13201 tatcgtattg ggcgaatttg aggtcccaat tcacgttctg ttgcggcacc atgctaaggc
    13261 cggcactcag ggattcgccg ctgtaaagta gccccagttg cggtctgagc acatatttgt
    13321 cgctaatgat gcgctcgaac gaacgcagga aagtcacgtc gcccagatgg tagatctggt
    13381 tgtgcaaatt cagccccacg tggaagttgt ccttgtgcca ggcagcgttg gcggtaagtt
    13441 ggttcaggaa aaagcttgga tagcgcatga tgaggccgta gcccatgttg acttccacgc
    13501 cgaaattatc caccttgatg ctttttggca tactataaag aaggccgtaa gtgaaattgg
    13561 ggccgatagt tccgccgccg ccaacgatcc ccaccggcac gggtgagagc atgttctctt
    13621 tggtcatgcg gtagtaaacg gaggtgtcgg ccatcgcgta aatatcgctt tccaccggag
    13681 gttttaccag catttccaga tgcagcgagg ccttgtccgg atggtagccg gcgggatttt
    13741 tgagcacgtt gtccactccc ccgggaatgg ccaggccggt gtagccgcgt cccatcgcgc
    13801 tggtggacat gtagttgcgc gtaaaactgt caaaaatggg attcacgtat tggggttggg
    13861 gaaagttcaa gacgtcgggg gattgggcag acagcgccgt gcccagcagc cccagcataa
    13921 gcagacaaat aatgtttctg cgcatggtta tatctccatt tatcgcttta caacaatggt
    13981 ttgagtgcgg tctttgccca ccgagaccaa ctcgatggga acctccacga ggtcctggac
    14041 cgcttccaga tattcttttg ccgttttggg caggcggtgc aggctgcgga ttttgccgag
    14101 gtcggcttcc cagcccggca ggctaaggta ttccggctcc agccgttcca ggatcagcgg
    14161 gtgcggtgta tagccgggga gttttttccc atcaagccag taacccgtgc aaatcttcag
    14221 ttccggcagg ccgctcaaca catccaaaag cgtgagtgcc agggcatcca ggccgttcag
    14281 tgaagcggtg tatttggcgg cgacggcatc gaaccagcca atccttctgg gccttccggt
    14341 ggtggcgcca aactcgttgc ccacagcgcg gatttgctcg gcagtctcat cgaaaatctc
    14401 agtgggaaaa ggtccttccc ccacccgggt gacgtaagct ttataaacgc caatggtttt
    14461 gtccagccag cgcgggggaa aaccgatgcc gatgctggcc cctcccgtgg tggtatttga
    14521 cgacgtgaca tagggatagg tgccgtaggt gaggtccagc aaggtgccct gggcgccttc
    14581 aaagagcatg gattctccgg ccagatagca attgcggagc agctcatccg cctgggccgc
    14641 aaagggtttc aggaattccc agacctcggc cagctctttc agttcgcatt tcaaatcgcg
    14701 cggcttaagg tcgtgatagg cgtaaaggct ggccaggcgc tgcttcagcc agtccggatg
    14761 tcccaagtct ccaaaacgca ggccgcagcg ggccgcctgg tccgaatagg cggggccgat
    14821 ccccttgccc gtggtgccga tcttgctggc tcccaattcc tcttccgtgc tgccgtccag
    14881 ctctttgtgc agcggcagaa ccagtccggc gcggacgtcg atgaacatcc tgccgccaaa
    14941 atctatgccc tgcttcatca gcgcccgcat ttcgccgcgc aggccgtagg gatcgatgac
    15001 cgttccggcg ccgatcacgc atttggtttt gggatagagg atgccggagg gcaccgtgtg
    15061 gaaaacatat ttgttccctt ccagaactat cgtgtgcccg gcattggccc caccctgaaa
    15121 gcgggccacc acatcggcat tcgcgcccag aaaatcgacg attttggcct tgccctcgtc
    15181 gccccacatg cagcccagca ccgttaatga actcatttat cgctccctta ggttcgtaaa
    15241 agcattattt tacccctatg ttttcctgtc aaatgctttt ttgggcttgg ggcgccgcag
    15301 gctcaaacgg gtttcaaaga tgcgattacg cgcggataaa tcggcttttg tggctttgaa
    15361 caggcctgat gtcattcccc gcaaaagagc gaagcgatgc tgtctggtcc ttaaggccct
    15421 tccttgtggt tcggattcgc ctcccgcaca atagccgcac ttgatgcgag tattatccgg
    15481 gaggcatggg agggggacgg tcgtgagggt taagggtgcg gcagacagga cttggcaggg
    15541 ggtaagtttc tgagtatcaa atgaaagaaa atccctccgg ctcccatcgc cgttactgta
    15601 tcagcggcaa gataatgctt gacatctgga cagcgggtcg aaagcttgat ttaaataggt
    15661 tttatcagcc tgaattattt ggactggcaa agcttaaagc taaagagagg tgtaaacatg
    15721 aaacgcatgt atttactgct cctgccgctg ttagcgctcc tgtttctcgc ggcttgcgaa
    15781 tccggcggca atttccgcgt gatcaaccga acctcatatc cggtgtacgt cacgctcgac
    15841 gatgaggcgg agtttgccat tcccgcaggc acggagcaca ctttcgaggt tgctaccgcc
    15901 aaccagcaca tcttcaatcc cgacgtgagc gtggaagttc cggtgcgcct ggtgggcgaa
    15961 actttccaga tctacgacga gggtaacgag gttttcaccg acaccaccag ggtggttatc
    16021 cacgccggga aaacaaccag cgtctttgtc gatcccaacc gtgccagttt caaggtggtc
    16081 aataacagca gccagtacat aatccaggcc aaattgttca agcacaattt cgtgggcgtg
    16141 gtcgcctcca acgatacggg gcccatagct ccgggaagat tcgtctatct gcctgtggaa
    16201 tatgccacaa gcagcaacaa cttttactac tatgccaccg ttcagatgga agacggggcc
    16261 acctacaatt acggtaacga aaccaacatc ctggccgttg accagcagtt tttgatcacc
    16321 ctcaccgacc ctgaataaag gaattaaatg aaccccaacg tcaagctttt cgcgaccctc
    16381 atcctgacgg ccctgctgac cgggcttttc gcccagccgg cggaaatgat gcaaatcccc
    16441 ctccagcagg accccgccgt tctttccggc cagcttgcca acggcctctc ctattacatc
    16501 atgcgcaacc ccaaacccgc caacctggcc gaattgcggc tctatgtgga tgtgggctcc
    16561 gtgaacgaag acgcggacca gcttggcctg gcacatttca cggaacacat ggccttcaac
    16621 ggcaccaaaa actttgccaa aaacgaggtg gtggaatacc tttcctccat cgggatgggt
    16681 tattacaacg gcctcaacgc catgaccagc tacgactaca cggtttacca actgaaaata
    16741 cccaccgatg acagcgccaa gctgaaccag gggctgctga tcctttccga catggctcac
    16801 caggtgagtt tcgaggcttc ggaaatcgaa cgcgaacgcg gcgttatcat tgaggaatgg
    16861 aggctgggac aggatgccgg cagccgggtc agagatgccc agaacgccgt tttcctggcc
    16921 ggttcgcgct acgcggaacg ttcgccaatc ggcaaacggg aagttctgga aagctttgag
    16981 catgacacca tcaaacgttt ttaccgcgat tggtaccgtc ccgacctcca aagcgtggtg
    17041 atcgtgggcg attttgagcc gcaagacatg ctggcgctgg tggagcggta tttcggcgtt
    17101 atacccaaac gtgaggaagt tcgccccaga gaggatttca gcgttcccga aaaccttgcc
    17161 ccccaggcca ttgtgaccac cgatcccgaa tatccgaaca acctgctgca ggtgatgtgg
    17221 aaaaaggacg tccagcggat gagcaatttg ggcgagtatt atgacaatct caagacttcc
    17281 ctgttctaca ccatgctcaa tctccgcctg gaagagaaaa gcaaaacagc cgatccgccc
    17341 tattccttcg ccgccgcaac cgaatatccg ctgctgcgaa cgatgtccgc ggcgatgatg
    17401 ttcgcgatgt acacccaagg caagtcggaa gaggctttgc gcaccgtcct caccgaggcg
    17461 gagcgggtgc agcgttacgg ctttttgccc agcgagttcg aacgcgccaa aattgacatt
    17521 ctgcgtggcg ccgagcagga tgtggctgaa cagggcaccc gtgaatccgg ccgcatcgcc
    17581 tgggaaatcg tggacgccgt ttccaaccag aatgtcttta ccagcgcgga attccgtcag
    17641 atgcttaccc aagaactgat ccaggccgtc accttggaag aggtgaacgc actggtggaa
    17701 aacctgatcc aggacaagaa catgttcatc ggcctggctg gcccccagaa agaaggattg
    17761 acctatccca ccaaagagcg ccttttggaa atcgccagcg gggtgaacag cttggaaatc
    17821 gagccctacg aagataaaac cgtgaacgag cccctgcttg aggtaatccc ggagccggtg
    17881 gcgataaaga aggaaagctt tgatgccaaa tccggcgtga acatctggac cctggccaac
    17941 ggggtgaagg tttatgccaa gaaaacggat ttcaagaacg acgaggtgct gctccaagcc
    18001 atcagccccg gcggattcag ccgctatgac acaaccgacg ttcccgctgc cagactggtt
    18061 tctggctata tcagcgatgc cgggttcggc aaattcgacg ccatctcgct gggcaaggcg
    18121 accgccggca aagtagccag ggtgaatctg agcgttgaca cttaccagga atctttcgac
    18181 gcttcctgtt cgcccaaaga catggaactg atgttccaga tgctgcacca gtacgccgtc
    18241 aatcccagat ttgaccgcgc tgattttgcc tcctacctcc agcgcacgaa aaccttttac
    18301 caaaacagga acctggaccc gatgaacgtg ttcatggacc gggtccagaa agagctctac
    18361 caaaaccatc cttatgccaa gaacctggcc gacgcggatt tcgacaccat cgacatggat
    18421 gcagtggaac ggattttccg ggaccgcttt gccgactttt cggatttcag cttcgtgatt
    18481 gtgggcaatt tcgacccgga actgctgaaa acctattgcc gcacctatct gggcaacctt
    18541 ccctgcggcg gcaggctcga aaagcccaag gatgtaaaca tccgcctgag aaagggctta
    18601 cagtccatct ggttcaatat gggctccaca gaccgcgcct tcgcgtccac cttactgaac
    18661 ggaaaataca actccagcac caaaaacaag gtgatgctgg acgccctgga gtttgtgctc
    18721 aatgaaaaac tgcgggaaaa catccgcgag gaacgcagcg gggtctattt catccaggcc
    18781 tggcaatcca ccaccagcta ccccgattcc cgctacaccg tcggcaccat gatgggttgt
    18841 gccccggcgc gtttgaaaga gctcaacgac gccatcttcg ctaccctgga cagcctccgc
    18901 gccggtcata tcgacaagca ctacctcgtc gcggccaacg ccactctccg gcagcagttt
    18961 gacgagaata tcaaatccaa ccgtttctgg atttaccagc tcaagtccga catctggcaa
    19021 ggcaggaagc tcgcctcatt cctggacgaa cccgctttct tcgatcagat cgacctgaaa
    19081 aacatcgcca aagccgccaa gaaatacctg cgcttcggta aaaaccaact gtcggtcttt
    19141 atgctgcccc aagtccagga gtgaatagag cggaagccag cccggaaagg aaacatttac
    19201 ctcatcaaat tggttgacac aaagccgcca tccaaaagta gggttgctgt atcagtataa
    19261 aatagtataa ataaagatag ataaggagtt atccacatgg ctgataagat caaagccact
    19321 atggacggca acaccgcagc ggcacacgtg gcctacgcgt ttgccgaagt ggctgccatc
    19381 ttcccgatta ccccgtcttc cacaatgggt gaacttgccg atgcctgggc cgcggacgga
    19441 cgcaaaaaca tcaacggcac cacggttgac gtgatcgaaa tgcaatctga agcaggagcc
    19501 gccggagcag tgcacggagc gctttccgcc ggtgccgtca ccaccacttt caccgcctcc
    19561 cagggcctga tgctgatgct gccgaacatg cacaagattg ccggagaaat gctgcccacc
    19621 gttttctatg tgaccgcgcg ttcccttgct gcgcagtcgc tctccatttt tggcgaccat
    19681 tccgacgtga tgagcgcccg caacacaggc tgggcgctga tttccgccaa cagcgtgcag
    19741 gaagtgatgg acctcggctt ggtggcccag cttgccacga tgaagacctc catccccttc
    19801 ctggtcttct ttgacggctt ccgcacctcc catgagctgc aaaaaatcga agttatcgac
    19861 tacgagacca tggccgagct caacgatccc gccctggttg aggctttccg cctgcgtgcc
    19921 ctgaatcccg agcgtcccaa ggtgaaggtt ggccagcagt ccccggatgt ctatttccag
    19981 ggcagggaaa ccaccaaccg gcattacatg gaagccccgg ccaccattgc tgccaccatg
    20041 gacgaagtgg ccgctaagat cggtcgtgcc tacaaacctt tcgactacgt cggccatccc
    20101 gaggctgaac atgtgatcgt ggccatgggc agcggctgcg aagcgatcga agaaaccatc
    20161 aactatctga acgcgaagcg cgggatgagg ctgggcctgg tcaaagtgcg tgtttaccgt
    20221 cccttcagtg tggagtattt cctcagcgtt ttgcctgcca gcgtgaaaaa tgtggctgtg
    20281 ctcgaccgca ccaaggaacc cggctctatc ggcgaaccgc tttatctgga tgtggtttcc
    20341 gctctcaaag accgccccgg tctcttcatc atcggcggtc gctacgggct ttccagcaaa
    20401 gacttcaccc ccacgatgat cctggcagtt tacaagcacc tgatggccaa aggcttccac
    20461 ggcttcactg ttggcatcga agacgacgtg acgaagctca gcctgccgct ggacgaatac
    20521 atcgacccca gccctgaggg aaccatcaac tgcaagttct ggggcctcgg ctccgacggc
    20581 accgtgggcg ccaacaagaa cagcatcaag atcatcggtg acaacaccga tctctatgtg
    20641 cagggctatt tccaatacga ttc
//
LOCUS       DAOOWR010000002        18090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_3116_length_18090_cov_4.571666, whole genome shotgun sequence.
ACCESSION   DAOOWR010000002 DAOOWR010000000
VERSION     DAOOWR010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 18090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18090
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3116_length_18090_cov_4.571666"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..985)
                     /locus_tag="PLB85_00100"
     CDS             complement(386..985)
                     /locus_tag="PLB85_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOU71756.1"
                     /translation="MKKITLISITILMLAGMLAAQGKDYPQLQRNPNCYGPGRNAKLA
                     PRPEWDGTSMDMLKELNLSEAQTKKLSDLQISQRKAMNSINAEIENLQIDLNNSLKSK
                     DFSSARKLNDQLHEKMRVKANTRIDHREQILRELSPEQQEKFRPMFMERQYGRPNGSK
                     HPMPCKLNELKPVQLHRHQRNMMNQDCCGCEDCHNKTEK"
     gene            complement(982..1434)
                     /locus_tag="PLB85_00105"
     CDS             complement(982..1434)
                     /locus_tag="PLB85_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71757.1"
                     /translation="MSKRLLTILLFISLFLNAGIIGGMIVMGFYRQYHIAHHYLPDSE
                     GIGSDRGRIQIPELKDPEVIALRNRFLSIKKELLQELAKDPVDEAKIKAIIEKSISAQ
                     SDMERALGYELLEYRQTLSADEAKKHFEGRLERMNRFSDRRNDRRKSK"
     gene            complement(1447..1890)
                     /locus_tag="PLB85_00110"
     CDS             complement(1447..1890)
                     /locus_tag="PLB85_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71758.1"
                     /translation="MRCSKARRQIELKLDNELGQSHALELHLKDCAACRAYQADAARL
                     QMLLRNQPQPEFPSWLHHRIMEQAARHDSKRIRLKHSFNLQTIPALAAIALSLFLGVA
                     IGKNVYRSVNPLPTNAKEIYSQNANKSDSLELAVFGESSVSGDLF"
     gene            complement(1944..2441)
                     /locus_tag="PLB85_00115"
     CDS             complement(1944..2441)
                     /locus_tag="PLB85_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU71759.1"
                     /translation="MRKEQFEEFLSAHEKRIYHYLLSLLGNDSDANDVVQMVFISFYE
                     HIDRVEEATALAYVYRIAYNKSMTFLKQKSRYVSLEPQSFERLPDTNKPQPEPDYQPL
                     HEAIRALPPRLASVIQLQYFEKMSYKEISEHLGISVKAVESLLVRAKKQLRKKLLKDK
                     SEGRV"
     gene            2641..5127
                     /locus_tag="PLB85_00120"
     CDS             2641..5127
                     /locus_tag="PLB85_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOU71760.1"
                     /translation="MRKTLCLAAILACLAWLGAEALLSPAEYNALHEMLQTAELSPDA
                     LCFEKDWDLSTKYKLDWQLRQLQDPWKGIDDMQVLRETCQLDGPEALPTLLRHLGGIA
                     FNLDGGRFSSLYPASKSLYTQQLSTQVKKPEDIFAWLESAYDYLETELEPAFAAFSPE
                     EKKMLLSFWHWQFIESEEIDKYNAYYEEQGLPKYSDMDEKRVRELIGKLDRQALLSTG
                     IQFLAISDALLERAQTLLFRNKKILIRNTRHGLMAIGTNGDDVYGGQAFQNLCFLLDP
                     AGNDLYETGLGSSVDRAFCLQLDLAGDDVYRSNVPVAMFNSSFGLVCTYDLAGNDLYR
                     TNDFSFSAFMGINLHQDLDGDDIYQSGLFSQGAAMCGIALLLDGAGNDSYQASVSAQG
                     FGSTYGAGVLLDKEGADNYSLGGKYYHEPLMPLDFITLGQGMGLGLRPDLAGGLGLLY
                     DGAGNDRYLGGVYAQGSGYWYATGALIDEAGNDVYSAVYYPQGSGIHLACGFLLDAEG
                     DDAYQSRHGPGQGAGHDWALGVLIDGAGNDSYSIEGGNGLGLTNSVGLFVDKSGNDRY
                     ERINPQNYGGANFTRSAGGIGLFLDAGGTDSYPDSLKANNSIWQSGTYGVGRDAEINQ
                     VAKTTVEELAEAAALPDSTDSIETIFAAASEWEVGSAVQRVRTARGILLSRAEEAIPY
                     VLENKLNTTSGLEYRALEALAEDSEEFISELYKVIDLPDSLAAKNALALISGVGDSLL
                     VEPVERLMRQKKYESTCISVLSGVHTERSVELLRDYIFHPSERFRYIAARSLKQIKHP
                     SARAALELMRGDCSFLVQSLLRSLTEVQQP"
     gene            5124..5756
                     /gene="rsmG"
                     /locus_tag="PLB85_00125"
     CDS             5124..5756
                     /gene="rsmG"
                     /locus_tag="PLB85_00125"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU71761.1"
                     /translation="MKSSRAAFSQFLDELKLPGRDDILAGFDSYHALLLEVNSRVNLF
                     SRHAPADELWTKHFLDSLLPLKCVDFNGAKVLDFGSGGGLPGIPVKLAVPGCRMTLLD
                     SVLKKTRALEELVARLELADCEVVCSRLEDYSARGGFDYILCRAVKLEQRYLKPLRNL
                     LAPGGQLLCYKAKDWADIAPLKPLELLRMELEYGSRAIFALGKGQLKTGS"
     gene            5766..6536
                     /locus_tag="PLB85_00130"
     CDS             5766..6536
                     /locus_tag="PLB85_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU71762.1"
                     /translation="MARTITIVNQKGGVGKTTTAVNLAAGLAVLEKKTLLIDFDPQGN
                     ATSGVGLDKDNLELQVYDALIGKVPLRETILPTSTQNLWCVPGNIDLTGAEIELVQEF
                     AREHKLREALEPVLADFDYIIIDCPPSLGLLTVNALTAATDLLVPIQCEYYALEGVSQ
                     LLSTIRLIQKNLNPGLNIIGILLTMFDRRVNLSMQVAREVRHYFKEKVFRTIIPRNIK
                     LTEAPSFGKPIFLYDIRSPGAMSYLNLATEIIERTQKA"
     gene            6533..7384
                     /locus_tag="PLB85_00135"
     CDS             6533..7384
                     /locus_tag="PLB85_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU71763.1"
                     /translation="MNERLGRGLSALIPQAQDRPETGSNLGTLPIEQIKTNPYQPRRV
                     FDPDALQELAESIKANGIIQPLIVNKTAASDYELIAGERRLQAARLAGLESVPVVIRS
                     VSQKEQLQLAIVENVQREDLDPIEEACAYQTLADDFKLTHQEVAQIVSRDRATVSNSI
                     RLLKLPLEVQSMVSSGLLSPGHARAVLAVEPELQPFFAQYIIQYRLNVRQAEAKAKTF
                     APSQAKEAPANREWSQSLERELSGAFGLKARVSGQAAKGKITLSYSSAEELARLQELL
                     KLIRKDI"
     gene            7381..8451
                     /gene="rfbB"
                     /locus_tag="PLB85_00140"
     CDS             7381..8451
                     /gene="rfbB"
                     /locus_tag="PLB85_00140"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU71764.1"
                     /translation="MNRIIVTGGAGFIGANYIHHLFEDPDFQGSVLNFDKLTYAGNLD
                     SLVEIEAAFPERYRFVKADICDSEAVARVFAEFQPDTVVNFAAESHVDRSIDGPMEFI
                     NTNVLGTAVMLDCALRYWRGLDEAGKKAFRFHHVSTDEVFGSLGDEGLFTESTPYDPS
                     SPYSASKAGSDHLVRAWHRTFGLPVLLSNCSNNYGGYQFPEKLIPLMILNCLEHKPLP
                     VYGKGLNVRDWLYVRDHCDAINTIIRSGKLGETYNVGGHNEMKNIEIVTTICRLLDEL
                     RPSDKLSSYTELITYVADRPGHDLRYAIDADKISRELGWVPKETFETGIRKTVLWYLE
                     HEHWWRAIQSKKYQQQRLGLGN"
     gene            8535..9101
                     /locus_tag="PLB85_00145"
     CDS             8535..9101
                     /locus_tag="PLB85_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71765.1"
                     /translation="MTKTYRHFAVLIALAAALAISACSPNSWLNSALPNPAVVAQPAS
                     SSQVPDAGTDAHFVRVDEYFVMEQPLTTQPFVFASLGRMQSLPTPDHNNQGRFLRLLD
                     SQQVWTSHFSKTRIAKSSDLVLDKEVFFLQLTDPNGFYRAPQTLAETSGGWWIKARIT
                     DLSTKNNGYVKVSGGYNVHLNAIRVAVN"
     gene            9648..11597
                     /locus_tag="PLB85_00150"
     CDS             9648..11597
                     /locus_tag="PLB85_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU71766.1"
                     /translation="MKHIVLCIAACVLAAALCAQWSTDPYAPSIIAGFDAEQVIPKVA
                     IVPNGNTYICRFDNQSGGYRVYLDLFDIYGNRIWTESLLISSHTQMSWLTEYDLGVDT
                     EGNAIIVFQDIRNAGVNNVVAYKVNPAGEFLWGADGIALSNDTNADYGNMSPVCFCSA
                     DGSSYFAWQRLGAVTSVVINRLSADGQKLWGEWGTTFTATEGSYTWPQIIQSEGNDIL
                     LKLFHDTGPFWSPTRHILVMKGTPDGTFPWTVSATEAGGIPAWEQLIPFVGDGAGGAV
                     LAWYDDRDSNLDNDVYCQRIDSQGNATMGLNGSLVNVSLPKQQFYPEVAVDAANQQVY
                     VFYRETDADQNNFGLARQLMSFSGDCLWGQAGPTIVNIGGESCNTIAAHWTTQGAVFL
                     YERGANLYASCWTSSGDFAWASSQVGVAQTTDPKQHFDCDHHPDNWCVLSWEQGYSGF
                     DIYAMRINGNGSLGPQFLPPRNLTAEQQPPSSVILSWQHPSPYVEPDQYCIFMNDELA
                     QTVAGDVLTYTVTDLGSGTHEFYVKARYGDNYSDPSNSVTVTIVAADDPILPSVEAAP
                     RLTPNPFSGTATLSFVGKNSSTACVTLYNAKGQKLGQRDCAVIAGTNEITIDPRSLGL
                     RESGVFFLSLRTGGQVSLLKGLFIK"
     gene            11789..12493
                     /locus_tag="PLB85_00155"
     CDS             11789..12493
                     /locus_tag="PLB85_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU71767.1"
                     /translation="MSKQIFVTEDETDILELVCMKLETEGYSTLGFTRAQDLLDRLRQ
                     ELPELILLDLMLPDLDGLEACRRIKSNPAWENVPIIMLTARSDIEDKVRGLEFGADDY
                     ITKPFDSKELLARVKAVLRRSGWESSRNVLNINSDFRLDFNRYEAWIRDHRVDLTLTE
                     FKILQLLTRRPGWVFKRSQILDHLWGNDKIVLERTVDVHIRNLREKLGDQAYMVKNIR
                     GVGYKFEPDQSSGSDE"
     gene            12486..13829
                     /locus_tag="PLB85_00160"
     CDS             12486..13829
                     /locus_tag="PLB85_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU71768.1"
                     /translation="MNKTSLGTLLLLAGVVILAAVAVLALLDVGPWLLISVSAGVAGI
                     ALYLVHFWLNRDLRHLWKRAEELKPQERKLRFGELRTSELHDLAQKLNQALASFDQAI
                     GHLAVHRTELRLLLGTLEDPLWSQDDDGNVLWANAPFHKLFPAFDSSREQSYREIIRD
                     PELQEAILSSGNAGEQLPLSLNIAGHSYMLSSSRSVSNRRYVFILHNIDAMERTARMK
                     KDFIVNLAHELRTPLTAIKGFTEAMQDTPGQDHTRHQKIILSHTNRLIHLIRDLEQLI
                     RLESISRLEASQIELKPFFANLSLILEPDIQAKGLSLSVELEEGLDRMVCDPFRFEQI
                     FINLVQNSLRYTDTGGITIYVGKEGENTVFTISDTGRGIQNEHLDRIFERFYVADNSR
                     NRDFSGTGLGLAIVKHIVVLHHGEISVSSEMGRGTTFRISIPPLAERKNESNQPS"
     gene            13807..14322
                     /locus_tag="PLB85_00165"
     CDS             13807..14322
                     /locus_tag="PLB85_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU71769.1"
                     /translation="MNQINQAEAMESCSNLQADRDLLVNENIGLAYSFAKNCQNRGLP
                     LEDLRQEALLGLLEAAEHFEPERGLKFGTYAGYWIKKRLLAALEQENKAVRNTEPLGE
                     RDLADHKQQVDARIESSITFPSAMPDLERGVLQLSLNEGLALKEVASRLGISVERVKQ
                     LRGKALRRLRS"
     gene            14818..15951
                     /locus_tag="PLB85_00170"
     CDS             14818..15951
                     /locus_tag="PLB85_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71770.1"
                     /translation="MKKLLLATLVGLFLLPALLGALELKISGEMWNRWTLEQGKVFAD
                     TSLTAYKKEITKNFFSLERGYVGMEAKFSQSMKGRFTVDIFSSDILKDGAGLKLKYAY
                     LDFSNLIPIPDLTTTVGLQKVYFGSIYDWSYNLIGKAPTDEYKYANSADYGISLNGYL
                     PGGWGEYQLGMYNGEGYKNYGSNLKDNIDPAFLANLRIVPTAGLTIGGSVMANGSERK
                     LKLADATENSSYNTQLLADGILRAAFGPLDIWAEYLYKDISYAPNFSAKDYSATCFSV
                     FPTLKLKSLIGADIDLQGRYDRSDETRMPDSKKKNLLNAWTGGLNFNFMADDSGSPAL
                     QAQLNYVRKDYDEDASGTDYANGKKDTDTVMLQLKWRFASTIY"
     gene            15963..16775
                     /locus_tag="PLB85_00175"
     CDS             15963..16775
                     /locus_tag="PLB85_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU71771.1"
                     /translation="MNKKYIIVALILLLAISFAVAKSRKITCSGSTTVLPIAQATAEA
                     YMDKHPDVNISIRGGGSGVGIAALQNGSADIANSSRVMKAKEISQAKSKGINPTPYAV
                     ALDGIAIVVHKSNSVANLSIAQIRDIYNGKIKNWSEVGGPKLPIVVISRDVASGTFEV
                     FNEKALGGSKVVSSAQLLASNNAVASTVGSTPGGIGYIGLGYLTEEIKTVKVNSITPN
                     TKTVQDGSYPLARKLYMYTNGKAKGDLGSYIAFIQSDAGQKIVVEQGFIALK"
     gene            16849..17718
                     /gene="pstC"
                     /locus_tag="PLB85_00180"
     CDS             16849..17718
                     /gene="pstC"
                     /locus_tag="PLB85_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOU71772.1"
                     /translation="MKTFKEKSFQTITYTAALFTVACLLGIIFGLFREGLPIFKHVGL
                     KEFLFTNNWYPTHAEPGFGALALIVGSLVVTLGSLIIAIPLGLGAAIFLSEMAGPRLR
                     EITKPVIELLAGIPSVVYGLFGMAFLAPLVREWFDLDTGLNVFTASLILGVMIVPVIC
                     SMGEDALSSVPKNLREASLALGATRAETIFKAVIPAAKNGLAGSVLMGFGRAIGETMV
                     VLMVAGGAARIPSSIFDPVRPLTSTIAAEMGETVMGDLHYQSLFALAIILFLITFVSN
                     LITESMLLKRGKK"
     gene            17715..>18090
                     /locus_tag="PLB85_00185"
     CDS             17715..>18090
                     /locus_tag="PLB85_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71773.1"
                     /translation="MNKRKLMNSLVTGIMALMLVLTALFLVLFLVRMFSQGAKVLSWD
                     FLFTAPRDAMRAGGIYPALVGTFLLTALSMVVALPLGILTAIWLTNYGRPVWLVNILR
                     IAINTLAGTPSIIFGLFGMAMFS"
BASE COUNT         4083 a         5165 c         4834 g         4008 t
ORIGIN      
        1 taaaccacca cccagcccca cacccacgtc attccgggct tgacccggaa tccaggtttt
       61 tgtaacgtat ggatcccgga tctaagtcac ctcacggact cgatgacgtc cgggatgacg
      121 tatgttgggt tcttgcggcc aaggtcttgc acgtcattcc aggcttgcct gtgaatccag
      181 aaacaaccat cacccagccc taaacctacg tcattccggg cttaaaccgg aatccagttt
      241 acgaggggct ttaggctccc tcgctatcgt tatatcgccc tactgttata tcaaccggat
      301 tacccactcg gtttgaaaga gcaaaaagga acgccaggcg gctaaacccg gcgttcttct
      361 atctttactg aggaggggga tggatttact tttctgtttt gttgtggcaa tcttcgcagc
      421 cgcagcagtc ctggttcatc atgttgcgct ggtggcggtg gagctgcacg ggcttaagtt
      481 cgttcagttt gcagggcatg gggtgtttgg agccgttggg ccgtccgtac tgccgttcca
      541 tgaacatggg gcggaatttt tcctgctgtt cggggctgag ttccctgagg atctgttcgc
      601 ggtggtcgat gcgggtgttg gcttttacgc gcattttctc atgcagttgg tcgtttagtt
      661 tgcgggctga tgagaagtct ttgcttttga gggagttgtt gaggtcgatc tgcaggtttt
      721 cgatttcggc gttgatactg ttcattgcct tgcgctgcga gatttgcaga tcgctgagtt
      781 ttttggtttg ggcttcgctg aggttgagtt ctttcagcat gtccatggag gtgccgtccc
      841 attcggggcg gggtgccagt ttggcattgc gtccggggcc gtagcaattg gggtttctct
      901 gcagttgggg gtagtctttc ccctgggcgg ccagcatgcc ggccagcatc aggatggtta
      961 ttgagataag ggtgattttt ttcattttga ttttctcctg tcgtttcttc tgtcgctgaa
     1021 gcgattcatg cgttccaggc gtccttcgaa gtgttttttg gcttcgtcgg cgctgagagt
     1081 ttgtctgtat tccaaaagtt catagccgag ggcgcgttcc atgtcgcttt gcgcgctgat
     1141 cgacttttcg atgatggctt ttatcttggc ctcgtccacg gggtctttgg ccagttcttg
     1201 caatagttct tttttgatgg agaggaatct attccgcagt gcgataactt cggggtcctt
     1261 cagctcaggg atttgaatgc gtccccggtc gcttccgatg ccttcggagt cgggcagata
     1321 gtggtgggcg atgtggtatt ggcggtagaa tcccatcacg atcatgcctc cgatgattcc
     1381 agcgttcaga aacagcgaga tgaaaagcaa aatggtcagc aggcgtttac tcattgtttc
     1441 ctcactttag aaaaggtcgc cggagacgga actttcgccg aatacggcca attccaggct
     1501 gtcggatttg ttggcgtttt gggaatagat ttccttggcg ttggtgggca gggggttcac
     1561 gctgcggtac acatttttcc cgatggccac acctaggaag aggcttaggg ctatggccgc
     1621 gagcgcgggg atggtttgca aattgaagct gtgtttcagg cgaatgcgtt tgctgtcgtg
     1681 ccgggctgcc tgctccatga tgcggtggtg cagccaggac gggaattccg gttggggctg
     1741 gttgcgaaga agcatctgaa gccttgctgc atcagcctgg taagcgcggc aggcggcgca
     1801 atccttaaga tgcagctcca gggcgtggct ctgccccagc tcgttgtcga gcttgagttc
     1861 gatctggcgt cttgccttgc tgcatctcat ggtttcctct ttttgggtgc cttatcggcg
     1921 tccctgtttt gcttattcat agtctatact cttccttcgc ttttatcctt caacaatttt
     1981 ttccgaagtt gctttttggc cctcaccaac agcgattcca ccgctttcac gctgatgccc
     2041 agatgctcgg aaatctcctt gtagctcatc ttttcgaagt attgaagctg gatgacgctg
     2101 gccagacggg gcggaagggc gcggatcgcc tcatgcagag gctggtaatc cggctcgggc
     2161 tggggtttgt tggtgtcggg aaggcgttcg aatgattgtg gttcgaggct cacatagcgg
     2221 cttttttgct tcaggaaggt catgctctta ttgtaggcga tgcggtaaac gtaggccagg
     2281 gcagttgctt cctccacgcg gtcgatatgc tcgtaaaacg agatgaagac catctgcacg
     2341 acatcgttgg cgtcgctgtc gttgcccaaa aggctcagca gataatggta aatccgcttt
     2401 tcgtgggcgc tgaggaattc ttcaaactgc tcttttctca taaaccgtcc ttctaccaaa
     2461 atacaccgct ctgggtttga acctgcaaaa aatactcagg ctgaggtgtc aagccttttc
     2521 ccaaaattga ggcggcaagg ctgtttcagc ccctttatca actatattgt aggcgctgat
     2581 agttgttgac acatttggcc ggctttcatt actggctttt caagtaagga gattaacctg
     2641 atgcgaaaaa ccctctgtct ggcagcgatt ttggcctgct tggcctggct cggcgccgaa
     2701 gccctgctaa gcccggcaga atacaacgcc ctgcatgaaa tgctgcaaac ggctgaactc
     2761 agccccgacg cgctttgctt cgaaaaggat tgggacctaa gcaccaagta caagctggac
     2821 tggcagttgc gccaacttca ggatccctgg aaaggcatcg atgacatgca ggtcttgcgc
     2881 gaaacctgcc aactggatgg cccggaagct ttgccgactc tgctgcggca tctgggcggc
     2941 atcgctttca accttgatgg aggaaggttc agcagcctct atccggcctc caagtctctt
     3001 tatacacagc agctctccac ccaggtgaaa aagccggagg acatattcgc ctggctggaa
     3061 agcgcttatg attatctgga aaccgagctt gagccggctt ttgccgcctt cagcccagag
     3121 gaaaagaaga tgctgctcag cttctggcac tggcagttca tcgaaagcga agagattgat
     3181 aaatataacg cttactacga ggaacaaggc ctgccaaaat acagcgatat ggatgaaaag
     3241 cgggtccggg agttgatcgg aaaactcgac cgtcaggctt tgttgagcac cgggatccag
     3301 tttctggcca tctccgacgc cctgctggag cgggcccaaa cccttctgtt cagaaataag
     3361 aaaatcctca tccgcaatac caggcacggc ctgatggcaa ttggaacaaa cggcgacgat
     3421 gtctatggcg gtcaggcatt ccagaacctc tgttttctgc ttgaccccgc cggcaatgac
     3481 ctttacgaga cagggctggg aagctctgtg gaccgggctt tctgccttca gctcgatctg
     3541 gccggcgacg acgtttaccg cagcaacgta cccgtagcga tgttcaactc ctcctttggc
     3601 ctcgtctgca cctatgatct ggctggcaac gacctctacc gcaccaacga tttttcattc
     3661 tccgccttca tggggatcaa ccttcatcag gatttggatg gagacgacat ctaccaaagc
     3721 ggtctcttct cacagggcgc ggcgatgtgc ggaatcgcgc tgctgctgga cggcgccggc
     3781 aacgacagct atcaggccag cgtgtccgcc caaggctttg gcagcaccta cggcgccggt
     3841 gtcttgctgg ataaggaggg cgcggacaac tacagcctgg gcggcaaata ctatcacgaa
     3901 ccgctaatgc cgctggactt tatcaccctc ggccagggca tgggactggg gctgaggcct
     3961 gatctggccg gcggtttggg cctgctctat gacggcgctg gaaacgaccg ctacctcgga
     4021 ggcgtttatg cccagggttc cggctattgg tatgccacag gagcgcttat cgacgaggcc
     4081 ggaaacgacg tttacagcgc ggtttactat ccccagggct ccggaattca cctggcttgc
     4141 ggattcttgc tcgacgcgga gggcgatgac gcctaccaga gccgccacgg accaggtcag
     4201 ggcgccggac acgattgggc tttgggcgtt ttgatcgacg gggcgggaaa cgattcctat
     4261 tctatcgaag gcggtaacgg cttggggctc accaactccg tgggactttt tgtcgataaa
     4321 agcggcaatg accgctatga gcggatcaac ccacaaaact acggcggcgc caacttcacg
     4381 cgcagcgctg gcgggatcgg gctctttctg gatgccggcg gcacggacag ctatcccgac
     4441 agcctcaagg ctaacaacag catctggcaa agcggaactt acggcgtggg acgcgacgca
     4501 gaaatcaacc aagttgccaa aaccaccgtg gaagaactgg ctgaagctgc cgccctgccg
     4561 gacagcacag acagcatcga aacgattttc gccgccgcct cggaatggga ggtgggatcg
     4621 gcggtgcaaa gggtccggac cgcccgagga attctgctca gccgcgccga agaagccatc
     4681 ccctatgtgc tggaaaacaa gctgaacacc acttccggcc tggaataccg ggccttggaa
     4741 gccctagccg aggattctga ggagtttatc agcgaactct acaaagtcat cgacctgccc
     4801 gacagcctgg ctgctaaaaa cgcgctggcc ctgatttccg gcgtcggcga cagtctgctg
     4861 gtggaaccgg ttgagcgcct gatgcggcag aaaaagtacg aaagcacctg catttctgtg
     4921 ctgtctgggg tccacaccga acgcagcgtg gaactgcttc gggactatat cttccatccc
     4981 agtgagcggt tccgctacat cgcggcccgt tcgctaaagc agattaaaca cccatccgcc
     5041 cgcgccgcgc tggagctgat gcgcggggac tgttcttttc tggtgcagtc gcttttgcgc
     5101 agcctgaccg aggtccagca gccttgaaaa gctccagggc cgctttttcc cagtttctgg
     5161 atgagctgaa gctgccgggg cgtgatgaca tcctggccgg ttttgacagc tatcacgctc
     5221 tgctgctgga agttaactcc cgcgtaaacc tcttttcccg ccacgcgcca gcggatgagc
     5281 tctggacaaa gcatttcctt gacagcctgc tgcctctgaa atgtgttgat tttaacggtg
     5341 caaaagtgct cgatttcggc tccggcggcg gtttgcccgg cattccggtg aagctggccg
     5401 tgcccggatg ccgcatgaca ctgttggatt cggtgctcaa aaagacccgc gctcttgagg
     5461 aactggttgc caggctggag cttgcggatt gcgaagtggt gtgctcccgg ctggaggatt
     5521 actccgccag gggcggtttc gactacatcc tctgccgcgc cgttaagctg gagcagcgct
     5581 atctgaagcc gctacggaac ttgctggctc cgggtggcca attgctctgc tacaaggcga
     5641 aggactgggc ggacatcgct ccgctcaagc ccttggaact attgaggatg gagctcgaat
     5701 acggcagccg agcgattttc gcgctgggaa aaggacaact gaagactgga agttagcata
     5761 gatttatggc aagaacaata acgatcgtga accaaaaagg cggggtggga aaaaccacca
     5821 cggcggttaa cctggccgct ggcctggctg tgctggaaaa gaagaccctg ctcatcgatt
     5881 tcgatcccca gggcaacgcc accagcggag tgggcttgga caaagacaat ctggaactgc
     5941 aagtgtacga cgcgcttatc ggcaaagtgc ccctgcggga aaccattctg cccacctcca
     6001 cccaaaacct ctggtgcgtg cccggaaaca tcgacctcac cggcgccgaa atcgaactgg
     6061 tacaggaatt cgcccgcgaa cacaagctcc gcgaagcgct ggaaccggtt ttggccgact
     6121 tcgattacat tataatcgac tgcccgccct cgctgggcct gctcacagtg aacgccctca
     6181 cagcggcaac agacctgctg gtgccgatcc agtgcgaata ttacgccctg gagggggtaa
     6241 gccagttgct ttccaccatc cgcctgatcc agaaaaacct caatcccggc ttgaatatca
     6301 tcggcatcct gctcaccatg tttgaccgtc gcgtgaacct ctccatgcag gtcgcgcgcg
     6361 aggtgcggca ctatttcaag gaaaaggttt tccgcaccat cattccccgc aacatcaagc
     6421 tcaccgaggc acccagcttc ggcaaaccca tatttctcta tgacatccgc tctcccggcg
     6481 ccatgagcta tctgaacctg gccacggaga taatcgaaag gacacaaaaa gcatgaacga
     6541 acgcctcgga cgcggtttga gcgccctgat cccccaggct caggacaggc cggaaaccgg
     6601 atcaaacctg ggcaccctgc ccatcgaaca gatcaagaca aacccttacc agccccgccg
     6661 ggttttcgac cccgacgcct tgcaggaact ggctgaatcc atcaaggcca acggcatcat
     6721 ccagccgctg atagtgaaca aaaccgccgc ttcggattat gagcttatcg ccggcgaaag
     6781 gcgcctgcaa gcagcgcggc tggcgggcct ggaatccgtt ccggtggtga tccgcagcgt
     6841 tagccagaaa gaacagctcc agctcgccat cgtggaaaac gtccagcgtg aggacctcga
     6901 cccgatcgag gaagcctgtg cctatcagac tctggcggac gatttcaaac tcacccatca
     6961 ggaagtggcg caaatcgttt cgcgcgaccg cgccacagta tctaacagca tccgcttgct
     7021 caaactgccg ttggaagtgc agtcaatggt ctcctccggc ctcctcagcc ccgggcacgc
     7081 gcgggctgtt ttggcggtag agccggagtt gcaacccttt ttcgcgcaat atattataca
     7141 atacaggctc aacgtccgcc aggcggaggc caaagccaaa accttcgccc ccagccaggc
     7201 aaaggaagcg cctgccaacc gggaatggtc ccaaagcctg gaacgcgaac tgagcggagc
     7261 atttggcctg aaagccaggg taagcggaca ggcagccaaa ggcaaaatca ccctcagcta
     7321 ttccagcgcg gaagaactgg cccggctaca ggaactgcta aaactaataa ggaaggacat
     7381 atgaacagga ttatcgtgac cggaggcgct ggcttcatcg gcgccaacta catccaccac
     7441 ctctttgaag accctgattt ccagggcagc gtgctcaact ttgacaaact cacctatgcc
     7501 ggcaatctgg acagcctggt tgagatcgaa gcagcctttc cggagcgcta tcgatttgta
     7561 aaagctgata tttgcgactc cgaagcagtg gcccgggtat tcgctgaatt ccagcccgat
     7621 accgtggtca attttgccgc agaatcccac gtggaccgtt ccatcgacgg gcctatggag
     7681 ttcatcaata ccaacgtcct gggaaccgcc gtgatgctgg attgtgccct gcgttactgg
     7741 cgcggcctgg atgaagcggg caaaaaggct ttccgcttcc accacgtctc caccgacgag
     7801 gttttcggct ccctgggcga cgaaggcctc ttcacggaaa gcacgcccta tgacccgtcc
     7861 tcaccctatt cagcttccaa agcgggttcc gaccatctgg tgcgcgcctg gcaccgcacc
     7921 ttcggcctgc cagtgctgct ctccaactgc tcaaacaact acggcggcta ccagttcccg
     7981 gaaaaactga ttcctttgat gattctcaac tgcctggaac acaaaccctt gcctgtttac
     8041 ggtaagggac tcaacgtgag ggactggctc tatgtgcgcg accactgcga cgccatcaac
     8101 accatcatcc gctccggaaa actgggcgaa acctacaatg tcggtggcca caacgagatg
     8161 aaaaacatcg aaatcgttac cactatctgc cggctgctgg acgaactccg gccttccgac
     8221 aagctttcat cttacacgga actcatcact tacgtcgccg accgtcccgg ccatgacctc
     8281 cgctacgcca tcgacgccga caaaattagc agggaactgg gctgggtgcc aaaggaaacc
     8341 tttgaaaccg gcatccgcaa gaccgtactc tggtatctgg agcacgaaca ctggtggcgc
     8401 gccatccaga gcaagaaata ccagcagcag cggctgggac tgggaaactg aacgccccaa
     8461 aaatccggcg caggatttcc ggccgaaccc ggttatatag ttaaaaagac ctttctgaag
     8521 ggaggtgtga cataatgact aaaacttata gacactttgc cgtcctgatc gcgcttgcgg
     8581 cggccctggc catctcggcc tgcagcccta attcctggct taattccgct ctgccgaatc
     8641 ccgctgtggt ggcccaaccg gctagcagtt cgcaggtgcc ggacgctggc accgacgccc
     8701 atttcgtgcg ggttgacgaa tatttcgtga tggaacagcc cctcaccacc cagcccttcg
     8761 tgttcgcgtc actgggcagg atgcaaagcc ttcccacacc ggaccacaac aaccagggcc
     8821 gcttcctgag gctgctcgac agtcagcaag tctggaccag ccacttctcc aaaacccgca
     8881 tcgccaaaag cagcgacctc gttttggata aagaggtttt tttcctgcaa ctcaccgatc
     8941 ccaacggctt ttaccgcgcg ccgcaaaccc tcgcggaaac cagcggcggc tggtggataa
     9001 aggcccggat aacggacctg agcaccaaaa acaacggcta cgtcaaggtc agcggcggct
     9061 ataacgtcca tctgaacgcc atccgggtgg ctgtgaacta ataatatctc tcctcaaatc
     9121 agccgggcta actggtccgg cctttttttg ttcctgtctt tcggctcttt tcaggtcggg
     9181 caagttagcc cggagagcga cagaaatcaa tcccggcatt tctgtcgtcc acaaggggct
     9241 aagagaatcc attgtcgagg ggttccgttc gcttcgctca ctgcataccc tcgctatggt
     9301 ctgccacccc tacgggctcg tagaggagag tcgtaactgc ttaccttcca ctatttccag
     9361 ccagccacac ccttaacgct cgatttcatc cctctcccat gcctctctcg agattcccgc
     9421 atcaaatacg ggcattatgc gggaggcgcg tctttgggac aggaaagggc cttaacggcc
     9481 agagcactcc gcaccggtaa accggctgcc aaccaccact ttcgtatgac gcctaccagg
     9541 ctgggaaaga acgagttaca cgcccggttt gaaatagagc caaatatacc ttgactaaaa
     9601 atcccccaaa gcaaataatg gagtcattca attaatcgag gtgtagcatg aaacacattg
     9661 ttctttgtat tgctgcctgt gtcttagcgg cagccctctg cgcgcaatgg agcaccgatc
     9721 catacgctcc ctccatcatt gccggtttcg atgccgaaca ggtgataccc aaggtggcca
     9781 tcgtgcccaa cggcaacacc tacatctgcc gttttgacaa ccaatccggc ggttacaggg
     9841 tttatcttga cctcttcgat atctacggaa accgcatctg gacggagagc ctgctcataa
     9901 gttctcatac ccaaatgagt tggctcaccg aatacgacct cggcgtggat accgaaggca
     9961 acgccatcat cgtcttccag gacatccgca acgccggtgt aaataacgtg gtggcctaca
    10021 aggtcaaccc cgcgggcgaa ttcctctggg gcgccgacgg catcgccctg tcgaatgaca
    10081 ccaacgctga ttacggcaac atgtcgcccg tctgcttttg ctctgctgac ggcagcagct
    10141 actttgcctg gcagcgcctg ggcgcggtca catccgtcgt gatcaaccgc ctcagcgccg
    10201 atgggcagaa gctttggggc gaatggggca ccactttcac agccaccgaa ggcagctaca
    10261 cctggccgca gatcatccag agcgaaggta acgacatact gctcaagctt tttcacgata
    10321 ccggtccttt ttggtcgccc acgcgccaca tcctcgtgat gaagggcact cccgacggca
    10381 catttccctg gaccgtttcc gcaaccgaag ccggcgggat tcccgcttgg gaacagctca
    10441 tcccctttgt cggagacgga gccgggggcg cagtgctggc ctggtatgat gatcgcgaca
    10501 gcaatctgga caacgacgtc tattgtcagc gcatcgactc gcagggaaat gccactatgg
    10561 gtttgaacgg cagcctcgtg aacgttagcc tcccaaaaca gcaattctat ccggaagtcg
    10621 ccgtggatgc cgccaatcag caggtttacg tcttttaccg cgaaaccgat gccgaccaga
    10681 acaactttgg cctcgcacgg caactgatga gtttcagcgg agactgtctc tggggccagg
    10741 ccgggccaac catagtgaac atcggcgggg aatcatgcaa caccatcgcc gcccactgga
    10801 ccacgcaagg cgcggttttt ctctatgaaa ggggagcaaa cctctacgcc tcctgttgga
    10861 cttcttcggg cgattttgcc tgggcctcaa gccaggtggg cgttgcccaa acaaccgatc
    10921 ccaagcagca cttcgattgt gaccaccatc ccgacaactg gtgtgttttg agctgggaac
    10981 aaggttactc gggtttcgat atctacgcca tgcggatcaa cggcaacggc agcctcggac
    11041 cgcaattttt gccgccgcga aaccttaccg ctgaacagca gccgccgtcc agcgtcatac
    11101 taagctggca gcatccctct ccctacgtcg aacccgatca atactgcatt ttcatgaatg
    11161 acgaactcgc ccagaccgtc gctggagacg tcctcaccta cactgtcacc gaccttggca
    11221 gcggcactca cgaattctat gtgaaagccc gctacgggga caactactcc gatccctcga
    11281 actcagtgac ggtcaccata gtcgcggctg acgatcccat ccttccttca gtggaagcgg
    11341 cgccccgcct cacgcccaat cctttctcag gcacggcgac gctgtccttt gttggcaaaa
    11401 attcctccac agcgtgcgtt acgctctaca atgccaaagg acagaaactg ggccaacgtg
    11461 actgtgccgt gattgccggc actaacgaga tcacgattga cccgcgaagc cttggcctgc
    11521 gcgaaagtgg tgtcttcttc ctcagtttga ggacaggtgg ccaggtcagc ctgctgaaag
    11581 gcttattcat caagtaatga tcatgtaagt gcagcctgac acaatgtccc gcaatagggg
    11641 gacacaagtt ctcgcttaaa gagctgtcag ccaattccag gcgggtgcac gctttccgtc
    11701 cttgttaaga aaaattaacc gaaaaagggg cgtttttcct tgccagaaaa cactttccgc
    11761 caaaactgtc ttttaggaag acaagcccat gagcaaacag atattcgtta ctgaagatga
    11821 gaccgacatc ctggaactgg tctgcatgaa gctcgaaaca gaaggataca gcaccctggg
    11881 attcacccgg gcgcaggacc tgctggacag attgcggcag gaattgccgg aactgatcct
    11941 gctggacctg atgctgcccg atttggacgg tttggaggcc tgccgccgca tcaagagcaa
    12001 tccagcctgg gaaaacgtcc ccatcatcat gctcactgcc cgcagcgaca ttgaggacaa
    12061 ggtgcgcggc ctggaattcg gagcggacga ctatatcacc aaaccctttg acagcaaaga
    12121 acttctggcc agggtaaagg ccgtcctccg ccgcagcgga tgggaaagtt cgcgcaacgt
    12181 gctcaacatc aacagcgatt tccggctcga ttttaaccgt tacgaagcct ggatccgcga
    12241 tcacagagtg gacctaaccc tcacggaatt caagattctg cagttgctca cacgccggcc
    12301 gggctgggtg ttcaaacgca gccaaatcct tgaccacctc tggggcaacg acaagattgt
    12361 gctggaacgc acggtggacg tccacatccg caatctgcgc gaaaaactgg gcgaccaagc
    12421 ctatatggta aaaaacattc gcggcgtcgg ctataagttt gagcccgacc aaagcagcgg
    12481 cagtgatgaa taaaacaagc cttggcaccc tgttgctgct ggctggagtc gtgatcctgg
    12541 cggctgtcgc tgtcctggcc cttctggacg tcgggccctg gctgctaata tcggtctccg
    12601 cgggggtcgc cggcattgcc ctgtacctcg tccatttctg gctaaaccgg gatctgcggc
    12661 atctttggaa acgcgcggaa gagctaaaac cccaagaacg caagctcagg tttggagagc
    12721 tcagaacctc tgaattgcac gatttggcgc agaaactgaa ccaggcgctg gcctctttcg
    12781 accaagcgat agggcatctg gcagttcacc gcacggaatt gcgattgctt ctgggcacac
    12841 tggaagaccc gctctggtcg caggatgacg acggaaatgt gctttgggcc aacgcgccgt
    12901 ttcacaagtt atttcccgcc tttgattcct cacgcgaaca gtcttaccgc gaaatcatcc
    12961 gcgatccgga gctgcaggaa gccatcttga gttccggaaa cgcgggggaa cagcttcccc
    13021 tttctctcaa catcgccggc cacagctaca tgctatccag cagccgcagt gtcagtaacc
    13081 ggcgctatgt ctttatcctg cacaacattg acgccatgga aagaacagcc cggatgaaga
    13141 aagacttcat cgtgaacctt gcccacgaac tgcgaacccc gctcacagcc atcaagggct
    13201 ttacggaggc gatgcaagat actccagggc aggatcatac ccgccaccag aaaataatcc
    13261 ttagccatac caaccgtctc atccatctga tccgcgacct cgaacagctt atccggctgg
    13321 aaagcatttc ccgtctggaa gcaagccaaa tcgagctgaa gccattcttt gccaatctca
    13381 gcctgatcct ggagccggac atccaagcca agggattgag cctgagcgtg gaactcgaag
    13441 aggggctgga ccggatggtc tgcgatcctt tccgctttga acagatattc atcaatctgg
    13501 tgcaaaactc tctccgttat acagatactg gcggtatcac aatctacgtc ggcaaagaag
    13561 gcgaaaacac agtcttcaca atctccgata ccggcagggg catccaaaat gaacatctgg
    13621 acagaatctt cgagcgtttc tacgtggcgg ataacagccg caaccgcgac ttcagcggca
    13681 ccggactcgg cttggccatc gtgaagcata tcgtggtgct gcatcatggc gagatttccg
    13741 tgagcagcga aatggggcgg ggcaccactt tcaggataag tattccgccg cttgcggagc
    13801 gtaagaatga atcaaatcaa ccaagctgaa gcaatggaat cctgttccaa cctccaagcg
    13861 gacagggact tactggtaaa cgagaacatc ggcctggctt acagcttcgc caaaaactgc
    13921 cagaaccgcg gcttaccgct ggaggacctg cgccaggaag ccctgctggg actgctggaa
    13981 gccgctgaac acttcgaacc agaacgggga ttgaagtttg gaacctatgc tggctactgg
    14041 ataaaaaaaa ggctgctggc cgcccttgag caggaaaaca aagccgtccg aaacacggag
    14101 ccgctcgggg aaagggattt ggctgatcac aaacagcagg tggatgcgcg aattgaatcc
    14161 agtatcactt ttccgtcagc catgcccgac ctcgagcgcg gcgttcttca actttccctg
    14221 aacgagggcc ttgccttgaa agaagtggcc tcccggctgg gcatttccgt ggagcgggtc
    14281 aagcaactgc gcggcaaagc cttgcggcgt ttacgctcat aagctgcttt tccctttttt
    14341 ggagctctat ttcacatcaa gtgggtaaag ccgaacgcca atatttgtaa aggctgtctg
    14401 tgcgcccctt ttttcctcgc ctaacaaacg cctgaatcca tcaggtttta actccttgct
    14461 gccttcgatg gcactttcct cgcccgacgc cagcgtttct tctggcgaca acgtttggcc
    14521 gttaaggcct tttctctggt tcctgacgtg cctcccgtat aatggccgca cttgatgcga
    14581 ggatcatgcg ggaggcgtgg gagggggata gcctcgggcg ttaaggacgt ggccggcaga
    14641 atatagcgat gagttagcat taatggctat ctttgatttg gctatcttta acttggctat
    14701 atttcaaatt gcctgggcaa tccgggggca aatgggttaa caaatcttaa cttcgtccat
    14761 ataaaatctt aacattcccc cggtattata ggggcagaac cttcaaagga gtaaaagatg
    14821 aagaaactgc ttctcgccac tctggtggga ttatttctgt tacctgcgct tcttggcgca
    14881 ttggagctta agatcagtgg ggaaatgtgg aaccgctgga ccctggaaca aggaaaggtg
    14941 ttcgccgata cttcgcttac ggcctacaaa aaagaaatca ccaaaaactt tttctcgctc
    15001 gagcgaggct atgtgggaat ggaagccaaa ttcagccaaa gcatgaaagg ccgcttcacc
    15061 gtggatatct tcagttcgga catcctcaag gacggggctg ggctgaagct gaaatatgcc
    15121 tacctggatt tttccaacct gatccccatc cctgacctga ccaccaccgt gggtctgcaa
    15181 aaagtttatt ttggaagtat ttacgactgg agctacaacc tgatcggcaa agcccccacc
    15241 gatgagtaca aatacgccaa ttccgccgac tacggcatca gcctgaacgg ttacctgcca
    15301 ggcggctggg gcgaatacca attgggcatg tacaacggcg agggctacaa aaactacggc
    15361 tccaacctta aagacaacat cgacccggct ttcctggcca acctgcgcat tgtacccact
    15421 gccggcctca caataggggg ttcggtaatg gccaacggca gcgagagaaa actcaagctg
    15481 gctgacgcca ccgaaaactc cagctacaac acccaattgc tcgccgatgg tattctgcgc
    15541 gcggcttttg gaccgctgga tatctgggcc gaatacctat acaaagacat aagctacgcc
    15601 ccgaatttca gcgccaaaga ctacagcgcc acctgcttca gcgtgtttcc caccctgaag
    15661 ctgaaaagcc tgatcggcgc cgacatcgat ctccagggac gctatgaccg ctctgacgaa
    15721 acccgcatgc cggacagtaa gaagaaaaac ctgctgaacg cctggacagg aggcctgaac
    15781 ttcaatttca tggccgacga cagcggcagc cccgccctgc aggcgcagct caactatgtg
    15841 agaaaagact atgacgaaga tgcttccggc accgactacg ccaatggcaa aaaggatacg
    15901 gacaccgtta tgctgcaact gaaatggcgc ttcgcttcta caatctacta aaggagaata
    15961 gaatgaataa aaaatatatt attgtcgccc tgatcctact tctggcaatt tcttttgccg
    16021 tagccaagtc ccgtaaaatc acctgttcag gttccaccac agtgcttccg atcgcccaag
    16081 ccaccgcgga agcctatatg gataagcatc ctgatgttaa tatctccatc cgcggcggcg
    16141 gatccggagt gggtattgcc gctctgcaaa acgggtctgc tgatattgcc aattccagcc
    16201 gcgtgatgaa ggccaaggaa atctcccagg ccaaatccaa aggcatcaac cccacgccgt
    16261 acgccgttgc gctggacggc atcgccatcg tggtgcacaa aagcaactcc gtggccaacc
    16321 tcagcatcgc ccagatccgt gacatctaca acggcaagat caagaactgg agcgaagtgg
    16381 gcggacccaa gctgccgatc gtcgtgattt cccgcgacgt ggcttccggt actttcgagg
    16441 tcttcaacga aaaagccctc ggcggcagca aagtggtttc cagcgcgcaa ctgctggcct
    16501 ccaacaacgc cgttgcctcc acggtgggca gcactcccgg cgggattggt tatataggcc
    16561 ttggctatct caccgaagag ataaaaaccg taaaagtgaa cagcattact cccaacacca
    16621 aaaccgtgca ggatggctcc tatcctctgg cccgcaaact ctatatgtac acgaacggca
    16681 aggcgaaagg cgatttgggc agctacatcg ctttcatcca atccgatgca ggacaaaaaa
    16741 tagtggtgga acagggtttc atcgctctta aatagttttg gcgagaaaac gaccaataaa
    16801 acctccattc acacgggagc ttcggctccc gttttgaggt aggaagaaat gaagactttt
    16861 aaggaaaaga gtttccagac aatcacctac acggcagcgt tgttcacggt ggcctgtttg
    16921 ctgggaatca tctttgggct tttccgggaa ggcttgccaa ttttcaaaca cgttggcctg
    16981 aaagagtttc tgttcaccaa caattggtat cccacccacg ctgaaccggg attcggagcc
    17041 ctagcgctga tcgtcgggtc cctggtcgtg accttgggtt cgctgatcat agccatcccg
    17101 ttgggactgg gagccgcgat tttcctctca gagatggccg ggccgcgctt gcgcgagatc
    17161 accaaacccg tgatcgaatt gctggcaggg atcccctccg tggtttacgg cctttttggc
    17221 atggcctttc tcgctcccct ggtgcgggag tggtttgatc tggacaccgg tttgaacgtg
    17281 ttcacggcct cgctgatcct gggtgtaatg atcgtgcctg tgatctgtag catgggcgaa
    17341 gacgcgctgt ccagcgttcc caaaaacctc cgcgaagctt cgctggccct gggcgccacg
    17401 cgagccgaaa ccattttcaa agctgtgatc cccgccgcaa aaaacggcct ggcaggaagt
    17461 gtgctgatgg gttttggacg cgccatcggc gaaactatgg tggtgctgat ggtggctggc
    17521 ggagcagccc gcatcccaag ttccatcttt gatccggtgc gtcccctcac ctcaaccatc
    17581 gccgccgaga tgggcgaaac cgtgatgggc gatttgcatt accagtccct gttcgctttg
    17641 gccatcatcc tcttcctgat cacttttgtt tccaatctca tcacggaatc catgctgctg
    17701 aagaggggca agaaatgaat aaacgaaaac ttatgaacag cttggtcacc ggcataatgg
    17761 cactgatgct ggttctcacc gctttgtttt tggtcttgtt cttggtgcgt atgttttccc
    17821 agggagccaa agtgttgagc tgggatttcc tcttcaccgc cccgcgggac gccatgagag
    17881 ccggaggtat ttatccagcc ctggtgggaa cttttctgct cactgcgctg tcgatggtgg
    17941 tggcgctgcc tttggggatt ctcaccgcca tttggctcac caattacggc agacctgttt
    18001 ggctggtcaa tattctccgg attgcgatca acaccctggc cggcacacct tccatcattt
    18061 tcgggctttt tggcatggca atgttttcca
//
LOCUS       DAOOWR010000003        16092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_3662_length_16092_cov_4.060547, whole genome shotgun sequence.
ACCESSION   DAOOWR010000003 DAOOWR010000000
VERSION     DAOOWR010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16092
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3662_length_16092_cov_4.060547"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLB85_00190"
     CDS             complement(<1..373)
                     /locus_tag="PLB85_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU71774.1"
                     /translation="MTARTLLSARGITKTYTMGEIQVHALRGIDLDVQAGEFIAIMGA
                     SGSGKTTFMNLIGCLDKPSSGTYILDGYDVHAMNDAEITQVRNRKIGYVFQSFYLLPR
                     TTALENVELPLLYSKNIHAKDR"
     gene            complement(376..1737)
                     /locus_tag="PLB85_00195"
     CDS             complement(376..1737)
                     /locus_tag="PLB85_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU71775.1"
                     /translation="MKKYLKYVIIALLLISAAIVFIQLRKSSNRPEWKIDSPSKGTIR
                     EIVTATGSLNPTVLVEVGTEVSGTIKTLYKDFNDKVRKGEVLARLDTEILAASVESAQ
                     AEVNKARVSRDEASMEYNNSETLFSRDMISSYELQKDKYSLDQAKLNLATALLRLQTA
                     EKNLKNATITSPIDGVIVSREVSEGQTVAASMNSPTLFTIANNLDQMEITASVDEADI
                     GKISVGLPVEFSVDAHAGQMFTGSVQQIRLKSETEQNVVSYSVIIDAANPDHKLLPGM
                     TTNVTIITQSKENVIRIPETATRFTPSKEVWELFGLKWEDDLIPNARKAAMEKAIASA
                     SGGRAPSRDNPRPDSAKAPSGRPPRGFPGGMPPGGMPGGVNRGGGNGRNGGSAMIWVL
                     KDKTPELIMVRTGVSDGAFVEVLSPLDPDTKIITGVNYKDPSQAKVNSALQQGRPGMG
                     PRF"
     gene            complement(1751..3001)
                     /locus_tag="PLB85_00200"
     CDS             complement(1751..3001)
                     /locus_tag="PLB85_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU71776.1"
                     /translation="MKAIWTLLLLLPLALLAQQYTLDELVEHGLVNSYGMLRGELAYE
                     TSRSNLSSATWNLLPEAGVSFGITNNFYHPNTPAASDLSSQAGFSISKTISLNDDAWF
                     NYRYAKLDAEKAELTRQSNASAYAYGVFTAYLDVLSTQRQLSSLQKNLEIQTRVWEQA
                     QVLNQLGKNTSFDLKESEIAVMNARISILQLENSVATKRAQLFSLARMDDAGYELAEL
                     APDMNYVLPQTSPEQSNQVKLLKADLKRGELGLTQNFLNYFPRVSLAYNFSRSVGGEN
                     FDFDTYGTNHTLGLNFSYSLWNQFKQGQTNKRGDLAQRLAELEIRQQIDEINQQYATL
                     SQELAYLTRLDELYSEKLAQSESQIRIAEERFRLGLIELLELDKTRVEFINSEIAYNN
                     NRYQILARQEAINNLLSQKIQGKW"
     gene            3228..4229
                     /gene="argF"
                     /locus_tag="PLB85_00205"
     CDS             3228..4229
                     /gene="argF"
                     /locus_tag="PLB85_00205"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOU71777.1"
                     /translation="MPCNLRNRHFLTLMDFSPQEIGFLLDLALNLKKAKYAGTEQQRL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHVTYLGPTGSQMGKKESVADTARVLGRMY
                     DGIEYRGFGQEIVEELAKYAGVPVWNGLTDQDHPTQVLADFLTAMEHLNKPLNEMTFV
                     YVGDGRNNVANALMIGGAKTGMDFRIVSPKALFPEASLLKKCKDVAKTTGATITITDD
                     IAKGVKNADVIYTDVWVSMGEPDSVWAERIKLLKPYQVNAAMMKKTGKPTTLFMHCLP
                     AFHDLKTTIGKQIHDKFKIRAMEVTDEVFESPQSVVFDEAENRMHTIKAVMVATLGS"
     gene            complement(4300..7191)
                     /locus_tag="PLB85_00210"
     CDS             complement(4300..7191)
                     /locus_tag="PLB85_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU71778.1"
                     /translation="MKRYLILAALLPLCLGLWANQYVAGIPVGLIRSPFQEESLNQAV
                     RRLSEEGCAILHYDADQAIALVPEGKYQDYRLAELDTAQKLYLVGKIPGQSRPELGAG
                     GRLLLELADAYLFESPLDEIQLREQITHPFTRLGPEPMRFSQTALPAASPRAARTDID
                     QMVALVNAASVQSTIQSLQDFQTRYARADNRLQVAQWIQQKFISYGVTDAVLQQFMWQ
                     NTIQYNVVATIPGTTYPNQYIVVGGHHDSISNNSDPYLFAPGADDNASGSVAALEMAR
                     VMMASGYQPRSSIRFVTFAAEEFGLWGSKHHAHTANLAGENIRLMINHDMIANQSGPQ
                     PWQVCLMPYDGSIDHSAYAALITEQYTDLDTYYGGLNSGSSDSHPFWQNGFHVIYFFE
                     DEFSPVYHTSQDVVANLNPAYCAEVIKASVACAASFADMPSSPVGLSAQDWGDGSTIL
                     LTWENLNDPLISYYNVYYSTVFGDWGAPLTTSGNYLSVQGLTQGQLYHFAVGSVDNFG
                     NESYLVYTTAMPLSVPLQPQNFIDQPLYQSILLDWDDNREHDFAGYRLYRSSQPDELG
                     QQIGGLLTESEYLDNDVVGSPSYYYYSLCAVDQDGNASPFTQVVKSRPVTLDQGVLIV
                     DETEDMSGTNPFMPTDQQADNFYAYASANFTTTELDINSLDENLRLADIGIYSSIIWH
                     GNDQASMDYPYFARDALNQYIQAGGNVFFSVYFPSLAFELNANYPGNFNSDSFIYDVI
                     GIASADYSGNAARFRFAIPAQGQIPAVSVDPEKTLSVMNGHIIRVESIGASPGCTTIY
                     NYGTDYADDTPQGAMAGMPVGVLNLNQSGKVCVVSFPLYNIYQDDARNLIDYVLTEYF
                     GETFSPAEDPGLAPAGGISISTNHPNPFKGETSFRLELKNNTRPVLVEIYNLRGQKVR
                     SLFVGYSPKSVVHSWDGLDDFGSAVSSGIYIIRTSQDGVAAQRRIALVK"
     gene            7367..8512
                     /locus_tag="PLB85_00215"
     CDS             7367..8512
                     /locus_tag="PLB85_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU71779.1"
                     /translation="MKRATLILAILLLALGALFAQSGDDNDMEMVTTQAPEQPVSVYF
                     GIYVDDLDYPTAHELGYKYLYGILVTGVSKGSPADKAGLKENDIMMEIDSKPVTNMEE
                     FDRQRSMMRPGQKINLRIWRNVEITDLELVLEPRPEGGKRFGSVISKDAYLEDPTDRP
                     SRKKDLGWGGGGWVPYYTILPMEHVNGLLNSIGDNETQNNDFGFNPISSKGIFMNGGA
                     GKGNIGKGFFLGGVGAGYEFSDVDPVTHASVRYEASFGGLTLDKRFLITDGFAASLGA
                     MFGAGGHTVYYTQTQSEFTWPQIFTENNFTATLKREYFIIQPRAELILNLIPWLSLRA
                     EVGYFCGIPTYNGWKVHSDAGDDITISGSPDTKFRGLSFSIGPWFGF"
     gene            8892..12104
                     /locus_tag="PLB85_00220"
     CDS             8892..12104
                     /locus_tag="PLB85_00220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU71780.1"
                     /translation="MARLFSKDYHEKSASWYFNHAVIALDRGDLQYWKSIDENGLYHL
                     HFIASGERRDFFATQLDIVYDPVSQTVASHHCHECRDEESCRHYLSVLRYAYLYLKTD
                     IFDSPAVETCDGSVLRGSEFWRGEHDRAVIEVERLYDPESDKARFYHSAYGRLDLPKL
                     LQIESGGRPEGISNRKLEEYRAQLEVLSDSELAFFRYLNAHKAAYSAKAKFWSLYKKD
                     LAPALVLMQDLDGRLTVRETGEPLKFNSQPYPLSLRVEPAGKKNYRVIPVILDELSVV
                     YPGFPTWLLFRNEARPVFLPLGNETLEQLFSHELLLSAKDLIYWRTVVNRELRKQDIY
                     LDFDPSIDLPQIVSGEPRVRLKVEKLGDNVALSGRLIYPSPRSGEEEQPLEIPLAVTC
                     FSAPLVRSDYIDAGGGGNAWFYLPPEIFNRVSQISARIPGSGQGRLERQSQLVFSDGE
                     ELTRLRQSLFELESEALDIEIAPELRGEFIQKVGLQVEIRARRSEEIDWFEYEVSYRY
                     RDLNFTHEELRRFFKSEEDFLHTADGRIIFISNPEVFRETERLLSRSEKLADEVYRSR
                     LVNLPYYQRYMLENPAFRMLGDEWLDSFSRDLVRGHLEGQMVLPTYLQTILRGYQKAG
                     VAWIRMLQHYRLGGILADEMGLGKTIQALALIHWAPEDSQSLVVCPKTLLYNWAAEIE
                     KFHTNIPYLIVEGGKAERLELLRDPNAKLFIISYTVVLNDIAALKEKEFEWIILDEAQ
                     NIKNVAAKRTYAIKKLSARHRLALTGTPVENNLTELWSIYDFLIPGYLGSLKRFKQEF
                     LEEPETGAVKLKRAVAPFLLRRTKKEVLLELPDKQEQVSWCRMHPVQEKLYLQIIDMV
                     QKKLLNAPESANLNFVHILAALMKLRQICNHPHLANPDILPELEASAKLEHLLELVTD
                     ALASGHKILVFSQFVKMLKIIRRVFDAQGLRYAYMDGHTKNRMDEVRRFETDPELKLF
                     LISLKTGGTGINLTSADTVILYDPWWNPMVENQAVDRTHRIGQTRKVQVFRLITKGSV
                     EEKIMHLQQHKLQLFDEVVSESQNVLSSLSREEISALFSY"
     gene            12592..>16092
                     /locus_tag="PLB85_00225"
     CDS             12592..>16092
                     /locus_tag="PLB85_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU71781.1"
                     /translation="MKKTFIMLIILGLAAFLAASQGSIKLGNFPTTAELTRSSGDGLS
                     VRYSIEKLDYQEVQTKEGVFTDLSVDQYTSTNNTGLPRLPLMRQIISVPEGAQVVPSF
                     TSLLRKSFILADEGVNYPVFPRQESVSKSADLTKIPFEVNRDFYNRDAWTTDAPISVT
                     ELGHMRGYRLFALDFVPVRYNPARGEIEVIYSAEVQVQFINPDHIATDELRARTYSPA
                     FEGPLRSVLLNPEPQRMSLNRYPMSYLIITPANFVNAMQPFVNWKKQEGFNVILATTA
                     ETGSTASAIKTYIQNIWNAATAENPAPTYLLIVGDTPQVPSNSGSTGSHVTDLTYVRL
                     QGSDYMPEMYFGRFSATTEAEVTNQVNKTLMHEMYTMPSDAYLGEVVMIAGADSYWAP
                     THANGQVNYGTTNYFNPTHGITSHTYLYPASQTSDAAIVANVSNGVGYVNYTAHGSET
                     SWADPTFTISDINNLQNVNKSAFVVGNCCLTSAFDIGICFAEAWLRAPNKGGVIYIGG
                     TNSTYWNEDYYWGVGFKTNPNGQAPAYDANKLGYYDALFHDHNEAFTDWGSTAASAMS
                     MGNMAVVQSNSSRINYYWEVYSVMGDPSLIPYLGIPAQNSFEAPQTMFLGLGTLDVVA
                     DPYSYVAISMNNELHGVGLTDASGMLTLNFTPFTEPGTAQIVLTRSLRKPMIANVQVV
                     PNSGPYVTVSPITVNDPNANGIAEAGETISLDLTFNNVGIQQASNLTATLSSTCEYVS
                     ILEATESLPDIPANGTTSQTDLFSVLIHNAIPDQTSVPFDITVSDGTNQWVTSRSITV
                     NAPHLQFGNPILFDPDGDGFLEPGEDISISVDIVNNGHMPTDSGTLTLVVNHPEVSLS
                     ATSFTLPPMAVGVTIPINFTASLSPQVQNGEMIPLGLSISAGAQNINHCILLTIGVTG
                     EGFESGDFSTYPWTNTSTVPWTIQSGSTNAHSGLYGAKSGAISHSSSTELSLTMNVGT
                     AGNISFWRKVSSENGWDFLRFSIDNEELGSWSGNQDWLQVSYPVSAGTHTFKWVYSKD
                     VSVSSGSDCAWIDDIVFPSAGSGEVAMIYVPQTEIKFLNVQPNDQLAADFVINNLGTV
                     PLSGTISYPSCMGLLHNGSAVANDSAYSIPVGQSHTYTLTYTVPNPAVNLDLEMIITS
                     NDTTTPAVIIPVRVEAGTANN"
BASE COUNT         3598 a         4674 c         4412 g         3408 t
ORIGIN      
        1 ggcggtcttt ggcgtggatg tttttggaat agagtagggg aagttccaca ttctccagcg
       61 ccgtggtgcg gggcaggagg taaaaactct ggaacacata accgatcttg cggttgcgca
      121 cctgagtgat ctcggcatca ttcatggcgt gaacgtcgta gccgtccagg atgtaggttc
      181 cggagctggg tttgtccaag cagccgatca ggttcatgaa ggtggtcttg cctgagccac
      241 tggcgcccat gatggcgatg aattcaccag cctgaacgtc cagatcgatc ccgcgcaggg
      301 cgtgaacctg gatttcgccc atggtgtagg ttttggtgat tccgcgcgcg ctgagcaggg
      361 ttcttgccgt catgctcaga accttggccc catgccgggc ctgccctgtt ggagggcgct
      421 gttaaccttg gcctgggagg ggtctttgta gttcacgccg gtgatgatct tcgtatccgg
      481 atcaaggggg ctgagcacct ccacaaaagc gccgtcggaa actccggtgc gcaccataat
      541 caattccggc gtcttgtctt tcagcaccca gatcatggcg gagccgccgt tgcggccatt
      601 gcctccgcca cgattcactc caccgggcat tccgccgggg ggcataccgc cggggaaccc
      661 acgtggagga cgtccggaag gggctttggc actgtccggc ctcggattgt cgcgggatgg
      721 agccctgccg cctgaagcgg aggctatcgc cttttccata gcggctttac gggcattggg
      781 aatcaggtca tcctcccatt tgaggccaaa aagctcccac acctccttgc tgggggtgaa
      841 gcgggtggcg gtttcaggga tgcggatcac gttttccttg gactgagtga tgatggtcac
      901 gttggttgtc ataccgggaa gcagcttgtg gtcggggttg gcggcatcga tgatcacgct
      961 gtagctgact acgttttgct cggtttcgga tttcagccgg atctgctgca cagagccggt
     1021 gaacatctgg ccggcatggg catccacgga gaattccacc ggcagaccca cggagatttt
     1081 gccgatgtca gcctcgtcca cactggccgt gatctccatc tgatccaagt tgttggcgat
     1141 ggtgaagagg gtgggcgagt tcatgctggc agccactgtc tgcccttccg aaacctcgcg
     1201 ggaaacgatc acgccgtcga tgggcgaggt gatggtggcg tttttgaggt tcttctcagc
     1261 ggtttgcagc cttagcaagg cggtggccag attgagtttg gcctggtcga ggctgtattt
     1321 gtctttctgc agttcatagg atgagatcat gtcgcggctg aagagagtct cgctattgtt
     1381 gtattccatg ctcgcttcgt cacgggaaac gcgggccttg ttcacctcag cctgggcgga
     1441 ttccacgctg gcggccagga tttctgtatc cagccgggcc agcacttcgc cttttctcac
     1501 cttgtcgttg aaatccttgt aaagggtttt gatggttccg gatacctcgg tgcccacctc
     1561 caccagaacg gtgggattga gggagccggt ggcggtaacg atttcacgga tggtgccttt
     1621 tgaggggctg tcaatcttcc attcgggtcg gttgctgctt ttgcggagct ggatgaaaac
     1681 gatcgccgcc gagatgagca gcagcgcgat gatgacgtat ttaaggtatt ttttcatggt
     1741 ataaccttta ttaccacttg ccctggatct tttgggaaag caggttgttg atggcttcct
     1801 gacgggcaag gatttggtag cggttgttgt tgtaggcgat ttcggaattg atgaattcca
     1861 cgcgggtctt gtccagttcc aaaagctcga tcaggcccag gcggaagcgt tcttcggcaa
     1921 tgcggatctg gctttcgctc tgagccagtt tttcgctgta aagctcgtcc aggcgggtca
     1981 ggtaggccag ttcctggctg agggtggcat actgctggtt gatctcatcg atctgctgac
     2041 gaatctccag ttcagccagg cgctgggcaa ggtcgccgcg tttattggtc tggccctgtt
     2101 tgaactggtt ccagagcgag tagctgaagt tcaggcccag ggtgtggttg gtgccgtagg
     2161 tatcgaagtc gaagttttct ccacccacgc tgcggctgaa gttgtaggcc aggctcacgc
     2221 ggggaaagta gttcaggaag ttctgggtga ggcccagttc cccacgtttg aggtcagcct
     2281 tgagcagttt cacctggttg ctctgttcag ggctggtctg aggcaggaca tagttcatat
     2341 ccggagccag ttctgccagt tcatagccgg cgtcgtccat ccgggcgagg ctgaagagct
     2401 gggcgcgctt ggtggcgacg gagttttcca gttgcaggat ggagatgcgg gcgttcatca
     2461 cggcaatttc gctttccttg aggtcgaagc tggtgttctt gcccagttgg ttcaacactt
     2521 gggcttgttc ccacacgcgg gtctgaattt ccaagttctt ctgcaacgat gaaagctggc
     2581 gctgggtgct caacacgtcc agataagctg tgaaaacgcc gtaggcatag gcgctggcgt
     2641 tgctttgacg ggtcagttca gccttctccg cgtccagctt ggcgtatctg tagttgaacc
     2701 aggcgtcatc gttgaggctg atcgttttgc tgatggaaaa tccggcctga ctactcaggt
     2761 cagaagccgc aggggtgttt ggatggtaga aattattggt gatgccaaaa ctcacgccag
     2821 cctctggcag caggttccaa gtggcgctgc tgaggttgga gcggctggtt tcgtaggcaa
     2881 gctcgccgcg cagcatgccg tagctattaa ccaagccgtg ctcaaccagt tcgtccaggg
     2941 tgtattgctg cgccagcagg gccagcggaa gcaacagcag gagggtccaa attgctttca
     3001 ttatcttcat atatccttca aaataaagat gtccagtggc atcaggcagg atagcctact
     3061 tattctttac ctatttcaga ggctttccct gcattattta ttgggtgaaa caatctctgg
     3121 atgcactttt tttcaaagcc gtgatacaga caaggaaaat ttcttgccgg gaaagctatg
     3181 ctaatttctt tggcatccaa agacaaaaat caagctaagg agaaattatg ccctgcaatc
     3241 ttagaaaccg ccattttctg accctgatgg acttttcgcc gcaggagatc ggatttctgc
     3301 tggacctcgc gctgaacctg aaaaaagcca aatacgcggg aaccgagcag caacgcctca
     3361 aaggcaaaaa catcgccctt atctttgaaa aggacagcac ccgcacccgc tgtgcctttg
     3421 aagtggccgc tctcgaccag ggcgcgcacg ttacatatct gggccccacc ggcagccaga
     3481 tgggcaaaaa agaatccgtc gcagacaccg cccgggttct tggccgcatg tatgatggaa
     3541 tcgaataccg cggcttcggc caagagatcg tggaagaact ggcaaaatac gccggcgtgc
     3601 ctgtctggaa cggccttacc gaccaagacc accccaccca ggtgctggct gatttcctca
     3661 ccgccatgga gcatctgaac aagccgctga acgaaatgac cttcgtttat gttggcgacg
     3721 gacgcaacaa cgtggccaac gcccttatga tcggcggcgc caaaactggg atggacttcc
     3781 gcatcgtgag ccccaaagcc ctgttcccgg aagcctctct gctgaagaaa tgcaaggatg
     3841 tggccaaaac caccggcgca accatcacca tcaccgacga catcgccaaa ggcgttaaaa
     3901 acgccgatgt gatctacacc gacgtctggg tctccatggg cgaaccggac agcgtttggg
     3961 ccgagcgcat caagctgctc aaaccctatc aggtgaatgc cgccatgatg aaaaagaccg
     4021 gtaagcccac cacccttttc atgcactgcc tgccggcttt tcacgacctc aagaccacca
     4081 tcggaaaaca gatccatgac aaattcaaaa tcagagccat ggaggtcacc gacgaagtgt
     4141 tcgaatcgcc ccagtccgtt gtcttcgacg aggcggaaaa ccgcatgcac acgatcaagg
     4201 ccgtgatggt tgccaccctc ggcagctgat cacctccacc atatagaaac aaagccggcg
     4261 gatcgctctg ccggctttgt ttattgatgg atggcaaatc tatttcacca gggcgatccg
     4321 gcgctgtgcc gcaactccat cctggctggt gcggatgatg taaatcccgc tggagacggc
     4381 gctgccaaaa tcgtccagac cgtcccagct atgcaccacg gatttggggc tgtagccaac
     4441 aaagagggat ctcactttct ggccgcggag gttgtaaatc tccaccagca caggccgagt
     4501 gttgtttttc agttccagcc ggaagctggt ttcgcctttg aaggggttgg gatggttcgt
     4561 cgagatacta atcccgccag caggcgccaa gccggggtct tcagcagggc tgaaggtttc
     4621 gccgaaatac tccgtcagca cataatcaat gaggttccgg gcgtcatcct ggtagatgtt
     4681 gtagagaggg aaactaacca cgcagacctt gccgctctgg ttcaggttca gcacccccac
     4741 cggcatgccg gccatcgctc cctggggcgt gtcgtcagcg taatctgtgc catagttgta
     4801 gatggtggtg caaccggggc tggcgccgat gctttcaacg cggatgatgt gtccgttcat
     4861 gactgacaag gtcttttccg ggtccacgct cacagcggga atctggcctt gagccgggat
     4921 ggcgaagcga aaacgggctg cgttgccgga gtagtcagca gacgcgatcc cgatcacgtc
     4981 gtagataaag gaatcgctgt tgaaattgcc cggatagttg gcattcagct cgaaagccag
     5041 cgaggggaaa tacacgctga aaaacacgtt gcccccggct tgaatgtact ggttcagggc
     5101 atcccgcgcg aaataaggat aatccatgct ggcctggtca ttcccgtgcc agatgatgga
     5161 tgagtagatt ccgatatcgg ccaggcgcag gttttcatcc agagaattga tgtcaagctc
     5221 tgtggtggta aagttcgcgc tggcataagc gtaaaaattg tccgcctgct gatcggtggg
     5281 catgaaggga ttcgttccgc tcatgtcctc ggtttcgtca acgatgagca caccctggtc
     5341 cagagtaacg ggacggcttt tcacaacctg ggtgaaagga ctggcattgc cgtcctggtc
     5401 caccgcgcaa aggctgtaat agtaatagct tggcgaaccc accacatcgt tgtccagata
     5461 ttcgctttcc gtgagcaaac cgccaatttg ctgtcccagc tcgtcaggct gtgaagagcg
     5521 gtagagcctg tagccggcga agtcatgctc gcggttatcg tcccagtcca gcaagatgct
     5581 ctgataaaga ggctggtcaa tgaagttttg cggctgcagc ggaacggaga gcggcatcgc
     5641 tgtggtatag accagatagc tttcgttgcc aaagttgtcc acggagccca ccgcgaaatg
     5701 atacaactgg ccctgagtga ggccctgtac cgacaagtag ttgccggaag tggtgagcgg
     5761 cgcgccccag tcgccaaaaa ccgtgctgta atagacattg tagtaactga tcaggggatc
     5821 gttcaagttt tcccaggtca gcaggatggt ggatccgtcg ccccaatcct gagcggaaag
     5881 gcctaccggg gaggaaggca tatcagcaaa ggaggcagcg caggccacgg aggccttgat
     5941 cacctcggcg cagtaagcgg gattgaggtt ggccacgacg tcctgggagg tgtgatacac
     6001 tggcgagaac tcatcctcga aaaagtagat gacgtgaaag ccgttttgcc agaagggatg
     6061 gctgtcgctg ctgccgctgt tcaagcctcc gtaataggta tccaggtctg tgtactgctc
     6121 cgtgatcagg gccgcgtagg cgctgtggtc aatcgatccg tcgtagggca tcaggcacac
     6181 ctgccagggc tggggcccgc tttggttggc gatcatgtca tgattgatca tcaggcggat
     6241 gttttcgccg gccagattgg ccgtgtgggc gtggtgtttt gagccccaga ggccgaattc
     6301 ctcggcggca aaggtcacaa agcggatcga gctgcggggc tggtagccgc tggccatcat
     6361 cacccgagcc atttccaggg cggccacgct gccgctggcg ttgtcatcgg ctccaggagc
     6421 gaaaaggtag gggtcgctgt tgttcgagat cgaatcgtga tgcccgccca caacgatgta
     6481 ctggttaggg taggtcgtcc ccggaatggt ggccacaacg ttgtattgga ttgtgttttg
     6541 ccacatgaat tgctgcagca ccgcgtctgt caccccgtag ctgatgaatt tctgctggat
     6601 ccactgcgcc acctggaggc ggttgtctgc ccgcgcgtag cgggtttgga agtcctgcag
     6661 gctctggatg gtgctctgca cggaagcggc gttcaccagg gcgaccatct ggtcaatgtc
     6721 cgtcctcgcg gcccgcgggg aggcggcagg caaagccgtc tgggagaagc gcataggctc
     6781 cgggcccagc cgggtgaagg gatgggtaat ctgttcccgc agttggattt cgtccagcgg
     6841 gctctcgaaa aggtaggcgt cggccagttc aagtagcagc cttcccccag cgccaagctc
     6901 aggccggctt tggccaggta tttttcccac cagatatagc ttttgggctg tatccagttc
     6961 agccagcctg taatcctggt atttgccttc cggcaccagg gcgatggcct ggtcagcgtc
     7021 gtaatgcagg atggcgcagc cctcttccga aagccggcgc acagcctggt tcaggctttc
     7081 ctcctggaac ggggagcgga tcagccccac cggaatcccg gccacgtatt ggtttgccca
     7141 aagccccagg caaagcggca acagcgccgc gaggatcaga tatctcttca tcgtatctcc
     7201 attatctcca cagcttgaaa gccacggaca ttcccagatt atgcaaagtc cgttccagtc
     7261 aaggtttttg ttccggattc ggcaattctg ttccggccac tatttttact tgtgatttac
     7321 cctgcctgcc ttattatttc ctttgaaaac actcaaggag tttcccatga aaagagcgac
     7381 gctcattttg gccatcctgc ttctcgcgct gggcgctttg ttcgcccaaa gcggcgatga
     7441 taacgatatg gaaatggtga ccacccaggc gcccgaacag ccggtttcgg tctatttcgg
     7501 aatctacgtg gacgacctgg attatcccac agcccacgaa ctcggctaca aataccttta
     7561 cggcatcctc gtgaccggag tctccaaagg atccccggcc gacaaggcgg gcctgaaaga
     7621 gaacgacatc atgatggaga tagacagcaa accggttacc aatatggagg aattcgaccg
     7681 ccagcgctca atgatgaggc ccggccagaa aatcaacctc cgcatttggc gaaacgtgga
     7741 aataacggac ctcgaactgg tgctggagcc gcgcccggaa gggggaaagc ggttcggcag
     7801 cgtgattagc aaagacgctt atcttgaaga ccccacagac aggcccagcc gcaaaaagga
     7861 cctcggctgg ggcggaggag gctgggtgcc ctactatacc atcctgccca tggaacacgt
     7921 gaacggcttg ctcaacagca tcggcgacaa cgaaacccag aataacgatt tcggattcaa
     7981 tcccatcagc agcaaaggta ttttcatgaa tggcggcgcc ggcaaaggca acatcggcaa
     8041 aggcttcttc ttaggcggag ttggcgccgg ctacgagttc agcgatgtcg atccagtcac
     8101 ccacgcctcc gtgagatacg aagcctcctt cggcggcctc accctggaca agcgtttcct
     8161 gatcaccgac ggtttcgctg cctcgctggg cgcgatgttt ggagcgggtg gacacactgt
     8221 ctattatacc cagacccaat ctgagttcac ctggccccaa atcttcaccg aaaacaattt
     8281 caccgccacc ctcaagcgcg aatacttcat catccagccc cgggcggaac tgatcctcaa
     8341 cctcattccc tggctctcac tccgcgccga agtgggctat ttctgcggaa tccccaccta
     8401 caatggctgg aaggtccatt ccgatgccgg cgacgatatc accatctccg gctcgcccga
     8461 taccaaattc cgcggcctca gcttcagcat cggcccctgg ttcggtttct aaccgctgtt
     8521 tgataatcat ccaaatcccg gctgcctctg gctccgggat tcttttttgc cgacagtcag
     8581 gccctttgcc cctgcccttc cgcctccctt tcgcctcccg tagagttccc gtagctcata
     8641 cgggaagtca gcgggaagtg aatgggatgt gaattggaag gggcaaacgg cgaagctagc
     8701 gggcagccca gcaaaacccg gaaaagcccg gtttgccagt tctataatag tgggtaacgg
     8761 atcgcggggc ggcagaagaa acaggaaaat tggcctgaaa cttgcagaaa aaaggaaatc
     8821 agagcggaaa taagttgaca aaaatggact tccgcttgcc gatggactga ttaaagcacc
     8881 agagaggaaa gatggcccgc ctgttcagca aggattacca tgaaaaatcg gcttcgtggt
     8941 atttcaacca tgccgtgatc gcccttgacc ggggcgacct gcaatactgg aaatccatcg
     9001 acgagaacgg cctgtatcat ctgcatttca tcgcttccgg ggagcgccgc gattttttcg
     9061 ccacccagct tgacatcgtt tacgaccccg tttcccaaac cgtggccagc caccactgcc
     9121 acgagtgccg ggatgaggaa agctgccgcc actacctctc ggtgctgcgt tacgcctatc
     9181 tttacctgaa aaccgacatc ttcgacagcc ccgccgtgga aacctgcgac ggcagtgtgc
     9241 tgaggggcag tgagttctgg cggggggagc atgaccgcgc cgtgatcgag gtggagcggc
     9301 tttatgaccc ggaatccgac aaagcgcgct tctaccattc cgcctacggt cggctggacc
     9361 tgccgaagct gctgcaaata gagagcggag ggcgtcccga gggaatttcc aaccggaaac
     9421 tggaggaata ccgggcccaa cttgaggtgc tgtccgattc cgagctggct ttcttccgct
     9481 atctgaacgc gcacaaggcc gcctacagcg ccaaggcgaa attctggtcg ctctataaaa
     9541 aagacctggc cccggccctg gtgctgatgc aggatttgga tggacggctg acggtgcgcg
     9601 agaccgggga accgctaaaa ttcaactccc agccctatcc cctcagcctg agggtggagc
     9661 cggccggcaa gaaaaactac cgcgtcatcc cggttatcct ggatgaactt tcagtggtct
     9721 atcccggctt tcccacctgg ctgcttttcc gcaacgaggc acgccccgtc tttctgcctc
     9781 tgggcaacga aaccctggag caactattca gccatgaact gctgctcagc gccaaggacc
     9841 tgatctactg gcgcaccgtg gtcaaccgcg aactgcgcaa acaggacatt tacctggatt
     9901 tcgacccctc catcgacctc cctcagatag tttccggcga accgcgcgtc cgcctgaaag
     9961 tggaaaagtt gggcgacaat gttgccctct ccggccggtt gatctatccc tcgccgcgct
    10021 ctggggagga ggaacagcct ttggagatac cgctggctgt gacctgcttc tcggcgccat
    10081 tggtgcgcag cgactatatt gacgccggtg ggggcggcaa cgcctggttt tacctgccgc
    10141 cggagatttt caaccgggtg agccagatct cggccagaat ccccgggtcg gggcagggcc
    10201 gcctggaacg ccagtcccaa ctggttttca gcgacggaga agaactaacc aggctgcgtc
    10261 agagcctctt tgaactggaa tctgaggccc tggacatcga aatcgcaccg gaactgcggg
    10321 gcgaattcat ccagaaagtg gggctgcagg tggaaatccg ggccaggcgc agcgaagaga
    10381 tcgactggtt cgaatatgaa gtttcctacc gctaccggga cctaaacttt acccatgagg
    10441 aactgaggcg cttcttcaaa tctgaagagg atttcctcca taccgctgac ggacgcatca
    10501 tcttcatttc caatcctgag gtcttccgcg aaaccgagcg tctgctgtcg cgcagcgaaa
    10561 aactggcgga cgaggtttac cgctcccggc tggtgaacct gccctattac cagcgctaca
    10621 tgctggaaaa cccggctttc cgcatgttgg gcgacgaatg gctggacagc ttttcccgcg
    10681 acctcgtgcg cggccatctt gaaggccaga tggttcttcc aacttatctg caaaccatcc
    10741 tccgcggcta ccagaaagcg ggagtggcct ggatccggat gctgcagcac taccgcctgg
    10801 ggggaatcct cgccgacgaa atgggcctgg gaaaaaccat ccaggcgctg gccctcatcc
    10861 actgggcccc ggaggacagc caaagcctgg tggtctgtcc caaaacgctg ctctacaact
    10921 gggcggcgga gatagagaag ttccacacca atatccccta cctgatcgtg gaaggcggca
    10981 aggccgaacg gctggagctg ctgcgcgacc cgaacgcgaa gctcttcatc atctcttaca
    11041 cggtggtgct gaacgacatc gcggccctca aggaaaaaga atttgaatgg ataatcctgg
    11101 acgaggcgca gaacatcaaa aacgttgccg ccaaacgcac ctacgccatc aaaaagctct
    11161 ccgcccgcca cagactggcc ctcaccggca ccccggtgga aaacaacctc accgaactct
    11221 ggtctatcta cgacttcctc atcccgggtt atctgggcag cctcaagcgc ttcaaacagg
    11281 aattcctgga agagcccgaa acaggcgccg tgaagctcaa acgcgcggtg gcgcccttcc
    11341 tgctgcgccg caccaagaaa gaggtgctgc tggaacttcc ggacaaacag gaacaagttt
    11401 cctggtgccg gatgcacccc gtgcaggaaa agctctacct ccaaatcatc gacatggtgc
    11461 agaaaaagct gctcaacgcc ccggagagcg ccaacctcaa cttcgtgcac atcctggccg
    11521 cgctgatgaa gctgcgccag atctgcaacc acccccacct ggccaacccg gacatcctgc
    11581 cggaactgga agcctcagcc aaactggaac accttttgga actggttaca gacgccctgg
    11641 caagcggaca caagatcctg gttttctccc agttcgtgaa gatgctgaag atcatccgcc
    11701 gcgtcttcga tgcccagggt ctgcgctatg cctacatgga cggccatacc aaaaaccgca
    11761 tggacgaggt gaggcgcttt gagacagacc cggagctcaa actcttcctc atcagcctca
    11821 aaaccggcgg caccggcatc aacctcacct ccgccgatac cgttatcctc tacgacccct
    11881 ggtggaaccc aatggttgaa aaccaggcgg tggaccgcac ccaccgcatt ggccagaccc
    11941 gcaaggtgca ggtctttcgc ctgatcacca aaggaagcgt ggaggaaaag ataatgcacc
    12001 tgcaacagca caaactgcag ctcttcgacg aggtggtttc cgaatcccag aacgtgctct
    12061 cctccctcag ccgagaggaa atcagtgcgc tattcagcta ttgagcggac atataagttt
    12121 tggcccatgt ttaaaaaaga gtgggtcgct cagttcgaag agagcttttt tctcatctgc
    12181 cggagagagg gattttccga ttcaatcaat tctatcaagg cctggtagcg accataacgg
    12241 tcggggtgaa aggactgaac catccgctgg aacagagcaa cgcaggagta tccaccggca
    12301 aaaaatccct ctcttcagtt aatcctgtgc cggatggcaa aagaaagaaa ttgacagatt
    12361 ccttgggtcc aatgaattcg atagattaat ttgaatgatg tggaagagat tcgtcccaca
    12421 aacggaaact gtggcgctta caggtcctat cattcggtgg agcaaggtgt tgcgcggcac
    12481 gccagatgac aggctcaaaa ctttagcctg tttcggaact gaacttgcat catgatgcca
    12541 tatattaagg aatacaagtt taaaacattg atacaaagct aaggagagaa catgaagaaa
    12601 actttcatca tgctcataat cctgggattg gcggcgtttc tggccgcgtc ccaaggatcg
    12661 atcaaactgg gaaactttcc caccactgct gagcttaccc gcagcagcgg ggacggcctg
    12721 agcgtccgct acagcattga aaaactggac taccaggaag ttcagaccaa ggaaggtgtt
    12781 tttacagacc tcagtgtgga ccaatacact tccaccaata acaccgggct gcccaggctg
    12841 cccctgatgc gccagatcat cagcgttccg gaaggagcgc aggtggtccc aagcttcacc
    12901 agcttgttga ggaagagctt catactggcg gatgagggtg tgaactatcc cgtttttcca
    12961 cgccaggaat ccgtttccaa aagtgctgac ctgaccaaga ttccctttga agtgaaccga
    13021 gatttttata accgcgatgc ctggaccacc gacgccccga tcagcgtgac cgaattgggc
    13081 cacatgcgcg gttacagact gttcgctctc gattttgtgc ccgtgcgcta caaccccgcc
    13141 cggggcgaaa tcgaggtgat ctacagcgcc gaagtgcaag tccagttcat caatccggac
    13201 cacatcgcca ccgatgagct gcgcgcccgc acctattccc ctgcgtttga gggccccctg
    13261 cgcagcgtat tgctcaatcc cgaaccccaa agaatgtctt tgaaccgcta tccgatgagc
    13321 tatctgatca tcacccctgc caacttcgtg aacgccatgc agccttttgt gaactggaaa
    13381 aagcaggagg gattcaacgt tatcctggcc accaccgccg agaccggcag cacagccagc
    13441 gccatcaaaa cctatatcca gaacatctgg aacgcagcca ccgccgaaaa cccggccccc
    13501 acctacctgc tgatcgtggg cgatacgcct caggtgccgt ccaattccgg ctccactggc
    13561 agccacgtga ccgacctcac ctatgtgcgt ctgcagggct cggattatat gcccgagatg
    13621 tattttggcc gcttttccgc caccaccgag gccgaggtga ctaaccaggt gaacaagacc
    13681 ctcatgcacg aaatgtacac catgcccagc gacgcttatc tgggagaagt ggtgatgatc
    13741 gccggcgcag acagctactg ggctcccacc catgccaatg gccaggtcaa ctatggcacc
    13801 accaactatt tcaaccccac ccacggaata acctcccaca cctatcttta cccggcttcc
    13861 caaacctccg acgcagccat cgtggcgaat gtttccaacg gcgtgggcta tgtcaactac
    13921 acagcccacg gcagcgagac ctcctgggcc gaccccactt tcaccatctc cgatatcaac
    13981 aacttgcaga atgtgaataa aagcgctttc gtggtgggca actgctgcct caccagcgcg
    14041 ttcgacatcg gcatctgctt cgccgaagca tggctgcgtg ccccgaacaa gggcggtgtg
    14101 atctatatcg gcggtaccaa cagcacctat tggaatgaag actactattg gggcgtgggc
    14161 ttcaaaacca atccgaacgg gcaagccccg gcttacgacg ccaacaaact gggctattat
    14221 gacgccttgt tccacgacca caacgaagcc ttcacggatt ggggcagcac cgccgcctcc
    14281 gccatgtcaa tgggcaacat ggccgtggtg cagtccaaca gcagccgcat caactattac
    14341 tgggaagtgt actccgtgat gggtgatccc agcctcattc cctatctggg gattcccgcc
    14401 caaaacagct ttgaggctcc gcaaacgatg ttcctgggcc tcggcacttt ggatgtggtc
    14461 gccgatccct acagctatgt ggccatctcc atgaacaacg agctgcacgg cgtaggactt
    14521 accgatgcct ccggaatgtt aaccttgaac ttcaccccct tcaccgaacc cggcaccgca
    14581 cagatcgtgc tcacgcgctc cctgcgcaag ccgatgatag ctaacgtgca ggtggttccg
    14641 aacagtggtc cctacgtaac cgtaagcccc atcaccgtta acgaccccaa cgccaacggc
    14701 atcgccgaag ctggcgagac catcagcctg gaccttactt tcaacaacgt gggcatccag
    14761 caggcctcca acctcaccgc caccctcagc agcacctgcg agtacgtgag catcctagaa
    14821 gccactgaaa gcctgcccga cattcccgcc aacgggacca ccagccagac agacctgttc
    14881 agcgtgctga tccataatgc cattccggac cagacaagcg ttccgttcga cataactgtt
    14941 tcagacggaa ccaaccagtg ggtcaccagc cgcagcatca ctgtgaacgc tccccacctg
    15001 caattcggca atcccatcct cttcgacccg gacggcgatg gcttcctgga gccaggagaa
    15061 gacatctcca tcagtgtgga tatagttaac aacggccaca tgcccaccga cagcggcacc
    15121 ctcaccctcg ttgtaaacca tcccgaggtt agcctcagcg ccaccagctt caccctgccg
    15181 cccatggcag tgggtgtgac aattcccatc aacttcaccg cctccctgag cccgcaggtg
    15241 caaaacggcg aaatgattcc gctcggcctt tccatctccg ccggcgcaca gaacatcaac
    15301 cactgcatcc tgctcaccat cggcgtaaca ggcgaaggct tcgaaagcgg cgatttcagc
    15361 acctatccct ggacaaacac cagcaccgtg ccctggacca tccagtccgg ctctacaaac
    15421 gcccacagcg gcctctacgg agccaaatcc ggcgcaatct cccacagcag cagcaccgaa
    15481 ctatccctca ccatgaacgt tggtaccgct ggtaacatca gcttctggcg taaggtttcc
    15541 tccgaaaatg gctgggattt cctccgtttc agcatagaca atgaagagct cggttcctgg
    15601 agtggaaacc aagactggtt gcaagtgagt tatcctgttt ccgctggcac ccataccttc
    15661 aaatgggtct atagcaagga tgtttccgtg agttccggca gcgactgcgc ctggatcgac
    15721 gacatcgtat tccccagcgc cggcagcggt gaagtggcca tgatctatgt tccccagacc
    15781 gagatcaagt tcctgaatgt tcagcccaat gaccaactcg cggctgactt cgtgatcaac
    15841 aaccttggca cagtgcccct gagcgggacc atcagctatc catcctgcat gggcctgttg
    15901 cacaatggca gcgctgtggc aaacgattcc gcctacagca tccccgtggg ccaaagccac
    15961 acctacacgc tcacctacac agtgcccaat cccgctgtca acctcgattt ggagatgata
    16021 atcacttcca acgacaccac cacccccgct gtgatcatcc ccgtgcgcgt tgaggctggt
    16081 acagccaata ac
//
LOCUS       DAOOWR010000004        15601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_3789_length_15601_cov_4.199730, whole genome shotgun sequence.
ACCESSION   DAOOWR010000004 DAOOWR010000000
VERSION     DAOOWR010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_15601_cov_4.199730"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..910
                     /locus_tag="PLB85_00230"
     CDS             344..910
                     /locus_tag="PLB85_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU71782.1"
                     /translation="MKPRPEFSHPVHEVIRKRWSPRFFADRPVSEEQVLTLFEAARWA
                     ASAMNEQPWRFIWSLKDGSEKYKKLFSCLYEGNQIWASSAPLLILTLAKTTFSFNGKP
                     NPWSSHDLGLAVGNLTTQASLMDLFVHNMGGFDRQKAREIFSLTEDLEPITMIAVGWA
                     EDPENLSEQERQREYLMQKRRSLQDIFL"
     gene            1054..1611
                     /locus_tag="PLB85_00235"
     CDS             1054..1611
                     /locus_tag="PLB85_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71783.1"
                     /translation="MIPSKKPCVLLLALLLASLCAAQEQPDFSFQLGRYGTLGFGYGL
                     PYGGYGFSADMYIFDQLALTVDVGTLLYAAGYELGIKYLHGNAGKLWRPQAILLYGVN
                     GIIPYNFSNTEVVQTEVYNGFTAGLGSQFMFGKKRRHGLDFGVTYVISSGLFKRLEEL
                     EGYEFGASSRFGFYLGYRYAFQFLY"
     gene            1717..2325
                     /locus_tag="PLB85_00240"
     CDS             1717..2325
                     /locus_tag="PLB85_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71784.1"
                     /translation="MLEKQTHPYTPYFPKTAEAVIIGSAPPWRFCTGRAENLRAGDVD
                     FFYGSVDNRFWRVMLEVLGTEPGPGTAELLKTILKNHKLGMGDILKSFTRRDRKSSDS
                     DLSEFSYDFSLVEGIMDRASIRKLFFTSEFAYRHFKRALREQGYKLKQTAIARRQYSL
                     SVSKPGFKRDFEISVLLSPSRRGRHSFEDLVANYKEQFSQFF"
     gene            complement(2398..3729)
                     /locus_tag="PLB85_00245"
     CDS             complement(2398..3729)
                     /locus_tag="PLB85_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOU71785.1"
                     /translation="MNSAWYHSNPALYYLRGSLRGLPFEICLREFSIAEPLFDILTQI
                     VRDRPDVASFSAYVWNSLDLRRLIPELKKLMPGLKVVLGGPEATNGDFGLAEGDFRVN
                     GPGEGVFRRLAESGFEFPGGTYEAPAPPLSELPFLYRRSDRPALQGRLVYYESSRGCP
                     FRCAYCLSALDKRNEARFDPSLASDRRKLRSELDRLLELKPRTLKFVDRSFNAHPRLA
                     RLIWEHAIRQKQSCEFHFEIYPDLLTEEDLRILEKAPPGRIRFEVGVQTVNAAVSRAC
                     GRNSNWGNVKAALNALRERTAICVHADLLVGLPGEKYASVLRSLDELASTFPAEIQLG
                     MLKILPGTPMQEIASQRGFLWLNDPPWQVLKTDALSFEQVARLQELAKILNLYWNKGE
                     FTAQWQELLSAGNRASSICLRLLKLHRQRGLTLHSVGKSTRAEVFAELLSL"
     gene            complement(3755..3988)
                     /locus_tag="PLB85_00250"
     CDS             complement(3755..3988)
                     /locus_tag="PLB85_00250"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOU71786.1"
                     /translation="MPPGMELLVVPANDYAVFEHRGALDNLCQTYELVYSEWLPASAL
                     ERVGNLDFELYDWRFRWGQPDSVLEIWVPVRQK"
     gene            complement(3948..4226)
                     /locus_tag="PLB85_00255"
     CDS             complement(3948..4226)
                     /locus_tag="PLB85_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71787.1"
                     /translation="MKPRLVSREAFTVVGFKCRASKQNNVVPRLWDDFNKVCAGIPHS
                     VKDRTAYGVCYLGGRRSLRRQVFLSGRGRGFNRQRHSPGHGTACGSGK"
     gene            complement(4538..4969)
                     /gene="smpB"
                     /locus_tag="PLB85_00260"
     CDS             complement(4538..4969)
                     /gene="smpB"
                     /locus_tag="PLB85_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU71788.1"
                     /translation="MMLKNRRALHEYFVIQKFEAGIALVGSEIKSIRAGKVNFKDSYA
                     RVTDGECWLLNLHISPFEKSTHFSPDPTRKRKLLLHKHEIRRLRAKTEEQGMTIVPLE
                     IFINEKGLCKVTIALVKGKKTYDKRESLQRKDLERDRDREG"
     gene            complement(4971..6284)
                     /locus_tag="PLB85_00265"
     CDS             complement(4971..6284)
                     /locus_tag="PLB85_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU71789.1"
                     /translation="MNKSIDLLLQGGTILTLDSGLRVLEGQAIAIDQGQILDIFDPRE
                     KSYQARRTLDTSNCIVMPGLINAHTHLPMSYFRGLADDLPLQTWLHDFIWPLEAKMLK
                     SDFISQAALHGAAEMIKNGITQIHDMYFDMPAVANACSQAGLRAIIGEAVLEGNSGTR
                     EAPLGSKVLELRQRYADDPLLDFNLAPHSIYACSEKTLRECAEAAARHGILLHTHLAE
                     TRGEVEDCLKAHSLKPVPYLQKVGILELPALYAHGVWTDPDEIALLAANPASVAICSD
                     SNLKLASGILPLAQYLESGVNLCFATDGVASNNNLDLLAEMDLTAKLHKAVNNDPSFL
                     PAVQTVQMATIGGAKALGVADKRGSLEPGKDADICVLELGGIESQPLYNPWSHVVYTL
                     GSKQVRDVVIAGEVVLEGGRLCKVDEAELIANAKMWRQRVLEELG"
     gene            complement(6291..7325)
                     /gene="mtnA"
                     /locus_tag="PLB85_00270"
     CDS             complement(6291..7325)
                     /gene="mtnA"
                     /locus_tag="PLB85_00270"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU71790.1"
                     /translation="MIVDGREYRSVWWEHGRLRMIDQNRIPHDFTIMDFSDHLQVAEA
                     IRNMNVRGAPAIGAAGAFGLALAAKNAPDERFRSHIRKAHDLLLATRPTAVDLQSGVN
                     YVYERTIKFIPDLAHARKVALLAAKEFANRSAEDCRLIGEQGLHLIPDGARILTHCNA
                     GALATVDWGTALAVIRLAHRKGRDIFVYVSETRPRHQGSKITAWELEQEGIPHAIIPD
                     SASGYYFWKKEIDLVMTGADRVCLNGDIANKIGTYDKAVLAKYHGIPFYIAAPLSTFD
                     FACRQGTEIPIEFRDEEEIKLYGGQITANPGSPVLNPGFDITPAELIQGIITPKGVFA
                     AAEAAQKVQG"
     gene            complement(7315..9957)
                     /locus_tag="PLB85_00275"
     CDS             complement(7315..9957)
                     /locus_tag="PLB85_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU71791.1"
                     /translation="MAKKNVNPKIQHKGVKSVKQAARAHSSARTPRQAPAAAPGWLEV
                     LSAKPWLPWALMAFLLVFVSLLYFPVAFGGKTPHASDISQWQGAANKIIEYNQNHADN
                     ALWTQSMFSGMPSYMISFPNRWPFLENLSRLTDKLINWRIFLLFIGSLGMFLLLRFLK
                     LDVWTSFFGAVAFMFSCHWLGLVEIGHNTKFRAIMYIPWVIWAFMRLRQKPGLLSLGF
                     LATTLIMQLRENHPQISYYLYLFLGLYWLWQLVESIRAKDHKSFWLFTLLAVVAVGLT
                     LLAVMNPYLSTMEYSKFTQRGGSGGLETSYAQGWSFHPKEIITFFIPDFYGGVSPNYW
                     GYMPFTQVYNYFGLIVLAFGIIALIGSKHRRTAVFLSISSLIFLIMSFGSATPRLSDL
                     FLKYLPYFNKFRVPSMILTMVQINAVLQAALGVDTILSLSAEQSKDWSKGLLKAALIC
                     GGVFFLWLIAAKALFGGLGFASASDLQQLSQHGLTELPGTVKETRLGLLYNSGLISLL
                     LLSLSLALAWLKSAKKLPQVAFVLLLTLLAFIDLWVYTGKFLKSENLQARQEYTDRFA
                     PQDYDEFLLADKSNYRILPLGQSMLSEARMGKPAGEFAYHHQTVTGYSAAKLERYDKL
                     LKLIEGDPATQKPGEWGRLMMGMYGMKEGEMPVDKPTPVMDMLSTKYILHPDPLPNDS
                     LFTLDFPPYNQFYNKLTPVFTGFDGTSVYRNETALPRAWFVDSVQKVAPADSILPLLR
                     SESFDPRRLAFVETDLKDIQKPDSARVTQTTAEMHKLAYDVYTDKPAFLVLSEVYYPA
                     GWKATLDGKEIPIHAANYILRGLQIPAGAHKLELTFAPESYKTSVRLSFIGLLASLLA
                     LGGGIAYTRFKNRPIPVEPTSDDS"
     gene            10056..10466
                     /locus_tag="PLB85_00280"
     CDS             10056..10466
                     /locus_tag="PLB85_00280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOU71792.1"
                     /translation="MVFEFRKRIYGFECDVYGHLNNANYLQLLESARSEAMIEMGMSM
                     SRMHELDIQVFIRGFELDYRQSIEHEDLITIKSWFDEMNRVKGHWVQRIYNSRGELCF
                     EAKMVAVFASGGKATRVSEELLEIFLGYLEPPAD"
     gene            10753..12372
                     /locus_tag="PLB85_00285"
     CDS             10753..12372
                     /locus_tag="PLB85_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU71793.1"
                     /translation="MQFIQLKDIHYTYPDQYQPILAGVSLVVAAGEKIALIGKNGCGK
                     TTLLRIILGELSPTQGSISYPSSSPTMSYLPQDIRVSAPLSVRDFLLRVRPRQFELLT
                     RIESLTSTENLSSSAGLELASLWQEYNDQNTAEWEKEVHAILLGMDLLPLAERPCATL
                     SGGESTRLQLAALLLEKPDILILDEPTNHLDSEQLLWFEDWMLDYGGAVLYVSHDRVF
                     IDRTATKIAELEAGRMELRAGNYQSFVRDRQQLKDHQMVQYRERQRLLRQLRDASRKR
                     RAWASSFQKETSGEGGGYVFEMISNPARTQMQQARHIENRIKMLTERYPVEKPHQDKL
                     RHLAFEEVQVGDRELISVAGMGFRYQENWIFRDFYLHLYGAEKLWLAGPNGSGKTTLL
                     HLLLGSLEPCEGTISRANRLRIGYYEQDLSKLDPQAVVLDFLKTSGKEESQIRTLMGC
                     IGLKTDLAFARTGSLSWGERAKLQLLMLLLGEYNVLLLDEPTNHLDIRSREMLEESLE
                     NFNGAVVFVSHDRAFINRLATRKVELANAPA"
     gene            complement(13036..13239)
                     /locus_tag="PLB85_00290"
     CDS             complement(13036..13239)
                     /locus_tag="PLB85_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71794.1"
                     /translation="MQKIISLILLAALLIGMLTLAACQPPQDKTDANEESTTQETNSD
                     EPASDTELISTEGDTTGGAQSQN"
     gene            complement(13364..13549)
                     /locus_tag="PLB85_00295"
     CDS             complement(13364..13549)
                     /locus_tag="PLB85_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71795.1"
                     /translation="MKKLALIALLAMMLIGMLAFSACKAEEAEEVVEETTEEVVDTTV
                     APIVDTLAQQAPVNGVN"
     gene            complement(13762..15426)
                     /locus_tag="PLB85_00300"
     CDS             complement(13762..15426)
                     /locus_tag="PLB85_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU71796.1"
                     /translation="MKRALLAAALLLAWVLAPALSVDKVSFSANFPFDETALLKASGL
                     TAGSEYTPADVNAAIALMQAWLQANGHPFVKIANPELVPLSESSLELAFKLTEVLPAQ
                     DCELRFRGLRYFSEAKLRELLLLVDKSKVGLSELPRTMDRILDEYHRRGYLFASVGLD
                     SLTLGENLAAYIGIEEGKPLKPEKYYFQGNKYTRDQTLIRLAGFSSGQVVTPEAIRAA
                     EDRILGKSYIEACLIEPVDPASLLIKIEEGKMTSLEGVLGFTRVGAKNEFTGFLNLGF
                     LNLWGSDRSLTLNWRKLPLTSLLRFSYHESGPNVFPLSGDLRLSREEEKDSFIKSAVG
                     ADIYSYYDVHRYGLELAVESVNYYVPAAKDSLLVETTSSRGIGAFWRLDSRDRAFNPS
                     KGMQTNITYRLHQSKRSGWNNALEADHTQYIGLAPRWTCALGVHLRSLADSSSVAYET
                     YRLGGYNSLRGYREDEFSSWRLGWASLELRYLISSQARIYLFYDHGLLARDKNSLRAD
                     LLAPGLGIKLRTRLGILSIEYALGYRENGFADFSAGMVHAGLESNF"
     gene            complement(15437..15598)
                     /locus_tag="PLB85_00305"
     CDS             complement(15437..15598)
                     /locus_tag="PLB85_00305"
                     /inference="COORDINATES: protein motif:HMM:NF018161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71797.1"
                     /translation="MRKDVLAKCYGGDVSRKRKLLEKQKEGKKKMKEIGSVTVPQEAF
                     LAVLKADRD"
BASE COUNT         3288 a         4206 c         4576 g         3531 t
ORIGIN      
        1 cgattaacgt gcctgccaga atggcggacc cgcaacctcc cagaacctac cagaaagtgc
       61 cgaaaaactc tgcgcccatg cgtcttgcga tgttaggcat gattcgctcc ttgctgttgt
      121 tttgccgccg gacacactgc ccgcgttaac gggctgttat ttatgacgcg tggccgtcct
      181 gcggctccag cccagtttgc ttcatcttgc ctgtgagtca agttttttct gaaccctggc
      241 cagaagcggt gtcgggacat gccatttcgg gtggattctt gccttgatct ccagactgga
      301 ttatcttttc attagttatt aatacaaagg agtatcaaaa taaatgaagc cccgtcccga
      361 attcagccac cccgtccatg aagtgatccg gaaacgctgg agcccgcgtt tctttgccga
      421 ccgtccagtc tctgaagagc aggtgctgac tttgtttgaa gcagcccgct gggcggcgtc
      481 cgccatgaac gaacagccct ggcgcttcat ttggtcactc aaagacggtt ccgaaaagta
      541 taaaaagctc ttttcctgcc tatatgaagg caaccagatt tgggcaagct cagcgccttt
      601 gctgatcctc accctcgcca aaacgacctt ttccttcaac ggcaagccca acccctggtc
      661 cagccacgat ttgggcctgg ccgtgggcaa cctcaccacc caggcgtctt tgatggacct
      721 ttttgtgcac aacatgggag gctttgaccg ccaaaaagcc cgcgaaatct tctccttgac
      781 cgaagacctg gaacccatca ccatgatcgc ggtgggttgg gcggaagacc cggaaaacct
      841 ctcagagcag gaacggcaaa gggaatacct gatgcagaaa cgccggtcct tgcaggacat
      901 tttcctgtga caatctgctg tcgttggaag ccggcacact tcttctcatg cggttcttgc
      961 caacgcacta tgtcttagcc atagataaaa cttgacagcc agccctgtgt ttgcttgttt
     1021 agccggaaac aagtcacgaa taaggatgct tgaatgatcc cttccaaaaa gccctgtgtt
     1081 ttgctcctgg ccttgctcct ggccagcctc tgcgccgctc aggaacagcc ggatttttca
     1141 tttcagctcg gccgttacgg caccctgggt tttggttatg gtctgcctta cggcggatac
     1201 ggtttcagcg cggacatgta tatctttgac caattggcgc ttacggtgga cgtaggcact
     1261 ttgctctatg ccgccggcta tgaattgggc attaaatacc tgcacggcaa tgccggcaag
     1321 ctctggcggc cccaagccat cctgctttac ggggtgaacg ggatcatacc ttacaacttt
     1381 tcaaacaccg aggtggtcca aactgaggtt tacaacggtt tcaccgccgg cctgggctcg
     1441 cagttcatgt tcggcaaaaa acgccggcac ggcctcgact tcggcgtcac ctacgttatc
     1501 agctccggcc tgttcaagcg cctggaagag cttgaaggat atgaatttgg agcctccagc
     1561 cgctttggct tctaccttgg ttaccgctac gcgttccagt ttctatattg agcagaggcc
     1621 tcttcagcca ctgttttcct gtttcaggtc gggttgataa acctttcgta atcttgcagt
     1681 tccgacagca agccagccat ataaacatgt aaagaaatgc ttgaaaaaca aacacatccc
     1741 tacacaccat atttcccgaa aacggcagaa gcagtgataa ttggaagcgc cccaccctgg
     1801 cggttttgta ctggcagggc tgagaatctc agggcgggcg acgtggattt cttctatgga
     1861 tccgttgaca accgtttttg gcgcgtgatg cttgaagtgc tgggaacaga gcctggtccg
     1921 ggaacggcgg aactcttaaa gacaatcctg aaaaaccaca aactggggat gggcgatatc
     1981 ctgaaaagct ttacgcgcag agaccgcaag tcatcagatt ccgatctgtc ggagttttcc
     2041 tatgatttca gtctggtgga gggtataatg gaccgggcaa gcatccgcaa gctttttttc
     2101 accagtgagt ttgcttacag gcatttcaaa agggctctcc gagagcaggg ttacaagctt
     2161 aaacagacag ccattgcgag acgccaatac agcctttcgg tcagcaagcc aggttttaaa
     2221 cgggattttg agatcagtgt gctattgtcg ccctcgcgca gggggaggca cagctttgag
     2281 gacctcgttg ccaactataa ggaacagttt tcccaatttt tctaaaacta aactgagatt
     2341 cttgcttagg atgaggtgaa cgcccggcct gttaacttca accctccgca acagctatca
     2401 tagactcagt agctccgcga agacctcagc gcgggtgctt ttgccaacgc tgtgaagggt
     2461 caagcccctt tgccggtgta gtttcagcag cctcaggcag atactggaag ccctgttgcc
     2521 ggcggagaga agttcctgcc actgggctgt gaattctccc ttgttccagt agaggttgag
     2581 gattttggcc agttcctgca gccgggcgac ctgttcaaag ctgagcgcgt cagttttcag
     2641 cacctgccag ggcgggtcgt tcagccacag gaatccacgc tggctggcaa tttcctgcat
     2701 cggcgtgccg ggcagtattt tcagcattcc aagctggatt tctgccggga aggtggaggc
     2761 cagctcgtcg agggaacgca gcacggaagc gtatttttct ccgggcaacc ccaccaacaa
     2821 atcggcgtga acgcagattg ctgtgcgttc gcggagggca ttgagcgcgg ctttcacatt
     2881 gccccagtta gagttgcgcc cacaggctcg ggatacggcg gcgttcacgg tttgcacccc
     2941 aacctcgaag cggatgcggc cgggcggggc tttttccagg attcgcaggt cctcttcggt
     3001 gagcaggtcg ggatagatct caaagtggaa ctcacagctc tgcttctgcc ggatggcgtg
     3061 ttcccagatc aggcgggcca gccggggatg ggcattgaag ctgcggtcaa cgaatttcag
     3121 ggtgcgcggc ttgagttcca gcaagcggtc cagttctgac ctcagcttgc ggcggtcgga
     3181 ggccagggag gggtcgaaac gcgcttcatt gcgtttatcc agagcggaaa ggcaataggc
     3241 gcagcggaag gggcagcccc gtgaactttc ataatagaca agcctgccct gcagagcggg
     3301 acggtctgag cggcggtaga gaaaaggcag ttcgctaagg ggcggagccg gggcctcgta
     3361 agtcccgccg ggaaactcaa atccagattc agccagcctc cggaacactc cctctcccgg
     3421 tccatttacc ctgaaatcgc cttcagccag gccgaaatcg ccgttcgtgg cttcaggtcc
     3481 gcccaatacc actttcaggc ccggcatcag ttttttcagt tccgggataa gccggcgcag
     3541 gtcaaggcta ttccagacgt aagctgaaaa gctggccaca tcagggcggt cgcggacaat
     3601 ttgggtaagg atgtcgaaga gcggttcggc gatggagaat tcacgcaggc aaatctcgaa
     3661 aggcaggccg cgcagggatc cgcgcagata gtaaagcgcg ggattgctgt ggtaccaggc
     3721 gctgttcaac ccgatgaggc tgatcctcag ccgtttattt ttggcggaca ggaacccaaa
     3781 tctccagcac ggaatccggc tgtccccagc ggaaacgcca gtcgtacagt tcaaaatcca
     3841 gatttcccac ccgctcaagc gcgctggcgg gaagccattc tgaatagacc aattcgtagg
     3901 tttggcacaa attgtccagc gccccgcgat gctcaaaaac ggcgtagtca tttgccggaa
     3961 ccacaagcag ttccatgccc gggggaatgt cgttggcgat tgaaacctct acccctgcca
     4021 gataggaaaa cctgtcgccg gagggagcgt cgccctccta gatagcagac gccataggcg
     4081 gtccggtcct tgacggagtg ggggatgccg gcacagacct tgttgaaatc gtcccaaagc
     4141 cgcggaacca cgttgttttg cttgctcgcg cggcacttga agcccaccac cgtgaaggct
     4201 tcgcggctaa ccagtcgggg tttcattcta tctcctttgc ggcgggaccg tatccaatgc
     4261 ggtcccctct tgaggcttca tttttccagc agggcaaggc tgtcaaggga tttgcccggt
     4321 ttttccggca cttacccatt tttccggcca gcctgccttt catttgccgt ggtcctgtcg
     4381 gtcaggccct tagcgctcga gccaatctcc ctcccacgcc cctcgctaaa ttcccgcatc
     4441 aagtgcggcc attgtgcggg aggcggactt gggattcaag aaagggcgtt aacggcccga
     4501 tggcattcca gcttccgcgt ttctgggtga taatctccta cccctcccgg tcccggtcgc
     4561 gttctaagtc tttcctttgc agtgattccc gcttgtcgta ggtctttttc cctttgacga
     4621 gggcgatggt caccttgcag aggccttttt cgttgatgaa gatttccaaa gggacgatgg
     4681 tcattccctg ttcctcggtt ttggcgcgga ggcggcggat ttcgtgtttg tgcagcagca
     4741 acttgcgttt gcgggtgggg tcggggctga agtgggtgga tttttcgaag ggggagatgt
     4801 ggaggttgag cagccagcac tcgccgtcgg tgacgcgggc gtagctgtcc ttgaaattga
     4861 ccttgccggc gcggatgctc tttatttcgc tgcccaccag ggcgatgccg gcttcgaact
     4921 tctggatcac gaagtattcg tgcaaagccc gccggttctt aagcatcata tcagcccaat
     4981 tcctccagca cgcgctgcct ccacatcttg gcgttggcga ttagttcggc ttcgtccact
     5041 ttgcacaggc gcccgccctc cagcacaacc tcaccggcga tcacaacgtc tcgcacctgt
     5101 ttggatccga gggtgtaaac cacgtgggac cagggattgt aaagaggctg gctctcgatt
     5161 ccgccaagct caagaacgca gatgtcggcg tccttgcccg gctccagcga gccgcgtttg
     5221 tccgccacgc cgagggcttt ggcaccgcca atggtggcca tctggacggt ttggacggcc
     5281 ggaaggaagg aggggtcgtt gttcacggcc ttgtgcagtt tggcggtgag gtccatttcg
     5341 gccaggaggt cgaggttgtt gttgctggct acgccgtcgg tggcgaaaca gaggttcacg
     5401 ccgctctcca gatattgggc caggggcagg atgcccgagg cgagcttgag gttggaatca
     5461 ctgcaaatgg ccacggaagc tggattggcg gccagaagag caatttcgtc ggggtcggtc
     5521 cagactccgt gggcgtaaag cgccggaagc tccaaaatgc ccaccttttg caggtaggga
     5581 acgggtttga ggctgtgcgc cttcaggcag tcctccactt cgccgcgggt ctccgccaaa
     5641 tgcgtgtgca acaggattcc gtgtctggca gccgcttcgg cgcattcgcg caaggttttc
     5701 tccgaacagg cgtagatgga gtggggggcg aggttgaaat ccagcaaggg gtcatcggcg
     5761 taacgctggc gcagctccag gaccttgctg ccgagagggg cttcgcgggt gccggaattg
     5821 ccttccagca ccgcttcgcc gatgatggcc ctcagtcctg cttgggagca ggcattcgcc
     5881 actgccggca tatcgaaata catgtcgtgg atttgggtga tgccgttctt gatcatctcc
     5941 gcggcgccgt ggagggcggc ctggctgatg aagtcgcttt ttagcatttt ggcttcgagg
     6001 ggccagatga agtcgtgcag ccaggtctgc aaaggcaaat cgtccgccag acctcggaaa
     6061 tagctcatcg gcagatgggt gtgggcgttg atcagtccag gcatcacgat gcagttggag
     6121 gtgtccagcg tccgccgggc ctgatagctc ttttcccggg ggtcgaagat gtccaggatt
     6181 tggccctggt caatggcgat ggcctggcct tccagaacac gcagcccgct gtccagggtg
     6241 aggatggttc cgccttgcag gagcaggtca atgcttttgt tcataaaggt ttatccctgc
     6301 accttctggg cggcctcggc tgctgcgaaa acgcctttgg gggtgatgat gccctggata
     6361 agttctgccg gggtgatgtc gaaacctgga ttgagaaccg gggagccggg attggcggtg
     6421 atctgtccgc cgtagagctt gatctcctcc tcgtctctga actcgatggg tatctctgtg
     6481 ccctgccggc aggcgaaatc gaaggtggaa agcggcgcgg cgatatagaa gggaataccg
     6541 tggtatttgg ccagcacggc cttgtcataa gtgcctatct tgttggcaat atcgccgttg
     6601 aggcagactc ggtcggcgcc ggtcataacc aggtcgatct cttttttcca gaaataatag
     6661 ccgctggcgc tgtccgggat gatggcatgc ggaatccctt cctgctccag ttcccaggct
     6721 gttatcttcg atccctggtg gcggggacgg gtttcgctca cataaacgaa aatgtctcgc
     6781 cctttgcggt gggccagccg gatcacggcc agtgcggtgc cccaatctac ggtggcaagg
     6841 gcgccggcgt tgcagtgggt gaggattctg gcgccatcgg ggatcaggtg caggccctgt
     6901 tctccgatca gacggcagtc ttcggcgctg cggttggcga actcctttgc ggccagtaaa
     6961 gccactttgc gggcgtgggc caggtcagga atgaatttga tggtgcgttc gtaaacgtag
     7021 ttgaccccgc tttgcagatc aacagcggtg ggacgggtcg cgagaagcag gtcgtgggct
     7081 ttgcggatgt ggctgcggaa cctttcatcc ggagcgttct tcgcggccag ggcgaggccg
     7141 aaagcgcccg cagcgccgat tgccggtgct ccgcgcacgt tcatgttgcg gatggcttca
     7201 gccacctgga ggtggtcgct gaaatccatg atcgtgaaat catgcggaat cctgttctga
     7261 tcgatcatcc gcaggcgccc atgttcccac cagacgctgc ggtattcgcg tccgtcaact
     7321 atcatcggag gtaggctcca caggaatggg gcggttcttg aaacgtgtgt aagcaatgcc
     7381 tccgccgagg gcgagcaaac tggccaatag gccgatgaag ctgaggcgga cgctggtttt
     7441 gtaggattca ggggcgaagg tcagttccag tttgtgcgct cctgcgggaa tctgcaatcc
     7501 gcgcaggatg tagttcgcgg cgtggatggg gatttctttg ccgtccaggg tcgccttcca
     7561 gccggcgggg tagtaaacct cgctcagcac taggaaggcg ggcttgtcgg tatagacgtc
     7621 ataggccagt ttgtgcatct cggccgtggt ttgcgtaacc ctggctgaat caggcttctg
     7681 gatgtctttc aggtccgttt ccacaaaggc cagccggcga ggatcaaagc tctcggaacg
     7741 cagcagcggg aggatggaat cagctggggc gaccttttga acagaatcca cgaaccaggc
     7801 ccggggcagg gcggtttcgt tgcggtaaac cgaagtgccg tcgaagccag tgaaaacagg
     7861 ggtaagcttg ttgtagaact gattgtaggg cggaaagtcc agcgtgaaga gtgaatcgtt
     7921 cggaagcgga tcggggtgaa ggatgtattt ggtggaaagc atgtccatca ccggggtggg
     7981 tttgtcaact ggcatttcgc cttctttcat gccgtacatg cccatcatca ggcggcccca
     8041 ttctccgggc ttctgggtgg cgggatcgcc ttcgatcagc ttcagcagct tgtcatagcg
     8101 ttctagcttg gcggcggaat aaccggtgac agtttggtga tggtaggcaa attcaccggc
     8161 gggtttgccc atcctggctt ctgagagcat gctctggccc aaaggcagga tgcggtagtt
     8221 ggatttgtct gccagaagga attcgtcgta atcctgaggc gcgaaacggt cggtgtattc
     8281 ctgccgggcc tggaggtttt cgctcttgag aaattttccc gtgtaaaccc agaggtcgat
     8341 gaaagccagc agcgtcagca gcagcacgaa cgcaacctga ggcagttttt tcgcgctttt
     8401 aagccaggcc agcgcgaggc tgaggctgag aagcaacagg ctgatcaaac cgctgttgta
     8461 aagtaagccc aggcgggttt ccttaaccgt gcccggaagc tcggtaagtc catgctggga
     8521 taactgttgc aaatccgaag cactggcgaa tccgagaccg ccgaacaggg ctttggctgc
     8581 gatgagccaa aggaagaaaa ccccgccgca gatcagagcc gccttcagca gtcctttgct
     8641 ccagtccttg ctttgctccg cgctgaggct gaggatggtg tccacaccca aagcggcctg
     8701 tagcacggcg ttgatttgca ccatggtgag gatcattgag ggcacgcgga atttattgaa
     8761 gtagggcagg tatttcagga agaggtcaga aagccggggc gtggcgctgc cgaaggacat
     8821 gatcaggaaa atcagcgagc tgatggacag gaaaaccgcc gtgcgacggt gtttggaacc
     8881 gatcagggca atgatcccaa aagccagcac gatcagccca aagtagttgt aaacttgcgt
     8941 aaagggcatg taaccccagt agttggggct cacaccgccg tagaaatccg ggataaagaa
     9001 ggtgatgatt tctttaggat ggaagctcca gccctgggcg taactggttt ccaggccgcc
     9061 gcttccgccg cgctgggtga atttactgta ttccatcgtg ctcaggtagg ggttcatcac
     9121 cgccagcagg gtcagcccca ccgccaccac agccagcaag gtgaagagcc agaagctctt
     9181 atggtctttg gcacggatgc tttccaccag ttgccaaagc cagtagagac ccaggaacag
     9241 atagaggtag tagctgatct gaggatggtt ttcccgtaat tgcatgatca gcgtggtggc
     9301 caggaaaccc aggctcagca ggccgggttt ttgccgcagg cgcatgaagg cccagatcac
     9361 ccaggggatg tacattatag cccggaattt ggtgttgtgg ccgatttcca ccaaacccag
     9421 ccagtggcag gagaacatga aggccacggc cccgaaaaag ctggtccaga catccagttt
     9481 gaggaacctg agcagcaaaa acatccccag cgagccgata aagagcagga agatgcgcca
     9541 gttgatcaac ttatccgtga gccgggaaag gttttccaga aagggccagc ggttcgggaa
     9601 ggagatcatg taggagggca tgccggaaaa catgctttgg gtccagaggg cgttgtcagc
     9661 gtgattttgg ttgtattcga ttatcttgtt cgccgcgcct tgccactgcg agatgtcaga
     9721 tgcatggggg gtcttgccgc cgaaagccac ggggaaatag agcaggctga cgaaaaccag
     9781 cagaaaggcc atcagcgccc aaggcagcca gggtttggcg gaaaggactt ccaaccagcc
     9841 cggggcggcg gccggcgcct gtctcggggt gcgggctgaa gaatgggcgc gggcggcctg
     9901 tttaacgctt ttcacgcctt tatgctgaat ttttgggttg acatttttct tggccatatt
     9961 atcctcaatt caaagatgat tcagtcatca gaattacgcc gcccttatcg gtcaagcaca
    10021 aaatacggcg gcgaatgcca aaagggagac agataatggt tttcgagttt cgcaagcgga
    10081 tttacggctt tgagtgtgac gtttacgggc acctgaacaa cgccaactac ctccaactgc
    10141 tggaaagcgc gaggtctgag gccatgatcg aaatgggtat gtccatgtcc aggatgcatg
    10201 aacttgacat tcaggtcttc atccgcggct tcgagctgga ttaccggcag tccatcgaac
    10261 atgaggacct catcaccatc aaaagctggt ttgatgaaat gaaccgcgtg aaaggccact
    10321 gggtgcagag gatatacaat tcccgcggcg aactttgctt tgaagcgaaa atggtcgccg
    10381 tcttcgccag tggcggaaaa gccacgcggg tgtctgaaga attgttagaa atatttttgg
    10441 gctatctgga gcctccggcg gattgaaaag agtttatggc acaccagatt cgggagccta
    10501 accgacgtta ttcctcgtag ttacctattt gcttattagg ggagggaccc ctgtctgcca
    10561 gatgtcttca caattccctt gacggcgatt cgcggctgag gataatggca tccataaaat
    10621 caaaggaggt aatcgcgatg cgcaagaaga gttgatcctg taacagctct cgcgttgtga
    10681 ttacacccct ttttcacaaa tccccatcga gctgttgctg cccatcccaa aaccagatta
    10741 caggagaaaa ccatgcagtt tatccagctc aaagacatcc actacactta cccggaccag
    10801 tatcagccca tcctcgcggg cgttagcctg gtggtcgccg ctggcgaaaa gatcgccctg
    10861 attggcaaga acggttgcgg caagaccacc cttctgcgga taattttagg cgaattatcc
    10921 cccacccaag gtagtatcag ctatccaagc tccagcccca caatgtctta tcttccccag
    10981 gacatccgcg tctccgcgcc cctcagcgtc cgtgatttcc tcctgcgggt taggccccgg
    11041 caatttgaat tgctcacccg gatcgaatct ctcacttcca cagagaatct ctcttcctca
    11101 gccggactgg aactggcttc cctctggcag gaatacaacg atcagaacac ggcggaatgg
    11161 gaaaaagagg tccatgccat cctcctcggg atggatttgc tgccgctggc ggaacggcct
    11221 tgcgcgaccc tttccggcgg cgaatccacc cgtctccaac tggcggccct gctcctggaa
    11281 aaacctgaca tcctcatcct ggacgaaccc accaaccatc tggattccga acaactgctc
    11341 tggtttgagg actggatgct ggactacggc ggcgctgttc tctacgtatc ccacgaccgg
    11401 gttttcatcg accgcaccgc caccaaaata gccgaactgg aggccggaag gatggaactg
    11461 cgcgcgggca actaccagag cttcgtgcgt gacagacagc agcttaagga ccaccaaatg
    11521 gtgcaatacc gtgagcgtca gcgccttttg cgccagttgc gtgacgccag ccggaaaagg
    11581 cgggcttggg cctcgtcctt tcagaaggaa accagtggcg agggcggtgg ctacgttttt
    11641 gaaatgatct ccaacccagc ccgcacccaa atgcagcagg cccggcacat cgaaaaccgc
    11701 atcaaaatgc tcactgaacg ttatccagta gaaaagccgc atcaggacaa gcttcgccac
    11761 cttgctttcg aagaggtcca ggtgggcgac agggagctga tcagcgtggc tgggatgggc
    11821 tttcgctatc aggaaaactg gatattccgc gatttctacc tccacctcta tggcgcggaa
    11881 aagctctggc ttgccggtcc caacggcagc ggcaagacca ctcttctgca ccttctgctg
    11941 ggttctctgg aaccttgcga gggaacgatt tcccgcgcca accgcctgcg gatcgggtat
    12001 tatgaacagg acctcagcaa gctggatccc caggctgtgg tgctggattt cctgaaaacc
    12061 tctggcaagg aggaaagcca gatacgcact ctcatgggct gcatcggcct gaagacagac
    12121 ctggcctttg cccggacggg cagcctctct tggggcgaac gggccaagtt gcagctactc
    12181 atgttgcttc tgggcgaata caatgtcctg ctgctggatg agcccaccaa ccacctggat
    12241 atccgcagcc gcgaaatgct ggaggaatcc ctggaaaact tcaatggggc cgtcgtcttt
    12301 gtctcccacg accgcgcctt cattaacagg cttgccaccc gaaaggtgga gctagcaaac
    12361 gctccagcct gagatcataa ctcaatggcg cctgaataat aaaagggttc ttgcaaaccg
    12421 ggtggctaaa ttcctccggg ctgatttttg cttgaacaca tgcagccact tgataacaaa
    12481 gagagccaaa tctgcttgcc tgccgctata tcctgtcagc cacaccctta accctcatgg
    12541 ccatcccact cccacgcctc ccgaataata ctcgcatcaa atgcggccat tgtgcgggag
    12601 gcgcgtctgg gcttcaggaa agggccttaa cggccggtcg gcttcgcttc cacgcttcca
    12661 cacgctggac cgagcgagga acaaggatcg acggcaggct ccaaggccac gtcatcccgg
    12721 acgtcaccga gcttgcgagg tgacattgat ccgagatcca tagcggtata gaaactggat
    12781 tccggatcag gcccggaatg acgtaggttt ggggctgagt ggttgtttat gggctatttc
    12841 gcccggttcg gattccgggt caagcctgga aaggcgttgg gaatgggggc aggcagtaaa
    12901 ttgttaaggg caataataag ccggaaagcg gctttgtcca ccctaattga acatcggcca
    12961 caggacaggg ctggaggcgc agattacgtc cggcggcctt gccggtgtaa atttatgttt
    13021 gggcggctga tcagatcagt tttgactctg ggctccgccg gtggtatctc cttccgtgct
    13081 gatgagttcc gtgtccgagg cgggttcgtc ggagtttgtt tcctgggtgg ttgattcctc
    13141 gttggcgtcg gttttatcct gcggaggctg gcaagccgcg agtgtcagca ttccaatgag
    13201 cagggcggcc aacaggatga ggctgatgat cttttgcatg gatcctccta tcagtgattg
    13261 tcacattgat tggttaacaa aaaacgggca tccttgtgga cacccgtttt ttaaggactg
    13321 cgctactaat atcgactagt cgatttagat agggtttaac ttattagtta acgccgttga
    13381 caggggcttg ctgtgccaag gtgtcaacaa tgggggcgac agtagtgtca accacttctt
    13441 cggtggtttc ttcgacaact tcttcggctt cttcggcttt gcaagctgag aaggccagca
    13501 tgccgatgag catcatcgcg agaagagcaa taagagccag ttttttcatt ttgattcctc
    13561 cattcagtga aatccgtatt tttttttggt tttgtttttt tgaattcggt ggcattttat
    13621 agccttaccg gggctttttg ctgagagggc tgaagagccg caaagccttt agcaacctct
    13681 cacacagtcc ttagacactt ctatgtgctt gcttaattgt acccaatcta tataagccgt
    13741 attttttggc aagttatttt ttcaaaagtt cgattccaga ccggcgtgaa ccattcccgc
    13801 cgagaagtcc gcgaagccgt tttcccggta tccgagggca tactcgatgc tcagaattcc
    13861 cagcctggtg cgcagtttga tgcctagtcc gggggcgagg aggtcggcgc gcaggctgtt
    13921 cttgtcccgc gcgaggaggc cgtggtcgta aaaaaggtag atccgggcct gggaactgat
    13981 cagatagcgg agttcgagcg aagcccagcc gaggcgccaa ctgctgaatt cgtcttcgcg
    14041 gtagccccgc agcgagttgt agccgcccag ccggtaagtt tcataagcca cggaactgct
    14101 gtcggcgagg ctgcgcagat gaacgcccag ggcgcaggtc cagcgcggcg ccaggccaat
    14161 gtattgggtg tggtcggcct ccagggcgtt gttccagccg cttcgcttgc tttggtgcag
    14221 gcggtaggtg atgttggtct gcattccctt ggaagggttg aaagcgcggt cccggctgtc
    14281 gagtcgccaa aaggcgccga tgccgcggct ggaggtggtt tccaccagca ggctgtcctt
    14341 tgcggcgggc acatagtagt taacgctttc cacggccagt tccaggccgt agcggtgaac
    14401 gtcgtaatag gaatagatat cggcgcccac ggccgatttg atgaagctgt ctttttcctc
    14461 ctcgcgggaa agcctgaggt cgccggaaag gggaaagacg ttcggcccgg attcgtggta
    14521 tgaaaagcgg agcaggctgg tgagcggcag ctttctccag ttcaaggtca gggagcggtc
    14581 gctgccccag aggttcagga aaccgaggtt gaggaagccg gtgaattcgt ttttcgcgcc
    14641 tacccgggtg aagcccaaaa cgccttccag agaggtcatt ttgccttcct cgatcttgat
    14701 cagcaggctg gcggggtcca ccggttcgat caagcaggcc tcgatgtagc ttttgcccaa
    14761 aatcctgtcc tcggcggcgc ggatggcctc aggggtaacc acctgcccgg agctgaagcc
    14821 ggccagtctg atgagggtct ggtcgcgggt gtatttgttg ccctggaagt agtatttttc
    14881 aggcttgaga ggcttgcctt cttcgatgcc gatgtaggcg gcgaggtttt cgcccagggt
    14941 cagggaatcc agccccacac tggcaaagag gtagccgcgg cggtgatact catccagaat
    15001 ccggtccatg gtgcgcggca gttcggaaag cccgaccttg cttttatcaa caagcaggag
    15061 cagttcacgc agctttgctt cagagaaata gcgcaggccg cggaagcgga gttcgcaatc
    15121 ctgtgccggc aatacctccg tgagcttgaa agccagttcc aggctgcttt cagagagggg
    15181 caccagttcc ggattggcga ttttcacgaa aggatgtccg tttgcctgta gccaagcctg
    15241 catcagggcg atggcggcgt tgacgtccgc gggggtgtat tcgcttccgg cagtcagtcc
    15301 ggaagccttc agcaatgcgg tctcgtcgaa gggaaaattg gcagagaaac tgaccttgtc
    15361 cacgctcagg gcaggggcca aaacccaggc cagaagaaga gctgcggcca atagggcgcg
    15421 tttcatggcc ggaaaatcaa tcgcggtccg ccttcagcac ggccagaaag gcttcctgcg
    15481 gcacggtcac ggagccgatc tccttcatct tcttcttgcc ttccttctgt ttttccagca
    15541 gcttgcgttt gcggctgacg tcgccgccgt agcattttgc cagcacatcc ttgcgcatgg
    15601 c
//
LOCUS       DAOOWR010000005        15296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_3895_length_15296_cov_3.606325, whole genome shotgun sequence.
ACCESSION   DAOOWR010000005 DAOOWR010000000
VERSION     DAOOWR010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_15296_cov_3.606325"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="secY"
                     /locus_tag="PLB85_00310"
     CDS             complement(<1..1124)
                     /gene="secY"
                     /locus_tag="PLB85_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU71798.1"
                     /translation="MFKTIANIFRIPDLRKKILITALLLVLYRLGSFIPIPGVDAVQL
                     SSFFQRQEGGLFDLLNLFVGGNFERASIFALGIMPYITASIVIQLLGSIIPYFEKLRK
                     EGADGQKKMNQITRYFTVALAAFNAITITVGLVNMTGAGGPVVPQPNFLFHLTGVITL
                     ITGTMIVMWLGEQITEYGIGNGISLIIFAGIIARYPEGFITLFKTRFHSLSGVLISLA
                     AIVVMVAVTAAIIFVTEAVRKIPVQYAKRIVGRKVYGGQSTYIPLRVNTAGVIPIIFA
                     QSILMFPATLITLFQGGEAEAGTFWYNLRTMFTPGHWLYTIIYVGLIIFFAYFYTALV
                     LNPTEMAENMVKYGGHIPGKKPGKKTAEYISSVLTRITLPG"
     gene            complement(1126..1581)
                     /gene="rplO"
                     /locus_tag="PLB85_00315"
     CDS             complement(1126..1581)
                     /gene="rplO"
                     /locus_tag="PLB85_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU71799.1"
                     /translation="MLTLSNLGKPAGRKARKRLGKGEGSGLGQQSGRGHKGIKARSGG
                     KVPAYFEGGQMPINRRLPKRGFKNPYRKNYRVLNLSRLVGLEETEFDISRLELLGLIP
                     VKGKKGSYPVKVLASVSEEFTKTVHIKANAFSQRAIQLIEANGGKAEVV"
     gene            complement(1583..1768)
                     /gene="rpmD"
                     /locus_tag="PLB85_00320"
     CDS             complement(1583..1768)
                     /gene="rpmD"
                     /locus_tag="PLB85_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU71800.1"
                     /translation="MKIRVTQIRSTINRKENHKRIIKSLGLGNPGKTRIHDDNPCIRG
                     MINKVSYLLKVEEVKGE"
     gene            complement(1765..2265)
                     /gene="rpsE"
                     /locus_tag="PLB85_00325"
     CDS             complement(1765..2265)
                     /gene="rpsE"
                     /locus_tag="PLB85_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU71801.1"
                     /translation="MPDEEKLFETVIDTKRVAKVVKGGRNFSFSAIVVVGDRAGKIGV
                     GDGKANEIADAIRKAKEKATKSMFTVPIIKGTIPHEIMSRFGASRVMMKPASPGTGVI
                     AGGTTRAIFEAAGIQNILCKSLGSNTPCNVVKATVNGLKSMRTLNDIARLRNKSVAEL
                     TGQEEK"
     gene            complement(2294..2665)
                     /gene="rplR"
                     /locus_tag="PLB85_00330"
     CDS             complement(2294..2665)
                     /gene="rplR"
                     /locus_tag="PLB85_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU71802.1"
                     /translation="MITPSNIAKHALRNRRRAAIRKKLRGTTERPRLVVFRSLKHIYA
                     QIIDDSTGRTLTSASSRSKDIELEKGIKKTEQGFEVGKKLGEKAIAAGISKIAFDRAG
                     YKYHGRVKALAEGARKAGLDF"
     gene            complement(2681..3253)
                     /gene="rplF"
                     /locus_tag="PLB85_00335"
     CDS             complement(2681..3253)
                     /gene="rplF"
                     /locus_tag="PLB85_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU71803.1"
                     /translation="MSRIGKAPVKFDANTKVEVKDNVIYVKGKLGELHYQLMPGMSVE
                     LGDGVINVKRNDNSKTQRAVHGLTRALIQNMVIGVADGYMKTLNVIGTGYSAERQGPW
                     LILSLGYSHDIVLEVPEGLTVEAEAVPRSKTVRSDLQSIIRIKGIDKQLVGHFAAEVR
                     SCRPPENYKGKGVRYENEQVTIKAGKAGTK"
     gene            complement(3266..3679)
                     /gene="rpsH"
                     /locus_tag="PLB85_00340"
     CDS             complement(3266..3679)
                     /gene="rpsH"
                     /locus_tag="PLB85_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU71804.1"
                     /translation="MSVSDPIADALTKIRNAYRAGHPQVIVNHSRLVEALTKILAAEN
                     FVNSVQVLDKDPEQKINYRRILVNLRYTNDGKPIIQGLVRISKPGRRVYVKADKIPSV
                     YNNTGCAVISTSQGVMVDRDARLNRVGGEYICKVW"
     gene            complement(3696..3881)
                     /locus_tag="PLB85_00345"
     CDS             complement(3696..3881)
                     /locus_tag="PLB85_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOU71805.1"
                     /translation="MAKKSLILKQQRTPKFKVRKYNRCKICGRPRAYMRDFGMCRLCF
                     RKYASQGQIPGITRSSW"
     gene            complement(3891..4436)
                     /gene="rplE"
                     /locus_tag="PLB85_00350"
     CDS             complement(3891..4436)
                     /gene="rplE"
                     /locus_tag="PLB85_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU71806.1"
                     /translation="MNRIKEQYRKTVVPALQKRFAYKNPHQVPRLDKIVVSMGVGDAT
                     QNKALLDNAVKDLEQITGRKVIITKARKSISNFKLRQGMPIGCKVTLRDEIMYEFFDR
                     LVAIVIPRMRDFRGIPTDSFDGRGNFSLGIKEQTVFPEIEYDKIDAVRGMNINIVTTA
                     HTDDEARELLRELGMPFQRSE"
     gene            complement(4448..4765)
                     /gene="rplX"
                     /locus_tag="PLB85_00355"
     CDS             complement(4448..4765)
                     /gene="rplX"
                     /locus_tag="PLB85_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU71807.1"
                     /translation="MPDKLKLKKGDYVIVISGNYKGRKGRVLKAYPKTNRVIVEKVHM
                     IKKHTKPSQNNPQGGIISKEAPINASNVMLFNEKLNTVSKPVIQIREGHRVRVCKKSG
                     DEL"
     gene            complement(4775..5143)
                     /gene="rplN"
                     /locus_tag="PLB85_00360"
     CDS             complement(4775..5143)
                     /gene="rplN"
                     /locus_tag="PLB85_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU71808.1"
                     /translation="MIQAQTMLNIADNSGAKKAMCIKVLGGTRRKYASVGDVIVVAIK
                     SATPGGKVKKGSVERAVIVRTAKEIGRSDGSYIRFADNAAVIIDEKLDPKGTRIFGPV
                     ARELREQAYMKIVSLAPEVL"
     gene            complement(5159..5419)
                     /gene="rpsQ"
                     /locus_tag="PLB85_00365"
     CDS             complement(5159..5419)
                     /gene="rpsQ"
                     /locus_tag="PLB85_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU71809.1"
                     /translation="MTVADTRKVIKQGVVVSDKNDKTIVVRVLRQYIHPLYKKTVRRH
                     KKFMAHDENNEAHEGDIVQIVEHRPMSARKRWALHKIVERSK"
     gene            complement(5416..5607)
                     /gene="rpmC"
                     /locus_tag="PLB85_00370"
     CDS             complement(5416..5607)
                     /gene="rpmC"
                     /locus_tag="PLB85_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOU71810.1"
                     /translation="MKIDDIREMSVTDLHEKLEELRVELFNLRFQKSKNLLDRTDRLR
                     IVRRNIARINTVLNAKEER"
     gene            complement(5604..6023)
                     /gene="rplP"
                     /locus_tag="PLB85_00375"
     CDS             complement(5604..6023)
                     /gene="rplP"
                     /locus_tag="PLB85_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU71811.1"
                     /translation="MLAPKKVRHRKMMKGRRNGLAWTGSNIDFGDYGLIALEDAFISS
                     RQIEAARIAITRHMKRVGKVWIRIFPDKPITSKPAETRMGKGKGAPEYWVAVVRPGRV
                     LFELEGVDVKVAKEAMRLAAHKLPIRTRVIAREGVEL"
     gene            complement(6034..6681)
                     /gene="rpsC"
                     /locus_tag="PLB85_00380"
     CDS             complement(6034..6681)
                     /gene="rpsC"
                     /locus_tag="PLB85_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU71812.1"
                     /translation="MGQKIHPILYRLGVNKDAESIWFAQGSTYVDFLQEDIQIRKYIH
                     KRLDQKMVSRVQISRKTSSITIDIHTARPGLVIGKKGEDIDRLRNELNVLINKDRKNP
                     ITVSINIEQIDKMWLDARLVGLEIARQLEERVAFRRAMKLAIRNVMKEGAQGVKVQVS
                     GRLGGAEIARTERYKQGRTPLHTLRADIDYATVEANTTYGVIGIKVWIYKGDILG"
     gene            complement(6692..7033)
                     /gene="rplV"
                     /locus_tag="PLB85_00385"
     CDS             complement(6692..7033)
                     /gene="rplV"
                     /locus_tag="PLB85_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU71813.1"
                     /translation="MEATAKLRFARGSARKARLVLDQIRYKSVPEAQGILQFSRRAAA
                     KPIGKLLASAVANAQVKDPRLDLSRVYVTEAMADSGPQMKRYMPRAHGRAYMIRKQTC
                     HIALEIRTIEE"
     gene            complement(7046..7318)
                     /gene="rpsS"
                     /locus_tag="PLB85_00390"
     CDS             complement(7046..7318)
                     /gene="rpsS"
                     /locus_tag="PLB85_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU71814.1"
                     /translation="MARSIKKGPFVDDHLLKKVETLNANNKKNVIKTWSRRSMIIPSF
                     IGHTFSVHNGHKFVPVYVTENMVGHKLGEFSPTRTYRGHKDKKKGK"
     gene            complement(7331..8161)
                     /gene="rplB"
                     /locus_tag="PLB85_00395"
     CDS             complement(7331..8161)
                     /gene="rplB"
                     /locus_tag="PLB85_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU71815.1"
                     /translation="MGIKKYKPTTPSMRFRTGYTFTEITTDSPEKSLLKPMRKTGGRN
                     NKGRITCRHRGGGHRRHYRVIDFKRDKFGIPAKVASIEYDPNRTARIALLHYVDGEKR
                     YIIAPEGLTVGSKVMSGPEAEIALGNALPLERIPLGSTVHNIELKKGRGGQIARSAGA
                     YGQVVAKDGDYVHVKMPSNDVHLIRKECLATMGQVSNQDHNLLQLGKAGRKRWMGIRP
                     TVRGVVMNPVDHPMGGGEGKSSGGRHPVSPWGKSSKGGKTRKTRKYSDKYIVKAVKKR
                     "
     gene            complement(8172..8459)
                     /gene="rplW"
                     /locus_tag="PLB85_00400"
     CDS             complement(8172..8459)
                     /gene="rplW"
                     /locus_tag="PLB85_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU71816.1"
                     /translation="MIHPRNIIISPIITEKSSNQIERTNTYSFKVSINANKIEIKHAI
                     EKIFSVKVLDVNTIRMLGKPKRLGRYSGKRPDWKKAIVTLRDGDKIAEFEA"
     gene            complement(8456..9091)
                     /gene="rplD"
                     /locus_tag="PLB85_00405"
     CDS             complement(8456..9091)
                     /gene="rplD"
                     /locus_tag="PLB85_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU71817.1"
                     /translation="MVKAIKFNSLGERMEEIELPANIFDVDVNSPKVLLHEVITMYLG
                     NQRQGTVQKKNRAMTAGSTRKLFKQKGTGNARQGTRRTPVRVHGGRAFAILPKDWYRT
                     IPRTKKRMALKVALTDRAREGRVFIVDKLEFDTPNTKKALELLGKMIPERGRKLVVTD
                     GHHQPTVKSFANLPDVITDAADSLYAYEILNSSYILMTQEALNKVEEVFSK"
     gene            complement(9102..9734)
                     /gene="rplC"
                     /locus_tag="PLB85_00410"
     CDS             complement(9102..9734)
                     /gene="rplC"
                     /locus_tag="PLB85_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU71818.1"
                     /translation="MLGLIGKKIGMTQIFDDNGKLIPVTVIQAGPCRVVCKRSKDQHG
                     YEALQMGYEELPEKRVKKPLKGYFKKNDSPFFRYLREFRPAFGQQVDDYEVGSELKVD
                     WFTTAETVCVRANSKGRGYTGVMKRHGFAGFMASHGVHESFRGGGSIGQCAQPSRVFK
                     GKKMAGQHGNRQVSVRHLQVVKVDAEQNLIMVKGAVPGHRNSLVTIHKEH"
     gene            complement(9737..10063)
                     /gene="rpsJ"
                     /locus_tag="PLB85_00415"
     CDS             complement(9737..10063)
                     /gene="rpsJ"
                     /locus_tag="PLB85_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU71819.1"
                     /translation="MSKSENRIRIKLKAYDHRLLDHSMSEIVKSTRNTGAKVIGPIPL
                     PTDKTLYTILRSPHADKKSQDQFQMLVHKRLIDIMNPTQQTTNALKKLSLPAGVHVEI
                     KANTRS"
     gene            10821..12917
                     /locus_tag="PLB85_00420"
     CDS             10821..12917
                     /locus_tag="PLB85_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71820.1"
                     /translation="MKVRELTIAIILICFFSCRAYALSIQEQISQSTKLDFQNGIMGN
                     VDDNFSPCRLNTEFEFYADGRNLYMICAVEIDSTFNLGNETKRDQSGSGDFIMLRLIT
                     VPNQNFAYTYVFYPLMNKEDYTLGLGLTQNYEWNSDYEYESVINQDKWTIYARIPWKD
                     MRTTKDTPFIVGVIANRWMRNEGRGFNYPHVVRSMGEAYYQGAAPLTVPVRIARDYGL
                     NARLYYTAKYDLLNNQNYKVKDYIGLDFAFRPQQNGSLKLTLQPDYSDIPLDSEDDIY
                     NTLYKPWLQENRFFFKEDLDLLTGSNSYWYSRNINSPIAAIKYSLIQPKTSLIVLSAK
                     DQKKQLYNGTDDFYNALAVRQKIGKSQLSLYAYNRMDKKAAYNNEVASIMVSRQFWGN
                     HLLSLELGGSVTRDSLPADYSSGSSGILKYGFSNIKHTLDLSGKYIDDSFRADMGNIS
                     LKNRNTFNATYTNYLNFPRSFISDFSNSLTAEYVSDHNTNDRIEAYLAFRNQSKILST
                     RAYFGIDASYGDETYNDIRYEYKKAFLSLGSNYLSNISPFIRLGGARSLVYRLEKLVN
                     WAYLYGSLESLINPNTAVKLDATYYKYDHPKTENWDNEYLFANLDYAINFNTGFRFNM
                     GLRFNNIEIPAAMYDGYFGYYFNSEWQVNRILRVNLGFQSKADHYSYSIDPNPTIYHL
                     YDITSQNIYLKTSISF"
     gene            complement(14103..>15296)
                     /locus_tag="PLB85_00425"
     CDS             complement(14103..>15296)
                     /locus_tag="PLB85_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU71821.1"
                     /translation="NFENGGSANLGNFGPGETKTFSVDITVGSEAWHGRNLPMKMVVS
                     SNGYSNTAFYSLTAGTPATTSPTGPDEYGYFAYDSFDAGFVQTPVYNWIETDPALGGQ
                     GTVWEVMDDGSLTVDLPFSFRFYGREYNHVTMCSNGWISLLPTDMVDFYNCYIPAALG
                     PYAMIAGYWDDLKGMKTGDNTFSNMRLIRWHDAANNRYIVQWNDAYNQYNIDLMENAS
                     LEKFQIILYPQADRDGDIVVQYHTVDNPGLTTNYCTVGIENHTQTVGLGYTHGNVYPP
                     TATPLQAGLAIKFTTLAPDSYVAANDPVAVPFALEQNYPNPFNPSTTLSFSLAQSGQA
                     RLAVYNTKGQLIRTLVDSELPGGKHSVVWNGLDEAGKSVSSGVYLYKLETAGASQTRR
                     MLLMK"
BASE COUNT         3121 a         3951 c         4219 g         4005 t
ORIGIN      
        1 ccgggcaacg tgatgcgggt aagcacagag gagatgtatt ccgcggtctt tttgccgggt
       61 ttcttgcccg ggatgtgtcc accgtatttg accatatttt ctgccatttc agtgggattg
      121 agcaccaagg cggtgtagaa ataggcgaag aagatgatca ggccaacgta gatgatggta
      181 tacaaccagt gaccgggcgt gaacatcgtg cgcagattgt accagaaggt gccagcctca
      241 gcctcgccgc cctggaagag ggtgatgagc gtggccggga acatgaggat cgactgcgcg
      301 aagatgatgg ggataacgcc ggcggtgttc accctaagcg gaatataagt gctctgccca
      361 ccatagacct tgcggcccac gatgcgtttg gcatactgga ccggaatctt gcggacggct
      421 tcagtcacga agatgatcgc cgctgtaaca gcaaccatca caacgatggc ggccagggag
      481 atgagcactc cggacaagct gtggaaccgg gttttgaaga gggtgatgaa accttcgggg
      541 taacgggcga tgatcccagc gaagattatc agcgaaatgc cgtttccaat tccgtattcg
      601 gtgatttgct ctcccagcca catcactatc atagtgccgg tgatcaacgt gatcactccg
      661 gttaggtgga agaggaagtt cggctgagga accactgggc cgccggctcc ggtcatgttg
      721 accaggccta cggtgatcgt gatagcgttg aaagcggcca gtgcgaccgt gaaataacgg
      781 gtgatctggt tcatcttctt ctggccgtca gcgccttcct tgcgcagctt ttcaaaatag
      841 gggatgatgc tgcccaagag ttggatcacg atcgaagctg taatataagg catgataccc
      901 agggcaaaga tcgaggcgcg ctcgaagttt ccgcccacga agaggtttag caggtcaaag
      961 agaccgcctt cctgtcgctg gaagaaagag ctcagctgca ccgcatccac tccgggaatc
     1021 ggaatgaagc ttcccaggcg atacagtacc agcagcaggg ccgtgatcag gatctttttg
     1081 cgcaggtccg ggatacggaa gatgttcgcg atggtcttga acaacttaca ccacctctgc
     1141 cttgccaccg ttcgcttcga tcagctggat ggcgcgctgg gaaaaagcgt tggctttgat
     1201 gtgaacggtc tttgtgaatt cttcgcttac actggccaga actttcacgg ggtaggaacc
     1261 ctttttgcct ttcacgggta tcaggcccaa caattccagc cggctgatat caaattccgt
     1321 ctcttccagt cccacaaggc gggaaaggtt gagcacgcgg tagttcttgc ggtaggggtt
     1381 tttgaagccc cgcttgggca gacgccggtt tataggcatc tggccgcctt cgaaataggc
     1441 gggaaccttg ccaccggacc gggcttttat acctttgtgg ccgcggccgg attgctgtcc
     1501 cagaccggat ccctcgcctt tgcccaagcg ttttctagct tttctgccag cgggtttgcc
     1561 aagattggaa agggttagca tgttattctc cctttacttc ttctactttg agcaggtacg
     1621 atactttgtt gatcataccg cggatgcagg gattgtcatc atgtatcctg gtttttccgg
     1681 gattgcccag ccccagggac ttgatgatcc gtttatggtt ttcttttctg tttattgtgc
     1741 tgcggatttg ggtcaccctg atcttcattt ttcctcctga cctgtgagtt cagccacaga
     1801 tttgttgcgc aggcgggcaa tgtcgttcag ggtgcgcatg gacttgagcc cgttgacagt
     1861 ggctttcacc acgttgcagg gggtgttcga gcccagggat ttgcagagga tgttctggat
     1921 cccggccgct tcaaagatgg cgcgggttgt cccgccggca atcacgccgg tacccgggga
     1981 agcgggcttc atcatcacgc ggctggcgcc gaaacgagac atgatctcgt gcgggatggt
     2041 gcctttgatg atgggcacgg tgaacatcga tttggtggct ttttccttgg ctttgcggat
     2101 ggcatcggca atttcattgg ctttaccgtc tcccacgccg attttgccgg cacggtcgcc
     2161 aaccacaact atggcgctga aactgaagtt acgtccgcct ttcactactt tggccacacg
     2221 cttggtatca atcactgtct caaacagttt ttcttcatcg ggcaggttgt gttcgtaatt
     2281 caagtttcct ccttcagaaa tcaaggccag ccttgcgggc gccttcagcc agggctttca
     2341 cccggccgtg gtatttgtag ccggcgcggt cgaaggcgat tttcgagatg ccggcggcga
     2401 ttgctttttc acccagtttt tttcccactt cgaagccctg ttcggtcttc ttgatgcctt
     2461 tttcaagctc gatgtccttg ctacgcgagg aagcggatgt caaagtacga cctgtgctgt
     2521 cgtcaatgat ctgggcgtag atgtgtttta ggctgcggaa aacgaccaga cggggccgct
     2581 cggttgtgcc acgcagtttt ttgcgtatgg cagcacggcg gcggttgcga agggcgtgtt
     2641 tggctatgtt gcttggtgtt atcatgatcg gctcctcgct ttacttggtc ccggcttttc
     2701 cggctttgat ggttacttgc tcgttctcat accgcacacc tttgcccttg tagttttcgg
     2761 gcggccggca ggaacgcact tcggcggcga aatggccgac gagttgtttg tcgatgccct
     2821 tgatgcggat gatgctctgc aggtcggagc gaacggtttt cgaacggggc actgcctcgg
     2881 cttccactgt aagtccttcc ggcacttcca gcacgatgtc gtgggaatag cccaggctta
     2941 gtatcaacca gggtccctgc cgctcggcgg aatatccggt cccgatcacg ttgagggtct
     3001 tcatgtagcc gtcagccacg ccgatgacca tgttctggat cagagcgcgg gtgagtccgt
     3061 gaacggcgcg ctgggtcttg ctgttgtcat tgcgtttcac gttgatgacg ccgtccccga
     3121 gctcaacgct catgcccggc atcagttggt aatgcagttc gcccagtttt cccttcacgt
     3181 aaatcacgtt gtctttcact tccactttgg tgttggcgtc aaatttgacg ggggcttttc
     3241 ctatgcgtga catttaaaaa acctcctacc aaaccttgca gatgtattcg ccgccaacac
     3301 ggttcagcct ggcgtcgcgg tcaaccatca ccccctggga ggtggagatc accgcgcagc
     3361 ctgtgttgtt gtacacactt ggaatcttgt cggctttcac atacacgcgc cttcccggct
     3421 tggagatcct caccagtcct tgaatgatgg gcttgccatc attggtatag cgaagattca
     3481 ccaggatgcg gcggtaattt attttctgtt ccggatcttt gtcgagaacc tggacgctgt
     3541 tcacgaaatt ctccgcggcg aggatcttgg tcagcgcctc cacgaggcgg ctgtggttca
     3601 cgatcacctg cggatgtccg gcacgatatg cattgcgtat tttggtcaat gcatcagcta
     3661 tcggatcgct tacgctcatt cgtatgctcc tttgttcacc agctggatcg ggtgatcccg
     3721 gggatttggc cctgggacgc gtatttgcgg aaacacaggc ggcacatgcc gaaatcgcgc
     3781 atgtaggccc tagggcgtcc gcagatcttg cagcggttgt attttctaac tttgaatttg
     3841 ggtgttcttt gttgtttcag gatcagtgat ttcttggcca tcgattctcc ttattcactg
     3901 cgctggaagg gcattccaag ctcccgcagc agttcgcggg cttcgtcatc ggtatgcgcg
     3961 gtggttacaa tgttgatgtt catgccgcgc acagcgtcga tcttgtcata ttcgatctcg
     4021 gggaacacgg tctgttcctt gatgccgagg gagaaattcc ctctgccatc gaacgaatcg
     4081 gtcgggattc cgcggaaatc gcgcatgcgg gggatcacta tggccaccag ccgatcgaag
     4141 aactcataca tgatctcgtc acgcagagtg actttgcagc cgatgggcat gccctggcgc
     4201 aatttgaagt tggaaatgga cttgcgggcc ttggtgatga tcaccttgcg tccggtgatc
     4261 tgttccaggt ccttcactgc gttatccagc aaggctttgt tctgggtggc gtcgccaact
     4321 cccatgctca ccacgatctt gtccagacgc ggcacctgat ggggattctt gtaggcaaag
     4381 cgtttttgca gagccggaac cactgtttta cgatactgtt ctttgattct gttcatgtgg
     4441 ttctctccta cagctcgtca ccggattttt tgcacacgcg gacacggtgt ccttcccgga
     4501 tttgaatcac cggtttggaa accgtgttca gcttttcgtt gaagagcatc acattggaag
     4561 cgttgatggg cgcctccttg gaaatgatgc cgccctgggg gttgttctgg gagggcttgg
     4621 tgtgtttttt gatcatgtgc actttttcca cgatgacccg gttggtcttg ggataagctt
     4681 tcagcacgcg gcctttgcgg cctttgtagt tgccgctgat cacgatcacg tagtcacctt
     4741 tcttgagttt gagtttgtct ggcatcatat actcctacaa cacttccggg gccagggaga
     4801 ctatcttcat gtaggcctgc tcgcgcagtt cgcgggccac cgggccgaag atgcgggttc
     4861 ccttgggatc gagcttttca tcgataataa cggcggcgtt gtcggcaaaa cggatgtatg
     4921 atccgtccga acgtccgatc tctttcgcgg tgcgaactat cacggcacgt tccacgctgc
     4981 cttttttaac tttgccaccc ggggtggcag atttgatcgc aacaactatg acgtcgccaa
     5041 cgctggcgta tttacgtctg gtgccgccaa gcactttgat gcacatggct tttttggcac
     5101 cggagttatc ggcgatattc aacattgttt gggcttgaat cattccaaaa ctcccatatt
     5161 atttgctgcg ctctacgatc ttgtgcaggg cccagcgttt gcgtgcgctc atggggcggt
     5221 gttccacgat ttgcactatg tcgccttcat gagcttcgtt gttctcgtcg tgggccatga
     5281 actttttatg ccggcgcacg gtctttttat acagcgggtg gatgtactgg cggagaactc
     5341 ggacgacgat ggttttatcg ttcttgtcgc tcaccaccac accctgtttg atcactttac
     5401 gggtatcagc cacggtcacc tctcttcctt tgcattgagt actgtattga tgcgggcaat
     5461 gtttcttctc acgatgcgca ggcggtctgt ccggtccagc aggttcttgg atttctggaa
     5521 gcggaggttg aaaagctcca cacgcagttc ctcgagcttt tcgtgaaggt ctgtcaccga
     5581 catttcgcgg atatcatcga tcttcataat tcaactcctt cgcgggcgat aactcgggtc
     5641 ctgatcggaa gcttgtgtgc ggcaaggcgc atggcttcct tggcaacctt gacgtccact
     5701 ccttcgagtt caaagaggac gcggccgggg cgaaccacag ccacccagta ttcgggagcg
     5761 cccttgcctt tgcccatacg ggtttcggcc ggtttactgg tgatcggctt gtccggaaat
     5821 atccggatcc atactttgcc aacgcgcttc atgtgcctgg ttatggcgat acgcgcggcc
     5881 tctatttggc ggctgctgat gaaggcgtct tccagggcga tcaaaccgta atcgccgaag
     5941 tcgatgttgc tgccggtcca ggccaggccg ttgcggcggc ccttcatcat cttgcggtgt
     6001 cttacttttt ttggtgctaa catttacctt ctcctatccc aggatgtcgc ccttgtagat
     6061 ccacaccttg atgccgatca cgccgtaggt ggtgtttgct tccacggtgg catagtcgat
     6121 gtcggcacgc agggtgtgca gcggggtgcg gccctgtttg tagcgctcgg tgcgcgcgat
     6181 ctccgctccg ccaaggcgtc cggaaacctg aactttcacg ccctgggctc cttccttcat
     6241 cacattgcgg atggccagct tcatggcccg gcggaaagcg acgcgctctt ccagctgacg
     6301 ggcgatctcg aggcccacca aacgcgcgtc cagccacatc ttgtcgattt gctcgatatt
     6361 gatggagact gtgatcgggt ttttgcggtc cttgttgatc agaacgttca gctcgttgcg
     6421 caggcggtcg atgtcctcgc cttttttgcc gatcaccagg ccgggacggg ctgtgtggat
     6481 atcgatggtg atcgaactgg ttttgcggga tatctgcacc ctggagacca tcttctggtc
     6541 cagtcgtttg tggatgtatt tgcggatctg gatgtcttcc tggagaaaat ccacataggt
     6601 ggaaccctgg gcaaaccaga tcgattcggc gtctttgtta acgccgaggc ggtacagaat
     6661 ggggtgtatc ttttgtccca aggtaatcct cctattcctc tattgtccgt atttccagag
     6721 cgatatggca ggtttgcttg cggatcatgt aggcccgtcc atgtgctctg ggcatataac
     6781 gtttcatttg ggggccggaa tcggccatgg cttcggtcac atagacccgg ctgaggtcga
     6841 gcctggggtc tttcacctgg gcgttggcca ccgctgaggc cagcagcttg ccgatcggct
     6901 tggccgcggc ccggcgcgag aattgcagga tgccctgcgc ttcgggtacg ctcttgtagc
     6961 ggatctggtc caaaaccaga cgtgctttgc gggcggaacc gcgggcaaaa cggagtttgg
     7021 ctgttgcttc cattatctat gctccttatt tacccttttt cttgtctttg tggccacggt
     7081 aggtgcgcgt cggcgaaaac tcgcccagct tgtgccccac catgttttcg gtcacataca
     7141 ccggcacaaa cttatgtccg ttgtgcaccg aaaaggtatg tccgataaaa gagggtatga
     7201 tcatggaacg gcgggaccag gtcttgatca catttttctt gttgttggcg ttcagcgttt
     7261 ccactttctt cagcagatga tcgtcaacaa agggaccttt cttgattgaa cgtgccattg
     7321 tttccttcct ttatctcttc ttcacggctt tcacgatata tttatcggaa tacttgcggg
     7381 ttttacgggt tttgccgccc ttggaggatt tgccccaggg tgacacggga tggcgtccgc
     7441 cggaggattt tccttcaccg ccgcccatcg ggtggtccac agggttcatc accacgccgc
     7501 gcacggtcgg gcgaatgccc atccagcgtt tgcggccggc tttgccaagc tgcagcagat
     7561 tgtggtcctg attgctcacc tggcccatgg tggccaggca ttccttgcgg atgaggtgga
     7621 catcgttcga gggcattttc acatgcacat agtcgccgtc tttggccaca acctggccgt
     7681 aggcgccggc ggaacgggcg atctgaccgc cgcggccttt cttcagttcg atgttgtgca
     7741 cggtggagcc cagcgggatg cgttccaggg gcagcgcgtt gcccaaagct atctcagctt
     7801 cggggccgga catcactttc gatcccacgg tcaggccttc gggagcgatg atgtagcgtt
     7861 tttcgccgtc cacatagtgc agcagggcga tgcgcgcggt gcggtttgga tcgtattcaa
     7921 tgctggcgac tttggccggg atgccgaatt tgtcgcgctt gaagtcgatc acccggtagt
     7981 ggcggcgatg cccgcctccg cggtggcggc aggtgatcct gcccttgttg ttgcggccgc
     8041 cggtcttgcg cattggcttg agcaacgatt tttccggaga gtcagtggtg atctctgtaa
     8101 atgtgtagcc cgtgcggaac cgcatgctgg gagtggtggg tttgtatttc ttgattccca
     8161 tgcttcgctc cttatgcctc gaattcggcg atcttgtcgc cgtccctcag ggtcacgatg
     8221 gcttttttcc aatccggccg tttgccgctg tagcgtccca aacgtttggg cttgcccagc
     8281 atccggatgg tattgacgtc aagcactttc accgaaaaga tcttttcgat ggcgtgcttg
     8341 atctctatct tattcgcgtt gatgctcacc ttgaaggaat aggtgttggt ccgttctatt
     8401 tggtttgaac tcttctcggt gatgatcggg gaaatgatga tgttgcgcgg atggatcatt
     8461 tgctgaatac ctcctcgact ttgttgaggg cttcctgggt catcaggata tagctgctgt
     8521 tcaggatctc ataggcgtaa aggctgtcgg cggcgtcggt gatgacgtcc ggcaggttgg
     8581 cgaaggattt caccgtgggc tggtgatgtc cgtcggtgac caccagtttg cggcctcttt
     8641 cggggatcat tttgcccagc agttcaagcg cctttttggt gttcggggtg tcaaattcca
     8701 gcttgtccac gatgaaaacg cggccttcgc gggccctgtc ggtgagggca accttgaggg
     8761 ccatgcgctt cttggtgcgc ggaatggtgc ggtaccaatc cttcggcagg atcgcgaaag
     8821 cgcggccgcc gtgaacccgg accggggtgc ggcgtgtgcc ctggcgggcg ttgcctgtgc
     8881 ctttctgctt gaacagcttg cgggtgctgc cggcggtcat ggcgcggttt ttcttctgca
     8941 ccgtgccctg gcgttggttg cccaggtaca tggtgataac ctcatgcagc agcactttcg
     9001 gggagttgac gtccacgtcg aaaatgttgg ccggaagttc tatctcttcc atccgttcgc
     9061 cgagggagtt gaattttatt gctttaacca tttctgctcc cttagtgttc cttgtggatg
     9121 gtgaccaggg aattgcggtg gccgggcacc gcgcctttca ccatgatcag gttttgttcg
     9181 gcatccactt tcacgacctg aagatggcgc acgctcacct ggcggttgcc gtgctggccg
     9241 gccatcttct tacctttaaa tacgcgcgag ggttgggcgc actggccgat cgagccaccg
     9301 ccgcggaagg attcgtgcac accgtgtgaa gccatgaagc cggcaaatcc gtgccgcttc
     9361 atcacaccgg tgtagccgcg gcctttggag ttggcgcgca cgcagacggt ctcggcagtt
     9421 gtgaaccagt ccactttcag ttcgctgccc acctcgtagt catcgacctg ctggccgaag
     9481 gcggggcgga attcgcgcag atagcggaaa aaggggctgt cgtttttctt gaaataaccc
     9541 ttgagcggtt tcttgacgcg cttctcggga agttcttcgt aacccatttg cagggcttca
     9601 tatccgtgct gatccttgct gcgcttgcaa accacgcggc aggggccagc ctggatcacc
     9661 gtgacgggga tcagcttgcc attgtcatca aagatctgcg tcatgccaat tttcttaccg
     9721 ataagtccca gcatgatcag ctccttgtat tggctttgat ctccacatgc acgccagccg
     9781 gcaggctcag cttcttcaaa gcgttcgtgg tctgctgggt ggggttcatg atgtcgatca
     9841 gacgcttgtg gaccagcatt tgaaactggt cttgtgattt cttgtcggcg tggggcgaac
     9901 gcaggatcgt atatagggtc ttgtctgtgg gcaacgggat cgggccgatg accttggcgc
     9961 cggtattgcg ggtgcttttc acaatttccg acatagagtg gtccagtaaa cgatgatcgt
    10021 aggccttaag ctttatccgg atgcggtttt cggatttact cactggttcc tccacaattt
    10081 aagcagaaaa tccctgtcgg gcttatgcct ccctgtgcag ggaagcagcg ccgcgtgcgg
    10141 gcgtgttctg ttaaatatgc tttcaggttg gcggggcaca aagctcttgc ttttgcccct
    10201 gcgatactcg tacgagtatc ccgtaaacag gataacatcg ttcctcctaa agaactataa
    10261 gtcctcgcaa aatatgggac ttgtgctatg cgctgggtgt atttttcgca agtgacgttc
    10321 acgatttctg ccgctccgtt ttctgtcaat agcttttttt ggctctcttt caaaccgggt
    10381 gggtagcccg gttgatcaag cccgtagggc gacataacga tagcgaggga gcataagccc
    10441 ctcgtacagg agcaataacc aggtggaaag ccccttgggg gcgacagaaa tcaatcccca
    10501 tggttctgtc gcccacaagg ggcttagaag gtagttggga tctattgtcg aggggttctg
    10561 ttcgctgcgc tcactacaca ccctcgctat gatctgccgc cctccgggct acccacccgg
    10621 tttgaaagag agcctataaa aaaaacttga cctgatttat cgagctgaaa ataatgtgac
    10681 taaccgtttg attttacata gattgtgagt aacgagggat atcaaatacc ctcgaacaac
    10741 ctgagaaaca atgagttggg atttacacca acatttggga ctcaacaatt aacgatgtga
    10801 ttgatagcaa ggatacaatc atgaaagttc gagaacttac tatagctatt attttaatat
    10861 gttttttttc ttgcagagcg tatgcgctaa gcatccaaga acagataagc caatcaacga
    10921 agctcgattt ccaaaacggg atcatgggca atgttgacga taattttagc ccctgcaggc
    10981 tgaatacgga atttgaattc tatgcagatg gccggaatct atacatgatt tgcgccgttg
    11041 agattgactc cacattcaat ttgggtaatg agactaaaag ggatcagtcc gggagtggag
    11101 attttatcat gcttcggctg ataaccgttc ccaatcaaaa ctttgcttac acctatgttt
    11161 tttatccgct tatgaacaaa gaggattaca ctctgggact gggtttaacc caaaactacg
    11221 agtggaattc agattatgaa tatgagtctg taatcaacca ggacaaatgg actatctatg
    11281 ccagaatacc ctggaaagat atgcgaacga cgaaagacac gccgtttata gtgggtgtaa
    11341 tcgcgaacag gtggatgcgc aatgagggca gggggtttaa ttatccccat gtggttaggt
    11401 cgatgggaga ggcttattat caaggagcgg ctccacttac tgtccctgtc cgcattgcaa
    11461 gggattatgg cctgaatgca agactctact acaccgcaaa atatgatctg ctaaacaatc
    11521 agaactataa agtaaaggac tatattgggc ttgatttcgc gttccgtccc cagcagaacg
    11581 gcagtctcaa actaaccttg cagccggact attcggatat tcctttggat tcggaggatg
    11641 atatctacaa tacgctttac aaaccatggc tacaggaaaa caggttcttt ttcaaggaag
    11701 acttggattt gctgaccggg tccaactcat actggtatag caggaatatc aactcgccaa
    11761 tcgccgctat aaagtattct ctcatccaac ccaagacatc ccttatcgta ctcagtgcta
    11821 aagatcaaaa gaaacagctc tataacggca cggatgactt ctacaacgcc ctggccgtca
    11881 ggcaaaaaat cgggaaaagc cagctttcac tttatgccta taacagaatg gataaaaaag
    11941 cggcatacaa taatgaggtg gcaagtataa tggtttcccg ccaattctgg gggaatcatc
    12001 tgctcagcct tgaactgggt ggcagcgtaa ccagagacag cctgccggca gattactctt
    12061 ccggatcgtc tggtattttg aaatacggct ttagcaacat caaacacaca ttagacctaa
    12121 gcggcaagta tatcgatgac tcatttcggg ctgatatggg caacatatcc ctgaagaacc
    12181 ggaacacatt taacgccacc tatacgaact atctcaattt tcccaggtca ttcatttccg
    12241 atttctcaaa ttccttgacc gctgaatatg tatccgacca taatactaat gaccgcatag
    12301 aagcttatct ggcattccgc aaccagtcca agattctttc cacccgggca tattttggca
    12361 ttgatgcttc ctatggggat gaaacatata acgatattcg atacgagtat aagaaagcat
    12421 tcctcagcct gggaagcaac tacttatcga atatctctcc ttttataagg cttggagggg
    12481 cgagaagcct ggtctatcgc ttggagaaat tggtcaattg ggcctacctt tatggctctt
    12541 tggaatcact gatcaacccc aatacggctg tgaaactcga tgctacatat tacaaatatg
    12601 accatcccaa aacggaaaac tgggataacg aatacctctt cgcgaatctt gattacgcca
    12661 tcaacttcaa cactggattt aggttcaata tggggctgag attcaataac atagaaatcc
    12721 ctgccgctat gtatgacggg tattttggat actatttcaa ttcagaatgg caggtcaata
    12781 ggatactacg ggtcaacctg ggatttcaaa gcaaggcaga tcactattcc tacagtattg
    12841 accccaatcc aacaatctac catttgtatg atataaccag tcaaaacatc tacctaaaaa
    12901 cgagtattag tttctaaact gggacagatt ataaagaatc cagtgtacac taggtgctta
    12961 cgctccctca caatcattat ttcgctgtac gtgctcgacc caccggatta tccactcagt
    13021 ttgaaagaga gccagttttt atgtccacag ataatgctat atctcttcca aactgggtga
    13081 gtaaaaccag gggacaatag aaatgcttac ccatcgtgat ttcctgccag tcacgtcctg
    13141 agcgctcttg tccatcccgc tgccatgcct ctcgtataat actcgcatca agtgcgggta
    13201 tcatgcggga ggcgtgactg ggacgcgggt cagggcctta acggccagat agcaagccac
    13261 acgctggcgt cgagcgagga acgagcatcg acgccagcaa caactcctgt agtccaaact
    13321 cgcaaagctc gctggactcc aggctttctt tcaaaccggg tgggtagcct ggagggcggc
    13381 agaaccgtgg ggtttacatt ctttcgcctg caaggggctt gccagcttgt cgtttccctt
    13441 aacaacatcc tacccgcccc aaaacccacg tcattccggg cttgacccgg aatccattgc
    13501 atgtcccgtc atagatcccg gatcaatgtc acctcgcggg ctcggtaacg tccgggatga
    13561 cgtatgttcg gttcttccgc caatgtcttg cacgtcattc cgagcttgac ccggaatcca
    13621 gttcttatcc cgttatggat cccggatcaa agtcacctcg cgggctcggt gacgtccggg
    13681 atgacgtatg ttcggctctt ccggccaatg tcttacacgt cattccgggc ctgacccgga
    13741 atccagtttt atctattcat ggaacccgga taaaagtcac ttcgcgggct cagtggcgtc
    13801 cgggatgacg tggccctgga ggctggcgtc gaacgaggca cgagcatcga cgtcagcaag
    13861 gaatgattat tccaatgcct ccttttgtcc atgctcacta cattcgctgt acgccaggct
    13921 tcaaggaaaa tggcggtaca gcgaccgcct ggacataggt agcggcgctc ggctttacca
    13981 atccggtttg aagagagccg aataagagcc caaaaaaagc agcggaggaa gatatccatc
    14041 cgctgcaatg ttaacaggcc aacaggcaac taagcgcgcc aagcctgatt gatgtccagg
    14101 tattatttca tcagcagcat tctgcgggtc tggctggctc cggcggtttc cagtttgtag
    14161 agataaaccc ccgaacttac ggactttccg gcttcgtcga gtccgttcca gacaacgctg
    14221 tgcttgccgc cgggaagttc tgaatccacc agggtcctga tcagttggcc cttggtgtta
    14281 tagacagcca ggcgtgcctg accgctttgg gcgaggctga aggaaagggt ggtgctggga
    14341 ttgaagggat tgggataatt ctgttccagc gcgaaaggca cggccaccgg atcgttcgcg
    14401 gccacatagc tgtccggggc cagggtggtg aattttatgg ccagacctgc ctgcaggggt
    14461 gtggctgtgg gcggatagac gttgccgtga gtgtagccga ggccaactgt ctgggtgtgg
    14521 ttttcgatgc ccactgtgca gtaattggtg gtgaggccgg gattgtccac ggtgtggtac
    14581 tggaccacga tgtcgccgtc acggtcggcc tggggataga ggatgatctg gaatttctcc
    14641 agggaggcgt tttccatcag gtctatgttg tattggttgt aggcgtcgtt ccattgcacg
    14701 atgtagcggt tgttggcggc gtcgtgccag cggatcaggc gcatgttgct gaaggtgttg
    14761 tcgccggttt tcatgccctt gaggtcgtcc caatagccgg cgatcatggc ataggggccc
    14821 agcgcggcgg ggatgtagca gttgtagaaa tccaccatgt cggtgggcag cagcgagatc
    14881 cagccgtttg agcacatggt tacgtgattg tattcgcggc cgtagaagcg gaaactgaaa
    14941 ggcaggtcaa ccgtgagcga accgtcgtcc atcacctccc aaaccgtccc ctgcccgccc
    15001 aaggcgggat cggtctcgat ccagttgtaa accggggttt ggacaaagcc ggcatcgaag
    15061 ctgtcatagg cgaaatagcc atattcgtca ggtccggtgg gagaagtggt ggccggcgtt
    15121 ccggcggtga ggctatagaa agcggtgttg gaatatccgt ttgagctcac caccattttc
    15181 atgggcaggt tgcggccatg ccaggcttcg gagcccaccg tgatatcgac gctgaatgtc
    15241 ttggtctcgc cggggccgaa gttgcccagg ttcgcgcttc cgccgttctc aaagtt
//
LOCUS       DAOOWR010000006        13554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_4568_length_13554_cov_4.088229, whole genome shotgun sequence.
ACCESSION   DAOOWR010000006 DAOOWR010000000
VERSION     DAOOWR010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_13554_cov_4.088229"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="PLB85_00430"
     CDS             complement(<1..1490)
                     /locus_tag="PLB85_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU71822.1"
                     /translation="MFKSIDLKENPRHLEERIRAYWREHSLAQKSIDLRKGSPQFVFY
                     EGPPTANGKPGIHHMLSRTLKDLVCRYKTMNGFQVKRKAGWDTHGLPVEIEVERQLGL
                     EDKKAIEDYGVEKFCQACKESVWSYLDLWQEMTELMGYWIDLDDPYITMHNSYIESVW
                     HILDDFFRRGLIYKAHKIVPYCPSCGTPLSSHEVALGYRDVEDPSIFVKFKALDEDGT
                     WYLAWTTTPWTLISNVALVVHPDETYVKVRHLEENYILAKARLETLEGEYEILSEFPG
                     RELERRRYQPLFSFIKPEKPAWYVGLADYVSMDDGTGIVHTAPAFGADDYALGLKYDL
                     PFIQPVDAEGKFNHLIEPWAGIFVKTADKDIIRSLKESGNLYRREQVKHSYPHCWRCE
                     NLLIYYARESWYIRTTQFKEQLLEQNRQIDWYPGFVGEKRFGEWLANNVDWALSRDRF
                     WGTPLNIWLCDRCGAQHLPVSRAELGELTGRDQSGLSVQDRGGGVQL"
     gene            complement(1570..1935)
                     /locus_tag="PLB85_00435"
     CDS             complement(1570..1935)
                     /locus_tag="PLB85_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71823.1"
                     /translation="MFKLDDKHLEAAQQIAAKHRRKKSCDNCYDRGWIGTTDQNLLVL
                     CTRCVNMDAAMEEWKTYVSEHEELKEHFSELFEEKQVTEAEEERVLPTAHEHKKTNLQ
                     ARQNFVPGQRRPGHSKKIG"
     gene            complement(2073..2573)
                     /locus_tag="PLB85_00440"
     CDS             complement(2073..2573)
                     /locus_tag="PLB85_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71824.1"
                     /translation="MNKSRLHACEIKRHREVTPPLCAVCRRVGKCRAFRIWYQSHRDE
                     YLDFVLDICRRFPDKYTMEVDFMAEKQTFVQIVDMATGAIERIVNLKEIVAMTPEEKL
                     ELSRNKNLFVVTHRLEPIVRVELKRSVITEPLQFAENTAAKAAPEAAENVEPAPPAAS
                     KTRKKK"
     gene            2862..3002
                     /locus_tag="PLB85_00445"
     CDS             2862..3002
                     /locus_tag="PLB85_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71825.1"
                     /translation="MKDNSTEMFINDSNSAFSRSRVSFGLNGKSRRRATPSPDSILLA
                     LE"
     gene            3011..3931
                     /locus_tag="PLB85_00450"
     CDS             3011..3931
                     /locus_tag="PLB85_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOU71826.1"
                     /translation="MRAVIDIGSNSVKCVVAQGVGTDFQVIRELSRTTRLGEELVVTG
                     MIGPEAAERSLAYLRELSDLCRDYGVKSVVCVGAETLRRASDAEQFARRLNCLTGWEL
                     RVLTPEGEARLSFEAASALAPTDGDFVVIDSGGGSTEFSFGSKQGLKTCHSLPLGALT
                     LTSSFIHGDPPGPEELSYLRNHVRGLLQSEFPRPGKLHAVACGGGVCALAAVAMELED
                     YEAEKVQGFWLSQTELERQIVLYGSVTEAERARIKGLPPGREAIILAGALVLEEILLH
                     FELKGTSISSRGIRHALLAEIDPIWFMPAT"
     gene            complement(4131..4206)
                     /locus_tag="PLB85_00455"
     tRNA            complement(4131..4206)
                     /locus_tag="PLB85_00455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4171..4173),aa:Ile,seq:cat)
     gene            complement(4381..5148)
                     /gene="rnc"
                     /locus_tag="PLB85_00460"
     CDS             complement(4381..5148)
                     /gene="rnc"
                     /locus_tag="PLB85_00460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU71827.1"
                     /translation="MKKIIHRIVEYFNGKKIAEHYPKWEKSLAQLQKKIEYNFHDPTL
                     LRAALTHKSYLRRNFGDHKTPSPFERMEFLGDSILGFIVSKELFSRHPDEQEGKLSKL
                     KSKIVSETYLTLKANALDLGKYLLLSPEEQQSGGAKKSSILSDSVEALICAIYLDSGI
                     ASATRFIKNQILTDYETTVNRNELVNYKSILQEHLQAHSEEPPRYVTVAEEGPEHNKT
                     FIVETRLGNELLGRGKGNTKKTAQQEAARAACQKLGL"
     gene            complement(5210..6448)
                     /gene="fabF"
                     /locus_tag="PLB85_00465"
     CDS             complement(5210..6448)
                     /gene="fabF"
                     /locus_tag="PLB85_00465"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU71828.1"
                     /translation="MKRRVVITGMGALTPVGHNVAETWNNLVNGVSGVDTITSFDTSA
                     LSAKIAAQIKDFHPEEHFDLKEARKMDSFTHFAMVAAREAVKDAGIDSARQDGRRVGV
                     ITGVGIGGIYTFEEETIKNHLQGPRRISPFFIPKMISNIAAAHIAIEHGFKGPNFSIA
                     SACASANHAIGTAFRAIQYGDADIIISGGAEAGVTALSVGGFCALRALSTRNDDPKGA
                     SRPFDAGRDGFVMSEGAAFLVLEELEHAKARGATIYAELAGYAATDDAFHITAPLDDG
                     SGSAEAMLGAIADAGISPADIQYVNAHGTSTPLNDKGETYSLKSCFGDHAYKLKINST
                     KSMVGHMLGAAAGIEAIVCVKTIETGILHPTINLSQPDPDCDLDYVPNTPQNHEVIYA
                     LSNSLGFGGHNSALVIKKYI"
     gene            complement(6582..6815)
                     /locus_tag="PLB85_00470"
     CDS             complement(6582..6815)
                     /locus_tag="PLB85_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU71829.1"
                     /translation="MDIEAKVKQIVMDKLGVEESQVVPAANFIEDLRADSLDTVELVM
                     AFEEEFSINIPDEDQDKLRTVGQAIDYLKEKLA"
     gene            complement(6881..7639)
                     /gene="fabG"
                     /locus_tag="PLB85_00475"
     CDS             complement(6881..7639)
                     /gene="fabG"
                     /locus_tag="PLB85_00475"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOU71830.1"
                     /translation="MNTANYNLQNKTVVITGAARGIGFAIAEAFAVQGSHVVIVDLAR
                     DSVDQAVQSLTQRGFSASGEVGDVTQGEAMEAIFASIVSVRGGIDCLVNNAGVTRDNL
                     LLRMKQEEWDLVLKINLSGSFICTQKVFKHMMRARSGSIINIASVIGIIGNAGQANYA
                     ASKGGLIAFTKSCAKEFASRGVRVNAIAPGFIETEMTATLPAEVVASYAKAIPLQKMG
                     SPADIARVCLFLASDDSSYLTGQTIAVDGGLTMH"
     gene            complement(7662..8591)
                     /gene="fabD"
                     /locus_tag="PLB85_00480"
     CDS             complement(7662..8591)
                     /gene="fabD"
                     /locus_tag="PLB85_00480"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU71831.1"
                     /translation="MKTAFVFPGQGAQYVGMARDFCEAVPEFAIELEAFDRCHGTDLR
                     QIMFEGPEELLKETRHTQPAILFHSICALKVFERHTNIRPACVAGHSLGEFSALVANG
                     TLDWQDALHLVHKRGEFMIEANAGSPFAMAAVIGLEAQAVIEACAEAEAEGLVRAVNF
                     NTPIQTVISGTEAGVAKAREILSAKGAKRVLPLVVGGPFHTPLIEKASHWLEEEMARV
                     EFSDAEIPVISNVDAAPAIEGSVSRVKLVRQVVSPVLWVDSVKAMLASGITRFIEFGP
                     QKVLRGMIKNIDKEAKVLNFGRMEELDDLLSAL"
     gene            complement(8602..9594)
                     /locus_tag="PLB85_00485"
     CDS             complement(8602..9594)
                     /locus_tag="PLB85_00485"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU71832.1"
                     /translation="MPLHNAKFASFGSHAPAKIVNNFDLEKIVETSDEWIRTRTGMFE
                     RHHCTPEEAASDLAIVAATNAIEASKVRYRDIDLIVTATVTGDHPFPSTSCVLQKKLG
                     LKNIPAFDVSAGCTGFVYALDVAKNYVENGSAQNVLVIGVDILTKITNWSDRNTCVLF
                     GDGAGATILSRAQKNDISRIIDSLIFADGSQGELLYQAAGGSRMPASHESVDKNLHTV
                     YMEGNRIYKNAIRSMFASTDELLKRNKMGVEDVDWIIPHQANLRIIEGLADKLHVPMS
                     KVIVNIEKYGNTSSATIPLAMDEAIRANKIRRGDIILLTSFGAGLTWGSLLARY"
     gene            complement(9537..10592)
                     /gene="plsX"
                     /locus_tag="PLB85_00490"
     CDS             complement(9537..10592)
                     /gene="plsX"
                     /locus_tag="PLB85_00490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOU71833.1"
                     /translation="MRIALDAFGSDKAPFPEIEGAIQAIKEDICDEVILVGDEASLRN
                     DLEKYFYDKDRILIEHASERINMEDHAAEAVRAKKNSSMVRSVQLHMSGAADAAVSAG
                     NSGAFMTASLFGYGRIKGVSRPAIAVTLPTMKQPEILLDMGANVDCDAENLLQFAQLG
                     TIYFSYIYKVPNPRVALLNIGEENAKGNFMSREAWNLLSQAQDLNFVGNIEGKDVLKG
                     DCDVIVCDGFVGNVVLKTIEGAVMAVFELLKEQLNKDWIAKFGAVLSYPVYSYLRHKL
                     DHTEIGGALLVGLNGCSIVCHGSSNANAIKNGIQFAVRTAQAGVVNHTKEYFERISKC
                     HCTMPNSLLSAATLRQR"
     gene            complement(10594..10779)
                     /gene="rpmF"
                     /locus_tag="PLB85_00495"
     CDS             complement(10594..10779)
                     /gene="rpmF"
                     /locus_tag="PLB85_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011702823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU71834.1"
                     /translation="MAVPKRRTSKTRRDKRRTHDALTPPAVSTCSKCGEPARPHHVCD
                     NCGTYSGRQIKAAAEKE"
     gene            complement(10964..12340)
                     /locus_tag="PLB85_00500"
     CDS             complement(10964..12340)
                     /locus_tag="PLB85_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU71835.1"
                     /translation="MNDRSGKEHKAQQKSFTVFRTYRISVIEGVFAQIYGNLANIGSN
                     FITKFMVILGASPLQFSILSALGQVAAVFQPLGVALMQRLKRRKRVCVAVTAAGRFLS
                     FFLGAALLFSSSQAGIVFLLALLFVSAGLQAVGGNIWIAWVSDLIPIGIRGRFFARRN
                     QILVFSGMVVGYILSYFTDLFEKGGGALRNLTSQWVDPDILFTPRNQAWWLAGIFVFA
                     GIMGFISLAILSRQPDRKRSLQQQMSLRRKFSEPFRDRNFRMLLAFGVWWMLAIGVGS
                     AFWGPFMLKKLNMGLFQMQLYGSLHMGSSLLSYSFWGRFIDRWGNKRAMFICVLLGGL
                     NPMLWLFMTPANYSILWLEGLLSGFMWAGNGVVTTNFVLAIAGKGREQTYSALYGAVG
                     GVAMMASTLLTGAFFPGALRIGARLLEPEQVIFGVGGILRWLSLVPLLAVKEKRGWTQ
                     WHRSPGSR"
     gene            12828..>13554
                     /locus_tag="PLB85_00505"
     CDS             12828..>13554
                     /locus_tag="PLB85_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOU71836.1"
                     /translation="MFNKAVLLMILLALACVAGAEDWITIYNDDLSLVRSNFELELQA
                     GRQDYNFDNITSRIEAPSVIVHSLDGGVRVAEQNYEYDLAGKYQIIAKYIEREVELLT
                     KDDSRVIGTLKFYDGSSFGILEKGSSRLLVVADSETQIIKLAELPSNFYTKPTLHWSL
                     IADKAGRHPLQMTYLTGGLNWNVTYNSVWDENNLALNSWVTITNQSGKAFDDVTLKLI
                     AGDVNRVYDVYNGYKRTYEEMMAP"
BASE COUNT         2750 a         3676 c         3931 g         3197 t
ORIGIN      
        1 agctgtacac ctccaccccg atcctggacg ctgaggcccg actggtcgcg gccggtcagc
       61 tcgcccagtt cggcgcgcga gaccggcagg tgctgcgcac cgcaacggtc gcacagccag
      121 atgttcagcg gcgtgcccca gaaacggtct cgggaaaggg cccagtcaac gttgttggcc
      181 agccattcgc cgaagcgttt ttcgcccacg aagccaggat accagtcgat ctggcggttt
      241 tgctccagca gttgttcctt gaactgggtg gtgcggatgt accagctttc gcgggcatag
      301 tagatgagca ggttttcgca gcgccagcag tggggatagc tgtgtttaac ctgttcgcgg
      361 cggtagaggt tgccgctttc cttcaagctg cggatgatgt ctttgtccgc ggttttcacg
      421 aagatgccgg cccagggttc gatcagatgg ttgaatttgc cttcggcatc caccggctgg
      481 atgaaaggca ggtcatattt gaggccgagg gcatagtcgt ccgctccgaa agccggggcg
      541 gtgtgcacta tcccggtgcc gtcatccatg ctcacgtaat ctgccaaacc aacataccaa
      601 gcgggttttt ctggcttgat gaaactgaaa agcggctgat agcggcgccg ttccagctcg
      661 cggccgggaa attcgctcaa aatctcgtat tcgccttcca gagtttccag ccgggctttg
      721 gccaggatgt agttttcctc cagatggcga accttcacgt aagtttcgtc aggatgcaca
      781 accagtgcca cattcgaaat cagcgtccaa ggcgtggtag tccaggccag ataccaggtg
      841 ccgtcttcgt ccaaagcctt gaatttcacg aagatagatg gatcctcaac gtccctgtag
      901 cccaacgcca cttcatggga agagagcggc gttccacagc ttgggcagta aggcacgatc
      961 ttgtgcgcct tgtagatcag cccgcggcgg aaaaagtcgt ccaaaatatg ccacacgctc
     1021 tctatgtagc tgttgtgcat ggtgatgtaa ggatcgtcga ggtcgatcca gtagcccatc
     1081 agttccgtca tttcctgcca gaggtcgagg tagctccaga cagattcttt gcaggcctgg
     1141 caaaattttt ccaccccgta gtcttcgatc gctttcttat cttccagtcc cagttgccgt
     1201 tccacttcga tctccaccgg cagtccgtgg gtgtcccagc cggctttgcg tttaacctgg
     1261 aaaccgttca ttgttttgta gcggcacacc aaatccttca gcgtgcgtga cagcatgtgg
     1321 tggatgcccg gtttgccatt ggctgtggga gggccttcat aaaacacgaa ttggggtgag
     1381 cccttgcgca aatcgatgct tttttgggcc agggagtgtt cccgccaata ggcgcggata
     1441 cgctcctcca gatgcctggg gttttcttta aggtctatgc ttttgaacat ttaaactaaa
     1501 attgcgtcct tgagttaagc tggccctggt tgggcctgat tatatggccg gaagacgcgg
     1561 aatcttcact cagccaatct tttttgaatg tcccggacgc ctctggccgg gaacgaaatt
     1621 ctgcctggct tgcagattgg ttttcttgtg ttcatgggcc gtggggagca cacgctcctc
     1681 ttccgcttcc gtaacctgct tttcctcaaa gagttcgctg aagtgctcct tcagttcctc
     1741 atgctcagaa acgtaggttt tccattcttc catggctgca tccatgttca cgcatcgggt
     1801 gcagaggacc agcaggtttt ggtctgttgt gccgatccag ccgcggtcgt agcagttgtc
     1861 gcagctcttt ttgcggcgat gctttgccgc gatttgctgg gctgcttcca gatgcttgtc
     1921 gtcaagcttg aacataatgt ctccttttcc ccggtcagcg cttgcctttt gggcaagtac
     1981 agccgcggga tgctgttgtt tacagccagt tataccatta tttttaccgg cgcatttctg
     2041 aaaaggatta ttttcagccg gctggaaggg cgctatttct ttttgcgcgt cttgctggcc
     2101 gcaggcgggg ctggctcgac gttttcagcc gcttccggag cggctttggc agccgtgttt
     2161 tccgcgaact gcagcggctc tgtgatcacc gaccttttca gctccacccg cacgatcggt
     2221 tccaggcggt gggttacaac aaagagattc ttgttgcggc tgagttccag cttttcttcc
     2281 ggcgtcatcg ccacgatctc tttcaggttc acgatccttt cgatcgctcc ggtggccatg
     2341 tccacgattt ggacaaaggt ttgcttttcg gccatgaagt ctacctccat tgtgtattta
     2401 tcaggaaatc ttctgcaaat gtcaagcaca aaatccagat actcgtcgcg gtgggattga
     2461 taccagatgc gaaaagcgcg gcatttcccc acccggcggc aaaccgcgca gaggggcggc
     2521 gtcacttctc ggtggcgttt gatttcgcag gcgtggagtc tggatttatt catagctggc
     2581 cttttctctg aaaagcctct ccctgtcaag aaatatcggc gtcaacacgg caacgaatta
     2641 ccccgccttg ggcaggttca cagacttgtt atactgcgct tggagcaggt cataggcgtc
     2701 ttccctgttg cgggttagcc ttagcgcgaa attcagcaat ggtctttcat gcgtgtttac
     2761 aacaaaagcc agggcccacc acttccgcgg gtggaaaatg ctggacaaat ttggcagcgc
     2821 ctttcaagag gcttaaattc aaattacaga caggtccaaa catgaaagat aactccacgg
     2881 agatgtttat caacgattcc aacagcgcct tcagccgcag ccgtgtcagc tttgggctga
     2941 acggcaaatc cagaaggcga gccacacctt ccccagattc gattttactg gccctggagt
     3001 gaacaatcag atgcgggcag taatcgatat cggtagcaat tccgtcaaat gcgttgtggc
     3061 gcagggagtt gggactgact ttcaggttat tcgtgaattg agccggacca cccggctggg
     3121 tgaagaattg gtcgtcacgg gcatgattgg gcccgaagcg gctgagcgca gcctggctta
     3181 tctgcgcgag ctaagtgacc tctgccggga ttatggggtg aaaagtgttg tctgtgtcgg
     3241 cgctgaaacc ttgcgcaggg cgtctgacgc ggagcagttt gcccgccggt tgaactgcct
     3301 tacaggatgg gagttgcgcg ttctcacacc cgaaggggag gcgcggttga gctttgaggc
     3361 tgcttcagcg ctggctccca cagatggaga ctttgttgtg atagattccg gcgggggcag
     3421 caccgaattc agctttgggt ccaagcaggg gctgaaaacc tgccacagcc tgcctttggg
     3481 cgcgctgacg ctcaccagtt cctttatcca cggcgatccg ccgggccccg aagaactgag
     3541 ctatctcaga aaccacgtcc gcggcctttt gcagagtgag tttccccgcc cggggaagct
     3601 acatgccgtc gcctgcggcg gcggagtctg tgccctggcg gcagtggcaa tggagttgga
     3661 agattatgag gctgaaaagg tgcagggatt ctggctgtcc caaacggaac tggaaaggca
     3721 gattgttctc tacggcagtg tcacagaggc ggaacgcgct cgcataaagg gtttgccgcc
     3781 tggcagagaa gcgataatac tggccggggc actggttctg gaggaaatcc tgctgcactt
     3841 tgagctaaag ggcacgagca tttccagccg gggcatccgg cacgctttat tggccgaaat
     3901 cgatccaatc tggttcatgc cagccacctg accctgccag ttcggacttc tcttttggct
     3961 ggaccttaac cccctttata aagccagcgt tttctctccc gcacaatccc cgcttgaaat
     4021 gcgggcattt ggagggagca atgcaagagg cggaccggaa ggggctaagg aacgactgac
     4081 gcgggatgaa ccgctttaac aacaggaaca gaccctattt caaaaaaaga tggtgggccc
     4141 agagggagtc gaacccccga ccgtccggtt atgagccgga agctctacca actgagctat
     4201 aggcccctca tcattgtcac tcctttcatt ttcaggcctt tattctgtca agcaaaatcg
     4261 gttttttatg aaaggaggaa cgagttttga atacagtaat gaacagcgct tcaaagcatc
     4321 ctggcaccca ttcccacaag ctattctgtt gcctgcgttc gctgtatagt caggatgagc
     4381 tcagagcccg agtttctgac aggcggctcg agcggcttcc tgttgagcgg tctttttggt
     4441 gttgcctttg ccgcgtccaa gcagttcgtt gcccaggcgg gtttccacaa tgaaggtttt
     4501 attgtgttcc gggccttcct cggcgacggt cacatagcgg ggcggttcct cgctgtgggc
     4561 ctggaggtgt tcctggagga tgcttttgta gttcaccagt tcgttgcggt tcaccgtggt
     4621 ttcatagtcg gtgaggatct ggttctttat gaatctggtg gcggaggcga tgccgctgtc
     4681 caggtagatg gcgcagatga gggcttcaac ggaatcgctg aggatggagg attttttggc
     4741 gccgccggat tgctgttcct cggggctgag cagcaggtat ttgcccaagt cgagggcgtt
     4801 ggctttgagg gtgagataag tttcggagac gatcttggat ttgagcttgc tgagcttgcc
     4861 ctcctgctcg tcgggatggc gtgaaaagag ctctttggaa acgatgaaac cgaggatgga
     4921 gtcgcccaaa aactccattc gttcaaaggg cgagggggtt ttgtggtcgc cgaaattgcg
     4981 gcgtagatag gatttgtggg taagggcagc gcgcagcagg gtggggtcgt gaaagttgta
     5041 ttcgatcttt ttctgcaact gggccaggct cttttcccac ttgggatagt gctcggctat
     5101 ctttttgccg ttgaagtatt caactatcct gtgtatgatc tttttcaatc ttgcctctgt
     5161 tcatccggcc agcaaatccc gccacagcgg ccctccgggg gcgcggggat cagatgtatt
     5221 ttttgatcac gagggcggaa ttgtgcccgc caaagcccag agaattggag agggcataaa
     5281 tgacctcatg attctgcggg gtgttgggca cgtaatccag gtcgcaatcg gggtccggct
     5341 ggctgaggtt gatggtgggg tgcaggatgc cggtctcgat ggtcttaaca cagacgatgg
     5401 cctcgattcc ggcggctgcg cccagcatat ggccgaccat ggatttggtg gagttgattt
     5461 tgagcttgta ggcatggtct ccaaagcaac ttttgaggga gtaggtttcg cctttgtcgt
     5521 tgagaggggt ggaggtgccg tgggcgttca catactggat gtcggcgggg ctgatcccgg
     5581 cgtcggcaat ggcgcccagc atggcctcgg cgctacctga gccgtcgtcg aggggggcgg
     5641 tgatgtgaaa ggcatcgtcg gtggcggcat atccggccag ttcggcataa attgttgctc
     5701 cgcgggcttt ggcatgttcc agttcttcca gcacgaggaa ggcggctcct tcgctcatca
     5761 caaagccgtc acgtccggcg tcgaaggggc gggaagcgcc tttgggatcg tcgttgcggg
     5821 tggaaagggc cctcagggcg cagaatccgc cgacggaaag cgcggtcacc ccggcctcag
     5881 cgccgccact gatgatgatg tcagcgtcac catactggat ggcacggaaa gcggttccga
     5941 tggcgtggtt ggcgctggcg caggcgctgg caatgctgaa gttggggcct ttgaagccgt
     6001 gttctatggc gatgtgggcg gcggctatgt tggagatcat tttggggatg aaaaaggggc
     6061 tgatccggcg cgggccctgc aggtggtttt tgatggtctc ttcctcgaag gtgtagattc
     6121 cgccgatgcc cactccggtg atcacgccta cgcggcggcc gtcctgcctg gcagaatcta
     6181 taccagcgtc cttaacagcc tcgcgggccg ccaccatggc aaagtgggtg aaggaatcca
     6241 tcttgcgggc ttccttgagg tcgaaatgct cttcgggatg aaaatctttt atctgggcgg
     6301 cgatcttggc ggaaagggct gaggtgtcga aggaggtgat ggtatccact cccgacacac
     6361 cgttcaccag gttgttccag gtttcagcca cattgtgccc caccggggtc agggctccca
     6421 tgcctgtaat aaccactcgt cttttcatat cttgatcctc ttgttcagtt tgcctgctgg
     6481 cgggttttgg gaaaagccgg cagggatttt cgcttttgga aaaagggtgg aatcaatcct
     6541 gataacataa gctatcagga ttgattcacg gttttggggg attaagccag cttttccttc
     6601 aggtagtcga tggcttgtcc gactgtgcgg agcttgtcct gatcttcatc agggatattg
     6661 atcgagaatt cttcctcaaa tgccatgacc aattccacag tgtccaggga atcggcacgc
     6721 aggtcttcga tgaagttggc ggcgggaaca acttgagatt cctccacgcc gagtttgtcc
     6781 atcacgatct gtttgacttt ggcttcaata tccatttttc ctcctatgat tattgaagtt
     6841 aggttttctt tctatattga atggtttccc gttcaacgct tcagtgcatg gtcagaccgc
     6901 catcgacggc aatggtctga ccggtgaggt aggagctgtc gtccgaggcg agaaagaggc
     6961 agactctggc gatgtctgcc ggggagccca ttttctgcaa cgggatggct tttgcgtagg
     7021 aagccaccac ctcggcagga agggttgcgg tcatctccgt ctcgatgaat cctggagcaa
     7081 tggcgttcac gcgcacgccc cgggaggcga attccttggc gcagcttttg gtgaaagcaa
     7141 tgagaccgcc tttggaggcc gcgtagttgg cctggccggc gttgccaatg atgccgataa
     7201 cgctggcgat gttgatgatg gagccgcttc tggccctcat catgtgtttg aaaactttct
     7261 gggtgcagat gaagctgcca ctgaggttga ttttcagcac caggtcccat tcctcctgct
     7321 tcatgcgcag cagcagattg tcgcgggtga cgccggcgtt gttcaccagg cagtctatcc
     7381 cgccgcggac gctgactatg gaagcaaaaa tggcttccat ggcttcaccc tgggttacat
     7441 cgcccacttc accggaggct gagaatccac gctgggtgag gctttgaacg gcttggtcca
     7501 cgctgtcgcg ggccagatcg acgatcacca catggctgcc ctggacggca aaagcttccg
     7561 ctatcgcgaa gccgattccg cgggcggcgc cggtgattac cactgttttg ttctgaaggt
     7621 tgtagttcgc agtattcata tcttcattct ttataatgtt tttagagggc gctgagcaaa
     7681 tcgtccagct cctccatccg gccaaagttt aaaaccttgg cttctttgtc aatgttcttg
     7741 atcatgcctc ttagcacttt ttggggcccg aactcgatga aacgggtgat tccggaggca
     7801 agcatggcct ttacactgtc cacccagagc accggcgaaa ccacctggcg caccagcttg
     7861 acgcggctga cagagccctc tatggcgggc gctgcgtcca catttgaaat cacggggatt
     7921 tcagcgtcgc tgaactccac cctggccatc tcctcctcga gccagtggct ggctttttcg
     7981 atcagtggag tgtggaaggg cccgcccacg accagcggca aaaccctctt ggcgcctttg
     8041 gcggagagga tctcgcgggc tttggcgacc ccggcttctg tgccggaaat cactgtctgg
     8101 attggggtgt tgaagttcac cgcgcgcacc aggccttcag cttcagcttc ggcgcaggct
     8161 tcaattacgg cctgggcttc cagccctatc acggcagcca tggcaaaggg gctgccggcg
     8221 ttggcttcta tcatgaattc cccgcgtttg tgcaccaaat gcagagcgtc ctgccagtcc
     8281 agggtgccgt tggcgaccag ggcgctgaat tcgcccaggg aatggccggc cacgcaggcg
     8341 gggcggatgt tagtatggcg ctcaaaaacc tttagggcgc agatgctgtg gaagaggatg
     8401 gccggctggg tgtggcgggt ttccttcaaa agctcttccg gtccttcaaa catgatctgg
     8461 cgcagatccg tgccgtggca ccggtcaaag gcctccagct cgatggcgaa ttcgggcacg
     8521 gcttcacaga aatcacgggc catgcctacg tattgggccc cctgtccggg aaaaacgaac
     8581 gctgttttca tactgtggtc ctcaatacct ggccagcagg ctgccccaag tgagcccggc
     8641 gccgaaggaa gtgagcagaa tgatatcgcc gcggcggatc ttgttagcgc ggatggcttc
     8701 gtccatcgcc aggggaatgg tggccgagga ggtgttgccg tatttttcga tgttcacgat
     8761 caccttgctc atcggcacgt gaagtttgtc agccaggcct tcgataatgc gcaggttggc
     8821 ctggtggggg atgatccagt cgacgtcctc aacccccatt ttgttgcgct ttagcagttc
     8881 gtcggtggag gcaaacatcg aacggatggc gtttttgtag atgcggttgc cttccatgta
     8941 aacggtgtgg aggttcttgt ccacggattc gtggctggcg ggcatacggg aaccgccggc
     9001 tgcctgatag agcagttcgc cctggctgcc gtcggcaaaa atgagtgaat caatgatcct
     9061 tgagatatcg ttcttttggg cccgggaaag gatggtcgcc ccggcgccgt cgccgaagag
     9121 aacacaagtg ttgcggtcgc tccagttcgt gatcttggtg agaatgtcca cgccgatcac
     9181 cagcacattt tgcgcgctgc cgttttccac gtagtttttg gccacgtcca gggcgtacac
     9241 aaagccggtg cagccagcgg agacatcaaa ggcggggatg tttttcaggc ccagcttttt
     9301 ctgcaacaca caggaagtgg agggaaaggg atggtcgccg gtcacggtgg cagtcacgat
     9361 gaggtcgata tcccggtatc tcactttgga agcctctatg gcgttggtgg ctgcgacgat
     9421 ggcaaggtca ctggccgctt cctccggagt gcagtggtgg cgctcaaaca tcccggtgcg
     9481 ggtgcggatc cattcgtcgg aggtttccac gatcttttcc agatcgaagt tgttgactat
     9541 ctttgccgga gcgtggctgc cgaaagaagc gaatttggca ttgtgcaatg gcatttgctt
     9601 atcctctcga aatattcctt ggtgtggttc acaacacctg cctgcgcggt gcgcacggcg
     9661 aattggattc cgttttttat agcgttggcg ttgctgctgc cgtggcaaac gatgctgcag
     9721 ccgttcagcc ccaccagcag ggcgccgccg atctcggtgt ggtcgagctt gtgccttaga
     9781 tagctgtaaa ccggataaga gaggaccgcc ccgaatttgg cgatccagtc tttgttgagc
     9841 tgctctttca gcagttcgaa aacggccatc accgcgcctt cgatcgtctt cagcaccacg
     9901 ttgcccacaa agccgtcgca gacaatcacg tcgcagtctc ccttcagcac gtccttgccc
     9961 tcgatgttgc ccacgaaatt gaggtcctgt gcctgggaaa gcaggttcca ggcctcacgg
    10021 gacataaaat tgcccttggc gttttcctcg ccgatgttca gcagggcgac gcgggggttc
    10081 ggaaccttgt agatatagct gaaatagatg gtgccaagct gggcaaactg gagcaggttt
    10141 tcggcgtcgc aatccacgtt cgcgcccata tccagcagga tttcgggctg tttcatagtg
    10201 ggcagggtaa cagcgatcgc gggacgggaa acgcctttga tgcggccgta gccaaagagg
    10261 gaggcggtca tgaaagcccc ggaattgccg gcgctcacag ccgcgtccgc agccccggac
    10321 atgtgcagtt gcactgagcg gaccatcgag gaattctttt tggcgcgcac tgcttcagcg
    10381 gcgtggtctt ccatgttgat gcgctcgctg gcatgctcga tgaggatccg gtctttgtcg
    10441 tagaagtatt tctccagatc attccggaga gaggcttcat cgccgaccag gatgacctcg
    10501 tcgcaaatgt cttccttgat ggcctgaatg gcgccctcta tctctggaaa cggcgctttg
    10561 tcgcttccaa aagcgtctag ggcaatgcgc acattactcc ttctcggcgg cggctttaat
    10621 ctgcctgccg gagtaggtgc cgcaattgtc gcacacgtgg tgcggacggg cgggctctcc
    10681 gcatttggag caggtgctca cagccggggg cgtgagcgcg tcgtgcgttc tgcgtttatc
    10741 tctgcgggtt ttcgaagttc ttctttttgg gactgccatc gggtactcct tttggttgtg
    10801 tcttattcag gcttcagccc ttgcccgggc aggccgaaag tgcctcgcgg gaaccaagct
    10861 gaagcaaaat aatgggattc gagcaaactc tgccagcatc ggtattttgt caatcaaaat
    10921 ccccaactca atgactttca accaaatata gcctgagtgc cgctcacctg gaaccagggc
    10981 tccgatgcca ttgcgtccag ccgcgctttt ccttaaccgc cagcagcggg accaggctta
    11041 gccagcgcag gatgcctccg acgccgaaaa tcacctgttc cggctcaagc agcctcgccc
    11101 ctatccgcaa agcgccgggg aaaaaagctc cggtgagcag ggtggaggcc atcatcgcca
    11161 cgccgccaac ggctccgtag agcgcgctgt aagtctgttc gcggcccttt ccggcgatgg
    11221 ccagcacaaa attggttgtc accacgccat tccccgccca catgaaaccg gaaagcaagc
    11281 cctcgagcca gagaatgctg taattcgccg gggtcatgaa cagccaaagc atggggttca
    11341 atccccccag cagcacgcag atgaacattg cgcgcttgtt gccccagcgg tcgatgaagc
    11401 gtccccaaaa ggaatatgag agcagcgatg agcccatgtg caggctgccg tagagctgca
    11461 tctggaagag gcccatgttc agttttttga gcatgaaggg accccagaag gcactgccca
    11521 cgccgatcgc cagcatccac cagaccccga aagccagcag catcctgaaa ttgcggtctc
    11581 tgaaaggttc gctgaacttg cgccgtagcg acatctgctg ctgcagggaa cgcttccggt
    11641 ccggctgccg cgacaggatc gccagactga taaagcccat aatccccgca aacacaaaga
    11701 ttcccgccag ccaccaagcc tggttgcgcg gagtgaagag gatatccgga tcgacccatt
    11761 ggcttgtcag gttccgcagc gctcccccgc ctttttcaaa gaggtctgtg aagtagctca
    11821 ggatatagcc caccaccatt ccggaaaaga ccaggatttg gtttcttcgc gcgaaaaagc
    11881 ggccccggat gccgatagga atgaggtcgc tgacccaggc gatccagatg tttccgccca
    11941 cggcctgcaa gcccgcgctc acaaaaagca aggccagaag gaaaacaatc cccgcctggg
    12001 atgaggaaaa gagcagcgcc gccccgagga agaaactgag aaagcgcccc gcggccgtga
    12061 ccgccacgca gacccgcttg cgccgcttca gacgctgcat cagcgccaca cccagcggct
    12121 ggaacaccgc cgccacctgc cccagcgcgc tcaggatgct gaactgcagg gggctggcgc
    12181 ccaggatgac catgaacttg gtgatgaagt tgctgccgat gttggccagg ttgccgtaaa
    12241 tctgagcgaa cacgccttcg atcaccgaaa tccggtaggt ccggaacacg gtgaagctct
    12301 tctgctgcgc tttgtgttct ttgccgcttc tgtcattcac tgacaaatcc caggctcctt
    12361 ttctctgctg tcggttttca tcccctgcca cagctttttc caaattccgg agcctatttc
    12421 cctcagcccc ggtatctgta aagtattgtt ttataaacgc ggcctgagtt acccactata
    12481 tccagaggaa agacgccgcg ggctatctgg aaaaacctga tgaaaacagc aggcttcgcc
    12541 atttacccct tccctctcac atccctttca cttcccgctg agttcccgcc tgtcaaacgg
    12601 gaagtcaaca ggaagtaagt gagaggataa tcggataggg caaaggagga gtcaggttgg
    12661 gagtttccgg gaaattgagg atgactaccc tgagtccagc ccggcggttg cgattgtgcc
    12721 atttgcccgg cttcacataa accttgacca aaaagttagg ggattttgct tggtttggca
    12781 gtatattatc agtgaaacta aaaaactgat gcctttagga ggttacagtg ttcaataaag
    12841 ctgtcttgct catgatcctg ctggcgctgg cttgcgttgc cggcgcggaa gattggatca
    12901 ccatctacaa tgacgacctt tccctggtgc gcagcaattt cgagctggaa ctgcaggcag
    12961 ggcgccagga ctacaatttc gacaacatca cttcccgcat tgaagctccc tccgtgattg
    13021 tgcattccct cgacggcggt gtgcgcgtgg ccgaacaaaa ctatgaatac gacctcgccg
    13081 gcaaatacca gatcatcgcc aaatacatcg aacgcgaggt ggaactgctc accaaagacg
    13141 attcgcgggt aatcggcacc ctcaagttct acgacggcag cagcttcggc atcctggaaa
    13201 aaggcagcag ccgcctgctt gtggtggctg acagcgaaac ccagatcatc aaactggcgg
    13261 aactgccctc caacttttac accaagccaa ccctgcactg gagcctgatt gcggacaagg
    13321 ccggcaggca tcccctccag atgacctatc tcaccggcgg gctgaactgg aacgtaacct
    13381 acaacagcgt ttgggacgaa aataaccttg ccctgaactc ctgggtgacc atcaccaacc
    13441 agagcggcaa ggcattcgac gacgtaaccc tgaaactgat cgcgggagac gtgaacaggg
    13501 tctatgacgt gtataacggc tacaaacgca cttacgaaga aatgatggcg ccgg
//
LOCUS       DAOOWR010000007        13080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_4777_length_13080_cov_4.543186, whole genome shotgun sequence.
ACCESSION   DAOOWR010000007 DAOOWR010000000
VERSION     DAOOWR010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_13080_cov_4.543186"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="PLB85_00510"
     CDS             complement(<1..2323)
                     /locus_tag="PLB85_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOU71837.1"
                     /translation="MHNEKIHLIRIKRLLERQKQLLIRDKLPLETGFSATGGDFQPLA
                     LNSVWGKPWQTGWFRISGSIPEDWSGKDYRLLFDCDGEACLLLDGVPYQGFTPKVDWY
                     HKAAKNLIPLASICKPGEDFSLLIDASANDLFGAQKEEYRLRECALVTFDEGLYQRLL
                     DISLLLDLAETLPEKTVRRQRLVYGLNKVCDAWNTDREMVQSILGDLLSKPAHASALT
                     AFSVGHAHLDLAWLWPLSETRRKGARTFANALRLLEQYPDYVFGASQAQLYQWMKEDH
                     PALYALIKEQVKQGRWEIQGSTWVEFDTNLISAESIIRQFMYGKRFFESEFGQAPRVL
                     WLPDCFGFSGNLPQFLKGCGVDWFVTQKLSWNETNVFPHHLFVWEGIDGSRVLAHQLP
                     TNDYNFSNNPSSFLETEKRYAQSELCDSFLNLYGIGDGGGGPTRDHIEYGLRQRNLEG
                     VSKFRFSSSADFFDHLSGLDSSQLPVAHGELYLEFHRGTYTTQARMKQDNRNSEKLLA
                     AAEFMAVLAGHTYPEKLRCIWQDTLLLQFHDIIPGSSITPVYEEAQTISAANHKLLND
                     YITATTREITDAGTPCKETSYLVVNPSNQELDEWLAFPKELHGLVPLNEYSTELPSLE
                     TENAFLARIRVPAWGCLPLQFSTNGYLPKSGASPASLTLENRFLKVELTETGGVKSIW
                     DKELEHELLASESNLLLLWEDEPNNWGAWDINHFYRDTTPQAASGATLNQALSFTLEE
                     RFSRVVQDIRIGNSSLRQTVELRSDDRLLRVSHE"
     gene            complement(2335..4629)
                     /locus_tag="PLB85_00515"
     CDS             complement(2335..4629)
                     /locus_tag="PLB85_00515"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU71838.1"
                     /translation="MSIIGKVGRRSARVRILNLSIHLVLILGAITMVYPFLLMVSASL
                     RSNVDSGRLNLLPKYLHNDEALFQKYLESRYNEESSRLADNYAGRWLSFAEARLPEKL
                     SPTLFNDWHEFLSARPQGVYDYYVAEHYGRGVYPLNQRRYRKLLRQENDNDLTAFNRR
                     YNTGAQSWEQIVVEEKEILGRNYVSVRDGYLGRFQAFKKAVGERHRNHVNLDGAFAQM
                     ELAPLCGGDLVAFNTDSGLGLNSWQDVVLSESCPPEGHPLRPAWLHYVREALNIHHIS
                     LSASADPAFQAMLKDKYADISLLNSTWHTDYSDFSAVRIPKEIPDSGAMVEDLTWFVE
                     NAAAPEHLRVRNIGNEFRAWLKRKYQSVEVLNSAWDQGVGGWDKLTLPKTAPAENLAL
                     QSDWLEFAQGEGIAWLELLPSAQSDWLALLAERFPGRGGKLDLVQANRYMGSSYSSEE
                     DIYPSPHLPRDNQAAKLWREFVAMRSTPSQLRFSRQKESVQAWQGFLRAKYGTTDSLN
                     QAWHLVATGFDNIPLPVQQIEAQTFLKHKGEIRREFVTRNYAMVLDQMFNDARSLRNT
                     AIYTLLSILLAVTVNPLAAYALSRFKPRLSYQIILLFMLTMAFPAMVMGIPNFLMLKR
                     LNLLNTFWALVLPAAADGYFIFLLKGFFDSLPRELYESASLDGAGEFRLFWQFTLYLS
                     KPILAVIALGAFNAAYRNFLFAFIVCQDQSMWTLMVHIYELMQRASLSVGYAALVIAA
                     IPTLAVFVFFQNIIIKGIVVPMEK"
     gene            complement(4626..6992)
                     /locus_tag="PLB85_00520"
     CDS             complement(4626..6992)
                     /locus_tag="PLB85_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOU71839.1"
                     /translation="MFELPDPRKTDAYSRANLAVVEAFRKKFPHIELRAFSGIQIENM
                     DLDAGPLMAIAGGVAPDILYVNFRQSDTYIQNNFLYPLDEFLASEDPAALSLRVEQPV
                     WQVIRRARKGSKTEYTWMLPYETLVRVLMYRKDVFRRAGLDPQKPPRTWDEFFAYARR
                     LTDPEQGAWGTVLASGPQAAYDWLPFLWGAGGEAVTYDPKKQEWRASFAGEEGEVALD
                     FYLKLVATKWHDNQGRPQTGFALREGDWGYLWQEGKIGMRMDYLSQQNLGGVYDPNLY
                     AFAPAPAGPSGRGGSEINCRMMGIFSGAGISNNSGVGDRDPKAVRQAAWEYIRFYDSE
                     EARQIRMKVMVDSGYGRMQNPVFLKRYGYEEYLRYAPAGWLETFETAMREGRPEPFGD
                     NCQKVYEYMTYPLEELVAMGLKDRLPKDPGQRRKLIASVLRQGEARTNQEMIGHIPPE
                     TKAFRERLAMLIAILILLAFCFTLWRVWKLLSPEAHSSAKLRFNHRVWVVMLLAPAVI
                     TILVWKYLPMVTGSLMAFQDYHIVGASPWIGFGNFATVLFDPAWWASVGKTLYYMALL
                     LGLGFLPPLGLAILLQEVSRGKMLYRVIYYLPAVISGVIVIYLWKLLYDPSDAGILNQ
                     MLLSLGLPKSMWIKDESLAMLCVVLPTVWAGVGPGSLIYLAALKNIPHELYEAADIDG
                     AGFRAKLRHIVLPSLKGLIIIQFISAFIVAAQSSDFILVMTFGGPNEATRVADLMIFE
                     KAYLYLRFGLATAMAWILSLMLMGFTVWQIKYLSRMEFRAVGDDREAA"
     gene            complement(7447..7522)
                     /locus_tag="PLB85_00525"
     tRNA            complement(7447..7522)
                     /locus_tag="PLB85_00525"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7487..7489),aa:Ala,seq:cgc)
     gene            complement(7596..10244)
                     /locus_tag="PLB85_00530"
     CDS             complement(7596..10244)
                     /locus_tag="PLB85_00530"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOU71840.1"
                     /translation="MEISKTYEATQIERKWYRFWEESGYFKPGGDASKPPFTILIPPP
                     NVTGILHMGHVLNNTLQDVVVRYHRMLGEPTLWLPGVDHAGIATQNMVEKDLAKSGQT
                     RHDVGREKMLELIWAWKQDKGDRIIDQLKLLGSSCDWDRLRFTMDEMLSRAVKEVFVS
                     LYEQGLIYKGKYIINWCPRCVTALANDEVEHADETGKLWYIRYPFADGSGHLVVATTR
                     PETMLGDTAVAVNPKDERFQSLIGREVKLPLTGRTIPVIPDEYVEMEFGTGCVKVTPA
                     HDPNDFEIGQRHDLPQLLVMDEHGVMNASAGKDFEGLDRFACREKVVQMLDEQGLLEK
                     IENHEHAVGHCYRCDTAIEPYLSDQWFVKMKPLSERAIEVVEKGEVRFQPERWTKVYM
                     HWMNNIRDWCISRQIWWGHRIPAFYCLECGAMVVAKETPSACPDCGHTAFRQDEDVLD
                     TWFSSWLWPFSTLGWPDETADLKRFLPTQVLITAPEIIYLWVARMIMSTLHFRDVIPF
                     DTVLLHGTVRDELGRKMSKSLGNSPDPIDIIDKVGADALRFSMVFGTPKGADVIYSDA
                     ILETGRNFANKIWNAYRFIMMNVGEGEKLPDRKDLKLELADRWIYSRLNETAREAANH
                     YQNLRLNDAGQCVFQFIWDEFCSWYIELSKDRLNSEDPAARGTALYILLDVMQSAMRL
                     LHPIMPFITEEIWQSVKTVFPQPEEALIVAAFPVCDDTLVDPAIADDMAFMQEAISAV
                     RNLRKQINLSPGARINLAIRVAAEQQKDLFARYSAYFGKLAKVDELDVATDLAKPPAS
                     IAAVVRNIEIFLPLKGLIDFDAERQRLGKQIEKMQKELAGVNAKLQNQNFIANAKAEV
                     VENERQRFAELKTKLDLTKELRADLR"
     gene            complement(10477..11922)
                     /locus_tag="PLB85_00535"
     CDS             complement(10477..11922)
                     /locus_tag="PLB85_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU71841.1"
                     /translation="MNIKLSFSKQLISAMMLVLMAGFLAAQTPLFTFEVDDLEGTNRN
                     TVWLFDFDSPSFATHDGNWYSFGNTPLYNAYPFLHYYAEDLVRMTQPGNSEGQTVISC
                     VQENIPFTDDMTQIGITFTPFDRTSLTQVNLVNPANPWQTHGESGDIRTYANSSGDIT
                     LGGVKKLHIINMQYEITTPYPSGQEIHDYVEHLKGAPYFYPIPFTWNTGDNIGPGTFY
                     QQTGLGTGLVDVNHPDTDPDWAALFASSYVVNIRMLEIVSRTTLNSTYHDFLMEISAD
                     TEPNVPVVMSSFTASVNAENQPVLNWITASENDLMGFRVLGSTASAMNEAINLTPVLI
                     PAQNCNCGASYSFTAWEFNTPGTYWFWLESMDMVGTSDFFGPVSVTVSDTQTPSLPDR
                     SSLGSVYPNPFQSGTAVSIPVEVKAGDSGELSIYNLSGQHVASFPLQQGSHTISWNGL
                     DTSGKSCASGIYFYRLSAGNHQESKKLIILK"
     gene            12392..>13080
                     /locus_tag="PLB85_00540"
     CDS             12392..>13080
                     /locus_tag="PLB85_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOU71842.1"
                     /translation="MKYLKASAIEYLLWLYILGCLAWLLFRLGAYSKPMPAWLLLALP
                     VGLALLLGSRKGCLGRVVAAPDEKPRHQPWKLELGVAILISFVVGWVIVEVKPLAFFT
                     QAGNAQNIIKGIFNPNLQELMPMLAALMETIYLALLATVFAIPFAFLLSFFAAKNLMY
                     GSTLGKIAYVVIRTVSTVFRSIEAIVWAIIFCVWVGIGPFAGMLALWIHSIAALVKLY
                     SEQIENIDPGPV"
BASE COUNT         2720 a         3546 c         3903 g         2911 t
ORIGIN      
        1 tttcgtggct gacacgcagc aggcggtcat cggagcgcaa ttcaacggtc tggcgcaggc
       61 tggaattgcc gatgcggata tcctgcacca cgcggctgaa cctttcttcc agcgtgaaac
      121 tgagggcttg gttcaaggtg gcgccgctgg ctgcctgggg tgtggtatca cggtaaaaat
      181 ggttgatgtc ccaagctccc cagttgttgg gttcgtcttc ccagagcagg agcaggttgg
      241 attccgaggc cagcagttcg tgctccagtt ctttatccca gatggatttt actccgccgg
      301 tttcggtcaa ttccactttg agaaagcggt tttccaaggt cagggaggct ggactggccc
      361 cggatttggg cagatagccg ttggtggaga attgcaaagg caggcagccc caggccggca
      421 cccggatcct ggccaagaag gcgttttccg tttccagaga aggcagttcg gtgctgtatt
      481 cattcagtgg cacgaggcca tggagttctt ttgggaaggc gagccattca tccagttcct
      541 ggttggaggg gttgaccacc agataactgg tttctttgca aggcgttccg gcatccgtga
      601 tctcacgggt agttgcggtt atgtagtcat tcagcagctt gtggttggcg gcgctgatgg
      661 tctgggcttc ctcataaacc ggcgtgatcg aagaaccggg gatgatgtca tgaaactgca
      721 gcagcagcgt gtcttgccag atacaccgca gcttttccgg ataggtatgt ccggcaagca
      781 cagccatgaa ctcggccgca gccagcagct tctcgctgtt gcggttatcc tgtttcatgc
      841 gggcctgggt ggtgtaggtg ccgcgatgaa attccaaata aagctcgccg tgggctaccg
      901 gaagctggct ggagtctagc ccggagagat gatcgaagaa atccgccgaa ctggaaaaac
      961 ggaatttgct cactccttcg aggtttcgct ggcgcagacc gtattcaata tggtcgcggg
     1021 tgggcccgcc gccaccatcg ccgatgccgt aaaggttcaa aaaggagtcg cagagttcgc
     1081 tctgggcata gcgtttttcc gtttccaaaa aggaggaggg gttgttagaa aagttgtaat
     1141 cgttggtggg aagttgatgt gccagcaccc tgctgccgtc gatcccctcc caaacgaaga
     1201 ggtggtgggg aaagacgttg gtctcgttcc agctaagctt ctgggtgacg aaccagtcca
     1261 cgccgcagcc cttcagaaac tggggcagat tgccagaaaa gccgaagcag tcgggcagcc
     1321 acaacacccg gggagcctgg ccgaactcac tttcgaagaa gcgcttgcca tacatgaact
     1381 ggcggataat cgattctgcg gaaatgaggt tggtgtcaaa ttccacccag gttgagccct
     1441 ggatctccca acggccttgt ttcacctgtt ctttaatcaa cgcgtagagg gcagggtggt
     1501 cttccttcat ccactggtaa agctgggcct gggaagctcc gaaaacgtag tccggatact
     1561 gctccagcaa tcgcagcgcg ttggcgaagg tgcgcgcgcc tttgcgtcgg gtttcagaaa
     1621 gcggccaaag ccaggccagg tcgagatgcg cgtgcccaac actgaaggcg gtgagcgcgc
     1681 tggcatgagc cggtttggac agaagatcgc caagaattga ctgcaccatt tcccgatcgg
     1741 tgttccaggc atcgcagacc ttgttcaggc catagaccag gcgctggcgg cgaaccgttt
     1801 tttccggcag ggtctccgcg aggtccagca gcagagaaat gtccagcagg cgttgataaa
     1861 gcccttcgtc aaaggtaacg agggcgcatt cgcgcaggcg gtattcctct ttctgcgctc
     1921 cgaagaggtc gttagccgaa gcgtcgatga gcaggctgaa atcctctcca ggcttgcaga
     1981 tggatgcaag gggaatcaga ttcttggcgg ccttgtgata ccaatccacc ttgggtgtga
     2041 aaccctggta gggaacgcca tccagcagca ggcaggcttc gccgtcgcaa tcgaagagca
     2101 gcctgtaatc ctttcccgac cagtcttccg ggatgctgcc gctgatccgg aaccagcccg
     2161 tctgccaggg tttaccccag acagagttga gcgccagggg ctgaaaatcc ccgccggtgg
     2221 cggagaatcc agtctccaga ggcagcttgt ccctgatcag aagctgtttt tgccgctcca
     2281 gcaggcgttt gatccggatg aggtggatct tttcgttgtg catcggaaac ccctttattt
     2341 ttccatcggg accactatcc ctttgatgat gatgttttgg aagaaaacga agaccgccag
     2401 ggtggggatg gcggcgatca ccagggcagc gtagcccact gagaggctgg cgcgctgcat
     2461 cagctcgtag atgtgcacca tcagggtcca catgctctgg tcctggcaaa cgatgaaggc
     2521 gaagagaaag ttgcggtagg cggcgttgaa ggccccgagg gcgatcacgg ccagaatggg
     2581 tttggaaagg tagagcgtga actgccagaa gaggcggaat tccccggctc cgtcgaggct
     2641 cgcgctttca tagagctcac ggggcaggct gtcgaaaaag cctttgagca gaaagatgaa
     2701 ataaccgtcc gcggcggcag gcagcaccag cgcccagaag gtattgagca ggttcaagcg
     2761 cttgagcatt aggaagtttg gaatgcccat caccatcgcc ggaaaggcca tggtaagcat
     2821 gaagagcagg atgatctgat agctgagccg gggcttgaag cggctgaggg cgtaagctgc
     2881 gagggggttg acagttacag ccagcaaaat ggacagcagg gtgtagatgg ccgtgttgcg
     2941 cagagaacgg gcgtcgttga acatctggtc cagcaccatg gcgtaattac gggtcacgaa
     3001 ctcccgtctg atctcgcctt tgtgcttcag gaaggtctgc gcctcgatct gctggacggg
     3061 cagcgggata ttatcaaagc cggttgcgac cagatgccag gcttgattca agctgtccgt
     3121 cgtgccatat tttgcccgca aaaacccctg ccaggcctga accgattcct tttgccttga
     3181 aaacctcaac tggctgggag tggagcgcat agccacaaac tcccgccaga gttttgctgc
     3241 ctgattgtcc cggggcaagt ggggactggg atagatgtct tcctcggagc tgtaactgct
     3301 acccatgtat cggtttgctt gtaccaaatc cagcttgccg ccccgaccgg gaaagcgttc
     3361 cgcgagcagg gccaaccagt cgctttgagc gctgggcagc aattccagcc aggctattcc
     3421 ttcgccctgg gcgaattcca gccagtcgct ttggagggcg aggttttccg ccggggccgt
     3481 tttgggcagg gttagcttgt cccagccgcc aacaccctga tcccaagctg agttcagtac
     3541 ttccacactt tgatacttcc gcttcagcca ggcacggaat tcgttgccaa tgttgcgtac
     3601 cctcaaatgt tccggcgcgg cggcattttc cacgaaccag gtgaggtcct caaccatggc
     3661 gccgctgtcc gggatttctt ttgggatccg cactgcgctg aaatcagaat agtctgtatg
     3721 ccaggtggaa ttgagcaggg aaatatcggc gtatttatcc ttcagcatag cctggaaggc
     3781 ggggtcggcg gaggcggaga ggctgatatg gtgtatgttc agagcttcgc gcacgtagtg
     3841 cagccaagcc ggccgcaagg gatggccctc cggagggcag ctttcgctca aaaccacatc
     3901 ctgccaggag ttcagaccta aacctgaatc agtattaaag gctacaaggt ctcctccgca
     3961 gaggggggcc agttccatct gcgcgaaggc gccatcgaga ttgacatggt tgcggtgtcg
     4021 ctcgcccacg gctttcttaa atgcctggaa acggccaaga tagccgtctc tcacgctcac
     4081 gtagttgcgc cccaggattt ccttttcctc caccacgatc tgttcccagc tttgggctcc
     4141 ggtgttgtag cgtcggttga aagcggtgag gtcgttatcg ttttcctgcc gcagcagttt
     4201 gcggtagcgg cgctggttca ggggataaac accgcgtccg taatgttcgg cgacatagta
     4261 atcgtaaacc ccttgaggcc gggcggacag aaactcatgc caatcattga acaaagtggg
     4321 agatagcttc tccggcaggc gcgcctcggc aaaggagagc cagcgtcctg catagttatc
     4381 cgcaaggcgg ctgctttctt cattgtagcg ggattccagg tatttctgga acagagcttc
     4441 gtcgttgtgc agatatttgg gcagcaggtt cagtctgcca ctgtcaacgt tgctacgcag
     4501 cgaggccgag accatcagca ggaaaggata gaccatggtg atggcgccca ggatcagcac
     4561 cagatggatg ctgaggttta gaattctcac cctagcggag cgtcggccca ccttgccgat
     4621 gatgctcacg cggcctcccg gtcgtcgccc acggcgcgga actccatgcg ggacagatat
     4681 ttgatctgcc agacggtgaa gcccatcagc atcaggctga ggatccaggc catcgccgtt
     4741 gccaggccga aacgaaggta aagataggcc ttttcaaaaa tcatcagatc cgccacccgg
     4801 gtggcttcgt tcgggcctcc aaaggtcatc accagaatga aatcgctgct ctgtgccgcc
     4861 acgatgaaag cggaaatgaa ctggatgatg atcagtcctt tcaggctggg cagcacgatg
     4921 tggcggagct tggccctgaa gccggcgccg tcgatgtccg cggcctcata gagctcgtgg
     4981 ggtatgtttt ttagcgccgc cagatagatc agagaacccg gtcccacgcc ggcccagacg
     5041 gttggcagca ctacgcagag catggccagc gattcatcct ttatccacat gcttttgggc
     5101 agtcccaggg aaagcagcat ctggttcagg atgccggcgt cggaggggtc gtacagcaac
     5161 ttccagagat agataacgat caccccgctg atcaccgcgg gcaggtagta gatcacccgg
     5221 tagagcattt tcccccgcga aacctcctgc agcaggatgg ccagccccag cggtggcaaa
     5281 aagcccaagc caagcaataa tgccatgtaa tacaacgtct taccaacaga cgcccaccaa
     5341 gccggatcga acagcaccgt ggcgaaattg ccgaaaccga tccaggggct ggcccccacg
     5401 atatggtaat cctgaaaggc catcagcgaa ccggtgacca tgggcaggta tttccagacc
     5461 aggatggtga taactgccgg agcaagcagc ataacaaccc agacgcggtg gttaaacctc
     5521 agtttggcag aactatgcgc ttccgggctg agcagcttcc aaaccctcca gagggtgaag
     5581 caaaaagcta gcaaaatcaa gatggcgatc agcatcgcca ggcgttcccg gaaagccttg
     5641 gtctctggcg ggatgtggcc tatcatttcc tggtttgtgc gcgcctcgcc ttggcgcagc
     5701 acggacgcga tcaatttcct cctctgtccg ggatcctttg gcagcctgtc tttcaggccc
     5761 atcgccacca gttcctccag cggataggtc atgtattcat acactttctg gcagttgtcg
     5821 ccaaaaggtt ccggccggcc ttcgcgcatg gcggtctcga aggtttccag ccagcccgcg
     5881 ggggcgtagc gcagatactc ctcgtagccg taacgtttca ggaacacagg gttttgcatg
     5941 cgtccgtaac cgctgtccac catcaccttc atgcggatct ggcgggcctc ctctgaatcg
     6001 taaaagcgga tgtattccca ggcggcctgg cgcacagctt tgggatcgcg gtcacccacc
     6061 ccggaattgt tgctgatccc ggccccggaa aagatgccca tcatgcggca gttgatttcg
     6121 ctaccgccgc gtccggaagg cccagccggg gcaggggcga aggcatagag gttcggatcg
     6181 taaaccccgc caaggttttg ctgggaaaga tagtccatcc gcatgccgat cttgccttcc
     6241 tgccagagat agccccagtc cccttcccgc agggcgaaac cggtctgcgg gcgtccctgg
     6301 ttgtcgtgcc atttggtggc caccagcttc agataaaaat ccagcgccac ttcgccttcc
     6361 tctccggcga aggaagcgcg ccattcctgc tttttggggt cgtaggtgac ggcttcgccc
     6421 ccggcgcccc agagaaaagg cagccagtcg taagcagctt gcggaccgga agccaaaacc
     6481 gtgccccagg cgccttgctc aggatcggtg agccggcgcg cgtaagcgaa aaattcgtcc
     6541 caggtccgcg gcggtttttg cgggtcgagc ccagcccggc gaaacacgtc ctttcggtac
     6601 atcagcaccc gcaccagcgt ttcgtagggc agcatccagg tatattcggt tttgctgccc
     6661 ttgcgggcgc ggcgaatcac ttgccagaca ggctgttcca cccgcagaga cagcgcggcg
     6721 gggtcttcgg aggccaggaa ttcatccagc ggatagagaa aattgttttg gatataggtg
     6781 tcgctctggc ggaaattcac gtagaggata tccggcgcca ctcctccggc gatggccatc
     6841 aggggaccgg cgtcgaggtc catgttctcg atctggatgc cggaaaaggc gcgcaactct
     6901 atgtggggaa acttcttgcg gaacgcctcc accaccgcca gattggcccg cgaataggca
     6961 tcggtcttgc ggggatcggg cagctcgaac accttcaaaa ccacacgatt agcgtttttt
     7021 gcccacgcca gggcaggcgc cgttatcaac agcgccgcaa gcagaagaaa agacgaaaat
     7081 ctgcgcataa tcctgattcg caacctggtt cgcggcgccg ctttctgtca agcacaaaat
     7141 tgccggcatt cccctatttt cagagcaacc tggttttcat ctgccttgct tccgcaagat
     7201 gggatctttc aagcgacttg ttgtggaggg tgtccctgtg cagacggatt ttgcggagga
     7261 gcgaagcgat accgtctggc cgttaaggcc tttcgatgtg ttgcggattc gcctcccgca
     7321 caatggccgc atttgatgcg gggatttggc gtggggcgtg ggaggggtat ggaaacgagc
     7381 gctaagggcc tgaccgaaag caggtttggg aaggtaaagc gggtaattgc aaggcgatag
     7441 agaaagtggt ggagctaagg ggattcgaac ccctggcctg aacattgcga acgttccgct
     7501 ctcccaactg agctatagcc ccactttcta tcgctgtgag cggctttcag ggccgccgtg
     7561 ccggtctctg ctcgaaaccg ggaaaagatg tatcgttacc tcaaatcagc gcgcagctct
     7621 tttgtcaagt ccagttttgt cttgagctcg gcaaagcgct ggcgctcgtt ttccaccacc
     7681 tcggctttgg cgttggcgat gaagttctgg ttttggagtt tggcgttcac tccggcgagt
     7741 tccttttgca ttttttcgat ctgtttgccc aggcgctggc gctccgcgtc gaagtcgatg
     7801 agccctttca ggggcaggaa aatctcgatg ttgcgcacca cggcggcgat ggaggcgggc
     7861 ggcttggcca ggtctgtggc cacatcaagt tcgtcaacct tggccagttt gccgaaatag
     7921 gcggaatagc gtgcgaaaag gtctttctgc tgctctgccg cgaccctgat ggcgaggttg
     7981 atcctggctc cgggagagag attgatctgc ttgcggaggt tgcgcacggc ggagatggct
     8041 tcctgcatga aggccatgtc gtcggcaatg gcgggatcga ccaaagtgtc gtcgcagaca
     8101 gggaaagccg ctacgatcag ggcttcctcg ggttgcggaa acacggtttt cacgctctgc
     8161 cagatctctt ccgtgatgaa gggcatgatg ggatgcagca ggcgcatggc agactgcatc
     8221 acgtcgagaa ggatatagag cgcggtgccg cgcgcggcgg ggtcctcgga attgaggcgg
     8281 tctttggaaa gctctatata ccaggaacag aattcgtccc agatgaattg gaacacgcac
     8341 tgcccggcgt cgtttagcct caggttctga tagtggttgg ctgcttcgcg ggcagtttca
     8401 ttgagccggg aatagatcca gcggtcggcc agttccagct tcaaatcctt tctgtcaggc
     8461 agtttttcgc cctcgcccac gttcatcatg atgaagcgat aggcgttcca gattttgttg
     8521 gcgaagttgc gcccggtctc cagaatggca tcggaataaa tgacatccgc gccttttggg
     8581 gtgccgaaga ccatggaaaa gcggagggcg tcggctccga ccttgtcgat gatgtcgatg
     8641 ggatcgggcg aatttcccaa ggatttgctc atcttgcggc caagttcgtc gcgcacggtg
     8701 ccgtgcagga ggacagtatc gaaaggtatc acatccctga aatgcagggt gctcatgatc
     8761 atgcgggcta cccagagata gatgatttcc ggggctgtga tcagcacctg ggtgggcaga
     8821 aagcgtttca ggtcagccgt ttcgtcgggc cagcccagag tggagaaagg ccagagccag
     8881 ctcgaaaacc aagtgtccag aacgtcctca tcctggcgga aagccgtgtg gccgcaatcc
     8941 ggacaggcgg aaggggtttc tttcgcgacc accatggcgc cgcattccag gcagtaaaaa
     9001 gccgggatgc ggtgtcccca ccagatctgg cgggagatgc accagtcgcg gatgttgttc
     9061 atccagtgca tgtaaacctt ggtccagcgt tcaggctgga agcgcacttc gcccttttcc
     9121 accacctcga tggcgcgttc ggacaggggc ttcatcttca cgaaccactg gtcggaaaga
     9181 taaggctcga tcgctgtgtc gcagcgataa cagtgcccta cggcgtgttc gtggttctcg
     9241 atcttttcca gcaggccctg ctcgtcgagc atctgcacca ctttctcgcg gcaggcaaag
     9301 cggtccagcc cctcaaaatc ctttcccgca gaggcgttca tcacgccgtg ctcatccatc
     9361 accagaagct ggggcaggtc gtggcgttgg ccaatctcaa agtcattggg atcgtgggca
     9421 ggagtaactt tcacgcagcc ggtgccgaat tccatctcca catattcatc cggaataacg
     9481 ggaatcgtcc tgccggtaag gggtagctta acttctctgc cgatcaggct ttggaagcgt
     9541 tcgtccttgg gattcactgc cacggcggta tcgcccagca tggtttccgg acgcgtggtg
     9601 gcaaccacca gatggccgct gccatcagca aaggggtagc gaatgtacca gagttttccg
     9661 gtctcgtcgg cgtgttccac ctcgtcgttg gccaaagcag tcacgcagcg cgggcaccag
     9721 ttgatgatgt attttccctt gtagatcagg ccttgttcgt agaggcttac gaagacttct
     9781 ttcacggcgc gggagagcat ttcatccatg gtgaagcgca gccggtccca atcgcaggag
     9841 gagcccagaa gcttgagctg gtcaatgatg cggtcgccct tgtcctgttt ccaggcccag
     9901 atgagttcca gcattttttc gcggccgacg tcgtggcgcg tctgtcctga tttggccaaa
     9961 tccttttcca ccatgttctg ggtggcgatg ccggcgtgat cgacgcccgg aagccaaagc
    10021 gtgggttcgc ccaacattct gtggtagcgc accaccacat cctgcagcgt gttgttgagc
    10081 acgtgaccca tgtgcaggat gcccgtcacg ttcggcggcg ggatgaggat ggtgaaaggc
    10141 ggtttggatg cgtctccgcc cggcttgaaa tagccgcttt cttcccagaa ccgataccat
    10201 ttccgctcga tttgagttgc ttcgtaagtc ttgctgatct ccatttatgt caccttgata
    10261 ttatcccaaa aagcgggatc gcttgatttt atatgtgaaa ataacctaaa aaagtcacgg
    10321 cgatgtgttg tcaagcaaat aaagccccgc gaggcccgga agcctgctcc ttctcccatt
    10381 tgaagccccg ctccatcctg gcggttgctt ttcctcagta aaagaaaaac ccggccgctg
    10441 gggccgggtt ccttctctct gaatggaaga aggtgtttat ttaaggatga tgagtttctt
    10501 gctttcctgg tggttgccgg cgctgaggcg gtagaaatag ataccgctgg cgcaggattt
    10561 gccgctggtg tccaggccgt tccaggagat ggtgtggctg ccctgctgca gagggaagga
    10621 agccacgtgc tggccggaga ggttgtagat gctgagttcg ccgctgtcgc cggctttcac
    10681 ctcgacgggg atgctcaccg cggtgccgct ctggaaggga ttgggataga cgctgcccag
    10741 gctgctgcgg tccggaagtg acggggtctg ggtatcgcta acggtcacgg agacagggcc
    10801 gaagaagtcg cttgtgccca ccatgtccat gctttccagc cagaaccagt aggtgccggg
    10861 ggtgttgaat tcccaggcgg tgaagctgta ggaagctccg cagttgcagt tttgggccgg
    10921 gatcagcacc ggggtgaggt tgatggcctc attcatggcc gaagctgtag agcccagaac
    10981 ccggaatccc atcaggtcgt tttcggaggc ggtgatccag ttcaggacag gttggttctc
    11041 ggcgtttacc gaggcggtga agctgctcat cacaactggc acgttgggtt cggtatcggc
    11101 ggaaatctcc atcaggaaat cgtgataggt ggaattcagg gtggtgcggg acacgatttc
    11161 cagcatcctg atattcacca catagctgga ggcgaagagc gcagcccagt ctggatcggt
    11221 gtcgggatgg ttcacatcca ccaatccggt gccgaggccg gtttgctgat aaaaagttcc
    11281 ggggccgatg ttgtcgccag tgttccaggt aaacggtatt gggtagaaat aaggggcacc
    11341 tttcaggtgt tccacatagt catggatctc ctggccggag gggtaaggtg tggttatctc
    11401 atactgcatg ttgatgatat gcagtttctt cactccgccc agagtgatat cgccgctgga
    11461 attggcatag gtgcggatgt ctccggattc tccatgtgtc tgccagggat ttgcaggatt
    11521 gaccaggttt acctgggtca ggctggtcct gtcgaaaggc gtaaaggtga tgccgatctg
    11581 ggtcatgtca tccgtgaagg gaatgttttc ctgaacgcag cttatcacgg tttgcccttc
    11641 cgaattaccg ggctgggtca tgcgcaccag gtcttcggcg taatagtgca ggaagggata
    11701 tgcgttataa agcggtgtgt ttccaaagct gtaccagttc ccgtcatgcg tggcgaagga
    11761 agggctgtcg aaatcgaaga gccagaccgt attgcggttg gtgccttcga ggtcgtcaac
    11821 ctcaaaagtg aacaaagggg tttgcgcggc cagaaatccg gccatcagca ccagcatcat
    11881 ggcgcttata agttgcttgc tgaacgaaag cttgatattc atttcagttc tcctagaatc
    11941 aaagttaact tgaaaaacca tcattttaac ccctgcctat cttggcaagg attttttcat
    12001 tatgtcattc cgagtctctc aatcagaatt gacaaaccac taaaaaggtg atgaacagcc
    12061 ccagccattt cgacttcctt catcccaggg ctggaaactc cacagaaaag cgattcgatg
    12121 ctgattggcc gttaaggccc tgtattgaac ccctgacgcg cctcccgtat gatgcccgca
    12181 ctaaatacga ggatttggcg ggaggaaatg gagggagata agctcgggcg ctaaggacgc
    12241 ggctggtagg atacgatggt gggtagcccg cagggcggca gaacgatagt gagggtttga
    12301 aagtgagcct ttcatttcgc cgaaccggac caggatgggc cggagcaaaa actgcttgac
    12361 ttttccgccc cctttttcaa cttaccactc catgaagtat ctcaaagcaa gcgcgatcga
    12421 atatctgctc tggctctaca tcctgggctg cctggcctgg ctgcttttcc ggctgggggc
    12481 ctattccaag ccaatgccgg cctggctgtt gctggctttg ccagtgggac tggccctgct
    12541 gcttggctcc agaaaaggat gcctaggccg ggtggtggct gctccggatg aaaagccccg
    12601 ccaccagccc tggaagctgg aactgggtgt ggccatcctg atcagctttg tggttggctg
    12661 ggtgatcgtg gaggtgaagc cgctggcctt tttcacccag gcgggaaacg cgcagaacat
    12721 catcaaaggc atcttcaatc ccaatctgca ggaactgatg cccatgcttg ccgccctcat
    12781 ggaaaccatc tacctggctt tgctggccac ggttttcgcc attcccttcg cctttctgct
    12841 cagctttttc gccgccaaga acctgatgta cggctccacc ttgggaaaaa tcgcctacgt
    12901 cgtgattcgc accgtctcca ccgtcttccg ttccatcgag gccatcgtct gggccatcat
    12961 cttctgtgtc tgggtcggaa tcggcccctt tgccggaatg ctggcgctgt ggatccattc
    13021 catcgccgcg ctggtgaagc tctattccga gcagatcgaa aacatcgatc ccggccccgt
//
LOCUS       DAOOWR010000008        12864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_4858_length_12864_cov_3.994067, whole genome shotgun sequence.
ACCESSION   DAOOWR010000008 DAOOWR010000000
VERSION     DAOOWR010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4858_length_12864_cov_3.994067"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLB85_00545"
     CDS             complement(<1..949)
                     /locus_tag="PLB85_00545"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOU71843.1"
                     /translation="MKRNDLLLTALSALLLALSRLPLHLGWLVFFAWLPLLRVFERGQ
                     ASSRDLLRMGFIMSVVYCGIVFYWLFYVHPGALPGVMLVYIFAYYLVFYGINRIFLTL
                     PRWRWVGFLSILITFEYIQNFGETRFPWFNQAYSLAEYGVLIQAADLIGVVGLSVLII
                     CVNLLLFQLCPCLPKRLRNRSQSVVELPEAFGKKQIGVLAALLLIFAVWFGYGWHCQT
                     RLKLERHDACIHVMQPSIPQVLKWEPEQYEASLAIFRDLTQKAVADSARLVIWPEGAM
                     IGDLDRVSRARRDLRALIDSTQVDVFTGFQHYVYDPSHPV"
     gene            complement(936..1757)
                     /locus_tag="PLB85_00550"
     CDS             complement(936..1757)
                     /locus_tag="PLB85_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71844.1"
                     /translation="MLRKSTILSVFILFAILLAGCAENRTARPELESSRERVAEFPVA
                     RVYTKAVSDAMGKTVYAYVRSSQEIDIFVDGQRVNSLGGLGFERGNFQGLSDIGVDAD
                     GGLLALDSARKLLRKFSPEGMFISEFSLKSLSQPELFCAAPDGTLFVYDAAPSEIICY
                     SPLDGREFNRFGRFELDQPQNLDCNKDYLWAQNRDRESTQVFSLLGQLEYSAKVFRAL
                     DRVGNMISYGRVEGVSNVLQNPLSLTVQGDLVTVLWENEIWMDRLVYGRRGGETQ"
     gene            complement(1765..3483)
                     /gene="recJ"
                     /locus_tag="PLB85_00555"
     CDS             complement(1765..3483)
                     /gene="recJ"
                     /locus_tag="PLB85_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU71845.1"
                     /translation="MKKEWLFQDEFSPEQQQQIDALTEQLKCPQLVAELLFRKGLNTS
                     QQIEDFFNPQLSHQHDPFLFPDMDKAVDRILSAIDRGEKILIYGDYDVDGTTATTLLY
                     LGLQRLGAIIDFYIPHRMIDGYGLSLGSLDQIKESGTRLIISVDCGVNALEEIEAINS
                     HGMDIIITDHHNPKEDLPNAFAIINPKLPGCPYPFAHLAGVGVAYKLLMAIYRRRGID
                     NEENTLKYMDLVAVGTIADIVPLTGENRIFAHIGLQHLVDKKNLGLNALVQISGLNTK
                     NMDASDIVFGIAPRINAAGRMGSASTAVELLISRKKERSLELAEIIERQNSLRQQQDQ
                     KTFHEACDIIEKKYKDISTAPCIVVSSDDWHPGVIGIVASKLVEKYSRPVVMISFKDG
                     FGSGSGRSMADFDLFGALRAIGHNLHSFGGHKYAVGFTILQEYIDRFENELARYIAEH
                     HQISQVNLPLEIDNQIELYDISNFLLDWIERFAPFGPENQRPLLVTRDVMVASYPYTV
                     GRNHLKLKVVKDGVVLDLIGYNLGDYLSLLKKNARLNIAYTLEYNRFGTKTSIQGKLR
                     DLHLLE"
     gene            3920..4228
                     /locus_tag="PLB85_00560"
     CDS             3920..4228
                     /locus_tag="PLB85_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU71846.1"
                     /translation="MNIDLSFNGDRALMVVEGFLNSDNASVLQAKLDEVLQSGARYLD
                     IDLFECKNISSTGIGKLFLFYKDFIGKGGEIEVVRSSDSIYELFSMLKLNQLFNINLE
                     "
     gene            4231..5151
                     /locus_tag="PLB85_00565"
     CDS             4231..5151
                     /locus_tag="PLB85_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU71847.1"
                     /translation="MQTHSILIVDDNPELAKSLGKLLAEHNYRVETASRCSDALRKLR
                     RNLYDIVVCDIDMPGMNGLDLLEKIRQDGRAQEVILMTGFMEQEHFVRAIRLGASDFI
                     NKPVEIQHLLKSIEIITARLIDRRNKVNMFNSFEQAEFSCVIDPQMYSAAGVTKILNP
                     ILQKNLELPHDTLSDLLTCIDEMLQNAFIHGILELTDEERLSEHDQLCELIRRKLLQP
                     EIVGRRMRFSMWMEKEKEAIAICVEDDGPGFDYESWLKRLRADDNLSMEAHGRGLAML
                     YFLSDHLEFGNGGRKVMMLRKLQPRRNAQA"
     gene            5148..5975
                     /gene="prmC"
                     /locus_tag="PLB85_00570"
     CDS             5148..5975
                     /gene="prmC"
                     /locus_tag="PLB85_00570"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU71848.1"
                     /translation="MTLASLLESLRPGLLPSSGLNFLLAGLLDCSVPALKLQHNRKLA
                     PPQQASIRDALLRLEKGEPPQYILGKTWFYGLELKVDPRVLIPRPETEGLVKLALERL
                     KPEARVLEIGTGSGAIAIALKKTGPNLSVMATDISSAALELARDNAFRHNCAIDFVEA
                     DLFPPGDFRFDLVVSNPPYISEKEYSELEPMVRDYEPRQALLAAEEGLEYYRYLFSGL
                     PNQLNDGGLALFEHGALQRQSIIALGAEAGFECCLAKDDLAGRNRYLGFRLKRNTEK"
     gene            5992..7245
                     /gene="murA"
                     /locus_tag="PLB85_00575"
     CDS             5992..7245
                     /gene="murA"
                     /locus_tag="PLB85_00575"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU71849.1"
                     /translation="MDRFIIEGNRELSGTIQVSGAKNAILPVMAACLLAPGKSVLRNV
                     PNLIDLKTMAHLLRVLGARVVYENGMMSIDSRDVNFNEAPYELVSKMRASIYVLGPLL
                     ARLGEARVSFPGGCAIGTRPVDLHLQAMEALGAKIEIEHGYIHATADRLTGADIHFAK
                     SSVGATINALMAAVLASGTTRMFNAAMEPEVDSTIELLNKMGAKISGQGSTTLTIEGV
                     DDLFPVEMRMISDRIEAGTFLIAGALSTKPVTVANCQPEHLGILLEKLRAAGCEIDVQ
                     ASNITVVPPQVIKPVDILTLPYPGFPTDLQAQFTVLMALAEGTCFIEDTIFPDRFMHV
                     AELNRLQADIRMERNVAHVKGVKAFSGAEVMATDLRASAALVLAGMVAEGTTTVSRIY
                     HIDRGYDRIEEKLNSIGARITREMA"
     gene            complement(7919..10447)
                     /locus_tag="PLB85_00580"
     CDS             complement(7919..10447)
                     /locus_tag="PLB85_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU71850.1"
                     /translation="MMNAKRFTLLIVFLACAIASLAALPSAFTSKGNPGLYKTLRSKA
                     LGNLAKLDRHQRKEYRRLLKEQDDVLMAYLLAYESDANLQIARPADVLSNYLHVRALL
                     NTHGTSLDPEFYLSYVAKQTVSDERIEAYREGLLRDGLREIMESSTDEIDLYRKVSQW
                     CVGRLKFQPTSGRDQTPLDITQKSLLGRCEEMQILFVAAARTVGLPSRPASTPWWPHQ
                     DNNHAWAEVWLEGAWHYTGDMDAAYWPDQTWFSGLIDKTVLILADGSLPAASDEVLIR
                     GKYDCVINSIRNYAGERTRSLSIQTLDEQGNPLPNTSVGVMVVNWSALRPLAWLKTDA
                     EGKLAFSVGRGAFYLAAEQDGKRALELVPSSDSTLINCDLTLKAGPLADRDDRLVYPS
                     NPFEWKQAPEYWNEGVKREKERWNAIDRSWARVAASQADSLNAEFVKAARGNYAGALE
                     FLRRYPHPCEGFIEDCLAEEWGIMDPKFLWQASADQIEAVYHAYLKYEDDENIGQDMG
                     SLIFPSVRYEELPQPLGFRNGIPSFYPRSFYQQGETRLERLNRAARWLKRNYKIDPDK
                     ALSGMPPLHVVIGQKYLNNAQYKLMAVSLARANGIPADAGFRSKHIGVRFDDGDSGYY
                     DLETCAPEIDPDEQESLVKLTVLVSDESGAPLGSNERAMLILSDIREGDYAWLEGYSG
                     TDEGNGVLSFQVPKGECYLSAIYRISDSLTAFQTKHLDLDDSLTVEIFLKDYPRGWNN
                     GVYYELTDLLTPADTTGFEIFLIGNHDQENSIRLAEKLRSLGRKFLWVGYEPAPQPIA
                     NYVVSQLWAQWMTEDQRNRARTITLTLKNGKWDSYEGLWDHLPE"
     gene            complement(10572..>12864)
                     /locus_tag="PLB85_00585"
     CDS             complement(10572..>12864)
                     /locus_tag="PLB85_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU71851.1"
                     /translation="TLPPVWQQLAKKGVEPADFFVNPQDLNKYYVPSSAKNYDYLIVS
                     TPELYTAIAPLETYRQGQGLITAFADINTILNSSPGATAGEKLRNHLIAQYHSNPFSY
                     LLLVGDYDKVPVMYLTPEPDGSETVASDFFYGDLSSIVDTDSDGRLGEYSSGEGIQDF
                     LCDFTPEVFVGRISNNSPVAVAQIAARTVAYEQTNAPWKRKALLPAAYLNYGGEPETI
                     YLQTDGATFMELARQTILSDYDCTTMYERYGFLPSYPCDIDLDYDLLKNELNTNSYGL
                     LNWSAHGSSGSSSRKVWMNDDNNNGLPDSWEMNWMGMVDRQSFNNLANPDGMVVFAAS
                     CYNGAIDSYQPCLAEYALQKKAVAVTAATRTGWYKIGWANPGWGGLSSYNYHWLENIA
                     SNGMTVGAAQAFANLTHCQYYLFGNPVDAGGIIWPELQNVYTYLLYGDPAIGYIGQQN
                     YSNGEILVYEPSHQDGLPIVEALDLAHFNVVYTDKLIPDHDYINQFEAVFCLFGWGDT
                     AYVLNPDSLDYALLNSYLNSGGKLYLEGDVSWDSQDPFWGKFATQASLENFAYIEDLA
                     TTQAGHTYTWGYDAQADPHTQILMPTNSSSEVFISTQNLQHPDHPVAILNVTDNYATV
                     ASSFALADVDDTPPYSSDFRFLLGTILDRLGVIDYLPTDANDPQVPAPELSARPFPNP
                     FTAGMTLQVELPKASAARLEIYNIRGQKVYSRSYLTLTAGAHELLWDGCDTRGEMVSS
                     GLYFWRFSAGENSLSGKMLKLAE"
BASE COUNT         2937 a         3487 c         3584 g         2856 t
ORIGIN      
        1 ccacaggatg tgaagggtcg taaacatagt gctggaagcc ggtgaacaca tccacctggg
       61 tgctgtcgat caaggcacga aggtctctcc gagccctgga aacgcggtcc agatcgccga
      121 tcatcgcccc ttccggccag atcaccagcc gagccgaatc ggcgacggct ttttgggtca
      181 ggtcgcgaaa aatggccagg gaagcttcat attgctctgg ctcccatttc agcacctgcg
      241 ggatggaggg ttgcatcaca tgtatgcagg catcatgtcg ttccagcttc agccgggtct
      301 ggcagtgcca gccatagccg aaccaaacgg caaagatcag cagcaaggcg gccaaaaccc
      361 cgatctgttt ctttccgaat gcctcaggca gctccacaac cgattgagag cggttccgaa
      421 gcctttttgg caggcagggg cagagttgga acagcaacaa gttaacacag ataatgagca
      481 cagagaggcc caccactccg attaagtcag ccgcctggat cagaacgcca tattccgcca
      541 gggaataagc ctgattgaac cagggaaagc gggtttcgcc gaaattctgg atgtactcga
      601 aggtgatgag gatgctcaga aaacccaccc agcgccagcg gggtagggtc aggaagattc
      661 ggttgatgcc gtaaaacaca agatagtagg caaatatata aaccagcatc actcccggca
      721 aggctcctgg atgcacgtaa aacagccagt aaaaaactat cccgcaatag accaccgaca
      781 tgatgaaacc catgcgcagc aggtctcggg aactggcctg gcctcgttca aaaaccctca
      841 gcaacggcag ccaggcgaaa aaaaccagcc agcccagatg cagtggcagg cgtgacagcg
      901 ccagaagcag cgcgcttaag gcagtcagca gcaagtcatt gcgtttcacc gcctctcctt
      961 ccgtaaacca gcctgtccat ccagatctcg ttctcccaca acaccgtaac cagatcgccc
     1021 tgcacggtga gggacagcgg attctggaga acattgctaa caccctcaac ccggccatag
     1081 ctaatcatat tgcccaccct gtccagggct cggaacacct tggccgaata ttcaagctgc
     1141 cccagcagtg aaaagacctg agtgctttcc cggtcccggt tttgcgccca tagataatcc
     1201 ttgttgcagt ccaggttctg aggttggtcc agttcgaaac gcccgaagcg gttaaattcc
     1261 ctgccatcga gcggtgagta acagattatc tcagagggag cagcgtcgta aacgaaaagc
     1321 gtgccatccg gagccgcgca gaaaagttca ggctggctga gcgattttaa gctgaattcc
     1381 gatatgaaca ttccctcggg actgaatttg cggagcagtt tccgggctga atccaaagcc
     1441 agcaaaccgc catcagcatc cacgccgata tcgctgagcc cctggaagtt gccgcgctcg
     1501 aagcccagtc cgccaagact gtttacccgc tgcccatcca caaagatgtc gatctcctgg
     1561 ctggaacgca cgtaggcata gacagtttta cccattgcat cactgacggc tttggtatag
     1621 acgcgggcaa caggaaactc cgccaccctt tcccggctgc tctccagttc aggcctggcg
     1681 gtcctgtttt cggcgcaacc cgccagcagt atggcaaaga ggataaaaac ggaaaggatt
     1741 gttgatttgc gtaacatggt attcctattc aagcaggtgg aggtcgcgca gcttgccctg
     1801 gatgctggtc ttggtgccga agcggttgta ttcaagggtg taggcgatgt tcaacctcgc
     1861 gttttttttc agcaggctga ggtagtcacc gaggttgtag ccgatcaggt ccagcaccac
     1921 gccatcttta accaccttta gtttcagatg gttgcgcccc accgtgtatg gatagcttgc
     1981 caccatcacg tctctggtaa ccagcaaggg tctttggttt tccgggccga agggcgcgaa
     2041 gcgctcgatc cagtcaagca ggaagttgct gatgtcataa agctcgatct ggttgtcgat
     2101 ctccagcggc agattcacct ggctgatctg gtgatgttct gcaatgtagc gggcaagctc
     2161 attctcgaag cgatcgatat actcctggag gatggtgaag cccacagcat atttatggcc
     2221 gccgaagctg tgcaggttgt gtccgatggc gcgcagggca ccgaagaggt caaaatccgc
     2281 catgctgcgt ccggaaccac tgccaaaacc atccttgaaa gagatcatta ccaccgggcg
     2341 ggaatacttt tccaccagct tggaggccac gatgccgatc acaccgggat gccagtcgtc
     2401 ggatgagacc acgatgcagg gcgctgtgct gatgtccttg tatttctttt cgatgatgtc
     2461 gcaagcttca tggaaagttt tctggtcctg ctgttgacgc agggagtttt gcctttcgat
     2521 gatttcggcc agctccaggc tgcgttcctt cttgcgggaa atcaacagtt ccactgcagt
     2581 ggaggctgat cccatcctgc ccgcggcatt gattcgcggc gcgatgccga acacaatatc
     2641 cgaggcgtcc atattcttgg tgttcaaacc tgatatctgc accaaggcgt tcagacccag
     2701 atttttcttg tccaccagat gctggagccc gatgtgggcg aaaatgcggt tttcgcctgt
     2761 gaggggaacg atgtccgcta tggtgccaac cgccacaagg tccatgtatt tcaaggtgtt
     2821 ttcttcattg tctatgcccc gccggcggta gatggccatc agcagtttgt aggccacgcc
     2881 aaccccagcc agatgggcaa agggataggg acagcctggc agcttgggat tgatgatggc
     2941 aaaggcgttg ggaagatctt ccttgggatt gtggtgatcg gtgatgatga tatccatccc
     3001 gtgggagttt attgcctcga tctcttccag ggcgttaacg ccgcaatcca cgctgatgat
     3061 cagcctggtg ccgctttcct tgatctggtc gaggctgccc agagacaaac cataaccgtc
     3121 gatcatgcgg tgcgggatgt aaaaatcgat gatggcgccc aaacgctgca accccagata
     3181 gagcaaggtg gtggctgtgg tgccgtccac gtcgtaatct ccgtagatga ggatcttctc
     3241 accccggtcg atggcgctca ggatgcggtc gacagccttg tccatgtccg gaaagaggaa
     3301 gggatcgtgc tggtgactca gctgggggtt gaagaaatcc tcgatctgct gggacgtgtt
     3361 cagcccttta cggaaaagga gttcagccac aagctgcgga cacttcaact gctccgtgag
     3421 agcatcgatc tgttgctgtt gttcagggga aaactcatcc tggaatagcc attccttctt
     3481 catttagcca ccattatagt attctcttgt ttaccagata gttgcgtatt gttatttaaa
     3541 tctcaggatt cggaaatccc attcaccgga acggcaatca aggagttccg gggtacttgg
     3601 agcaactctg gacaggactt caatcttgta aaggaaaaag ttccggttcc gcttccaacg
     3661 gcctttccca agatcatctg ttttcagaaa catggactga gacagattgt ccagttcgtc
     3721 agagacagtc tctgccaagt aatcagaatc tacctgcaat aggttaaccg ctaagacaag
     3781 tctccatatt gagccatgtt gaccgaacca atgcaaatga aaatcaggct gatttgagaa
     3841 aagggctgat aatctgcttg acagatgggg agcaaaaagt aaatatggac tcaaacatga
     3901 agcatacaag gggatggtta tgaacattga tctcagcttc aacggcgaca gggcgctgat
     3961 ggtcgtcgaa ggctttctca acagcgacaa cgcatcagtc ctgcaggcaa agttggacga
     4021 agtgctgcaa tcgggcgcgc gttatctgga tatcgacctg tttgagtgca agaatatcag
     4081 cagcaccggc atcggcaaat tgttcctttt ctacaaagat ttcatcggca agggcgggga
     4141 gattgaagtg gtgcgcagtt cggactccat ctacgaactg ttcagcatgc tcaagctcaa
     4201 ccaacttttc aatattaatc tggaatgact gtgcaaaccc acagcatcct tatagttgac
     4261 gacaatccgg agctcgcgaa gagcctgggc aagctgctgg ccgagcacaa ttatcgggtg
     4321 gaaacagcca gccgctgcag tgacgccctc cggaagctgc gcagaaacct atatgacata
     4381 gtggtttgtg atattgatat gcccggcatg aacggactcg atcttttgga aaagatccgc
     4441 caggatggtc gcgctcagga agtgatcctg atgacaggct ttatggaaca ggaacacttt
     4501 gtccgcgcca tccgccttgg cgcttccgat tttatcaaca aaccggtcga aatccagcac
     4561 ctgctcaaat ccattgagat tattacggct cgcctcattg accggcgcaa caaggtcaac
     4621 atgttcaata gcttcgaaca ggctgagttt tcctgcgtta tcgatcccca aatgtattcc
     4681 gcggcgggcg tcaccaaaat tctcaatccc atcctgcaaa aaaacttaga acttccccac
     4741 gacaccctca gcgacctgct cacctgtatc gatgaaatgc tgcaaaacgc tttcatccac
     4801 ggaatcctgg aactcacaga tgaagagcgg ttgagcgagc atgaccaact gtgcgaactc
     4861 atccgccgca agcttctcca gccggaaata gtcgggcgcc gcatgcgttt ttccatgtgg
     4921 atggagaagg aaaaggaagc catagctatc tgcgtggagg atgatggccc gggctttgac
     4981 tacgagtcct ggctgaaacg attgcgggcg gatgacaacc ttagcatgga agcacatggc
     5041 cggggcctgg caatgctgta ttttctttcc gaccacttgg aatttgggaa cggtggccgc
     5101 aaagtgatga tgctgcgcaa actccagccc cggaggaacg cccaggcatg accctggcat
     5161 ccctgttgga aagtttgcgg cccgggctgc tgccctcctc cggtctgaat tttctgctgg
     5221 ctggattgct cgattgttcc gttcccgctc tcaagctgca acacaacagg aaactcgccc
     5281 cgcctcaaca ggcttccatc cgcgacgccc tcctccggct ggagaagggg gagccgcccc
     5341 aatatatttt gggcaaaacc tggttttacg gtttggaact gaaggtcgat ccccgcgttc
     5401 tcattccccg gccggaaaca gaaggcctgg tgaaactcgc ccttgagcgc ttgaaacctg
     5461 aagcccgggt gctggagatc ggcaccggaa gcggcgccat cgccattgcc ttgaaaaaaa
     5521 ccgggccaaa cctgagcgtg atggccacgg atatctcttc cgcggcgctg gaactggcgc
     5581 gggacaacgc tttccgacac aactgcgcca tagatttcgt agaggcggac cttttccctc
     5641 ctggcgactt ccgctttgac cttgttgtca gcaatccccc ctatatcagc gaaaaggaat
     5701 actcggaact ggaacccatg gtgcgggatt atgagccgcg gcaggctctg ctggcagcgg
     5761 aagaaggcct ggaatattac cgctaccttt tttccggtct acccaaccaa ctgaatgatg
     5821 gaggcctggc cctctttgaa cacggagctt tgcaaagaca gagcattatt gccctggggg
     5881 cggaagccgg ctttgaatgc tgcctggcca aagatgacct cgccggccgg aaccgctatc
     5941 tgggctttag acttaaacga aatacagaga aatagagaat aaggacacta aatggacaga
     6001 ttcatcattg aaggcaaccg cgaactgagc ggcaccatcc aggtgagcgg ggccaaaaat
     6061 gccatcctgc ccgtgatggc agcctgcctc ctcgctccgg gcaaatccgt gctgcgcaac
     6121 gttcccaacc tcatcgacct taaaaccatg gcccacctgc tgcgggtgct gggagctcgc
     6181 gtggtctatg aaaacggtat gatgtcgatt gactcacgag atgtgaattt caacgaagcg
     6241 ccctatgaac tggtgagcaa gatgcgcgct tcaatctatg tgctggggcc gcttttggca
     6301 agattgggcg aggcacgggt ctcatttccc ggaggctgcg ccatcggcac caggcctgtg
     6361 gacctgcatc tgcaagcaat ggaagcactt ggagccaaaa ttgagatcga acacggctac
     6421 atccacgcca ccgccgaccg gctcaccggc gcggacatcc atttcgctaa atcctctgtg
     6481 ggagccacta tcaacgccct catggccgcc gtcttggcca gtggcaccac ccgtatgttc
     6541 aatgccgcca tggaaccgga ggtggattcc accatcgaac tgctcaacaa aatgggcgcg
     6601 aaaatttccg gtcagggcag caccaccctc accatcgaag gcgtggacga cctcttccct
     6661 gtggaaatga ggatgatctc cgaccgcata gaagccggca ccttcctcat cgccggagcc
     6721 cttagcacta agcctgtcac cgtggcaaac tgccagccgg agcatttggg catcctgctg
     6781 gaaaaactgc gcgccgccgg ctgcgaaatc gatgtccaag cttccaacat caccgttgtc
     6841 ccgccccaag tgatcaaacc tgtggacatc ctaaccctgc cctatcccgg cttccctacc
     6901 gacctccagg cccagttcac cgtgctgatg gcgcttgccg aaggcacctg cttcatcgag
     6961 gataccattt tcccggaccg cttcatgcac gtcgccgaac tcaaccgcct ccaggccgac
     7021 atccgcatgg aacgcaacgt tgcccatgtg aaaggggtca aagccttcag cggcgctgaa
     7081 gtgatggcca ccgacctgcg cgcctccgcg gctcttgtcc tggccggtat ggtcgctgaa
     7141 ggtaccacca ccgtttcccg catctaccac atcgaccgag gatacgaccg tatcgaggaa
     7201 aaactcaatt ccatcggagc ccgcatcacc cgcgaaatgg cttgattgga ctgtctataa
     7261 atcaggttct ctttcagaca ggataggtta acccggttta gaagagagcc tgatttattt
     7321 gtggactaaa ctgccctcaa cctcaaatct gacaattccc tggctccctt tccccgcacc
     7381 tcagtagggt aaattagcga acctatgaat ttgccctcgc gggtcaggtc cttagcgctc
     7441 gcttccgttc cactcccacg cctcccggat aattcccgca ccaaatgcgg ccatcgtgcg
     7501 ggaggcgaga caggggtatg tgaacaaggg ctaaggacca aacgccctcg cggtcgcggc
     7561 cggtatccag accgggctgt tgcgatcaca ggaatcatcg gctcaagggc aggcttggat
     7621 acgaaaaaca cccgcggcga tgcgggggtt tgatatggct caaattcatt tatggtgggt
     7681 acccgtgatt ctgctttatc aacacttcag agaagcctgg tatccattct ttcttaacct
     7741 aatgataata atatcagtaa tagtgattat agtcatgctg tatccaagtc tgcgatcgga
     7801 tgacggcgtt tctaaccact cggtttgaaa gagagccata tctatccctg ttttctcctt
     7861 accctggggt tatgccaaag gtcttatggt gtcggcaagc gtgaacggct ttggctggtc
     7921 actccggcag gtggtcccaa agcccttcat agctgtccca ttttccgttt ttgagggtga
     7981 gggtgatggt gcgcgcccgg ttgcgctggt cctcggtcat ccactgcgcc caaagctggc
     8041 ttaccacata attggcaatg ggttgcggcg ccggctcgta acccacccaa aggaatttcc
     8101 gtcccaggga acgcagcttt tcagctaatc ggatgctgtt ttcctggtcg tggttgccga
     8161 ttagaaagat ctcgaagccg gttgtgtctg ccggggttag caggtccgta agttcgtaat
     8221 agacaccatt gttccagccg cgggggtaat ccttcagaaa gatctccacc gtgaggctgt
     8281 catccaggtc cagatgtttg gtttggaaag cggtcaggct gtccgagatc cggtaaatgg
     8341 cggaaaggta gcattcaccc ttgggcacct ggaaactgag gaccccgttg ccctcgtccg
     8401 ttccagaata gccttccagc caagcatagt cgccctcgcg gatatcagag aggatgagca
     8461 tggccctttc gttcgatccc agaggcgcgc ctgattcgtc agaaacaagc acggtcagct
     8521 tcactaatga ctcctgctca tcgggatcta tctccggagc acaggtttcc aaatcataat
     8581 aaccgctgtc accatcgtca aatctgaccc ctatgtgctt gctccggaag ccggcatcgg
     8641 caggaattcc gttggcacgg gccagactga cagccatcag cttgtattgg gcgttgttga
     8701 gatacttttg cccgatgacg acatggagcg gcggcatacc gcttaaagcc ttgtcggggt
     8761 ctatcttgta gtttctcttc agccagcgcg ccgcccgatt cagacgttca agccgggttt
     8821 caccctgttg gtaaaatgag cggggataga agctgggtat cccgttccgg aaccccagcg
     8881 gttggggcag ttcttcatag cggacagagg gaaagatcag cgaccccata tcctggccaa
     8941 tattctcatc atcttcatat tttaggtaag catgatatac tgcttcaatc tggtcagcgc
     9001 tggcctgcca gaggaactta gggtccatga tgccccattc ttcagccaaa caatcctcga
     9061 taaacccttc gcagggatgg ggatagcggc gcaggaactc caaagccccg gcataattcc
     9121 cccgcgcagc tttgacaaat tccgcgttga gtgaatccgc ctgggaagcg gcgaccctgg
     9181 cccagcttcg gtcgatggca ttccagcgtt ccttttcccg cttcacgcct tcgttccagt
     9241 attccggagc ctgtttccat tcgaaggggt tggagggata gacaagccgg tcatcgcggt
     9301 cagccagcgg cccggctttc agcgtcaggt cgcagttgat aagcgtgctg tcgctggaag
     9361 ggaccagctc caaggcccgc ttcccatctt gctcggcggc caggtaaaag gcaccgcggc
     9421 ccacggagaa agctaatttg ccttcggcat ccgttttgag ccaggccagc ggacgcaggg
     9481 cagaccagtt caccaccatc acgccgaccg aggtattcgg aagcggatta ccttgctcat
     9541 caagggtttg gatgctgaga gaacgggtgc gttccccggc atagttgcgg atggaattga
     9601 tcacgcaatc gtattttccg cgaatcagga cctcgtcgct ggctgcgggc agagagccgt
     9661 cggcaaggat cagcacggtc ttgtcgatga ggccgctgaa ccaggtctgg tcgggccaat
     9721 aggcggcatc catgtctccg gtgtagtgcc aggctccttc cagccaaact tctgcccagg
     9781 cgtgattgtt atcctgatgc ggccaccagg gtgtggaagc cggccgcgag ggcaatccaa
     9841 ccgttctggc ggcggccacg aagaggattt gcatctcctc acaacgccct aacaggcttt
     9901 tttgggtgat gtcgagcggg gtttggtcgc gtcctgaggt gggttggaac ttgaggcggc
     9961 ccacgcacca ctggctgact tttctgtaca ggtcgatctc atccgtgctg gactccatga
    10021 tttcacgcag accatcccgg agcaggcctt cgcggtaagc ctctatgcgt tcgtcggata
    10081 cggtttgctt ggccacgtag gagaggtaga attccgggtc caggctggtc ccgtgggtat
    10141 tgaggagggc gcgtacatgc agatagttcg acagcacatc tgccgggcgg gcgatctgga
    10201 ggttggcatc actttcatag gcaagcagat aggccatcag aacgtcgtct tgttctttca
    10261 gcagacgccg atattccttc ctttgatggc ggtccaactt ggcaaggttg cccaacgctt
    10321 tggagcgcag ggtcttatac agcccggggt tccccttgga ggtgaaggct gaaggaagag
    10381 cggccaggct tgcaatggca caggccagaa aaactataag cagggtgaaa cgcttggcgt
    10441 tcatcatgat ttacctctca aacacgttaa taaaaaatcg ggattatagc ataaaatcca
    10501 atcggtcatc ccgacaactc agcggaacaa ggatcattgg acccgttgga atttcaaaat
    10561 gctccagcag gctattcagc cagttttagc atcttgccgc tgagggagtt ctcaccagcg
    10621 ctgaaccgcc agaaatagag cccggatgaa accatttctc cccgggtatc gcatccgtcc
    10681 cagagcagtt catgggctcc agccgtcaaa gtcaagtaac tgcgggagta taccttctgc
    10741 ccccgaatat tgtagatctc cagcctggca gcagaggcct tgggcagttc tacctgaagg
    10801 gtcatcccgg cggtgaaagg attgggaaag ggccttgcgg acagctctgg agcaggaact
    10861 tggggatcgt tggcgtccgt gggcaggtaa tctatcacgc ccaggcggtc aaggatggtg
    10921 cccagcagga agcggaaatc gctggagtag ggtggggtgt cgtccacatc ggccaaggca
    10981 aaggacgaag cgacggtggc atagttgtct gtaacgttca gtatggcaac cggatggtca
    11041 ggatgctgaa gattttgggt gctgatgaaa acctccgagg atgagttcgt gggcatcagt
    11101 atctgggtat gggggtcagc ctgggcgtca tagccccaag tgtaggtgtg gccggcctgg
    11161 gtggtggcga ggtcttcgat gtaggcaaag ttttccaggg atgcctgggt ggcgaatttt
    11221 ccccagaagg ggtcttggga gtcccagctt acatcacctt ccagatagag tttgccgccg
    11281 ctgttcagat aggaattcag cagagcgtaa tccagcgaat cagggttcag gacataggct
    11341 gtgtcgcccc agccgaagag gcagaagacc gcctcgaact gattgatgta gtcatgatcc
    11401 gggattagct tatcggtgta caccacattg aaatgggcga ggtcgagcgc ttcgacgatg
    11461 ggcaggccgt cctggtgaga gggttcatag accagtatct cgccattgct gtagttctgc
    11521 tggccgatgt aaccgatggc gggatcaccg taaagcagat aggtatagac attttgcagt
    11581 tccggccaga tgatcccgcc ggcatcgacg gggttgccga agaggtaata ctggcagtgg
    11641 gtgaggttgg caaaggcctg ggcggcaccg accgtcatgc cgttgctggc gatgttttcc
    11701 agccagtggt aattgtagga gctgagcccg ccccagccgg gattggccca gccgatcttg
    11761 taccagccgg tcctggtggc agcggtgacg gcaacggcct ttttctgtag ggcgtattca
    11821 gccaggcatg gctggtagct gtctatcgcg ccgttgtagc aggaggcagc gaaaacgacc
    11881 atgccgtcgg ggttggcaag gttgttaaag ctctggcggt ccaccatgcc catccagttc
    11941 atttcccagg aatcgggcag gccgttgttg ttgtcatcgt tcatccagac cttgcgggaa
    12001 gaggatcccg aagagccgtg ggcgctccag ttgagcagcc cgtagctgtt tgtattcaac
    12061 tcgtttttga gcaggtcgta atccagatca atatcacagg gataggaggg caggaaacca
    12121 tatcgctcat acattgtggt acaatcataa tcgctgagga tggtctggcg ggctaactcc
    12181 ataaaggtcg cgccatcggt ttgcagatag atggtttccg gctcgccgcc gtagttcaga
    12241 taagcggctg gcagcagggc cttccgcttc cagggtgcat tggtttgttc ataagccacg
    12301 gtgcgcgcgg cgatctgggc cacggcaacg ggactgttgt tggaaatgcg gcccacaaaa
    12361 acttccgggg taaaatcaca gagaaagtcc tggattccct cgccggagga atattctccc
    12421 aagcggccgt cggagtcggt atccacaatg ctgctcaggt cgccgtagaa gaaatccgag
    12481 gccactgtct cgctgccgtc cggctcggga gtaagataca tcaccggaac tttgtcgtaa
    12541 tcgcccacca gcagcagata ggaaaaggga ttgctgtgat actgggcaat aagatggttg
    12601 cgcagctttt cgccagctgt ggcgccgggg ctggaattga ggatggtgtt gatatccgcg
    12661 aaagcggtga taaggccctg cccctggcgg taagtttcca gcggggcaat cgcagtataa
    12721 agctccgggg tggagacgat cagatagtcg tagtttttgg cagagctggg aacgtaatac
    12781 ttgtttaggt cctggggatt gacgaagaaa tcagcaggtt caactccttt tttggcaagc
    12841 tgttgccaga caggaggcag tgtt
//
LOCUS       DAOOWR010000009        10880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6021_length_10880_cov_4.131917, whole genome shotgun sequence.
ACCESSION   DAOOWR010000009 DAOOWR010000000
VERSION     DAOOWR010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_10880_cov_4.131917"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLB85_00590"
     CDS             <1..491
                     /locus_tag="PLB85_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71852.1"
                     /translation="AYAISPGADVWLRMVELSAANNWPEFDTIWKSGSQFQADHPQTQ
                     LLRVEYFFHTGSLADAKALADSLLDSQTNPWIRAGAEIWLGRISLEEGDYHSARRGFQ
                     KIRLLYQDFPEVHSEACHYYIISLVKLGETHEARLALTEYRHLFSDVQADEIEALLNN
                     GR"
     gene            498..1988
                     /locus_tag="PLB85_00595"
     CDS             498..1988
                     /locus_tag="PLB85_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU71853.1"
                     /translation="MRKNLATVLTALFLVCVTLSAQPQALPSVEIGSDVAVKAPLVKK
                     PLPYPGETPTDSIPPMIPPVFSLKHRPRALPQGTLRPLRLELSSGTDFETKLAASCYP
                     SLRFIPLLRLDADLWVPGTNVSRSTLNASLQTDFDPELRFNHLFSWQKAKTRSFAAGA
                     FSYSIANLYSEIAIHDIYFSNLKTDLSLEEVEQNLAGASQADFAIGIRHSHDLELHKL
                     KFTNRLVLQDTAFGLSVQYHAPWLEEHLPELKLGLMTDFIHVLPVIDLHRRFLLGRGK
                     YLELSNRSEFQNLTFQRLRELYPWTVLPERERVVMTPLNLSLNGWQAFNDEDALFRLL
                     GFRQNLRFSYNQPQLYVRDVSGQTWLRQTDILSYRLGAEASMQFWGWIIDQDLNLNLE
                     YLQDERWRRRPFSPIFNALTEARHNLGELELLAQLDQQYWRRDEFDQPLSAVFDLSLG
                     LSLPLRSGLSLTAGLNNVFNSKHGNFGDLPNPGRSFHVSFSYLPLR"
     gene            2244..3011
                     /locus_tag="PLB85_00600"
     CDS             2244..3011
                     /locus_tag="PLB85_00600"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU71854.1"
                     /translation="MRKLDLQQERARITGFIREYLKSAGFARVIFGLSGGVDSAVCAA
                     LAVEALGKENVIAAIMPYRTSQPESLADAGLLAGILGIEHHVIDITPMANAYFTTYEP
                     EADKLRIGNWLARTRMCVLFDLSVKHRALVMGTSNRTELMVGYVTQFGDAACAFEPIG
                     HLYKTEVWELAKLLDIPEKIINKTPTADFWAGQTDEADLGLTYPVLDEILYALTELDV
                     NVDASENLEHPAELYRKVERMITASAFKRRLPPVLEN"
     gene            3014..4189
                     /locus_tag="PLB85_00605"
     CDS             3014..4189
                     /locus_tag="PLB85_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71855.1"
                     /translation="MLLNRERCFKCRAERSVRKCPRNRDKLIGWKCCLELRIDLRCPS
                     ECPYSAVKDENSPFPAFRADSNTEFTRTAKRHIDLWIYQPQEGLDGLSPADYAAKDSA
                     GMLAWLGGFQFPANFPMTYLLQKLQLPHDEYEEPETPEMVAFGFFDAVITQDWQKLRA
                     FTINDREDRDLAERYTRLVSEIPELKKVNRYEILHAGAADDGVSAMVVLDLGGKLIWA
                     VLLTSAEGRWKVRQNLNGGPHLYYGQNKLFHKLAEHLAQGEAEAAWDLIRKNLPLYPD
                     CADLYYYRALYWQLARQFDKAKEDLRNSLALDNHFFAAGFALSALYLNDRELEQAKEL
                     LSWLAADRPDDLNVRNNLAACEAGLGNISAAQAIWRELLKIAPNYEPALKNLERYPG"
     gene            4149..5297
                     /gene="ribD"
                     /locus_tag="PLB85_00610"
     CDS             4149..5297
                     /gene="ribD"
                     /locus_tag="PLB85_00610"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU71856.1"
                     /translation="MNQPSKTLNATQAEQAKWMKRAIRAAEKARGTCSPNPFVGAVIV
                     KNGKMIAEGWTQSYGGDHAEVQALAKAGKDARAADMYVTLEPCSHQGKTPPCTQAIIT
                     AGIKRVYLGILDPNPLVHGKGVLQLQEAGIEVFSGVLAEEISRQLEYYFCRVHNRRPF
                     VIWKAALSLDGKYAAQDGSSRWISGERSREQVHRLRQEADVVLTGIGTALADDPLLNV
                     RLKNAFKQPLRAVLDPLLKLPPDSRIARSMNDFPTLVFCARGKEHTMRAKILAALGAK
                     IQPVTAIGNILSLQEVLGILHQQGHCLVLLECGSKLASAFFAARLVDKCLIFYGPKLL
                     GGNKAMLAELNLPDISSALNLQDLSCKSSGEDLMVSAYPVYDWHGDSS"
     gene            5366..5761
                     /locus_tag="PLB85_00615"
     CDS             5366..5761
                     /locus_tag="PLB85_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU71857.1"
                     /translation="MKIVHFDKVALEPVNAEGAEGTKIRWLIGQKDGAPNFALRLFEV
                     EPQGHTPYHVHDWEHEVYVLSGIGVLQTENGELTFKANDAIYVDPGMPHAFKNTGTDL
                     MKFLCIIPHEQPVLKKTLNPFADETANNC"
     gene            5771..6832
                     /locus_tag="PLB85_00620"
     CDS             5771..6832
                     /locus_tag="PLB85_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOU71858.1"
                     /translation="MPNAKNLKFLKDLVSAPSPSGFEGPAQKVVRDYLKPVADKITID
                     RNGNLIALKKGSGKLKVMVVGHADEIGLIVNHIDEQGYIYVKSLGGFDVNLLPGLRLD
                     IHHQGKVVRGIIGKKPIHMMRGSDESPKLKMEDLWIDIGVSSKEEALKKVAIGDVIIY
                     HSEFEQLSDDLIVSKATDNKVGVYVAAVVMQELAKKQITASYYAVSSVGEETTMRGAR
                     TSAWQIEPDIALVVDVTFTSDIPGTDKRVIGDISLGKGPTLSIGAALHPAVHKRLLEI
                     AKKHKLPIQMEIAPGRTGTDADAIHDLKSGAATAVIGIPNRYMHSPNEVISLKDLDTA
                     VKLITEFVLSLDDKFKLER"
     gene            7246..7425
                     /locus_tag="PLB85_00625"
     CDS             7246..7425
                     /locus_tag="PLB85_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71859.1"
                     /translation="MSKDNRRGKGVKNLPNHGRGECPVCHRTGIKLLTEIKVGEKTVK
                     VCKNCKNIAAARLSA"
     gene            7496..8269
                     /gene="rsmA"
                     /locus_tag="PLB85_00630"
     CDS             7496..8269
                     /gene="rsmA"
                     /locus_tag="PLB85_00630"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU71860.1"
                     /translation="MRAIKALGQNFLNQPEIARKIVELAGIQPGETVWEIGPGHGILT
                     RALLAAGARLRAFELDRRLEAPLRDEFGDSLELVMGDVLKQDWDKALAECCQPLKLVA
                     NIPYNITSPLLARLETHHQAFTSATLMVQKEVAERVCASPDCKAYGVMTLRLRRIFDA
                     EILLDVPRKYFSPAPNVDSAVLSLKPRAVPAVIPDLAKYLKLIELAFAHRRKTLRNNL
                     ASLLDRDGLNRLQQQSGIDLTRRGETLSEAEFIALSSLL"
     gene            8247..10016
                     /locus_tag="PLB85_00635"
     CDS             8247..10016
                     /locus_tag="PLB85_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71861.1"
                     /translation="MLSVLCFSCCLLASQVNLLDFSYSSSNASRNLENNLRCQLAPFK
                     ASLMQINAQSFGEQRLNFSQRSRRSLLNLDLAFDRGLFRHNVQSGYEYLYDSSELDED
                     FQPYRNKTGFLGYSLEIQPADSLLFEAGVQGLIRSEEDRYLLDSKINSQGYLVHGRAT
                     ASTGLWNSRAGLNAAFERKTMDWEFYQTGSLSAWLNHQSQSLLFNNNFSLSRRYDDIY
                     VLLPDVGGRGHYDLDDRQKRSSLVYSGSLEYEPWEILRLSLLENFSRKITDLAQNTMR
                     NNTDLENQASLSLELKPWSRLGWTSTFSHGYGLKTFAQAQTSRQLENRFLSSRLNWEY
                     TDGDTLSAAFAVDLQRSTFPDDQNRWDNDLRHIRLNFTNAHYWKDRLKISNGFFWNIT
                     DDVYLDGLLSCNNKQTSSYVYNPSCALLIGDRLLFNQNYLVRADYTSYMYDPTDKAFY
                     RQLEMEYKLVFDSFPFAARSQDQRWLLLPYRTNDGNALLADLSFGFERNEYADYNGQE
                     YVIGFKNTRYSAAFTLKHDIQDLYYIIQPRYTWGTWIEYNLLLGLAWKFTDSSLLEFS
                     LNPVGDSLSNLDWRSSVSLGARF"
     gene            10016..10468
                     /gene="tadA"
                     /locus_tag="PLB85_00640"
     CDS             10016..10468
                     /gene="tadA"
                     /locus_tag="PLB85_00640"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOU71862.1"
                     /translation="MSAEHSAWMKLALEEANLARSEDEIPVGAVLVKGGELIAAEHNR
                     TRQLKDPLAHAEKLLIDKILASKIKYLQDYTLYVTLEPCLMCAGMMIWSRLGTLVYGA
                     SDPKAGAVGSIYNVLADKSFNHHPRVLRGVLEADCAALLRDFFQSRRQ"
     gene            10472..10561
                     /locus_tag="PLB85_00645"
     tRNA            10472..10561
                     /locus_tag="PLB85_00645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10506..10508,aa:Ser,seq:cga)
     gene            10569..>10880
                     /locus_tag="PLB85_00650"
     CDS             10569..>10880
                     /locus_tag="PLB85_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71863.1"
                     /translation="MNQTDTQLVCDSGCPLCGGGTLREELLLYNIPTESYHALQVEFA
                     SLTGKKLSPDSDVSILSGGQKVLLMCLCALFSPARKILFINLWHSLDAVNRARTKELI
                     TL"
BASE COUNT         2592 a         3112 c         2795 g         2381 t
ORIGIN      
        1 gggcctatgc cataagcccg ggcgccgatg tatggctgcg catggtggaa ctctccgccg
       61 ccaacaactg gcctgagttt gacaccatct ggaaatcagg aagccaattc caggcggatc
      121 atcctcaaac tcagcttctg cgagtggaat atttctttca caccggcagc ttggctgatg
      181 ccaaagcctt ggcggattca ctgctggaca gccagaccaa cccctggata agggcgggcg
      241 cggaaatctg gctgggcagg atcagcctgg aggaaggcga ttatcactcc gcccgccgtg
      301 gttttcaaaa aatcaggcta ctctatcagg attttcccga agtccattcc gaggcttgcc
      361 attactatat aatcagcctg gtcaaactgg gcgaaaccca cgaagcgagg ttagccctga
      421 ctgaataccg gcacctgttc agcgatgtgc aggcagatga aatcgaagcc ttgctgaata
      481 acggcaggta ggggcagatg cgcaaaaacc tggcaactgt cctgaccgca ttgtttctgg
      541 tctgtgtgac gctttcagcc cagccccagg cgctgccttc agtggaaata ggttcagatg
      601 tggcagtgaa agctcccctg gttaaaaagc ctctccccta tcctggcgag acccccaccg
      661 acagcatccc gccgatgatt ccgcctgttt tctccctcaa gcacaggcca agagccctgc
      721 cccaaggaac tttgcgccct cttcgtctgg aattaagctc cggaactgat tttgagacca
      781 aacttgccgc gtcctgttat ccatcattga ggttcatccc cctgctgaga ttggatgccg
      841 acctctgggt gcccgggacc aatgtctcgc gaagcaccct caacgccagc ctgcaaacgg
      901 atttcgaccc cgaactgcgt ttcaatcacc tgttctcctg gcaaaaagcc aagactcgga
      961 gtttcgctgc cggggccttt tcctacagca tcgccaacct gtatagtgaa atcgccatcc
     1021 atgacatcta cttctcaaac ctgaagaccg atctctcttt ggaggaggtg gagcaaaacc
     1081 tcgccggggc ttcccaagcg gatttcgcca ttggcatcag gcattcccac gatctggagc
     1141 tgcacaaact caaattcacg aaccggctgg tgctgcagga tacagccttc ggcctttccg
     1201 tgcaatatca cgcgccctgg ctggaagagc atctgcccga actcaagctg ggcctgatga
     1261 ccgatttcat ccacgttttg ccagtcatag acttgcaccg ccgcttccta ctgggtcggg
     1321 gaaaatatct ggaactgagc aaccgcagcg agtttcagaa cctgaccttc cagcggttgc
     1381 gggagctgta tccctggacg gtcctgcctg agagggaacg agtggtgatg acgcccctaa
     1441 acctttccct gaacggctgg caggctttca acgatgaaga cgccctcttc cggttgctgg
     1501 gttttcgcca aaacctccgc ttcagctaca accaacccca actctacgtc cgcgacgttt
     1561 cggggcaaac ctggttgcgc cagactgaca tcctgagcta ccggctgggg gccgaagcca
     1621 gtatgcaatt ctggggctgg attatcgacc aggacctgaa ccttaatttg gaatacctgc
     1681 aggatgagcg ttggcgtcgc aggcccttct cccccatctt caatgccctt accgaggccc
     1741 gccacaactt aggcgaactg gaacttttgg cccagctcga ccagcaatac tggcgccgcg
     1801 acgagtttga ccaaccgctt tcagccgtct ttgacctcag cctcggattg agcctgcccc
     1861 tcaggagcgg cctgagcctc accgctggcc tgaataatgt tttcaactcg aagcacggaa
     1921 atttcggcga cctgccaaac ccgggccgaa gcttccatgt ttccttcagc tatcttcccc
     1981 tgcgctgatg gattgttctg gatagctgtt tagatgaatt cccgcagaaa tcccatttgt
     2041 tgccgactct gcccgtcctt aacgccatcc atttcactcc ccgtttgcct ccccatgagt
     2101 tcccgcctga aatgcgggaa ttgtgcggga ggcgaaaagg atgcggatgg tatagggcta
     2161 agggcgaata aagttttatg cttgacctaa attgaacaga agaaattatg ccgatgcatg
     2221 gataacgcat ctggaggtga ataatgcgta aactggacct gcaacaggaa cgggcccgga
     2281 ttacaggatt tatccgcgaa tatctgaaaa gtgctggttt tgccagggtg atcttcggac
     2341 tatccggcgg cgtcgattcc gccgtgtgcg cggccctggc agttgaagcc ctgggcaagg
     2401 aaaacgtgat cgcggcgatt atgccttaca ggaccagcca gccggaatcc ctggccgacg
     2461 ccggccttct ggccgggata ttgggaatag agcaccacgt aatagatatc acgcccatgg
     2521 ccaacgccta ttttaccact tacgaacctg aagccgacaa actccgcata ggcaactggc
     2581 tggcccgcac ccggatgtgc gtgctttttg acctcagcgt caaacaccgc gccctggtta
     2641 tgggaaccag caaccgcacg gagctgatgg ttggctatgt cacccaattc ggcgatgccg
     2701 cctgtgcttt cgagccgatc ggccacctct acaagaccga ggtctgggaa cttgccaaac
     2761 tgcttgacat tccggaaaag ataatcaata aaacaccaac agcggacttc tgggccggac
     2821 aaaccgacga agccgatctg gggcttacct atccggttct ggacgagatt ctttacgccc
     2881 tcactgaact tgacgtgaac gtggatgctt cggaaaactt ggaacatcct gccgagttgt
     2941 atcggaaagt ggaaagaatg atcacggctt cagccttcaa acgcaggctg ccgcctgttt
     3001 tggagaactg aacatgctgc tgaacagaga acgctgtttc aagtgcagag cagaacggag
     3061 cgtgcgcaaa tgcccccgca accgggacaa gctgattggc tggaaatgct gtttggaact
     3121 gaggatcgat ctgcgctgcc cctccgaatg cccttacagc gcggtgaagg atgaaaacag
     3181 ccccttcccc gctttccgag ccgattccaa taccgaattc acccgcaccg ccaaacgcca
     3241 cattgacctc tggatctatc agccgcagga agggctggat ggactcagcc ccgccgatta
     3301 tgctgccaag gacagcgctg gaatgctggc ctggctcggc ggattccagt ttcctgccaa
     3361 tttccccatg acctatcttc tccaaaaact ccagctgccg cacgatgaat atgaagaacc
     3421 ggaaaccccg gaaatggtgg cattcggatt cttcgacgcc gtgataaccc aagactggca
     3481 aaagctgcgc gctttcacca tcaatgaccg ggaagaccgt gacttggcag aacgctatac
     3541 caggcttgtt tccgagatac ctgagctgaa aaaggtgaac cgttacgaaa tcctgcacgc
     3601 cggggccgcc gatgacggcg tgagcgcgat ggtggtgctg gacttgggcg gaaagttgat
     3661 ctgggcggtc ctgctcactt cagcggaagg ccgctggaaa gtccgacaga acctcaatgg
     3721 cggtccccat ctctattacg ggcaaaacaa acttttccac aaactggcgg aacaccttgc
     3781 tcagggcgaa gccgaagcgg cctgggacct aatcaggaaa aacctgcccc tctatcctga
     3841 ctgtgctgat ctctattact accgcgcgct ttactggcaa ttggcaaggc agtttgacaa
     3901 ggccaaagag gacctgcgca actccctggc cctggacaat catttcttcg ctgctggttt
     3961 cgcgctctcg gcgctttatc tgaatgacag ggaactggaa caggcgaagg aactgctttc
     4021 ctggctggct gccgaccgcc ccgatgacct taatgtgcgc aacaatctgg ccgcctgtga
     4081 agctggcctc ggtaacattt ccgccgccca ggccatctgg cgtgaactgt tgaaaatcgc
     4141 tcccaactat gaaccagccc tcaaaaacct tgaacgctac ccaggctgaa caggccaaat
     4201 ggatgaaacg ggccatcaga gccgctgaaa aagcgcgcgg gacctgttct ccaaacccct
     4261 ttgtgggagc cgtgatcgta aaaaacggca aaatgatagc tgaaggctgg acccagagtt
     4321 acggcggtga ccacgccgag gtccaggccc ttgccaaggc tgggaaagat gcccgggctg
     4381 cggatatgta tgtaaccctg gaaccatgct cccatcaggg aaaaaccccg ccctgcaccc
     4441 aggccatcat cacggctgga atcaaacggg tctatctggg aatcttggac cccaatcccc
     4501 tcgtgcatgg caaaggcgtt ttgcaactgc aagaggcggg cattgaagta ttctcaggag
     4561 ttctggcgga agagatttcc cgccagttgg aatactattt ctgccgcgtt cacaatcgcc
     4621 gtcccttcgt gatctggaaa gctgcgctgt cactggacgg taaatacgcc gcgcaggacg
     4681 gctcttcacg ctggatcagc ggggagcgtt cccgtgaaca ggtccaccgt ctgcgtcagg
     4741 aagctgatgt ggtgcttaca gggattggca cggccctggc agacgatccg ctgctcaatg
     4801 tcaggcttaa aaatgctttc aaacagcctc tgcgtgccgt cttagaccct ttgttgaagc
     4861 tgcctccgga ttcacgcatc gcccgcagca tgaacgactt tcccactctt gtcttttgcg
     4921 cgcggggcaa agaacacaca atgcgggcca aaatccttgc cgccctgggc gcaaagatcc
     4981 agccggtcac cgccattggc aatatcctca gcctgcaaga ggtgctgggc atccttcatc
     5041 aacagggtca ttgcctggtg cttctggaat gcggcagcaa actggcttcg gcttttttcg
     5101 cggcgcgctt ggtggacaaa tgcctcatct tttacgggcc caagctgttg ggtggaaaca
     5161 aagccatgct tgcagaacta aacctgcccg atatttcatc cgctctaaat cttcaagacc
     5221 tgtcctgcaa atccagcggt gaagacctga tggtgagcgc gtatcctgtt tatgactggc
     5281 atggcgacag ttcctgatta tcctttaatc aaatgccgtc ctaatggcag aaccttttat
     5341 taacaagcta ttcaaggagg aatagatgaa aatagtacat tttgacaaag tcgcccttga
     5401 acccgtcaac gctgaagggg ctgaggggac caagatccgc tggctgatcg ggcagaaaga
     5461 cggcgctccg aatttcgccc tgcggttgtt tgaagtggag cctcaaggcc acacgcctta
     5521 ccatgttcat gattgggagc acgaagttta cgtgctttcc ggcatcgggg tgctgcaaac
     5581 cgaaaacggc gaactgactt tcaaggcgaa cgacgccatc tatgttgatc ccggtatgcc
     5641 ccacgctttc aagaataccg gcacggacct gatgaaattc ctctgcatca ttcctcacga
     5701 gcagccggtg ctgaaaaaaa cactcaaccc cttcgcggac gaaaccgcca acaactgtta
     5761 ggaagtgaag atgccaaacg ccaagaacct gaaattcctg aaagacctgg tttccgcgcc
     5821 cagcccctcc ggctttgaag gccctgccca aaaagtggtg cgtgattatc tgaaacccgt
     5881 ggcagacaag atcacgatcg accgcaacgg caatctcatc gccctcaaaa agggtagcgg
     5941 caagctgaaa gtgatggtgg tgggccatgc cgacgaaatc gggctgatcg tgaaccacat
     6001 tgacgaacag ggctatatct acgtgaaatc cctcggaggt tttgatgtca atttgctgcc
     6061 cggattacgt ttggacatcc atcatcaggg aaaggtggtg cgtgggataa tcggcaaaaa
     6121 gcccatccac atgatgcgcg gaagcgacga aagccccaag ctgaaaatgg aagacctctg
     6181 gatcgacatc ggcgtttcga gcaaagaaga agccctgaag aaagtagcca tcggagacgt
     6241 tatcatctac cacagcgaat ttgagcagct ttccgacgac ctgatcgtct ccaaagccac
     6301 tgacaacaag gtgggggtgt atgttgcagc cgtagtgatg caggaattgg caaagaaaca
     6361 gattacagcc tcttattacg ctgtttccag cgtgggagag gaaaccacca tgcgcggcgc
     6421 ccgcaccagc gcctggcaaa tcgagcctga catcgccctg gtggttgatg ttactttcac
     6481 ttccgacatt cccggcacgg acaagcgcgt gataggagac atttccctgg gcaaaggccc
     6541 cactctcagc atcggcgcgg ctttgcatcc tgctgtgcac aaacgtctgc ttgaaatagc
     6601 caaaaagcac aagctcccca ttcagatgga gatagcccca ggccgcacag gaaccgacgc
     6661 tgacgccatc cacgacctca aaagcggtgc cgcgactgcc gttatcggaa ttcccaatcg
     6721 ctacatgcat tcgcccaacg aagtgatttc tctgaaggac ctggacactg cggttaagct
     6781 tatcaccgag ttcgtgctct ccctggacga caaattcaaa ctggaacgct aaacaataca
     6841 gccttaacaa ataaacatcg aagcccgctg aggaaccctt cggcgggctt tgcatttatt
     6901 aactcgatct tattgggctc tctttcaaac cgggtgaata acgccttgaa gcggaggatg
     6961 atagcgtcgg tgtgaagtag ctggataccc cgcaaaggag cgaagctaag ccatcaggct
     7021 gttaaggccg tttcatgtga ccttgacgcg cctcccgaac aattccctcg ttcaatgcgg
     7081 gaatcatacg aggggtttag gaaagggatg aaagcaggcg ttcaggtcgc gactgtcagt
     7141 tattaacgac gggttttatg ataatatctg ttgacgcaaa agccgccacg gtatttttgg
     7201 gtctgttata gcaaaaaata atgtgaataa cccaaggaga tagtgatgtc caaagacaac
     7261 cgtcgtggca aaggtgtgaa aaacctcccc aatcacgggc ggggtgaatg ccctgtctgc
     7321 caccgcaccg ggatcaagtt acttaccgag atcaaggtcg gcgagaagac cgttaaagtt
     7381 tgcaaaaact gtaagaacat cgccgctgcg cgtctcagcg cctgacgcgg ccactttcta
     7441 tagattcaga tcgccccttg acaagctttt ggggtgatct gaattttttt gagcaatgcg
     7501 cgccatcaaa gccctcgggc agaattttct gaatcagccg gaaatcgcgc gaaaaatcgt
     7561 ggagcttgcc ggtatccagc ctggagaaac ggtttgggag atagggcccg gccatggcat
     7621 tctcaccaga gccttgttgg cagccggggc aaggctgcgc gcgtttgagc tggacaggag
     7681 gttggaagcg cctttgcgtg atgagttcgg cgattccctc gaactggtga tgggtgacgt
     7741 gctgaagcag gattgggata aagccctggc ggaatgctgc caaccgctga aactggttgc
     7801 caacattccc tacaacatca cctctcctct tttggccagg ctggaaactc accaccaggc
     7861 attcacttca gccactttga tggtgcaaaa ggaagtggcg gagcgagttt gcgcgagtcc
     7921 ggactgcaag gcttacggag tgatgaccct gcgtctgcgg aggattttcg acgcagagat
     7981 tttgcttgat gttccccgaa agtatttcag ccctgccccc aatgtcgatt cagcagtgct
     8041 ttccctcaaa cccagagctg ttccagccgt tatccccgac ctggccaaat atctcaagct
     8101 gatcgaactg gccttcgccc accggcgcaa aaccttgcgg aacaatcttg ccagcctgct
     8161 ggacagagat ggcctcaacc ggcttcagca gcaaagcggc atcgatctca cccgccgcgg
     8221 agaaaccctg agtgaagcgg agtttattgc tctcagttct ttgctttagc tgctgcctgc
     8281 tcgcgtccca ggtcaatctg ctcgatttca gctattcaag cagcaacgct tcccgcaacc
     8341 tggaaaacaa tctccgttgc cagcttgccc ctttcaaagc ctctctgatg cagataaacg
     8401 cccaatcctt cggcgaacag cggctgaatt tctcccaacg cagccgtcgg tcccttctga
     8461 atctggacct ggctttcgac cgcggacttt ttagacacaa cgttcaatcc ggctacgaat
     8521 acctttacga ttccagcgaa ctggatgagg atttccagcc ttaccgcaat aaaacaggat
     8581 ttttgggtta ttctttggaa atccaacctg cggacagcct gctcttcgaa gctggagtgc
     8641 aaggcctgat ccgctctgag gaagaccgct atcttctgga ttccaaaatc aacagccaag
     8701 gctatctggt gcatggcagg gcaaccgcct caaccgggct ctggaactcc cgcgccggac
     8761 taaacgccgc tttcgagcgc aagacaatgg attgggaatt ttatcagacg ggttcccttt
     8821 ccgcctggct gaaccaccag agccaaagcc tgctcttcaa caacaatttc agcctctcgc
     8881 ggcggtatga tgacatttat gtgctgctgc cggacgtcgg aggcaggggt cattatgacc
     8941 tcgacgacag gcagaaacgc agttcgctgg tttattctgg cagcctcgag tatgagcctt
     9001 gggagatcct gcgtctcagc ctgctggaga atttcagcag gaaaatcacc gatctggccc
     9061 aaaacactat gcgcaacaac actgaccttg agaaccaagc ttcgctctcg cttgagctga
     9121 aaccctggtc acgccttggc tggacatcca ctttcagcca cggatacggc cttaagacct
     9181 tcgcccaggc ccaaaccagc cgccagcttg aaaaccgctt cctttcctca cgcctgaatt
     9241 gggaatacac agacggcgac actctgtctg cagcttttgc tgtggacttg cagcgcagca
     9301 cctttccgga cgaccagaac cgctgggaca acgatctgcg ccatatcagg ctcaacttta
     9361 ccaacgccca ttactggaaa gaccgcctca aaatcagcaa cggctttttc tggaatataa
     9421 cagacgacgt atatctggac ggcctgctct cctgcaacaa caaacagacc tccagctacg
     9481 tttacaaccc ctcctgcgct ttgctgatcg gcgaccggct gcttttcaac cagaactacc
     9541 tggtccgggc cgattatacc agttacatgt acgatcccac ggacaaagcg ttttatcgcc
     9601 agttggaaat ggaatacaaa ctggtgttcg acagctttcc cttcgccgcc cgctcccaag
     9661 accagcgctg gctgctgctg ccttaccgca ccaacgatgg aaacgccctg ctggctgacc
     9721 tcagttttgg gttcgagcgc aacgaatacg ccgattacaa cggacaggaa tatgtgatcg
     9781 gatttaaaaa cacccgctac tcggcagctt tcacactcaa acacgacatc caggacctct
     9841 actacatcat ccaaccccga tacacttggg gaacctggat agaatacaat cttttgctgg
     9901 ggctggcttg gaaattcaca gactcctcgc tgttggaatt ttcactaaac cccgtgggtg
     9961 atagtctttc caaccttgac tggcgcagtt ccgtgagctt gggagcgcgt ttctgatgag
    10021 tgcagaacac tcagcctgga tgaagcttgc ccttgaggaa gcaaacctgg cccgctctga
    10081 agatgagatc ccggttgggg cggtattggt gaaaggcggt gagctgatcg ctgctgaaca
    10141 caaccgcacc cgccaattga aagaccccct tgcccacgcc gagaaattgc tcattgacaa
    10201 aatcctcgca tccaaaatca agtacctgca ggattacacc ctgtatgtaa ctttggaacc
    10261 ctgtttgatg tgtgccggaa tgatgatctg gagccggctg ggcactttgg tgtatggcgc
    10321 ttcagacccg aaagccggag ctgtgggttc catctacaac gtgctggcgg acaaaagctt
    10381 caaccatcat ccccgcgtcc tccggggagt gctggaagct gattgcgcgg ccttgctcag
    10441 ggattttttc caaagccgaa gacaatgaag tggagagttg ccggagcggt tgaacgggac
    10501 ggtctcgaaa accgttgtat ccttacgggt acccagggtt cgaatccctg actctccgcc
    10561 attttgatat gaaccagaca gatactcaac ttgtttgtga ctccggttgt ccgctttgcg
    10621 gaggcggcac cctgcgcgag gaattgcttc tttataacat ccccactgaa tcctatcatg
    10681 ctttgcaagt tgaatttgcc tctctgaccg gaaagaaact cagtcccgat agtgatgtca
    10741 gcatcctctc cggcggccaa aaagtcctcc tgatgtgtct ctgcgctcta ttctcccccg
    10801 cccggaaaat tctgttcatc aacctatggc actcgctgga tgctgtaaac cgagctagaa
    10861 ctaaagagct catcacgctc
//
LOCUS       DAOOWR010000010        10565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6282_length_10565_cov_4.331208, whole genome shotgun sequence.
ACCESSION   DAOOWR010000010 DAOOWR010000000
VERSION     DAOOWR010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6282_length_10565_cov_4.331208"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLB85_00655"
     CDS             <1..182
                     /locus_tag="PLB85_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71864.1"
                     /translation="DAIHFIIGFEIYKENNNIYKTKGWKILDAFEVECDVKYEFNSDN
                     LRLYRPHMILAEGII"
     gene            complement(527..1072)
                     /locus_tag="PLB85_00660"
     CDS             complement(527..1072)
                     /locus_tag="PLB85_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71865.1"
                     /translation="MDESLCVKGLEKQKLNHALEWLKARQGKLLGARLALDAFYNREP
                     ENDGFRAPLSNADVDWHGYSHGAIRELLVPMADRKTLYQCRERGRKLIKAAAAHLELG
                     HSEQAEACENELRAIRAYLSEVTAPGGRIRNFPASRVKNYQADMQAWYYLLRKAEEDD
                     PRVHAFLLAHVRPGTPFLWLD"
     gene            1300..1611
                     /locus_tag="PLB85_00665"
     CDS             1300..1611
                     /locus_tag="PLB85_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71866.1"
                     /translation="MTIASSAGGVAALPAFKILRPLRAFFFAPKMPTETVAAAWRKTP
                     EQKALQPCQINYPLYRVKVFAGEFWAFFWGIGDGIFGGVLEGWKVMVRAGIGAENRRQ
                     Q"
     gene            2068..2520
                     /locus_tag="PLB85_00670"
     CDS             2068..2520
                     /locus_tag="PLB85_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71867.1"
                     /translation="MKVKFKYGIATYSGTIDEMVYGSYRDHKLCIGREYVYPRLTVHN
                     DNLGKTGSNLAILWAGASPEYKADLKTYAQRNGNENVPKTQYPPNGYALWVKALHAWQ
                     DKTPSIDISTLTVEDFELGGASVSTVKNAIENGFLKKVANYDDLTEAY"
     gene            2777..2965
                     /locus_tag="PLB85_00675"
     CDS             2777..2965
                     /locus_tag="PLB85_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71868.1"
                     /translation="MNENLLKELARELAAKVNKLVDIPFIKEDDEQAFFELIILLMLE
                     LVFNRLGFKNAVEISPHA"
     gene            3314..3958
                     /locus_tag="PLB85_00680"
     CDS             3314..3958
                     /locus_tag="PLB85_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOU71869.1"
                     /translation="MALALKTTKFVESQIDTFLDVVSDSALLFQLGVEDYLLGRYEQF
                     EERLNLIRENEKKADDLRVAIERYLYERTLIPENRGDVLAILENTDEVMDNIKDTLMQ
                     FSIEMPQIPQEANDLWIQTTKASTAAVEQLTYAVRSFFRDLAQVNNYIHKVYFFEREA
                     DQLGEKLRRLIFAQDMELAQKSHLRWFALHIEQISDYAQAVCDRLAIYAIKRQL"
     gene            3966..5450
                     /locus_tag="PLB85_00685"
     CDS             3966..5450
                     /locus_tag="PLB85_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU71870.1"
                     /translation="MIYIFLISGLFIGWSMGANDTGNIFGAAVSTRMIRFRQAALIAS
                     VFYFLGAVLEGSGPSSTLGRLGSVDALGGAFTVALAAALAIRVIVRLGIPVSVSQTIV
                     GALIGWNYFSGRLTNFGSLLTIASSWITAFVVAAGIAALLFYIVRGWVNNSKRHLLEQ
                     DMIIRYALIFFGAVGSYFLGANTIANAVGVFVPVTPFKDIALGPSLSITGVQQLYIIG
                     ALAVVLGIYTYSHRVMGTVGKDIVQLSPATALVALLTETIVLFLFSSRGLHDLLVSLG
                     LPPIPLVPISSTQVIVGAVIGIGLAKGGRNIRYNVLGKVTLAWIAAPTMAFLFSFVAL
                     FITQNVFELEVRHPLTYSVDKSAVKEIGRRGIDTNKLSFVNLRTFKTEMEIYRELTFE
                     DAYPPEQAREIISISENHPLLVKLELLQKHGLDKRLSPEQLEALSQLEGRRFKRKWNL
                     AQALAQDPAWQLLEKPENRAEETRNARLLADLDLLYKVFYAPGD"
     gene            5574..6464
                     /locus_tag="PLB85_00690"
     CDS             5574..6464
                     /locus_tag="PLB85_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU71871.1"
                     /translation="MTEQNIVLVFSFALGIVLKSLKFLSKDDAPVLLRFMLNVCLPPL
                     MILAIYRATPSANMLLVPLIAMLVVFIIYFISSSIGKKLAMPRPTFGSFLVGTMIMNT
                     AFALPYFKAIGSEELALASLYDIGNTILIFTFSYYNAIKYGDNAHSDRIQIKKFLTLL
                     PLWAMAIAFAIKGFGLHIPPAAITLLEVLSQPIGVLMMVALGLAFAPKLSHLGKALIA
                     VFIRMGLGFAAGFALSLLFGLQGVPRLVVLTCSALPVGFNTLIFADRENMDRSFAATM
                     VSVSTLISLFTTPLLIRLFP"
     gene            6644..7846
                     /locus_tag="PLB85_00695"
     CDS             6644..7846
                     /locus_tag="PLB85_00695"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU71872.1"
                     /translation="MKILVLNCGSSSIKYQFIDIEPRHVLAEGIAERIGEDIALFTYK
                     SEKFTKKKREMVIENHEQGLQLIIDSLLDKVNGVIGDKSEIDAVGHRLVHAGEHYSDA
                     VVITDHVVNVMQECVSLAPLHNPANLKGIEAVKANMPSVPQCGVFDTAFHQSMPPRAF
                     LYPLPLDFYKVHKIRRYGFHGTSHKYVSMQAAEYLNKNIEDLKIITCHVGNGASITAI
                     DGGKSIDTSMGLTPLEGLMMGTRCGDIDPALPIHMQKNMGMGVDEVNVILNKRSGMLG
                     LSQISNDMREIEDQMAIHKNPAAIQAFDVYCYRIKKYIGSYFAAMNGLDLLVFTGGVG
                     ENMSVMREQVCQDMDALGIRLDLEANANAGSDIALLSAPESRVTVLKVPTDEELMIAL
                     ETQRLLSR"
     gene            7892..8155
                     /locus_tag="PLB85_00700"
     CDS             7892..8155
                     /locus_tag="PLB85_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOU71873.1"
                     /translation="MRIDQLLNKLCLTKTRNIAKTACDKGLVSLNGKTAKASAEVREG
                     DLLVFRLYGFEHEIRIDKVPSGNVAKKDATDYYTLLGRKELPN"
     gene            8221..9111
                     /locus_tag="PLB85_00705"
     CDS             8221..9111
                     /locus_tag="PLB85_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71874.1"
                     /translation="MAAFTENFVIHSVDGGFPGGRSFVDELDSRIGKLQMELGVYPEG
                     KAEIFIVPDRASYQALARGRGAIVEFSEAFYSGRERRIYARSADQVPANYSGLILHEY
                     THWLLDELMIGAPLWLHEGLATEYGHQLGLDRYYHYVRERFWGNRMDLFRLAYNYPKQ
                     REDWEMYYLTSYFAVRYMREKDPKAWRAFWNIVAAHNRRGERVRFAEAFGRAYNSNLH
                     SFSLDFAEASRRKAWIYLITGFSSLIFALLPFMVIIATLRQRRKMKALPDLELPAEIE
                     VTVPETDPPEASGQDEQPEP"
     gene            9131..10156
                     /locus_tag="PLB85_00710"
     CDS             9131..10156
                     /locus_tag="PLB85_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOU71875.1"
                     /translation="MYPISFGSDNHSGVHPKVWEALHKADLGYCPAYGDDPLTEKVLK
                     KLEWLFGGGCEAAFLITGTGANVVALQNYLRSYNAVFCANTAHINVDECGAVQKFTQA
                     RLKVIDTRDGKLTPELIHPHLLTNRDQHHSQGRVVSITQSTEYGTLYSLAELKALADF
                     SHEHGMFLHIDGARLANAAAALECSLKEMTKDVGADTVSLGGAKNGLLFGEALISFTP
                     EQTADLRFYRKQGNQLFSKMRYIAAQFDAYLEDELWRVNALHANKMAALLASRLRDIP
                     QVRITQPVTVNSLYVILPPDITPTLQEKYRFYMWNEARNEVRLMCSFNTTEEHVREFV
                     ADLKSML"
     gene            complement(10247..>10565)
                     /locus_tag="PLB85_00715"
     CDS             complement(10247..>10565)
                     /locus_tag="PLB85_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71876.1"
                     /translation="FIPLTFLSSMLTAFAVRQDTSASTTIAPVTQNPRHSGPDPESIA
                     YPFMDPRSMSPHGFGDVRDDVALEAGVEQGTSIDGSSNNPCSPSSQTRWTPGSTNIYG
                     SNE"
BASE COUNT         2322 a         3132 c         2913 g         2198 t
ORIGIN      
        1 acgatgctat tcacttcatc ataggctttg aaatatataa ggagaataat aatatctaca
       61 agaccaaggg gtggaaaata cttgatgcgt ttgaagttga atgtgatgtg aaatatgagt
      121 ttaattctga caatttaaga ttgtaccgac cccatatgat tctagcggag ggaattatat
      181 gacgccagca cgacgaattg attagaggag catcagcagg ctggaaggtt aacgtccttc
      241 cgatacatcc tgccgttgtt ctgcaatttt cctgccgtcc atgcttactg cgttcgctgt
      301 acgccaggat gccatcccca caaagaaaaa gcggacgggg tttcaagccg tccgctaatt
      361 cctttcaggg ccgctgacaa tcatccgcgg ccttgtttcc gtgccagctt caggcccgcg
      421 gcgcgggttt tttcacggtt ccgttccctt tgccggcgtt ggttttcctt tccggcgccc
      481 catcatttcc gtcgctttct cccttcgccc catccccggc aggggttcaa tccagccaga
      541 ggaagggggt tccggggcgg acgtgggcca gcaggaaagc gtggacccgg gggtcgtctt
      601 cctccgcctt gcgcagcagg taataccagg cctgcatgtc cgcctggtag tttttcacgc
      661 ggctggcggg gaagttcctt atcctgcctc cgggggccgt gacctcggaa aggtaggcgc
      721 ggatggcccg cagctcgttt tcgcaggctt cggcctgttc cgagtggcca agctcgagat
      781 gggcggctgc ggctttgatg agcttcctgc cccgctcgcg gcattggtaa agggttttcc
      841 tgtccgccat cgggaccagc aattcgcgga tggcgccgtg gctgtagccg tgccaatcca
      901 cgtccgcgtt tgaaagggga gcccggaatc cgtcgttttc cggttcgcgg ttgtaaaatg
      961 cgtccagcgc gagccgggcg cccagcagtt tgccctgcct ggcttttagc cattccaggg
     1021 cgtggttcag tttttgtttc tccagacctt ttacacagag actctcatcc atcgcccctc
     1081 cccggggtgt ggcggttgga agcggcggaa gcgccggggt tggacgcgcc ggttaaactg
     1141 gcgtccggct taaacacttg agataatgcc ggtttcagca ttattcctcc ttgccggaag
     1201 ctccgcgaag ggagggagtg gaaagttatc cacatccatc cacatttcac acttgacaga
     1261 aacccgacac cgccatataa aggcaaaagc tactgcccta tgacgatagc aagttctgcc
     1321 ggtggtgtgg cagcactgcc ggcttttaag attttgcgcc ccctgagggc tttctttttt
     1381 gccccgaaaa tgccaaccga aaccgtggca gcggcttggc ggaaaacgcc ggagcaaaaa
     1441 gcattgcaac cgtgccaaat taattacccc ttatatcgtg tcaaggtttt tgcaggggaa
     1501 ttttgggctt ttttttgggg gatcggcgat gggatttttg ggggtgtttt ggaggggtgg
     1561 aaagtgatgg tgagagctgg catcggagcg gagaatcgac ggcagcaatg agtggttctt
     1621 tccatttctt cctgtcgtcc atgctccctg cgttcgctgg acgccaggct ccgggatcca
     1681 tgagtgggga tatgtactgg attccgggtc aagcccggaa tgacgtgtaa gacattggcc
     1741 ggaagagccg aacatacgtc atcccggacg tcaccgagcc cgcgaggtga cattgatccg
     1801 ggatccatga aggggatatg cactggattc cgggtcaagc ccggaatgac gtgggatgga
     1861 ggctgggatt gagattgttt ctggacgttg gtttgggagt ttcggcgctt cggcgagcgc
     1921 cgctacctgc agtctgtgta aggctttttc cccggaaacg caaaatattt ccgcgaaacc
     1981 ccatttttct catacgcacg tttatagagg gatgtgtttc cgttcgcgcg gaaaggaaaa
     2041 tcccaataaa acaaggagga cacagccatg aaggtgaaat tcaaatacgg catcgcgacc
     2101 tacagcggca ccatcgacga aatggtctat ggcagctacc gggaccacaa gctctgcatc
     2161 gggcgtgaat acgtgtatcc caggctcacc gtccacaacg acaacctggg caaaaccggc
     2221 agcaacctgg ccatcctttg ggccggggcc tcgcccgagt ataaggcgga ccttaaaacc
     2281 tacgcccagc gcaacggcaa cgaaaacgtg cccaaaacgc agtatccgcc caacggctac
     2341 gcgctctggg tgaaagcgct ccacgcctgg caggacaaga cgcccagcat cgacatctcc
     2401 accctcaccg tggaagactt cgaactcggc ggcgccagcg tcagcaccgt gaaaaacgcc
     2461 atcgaaaacg gcttcctgaa aaaggtcgcc aactacgacg acctcaccga agcctactga
     2521 cccggagggt aagagggggt cggaaggccc cctcctttta ccgtccacga attgaaggga
     2581 acgcggatga caggatctac gggataaaaa gagggatggg ctggcgtaga acgaagcatc
     2641 gacgacaaca gcatcaattc ctgtagtcca tgctcgcagg ctcgctggac tccagggatc
     2701 gggagtcccc ttccaccttt ccacgaactc cgctttccct ctgtgactct gtggtaaaaa
     2761 aaacaaggag agacaaatga acgagaacct gctgaaagaa ctcgcccggg aactggccgc
     2821 caaagtgaac aagctggtgg acatcccctt catcaaagaa gatgacgaac aggcgttctt
     2881 tgaactgatc atcctgctca tgctggaact ggtcttcaac cgcctgggtt tcaagaatgc
     2941 tgtcgaaatc tcgccgcatg cgtaagacgc aaactgcgca agaggcgcaa ggaatgtaat
     3001 aagtgatgga ttccgggtca agcccggaat gacgcgggtg tggggtgaca ggagctattg
     3061 ctgtcgtcca tgctcaccgc gttcgctgga cgccagggat ggtgaagtga cagtgttttc
     3121 tggattccgg gtcaagcccg gaatgacgtg ggtttggggc tgggtggggg tttatgggct
     3181 attcagcccg gactggattc cgggtcaggc ccggaatgac gcataagggt ccgcgctttt
     3241 ccgcttgaca gatttataag gttgaaaata aactgaaagg ttacattgac tataacagag
     3301 aacaggagtt gacatggcac tggcgctgaa aaccaccaag ttcgtggagt cgcagattga
     3361 cacgttcctg gatgtggtaa gcgattccgc gctgctcttc cagcttggcg tggaggatta
     3421 cctgttgggc aggtatgagc agtttgaaga gcggctgaac ctcatccgcg aaaacgaaaa
     3481 aaaggccgac gacctgcgcg tggcgataga gcgttatctt tacgaacgca ccctcatccc
     3541 ggaaaaccgg ggcgacgttc tggccattct ggaaaacacg gacgaggtga tggacaacat
     3601 caaggacacg ctgatgcagt tttccatcga aatgccgcag ataccgcagg aggcgaacga
     3661 cctctggata cagaccacca aagcctcgac agcggccgtg gagcagctca cctatgccgt
     3721 gcgctccttt ttccgcgacc ttgcccaggt gaacaactac atccacaagg tttatttctt
     3781 cgagcgcgag gccgaccagt tgggcgaaaa gctgcgccgc ctcattttcg cccaggatat
     3841 ggaactcgct caaaagtccc atctgcgctg gttcgccctg cacatcgaac aaatctccga
     3901 ttacgcccag gccgtttgcg accgcctggc catctacgcc atcaaaaggc agctctgacc
     3961 ccggcatgat ctacattttc ctgatcagcg gcctgttcat aggttggtcg atgggggcca
     4021 acgacaccgg caacatcttt ggcgccgctg tttccacccg catgatcaga ttccgccagg
     4081 cagccttgat cgcctcggtc ttttactttc tgggagcggt tttggaggga agcggaccct
     4141 ccagcaccct gggccgtttg ggttcggtgg acgccctggg cggcgctttc accgtggcgc
     4201 tggccgccgc gctggccatc cgggtgatag tgcggctggg cattcccgtc tcagtttcac
     4261 agaccatcgt gggcgcgctc atcggctgga actacttttc cgggcgcctc accaacttcg
     4321 gttccctgct caccatcgcc agcagctgga taaccgcctt cgtcgtcgcc gccggaatag
     4381 ccgccctgct cttctacatc gtgcgcggct gggtcaacaa ctcgaagcgc cacctgctgg
     4441 aacaggacat gatcatccgc tacgccctca tcttctttgg cgccgtgggg tcttacttcc
     4501 tgggcgccaa taccatcgcc aacgctgtgg gcgtctttgt tccggtcacg cccttcaagg
     4561 atatcgcgct cggcccctcc ctatccatca ccggcgtgca gcagctttac atcatcggcg
     4621 cactggcagt ggttttgggc atttacacct attcccaccg ggtgatgggc accgtgggca
     4681 aagatatcgt tcagctttcg cccgccacgg cgctggtcgc cctgctcacg gagaccatcg
     4741 tcctctttct cttttcctcc cgcggattgc atgacctgct tgtctcgctg gggcttccgc
     4801 ccattccgct ggtgccgatc tcttccaccc aggtgatcgt gggcgccgtg atcggaatcg
     4861 ggctcgccaa aggcggaaga aacatccgct acaatgtcct cggcaaggtc accctggcct
     4921 ggatcgccgc gccgacgatg gccttccttt tttccttcgt cgccctcttc atcacgcaga
     4981 acgtctttga gctcgaggtc cgccatcccc taacctacag cgtggacaag agcgcggtga
     5041 aagaaatcgg gcgccggggg atcgacacca acaagcttag cttcgtgaac ctgcgcacct
     5101 tcaagaccga gatggaaatc taccgggaac tcactttcga agacgcctac ccgccagaac
     5161 aggccaggga aatcatctcc atcagcgaaa accaccccct gctggtgaag ctggaactgc
     5221 tgcaaaaaca tggcctggac aagcgcctga gccctgaaca actggaagcg ctcagccaac
     5281 tggagggcag gagattcaaa cgcaagtgga acctcgccca agccctggcc caagaccccg
     5341 cctggcaact gctcgagaag ccggaaaacc gggcggagga aacccgcaac gccaggctgc
     5401 tggccgatct ggaccttctc tacaaggtct tttacgcccc cggcgattga agcgggaatc
     5461 cggttttagg aagcgtgtgt ccgcaaccct tatgtctttc aacccttcac ggaggccggc
     5521 cttccccctt ttctccgtgt ctctccttct tccagcccct gcgttaaaaa gccatgactg
     5581 aacaaaacat cgtcctcgtc ttctccttcg ccctcggcat cgttctgaaa agcctgaaat
     5641 tcctttccaa agacgacgcc ccggtgctgc tgcgcttcat gctcaacgtc tgcctgcccc
     5701 cgttgatgat cctcgccatc taccgtgcca cgccttccgc gaacatgctg ctggtcccgc
     5761 tgatcgccat gctggtggtt ttcattatct atttcatttc atcatccatc ggcaaaaaac
     5821 tggccatgcc ccgccccact ttcggctctt tcctggtggg cacgatgatc atgaacacgg
     5881 ccttcgccct gccttatttc aaggctatcg gcagcgagga acttgccctc gcctctcttt
     5941 acgacatcgg caacaccatc ctgatcttca ctttcagcta ctacaacgcc atcaaatacg
     6001 gagacaacgc ccacagcgac cgcatccaga taaagaaatt cctcaccctg ctgccgcttt
     6061 gggccatggc catcgccttt gccatcaagg gattcggact gcacatcccg cctgccgcga
     6121 ttaccttgct ggaggttttg agccagccca tcggagtgct gatgatggtg gctctgggac
     6181 tggctttcgc gcccaagctc tcccatctgg gcaaggcgct gatagccgtg ttcatcagga
     6241 tgggcctggg tttcgccgcc ggattcgcgc tttccctgct cttcgggctg cagggcgtcc
     6301 cgcgcctggt ggtgctcacc tgttccgccc tgccggtggg tttcaacacc cttatctttg
     6361 ccgaccgcga gaacatggac cgctccttcg ccgccaccat ggtctcggtc tcaaccctca
     6421 tatccctctt caccacaccc cttctgatcc gcctctttcc ctgatccggc ctgcctttcg
     6481 cccccacccg cccgagggcc ggggttgtgg ctttttcagc cggacgtgaa tggggcgcgg
     6541 ataaacgtgg acgcgtagcc aaaaagcgcc ggacagccaa atcggttgac agttttgccc
     6601 ctccgcgccg acttggacaa aaaaacaaaa caacgaggaa caaatgaaaa tcttagtgct
     6661 caattgcggc agttcgtcca tcaaatacca gttcatcgac atcgagcccc ggcacgtgct
     6721 ggctgaaggc atcgcggaaa ggatcgggga agacatcgcg ctcttcacct acaaaagcga
     6781 aaaattcacc aagaaaaagc gggaaatggt gatcgagaac cacgaacagg gtctccagct
     6841 cataatcgac tcgctgctgg acaaggttaa cggcgttatc ggcgacaaga gcgaaatcga
     6901 cgccgtggga caccgcctcg tgcatgccgg ggaacactat tcggacgccg tggtgatcac
     6961 ggaccacgtg gtgaacgtga tgcaggaatg cgtctcgctg gcgccgctgc acaaccccgc
     7021 caatctgaaa gggatcgagg ccgtgaaggc caacatgcct tccgttccgc agtgcggggt
     7081 tttcgacacc gccttccatc agtccatgcc tccacgagcc tttctctatc cgcttccgct
     7141 ggacttttac aaggtccaca aaatccgccg ctacggcttc cacggcactt cccacaagta
     7201 tgtgagcatg caggcggctg agtatttgaa caaaaacatc gaggacctga aaatcatcac
     7261 ctgccacgtt ggcaacggcg cctccatcac cgccatagac ggcggcaaaa gcatcgacac
     7321 ctcgatgggc ctaaccccgc tggaaggcct gatgatgggc acccgctgcg gcgacatcga
     7381 ccccgccctg cctatccaca tgcagaaaaa catggggatg ggggtggacg aagtgaacgt
     7441 catcctgaac aagagaagcg gaatgctcgg cctttcccag atttcgaacg acatgcgcga
     7501 aatcgaggac cagatggcta tccacaaaaa ccccgccgcc attcaggcgt ttgacgtata
     7561 ttgctatcgc ataaagaaat acatcggctc atatttcgcc gcgatgaacg gcctggacct
     7621 gctggttttc accggagggg ttggcgaaaa catgtccgtc atgcgcgaac aggtctgcca
     7681 ggatatggat gccctgggca tcaggctgga tcttgaagcc aacgccaacg cgggctccga
     7741 catagcgctg ctgagcgcgc cggaatcccg ggtgacggtg ctgaaggttc ccaccgacga
     7801 agagctgatg atcgccctgg aaacgcagcg gctcttatcc cgttaacaat cagaaccata
     7861 tagaaatacg cctccgaaaa aggggttcgc catgcgtatt gaccaactgc tcaacaagct
     7921 ttgcctcacc aagacccgaa acatcgccaa aacggcctgc gacaagggct tggtgagcct
     7981 gaacggcaaa accgccaaag cctccgccga ggtgagagag ggggatttgc tggtcttcag
     8041 gctctatggc ttcgaacacg aaatcaggat agacaaagtt ccctccggca acgtggccaa
     8101 aaaagacgcc acggactatt atacccttct ggggcgcaag gagttgccga actgaaacgc
     8161 ctgctttggc tgttcgcgct ttgcctgctc gctgcgggat tggcggctca ggaatacaag
     8221 gtggcggcct tcacggaaaa cttcgttatc cacagcgtgg atggcggttt ccccggcggc
     8281 cggagcttcg tggatgaact ggactcgagg ataggaaagc tccagatgga gttgggcgtc
     8341 tatccagaag gcaaggccga aatcttcatc gttcctgacc gcgcctctta ccaggccctg
     8401 gcccggggac gcggcgccat tgtggaattc agcgaggctt tctattccgg ccgggaacgc
     8461 cgcatctatg cccgcagcgc cgaccaggtt cccgccaatt acagcgggct gatcctgcac
     8521 gaatacaccc actggctgct ggacgaactg atgatcgggg cccccctctg gctgcacgaa
     8581 ggcctggcca cggaatacgg gcaccaactg gggctcgacc gctactatca ctatgtgagg
     8641 gaacgctttt ggggcaaccg catggacctc ttcaggctgg cctacaacta tccgaagcag
     8701 cgggaagatt gggaaatgta ctatttaacc tcatatttcg ccgtgcgtta catgcgcgag
     8761 aaagacccga aggcctggcg tgcgttctgg aacatcgtgg ccgcccacaa ccgccgggga
     8821 gaacgggtcc gtttcgcgga agctttcggc cgggcctaca attccaacct ccacagcttc
     8881 agcctggatt tcgcggaagc cagccgccgc aaagcctgga tctacctcat caccggcttt
     8941 tcctcgctaa tcttcgcact gctgcctttt atggtgatca tcgccacgtt gcgccagagg
     9001 aggaaaatga aagccctgcc ggacttggaa ctaccggcgg aaatagaagt tacggttcca
     9061 gaaaccgacc ctcccgaagc atcgggacag gacgaacaac cggagccgtg accgcaatct
     9121 gggagaaaaa atgtatccta taagttttgg aagcgacaac cacagcggag tgcatcccaa
     9181 ggtctgggaa gccttgcaca aagcggacct gggctattgt cccgcctacg gggacgatcc
     9241 cctcaccgaa aaagtgctga aaaagctcga atggctgttc ggaggcggct gcgaggccgc
     9301 tttcctgatc accggaaccg gggcgaacgt ggtggccctg caaaactacc tccgttccta
     9361 caacgcggtt ttttgcgcca acacggccca catcaacgtt gacgagtgcg gagccgtgca
     9421 gaaattcacc caggcgcggc tgaaggtgat cgacacccgg gacggcaagc tgacccccga
     9481 gctcatccat ccgcatctgc tcaccaaccg cgaccagcac cattcccagg gccgggtggt
     9541 ctccatcacc caaagcacgg aatacggcac gctttattct ctggccgaac tcaaggccct
     9601 ggctgacttt tcccacgaac acgggatgtt tctgcacatc gacggcgcac ggctcgccaa
     9661 cgccgccgcc gccctggaat gcagcctgaa agagatgacc aaggacgtcg gggccgacac
     9721 ggtgagcctg ggcggagcga aaaacggcct cctcttcggc gaagcgctga tcagcttcac
     9781 gccggaacag accgcggacc tgcgtttcta ccgcaaacag ggaaaccagc tcttttccaa
     9841 gatgcgctac atcgccgcgc agttcgacgc ctatctggaa gatgagctct ggcgtgtcaa
     9901 cgccctgcac gccaacaaga tggccgcact gctcgcctcc cggctcaggg atattccgca
     9961 agtgcgcata acccagccgg tgacagtgaa ttccctctac gtgatactcc cgcccgacat
    10021 cacccccacc ctccaggaaa aataccgctt ctacatgtgg aacgaagccc gcaacgaggt
    10081 gcggctgatg tgcagtttca acaccacgga agaacatgtc cgggagttcg tggcagacct
    10141 gaaatcaatg ctttgaacgt aactttgtct tgagaaaaag ccgggctggg atttggcctg
    10201 gcacagcgaa cgtagtgagc atgaatggca ggaagcatct gaaaaatcat tcattgctgc
    10261 cgtagatgtt ggtggagcct ggagtccagc gagtttgcga gcttggacta cagggattat
    10321 tgctgctgcc gtcgatgctc gttccttgct cgacgccagc ctccagggct acgtcatccc
    10381 ggacgtcacc gaacccgtga ggtgacattg atctgggatc catgaaagga tatgcaatgg
    10441 attccgggtc aggcccggaa tgacgtgggt tttgggtgac aggagctatt gtcgtcgatg
    10501 ctgatgtatc ctggcgtaca gcgaacgcag tgagcatgga cgacaggaag gtcaacggaa
    10561 tgaat
//
LOCUS       DAOOWR010000011        10293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6499_length_10293_cov_3.411115, whole genome shotgun sequence.
ACCESSION   DAOOWR010000011 DAOOWR010000000
VERSION     DAOOWR010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_10293_cov_3.411115"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..313
                     /gene="trxA"
                     /locus_tag="PLB85_00720"
     CDS             2..313
                     /gene="trxA"
                     /locus_tag="PLB85_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU71877.1"
                     /translation="MEITSQNFEAEVMQSELPVLVDFWAPWCGPCKALGPTVEKIAAE
                     TEGKVKVAKCNIDSAPDIATRLSIMSIPTLLVFHKGEVAAQLIGLVQKDKIMDKLRPY
                     L"
     gene            complement(537..1679)
                     /locus_tag="PLB85_00725"
     CDS             complement(537..1679)
                     /locus_tag="PLB85_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71878.1"
                     /translation="MKRLPLLLLIPLLLLFACEHGTQPEAHGEMPVRINLEPALAHGL
                     NVTQVSVTITKGGFSHQMNLPIVGSMAEGTFTDLEMGTYAIDVAVYNDTLLIATGSGT
                     GIVKPGETTTVHITLHFVPGGLEVTINWGLPYEESRRVLLVGNSHTYFNGGVDAHLQA
                     LVNAAHPEWRVVVDARTGGGYTLENHCNDPNTLTAIATGDWDLVVLQEQSSRPQTDPS
                     LFYQYAAQLNTAINQAGALTGFYLTWAWRNNPEMYEPIRDAYYYIGAYLDALVLPAGV
                     AFHNAHQDTLSFSLYASDNYHPSIYGTYLVACTMLAGIWNINPVGNSYRPPEISPAHA
                     LLLQTIAWDTVQGETHKAGQRCPLPPLPKPLEVLPSHIIPRDEVAA"
     gene            2273..2614
                     /locus_tag="PLB85_00730"
     CDS             2273..2614
                     /locus_tag="PLB85_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71879.1"
                     /translation="MNKKRFEIVEELDPANVSGVPDVHKKHYEELGYRPYRMGDGKVK
                     WLTEGEKAYNKTRGMPDPKRRSKKKFHASDGRLRRKRRDKKTLWTFISEYWVFLVMAA
                     ITVIVLLICLR"
     gene            2649..3422
                     /gene="surE"
                     /locus_tag="PLB85_00735"
     CDS             2649..3422
                     /gene="surE"
                     /locus_tag="PLB85_00735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU71880.1"
                     /translation="MKIMLCNDDGVFALGIRTLATHLREAGHELIIVAPDRERSAASH
                     SITLRRDLRMKKLQPNEYSVDGTPVDCVVVATQKILKEVPDLVISGINAGQNMGEDVL
                     YSGTVAAALEASLLGCKAIAVSINSYQGQNFDTAAGWMVKLLEMGVDTIIPERGILNI
                     NFPNVVSSEIKGVRLTNTGHRKYYNFIKVIEELEDGFVYRIGGDLPDWEIQKGTDAEA
                     IADNFISITPLGASLTLGDAFPSILEWLETNSLLRIENA"
     gene            3422..4057
                     /locus_tag="PLB85_00740"
     CDS             3422..4057
                     /locus_tag="PLB85_00740"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU71881.1"
                     /translation="MRFEDQRALLAKDLARSGITDERVLAAFAKVPREVYVLPQYRDY
                     AYRNQPLPIELAQTISQPLMIAIMLQYLELRDTDTVLEIGTGSGYQSALLAELAETVC
                     TVERLEALSLKAQGILKEQGYKNIHFRIGDGAQGWQKAYPAFREFNKIVVSAAATEIP
                     PRLKEQLAVDGILVVPVGSGYYQILNKVVRTAEGFTTSEHGGCTFVPLIST"
     gene            4069..4533
                     /locus_tag="PLB85_00745"
     CDS             4069..4533
                     /locus_tag="PLB85_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOU71882.1"
                     /translation="MASKLLLWFKRLFTAADAHNELLRYSLFKDLNSHQRALVAGFLH
                     NREFRAGESVFERGNPLEVIYFVESGEMEVSPLFEGEGSTVLKKNQFIGVLDLFSGKK
                     RLSSVKALTDLKLKALSVDDLQEMLARDPALGVKLLQACCCFLGGYIRSRIH"
     gene            4505..5602
                     /locus_tag="PLB85_00750"
     CDS             4505..5602
                     /locus_tag="PLB85_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU71883.1"
                     /translation="MDTSGAASTNKIMNWNRLIFTIIILAACILAFIFYRPLLMYLLL
                     SLVLAYFLDPVITWMEYKRVPRWLGTLIIYLVILGLLAWLIATYVPVLIREGNQFLDL
                     LGRTDRPLLQTILDLPGFKSLHEFVESLDHSVPQMGLLTRFETFLETAVTKISEFPQL
                     LISNYQSILGTLAMVLMVPIFSFFLLSDKKSIRRGIMSLVPNKYFEISLILLKKVDEN
                     VGNYLRAILLEMLAVGIMSTIALGMLGVPYSVVIGAIAGLTNIIPYIGPWLGGFIAVL
                     VILFSGMPSVNILWACIAMFLVQQVDNYVVYPAIVGKTMKMQPLLVILTVLAGSYFGG
                     VFGMLFSVPLVYMIFSLLTATQKYLKEFRII"
     gene            5615..6793
                     /locus_tag="PLB85_00755"
     CDS             5615..6793
                     /locus_tag="PLB85_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOU71884.1"
                     /translation="MSLIDKCHSFQDAKRAIAAGYYPYFREISSEQDTEVICNGQKML
                     MLGSNSYLGLTTHPKVKEASIAAVKKYGSGCAGSRFLNGTLDIHIQLEEELARLVGKG
                     AALAYPTGYQANLGCISAIVNRDEFMITDKFDHASIIDGCALSLGTMLRYNHNDMAAL
                     ERALKKTDGKNSLVIVDGIFSMEGDIANLPEIVKLCQKYGASLMVDEAHSLGVLGKKG
                     AGAAEHFGLIAETDLIMGTFSKSLASVGGFIAAGEEVIHYLKHKSRALIFSASLPPAS
                     TASVLAALKIMESEPERIERLWEITHYMLREFQAMGYDTGTSCTPVIPLHVGEMKVAF
                     EMWKRLGEEGVFINPVVPPAVPPNSCLIRTSFMATHTNEQLDFALDKFRTIGKQLGVI
                     "
     gene            6847..8577
                     /locus_tag="PLB85_00760"
     CDS             6847..8577
                     /locus_tag="PLB85_00760"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOU71885.1"
                     /translation="MASKSSAEYYGSLKEMSPIRAIAETLMNNDKVRKIDIRQAYEMA
                     KKQPGITVTDLPVYPEFVRLHNLPADAKVLDDCHGNIIGRTAKARRFYHRLDASKKNK
                     LEGDFREAVWQMQHYPLVKAEAILGMDKDLMLKATFITTESDVANLYNWLLNFAPYEQ
                     LKEQYEASPKLPIQDIILIAFNEWTCDDPFYNNVGAPQLALVDEKHNVIVNLGMRYFG
                     ERKKGTLTLAWTSGIRIGMAACHGGIKELDFSTCDDPKFHALGKRSIAFYGLSGTGKS
                     SHTNSHDNAGTMPQGVSKVVLHDDAFQIDLENKICRVWEPTLFDKTDSRPPDHPDWKY
                     ALAVMNHAVVELDGKRIPIGQDTRNQNGRALLDRSLLGNYVNRCAFPKALVWLMKDSA
                     LPPILKLADKHLAIAMGAALMTQRNRAENVTEDELKKLVFEPFANPFRVYELYRDVEA
                     FIQVADNGADFYCFNSRGYWKESDEVLEAIPLKTSLTLQTAILLDQLQWEPWTALPGA
                     MIPTRESIEKILPGYYDLYDPAKRGNLDKYQELLKDRFQQRRDFLMGSDLQEKPELQK
                     LVVDALNLKI"
     gene            complement(8848..9894)
                     /locus_tag="PLB85_00765"
     CDS             complement(8848..9894)
                     /locus_tag="PLB85_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOU71886.1"
                     /translation="MENRNTEQPGAIGLRPMPEDLREALKELYSWDKPPEYFAGGHAW
                     SDGTIYSYSRQGEDRLLKVMPAGSQRSLASVAERQAWQEWLNQNGVDTLPPLHSEAGK
                     LAEAVICGGQEYIVYAWRMVSGKQVQLGDPRDCSDFYRRWGEMLGKMHRLAKSWPQWQ
                     HSACIDASGSPLISRQREWEVFRRRFQDDEVRAAWSVMKTELDALPVTRDNHGFLHND
                     PHPGNILAEDNRLILIDFDVANYLWFMVEIAICVYSEYSRVNFHSPWARRNAELDAIF
                     ITPFMEAYESENTLPAEEYGRVELFLNYRRFLMFACFYEQIREGSPEYLARMKQDIIH
                     ARNYLPEDNWFARR"
     gene            10104..>10293
                     /locus_tag="PLB85_00770"
     CDS             10104..>10293
                     /locus_tag="PLB85_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71887.1"
                     /translation="MKRFALLLLWLGCAVGMLCAQIPLYEISVPPVFVTQSYYDYVMG
                     GYSDLPVAEFAPDQGSGRV"
BASE COUNT         2382 a         2816 c         2759 g         2336 t
ORIGIN      
        1 gctggagatc acaagccaaa atttcgaagc ggaagtgatg cagtccgaac tgcccgttct
       61 ggttgatttc tgggcgccct ggtgcggtcc ctgcaaggct ttggggccaa ccgtggaaaa
      121 gatcgccgcc gaaaccgagg gcaaggtcaa ggttgccaaa tgcaacatcg acagcgcccc
      181 ggatatcgcc acccgtctat ccattatgtc cattcccacc cttttggtct tccacaaggg
      241 tgaggttgcc gcgcagctta tcgggctggt gcagaaagac aagatcatgg acaaactccg
      301 tccttatctc taagccactc ccgcaaataa tcccgcgccc cgctggacca gttccccggg
      361 gcgttttttt acccgactac agacggaaag gcatgatatc cctgtttttt tggagagctg
      421 gttatggtga gaaccgcaga tacggatggg agatataagt cccgctgtag tcgatgctcg
      481 ttccttgctc gactccagta aggtattgcc ggtattacag tagtctgaaa accttatcag
      541 gccgccactt cgtcacgcgg gatgatatgg gacggcaaaa cctcaagcgg ttttggcaaa
      601 ggcggcaatg gacatctctg tcccgctttg tgggtttctc cctgcacggt gtcccaggct
      661 atggtttgca gcagcagagc atgcgcgggg ctgatttccg gagggcggta gctattgccg
      721 acgggattga tgttccagat tccggccagc atggtgcagg ccaccaagta ggttccgtag
      781 atgctgggat ggtagttgtc cgaggcatac agactgaagg aaagcgtatc ctggtgggcg
      841 ttgtggaaag ccaccccggc cggcaggacc aacgcgtcca gataggcgcc aatatagtaa
      901 tacgcgtccc gtatgggttc atacatttcc ggattgttac gccaggccca ggtcaagtaa
      961 aaccctgtca gggctccggc ttggtttatg gcagtgttga gttgggcggc gtattggtag
     1021 aagaggctgg gatcggtttg gggccggctg ctttgctcct gaaggaccac caaatcccaa
     1081 tcccctgtgg cgatggcagt tagtgtgttc ggatcgttgc aatggttttc cagggtgtag
     1141 ccgcctccgg tccgggcatc cacaacgaca cgccattccg gatgcgccgc gttgacaagg
     1201 gcctgtaaat gggcgtccac gcctccattg aaataggtat ggctgtttcc aaccagcaga
     1261 acccgccggc tctcttcgta gggcaggccc cagttgatcg tgacctccag gccgccgggg
     1321 acgaagtgta aagtgatatg gacggtcgtg gtttcgcctg gtttgacaat cccggttccg
     1381 cttcccgtgg cgatcagcag ggtatcgttg taaacggcga catcgatggc gtaagtgccc
     1441 atctcaaggt cggtaaatgt tccttcagcc atgcttccta caattggaag gttcatttga
     1501 tgggaaaaac cgcccttggt gatggtcaca ctcacctgcg tgacgttcag gccatgggcc
     1561 agggccggtt ccagattgat ccgcacgggc atttctccgt gggcttcagg ctgggtgccg
     1621 tgttcacaag cgaatagcaa caacagcgga attagcaaaa gcaaaggtaa tcttttcatc
     1681 taactctcct gttcaggaat ggtcccttcg gttctttgac tgttccgaag accgttttcc
     1741 agcgtgctgt gaagcttgat gttgtcaagt tttttcacgt ccttacattc cccgttttta
     1801 gggtagggta ttattaaagg gttcattttt gtccacaggc ctatgtatag attgatctta
     1861 acccggtcac cggactgaag cattcacgcg gaagcatggt caacatatag ttaatggagc
     1921 cataaatgtt tagccaccgt agtatcctgg ctgccaggtc ctgagcgctc ttggtcatcc
     1981 ctctctaacg cctcccgcct aatcctcgca tcaaatgcgg ttatcttacg ggaggcacat
     2041 cagggacgag gtaaagggcc ttaacggcca gcaggtaacg cttcatccgt gtggctttat
     2101 accccacgaa tatgctttgc tcgctcgcga aaaccccgga accgggacga cggcagttcc
     2161 acccaatcca tttgaacatg aaccttaaaa aacgaaagag ggatgcgggt ttggagatag
     2221 tgcttgacag aattatttgg ccagatttcg atagcttttc aaaggatatc gtatgaacaa
     2281 aaaacgcttc gaaatcgtcg aagagctaga tccggcaaat gtctcgggcg ttcctgacgt
     2341 gcataaaaaa cactatgagg aattgggata tagaccttac cggatgggtg acggcaaagt
     2401 gaagtggctt acggaggggg aaaaagctta taataaaacg cgaggaatgc cagatccgaa
     2461 aaggagatcg aaaaaaaagt ttcatgcctc agacggcaga ttgaggagaa agcggcgcga
     2521 caaaaagacg ctttggacct ttatctccga atactgggtg tttctggtta tggctgcgat
     2581 cacagtgatc gtgctgctga tttgtttgcg ctgagccaga aatatgatgt ttactatgga
     2641 gtaatttaat gaagataatg ttatgtaacg atgacggagt tttcgctctg ggcatcagga
     2701 ccttggccac tcatctcagg gaggccgggc atgagctgat tatcgtggcg ccggaccggg
     2761 aacgcagcgc ggcttcgcat tcgataaccc tgcgccggga cttgagaatg aaaaaattgc
     2821 agccaaatga atacagcgtg gacggcaccc cggtggattg cgtcgtggtg gccacccaga
     2881 aaatcctgaa agaagtgccg gacctggtga tctcaggcat caacgccggg cagaacatgg
     2941 gcgaggacgt gctctactcc ggaactgttg ctgccgcctt ggaagccagc ctcttgggct
     3001 gcaaggccat agcagtttcc atcaattcat atcaggggca gaattttgac acagcagccg
     3061 gctggatggt gaagctgctg gaaatgggag tggacacaat cattccagag cgcggtatcc
     3121 tgaacatcaa ttttcccaat gtggtctcct cagaaataaa aggtgtcagg ctaaccaaca
     3181 cgggacaccg caagtattac aatttcatca aggtgatcga agaacttgag gacggctttg
     3241 tttaccgtat cggcggagac ctgcccgatt gggaaatcca gaagggaaca gatgccgagg
     3301 ccatcgccga taacttcatt tctatcactc cgttgggagc cagccttact cttggcgacg
     3361 cttttcccag catcctggaa tggctggaga ccaactccct gctccggatt gagaatgcct
     3421 gatgcggttt gaagaccagc gagcgctttt ggcgaaggac ctggcccgtt ccgggatcac
     3481 ggatgaacgg gtgttggccg ctttcgccaa agtgccccgt gaggtctatg tgttgcctca
     3541 atacagggat tacgcctacc gcaaccaacc tctgcccatc gaactggccc aaaccatatc
     3601 ccagcctttg atgatagcca tcatgctgca gtatctggaa ctcagggata cggacacagt
     3661 gctggaaatc ggcaccggca gcggctatca gagcgccttg ttggccgaac tggcagaaac
     3721 cgtctgcaca gtggagcgtc tggaagccct ctctctgaaa gcccagggca tacttaaaga
     3781 acagggttat aaaaacatcc atttccgcat cggcgacggc gcccaaggtt ggcaaaaggc
     3841 ctatccagcc ttcagggaat tcaacaagat tgtggtaagt gccgctgcta cagagattcc
     3901 cccgcgcctc aaagaacaac tggcagttga cggaattctg gttgtgcccg tggggagtgg
     3961 ttattaccag atcctgaaca aagtggtccg cactgccgaa ggcttcacca ccagcgaaca
     4021 cggcggctgc actttcgtgc cgcttatcag cacatgaagg aggatgattt ggcttcaaaa
     4081 ctattgcttt ggttcaagcg tctgttcact gccgcggatg ctcacaacga actgctgcgc
     4141 tattctcttt tcaaagatct taattcccat cagcgtgctt tggtggccgg ttttctgcac
     4201 aacagggaat ttcgggctgg ggagagtgtt ttcgagcgtg gcaatccgct ggaagtgata
     4261 tatttcgtgg aatcaggcga aatggaggta tcacccctct tcgagggcga agggtcgact
     4321 gtgctgaaaa agaaccaatt catcggtgtg ctggacctgt tttccggcaa aaaacggctc
     4381 agcagcgtca aggctctcac agacctgaag ctaaaagccc tctccgtgga tgacctgcag
     4441 gaaatgctgg cccgtgaccc tgcccttgga gtaaaactcc ttcaagcctg ctgctgcttc
     4501 ctgggtggat acatccggag ccgcatccac taacaagatc atgaactgga accggctcat
     4561 cttcaccatc atcatcctgg cagcctgcat actggccttc attttttatc ggccgctgct
     4621 gatgtacctt ctgctgtcgc tggtgttggc ctattttctg gatcctgtca ttacctggat
     4681 ggaatataaa cgagtgcccc gatggctggg aacgctgatc atctatttgg tcatcctggg
     4741 cttgctggct tggttgatcg caacctatgt gccagtgctg atccgggaag gcaatcagtt
     4801 cctggacctg cttggccgaa cagacagacc gctgcttcaa acgatcctgg atttgcccgg
     4861 cttcaagtcg cttcacgagt ttgtggaaag cctcgaccat tcggttcccc agatgggttt
     4921 gctcactcgg ttcgagactt ttctggagac cgcagtaacc aagatcagtg aattccccca
     4981 actcctgatt tccaactatc agtccatact tggcactctt gccatggtgc tgatggtgcc
     5041 catattctcg tttttcctgc taagcgacaa aaaatccatc cgccgcggca taatgagcct
     5101 cgtgccaaac aagtatttcg agatttcact gatcctgctg aagaaggtcg atgagaacgt
     5161 gggtaattat ctgcgcgcca tcctgctgga gatgctggcg gttggcatca tgtcaacaat
     5221 tgcgcttggc atgcttgggg taccctactc agtggtgatc ggcgccattg caggcctaac
     5281 caacatcatt ccctacatcg gcccctggct gggaggcttt atcgccgtct tggtcatcct
     5341 gttcagcggc atgccgtcgg tgaacattct ctgggcctgc attgccatgt tcctggtgca
     5401 gcaggttgac aactacgtgg tctatcccgc cattgtggga aaaaccatga aaatgcaacc
     5461 tttgttggtg atcctaacgg tattggccgg cagctatttt ggcggcgtgt tcggcatgct
     5521 gttctccgtg cctttggttt acatgatctt cagcctgctc acggctaccc aaaagtattt
     5581 gaaagaattc cgtattatct gaattgaggt ataaatgagt ctgatcgaca aatgccactc
     5641 cttccaagac gccaaacgcg ctatcgctgc cggctattat ccttatttcc gggaaatatc
     5701 ttcggaacag gataccgagg ttatctgcaa cggccagaaa atgcttatgc tcggttccaa
     5761 cagctatctg gggctcacca ctcatcccaa ggtgaaggaa gcctccatcg ccgcggtgaa
     5821 aaaatatggc agtggctgtg ccggatcgcg cttcctgaac ggcaccctgg acattcacat
     5881 ccagctcgag gaggaactcg cccgcctcgt tggaaaaggc gccgcgctgg cctatccaac
     5941 aggataccaa gccaacctgg gctgcatctc cgcgatagtg aacagagacg agttcatgat
     6001 caccgacaag tttgaccatg cctcgatcat cgacggctgc gcgctctcac tgggcaccat
     6061 gctgcgttac aaccataatg acatggctgc tctggagcga gctctgaaaa agacagacgg
     6121 caaaaacagc ctggtcatcg tggacggaat tttctccatg gaaggcgaca tcgccaatct
     6181 gccggagata gtgaagctct gccagaaata cggagccagc ctgatggtgg acgaagccca
     6241 ttctctgggc gttttgggta aaaagggggc cggcgccgct gagcattttg gcctcatcgc
     6301 cgagacagac ctgatcatgg ggaccttcag taaatctttg gcctccgtgg gcggcttcat
     6361 cgccgcaggc gaagaggtga tccactatct gaagcacaaa tcccgtgccc tgatcttttc
     6421 cgcttcgttg cccccggcct ccacagccag cgtgttggca gcattgaaga tcatggagtc
     6481 cgaaccggag cggatcgaac gcctttggga aatcacacac tacatgctaa gagagttcca
     6541 ggcaatgggt tacgacaccg gaacatcctg cacaccggtg attccgctgc atgtgggcga
     6601 gatgaaggtc gcctttgaaa tgtggaaacg ccttggcgaa gagggcgtct ttatcaatcc
     6661 cgttgtgccg ccggccgttc cgccaaactc ctgtctcatc cgcacatcct ttatggccac
     6721 ccacacgaat gaacaactgg atttcgccct ggacaagttc cgcaccatcg ggaagcaact
     6781 tggcgtaatt tgacaccgcc tgatttgatt ttcaacatat aaaatatagc cataccagga
     6841 ggaaacatgg ccagtaaaag cagtgccgaa tattacggca gcctgaaaga gatgtctccg
     6901 atccgcgcca tcgccgagac gctcatgaac aacgacaaag tgcgcaaaat cgacatccgc
     6961 caagcctatg aaatggcgaa gaaacagccc ggcataaccg ttaccgacct ccccgtctat
     7021 ccggaatttg tgagactgca caatcttccc gctgatgcca aagtgttgga cgactgccac
     7081 ggcaacatca ttggccgcac cgcgaaagcc cgccgttttt accatcgcct tgacgccagc
     7141 aagaaaaaca agctggaagg cgacttccgc gaagcagtat ggcaaatgca gcactacccg
     7201 ctggtgaagg ctgaggcgat cctcggcatg gacaaagacc tgatgctaaa agccaccttc
     7261 atcaccacgg agagcgacgt cgccaacctt tacaactggc tgctgaactt cgcgccttac
     7321 gaacagctca aagaacagta tgaagccagc ccaaaactgc ccatccagga catcatcctc
     7381 atcgccttca acgaatggac ctgcgacgat cctttctaca acaacgtcgg cgctcctcag
     7441 ttggccctcg ttgacgaaaa gcacaacgtg atcgtcaacc tgggcatgcg ctatttcggt
     7501 gaaaggaaaa aaggcaccct caccctggcc tggacttccg gcatccgcat cggcatggcc
     7561 gcttgtcacg gtggcatcaa ggaactggat ttctccacct gcgacgaccc caaattccac
     7621 gccctcggca aacgctccat cgccttctac ggcctctccg gaacgggaaa atcctcccac
     7681 acgaacagcc acgacaacgc cggcaccatg ccccagggcg tttccaaagt tgtgcttcac
     7741 gacgacgcct tccagatcga cctggaaaac aagatctgcc gcgtgtggga acccaccctc
     7801 tttgacaaga cggacagccg cccgccggac catccggact ggaaatatgc cctcgccgtg
     7861 atgaaccacg cggttgtgga actcgacggc aagcggatcc ccatcggtca ggacacccgc
     7921 aaccaaaacg gccgtgccct gctcgaccgc tccctgctgg gcaattacgt gaaccgctgc
     7981 gctttcccca aggctttggt ctggctgatg aaagactccg cgctgcctcc catcctcaaa
     8041 ctggcggaca aacaccttgc catcgccatg ggcgccgcgc tcatgaccca gcgcaaccgg
     8101 gccgagaacg tcaccgagga cgagcttaaa aagctggtct tcgagccttt cgccaatcca
     8161 ttccgcgttt atgagctcta ccgcgacgtc gaagccttta tccaggtggc cgacaacggt
     8221 gcggatttct attgcttcaa ctcccgcggt tactggaaag aatctgacga agtgctggaa
     8281 gccattccgc tcaaaacctc cctcaccctc cagaccgcca tcctgctcga ccaactgcag
     8341 tgggaaccct ggaccgccct gcccggagcg atgattccca cccgcgaaag catcgaaaag
     8401 atcctgcccg gttattacga cctctacgat ccggccaaac gcggcaacct ggacaaatac
     8461 caggaactgc tgaaagaccg cttccagcag cgccgcgatt tcctgatggg cagcgacctc
     8521 caggaaaaac ccgaacttca gaaactggtg gtggatgccc tcaacctgaa aatctgagca
     8581 actcaaccaa tcataatgaa gaagcccggt tatgccgggc ttttttctgg cttaggaaaa
     8641 ggctcatgtt cagatggagt gggcgcggtt gccctcattc cagcttcaaa gtcctttcga
     8701 atgagcgtag cgatgccagt tggccgttaa ggccctttcc tgtgtccctg tcgtgcctcc
     8761 cgcatgatgc ccgcatttga tgcgagtatt gtgcgggaga catgggagtg gaatggaaaa
     8821 gggcgctcag ggtgtagcca ccgggaacta tcggcgggcg aaccagttat cttccggtaa
     8881 atagttccgg gcgtggatga tatcctgttt catccgggcc agatactccg gcgagccttc
     8941 cctgatctgt tcatagaagc aggcgaacat caggaagcgg cggtaattga ggaaaagttc
     9001 cacgcggccg tattcttcag caggcagcgt gttttcgctt tcgtaagcct ccatgaaggg
     9061 ggtgatgaaa atagcgtcca gttcggcatt gcgcctggcc cagggggaat ggaaattcac
     9121 gcgcgaatac tcactgtaaa cgcagatggc gatttccacc atgaaccaga gatagttcgc
     9181 cacgtcgaaa tcgatcagga tgaggcggtt gtcctctgcc aggatattgc cgggatgggg
     9241 gtcattgtgt aaaaagccgt gattgtcgcg ggtgactgga agggcatcca gttcggtttt
     9301 catcacagac caggcagcac ggacctcatc gtcttgaaac cggcggcgga aaacctccca
     9361 ctctcgctgc cggctgataa gagggctgcc gctggcgtct atgcaggcgg aatgttgcca
     9421 ctggggccag cttttggcca gacggtgcat ttttcccagc atctcccccc agcgtctgta
     9481 aaaatctgag caatcgcgcg gatcgcctaa ctggacttgc tttccggaca ccatcctcca
     9541 ggcgtaaaca atatattcct gaccgccgca gatgacagcc tccgccagct tgcccgcttc
     9601 cgaatgcaga gggggcaagg tgtccacacc gttctggttt agccattcct gccaggcttg
     9661 acgctccgcc acgctggcca gggagcgttg agaacctgct ggcatcacct tgagcaggcg
     9721 gtcttccccc tggcgggaat agctgtagat agtgccgtct gaccaggcat ggcctccggc
     9781 gaagtattcc ggtggcttat cccaagagta aagctctttc aaagcttcac gcaaatcctc
     9841 cggcatcggc ctgaggccta tcgcgccggg ttgttcagtg tttctgtttt ccatatcagt
     9901 tcactttcaa agctatgggc caaaggattt accggaccgc ggtggcgtca aggaaaagtc
     9961 ggtggggcag tgctgggcga tgccgaacct attaagcaat gatgcgaata ttttacttga
    10021 caatgggggg ggtaaagaga atcgtttcca tggggtggtt tggattttta tggaatatat
    10081 tggccgcccc gacgaggttc aacatgaaac gttttgcttt gcttttgctt tggctcggtt
    10141 gcgctgtggg aatgctttgc gcccaaatcc ccttgtatga gatctccgtt cctccggttt
    10201 tcgttaccca aagctattac gactatgtga tgggcggcta cagcgacctg cccgtggccg
    10261 agtttgcacc ggaccagggc agcggccggg tga
//
LOCUS       DAOOWR010000012        10208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6568_length_10208_cov_3.143110, whole genome shotgun sequence.
ACCESSION   DAOOWR010000012 DAOOWR010000000
VERSION     DAOOWR010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6568_length_10208_cov_3.143110"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLB85_00775"
     CDS             <1..560
                     /locus_tag="PLB85_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71888.1"
                     /translation="EHPKSRYGDLYKLLHQAYYGPTHIVPDPVAIATDIREEMAAIQT
                     QNCGPSQDIGCGKGFVRLNLVALTVFRDISGSKTSNLLRDQLLRDAMQKVKPEKVKLL
                     TQSILASRLEGVFTLQDWQSIWKQVQPLVCEFIFPTFTERALIEQCLETGLIPPHSDD
                     YRYLYKPHYRVIHHSHLAKFKTHKG"
     gene            564..1997
                     /gene="gltX"
                     /locus_tag="PLB85_00780"
     CDS             564..1997
                     /gene="gltX"
                     /locus_tag="PLB85_00780"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU71889.1"
                     /translation="MNPVRVRFAPSPTGFLHIGGLRTALYDYLFARHSGGTFILRIED
                     TDRNRLVEGAVENLIASLGRLGIEIDEGPGIGGDFGPYVQSERLELYHREAQRLLDSG
                     HAYRCFCTPETLAQMREEQQRKGEFVKYDRRCLSLSEEEVKARLANNEPHVVRLKMPD
                     NRVFSFNDIIRGDVEMDAAQSDDQVLVKSDGFPTYHLAAVVDDHYMQISHVIRGEEWL
                     SSTPKHIWLYECLGWKAPFWVHLPLILNPDRSKLSKRMNDVSVESYLQKGYLKEALLN
                     FVALLGWHGADDRETYSLKELCEEFTLERVSKSGAVFDLTKLDWMNGWYLRNLPLEEV
                     VESSLPWFEEEKLPLPDSLTLSRIVAAARERCTLLPEIAQYAKMFLCPAELGPDDGAF
                     LQSEDSRRVLRWFQSQLPPDGEIDAASLDAIVKSGMAELGLKGKAYYTPLRLALIHQQ
                     HGPDLPTTFAILGLEEVHRRLAAAIRD"
     gene            2386..3030
                     /locus_tag="PLB85_00785"
     CDS             2386..3030
                     /locus_tag="PLB85_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU71890.1"
                     /translation="MMNPVPDKKERILTAATELFSRWGYDKTSLDEVAARARIAKATI
                     YYYFPSKEQLFIAAFSAKAEELFAKLDEEIASASSFEDKLSCFLRLPMIYVFKHMPIL
                     IEAIHQMPTDYLGNLDEYRNNYRNQMNALLAGILDNARSAGIINEWVDSQRFSELISD
                     WFMLGDSWIDIQEKDKIIRRIERDHELIVQLLLYGIIKPSVKDSARRNSDGAKP"
     gene            3027..4403
                     /locus_tag="PLB85_00790"
     CDS             3027..4403
                     /locus_tag="PLB85_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU71891.1"
                     /translation="MKRHLIFMLLLAAVCLNGITLEESLELARQRNSTLLMAREEINR
                     AEQSNLELKGGFFPKLTLAGAYSLQRTYLPSSAMSDPIDLTQLLNPYTASDNDYMLAA
                     SVSGIANSLLPSSPMDEGSLAASLKLEQVIFAGGRLHNGYKATQRYRDISRLNLKVKE
                     QDVALQTIQMFYGYLLTTRLVQVQEEALATLRRHVAQVELFNQEGFVAEFDVLRARLE
                     EAKLEPQLMKAKNDRDLALAAFRKQIGDEQETTVPEDEFALPERIDLSLEQALAQGLA
                     NRGELEMAGLATELKELMWKAEKGIYMPTLGLQASASLFTAADEFAIEADDFGTNYSV
                     GIGISIPLFDGMSNRAKVKAAHHDYLLSRIQQRDATALIRLEITQNYNNLRHAEENYR
                     VQQENMRLAEQGLELARVRYENQVGIQLEVFDAQTTLSAIKLQYYQAIYEVISATRML
                     QKSLGTTL"
     gene            4415..5440
                     /locus_tag="PLB85_00795"
     CDS             4415..5440
                     /locus_tag="PLB85_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU71892.1"
                     /translation="MKQTIITVICCLALLLAACGGKDQAGKSMEQLHKEQGIPVRVKT
                     VAAENFSKDLSYNATLGGSSESYGLSMLAEVVAGVHAKVGDRVSAGQVIVTFPKTTPS
                     AQFEQASTGYNAARQAYERVKNLQAAGAVSQQDLDNAETQFKLAQANLEASRQLITVT
                     SPISGVLTSLAVNPGEMSHPGQILFAVSGTGGYKAKFTVPDKVARSIKIGTSATATIR
                     DMELTGRVSKISLALDPYTQAVPVEVSLPAAGQRISYGATAEIRLHTQSQADAIVVNR
                     EHIVTENGQKYVWVDEKGKAAKRPIETGMDNQLQFEVVSGLQPGDQLITEGINLLSEN
                     ARIRVVK"
     gene            5451..8516
                     /locus_tag="PLB85_00800"
     CDS             5451..8516
                     /locus_tag="PLB85_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU71893.1"
                     /translation="MILSDLSVKRPVLISMVVLVFVVFGVLAWFALPLNLMPEVNLPF
                     IVIQTVYPGAGPSTVETQVTKKIEDALATVSLVDYTQSYSLDNASIIMVAFKMKKNVD
                     IANQEVKDKIDAIQRDLPDAIEKPIVMKLNMNAVPFMDIILSGNMDGRELYELADTKL
                     KDRFGQIAGVGEVSITGGAKRQIEVTLKDRIVHENRISMPQLLQLLAAQNIDMPAGNF
                     KRGSQEYSVRLKGQYTSLDKIADADIPTAYGMKKLRDLAEITDGAEEVRSRAIYHNVE
                     AKSIHDNIVRISLTNAPDGNVVNIAKEVRQMMDEIEADLPAGASLDIVRDDSDFIRGT
                     VDDTFSNILLGILFTALVLLIFLHDWRSTLIVGLSMPISIIATFMFMQIAGYSFNLLT
                     LIGISCSVGVLVSNSVVVIENIFRYKELGKNRRDAAMQGTSEIALAVMASTLTNIVVF
                     IPIASMTTLVGQFFREFALTVTFATIFSLVTSFTVTPMLASRILKEGSTQNRFGIKFD
                     KLFDRFSAWYKRFLGRVLSSKKRSRGIIIITVAVLVVSFALIPVVGLEFMPEMDQGAM
                     SLTVELPQGSNLDQTASTLQTIHERLGKHPEISHVITNLGSAGYVDTGTNLASSSIKL
                     VEPNRRKLNSKQLADRVTRELADIPNARIKVSAVSSGFGEDTGLEFYLQGQDNARLEQ
                     LKTTITNRIKDAPGLINLDTSTRGGRSELTITPKRAEMAAVGATVYDLALALRAAVEG
                     MVSTQYHEGDNQYDIKLSLEDAAVDSPDKIGNLSVVVNYQPYLLSQLADVSFGEGTNR
                     ITHRDRSKAVVFTAGVAEGATMGDVISAVKERLEDVELPRGYKIVWGGSAEMLGEMVK
                     DMLKTLILAVLLTYMLLAAMLESLVQPLFILGTVPLAVIGVIFSLLISGQTFNLVSLM
                     SIIMLVGIVVNNAILLLDYANLRRKQGISAYEALMEAGETKLKPIIMSTLAIVIGMLP
                     MALGVGPAGKELRMPMGIVMMGGLLVSAFLTLVIIPAFYYLTTKQAKSD"
     gene            8834..9304
                     /locus_tag="PLB85_00805"
     CDS             8834..9304
                     /locus_tag="PLB85_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71894.1"
                     /translation="MKKALLLLVLLIGAASVFALPLNVGQSQFNIGTGFSTWGMPIYM
                     GVDYGVTPDITLGGEATLRFHNHGSWVGIYGNGNYHFVNLLNMPSNTDFYAGLCLGFD
                     VWLPDDGHENAHNSGLGLGVQVGGRYYFNDRLGLNLEFGGGFVSGGKIGISCKL"
     gene            9407..9637
                     /locus_tag="PLB85_00810"
     CDS             9407..9637
                     /locus_tag="PLB85_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU71895.1"
                     /translation="MITTERIETILDKIRPSIQADGGDVELVNIREDNVIEVRLKGAC
                     NGCPMATLTLKAGIERIIKEEIPEVAEVISVR"
BASE COUNT         2441 a         3068 c         2658 g         2041 t
ORIGIN      
        1 cggaacatcc caagtcccgc tacggcgacc tctacaagct tctgcaccag gcctattacg
       61 gtccaaccca catcgttccg gaccctgtgg ccatcgccac cgacatcagg gaggaaatgg
      121 ccgccatcca aacccaaaac tgcggcccca gccaggatat tggctgcgga aaaggcttcg
      181 tccggctcaa tcttgtcgcc ctcaccgtat ttcgtgatat ttccgggtca aaaacctcca
      241 acctgcttcg tgaccaacta ctccgcgatg ccatgcaaaa agtgaagccg gagaaggtga
      301 aactgcttac ccagagcatc ctggcgtcca ggctggaggg tgtttttacg cttcaggact
      361 ggcaaagcat ctggaaacaa gtccagccgt tggtttgtga gttcatcttc cccactttca
      421 ccgaacgggc cctgatcgaa caatgcctcg aaacggggct aatccctccg cattcagatg
      481 attaccgata tctctacaaa ccgcattacc gcgtcatcca ccacagccac ctggccaaat
      541 tcaaaaccca caaaggatag accatgaatc ccgtccgcgt ccgtttcgcg cccagcccca
      601 ccggattttt acacatcggt ggcctccgca ccgcccttta cgattacctt ttcgcacgcc
      661 acagcggcgg aaccttcatc ctccgcatcg aagacaccga ccgcaaccgc ctggtggaag
      721 gcgcggtgga aaacctcatc gcgtccctgg gccgcctggg catagagata gacgaaggtc
      781 ccggcatcgg cggggatttt ggcccctacg tgcagagcga acgcttggaa ctctatcaca
      841 gggaggcgca gaggctgctg gattccggcc acgcctaccg ctgtttctgc actccggaaa
      901 cccttgccca aatgcgcgaa gagcagcagc gcaaaggcga attcgtgaaa tacgaccgcc
      961 gctgcctgtc cctttccgaa gaggaggtga aggcccgcct ggccaataat gaaccccacg
     1021 tcgttcgcct gaaaatgcct gacaaccggg ttttcagctt caacgacatc atccgggggg
     1081 acgtggaaat ggatgccgcc cagtccgacg accaggttct ggtcaaatcc gacggcttcc
     1141 ccacctacca tctggcagcg gtggtggacg accattacat gcaaatttcc cacgtcatcc
     1201 gcggcgagga atggctctcc agcacgccca agcacatctg gctttacgaa tgcctgggct
     1261 ggaaagcccc gttctgggtg catctgcccc tcatcctcaa tcccgaccgc agcaagcttt
     1321 ccaagcgcat gaacgacgtt tcggtggaaa gctacctgca aaaaggctat ctgaaagagg
     1381 ccctgctcaa tttcgtggcc ctgctgggct ggcacggagc tgatgaccgg gaaacctaca
     1441 gcctgaaaga actttgcgag gaattcaccc tcgaacgggt gagcaaatcc ggggccgttt
     1501 tcgacctcac caaactggac tggatgaacg gctggtattt gcgcaacttg cctctggagg
     1561 aagtggtgga gagttccctg ccctggttcg aagaggaaaa gctgcccctt ccggacagcc
     1621 tcaccctttc cagaatcgtc gctgccgcgc gggaacgctg caccctgctg ccggaaatcg
     1681 cccaatacgc aaaaatgttc ctttgcccgg cagaactggg ccctgatgac ggggcctttc
     1741 tccaatctga agattcccgc agggtgctgc gctggttcca aagccagctt ccacctgatg
     1801 gcgaaattga cgccgccagc ctcgatgcca tcgtgaagag cggcatggcc gaacttggcc
     1861 tgaaaggcaa ggcttactac actccgctgc gcctggccct aatccaccaa cagcatggtc
     1921 ccgacctgcc caccaccttt gccatcctgg gtttagagga agtccacaga agactggcag
     1981 cggcgatacg ggattaaaac acgccattcc ttaatatcca tacacgcgga gccgggggaa
     2041 acaaacccgg ttggacgaaa cttctcccta ccccggccat tagccgtttc cggtccgctt
     2101 ccactacgcc tcccgcagaa ttcccgcatt tcaagcgggc attgtccggg aggcaaacat
     2161 gaggcggttt agatagggct aagggcgcga ccacggatgg caatatccaa agcgctttta
     2221 aataaaccgt ttcagccact ctgaggaata atatccacaa tcccgattca caaagccaaa
     2281 gattttactt gacttttcga ctgactggta ttactcggtc gaaacgttgg acagccaccg
     2341 ggtgaaagtc ttccggttcc gctccaacaa aggaaagaga ggtggatgat gaatccagtc
     2401 ccggataaaa aagaacgcat acttacagcg gcaacggagc ttttctcccg ctggggatac
     2461 gacaaaacgt ccctcgacga ggtggcggca agggcgcgaa tcgccaaggc caccatctat
     2521 tattatttcc ccagcaagga acagctcttc atagccgcct tcagcgccaa ggcggaggaa
     2581 cttttcgcca agctggacga ggagatagcc tccgcgagca gttttgagga caagctgtcc
     2641 tgctttctgc gcctgcccat gatttacgtt ttcaaacaca tgcccatcct tatcgaagcc
     2701 attcatcaaa tgcccacaga ttatctggga aacctggatg aataccgcaa caattaccgc
     2761 aaccaaatga acgccctgct ggccggcatc ctggataacg cccggagcgc cgggataatc
     2821 aatgaatggg tggattccca gcgtttcagc gagcttatca gcgactggtt catgctgggc
     2881 gacagctgga tcgacatcca ggaaaaagac aagatcatca ggcgcatcga acgcgaccac
     2941 gaactgatag tccagcttct gctgtacggc atcatcaagc cgtcggtgaa ggattcggcg
     3001 cgccgcaatt ccgacggagc caaaccatga aaagacatct gatcttcatg ctgcttctgg
     3061 cggctgtctg cctgaacggc atcaccctcg aggaaagcct tgagcttgcc agacaacgca
     3121 acagcacgct gctgatggca agggaggaaa tcaaccgggc ggagcaaagc aaccttgagc
     3181 tgaaaggcgg attcttcccc aaactcaccc tggccggagc ttacagcctt cagaggacgt
     3241 atttgccctc ctcggccatg tccgacccta tcgacctcac ccaactgctc aatccctaca
     3301 ccgccagcga caacgactac atgctggccg cttcggtttc cgggattgcc aattcacttt
     3361 tgcccagctc accgatggac gagggttcgc tcgcggcctc actgaagctg gagcaggtta
     3421 ttttcgccgg aggcaggctg cataacggtt acaaggccac ccagcgctac cgtgacatca
     3481 gccgcttgaa cctgaaggtg aaggaacagg acgtggccct gcaaaccatc caaatgttct
     3541 acggctacct gctcacgacc cgcctggtgc aggttcagga ggaagccctg gccactctgc
     3601 ggcgccacgt cgcgcaggtt gagcttttca accaggaagg cttcgtggcg gaattcgatg
     3661 tcctgcgggc cagactggaa gaggcaaagc tggagcccca gctaatgaag gccaaaaacg
     3721 accgagacct ggccctggcc gccttccgca aacaaatcgg cgacgagcag gaaaccaccg
     3781 tccccgagga tgaattcgcc ctgccggaac ggatcgacct gagtctggaa caagcccttg
     3841 cccaaggcct ggcgaaccgc ggagaactgg aaatggcggg attggccaca gagctgaaag
     3901 agttgatgtg gaaagctgag aaggggatct atatgcccac actcggcctg caagccagcg
     3961 cttccctctt caccgccgcc gacgaatttg ccatcgaagc cgatgatttc ggcaccaatt
     4021 acagcgtggg gatcggcatc tccatccccc ttttcgacgg catgtcaaac cgagccaagg
     4081 tcaaggccgc ccatcacgat tatctgcttt cccggataca gcaacgggat gccaccgccc
     4141 tgatccggct tgaaatcacc caaaactaca acaatctgcg ccacgccgag gaaaactacc
     4201 gcgtccagca ggaaaacatg cgcctggcag aacaggggct ggaactggcg cgggtccgct
     4261 atgaaaacca ggtggggatc caactggagg tctttgacgc ccagaccacc ctgtcagcca
     4321 tcaaactgca atattaccag gcgatctatg aagtgatatc tgccacccgg atgcttcaga
     4381 aatcactggg aaccactttg tgaacggaga caccatgaaa caaacaatta ttaccgtgat
     4441 ctgctgcctg gctttgctat tggcggcatg cggcggcaag gaccaagccg gcaagagcat
     4501 ggaacaactg cataaggaac agggaatccc cgtgcgcgtg aaaaccgtcg ccgcggaaaa
     4561 cttcagcaaa gacctcagct ataacgccac cctgggcggc agctccgaat cctacggcct
     4621 gtcgatgctg gcagaggttg tggcgggcgt gcatgccaaa gtgggcgaca gagtctcagc
     4681 cgggcaggtg attgtcactt tcccaaaaac aacacccagc gcccaattcg agcaggcctc
     4741 caccggctac aacgccgcca ggcaagccta tgaacgggtt aaaaacctcc aggcggcagg
     4801 cgctgtttcc cagcaggacc tcgacaacgc ggaaacccag ttcaaactcg cccaggccaa
     4861 tctggaagcc agccgccagc ttatcaccgt cacctcacct atcagcggag tgctgaccag
     4921 cctggctgtg aatcccggcg agatgagcca tccgggccag atactctttg ccgtttccgg
     4981 aaccggcggc tacaaagcca agttcaccgt tcccgacaaa gtggcgcgca gcatcaaaat
     5041 cggaacttct gccaccgcca ccatccggga tatggaactg acgggacggg taagcaaaat
     5101 ctccctcgcc ctggacccct acacccaggc ggtgcctgtg gaagtgagtt taccagcggc
     5161 ggggcagcgc atcagctacg gcgccactgc cgaaatcagg ctgcacaccc agtcgcaggc
     5221 tgacgccatc gttgtgaacc gcgaacacat cgtcaccgaa aatggacaaa aatacgtgtg
     5281 ggtggatgaa aagggcaaag cggccaaacg ccccatcgaa accggaatgg acaaccagct
     5341 ccagttcgaa gtggtatcgg gcctccagcc cggtgaccag cttatcacag agggaatcaa
     5401 cctgctttcg gaaaacgcgc gcatccgcgt ggtgaaatag aggaggcggc atgattcttt
     5461 ccgacctctc agtaaaacgc cccgtgctga tcagcatggt cgtgctggtc ttcgtggttt
     5521 tcggtgtcct ggcctggttc gcgctgccgc tgaacctgat gccggaggtc aatctgccct
     5581 tcatcgtcat ccaaacggtc tatcccggcg cggggccttc cacagtggaa acccaggtca
     5641 ccaaaaagat agaggacgcc ctcgccaccg tcagcctggt cgattacacc cagtcctatt
     5701 cgcttgataa cgccagcatc atcatggtgg cattcaaaat gaagaaaaat gtggatatcg
     5761 ccaaccagga agtcaaagac aagatagatg ccatccagcg cgacctcccc gacgccatcg
     5821 aaaagcctat cgtgatgaag ctgaacatga acgccgtgcc cttcatggac atcatattga
     5881 gcggaaacat ggacggccgg gagctctacg aactggcaga caccaagctg aaagaccgct
     5941 tcggccagat tgcaggagtt ggtgaagtga gcattaccgg aggcgccaaa cgccagatag
     6001 aggtgaccct caaggaccgg attgtccatg aaaacaggat atcgatgccc cagcttctgc
     6061 aactccttgc cgcgcagaac attgacatgc cggcgggaaa cttcaaacgc ggttcgcagg
     6121 aatactcagt gcggctgaaa ggccaataca ccagcctgga taaaatcgcc gacgccgaca
     6181 tacccacggc ctacggcatg aaaaaactgc gggacctggc cgaaatcaca gacggcgccg
     6241 aggaagtgcg cagccgggcc atctaccaca acgtggaagc caaatccatc catgacaaca
     6301 tagtccgcat ctcgcttacc aacgcccccg acggcaacgt ggtgaacatt gccaaagagg
     6361 tgaggcagat gatggatgaa atcgaggctg acctgcccgc gggtgcttcc ctggacatcg
     6421 ttcgcgacga ttcagatttc atacgcggaa cggtggacga cactttctcg aacatcctgc
     6481 tgggaatcct gttcacagcg ctggtgctgc tgattttcct ccacgactgg cgttccaccc
     6541 tcatcgtggg cctgtccatg cccatttcca tcatcgccac cttcatgttt atgcagattg
     6601 ccggctacag cttcaacctg ctcaccctca tcggcatttc ctgctcggtt ggggtcctgg
     6661 tttcaaactc agtggtggtt atcgaaaaca tcttccgcta caaggaactt ggcaaaaacc
     6721 gccgcgatgc cgccatgcag ggcacttcgg agatagccct ggccgtgatg gcaagcaccc
     6781 tcacaaacat cgtggtgttc attccgatag cctcaatgac cacccttgtg gggcagtttt
     6841 tccgtgaatt cgccctcacg gtcaccttcg ccaccatttt ttccctcgtc acgtctttca
     6901 cggtcacgcc catgcttgct tcccgcatcc ttaaagaagg cagcacgcaa aaccgctttg
     6961 gcatcaaatt cgacaagctg ttcgatagat tctccgcctg gtacaaacgc tttctcggac
     7021 gcgtgctaag ctcgaaaaaa cgcagccggg gaatcattat catcaccgtg gcggttctgg
     7081 tggtcagctt tgccctgatt ccggtggtgg gtctggaatt catgccggaa atggaccagg
     7141 gggcaatgtc cctgacggtg gagcttcccc agggctccaa cctggaccag accgcctcga
     7201 ccctgcaaac catccacgag cgcctgggca aacaccccga aatcagccac gtgatcacta
     7261 acctcggttc cgctggctac gtggacaccg gcaccaacct ggcttccagc agcatcaaac
     7321 tggtggagcc aaaccgccgc aagctgaatt caaaacaact ggcggaccgc gtaaccaggg
     7381 aactggcgga catccccaac gcccggatca aggtgagcgc cgtcagctcc ggattcggcg
     7441 aggacaccgg cttggaattc tatctccagg gacaggacaa cgcccgcctg gaacagctca
     7501 aaaccactat caccaaccgc atcaaggacg cgcccggcct catcaacctg gacaccagca
     7561 cccgcggcgg aaggtccgaa ctcaccatca ccccgaaacg ggcggagatg gcggcggtgg
     7621 gagccaccgt ttacgacctc gccctggccc tgcgtgccgc tgtcgaaggc atggtctcca
     7681 cccagtatca cgaaggcgac aaccagtatg acatcaagct ttcgctggag gacgccgctg
     7741 tggattcccc ggataaaatc ggcaacctca gcgttgtggt gaactaccag ccctacctgc
     7801 tttcccagtt ggcggatgta tccttcgggg aggggaccaa ccgcatcacc caccgcgacc
     7861 gcagcaaggc ggtggtcttc accgccgggg tcgccgaggg agccaccatg ggcgacgtga
     7921 tttccgctgt caaggagcgt ttggaagatg tcgaactgcc ccgcggctac aagatagttt
     7981 ggggcggaag cgcggaaatg ctcggcgaaa tggtgaaaga catgctcaaa accctcatcc
     8041 tggcggtatt gctgacatac atgctgctgg cagccatgct cgaaagcctc gtccagccgc
     8101 tcttcatcct gggaacggtg cccctagccg tgatcggcgt catcttctcc ctgctgattt
     8161 cgggacagac cttcaacctg gtctcattga tgagcatcat catgctggtg ggaatcgtgg
     8221 tgaacaacgc cattttgctg ctcgattacg cgaacctgcg ccgcaaacag ggaatctctg
     8281 cttacgaagc cctgatggaa gccggggaga caaaactcaa gcctatcatc atgagcaccc
     8341 tggccatcgt tatcggaatg ctgcccatgg ctctgggagt cggtcccgcc ggcaaggaac
     8401 tccgaatgcc gatgggaatc gtgatgatgg gcggcttgct cgtttccgcc ttcctcaccc
     8461 tggtcatcat cccggcgttt tattacctga caacaaagca ggccaaatcc gactgatata
     8521 aagcctccgg gctttgctga agggccttgc cggttttccg tcctgcgcgg attttccggc
     8581 aaggctcttt tttaccgact gaacctttgg ctggctccat cccgcctttg ccaaacattc
     8641 cggggctgga tgcccttgtg aagtgggctt atcgaagccg ttgccttcgt ttgccaagct
     8701 ggcttgggtt cggccgggca aagatttgtt tgcccccgcg atggaacggc ttaatctatt
     8761 catattggta agacaaaaga atcggttccc tgccgcaacg gacagggagc aataaacgta
     8821 aaaaggataa gaaatgaaaa aggcactgct tttactcgtt cttctgattg gcgcggcctc
     8881 cgtgttcgcg ctgcccctaa acgttggcca gtcgcagttt aacatcggaa ctggcttttc
     8941 cacttggggc atgcccatct atatgggtgt cgattatggc gtcaccccgg atatcaccct
     9001 gggcggcgag gctaccctgc gcttccacaa tcacggctcc tgggtgggca tctatggcaa
     9061 cggcaactac cactttgtca atctgctcaa catgccctcc aacacggact tctatgccgg
     9121 cctctgcctc ggcttcgatg tctggttgcc cgacgatggc catgaaaatg cacacaactc
     9181 agggctcggc ctgggagtgc aggttggcgg acgctattac ttcaatgatc gcctgggctt
     9241 gaacctcgaa tttggcggcg gattcgtctc cggcggcaag atcggcattt cctgcaaact
     9301 ctgacccctg cctcacagag cgggataata tcggcgcggg agatttgtct tccgcgcttt
     9361 tcctgttatc atatcggaag caattaattc ttaataagga accaaaatga tcacaacgga
     9421 aagaatcgaa accatattgg acaaaatccg cccctccatc caggccgacg gaggcgacgt
     9481 tgaactggtg aatatccgcg aggacaacgt tatcgaggtt cgcctcaaag gcgcctgcaa
     9541 cggctgtccg atggccaccc taaccctcaa ggccggcatc gaacgcatca tcaaggaaga
     9601 aatccccgaa gtggcggaag tcatctcggt gagatagctc taaaacccca ccctcaaacg
     9661 gttataccgg aacccaggcc aagcttgggc tccggttttt tgttcaagtt aatttaggct
     9721 cactttcaaa ccgggtgggt aatccggtta atcaagcccg tagggcgaca taacgttagc
     9781 gagggtgtgt agtgagcctt atttttccgc acgagtgaaa atgcctcccc ctatgaacgt
     9841 gcgtatgaga aaaatgggtt ttccggattt attttgcgtc tccccgaaaa agagcctcat
     9901 acagattaca cggattgcgc ggatgaccgg tagcggccct gaaaggaatt ggcggacggc
     9961 ttgaaacccc gtccgctttt tctttcactg tttcagatat atacagacct tgagctatga
    10021 tatcacaact gcgatttcag aaaaaaagct tgacagcata tagtcaattg aaaattatgt
    10081 atctgctgcc catttgccaa tggctaactg ggttaagatg tcgttacggc ggcatctttt
    10141 tgtattctat gtatcgatca tcgtagtatt ttctgttctt tggaagtaca taaaaagcag
    10201 taaccaaa
//
LOCUS       DAOOWR010000013        10100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6662_length_10100_cov_4.016625, whole genome shotgun sequence.
ACCESSION   DAOOWR010000013 DAOOWR010000000
VERSION     DAOOWR010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6662_length_10100_cov_4.016625"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLB85_00815"
     CDS             <1..852
                     /locus_tag="PLB85_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71896.1"
                     /translation="DGNVKVNNDSLSLAADSLTYYRIPDELNAGGRVLIEQKTKGKVT
                     RWFRSDKAIYSKFHNTVTVWSNVSSFDQEENATLTCGYAFWDRKNRYAYMIEEPRLDV
                     AGKDTLHVQADKFEYFEEERKLMATFNVLARSQDYEASSDFLVYLLKEEKAMFLGEPQ
                     FRSDSAIASAKEFYLFFKDRELQRAELVDSCRLDFAEEEDGPKKNWVKANYVRVDFED
                     KKIRRFEAEKTVSYRFDQEQTEERDFFVNTADGEFLEAKFNEESELEFMNMRQKIKGV
                     YKFHNES"
     gene            885..1634
                     /gene="lptB"
                     /locus_tag="PLB85_00820"
     CDS             885..1634
                     /gene="lptB"
                     /locus_tag="PLB85_00820"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOU71897.1"
                     /translation="MEAHKIQAQNLVKIYGRRKVVDGLSMEMSQGEVVGILGPNGAGK
                     TTTFYMILGLAKPNRGKVLLNGRDITHQPMYRRARLGLGYLAQAPSIFAKLSVRDNIM
                     AILQTLKISRQERKERLESALEELNLTSLANQKAYTLSGGERRKLEITRSLVTKPTFI
                     FMDEPFAGVDPIAVADIQDIINKLREKNIGVMLTDHNVIETLKIVNRAYIIFEGKIIV
                     SGSSLELINDEKAKKLYLGERFLSNPFEPQS"
     gene            1631..3082
                     /gene="rpoN"
                     /locus_tag="PLB85_00825"
     CDS             1631..3082
                     /gene="rpoN"
                     /locus_tag="PLB85_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU71898.1"
                     /translation="MSNLSQNISLKQRQELALKPKMLQSLKMLSLPILELEDYIKQEL
                     EQNPLLELREEKDDEEQDNLETASKESKESLDDSQNSDELGQTLNEARELTEILDQWN
                     DYHLGYEGWQGGTEADQGESLIRYEENAREKFLQQLYPLSLLENEVEFASELIDSCNS
                     YGFLPPNFDLGKVARAFGICPKRAEQVHQIILQLSPKGITARGISECLLAQLTDEQRD
                     NRILVGLCSDQFENLIHRRYQKIASHFGVGEDYILTMKEIVAKLDPKPGLRILTPNAA
                     YVYPDITIKRIDGKFEVIINDHVTPNIIISPRYRQMINRGYFDRDTLKFVRDRINSAK
                     FLIKSIYMRTRTLQRVAQSIIKFQPDFFQLGNGLIHPLTYAVIARDLSVSESTISRVV
                     KHKFAETPSGIYALKDFFSSTAGIDNNFEGISRQHVKTQISHMIDNENKKHPLSDQDI
                     VELLRAEGLNISRRIVAKYRSELGILNSRLRRQ"
     gene            3094..4494
                     /gene="lpdA"
                     /locus_tag="PLB85_00830"
     CDS             3094..4494
                     /gene="lpdA"
                     /locus_tag="PLB85_00830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU71899.1"
                     /translation="MPEYKVLIIGGGPGGYETAIRLNQYGIDCAVLEMERLGGVCLNW
                     GCIPTKALVKSSELFTELKNSIDYGLPPMEPSLDYGKVFARKNVVVEQLVGGVEHLFR
                     KRAIPVLRGKAVSVSVLDNSYEVLTEAGTSIRAEYLVLATGSVPKELSGLKIDEVNVL
                     SSTGILALDTLPKSLAIVGGGVIGCEFASIMNSFGVETTIIEFLPRIMATEDEEMVKR
                     LTLALKKAGIKIITGVGVESISQTGEGNELTLSDGAKLTFDKVLLSVGRVPRCGLDWL
                     NGAPDMEKGAVVIDSLMRSSLPGVYAIGDLTAKLPLAHTASKQGMIVAAHIRALSEGL
                     EYTDPELNYVNIPRCTFTHPELASVGYTEAEAKEKFGGIKVGKFPYSANGKALAMSGT
                     YGLVKTIAREDDDTLVGMHILGLNAAELIAQGALLLSLGAKADVAEKVTFAHPTLSET
                     IKESLEDLHNLAINKV"
     gene            4510..5046
                     /gene="raiA"
                     /locus_tag="PLB85_00835"
     CDS             4510..5046
                     /gene="raiA"
                     /locus_tag="PLB85_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU71900.1"
                     /translation="MQITITARRFELTKAIRDYVESSCLKINRYFDNIINVHVTLSLE
                     NSRNICEISLQASKFGLQSQAEEMDMYLSIDNALDKMEAQIKKLKDRVTDHQKRALKD
                     QFSEFSRETDFLVNPGNRDRKTVKTKRAVAEMLTVEEAMAKIETQKDEFLIFKNVETD
                     RLNVLVKRDEQNYKLFEP"
     gene            5110..6087
                     /gene="hprK"
                     /locus_tag="PLB85_00840"
     CDS             5110..6087
                     /gene="hprK"
                     /locus_tag="PLB85_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOU71901.1"
                     /translation="MKSITIRDFFDSKKKDLALSLITEPQTLNKNLSSPHINRPGLAL
                     AGYLDVFSTECIQIFGEVEVRYLQSLNEDMMLERARSIFKLDIPCIIITKGLTFPPAL
                     EYLANDLQIPILSSRLSTNQLIQQLTRYLQYTFAMEKTVHATLIEVFGLGILLSGKSG
                     IGKSECALDLIHRGHSLVGDDVITIRYLDEQLVGKSARDFGHFMEIRGVGFINVERMF
                     GIERVRKQKNIDFQIELMPWAENMDYERVGLTTNYADHLGVKIPIIYLPVSPGKNVSV
                     IVEVAAMNMILKGVGYDAAEDFNRQVHDEIRKQSFVKPSSLTLNASDKD"
     gene            6068..6334
                     /locus_tag="PLB85_00845"
     CDS             6068..6334
                     /locus_tag="PLB85_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOU71902.1"
                     /translation="MRQIRIKVTNPLGLHARPSTLIVRAATKYISDFYIEKDNMRVNG
                     KSIMGVMMLAAECGSVLNLIADGVDEEYLLEEIETLINSGFGED"
     gene            6341..8062
                     /gene="ptsP"
                     /locus_tag="PLB85_00850"
     CDS             6341..8062
                     /gene="ptsP"
                     /locus_tag="PLB85_00850"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOU71903.1"
                     /translation="MRAISGVVVQPGLAIGRACRIAPQKWVISQLKIKRGEVASELDA
                     LQAALTTVEAELEQQLNEFTGADEDREILSSHLLILRDPELLPAIREQVSTHLSSAAA
                     SVQREFEKVLDHFSVLNDPYFAQRAADFRDVRDRLLGELTGVRTTSLEDWESDQIAVL
                     EEVSPSLVSSFGRHEVQAYCSEHGSYTSHASILTRSTKITAVTGLAGLCDSVKDGETI
                     ILDGLEGKVILAPDPATLHFYEQMMERQRLQNARSQSEAGLPTTTLDGRWIKLRLNLD
                     LLPELGAKLELPADGIGLYRTEFLYLGKDELPSEELQFETYRRVAEQIVPHSVIIRTF
                     DLGGDKLSHLIPSSHEENPYLGIRGIRFSLAHEDVFRVQLRAVLRASAFGRIKLMFPM
                     VSDLRDFLRASQIVQECKEELTQEGLDYDAKIQLGVMIEIPSAALCADELAQNCDFLS
                     IGTNDLVQYTLAADRNNDALTPWYQTHHPAVLKLLRLTLAAGQKHRKPVSICGEMASH
                     TQYLPLLIGLGFTELSVGALAWLNCKSIIRRCDARLTELVQSADLDSLGEIENLIYDL
                     LKPYSTL"
     gene            8073..9428
                     /locus_tag="PLB85_00855"
     CDS             8073..9428
                     /locus_tag="PLB85_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU71904.1"
                     /translation="MLRFEHFNLLHSEALPTAIPAAKVKDYYYACMQAHEEIQADRHS
                     NILGFYDLPEYGVRPIEDFVASLDPRFETLVVLGIGGSALGNRALYSALRTERELPRR
                     VLVYDNVDPVFLHEILAQIDLDTTLFNVITKSGTTAETMAGYMILADLIRKRYPADYT
                     KRLIITTDREKGFLRQVIKDEGYPSFVVPDNVGGRFSVLTDVGLLSSAFAGIRIRELL
                     SGAASMRSRCENLDAFGNPAYLNGLLHYLYMREGKNISVMMPYSNSLYDLADWYRQLW
                     AESLGKRHDVKGREIMVGQTPVKALGTTDQHSQVQLYTEGPNDKVFTFLTVENFAHDF
                     SIPNLHPDRAEVSYLGGRKLSELLNAERLATEIALTKANRPNCNIIFPALDEFHLGEF
                     IMMFEIQTVFTGKLLHINPLDQPGVEAGKIATYALMGKAGFDKEREEIKQYIEDRKQQ
                     Y"
     gene            9662..>10100
                     /locus_tag="PLB85_00860"
     CDS             9662..>10100
                     /locus_tag="PLB85_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71905.1"
                     /translation="MKKIVLLFVVLSILFLTSCSIIGINSVTSKTKDIYGSVIQLPVV
                     VDLIVQNTKITKEFTTECPQNPTDDVIEDVKSIAIAAALKEHSADVLIEPNYDVQITS
                     KSASNSEIKVVVTGYPATYKNFRNATKGDVELIEGTTNIRMLPT"
BASE COUNT         2597 a         2817 c         2523 g         2163 t
ORIGIN      
        1 gacggcaacg tgaaggttaa caatgattcc ctcagcctgg cagctgacag cctcacctat
       61 taccgtatcc ccgatgaact gaacgcgggg ggcagggtgc tgatcgaaca aaagaccaag
      121 ggcaaggtta cccgctggtt ccgttctgac aaggccatct acagcaagtt tcacaacacc
      181 gtgactgtct ggagcaatgt gagttccttc gaccaggagg aaaacgccac actcacttgc
      241 ggctatgctt tttgggaccg caagaaccgc tacgcataca tgattgagga acccaggctg
      301 gatgtagccg gaaaagacac tctgcacgtg caagcggata aatttgaata ctttgaggag
      361 gagcgcaagc tgatggccac attcaacgtt ctggcacgca gccaggatta cgaggccagc
      421 agcgatttcc tggtctatct gctcaaggag gaaaaggcga tgtttctggg cgagccgcag
      481 ttccgctctg acagcgccat cgccagcgcg aaggagtttt atctgttctt caaggaccgc
      541 gagttgcagc gggctgaact ggtggactcc tgccgccttg atttcgcgga agaggaggat
      601 ggcccgaaaa agaattgggt gaaggcaaac tatgtccgcg tcgattttga ggacaagaaa
      661 atccgccgct ttgaagccga aaagacggtt agctaccgct ttgaccagga acagacagag
      721 gaacgcgact tttttgtgaa tactgccgac ggtgagtttc tggaagctaa gttcaacgag
      781 gaaagcgagc tggaattcat gaacatgcgc caaaagatca aaggggttta caaattccac
      841 aacgagtctt gacaaaaccc gctttcgggg tattataaga gtatatggaa gctcacaaaa
      901 ttcaggccca aaacctggta aagatatacg gcagacgcaa ggtggtcgat ggtctcagca
      961 tggaaatgag ccagggtgag gttgtgggaa tccttggccc caacggcgcc ggcaaaacca
     1021 ccacctttta catgatcctc ggcctagcca agccgaaccg gggcaaagtg ctgctgaacg
     1081 ggcgggatat cacccaccag ccaatgtacc gccgggccag gctgggtctg ggttatctgg
     1141 cccaggcgcc ttccatattc gccaaactct ccgtgcgcga taacatcatg gccatcctcc
     1201 agaccctaaa aatcagccgc caggagcgca aagaacgcct ggaaagtgcg ctggaggaac
     1261 ttaacctcac ctccttggcc aatcagaagg cttacacgct ctccggaggc gagcgccgaa
     1321 aactggaaat aacccgctcc ctggtgacca aacccacctt catcttcatg gacgagcctt
     1381 tcgccggggt cgatcccatc gccgtggcgg atatccagga catcatcaac aaactgcggg
     1441 aaaagaatat cggtgtgatg ctcaccgacc acaacgtaat tgagactttg aaaatagtca
     1501 accgggctta tattatcttc gaaggaaaga tcatagtatc aggttcttca ctggagctga
     1561 taaatgatga aaaagccaag aaactatatc tgggagaacg cttcctgtcc aatccgtttg
     1621 agccgcaatc atgagcaacc ttagccagaa catatccctc aagcaaaggc aagaactggc
     1681 gctcaagccc aagatgctgc aatccctgaa aatgttgtct ctccccattc tggaactgga
     1741 agactacatc aagcaggaac tggagcaaaa ccccctgctt gaactcaggg aggaaaagga
     1801 tgatgaggag caggacaatc tggaaacggc gtccaaggaa agcaaggaat ccctggatga
     1861 ttcgcaaaac agtgatgagc ttggccaaac cttgaatgaa gcccgcgaac tgacggaaat
     1921 ccttgaccag tggaacgatt accaccttgg ctatgaaggc tggcagggcg ggaccgaggc
     1981 cgaccagggc gaatccctca tccgctatga ggagaacgcc cgggagaaat ttctccaaca
     2041 actttatcca ctctccctgc tggaaaacga ggtcgaattc gcttctgaac tgatcgacag
     2101 ctgcaacagc tatggctttt taccgcccaa tttcgatttg gggaaagttg ccagggcttt
     2161 tgggatttgt cctaaacgcg cggaacaggt acaccagatc atcctccagc taagccccaa
     2221 aggcatcacc gcgcgcggca taagtgaatg cctgctagcc caactcacgg atgagcagcg
     2281 cgacaaccgt atcctggtgg gcctttgctc cgaccagttc gaaaacctta tccaccgccg
     2341 ctatcagaag atcgcttcgc atttcggggt gggggaggac tatatactga ccatgaaaga
     2401 gatcgtggcc aagctggatc ccaaacccgg cctgcgcatc ctaactccca acgctgcata
     2461 cgtatatccg gacatcacca tcaagcggat cgacgggaaa ttcgaagtga tcatcaacga
     2521 tcacgtaacc cccaatatca tcatcagtcc ccgctaccgc cagatgatca accgcggcta
     2581 ctttgaccgc gacaccctta aatttgttcg tgaccgcatc aacagtgcca aattcctcat
     2641 caaaagcatc tatatgcgca cccgcaccct tcaacgcgtg gcccagtcca taatcaaatt
     2701 ccaacccgat tttttccagc ttggaaacgg cctcattcat ccgctcacct atgccgtgat
     2761 cgcccgcgac ctatctgtaa gcgaatcaac catttccaga gttgttaagc ataagtttgc
     2821 cgaaaccccc tccggcatct acgccctgaa agatttcttc agcagcaccg ctggcattga
     2881 caacaacttt gaaggcatct cgcgccagca cgtgaaaaca cagatcagcc acatgataga
     2941 taacgagaac aagaagcatc ccctttccga ccaagacatt gtggaattac tgcgcgcgga
     3001 ggggctgaac atctcacgcc ggatagtggc caaataccgt tcggaactgg gtatcctgaa
     3061 cagtcgcctg cgccggcaat aaaggaggat tttttgcccg aatacaaagt tttgatcatt
     3121 ggaggcgggc cgggaggcta tgaaaccgca atccgcctca accaatacgg catcgactgt
     3181 gccgtgcttg aaatggaacg cctcggcggc gtttgcctga actggggctg cattcccacc
     3241 aaagctttgg tgaaaagctc cgagctcttc acagagctga agaactcgat tgattatggt
     3301 ctgccgccaa tggaacccag cctggattac gggaaagtct ttgcccgcaa gaatgtcgtg
     3361 gtggaacaat tggtgggagg agtggagcac ctttttcgta aacgcgccat cccagtgctg
     3421 aggggaaaag cggtcagcgt ttccgtcctt gacaatagtt acgaagtgct tacagaggct
     3481 ggaacatcca tccgggctga atatctcgtg cttgccacgg gttctgtgcc caaggaactt
     3541 tccggcctga aaatcgacga agtgaatgtg ctttcttcca ccggcatcct ggctttggac
     3601 accctgccca aaagcctggc catcgttggc ggcggagtca tcggctgtga attcgcctct
     3661 attatgaata gctttggggt cgaaaccacc ataatagaat ttttgccccg catcatggca
     3721 acggaagatg aggaaatggt caagcgcctc accctcgccc tgaaaaaagc cgggatcaag
     3781 atcattaccg gagtgggcgt ggaaagcatc tcgcaaaccg gcgagggcaa cgaactcacc
     3841 ctgtctgatg gcgccaaact gacattcgac aaagtattgc tcagcgtggg ccgtgtgccg
     3901 cgctgcggtt tggattggct gaatggcgca ccggatatgg aaaaaggggc tgtggtcatc
     3961 gattcgctta tgcggagcag cctgccagga gtttacgcca tcggtgacct cactgccaaa
     4021 ctgcctttgg cccacacagc cagcaaacag gggatgatcg tagccgcaca catccgcgcc
     4081 ctcagtgaag gcctggaata tacagacccc gaattgaatt acgtgaacat cccacgttgt
     4141 actttcaccc atccggagct cgcctccgtg ggctacacag aggctgaggc caaggaaaag
     4201 ttcggaggga tcaaggttgg gaaatttcct tacagcgcga acggcaaagc cctggccatg
     4261 agcggaacct acggcctggt caaaacaatc gccagggagg atgatgacac gcttgtgggc
     4321 atgcatatcc tgggcttgaa cgccgctgaa ctcatcgccc agggagccct gttgctgtcg
     4381 cttggagcga aggccgacgt tgcggaaaag gtcacttttg cccatcctac cctatctgaa
     4441 accatcaaag aatccctcga ggatttacat aatctggcca ttaacaaagt atagaaggag
     4501 gaatcacaca tgcagatcac gatcacagcc cgtcgattcg aactcacgaa agcgatcagg
     4561 gactacgtgg aatcttcatg cctgaagatc aacaggtatt ttgacaacat catcaatgtc
     4621 cacgttaccc tgtctctgga gaacagccgc aacatctgcg agatctctct ccaggcctca
     4681 aaattcggcc tgcagagcca ggcggaggaa atggacatgt acctttccat cgacaacgcc
     4741 ctggacaaga tggaagctca gatcaagaag cttaaagacc gtgtgaccga ccaccagaaa
     4801 agagctctca aagaccagtt ttcggaattt tcccgcgaaa ctgatttcct ggtcaatcct
     4861 ggaaaccgag accgcaaaac agtaaaaacc aaacgtgctg tggccgagat gcttaccgtc
     4921 gaggaagcca tggcaaagat cgaaacccag aaagatgagt ttctcatctt caaaaatgtg
     4981 gaaaccgacc ggctgaacgt cctggtgaaa agggacgagc agaattataa actctttgaa
     5041 ccgtaagcag ttgcccgcgg ccgccggcag cactgccttc cggcggcctc atcgggctgg
     5101 ggaccgacta tgaaatccat cactatccgc gatttttttg acagcaaaaa gaaagacctt
     5161 gctctttccc tgatcaccga gccccaaacc ctgaacaaga atctcagctc gccccatatc
     5221 aaccgtccgg gtctggccct ggctggttat ctggatgttt tttccaccga atgcatccag
     5281 atattcggtg aggttgaggt gcgctatctg caaagcctga atgaagatat gatgctggaa
     5341 agagcccgct ccatcttcaa gctggatatc ccctgcatca tcatcaccaa aggactcact
     5401 ttcccgccgg cgctggaata ccttgccaac gacctgcaga ttcccatcct ttccagccgg
     5461 ctttccacca accagctaat tcagcaactt acccgctatc tccaatacac ctttgccatg
     5521 gaaaagacag tccacgccac gctcatagaa gtttttggcc ttggaattct gctttccggc
     5581 aaaagcggga tcggcaaaag cgaatgcgcc cttgatctca tccaccgcgg gcacagcctc
     5641 gttggtgacg acgtgatcac catccgctat ctggatgaac agttggttgg caaatccgcc
     5701 cgcgatttcg gccatttcat ggagattcgc ggcgttggct ttatcaatgt ggaacgcatg
     5761 ttcggcatcg aaagggtgcg caagcaaaag aacatcgact tccagatcga gctgatgccc
     5821 tgggcggaaa acatggatta cgaacgggtt ggcctcacca caaactacgc cgaccacctc
     5881 ggggtgaaga ttcccatcat ctatctgccc gtgtcgccag gcaaaaacgt ctcggtgatt
     5941 gtggaagtgg cggccatgaa catgatccta aaaggtgtgg gttatgatgc cgctgaggat
     6001 ttcaaccgcc aggtgcacga cgagattcgc aagcaaagct ttgtgaaacc aagcagttta
     6061 acccttaatg cgtcagataa ggattaaagt aacaaaccct ctcggcctcc atgcccggcc
     6121 ctcaaccctg attgtgcgcg ccgccaccaa atacatcagc gatttttaca ttgagaaaga
     6181 caacatgcgg gtgaacggga aaagcatcat gggtgtgatg atgctggccg cggaatgtgg
     6241 ctccgtgctg aacctgatcg ccgacggagt ggacgaggaa taccttttgg aagagatcga
     6301 aaccctcatc aacagcggct ttggagagga ctgaaaagat atgcgcgcca tcagcggagt
     6361 tgttgtccag cccgggcttg ccatcggaag ggcgtgccga attgcaccgc agaaatgggt
     6421 gatttcccag ctgaagatca agcgcgggga agtcgcctcc gaactggatg ccctgcaagc
     6481 cgcgctaacc acagtggaag cagaattgga gcaacaactt aacgaattca ccggagcgga
     6541 cgaagaccgc gagatactta gttcccacct gctcattctt cgtgatccag aactgcttcc
     6601 cgctatccgg gaacaggttt ccacgcatct gtcctctgct gcggcttcgg tgcagcgcga
     6661 gtttgagaaa gtgctggacc atttttccgt actcaatgac ccctatttcg cccagcgcgc
     6721 cgccgatttc cgcgacgtgc gtgaccgtct gctgggcgaa ctcactggcg ttagaactac
     6781 cagtctggaa gactgggagt ctgaccagat cgcggttttg gaagaagtca gcccttcgct
     6841 ggtcagttcc ttcggacggc acgaagtgca ggcttactgc tctgaacatg gcagttacac
     6901 ctcccacgcc tccatcctta cccgctccac gaaaatcacc gccgtcacag gcctggctgg
     6961 gctctgcgat tctgtaaaag acggcgagac catcattctg gacggcttgg aagggaaagt
     7021 gatcctcgcg ccggaccctg caaccctcca tttttatgaa cagatgatgg aaagacagcg
     7081 cctgcaaaac gcgcgttccc aaagcgaggc agggttgccc accacaaccc tcgacggccg
     7141 ctggatcaag ctacgcctca atctggacct gctccccgag ctgggcgcga agctggaact
     7201 gcccgccgac ggcataggcc tctatcgcac ggagtttctt tatctgggca aggatgaact
     7261 gccttccgag gaattgcagt ttgaaactta ccgtcgggtg gcagaacaga ttgtgcccca
     7321 tagcgttatc atcagaacct tcgacctcgg cggcgacaag ctttcccatc tcataccttc
     7381 ttctcatgag gaaaacccct atctgggcat ccgcggcatc cgcttctctt tggcccatga
     7441 ggatgttttc cgcgtccagc ttcgcgcagt tctccgggcc agtgcttttg gccggataaa
     7501 gctgatgttt cccatggtga gcgatctccg cgatttcctc cgcgccagcc agatagtgca
     7561 agagtgcaag gaagaactga cccaggaagg cttggattat gacgcaaaaa tccagcttgg
     7621 agtgatgata gaaatccctt cggcggccct ctgcgcggat gaacttgccc aaaactgcga
     7681 tttcctcagc atcggcacca acgacctggt gcaatacacc ctggcggcag accgcaacaa
     7741 cgacgctctc acaccctggt atcaaaccca tcatccagcc gttctgaaat tgctgcgcct
     7801 caccctggcc gccggccaaa agcaccggaa gccagtctcc atctgcggag agatggcttc
     7861 ccatacccag tatttgccgc tgttgatcgg actgggcttc acagagctga gcgtgggtgc
     7921 cttggcctgg cttaactgca aaagcatcat ccggcgttgc gacgcccgtc tgaccgaact
     7981 tgtccaaagc gctgacttgg acagcctcgg cgaaatcgaa aacctcattt acgacctttt
     8041 gaaaccctat tccaccctat aaggagagcg ccatgctgcg tttcgagcat ttcaacctct
     8101 tgcattctga agctttgccc acagccatcc ccgctgccaa ggtgaaggat tattattacg
     8161 cctgcatgca ggcccacgaa gagatccagg ccgaccgcca ctccaatatc ctgggctttt
     8221 acgacctgcc ggaatatggc gttcgcccca tagaagattt cgtggccagc ctcgatcccc
     8281 gtttcgaaac cctagtggtg ctgggcatcg gcggttccgc cctcggcaac cgagctctat
     8341 attccgcgct gcgaacggaa cgcgagctgc cccgccgggt tttggtttac gataacgtcg
     8401 atcccgtttt cctgcacgaa atcctggccc agatcgacct cgacaccacc ctcttcaacg
     8461 tgatcaccaa aagcggcacc accgccgaaa ccatggctgg atacatgatt ctggcagatc
     8521 tcatccgcaa gcgttatcca gcggattaca ccaaacggct gatcatcacc accgaccgcg
     8581 agaaaggctt tctgcgccag gtgatcaaag acgaaggcta tccctccttc gtggtgccgg
     8641 acaacgtggg tgggcgcttt tccgtgctta ccgatgtcgg cttgctctca tcagctttcg
     8701 cgggcatcag gattcgcgaa ctcctcagcg gtgccgcctc catgcggtcg cgctgtgaaa
     8761 accttgatgc attcggcaat ccggcctatc tgaacggtct gctccactat ctttacatgc
     8821 gagagggcaa aaacatctct gtgatgatgc cctattccaa ttcgctttac gacctcgcgg
     8881 actggtatcg ccaactctgg gccgaatctc tgggcaaacg ccacgacgtc aagggccgcg
     8941 aaatcatggt tggccagacc ccggtgaaag cgctgggcac cacggaccag cattcccaag
     9001 tgcagcttta caccgaaggc ccgaacgaca aagtctttac tttcctcacg gtcgaaaact
     9061 tcgcccacga tttcagcatt cccaacctcc atcccgaccg cgcggaggtc agctacctcg
     9121 gcggcaggaa actttccgaa ctacttaacg ccgaacgcct tgccaccgaa atcgccctca
     9181 ccaaagccaa ccgccccaac tgcaatatca ttttccccgc cctcgacgaa ttccacctcg
     9241 gcgagttcat catgatgttc gaaatccaga ccgttttcac cggcaagttg ctgcatatca
     9301 atccattgga ccagcctggc gtggaagccg gcaaaatcgc cacttacgcc ctcatgggaa
     9361 aggccggctt cgacaaggaa cgcgaggaga tcaaacagta tattgaggat agaaaacagc
     9421 agtattagcc agttcgccag acaatttcaa aggagcaaaa aacctgataa acttggagca
     9481 atttattatg ctatcacatt taccttgaca tttgatcggg ggtaggacta ttatcctgtc
     9541 agtatttatc gatgccgttg gcatagtata tagagagttc gaagcgaagt atcagtatga
     9601 tcttcagata gtgttttcag taacttggtt tagaccaatt tcatgaccat ggagtatagt
     9661 gatgaagaaa atcgtattac tgttcgttgt actttccatt ctgttcttaa cgagttgttc
     9721 cataataggc atcaactctg taacttcaaa aacaaaggat atctacggat ctgttataca
     9781 acttccagtg gtagtggatc ttattgttca gaatactaag atcacaaaag agttcactac
     9841 tgaatgtccc cagaatccta ctgatgacgt tatagaagac gttaaaagca tcgcgattgc
     9901 tgctgcactt aaagagcact cggcagatgt acttatcgag cctaattatg atgtgcagat
     9961 tacatcaaag tcagccagta atagtgagat taaggttgta gtcaccggat atcccgctac
    10021 ttataagaat tttcgtaatg cgaccaaagg tgatgtggaa ctaatcgaag gtactacaaa
    10081 tattagaatg cttcccacag
//
LOCUS       DAOOWR010000014         9814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_6899_length_9814_cov_3.329952, whole genome shotgun sequence.
ACCESSION   DAOOWR010000014 DAOOWR010000000
VERSION     DAOOWR010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_9814_cov_3.329952"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1519)
                     /gene="thyX"
                     /locus_tag="PLB85_00865"
     CDS             complement(95..1519)
                     /gene="thyX"
                     /locus_tag="PLB85_00865"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOU71906.1"
                     /translation="MKVTLAGYNIDSSLIRSLDRDSATPEVISAAYARISRSSKSISE
                     LRVEALADVAKARKSNQTIVFDLGHASVAEHAVFNFDVIGVSRLVTESMETLRFASFT
                     EKSQRYVTFSRDYVIPEELSAPAHAKLKARYLRVMDALFAEYQASFEALKTLYTSRFS
                     DLPARAREGMAKEDARYILPLATKTQLGMSINARSLENLLRRLVNDPLNEARELYAEL
                     LKPAKELCPSLVRHVEAEPFTGTFKLPASKVPEDDFPEGESVRLLDSPQDADSVILAA
                     LVYEQTQLSWEQCVEAVSGLSPQARQSLWNKVFAHIRPWSKMHRAFELADFTFELAMS
                     ESCWAQFKRHRVGTIIKQKNPSTGLCVMPEAIISIGRADHWDELLDRVEGLRDSLAAV
                     LPELSAYARLNADRVRVLVKMNLRELYHLIRLRCDEHAQWEINLLARDIASELCQAAP
                     NAASWLCGKSEFVTRASGRPGQND"
     gene            complement(1522..2301)
                     /gene="deoC"
                     /locus_tag="PLB85_00870"
     CDS             complement(1522..2301)
                     /gene="deoC"
                     /locus_tag="PLB85_00870"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU71907.1"
                     /translation="MNQIETIARRLFGDNPPCKCGGAHIVCLGCAVCRAAKTDQIYDP
                     AAGIASIIDATLLRADATQAEVNELCDLANDHKCASVCVNSHFSHPLQLRLSAAVKSC
                     TVINFPLGAGYTYAVAAEALAVINTGIEELDMVQNLSAVKSGHILHSYELIRNIAELC
                     RSNRVLLKVILETCFLSEEEIIACSLYAKKAGAEFIKTSTGFGSAGATVENVRLMRET
                     VGPKIGVKASGGIRTREQALAMIEAGANRIGASSVTALIWG"
     gene            complement(2298..3341)
                     /gene="rlmN"
                     /locus_tag="PLB85_00875"
     CDS             complement(2298..3341)
                     /gene="rlmN"
                     /locus_tag="PLB85_00875"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU71908.1"
                     /translation="MLSSLYGIRPEELQSFFSSAFPAYRVRQLLNWIYRKFVFDPALM
                     TDLASDFRAALVSAFDLSMPAVAEKKLSVDGSAKYRLVLADGAQIEMVLMPQDKKQTL
                     CVSTQVGCSRACAFCATGRMGRKRDLAVHEIVQQVLLAASEAEPALTNLVFMGMGEPL
                     DNLDNVLEAVSLIQHERALAFSPRRVTVSTCGIVPGIQRLAESGLKVKLAVSLNSTRD
                     EVRGQLMPVNNRYPLSVLKRALLYYQHCSPFRITLEYILIPDVNMAAQDLKALRKFCG
                     DLSCKLNFIPYNPVPSLPFRAPTEAEIEAFMESARDLPQAVTLRRSKGADISGACGQL
                     VAESTAKPTGVSP"
     gene            complement(3335..4204)
                     /gene="ricT"
                     /locus_tag="PLB85_00880"
     CDS             complement(3335..4204)
                     /gene="ricT"
                     /locus_tag="PLB85_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOU71909.1"
                     /translation="MAEQKTVAPMREYMEVEFRTGRLGYYQNVQKLPIDPEDLIIVEV
                     ERGEDIAQVTHLSVSGEEISAQIPQTGRVYRIKRLASDIDIEKLRNLPLEEEKAAQTF
                     ADILLRYPFEMKLIETVYQFDGNKLTFFFTADGRIDFRVFVRELANVFRTRIELHQTT
                     GRDEARRLGGYGMCGLQYCCGNFLKRFNQVTIKMAKDQNLAGNLAKISGPCGRLLCCL
                     NFEEDFYLEESKDYPIPGTCVELKGKRLYVFKTDVLNKRIHLSDEDQSLTEVDLESFN
                     KLKVISVPDIEPC"
     gene            complement(4197..5405)
                     /locus_tag="PLB85_00885"
     CDS             complement(4197..5405)
                     /locus_tag="PLB85_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOU71910.1"
                     /translation="MIVFDDQYRAAFFPLAQNRSVGDLRCGILKLRQRLQQFLDDSDE
                     TAVWTDPLLTGLYRERKPEWLENQPALANTLLVNSRLKVTQASIETLKSLPQGSCLHQ
                     GDVILALRTLKEMPALPALDELSGQGYKLLPSELELYCNLCDIIHDNDRLLRFDFEQL
                     FYDNDNYFETEPGVTALNPYGIWIGEKVELKPGVVLDASDGPIVLDQGARVLANAVIT
                     GPAYIGKKSLVRVGAKVYGGTSIGPVCKIGGEVEGSIFQAYSNKQHDGFLGHSFIGEW
                     VNLGADTNNSDLKNNYGNVSFHSYLSDSKVDSGTMFLGTMIGDHAKLGINTSINTGCV
                     IGIGSNLYGPNLISGFIPDFSWGQASQLVRYRFDRFCATAASVKARRGLEFSPAETEL
                     FRVLHGRSGG"
     gene            complement(5424..7481)
                     /locus_tag="PLB85_00890"
     CDS             complement(5424..7481)
                     /locus_tag="PLB85_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOU71911.1"
                     /translation="MTKDNKEKWRKILRIGGIAACVLLGVFIGALWYYQDDLPPTAEL
                     KNYTLNTGSEVYDRSGRMIYLFAFEKRKLVSLKELPPHLIDALIATEDKRFYFHPGVD
                     PIRIVGALVTDIRTGDFSEGASTITQQMARNMFLTLDKTIARKMREIVLALKIEATFS
                     KNEILEIYLNKIFWGSQNHGVETASLYYFNKHARDLNLPESALIIGMIQRPNYYDPFK
                     HPERAKNRRDLVLGRMLKTRKITREQYEEALSTPVNSQRGGGYARFASDYFIEHVRSY
                     LENKYGTERLFEGGLKIYTTLDSEMSSAADSLFNAYLVRVENSSRFPNRYSSVPKDAQ
                     DIDTKYIQGGLALIENKTGYVRVLIGGRSFEHSKFNRMTQARRQPGSSIKPIYYTIAV
                     EKGYHPAKILPDAPITIGNWSPKNSGGTYHGNTRMRVALQWSYNTWAVRCANDIGLPA
                     VRDAFRRFGLKSDPKDLTAALGAYEVTPLDLIAAYTTFPNDGKRAAPVFVTKVEDSNG
                     KLIERIKPQKYNVCSPQVAYLMTSMMQTVAQSGTGGSSRNGYYWDSAGKTGTSNHNND
                     SWFIGFNKAYTLGIWTGFDNRNLKTGTALAASIWGPIMTKAIKIDNNGRTPSANDSRY
                     TFVEPPRIVHLNVNPYTGFVVRGGGIPEIFIEGFEPVVSRDSLSYNFAPSYNFQDQFE
                     QEL"
     gene            complement(7474..8025)
                     /locus_tag="PLB85_00895"
     CDS             complement(7474..8025)
                     /locus_tag="PLB85_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOU71912.1"
                     /translation="MNSTSPSPLTRPKLNPLLMLAFLTATACSIHVFENLIMRLLPLP
                     FLRLGLSNIVVMYLLFEKKTLQASVVAVTKSLVAGAVTFTLLSPATLLSLCGGLAATF
                     VMWAALAANPGFTEYGVSVCGAVAHNVVQLVLVQTVVLPGTQVFVLTPLLLFLGLLSG
                     ILTAWILLRIKDRFLKHKNGKHD"
     gene            complement(8032..8757)
                     /locus_tag="PLB85_00900"
     CDS             complement(8032..8757)
                     /locus_tag="PLB85_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU71913.1"
                     /translation="MIEVEHLTLSLSGQEVLKDVSFRLEDGQNLVIVGRSGSGKTVLI
                     KTLMGLYHPVGGTVKVDDKDVFWDSGNWGSSVRQDFAMVFQSSALLDSYTVFQNVALP
                     LYEREELDYEAIREKVGRSLDVVGLKNIMDKYPAELSGGMRKRVGIARALVYDPRYII
                     FDEPVSGLDPITAGEIIYYITQIIASERATTITITHDTRDLEAIGDQVLFLDEGRVLY
                     YGPLAVLRQAEEPLIRRYLRIGK"
     gene            complement(8744..9148)
                     /locus_tag="PLB85_00905"
     CDS             complement(8744..9148)
                     /locus_tag="PLB85_00905"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU71914.1"
                     /translation="MPEREAGSGPNSQRKRKLSPSVIWPPLLWMALIWLVSSLPGRHL
                     PTPKIISLDKFAHIGIYLILGLLTNRSIQRLGVESRHVWLIYIALLISAGLDEWHQCL
                     IPKRSVSVWDFAANATGLVLAFVAFRISRDRS"
     gene            complement(9152..>9814)
                     /locus_tag="PLB85_00910"
     CDS             complement(9152..>9814)
                     /locus_tag="PLB85_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU71915.1"
                     /translation="REAKLTEIYVPRVRVKAIEVKQSLDQLRDLIVTSGHSRIPVYRE
                     TIDNIVGMIYVKDLLLFPEKKSISELMRPAWFVTENMKVQTLLNQFKIKKMQMAIVVD
                     EYGGTSGIISLEDILEEIVGEIHDEYDRDESPELMQIDAKTWRARGVFNIRQFNQEFN
                     TSIDTDEYDNVAEYLLSQFNHVPAAGETHLLEDTLMFKILESDGKSIKQVEITLLDEE
                     NE"
BASE COUNT         2089 a         2566 c         2934 g         2225 t
ORIGIN      
        1 gcctgacctt caccttcgga gggtgccact ctatccagct gagctacagg gacgcggata
       61 ccgataaaca tcgattttga aaggggggaa tctgtcaatc gttttggccg ggacggccgg
      121 aggcgcgggt gacgaattcg ctcttgccgc aaagccagga agcggcgttg ggagcggcct
      181 ggcaaagttc ggaagcgatg tcgcgggcaa ggaggtttat ctcccactgg gcatgttcgt
      241 cacagcgcag gcggatgagg tggtagagct cgcggaggtt cattttcacc agcaccctga
      301 cgcggtcggc atttaggcgc gcgtatgctg aaagttctgg caggaccgcg gccaggctat
      361 ccctcaggcc ttcaacccgg tccaaaagct cgtcccagtg gtcggctcgg ccaatggaga
      421 taatggcttc aggcattacg cagaggccgg tggaagggtt tttctgcttg ataatggtgc
      481 ccacgcggtg gcgtttgaac tgggcccagc agctttcaga catagccagt tcgaaagtga
      541 aatcagccag ctcgaaggct cggtgcatct tgctccaggg gcggatgtgc gcgaaaacct
      601 tgttccagag actttgtctg gcttgggggg ataatccgga aaccgcttcc acgcactgtt
      661 cccagcttag ttgggtttgt tcatacacaa gagccgcgag gatgacgctg tcggcatcct
      721 gagggctgtc gagcaagcgg acggattccc cttccggaaa atcgtcctcc ggcactttgg
      781 atgccgggag cttaaaagtc cccgtgaaag gttccgcctc cacatggcgg accagggagg
      841 ggcagagttc tttggcaggt ttcaacagct cggcgtaaag ctccctggct tcgttgagcg
      901 ggtcattcac caggcgccgg agcaggtttt ccaagctgcg ggcgttgatg gacatcccaa
      961 gctgggtctt ggtagccagg ggcaggatat aacgggcgtc ttccttggcc atgccctccc
     1021 gggcgcgggc cggcaaatcc gaaaacctcg acgtataaag ggttttcaag gcctcaaaac
     1081 tggcttggta ttcagcgaag agggcgtcca tcacccgcaa atatctggct ttcaatttag
     1141 catgggcagg ggcactcagt tcttcgggaa tcacatagtc ccgggaaaag gtgacgtaac
     1201 gctgcgattt ttcggtgaaa gaggcgaagc gcagggtctc catgctctcg gtgacaaggc
     1261 gggaaacacc gatcacatcg aagttgaaga cagcgtgttc agccacggag gcatggccca
     1321 aatcaaagac gatggtctgg ttcgatttcc tcgctttggc gacatctgcc agcgcttcca
     1381 cccgtaattc cgaaatggat ttgctgctgc ggctgatgcg ggcgtaggcg gcggaaatta
     1441 cttcgggagt ggcggaatca cggtctagag agcggatcag ggagctgtcg atattataac
     1501 cagcgagggt gaccttcaca ttcatcccca gatgagagcg gtgacgctgc tggcgccgat
     1561 gcggttagcg ccggcctcga tcatggccaa agcctgttca cgggtgcgaa tcccgccgga
     1621 ggccttcacg ccgatttttg ggcccaccgt ctcacgcatg aggcggacgt tttccaccgt
     1681 tgccccggcg gagccaaaac cggtggaggt tttgatgaat tccgcacccg ctttcttggc
     1741 gtagagcgag caggcgatga tctcttcctc cgacaggaag caggtttcca ggatcacctt
     1801 gagcagaacc ctattgctgc ggcagagttc agctatgttg cggatgagtt cgtaggaatg
     1861 caggatgtgg ccacttttaa cagcgctgag gttctgcacc atgtccagtt cctcgatgcc
     1921 cgtgttgata acggccaggg cctccgcggc cacggcgtag gtgtagccgg cgcccaaagg
     1981 gaaattaatc acagtgcagg atttaacggc ggcggaaagg cgtagctgga gaggatggga
     2041 aaaatgggaa ttgacgcaga cagaggcgca tttgtggtcg ttggcgaggt cgcaaagctc
     2101 gttcacctca gcttgggtgg cgtcggcgcg gaggagggtg gcgtcaatga tggaggcgat
     2161 tccggcggcg ggatcgtaga tctggtctgt cttggcagcg cggcaaacag cgcagccaag
     2221 acagacgata tgggcgccac cgcatttgca gggcggattg tcgccaaaga gacgccgggc
     2281 gatggtttcg atttggttca tggggaaact cctgttggtt tggcggtgct ctccgcgacc
     2341 agttggccgc aggctccgct gatatccgcg cccttgctgc ggcggagcgt cacggcctgg
     2401 ggcaggtcgc gcgcgctttc catgaaagcc tctatctcgg cttcggtggg ggcccggaac
     2461 ggcaatgagg gcacgggatt ataggggatg aaattaagct tgcaggaaag gtcgccgcag
     2521 aacttgcgca gggctttcag gtcctgggca gccatgttca catccgggat gaggatgtat
     2581 tccagggtta tcctgaaggg gctgcagtgc tggtaataga gcagggctct cttcagaacg
     2641 gacaggggat acctgttgtt caccggcatc agttggccgc gcacttcatc ccgggtggaa
     2701 ttgagcgaca cagccagctt cactttgagg cctgattctg ccaggcgctg gatgccaggc
     2761 acgatcccgc aggtggagac agtcacgcgg cgcgggctga aagctagggc gcgttcgtgc
     2821 tggatgagcg agacagcttc caaaacgttg tccaggttgt ccaaaggctc gcccatgccc
     2881 atgaaaacga ggttggtaag ggcgggctcg gcctcggaag cggccagaag cacctgctgc
     2941 acgatttcgt ggacggcgag atcgcgcttc ctgcccattc tgccggtggc gcagaaggcg
     3001 caggcgcggg aacagccaac ctgggtggaa acacagaggg tctgcttttt atcctggggc
     3061 atcagcacca tctcgatttg ggcgccatcg gccaagacaa ggcggtattt ggcactgccg
     3121 tccacggaaa gctttttctc ggcgacggca ggcatcgaaa ggtcaaaagc cgaaacaagg
     3181 gcggcgcgga aatcagaggc caaatcggtc atcaaggccg ggtcgaaaac gaatttgcgg
     3241 taaatccagt tcagcaactg gcgcacccga taggcaggga aagcggagct gaaaaagctt
     3301 tgcagttctt caggccgaat cccgtacagg ctgctcagca tggctcgata tccggaacgg
     3361 agataacctt gagtttattg aagctctcca gatcgacctc cgtgagactc tgatcctcgt
     3421 ctgaaagatg gatgcgcttg ttcaggacgt cggtcttgaa cacatacaac cgcttgccct
     3481 tcagctccac gcaggttccg gggatgggat aatccttgga ttcctccaga tagaaatcct
     3541 cctcgaaatt gaggcagcag agcagacgcc cgcagggacc cgagattttg gccaggttgc
     3601 cggccaggtt ctggtccttg gccatcttta ttgtaacctg gttgaagcgc ttgaggaaat
     3661 tgccgcagca atactgaagg ccgcacatgc cgtaaccgcc cagacgcctg gcctcatcgc
     3721 gcccggtggt ttggtgaagt tctatgcggg tgcggaaaac gttggcaagc tcgcgcacaa
     3781 agacgcggaa atcaattctg ccatcagcgg tgaagaaaaa ggtcagcttg ttcccgtcga
     3841 actggtaaac ggtctcaatg agcttcattt caaagggata gcgcaagagg atgtccgcga
     3901 aagtttgggc ggctttttcc tcttccaggg gtagattgcg gagtttctcg atgtcgatgt
     3961 cgctggcgag gcgttttatc ctgtaaaccc ggcccgtttg gggaatctgg gcactgatct
     4021 cctctccgga aacgctgagg tgggtaacct gggcgatgtc ctcgccgcgc tccacttcca
     4081 cgataatgag gtcttcaggg tctatgggca gtttttggac gttctgataa taaccaagac
     4141 ggccggtccg gaattccacc tccatgtatt cccgcatggg cgctacagtt ttttgttcag
     4201 ccaccggacc tcccgtgcag aacacggaac agctctgtct ccgcggggct gaattccagt
     4261 ccgcggcggg ctttgaccga ggcagcagtg gcacagaaac ggtcgaagcg gtagcgaacc
     4321 agttgggaag cctgtcccca ggaaaaatcc gggatgaaac ccgaaatcag attcgggccg
     4381 tagaggtttg agccgatgcc gataacgcag cccgtgttga tgctggtgtt aatgcccagt
     4441 ttggcgtgat ctccgatcat ggtgcccagg aacatggtgc cggaatcgac tttggaatcg
     4501 ctgaggtagg aatggaagct cacgtttccg taattgttct taaggtcgct gttattcgtg
     4561 tccgcgccga gattgaccca ttcgccgatg aagctgtggc ccagaaaacc gtcgtgctgt
     4621 ttgtttgaat aggcctggaa gatgctgcct tccacttctc cgccaatctt gcagaccggg
     4681 ccgatggacg tgccaccata gactttggcg ccgacgcgaa ccagggactt tttgccgatg
     4741 taggctgggc cagtgataac agcgttagcc agcacccgag cgccttggtc gagcactata
     4801 gggccgtcgg aggcgtccag caccacaccc ggtttcagtt caaccttttc gcctatccag
     4861 atgccgtagg gattgagtgc ggtgacgcct ggctcggttt caaaatagtt gtcattgtcg
     4921 tagaaaagct gctcgaaatc gaagcgcagc aggcggtcgt tgtcgtggat gatgtcacag
     4981 aggttgcagt aaagttccaa ttcggagggt agcagcttgt aaccctggcc ggaaagctca
     5041 tccagagcag gcagagccgg catctccttc agcgtgcgca gagcaaggat aacgtctcct
     5101 tggtggaggc agcttccctg gggcaggctt ttcagggttt caatggaagc ctgggtcacc
     5161 ttgaggcggg agttcaccag cagggtgttg gctagggcgg gttggttctc cagccactcg
     5221 ggtttgcgct cccggtaaag cccggtgagc agggggtctg tccagacggc ggtttcatcg
     5281 ctgtcatcca gaaactgctg caacctttgg cgcagcttga ggatgccgca gcgcaggtcg
     5341 ccgacactcc ggttttgggc tagggggaag aaagccgccc tgtattggtc gtcaaaaaca
     5401 atcatgttct atactcctca ggattagagt tcctgttcga actggtcctg gaaattgtaa
     5461 ctgggggcga aattgtaaga aagggaatcg cgcgagacaa ccggttcgaa gccctcgatg
     5521 aatatttccg ggattccgcc gcccctcacc acaaaaccgg tgtagggatt gacattcaga
     5581 tgcacgatcc ggggcggctc cacaaaggtg tagcgggaat cgtttgcgct gggcgtgcgg
     5641 ccgttattgt cgatcttgat ggctttggtc atgatcggcc cccaaatgct ggccgcgagg
     5701 gcagttcccg ttttaaggtt gcggttgtca aaaccggtcc agattcccaa ggtataggct
     5761 ttattgaagc cgatgaacca ggaatcgttg ttatggttgc tggtgccggt cttgccggcg
     5821 ctgtcccaat aatagccatt ccgcgaagaa ccacctgtgc ccgattgggc caccgtttgc
     5881 atcatactgg tcatcagata tgccacctgg ggcgagcaaa cattgtattt ttggggtttt
     5941 atccgttcga tgagcttgcc gttcgaatcc tccaccttgg ttacgaagac aggcgctgcc
     6001 cttttgccgt cgtttggaaa tgtggtgtag gcagcgatca ggtcaagcgg ggtcacctca
     6061 taggcgccaa gggcagcggt gagatctttg ggatcggatt tcagaccaaa gcggcggaaa
     6121 gcgtcacgca cggctggaag gccgatatcg ttggcgcagc ggaccgccca ggtgttatat
     6181 gaccactgca gggctacgcg catgcgggta ttgccgtgat aggtgccgcc ggaattcttg
     6241 ggtgaccagt tgccgatggt gatgggggcg tccggcagaa tcttggccgg atggtagccc
     6301 ttttccaccg cgatggtgta ataaatcggt ttgatggaag agcccggctg cctcctggct
     6361 tgggtcatgc ggttgaattt gctgtgctcg aaactgcgcc cgcctatgag caccctcaca
     6421 taaccggttt tgttctcgat cagggccaat cctccctgaa tgtatttggt gtcgatatcc
     6481 tgggcgtctt tgggaaccga ggaatagcgg ttgggaaagc gactgctgtt ttccacccgg
     6541 acgaggtagg cgttgaaaag ggaatccgcg gccgatgaca tctcggaatc cagggtggtg
     6601 taaatcttca gcccgccctc aaagaggcgc tcggtgccgt atttgttttc cagataagag
     6661 cgcacatgct cgatgaagta atccgaagcg aagcgcgcgt agccgcctcc cctctgggaa
     6721 ttgacaggtg tggacagggc ttcctcatac tgttccctcg ttatcttgcg ggttttgagc
     6781 atgcgcccca aaacgaggtc acgcctgttt ttcgcccgtt cgggatgctt gaaggggtcg
     6841 taatagttgg gtcgctggat catgccgatg atgagggccg attcaggcag attcaggtct
     6901 ctggcgtgtt tgttgaaata atacagcgaa gccgtctcca cgccgtgatt ctgcgagccc
     6961 cagaaaatct tgttcaggta tatctccagg atttcgttct tggagaaagt ggcctcgatc
     7021 ttcagggcca gcactatctc gcgcatcttg cgggcaatgg ttttatccag agtaagaaac
     7081 atgttgcggg ccatctgctg ggtgatggtg ctggcgcctt cggagaaatc gcccgtcctg
     7141 atatccgtca ccagggcccc cacgatgcgg atggggtcaa cccccggatg aaagtagaag
     7201 cgtttgtctt ccgtggcgat gagcgcgtcg atcaaatgcg ggggaagttc cttgagggaa
     7261 acaagtttcc gcttctcgaa ggcgaagagg tagatcatcc gtccgctgcg gtcatatacc
     7321 tcgctgccgg tgttcagagt gtaattcttc agctcggccg tgggcggcag atcgtcctgg
     7381 taataccaaa gcgcgccgat aaacactccc agcaaaacgc aagcggcgat tcctccgatc
     7441 cgcaggattt tccgccattt ctctttattg tctttagtca tgcttaccat tcttatgttt
     7501 taaaaaccta tctttaattc gcaacagtat ccaagctgtg aggattccgc tgagcaaacc
     7561 taaaaacaac agcaatggtg tcaacacaaa aacttgcgtg cccggcaaaa ccaccgtttg
     7621 aaccaacacg agttgcacaa cgttgtgcgc cacagccccg caaacgctca ccccgtattc
     7681 cgtgaaaccc gggttcgcgg caagggctgc ccacataacg aaagtggcgg caagccctcc
     7741 gcagagagac agcaaggtgg ctggagacaa aagggtgaag gtcacggctc cggcgaccag
     7801 ggacttggtg acggccacca cagacgcttg cagcgttttt ttttcaaaca gcagatacat
     7861 caccacgata ttggaaaggc ccagccggag aaagggcagc ggcaacaggc gcattatcag
     7921 gttttcaaaa acatggatgc tgcaggcggt cgcggtgaga aaagctaaca tcaacaaggg
     7981 attgagcttg ggtcttgtca atgggctcgg actggtcgaa ttcacaacgt ctcacttccc
     8041 gatccgcaaa taacgcctta tcaggggttc ttcggcttgc ctcagtactg ccagcggccc
     8101 gtaataaagc accctgccct catccagaaa gagaacctgg tccccgatgg cctccaagtc
     8161 acgagtgtcg tgggttatgg ttatggtggt ggccctctcc gaggctatga tttgagtgat
     8221 ataatagatg atttcgccag ccgtgatggg gtccaagccg gaaacgggtt cgtcaaaaat
     8281 gatgtatcgg gggtcgtaaa cgagggcgcg ggcgattccc acccgcttgc gcattccacc
     8341 gctgagttcg gcgggatatt tgtccatgat gtttttcagg ccaaccacat ccaggcttcg
     8401 ccccactttt tccctgatgg cttcgtaatc caattcctcc ctctcataca gaggcagggc
     8461 tacgttctga aaaactgtgt atgaatcaag cagggcagaa ctttggaaga ccatggcgaa
     8521 atcctgccta accgaggaac cccaatttcc agaatcccaa aaaacatctt tatcgtcaac
     8581 cttcacggtg cccccaaccg gatgatagag tcccatcagg gtcttgatca gcacggtttt
     8641 ccccgagccg ctgcgtccca cgatgacaag gttttggccg tcttccagcc tgaagcttac
     8701 atctttcagc acttcctgcc ccgagaggct tagagtgagg tgttcaactt ctatcacgcg
     8761 atatcctgaa agccacgaaa gccaggacaa gtccggtggc gttggccgcg aaatcccaga
     8821 ccgagactga cctttttggg atgaggcatt ggtgccattc gtccagtccg gcggatatca
     8881 gcagggcgat atagataagc caaacgtgcc tggattccac tcccagcctt tgtatggagc
     8941 ggttggtgag caaacccagg atgagatata tgccgatatg ggcaaattta tccagactga
     9001 ttatttttgg agtgggcaga tgcctcccgg gcagcgacga aaccagccag atgagcgcca
     9061 tccaaagtag cggcggccag attacgcttg gggatagttt gcgtttccgc tgggagttcg
     9121 ggccactgcc ggcttctcgt tcaggcatcc gtcactcgtt ttcttcgtcc agcagcgtga
     9181 tttccacctg tttgatgctt ttgccgtcac tctccaagat tttgaacatc agggtatctt
     9241 caagtagatg ggtttccccc gccgcgggaa cgtggttgaa ctgggaaagc agatactccg
     9301 ccacgttgtc atactcgtcg gtgtcgatgg aggtgttgaa ttcctggttg aactggcgga
     9361 tgttgaagac tccccgcgcc cgccaggttt tcgcatcgat ttgcatcagt tcgggcgatt
     9421 cgtcgcggtc gtattcatcg tggatttctc ccacgatttc ctccaggatg tcctcaaggg
     9481 aaatgatgcc tgaggtccct ccgtattcat ccacgacaat ggccatctgc atcttcttga
     9541 ttttgaactg gttgagtagt gtctgcactt tcatgttctc ggtaacgaac caggcgggac
     9601 gcatcagttc gctgatgctt tttttctcgg gaaagagcag caggtccttg acgtagatca
     9661 tccccacgat gttgtctatc gtttcgcggt aaactgggat gcgggaatgg ccgctggtga
     9721 cgatgaggtc acgcagttgg tccagggatt gttttacctc gatagccttg accctgaccc
     9781 gcggcacgta gatttcggtg agcttggctt cccg
//
LOCUS       DAOOWR010000015         9629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7058_length_9629_cov_3.930854, whole genome shotgun sequence.
ACCESSION   DAOOWR010000015 DAOOWR010000000
VERSION     DAOOWR010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7058_length_9629_cov_3.930854"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLB85_00915"
     CDS             complement(<1..362)
                     /locus_tag="PLB85_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU71916.1"
                     /translation="MPENNYTASNIKVMKGLEAVRKRPSMYIGGTSERGLHHLVYEVV
                     DNAIDEAMAGYCDLIKVTITSQGEVEVDDNGRGIPVDEHKDEKISALQVVMTVLHAGG
                     KFDSNIYKVSGGLHGVGVS"
     gene            complement(1082..1864)
                     /locus_tag="PLB85_00920"
     CDS             complement(1082..1864)
                     /locus_tag="PLB85_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU71917.1"
                     /translation="MTKTSSRFWLPLLFSLLLLALWQFLSARLRIAFWVLPSPGAVFG
                     VFRENPALLWSHIRPTLFASVAGLLLATLLGVVTAMAMHSSKPLKQIFYPYLVVSQTV
                     PVVAVAPLVVLWFGYGISAKIFVVVLMCFFPVALGLFDGLRQVSQEQLRLLRSMGASR
                     WQTYRYLKIPASLPAFFTGLKLAATYSVMGAVVGEWLGGQAGLGIYLTRSTKSFRTEQ
                     VFAAILLIILLSLALFGLVSLLDRLLLGWHYKKLDEYEDTSL"
     gene            complement(1861..2808)
                     /gene="murB"
                     /locus_tag="PLB85_00925"
     CDS             complement(1861..2808)
                     /gene="murB"
                     /locus_tag="PLB85_00925"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOU71918.1"
                     /translation="MNKEPELKNQIQSRFPALVEAGAIRFGEPLGPHTSFQIGGPADV
                     FCEPESVTQLIELLDDAVTNGIDYFIMGKGSNLLISDKGMAGMVIHTARLNRIGHDEN
                     FVSAQCGASLKDLCAYAQKLGLAGLEFASGIPGSVGGAVFMNAGAYGGEIKDVLHFSK
                     YLLPEAGHLRSHASVRHLKAGDHGFGYRQSVFQTSGWIHLSSLFRLTPDDPEAIAERM
                     ADLKKQRWEKQPMDLPSGGSAFKRPPGHFAGKLIEDCGLRGFQIGGAAISEKHCGFIV
                     NLGGATAADVLALIEHVQKTVFERFGVMLEPELRLTGRS"
     gene            complement(2805..5648)
                     /locus_tag="PLB85_00930"
     CDS             complement(2805..5648)
                     /locus_tag="PLB85_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOU71919.1"
                     /translation="MEPNDIPPLSDRLNYVAFDTETTGLDPKTDELIELAAVRFRGGE
                     VTERFSTLVRPRGKVPKFIQYLTHIDPQELKDAPDAETALKQFFSFIGDDILVAHNAG
                     FDIGFVNHHSALHGGDLIQHPVWDTVEIARTYLPFTTDHRLGTLTAHFGITLENAHRA
                     GADAEATGLLLARLSEHITSHYSMLSNARLLDLAKQAQLDNSLYNFLRVIVEYQRRYA
                     LIGKKPTPPDVARPNVVENKLPGVNFDIDEVFTPTGLLSQKFPNFEFRAGQVEMAKGI
                     DAAFRGGKHLAVEAGTGVGKSFAYLVPALAFAHRQKTKVVVSTNTKNLQEQLFYKDLP
                     QLKAMLPLPFKAALVKGRENYVCERRWEEFLVEQTRGISPYEAQSLLNLFVWKMLTIS
                     GDVSENSSFDRNRFSTVWRKICSDRYLCAGRKCPHASRCYVMSLRKHVETSSVVVTNH
                     ALLLADLQMENTTLGEYGYLVVDEAHNLMATASRNLGLELSHADLANLFNQLSQSYRR
                     KKTGFLHQLETAMDRSVITDAAKDQIKLYCVNLAEQVTGLRAQALELFQEAQERCSNA
                     DSYGKLRIKDTADFPRLYELLGGLVFAWKDFQKQLTALNNAFSSLNSKQVPNFDALSE
                     TLASYRLRAAETEMGLLSLANPDLENHALWIENSQRVDSKNPGSTLCYAPVDVSQQLN
                     QMLYSTVPSIIFTSATLALRGSFKYFFGQSGLSLVDPEKLDTAIVASPFDYDSQARLI
                     VSSFLPEPRDRFFMNQALGCLEQLLASTDLGTMVLFTSYKDLNTVYDHVSEALYRRKR
                     PFFAQGKAASRSSMLEEFKRHKNAVLLGTNSFWEGVDIQGESLSLLILFKLPFQVPSE
                     PIVEALIDKLDREQKDSFMHFMLPNALLRLRQGFGRLIRSRGDRGIVLVMDSRVSNKY
                     YGQYFKQVLPAKSLEMRSEVELLSEVSSFFGLT"
     gene            complement(5661..>9629)
                     /locus_tag="PLB85_00935"
     CDS             complement(5661..>9629)
                     /locus_tag="PLB85_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOU71920.1"
                     /translation="GRKYDFFYFLTERLNQRKLLKPAALVIDDGDALDPYTLDYLQYL
                     VQYASESGIQIVVLAQNRLFGFSEPELIPPLNVDDLQKLLQLTFPEAEPGFVKESEIL
                     QKISDGNLLVVEHIIAEMKREPKAKFDISPFLDRSYDPLQIYLENVSSLSAAQRELLL
                     AKFILADDATEQRLRALGLCKTLKKDLEALSAAGLLGSAEEGHPVQKKTSLDTWLKQN
                     PEALSGAFYDKLAELLAKDGEHRQTLIRLMMQKRKYNTALFEGLIQELYLLNDNENLL
                     ELYRYQLKNTKKPEKLLAIQQSCAAAHTGLNQMDEAAEHMRQALHICTENSLPAEEIV
                     HMLASNLFAVNSTALALEIIKKYTPDSIDPYWKVKILLLKADILAETEEFETALAELE
                     SVVRQMSAIEDKDLRWTVQGEAKKIKGKTHYYVNEWDQSVEAFKEAETLYNMAKDHKG
                     LAAIYNNLAVLYMFQGDWERSEQYFLRSLKLEQELFNLNGISVCYNNLGSLMDDTGDA
                     NKALQYLEQALKLQKMLNEPYNITNIYNNIGVTLMDHNEYDKAEEALTKSLQTAIDFG
                     FFRNIVATLNNLGALWFKKGDWNQSIALYEQAIKKSEENDFIEGLLRSFTNLGEVYEK
                     QNELNLAFDLCFKGLELLPQVSDDFIKAELYGNLGSVLTKLHRFKEAYSYLVESLDFF
                     KSINARDKIIEGFQKQAYYFILTRNHESADYYLNQALKMAAEQNKPFETGKIHYLRAL
                     LERKNLAAAKQHLHEAIEIFVETVNIYELSLANYELAGVLYDLEEWEKALEILKANKK
                     IIQQYGSIKLLEQNDILTQKISKEYSAQMQDLQFEENLLNQFYEITQKLNTLTDSDVL
                     IEHTLDSLIEISEADGGIFCLHGSETVPDSWDYKLFKNFARDNKYYDVFMNACARVYR
                     SNEIENHKQPRFAPSYNHLVVIPLAIRKDVLGVVLLFSQSGSHYFSERVINLLSALCN
                     QAIVIIENIRSASLEKTHATIREQLHAGNLYANIIGKSPEMMKIFEVIEKVKDTPTTV
                     LLEGPSGTGKELIARALHYGSNRRNKAFVAQYCGALPETLLESELFGHVKGSFTGAAY
                     DKKGLFEIADGGTFFLDEIADISQSTQAKLLRFLQEGEIKRVGATKTEKVNVRVVCAT
                     NASLEEKVRAGDFRLDLYYRLNVIRIEVPPLKRRTGDIPLLAVHFLDKYNTRIGKSVQ
                     GLSDDAMKVLEQYEWPGNVRQLENEIERAVTLVEDGAFIRAQDFSDEVRRYIENQKTI
                     SLLSSRKSLKDAMEELEREMILQAMESTDWNQTQAAKELGISRQGLIQKLKRYNLDRE
                     ES"
BASE COUNT         1917 a         2497 c         2953 g         2262 t
ORIGIN      
        1 gaaacgccca cgccgtggag gccgccggaa accttgtaaa tattgctgtc gaatttgcct
       61 ccggcgtgca ggacggtcat aaccacctgc agggcggaga ttttctcgtc cttgtgttcg
      121 tccaccggga ttccgcggcc gttgtcgtca acctcaacct cgccctggga ggtgatggtc
      181 accttgatca aatcgcagta accggccatg gcctcgtcta tggcgttgtc cacaacctca
      241 tagacgagat ggtgaaggcc gcgctcgctg gtgccgccga tatacatcga ggggcgtttg
      301 cgaacggctt ccaggccttt catcaccttg atgttacttg cagtgtaatt gttttcaggc
      361 ataatctatt gtctcctaat atttagcact gtattcaaac tctcttttgg gttaattggc
      421 gcgccagatt aggacgcgct tccatatttg gttgccataa aatcagatat ggcatttttg
      481 tcaagaacaa tccttctttt ttgggggttt ttatctgctt agtttacagc atgttacaaa
      541 ccgagttcgc cagccgggga accgacttgc cgggcttcgc atcctcctga gcgagaaaat
      601 ccctcccgaa aagccccttt ttgggccgcc tggcgaatta taaagccaga tacataagct
      661 tggattcttt cccttgaatg ggacagcgta ttcatgtcaa acatccatat tggcgatttt
      721 cagcctcttt tcaggggttt cttgtgaaga aggtgcaaag gctgtttgaa aagtcagccg
      781 aaagagagcc ataacttttt gcccgccgcg atgccctgcc agtcacaccc ttagcgcccg
      841 cggccatccc tctcccccgc ctcccgcgtg atcctcgcat caaatgcggg cattatacgt
      901 gaggcgtgtc ttggacacag taaagggcct taacggccaa agggcattgc caggcacacg
      961 ctggcgacgg gcaagggata tgaatcgaag gcagcagata atcctgtcgt ccatgctcat
     1021 tgtgttagct gtacgccagg ttacccgggt tgaaaatgag taagaggcgg cggaaggccc
     1081 ttcagaggct cgtgtcttcg tattcgtcga gttttttata gtgccagccc agcagaagcc
     1141 ggtcgaggag ggagacgagg ccgaagaggg cgaggctgag caggatgatg aggaggatag
     1201 cggcaaagac ctgctcggtg cggaaagatt tggtgctgcg ggttagatag atacccaggc
     1261 cggcctgacc gccaagccat tctccaacca cagcccccat cacgctatag gtggcggcga
     1321 gcttcaaccc ggtgaaaaaa gccggcagcg aggccggaat cttcagataa cgataggttt
     1381 gccaacggct cgcgcccatc gaacgcagca ggcgaagctg ttcctgggaa acctgcctga
     1441 gtccgtcgaa gaggcccagc gccacgggga aaaagcacat cagcaccacc acgaagattt
     1501 tggccgaaat gccgtaaccg aaccagagca cgaccagcgg cgccactgca accacgggca
     1561 cggtttggga aacaacaaga taggggtaga aaatctgttt cagcggtttg gaactgtgca
     1621 tggccatcgc cgtcaccacg cccagaaggg tggccagaag caggccggcg acgctggcga
     1681 agagggtggg ccggatatga gaccagagca gggcaggatt ttcccggaac acgccaaaca
     1741 ccgccccggg tgagggcaac acccagaaag cgatccggag gcgggcgctg agaaactgcc
     1801 acagcgcgag cagcagcaga gaaaaaagca gcggcagcca gaaacgggag gaggttttgg
     1861 tcatgacctg ccggtgaggc gcagttccgg ctccagcatc acgccgaagc gctcgaaaac
     1921 ggtcttttgg acgtgttcga tgagagcaag aacatccgcg gcggtggctc cgcccagatt
     1981 gacaatgaag ccacaatgct tctcggagat ggcagcgccg ccgatctgaa agccgcgcag
     2041 tccgcaatcc tcaatcagct tgcccgcgaa atggccaggg gggcgcttga acgccgaccc
     2101 cccgctgggc aggtccatcg gctgcttttc ccaacgctgt tttttcaaat ccgccatgcg
     2161 ttccgctatg gcctccgggt cgtcaggagt gaggcggaaa aggctgctga ggtggatcca
     2221 gccgctggtt tgaaagacgc tctggcggta gccgaagccg tggtcccctg ccttgaggtg
     2281 gcgcaccgaa gcgtgggagc gcagatgccc cgcttccggc agcagatact tgctgaagtg
     2341 gagcacatcc ttgatctcac caccataggc gccggcgttc atgaaaacag cgccacccac
     2401 gctgccgggt atgccggagg cgaattccag ccccgccagg ccaagcttct gggcataggc
     2461 gcagaggtct ttgaggctgg ctccgcactg tgcgctcacg aagttttcgt catggccgat
     2521 gcggttgagg cgggcggtgt ggataaccat gcctgccatg cccttgtcgc ttatcaataa
     2581 attggatccc ttgcccatga taaagtaatc tatgccattg gtgacagcat catcaagcag
     2641 ctcaatcagt tgggtgacgc tttcgggttc gcagaagaca tccgccggcc cgccgatttg
     2701 gaagctggtg tgcgggccaa gaggttcgcc gaagcggatg gcgccagcct caacgagggc
     2761 cgggaagcgg gattggatct ggtttttcag ttcaggctcc ttgttcatgt aaggccaaaa
     2821 aagctgctaa cctcgctgag gagttccacc tcgcttctca tctccaggga tttggcgggc
     2881 agcacctgtt tgaagtattg gccgtagtat ttgttggaca cgcgtgaatc catcaccagc
     2941 acaatgccgc ggtcgccccg gctgcggatg aggcgtccga aaccctggcg cagcctcagc
     3001 agggcgttgg gcagcatgaa gtgcatgaag gaatccttct gttcgcggtc gagtttgtcg
     3061 atcagtgcct ccacaatcgg ttcggacggc acttggaaag ggagtttgaa gaggatgagc
     3121 agggaaaggg attcgccttg gatgtccact ccctcccaaa aggaattggt gcccaggagg
     3181 acggcgtttt tgtggcgctt gaattcctcg agcatggagc tacggctagc agccttgccc
     3241 tgggcgaaga aggggcgttt gcggcggtag agggcttcgc taacgtggtc gtaaacggtg
     3301 ttcaggtctt tgtaactggt gaaaagcacc atggtgccga ggtcggtgga agccagcaac
     3361 tgctcgaggc agcccaaagc ctggttcatg aaaaagcggt ccctaggttc cggcaggaag
     3421 ctgctcacga tgaggcgggc ctggctgtca tagtcgaaag gcgaagccac gatggcggtg
     3481 tccagctttt ccgggtcaac caggctgagc ccgctctggc cgaagaaata cttgaaggag
     3541 cctcgcaaag cgagggtggc ggaggtgaaa atgatgctgg gcacggtgga atagagcatc
     3601 tggttgagct gttgggaaac gtccaccggc gcgtagcaga gagtggaacc gggatttttt
     3661 gagtccacgc gctgggagtt ttctatccag agggcgtggt tttccaggtc cggattggcc
     3721 aggctgagga ggcccatttc cgtttcagcc gcgcgcaggc ggtaagaagc caaagtttcg
     3781 gagagggcat cgaaattcgg cacttgcttg ctgttgaggc tggaaaaggc gttgttaagt
     3841 gcagtgagct gcttctgaaa gtctttccag gcgaaaacca gcccgcccag cagttcgtag
     3901 agccggggaa agtcggcagt gtccttgatg cgcagtttgc cgtaggaatc ggcattgctg
     3961 cagcgttcct gggcctcctg aaagagttcc agcgcctgag cacgcaagcc ggtgacctgc
     4021 tccgccagat tcacgcagta aagcttgatc tggtctttgg cggcgtcggt gatgacgctc
     4081 ctgtccatgg cggtttccaa ctgatggagg aagccggttt ttttgcggcg gtaagattgg
     4141 gaaagctggt tgaagaggtt ggctagatcg gcgtgggaaa gctccaggcc gaggttgcgc
     4201 gaggcggtgg ccatcaggtt gtgagcctca tccaccacca ggtatccgta ttcgccgagg
     4261 gtggtgtttt ccatctgaag gtcggccaat agcagggcat ggttggtgac cacaacggag
     4321 gaggtctcga cgtgtttgcg caggctcatc acatagcatc tggaggcgtg ggggcatttg
     4381 cgtcccgcgc agagataacg atcggaacag atcttgcgcc agactgtgct gaagcggttg
     4441 cggtcgaaag aggaattctc gctgacatcg ccggagatgg tgagcatttt ccagacgaag
     4501 aggttgagca gggactgggc ttcatagggc gaaatccccc tcgtctgctc caccaggaat
     4561 tcctcccagc gcctttcgca gacatagttt tcgcgcccct tcaccagggc ggctttgaag
     4621 ggcaggggca gcatggcttt cagttggggc aggtccttat aaaaaagctg ttcctggagg
     4681 ttcttggtat tggtggaaac gaccaccttg gtcttttggc ggtgggcaaa ggcgagggcg
     4741 ggaaccaggt aggcaaagga ttttcccacc ccggtgccgg cttccacggc gagatgtttg
     4801 cctccgcgga aggcggcgtc gatccctttg gccatttcca cctggccggc gcggaactcg
     4861 aaattgggaa acttttggga gagaaggccg gtgggggtga aaacctcatc gatgtcgaaa
     4921 ttcacgcccg ggagcttgtt ctcaaccacg ttcggcctcg ccacgtccgg cggagtgggc
     4981 tttttgccga tgagggcgta gcggcgctga tactcaacta tgacgcgcag gaagttgtat
     5041 agcgagttgt caagctgggc ctgtttggcc aggtccagca ggcgggcgtt cgatagcatg
     5101 gaatagtggc tggtgatatg ttcagacagc cttgccagca gcagcccggt ggcttcggcg
     5161 tccgccccag cgcggtgggc gttttccagg gtgatgccaa aatgggcggt cagggtcccc
     5221 aaacgatggt cggtggtgaa gggcaggtag gtcctggcga tttccaccgt gtcccagacg
     5281 gggtgctgga taaggtcccc accgtggagg gcggaatggt ggttcacgaa gccgatgtca
     5341 aagccggcgt tgtgggccac aaggatatcg tcgccgatga aggaaaagaa ctgcttcagg
     5401 gctgtttccg cgtccggagc gtccttcagc tcctgggggt cgatgtgggt taggtattgg
     5461 atgaatttgg gcacctttcc gcgcggacgc accaaggtgg aaaagcgctc cgtcacctcg
     5521 ccaccgcgaa agcggactgc agccagctcg atcagttcgt cggttttggg gtccagaccc
     5581 gtggtttcgg tgtcgaaagc cacatagttc agtctgtcgc tcaggggcgg gatgtcattg
     5641 ggttccaaat gtcctccgga tcagctttct tccctgtcca aattgtatcg tttgagtttc
     5701 tggataagcc cctggcgcga gatgcccagt tccttggcgg cctgggtctg attccagtcc
     5761 gtggattcca tggcttgcag gatcatctcg cgctccagct cttccatcgc gtctttgagc
     5821 gatttgcggc tggagagcag actgatggtt ttctggtttt caatgtaacg gcgcacctca
     5881 tccgagaaat cctgcgcccg gatgaaagcg ccgtcctcaa ccagggtcac ggcccgctcg
     5941 atttcgttct ccaactggcg cacgttgccg ggccattcat actgttccag cactttcatg
     6001 gcgtcgtcgg agaggccttg aaccgatttg ccgatgcggg tgttgtattt atccaggaaa
     6061 tggacggcca gcaggggaat gtcgccggtg cggcgcttca ggggcggaac ctcgatgcgg
     6121 atcacgttca ggcggtaata gaggtcgagg cggaaatcgc cggcacgcac cttttcctcc
     6181 agggaagcgt ttgtggcaca gaccacgcgg acgtttacct tttcggtctt ggtggcgccc
     6241 acgcgcttga tttcgccttc ctggaggaag cggagcagct tggcctgggt gctttggctg
     6301 atgtcggcga tttcgtccag gaaaaaggta cccccgtcgg caatctcgaa gaggcccttt
     6361 ttatcgtagg ccgcgccggt gaaggagcct ttaacgtggc cgaagagttc gctttccagc
     6421 agagtttccg gcaaagcgcc gcagtattgg gccacgaaag ccttgttgcg cctgttgctg
     6481 ccgtagtgca aggcccgcgc tatcagttcc ttgcccgttc cgctggggcc ttccagcagt
     6541 accgtggtgg gggtgtcctt caccttttca attacctcga agatcttcat catttccggg
     6601 cttttgccga tgatgttggc gtagaggttg ccggcgtgaa gttgttcgcg gatggtggcg
     6661 tgggtctttt ccaagctggc ggagcggatg ttttctatga tcacgatagc ctggttgcag
     6721 agcgcgctga gcaggttgat gacgcgctcg gagaaatagt gggagccgct ctggctgaag
     6781 agcagcacca cgcccaaaac gtccttgcga atagccaggg gaatcaccac cagatggttg
     6841 taggaaggcg cgaagcgagg ctgcttgtgg ttttcgatct cgttggagcg gtaaacccgc
     6901 gcacaggcat tcataaagac gtcgtaatac ttgttgtccc tggcgaaatt cttgaacagc
     6961 ttgtaatccc aggaatccgg aaccgtctcg cttccgtgga ggcagaagat gcccccgtca
     7021 gcctcggaaa tctcaatcag gctgtccagg gtgtgctcta tgagcacgtc tgagtcggtg
     7081 agtgtgttca gcttctgggt aatttcgtag aactggttga gcaggttttc ttcgaactgc
     7141 aggtcctgca tctgggctga gtattccttg gaaatcttct gcgtgaggat gtcgttttgt
     7201 tccaggagtt tgatggagcc gtactgctgt atgatcttct tgttggcctt gaggatttcc
     7261 agcgcttttt cccattcctc gaggtcgtaa agcaccccgg ccagttcata atttgccagc
     7321 gaaagctcgt agatattgac cgtttccaca aaaatctcaa tggcctcatg caggtgctgc
     7381 ttggcggcag ccaggttttt gcgctcaagc agagcgcgca ggtagtggat tttgcctgtt
     7441 tcaaagggtt tgttttgctc cgcggccatc tttagggctt ggttcagata atagtccgcg
     7501 ctttcgtggt tgcgggtgag aatgaagtag taggcttgct tctggaagcc ttcgatgatt
     7561 ttgtcgcggg cgttgatgga tttgaagaag tccaggcttt ccaccaggta ggaataggct
     7621 tccttgaagc ggtggagctt ggttagcacg ctgccgaggt tgccgtaaag ctcggccttg
     7681 atgaaatcat cgctcacctg aggcagcagt tcgaggcctt tgaaacagag gtcgaaggcc
     7741 agattcagct cgttctgctt ttcgtaaacc tcgcccagat tggtgaaaga gcgcagcagc
     7801 ccctcgatga aatcgttctc ctcgcttttc ttgatggcct gttcgtaaag ggcgatggat
     7861 tggttccagt cccctttctt gaaccagagg gcgccaaggt tgttcagcgt ggccacgatg
     7921 ttgcggaaaa aaccgaagtc gatggcggtc tgcagcgatt tggtgagggc ttcctcggcc
     7981 ttgtcatatt cgttgtggtc catcagcgtg acgccgatgt tgttgtagat gttggtgatg
     8041 ttgtagggct cgttgagcat tttttgcagt ttcagggctt gttccagata ctgcagggct
     8101 ttgttggcgt cgccagtgtc gtccatcagg ctgcccaggt tattgtagca gacgctgatg
     8161 ccgttcaggt tgaatagttc ctgctccagt ttcaggctgc gcaggaaata ctgttcgctg
     8221 cgttcccaat ctccctggaa catgtaaagc acggccaggt tgttgtagat ggcggccagg
     8281 cctttgtggt ccttcgccat gttatacagg gtctctgctt ctttgaaggc ctccacgctc
     8341 tggtcccatt cattcacgta atagtgggtt ttgcccttga tctttttagc ctcgccctga
     8401 accgtccagc ggagatcctt gtcctcgatg gcgctcatct ggcgcaccac gctttccagt
     8461 tccgccaggg ctgtctcgaa ttcctcggtt tcagccagga tatccgcctt cagcagcagg
     8521 attttcacct tccagtaagg gtcgatggag tccggggtgt attttttgat gatttccaga
     8581 gccagggcgg tgctgttcac ggcgaagagg ttagaggcca gcatgtgcac gatttcttcc
     8641 gctggaagcg agttttccgt gcagatgtgc agggcctggc gcatatgctc agccgcctcg
     8701 tccatctggt ttaggccggt gtgggcggcg gcgcagctct gctggatggc cagcagcttc
     8761 tccggctttt tggtgttttt cagttgatag cggtaaagct ccagcaggtt ctcgttgtcg
     8821 ttgagcaggt agagttcttg aatcagtcct tcgaacagcg cggtgttgta tttgcgtttt
     8881 tgcatcatca gccggataag cgtctgacgg tgttcgccgt cctttgccag cagctccgcg
     8941 agtttatcgt aaaaagctcc ggagagggct tcgggattct gcttcagcca ggtgtccagg
     9001 ctggtttttt tctgcacggg atggccttct tccgcgcttc ccagcaatcc cgccgcgctc
     9061 agcgcttcca ggtccttctt cagcgttttg cacaggccca gcgccctgag ccgctgctcc
     9121 gtggcgtcat ctgccaggat gaacttcgcc agcagcagtt cgcgctgcgc ggcgctgagg
     9181 ctggaaacat tctccagata tatctgcagg ggatcgtaac tgcggtccag aaaagggctg
     9241 atgtcaaatt tggctttggg ttcgcgcttc atttcagcga taatgtgttc caccaccagc
     9301 agattcccgt cggagatctt ttgcaggatt tcgctttcct taacaaagcc cggttccgct
     9361 tccggaaagg taagctgtag aagcttttgc aggtcgtcaa cattcagcgg gggaatgagt
     9421 tccggctccg aaaagccaaa gaggcggttt tgagccagca caactatttg gatgccgctt
     9481 tcggaggcgt attgcaccag atactgcaga taatcgaggg tgtaggggtc gagggcgtcg
     9541 ccatcgtcga tcaccagggc ggcgggtttc agaagttttc tttggttcag ccgttcggtg
     9601 aggaaataga aaaagtcata tttgcggcc
//
LOCUS       DAOOWR010000016         9601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7085_length_9601_cov_3.995495, whole genome shotgun sequence.
ACCESSION   DAOOWR010000016 DAOOWR010000000
VERSION     DAOOWR010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_9601_cov_3.995495"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1688)
                     /locus_tag="PLB85_00940"
     CDS             complement(153..1688)
                     /locus_tag="PLB85_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU71921.1"
                     /translation="MKNSTSSGISRKKTDNFKALRLYLIMGLLLIFTAFAVYAQILIK
                     NAKREQEYVPRIFAQYIAYTDSYLRQSEKYTQMLAEITSKRFELLQEIDFQEAISDYM
                     FLDFPGKNPIPVIITDADTIPQYWNQVQVSSDTSYEDLSEADRLLLRQEMERMNRSEL
                     IEGGKVINYVYIARPVSLQDFIKGIDYSVVVTDREKRPLYWRNVDIAEDLSWDQTPVE
                     ARKLLRDKMSGMTEVPLAQASEQLGYIYFTTPKSLSRIGSLVILELLLLILILAFGAY
                     GLILLHRTEKDTLWIGLAKETSHQFATPITSLLGWLERLRSTPPGAMSQEEYDKVLDQ
                     MTTDLNLLSYNANRFGKVGSQTNLKPVELHALLEESVSYFQARMPHLASRIDIYLISK
                     IQGVQVMLDKDLFKWALENLIKNCVDAMSKKGGNIFITATQNKKHVYVHIRDEGKGIA
                     HSQWKKIFEPGVTTKTRGWGLGLSLAKRIIEEYHKGHIRVLESTLNEGTTFEIKLVKE
                     LYK"
     gene            complement(1642..2412)
                     /gene="mazG"
                     /locus_tag="PLB85_00945"
     CDS             complement(1642..2412)
                     /gene="mazG"
                     /locus_tag="PLB85_00945"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU71922.1"
                     /translation="MKEFQDLVDIIAQLREPLSGCPWDIKQTSQSLVPNFIEELYEAV
                     EAIEDGDETGLMEELGDLMLHIVFQAQIATEEQRFDITDVLRAINDKLVRRHPHVFGQ
                     LEVNDADNVKMNWERLKKSEKKDRKSVLEGIPRALPALIHAQRSQEKAASVGFDWPNL
                     KPVLAKLDEERKELDEALANEDHLQIKEELGDMLFTLVNLARKLHIDAESALKETSRK
                     FHKRFNFIEEHYRKNGDDIHEASLEELDLLWDIAKEDR"
     gene            complement(2409..3692)
                     /locus_tag="PLB85_00950"
     CDS             complement(2409..3692)
                     /locus_tag="PLB85_00950"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOU71923.1"
                     /translation="MILKRLAPSIAYLGTAALVLAAAAAAQGMELPQFGFIGAFLGAW
                     LLTVLLFGVSMRNSLLFSTLFFIKPLPTAFVSVLFILLFTLLLEFQRGKLKQLIIPYP
                     VILAVLMGTAIYGIARARSDWYAYLYFLSTAVLPAIAMLIAANSSARKTDYMLWLKAI
                     ALIGTFLAVIGVPMAILNPGERCGSLWITAMTINGFYLLAFFFSIALGVKAGKTSERY
                     LWYVCALLVLLGMLYTYTRVTLVAVFAGFFLLMLKMKRMRLFGMGLVLLVPLIIPSSM
                     VSRIELGLTFDYSIFIRFLAWYYSLQQIASHPWFGIGIDVWKDWYAGAVPMDMLYAEH
                     SHNLPLKIWLELGVFGFISYFYVIVAVLRKYYLRVVKPDAGNFHRIVLIGVIALLIAC
                     LTDIFIQQYSVALAFWITLSLMYMLSRARGINEEQ"
     gene            complement(3706..4869)
                     /locus_tag="PLB85_00955"
     CDS             complement(3706..4869)
                     /locus_tag="PLB85_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOU71924.1"
                     /translation="MDKKEFDFFELLRIVIKNRRFIIIFVAVVSIGAVIYSLLTPQIW
                     ESSASFYVVGDQASALPFNIEGLSGLTSKLLGSDNAQNAINSVSAMRSRGFSEDVIRR
                     FHLIEYYKITEKDSLKAMDLALKKLRKKTMSIDYSTDTGLVSVSAETKRKNLSRDMVD
                     YYLQKLEIYNKEQKLTKGKMNREFLEGRVRETEAEIDSLLYAVKDFQTRHNAIDIETQ
                     TASLISSYSDVIAAKMQTDIELELARKNYAPDSPIVAELKSRSDALGRQIRDLEAGSG
                     ALKPRYLIDIDSLPNLGTQFAQLKMNLEIQLKVFEFLYPQFEAARLEELRDMPTLDIL
                     DTPREAGMRVRPKRAMLCLIAFGLALVAAVVLTLIKNVFDQNKERLQEIKKEL"
     gene            complement(4902..6170)
                     /gene="serS"
                     /locus_tag="PLB85_00960"
     CDS             complement(4902..6170)
                     /gene="serS"
                     /locus_tag="PLB85_00960"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU71925.1"
                     /translation="MLDIKFIRANPELVRNAILNKNEKADLDALLKSDELRRKLQFDF
                     DQLKTRQNQVSQTIAQKKKAKEDATDLLQEMSAVADEIKTLNTALNEANARLENLLLQ
                     VPNVPHPSVPVGSDESANKIVREWGSKPCFDFEPLDHLEIAERNRLLDLPRGAKLSGS
                     GFPVYTGRGAALERALINFMLNIHIQEHGYTEVSVPVLVNRKTMTGTGQLPKLEDDMY
                     RVDLDDLFLIPTAEVPVTNLHADEVLRQDQLPLKYVSYTPCFRREAGSYGKDTRGLQR
                     LHQFNKVEMVRLVEPESSYNALEEMLLDAERILQALGLHYRVVQLCTGDLSFASAKTY
                     DLEVWAPGVGKYLEVSSVSNFDDFQARRANIRYRDKDGKPRFLHTLNGSGLATPRLFI
                     ALLENYQNATGGVDLPTPLTGYLAQFGGAS"
     gene            complement(6173..6952)
                     /gene="tpiA"
                     /locus_tag="PLB85_00965"
     CDS             complement(6173..6952)
                     /gene="tpiA"
                     /locus_tag="PLB85_00965"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU71926.1"
                     /translation="MRKIIIAGNWKMNKDASETEHFCRELASYLKEHDAERVLPLVAP
                     VHTFLAQARNILRDLPASIAAQDVSSHNEGAYTGEVSATQLSSLGITHCIIGHSERRQ
                     YHAETDALIREKLLRLRENGIAPIVCIGETLEQREQGETEQVILKQLEGCFEQIDMPA
                     GKETVIAYEPVWAIGTGKTASAEQAQEVHALIRAWLQNRYGLETAQNIHILYGGSVKP
                     ENLRGLLRQEDIDGGLIGGASLQVESFQAMIGIAVEVSQGY"
     gene            complement(7144..7977)
                     /locus_tag="PLB85_00970"
     CDS             complement(7144..7977)
                     /locus_tag="PLB85_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71927.1"
                     /translation="MIVLLTGSMGFLVAESPLPSMVRYRSTLNFEHEYPQAWLRLDRS
                     NTEAVRSELLPAGADSLSLANSHTNFVLNQIDSLVIKAAFMESSGEEGPGLVMVNFGP
                     KIDRFDDDAMNLLEKRFQTMLLPPGALVKTLETSYVRQGLKRMLVLTASVTNPGQPGV
                     WSYYSLTVNGEGRSFTFTLRGKETTIQRLQPVLSEMTGSLVDNFNVTRSISRIPRWSE
                     IVLILLFSLLISGLIIRLRDQVIVASRHKRRGITFFQLFLIVAAVLVGVSHLLTSCQA
                     G"
     gene            complement(8176..>9601)
                     /locus_tag="PLB85_00975"
     CDS             complement(8176..>9601)
                     /locus_tag="PLB85_00975"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU71928.1"
                     /translation="NLSGKSSIVAIREPSLGPVFGIKGGAAGGGWSQVLPMEDINLHF
                     TGDFHAITTANNTLAALVDNYIYFNNELNLDPRRITWKRVLDVNDRMLRKIVIGLGGS
                     KQGFPREDGFDITPASEIMAILCLSNNMEELKEKIGNITVGFTFDGEPVHVNQLGYEG
                     AIAALLKDALQPNLVQTTENTPAFVHGGPFANIAQGANSIIATKAALRLSEYVITEAG
                     FGFDLGAEKFFDLVCPYGKLCTDAVVLVATIRALKLHGGKRLKDINEPDPKAVEAGLE
                     NLAKHLENIDKFGMKSIVAINRFPNDTDEEIGLVSDFVASKGFESSVVNYHAEGGKGG
                     LELAEQVIGMVDNEICHYHGLYDWDSPVKDKIFTIASQIYGAQNVDYTMEAKADLKRI
                     NKYGYDKLPICIAKTQKSLSDNPELIGRPKDFLVTVRRIVISSGAGFLVPITGEIMRM
                     PGLPKHPAAETIDVEEDGDIRGLF"
BASE COUNT         2129 a         2576 c         2486 g         2410 t
ORIGIN      
        1 cagctgcttt ggatagtggt tggcaatgat ttcggcgcag gctttgccag ctgtttccat
       61 caacaaccgg gaaggaaggc caaagtcttt gatggcgcga ttgtccaagt ctttcatctg
      121 ggtggaacta agcagcgggc gcatattaac tcctatttgt aaagttcttt tacaagtttg
      181 atttcgaagg ttgtgccttc attcagagtg ctttccagca cacggatgtg acctttatga
      241 tattcttcga tgatgcgttt ggccaggctg agtcccagac cccaacctcg ggttttggtg
      301 gtcacgccgg gttcgaagat ttttttccac tgggagtggg caatgccctt gccctcatcg
      361 cgaatatgca cgtaaacatg ttttttgttc tgggtggcag ttataaagat gtttccgcct
      421 tttttggaca tggcatccac gcagtttttg atcaggtttt ccaaagccca tttgaacagg
      481 tctttgtcca gcatcacctg tacaccttgg atcttggaaa tcagatagat gtcgatgcgg
      541 gaggccaaat ggggcatacg ggcctgaaaa taggaaacgc tttcttcaag caaagcgtgg
      601 agttccaccg gcttgagatt ggtctgcgag cccaccttgc caaaacggtt ggcattgtag
      661 ctcagcaggt tgaggtccgt ggtcatctgg tccagaacct tatcgtattc ttcctggctc
      721 atggcgccgg gaggtgtgct gcgcaggcgt tccagccagc ccagtagcga tgttatcgga
      781 gtggcgaact gatgtgaggt ttccttggcc agtccgatcc aaagggtatc cttctccgtg
      841 cggtgcagca gaatcagccc ataggctcca aaagccagga tcaatataag cagaagcagt
      901 tccaggatca ccagggagcc gatacgggac aatgattttg gcgttgtgaa atagatatat
      961 cccaactgtt cagaggcttg ggcaagcggc acctcggtca tcccggacat tttatctctt
     1021 agtagtttcc gggcttcgac cggcgtctga tcccagctta gatcttcagc tatgtccaca
     1081 ttgcgccagt agagtggcct tttctcacgg tcagttacaa ccaccgaata gtcgatccct
     1141 ttgatgaaat cctgaagtga cacaggccgc gcgatataaa catagttgat cactttgccg
     1201 ccttcgatca gttcactgcg gttcatgcgc tccatttcct gtctaagcag cagcctgtca
     1261 gcttcgctaa ggtcctcgta gcttgtatcg gaagagacct gcacctggtt ccaatactgg
     1321 ggaatggtat cggcgtcggt gatgatcacc gggatggggt ttttgccggg gaaatccagg
     1381 aacatataat cgctgatggc ttcctggaaa tcgatctctt gcagcagttc aaagcgtttg
     1441 gaagtgatct ctgccagcat ctgggtgtat ttctcggatt gcctcaagta gctgtctgta
     1501 taagcgatgt actgggcaaa aatgcggggt acatattctt gttcgcgttt ggcgtttttg
     1561 atcaggatct gggcatacac ggcaaaagcc gtgaaaatca gcagcagccc catgatcaga
     1621 tagaggcgca gggccttgaa attatctgtc ttctttcgcg atatcccaga ggaggtcgag
     1681 ttcttcaagg cttgcctcat gaatgtcgtc tccgtttttc cggtagtgct cttcaatgaa
     1741 gttgaaacgt ttgtggaatt tcctggaagt ttccttcaaa gcgctctcgg catcgatatg
     1801 cagcttgcga gcgaggttca ccagcgtaaa cagcatgtct cccaattctt ccttgatctg
     1861 tagatgatcc tcgttggcca gagcttcgtc cagttccttg cgctcctcat ccagctttgc
     1921 caatacgggt ttcagattcg gccagtcgaa accaaccgaa gccgcctttt cctgtgaacg
     1981 ctgggcgtgg atgagcgctg gcagcgccct gggaattcct tccaaaacgc tcttgcggtc
     2041 tttcttttca cttttcttca agcgttccca gttcatcttt acgttgtccg catcgttaac
     2101 ctcaagctgc ccaaacacat gtggatgccg ccgcaccagc ttgtcattta tggcacgcag
     2161 cacgtcggtg atgtcgaacc gctgttcctc agtcgctatt tgggcctgaa aaactatgtg
     2221 cagcatcaag tcgcccagct cttccatcaa gcctgtctcg tctccatcct cgattgcctc
     2281 cacagcttcg tataactcct cgatgaagtt tggcaccagg ctttgagagg tttgtttgat
     2341 gtcccaggga cagccggaca gaggttcgcg cagctgcgcg atgatatcca ccaaatcttg
     2401 aaattctttc attgttcctc gtttataccc ctggcgcggg agagcatgta catcaagctt
     2461 agcgtgatcc aaaaggccag cgccaccgag tattgctgaa tgaagatatc ggtaaggcag
     2521 gcgatcagca gggctatgac accaatcagt acaatgcggt ggaagttgcc cgcgtccggt
     2581 ttcaccaccc tcaaataata cttgcgtaaa accgccacga tcacgtaaaa gtaacttatg
     2641 aaaccaaata cacccagttc cagccagatc ttgagcggca ggttgtggga atgttcagcg
     2701 tagagcatgt ccatcggtac ggcgccggcg taccagtctt tccagacgtc aattccaatc
     2761 ccgaaccagg gatggcttgc gatttgctgt agtgagtaat accaagccag aaagcggatg
     2821 aagatcgaat agtcgaaagt cagtcccagt tcgatccggc ttaccattga agacggaatg
     2881 attagtggca ccagcaatac aagacccatc ccaaacagcc gcatccgctt cattttaagc
     2941 atcagcaaaa agaacccggc aaacacagcc accagagtga cgcgcgtgta ggtgtagagc
     3001 atgcccagca gtaccaacag agcgcaaaca taccatagat agcgttccga cgtcttaccg
     3061 gccttcactc ccagggcgat gctgaagaag aaggccagaa ggtaaaaacc gttgatggtc
     3121 atagccgtga tccaaagcga tccgcagcgt tcacccggat tgaggatggc catcggcaca
     3181 cctatcaccg ccagaaaggt cccgatcagc gctatcgcct tcagccacag catgtaatcc
     3241 gtcttccggg cgctgctatt ggccgcgatc agcatggcga tagccggcaa tactgcagtg
     3301 gacaggaaat aaagatatgc gtaccaatca ctccgggctc tggcgattcc gtaaatagca
     3361 gtgcccatca gcactgccag tatcacggga taagggataa tgagctgctt cagctttccg
     3421 cgttggaatt ccagcagcag tgtgaacagc aggataaaca gcacactaac aaaggctgtg
     3481 ggcagtggtt tgatgaaaaa cagagtcgaa aacagcaggc tgttgcgcat gctgacacca
     3541 aacagcagca cggttagcag ccaggcgccc aaaaatgctc ctatgaaacc gaactgtgga
     3601 agttccatcc cctgcgcggc tgccgcggct gccagcacca aagcagcggt gccaagatac
     3661 gcaatactgg gggccagacg cttcaggatc attgtgaaaa ccttgtcaga gctctttttt
     3721 gatctcctgc agccgctctt tgttttggtc aaagacgttt ttgatcaacg tgaggaccac
     3781 cgctgccacc aaagccagcc cgaaagcaat caggcagagc attgcccgct tgggacgaac
     3841 tcgcatccca gcttctcgcg gagtgtcaag aatgtccaat gtgggcatgt cacgcagctc
     3901 ttccaaccgg gctgcctcga actgaggata gaggaattca aagactttta actggatttc
     3961 caggttcatt ttcagttggg caaattgggt gccgaggttt ggcaggctgt ctatatcgat
     4021 caaatatcgc ggcttaagcg ctccgcttcc ggcttccaga tccctgatct gccggcccaa
     4081 agcatcgctg cgggatttca attcagctac aattggtgag tccggcgcat agtttttcct
     4141 cgccagttcc agctcgatgt ccgtctgcat cttggctgcg atcacatcgg aatatgatga
     4201 gatcagcgat gccgtctggg tttcgatgtc gatggcgtta tgccgggtct ggaaatcctt
     4261 caccgcgtaa agcagggaat cgatttccgc ttctgtttcg cgcacccggc cttccagaaa
     4321 ctcacggttc atcttgccct tggtgagttt ctgctccttg ttgtagattt ccaatttctg
     4381 cagatagtag tccaccatgt cgcgcgaaag atttttcctc ttggtttccg cgcttacgct
     4441 caccagcccc gtatcggtgc tgtagtcgat gctcatggtc tttttgcgca gttttttcag
     4501 ggccaagtcc atcgccttca gcgaatcctt ttcggttatc ttgtaatact cgataaggtg
     4561 gaagcggcgg atcacatctt cggaaaaacc tctggagcgc atcgcagaaa cggagttgat
     4621 ggcattttgc gcgttgtcgg aacccaaaag cttggaggtg agcccactca aaccttcaat
     4681 gttgaaaggc aacgcgctgg cctggtctcc caccacataa aaactggcgc tggattccca
     4741 gatttgcggc gtcagcaggc tgtaaatcac agccccaatg ctcaccaccg ccacaaagat
     4801 gatgatgaac ctgcggtttt ttatcactat tctcaacagt tcaaagaagt cgaactcttt
     4861 tttgtccatg gcttccatcc tagttcaatc caacccttat ctcaactggc gccaccgaac
     4921 tgggccagat atcccgtcag gggagttggc aagtccaccc ctcccgtggc attttgatag
     4981 ttttccagca gcgcgatgaa cagccgcggc gttgccagcc ccgatccgtt caaagtatgc
     5041 aggaagcgcg gttttccgtc tttgtcccgg tagcggatat tggcgcgacg ggcctgaaaa
     5101 tcatcaaaat tgctcactga ggaaacttcc agatatttgc ccactcccgg cgcccagacc
     5161 tcgaggtcat aggttttcgc ggaggcgaag ctcaggtcgc cggtacagag ctgtaccaca
     5221 cggtaatgca aacccagcgc ctgaaggatg cgctcggcat ccagaagcat ctcttccaaa
     5281 gcattatagg aactctccgg ctccaccagg cgcaccattt ccactttgtt gaactggtgc
     5341 agcctctgca ggccgcgtgt gtccttgccg taggagcctg cttcgcgccg gaaacagggc
     5401 gtgtaagaca catatttcag aggaagttgg tcctgccgga gcacctcgtc cgcgtgaagg
     5461 tttgtcaccg gcacttccgc tgtgggaatc agaaacaggt catccaggtc cacccggtac
     5521 atatcatctt ccaattttgg aagttgtccg gttccagtca tggtcttgcg gtttaccaaa
     5581 accgggacgg acacctcagt gtagccatgt tcctggatgt gaatgttaag catgaaattg
     5641 atcaaggccc gttccagtgc ggcgcccctg ccggtgtaaa ccggaaagcc ggacccgctg
     5701 agcttggcgc cccggggaag gtctaaaagc cggttccgtt cagcgatttc caggtggtca
     5761 aggggctcaa agtcgaaaca gggtttgctg ccccattccc tgactatctt atttgcggat
     5821 tcgtcggaac ccaccggcac agagggatga ggaacattgg ggacttgcaa cagcaggttt
     5881 tccagccggg cgttggcttc gtttagggcg gtgttcaggg tcttgatttc atccgccacc
     5941 gcgctcatct cctgtagtaa atctgttgcg tcttctttgg ccttcttctt ttgggcgatg
     6001 gtctgggaaa cctgattttg cctggttttg agctggtcaa aatcgaactg cagtttgcgc
     6061 cgtagctcat ctgatttcag cagggcgtcc aggtccgcct tttcattttt attgaggatc
     6121 gcgttccgga ccagttccgg attggcgcga ataaacttta tatccaacat gttcagtatc
     6181 cttggcttac ctcaacggcg atgccgatca tggcttgaaa ggattcaact tgtaaagacg
     6241 cgccgccgat caggcctccg tcgatgtctt cctgtcgcag cagtccccgg aggttttcgg
     6301 gtttcacgct gccgccgtag aggatgtgga tattttgcgc ggtctctagc ccatagcggt
     6361 tttgcagcca ggcgcggatc agggcgtgaa cttcctgggc ttgctcggcg ctggcggttt
     6421 ttcctgtgcc gatcgcccac acaggctcat aggcgatcac ggtttccttg cccgcgggca
     6481 tgtcgatttg ctcaaagcag ccttccaact gcttcaggat cacctgctcc gtttcgcctt
     6541 gctcgcgctg ctccagggtt tcgccgatgc acacgatcgg ggcgatcccg ttttcccgta
     6601 gtcgaagcag cttttcgcgg atcagggcat ccgtttcagc atggtactgg cgcctttcgg
     6661 aatggccgat gatgcagtga gttatgccca gtgaactcag ttgtgtcgct gaaacctcac
     6721 cggtgtaagc gccttcattg tgactgctga catcctgggc ggcaatggac gcaggaaggt
     6781 cccgcaggat attccgggcc tgcgccagaa aagtatggac cggcgcgacc aaaggcaaaa
     6841 cccgctccgc gtcatgctcc ttcaggtatg aggctaattc ccggcagaaa tgctccgttt
     6901 cggaagcgtc cttgttcatc ttccagttgc ccgcgatgat gatcttgcgc atgtttaaac
     6961 cacctatctt gttatcattg aaaaatcact tggaaagaaa tgcccggatt cgtcaaggaa
     7021 attgtgtcaa cgcagtgaca atggcacagg aagacgggaa aaaccaagct tgcgaaatgc
     7081 cgctccggaa agcaaggctg gagtagacat caagaaacct tacgtattct atctgtcttt
     7141 gtatcaacct gcctggcaag atgttagcaa atgtgaaacc cccaccaaaa ccgctgccac
     7201 gatcaggaac aattgaaaga aagtgatgcc gcggcgtttg tgtctggagg caactattac
     7261 ttgatcacgc aaacggatga tcagtccgct gatcagcagg gaaaatagca ggatcagcac
     7321 aatctcggac cagcggggga tacggcttat gctcctggtc acgttgaaat tatcgaccaa
     7381 gctgccggtc atttctgaca ggacaggctg cagacgctgg atagtggtct ccttgccgcg
     7441 cagcgtgaaa gtgaagctac ggccctctcc gttcaccgtt agggaataat agctccaaac
     7501 tcccggctgg ccgggattgg tgacgctcgc cgtgaggacc agcattcttt tcagcccctg
     7561 cctcacatag ctggtttcca gcgtcttgac cagcgcgcca ggcggcaaca gcatggtttg
     7621 gaaccttttc tccagaaggt tcatggcgtc gtcatcgaat ctgtctattt tagggccaaa
     7681 gttcaccata accagcccag gaccttcttc gccgctgctt tccatgaacg cggccttgat
     7741 caccaggctg tcgatctggt tgaggacaaa gtttgtgtgc gagttggcca gcgaaaggct
     7801 gtcagcaccc gcgggcagca gttcggaacg gaccgcctcg gtattgctcc ggtccaatct
     7861 gagccaggcc tgcggatatt catgctcgaa attcagcgtg cttctgtaac gcaccatgct
     7921 tggcaacggt gattccgcca ccaaaaaacc catgctgccc gtcagcagta caatcaaaat
     7981 ggccaagcgg attgttagac tgccaaaccc catctattgt ttgcctctaa aaatcattat
     8041 agtaacctcg taaaaactaa caatgtgttg tcggacattg ttttttactc gccaatagtt
     8101 gtcaacctaa atttcgcttt ggctatatgc tgtcctgtgg gtttttacct gcatgccttc
     8161 tactcgcggt tgaggtcaga acagacctct gatatcccca tcttcttcca cgtcaatggt
     8221 ttcggcagcc ggatgtttcg gcagtcccgg catgcgcatg atttcacctg tgatcggcac
     8281 cagaaagccg gcgccactgg aaatcactat cctgcggacg gtcaccaaaa aatccttggg
     8341 gcgtccgatc agttcagggt tgtcggaaag cgatttctgg gttttggcga tacagattgg
     8401 cagcttgtcg taaccgtatt tgttgattct tttcaaatcg gcttttgctt ccatggtata
     8461 gtcaacattc tgtgcgccat agatctggct ggcgatggtg aatatcttat ccttcactgg
     8521 gctgtcccag tcgtaaaggc cgtgatagtg gcatatctca ttgtccacca tgccgatcac
     8581 ctgctctgcc agctccaagc cgcctttccc gccctctgcg tggtaattca caacggatga
     8641 ctcaaagcct ttggaggcaa caaaatcgct caccagtccg atttcctcgt ctgtatcgtt
     8701 tggaaagcgg ttgatggcca cgattgactt catgccgaat ttgtcgatgt tctccagatg
     8761 cttggctagg ttttccagtc cggcttccac ggctttggga tcaggttcgt tgatatcctt
     8821 taatctcttg ccgccgtgaa gcttgagagc ccgaatcgtg gctaccagca ccacggcatc
     8881 cgtgcagagt ttgccatagg ggcacaccag atcgaaaaac ttctccgcgc ccaggtcgaa
     8941 gccgaaaccc gcttccgtta taacgtactc cgaaaggcgc agcgcggctt tggtggcgat
     9001 gatggaattg gcgccctggg cgatgttggc aaatggacct ccgtgaacga aggcaggggt
     9061 gttttctgtg gtctgcacca gatttggctg caacgcgtct ttcagcaggg cggcgatcgc
     9121 gccctcgtaa ccaagctgat tcacgtgcac cggctcaccg tcaaaggtga agccgacagt
     9181 gatgttgccg atcttttcct tcaattcctc catgttgttt gacaggcaaa gaatggccat
     9241 gatttcgctg gcgggggtga tgtcaaaacc gtcttcccgc ggaaaacctt gtttgctgcc
     9301 tcccagtccg atcacgattt tgcgcagcat gcggtcattc acgtccagca ctcttttcca
     9361 ggtgatgcgc cggggatcga ggttgagctc gttgttaaaa tagatatagt tgtccaccag
     9421 cgccgcgagg gtgttgtttg cggtggtgat ggcgtgaaaa tcgcccgtga aatgcaggtt
     9481 gatgtcctcc atcggcaaaa cctggctcca accgccgcca gccgcgcctc ccttgatgcc
     9541 gaaaaccggt cccagagaag gttcacggat ggccactatc gagcttttgc cgctcaggtt
     9601 c
//
LOCUS       DAOOWR010000017         9412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7254_length_9412_cov_3.916747, whole genome shotgun sequence.
ACCESSION   DAOOWR010000017 DAOOWR010000000
VERSION     DAOOWR010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7254_length_9412_cov_3.916747"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /gene="der"
                     /locus_tag="PLB85_00980"
     CDS             complement(<1..1122)
                     /gene="der"
                     /locus_tag="PLB85_00980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU71929.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRSAIVDPEAGITRDRKYE
                     DVEWNGKVFKIVDTGGIVFDSGEAMDKMIRHQAQLAIDESDLIIFMVDSQTGTTDIDK
                     AIAKILFPHRDKVMLVANKADNEKFEWELFDFLQLGFGDPFPISASQARNIGNFKDEL
                     ISLIPETQNAYEDDTPAHTRIAVVGKPNVGKSSIVNLLLGNEKQIVTEIPGTTRDAVD
                     SFFRYHGQEYVLIDTAGLRRKSKVNYGVEYYSVMRTIEAVDRCDVVVLVLTADEPISD
                     QDVKIASYAKRRMKEILVVFNKWDLVEKDSKTTGKFIADLHEMMPFLQFAPVQFISAK
                     TGQRINRIMETVVKIEEESEKRISTSELNRFMETVVQHRP"
     gene            1533..2318
                     /locus_tag="PLB85_00985"
     CDS             1533..2318
                     /locus_tag="PLB85_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU71930.1"
                     /translation="MNGRCALVLLALLLCVSGLDAGEEGGHFRRYLASYPLSVKEAAT
                     APARWQAKEWVTAGGVALVAGSLIWADLQIRGFAQDNRTSQGDAFLDIFAYTGDKWVL
                     FPAAGATALAGWATGSDKTTDTGLLCLKSMLMTAVATEGLKLATQRQSPGAESDGSFW
                     PEGGFSLENGSFPSGHSAMAWSVAPVLAEQYSDQTWVTPLVYSVAVLSSLSRVNADEH
                     WASDVFCGAVIGYLAARLTLNSTPRLALKPAPELNGISLGLSF"
     gene            complement(2529..3152)
                     /locus_tag="PLB85_00990"
     CDS             complement(2529..3152)
                     /locus_tag="PLB85_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOU71931.1"
                     /translation="MNKRVLIFLAAVLAVASLMGQVVASEASKPQMKTRMEALEPFIV
                     MGLEAQNAMEGEAMMEAWTKFFSYHEKLPEPVDNNYYGIYYPGEKFDLSTMQGYNYLV
                     GMEVKDEVKLPEGLQLHKVPGGNYAVFEYVGPIHEIGNAYEYIYGEWLATTNYKPASM
                     EMFEVYGERFKEDSKDSVVEIWVPVQKPSLEEAPQDGDIQEGKKLEK"
     gene            complement(3340..4347)
                     /locus_tag="PLB85_00995"
     CDS             complement(3340..4347)
                     /locus_tag="PLB85_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU71932.1"
                     /translation="MKNISKILVIALALMAFSAAMFALEQTVGLRFGCSHPKNDISVD
                     YPDHGIVNFMAGLNYEAWLKDYISMGIYPYYTKLENDSNINGYAAKVIGAEIQARFRP
                     TKVAVVNFKDGALQRIAPYAQIGIGAAYVDNDNMLDGQFAFLAPTAGLGLSFQTKWNI
                     DFDLGVQLDHALTDEIDNQVGGSDIFTDAHMMPYLGIGYTFGKKGGSSDAVVSRLLRN
                     VVSMEQDFTLDGVQFEFNSSKLTGDAKVVLQEVIEAMKKNPNVRLEIHGHSDNVGDSA
                     YNITLSQERAQSVKDYMVQNGISANRLTTKGFGMNKPIASNSTPEGRALNRRIEFVIV
                     K"
     gene            4689..5555
                     /locus_tag="PLB85_01000"
     CDS             4689..5555
                     /locus_tag="PLB85_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU71933.1"
                     /translation="MKLFETHAHLDLPDFNADRESLINKCFESGIEYIINIGFNKETS
                     LNSLELAKKHLHIFATVGFHPHDAMDFDAELIKRLVRDKNVLAIGEVGLDFFRNYSPY
                     PVQREVFRNQAYLAVDYDKPLVIHNRDAHQECYNTLKEVQAKDVVFHCFSGDIIFAQQ
                     VFDEGWMVSFTGNVTYPNSNLEDVVRMMPMDQFMIETDCPYLTPHPHRGKRNSPLHLH
                     LVAEKIAEIRGITPLEVAQASFDNAFRFFRVPPDPVRPISKKGGSKGGKSSKKAKTGK
                     TTTKAKDKKKDK"
     gene            5560..6786
                     /locus_tag="PLB85_01005"
     CDS             5560..6786
                     /locus_tag="PLB85_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71934.1"
                     /translation="MKTKLLLPLLLLLSLPLLAGNSAFAFDGNALRYYGNDIYSLGMG
                     DTGASDVFRLNSGYGNPAMHNLNNRSLFSTGLMPGYNHYNSQDSLGNKFSHLDNSLDF
                     PYFSLSIPIKQHRLGFQFSSHSSGVVDNQREFTLIQEPDTLSVTEKHSMDRYIYRADL
                     IYSFAWGRNSLGISGNYYLGHEIRNFKQTAGFGAFDTEEEIVRDYKNPGFTLGYIRHQ
                     EQFAVGAYYSLGVNMKGEEVRTSIHSTEDPLPYEFRIPPVYSASVTALPYPEFKLAAD
                     FHYEPWQKIDEAKYTDSWKAALGLAFEPAAREERKGLLSLPLRTGVSWRHLPFRAAGS
                     EINELALSLGVSLPLKGEVNRIDVGFQYSHRGKLDQNKLLDNSYLLMFGFTGFDILSK
                     APNRTAPREIPEKEDL"
     gene            6870..8348
                     /locus_tag="PLB85_01010"
     CDS             6870..8348
                     /locus_tag="PLB85_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU71935.1"
                     /translation="MSVGLETLPDEALLANINSVAQRISRAMFANEPTVIFGHDDPDG
                     ITSTYVMYQFFNSCGYQRHNYFIPNRNLEPHGIQEGFVRFVREGGYKLVITVDNGISS
                     YGGVEKLNALDCEVVITDHHLVQPETLPNAYAILNPQLPGCQYPFKPLAGVGVALMLA
                     RWLGRSLEHQVPLSSYFWTAVGSIADKVPMTGVNRTIVRHVINHWDDMNDPSVDFLMR
                     NHKRIENDMDVFNFMQYTSRLIANGRESGGQHTAMRFLLQMGDAKAELFQAMEAKQKK
                     WEGELNRVFNILDSVTAGFSSNAFIYFDDEGVIPYPLLGTGATYVLGKLGIPAILLKQ
                     HNGDIVCEGRCGEGFNMVEAFASCKEHLKQFGGHAKAAGFTLEPHNYDAFLECYNHYL
                     AGNLSHNTEPIQEEPDACLTLDQFTSDNWRGLELLLPFGQQHPEPRILVENVRLEELQ
                     KLFMLEHGSVSLPLGQAGNTLLHWRSPKQVRVLSFEPADSTA"
     gene            8368..8973
                     /locus_tag="PLB85_01015"
     CDS             8368..8973
                     /locus_tag="PLB85_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOU71936.1"
                     /translation="MLKVKETLRKSIHLSSLVIPYGYRFILKDNRKLMFYVMLSAFAI
                     MLVVEFYRFWQKSFRKTFWWIFGLVLRRHELRDFTGATYLLFSAMLCVAFFEPVIVFF
                     ALSFLALGDTFAAMVGINFGRRKIGKGNKSLEGSLACFAVCFSYALLFGLHPDFYLHP
                     VVAFTGALAATLAEMSGLPVDDTVEMPLVSALAMALAKIIF"
     gene            8985..>9412
                     /locus_tag="PLB85_01020"
     CDS             8985..>9412
                     /locus_tag="PLB85_01020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU71937.1"
                     /translation="MLLSFVIPVLNEEDSLLRLTAEIVANCKPHAHEIIFIDDGSRDK
                     SFSIMEELAKADPQIRIVKFRRNFGKAAALQYGFRLARGEVVFTLDADLQDNPVEIPR
                     FLAKLDEGWDLVSGWKQKRHDPLHKVVPSRFFNFVTARAFR"
BASE COUNT         2081 a         2472 c         2521 g         2338 t
ORIGIN      
        1 tgggcggtgt tgcacaacgg tttccataaa gcggttaagt tcgctggtgg agatgcgttt
       61 ttcgctttcc tcttcgattt ttaccaccgt ttccatgatc cggttgatgc gctgtccggt
      121 cttggcggaa atgaactgca ccggtgcgaa ttgcagaaag ggcatcattt catgcaggtc
      181 ggcgatgaat ttgcctgtgg tcttgctgtc tttttccacc aggtcccact tgttgaagac
      241 caccaggatt tccttcatgc ggcgtttggc ataggaggcg attttcacgt cctggtcaga
      301 gattggctcg tctgcggtga gcaccagcac caccacgtcg catctgtcca cggcttcgat
      361 ggtgcgcatc acgctgtagt attccacacc gtaattcact ttggatttgc ggcgcaatcc
      421 tgcggtgtcg ataagcacgt attcctggcc gtgatagcgg aaaaagctgt ccacggcgtc
      481 gcgggtggtg ccgggaattt cggttacgat ctgtttttca ttgcccaaaa gcaggttcac
      541 tatggaggat ttgcccacgt tcggttttcc caccacggcg atgcgggtgt gggcaggtgt
      601 gtcatcttca taggcattct gcgtttccgg gatcagggaa atcagttcat ccttgaaatt
      661 accgatgtta cgggcctggg aagcggaaat ggggaagggg tcgccaaacc cgagctgaag
      721 aaaatcaaaa agctcccatt cgaatttctc gttgtcggcc ttgttcgcga ccagcatcac
      781 cttatccctg tgcgggaaga ggattttggc gatggccttg tcgatgtccg tggtgccggt
      841 ctgggagtca accatgaaga tgatgaggtc ggattcgtcg atcgccagtt gcgcctggtg
      901 gcggatcatt ttgtccatgg cttcgccgct gtcaaataca atcccgcctg tgtccacgat
      961 tttgaacact ttgccgttcc actccacatc ctcgtatttg cggtcgcggg tgatccccgc
     1021 ttccgggtca acgatggcgg aacgctttct gcaaaggcgg ttgaacagcg tggacttgcc
     1081 cacattagga cgacccacga tcgatacgat gtatttcctc attcagaagt ttacttcccg
     1141 gttgcttttg gcgccactct tttcaaaggg ggtattttgt caatcccata atgggttacg
     1201 cggcctcgcg tgaagccggt cttttcgggc ttggaacgac ctgcgttttc acagagttgt
     1261 tacccactat ttttaagaga tgggtccagc atttgcggga ttttcgctgg ctttttgccg
     1321 gactcgtcct ttgccctttc ccatttgcat cctgatcgca tctcgctgag ttcccgcctg
     1381 gcaagcggga agtcaacggg aagtgaacgg gatgtgagtg ggatggggca aagggtgtaa
     1441 ccaggctgtt acgcaggatg ttacagccag taattgtctc cgagtcagga taaatccttg
     1501 acggaaatgg tgacgtggaa attggtcgcg gcatgaatgg aagatgtgcc cttgttttgc
     1561 tcgctttgct gctctgcgtt tccggtttgg acgccgggga ggagggcggc catttccggc
     1621 gttatctggc ttcctatcct ctcagcgtga aggaagcagc aacagcgccc gcgcgctggc
     1681 aggccaaaga atgggttacg gccggcggag tggcgttggt ggcaggaagt ctgatctggg
     1741 ctgatctcca aatccgcggt ttcgcccagg acaacagaac cagccagggt gacgccttcc
     1801 ttgacatttt tgcctacacc ggggacaagt gggtgctttt ccccgcggcg ggggcaaccg
     1861 ccttggctgg ttgggcgacc ggttcggata aaacaaccga caccggtttg ctgtgcctga
     1921 aaagcatgtt gatgaccgct gtggcgaccg agggcctgaa actggccacc cagaggcaaa
     1981 gtcccggggc ggaaagcgat ggaagcttct ggccggaagg cggcttttcc ctggaaaacg
     2041 gttccttccc ctccggccac agcgcaatgg cctggagcgt cgcgccggtt ctggcggagc
     2101 agtattcaga ccagacctgg gtcaccccgc tggtttacag tgtggctgtt ctgagttcct
     2161 tgtcccgggt taacgctgat gaacattggg ccagcgacgt tttttgcggt gcagtgatcg
     2221 gctaccttgc cgcccgactg acgctgaaca gcactccccg cctggcgttg aagccagcgc
     2281 ctgagctgaa tgggatcagc ctcggcttga gtttttgaag gctgggagag ccggtcagaa
     2341 cttcagcggt cgggctgctg gtcttggatc gctatctccc ggtccagata gatgtcccga
     2401 ttcagcatcc tgtgacttgg gctgattttc atcagttatt gttgtgctaa cttcagatac
     2461 aacaatccct tcgcgccaaa caacgaatcc gcgctatcca gaaatgtcct tagctatcag
     2521 gcagggcttt acttttccag tttcttccct tcctggatgt ccccatcctg cggagcttct
     2581 tccagactgg gtttctgcac cggcacccaa atttcaacta ccgaatcctt tgaatcttcc
     2641 ttgaagcgtt cgccatagac ttcaaacatc tccatggaag caggtttgta attggtggtg
     2701 gccagccatt cgccgtagat gtattcgtaa gcattgccga tttcatggat cggcccgacg
     2761 tattcaaaca cggcgtagtt accgcccggg accttgtgta gttgcaggcc ttcgggcagt
     2821 ttcacctcgt ctttcacttc catacctacc aagtaattgt agccctgcat ggtggatagg
     2881 tcgaatttct cgcccggata gtaaataccg tagtaattat tgtccactgg ctccggcagt
     2941 ttttcgtgat aagagaagaa cttggtccag gcttccatca tggcttcgcc ttccatggca
     3001 ttttgcgcct ccaaccccat cacaatgaag ggctcgaggg cttccatacg ggttttcatc
     3061 tggggtttac ttgcttcgga ggcaacaact tgccccatca gagaagctac ggccaagaca
     3121 gccgccagaa aaatcaagac acgcttattc atgatatcct ccactattat tgaagtagtg
     3181 gattgcaaat taacagcgaa tcaattagac gtcaaggatt ttttgtaagg tgagcgcgaa
     3241 aagctctggg ttggccttcg taaaggatga tcctggcacg gtgcaaaaaa gaggccccga
     3301 gaaaatcagg ggcctctaat aagctctggt ttaagcggat tacttgacga tcacgaactc
     3361 aatgcggcgg ttgagggcac ggccttcagg agtggagttg ctggcgatcg gtttgttcat
     3421 gccaaaacct ttggtggtga ggcggttggc agaaatgccg ttttggacca tgtagtcttt
     3481 cacagactgg gcgcgttcct gggacagggt gatgttgtag gcggaatcgc caacgttgtc
     3541 agagtggccg tgaatctcaa gtctcacgtt cggatttttt ttcatggctt caataacttc
     3601 ctgcaatacc actttggcgt cgccggtaag cttggagctg ttaaactcga attgcacgcc
     3661 gtcgagggtg aagtcctgct ccatgctcac aacgttgcgc agaaggcggg aaacgacggc
     3721 gtcgctgctg ccgccttttt tgccgaaggt gtagccgata cccagatagg gcatcatatg
     3781 agcgtcagta aaaatgtcgc tgccgcccac ttggttgtca atttcgtctg tgagggcatg
     3841 gtcaagctgc actcccagat caaaatcgat attccatttg gtttggaagg aaaggccgag
     3901 tccggctgtg ggagcgagga acgcgaattg gccatccagc atattgtcgt tgtcaacata
     3961 agccgcgccg atgccgatct gggcataggg ggctatgcgc tgcaaagctc cgtctttgaa
     4021 gttcacaacg gcaactttgg tggggcggaa acgggcctgg atctcagcgc cgatgacttt
     4081 ggctgcgtat ccattaatgt tgctatcatt ctcaagcttg gtataatagg gataaatacc
     4141 catgctaata taatctttca gccaagcttc ataattcagg ccagccatga agttgacaat
     4201 tccgtgatcc ggataatcaa cgctgatgtc atttttggga tggctgcagc cgaaacgcag
     4261 gccaacagtt tgttcaaggg cgaacatagc cgcggaaaag gccattaatg caagggctat
     4321 aaccagaatt ttactgatgt ttttcatttt tgtacctcgt ggtattgtaa attctgaatc
     4381 ttattcccca aagcaccctt ttctgtcaat ctaatttttc ctttcctaat tttggacatt
     4441 gctttttaag gtgtcagctt aggttgctat gaccttgttt acggctgcgc tttcaaccgc
     4501 tgtaccaggt ttgggacggt cagcggtctt atcgaggcag tttggagtca atagataatc
     4561 tgctgtgttt tccggaaggg acaaaaagcc gcgggtgtca aacgggggcg agagttcttt
     4621 tcaattgaca agaaagcggc atcttgtttt tcttgtttta aatctaaatc agggataaaa
     4681 tgctgtaaat gaaactgttt gaaacgcatg cccatctcga ccttccggat ttcaatgccg
     4741 accgggagag cctgatcaat aaatgctttg agagcgggat cgagtatatc atcaacatcg
     4801 gtttcaacaa ggagacctcg ctcaactcgc tggaactggc caaaaagcat ttgcacatct
     4861 ttgccacggt tggctttcac ccgcacgatg ccatggattt tgacgccgag cttatcaagc
     4921 gtctggtgcg ggacaaaaat gtgctggcca tcggtgaggt tgggctggat tttttccgca
     4981 actattcgcc ttacccggtg cagcgtgaag ttttccgcaa tcaagcatat ctggctgtgg
     5041 attatgacaa acccctggtg atccacaacc gcgacgcgca ccaggaatgc tacaataccc
     5101 tcaaggaagt gcaagccaaa gatgtggtct ttcactgctt ttcgggcgac attatctttg
     5161 cccagcaggt gtttgacgaa ggctggatgg tctccttcac cggcaatgtg acctacccaa
     5221 actccaatct ggaggatgtg gtgcgcatga tgccgatgga ccagttcatg atcgagacag
     5281 attgtcccta cctgaccccg catccgcatc gtggcaagcg taacagccct ctgcatctgc
     5341 atctggtggc ggaaaagatc gctgaaatca ggggcatcac cccgttggaa gtagcccaag
     5401 cctcgttcga taacgccttc cgctttttta gggtcccgcc agatcccgtg cgccctattt
     5461 ccaagaaggg aggttccaaa ggcggaaaat cctccaagaa ggccaaaacg ggcaaaacca
     5521 ctaccaaagc caaagacaag aaaaaggaca agtgatattt tgaagaccaa acttcttctt
     5581 cccctgttgc tactgctcag tctgccactg ctggccggga actccgcctt cgctttcgac
     5641 ggcaatgccc tgcgctatta cggaaacgac atctacagcc tgggcatggg ggacaccggc
     5701 gccagtgatg tgtttcgcct caactctggc tacggaaacc ccgccatgca caacctgaac
     5761 aaccgcagcc tcttctccac cgggctgatg ccgggctaca atcattacaa ctcccaagac
     5821 tctctgggca acaagttcag ccatctggac aattcgctgg actttcccta tttcagcctg
     5881 agtatcccga taaagcagca ccggcttggt ttccaattca gttcccactc ctcaggcgtg
     5941 gtggacaacc agcgcgagtt caccctcatt caggagccgg acacactttc ggttacggaa
     6001 aagcactcca tggaccgcta tatctaccga gcggacctga tctacagctt tgcctggggg
     6061 cgaaacagcc tggggataag cggcaactat tatctgggcc atgagatacg caattttaag
     6121 cagaccgcgg gattcggcgc atttgacacc gaagaggaga ttgtgcggga ctacaaaaac
     6181 cccggcttca cgcttggcta tatcaggcat caggaacaat tcgccgtggg tgcctattac
     6241 agcttgggag tgaacatgaa gggcgaggag gtgcgcactt ccatccactc aacggaagac
     6301 cctttgccct acgaattcag gattccgcca gtttattcgg ccagcgtgac tgctttgcca
     6361 tatccagagt tcaagttggc ggctgatttt cactatgaac cctggcagaa gatcgacgag
     6421 gccaaataca ccgatagctg gaaagccgct cttggcttgg catttgagcc tgccgcgcgg
     6481 gaagagcgaa aaggcctgct cagcctgcct ctgcgcacag gggtttcctg gcgccatctg
     6541 ccgttccggg cagccggcag cgagatcaat gaactagccc tcagcctggg cgtaagcctg
     6601 cccctcaagg gcgaggtgaa ccgcatcgac gtgggcttcc aatatagcca ccggggtaag
     6661 cttgaccaga acaaactttt ggacaattca tacctgctga tgttcggatt caccggcttc
     6721 gacatccttt ccaaagcccc caaccgcaca gcaccgcggg aaataccgga aaaggaagac
     6781 ctgtaaacat ggtaggagcg catccggact cagatcccaa cagtctttta cgccaaatct
     6841 gtcggaagcg gggcatcgac caggacgggt tgtcagtcgg gcttgaaaca ctgcccgacg
     6901 aagccctgct agccaacatc aattctgtgg cgcagcgcat ctcccgggca atgtttgcca
     6961 atgaacccac cgtgatcttt ggtcacgacg atcctgacgg catcaccagc acctacgtga
     7021 tgtatcagtt tttcaattcc tgcggatatc aaaggcacaa ctatttcatt cccaaccgca
     7081 atctcgaacc ccacggcatc caggaaggtt ttgtacgctt cgtgagagag ggtggctaca
     7141 aactggtgat cactgttgac aacgggattt ccagctatgg gggggtggaa aagctgaacg
     7201 ctcttgattg tgaggtggtg attacggatc accatcttgt ccagccggaa accctgccca
     7261 atgcttatgc catcctcaat ccgcaattgc ctggctgcca atatcctttc aaacccctgg
     7321 ccggggtagg ggtcgccctg atgcttgcca ggtggctggg acgctctctg gagcatcaag
     7381 tgccgctatc ctcctatttt tggacggcgg taggctcgat agcggacaaa gttccgatga
     7441 ccggtgtgaa caggaccata gtccggcatg tgatcaatca ttgggacgat atgaacgatc
     7501 ccagcgtgga ttttctgatg cgtaaccata aacgcataga aaatgacatg gatgtgttca
     7561 attttatgca atacacttcc cggctgatag caaacggccg agagagcgga ggacagcaca
     7621 ccgcaatgcg ttttcttttg cagatgggag acgccaaagc cgagcttttc caggccatgg
     7681 aagccaaaca gaagaagtgg gagggtgaac tgaaccgtgt tttcaacatt ctggacagcg
     7741 tcaccgccgg cttctccagc aacgccttca tctatttcga tgacgaaggc gtgatccctt
     7801 atcccctgct gggaacaggc gccacctacg ttctgggcaa attggggatt ccagccatcc
     7861 tgctcaagca gcacaatggc gacatagttt gcgagggacg ctgcggagag ggattcaaca
     7921 tggtggaggc tttcgcttcc tgcaaggaac acctgaaaca gttcggcggc cacgccaagg
     7981 cggctggttt caccctggaa ccccacaatt acgacgcttt tctggaatgc tacaaccatt
     8041 atctggccgg aaacctgagc cacaatacag agccaattca ggaagaacct gatgcctgcc
     8101 tgacactgga tcagttcaca tccgacaact ggcggggctt ggaattgctg cttcctttcg
     8161 gtcagcagca tccggaacca cgcatccttg tggaaaacgt tcggcttgag gagttgcaga
     8221 agctcttcat gctcgaacac ggcagcgttt cccttcctct tggccaagcc ggaaacacgc
     8281 tactgcactg gagaagcccc aagcaggtga gggtcctttc ttttgaacca gcagattcca
     8341 ccgcgtgagg cagcgcggaa aagaacaatg ctcaaggtaa aggaaaccct gcgcaaaagc
     8401 attcacctgt cttccctggt gataccttac ggttaccggt ttatcctgaa ggacaaccgc
     8461 aagcttatgt tttatgttat gctctcggct ttcgccatta tgctggtagt tgagttttac
     8521 cgcttctggc agaaaagctt ccgcaaaact ttctggtgga tattcggcct ggtgctgcgc
     8581 cgccatgaat tgcgcgactt caccggtgca acctatctgc ttttctcagc catgctttgt
     8641 gtggcctttt tcgagcctgt gatagtgttc tttgccttgt cgttcctggc tttgggtgat
     8701 acttttgccg cgatggtggg catcaatttc ggtagacgca agattggcaa agggaataag
     8761 tcgctggagg ggagcctggc ctgcttcgca gtctgtttca gctacgccct cctctttggt
     8821 ttgcatcctg atttctattt gcatccagtt gtggcgttca ccggggctct ggcagccact
     8881 ttggccgaga tgagcgggct gccggttgat gatactgtgg aaatgccgct cgtgtcggct
     8941 ctggcgatgg ccctcgccaa gatcattttc tgaaaggttt gaacatgttg ctatcttttg
     9001 tgatcccggt tttgaacgaa gaggattccc tgctccggct caccgctgag attgtggcca
     9061 actgcaagcc ccacgcccat gagataattt tcattgacga cggcagcagg gataaaagct
     9121 tctccataat ggaggaattg gccaaagccg atccccaaat ccggattgtg aagtttcgcc
     9181 gcaattttgg caaggcggca gccctgcaat acggtttcag gctggcccgg ggagaggtcg
     9241 tgttcactct ggacgccgat ctgcaggata atcctgtgga gattccgaga ttcctggcca
     9301 agctggatga aggctgggac ctcgtttccg gatggaagca gaaacgccac gaccctctgc
     9361 acaaagtggt cccctcccgt ttcttcaatt tcgtcaccgc ccgcgccttc cg
//
LOCUS       DAOOWR010000018         9404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7263_length_9404_cov_5.889079, whole genome shotgun sequence.
ACCESSION   DAOOWR010000018 DAOOWR010000000
VERSION     DAOOWR010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7263_length_9404_cov_5.889079"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLB85_01025"
     CDS             <1..363
                     /locus_tag="PLB85_01025"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU71938.1"
                     /translation="ADHPGWYVFSPELFARLQSEGKPVFLDIGADWCTNCKSNEKKVL
                     LQPDIQAEFNKRGVVLLKGDFTREDPVLKDWMQKGGSIGVPFNVLYIPGREPVKVAEL
                     FSKADLLKALELIPEKTE"
     gene            360..1463
                     /locus_tag="PLB85_01030"
     CDS             360..1463
                     /locus_tag="PLB85_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71939.1"
                     /translation="MKKFAIPVLVVLAALFVAGCDRFAHNFQPPEEVDLQTGLFDPLN
                     QALQNQPEAGIESVMVHFADDYLHFGLNKSDRRAWLEGIFQAQPNAISEARLLYSNLT
                     TDSTAVSKWRLTIRESEGVIVDSTFVGENLVKRNGHWLLRGNQLISEPPAVKQRVIIE
                     YFTFLGCPNCPAVEAELHNLQLAYPGQLSYLEHHISGPLTVAGDETYDYYGPFTVPTS
                     ILGGGVMLTGSSADIISSYAPEVEALANIDSRIIYSELSYTVDGQTISGTVRLDMPDP
                     FTHTDMVLNCVLIERESSFTNTQGQPLRNVVRAKSVKVIGNLDFPNTVSFSLNSAVPI
                     PDDASLVIFAQHHPAFFGNNAIIYGGIEVELDR"
     gene            1625..2236
                     /locus_tag="PLB85_01035"
     CDS             1625..2236
                     /locus_tag="PLB85_01035"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU71940.1"
                     /translation="MKTKILLLLAILLAGSLLAEPMPEFKLPNESGKSVNLTELLGRG
                     PVIVDFWADYCQPCKQAMPALDALAQKYDSLTVVLISIDAPKAQTKAKNYLKGKNFKF
                     VSLFDPDQTLAKKLNVAEPPHTFILDKAGEIVLEHKGFVAGDEKEYELKVRSLLGFEE
                     EIGHECDCAEPCENCDHKEKTEAAPDQKPAPEKAHKSPCGNCH"
     gene            2218..3684
                     /locus_tag="PLB85_01040"
     CDS             2218..3684
                     /locus_tag="PLB85_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOU71941.1"
                     /translation="MRKLPLIFLAALLLAGTALPAQTSLTLNGLNEASLIYRSVPDSL
                     HVYFQNTFGFSLGYRNFSFGMKFIAELPKYSTNQSELLENLAADRLSLGWKELYASYA
                     KDAYLIHAGIIEESFGSGLIFRSFMDAELDEDHRVTGFKFRYDDKLRLKALYSALPSP
                     TSHGKLDLAYSADAEYPVLEPLTLGASFVGMQTLVGTYYKQDDILGARASLVLDKFEL
                     SAEGGVRDDKLDAGLMDGKAIYTAATAYLGPVQLGGAFKHYDNFAYRNRVQDLPLANH
                     HGEALADDLASGADETGFQGWANIAIGQSLCLALDYAEAWDKAGEKQMNDAYAGLEWQ
                     SGATAATLSYSHVEKVDSALRHWQKESYPAFVVSFPAGKAGLVLSGEFKTVEKLVLET
                     AQNEYVEIGHYEPRLQADLSLGKLSLSLGAQSWWNDFSSILKSHYWPSVELKYPILAN
                     SDLVLFAGKEAGGKVCRNGVCRYVAPFSGVRLELATRF"
     gene            3698..5020
                     /locus_tag="PLB85_01045"
     CDS             3698..5020
                     /locus_tag="PLB85_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU71942.1"
                     /translation="MKKTIFIPLLLLLAALLAADPNYYPLTTIAESCVTENSPDCVSA
                     LANLANVLSATHRGEFIATRLYDTGNLSSPSVADRVDHYGIIQWPTVIMNGTHTLIGV
                     HPETVYAQAVAAKCFQPSPLKLQITSFTPASGAVSVSARLLDHDVNITGQTLMLMLVE
                     DNVGSETNVVRQIKYQTVNIPGSGDPVTFTDSFSIDSTWNSANLWAVAAVHMNDHAIL
                     QSASTLPLPQYNIRAAMPWNPAELVTPANSSLISEPLWVFNTGSSDNMQMQLVADDAP
                     DDWYFNYCDEEGNCYPGSGLMPLELAAGESKAFHLNLWVGSPGVGSFHFEITSANLGT
                     FSIPFVCQAGTEVSDEVLQPSLSLAGNSPNPFRGGTSFTLNAEKSVSASIQIFDAKGR
                     LVAETPAQNLVPGSNRIEWQAPSELPSGIYLYRLKESSAPPRRMLLIK"
     gene            5024..6499
                     /locus_tag="PLB85_01050"
     CDS             5024..6499
                     /locus_tag="PLB85_01050"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU71943.1"
                     /translation="MRRYLVLLLLLAAVALAATPLGKEFEEALRPTQNADQAMEVITT
                     YRDRLTDLEDLRQLQNYWMQIDPDGCRDWFGQQYRAHPESPEFEYLWLRGDADPGTQL
                     LGGRALIAREPSFYWGYRLFSNTYSQLLQAAPDSIRADIIANLAADRDILLQGLKRWP
                     QDDYLHLALFHHYASQGENEQAEAQLLNLHEPAAIEANFRHVIEFIGSSGSVRPFEVL
                     YPLLISRLIAKGEISAADSLDYYQLSYLEALKTARDWPKMCSFLELNPGLQTDDRTLE
                     TRLLMYLGLEQPETALNLLEGALAAGVITYPEALDNPDYAPLAELPRYPEVMALASAN
                     WEKAKAERKAKIISEKTSRPAPLWELPDPDGKLNRLEDLRGKIVILDFWALWCSPCLK
                     TLPKLDSWLERNPSDDVVLISINVWESPSEIPNVIEYFSKNSYGMKLLLGDNELPRAY
                     GFSGIPWLCAIDKQGNIAFTQSGFSPILDEIISVWVEELRR"
     gene            6509..7246
                     /locus_tag="PLB85_01055"
     CDS             6509..7246
                     /locus_tag="PLB85_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOU71944.1"
                     /translation="MMRRPNPYLTPKEQRALLVLAALALLGLAAGKLKTVPAFAPFLQ
                     TEPASPELLSVAAEVDKPVQIDIRTASLEELMLLPGIGPKRAQDIIDYRSQHPFTSTD
                     DLLQIKGIGAKTLAKMLPSLLLFGSPQFTPEAPSDPNSMTTIGLPDPGQNNLAESDAP
                     ASSSSEKSSSRKAKPTPKSQLTNIVNLNTAGLAELCTLPGIGEVKAQAIIDYRSQNGK
                     FQSVDDITKVKGIGAKTLAKLRHRLKV"
     gene            complement(8466..9221)
                     /locus_tag="PLB85_01060"
     CDS             complement(8466..9221)
                     /locus_tag="PLB85_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOU71945.1"
                     /translation="MNKPTIGQRLSLVIKAMHLKDYQFAKKYGISRASLSRYKLNERY
                     PDPEFLEALSKDQINLHWLFTGNGSMYAKDEFQELIQSNQATTSPTNPDVSNPTIISP
                     ILHPITDKNFDPTHSITFPIVGEIAAGPPMEIKDEWSQMGQIQLPVSFLPGNPKQYTV
                     FQVNGRSMEPVLQHQDIVVIKSNLSWENADGKIAVVRTDDGLTIKKVQIDHHRQQIIL
                     QPFNIDYPVLVLDSDWNYGAFLIGTIALQLRFF"
BASE COUNT         2144 a         2923 c         2341 g         1996 t
ORIGIN      
        1 gccgaccatc ccggctggta tgtcttctcg ccggaactct ttgcccgcct tcagtctgaa
       61 ggcaagcccg tcttcctgga catcggggcg gactggtgca cgaactgcaa gtccaacgag
      121 aagaaagtgc tgctccagcc ggatatccag gctgaattca acaaacgcgg ggtggtgctg
      181 ctcaagggag atttcacccg cgaggacccg gttctgaaag actggatgca aaaaggcggc
      241 agcatcggcg tgcccttcaa cgtgctctac atccccggcc gcgagcccgt caaggtggct
      301 gagctcttca gtaaagccga cttgctcaaa gccctggaac tgattccgga gaaaaccgaa
      361 tgaaaaaatt tgccatacct gtcctggtcg ttctggctgc cctgttcgtg gctggttgcg
      421 accgctttgc ccacaatttc cagcccccgg aagaggttga cctgcaaacc gggctgttcg
      481 atcccctgaa ccaggctttg caaaaccagc cggaagccgg gatcgaatct gtaatggtcc
      541 attttgccga tgactatctg catttcgggc tgaataaatc cgaccgccgc gcctggctgg
      601 aaggcatttt ccaggctcag ccgaatgcca tatccgaagc cagattgctg tattccaacc
      661 taaccaccga ttccactgcc gtgtcaaaat ggaggctgac catccgcgaa agcgagggcg
      721 ttatcgttga tagcaccttt gtcggtgaaa acctggttaa acggaacgga cactggctgt
      781 tgcggggaaa tcagttgatt agtgagcctc ctgcggttaa acagcgtgtc atcatcgaat
      841 acttcacctt cctgggctgc cccaactgcc ccgcggtgga ggccgaactc cacaacctgc
      901 aactggctta tcccggccag ctcagctatc tggaacacca tatcagcggc cccctaaccg
      961 tagccggaga tgaaacttat gactattacg gccccttcac cgtgcccacc tccatactgg
     1021 gcggtggggt gatgctcacc ggaagctctg ccgacatcat ttcctcctat gccccggagg
     1081 tggaggccct ggccaatatc gacagccgca tcatctattc ggaattgagc tacacagttg
     1141 acggccagac catcagcggc acggtgcggc tggatatgcc ggaccctttc acccatactg
     1201 acatggtgtt gaattgcgtg ctcatcgagc gcgaatccag cttcaccaac acccagggcc
     1261 agcccctgcg caacgtggtg cgggccaagt ccgtcaaggt cattggcaat ctggacttcc
     1321 ccaacaccgt aagcttcagc ctcaactccg ccgttcccat tccggacgat gcctcgctgg
     1381 tcatcttcgc ccagcatcat ccggctttct tcggcaacaa tgccatcatc tacggcggga
     1441 ttgaggtcga gcttgacaga taaccttatg gataccacct tgacgcctat ggctttgttc
     1501 ctcgcatacc ccccgccaaa tccccgcatt cagtgcgggc atcatgcggg aggcaacccg
     1561 catccgtagc ttaaaggctt caggacggaa cagacaataa tttgaaatac atggagacaa
     1621 aaacatgaaa actaagattc tactattact cgcgattctc ttggccggca gcctgctggc
     1681 tgagccgatg ccggaattca agctgcccaa cgagagcggc aaaagcgtga acctcaccga
     1741 actgctgggc cgtggccctg tcatcgtgga tttctgggcg gattactgcc agccctgcaa
     1801 acaggcgatg cctgccctgg acgccctcgc ccagaaatat gacagcctca ccgtggtgct
     1861 catttccatc gacgccccca aagcccaaac caaagccaaa aactacctca agggcaagaa
     1921 cttcaagttc gtctccctct tcgaccctga ccagaccctg gccaaaaagc tcaatgtagc
     1981 cgaaccgccc cacaccttca tcctggacaa ggccggcgaa atcgtgctgg aacacaaggg
     2041 cttcgtggct ggcgacgaaa aggaatatga gctcaaggtc cgctccctgc tgggctttga
     2101 agaggagatc gggcacgaat gcgattgcgc ggaaccttgc gaaaactgcg accataaaga
     2161 aaaaacggaa gccgctcccg accagaagcc ggcccccgaa aaagcccaca aaagtccatg
     2221 cggaaactgc cactgatctt cctggcggcg ctgctgctgg ccgggacagc cctgccggca
     2281 cagacctcgc tcacgctcaa cggtctcaac gaggccagcc tcatctaccg cagcgtgcct
     2341 gactccctgc acgtctattt ccagaacact ttcggcttca gcctcggcta caggaatttc
     2401 tctttcggga tgaagttcat cgccgaactg ccgaaataca gcaccaacca atccgaactg
     2461 ctggaaaatc tggccgccga ccgcctcagt cttggctgga aggagcttta cgcctcctat
     2521 gccaaagatg cctatctcat ccatgccggc atcatcgagg aaagttttgg ctccgggctc
     2581 attttccgca gctttatgga cgccgagctg gatgaagacc accgcgtgac cggcttcaaa
     2641 ttccgctacg acgacaagct tcgcctcaag gccctttaca gcgccctgcc cagccccacc
     2701 tcccacggca agctggacct ggcttacagc gcggatgccg aatatcctgt tctggagccc
     2761 ctcaccctcg gcgcttcctt tgtggggatg caaaccctgg tgggaactta ctacaaacag
     2821 gacgacatcc tgggggcgag ggcgtcgctg gttctggaca agtttgaact gtccgcggaa
     2881 ggcggcgtca gggatgacaa gctcgatgcc gggctgatgg acggtaaggc aatctacacc
     2941 gccgccaccg cctatcttgg ccctgtgcag cttgggggag cctttaagca ttacgacaac
     3001 tttgcctacc gcaaccgagt tcaggacctg cccctggcca atcaccacgg tgaagctttg
     3061 gccgacgacc tggcctccgg tgccgatgaa accggcttcc agggctgggc gaacatcgcc
     3121 atcggccagt cactctgcct cgctctggac tatgccgaag cctgggacaa agccggggaa
     3181 aaacagatga acgacgccta cgccggcttg gaatggcaaa gcggagccac tgccgccact
     3241 ctttcctaca gccacgtgga aaaggtggac agcgccctgc gccactggca aaaggaaagc
     3301 tatcccgcct tcgtggtcag cttccccgcc ggcaaagcgg gcctggttct ctccggcgag
     3361 ttcaagaccg tggaaaagct ggtgctggaa accgctcaga acgagtatgt cgaaattggc
     3421 cactacgaac cccgcctcca ggccgatctc agcctgggca agctcagcct ttccctgggc
     3481 gcgcaaagct ggtggaacga tttctcatcc atcctgaaaa gccactactg gccaagcgtt
     3541 gagctaaaat accccatcct cgcgaacagt gaccttgtcc tcttcgccgg caaggaagcc
     3601 gggggaaagg tctgccgcaa cggcgtttgc cgctatgtgg cccccttctc aggggtccgt
     3661 ttggaactcg ccacccgttt ttagatagga tgccattatg aaaaaaacga tatttatccc
     3721 gcttctgctc ctcctggctg ccctcctggc cgccgatcca aactactatc cgctcaccac
     3781 catcgcggaa tcctgtgtga ccgagaacag ccctgactgc gtcagcgccc tggccaacct
     3841 ggccaacgtg ctttccgcca cccatcgggg cgaattcatc gccacccggc tctatgatac
     3901 gggaaacctc agcagcccca gcgtggctga ccgcgtcgat cactacggaa tcatccagtg
     3961 gccgaccgtc attatgaacg gcacacacac cctgattggc gttcatccgg aaaccgtcta
     4021 tgcccaagcc gttgccgcca aatgctttca gccctcgccg ctgaaactcc agatcacttc
     4081 cttcactccc gccagcggcg ccgtctcggt ttccgccagg ctgctggacc acgatgtgaa
     4141 tatcaccggc caaaccctca tgctgatgct ggttgaggac aatgtgggtt ccgaaaccaa
     4201 cgtcgtccgc cagattaaat accagaccgt caacattccg ggctcaggcg atccggtgac
     4261 ctttacggac agcttttcca tcgattccac ttggaacagc gccaacctct gggcagtcgc
     4321 ggcggtgcat atgaatgacc atgcaattct tcagagcgcc tccaccctgc ccctgcccca
     4381 atacaacatc cgcgccgcca tgccctggaa ccctgccgag ctggtgacgc ctgccaattc
     4441 gtcgttgatc tcggaacctt tgtgggtctt caacaccggc agttctgaca acatgcagat
     4501 gcagctggtt gcggatgacg ctcctgacga ttggtatttc aactactgcg atgaagaggg
     4561 taactgctat cctggcagcg ggttgatgcc tctggaactg gccgccggcg aaagcaaggc
     4621 cttccatctc aatctttggg tcggttcacc aggcgtgggc agcttccatt ttgagataac
     4681 ttccgccaac ctcggcacct tcagcatccc cttcgtctgc caagccggaa cagaagtgtc
     4741 tgacgaagtg ctccaaccct cgctcagcct ggccggcaac agccccaatc ccttccgggg
     4801 cggcacgtct ttcacgctta acgcggagaa atcagtctcg gccagcatcc agattttcga
     4861 tgccaaaggg cgcctcgtag cggaaacacc tgcccagaac ctcgtccccg gctccaaccg
     4921 gatcgaatgg caggcccctt ccgaactgcc ttccgggata tatctttatc gcctcaaaga
     4981 gtcctccgcg cctccgcgcc ggatgctgct gatcaaataa gccatgagaa gatacctcgt
     5041 cctgttgctc ctgctggcag ccgtcgcttt ggccgccaca ccgctgggca aggagtttga
     5101 agaggccctg cggcccaccc aaaacgcgga ccaggccatg gaggtgatca ctacataccg
     5161 cgaccgcctc accgatctgg aagacctgcg ccagcttcag aactactgga tgcagatcga
     5221 ccccgatggc tgccgcgact ggtttggcca gcaatacaga gcccatccgg aaagccccga
     5281 gtttgaatac ctctggctgc gcggagatgc cgatcccggc acccaactgc ttgggggacg
     5341 ggcccttatc gcccgcgaac cctcattcta ctggggttac cgccttttca gcaacaccta
     5401 ttcccagctt ctccaggcag cgcctgattc aatccgtgcg gacataattg ccaacctcgc
     5461 cgccgaccgc gacatcctcc tccagggctt gaaaagatgg ccccaggacg actatctcca
     5521 cctcgccctc ttccatcatt atgcctccca gggcgaaaac gaacaagccg aagcacagct
     5581 cctcaacctc catgaacccg ccgccatcga agccaatttc cgccacgtga tcgaattcat
     5641 cggctcctcc ggcagcgtcc gcccctttga ggtcctctac ccgctcctca tttcccgcct
     5701 catcgccaaa ggcgagattt ccgccgccga cagtcttgac tactaccagc tatcctatct
     5761 ggaagccctc aaaaccgcca gggactggcc caaaatgtgc tctttcctgg aactcaaccc
     5821 cgggctccag acggatgaca ggaccctgga aacgcgcctg ctgatgtatc tggggctgga
     5881 acagccggaa acggcgctca accttctgga aggcgccctc gccgccggcg tcatcaccta
     5941 tccggaggct ctggacaacc cggattacgc tccactggct gaattgcccc gctatccgga
     6001 ggtgatggcc ctcgcctccg ccaattggga gaaggccaag gccgagcgca aggccaaaat
     6061 tatctcggaa aagacatccc gccccgcccc actatgggaa ttgcccgatc ccgacggcaa
     6121 gctgaaccgc ctcgaagatc tccgcggcaa gatcgttatc ctcgatttct gggctctctg
     6181 gtgctcaccc tgccttaaaa ccctgcccaa gctcgattcc tggctggaac ggaacccctc
     6241 cgacgacgtt gtgctgattt ccatcaacgt ttgggaaagt ccctccgaaa tacccaacgt
     6301 gattgaatac ttcagcaaga acagctacgg catgaagctc ctcctgggcg acaacgaact
     6361 gccccgggct tatggctttt cgggcatccc ctggctttgc gccatcgaca aacaaggcaa
     6421 catcgccttc acccaaagcg gcttcagccc cattctggat gaaattatct cggtctgggt
     6481 ggaggaactg cgtcgctgac ggggagttat gatgcggcgt ccaaatccct atctcacccc
     6541 caaggaacag cgcgcccttc tggtcctggc tgccctggcc ctgctcggtc tcgcggccgg
     6601 aaagcttaaa accgttcccg cttttgcccc cttcctccaa actgaacctg cctccccgga
     6661 actgctttca gtggccgccg aggtggacaa acccgtccag atcgacatcc gcaccgcctc
     6721 cctggaagaa ttgatgctcc tgcccggcat cggtcccaaa cgcgcccagg acatcatcga
     6781 ctaccgttcc caacatccct tcaccagcac cgacgacctc ctccagatca agggcatcgg
     6841 tgccaaaacc ctggccaaaa tgctcccttc ccttctgctc ttcggctcgc cccagttcac
     6901 tccagaagca ccttccgacc ccaactccat gaccaccatc ggcctgccgg accccggcca
     6961 aaacaacctg gcggaatccg atgcccccgc ctcatcctct tcagaaaaaa gctcttcccg
     7021 caaagccaaa cccaccccaa aaagccagct caccaatatt gtcaacctga acaccgccgg
     7081 cctcgctgaa ctctgcaccc tacctggcat cggagaggta aaagctcagg ccataatcga
     7141 ttaccgctcc caaaacggca agtttcaaag cgtcgatgac atcaccaaag ttaagggcat
     7201 cggtgccaaa accctcgcca aacttcgtca ccgcctcaag gtctgaactt tatagagtgc
     7261 cgaatagata gctttcttcg agaaaaaact tgtttcttaa tgtactcgcc ctgtttaggt
     7321 tgtatttgat ctccaaacca ggcgagtatt tcactcacag ccctaaaagc tcataattat
     7381 ggctctcttt caaaccgact gggtaatccg gtagatcggg cccgtagggc gatatatcga
     7441 tagggagaaa gcgtaagccc ctcgtacagt agcagtaacc agatggaaag ccccttgctg
     7501 gcgacaaata tcactcctca catttctgcc actatccggg ttgctttttg cttgaacacc
     7561 agcagccatt tgataaaaaa tagagccaaa actgcttgcc tgccgctatg tcctgtcagc
     7621 tacaccctta gcgctcgtgt ccatttccct ctctttcctc tcaccaaatc ctcgcatcaa
     7681 atgcggccat tatgcgggag gcacgtctgg gattcaggac aaggccttaa cggccagttg
     7741 gcttcgcttc cacacagcca cacgctggag ccgggcgaga gcggagcaat gaccacagcc
     7801 acaaccacat tatctgccca aactatcacg tgtgtcattc cgggaaaggg attcagcctc
     7861 agggccctgg aatccagttt tttgctcttt ctggatcccg gaagtcacct ctcgggctcg
     7921 gtgacgtccg ggatgatgtg gccttggagg ctggcgtcga gcgagaaaca agcatcgacg
     7981 ccagcaatga atgatttttc caatgcttcc tatcgtccat gctcactata ttcgctgtac
     8041 gccaggaaaa aaggcggtac agcaaccgcc agtaccatgt agcggcgctc gccgaagcgc
     8101 cgaaactccc aaacccaagt cattatcagc ttgacccgga atccagtgta cgagaggctt
     8161 acgcttcctc gctatcgata tgtcgcccta cgggcccgat ctaccggatt accgcccggt
     8221 ttgaaagaaa gcctgaaaaa agtgagaacc cacattataa actgacaccc aaagctgcaa
     8281 gaagtgacac aaggggaaag tggggataag gctttgattg gtggcgagat acgatggact
     8341 ggctttggaa atgtccaagc gcctatttgg acgtttttgg actgtttgct gacactatta
     8401 ctgcaaatta gcgaggcgtt tggacaggct ttggaaagag cagttggaaa aacggaactt
     8461 tgaaattaga aaaagcgcaa ttggagtgcg attgtgccga tcaggaaagc tccgtaattc
     8521 caatctgagt ctaaaaccag aaccgggtag tctatattaa atggttggag gataatctgt
     8581 tgcctgtggt ggtcgatctg cacctttttg atggtgaggc cgtcgtcagt ccttacaact
     8641 gcaattttac catcggcatt ttcccagctt agattgcttt taatgaccac gatgtcctga
     8701 tgctggagca caggttccat actcctgcca tttacttgaa agacagtata ttgcttgggg
     8761 ttaccgggga ggaatgatac tggcaactga atctgaccca tctgactcca ctcatccttg
     8821 atctccattg gcggtccggc tgcaatctca ccaactatgg ggaaggtgat cgaatgtgtg
     8881 ggatcgaagt ttttatctgt gatgggatgc aggattggag agattatggt ggggtttgat
     8941 acatctggat tggttgggct tgttgttgct tgattcgatt ggatcagctc ttggaactca
     9001 tccttggcat acatggaacc gtttccggta aagagccagt ggaggttgat ttggtctttg
     9061 gagagggctt ctaagaactc aggatcggga tagcgctcat tcagtttgta tcttgatagc
     9121 gaggcacggg agatgccata cttcttggca aactggtaat ccttgaggtg cattgcctta
     9181 atcaccaggc taaggcgttg tccgatagtc ggtttattca tcatggtctc catacagtaa
     9241 ctttactatt gactatatcc gcacggctgc aagattagtt ccagatgcca ttctgctgca
     9301 aggtggtttg gagtcaatac taaagattat agtgtgaccc aggtggatta taaagtattg
     9361 ccggagaatg cgtaagaaag ctggcaaact cgcagaaagg actt
//
LOCUS       DAOOWR010000019         9274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7382_length_9274_cov_4.267166, whole genome shotgun sequence.
ACCESSION   DAOOWR010000019 DAOOWR010000000
VERSION     DAOOWR010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7382_length_9274_cov_4.267166"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLB85_01065"
     CDS             complement(<1..1530)
                     /locus_tag="PLB85_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU71946.1"
                     /translation="MSRFSSKIITASAGTGKTYRLALEYIRIILDFYGKHQDFSLDNI
                     LVLTFTKKATAEIRERINSHLELLCNDKPATEEIANDRRGLLDSLWPEREGAELTDQD
                     LELLNRALRALSSDRRQLQVMTIDAYINSVFRNIVRPLRSIENFEIDIKAVEKRLPFL
                     LQHLMKPEFKVRLDSLLSRKISPSLDEYKHFFKSLIEQRWLHFQIRKAENKTPAEGTL
                     WWMFRDGGAEEPERLCESIRENLELLITLMAQNRPDLTPEECVVQKFRSLFPLFPDSY
                     AGLREAVARLCERPHGYLRLFQNCPEGKIASMSKFMRKDLTEVKQTLLYLQAQLNEDL
                     ADLLLHTHFLPEQEEILSVWSAILAEYDKLIYRYKNMTYDDISWFTLEALFSTDPPQF
                     DMQVEAVATEFYQFLAHRSRFILIDEFQDTSLIQFAILKPIIEEITSGEGTKDFGGMI
                     VVGDEKQSIFGWRGGERELLLNLRSIFGALRNLNQESLGTSYRSSEKMISFVNRVFKS
                     SL"
     gene            complement(1527..4406)
                     /locus_tag="PLB85_01070"
     CDS             complement(1527..4406)
                     /locus_tag="PLB85_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU71947.1"
                     /translation="MRTLINVPFSKDLIEAALGQVTDGCILVFPTRISAFKARRRFLE
                     GWALQDCDFVAMEDFKAALLLPPLPRISDDKRLLCLYQALSEEDRAYFHINGYFDIVE
                     WGGSFFQFFEELCDERVDSQILQDLPGRAQMTLLNWQEDYLNRVLEIRGRYRNILEEL
                     DFSDPIFSWEAAGVQVPYSGRKVVFVNQYYYSELEKALLDALEQAGNEVVALVQSQEE
                     KTRDLEPPRIELAGLKGENYNIRHLEIVECENAEQMVLAFLANHGQACGEDGEAVIVD
                     SHFSQSHYRDLFDPARFSCPRSESIFGSGIYQLLRVFKKHLEAMEATLEGAFLPLRLI
                     LDACAQESFLRYHGLAVKDKAPLLEELRYMLQKDILYVDRDLQLLDKLHREQGFPYLG
                     NILPKHFALLGQLSLISRPQDFIGLVDAPNGLRIKDLCREEELLHSDILDVFYERLTN
                     FASLETLGVVNDWKGLFGCEGTSLAAAVLQLFLESLGSGKFSFHQALKAEPRVEVSNL
                     LDLRNLQHHSVYFFHAIEGEIPSNPSPVWLLNENQRARLGLKSYPDLRERERYYFLRT
                     VLTSRRCVIFSYRDQENDIEPGSFVTELVQAMADGALPELARDGMAVKPTQLAPRISK
                     LYLAGHEMVTANAERLPGLADQAVCCVAKPSGDFFTLPCSPPEDFGAEGIVRTSYYSL
                     AMFRKNPFAWYLEHLRKLPELDLRPEETLTRKLFGSIMHSFLSAVLLPLAKEDADLPR
                     LERAFSDAKGLAATLNRILSDPDQLYLYKIPQNYNHEFLVSIISDNLVESIRVFYFEF
                     LKPRLQGVSFKLIPEEKFMTKEEKEGRKLVCVNHAGKEYSIWISGKADLRVECPELNY
                     IVDFKTGKGDDEQLIFYEWLYYLLDEAWAAKELRSLFWQVFDQKTGGRKITDKRRQEW
                     RGEQEDNLKQILEQGFTIGKKAGDRVKLASVTRADLFRADKGAEE"
     gene            complement(4704..5375)
                     /locus_tag="PLB85_01075"
     CDS             complement(4704..5375)
                     /locus_tag="PLB85_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71948.1"
                     /translation="MTGPGNQPLPLLELYQVLLSHNGCQNWWPAQTRDEVIIGAILTQ
                     SVAWGNVEKALVALAGQGILTLRDIHLADLQLLATLVRPTLYYNQKALRLKEFARFIK
                     ESCGFDLDILFSEELSSLRARMLSIKGLGPETVDSILLYAGGLPVFVVDAYTKRLLSR
                     LGYPIEKLDYHQLQDYICARIPAEVPLYNDFHAQIVLHCKDFCKKAPLCPDCPLRAVC
                     PSKTS"
     gene            complement(5476..5952)
                     /gene="ispF"
                     /locus_tag="PLB85_01080"
     CDS             complement(5476..5952)
                     /gene="ispF"
                     /locus_tag="PLB85_01080"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOU71949.1"
                     /translation="MFRVGQGYDVHTMAPGRKLILGGVRIPFHLGLLGHSDADVLVHA
                     VIDALLGALALGDIGSHFPDTDPAFAGADSRDLLRRVFAMVRERGWALANLDCTVCAE
                     RPKLSPYIEDIRDNLALDLNTSRDNVSVKATTEEGLGVSGGGAGMAAHCTLLLKRS"
     gene            complement(5956..6693)
                     /locus_tag="PLB85_01085"
     CDS             complement(5956..6693)
                     /locus_tag="PLB85_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71950.1"
                     /translation="MHILATKISGVAMEGALKIRETVLNHAEGREASEFKHGPNTIIG
                     KNTVYGFKHLRSFMRALSGVVENLYIKAEEQNISRRETRAINNALVDYIFTGNMPFNL
                     SPEADKLFRATVEGHNFFDSLYRNYPLIYVTGPDPRDVNLTISQINTHKIRGANTYVI
                     AEEDERLLKNASDNPREGGRYGWGYVMLPKTGDSLLTCFSATVALQMLALKMSLRKMK
                     KLDKLNIQDHGVHPDVPKNVSKSITVD"
     gene            complement(6694..8703)
                     /locus_tag="PLB85_01090"
     CDS             complement(6694..8703)
                     /locus_tag="PLB85_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOU71951.1"
                     /translation="MPRFFKLYNNALRKLAGFFKDIRLPLPILGLGCGVLGLALSKTT
                     LKLGEYASQLLRALEYRGFDSTGAAFQGEDENIVLLKDVGAPSTLVKTLGIEKQSGKL
                     FCGQVRWATFGNVDKTNAQPHEVKCKRHIYGAHNGNITNTRELKIWLSKEGHTVLSDN
                     DGEMLVHSVEHFFDIEMDKAGNPEDAELRKACMRRAIISTAERIVGSYAAVVVDPKTQ
                     TMWAIKAGSSLYFGVGELDGEPFKLASSDLTAVLRYTKMLVNLRDGEFVEYTSADYKV
                     YAQKKLRFKRVNAPDETYVAGDLIDTKPVLSKLRAEDVELQPEFEYFMEQEIFAQVES
                     TGKLVKLFQGGSNSGKRMLELIGQENLDELLNSKLKEILDCHDFDQRNMVFDALLASS
                     EFTNFFETAQANYRAIFEVAVQEDFEKKYFFSNDKNTLIDLIGPEYDIRRLILAKAFD
                     AVSESHSVQEFELSVQGFLDRVKNTIINSRNAYSIACGTSYNATKVGALFFNEIAKVE
                     IIPILPGDFRGGYSQCIKDNDLIIGVSQSGETKDLIDIFNDIEASNVKTSQVVLVNNL
                     NSTLGLEKGDLAIQILCGPEIAVPATKSFMNQITLIYYLAIRTARMKLEEGLDCEPEE
                     QRRKQLASLKAREQTLQQIPFLIKETLENTSDIVDSIAAQIYMDT"
     gene            complement(8715..>9274)
                     /locus_tag="PLB85_01095"
     CDS             complement(8715..>9274)
                     /locus_tag="PLB85_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU71952.1"
                     /translation="IERFPADTRMVYANPPFAPIGDYRRAVNAALDSPIDALPLEKQL
                     APSSRVTIAFDDPCLPVPLMLSDTRGVVIEELLKIVQKDKAVIPVARLKNTIKSIKGD
                     YIEQTIPRHNLVQVFTPQVFAYRLILAAYERAYQEGYVSTDDSALVEHNGGKVRYKFC
                     SDLNLKITDEVDLFFARQILDNNLI"
BASE COUNT         2012 a         2449 c         2599 g         2214 t
ORIGIN      
        1 cagggacgac ttgaacaccc gattgacaaa ggatatcatt ttctcgctac tgcggtagga
       61 tgtacccagg gattcttggt tcaggtttct cagcgcgcca aagatggaac gcaggttgag
      121 caaaagttcg cgttcgccgc cccgccagcc aaagatggac tgcttttcat cgcccaccac
      181 gatcatgcca ccgaaatcct tggtgccctc gccgctggtg atttcttcga tgatcggctt
      241 gaggatggca aactggatca gcgaagtatc ctgaaattcg tcgatcagga tgaagcggct
      301 gcggtgggcc aggaactggt aaaactcggt ggccacggct tccacctgca tgtcgaactg
      361 gggcgggtcg gtgctgaaca gggcttccag ggtgaaccag gaaatgtcgt cgtaggtcat
      421 gtttttgtag cggtagatca atttgtcgta ttcagccaga atggccgacc agaccgaaag
      481 aatctcttcc tgctcaggca aaaagtgggt gtggagcaac aagtcggcca ggtcttcatt
      541 aagctgggcc tgcaaataca gaagggtctg ttttacctcc gtcaagtcct tgcgcataaa
      601 tttgctcatg ctggcgatct tgccctcggg gcagttctga aacaacctca gatagccgtg
      661 gggcctctca cacagtctgg caaccgcctc acgtaaaccg gcatagctat ctggaaacag
      721 gggaaataag gaacggaact tttgcacaac gcactcttcc ggagtcaggt ctggcctgtt
      781 ctgcgccata agggtaatca atagttccag attttccctg atagactcgc aaagtcgctc
      841 aggctcttcg gcaccgccat ccctgaacat ccaccacagg gtgccctcag caggagtttt
      901 gttctctgcc ttcctgatct ggaaatgcag ccaacgctgt tcaataagag atttaaagaa
      961 atgcttgtac tcgtccaggg aagggctgat cttgcgactg agcaggctgt ccagcctgac
     1021 tttgaattcc ggtttcatca ggtgctggag aaggaaaggc agccgttttt ccacagcttt
     1081 gatgtcgatc tcgaaatttt cgatgctgcg caagggacgt acaatattgc ggaacacgct
     1141 gttgatatag gcatctatgg tcatcacctg cagttgcctg cggtcggagg aaagggcgcg
     1201 cagagcccgg ttcagcaatt caaggtcctg atcggtcagt tctgcgccct cgcgttccgg
     1261 ccaaaggctg tccagcaaac cgcgccggtc attggcgatc tcttcggtgg cgggtttgtc
     1321 gttgcaaagc aactccagat gggagtttat cctttcacgg atttccgcgg tggccttttt
     1381 ggtgaaggtg agcaccagga tgttgtccaa gctgaaatcc tggtgttttc cgtaaaagtc
     1441 cagaatgatg cgaatgtatt ccagggcaag gcgataggtt tttcctgtgc cggcgctggc
     1501 ggtgatgatt ttgctggaaa accggctcat tcttcagctc ctttgtccgc ccggaagagg
     1561 tcagcgcggg tcaccgaggc cagtttcacc cggtccccgg cttttttgcc gatggtaaat
     1621 ccttgttcca gaatctgttt caagttatct tcctgttctc cgcgccattc ctggcgacgt
     1681 ttgtccgtga tcttcctgcc tccggttttc tgatcaaaaa cctgccagaa cagggatcgc
     1741 agttccttgg ccgcccaggc ctcatccagc agatagtaca gccactcata gaagataagc
     1801 tgctcgtcgt cgcctttgcc agtcttgaaa tccacgatgt agttgagttc ggggcattcc
     1861 accctcaaat ccgcctttcc gcttatccag atagagtatt ctttgcccgc gtggttcaca
     1921 cagaccagct tgcggccttc tttttcctct ttggtcatga atttctcttc cgggatgagc
     1981 ttgaaggaaa cgccttgcag ccggggcttt aaaaactcaa aatagaacac ccggatggat
     2041 tcgaccaggt tgtctgagat gatgctgacc aggaattcgt ggttgtagtt ctgcggtatc
     2101 ttgtaaaggt agagttggtc aggatcgctc agaattctgt ttagcgttgc cgccaggccc
     2161 ttagcatcgg aaaaagcccg ttccagccgg ggcaggtcgg cgtcttcttt ggccaggggc
     2221 agaagcactg ctgagagaaa cgagtgcatg atgctgccaa agagtttgcg ggtgagggtt
     2281 tcctcaggcc gcaaatcaag ttccggcagt ttgcgcagat gctccagata ccaggcaaaa
     2341 gggtttttcc tgaacatggc caaactgtaa taactggtgc ggacaattcc ttccgcgccg
     2401 aaatcctcag gaggcgaaca gggcagggtg aaaaagtccc cagacggctt ggcaacacag
     2461 cagacagctt ggtcggcaag ccccggcagc cgctccgcgt tggctgtaac catctcatgg
     2521 ccagccagat agagcttgga aatccgcggc gccaactgcg ttggcttcac agccatacca
     2581 tccctggcca gttccggcag agcgccatcc gccatggctt gcacgagttc cgtgacaaag
     2641 ctgccaggct cgatgtcgtt ttcctggtcc cggtagctga aaatcacgca gcgccggctg
     2701 gttagcacgg tgcgcagaaa atagtagcgc tcacgttcac ggaggtcggg atagcttttc
     2761 aggcccaggc gcgcacgttg gttttcatta agcagccaaa ccgggcttgg attggagggt
     2821 atttcgcctt cgatggcgtg gaaaaagtaa acgctgtgat gctgcagatt tctcaggtcc
     2881 aaaaggttgc tcacctccac ccggggttcc gccttcaaag cctgatgaaa gctgaatttg
     2941 ccgctgccca gggattccag gaacagttgc agaactgccg cggctaaact cgttccctca
     3001 cagccgaaaa gtcccttcca atcattcacg acgcccaggg tttccaggct ggcgaagttg
     3061 gtcaggcgtt cgtagaaaac atccaggatg tcgctgtgca gcagttcctc ttcacggcat
     3121 aggtctttta tccgcagccc attgggagcg tcaaccaggc cgataaagtc ttgcggcctg
     3181 cttatcaagc tcagttggcc aagcagggcg aaatgtttcg gcaggatgtt tcccagatat
     3241 ggaaagcctt gctcgcggtg cagtttgtcc aaaagctgca agtccctgtc cacatagagg
     3301 atatcctttt gcagcatgta tcttagctct tcaagaagcg gtgccttatc tttaaccgcc
     3361 agaccgtgat agcgcagaaa agactcttgc gcgcaggcgt ccagaatgag ccgcagcggc
     3421 aggaaagcgc cttccagagt ggcctccatc gcctccaggt gctttttgaa aacgcgaagc
     3481 aactgataga tgccgctgcc aaagatgctt tcggagcgcg gacacgaaaa gcgggcggga
     3541 tcgaaaaggt cacgatagtg ggattggctg aagtggctgt ccacaatcac cgcttcacca
     3601 tcttccccgc aggcctggcc atggttggcg aggaaggcca gaaccatctg ttcagcgttt
     3661 tcgcattcaa cgatttccag atggcggatg ttgtaattct cccccttcaa gccggccaat
     3721 tcgattcggg gcggctccag gtcacgggtt ttttcctcct gggactgaac cagcgcgaca
     3781 acctcgtttc ccgcttgctc caaagcgtcc agcagcgcct tttccagctc gctgtaataa
     3841 tattggttca caaaaaccac tttcctgccg gaataaggaa cctggacgcc ggcagcttcc
     3901 caactgaaga tgggatcgct gaagtccaat tcttccaata tgttccggta gcgaccgcga
     3961 atctccagaa ctcggttcag atagtcttcc tgccagttca gcagcgtcat ctgggcacgg
     4021 ccgggaaggt cctgcaggat ttgactgtcc accctttcgt cacatagctc ctcgaaaaac
     4081 tggaagaaac tccctcccca ctccacgatg tcgaaatagc cgttgatgtg gaagtaggcg
     4141 cgatcctctt ccgacagcgc ctggtagagg cagagcaggc gtttgtcgtc actaatccgc
     4201 ggtagcgggg gcagcagcag agcggctttg aagtcctcca tggccacaaa atcgcaatcc
     4261 tgcaaggccc agccttccag gaagcgcctc cgggctttaa atgcggagat gcgggtggga
     4321 aaaaccagga tgcagccgtc ggtgacctgc cctaaagccg cttcgatcag gtccttactg
     4381 aaagggacat tgatgagggt gcgcaaagaa acctccacaa tagctttttc gcacgctcga
     4441 tccgggctgg ttttctgtca agccgaaatc ctttcaatct caatgacgga ggcttctgac
     4501 ccgcctggtt caactttcct gccttcttcc gcctcttaac aagccatact tgggacagag
     4561 gcggggcaag actggtggtt aaggcctgct ggttctgccc tcactcgcct cccgcaagat
     4621 ggccgcacct gatgcgggca ttgggcgggt ggttcaaagc gggcagattt gaaagggcta
     4681 aggaccagct cagctggggg cattcaggaa gtttttgagg gacatacagc cctcaaagga
     4741 cagtcggggc agagcggggc ctttttgcag aagtctttgc aatggaggac gatttgggcg
     4801 tggaaatcgt tgtagagggg cacctccgcg ggaatccggg cgcagatgta gtcctgaagc
     4861 tgatggtaat ccaacttttc aatgggatag ccgagacgcg acaaaagcct ttttgtgtaa
     4921 gcatcgacca cgaaaacggg caggccgcca gcataaagca gtatcgaatc gaccgtttcc
     4981 ggacctaacc ccttgataga cagcatcctg gcacgcaggg aggacagctc ctccgagaac
     5041 aggatatcca ggtcgaaacc gcaggattcc ttaatgaaac gggcaaactc cttgaggcgc
     5101 aaagccttct gattgtagta gagagtgggg cggacgaggg tggcaagcaa ttgcaaatca
     5161 gccagatgga tgtcccgcag ggtgaggatg ccctgtccgg caagcgctac caaagccttt
     5221 tcaacgttgc cccaggccac gctttgggtg aggatggcgc cgatgatgac ctcatcccgg
     5281 gtctgggcag gccaccagtt ttggcagcca ttatggctta acaggacttg gtaaagctcc
     5341 agcagtggga gtggctgatt gcccggtcca gtcatggttt gcaaataaat gatgtgacgg
     5401 ttcagcctcc gctgacgaag gctgtggcca gggtggcttt gctgttgtga tggaaacctg
     5461 tgattccggg cgctttcaag agcgcttcag caatagtgtg cagtgcgccg ccatcccggc
     5521 accaccgccc gaaacgccca aaccctcttc agtggtggct ttcacactca cgttgtcccg
     5581 gctggtattc aggtccaaag ccaggttgtc ccgtatgtct tctatgtagg ggcttagctt
     5641 ggggcgttcg gcgcaaacgg tgcagtcgag attggccagc gcccaaccgc gttcacgcac
     5701 catggcgaaa acgcggcgca gcaagtcgcg gctgtcggca ccggcaaagg cggggtcagt
     5761 atctggaaaa tgcgagccga tgtcacccag ggccagagct cccagcaggg cgtcgatcac
     5821 ggcgtgaacc agcacgtcgg catcggaatg acccagcagc ccaagatgaa agggaatccg
     5881 cactccgcca aggatgagtt tgcgtcccgg cgccatggtg tgaacatcgt aaccctggcc
     5941 aacgcggaac atggctcaat ccaccgttat tgatttggac acgttttttg gcacatccgg
     6001 atgcactccg tggtcctgga tgttcagctt gtccagcttc ttcatcttgc ggaggctcat
     6061 cttgagggca agcatttgca gtgccacggt ggctgaaaag caggtgagaa ggctgtcccc
     6121 ggtcttggga agcatcacgt agccccagcc gtagcggcct ccctcgcggg gattgtcgct
     6181 ggcgtttttc agcaggcgtt cgtcttcctc ggcgatcaca taggtgttgg cgccacgaat
     6241 cttgtgggta tttatttgag agatagtgag attcacgtcc cgcgggtctg ggccggtgac
     6301 atagataagc ggataattac ggtagaggga atcgaagaaa ttgtgtcctt caaccgtggc
     6361 gcggaatagc ttgtctgcct caggcgacag attgaaaggc atattgccgg tgaatatata
     6421 gtccaccagg gcgttgttga tggcccgggt ttccctgcgg ctgatattct gctcttcggc
     6481 cttgatgtac aggttttcca ccacgccgct taaagcgcgc atgaagctgc gcaggtgctt
     6541 gaatccgtaa actgtgttct ttccgatgat ggtgttcggg ccgtgtttga attccgaagc
     6601 ttcgcgtccc tcagcgtgat tgagaacggt ttcgcggatt ttgagggctc cttccatcgc
     6661 cacgccgctg atcttggtgg ccaggatgtg catttaagta tccatatata tctgagccgc
     6721 gatggagtca acgatatcgg atgtgttttc cagggtttcc ttgatcagga agggaatctg
     6781 ctgcagcgtc tgttcccggg ctttaaggct ggccagttgt ttccggcgct gttcttcggg
     6841 ttcgcaatcc agtccttcct caagtttcat cctggcggtg cggatggcca gatagtaaat
     6901 cagggtgatc tggttcatga agctcttggt ggcgggcact gcgatttctg gtccgcagag
     6961 gatttggatg gcgaggtcgc ctttttccaa tcccaaggtg gagttcaggt tattcaccaa
     7021 caccacttgg gaagttttca cgttgctggc ctcaatgtcg ttgaagatgt cgataaggtc
     7081 tttggtctca ccggattggg agactccgat gatgaggtcg ttatccttta tgcattggga
     7141 atatccgccg cggaaatcgc ccggaaggat gggtatgatc tccactttgg cgatttcgtt
     7201 gaaaaagagg gcgccgacct tggtggcgtt gtaactggtg ccgcaggcga tggaataggc
     7261 attgcggctg ttgattatgg tgtttttcac ccggtccaga aatccctgca cgctgagctc
     7321 gaattcctgc acgctatgac tttcggaaac cgcgtcgaaa gctttggcca ggataagccg
     7381 ccgaatgtca tactccgggc cgatcaggtc gatgagggtg tttttgtcgt tggaaaagaa
     7441 gtacttcttt tcaaaatcct cctgaacggc aacctcaaaa atagcgcggt aattggcttg
     7501 ggctgtttcg aagaaattcg tgaactcgct ggaggcaagc agggcatcaa agaccatgtt
     7561 ccgttggtca aaatcatggc agtccaagat ttctttcagc ttggaattga gcagttcatc
     7621 cagattttct tggccgatga gttccagcat gcgtttgcca gaattcgatc cgccttgaaa
     7681 cagcttcacc agcttgccgg tggattccac ctgggcgaaa atctcctgct ccatgaagta
     7741 ttcaaattct ggctgtagtt ccacgtcttc ggcgcgaagc ttggaaagca ccggcttggt
     7801 atcgatcagg tcaccggcca cgtaggtttc atcaggtgcg ttcacacgtt tgaagcggag
     7861 tttcttttgc gcgtaaacct tgtaatccgc ggaggtgtat tccacaaact cgccatcgcg
     7921 cagattcacc agcatcttgg tatagcgcag aaccgcggtg agatcggaag aagccagttt
     7981 gaagggttca ccgtcaagct cgcccacccc gaaatagaga ctgctgccgg ctttgatggc
     8041 ccacatagtc tgggttttgg gatccaccac caccgcggcg taggaaccaa cgattctttc
     8101 cgctgtgcta atgatggccc ggcgcatgca ggctttgcgg agttccgcat cctcgggatt
     8161 tccggctttg tccatctcga tatcgaaaaa gtgctccacg ctgtgcacca gcatctcgcc
     8221 atcgttgtcc gaaagcacgg tgtggccttc tttgctaagc cagatcttga gttcacgggt
     8281 gttggtgata ttgccgttgt gggcgccata gatgtggcgt ttgcatttca cctcatgggg
     8341 ttgggcgttg gttttgtcca cgttgccgaa ggtagcccag cgcacctgtc cgcagaacag
     8401 cttcccactc tgcttctcga tcccgagggt cttcactaag gtgctgggag ctcccacatc
     8461 cttgagcaga acgatgtttt catcctcacc ctggaacgct gcgccggtgg aatcaaaacc
     8521 ccggtattct aaagcccgta gcagttgaga agcgtattct cccagtttca gagtggtttt
     8581 ggacaacgcc aggcccagaa ccccgcaacc caatcccagg atgggcagcg gcaaccggat
     8641 gtccttgaaa aagcccgcaa gcttgcgcag ggcattatta taaagcttaa aaaagcgtgg
     8701 cataattact tcctttagat aagattgttg tccaggatct gccgggcgaa gaacagatcc
     8761 acttcgtcgg tgattttcag attcaggtct gaacagaatt tatagcgaac tttaccacca
     8821 ttgtgttcca ccaaagcgga atcatcagtg ctcacatagc cttcctggta ggcgcgctca
     8881 taggcagcca ggatcaaccg gtaggcaaaa acctgaggcg tgaatacctg caccaggttg
     8941 tgacggggaa tggtttgttc aatataatcg ccttttatgc tcttgatggt gttttttagc
     9001 ctggcaacgg ggataacggc cttgtccttt tgcacgattt tcagcagttc ttcgatcacg
     9061 acccccctgg tgtcgctcag catgagggga accggaaggc aggggtcgtc gaaggcaatg
     9121 gtgaccctgc tcgatggggc cagctgcttt tccaggggaa gggcgtctat cgggctgtcg
     9181 agcgccgcgt tcacggccct tctgtaatct ccgatgggcg caaacggcgg gttcgcatag
     9241 accatgcggg tgtcggcagg aaagcgctcg atcc
//
LOCUS       DAOOWR010000020         8941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_7782_length_8941_cov_3.831420, whole genome shotgun sequence.
ACCESSION   DAOOWR010000020 DAOOWR010000000
VERSION     DAOOWR010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7782_length_8941_cov_3.831420"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /gene="argS"
                     /locus_tag="PLB85_01100"
     CDS             complement(<1..1616)
                     /gene="argS"
                     /locus_tag="PLB85_01100"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU71953.1"
                     /translation="MIKHLLHDNIREAISTLELSSSRDFTVEVPNNPENGDYSTNAAM
                     VLARENKTAPKKLAEKLTKELRRNKFYKTVEIAGPGFINFRLSASLFQKMLWDIHEAG
                     AKFGSSDYGQGIKVLLEFVSANPTGPLNIVNARAAAFGDTLYRVMKKVGYEPAREFYV
                     NDAGNQVDILAESLELRLREIHGENIGEFPYEAYHGEYVKHLAHKLNAAEGVRIFMMP
                     EKERTERLKEFALNELLEMQRLSLEKFDVFFESWVSEKTLRAEGVVEEVLSYLTEADC
                     TYEKEDAIWFSSTKFGDDKDRVLMKSDGSITYFVPDLAYHLTKIQRGYTQLIDVFGPD
                     HHGYVPRLKAAFRALKYDEDMLEIIFLQQVNLFESGERVKMSKRAGKIVTMDDLVNVV
                     GKDAARYFFIARKANAHLNFDLELALQQNNENPVYYCQYAHARICSILKKARKDKVYP
                     RSFKPELTHKLNKPDEMALIQKLADLPELLQLIAQHREPHRLATYCEELCSLFHRFYN
                     KYKVVSAKNKELSQARLLLIETVKNVLAICL"
     gene            complement(1594..1965)
                     /gene="rsfS"
                     /locus_tag="PLB85_01105"
     CDS             complement(1594..1965)
                     /gene="rsfS"
                     /locus_tag="PLB85_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU71954.1"
                     /translation="MADNAKLEAVLTWLAEKQAENIRVYDVGKNSAYTDFIVVCEGTA
                     DLHNKAIANHVLDMAKEHKLLVLSKAGLEFGVWVLLDISDVVIHIFLPEKREYYKIDE
                     FSSELAGLSDKEKPHDQAPTA"
     gene            complement(1980..2414)
                     /locus_tag="PLB85_01110"
     CDS             complement(1980..2414)
                     /locus_tag="PLB85_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOU71955.1"
                     /translation="MNRRTKTNSMKMTLLWLAVGLLGLVLGYLVYIQLIVPGRNTDSL
                     KEENLPALKVVVKNGCGVENLVTEYSDYIKDENIDVISMGDTPQPIWSKSVIEVKTDD
                     RQDLARLQKMTGIKRYTLAVDPDAPAPFVIILGQDLEEYMKP"
     gene            complement(2428..2928)
                     /locus_tag="PLB85_01115"
     CDS             complement(2428..2928)
                     /locus_tag="PLB85_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOU71956.1"
                     /translation="MTDGYLYSPWRLDYIQGVKPEDCILCRFLESEKDAENLIVFRGE
                     HCYVMLNRYPYNNGHIMLVPYLHTKILRELPPEVMNELGKLLQRSEQVLIQSYNCDGI
                     NIGINLGRAAGAGIDEHLHIHLVPRWNGDCNFMSVVGGKRVIPEAFEISYKRLRDAYA
                     KLTEQE"
     gene            complement(2921..3949)
                     /locus_tag="PLB85_01120"
     CDS             complement(2921..3949)
                     /locus_tag="PLB85_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU71957.1"
                     /translation="MKEPAAIILAAGKGTRMKSNRAKVTFPIADKPMVQRVADSAAKA
                     GCARICVVVGYQKESVIACLEDDKRLEFVEQSEQLGTGHAVMMARPLFTDSDQDVLIL
                     SGDVPLLSSETLMKIHQSHQASGSACTVLTAILDDPGKYGRILRDAEGQISGIVEFKD
                     ASEAQRSIKEWNTGIYCFRSGDLFSALDQISNTNQQQEYYLTDVVSILRKAGKKVAGV
                     VLENLVEVSGVNSQEQLAALEDQFLDSIRKRWLNNGVVIHNPQTVFIGDDVILEPDAE
                     ICQGSVIKGNSQICTGAHIGPGCYLADSFVSDNAILEGHNVLVDAHIPEGHILEFGEQ
                     VIEETFND"
     gene            complement(3946..4419)
                     /gene="folK"
                     /locus_tag="PLB85_01125"
     CDS             complement(3946..4419)
                     /gene="folK"
                     /locus_tag="PLB85_01125"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU71958.1"
                     /translation="MTAWLCLGSNQMKPRAQVQKAVRILATDPKIRVLRKSSLVRTKA
                     YGKEDQPDFRNQVIEIDTKYSPLDLLQKGLVVETEMGRVRKERWGPRVIDIDILLYED
                     EEIQTMSLTLPHPDFHNRRFVLELLCEVDPELKHPVLNKTMAKLLEELGHAGGEQ"
     gene            complement(4416..4775)
                     /gene="folB"
                     /locus_tag="PLB85_01130"
     CDS             complement(4416..4775)
                     /gene="folB"
                     /locus_tag="PLB85_01130"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU71959.1"
                     /translation="MTIRLNEMVFYGYHGVHDEERKLGQRFIVSLEMQTRPETDTAIR
                     QLEDTVDYTKVYAQIREIMESQQFQLLEACANILADKLLENFPLIWQLTLRIQKPSVP
                     IKGILRSVEVELTRSRA"
     gene            complement(4772..5191)
                     /locus_tag="PLB85_01135"
     CDS             complement(4772..5191)
                     /locus_tag="PLB85_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU71960.1"
                     /translation="MSKNTKIAQEDVGTVVAVNSGRVKVELVRGGGCKSCSMQGICFG
                     RNTPAVFDLESDLDLNPGDRVELEIAASTRVLTSLLVFGLPMLCLFTGFLVASHWLAE
                     LPAIAVAFGVTALSFLIMRQIDKRYGNRMQVRIRRKI"
     gene            complement(5188..5676)
                     /gene="rfaE2"
                     /locus_tag="PLB85_01140"
     CDS             complement(5188..5676)
                     /gene="rfaE2"
                     /locus_tag="PLB85_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOU71961.1"
                     /translation="MIKDKIKSWEEAAFLTEELRAQRARIVFTNGCFDLLHSGHTQYL
                     EQAKALGDILIVGLNSDASVSLLKGESRPFVAQTDRALVLAALESVDMVVIFSQPTPL
                     QLIELLRPDVLVKGGDWKPKTIVGADIVLAGGGKVLSLPFKEGYSTSSLIRRIAATEV
                     QK"
     gene            complement(5663..6634)
                     /gene="rfaE1"
                     /locus_tag="PLB85_01145"
     CDS             complement(5663..6634)
                     /gene="rfaE1"
                     /locus_tag="PLB85_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOU71962.1"
                     /translation="MIGELLQKFDGQRVLVLGDVMLDQYVWGRVDRISPEAPVPVLEA
                     QSEELRLGGAANVALNIRTLGGIPLLVGITGADEAARELRSLLEARGISAEGLISDPK
                     RSTTRKMRIGAANQQIVRIDYENKEEVGAELQKKVLSKLKTLLPDCLALIIEDYDKGL
                     LTIGLIRQALKLASERGVPVAVDPKLKHFQAYAGVDILKPNYKELQDVSGTVFCTDEE
                     FFQTAQKLRHEMRIRHLIVTRSSLGMYIFSGDDAPKHIPTFAREVFDPSGAGDTVISA
                     LTLAWISGADIDLAAELANHAAGVVCGIKGTASVSPTQVLESYHDKR"
     gene            complement(6631..7998)
                     /gene="purF"
                     /locus_tag="PLB85_01150"
     CDS             complement(6631..7998)
                     /gene="purF"
                     /locus_tag="PLB85_01150"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU71963.1"
                     /translation="MCGIVGVFGNPEAASLAALGMFAEQHRGQESCGMAVCDGKVIRL
                     HKSMGLVKEVFTPEVLESLPGSIAIGHVRYPTKGSATAFNTQPHLVETLFGPSYALAS
                     NGDVVNYASVRAKLEAKNVYFKSDNDGELLVKFIAWRIDQGDEPADAIRALMREIKGA
                     YSCVFTTRRELYLFRDPLSMRPMVWGKTSNGTVVVASESCALDILQVNGRQEVPPAGI
                     IRVNKDGISLLENDPNLYRSRRHNSYCVFEHIYFSRPDSYHFGENVFAVREKIGELLA
                     KDDEGLQADCVVPVPDSSNLMAVGYSAASGVPLSLGLIRNHYIGRTFIKPQQTIRDEG
                     VYQKFNPLPNFYKGKKVVLVDDSIVRGTTIRKIVKMIKEAGAEEIHLRIGSPQVKFSC
                     YFGIDTPHPEELAANRHSLEELRTWTGVDSLKHLSLASLAQCLTKPEEYCYACFDGNY
                     PLEME"
     gene            complement(7998..8660)
                     /gene="plsY"
                     /locus_tag="PLB85_01155"
     CDS             complement(7998..8660)
                     /gene="plsY"
                     /locus_tag="PLB85_01155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOU71964.1"
                     /translation="MIQAVIWVLVCLLAYLIGSIPFGWLAGKFFHKKDIRSGGSGNIG
                     ATNALRQYGVKTGILVLALDLFKGFAAAWLLLNVVPGLVSNFAGALPTERNMLLLPAL
                     AVILGHMFPVWLNFKGGKGVATAAGVYLVLAPVPLFAALLVFLLVTALSRYVSLGSIV
                     AAASLFVCELLWNIFVLREPEFPWFTLFVALLVIYKHSQNIMRLLEGRENRLSFNKKD
                     KA"
     gene            complement(8657..>8941)
                     /locus_tag="PLB85_01160"
     CDS             complement(8657..>8941)
                     /locus_tag="PLB85_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU71965.1"
                     /translation="ESEKRISTSELNRFMETVVQHRPPTHSTGRHVKIYYLTQAAVKP
                     PTFVFFCNQPSLVSENYKRFLHNQIREMFSFEGVSIKLIFRGRKDGETEQ"
BASE COUNT         1892 a         2438 c         2390 g         2221 t
ORIGIN      
        1 aggcagatgg ccaaaacgtt cttcacggtt tctatgagca gcagccgggc ttgtgagagt
       61 tccttgttct tggcgcttac caccttatac ttgttgtaga aacggtggaa gaggctacag
      121 agttcctcac agtaagtggc caaacggtgg ggttcgcggt gctgggcgat cagttgcagc
      181 aattcgggca gatcagctag tttctggatc agggccattt cgtccggttt gttcagtttg
      241 tgggtgagtt ccggtttgaa agagcgggga tagaccttgt ccttgcgcgc tttcttgaga
      301 atagagcaga tgcgggcgtg ggcgtactgg caatagtaaa caggattttc attgttctgc
      361 tgtaaagcca gctcaaggtc gaaattcaga tgggcattgg ctttgcgggc gatgaagaaa
      421 taccgggcgg cgtctttgcc aaccacgttc accaaatcgt ccatggttac tatcttgccg
      481 gcgcgcttgc tcattttcac gcgctcaccg ctttcgaaga gattcacctg ctgcagaaag
      541 atgatttcca gcatgtcttc atcgtatttg agggcccgga aggctgcctt gagccggggc
      601 acatagccgt ggtggtcggg tccgaagaca tcgatcagct gggtgtagcc gcgctggatc
      661 ttggtgaggt gataagccag gtccggcacg aaataggtga tggagccgtc acttttcata
      721 agcacgcggt ctttgtcatc accgaatttt gtggaggaaa accagatggc gtcttccttt
      781 tcataggtgc aatcagcttc cgtaaggtag ctgagcactt cttccaccac gccttcagcg
      841 cgcaatgtct tttccgagac ccagctttcg aagaagacgt caaacttttc taggctgagg
      901 cgctgcattt ccagcaactc gttgagggca aattctttca ggcgttcggt gcgttccttt
      961 tcaggcatca tgaagatgcg cactccttca gcggcgttca acttgtgggc caggtgtttc
     1021 acatattcgc cgtggtatgc ctcatatgga aactcgccga tgttttcacc gtgaatctcg
     1081 cgcagacgga gttcaaggct ttccgccagg atatccacct gattgccggc gtcattcaca
     1141 taaaactcgc gggcaggctc ataacccacc tttttcatca cccgatagag ggtgtcaccg
     1201 aatgccgcgg cgcgggcgtt gactatgttg agcgggccgg tggggttggc gctcacaaat
     1261 tccagcaata ccttgatgcc ctggccgtaa tccgagctgc caaacttcgc gccggcttcg
     1321 tgaatgtccc aaagcatctt ctggaaaagg gaggcggaga gccggaaatt gataaaaccg
     1381 ggcccggcga tctcgaccgt tttgtaaaac ttgttcctgc gcagctcttt cgtcagtttt
     1441 tcagccagtt ttttgggcgc ggtcttgttt tccctggcca aaaccatggc ggcgttggtg
     1501 gaataatctc cgttttcagg attgttgggt acttccaccg tgaaatcgcg gctggagctg
     1561 agttccagag tgcttattgc ttcccggata ttgtcatgca gtaggtgctt gatcatgtgg
     1621 tttctcttta tcgctgagac cggccagttc gctggagaac tcgtcgatct tgtaatattc
     1681 tcttttttca ggcaggaaga tgtggatcac cacatcgctg atgtccagca gcacccaaac
     1741 gccaaattca aggccagcct tgctcagcac caggagcttg tgttccttgg ccatgtctaa
     1801 aacatggttg gcaatggcct tgttatgcag gtccgcggtg ccttcgcaca ccacaatgaa
     1861 atccgtgtag gcgctgtttt tgccaacatc gtaaacccgg atgttttccg cttgtttttc
     1921 cgcgagccag gtgagcacgg cttcgagctt tgcattatcc gccaaattgt tctccttgtt
     1981 catggtttca tgtactcttc aagatcctgt cccagaataa tcacgaaagg ggcaggggcg
     2041 tcagggtcca cagccagcgt gtagcgcttg atgccagtca ttttttgcag gcgggcgaga
     2101 tcctgcctgt cgtccgtttt cacttcgatc acgcttttgc tccagatagg ctggggcgta
     2161 tcgcccatgc ttatcacatc gatgttttca tccttgatgt aatccgaata ttccgtgacc
     2221 aggttttcca cgccacagcc gtttttcacc actaccttga gggcgggtag attttcttct
     2281 ttcaggctgt ctgtatttct gccgggaacg atgagctgaa tatagaccag atagcccagc
     2341 accaggccaa gcaaacccac cgccagccac agcagggtca ttttcatcga gttggttttg
     2401 gtacgacggt tcaagttggc cccaaagcta ttcctgctca gtaagtttcg cgtaggcgtc
     2461 gcgcaggcgt ttgtagctga tttcaaaggc ttccgggatc acgcgttttc cgccaaccac
     2521 gctcataaag ttgcaatcac cgttccaccg gggaaccaga tggatgtgca gatgttcgtc
     2581 tataccggcg ccggcggccc ggcccagatt gatgccaatg ttgatcccat cgcagttata
     2641 ggattggatg agcacctgtt cgcttctctg gagcagcttg ccgagttcgt tcatcacttc
     2701 ggggggcagt tctctcaaga tttttgtgtg cagatagggc accagcatga tgtggccgtt
     2761 gttgtaggga tagcggttga gcatcacgta gcagtgttcg ccgcggaaaa cgatcaggtt
     2821 ttcagcgtct ttttctgatt ctagaaaacg gcagaggata cagtcttcag gttttacgcc
     2881 ctggatatag tccagccgcc agggggaata aagataaccg tcagtcattg aatgtctctt
     2941 ctatcacttg ctcgccaaat tccaagatgt ggccttccgg aatgtgggcg tcaaccagaa
     3001 cgttgtgccc ttccaggatg gcattgtcgc taacgaagct atcggccaga taacagccgg
     3061 ggccaatgtg ggcgccagtg cagatctggc tgttgccttt gatcacggag ccctggcaga
     3121 tttccgcgtc cggttccaag atcacgtcgt cgccaatgaa cacggtttgc ggattgtgga
     3181 tcaccactcc gttgttcagc cagcgtttgc ggatggaatc caagaattgg tcttcaaggg
     3241 cggccagttg ttcttgggaa ttcactccgg acacctccac caggttttcc agcacgacgc
     3301 cggccacttt tttccctgcc ttgcgcagga tggaaacaac atccgtgagg taatattcct
     3361 gctgctggtt cgtgttcgat atctggtcca gagcactaaa gaggtcacct gagcggaagc
     3421 aatatatgcc cgtattccat tctttgatag agcgttgcgc ttccgaggca tctttgaatt
     3481 ccacgatgcc gctgatctgc ccttccgcgt cacgcaggat gcgtccgtat ttaccaggat
     3541 cgtccagaat ggcggtaagg acagtgcacg cggagcctga agcctgatgg gactggtgta
     3601 ttttcatcag ggtctccgaa ctgagcaggg gcacatctcc gctaaggatc agcacgtcct
     3661 ggtcactgtc tgtaaacagc ggccgggcca tcatcacagc gtggccggtg cccagttgtt
     3721 cactttgttc cacaaattcc aggcgtttgt cgtcttccag gcaggcgatc acgctttcct
     3781 tctgataacc aaccaccacg cagattctgg cgcaaccggc cttcgcggcg ctgtcggcca
     3841 ctctttgcac catgggctta tccgcgatgg ggaaggtcac cttagcccgg ttggatttca
     3901 tcctggttcc tttgccggct gccaggataa tcgcggcagg ttctttcatt gctctcctcc
     3961 agcgtggcca agctcttcaa gtaacttagc cattgtttta ttcagcaccg ggtgcttgag
     4021 ttcagggtcc acctcgcaca gcaattccag cacgaagcgg cggttgtgaa agtccggatg
     4081 cggcagggtg agggacatcg tttgtatctc ttcgtcctca tacagcagga tgtcgatatc
     4141 gatcactcgg ggcccccagc gttccttgcg cacgcggccc atctccgttt ccacgacgag
     4201 gcctttctgc aaaaggtcca ggggcgaata tttggtgtcg atttctatca cctgattgcg
     4261 gaaatccggc tggtcttctt tgccgtaggc tttcgtgcgc accaacgagg atttgcgcaa
     4321 tactcgaatc ttgggatcgg tggcaagtat tctcaccgct ttttgcactt gggcgcgggg
     4381 tttcatctga tttgacccga ggcacagcca ggcggtcatg cacgactcct tgtcagttca
     4441 acttccacgc tgcgcaggat tcccttaatg ggaacggagg gtttctgaat gcgcagggtg
     4501 agctgccaaa tcaacggaaa attctccaac agcttgtccg ccaaaatatt ggcacaggct
     4561 tccagcagct ggaactgctg agattccata atctcgcgga tttgggcata gaccttggtg
     4621 tagtccaccg tgtcttccag ttgccggatg gcagtatcgg tttcagggcg ggtctgcatt
     4681 tccaggctca cgatgaagcg ctggccaagc ttgcgttctt cgtcgtgaac gccgtgatag
     4741 ccgtaaaaaa ccatctcgtt cagtctgatg gtcatatctt tctcctgatc cgcacctgca
     4801 tccggttacc gtatcttttg tcgatctgtc tcattattaa aaaactaagc gccgttacgc
     4861 caaaagcaac ggcgatggcg ggaagttcag ccagccagtg gctcgcgacc agaaatccag
     4921 tgaacaaaca gagcataggc aatccaaaca ccaataacga cgtcagcaca cgggttgagg
     4981 cggcaatttc caactccact ctatctccgg gatttaggtc cagatcgctt tccagatcga
     5041 acaccgccgg ggtgttgcgc ccgaaacata ttccctgcat ggagcaggat ttgcagccac
     5101 cgccgcgcac cagttccact ttcacacggc cactattcac ggccacaacc gtgcccacgt
     5161 cttcttgggc gatcttggta tttttgctca tttctgtact tccgttgccg cgatccgccg
     5221 aatcagcgaa ctggtggagt atccttcttt gaaaggcagg ctcaacacct tgccgccacc
     5281 ggccaaaaca atatctgcgc ccacgatggt cttaggtttc cagtctccgc ccttaactaa
     5341 aacgtctggt ctgagcagtt cgatcagttg aagcggagtg ggctggctga aaatcaccac
     5401 catgtccacg ctttcgagag cggccagcac caaagcgcgg tcggtttggg ctacaaaggg
     5461 tcgcgattct cctttcaaca ggctcacgct ggcatcgctg ttcaggccaa cgatcaggat
     5521 atcgccaagg gctttggcct gttccagata ctgggtgtgg ccggagtgca ggagatcgaa
     5581 acagccgttg gtaaatacaa tccgggctcg ctgagctctc agctcttcgg tcaggaaggc
     5641 tgcttcctcc cagcttttga tcttatcttt tatcatggta gctttccaaa acctgggtcg
     5701 gtgaaacgct cgctgttcct ttaattccgc aaaccactcc ggcggcatgg tttgccagtt
     5761 ctgcggccag atcgatatcg gcgccactaa tccaagccag ggtgagggcg gaaatcaccg
     5821 tgtcgccggc gccggagggg tcaaatacct ctcttgcgaa agtgggtata tgcttcggag
     5881 cgtcatcacc gctgaaaatg tacatcccca gactgctacg ggtcacgatc agatgcctga
     5941 tccgcatctc atgccgcaat ttctgggctg tctgaaaaaa ttcctcgtca gtgcagaaaa
     6001 cggttccgct aacatcttgt agctctttgt aattgggctt taatatgtcc accccggcat
     6061 aggcttggaa atgtttcagt ttggggtcca ctgccaccgg tacaccccgt tcggaagcca
     6121 gcttcaaagc ctggcggatc agaccgatgg ttagcaaacc tttgtcataa tcttcgatga
     6181 tcagggctag gcaatccggc agcagagttt tcaacttgga caaaaccttt ttctgcagtt
     6241 cagcaccaac ttcctctttg ttttcgtagt caatgcgcac gatctgttga ttggctgccc
     6301 cgatgcgcat cttgcgggtc gtggaacgtt tgggatcgct tatgagtccc tcggccgaaa
     6361 tgcctctggc ctcaagcaag ctcctcagtt ccctggcagc ttcgtcagct cccgtgatac
     6421 cgaccagaag tgggattccg cccagggtgc ggatgttcag agccacattg gcggctccgc
     6481 ccaggcgcag ttcctcgctt tgggcttcca gaacgggtac cggcgcttcc ggtgaaatgc
     6541 ggtccaccct gccccacaca tattggtcca gcattacgtc gcccagcacc agaacgcgct
     6601 ggccatcgaa tttttgcagt aattcgccga tcattccatc tccaggggat agtttccgtc
     6661 aaagcaggcg tagcagtact cttcgggttt ggtcaaacat tgggccagac tggccaagga
     6721 caagtgtttc aggctgtcca ctcctgtcca ggttcgtagc tcttccaggc tgtgacggtt
     6781 ggcggccagt tcctcggggt gcggggtgtc gatcccgaag tagcaggaaa atttcacttg
     6841 gggagaacca atgcgcagat ggatctcttc cgctccggct tctttgatca tcttcacgat
     6901 tttgcggatg gtggttccgc gcacaataga atcgtccacc agcaccacct ttttcccttt
     6961 ataaaagttg ggcagagggt tgaatttctg gtaaacaccc tcgtcgcgga tggtttgctg
     7021 aggcttgatg aaagtacggc cgatatagtg gttgcggatc aggcccagcg agagcgggac
     7081 gccgctcgcg gcggagtaac ccacggccat taaattagat gagtccggca ctggcacaac
     7141 gcagtctgcc tgaaggccct catcgtcttt ggccagcaat tcgccgatct tttcgcgcac
     7201 cgcgaagacg ttttcgccga aatgatagct gtccggccgg gagaaataga tgtgttcgaa
     7261 aacgcaatag ctattgtggc gacggctgcg gtaaaggttg ggatcgtttt ccaggaggga
     7321 gattccgtct ttgttaacgc ggattatccc agccggaggc acttcctggc gtccgttcac
     7381 ctgcaagatg tccagcgcgc agctttcaga ggccacaacc acagttccgt ttgaggtttt
     7441 accccacacc atgggacgca tggaaagcgg atcgcggaaa aggtaaagct ctctcctggt
     7501 ggtaaatacg caggaatagg cgcccttgat ctcgcgcatc aaagcccgga tggcgtcggc
     7561 tggttcgtcg ccctggtcga tgcgccaggc aatgaatttc accaacaatt cgccgtcatt
     7621 gtcgctcttg aaataaacgt ttttcgcttc cagcttggcc ctgactgaag cgtagttcac
     7681 cacgtcgccg ttgctggcca gcgcgtagga cggcccgaac agcgtttcca ccaggtgggg
     7741 ctgggtattg aaggctgtgg ctgaaccctt ggtgggatag cgcacatggc cgatggcaat
     7801 gcttcccgga aggctttcca atacttcggg agtgaaaact tccttcacca ggcccatgct
     7861 tttgtgcagc cgtatgacct tgccgtcaca aaccgccata ccgcagcttt cctggccgcg
     7921 gtgctgctcg gcgaacatcc ccaaagcggc caagctggct gcttcaggat taccgaaaac
     7981 tccgactatt ccacacatca ggctttatcc ttcttgttaa aactcagacg gttttccctg
     8041 ccttccagca ggcgcatgat gttctgactg tgcttatata tcaccagcag agccacgaac
     8101 agcgtaaacc agggaaactc cggctccctg agcacaaata tgttccacag caattcacac
     8161 acaaaaaggg aggccgcggc aacgatcgag cccagcgaca catagcggct gagagccgtg
     8221 accagcagaa acaccagcag cgccgcgaac aagggcacag gcgccagaac cagatatact
     8281 ccagcggcag tggcaacgcc ttttccgcct ttgaaattca gccaaaccgg gaacatgtgg
     8341 ccaaggatca ccgccaaagc tggcagcaac aacatattgc gctctgtggg tagagctccc
     8401 gcgaaattgc tcaccaggcc gggaaccacg ttcagaagca gccaggcagc cgcgaagcct
     8461 ttaaacaggt ccaaagccag aaccagaatg cctgtcttta ccccatattg gcgcagggcg
     8521 ttggtggcgc cgatattgcc gctgccaccg gagcgtatgt cctttttgtg aaagaacttt
     8581 cctgccagcc agccaaaggg tatgctgcca atcaaatagg ccagcaggca aaccaaaacc
     8641 caaatcacgg cctgaatcac tgttccgtct ccccgtcttt ccggccgcgg aagatgagct
     8701 tgatgctaac gccttcaaag ctgaacattt cccgtatctg gttgtgcagg aagcgcttgt
     8761 aattctcgct gaccagggag ggctgattgc aaaaaaagac gaaggttggc ggtttcacgg
     8821 ccgcctgggt cagatagtaa atcttcacgt gcctgccggt gctgtgggtg ggtgggcggt
     8881 gttgcacaac ggtttccata aagcggttaa gttcgctggt ggagatacgt ttttcgcttt
     8941 c
//
LOCUS       DAOOWR010000021         8710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8054_length_8710_cov_3.186713, whole genome shotgun sequence.
ACCESSION   DAOOWR010000021 DAOOWR010000000
VERSION     DAOOWR010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_8710_cov_3.186713"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLB85_01165"
     CDS             <1..647
                     /locus_tag="PLB85_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71966.1"
                     /translation="NSLRQIKFAQFRAQVPALLLVAVAFALKAIFPNTGNWLYTIALA
                     LAILGIVVYVFIGLCFRYRQRIPLPPETALLSPIEGRIEHIRGSGDITLLAVRKNILD
                     SVELRSPHSDCQLENGELWLATEAGKINFRFNFNHIQWFAEPNLSAGNIIGIVSGSGS
                     CSVIFPGKPGFSVQPGDAVKAAEPLISLFNTPGPAQPEPAPEKVSTPAEEGGEI"
     gene            650..1507
                     /locus_tag="PLB85_01170"
     CDS             650..1507
                     /locus_tag="PLB85_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71967.1"
                     /translation="MRRKFIPLLIILALLLGALGCALLGRPSLQSDTVEESVINEASG
                     LASSIRTPGLLYTHNDSGGEASVYVLNKRALMPARIDLKGISNRDWEDIATGVDPRDG
                     QPYVFVGDIGDNNAKYPSAFIYRFAEPEILDTLITVAKIARIEFTYEDGPRDAEALFA
                     DPRSGDLYVISKREENCGIYRLAYPQSYDKANVARRVGTLPYSWVTAADISPSGNRIL
                     VKTYSKIFCYKRGKKQSVAESLAGKPKEMPYKLEEQGEAIAFDEHGKGYFTISERLGD
                     TPVELYYYK"
     gene            complement(1871..2308)
                     /gene="dtd"
                     /locus_tag="PLB85_01175"
     CDS             complement(1871..2308)
                     /gene="dtd"
                     /locus_tag="PLB85_01175"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU71968.1"
                     /translation="MRVLIQRVLSARVEVEGKVCGEIGPGMLLFVGFGRDDTESLLPS
                     AVKKIREMRMFSDANGKLNLSVEDTKGSLLAVSQFTLYANCSRGRRPDFTPAAPPDLA
                     ERLYDRFVEMLRETGIPVQTGIFAADMKVSLVNDGPVSFILDF"
     gene            complement(2308..2919)
                     /locus_tag="PLB85_01180"
     CDS             complement(2308..2919)
                     /locus_tag="PLB85_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71969.1"
                     /translation="MIKRVLHFIPNLLLLLALSSCLVHTSLVYFDKDTEGVPNLVSNP
                     GFSAYSLDPRQALLGWSVDMQGDSDAPKNLVFIDGKEAIQGNSSLRVDASPYTVVITS
                     DAFRVSRYGGYYTRLWAKSSTALGPQINLRFITFKSNGKIDRQFRTKLKSTDTWEKKS
                     ISAGFLRPGVAFGRLQITIPPFTEGSVWLDDSGCWEVHRFRID"
     gene            complement(2916..4133)
                     /locus_tag="PLB85_01185"
     CDS             complement(2916..4133)
                     /locus_tag="PLB85_01185"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU71970.1"
                     /translation="MIRRKKFDFTLAGFLVLLIFIGCVSIFTASTTTIGEYTTTQSYW
                     WKQLIFAGISLGMIWLLIRLPMPILDLIIIPAYVLNLLALIAVLFTPAVNGAHRWFSF
                     LGLNYQPSESAKLLTILVVARAISRDNLTEIKQILYGLGLTLMPVLLILIEPDFGTTL
                     VFFFSLLAMLVAAEVPLVYLLLLISPVVSMLSSLWLPAIFIWIAILVVLLLWARLSWV
                     TITIAGILNGFLALIMPMFWNGLKDYQQSRIVSFFNPMADPLGAGYQIIQAKIAIGSG
                     SILGKGWLMGTQKNMNFLPEHHTDFIFSVIGEEFGFIGAFLLLGVFFFFFWRLITDIG
                     EIKVRERKIASAGIFAFLMFQTFINIGMNIGLVPATGIPLPFVSYGGSNLLFNSLAVG
                     VILKYLNERGLMK"
     gene            complement(4130..6046)
                     /gene="mrdA"
                     /locus_tag="PLB85_01190"
     CDS             complement(4130..6046)
                     /gene="mrdA"
                     /locus_tag="PLB85_01190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU71971.1"
                     /translation="MTRSVQSLVFRVTLGVLFLLLVGALFRLQVLKGDYYRRIAESNF
                     VRIRRVVATRGEIYDSKYRPIVSNIPSHDLYLTSGRIGNTERLAMFLKANFGIKPEEL
                     RKLVFEQRFKTYEEILLADNLPYEMILVLSERMGDFPELTIRSGTTRSYMYPNHFTGY
                     VGRIDEKEYSRYREEDYSLNAYIGKTGLECYYEVLLRGRDGKEIMQVDAQGKSLELFS
                     KNTSIPPQNGLSLILTIDNDLQEYANSVFPPGIKGCVIVTDVKTGGILAYLSKPDYDP
                     NIFMQKISTEVWAGLNTPSKPLLDRIIHASYPPGSVYKPVTGSKGLETGVIDRYTRLA
                     SCGGGLKIGNRFFRCWSSAGHGSLNVVDAHKVSCDVFFYDLINRMDLDAVAAHAKACG
                     VVEKTGIDLPNERNGFYPDKKYYRDKLGVTSGLNGYKANLAIGQGEVLTTPLQMNTFY
                     AAIARGGMWVQPYLMKQTMGSRRITRDQLQALNKHPLPWSASTMKAIRDGLWAVTNAP
                     GGTARAVRVPGATSFGKTGSAQNFMGSVTHAWFCGFIETDKPEIVVTVFMENAGGGGA
                     MAAPVANKIFNYYIGNLEKIRQPAPIPPQFRTREERSGTASEQSEIPDETPGSEPRPA
                     LEEPVETPAGESGT"
     gene            complement(6043..6534)
                     /gene="mreD"
                     /locus_tag="PLB85_01195"
     CDS             complement(6043..6534)
                     /gene="mreD"
                     /locus_tag="PLB85_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOU71972.1"
                     /translation="MIWKHIWTFILGLLCFYAQVLLLPAFELFGVIPNILIAWVVYLV
                     WTREMTPALIVIFIIGLLYDTTQPESFGMHALVLLLIAIILYQFRKPFESESVLARML
                     SLVLANVIFQVVGWLVLGVVYGFGGELVILSLIGLGYNLAISFVVFWLMQLVARLRIV
                     LVE"
     gene            complement(6584..7336)
                     /gene="mreC"
                     /locus_tag="PLB85_01200"
     CDS             complement(6584..7336)
                     /gene="mreC"
                     /locus_tag="PLB85_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU71973.1"
                     /translation="MKKIFLPLALVLVSILLILGSTEGRVKRASMLGKTVFYPFLHSV
                     KLFRTNSALKGEIARLREQQAATTLENLNLRNALKESQTLQGISFDTGAIAFEVGEVI
                     GYSGQFQQRNLVVNKGSAQGVKVGSPVISARGIVGRVVSVAVTNCVVLPFSNPRFQIP
                     VMDKSTSVQGVLQSDLTGKTRMNMIKLGSEISAGDTIVTSNLSTLYPKGYPVGTVARI
                     SDSQDYLFISAELEPFTQVENLEHVFILQGRK"
     gene            complement(7418..8455)
                     /locus_tag="PLB85_01205"
     CDS             complement(7418..8455)
                     /locus_tag="PLB85_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU71974.1"
                     /translation="MFFEWIFGMFSSDLAIDLGTANTLVYARDKGVVLSEPSVVAVSN
                     DSKKIIAIGQQAKVMLGKNPDEVRVIKPMKDGVIADFQVTELMLRDLILRAQRKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGAREVYLVSEPVAAAIGADLPIEEAFGNMVM
                     DIGGGTSEIAVISLSHIVVHNSIRVGGDKMDNDIISYLRKKNNLHVGLQTAEKIKMTI
                     GSAYPLKEELTMDVRGRDIVSGFPVTIKISSEEVREALSETIATMVDAIKRLFERTAP
                     ELSADIAERGIFLTGGGAMLKGLDEKISKTVDLPVYVVPDALECVVKGAGKVLDELDR
                     YRDVLIKRIED"
BASE COUNT         2019 a         2451 c         2380 g         1860 t
ORIGIN      
        1 cgaactcgct gcggcagata aagttcgccc agttccgtgc ccaagtgccg gccctgctct
       61 tggtggccgt cgccttcgcc ctgaaagcca tctttcccaa caccggaaac tggctctaca
      121 ccatcgccct ggctttggct atcctgggca ttgtggtcta tgtcttcatc ggcctctgtt
      181 tccgctatcg ccagcgcatt cccctgcctc cggaaaccgc gctgctttcc cccattgaag
      241 gcaggatcga gcatatccgg ggcagcggag acattacact gctcgcagtg aggaaaaaca
      301 tccttgacag tgtggaactg cgcagcccgc attcagactg ccaactggag aatggggaac
      361 tgtggctggc caccgaggcc gggaagatca atttccgctt caatttcaac cacatccagt
      421 ggttcgcgga accgaacctc agcgccggca atatcattgg cattgtcagc ggcagcggtt
      481 cctgttcggt gatcttcccc ggcaaaccag gtttcagcgt ccagccaggc gatgccgtga
      541 aagccgcgga gcctttgatc agcctgttca acactcccgg cccggctcaa ccagaacccg
      601 ctcccgaaaa ggtttccacc cctgcagaag aaggaggcga gatataacta tgcgccgcaa
      661 gtttattcct ttgcttatca ttttagccct gctgctcggc gcccttggct gcgcccttct
      721 gggcaggccc tcgctgcaaa gcgacacagt cgaagagagc gtcatcaacg aagcatctgg
      781 cctggcttcc agcatccgga cacccggcct gctctacacc cacaacgatt ccggcggcga
      841 agcctcggtc tatgttttga acaaacgcgc cctcatgccc gcccggatcg acctgaaagg
      901 catcagcaac cgcgactggg aagacatcgc caccggagtg gacccccgcg acggccaacc
      961 ctatgtgttt gtgggcgata tcggtgacaa caacgccaaa tacccctccg ccttcattta
     1021 ccgctttgcc gaaccggaaa tcctggacac cctgatcacg gtggcaaaga tagcccgcat
     1081 cgaattcacc tatgaagacg gcccccgcga cgccgaagcc cttttcgccg atccccgcag
     1141 cggcgacctc tatgtgatca gcaaacgcga ggaaaactgt ggaatctacc gcctggccta
     1201 tccccaaagc tacgacaagg caaatgtcgc ccgccgggtt ggcaccctgc cttactcctg
     1261 ggtcaccgcg gcagatatat ccccctccgg aaaccgcatc ctggtgaaaa cctattccaa
     1321 aatcttttgc tacaagcgag gcaaaaagca gtccgtggcg gaaagcctgg ccggaaaacc
     1381 caaggaaatg ccctataaac ttgaggaaca gggcgaggcc atcgctttcg acgaacacgg
     1441 aaaaggatat tttaccatca gcgaacgcct gggcgacacg cccgtggagc tttactacta
     1501 caagtgatgc cccgtcggga atagggtttc ggagtggagc gcgcaacgaa agcatgatcg
     1561 ctctgcagag cgtgttcaaa tagctatcaa cccagagcag atgaccccac agaaaaacga
     1621 agcgatgtag gctggccgtt aaggcccttt gttatgttcc tgatgcgcct cccgcacgat
     1681 gcccgcattt gatgcgagta ttatgcgcgg ggcgtgggag aggaatggtt gcgggcgtta
     1741 agggtgtgac agacgggact cagcagtggg tgcacatccc tcttttaaat caagtggttg
     1801 cgggtgctca agcagcaagc agcacggagg actttatcca ctctaactgc acaagagcct
     1861 aaaaaactga tcagaaatcg aggatgaaac tcaccggacc atcgttgacc agcgagacct
     1921 tcatgtccgc ggcgaaaatg ccggtctgaa cagggatacc ggtttcgcgg agcatttcca
     1981 caaagcggtc atagagcctt tcagctagat cgggtggggc agcgggagtg aaatccggtc
     2041 tgcgtccgcg gctgcaattg gcatagagcg taaattgcga aactgccagc agagagccct
     2101 tggtgtcttc caccgagagg ttcagcttgc cgttcgcgtc gctgaacatg cgcatttccc
     2161 tgatcttttt cacagcggat ggcagcaggg attccgtgtc gtcgcggcca aaacccacaa
     2221 agagcaacat tcccggaccg atttcgccgc agactttgcc ttccacttca acccgggcgg
     2281 aaagcactct ctgaatcaga acgcgcatca gtcgatacgg aaacggtgga cttcccagca
     2341 gccggagtca tccagccaga cggaaccttc cgtgaaaggc ggaatcgtga tctgcaggcg
     2401 gccgaaagcg actccaggac gcagaaagcc ggcgctgatg gatttctttt cccacgtgtc
     2461 ggtggatttg agtttggttc tgaactggcg gtcgatcttg ccgttgctct tgaaggtgat
     2521 gaatcgcaga ttgatttggg ggcccaaggc ggttgacgat ttggcccaga gcctggtgta
     2581 ataaccgccg tagcggctta cgcggaaggc atcggaggtg ataaccaccg tatagggcga
     2641 ggcgtccacc cgcagggatg aatttccctg gatggcttcc ttgccgtcaa tgaacaccag
     2701 attcttgggt gcatcgctat ccccctgcat gtcaacgctc cagcccagca gggcttgccg
     2761 ggggtccaga gaataggcgc tgaagcctgg attggatacc aggtttggca caccctcggt
     2821 atccttgtcg aaatagacca ggctggtgtg caccaggcag gatgacagcg caaggagcag
     2881 caacagattc gggataaagt gcagaacgcg ttttatcatt tcattaaacc cctttcgtta
     2941 agatatttca ggatcacgcc cacagccagg gaattgaaga gcagattaga cccgccgtag
     3001 ctgacaaaag gcaggggaat gccggtggcc ggaacgaggc cgatgttcat gccgatattg
     3061 atgaaggttt gaaacatgag aaaggcgaaa atgcccgcgg aggctatttt gcgctcgcgg
     3121 accttgattt cgccgatatc cgttatgagg cgccagaaga aaaagaagaa cacccccagc
     3181 agtaggaagg cgccgataaa gccaaattcc tcgccgatca cgctgaagat gaagtctgtg
     3241 tggtgctccg gcaaaaagtt catgttcttc tgggtaccca tcagccaacc cttgcctaag
     3301 atggagccgc tgccgatagc tattttggcc tggatgattt ggtatccggc gccgagcgga
     3361 tcggccatcg gattaaaaaa tgagacgatg cggctctgct ggtagtcttt aagcccgttc
     3421 cagaacatcg gcatgatcag cgctaagaag ccattgagga tgccggcaat ggtgatggta
     3481 acccaggaaa ggcgggccca gagcagtaaa acgacaagga tggcgatcca gatgaagatc
     3541 gccggcaacc atagcgaaga aagcatgctc accaccgggc tgatcagcag gagcagatac
     3601 accagcggca cctcggctgc aacgagcatc gccagcaggc tgaaaaagaa gaccagggtg
     3661 gtgccgaaat ccggctcgat caggattagg agcaccggca tcaaagtcag gccaagaccg
     3721 taaagtatct gcttgatctc ggtaaggtta tcgcgagaaa tcgccctcgc caccaccagg
     3781 atggtgagca gtttggcgct ttccgagggc tggtaattca gacccagaaa gctgaaccag
     3841 cggtgggcgc cgttcacagc cggagtgaaa agcacggcga tgagggccag gaggttcagc
     3901 acgtaggcgg gaatgatgat cagatccagg atcggcatgg gcaaccggat cagcagccag
     3961 atcatgccga gcgagattcc agcgaaaata agctgcttcc accaatagct ttgggtggtg
     4021 gtgtattcgc caattgtggt ggtggaggca gtgaatatgg aaacacagcc aatgaagatc
     4081 agcagaacca aaaagccggc cagggtaaaa tcgaacttct tgcgcctgat catgtgccgc
     4141 tctccccggc tggggtttca accggttctt ccagagcagg ccttggttcg gatcctggag
     4201 tttcgtccgg tatctcagac tgctcggaag ccgtgccgga gcgttcttcc cgggttcgga
     4261 actgtggtgg aataggtgcc ggctgcctga ttttttccaa gttgccgatg taatagttga
     4321 agatcttgtt cgccaccggg gcagccatag ctccgccgcc accggcgttt tccatgaata
     4381 ccgtcaccac aatctccggt ttgtcggttt ctatgaatcc gcaaaaccaa gcatgggtca
     4441 cgctgcccat gaagttttgg gcggaaccgg tcttgccaaa actggtggcg ccgggcacac
     4501 ggaccgctct ggctgtgcct cctggggcgt tggtaacggc ccaaagcccg tctcggatgg
     4561 ccttcatcgt gctggcagac cacggaaggg ggtgtttgtt cagggcctgt aactggtctc
     4621 gggtgatgcg gcggctgccc atcgtctgct tcatcagata gggttgcacc cacattccgc
     4681 cgcgggcgat ggcggcgtag aaagtattca tctgcaaagg ggtggtgagc acttcaccct
     4741 gtccgatggc caggttagcc ttgtagccgt tgagcccgga agttacgcca agcttgtcgc
     4801 ggtagtattt cttgtcggga tagaagccgt tgcgctcgtt tggcaggtct atccccgttt
     4861 tttccacaac accgcaggct ttggcgtggg cggcgactgc atcgaggtcc attctgttga
     4921 tcaaatcgta gaaaaagacg tcgcaggaca ctttgtgggc gtccacaacg ttcagggaac
     4981 cgtgccctgc tgagctccag caacggaaaa agcggtttcc gatcttgagt ccgccgccac
     5041 aagaggccag cctggtatag cggtcgatca cgccagtttc gaggcctttg ctgccggtta
     5101 ccggcttgta aaccgatccg ggagggtagg aagcatggat gatcctgtcc agcaggggtt
     5161 tggaaggtgt gttcaatccc gcccagacct cggtgctgat cttctgcatg aagatattgg
     5221 gatcgtaatc cggcttgctc agataggcca gtatgcctcc ggttttcaca tccgtaacaa
     5281 ttacacagcc tttgatcccg ggcgggaaaa ccgaattggc gtattcctgc aggtcgttgt
     5341 cgattgtcag gatcaggctg agcccgttct gtggcggtat cgaggtgttt ttgctgaaca
     5401 gctccagact cttgccctgg gcatccacct gcatgatctc tttgccgtcc cggccccgca
     5461 gcaatacttc gtaatagcat tccaaaccgg ttttgccgat gtaggcattc agtgaatagt
     5521 cttcctcgcg gtagcggctg tattcttttt catctatcct gcccacatag ccggtgaaat
     5581 ggtttggata catgtagcta cgggtggttc cactgcgaat tgtgagttca gggaaatctc
     5641 ccatccgctc tgacaggacc aggatcatct catagggaag gttatccgcc agcaggattt
     5701 cttcataggt cttgaaccgc tgctcgaaaa ccagcttgcg caactcttcc ggcttgatcc
     5761 cgaaattggc tttcaagaac atagccagcc gctccgtgtt gccgatcctt ccactggtga
     5821 gataaaggtc gtgggaaggg atgttgctca cgataggccg atacttgctg tcgtaaatct
     5881 cgccgcgggt agcgacaacc cgccggatgc gaacaaagtt gctttccgcg atgcgcctgt
     5941 aataatcgcc cttcagcacc tgcaaccgga acagagcccc gaccagcagc agaaataata
     6001 cccccagggt aacacggaaa accaagcttt gaacgcttcg ggtcattcca ccaacacaat
     6061 ccgcagccgc gccaccaact gcattagcca gaaaaccacg aaacttatgg ccagattgta
     6121 gcccaaaccg atcagcgaaa ggatcacaag ctcgcctcca aagccataca ccactccgag
     6181 aaccagccag cccaccactt ggaaaatcac gtttgccaaa accagagaaa gcatccgggc
     6241 caacacgctt tcgctttcaa agggtttgcg gaactggtag aggatgatgg caatgagcaa
     6301 caaaaccagc gcgtgcatgc caaaagattc tggctgagtt gtgtcgtaga gcaggccgat
     6361 gatgaaaatc acgatcaggg caggggtcat ttcacgcgtc caaaccaaat agaccaccca
     6421 ggcgatgagg atgtttggaa tcacgccgaa cagctcaaaa gccggaagca gtaacacctg
     6481 cgcgtagaaa cacagcaagc ccagaatgaa ggtccagata tgcttccaga tcatcccagc
     6541 cctctctgga gcgtcagttc aattccaaca gcagtggcca ggctcatttt ctgccctgca
     6601 gtatgaaaac gtgttccagg ttttccactt gggtgaaagg ctccagttca gcgctgatga
     6661 acaggtagtc ctgggaatcg ctgatacgag ccactgtgcc aaccggatag cccttgggat
     6721 acaatgtgga aaggtttgag gtcacgatcg tgtcgccggc gctgatctcc gagcccagct
     6781 tgatcatgtt catgcgtgtt ttgccagtca aatcggattg cagcacgccc tgaacgctgg
     6841 tggacttgtc catcacggga atctggaaac gcggattgct gaagggcaga accacgcagt
     6901 tcgtgacagc gacagaaacc acccggccca cgatcccgcg cgctgaaatc acgggactgc
     6961 cgaccttcac tccctgcgcc gaacccttgt tcacaacaag gttgcgttgt tgaaactgcc
     7021 ctgaatagcc gatcacttcg cccacttcga aagcaatcgc ccctgtgtcg aaactgattc
     7081 cctgcagggt ctggctttcc ttgagcgcgt tgcgcaggtt caggttttcc agggtggtag
     7141 cggcttgctg ttcccgcagg cgggcgattt cacctttgag ggcggaatta gtgcggaata
     7201 gtttcaccga atgcaaaaag ggataaaaga ccgttttacc cagcatagaa gcgcgtttca
     7261 cacggccttc cgtgctgccc aggattagca ggattgacac cagcaccaag gccaggggca
     7321 gaaatatttt cttcacgggc ttatcctcgt aaggtggcta aggcgtcccc acagacgggg
     7381 cgccgatctt atggatgttg tttacagtca gcgcgattca gtcttcgatg cgttttatca
     7441 gcacgtcgcg gtagcggtcc agttcgtcca aaaccttgcc ggcgcctttc accacacact
     7501 ccagggcgtc cggcaccaca tagacaggca agtccacggt tttggaaatc ttttcgtcca
     7561 gacctttcag catggccccg ccgccggtga ggaagatgcc gcgttctgca atatcggctg
     7621 aaagttccgg cgctgtgcgt tcaaacagtc tctttatggc gtctaccatg gtcgcaattg
     7681 tctctgaaag cgcttcccgc acctcttctg agctgatctt gatggtcacc gggaaaccgg
     7741 aaacgatgtc gcgtccgcgc acgtccatgg tgagttcttc tttcaacgga taggcgctgc
     7801 cgattgtcat cttgatcttt tctgcggttt gcaggccgac gtggagattg tttttcttgc
     7861 gcagatagct gatgatgtcg ttgtccatct tgtcgccgcc cacgcggatg gaattgtgca
     7921 caacgatgtg ggagagcgag ataacggcga tttcgctggt gccgccgcca atgtccatca
     7981 ccatgttgcc aaaggcttct tcgatgggca ggtccgcccc gatggccgcg gccacaggct
     8041 cggaaaccag ataaacttcc ctggccccgg catgcaaagc actgtcccgc acggcccgtt
     8101 tttccacttc cgtgattccc gaaggcacgc agacgatgac ccgcggacgc accagcaggc
     8161 gctttctttg agcccgcagg atcaggtcgc gcagcatcag ctccgtcacc tggaaatccg
     8221 ctatcacgcc gtctttcatg ggtttgataa cccgcacttc atcagggttt ttgcccagca
     8281 tcaccttggc ctgctggccg atggcgatga tctttttact gtcgttggaa accgccacca
     8341 ccgagggttc ggacaggaca actcccttat cccgggcata tacaagggta tttgccgtac
     8401 ccagatcaat ggcaagatcg ctggagaaca tcccgaaaat ccattcgaag aacatagatg
     8461 cccactcctt tccttcatgc ggcgctattg ataggccgtc ttagcaaata cccacctctt
     8521 ttacaagaga agagaagaaa gcgctcgtct cttgcacaaa gcccccgctt atgatagcaa
     8581 tgcagcacga tgcagaaaag ttgtaccata atactggccg caggaaaagg caccagatta
     8641 aaatctgaaa caccgaaggt tctgcacgag attctcggcc tgccgctgat ctattatccc
     8701 ctcaaccttg
//
LOCUS       DAOOWR010000022         8630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8140_length_8630_cov_3.665190, whole genome shotgun sequence.
ACCESSION   DAOOWR010000022 DAOOWR010000000
VERSION     DAOOWR010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8140_length_8630_cov_3.665190"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLB85_01210"
     CDS             complement(<1..268)
                     /locus_tag="PLB85_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71975.1"
                     /translation="MRTLLIVAVCLVCVAATDGLYGNPALEAAETAEQLFEQALSLYQ
                     NDGAEQDPALAVKLFRLAKDGGVDEAQYYLGLCYLQGRGVGKDEK"
     gene            429..653
                     /gene="rpsP"
                     /locus_tag="PLB85_01215"
                     /pseudo
     CDS             429..653
                     /gene="rpsP"
                     /locus_tag="PLB85_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            730..960
                     /locus_tag="PLB85_01220"
     CDS             730..960
                     /locus_tag="PLB85_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU71976.1"
                     /translation="MKELIEFMVKALVDDPSEVNITEIQGDKITLYELRVSKTDIGKV
                     IGKRGRTAGAMRTIINAVSTKQGKRAELEIIE"
     gene            961..1470
                     /locus_tag="PLB85_01225"
     CDS             961..1470
                     /locus_tag="PLB85_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71977.1"
                     /translation="MKVSDLTAVGRLGGMDADGFFQVMVKPHYRKILTETEEIFLIFN
                     SDRVFFVTISDRDISDRKLRLRFAEDGIDRERKLHREVMLALPPEEEESDEPDELLGY
                     TMVFQDREVGKVTDYFHNNAQYVLVVTAPDGTEILIPQVDHFVGEVIPDPGVVVLINA
                     ESLLATSTT"
     gene            1467..2150
                     /gene="trmD"
                     /locus_tag="PLB85_01230"
     CDS             1467..2150
                     /gene="trmD"
                     /locus_tag="PLB85_01230"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU71978.1"
                     /translation="MKLNVLTLFPEAFASVLESSMIARARAMKALEVEFTDFRTYSGN
                     KHNQVDDYAFGGFAGMVLRAEPVYQAITELVKESWAPVVYFTPQGRKLDQAVLEYYAG
                     LSRVILLCGHYKETDQRLRDLAVCDEISIGDYVLSGGEIPALAFIDGVARLLPGVLGD
                     ISSAASDSFSAGSDELGFSCYTRPVNWMGQSVPPVLREGNHAKIQAWANESAKRFTRV
                     RRPDLKRGK"
     gene            complement(2228..2512)
                     /locus_tag="PLB85_01235"
     CDS             complement(2228..2512)
                     /locus_tag="PLB85_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71979.1"
                     /translation="MSKGYLVSQLPRCRGNDERTLFFLRCYLVYQHIDRIAGANPGQG
                     LAALRIIGQSLILPEVSDVVEQFLLAEVGMNNIISLNAVGFSRPGNVMDV"
     gene            2715..3068
                     /gene="rplS"
                     /locus_tag="PLB85_01240"
     CDS             2715..3068
                     /gene="rplS"
                     /locus_tag="PLB85_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOU71980.1"
                     /translation="MDILHTVGRDQIRTDFPEYRPGDTVKVHYKIKEGAKERIQVFQG
                     IVIQKRGSGISKSFTVRKISNGVAVERIFPQNSPNIDKLEIVRYGQVRRAKLFYLRHA
                     KGKAGRIKERRRFTA"
     gene            3157..3624
                     /locus_tag="PLB85_01245"
     CDS             3157..3624
                     /locus_tag="PLB85_01245"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOU71981.1"
                     /translation="MAEQQVGYVDWEDWGLELVMERGKLRKLLFVEKGKAAEALEPEM
                     AKACQQLLAYLNGNGKAFKLELEPIGTNFQLEVWNALMEIPYGETRSYKQLAERIGRP
                     RACRAVGQAANKNPLPIVIPCHRLVGSNGSLTGYAGGIEIKRRLLELERRHLE"
     gene            3633..4031
                     /locus_tag="PLB85_01250"
     CDS             3633..4031
                     /locus_tag="PLB85_01250"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOU71982.1"
                     /translation="MGIGTDIVKVERVRRLLERYPNFVERVFCAGEITHCQGKANPEQ
                     SYATRFAAKEAVMKALGTGWDGRVNWRDIEVFTDKNGKPDILLRGGALERLTALAADR
                     VHLSLTHEKEYALAFVVLERQKLDSLRVSG"
     gene            4056..4694
                     /locus_tag="PLB85_01255"
     CDS             4056..4694
                     /locus_tag="PLB85_01255"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOU71983.1"
                     /translation="MRCFVFFGIQGSGKGTQADLLGDSLKFQHVSIGDLFREQISLKT
                     KLGLQVSEIISRGELVPDELVFDIIDQSLHPDRKGIIFDGFPRTLAQAEFLVKHFKVR
                     QVFFLELSEAKAIARISSRRICRDCGQNYNLVSDPPSEVNVCDACGGKLIIRNDDKPE
                     AISRRLAEFYEQTLALKDFFAERGLLSEVDAGQEIEAVAKDIREIVASLPAE"
     gene            4697..6385
                     /locus_tag="PLB85_01260"
     CDS             4697..6385
                     /locus_tag="PLB85_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOU71984.1"
                     /translation="MRKILKLILSPVEAAAYLLAVWSLLVLFLEPLISYYSNYQLVQT
                     ATAWVNLLLLALAILHRLLLREGRAIPIVVIFDTVMLVVGLLLMNFNARFVIFFLLVR
                     QTYFILDFILFRFSRGRIYKWLADNPPVTMMLSFILVILIGTILLMLPVASTQKRVTP
                     FVNALFTSTSATCVTGLIVYDTATYFSRFGQVVILLLIQIGGLGIMTVSTAFALLMGR
                     NIDLKLKNVMSQVVGGTNRINLAQLLKNILLVTGIIELTGAALLFSRFYHHHSLARAL
                     YLSMFHSVSAFCNAGFALFSDNLVGFRDSAVVNIVIPLLIIFGGLGFSVIIDLYRYFF
                     KRDTVRKLTLHSKIVLVTTAFLIVGGMISFFVMEYYGTMKGFGIHQRLLSSFFQSVTT
                     RTAGFNTLDFGAMGKATLLVSMFLMFVGASPGSTGGGIKTTTFSILGLTMVSLLRGRR
                     DISVFKRRIPSSNFREAAGLVFLSASLVFFIVLILLLVEPSPFEKIMFEVISAFGTVG
                     LSTGITPSLSFLGKILITLLMYVGRIGPLTLIYAFAVRNRKTNINYAEENIAIG"
     gene            6396..7106
                     /locus_tag="PLB85_01265"
     CDS             6396..7106
                     /locus_tag="PLB85_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOU71985.1"
                     /translation="MARYAVIGLGRFGMTVATILAENGMEVLAIDKKQELVDQISSKV
                     AQAVCMDSTDENSLRDMNLNEVDAVIMAIGSNIQESILSCAILKKIGVGIIYAKIENH
                     LHGRILELIGVKRTILPEETVGSQLANTLISKNILEYYNLTSGHVVLELVAPLEFVGK
                     SLQELALPTGRGINVIAIKYNYLTVTDEGKNKVEQRLNDMPGANDVINEGDVMMLMGR
                     KNAIDSLIRDVANKRDLL"
     gene            7103..7852
                     /locus_tag="PLB85_01270"
     CDS             7103..7852
                     /locus_tag="PLB85_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71986.1"
                     /translation="MKHSISKRYHLAISILLGLILLETLLLWIIISRKTDLATMVNDL
                     QTTLIVSVLAIFVCGVVIYNLIPVMLRKSLSRTAKIVSEIAHGNYEVNIDGELAAHAG
                     DKDIVPLLEGLKIMLRNIHGFDQAKENKIYEHDQRIKQMINLLPQGVLIALNNGDISY
                     CNDALRRRYPMFNESNNIQDLKLKSEFDQRIFQKIFDSLRYGDNLYDNRIPDNDYQCL
                     ATLNGAIVRNARGDSIGGVYTLVFTEHAKQD"
     gene            7836..>8630
                     /locus_tag="PLB85_01275"
     CDS             7836..>8630
                     /locus_tag="PLB85_01275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU71987.1"
                     /translation="MQNRIKVVFDKDGTDRLDRYLVDLRIQELYSRSFIEKLIEEDRV
                     LVNKVPVKKSYLLTKGDEVDVSLPEFKPAEVVPQNIPLDVVYEDDDIAVINKPAGMIV
                     HPGYGASDQTLVNAIVFRWREDLSSGREANRPGIVHRLDRGTSGLMVIAKNDPVQSAL
                     NDMFAQRQVKKTYLAITCGIPDPAEDSIEDNIGRSISNPRKMCVTPEGRWSLTNYKVI
                     KYFHCFSLVKVSLETGRMHQIRVHFAHRNLPLLGDLLYNTRRQVHAI"
BASE COUNT         2192 a         2150 c         2270 g         2018 t
ORIGIN      
        1 gcttttcatc tttgccgaca ccccggccct ggaggtaaca aagccccaaa taatactgtg
       61 cctcgtccac accaccgtct ttggccagac ggaacaactt caccgccaga gctggatcct
      121 gctccgcccc gtcattctga tacagcgaca atgcctgttc aaaaagctgt tcggctgttt
      181 ctgcggcttc aagtgccgga ttaccgtaaa gtccgtctgt ggctgcaacg cacacaagac
      241 acacagcgac gatcagtaaa gtacgcaatt gacacctccc atgccatggg ttttctttta
      301 ttcaagccgg gcaataatgt caagtaaaaa ttgcgtttcc acagcgagaa atcccttgac
      361 gaaaatagga cttacaaaaa tgttggcgca aaatagactc atcttattga taatagcaag
      421 gagaaataat ggtcaagttg agactgagaa ggatgggagc gaacgaccag cccttttacc
      481 ggattgttgc aattgactcc cgtgtgagcc gcgatggcaa gtacatagag agcattggct
      541 ggtatgaccc caaacccaac ccttccaaaa tccagatcga aaaagatcgc gccctgtatt
      601 ggcttggagt cggcgcgcaa cctagcgaca ccgtgcgttc gctgctgcgc aaggctggca
      661 tcctgcagat ccggcacgag cagaaaatag aaagcagaaa agcagcagaa aacacacaag
      721 aggtaagaaa tgaaggaact gattgagttc atggtcaagg cactggttga cgatccaagc
      781 gaagtcaaca tcacagagat tcaaggcgac aagataactc tttacgagct tcgtgtttcc
      841 aagaccgata ttggcaaggt gatcggcaaa agaggccgca ccgctggcgc catgcgcacc
      901 atcatcaacg ccgtcagcac caagcagggg aagagagccg agctcgaaat catcgagtag
      961 atgaaggttt cggacctgac agccgtcggc cgtttgggcg gaatggacgc ggacggtttc
     1021 ttccaggtga tggtgaaacc gcattaccgc aagattctga ctgaaaccga agaaatattt
     1081 ctgatcttta acagcgatag agtgtttttc gtaactatta gtgatagaga catatccgat
     1141 cgtaagctgc gattgagatt tgctgaggac ggaatagaca gggaacgaaa gcttcaccgc
     1201 gaggtgatgc tggcgctacc tccagaggaa gaagaatccg acgaaccgga tgaacttctg
     1261 ggctacacta tggtttttca ggaccgcgaa gtcggcaagg tgacggacta tttccacaat
     1321 aacgcgcaat atgtactggt ggttaccgct cccgacggga cggagatact catcccgcag
     1381 gttgaccatt ttgtcggcga ggtaattccc gatcccggcg tggtcgtttt aatcaatgcc
     1441 gagagcctgt tggcaaccag caccacatga agctaaacgt cctcaccctt tttccggaag
     1501 cttttgccag cgttctggaa agcagcatga tcgcccgcgc cagagccatg aaagctttgg
     1561 aagtagagtt caccgatttc cgcacctata gcggaaacaa gcacaatcag gtggacgact
     1621 atgcttttgg cggattcgcc ggcatggtgc ttcgcgccga accggtgtat caggcgatca
     1681 ctgaactggt caaggaaagc tgggccccgg ttgtgtattt caccccccag gggcgcaaac
     1741 tcgatcaggc ggtgttggaa tactatgccg gcctgtcacg cgtgatcttg ctttgcggac
     1801 actacaagga aactgaccag cgactgcgtg acctggctgt ttgcgatgaa atctccattg
     1861 gagactacgt cctttcgggc ggcgagattc ctgccctggc tttcatcgac ggcgtggcga
     1921 ggctcttgcc cggtgttttg ggagacatct catccgccgc cagcgattcc ttcagcgcag
     1981 gcagcgatga acttggtttt tcctgttaca ctcggccggt gaattggatg gggcagagcg
     2041 tccctccggt gctgcgtgaa ggcaatcatg ccaagattca agcttgggcc aacgaaagcg
     2101 cgaagcggtt cacccgggtg agacgtcctg atctgaagcg gggcaaatga gcgtccttta
     2161 tattgccctg gcgcatcatc cagtgctgaa caaagccggt gagaccgtga ccacttccat
     2221 cagcaaccta gacatccatg acattgcccg ggcggctgaa acctacggcg ttaaggctta
     2281 ttatattgtt catgccgacc tccgccagca ggaactgctc aacaacatca gaaacttctg
     2341 gcaggatcag ggattgccct ataatccgca gcgcagccag gccctggccc ggattcgccc
     2401 cagcgattcg atcgatatgt tgataaacga gataacaacg caggaagaaa agcgtccgct
     2461 catcgttacc acgacagcgc ggaagctgcg agaccagata tcctttggac atctgaaaac
     2521 tctcacccta aatgacaggc cggttttgct catatttggc accggttatg gattatgcga
     2581 tgaagttcat caagcggcgg accacgtcct cgagccggtt acaggcccgg gggactacaa
     2641 tcatctgtcc gtccgtagtg ccgtgagcgt cgtgttggac cgcctttctt ccgagtacat
     2701 atatgggagg aaccatggat attctgcaca cagttggcag agaccaaatc agaaccgact
     2761 ttcccgagta tcgccccggc gacactgtga aggtccatta taaaatcaaa gaaggcgcga
     2821 aggaacgcat ccaggtgttc cagggaatcg tgatccagaa acgcggatcg ggaatctcca
     2881 aatccttcac cgttagaaag atatcgaacg gcgttgccgt agagcgtatt ttcccgcaga
     2941 attcgccgaa catcgataag cttgaaatag tccgctatgg ccaggtgcgc agggccaaac
     3001 tcttttacct gcgccatgca aaaggcaaag cgggccgcat caaagagcgc agacgtttca
     3061 ctgcctaatt gcgaaacact atccctcaga gccccagact cacttcagag aaaggggctc
     3121 tcgttttttt ggcaacataa acaaccgagg taaggaatgg ccgaacagca agttggctat
     3181 gtggattggg aggactgggg ccttgaactg gttatggagc gaggaaaact ccgcaagctg
     3241 ctttttgtgg agaaaggcaa ggcagcagaa gccctggagc cagaaatggc caaagcctgc
     3301 cagcagttgc tggcctatct gaatggaaat ggcaaggctt tcaagctgga gctcgaaccc
     3361 attggaacta actttcagct ggaagtttgg aacgcgctta tggaaatccc ctacggcgaa
     3421 acccgcagct acaagcaact cgctgagcgg attggacgcc cccgagcctg tagggcggtg
     3481 gggcaggctg caaataagaa tcccctgccc atcgtgattc cctgccaccg gttggtgggc
     3541 tcgaacggtt ctcttaccgg ctatgccgga ggcattgaaa tcaaacggcg cctgctggaa
     3601 ctggaacgca ggcatttgga gtagaattga tagtggggat aggaaccgat atcgtgaaag
     3661 tggagcgtgt gcgcagactg ctggaacggt atccaaactt cgttgaacgt gttttctgcg
     3721 cgggcgaaat cacccattgc cagggaaaag cgaaccctga acaaagttac gccacccgtt
     3781 tcgccgccaa ggaagccgtg atgaaagctc tgggtacggg ctgggacggc agggtaaatt
     3841 ggcgggacat cgaggttttt acagataaaa acggaaaacc ggacatcctg ctcaggggcg
     3901 gggcgctgga acgtttgacc gctttggcgg cagatcgcgt tcacctgtcc ctgactcacg
     3961 aaaaggaata cgcgctggcc tttgtggtgc tggaaaggca aaagcttgac agcttgcgag
     4021 tatctggata atttaccaaa attgaggtgt tttatatgcg atgtttcgtg ttcttcggca
     4081 ttcagggtag cgggaaaggc acacaggcag acctgttagg tgacagcctg aagttccagc
     4141 acgtcagtat tggcgatctc ttccgggagc aaatctccct aaaaaccaag ctcggtttgc
     4201 aggtgagtga aataatcagc cgcggtgaac ttgttcccga cgaacttgta ttcgacatca
     4261 tagaccaatc gctgcatcca gatcgcaagg gaatcatctt tgacggattt ccccgcactt
     4321 tggcgcaggc ggagtttttg gtgaagcact tcaaggtgag gcaagttttc ttccttgaac
     4381 tttccgaagc caaggccatt gccaggattt cctcgcgccg aatctgccgt gattgcggcc
     4441 aaaactacaa tctggtgagc gatccgccaa gcgaggtaaa tgtttgcgat gcctgcggtg
     4501 ggaaactgat catacgcaac gacgataaac cagaggctat ctctcgccgt ttggctgaat
     4561 tttacgaaca gaccctggcc ttgaaggatt tttttgccga gcgtggtctg ctttctgaag
     4621 tggatgctgg tcaggagatc gaagcggtgg ctaaagatat cagagaaata gtggcatcgc
     4681 tgcccgctga gtgagtatgc gcaaaatcct gaagctgata ctgagcccag tggaagcggc
     4741 agcgtattta ctggccgtgt ggagcctttt ggttcttttt ctggaacccc tgatcagcta
     4801 ctattcaaat tatcagttgg tgcaaaccgc cacagcctgg gtcaatctgc tcctgctggc
     4861 tttggctatc cttcaccgtc tgttgctaag ggaaggacgc gccatcccga ttgtggtgat
     4921 cttcgacacg gttatgttgg tggtgggatt gttgctgatg aacttcaacg ccaggttcgt
     4981 cattttcttc ctgcttgtcc ggcaaactta cttcatactg gatttcatcc tcttccggtt
     5041 ctcccggggc agaatctaca aatggctggc agacaacccc ccggtgacca tgatgcttag
     5101 ttttatcttg gtgattctta tcgggaccat attgctgatg ctgcctgtgg catcaacaca
     5161 aaaaagagtg acgcctttcg tgaacgctct attcacctca acatcagcaa cctgcgttac
     5221 aggcttgata gtctatgaca ccgcaaccta tttcagcagg ttcgggcaag tggtgatcct
     5281 gttgctgatc cagatcggag ggttgggtat catgacagtt tcgacggctt ttgccctgct
     5341 aatgggcaga aacatcgatc tgaagctcaa gaacgttatg agccaggtgg tgggaggcac
     5401 caacagaatc aacttggctc agctcctaaa aaacattttg ctagtgaccg ggataataga
     5461 actgacagga gcagcgctgc ttttcagcag gttttatcat caccattcgc tggcacgggc
     5521 gctttatctc tccatgttcc attcagtttc agccttttgc aacgctggtt tcgctctttt
     5581 ttcggataac ctagttgggt ttcgcgacag cgctgtggta aacatcgtta ttcctttgct
     5641 tatcattttt ggaggtttgg ggttctcagt tattatcgat ctttaccgct atttcttcaa
     5701 gcgcgacact gtgcggaagc tgaccctgca cagcaagata gtgctggtaa ccaccgcatt
     5761 tctcattgtg gggggaatga tctccttttt tgtgatggag tattacggta cgatgaaagg
     5821 cttcggaatc catcagcgct tgctgagttc tttcttccag tctgttacca cgcgcacggc
     5881 gggtttcaac acacttgatt tcggtgctat gggtaaagcg accctgttgg ttagcatgtt
     5941 tctgatgttc gtcggcgctt cgccaggctc cacaggcggc gggatcaaga ctacaacttt
     6001 ttccatatta gggctcacga tggtctcact gctgaggggg agaagagata tcagcgtgtt
     6061 caagcggcga atcccttcca gcaatttccg ggaagcagca ggactggtat ttctttcagc
     6121 ctccctggtc tttttcatag ttttgatact gttactggtg gaaccctcac cttttgaaaa
     6181 gattatgttc gaagtgattt cagctttcgg cacagtgggc ctttccaccg gtatcacacc
     6241 ttcactttcc tttctgggaa aaattctgat aactcttctg atgtacgtcg gcaggatcgg
     6301 tccgctgact ttgatctacg ctttcgcagt tagaaacagg aaaaccaata tcaattatgc
     6361 cgaggaaaat atagccatcg gctaagggaa taattatggc acgttacgcg gtaatcggac
     6421 tgggccggtt tggcatgacc gtggccacga tcctggcaga gaacggcatg gaagttcttg
     6481 ccatcgacaa aaaacaggaa ctggtggatc aaattagcag caaggtcgcg caggctgtgt
     6541 gcatggactc cacagatgaa aactctctga gggacatgaa tctgaacgaa gttgacgcag
     6601 tgatcatggc tatcggcagc aacatccagg aatccatcct cagttgcgcg atcctcaaga
     6661 aaatcggcgt tggcatcatc tacgccaaga tagaaaacca tctgcacggc cgtatccttg
     6721 aactgatagg agttaagcgc accatccttc ccgaagagac ggtgggatcg caactggcta
     6781 atacgctgat atcgaaaaac atcctggagt actacaatct aaccagcggc catgtagtgc
     6841 ttgaactggt cgcgcccttg gaatttgtcg gtaagtctct gcaggagttg gctctgccca
     6901 ctggcagggg gataaatgtg attgccatca aatacaatta cctgacagtg actgacgagg
     6961 gcaagaacaa ggtggaacag aggctgaatg acatgcccgg agccaatgat gtaatcaacg
     7021 aaggggatgt gatgatgcta atgggaagaa aaaacgccat cgactccctg atccgggacg
     7081 tcgcgaacaa gagggacctg ctatgaaaca ctctattagc aaacgctatc atcttgccat
     7141 atccatcctg ctgggactga tcctgctgga gacactcttg ctatggatca tcatcagccg
     7201 gaaaacggat ttggccacca tggtcaatga tctgcaaaca accctgattg tttcagtttt
     7261 agctatcttt gtctgcggtg tggttattta caaccttatt ccggtgatgc tcaggaaatc
     7321 cctgagccgt acggccaaaa tcgtcagcga aattgcccac ggtaattacg aggtgaatat
     7381 agatggcgaa cttgcagccc atgccggaga caaggatatt gtgccacttt tggaagggtt
     7441 gaagataatg ttgcggaaca tccacggttt tgaccaggcc aaggaaaaca agatttacga
     7501 gcatgatcag cgcatcaaac aaatgattaa cctattaccc cagggggtcc tgatcgccct
     7561 caataacggc gatatcagct attgcaacga cgccctgcgg cggcgctacc ccatgttcaa
     7621 tgagagcaac aatatccagg acctgaagct gaaaagtgaa tttgaccagc gaatctttca
     7681 aaagatattc gattcccttc gttacggcga taatctctat gacaacagga tacccgataa
     7741 cgattaccaa tgcctggcaa ccctaaacgg cgccatcgtg cgtaacgcta gaggcgattc
     7801 catcggtgga gtttatacac ttgtcttcac ggagcatgca aaacaggatt aaagttgttt
     7861 ttgacaaaga tgggactgac cgtctggaca gatacttggt agatttacgc atccaagagc
     7921 tatacagccg tagcttcatc gagaagctga tagaagaaga ccgtgtgctg gtgaacaagg
     7981 tcccggttaa aaagagttat ctgcttacca agggtgacga ggtggatgta tccctgccgg
     8041 aatttaagcc tgcagaggtt gtgccgcaaa acattcccct ggatgtggtt tatgaggatg
     8101 acgacatagc cgtgatcaac aaacctgccg gtatgatcgt ccatccgggt tacggggcat
     8161 ctgaccagac gctggtgaat gccatcgtat tccgctggcg ggaagacctt tccagcggtc
     8221 gggaagccaa tcgccccggc atcgtgcacc ggcttgaccg aggcacatcc ggtctgatgg
     8281 tcattgccaa gaatgacccg gtccaaagcg cgcttaacga catgttcgcc caacgccagg
     8341 tgaaaaaaac ctatctggct ataacttgcg gaattccgga cccagccgag gacagtattg
     8401 aagacaacat cggccgcagc atcagcaatc cacgcaagat gtgcgtgacc cctgaaggac
     8461 gatggtcgct taccaactac aaggtaatca agtacttcca ttgcttctcg ctggtgaagg
     8521 tatcactaga aacaggaagg atgcatcaga tcagagtgca ttttgcgcat cgcaacctgc
     8581 ctcttctggg agaccttctt tacaacaccc gcaggcaggt tcatgccata
//
LOCUS       DAOOWR010000023         8487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8296_length_8487_cov_3.928487, whole genome shotgun sequence.
ACCESSION   DAOOWR010000023 DAOOWR010000000
VERSION     DAOOWR010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8296_length_8487_cov_3.928487"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="PLB85_01280"
     CDS             <1..2246
                     /locus_tag="PLB85_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU71988.1"
                     /translation="KEQLRLRQSLARKDFDGSIRQMLAYIARLNHVILDPKPTQSWEN
                     IYYKRHIAAGIPSMYGQYREPKLEAMGMIFRLESLVERLVERNIKQLNLDYISGKTLK
                     RIIRILELFEIGLAREGLTSEAFSSALEMLKSGQRIVNLSLDQYLDLFKQIKDSITEL
                     INEYYYRFYEQELENAPVKGVSGKYKRQTARQMFAEDFFRKLLASSFLVQHMDSFIAQ
                     ILASLDAMKRVFGSAELSRVMSYDPDLMFIPLHTSNGKLDNQVLLGSKAFFLKKMCRY
                     DFPIPPGFVITTELHRYGNIVRSHPGIEREMDELLRYNITRLESQTGLRYGDPEAPLL
                     FSVRSGAPMSLPGAMATFLNIGLNDEITLKLSTRPNYGWTAWDCYRRLIQSWGMAYGI
                     DRDEFDAVMISFKRRHKVKLKTQFTPLQMRQMSESYKKVLARHKLELEQDVFLQVKQA
                     IMHVLDSWNTDRARLYRQKLQIADDWGTAVTIQKMVLGNICLDSGTGVLFTHAPWNKA
                     PGITLNGDFTLCSQGEDVVAGLVHTLPISEQERKQSKQKVELSLEKDFPQIYDRLFSL
                     ASHLLDERGYPHQEIEFTFESPARDQLYILQTRNQVITKARAYTVFNVPKRELKLLGN
                     GIGIGKGVINGLIAISRADIDALKDRGQPLILVRPDTVPEDMELLFDCQGLLTSRGGV
                     TSHAAVTAIRLGLVGIVNCRQLKVFEDQNNCRIGETLFKPGDEIAIDAGSGAIYLGHY
                     PVETISR"
     gene            2281..3534
                     /locus_tag="PLB85_01285"
     CDS             2281..3534
                     /locus_tag="PLB85_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOU71989.1"
                     /translation="MNLDLKNKKLLGINGLGRIGKLLLWNQIHLRNFDGIVINFGREV
                     GKKLDDFIQVIESDSTYGSIHKFLFGMVGRRAEIKILDPDMPLISIEGIPVKILRKAR
                     DPKDINWLNEGVRIVVDCTGNFVDPNATTEQGKPCLRGHLAAGALKVVCSAPFKSKTA
                     SVTSSPDAITMIYGINHLNYKPAEHHVISAASCTTTGLAHMIKPLLENEETSNIMTAS
                     MSTIHAATNTQSILDSVPKTGATDLRKSRSMLNNIILSTTGAAKALELVMPKIKSVGF
                     MADSVRIPTSTVSLINLNMTFHTKLNEQGQPLVNRRLINEIYQKAAEGPQKGLLVYSE
                     RQNVSADLIGSLAAATLEAHETHTRTSFIPVPPQTLAALGVPADREVTMPVTHAKICG
                     WYDNELGSYTHCLSLLVKHIQSTMP"
     gene            4443..5099
                     /locus_tag="PLB85_01290"
     CDS             4443..5099
                     /locus_tag="PLB85_01290"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU71990.1"
                     /translation="MKCPVCNATNPDHATFCKSCGKALRSNLRTCPNGHDYDASLPSC
                     PHCPPQRTANLGQAPTARMGDEADRTRLDTAKPRLGAGISGPGAPLSDKTVILERPEA
                     APGAVPSAEGKPRPQVNRMLTGWLVTFDVDPNGLDFKVYQGRHVIGSSASCDIQLKLP
                     GVSAEHAVLLSRNGKFIIEDNLSSNGTFVNGEMIEDKVYLNENDIIRIATIDLKLKTV
                     "
     gene            5108..6655
                     /locus_tag="PLB85_01295"
     CDS             5108..6655
                     /locus_tag="PLB85_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU71991.1"
                     /translation="MRRLMILLLLCAPLWAQNISLNHLKTNTDNFPKIVNSSVLALDN
                     AGEPLKNLNAGNFRISLGGAAVDTLLNVTTFESSGQGLSILVCVDVSGSMKGKPLDSV
                     KNALLSFIGKKRANDEIAILAFSEKEELVCDFSTDTEFLRESIGKLKILPGDTSLYYC
                     IDKGLDRLGGPTARNEGRFMILFSDGKEDNPATAYTIDGVIEKARAKQIPIFTAGYTR
                     QEKQYLQILDRISQQTGGSFYDAPDAQSLSDHFGKVSRQILGSYVLTYPVFGLAGDGL
                     EKDLSITLTQGTYKAEFPEPVKVRVPANREAISGPEEIDAKGGGKLSLGWIIGIIAVL
                     LAAAAGIFFWLKDKEQKRLAEQRRQQEEEERQRREQQARYEEEQRRLEEQTRREAEIR
                     AQDQTPRSREHTVILSPGTGTGESRGEQGLSLEVMMGSEQGRTFNLGAEGATLGRAQG
                     NSIVFNDSTVSSRHARIYWSDGHFYIEDLGSLNGVYVNGRKVSLCRIEQGSTFKLGSN
                     EGVFRLY"
     gene            6664..7713
                     /locus_tag="PLB85_01300"
     CDS             6664..7713
                     /locus_tag="PLB85_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOU71992.1"
                     /translation="MSRKTAERLVISNLTDVGMVRESNQDYYGKYNGGFGDLILVCDG
                     MGGYRGGEVASQVAVETISAHFQGLGAEYDEINELHRAFSLAQQNIARHASENPELAE
                     MGTTAVALLIRGEQYWIAWIGDSRIYRRRGGSTIQLSKDHSWVQGMVDQGLIKAEDAQ
                     DHPKKNIILRSLGPEPHASEAAGPFPIFRGDIFMLCSDGLSDYFSLAEVDRYLALEPQ
                     QACRAMVDEAKRRGGKDNITIQVLNVVRGKAFRPTPGGTNLLTLGLALLALVFLICGG
                     INFQRLAREEKRRKEAEIAAVSDSIAIIAPDTTGTVNETGAPETGNPGKDSGSQGKNT
                     FPPSKKVNKPNQGGH"
     gene            7858..8337
                     /locus_tag="PLB85_01305"
     CDS             7858..8337
                     /locus_tag="PLB85_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71993.1"
                     /translation="MSNSRFYFSFRWWHIPLILLVLGLGFLAWASTWDFDPEVKPHIF
                     PNPSLDVKNNAWVGYWVQPFEIRFIDRDNEIPLILESTEPRFVKARQTTGIPLRIKAG
                     GIENLRLKSLLRIRVQSIVTAGRWGLSKTMRVDREMSLPELLDQTIKLSTDVLPQLP"
BASE COUNT         1958 a         2735 c         2297 g         1497 t
ORIGIN      
        1 aaaaggagca gcttcgcctg cgccagagcc tggcccgcaa ggatttcgac ggtagcatcc
       61 gccagatgct ggcttacatc gcccgcctca accacgtgat attggacccc aaacccaccc
      121 agtcctggga aaacatctac tacaagcgcc acatcgcggc cggtatcccc tccatgtacg
      181 gccagtaccg cgagccgaaa ctcgaggcca tgggcatgat cttccgcctg gaaagcctgg
      241 tggaaaggct cgtggagcgg aacatcaaac agcttaacct ggattacatc tccggcaaaa
      301 ccctcaagcg catcatccgc atcctcgagc tcttcgaaat cggcctggcc cgcgaaggcc
      361 tcaccagcga ggctttttcc agcgccctcg agatgctcaa atccggccag aggatcgtga
      421 acctctccct ggaccagtat ctggacctct tcaaacagat caaggacagc atcacagagc
      481 tcatcaacga gtattactac cgcttttacg agcaggaact ggaaaacgcc cccgtgaagg
      541 gagtttccgg caaatacaag cgccagaccg cccggcagat gttcgccgag gactttttcc
      601 gcaagctgct ggcctcttcc ttcctggtgc agcacatgga cagtttcatc gcccagattc
      661 tggcctctct ggacgccatg aaacgggttt tcggctccgc cgagctcagc cgcgtgatga
      721 gctatgaccc ggacctgatg ttcatccccc tccacaccag caacgggaag ctggacaacc
      781 aggtgttgct gggttccaaa gccttcttcc tcaagaagat gtgccgctat gatttcccca
      841 tcccgcccgg tttcgtgatc accaccgagc tgcaccgcta cggaaatatc gtgcgctcgc
      901 atcccggcat cgaacgcgag atggatgaac tgctccgtta caacatcacc cgcctggaaa
      961 gccagaccgg cctccgctac ggcgatccgg aggcgccgct gctcttttcc gtgcgttccg
     1021 gcgcgcccat gagcctgccc ggcgccatgg ccaccttcct caatatcggc ctcaacgacg
     1081 agatcaccct caaactcagc acccgcccca attacggctg gacggcctgg gactgctacc
     1141 gccgcctcat ccagagctgg ggtatggctt acggcatcga ccgcgacgaa tttgacgcag
     1201 tgatgatctc cttcaagcgc cgccacaagg tgaagctcaa aacccagttc acgcccctgc
     1261 agatgcgcca gatgtccgaa tcctacaaaa aggtgctggc ccgccacaag ctggagctcg
     1321 aacaggacgt tttcctccaa gtcaagcagg ccatcatgca cgttctggat tcctggaata
     1381 cggaccgcgc ccggctctac cgccagaagc ttcagatcgc cgacgactgg ggaaccgccg
     1441 tcaccatcca gaaaatggtc ctgggcaata tctgcctgga cagcggcacc ggtgtgctct
     1501 tcacccacgc gccctggaac aaggcgccgg ggatcaccct caacggcgat ttcacccttt
     1561 gcagccaggg cgaggacgtc gtggccggcc tggtgcacac cctgcccatc tccgagcagg
     1621 agcgaaagca gtccaaacag aaggtggaac tctctctgga aaaggatttt ccccaaatat
     1681 acgaccgcct gttcagcctc gccagccatc ttttggacga gcgcggctac ccccaccagg
     1741 aaatcgaatt caccttcgaa agccccgccc gtgaccagct ttacatcctc caaacccgca
     1801 accaggtgat aaccaaagcc cgggcctaca ccgttttcaa cgttcccaag cgcgaactga
     1861 agcttctggg caacggcata ggcatcggca aaggcgtaat caacggcctc atcgccattt
     1921 cccgcgcgga catcgacgcc ctcaaagacc gcgggcagcc tctcatcctg gtgcgcccgg
     1981 acaccgttcc ggaggacatg gaactgcttt tcgactgcca gggactgctc acctcgcgcg
     2041 gaggagtcac ctcccacgcc gctgtcaccg ccatccgcct cggcctggtg ggcatcgtca
     2101 actgccgcca gctcaaggtt tttgaggacc agaacaactg ccgcatcggg gaaaccctgt
     2161 tcaagccggg cgacgagatc gccatcgacg ccggcagcgg tgccatctac ctggggcact
     2221 acccggtgga aaccatttcc agataagaat atacacaaaa tacacaaaaa ggagtatcgc
     2281 atgaacctcg acctgaaaaa caaaaaactg ctcgggatca acgggctggg ccggatcggc
     2341 aaattgctgc tctggaatca gatccacctc cgtaatttcg acggaattgt catcaacttc
     2401 ggccgcgaag tggggaaaaa actggacgat ttcatccagg tgatcgagtc cgactccacc
     2461 tacggcagca tccacaaatt cctcttcgga atggtcggac gccgtgccga gatcaagatt
     2521 ctggatccgg acatgccgct catctccatc gaaggcattc ccgtgaaaat cctccgcaaa
     2581 gcccgtgatc ccaaggacat caactggctg aacgaaggcg tgcgcattgt ggtggactgc
     2641 acgggcaatt ttgtggatcc gaacgccacc acggaacagg gaaaaccttg cctgcgcggg
     2701 catctggcag ccggggcgct caaggttgtc tgctccgccc ccttcaagag caaaaccgcc
     2761 agcgtcacaa gttcgccgga cgccatcacc atgatctacg gcatcaacca ccttaattac
     2821 aaacccgccg agcatcacgt tatctccgct gcaagctgca ccaccacggg ccttgcccac
     2881 atgatcaagc ccctcctgga aaacgaggag acctcaaaca tcatgactgc ctccatgagc
     2941 accatccacg ccgccaccaa cacccaaagc atcctggatt ccgtgcccaa aaccggtgcc
     3001 accgacctcc gcaaatcacg cagcatgctc aacaacatca tcctctccac caccggcgcc
     3061 gccaaagccc tggaactggt gatgcccaaa atcaaatccg tgggcttcat ggccgattca
     3121 gtgcgcatcc ccacctccac ggtctccctg atcaacctaa acatgacctt ccacacaaaa
     3181 ctgaacgaac agggccagcc actggtgaac cgccgcctca taaacgagat ctaccaaaag
     3241 gcggcagaag gtccgcagaa agggctgctg gtctattccg aacgccagaa cgtctccgcc
     3301 gacctcatcg gctccctggc tgccgccacc ctcgaagcgc atgaaaccca cacccgcacc
     3361 agcttcatcc ccgtgccgcc ccaaaccctt gccgcgcttg gcgttcctgc cgaccgtgag
     3421 gtgaccatgc ccgtcaccca cgccaagatt tgcggctggt atgacaacga acttggctcc
     3481 tacacccact gcctctccct tttggtgaaa cacatccagt caaccatgcc ctgagccgta
     3541 aaggccggct aaaccgatac cgcccccgga ttccgggggc ttttttgggg ctcatccaaa
     3601 ccgggctatt ggtcgatgac ctcccactca gcctggagtc cagcgagcct gcgagcttgg
     3661 actacaggaa atattgctgt cgtcgatgct cgttcctcgc tcaacgccag cctccagagc
     3721 cacgtcatcc cggacgtcac cgagccagcg aggtgacatt gatccgggat ccatgaaagg
     3781 ataaggactg gattccgggt caggcccgga atgacgtggg tttagggttg ggtgggggtt
     3841 tataggctat tccgccgatc tggattccgg gtcaagcccg gaatgacgtg ggtttggggt
     3901 gacaggctta tttgctgtcg tcgatgctcg ttcctcgctc gacgccagcg cgtggccgtg
     3961 tgatagcgaa gccgactggc cgttaaggcc ctttcctatt tcccggtcac acctctcgta
     4021 tcatacccgc attcggtgcg agtatcatcc gcggggcgtg ggagagggat ggcctcaagc
     4081 gctcagggtg aggcagacag ggcttcgcga agggcaggcg ataagaggga acccataaaa
     4141 ggaaaaaggt tccgctggcg ccttcgggcg gcggagtgaa gtttcgcttg acaacttatt
     4201 ggttttcgag cattagtgtt tgaacgcggt cttgtccggc cgcgcctgtg gaaactcaaa
     4261 aacaagggtg atggaggttc gatgccatat ccgcccaaga ggctgcctgg cccgcttccg
     4321 gacggcgcgg aggcgttttg ccccgccccg tcgcgcgtcc cgttccccgg catgaggggc
     4381 cgttcttttt gccagccggt ttccacagcc agcagagatg ataaacaaac atggagaata
     4441 tcatgaaatg tcccgtatgc aacgccacca atcccgacca cgccactttc tgcaaatcct
     4501 gcggcaaagc gctgcgcagc aacctgcgca cctgtcccaa cggccacgat tatgacgcca
     4561 gcctgccttc ctgcccgcac tgcccaccgc agcgcacggc gaacctgggc caggcgccca
     4621 ctgccaggat gggcgatgaa gccgacagaa ccaggctcga caccgccaaa cccaggctgg
     4681 gggctggtat ttccgggccc ggagccccgc tcagcgacaa aaccgtaatc ctggaaaggc
     4741 cggaagccgc tcccggcgcc gttccctccg cggaaggaaa accgcgtccc caggtgaacc
     4801 ggatgctcac cggctggctg gtgaccttcg atgtggaccc caacggcctg gatttcaagg
     4861 tctatcaggg ccgccacgtc atcggcagtt ccgcctcctg cgacatccag ctcaagctgc
     4921 cgggcgtttc tgccgaacac gccgtcttgc tgagccgcaa cggcaaattc atcatcgagg
     4981 acaacctttc ctcgaatgga accttcgtga acggcgaaat gatcgaagac aaggtgtatc
     5041 tgaatgaaaa cgacatcatc cggatcgcca ccatagacct gaaactcaag accgtgtgag
     5101 gtgcaagatg cgcagactga tgatcctgct tttgctctgc gcgccgctct gggcgcagaa
     5161 catcagccta aaccacctga aaaccaacac cgacaatttc cccaaaatag tgaacagctc
     5221 cgtgctggcc ctggacaacg ccggcgagcc gctcaagaac ctgaacgccg gcaatttccg
     5281 catctccctg ggcggagcgg cggtggacac cctgctgaac gtgaccacct tcgaaagttc
     5341 cggacagggg ctctccatcc ttgtctgcgt ggatgtttcc ggctcgatga agggcaagcc
     5401 gctggacagc gttaaaaacg ccctgctcag cttcatcggc aaaaagcgcg ccaatgatga
     5461 gatcgccatc ctggcctttt ccgagaaaga ggagctggtc tgcgatttca gcacggacac
     5521 ggaatttctg cgcgagagca tcggaaaact gaaaatactg cccggcgaca cctcacttta
     5581 ttattgcatc gacaaaggcc tggaccgcct gggcgggccc accgccagga atgaagggcg
     5641 ctttatgatc ctcttttccg acggcaagga agacaacccc gccaccgcct acaccatcga
     5701 cggggtgata gaaaaagccc gcgcgaagca aatccccatc ttcacggcgg gctacaccag
     5761 gcaggaaaag cagtatctgc aaatcctgga ccgcatctcc cagcagaccg gcggcagctt
     5821 ttacgacgcc ccggacgccc agagcctgag cgaccatttt ggcaaggtga gccgccagat
     5881 actgggctcc tacgtcctca cctaccccgt gttcggactg gcgggcgacg gcctggaaaa
     5941 agacctgagc atcaccctca cccagggcac ttacaaagcc gaattccccg agcccgtgaa
     6001 agttcgggtg ccggccaacc gggaagccat cagcggtccg gaagagatcg acgcgaaagg
     6061 cggcggaaaa ctttccctgg gctggataat cggcatcatc gccgttttgc ttgccgccgc
     6121 agccggaata ttcttctggc tgaaggacaa agagcagaaa cgcctggctg aacagcgccg
     6181 ccagcaggaa gaggaagagc gccagcgccg ggaacaacag gcccgctacg aagaggaaca
     6241 gcgccggctg gaggaacaaa cccggcgcga agccgaaatc cgggcccagg accaaactcc
     6301 ccgctcccgt gagcacacgg ttattctcag tcccggcacg ggcaccggcg aatcacgcgg
     6361 cgaacagggc ctcagcctgg aagtgatgat gggcagcgaa cagggccgga ccttcaacct
     6421 cggggccgaa ggcgccaccc tgggccgggc gcagggaaac agcatcgtgt tcaacgattc
     6481 caccgtttcc tcccgccacg cgcgcatcta ctggagcgac ggccatttct acatcgaaga
     6541 cctcggctcg ctcaacggcg tttatgtgaa cggccggaaa gtcagcctct gccgtatcga
     6601 acagggcagc accttcaagc tgggttcgaa cgaaggcgtg ttccgccttt actgaggccg
     6661 gcgatgagca gaaaaaccgc ggaaaggctg gtgataagca acctcaccga cgtggggatg
     6721 gtgcgcgaaa gcaatcagga ctattacggc aaatacaacg gcggattcgg tgacctgatc
     6781 ctcgtttgcg acgggatggg cggctacaga ggcggggaag ttgcctccca ggtggccgtg
     6841 gaaaccatct ccgcgcattt ccagggcctc ggggccgaat atgacgagat caacgaactg
     6901 caccgcgcct ttagcctcgc ccagcaaaac atcgcccgcc acgcctccga aaaccccgaa
     6961 ctggctgaaa tgggcactac ggcggtggcg ctgctgatca ggggagagca atactggatc
     7021 gcctggattg gcgacagccg catctacaga aggcgcggag gcagcaccat acagctttcc
     7081 aaagaccatt cgtgggtgca ggggatggtg gaccagggcc tgatcaaagc cgaagacgcc
     7141 caagaccacc ccaaaaagaa catcatcctg cgctccctgg gcccggaacc ccacgcctcc
     7201 gaagcggcgg ggcccttccc catcttccgc ggcgatatat tcatgctctg cagcgacggc
     7261 ctttcggact acttcagcct cgccgaggtg gaccgctacc tggccctgga accacagcaa
     7321 gcctgccgcg cgatggtgga cgaggccaaa cgccggggcg gcaaggacaa catcaccatc
     7381 caggtgctca acgtggtgcg gggaaaggct ttcagaccca cgcccggggg gacaaacctg
     7441 ctcacgctgg ggctagcgct gctggctctg gtgtttctca tctgcggggg aatcaacttc
     7501 caaaggctgg cccgggagga aaaacgacgg aaggaagcag agatagccgc ggtgagcgac
     7561 agtatcgcga tcatcgcccc cgatacgacc gggactgtga atgaaaccgg cgctcccgaa
     7621 accggaaacc ccggcaaaga tagcgggagc cagggcaaaa acacttttcc tccatctaaa
     7681 aaagtgaaca aaccaaatca gggaggacat tgacgatgaa actccggatc ttactgatag
     7741 ttgcgctgct cccgcttttg ctgctgacgg cctgcctttt ggcgacggca agcttcccag
     7801 aggggaaacc gggccggaag gcatcttcgc cggacatccg aaaaggtttt gaacattatg
     7861 agtaattccc gattttattt cagcttccgc tggtggcaca tcccgctgat actgctggtg
     7921 cttgggctgg gcttcctggc ctgggctagc acctgggatt tcgacccgga agtgaagccg
     7981 cacattttcc ccaatccctc cctggacgtg aaaaacaacg cctgggtggg atattgggtg
     8041 cagcccttcg aaatccgctt catcgacagg gacaacgaaa ttcccctcat cctggaaagc
     8101 acggagcccc gcttcgtgaa agcccgccaa accacgggca tcccgctccg gatcaaagca
     8161 ggaggcattg aaaacctccg gctgaagagc ctgctgagga tcagggtcca atccatcgtg
     8221 accgccggac gctggggact gagcaagacc atgcgggtgg acagggaaat gagtttgccg
     8281 gaactgctcg accaaaccat caaactctcc accgatgttt tgccccaact gccataggca
     8341 gatagattcc gattcggttt tctgcctctt ctgcggcaag cgcgaaggca aaagcgccgg
     8401 gcatacgaac taaccctact acggcttttg ccctcctgaa gcgaaagacc ggactcggat
     8461 agcagcagga ttctatcgcc gcaatcg
//
LOCUS       DAOOWR010000024         8464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8326_length_8464_cov_3.662504, whole genome shotgun sequence.
ACCESSION   DAOOWR010000024 DAOOWR010000000
VERSION     DAOOWR010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_8464_cov_3.662504"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..2159)
                     /locus_tag="PLB85_01310"
     CDS             complement(183..2159)
                     /locus_tag="PLB85_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71994.1"
                     /translation="MALLLAGYYYWRTRPELERGQRWLLFLLRFVSLSVLLLLLLSPI
                     LYYIRSLKEKQQILVLTDTSASMDLSGPASGAKKNWLKGPGKELADKFAAAGYELHHY
                     DFATGLELDKDNTLLAPALAELAKKHDFSRVKGVVLLSDGWLRDASLQQVKQLGCPFY
                     AITDTTTAKKADLSVVRAITNRQAYRNEPTLIRAEVNSANYNGPATVKLWQGNSLLGS
                     QSVQLKSGVTANVDFTHRFPKTGFFPFRVEVSAPGLGERSQNNNNWPGAIEVLNDKQR
                     VVVLSDSPAWDNKFTLDAISANPRWEASHYRIRDGQAYSGETAAGSLPVENLAALVLI
                     NQGGLQLTGNLLSYVTSAHKRGVGILWQGLPLPELASVLPLQRSNITASYQGFLTLTP
                     ASAKYPMLSFDAAELKNVPPLDYYYVTASRGAEVLGSIDNAQNSPAIAVGTTGNGKTL
                     ALAFLNLWKWQLQSAGGGYQKLLTNSITWLANTSPSGYEAIYNGSYFLGEEINLRLRA
                     QDDIRALRLDLNPQLTITDASGKEVFRDYLTQSEGEYSARFSLDKAGAYSFQIADKVS
                     GEKSGGRFNIADSSIESRDFDFNLPLLSWLSSDTGGKLFSPGSLKEFQPLPAQTKTIE
                     QRADIPLYRKWWVLAIFILSFCLELFFRRRWGLL"
     gene            complement(2186..3448)
                     /locus_tag="PLB85_01315"
     CDS             complement(2186..3448)
                     /locus_tag="PLB85_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71995.1"
                     /translation="MKRIPLLAALIALCALLPATTFTSRSLLFSDSYMLRARGCDANY
                     WNPALLSKEVGDIWLSGLNTGIFIGNNSFDLDLYNFVTREEYLEDEDKQRILDAIDER
                     VASGAGGQVSLFGFTMGNVALSSSVRVGAKAAFDKKYLELLLYGNGDGSQVFEFTRDD
                     NHVEALSYLDLTVGMGDIRIPLPENIPDIDFGFAVSALAGIGNTTTTHFEGLLSSNLD
                     GLTIHQDLTQLAGAGGYGAKGLIGLHCEPVKNLSVGLTLDNIPGFIRWGLVREEFNLH
                     FAADSLYALDLLDEEAEAHYTSSFEQTKADPFNTVFPMEMRLAAMYKTKQVSLSADYI
                     QGFGDSPEISRQGRIALGAELRPIPVLSFFLGYGSPNDSYPWRTSFGIGLNFKELEFG
                     FGIQSIEHFYPGYSSKGLAFGTYVNIRT"
     gene            complement(3445..4986)
                     /locus_tag="PLB85_01320"
     CDS             complement(3445..4986)
                     /locus_tag="PLB85_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU71996.1"
                     /translation="MKKVLILFLGAMLLLGACDINMKPRLPGWDVDLTLPLLNERFLV
                     SELVDSVNVVVGTNDILTITTTGEASTPEFGMVTFNPNSSEDNLNLQSGVNPEIALSL
                     ADPTGAVAISYGHVASGRLEFFIDLIDPAQTTVLVTLPDVTMPDGTPMIISSNDNPNW
                     RSVDLSGYRLGVQNSGTVVEYLRINMQVQSNQPHMTPVGTCGYRLNSNISSDYFEGYL
                     YNYERAVDGTYATIDIDYPEDFDQAVGLQEAIIYLHLNNEFGFSATFHGQVHSVNEKT
                     GEERWVNVLDDNGQPFEVEAATQEGPSITELSFTNGISEVMQIMPNRVELVNGYLLIN
                     GGSGGVPGFVSSTNRLHCTYQVDMPCHFIVHEHVFTMQRPSEVTVSAETQNQIQNRLL
                     DAALTLEVVNRIPLGATATLYVGNTEQIDPTDPGSYCFSKQLTLQPSGYVGPDVDANG
                     AQIIQLTLNETELQVFGNPRVYVLASMSLQESLVPVTIYASPADYIQIRGMLTAKVKV
                     SGDLP"
     gene            complement(5322..6923)
                     /locus_tag="PLB85_01325"
     CDS             complement(5322..6923)
                     /locus_tag="PLB85_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOU71997.1"
                     /translation="MTAQAPQWLWAVGAGGTGGDAGESIEIDSQGNQYVTGYFSDTVT
                     FGSQTLSSNGDLDIFVAKLDPSGNWLWAAQAGGTDRDCGFDISLDSAGNAYVVGYFKY
                     AATFGSHTLTANGYWSSDIFVAKLDPNGNWIWAVNAGGTEYDVGFGIALDGAGNAWVT
                     GYFGGTATFGNVTLTTNGYYDIFVAKLDSSGNWLWAVKAGGTESDEGTNIIVDGMGNA
                     WVTGMFKETTTFGSHTLTASGQFDSFVAKLDSSGNWLWAVKAGGTESDEGNGIAVDGV
                     GNAYVTGYFGDTTTFGSQTLTSSGSDDIFVAKLDSSGNWIWAVQAGGISIDRGYDIVV
                     DGEGNAWVTGHFYGTATFGSHTLTASGGAYDGDMFAAKLDPSGNWIWAAQAGGISNQG
                     GNGIVVDGAGNAYVTGYFGGTTNFGSQTVTSSGGNDIFVAKLGNVTSIEDVLAPEAVA
                     RLHDAWPNPLNRGACALIKADITERSKGILSIFNLRGQSVARYELGPGTHQISLSGEK
                     LPAGVYLYSLQCGAFRETKKLVLLK"
     gene            complement(7164..>8464)
                     /locus_tag="PLB85_01330"
     CDS             complement(7164..>8464)
                     /locus_tag="PLB85_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOU71998.1"
                     /translation="FADTATFGSHTLTAGGTTYNTNIFVAKLDPNGNWLQAVQAEGAS
                     NGSGTDIVLDIAGNAYVTGTFYGTATFGSHTLAADGSGYGDIFVAKLNASGNWLWAVK
                     AGGTGGDEGRGIALDGADNTYVTGSFRGTATFGNYTFTATGLDDLFVAKLNPSGNWIW
                     AVRAGASDDEHVMGNGIAVDGAGNAYVTGKFGGTTTFGSHTLTADGLNIFATKLDPSG
                     NWIWAVKAGGTGWDLGNGIAVDGAGNAWVTGCFEGIATFGSHILTASGGEWDTDVFAA
                     KLDPSGNWIWAVKAGGTEWDRGNGIAVDGAGNTYLTGEFQDTATFGSHTLTSNGFYDI
                     FVVRIGATTSAGIPIAPENLSISTNGTDILLDWDDVTVDTAGNQVSVDHYLVYYCSTG
                     PEGPFTIFGEDSSITQPQWTHSGAASLSPGFYYVTAVVAD"
BASE COUNT         1762 a         2328 c         2413 g         1961 t
ORIGIN      
        1 caggctccaa ggccacgtca tcccggacgt cacagagctt gcgaggtgat attgatccgg
       61 aatccagtat acgaggggct cacgttccct cgctatcctt atgtcaccct acgggctcga
      121 tcaaccggct cgccctcccg gtttgaaagg gagccgtcat tttgtcgcgg taaattaacg
      181 ggtcagagca gtccccatct gcggcggaag aagagttcca ggcagaagga gagtatgaag
      241 atggccagca cccaccattt gcggtagaga ggaatgtcgg cgcgctgttc gatggttttg
      301 gtttgggccg gcaggggctg aaactctttc agggagcctg ggctgaaaag ttttccacct
      361 gtatccgaac ttagccacga gagcaggggc aggttgaaat cgaaatcgcg ggattcgatg
      421 gaggaatcgg ctatattgaa gcgcccgccg ctcttttccc cactcacttt gtccgcgatc
      481 tggaagctgt aggcaccggc cttgtccagg ctgaagcggg cggaatactc accttcgctt
      541 tgggttaggt aatcgcggaa gacctccttg ccggaggcgt cggtgatggt aagctgggga
      601 tttaggtcga ggcgtagggc gcggatgtcg tcctgggcac ggagacgcaa gttgatttcc
      661 tcccccagga aatagctgcc gttatagatg gcctcgtagc cgctgggcga ggtgtttgcc
      721 agccaggtga tggagttggt cagcaatttc tggtagccgc cgcccgcgct ctggagctgc
      781 catttccaga ggttgaggaa ggccagggcc agtgttttgc cgttgcctgt ggtcccgacg
      841 gcgatggcgg gggagttttg ggcgttgtct atgctgccca gcacctccgc tccgcgggaa
      901 gccgtcacat agtaatagtc aaggggtgga acatttttca gttccgcggc gtcgaaactg
      961 agcatggggt atttggcgga ggcgggggtg agggtgagaa acccctggta ggaagcggtg
     1021 atgttggacc tctgcagggg cagaaccgag gccagttcag gcagaggcag gccttgccag
     1081 aggatgccca cgccgcgttt gtgggcggaa gtcacgtaac tgagcaggtt tccagtcagt
     1141 tggaggccgc cttggttgat caggaccagc gcggcgaggt tttccacggg caggcttccg
     1201 gcagcggttt cgccggaata ggcctggccg tcccgaatcc ggtaatgcga ggcttcccag
     1261 cggggattgg cgctgatggc gtccagggtg aacttgttgt cccaggcggg gctgtcggag
     1321 agcaccacca cgcgctgttt gtcatttagc acctcgatgg cgccgggcca gttgttgttg
     1381 ttttgtgagc gttcgcccag gccgggggcg gagacctcca cccggaaggg gaaaaagccg
     1441 gttttgggga agcggtgggt gaaatcaacg ttcgcggtaa ccccgctttt caattgcacg
     1501 ctttggctgc cgagcaggga attcccctgc cagagcttca cggtggcggg gccgttgtag
     1561 ttggcggaat taacctcggc ccggatgagg gtgggctcgt tgcggtaagc ctggcggttg
     1621 gtgatggcgc gaactacgct caaatcggcc ttcttggctg tcgtggtgtc cgtgatggcg
     1681 taaaaagggc atccgagctg ctttacctgc tgcagagaag cgtcgcgcag ccagccatcc
     1741 gagagcagca ccactccttt cacccggctg aaatcgtgct ttttggccag ttccgccagt
     1801 gccggagcca gcagggtgtt gtctttgtcc agttccagcc cggtggcgaa atcgtaatgg
     1861 tgcagctcgt agccggcagc ggcgaattta tctgccagct ctttgcctgg gcccttcagc
     1921 cagttctttt tggcgccgga agccgggccg ctgaggtcca tgctggcgga ggtgtcggtg
     1981 agcaccagga tttgctgttt ctctttgagt gaacggatgt aatagaggat agggctcaac
     2041 agcaacagca gcagcacgga caggctcacg aagcggagca ggaagagcag ccagcgctgg
     2101 ccgcgctcca gctcgggccg ggtgcgccag tagtagtagc ccgccagcaa caaggccacc
     2161 aggatgatga cggcgtaggt caggttcagg tccttatgtt gacataggtt ccaaacgcca
     2221 gccccttgga ggaatatccc ggataaaaat gttcgatgga ctggattccg aagccgaact
     2281 ccaactcttt gaagttcaac ccgatgccaa aggaagtgcg ccaggggtat gaatcattgg
     2341 gagaaccgta acccaggaag aaggacagaa cgggtatggg gcgcagttcc gcgccaaggg
     2401 cgatgcggcc ctggcggctg atctctgggg aatcgccaaa accctggatg tagtctgcgg
     2461 aaagggagac ctgcttggtt ttgtacattg cggcaaggcg catttccatg gggaaaacgg
     2521 tgttgaaagg atcggccttc gtctgttcga aagaggaggt gtaatgggct tcggcttctt
     2581 catccagcag gtccagcgcg tagaggctgt ccgccgcgaa atgaaggttg aattcctccc
     2641 ttaccagtcc ccatctgatg aagccgggga tgttgtccag ggtcaaaccc acgctgaggt
     2701 ttttcaccgg ctcgcagtgg agaccgatca gccccttggc gccatagcct ccggcgccgg
     2761 ccagttgggt gaggtcctgg tggatggtga gtccgtccag attggaggag aggaggcctt
     2821 caaagtgtgt ggtggtggtg tttccgattc cggccagagc gctcacggca aagccaaagt
     2881 cgatatccgg aatgttttcg ggcaggggga tccggatatc gcccatcccc acggtcaggt
     2941 ccagatagct gagcgcctcc acgtgattgt catcccgcgt gaattcgaag acctggctgc
     3001 cgtcgccgtt gccgtagagc agcaattcca gatacttttt gtcgaacgcg gctttggcgc
     3061 ccacacgcac ggagctgctg agcgccacgt tgcccatcgt gaaaccgaag agggaaacct
     3121 gcccgccagc gccgctggcg accctttcgt cgatggcgtc caggatccgc tgtttgtcct
     3181 cgtcttcaag gtattcctcg cgggtgacga agttgtagag gtccaggtcg aaggaattgt
     3241 ttccgataaa gatgcccgtg ttcagcccag aaagccagat atcgccgact tccttgctga
     3301 gcagggcggg gttccagtag ttggcgtcgc agccccgcgc ccggagcata tagctgtcgg
     3361 aaaacagcag tgacctggag gtgaaggtgg tggccggcaa caaggcgcac agcgcgatca
     3421 gggccgcgag cagaggtatt cgtttcacgg caggtcccca gaaaccttaa ccttcgcggt
     3481 gagcattccc ctgatttgga tgtaatccgc cggtgaagcg tagatggtca ccggaaccag
     3541 gctttcttgc aggctcatcg aggccagcac gtaaacccgg ggattgccaa aaacctgaag
     3601 ctcggtttcg ttaagggtta gctggatgat ctgcgctcca ttggcatcca catccgggcc
     3661 cacatatccg gatggttgca aggtaagctg tttggaaaag cagtagctgc cggggtcggt
     3721 gggatcgatc tgttcggtgt tgcccacata gagggtggcg gtggcgccca gggggatgcg
     3781 gttcaccact tccagggtca gggctgcgtc aagcaaccgg ttctgaattt ggttctgagt
     3841 ttcggcggaa accgttactt cagacggccg ctgcatcgtg aaaacgtgtt cgtgcacgat
     3901 gaagtggcag ggcatatcaa cctggtaagt gcaatgcagg cggttggttg agctcacgaa
     3961 accgggcacc ccgccggagc cgccgttgat aagcaggtat ccgttcacca gttccacccg
     4021 gttgggcatg atctgcatca cctcgctgat gccgttggtg aagctcagtt ctgtgatgga
     4081 aggaccttcc tgggtggcgg cttcaacctc aaagggttgc ccgttgtcat ccaaaacgtt
     4141 cacccagcgc tcctcgccgg ttttttcgtt tactgagtgg acctggccgt ggaaagtggc
     4201 ggaaaagccg aactcgttgt tgaggtgcag atagattatc gcttcctgga gtcccaccgc
     4261 ctggtcgaaa tcctcaggat aatcgatgtc gatggtggca taggtcccgt ccacagcgcg
     4321 ctcgtaattg tagaggtagc cctcaaaata gtcgctgctg atattgctgt tcaggcggta
     4381 gccgcaggtt ccgacgggcg tcatgtgggg ttggttggat tgcacctgca tgttgatgcg
     4441 caggtattcc accacggtgc cggaattctg cacccccagt ctgtagccgc taaggtccac
     4501 ggagcgccag ttcgggttgt cattgctcga gatgatcatg ggtgtcccat ccggcatggt
     4561 aacatccggc agggtgacca aaaccgtggt ttgcgccggg tcgatgaggt caatgaagaa
     4621 ttccagcctg ccgctcgcca catgaccata ggaaatggcc accgctccgg tggggtcggc
     4681 aagagaaagg gcgatttcag gattgactcc ggattggagg ttcagattat cctcggagct
     4741 gttcgggttg aaggtaacca tgccgaattc cggggtggag gcctctccag tcgtggtgat
     4801 ggtgaggatg tcattggtcc ccaccacaac gttcacgctg tccaccagtt cggaaaccag
     4861 gaaacgctcg ttaagcagcg gaagcgtcag gtccacgtcc caaccaggca accgcggttt
     4921 catattgatg tcacaggcgc ccagaagtag catcgcgccc aagaaaagaa tcagtacttt
     4981 tttcatcggt tcaccatcct ttctgccttg gcgggcagat ttgtattatt tgcttcgaag
     5041 atactcttct ctgcatcctg atctgttgtc aagctttttc ctcgcctggg gtgaattatc
     5101 tttcaaaccg gcttaagcag gcaaagatat cgaggctggt tccgcctttc agtctgtagc
     5161 ctggcgtcca gcggacgttg tgagcatgga ttataggaag taatggaaga gtcattcatt
     5221 gctgacgtcg atgcttgttc ctcgctcgac gccagcctct ccgccaacta tttctccttt
     5281 tctctcttcc caaattcgcg taacttgtgg acacccccac actatttcag caacaccagt
     5341 ttcttggttt ccctgaatgc cccgcattgc aggctgtaaa gatagacccc cgcgggaagc
     5401 ttttcaccgc ttaaggatat ctggtgcgta ccggggccga gttcatagcg ggccacgctt
     5461 tgcccgcgca ggttaaagat gctcaggatg cctttcgacc gctcagtaat gtccgccttg
     5521 atcagcgcgc aggcgcccct gttcagggga ttgggccagg cgtcatgcag tctggctact
     5581 gcttccggcg ccagaacatc ttctatcgat gtgacgtttc ccaacttggc gacaaagata
     5641 tcgttacctc cgcttgaggt tactgtctgg ctgccgaaat tggttgtccc tccaaaatac
     5701 ccggtcacgt atgcgtttcc agcgccatcg acgacaatgc cattcccccc ttgattgctt
     5761 atccctccag cttgggcagc ccagatccag ttgccggagg ggtccagctt agcggcgaac
     5821 atatctccgt catatgcccc tccacttgca gtcagggtat ggctgccaaa agtggcggta
     5881 ccataaaaat gcccggtcac ccaagcgtta ccctcgccat ccacgactat atcatatcct
     5941 ctgtcaatgc ttattcctcc agcctggacc gcccagatcc agttgccgga agagtcaagc
     6001 ttggcaacaa agatatcgtc gcttccgctt gaggttagtg tctgactacc aaaagtggtt
     6061 gtatctccaa aatacccggt cacatatgcg tttcccacac catccacggc aatgccattt
     6121 ccctcgtcac tttctgttcc tccggctttg accgcccaga gccagttgcc tgaagagtca
     6181 agcttggcga cgaaactgtc aaattgtcca cttgctgtca gggtatggct gccgaaagtg
     6241 gtggtttctt taaacatccc ggtcacccag gcgttaccca tgccatccac aatgatgtta
     6301 gttccctcgt cactttctgt tcctccggct ttgaccgccc agagccagtt gcctgaagag
     6361 tcaagcttgg cgacgaagat atcatagtat ccgtttgtcg tcagtgtaac gtttccaaaa
     6421 gtcgctgtgc ctccaaaata tccggtcacc cacgcgtttc ccgcaccatc cagagcgatg
     6481 ccaaaaccca cgtcatattc tgttcctccc gcattaaccg cccagatcca gttgccgttt
     6541 ggatctagct tggcaacaaa gatatcgctg ctccaatagc cattggccgt cagtgtatgg
     6601 ctgccgaaag tggctgcgta tttaaaatac ccgaccacat aggcattacc cgcgctatcc
     6661 agagagatgt caaaaccaca atcacggtct gttcctccag cttgggctgc ccagagccag
     6721 ttgccggagg ggtccagctt ggcgacaaag atatctaagt ctccatttga cgacagggtc
     6781 tgactgccga aagtgacggt gtcagaaaaa tacccagtaa catactggtt gccctggctg
     6841 tcaatttcta tggattcgcc tgcatccccg cctgtccctc ccgcgccaac cgcccacagc
     6901 cattgtggtg cctgtgctgt caaaacagtc gctccacaga gcaggacaaa atataaaaaa
     6961 aagcttggaa ccgtcttcat ttttcctccc gaagaccttt ttttattaag ggggacacat
     7021 tgacgtggta ttgcaaaatg tatcaagcac aagaaccttt tcctttgaac taaacccggg
     7081 aaacagtgat gagcaaggag gactatcttg cttcagcctg acgataccgg ctggtcgctc
     7141 acaggttatc tatacgtgtc ctctcagtca gcaactaccg ccgtaacgta gtaaaagcca
     7201 ggcgaaagcg aggccgcgcc gctatgtgtc cactgcggtt gtgtgattga gctatcctca
     7261 ccaaatatcg tgaagggtcc ttcaggaccg gtggagcaat aatagaccag atagtggtcc
     7321 acactcacct gattacctgc tgtatctact gtaacgtcat cccaatccag caggatatcc
     7381 gtgccgtttg tgctaatgct caggttttcc ggagctatgg ggatccctgc actcgtagta
     7441 gcgccgatcc tgacgacaaa gatatcatag aatccgtttg atgtcagggt atggcttccg
     7501 aaagtggctg tgtcttgaaa ctctcctgtc aaataggtgt ttcccgctcc atccacagcg
     7561 atgccatttc cccggtccca ttctgtccct cccgccttga cagcccaaat ccagttgcca
     7621 gaggggtcca gcttggcggc aaatacatct gtatcccatt cgccaccgct tgctgtaagg
     7681 atatggcttc cgaaagtggc tatgccttca aaacatccgg tcacccaggc gtttcctgct
     7741 ccatccacgg cgatgccatt tcccaggtcc catcctgtcc ctcccgcttt gacagcccag
     7801 atccagttgc cagaggggtc cagtttggtt gcaaagatat ttagaccgtc tgctgtcagt
     7861 gtatggctgc cgaaagtggt tgtacctcca aacttcccag tcacataggc attacccgct
     7921 ccatccacgg cgattccatt tcccattaca tgttcgtcgt ctgacgctcc tgcacggaca
     7981 gcccagatcc agttgccaga gggattaagc ttggcaacga ataaatcatc aagtccggtt
     8041 gccgtaaagg tatagtttcc gaaagtggct gtgcctctaa aactcccggt cacataggtg
     8101 ttatccgctc catccagggc gatgccacgt ccctcgtcac ctcctgtccc tcctgctttg
     8161 acagcccaga gccagttgcc ggaggcattc agcttggcga caaagatatc gccataccca
     8221 gagccatctg ctgccagtgt gtgactgcca aaagtggctg tgccataaaa agtaccggtc
     8281 acgtaagcat tgcccgcgat atccaggact atgtccgttc cagatccatt ggatgcccct
     8341 tccgcttgga cagcctggag ccagttgcca ttggggtcca gcttggcgac aaagatattg
     8401 gtattataag tagttccgcc tgccgtcagt gtatggcttc cgaaagtggc tgtgtctgca
     8461 aaac
//
LOCUS       DAOOWR010000025         8208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8695_length_8208_cov_3.680854, whole genome shotgun sequence.
ACCESSION   DAOOWR010000025 DAOOWR010000000
VERSION     DAOOWR010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8695_length_8208_cov_3.680854"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLB85_01335"
     CDS             <1..493
                     /locus_tag="PLB85_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOU71999.1"
                     /translation="AIGMSMQGTLGQFASGVMLIGLRPFKLGDTVVAGGQTGTVHDIG
                     ILSTTILTSDNKKIIVPNSAIVGGPITNFSAMPTRKIELAIVVPGTTDLNKARDILKG
                     ILEAESRVLKDPAPSITIADASAAEIKYGIGAHVNNTDMAAVQASLLENIKQALTAAG
                     IWA"
     gene            507..1352
                     /locus_tag="PLB85_01340"
     CDS             507..1352
                     /locus_tag="PLB85_01340"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU72000.1"
                     /translation="MKNIRFILAAALLLSFALLFAEAWKFDSDIMVNLTQSQFSDNWA
                     GSELSNITWTASMNNTAQKQLAEWLKNKNTLKLAFGQTHLQKEDLLGETYWEKPQKST
                     DQIAFESLMQFTLKTFVDPYLSLRADSQFLDEAGTKTLVVNPILFTESAGIMKTIVDG
                     EKTKLNARIGGAVRENWNRRVDGIPVDGGIEGIVEFKQIFGLLNASYNSRLSAYKALF
                     KSDAVAGVDNWKAPDLKWENLLSLNLWKMLSLNLNLDFIYEKEQSPDIQWKEILGLGF
                     SYSLF"
     gene            1922..4123
                     /locus_tag="PLB85_01345"
     CDS             1922..4123
                     /locus_tag="PLB85_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72001.1"
                     /translation="MTRSTIILCAVLLLGTMALIAQGGEWEYFSYGGQIRSFAHHADD
                     VWIGTTTGLVRYDTITQLKQFINKSNSPLSNNLILSVAVSPSGILWIGTSKGLYRVEG
                     ENWQYFDSQNSGLPTNAAREIFCLSDTELWLLMGNNGGNDYACHYVGGTFVPYSAGSD
                     PIWGQVIRGMALDQQGVPWLGWYNTSNGTFGISHFADSFWISQSMSDLGLPNQDIFGL
                     AHDGSRLWLGTGTGQLFSIDSGGAQIHDLTQPPYNMHVVTDMAVDSQNRLLAAFRAWD
                     GKGYLLRRQGDGWELLDPNQVPHLNSTRAIMEDATGRIWLGTGGGVAIHDGTSWSAFD
                     CSNSPLPTNNLGCLTIDHQGDLWMSLHDFQGGTSALAKKSGDNWTFMYSTEYSYLRNP
                     HELAFSPDGTMWFRDNYFGGIVSFDGTNWTRYHLIFQNLPEGTLNLLRLDGNGIPWIT
                     ISSQDYQTRVYRLEQGAWAEKAVLLPGVLDMAFDGVNNAWLATTGGLVYLGDSTEIYN
                     TQNSGLTNNQVQCLAIGADRKLWIGTSSGLCSYLNGVWETWDISHGNHPFRCFLDLVA
                     APDGRVWGATQNTGLVCFDGTEFSVFMENNSPLTYNRIKELELDPQGNLWMDSLGQGI
                     IRFHPSTTSANDPAQVPPQLPCLSNFPNPFNPSTTIRFTLPESGPASLGIYNLRGQLV
                     RELCSSNLKDGEHSYVWDGRDSRGNDVSSGVYFARLKTGTNSRSHKLLLMK"
     gene            4233..6191
                     /locus_tag="PLB85_01350"
     CDS             4233..6191
                     /locus_tag="PLB85_01350"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOU72002.1"
                     /translation="MTKKLLLLLVVAVLLSFSLAKSQETGTIKGRVSSHDGKPLANVK
                     IECYLDYQRVAVVSSDSQGKFVARGLIPGKYLVHASLEGYELWKGDNIYVKTGRTAIL
                     NISLKPGILVPETVVEPEIVELQSGVNKVGEIHTRGGRTGEVHMSLMDMGDGSYSLAP
                     LPGQYPPDWNTENYASITPNIFHSPLADPLSTFSIDVDTATYSNVRRQLNQGRLPTPN
                     TVRTEEILNYFNYDYPQPQGETPFSIYTEMGICPWNQKRNLVHIGLQGKKIDMSKAPA
                     NNLVFLLDVSGSMDHPNKLPLVKKSMELLLENLRPQDRVAIVVYAGAAGLVLPSTSGE
                     EKAKISAAMNNLEAGGSTAGGAGIKLAYKTAVENLIPGGNNRIILCTDGDFNIGVSSN
                     ADMTKLIEENRNNGVFLTVLGFGMGNYKDDRLELLADKGNGNYAYIDDIMEAKKVLVN
                     ELGATLYTIAKDVKIQVEFNPAHVKYYRLIGYENRLLRDEDFKDDTKDAGELGAGHSV
                     TAIYEIVPANSKEEIPVPDSLKYQTTTITEESRNSPEVMTVKLRYKLPDGDTSIPLET
                     PVYNKTLGLKDVSHTFGFSAAAVGYAMMLQNSEHKAALTWDMVRALATENLGADTDGY
                     RKEFLTLIDKAQTLMEKSQKIMEENRPD"
     gene            complement(6311..6955)
                     /locus_tag="PLB85_01355"
     CDS             complement(6311..6955)
                     /locus_tag="PLB85_01355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOU72003.1"
                     /translation="MSRALLQHLELLRNLGVTEIYRPAPAAGEDGPDLESLRQLHSNC
                     QNCPLGATRNHFVYGEGNPRARAMLIGEAPGAEEDRTGRPFVGDAGKLLSDMLRAIEL
                     KREDVYICNILKCRPPNNRDPGPVERAACMPLLLEQIETIQPRIILMLGRVAAQTLLN
                     SNLTLEKLRGVVHTFQGIPSYVTYHPAALLRNTHWKRPAWADLQKFRDHYRSLD"
     gene            complement(6965..7165)
                     /locus_tag="PLB85_01360"
     CDS             complement(6965..7165)
                     /locus_tag="PLB85_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72004.1"
                     /translation="MSSNEVPDYFAEIEEANEMMAKMASPFADDEADMGEETFDDSEK
                     HIEELMHDNLDRYSDDEDDEEE"
     gene            complement(7310..7942)
                     /gene="gmk"
                     /locus_tag="PLB85_01365"
     CDS             complement(7310..7942)
                     /gene="gmk"
                     /locus_tag="PLB85_01365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOU72005.1"
                     /translation="MITKNKNFLIILSAPSGGGKSSILREVLKAHPGIDYSVSYTTRP
                     PRGQEKDGEHYHFVDEQSFLARQEEGDFLESASVFGNWYGTSVSFIQSRLAAGRHVIL
                     DIDVQGAAQISATTIPYVKIFIVPPSMEILRQRLTERSTDSPREIAKRLTIARQELEF
                     IPAYDYLVVNDRLSDAVADVIAIIRAEENSVSRYREPIAGFLEQGEKHDR"
     gene            complement(7956..>8208)
                     /locus_tag="PLB85_01370"
     CDS             complement(7956..>8208)
                     /locus_tag="PLB85_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOU72006.1"
                     /translation="PDAGAKVSAGEPCGTIEAVKSAEDLISPVSGVLENKNQEAEDNP
                     EIINQSPYEEGWLYRVRMNNEEELANLLTPIEYKRLIEG"
BASE COUNT         1844 a         2438 c         2270 g         1656 t
ORIGIN      
        1 ggccatcggc atgtccatgc agggcacgct gggccagttt gccagcgggg tgatgctgat
       61 cgggctgcgt cccttcaagc ttggcgatac agtggttgct ggcggccaaa ccggcacggt
      121 gcacgacatc ggcatcctca gcaccaccat cctcaccagc gacaacaaga agatcatcgt
      181 gccgaacagc gccatcgtcg gcgggcccat caccaatttt tccgccatgc ccacccgcaa
      241 gatcgagctg gccattgttg tgcccggaac aaccgacctg aacaaggccc gcgacatcct
      301 caaaggcatt ctggaagctg aaagccgcgt cctcaaagac cccgcccctt ccatcaccat
      361 cgccgacgcc tccgccgccg agatcaaata cggcatcgga gcgcatgtga acaacacgga
      421 catggctgcc gtccaggcat ccctgctgga aaacatcaaa caagccctca ccgccgccgg
      481 catctgggcc taaaggaaac aatgacatga agaacatcag atttatccta gccgcagccc
      541 tgctgctgtc tttcgccctc ctcttcgccg aagcctggaa gttcgattcc gacatcatgg
      601 tgaacctcac ccaaagccag ttcagcgaca actgggccgg cagcgaactg agcaacatca
      661 cctggaccgc ctccatgaac aacaccgccc agaaacaact ggccgagtgg cttaaaaaca
      721 agaacaccct caagctcgcc tttggccaga cccacctcca gaaagaggac ctgctgggtg
      781 aaacctactg ggagaaaccc cagaaatcca ccgaccagat cgccttcgaa tccctgatgc
      841 agttcaccct gaaaaccttt gtggacccat acctatccct gcgtgcggat tcccagttcc
      901 tggatgaggc cggcaccaaa acccttgtgg tcaatcccat cctcttcacc gaaagcgccg
      961 gtatcatgaa gaccatcgta gatggcgaaa agaccaagct gaacgcccgg atcggcggcg
     1021 ccgtccgcga aaactggaac cgccgcgttg acggcatccc cgtggatgga ggcatcgagg
     1081 gaatcgtaga gttcaaacag atcttcggcc tgctcaatgc cagctacaac agccgcctca
     1141 gcgcctacaa ggccctcttc aaatccgacg ccgtggccgg agttgacaac tggaaagcgc
     1201 ccgacctcaa gtgggaaaac ctgctcagct taaacctctg gaagatgctg agcctgaacc
     1261 tgaacctcga ttttatctac gaaaaggaac aaagcccgga tatccagtgg aaggaaatcc
     1321 tgggcctcgg cttcagctac tctctgttct aaaccaagct cttttccaaa tcccgcgccc
     1381 ggaccggctt tgctgccttc gctgccgggg aaacctggag tccagcgagc ctgcgagctt
     1441 ggactacagg atgtgttgct gccttcgctg cccgtccctc gctcgacgcc agcgtgtgtc
     1501 catgctgacc ggccgtgaag gccctgtcct gtgtttgggt agcacctccc gcacaatgcc
     1561 cgcattcggt gcggggattg tgcaggaggc gtgggagggg gaaaggctca agcgctaagg
     1621 gcctggccga cgggaaaccc ggttgattga gcccataggg cggaataacg atagcgaggg
     1681 agcgcaagcc cctcgcacac tggattcagg gtcaagccaa gaatgacgtg gtttttttgg
     1741 ggtagtatgt tgacaagggc aataacaggg attaccgggg gtccgtgaaa acaatccaca
     1801 gcgccagccg gaacggaggc gatttcccct tgacatattt tccaagcacc gcaaagttgt
     1861 cacgcatgcc ttggagcgga tcgctttttg tctttaccat aaaagcaaag acgaggaaaa
     1921 aatgactcga tcaaccatca tcctctgcgc ggtcctgctg cttggcacta tggcgcttat
     1981 agcccagggc ggggagtggg aatacttcag ttacggcggt cagatccgct cttttgccca
     2041 ccacgccgat gacgtgtgga ttggcactac aaccggattg gtgcgatacg acaccatcac
     2101 ccagctaaaa cagtttatca acaaatccaa ctcccctctc agtaacaacc tgatcctttc
     2161 tgtggcggtg tctccctccg gaatcctctg gatcggcacc agcaaaggcc tctaccgggt
     2221 ggagggtgaa aactggcagt atttcgacag ccagaacagc ggcttgccca ccaacgctgc
     2281 gcgcgagata ttttgcctgt ctgacaccga gttgtggctg ctgatgggta ataacggcgg
     2341 aaatgactac gcctgccact atgtgggggg aacctttgtg ccctactccg cgggcagcga
     2401 ccccatctgg ggccaggtga tcaggggcat ggcgctggac cagcagggtg ttccctggct
     2461 gggctggtac aacacaagca atggcacttt cggcatctcg cactttgccg attctttctg
     2521 gatcagccag tccatgtcag atctgggcct gcccaaccag gacatcttcg gccttgccca
     2581 cgacggcagc cgtctctggc tgggaaccgg cacaggccaa ctgttcagta tcgactctgg
     2641 aggcgcgcaa atccatgatc tcacccagcc tccctacaat atgcacgttg tcaccgacat
     2701 ggcggtggac agccaaaaca ggctgctggc cgccttccgt gcctgggatg gaaaaggata
     2761 cctgctaaga aggcaggggg acggctggga attgctggac cccaaccaag tgcctcatct
     2821 gaattcgacc cgggcaatca tggaagacgc cacgggcagg atttggctgg gaaccggcgg
     2881 cggggtggcc atccatgacg gaacctcctg gagcgctttc gactgctcga actcccctct
     2941 gcccaccaat aacttgggct gcctgacgat cgaccaccag ggcgaccttt ggatgagctt
     3001 gcacgatttt cagggaggca catccgccct ggcgaaaaaa agcggggata actggacttt
     3061 catgtacagc acggaatact cctacctccg caatcctcat gagttggcct tcagcccgga
     3121 tggaaccatg tggttcaggg acaactactt cggaggtatc gtcagttttg acggaaccaa
     3181 ttggacacga taccacctca ttttccaaaa cctgcccgaa ggtacgctca acctcctcag
     3241 actagacggc aacggcatac cctggattac catctccagt caggactacc agacccgcgt
     3301 ttaccggctg gagcagggag cctgggcaga aaaggcagtg ctgctgcctg gtgtgttaga
     3361 catggccttt gacggagtga acaatgcctg gctggcaacc actggcggct tggtttatct
     3421 gggagacagc accgaaatct ataacaccca gaactccggc ctgacgaaca atcaagtcca
     3481 atgcttggcc ataggagcgg acaggaaact ctggataggc acctcatccg gcctttgcag
     3541 ctatctgaac ggcgtttggg aaacttggga catctcgcac ggaaaccatc ccttccgctg
     3601 ttttttggat ctggtcgccg ctccggacgg acgcgtttgg ggagccaccc aaaacaccgg
     3661 cctggtctgt ttcgacggca ctgaattcag tgtatttatg gagaataatt cgcccctaac
     3721 atacaatagg atcaaagagc tcgagctgga tccacagggc aacctgtgga tggacagcct
     3781 tggacaaggc atcatccgct tccatccctc caccacatca gcaaatgacc ccgcgcaggt
     3841 gccgccgcaa ctaccctgcc tcagcaactt ccccaacccc ttcaatccct ccactaccat
     3901 caggttcacc ctgcccgaaa gcggccctgc cagcctgggc atctacaacc tccggggtca
     3961 gcttgtgcgc gagctctgct cctccaacct gaaggatggt gaacatagct atgtttggga
     4021 tggccgggac agccggggca acgatgtttc cagcggcgtc tatttcgccc gcctgaaaac
     4081 agggacgaac agccgcagcc acaagctgct gctgatgaaa tgacagaaat ccagcccctt
     4141 cccgggccgg cctcaagata attgttgaca gggattccga cctcagtaag tattggtttt
     4201 ggaaccaaat tctgaattcc caaggaggag agatgaccaa gaaacttttg ctcctgctgg
     4261 tggtcgcggt gctgctcagc ttcagcctgg ccaagagcca ggagaccgga accatcaagg
     4321 gaagggttag cagccacgat ggcaagcccc ttgccaatgt gaagatcgaa tgctatctgg
     4381 actatcagcg ggtggcggtg gtgagttcgg attcgcaagg caaattcgtt gcccgcggcc
     4441 tcatccccgg caaatacctt gtccatgctt cgctggaagg ctatgagctc tggaaaggtg
     4501 ataatatcta cgtcaagacg ggccgcacag ccatcctgaa catcagcctc aaaccgggga
     4561 tcctggtgcc cgaaacggtg gtggagccgg agatagtgga gctgcaatca ggcgtgaaca
     4621 aagtgggcga gatccatacc cgcggcggac ggaccggcga ggtccacatg tccttaatgg
     4681 atatgggtga cggcagctac agcttggccc cgctacccgg ccagtatccc ccggactgga
     4741 acacagagaa ttacgcctct atcaccccaa acatattcca cagcccgctg gcggacccac
     4801 tctccacctt ctccatcgac gtggatacag ccacctacag caatgtgcgc cgccagctca
     4861 accagggccg gctgcccacc cctaacaccg tcaggacgga ggagatcctg aactatttca
     4921 actacgacta tccccagccc cagggcgaaa cgcctttctc catctacacc gaaatgggca
     4981 tctgtccctg gaaccagaag cgcaacctgg tgcacatcgg cctccagggc aaaaagatcg
     5041 acatgagcaa agccccggcc aacaaccttg tcttcctgct ggatgtttcc ggctccatgg
     5101 accacccaaa caagcttccg ctggttaaaa aatccatgga actgcttttg gaaaacctgc
     5161 ggccccagga cagggtcgcc atcgtggtct atgccggagc ggcaggttta gtgctgcctt
     5221 ccacttccgg agaggaaaag gccaaaatct ccgccgcaat gaacaacctt gaggccggcg
     5281 gctccactgc cggcggagcg ggcatcaagc ttgcctacaa gaccgccgtg gaaaacctca
     5341 tccccggagg aaacaaccgc atcatcctct gcaccgacgg cgatttcaac atcggtgtct
     5401 cctccaacgc cgatatgacc aaactcattg aagagaaccg caacaatggc gttttcctca
     5461 ccgtgctcgg cttcggcatg ggcaattaca aagacgaccg cctcgagctt ctggccgata
     5521 agggcaacgg caactacgcc tacatcgatg acatcatgga agccaaaaag gtgttggtga
     5581 acgaacttgg cgccaccctc tacaccatcg ccaaggacgt gaagatccag gtggaattca
     5641 accctgccca cgtgaaatat taccgcctca tcggctatga gaaccgcctg ctacgcgacg
     5701 aggacttcaa ggatgacaca aaggacgccg gagaactggg cgccggacac tccgtgacgg
     5761 ccatctatga gatcgtgccc gccaattcca aggaagagat acccgtcccc gactccctca
     5821 aataccaaac caccaccatc accgaggaat cgcgcaactc gcccgaggtc atgaccgtga
     5881 aactgcgcta caaactgccc gacggcgata cctccatccc cctggaaacc ccagtttaca
     5941 acaaaaccct gggcctgaaa gacgtttccc acacttttgg cttctcagcc gccgcggtgg
     6001 gatatgccat gatgctgcaa aacagcgaac acaaggccgc cctgacctgg gatatggtgc
     6061 gcgccctcgc aactgagaac ctcggcgccg acacagacgg ctacaggaag gaatttctca
     6121 cccttatcga caaagcccaa accctcatgg aaaaatccca aaagatcatg gaagagaaca
     6181 gacccgacta accttccact cccatagcgc gcctttggcg taaaccggcg aggtcgatca
     6241 ggtctcgcct tttttgtgtc tttatctgga actggatgtg ggaaaggggg tgaccggaat
     6301 caggcggttc tcaatccagg gagcggtagt ggtcgcggaa tttctgcaaa tcggcccagg
     6361 cggggcgttt ccagtgtgta ttgcgcagca gagcggctgg gtggtaggtc acgtagctgg
     6421 ggatgccctg gaaagtatgc acgacgccgc ggagcttttc cagggtgaga ttgctgttca
     6481 ggagggtttg agcggccacg cggcccagca tgaggatgat gcgcggctgg atggtttcta
     6541 tctgctcaag cagcaaaggc atgcaggcgg cgcgttccac cggcccggga tcgcggttgt
     6601 tgggagggcg gcacttgagg atgttgcaga tatagacgtc ctcccgcttc agttcgatgg
     6661 cccggagcat gtcgctcaac agtttgccgg catctccaac aaaggggcgg ccggtgcggt
     6721 cttcctcggc tccgggtgct tcgccgatca gcatggcgcg ggcgcggggg ttgccctctc
     6781 cgtaaacaaa atggttcctg gtggcgccca gagggcagtt ctgacagttg gagtggagct
     6841 ggcgcaggct ttccaggtcg ggcccgtcct caccggcagc aggggcgggg cggtagattt
     6901 ccgtcactcc aaggttgcgc agcagttcaa ggtgttgcag cagggcgcgg gacatcggga
     6961 cggcttattc ttcttcgtcg tcttcgtcgt cggaatagcg gtcgaggttg tcgtgcatca
     7021 gttcttcgat gtgtttctcg gaatcgtcga aggtctcttc gcccatgtcg gcttcatcat
     7081 cggcgaaagg cgatgccatt ttggccatca tttcgttcgc ctcctcgatc tcggcaaagt
     7141 aatctggcac ttcgttcgat gacacgtgtt ccatagccag cactgatatc ttttggaaaa
     7201 cttctggcgc acgaacacgg gaagctgggt tagcctctgc gcttccagtt tggagagcag
     7261 gcagaaaagg tagttgaggt ctgttttctc cagaaaggat tcgatgtttt tatcgatcat
     7321 gtttttctcc ttgttcaagg aagccggcaa tcggctcccg gtagcgactg acgctgtttt
     7381 cctcggccct aatgatggcg atgacgtcgg ccacggcatc ggacagccgg tcgttcacca
     7441 ccagatagtc gtaagcaggg atgaattcca gctcctggcg ggcgatggtc aggcgtttgg
     7501 cgatttccct gggcgaatct gtggagcgct ctgtgaggcg ctggcgcaaa atctccatcg
     7561 agggcggcac gatgaagatc ttcacgtagg ggatcgtggt ggcgctgatc tgggcggcgc
     7621 cctggacatc gatgtccagg atcacgtggc gtcccgcggc cagccgactt tggatgaagc
     7681 tgacggaggt gccgtaccag tttccgaaga cgctggcact ctccagaaaa tccccttcct
     7741 cctggcgggc cagaaagctt tgctcatcca caaagtggta gtgctcgcca tccttttcct
     7801 ggccccgggg aggtctggtg gtgtaggaaa cggagtagtc gatgcccgga tgggctttca
     7861 gcacttctct caggatggag ctcttgcctc ctccggaagg ggcggagagg atgatgagga
     7921 aattcttgtt tttggtgatc acggcctggc aggggtcagc cctctatgag tcttttatat
     7981 tctatgggag tgagcagatt ggccagttct tcttcgttgt tcatgcgcac ccggtagagc
     8041 cagccttctt catagggaga ttggttgatg atttcggggt tgtcctcggc ttcctggttt
     8101 ttgttttcca aaactccgct caccgggctg atgaggtcct cggcggattt cacggcttcg
     8161 atggtgccgc agggctctcc ggcggaaacc tttgccccgg cgtcgggc
//
LOCUS       DAOOWR010000026         8207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8697_length_8207_cov_3.756869, whole genome shotgun sequence.
ACCESSION   DAOOWR010000026 DAOOWR010000000
VERSION     DAOOWR010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8207
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_8207_cov_3.756869"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1091)
                     /gene="hydE"
                     /locus_tag="PLB85_01375"
     CDS             complement(36..1091)
                     /gene="hydE"
                     /locus_tag="PLB85_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOU72007.1"
                     /translation="MRPNRDELINMLTLSDPAELEALYKRAYEVKREYVGTKVYFRGL
                     IELSNICTKNCIYCGIRCGNPNVKRYQISREEVLAEARWCWEQRYGSLVIQAGEREDK
                     AWSDFITDLVVDIKNLSRGELGITLSLGEQTEKVYRQWFEAGAHRYLLRIEESNPELY
                     KALHPAGHSFQKRLECLRSLRKVGYQTGTGVMIGLPGQTAEHLADDILFFYDEDIDMI
                     GMGPFIPHHETPLAHLIPSYDPEKALEAGLKMIAATRIALRDVNIASTTALQALMSSG
                     RELGLLAGANIIMPNITDTSFRDGYQLYEGKPGLDENAQSSRSALEKSILSIGESIGY
                     AEWGDSRHFKYRNWNDS"
     gene            complement(1456..1743)
                     /locus_tag="PLB85_01380"
     CDS             complement(1456..1743)
                     /locus_tag="PLB85_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU72008.1"
                     /translation="MSRDDLIKKIAGTAGISQKTAGIALNAMLEGVTQALKKEEKVSL
                     VGFGSFSVAHRKARNGVNPKTKKPLKIPARKVPVFKAGKRLKEAVKGGKKK"
     gene            2040..3221
                     /locus_tag="PLB85_01385"
     CDS             2040..3221
                     /locus_tag="PLB85_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72009.1"
                     /translation="MKRYSYLLAILLTALLVLSACSGNKKIQEPVEPPPTPLELAKAA
                     ADTAAICLEEENFALALDYFNQSKEFYLLAQPEATPVDSVDVNIERIQSNIAFTYQNL
                     AHESDQLHQYDDAIMEFESAANIYKSLVPLTMTAAERDVMVAGLYRNMAVTAQKAGQY
                     ERALGYYDNVLQIEPGNEDVLMSKYSILKNDIGDPVRAYQVLKDYAEASNDVNAYLVL
                     ASAYRDEGDNNTAAIYYDKAMEIGQNTMVYSTVADFYRGIKNYKKSNEVLLKLIEATT
                     DNASNALAYRIMADNYDKLNNTAKKIEYYDKSLNLEPNADVALALANHWNKQKNWAKV
                     ITYATKVINIDSSRAAAYLLRGNAYFMQKKNDAAKADLQRIQNDPNYGSSASAILKKI
                     K"
     gene            3288..3737
                     /gene="umuD"
                     /locus_tag="PLB85_01390"
     CDS             3288..3737
                     /gene="umuD"
                     /locus_tag="PLB85_01390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOU72010.1"
                     /translation="MPKKIKSDGSVKALYGVSTETKIKRPLIGNKFPAGFPSPAADYL
                     EGLLDLNEYLIKNKAATFFARVEGDSMIGCGIFPGDIVIVDRSLEAGNSSVVLARYDG
                     EFTIKKLRIEKGELFLVSENESYPPIKINEELEFVIWGVVTTVIHKV"
     gene            3741..6992
                     /locus_tag="PLB85_01395"
     CDS             3741..6992
                     /locus_tag="PLB85_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72011.1"
                     /translation="MRPFILAAVLILSSFAPLAAQGLVLDISLPLLPDQLSYQLAQGP
                     IVSGSESVFADTLLLVPGLDYRLDHRAGALILQRFPEAEYLRVSCLLVPPDLASPRQL
                     YHELDPSDSLFQSVAPRPRSWLPDDGKLLISGAKTFAITFSDEEAFDLKQSLFVNLNG
                     ELSRNVNIAAQLSDSQSKLTPEGDSKELSSLDKVFIRVFGKQYEIAMGDLDWEFTDTR
                     YINYRTSIEGLNAWYRDRHFAQAGFTAAAGKPASQAITIIDGKQGPYYLNPTGWQSSY
                     LITAGSEQIYRNGILLERGTDYYIDYSEGSVMFRNLVVSSDQINAWFQYADEHYKQST
                     LFNSSKIQVLPGLTISHHFIRQADAKDSPLLYQFSPADLDSLSAAGDRVAWGNGITLV
                     EPGLGSYILCYTEEGIPYYEYSAGDTTAVYNLTFSFVGLGNGDYEEYSSGKYRYVGPG
                     MGSWLPQKRLVPAVLRNNADLALNYGYKGLELGVEGIYTTNDKNTFSALDDEDNRSGL
                     LTAWGRLRSGEDASSSWVGLDFERRWDKSFLFSQQSGYAEEYDLSLLAPADSLAQWRL
                     DLTLGSGAWEAWKPQLTLRYRDIPGLYSQKALRLLSKSTGKGVFPALDLRSTLSWQDI
                     VSDGGSSLMQYHDLAGSWDFNWFKAKLLANYNSLEHSQITASEADSRYYRVNPQITLG
                     VAKTSLSQLSFTQDNNFRKDTDWVSSASSQTYALKHSTTTLNHNLNLEFTHRQIQKEG
                     PKTSYNLVSFRNSNYFLQQAVMLLCNYQLNQTEFFPRIRELEYIGSGLGLYDSTGVYT
                     PDGDWDYVYITSGRGTLSSEVNGQVSLYLKPGNQFPKWNWLRGDLILQGTLQDSLMAD
                     WRSYLIFPETALSGPSTIFSNQRLSQTLWLDLVPNRVIGSLGAQFNRSLDNRYQSQSR
                     ASESLYSAEFDLKNLAGNNYNIRYHHSNETDSRYLSDITQNELRMLVQRNFTSNNIGT
                     LNLSGRLENGIQQQSGEGYRLCGLGLEPGYRSVWGKKARVNGSFGLRYNFREGSEFLA
                     FLPEKRGGLLLNWSASAVYRLNSFSSATLEYSGTAYPEQDVKHSLKLEFKAEL"
     gene            7008..7790
                     /locus_tag="PLB85_01400"
     CDS             7008..7790
                     /locus_tag="PLB85_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOU72012.1"
                     /translation="MTEIIALTPVDYILVLPFIFLAGLIDSIAGGGGLISLPAYVAAG
                     LPMHAALATNKFSSACGTIFSTARYLQAKMIDLPVALVSAVLALFGSYLGTRAVLLLS
                     GDFLRWLLLVVIPIIAVFTLIRKNFGHQDNSSQFKLGLRLFLGGLAGLLIGFWDGFFG
                     PGTGTFLILVYALLLRYDFVVANGNTKVVNLASNLAALTAFLIAGKVYFPSAVPAAVF
                     GIAGNMIGSKLVVRNGNCLIKPIFILALALLLGRIVWDLFSR"
     gene            7870..7957
                     /locus_tag="PLB85_01405"
     tRNA            7870..7957
                     /locus_tag="PLB85_01405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7904..7906,aa:Leu,seq:cag)
BASE COUNT         1858 a         2360 c         2063 g         1926 t
ORIGIN      
        1 caatggtatt attaatgtga agttcgcttt tgcaatcaag aatcgttcca attcctgtac
       61 ttaaaatgtc gcgaatcgcc ccattctgca tagccgatgc tttcgccgat ggagaggatg
      121 ctcttttcca gcgcgctgcg cgaactctgg gcattttcat ccaaaccggg ttttccctca
      181 tagagttgat aaccatcccg gaaacttgtg tccgtgatat tgggcatgat gatgttcgcg
      241 ccggccagca ggccaagttc cctgccgctg gacatcaggg cctgcagcgc tgttgtggag
      301 gcgatgttga cgtccctaag ggcaattctg gtggcggcga tcatcttcaa gcccgcctcc
      361 agcgcttttt cggggtcgta agaagggatc aggtgcgcca aaggcgtctc gtgatgcggg
      421 atgaagggtc ccatgccgat catgtcgata tcctcatcat aaaagaaaag gatgtcatca
      481 gccaggtgtt ccgccgtctg gcccggcagc ccgatcatca ctcccgtgcc ggtttggtaa
      541 cccactttcc tcaggctccg caggcattcc aggcgttttt gaaaggaatg tccggcagga
      601 tgcagtgcct tgtataattc ggggttggat tcctctatcc tcaaaaggta tctatgggca
      661 cccgcctcaa accactggcg gtaaactttc tccgtttgct cacccaggga aagtgtgata
      721 cccagttcgc cccggctgag gtttttgatg tccaccacca gatcggtgat aaagtccgac
      781 caggccttgt cttcgcgctc accggcctgt atcaccagcg agccgtagcg ctgttcccag
      841 caccagcgtg cctcggcaag gacttcctcc cggctgatct ggtagcgctt cacgttgggg
      901 ttgccgcagc ggatgccgca gtaaatgcag tttttagtgc agatgttgct taattctatc
      961 agtccccgga aatagacttt ggtgcccaca tattctcttt tcacctcgta ggcgcgttta
     1021 tacagcgctt ccagctccgc cggatcggac agagtgagca tatttatcag ttcgtcgcga
     1081 ttgggtcgca tagggtaaat ccttttccat tcaatcgggg cagccatccg gggcttggac
     1141 tggtttttgc cgccaaatat aacaatataa gcgataagag tgggaaaacc aatctggccc
     1201 cattacatca ggctcatgtt cagtagattg atgcgatgct gtctggccgt taaggccctt
     1261 tcctgtttcc cgtaaacgcc tcccgcataa tgcccgcatt cggtacgagt atagtgcggg
     1321 aggcgtagga gggcgatgga cttgaccgct cagggcgtgg cagacagggc attggccagg
     1381 agcgggaaag caattctgct gggacgaaaa aaggcggaaa ttgatttccg ccttttttag
     1441 gatgtcaatt ctggtttatt tctttttccc acccttgaca gcttctttga gcctcttgcc
     1501 tgctttgaag acaggaactt tacgggctgg gatcttgagg ggtttcttgg tcttggggtt
     1561 gacgccattg cgggctttgc gatgggcaac actgaaggaa ccaaagccta caagggagac
     1621 tttctcctct ttcttgagag cttgagtaac accctctaac attgcgttga gtgcgatgcc
     1681 ggctgttttc tgcgaaattc cggcggtccc tgcgatcttt ttgattaaat catctctgct
     1741 catgttacct ccttgggttt gtatttaaag agtaagagat aatacaaagc gattaaaaac
     1801 tgggtctgga aaatctgtca acaaaaaaat gctgtttttt cagcacttta agggattggg
     1861 gtaacgcaaa tacgttatcg cgatttacca acttattgtc attcaacgag aagcgttttt
     1921 tccacaaggg ataaaaaatt ctgaaaataa tgccctactt cctgcctatt agctttttac
     1981 attgacaaaa aacgccgctc ctctcactgt cgtttaaaca gtaattcact ggaggaataa
     2041 tgaaaaggta ttcatacctg cttgcgattc tgctaacagc tcttctggtg ctgtcagcct
     2101 gcagcggaaa caaaaagatc caagaaccgg tggagccgcc gccaacgccg ctggaactgg
     2161 ccaaagccgc ggcggatact gccgccatct gtttggaaga ggagaatttc gccctggcgc
     2221 tggattattt caaccagtcc aaggaatttt acctgcttgc ccagccagaa gccacgcctg
     2281 tggattcggt ggacgtgaac attgaacgga tccagtccaa tatcgccttc acctatcaga
     2341 atctggccca tgaaagcgac cagctccacc aatatgatga cgccatcatg gagtttgaaa
     2401 gcgctgccaa tatctacaaa agcctggttc cgctaaccat gaccgccgcc gagcgtgacg
     2461 tcatggtggc aggcctctac cgcaacatgg ccgtcaccgc ccagaaagcc gggcaatacg
     2521 aacgcgcact gggttattat gacaacgtgc tccaaatcga gcccggcaac gaagatgtgc
     2581 tgatgtccaa gtacagcatc cttaaaaacg acattggcga cccggttcgc gcctatcagg
     2641 ttctgaaaga ttacgccgag gcctccaatg acgtgaacgc ctatctggtg ctggcctccg
     2701 cctaccgcga cgagggtgac aacaacaccg cggccatcta ttacgacaag gcgatggaaa
     2761 tcggccaaaa cacgatggtg tattccaccg tggcagattt ctaccgcggg atcaagaact
     2821 acaagaaatc caacgaggtg ctgctcaagc tgatcgaagc caccacagac aacgcttcca
     2881 acgcccttgc ctaccgcatc atggctgaca actatgacaa gctgaacaat accgccaaga
     2941 agatcgaata ctatgacaaa tccctgaacc tggaacccaa cgccgatgtg gctctggccc
     3001 tcgccaatca ctggaacaag cagaagaact gggcgaaggt gataacctat gccaccaaag
     3061 tgataaacat cgattcttcc agggccgcgg cctatctcct gcgcggcaac gcctacttca
     3121 tgcaaaagaa aaacgacgcc gccaaagctg acctgcaaag gatccaaaac gatccgaact
     3181 atggctccag cgcgtccgcc attctcaaga agatcaaata agcaatccat attcggcagt
     3241 caaccgtgcg cgatcacggt tgactgttgt tttctgaggt ctccgctatg cctaaaaaga
     3301 tcaagtcaga cggatcggtg aaagcgctct acggggtcag caccgaaacc aagatcaaaa
     3361 ggccgttgat cggcaacaag ttcccggccg gatttccttc tcccgctgcc gattaccttg
     3421 aaggcttgct agacctcaac gaatacctga tcaagaacaa agccgccacc ttcttcgccc
     3481 gggtggaagg cgattcgatg attggctgcg gcatctttcc cggcgatatc gtgatcgtgg
     3541 acaggtcttt ggaagccggc aacagctcgg tggtgctggc ccgctatgac ggggaattca
     3601 cgatcaagaa gctgcgcatc gaaaagggcg aactttttct ggtttcagaa aacgagtcct
     3661 atccgcccat caaaatcaac gaggaacttg agttcgtgat ctggggcgtt gtcacaaccg
     3721 tcattcacaa agtgtagcgt gtgcgaccct ttatcctggc cgcggttctg atcctgagct
     3781 cgtttgcgcc gctggctgcg cagggcttgg tgctggatat cagcttgccc ctgttgcccg
     3841 accagctttc ctaccaactt gctcaggggc ctatcgtttc tgggtcagaa tccgttttcg
     3901 ctgacaccct gctcttggtt ccgggcctcg attaccggct cgaccaccgc gccggagccc
     3961 ttatcctgca gcgctttccc gaagctgaat acctgcgcgt tagttgtctg ctggtgcctc
     4021 ccgacctggc cagcccgcgc cagctttacc acgaactgga cccatccgac agcctgttcc
     4081 agagcgttgc gccccgtccc cgaagctggc ttccggacga cggcaaactg ctcatctccg
     4141 gcgccaagac ctttgccatc accttttcgg acgaggaagc cttcgacctg aagcaatcgc
     4201 tgttcgtgaa cctgaacggc gagctttccc gcaatgtgaa tatcgccgcc cagctttccg
     4261 acagccagtc caaactcact ccggaaggtg attccaagga actcagcagc ctggacaagg
     4321 tctttatccg ggtgttcggc aaacagtatg aaattgctat gggcgacctg gactgggaat
     4381 tcaccgatac ccgctacatc aactaccgca ccagcattga aggcctgaat gcctggtacc
     4441 gggaccgcca tttcgcccag gccggcttca ccgccgccgc ggggaaacca gccagtcagg
     4501 ctatcactat aatcgatggc aaacaggggc cctattacct caatcccacc ggctggcaga
     4561 gctcgtatct gattaccgct ggcagcgagc agatctaccg caacggaatt ctgctggaac
     4621 gaggaacgga ctattatatc gactattccg aaggctcggt gatgtttcgc aacctggtag
     4681 tctccagtga ccagataaat gcctggttcc aatacgcgga cgagcattac aaacaatcca
     4741 ctctcttcaa ttcctccaaa atccaggttc tgccgggcct caccatttcg catcatttca
     4801 tccgccaggc cgatgccaaa gattcccctc tgctctatca gttcagcccg gctgacctcg
     4861 attccctgag tgccgcaggc gaccgcgtgg cttggggaaa cggcatcacg cttgtggagc
     4921 ccggcttggg aagctacatt ctgtgttaca cggaggaggg aattccctac tatgaatact
     4981 ccgcgggcga taccaccgcc gtttacaacc tcactttcag cttcgtgggc ctgggcaacg
     5041 gcgattacga ggaatattcc agcggaaaat accgctacgt gggtcccgga atgggctcct
     5101 ggctgcctca gaaacgtttg gttcccgctg tgctgcgcaa caacgctgac ctggccctga
     5161 actatggcta caagggcctc gagcttggtg tggaaggcat ctacaccacc aatgacaaaa
     5221 acaccttttc cgccttggat gatgaggaca accgcagcgg cttgttgacc gcctggggga
     5281 ggctcagaag tggggaagat gccagctcaa gctgggtagg tcttgatttt gagcggcgct
     5341 gggataagag ctttctcttc agccagcaat ccggttatgc tgaggaatat gacctctcgc
     5401 tgctggcacc ggccgattcg ctggcccagt ggcggcttga cctcactttg ggttcgggcg
     5461 cctgggaggc ctggaagcca caactgacct tgcgttaccg ggatattccg ggcctttaca
     5521 gccagaaagc gttgcgcctg ctctccaaaa gcaccggcaa aggcgtgttt cccgcgctgg
     5581 accttcgttc taccctttcc tggcaagaca ttgtatcgga cgggggttcc agtctgatgc
     5641 agtatcacga tttagccgga agctgggatt tcaactggtt caaagccaaa ctgctggcca
     5701 actacaacag cctcgagcac tcccaaatca cagcttccga agcggacagc cgctactacc
     5761 gtgtgaatcc ccaaatcacc cttggcgtcg ccaaaaccag tctttcacag cttagtttta
     5821 cccaggacaa caacttccgc aaagacacag attgggtcag ctccgccagc tcgcagacct
     5881 acgccctcaa acacagcacc actaccctca accacaatct gaacctggaa ttcacccatc
     5941 gccagatcca aaaggaaggt cccaaaacca gctacaacct cgtttccttt cgcaattcca
     6001 actacttcct gcaacaggct gtgatgctgc tgtgcaatta ccaactcaac caaaccgagt
     6061 tcttcccccg catccgcgaa ttggaataca tcggctccgg actcggcctc tatgacagca
     6121 ctggagttta caccccggat ggcgattggg actacgttta catcaccagt ggacgcggga
     6181 cgctgagttc ggaggtgaac ggtcaggtaa gcctttacct caaacccggc aatcagttcc
     6241 caaaatggaa ctggctgcgt ggagatctca tcctccaggg caccctacag gattcgctga
     6301 tggcggattg gcgcagctac ctcatattcc cggaaaccgc gttgagcggg ccttccacca
     6361 ttttcagcaa ccagcgcctc tcccaaaccc tctggctgga cctggttccc aaccgcgtga
     6421 tcggcagcct tggcgcccag ttcaatcgca gcctcgacaa ccgctaccag agccagtccc
     6481 gagcttccga atcactctac tcagcagagt ttgacctgaa aaacttagcc ggcaacaact
     6541 ataacatccg ctatcaccat agcaacgaaa ccgactcccg ctatctatcc gacattaccc
     6601 agaacgagct cagaatgctc gtgcaacgca actttacttc aaacaacatt ggcaccctga
     6661 acctcagcgg caggctggaa aacggaatac agcagcaaag cggggagggc taccgcctgt
     6721 gcgggctggg cctggaaccc ggctaccgca gtgtgtgggg aaaaaaagcc cgcgtgaacg
     6781 gctcttttgg gctccgctac aatttccgcg agggcagcga gttcctcgct ttcctgcccg
     6841 agaagcgggg tggattgctg ctcaactggt ctgcctccgc ggtttaccgc ctcaacagct
     6901 tcagctccgc gaccctggaa tacagcggca cagcctatcc ggagcaggac gtgaagcatt
     6961 ctctcaaact ggaattcaag gcagaactct gagccgggaa cctgtttgtg actgaaataa
     7021 tcgctctcac cccagttgat tacatactcg tattgccttt catctttctg gctgggctca
     7081 tcgattccat cgccggcgga ggaggcctga tctcgcttcc ggcctacgtc gcggctggtt
     7141 tgccgatgca cgcggcctta gccacgaaca agttttcctc cgcctgcggc accatattca
     7201 gcacagcgcg ctacctccaa gccaagatga tcgacctccc tgtggctctg gttagcgcgg
     7261 ttctggcgct gtttggctcc tatctgggca cccgcgcggt gcttctgctt tccggcgatt
     7321 tcctgcgctg gctattgctg gtggtgattc ccataatcgc agtgttcacc ctcatccgca
     7381 agaacttcgg ccaccaggac aattccagcc agttcaaact cggccttcgg ctgttcctgg
     7441 gcggactggc cggcctcctg ataggcttct gggacggttt ttttggaccg gggaccggta
     7501 ccttccttat tctggtgtac gcgctgctgc tgcgttacga ttttgtggtg gccaatggca
     7561 ataccaaagt tgtaaatcta gcctcgaacc ttgcagcgct taccgccttt ctgattgccg
     7621 ggaaggtgta tttccccagc gctgttcccg ccgcggtgtt tggcattgcc ggaaacatga
     7681 ttggctccaa actggtggtc cgcaacggca actgcctcat caaaccgatt ttcatccttg
     7741 ctttggctct gttgctgggc cgcatcgtct gggacctttt cagccgctga tatccggctt
     7801 ttaggcttcc cgccaaataa aaccttgaca gcaagccccc tttccgggaa atggctccca
     7861 caagcaagtg ctgaagtggt gaaattggta tacacgctag tttcagggac tagtgagcgg
     7921 tgagctcatg ggggttcgag tccccccttc agcaccattt ttttgccctc gtttttcttt
     7981 ctccctttct tcgttcaggt attcggattt cataaggatt cacggttcga actgattgtc
     8041 tttctgtaga gttagcgact ctttctgtgc ttttctctgc tggatcaata cctaacactg
     8101 attttttctg agagggaatc ccgttctgtc gggtaataat tatgtggaca tcctgggcag
     8161 ggtttgataa attgcgaaca agatatcgaa aaaaggaaaa cgacgat
//
LOCUS       DAOOWR010000027         8140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_8802_length_8140_cov_3.447248, whole genome shotgun sequence.
ACCESSION   DAOOWR010000027 DAOOWR010000000
VERSION     DAOOWR010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8802_length_8140_cov_3.447248"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLB85_01410"
     CDS             <1..431
                     /locus_tag="PLB85_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU72013.1"
                     /translation="LSGTGMLGDLLSDLVKEKLKIKRVRSDTFGYLQRSFVGCVSDVD
                     QREAREVGERAAHYAIWYNLDGSISIQRTGFYSVNYQLEKLADVARKTRHMPDEYINS
                     SGNGVTDEFKYYLRPLLGSGFGVAHRLRAPRVANLLKGNA"
     gene            489..1826
                     /gene="gdhA"
                     /locus_tag="PLB85_01415"
     CDS             489..1826
                     /gene="gdhA"
                     /locus_tag="PLB85_01415"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU72014.1"
                     /translation="MTFQEFMNKVTAKNPDQPEFLQAVKEVVETIWDVYQANPRFVKA
                     NIMERLVEPERTIIFRVPWMDDNGDVHVQRGYRVQFNSAIGPYKGGIRFHPSVNLSIM
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFDARGRSDAEIMRFCQSFMAELYRHIGPDTDV
                     PAGDIGVGKREIGYLYGYYRKLVNEFTGVFTGKGSTWGGSLIRPEATGFGVVYFAEEM
                     LKTKGKSFEGLRVALSGFGNVAWGAAQKINELGGKVVTLSGPDGYIYDEDGVSGEKVD
                     YMLELRASNNDRVIDYAKVFPKAKFFEGKRPWEVPVDVAIPCATQNELNVEDAKNLMK
                     NNCFCVTEAANMPCTPEAMEVFLNAKILYAPGKAANAGGVSTSGLEMTQNSMRLSWNR
                     EEVDARLHGIMKNIHEACRENGEQADGYINYVKGANVAGFLKVANAMYDQGII"
     gene            complement(2130..4025)
                     /locus_tag="PLB85_01420"
     CDS             complement(2130..4025)
                     /locus_tag="PLB85_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOU72015.1"
                     /translation="MKKTFLIILGLLGLLAFLPAVPRNLVVVEVGTGTWCGYCPGAAM
                     GCHDLLQNNYPVAIIKNHNGDSYANTYSNARNSFYGITGYPTALFDGQNASVGGSSSS
                     SMYSNYLPKVNARMAVPSHYTISAQGAQVGGDFQIAVTISKPEADTNTNVKLHGVLTE
                     SNIPQVWFNQTTVDNVNRLMAPNQNGTTIDLDTGGTTTVNLTFTPAANWNLANCEVVF
                     FLQNMTSKEILQGVKYSLAGLVGAYPISTDNIAFPDMYVNGSAVIPISIINYISTPAS
                     GTISIDNPAFTSDVSTFNIPGTSSITANITFTPTAAQTYNGTLTITGNLYNHPNITIP
                     LSGTGFINAAPTATDVIISGPPVINQELTGSYSFNDADGNTEGNSILKWYRIVNNTPQ
                     AIAGSNEQAYIATAEDLGFPLAFEVTPVDQHGMPGTAVMSSYTIPIENLPAPRNLQAT
                     VTPPNTVVLTWERPEHFEGKGFVGYRLFRNGLSISSITNPNTLTFTDSGVPSGTHEYW
                     VCTLFNNPMMLSDPSNVVTVNVGVGIDDQIAPAEITVSVHPNPFQTNTAISIQSKADA
                     DVDFAIYNVRGQLVKSFALRTDNSGIANTSWDGTDNQGKPINSGVYYYRMNTTGQLRN
                     GRIVLMK"
     gene            complement(4133..4774)
                     /locus_tag="PLB85_01425"
     CDS             complement(4133..4774)
                     /locus_tag="PLB85_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU72016.1"
                     /translation="MRVKPTLICDFDGTVADSIAMILELINQLAPRYGYRTISPELFE
                     QVRDLPLPKACRAVKFPLHKLGHAIAVVLHEYRKMIPDLEPCPDVVPMLEKLKGQGIS
                     LGLISSNHTENLLAWLEHNQIGCFDWVEGTSGILRKHRSIRARIRAHNLDRERVVYLG
                     DEVRDIKAARRNRLKIVSVSWGLHSKSNLLKHNPDRVVNEPHELMEIIPELIS"
     gene            complement(4765..5604)
                     /gene="rsmI"
                     /locus_tag="PLB85_01430"
     CDS             complement(4765..5604)
                     /gene="rsmI"
                     /locus_tag="PLB85_01430"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU72017.1"
                     /translation="MPAGSLYLVPVPVGNLGDITLRALDTLKSVDLIAAEDTRKTRFL
                     LSHYEIKAPRLLSLHKYNEKQRLAEILALLHEGKSIAVVSDAGSPGISDPAFWLVRAA
                     IEAGVDIIPLPGATALIPAITASGLDTGSFLFLGFLPLKQKDRAAKLGLVASSPHTVI
                     IYEAPHRLKQTLGDILARCGDRRVCLAREISKLHEEFIRGSLEGILADYQMTEKGEFV
                     VLIEAAAPEQPASEEEIKSCIAELAPGPESSKELAENIAKRFKVSRNRAYQMILDIRK
                     KCG"
     gene            complement(5614..6801)
                     /locus_tag="PLB85_01435"
     CDS             complement(5614..6801)
                     /locus_tag="PLB85_01435"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOU72018.1"
                     /translation="MFGKMKTDLQNLFEELKKQGLYKNERIITTPQGANIGVSTGESV
                     MNFCANNYLGLGNHPEVVKAAQDIMNDWGYGLASVRFICGTQQIHKDLERKISEFLGT
                     EDTILYVACFDANAGVFEPLLDDESAIISDELNHASIIDGVRLCKAKRFRYKHSNMKE
                     LEKCLIEAKDMKYRLICTDGVFSMDGDMAKLPEICDLAEKYDALVMVDDSHATGYIGK
                     KGRGTPEHFGVQHRVDIVTSTLGKAMGGANGGFTSGRKEIIEVLRQRSRPYLFSNSVA
                     PSVVGASIKVIELLSESSELRDKVWENARYFRSEMIKAGFDIVEGNTAIVPVMLYDEP
                     LAMKMADMLLKEGIYVIGFVYPVVPKGKARIRVQLSAAHSREDLDKAVAAFVKVGKEL
                     KLV"
     gene            complement(6899..6987)
                     /locus_tag="PLB85_01440"
     tRNA            complement(6899..6987)
                     /locus_tag="PLB85_01440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6950..6952),aa:Leu,seq:aag)
     assembly_gap    7021..7030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7031..7401)
                     /gene="secG"
                     /locus_tag="PLB85_01445"
     CDS             complement(<7031..7401)
                     /gene="secG"
                     /locus_tag="PLB85_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU72019.1"
                     /translation="MLIYTIALVIHIIISVALVLVILSQTSKGGLDANLGGAAMNVFG
                     GSGASKFLRKWTQILGVIFVVSCLFLAFQVSKVNETKAPKVLSETFGDTKAPAKQSAP
                     APTPGQTAPAGSATQQTPPAET"
     gene            complement(7444..>8140)
                     /locus_tag="PLB85_01450"
     CDS             complement(7444..>8140)
                     /locus_tag="PLB85_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU72020.1"
                     /translation="MLFLASSFQQFGMLWTGAGSAGFITGLYVVFVPLIGLGRGQKLN
                     PPLILAVALSLAGLALINGKPDLQATLGNSLVLIGAVFWALHVQLIDKLTRFHSSLRL
                     AAFQYWVCAFLSLVGSIVLQMVSKQSFVATDFLNSIWSASLPLLYAGIMSVGVAFTLQ
                     LHAQKKVQPHVASVILCMEGVFAMLGGWLLLRERVSATATLGAALLLVAMLVSVWQER
                     REFLIDKKAACKT"
BASE COUNT         1785 a         2097 c         2368 g         1880 t
ORIGIN      
        1 agctttccgg aacgggtatg ctgggcgatc ttctgagcga cctggtgaag gaaaaactca
       61 aaatcaagcg cgtgcgttct gacacttttg gctatctgca gcgttctttc gtaggctgcg
      121 tttcagatgt ggaccagcgc gaagcccgcg aagtgggtga acgtgccgcc cattacgcga
      181 tctggtacaa tctggatggc tcaatttcca tccagcgcac ggggttttac tcagtgaact
      241 accagctcga aaagctggct gacgtggccc gcaagacccg ccacatgcct gatgaataca
      301 tcaattccag cggcaacggg gtcaccgatg agttcaaata ttacctccgg ccgctgcttg
      361 gttcaggctt cggcgtagcg cataggctcc gtgcccccag ggtggcaaac ttgctcaagg
      421 gaaacgcttg acattaagtc gcgcgataaa ataatggtca aaaaacaaat acagatatgg
      481 aggaacccat gacctttcaa gaattcatga acaaagtcac cgccaagaac ccggatcaac
      541 ccgaatttct gcaggcggta aaagaagtcg tcgaaaccat ttgggacgtc tatcaggcca
      601 atccccgctt tgtgaaagcc aacattatgg aacgcttggt cgagcctgaa cgcacgatca
      661 ttttccgcgt tccctggatg gatgacaatg gtgatgtgca tgtgcagcgc ggctaccgcg
      721 tgcagttcaa cagcgccatc ggcccctaca agggcggcat ccgcttccac cccagcgtga
      781 acctctccat tatgaagttc ctcggctttg agcaaatttt caaaaacagc ctcaccaccc
      841 tgcccatggg tggcggcaaa ggcggttcgg attttgacgc cagaggaaga tcagacgctg
      901 agatcatgcg tttctgccaa tccttcatgg ctgaactcta ccgccatatc ggcccggaca
      961 cagacgttcc cgctggcgac atcggcgtgg gcaagcgcga aatcggctat ctttatggct
     1021 attatagaaa gctggtcaat gagttcaccg gcgttttcac cggcaaaggc agtacctggg
     1081 gcggaagcct aatccgtcct gaagccaccg gctttggcgt ggtctatttc gccgaagaaa
     1141 tgctcaagac caagggcaag agcttcgaag gcctgcgcgt tgcgttgtcc ggatttggca
     1201 acgttgcctg gggcgctgcc cagaagatca acgagctggg aggcaaagtg gtcaccctct
     1261 ccggtcccga cggctacatc tatgatgaag acggcgtttc cggcgaaaag gttgactaca
     1321 tgctcgagct ccgcgcttcg aacaatgacc gcgttatcga ttatgccaag gtcttcccca
     1381 aagccaaatt cttcgaaggc aagcgtcctt gggaagttcc cgtggatgtg gccatcccct
     1441 gcgccactca gaacgaactg aacgtggaag acgccaaaaa cctcatgaag aacaactgtt
     1501 tctgcgtcac cgaagccgcg aacatgccat gcacacctga agcgatggaa gtgttcttga
     1561 acgccaagat cctctacgcg cctggcaaag cagccaacgc cggcggtgtc tccaccagcg
     1621 gactggaaat gacccaaaac tccatgcgcc tgagctggaa ccgcgaagaa gtcgacgctc
     1681 gcctgcacgg aatcatgaag aacatccatg aagcttgccg cgaaaacggc gaacaggccg
     1741 atggttacat caactatgtg aaaggcgcca acgtcgccgg cttcctgaaa gtcgccaacg
     1801 ccatgtacga ccagggcatc atctaaacaa caaacaacca tccaacatat cacccggctc
     1861 acccgagccg ggtttttttt acctttcctg attgactcca cctttgccct ttccaatcca
     1921 catcccattc acttcccgca gacttcccgc ttgccaaacg ggaattctac gggaagatgt
     1981 caggatgcga gtgggatggg gcaaagcacg gaaacaggga cgatcaccct ggagagcgga
     2041 ctggcaaagg aaaagaagaa ccccggacct tgtgatccgg ggctgggtgc ttattatgtg
     2101 gactggtatg aaaccgtcct cggatggttt tatttcatca ggacgatgcg tccgttcctg
     2161 agctgtcctg tggtgttcat ccggtagtaa tagacgccgc tgttgatcgg cttgccctgg
     2221 ttgtcagtgc cgtcccaact ggtgttagcg atgccggaat tgtcagtgcg gagggcgaag
     2281 ctcttcacaa gctggccgcg gacgttgtag atggcaaaat ccacgtcagc atcagctttg
     2341 ctttggatgc tgatggctgt attggtctgg aagggattgg gatgcacgct cacggtgatc
     2401 tccgcgggcg cgatctggtc gtcaataccc acgccgacgt tgacagtgac aacattggaa
     2461 ggatcggaga gcatcatggg gttgttgaac agggtgcaga cccagtattc gtgggttccg
     2521 gagggaactc cgctgtctgt gaaggtgagg gtgttcgggt tcgtgatgct ggaaatgctg
     2581 aggccgttgc ggaagaggcg gtagcccaca aatccctttc cttcaaagtg ttccgggcgt
     2641 tcccaggtga ggaccacggt gtttgggggt gtaacggtgg cttgcagatt ccggggcgcg
     2701 ggcaagttct cgatggggat ggtgtaagaa ctcattaccg ctgtgccggg catgccgtgc
     2761 tgatcaacgg gggtcacctc gaaagcgagg gggaagccca gatcttctgc tgtggcgata
     2821 taagcttgct cattggagcc ggctatagcc tggggtgtgt tgttcacgat cctgtaccac
     2881 ttcaggatcg aattgccttc ggtgtttccg tccgcatcat tgaaggaata ggaccctgtc
     2941 agctcctgat tgataacggg cgggccgctg attatcacat cggtggcagt gggggcggcg
     3001 ttgataaagc ccgttccgga gaggggaatg gtgatgttgg gatggttgta gaggttgccg
     3061 gtgatggtca gcgttccgtt gtaggtctgg gcggcagtag gggtgaaagt gatgttggcc
     3121 gtgatggatg aggttcccgg aatgttgaaa gtggatacat cgctggtaaa ggcgggattg
     3181 tcaatggaga tggtcccgct ggcaggagtt gagatgtagt ttattatcga gatgggaata
     3241 accgcgctgc cgttcacata catgtctgga aaagcaatgt tgtcggtgga gatgggatac
     3301 gcgcccacca gtccggccag agagtatttc acgccctgga ggatttcctt ggaggtcatg
     3361 ttctggagga agaagaccac ttcacaattg gccaggttcc agtttgcggc aggggtgaaa
     3421 gtcaggttga cggttgtggt cccacctgtg tccaggtcga tggtggttcc gttctgattg
     3481 ggggccatta agcggttgac gttgtccact gtggtttggt tgaaccagac ttgggggatg
     3541 ttcgattcgg tgagaacgcc atgcagcttg acgttggtgt tggtgtccgc ctccggtttg
     3601 ctgatggtca cggcaatttg gaaatcgccg cccacctggg caccctgggc actgatggtg
     3661 tagtgagaag gcacagccat acgcgcgttc acctttggca gatagttcga atacattgaa
     3721 ctggagctgc ttccgcccac ggaggcattc tggccgtcaa acaacgcggt ggggtaaccg
     3781 gtgattccat aaaaactgtt gcgggcgttg gaataggtgt tggcgtatga gtcgccgttg
     3841 tggtttttaa taattgccac ggggtagttg ttctgcaaca ggtcgtgaca tcccattgcc
     3901 gcgcctgggc aataaccgca ccaagtgccg gtgcctactt caaccaccac aagattgcgc
     3961 ggaaccgctg gcaggaaagc cagcagaccg agtaagccga gtatgatcaa aaaggtcttt
     4021 ttcattttga gctccttgat attatgttat tagtaatgtt tcatatctgt tacgcctatg
     4081 gtgaatccct gtattttgtc aagaaaatcc tgctgagatc gccaaggctc tgtcaggata
     4141 taagttccgg aataatctcc atcagttcat ggggttcatt gaccactcgg tcggggttgt
     4201 gtttgagcag attggattta gaatgcaggc cccaactaac gctcacgatt ttcagccgat
     4261 tacgccgcgc ggctttgatg tcgcggactt cgtcgcccag atacacaacg cgctcacggt
     4321 ccagattgtg agccctgatc cgggctctga tgctgcggtg tttgcgcagg atgccactgg
     4381 tgccttccac ccagtcgaaa cagcctattt gattatgctc gagccatgcc agcaggtttt
     4441 cggtatggtt ggaactgatc aggccaaggg aaattccctg tcccttaagc ttttcgagca
     4501 taggaaccac gtcggggcag ggctccaggt cgggaatcat tttgcggtat tcgtgcaaca
     4561 caaccgcgat ggcatggccg agtttgtgga gggggaattt cactgcccgg caggctttgg
     4621 gcaggggcag gtcacgaact tgttcaaaca gctctgggct gatggtgcgg tagccgtagc
     4681 gaggggcgag ttgatttatc agttccagga tcatggcgat ggaatcggcc acggtgccgt
     4741 caaaatcgca aatcagggtc ggtttcaccc gcatttcttc ctaatgtcca ggatcatttg
     4801 gtatgctcgg ttgcggctga ccttgaagcg tttggcgata ttttccgcga gttccttgct
     4861 gctctcagga ccgggcgcga gttccgcgat gcagcttttg atttcctctt ccgaggcagg
     4921 ctgctccggc gcggcggctt caataaggac tacaaattca cccttttccg tcatctgata
     4981 atcggccaaa atgccttcca ggctgccgcg gatgaactct tcgtgcagct tgctgatttc
     5041 gcgggcgagg cagacccgcc tgtccccgca gcgggcaagg atgtctccca aggtctgctt
     5101 gagcctgtgc ggagcttcgt aaataatgac ggtgtgggga ctggaagcga caagaccgag
     5161 tttggcagcg cggtctttct gcttgagggg caggaagccc agaaagagaa aactgccggt
     5221 atccaggccg gaagctgtaa tggcgggaat aagggctgtg gcccctggca ggggtataat
     5281 gtccactcca gcctcgatgg cggcgcggac caaccaaaaa gccgggtcgg aaatccccgg
     5341 gctgccggcg tcggaaacga ccgcgatgga ctttccttca tgcagcaggg cgaggatttc
     5401 cgccaggcgt tgtttttcgt tgtatttgtg caggcttaga agcctgggcg ctttgatctc
     5461 gtaatgggag agcaggaagc gggttttgcg ggtgtcttcg gcagcgatca ggtccacgct
     5521 tttcaaggtg tccagcgcgc ggagggtgat gtcacccagg tttcccactg gcaccgggac
     5581 cagatacagg ctgcctgctg gcaaatccgc ctcttaaacc agtttaagtt ctttgcctac
     5641 cttgacgaag gcggcgacgg ccttatcgag gtcctcccgg ctgtgcgcgg ccgagagctg
     5701 gacgcggatg cgggctttgc ctttcggcac cacgggatag acgaagccga tcacgtaaat
     5761 gccttctttc agtagcatgt cggccatctt catcgccaga ggctcgtcgt aaagcatcac
     5821 gggaacgatg gcggtgttgc cttccacgat gtcgaagccg gctttgatca tttctgagcg
     5881 gaagtagcgg gcgttttccc aaaccttgtc acgcagttcc gaagattcgg aaagcaattc
     5941 gatcaccttg atgctggcac cgaccacgga cggtgcaacg ctgttcgaga acaggtaggg
     6001 acggctgcgc tggcgcagaa cttcgattat ttctttgcgg ccggaggtga aaccgccgtt
     6061 ggcaccgccc atcgctttac caagggtgct ggtcacgatg tccacgcggt gctgaacgcc
     6121 aaaatgctcc ggggttccgc ggcctttctt gccgatgtag ccggtggcgt gggaatcgtc
     6181 caccatcacc agtgcgtcgt atttttcggc caggtcgcaa atttcaggca atttggccat
     6241 atcgccgtcc atggagaaaa ctccgtcggt gcagatcagg cggtacttca tgtccttagc
     6301 ttcgatgagg catttttcca gttctttcat gttcgagtgt ttgtagcgga agcgcttggc
     6361 cttgcaaagc ctcacgccgt cgataatgga agcgtggttc agttcgtcgg agataatggc
     6421 gctttcatcg tccagaaggg gttcgaacac tcctgcgttg gcgtcgaagc aggcgacgta
     6481 gaggatggtg tcctcggtgc caagaaactc ggaaatcttg cgttccaagt ccttatggat
     6541 ttgctgcgtg ccacagatga agcgcacgga ggcgaggccg taaccccagt cgttcatgat
     6601 gtcctgtgcg gctttcacca cctcgggatg gttgcccagg ccaagatagt tgttcgcgca
     6661 gaagttcatt acgctttcgc ctgtgctcac gccgatattg gctccctggg gtgtggtgat
     6721 gattcgttcg tttttgtaca atccctgttt tttcagttcc tcaaacaggt tttgcaggtc
     6781 agttttcatt ttgccaaaca tggcaaacct ccatattatg attgtttgtt taattccaca
     6841 cttttggagg cttcgattca gtcaataaaa aaagctcccg gtgaggggag ccagattttg
     6901 gtgcagaagg cgggactcga acccgcacac ccgttacagg cacaagatcc ttagtcttgc
     6961 gtgtctgcca aattccacca cttctgcatc tgtaaaaggt tcgggtcaat tgcccggagt
     7021 nnnnnnnnnn gtttccgcgg ggggcgtctg ctgcgtcgcc gaaccggctg gagcggtctg
     7081 gcctggcgtg ggcgcgggag cgctttgctt ggccggagct ttggtgtcgc cgaatgtttc
     7141 gctgaggact ttgggggcct tggtctcgtt caccttggaa acctggaaag ccaggaacag
     7201 gcaggaaacc acaaaaatca cacccaggat ctgggtccat ttgcgcagaa acttggaggc
     7261 gccgctgccg ccgaacacat tcatcgcggc tccgccgagg ttggcgtcca ggccaccctt
     7321 ggaggtttgc gatagaatca ccagcaccag cgcgacgctg attatgatat ggatcaccaa
     7381 ggcaatcgtg tatataagca tgtttctttc cttatgtaac ttcgaaaaag cattgttcgg
     7441 aaactaagtt ttgcaagcgg cttttttgtc aatcagaaat tcccgccgtt cttgccaaac
     7501 gcttaccaac attgccacca aaagcagcgc cgcgccaagc gtggcggtgg ctgaaaccct
     7561 ttctcgcaaa agcagccagc ctcccaacat ggcgaaaacc ccttccatac agaggatcac
     7621 gctggcgaca tggggctgga cttttttctg ggcgtgcaat tgcagggtga aagccactcc
     7681 cacagacatt attccagcgt agagcagagg caggctagca gaccagattg agttcagaaa
     7741 gtccgtggca acgaagcttt gcttgctgac catttggaga acaatgcttc caaccaggct
     7801 caggaacgcg caaacccagt attggaaagc ggcaaggcgc aaactggagt ggaatcgggt
     7861 gagtttgtcg atgagctgca catgcaatgc ccaaaacaca gctccgatca gcaccaggga
     7921 gttgcccaga gtggcttgca ggtcaggctt tccattgata agagccaggc cggctaaact
     7981 caaggccacg gccaaaatca ggggagggtt gagcttttgt ccccgtccca aaccgatcaa
     8041 aggaacaaac accacataga gaccggtgat gaagcccgca ctgccagctc cggtccaaag
     8101 catgccaaac tgctgaaagc tggaggcgag gaacagcatc
//
LOCUS       DAOOWR010000028         7957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9076_length_7957_cov_3.599342, whole genome shotgun sequence.
ACCESSION   DAOOWR010000028 DAOOWR010000000
VERSION     DAOOWR010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9076_length_7957_cov_3.599342"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLB85_01455"
     CDS             complement(<1..255)
                     /locus_tag="PLB85_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU72021.1"
                     /translation="MNYILPFLLALAILSISLGCQAQIPANPVQAVDSVELDRYLGVW
                     YQQSFIPFRWQKADCGELLTAEYSLNPQGKINVVNTCYADK"
     gene            457..2013
                     /locus_tag="PLB85_01460"
     CDS             457..2013
                     /locus_tag="PLB85_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOU72022.1"
                     /translation="MLKLTLDDSVKQRARAAAASIAGSFGWLFDGYTSLAIERTVLRL
                     MGIDGALEDGNPLPNVVVDHLRVGQALDKGAAIPVANAMLGRKLSAQSVADAVSRGEI
                     DLAKQPQAAPDEIFSLLNTLADEMLGHIAKQRATREQMFAKYGPRRVPAIYVIVATGN
                     IYEDAVQAKAAASEGADIIAVIRSTAQSLLDYVPFGATTVGFGGTYATQENFRIMRAA
                     LDEVSEKEQRYISLTNYASGLCMPEIAAMGAIERLDLMLNDAMYGILFRDINPKRTLL
                     DQYFSRMINAFAGIEIQTGEDNYLTTSDAVEKAYTVTASQLLNEQFALLSGVKPEMMG
                     LGHAYEIDPELENSFSYELAHALLTRELFPQAPVKYMPPTKFASGNIFKTHLMDAMFN
                     FAGQLTGQGVQLLGMMTEAIHTPHLADRSLAIENAKYVFKAMKNLNDSLCLSPDSFIN
                     QRANQVLVEAAEFLEGVAAEGLFPAMARGDFADIKRPETGGKGLDGVFRKDKNYFNPF
                     LNKMTKELGI"
     gene            2010..2753
                     /locus_tag="PLB85_01465"
     CDS             2010..2753
                     /locus_tag="PLB85_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOU72023.1"
                     /translation="MSKKTMIRPYGDTSGDGKMQLSFSLPVPPDAFGKEAARLLLKNI
                     GYDEIEICGLRDMGEGYTHFIAYAVTSAFVDPASIKVKVVETPVMDMEATDAYISENI
                     GRVITVVGACIESDAHTVGIDAIMNMKGYNHRYGLERYQWFNAINLGAQVPSEELLRR
                     ARAENADAILVSQVVTQKNIHIKNLTRLIELAEAEQLRDKMLFICGGPRISHDLAIEL
                     GYDAGFSTGTYSTDVASYIAITLAGRIQK"
     gene            2922..4208
                     /gene="rseP"
                     /locus_tag="PLB85_01470"
     CDS             2922..4208
                     /gene="rseP"
                     /locus_tag="PLB85_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOU72024.1"
                     /translation="MTLLYTILAFGIMIFIHELGHYKAARWFGVGIEKFSIGFGKPLK
                     EWKRSGVAWRIGWIPLGGYVKMRGENPDSPDKDAADSFFRLAWWKKALIGFSGPFANL
                     LLGLLLFIFSFVLPVHIQDQYPVVSRAEGSWPQVFAPGDSIVSVNGKSVRGFSEFLEG
                     LLERPRSTVLFSRSGEKRSAEIRAKDVDSLLAGVYPVADTRVGEVVPKTPAYHAKLQT
                     GDCITAVDSVAVDDWYAMRQMIINAPRDQVLLTIERNGESFNKSLKLESSLNTGGSRA
                     IGITQYQPVRYDRRFNPIEAIKLGAYNTANFILMNYQMLGKLLRSPGEIRKSVGGPVM
                     IATMSQQIGERGFGSLLVFFGGISLMLMIMNLLPIPILDGGVILFSIIEGIIRRPIPL
                     KIQSVLQSIGFVLLMGLMILALYSDVASELLRFMNR"
     gene            4531..5640
                     /gene="tgt"
                     /locus_tag="PLB85_01475"
     CDS             4531..5640
                     /gene="tgt"
                     /locus_tag="PLB85_01475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU72025.1"
                     /translation="MFKFKLEKTSGFARAATISTSHGKIKTPVFMPVGTLGTVKAMSP
                     HELEESHAQIILGNTYHLYLRPGHELIRDAGGLHKFISWYKPMLTDSGGFQVMSLAAL
                     RKISKEGVKFQSHIDGSRHFFTPEGVIGIQEALGADIIMSFDECPPYPATREYVEKSL
                     KTTLQWAERGKAAFKNAKKQALFGIVQGGIYEDLRQRSALALMDMDFPGYSIGGLAVG
                     EEKEDMFRITAFLNDILPADKPRYLMGVGTPSDLLLNIANGVDMFDCVMPTRNARKGS
                     IFTRHGKMIIKAARYKEDFSPIDPDCGCYTCQHFSRAYIRHLISMNEILGMRLTTIHS
                     LWFYQELMQMARAAILEDRYSDFLAEMLPVIDRVI"
     gene            complement(6284..6484)
                     /locus_tag="PLB85_01480"
     CDS             complement(6284..6484)
                     /locus_tag="PLB85_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72026.1"
                     /translation="MARWFAKLRDGGFANLFQAEAQALVERIWADNILSIKKLPLGHL
                     TGIVRISCPISSLVLILHEIPD"
     gene            6559..>7957
                     /locus_tag="PLB85_01485"
     CDS             6559..>7957
                     /locus_tag="PLB85_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU72027.1"
                     /translation="MHRLKTLLTVLLVIFGTLGLAAAPRFGERPFIDIYQVPDSAMEQ
                     GHIRIKLSEANSALAQSFQYQDGALASFGLEELDALNLQYGVKKISPVFGEVNKNLKW
                     GWRHVEWGLHLWFELEYDSEADIREIVMAYRNLAKDVQWAEPEYKKTLFSVNQGEVSA
                     IADNLVRWTPNDPRYGEQWHYHNTGQTGGTVDADIDLPEAWDIEKGHSDVVVCIQDQG
                     VQYDHPDLAANMWINPGEIAGNGIDDDNNGYVDDVYGYNFYQSTSNIVAGDHGCHVAG
                     TVAAVTNNGVGVSGVAGGSGTGNGVRLMSTQVFDPTSGGAGFEQAPIYAADNGACISQ
                     NSWGYTNVGSYEQSVLDAIDYFNANGGGSVMSNGITIYAAGNSQSSGQWYPGCYSGVF
                     AVAATTHNDTKAWYSNYDTWVDVSAPGGETNSVTSQGVLSCWSGSNYGFYQGTSMACP
                     HTSGVAALVVSYAHRN"
BASE COUNT         1877 a         2235 c         2110 g         1735 t
ORIGIN      
        1 cttgtcagcg tagcaggtgt tcacaacgtt tatcttgccc tgggggttga gggaatattc
       61 tgcggtcagc aattctccgc aatcggcttt ttgccagcgg aaagggataa atgactgctg
      121 gtaccaaacg cccaggtagc ggtcaagttc cacgctgtca accgcttgca caggatttgc
      181 ggggatttgc gcctggcagc ccaggctgat gctcaaaatc gcgagagcca gcaaaaatgg
      241 gaggatgtaa ttcataatgt atcctttgtt ttatggatag aatacactat ttcgctggaa
      301 aaacaaccga ctctgcattt tgttcaggct tcgtttgggt tttaccggaa cttgggtttg
      361 ttcgcttgga aaaggatttc cgccttttcc cttgacggat aagcagggcc taaaaaaacg
      421 tgaaaaaata taaccaacaa agctttggag agttacatgc taaaactcac tctcgacgat
      481 tccgtcaagc aacgtgcccg agcggctgcc gccagcatcg ccggttcctt tggctggctg
      541 tttgacggct atacctcact ggcgatcgaa cgcacggtgc tgcgcctgat gggcatcgac
      601 ggcgcactgg aagatggaaa cccgcttccc aacgtggttg tggaccatct gcgcgtggga
      661 caggccctgg ataagggagc cgccatcccg gtggccaacg ccatgctggg ccgcaagtta
      721 agcgcccagt cggtcgcgga tgccgtaagc aggggcgaaa tcgaccttgc taaacagccc
      781 caggccgctc cggatgaaat cttcagcttg ctgaataccc ttgcagacga aatgcttggc
      841 cacattgcca aacagcgcgc cacccgggag caaatgtttg ccaaatacgg cccccgcagg
      901 gttccggcta tttatgttat tgtggcgacg ggcaacattt acgaggacgc ggtgcaagcc
      961 aaagccgccg catccgaagg cgctgacatc attgccgtga tccgttccac cgctcaatcg
     1021 ctgctggact acgtgccttt cggcgctaca acggttggct tcgggggaac ttatgccacc
     1081 caggaaaact ttcgcatcat gcgtgccgcc ctggacgagg tatcggaaaa agagcagcgc
     1141 tacatcagcc taacgaacta cgcttcaggg ctctgcatgc ccgagatcgc ggcgatgggc
     1201 gctattgaga ggctcgacct gatgctcaat gacgccatgt acggcatcct tttccgcgac
     1261 atcaacccca agcgcacctt gctggaccag tatttcagcc gcatgatcaa tgcctttgcc
     1321 ggcatcgaga tccagaccgg agaggacaac tatctcacca cctccgacgc cgtggaaaag
     1381 gcctacaccg tcacggcctc gcagcttctg aacgagcagt tcgccctgct tagcggtgtg
     1441 aagcctgaaa tgatgggcct cggccatgct tacgagattg atccggaact ggagaacagc
     1501 ttttcctatg agcttgccca cgctctgctc acccgcgaac tctttcccca ggcgccggtt
     1561 aagtatatgc cgcccaccaa gttcgccagc ggcaacatct tcaagaccca tctcatggac
     1621 gccatgttca acttcgcagg ccagctcacc ggccaggggg tccaactgtt gggcatgatg
     1681 accgaggcga tccacactcc ccatctggcg gacagatccc tggccataga aaacgcgaag
     1741 tatgtcttca aagccatgaa aaacctgaat gacagcctct gcctgagccc ggacagcttt
     1801 atcaaccagc gcgccaacca ggtgctggtt gaagccgcgg agtttctaga gggcgtggcc
     1861 gccgaaggtc ttttccccgc catggccaga ggcgactttg ccgacatcaa acgtcccgaa
     1921 acaggcggca agggcctcga tggcgttttc cgcaaggaca aaaattactt caaccccttc
     1981 ctgaacaaga tgaccaagga gctgggaata tgagcaagaa aaccatgatc cgtccctacg
     2041 gcgacacctc cggcgacggc aagatgcagc tttccttcag tttgcccgtg ccgccggacg
     2101 cctttggcaa agaggcagcg cgcctgcttc tgaaaaacat cggctacgat gagatcgaaa
     2161 tctgcggact gcgcgacatg ggcgaaggct acacccattt tatagcctac gccgttactt
     2221 cggcgtttgt ggatcccgcc agcatcaagg tcaaagtggt ggaaacaccc gtcatggata
     2281 tggaggccac cgacgcctat atcagtgaaa acatcggccg cgtgatcaca gtcgttggcg
     2341 cctgcatcga aagcgacgcc cacactgtgg ggatcgacgc cattatgaac atgaaaggct
     2401 acaaccaccg ctacggcctg gaacgctatc agtggttcaa cgctatcaac ctcggcgccc
     2461 aagtgccttc ggaagagctg ctgcgccgtg ccagggccga aaatgccgat gcgatcctcg
     2521 tttcccaggt agtgacccag aagaacatcc acatcaaaaa cctcacccgg ctcatcgaac
     2581 tggctgaggc tgaacagctt agggacaaaa tgctcttcat ctgcgggggc cccaggattt
     2641 cgcacgatct cgccatcgaa ctgggttatg atgccggttt tagcaccgga acctattcca
     2701 ccgatgtcgc gtcttatatc gcgataacgc tggcgggacg gatccagaag tagcatatgc
     2761 ctggcgcttg atgttgatgc tgagacccag aagctttccc aaatcaatat ctatggcttg
     2821 atatcatgga cgtgatgttg cgtgttatcg acctcctcca ccatcacagt ataatgacat
     2881 cagcttttca atctttcacc ctttaaccct tttcacgaac aatgactttg ctctatacca
     2941 tcctcgcctt tggcatcatg atcttcatcc acgaactggg acattacaaa gccgctcgct
     3001 ggtttggagt tggcatcgaa aaattctcca tcggctttgg caagcccctg aaagagtgga
     3061 agcgcagcgg agttgcctgg cgcataggct ggataccgct gggcggttat gtaaagatgc
     3121 gaggcgaaaa tccagattct ccggacaagg atgccgcgga ttcatttttt cgcctggcct
     3181 ggtggaaaaa ggcgctgatc ggtttcagtg ggccctttgc caatctgttg ctgggtttgc
     3241 tgctgttcat cttttccttt gtcctgcccg tccacattca ggaccagtat cctgtggtga
     3301 gccgcgccga aggttcctgg ccgcaggtat ttgctcccgg agacagcatc gtgagcgtaa
     3361 acggcaaatc tgtgcggggt ttcagcgaat ttcttgaagg tctgctggaa aggccgcgca
     3421 gcaccgtcct ctttagtcgt tcaggtgaaa aaagatctgc ggagataagg gcgaaggatg
     3481 tggattcact tctggcagga gtttatccag tcgccgatac ccgggtgggg gaagtggtgc
     3541 ccaaaacccc ggcctaccat gccaaactgc aaaccggaga ctgcatcacg gcggtggatt
     3601 ctgtggctgt ggacgattgg tacgccatgc ggcagatgat tatcaacgcc ccgcgggacc
     3661 aggttttgct caccatcgaa cggaacgggg aaagcttcaa caaaagcctg aagctggaat
     3721 ccagccttaa taccggtggc agccgcgcca tcggtataac gcaataccag cctgtgcgtt
     3781 acgaccgacg cttcaacccc attgaggcca tcaaactggg cgcttacaac accgccaact
     3841 tcatcttgat gaactatcag atgctgggta aattgctgcg cagccccggg gagattagaa
     3901 aatcggttgg tggaccggtg atgatcgcca ccatgagcca gcagatcgga gagcgcggct
     3961 tcggcagtct gctcgtcttc tttggcggga tcagcctcat gctgatgatc atgaacctgc
     4021 tgcccatccc catcctggat ggaggcgtga tccttttttc cataatcgag ggcataatcc
     4081 gccggccaat ccccctcaag atccagtcag tattgcaatc aataggcttc gttcttttga
     4141 tgggtttaat gattctagct ctgtattctg atgtggccag tgaactgctt aggtttatga
     4201 acagatgagt aaaagggaaa aggagaaaaa gttggcttgg gttcgggggt tgtctttccc
     4261 aaaacggagc tttttgaata ttgccaacgg ctcgttatcc gaatttgaaa cacccagttg
     4321 gaacttgcgt tccggctcga gctttaccaa gattttgaag cttatgcttc ccaaatcaga
     4381 tgtatccgct caatgctgat gaatctgatc aaggcactca aggcgaaaaa atgacaaagt
     4441 cagatagagc tttgttaatt ttgaatgata ggttttggcc tatgcacaac cttttctctt
     4501 tttccctttt tctctttatc cgcctaaacc atgtttaaat tcaaactcga aaaaacctcc
     4561 ggatttgcaa gagccgccac gatttccacc tctcatggca agataaaaac ccccgttttc
     4621 atgcccgttg gcacacttgg caccgtgaag gcgatgagcc cccacgaatt ggaggaaagc
     4681 cacgctcaga tcatcctggg caacacttac cacctctatc tgcgtccggg gcatgaactt
     4741 atccgcgacg ccggcggcct gcacaaattc atttcctggt acaagcccat gctcaccgac
     4801 agcggtggat ttcaggtgat gagcctggct gcgctgcgta aaatcagcaa agagggcgtc
     4861 aaattccagt cccacatcga cggcagccgc cattttttca ctccggaggg cgtcatcggc
     4921 atccaggaag cccttggcgc cgacatcatc atgagtttcg acgaatgccc gccctatcct
     4981 gccacccgcg aatacgtgga aaaatcgctc aaaaccacgc tccagtgggc cgaaaggggc
     5041 aaggcggcct tcaaaaacgc caaaaagcag gccctcttcg gaatcgtgca gggcggcatt
     5101 tatgaagacc tgcgccaacg ctctgccctg gcgctgatgg acatggattt tccgggctac
     5161 tcaattggtg gattggccgt gggcgaggaa aaagaggaca tgttccgcat cacggctttc
     5221 ctgaacgaca tccttccagc agacaaaccc cgctacctga tgggcgtggg cacccccagc
     5281 gacctgctgc tgaacatcgc caacggcgtg gacatgttcg actgcgttat gccgacccgc
     5341 aacgcccgca aaggcagcat cttcacccgc cacggcaaaa tgataatcaa ggccgcccgc
     5401 tacaaagagg atttttcccc catcgatccc gactgcggct gctacacctg ccagcacttc
     5461 tcccgagcct acatccgcca tctgatcagc atgaacgaga ttttgggcat gcgcctcacc
     5521 accatccaca gcctctggtt ttaccaggaa ctcatgcaaa tggcccgcgc ggcaatcctg
     5581 gaggaccgtt actccgattt cctcgctgaa atgctgcccg tcatcgaccg agttatctga
     5641 ctactcttaa cctctcagct aattatcccc gacgcctttc cgaattggat gtgctgcggc
     5701 aagttgaccc aggccaacgt caatccatca attccgttgg aaacggctca tgttcaatag
     5761 gagtgggtgc tactactgcc atcatagctc gtgggctagc ctggcgtaca gcgaacgtag
     5821 tgagcatgga cgacaggatg agtagcgaaa tggacgatgg ataattatgc gttaatggag
     5881 gataggataa tttggattga tttctctttg ctggcgtaga tgctcgttcc tcgctcgacg
     5941 ccagcttgta gcctggggcg gctctgtctg gccgttaagg ccctttactg ggtcccggat
     6001 gcgcctcccg cacaatcccc gtatttggtg cgagaattat acgaggggca tgggaggggt
     6061 atggtatcga gcgctcaggg cctgactggc aggacataac ggtggctaaa catttctggc
     6121 tttctattaa gtcaggctct ctttcagatg gagtgcttgt ccggcacttt caagcccgta
     6181 gggcggcaga acgatagtga gggcgcgtaa gcccctcgtc acgcgaattc ccggtctcca
     6241 gaagagcctc gtagaggcga cagaacaata acgagggtgt gaatcagtca gggatctcgt
     6301 ggaggatcaa aacaaggctt gaaatcgggc aagaaattct cacaatgcca gttaaatgac
     6361 caagtggcaa ctttttgatt gacaaaatat tgtctgccca aattctttcc acaagcgcct
     6421 gagcttcagc ttggaaaaga ttggcaaaac cgccatcacg aagttttgca aaccaacgcg
     6481 ccaagcgctg ccgggaccgc taacagccat gaacagtaat aaatataagc gacaatacat
     6541 tatcacaagg agttgaaaat gcacagattg aaaacactgc tgaccgtttt gcttgtcatc
     6601 tttggcacgc ttggtctggc agccgctccg cgctttggag agcggccttt catcgacatc
     6661 taccaggttc ctgacagcgc catggaacaa ggccacatca gaatcaagct cagcgaggcc
     6721 aattccgcgc tggcgcaaag ctttcaatat caggacggcg ccctcgccag cttcggcctc
     6781 gaggaactgg atgccctcaa ccttcaatac ggggtgaaaa agatcagtcc cgtcttcggt
     6841 gaagtgaaca aaaacctcaa atggggctgg cgccacgttg aatggggcct gcacctctgg
     6901 ttcgagctgg agtatgatag cgaagctgac atccgcgaaa tcgtgatggc ctaccggaac
     6961 ctggccaaag acgtccagtg ggctgaacct gaatacaaga aaaccctgtt cagcgtgaac
     7021 cagggtgaag tttccgccat cgcggataac cttgttcgct ggaccccgaa cgacccgcgc
     7081 tatggtgaac agtggcatta tcacaacacc ggccagaccg gcggaacggt ggatgccgac
     7141 atcgacctgc ctgaagcctg ggacatcgaa aaaggccaca gcgatgtggt ggtctgcatc
     7201 caggaccagg gtgtccagta cgaccatccg gatttggctg ccaatatgtg gatcaatccc
     7261 ggcgaaatcg ccggaaacgg cattgacgac gacaacaacg gctatgtgga tgatgtgtat
     7321 ggctataact tctaccagag cacttccaat atcgtggcgg gagaccacgg ctgccatgtg
     7381 gcgggaaccg tggcggctgt cacaaacaac ggtgtaggtg tctccggcgt tgccggcgga
     7441 tccggaaccg gcaacggcgt gcggctgatg agcacccagg ttttcgatcc cacttccgga
     7501 ggcgctggtt ttgagcaggc tccgatttat gctgccgaca acggcgcctg catctcccag
     7561 aacagttggg gttacaccaa cgtgggaagc tacgagcagt ctgtgcttga cgccatcgac
     7621 tacttcaacg ccaacggcgg cggaagcgtg atgtccaacg gcatcaccat ctacgcagcg
     7681 ggcaacagcc aatccagcgg ccagtggtat cccggttgct acagcggcgt ttttgccgtg
     7741 gcagccacca cccacaacga taccaaggca tggtattcaa actatgacac ctgggtggac
     7801 gtctccgccc ccggcggtga gacaaacagc gtcacctccc agggcgtgct gagctgctgg
     7861 agcggaagca actacggctt ctaccagggc acctcgatgg cctgcccgca tacttccggc
     7921 gtggcggctc tggttgtttc ctatgcccac cgcaatg
//
LOCUS       DAOOWR010000029         7854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9224_length_7854_cov_3.506603, whole genome shotgun sequence.
ACCESSION   DAOOWR010000029 DAOOWR010000000
VERSION     DAOOWR010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9224_length_7854_cov_3.506603"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="PLB85_01490"
     CDS             complement(<1..1668)
                     /locus_tag="PLB85_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOU72028.1"
                     /translation="MKKPLLFTLLLIFVIGMFSNVYAQVNVTIGTGTSTNTETGVPAP
                     YGTWYRNFRQQFLLLATEIEDAGGGAGNINSVAFNVANLNTCSPMPNYTIRLKHTTQT
                     SLSTTFEVGDYQTVYTQAEFMPVVGWNTHTFSTPFVWDGTSNILVDICTTLVTGNYTR
                     NASVYYSSTTFNSALRYQSDNSDAGSATTGTTGTNRSNVRFNMESVVVTDPPNPAQIV
                     SPLDGAVNVMTSATLNWASGGGVPSGYKVHFGTTNPPPFIQNTTETDYAPTNMELTTT
                     YYWQIIPFNVMGDATNCPVWSFTTGGPVVLMSNGTQNVPDGMTYSFYDSGGPDGNYQS
                     SENYTFTFTATNPSSIIHVLFTEFHTESGWDFLKVYNGPDTSAPQIGPAAGYTGTTLP
                     EEIIGSNAVTFVFTSDSSYTYSGWSAAVSAVDLQHDLGAQSISGNTTPSVNSPSTYTV
                     AVKNNGANTENTYTVKLMGTGDVELASIPGTTIAPQQTIDFGLTWTPTAPGAMQIWGK
                     VVLADDGIATNNQTLPLNVTVQQEGIVAVTIGDGSETARIPVDFFWKN"
     gene            complement(1957..2670)
                     /locus_tag="PLB85_01495"
     CDS             complement(1957..2670)
                     /locus_tag="PLB85_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOU72029.1"
                     /translation="MEEQLRTLAKMQKLDDQIGALRLLQQQLPKELNDIIERVDQSAA
                     NLLNSQAELAETEKKQRALEGDIKQNQSTIQKYTTQLTEIKTNKEYKALNSEIAYLKD
                     KISELESQQLELMDEENAAKQKVEQDKQDLEAAEKTKRDREGDLRKKIESLDAEIDKL
                     RAERTLLAKTLPQNLVRQYANMIKNKGNCAVVYARNGACGGCGYVIRPQIRIELQLRN
                     KVNFCESCGRILMEEFKDL"
     gene            complement(2673..3494)
                     /gene="nadC"
                     /locus_tag="PLB85_01500"
     CDS             complement(2673..3494)
                     /gene="nadC"
                     /locus_tag="PLB85_01500"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOU72030.1"
                     /translation="MNSSEIIRLALAEDIGSGDISTAYLELEPVPEKAFMIAKAKGVL
                     AGLDIAREVFLTVDKSLKITLYSRDGDEVTPGSEIMRVEGNPASILQGERTALNFLQR
                     MSGIATLTRSMVRELEGTSVRLLDTRKTTPLLRGLEKYAVRVGGGFNHRFGLYDMVML
                     KENHIRACGGITEAVNRVKKHNTAHKIEVEVTNIQELEEAVSAGVDRVMLDNMNLTQI
                     RACVRRFGKKVELEVSGGVTLANIAKLAQTGIHYISSGALTHSYQSLDISLLFKE"
     gene            3740..4762
                     /locus_tag="PLB85_01505"
     CDS             3740..4762
                     /locus_tag="PLB85_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU72031.1"
                     /translation="MLQRPYHRLGLLLIALLASACAGTRGNVYPLDEAYLRSERMFAR
                     LQEIMDLNPALVQLNIIGFSSSENLPIYCLDIGRPHATSNVLLIGQHHGDEVLGLEVV
                     LDWAEKLASKSREDKKIDAILDVYRFWIVPTLNPEGHRVVSAGLSSSKRKNNRDTNAN
                     GKLDLHEDGVDLNRNYPVFWEAFDSPPPHHQNYKGPEPASEKEILAIIQLAQKQSFDY
                     AIFYHSSPSGALSEKIFLPALDRENARQVKAYEELEDFARAYARLLPKDYLRGRYEVS
                     PAPGTRMGTARNYFFHMHGTKAFLVEIGGVNTSGISIIHPGKAIRDKIVAKHLKALTK
                     VFYAML"
     gene            complement(5034..7157)
                     /locus_tag="PLB85_01510"
     CDS             complement(5034..7157)
                     /locus_tag="PLB85_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72032.1"
                     /translation="MSKSFVFALACLWLIAACLGAEADVPKALPESFSRPVELMSTPS
                     PEKASSDQKTTPSWIFSKHPTSIIHSYYDYMIGSFNNLPLNLIPSSAGGGYFLTWTGR
                     GTPGGECMPYYGYLDYDGNMVNAARIETQSSGAHGMPATVVDPVSGKPFYAWTQFVDS
                     DPEAEIVLASDAFIAGIAGLFNPPQIVIDNPITITAPDGTVTTDNVFFWPSLVIGPSP
                     VTNARRLYLLGRQNCNVYLAMADFTADDIEMETPLSWQYTSVPELNAWALEAEPRRTH
                     LALEADPAGNVILAGYHDSDEVENDIDIFVCGFFGEGDWTRHSFSSRISTWNPGDYFT
                     DDEGVPYTDDELHWELMNSGHLNATLDRSGRLHIVGLWGLANADGGFYSLLQFVKEAV
                     FNPGGGNFVINDIFPKKSPLDIFNACFTPWDREYPFGEVDEFIYDPVNESYYPLISQD
                     WPFPHWDQTAHEGSMMYYYNNIKVTRANPQGQLAVVWQDSNRARSWCAYQTPGYEAWE
                     HTPEIYIAYSGCDGYYWFDPIVLNNVEIPEMVNIKPMWVYPADQMLFTGYSGGGKMGK
                     LGLMFYNDFTWGANVLDPPYHPDPDGGEVMFMELVIIDDSDADDPGQMPAPAILAQNW
                     PNPFNPRTSISFELRQPGQARLEIFDLRGRLVNTLLDKELGTGTYIEDWDGTDANGAP
                     VASGVYFYRLSAGGTSVSRKMLLLK"
     gene            7542..>7854
                     /locus_tag="PLB85_01515"
     CDS             7542..>7854
                     /locus_tag="PLB85_01515"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU72033.1"
                     /translation="MPTDLEIARKIKLQPIDAIAAGIGLAPEDLEHYGEYKAKVRYSA
                     LERIKDNKPGKLILVSAITPTPAGEGKTTVAIGLSQALNLSGQSSIVAIREPSLGPVF
                     GI"
BASE COUNT         1683 a         2068 c         2219 g         1884 t
ORIGIN      
        1 gtttttccag aaaaagtcaa ccggaatgcg agcagtctcg ctaccgtcac cgatagtaac
       61 agccacaatc ccttcctgct gcacggtcac gtttagaggc agggtttggt tgttggtggc
      121 tatgccatcg tcggcaagca ccactttacc ccaaatctgc atcgcgccgg gggctgtggg
      181 agtccaggta aggccaaagt cgatggtctg ctggggagcg atggtggtgc cgggaatgga
      241 tgctagttcc acatctccgg tgcccatcag cttcacagtg taggtgtttt cggtgttggc
      301 gccgttgttt ttcaccgcca ccgtataggt gctgggactg ttcaccgagg gagtggtgtt
      361 accggaaatg ctctgcgcgc caagatcgtg ttggaggtcc accgcgctga ccgcggctga
      421 ccatccgcta tacgtgtaag agctgtcgga agtgaatacg aaagtcacag cgttggagcc
      481 gatgatctct tcaggcagag tggtgcctgt gtagccggca gcgggtccga tctgcggggc
      541 ggaggtatcc ggtccgttat agaccttcag gaaatcccag ccgctttccg tatggaattc
      601 cgtgaacaac acgtggatga tcgaggaggg attggtagcc gtgaaggtga aggtgtagtt
      661 ctcagagctt tgatagttgc cgtcgggacc gccggaatca tagaaagaat aggtcattcc
      721 atccggcaca ttctgagtgc cgttggacat gaggactacc ggtccacccg tggtgaagct
      781 ccatacgggg cagtttgtcg cgtcacccat gacgttgaag ggaattatct gccagtagta
      841 ggtggtggtg agttccatgt tggtgggcgc atagtcggtt tcagtggtgt tttggatgaa
      901 ggggggtggg ttggttgtac cgaaatgcac tttgtagcct gagggcacgc cgcctccgga
      961 agcccagttg agggtggcgg aggtcatcac gttcacagcg ccgtcaagcg gcgagacgat
     1021 ctgcgcaggg ttgggcggat cggtcaccac aacggattcc atgttgaacc tgacgtttga
     1081 acggttggtg ccggtggttc cagtggtggc ggaaccggca tcgctgttat cgctttggta
     1141 gcgcagggca ctattgaaag tggtggagga gtaataaaca gaagcattac gggtgtagtt
     1201 cccagtaacg agagtggtgc agatatcgac aagaatgttc gaagtgccgt cccagacgaa
     1261 gggagtcgag aaggtgtggg tattccagcc aaccacgggc atgaactctg cctgcgtgta
     1321 cacggtctga tagtccccaa cctcaaatgt ggtgctaagg ctggtctggg tggtgtgttt
     1381 cagacgaatt gtgtagttcg gcatcgggga acaggtgttc agattggcca cattgaaggc
     1441 cactgaattg atgttgcccg ctccaccgcc cgcgtcctcg atttccgtgg ccagtagcag
     1501 aaactgctgg cggaaattcc tgtaccaagt tccatagggg gccggaacac cggtctcggt
     1561 gttggttgag gtcccagtac caatggtaac gttgacttgg gcatagacat tgctgaacat
     1621 gccgatcaca aaaatcagca gcagcgtgaa aagaaggggt tttttcatag gatatctcct
     1681 tttcccattt ttatattctc gggttaatac tcaaagtgcg ccggacggca gagccctgaa
     1741 agaaaaggat gccccgtccg tgcctgatat tgacatttat gtatgtatat catcggttct
     1801 caatcgaacc tcccaaattt tctaacactc tatgaacgcc caccttcgga gcttaaaaaa
     1861 tctaatcctg tcattctgct tttctgtcaa gcaataaatt gccaatccca ttaaagaatt
     1921 gctggccaaa caggttacgg cctgcattag agggggttag aggtccttaa attcctccat
     1981 caggatgcgc ccgcagcttt cgcagaaatt caccttgttg cgcagctgca gctcgatgcg
     2041 gatctgggga cgaatcacat aaccgcaacc gccgcaggca ccgttgcgag catagaccac
     2101 ggcgcagttg cccttgttct ttatcatgtt ggcatattgg cgcacgaggt tttggggcag
     2161 ggtcttggcc agcagggtgc gttcagcgcg gagcttgtcg atctcggcat caagggattc
     2221 aattttcttg cgcaggtcgc cctcgcggtc gcgtttggtc ttttccgcgg cttcgaggtc
     2281 ttgcttgtcc tgctccacct tctgtttggc ggcattctcc tcatccatca attcgagttg
     2341 ctggctttcc agttccgaaa tcttgtcttt cagataggcg atctcgctgt tcagggcctt
     2401 gtattccttg ttggtcttga tctcggtgag ttgggtggtg tatttttgga tggtgctctg
     2461 gttttgtttg atgtcgcctt ccaaggcgcg ttgtttcttt tctgtctcgg caagttcggc
     2521 ctggctgttc agcaggttcg cggcggactg gtccacgcgt tcgatgatat cgttaagttc
     2581 tttgggcagt tgctgctgca gcagcctcag ggcaccgatc tggtcgtcca gtttttgcat
     2641 tttggccagg gtgcgcagtt gttcttccat cgtcactcct tgaataaaag actgatgtcc
     2701 agcgactggt aggaatgcgt caaagcgccg ctggagatat aatgtatgcc cgtctgggca
     2761 agtttggcaa tgttggcaag ggtaactccg cccgatactt ccagctccac cttttttccg
     2821 aagcggcgca cgcaggcgcg gatctgggtg aggttcatgt tgtccagcat caccctgtcc
     2881 accccggcgc tgaccgcctc ttcgagttcc tggatgttgg ttacctccac ctcgatcttg
     2941 tgggcggtat tgtgtttttt cacccggttg acagcttcgg tgatgcctcc gcaggcgcgg
     3001 atgtggtttt cctttagcat caccatgtca tagaggccga agcggtgatt gaagccacct
     3061 cccaccctca cggcatattt ttccagcccg cgcaaaagtg gtgtggtttt gcgggtgtca
     3121 agcaaacgca cagaagtgcc ctcaagctcg cgaaccatgg agcgggtaag ggtggcaatg
     3181 ccgctcatcc tctggagaaa attcagcgcg gtgcgctcgc cctgaaggat gctggcggga
     3241 tttccctcca cgcgcatgat ctcggagcct ggcgtcacct cgtcgccgtc ccggctgtac
     3301 agcgtgatct tgaggctttt gtccaccgtg aggaaaacct cccgggcgat atcgagcccg
     3361 gccagcacac ctttggcttt ggcgatcatg aaggcctttt caggaacggg ctccagttcc
     3421 agataggcag tgctgatatc tccgctgccg atgtcctcgg ccaaggccag gcgaatgatt
     3481 tcactactgt tcatgctgcc tccgggataa aaaaatagca tctgggcgtg cagatgctat
     3541 tttattgaaa ttgttgatag tcagatcaca tatctctttg tatcgggtac acatacgatt
     3601 ttaaaacctc ttcttaggtt cattcaatct caaggctcca tcttgtcaag aagtatgttg
     3661 aaaagatgta cgttgcgcgc ttgctgcgcc ggcgccgcaa atatgttgac ggaaagatgc
     3721 gcttgcgtaa agtggtttta tgttacaaag accctatcat cgcctgggcc tgctgctgat
     3781 cgccctgctg gcctcagcct gtgccggaac gcggggaaac gtttatcccc tggatgaggc
     3841 ttatctgcgt tccgaaagga tgttcgcgcg ccttcaggag atcatggacc tcaatcccgc
     3901 ccttgtgcag ctgaacatca tcggcttcag cagcagtgaa aacctgccta tctactgtct
     3961 ggacattggc cgtccccacg ccacaagcaa cgtcctgctg atcggccagc atcacggcga
     4021 tgaggtgctg ggcctggaag tcgtgctgga ctgggcggaa aaactggcct ccaaaagccg
     4081 tgaggataaa aagatagacg ccatcctcga tgtttaccgc ttctggatcg tgccaaccct
     4141 caatcctgaa gggcaccgcg tggtctcagc cggcctcagc tccagcaaac gcaaaaacaa
     4201 ccgcgacacc aacgccaacg gcaaactgga cctccacgaa gacggagttg acctcaaccg
     4261 caattacccg gttttctggg aagctttcga ctccccgcct ccccaccacc agaactataa
     4321 ggggcctgag cccgcctcgg aaaaggaaat cctggccatt atccaactgg cccagaaaca
     4381 gagcttcgac tacgcgattt tctaccacag ctcaccctcc ggcgccctca gcgaaaagat
     4441 attcctgccc gccctcgacc gggaaaatgc caggcaagtg aaagcctatg aggaacttga
     4501 ggatttcgcc cgtgcctatg ccagactgct tcccaaggat tacctgcgcg gcagatacga
     4561 ggtcagtcct gccccgggaa cccgcatggg aacggcccgc aactactttt tccacatgca
     4621 cggcacaaag gcctttctgg tggagatagg cggcgtaaac acttcaggga tcagtataat
     4681 ccatccaggc aaagcaatcc gcgacaagat cgtggccaaa cacctaaagg cgcttaccaa
     4741 ggttttttac gccatgcttt gaaaccatgt agcctggcgt ctagcgaact tagtgagcat
     4801 gggcggcagg atattttgga gttattattc tttgctggca tcaatgcccg ttactctctc
     4861 gacgccagcg tattgcctgg cgatgccgac cggccgttaa ggccctgacc tgtgttccag
     4921 gtgtgcctcc cgcataattc ccgcacttga tgcgagtatt atacgggagg cttgagagag
     4981 ggatggatta gggcgctcag gacgtgaccg gcaggacctt agggattggg ctgtcacttc
     5041 agcaatagca ttttgcggct gacgctcgtt cctcctgcgc tgaggcggta aaaatagact
     5101 ccgctggcca ccggggcacc gttcgcgtcg gttccgtccc agtcctcgat gtatgtccct
     5161 gttcccaact ctttgtccag cagggtgttc accagtcttc cgcggaggtc gaagatttcc
     5221 agccgggcct ggccgggctg ccgcagctcg aaactgatgc tggttctggg attgaaggga
     5281 ttgggccaat tctgcgccag aatcgccgga gcgggcatct ggccgggatc gtcggcgtcc
     5341 gaatcatcaa tgatcaccag ctccataaac attacctcgc caccgtcggg atcaggatgg
     5401 tagggcgggt ccaaaacatt cgcgccccag gtgaaatcgt tgtagaacat caagcccagt
     5461 ttccccattt ttcctccacc actatagccc gtgaacagca tctggtcagc gggatagacc
     5521 cacatcggct tgatattcac catttcggga atctccacgt tgttgagcac gatcgggtcg
     5581 aaccaatagt agccatcaca gcccgaatag gctatataga tttcaggtgt gtgttcccaa
     5641 gcctcatagc ccggggtttg gtaagcgcac catgaacggg cgcggtttga atcctgccag
     5701 accacagcca actggccctg ggggttggcg cgggtgacct tgatgttgtt gtagtagtac
     5761 atcatgctgc cttcatgggc ggtctggtcc cagtggggga aaggccagtc ctggctgatg
     5821 aggggataat agctttcgtt caccggatca tagatgaatt catccacctc gccgaaagga
     5881 tattccctgt cccagggtgt gaaacaggca ttgaagatat cgaggggact ttttttggga
     5941 aagatgtcgt tgataacaaa attgccgcca ccagggttga aaactgcttc cttcacgaac
     6001 tggaggagag agtaaaaccc gccatccgcg ttggccaggc cccaaagacc cacgatgtgc
     6061 agcctgccgc ttctgtccag ggtggcgttc agatggccgg agttcatcag ttcccagtga
     6121 agttcgtcat ccgtgtaggg aacgccttcg tcatcggtga aatagtcgcc tggattccag
     6181 gtggaaatcc ggctgctgaa gctgtggcgc gtccagtctc cctccccaaa aaagccgcag
     6241 acgaagatgt cgatatcatt ctcaacctca tcgctgtcgt gatatccggc caggatgaca
     6301 ttgccggcgg gatccgcctc cagggccaga tgggtcctgc gcggctcggc ttccagcgcc
     6361 caggcgttga gttccggaac gctggtgtac tgccagctta ggggggtctc catttcgata
     6421 tcatcggccg tgaaatccgc catggccaga tagacgttac agttctgccg tcccagcaga
     6481 taaagcctgc gcgcattcgt caccggggag gggccgatga ccaggctggg ccagaagaaa
     6541 acgttgtctg tggtcacagt cccgtccgga gcggtgatgg tgatggggtt gtcgatgacg
     6601 atctggggag ggttgaacaa accggcaatg ccggctatga aagcatctga ggcgagcacg
     6661 atctcggctt ccgggtccga gtccacaaac tgggtccagg cgtagaaagg ctttccgcta
     6721 acggggtcca ccaccgttgc cggcatgccg tgcgcgccgc tgctctgggt ttcgatccgg
     6781 gcagcgttga ccatgtttcc atcgtagtcg agatagccgt aatagggcat gcattcgccg
     6841 ccgggtgtgc cacgccctgt ccaggtgagg aaataaccgc cgccagcgct ggaggggatg
     6901 aggttaaggg gcaggttgtt gaagctgccg atcatgtagt cgtagtagct atgtatgatc
     6961 gaggtgggat gtttgctaaa gatccaggat ggcgtggtct tttggtctga cgaagctttt
     7021 tccggtgaag gcgttgacat cagctccacc gggcgcgaaa atgattcggg aagggccttc
     7081 ggcacatccg cttccgcccc caggcatgca gcaatcagcc acagacaggc cagcgcgaaa
     7141 acaaagctct tgctcatctt tttagctctt ttactctttc tgccctcacg tgcaagaggc
     7201 tgtggtatca aatacttgtc aagttttatt ttgcctcaca tatccctgtc ttttggacag
     7261 agtattttca accggttctt tctgcccata tccatattac atcgactgat gattcgtcgc
     7321 tctccagact atccgaaaca tggtcaactt gtagtagata aagcaaaata gctgtctcca
     7381 aagtatggct ttgcagagaa gaaactgatt tttattgttc aggagacata aagatttcgg
     7441 agaattgtgc cagatcggga aattcaaacg gtcggtcaag agggtttgcc tgtcccggaa
     7501 acgtggctaa cttatctaaa aaacagggaa ggagacacag aatgcccact gatctagaaa
     7561 tcgcaagaaa gataaagttg cagccgattg atgccatcgc ggctggaatc ggcctggcgc
     7621 cagaggattt ggaacattac ggtgagtaca aggccaaggt gcgctattcc gctttggaga
     7681 ggatcaaaga caataagccc gggaaactta tcctggtttc ggccatcacc cccacccccg
     7741 cgggagaagg gaaaaccacc gttgccatcg gtctttccca ggcgctgaac ctgagcggcc
     7801 aaagctcgat agtggccatc cgtgaacctt ctctgggacc ggttttcggc atca
//
LOCUS       DAOOWR010000030         7819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9283_length_7819_cov_3.775631, whole genome shotgun sequence.
ACCESSION   DAOOWR010000030 DAOOWR010000000
VERSION     DAOOWR010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9283_length_7819_cov_3.775631"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PLB85_01520"
     CDS             <1..1546
                     /locus_tag="PLB85_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72034.1"
                     /translation="VVGSQCGSGMYIFQLTDILNWQQMSFIEGGYDSNGVICNGYLVF
                     GDYETDMSRLYVYDISNLSQPLLVSSDPYPPGLEEYPAMERLLACGDFLFHSCTFYGC
                     LCLKVTDSGTVVFAGECYDLPMYSSFGCKYGNYILQPVCFRGIACFDISQPESPQYAF
                     SVFPDRTVYALVNGNYLLAWMTPAGSYGTLTTEAIYDISDLQNPQLVWSQPYSLQSRI
                     FFNPREPEHFYCLAADGLSVSKYQISGGQASLLFSHPNPVVLRDPVFIRGILYQASVS
                     PSSPDRYDLYATNGFEQNTPQAPVVREGFIPGLWAMLDAGNYLYIRDYPGPTCIFHNE
                     LSTFEVASDIRAFAFHDYVCVGRPDGVSFYDTTVPLTGFQTEDLFLPQYSYSVNIAWD
                     DEYLYLFGQDNIAIYAYTLTTGANGEELPKPPEIQLSASPNPFSGSVEISFELKSPAQ
                     AELAVYNLRGQKLRDLSSASYGPGKHSLTWDGKDHSGTPVPAGIYLLRLRAEGLETAF
                     KKISKY"
     gene            complement(1991..3622)
                     /gene="groL"
                     /locus_tag="PLB85_01525"
     CDS             complement(1991..3622)
                     /gene="groL"
                     /locus_tag="PLB85_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU72035.1"
                     /translation="MAKQMLYSHEARTSLKKGVDQLADAVKVTLGPRGRNVVLDKKYG
                     SPTITNDGVTIAKEIELEGEFENMGAQLCKEVAEKTHDNAGDGTTTATLLAQAIIEEG
                     LKHVTAGVNPMYLKRGLEKATKVVVDKIHEYSKEIKSNEEIAQIASISANNDPEIGKL
                     IAEAMESVGKEGIINIEEAKSIDTGLEKVEGMQFDRGYLSPYFVTNADKMIAELEDPF
                     ILVHDKKISVLKDLLPILQEVSQQGRPMLIIAEDIEGEALATLVVNKLRGVLNVVAVK
                     APGFGDRRKAMLEDIAVLTGATVISEEMGRRLDSATMTDLGRAKKIIVEKENTTIREG
                     AGSDEAVSARVKQIKAQIEETTSDYDREKLQERLAKLSSGVAVIRIGAATETEMKEKK
                     ARVDDALHATRAAVEEGIVPGGGVTLIQAAKALKTMKDLDSEEKMAVEILMKALERPA
                     YQIAANAGEEGAVVVEKLKGYKEIHMGYNAANGKFEDLIKAGIIDPAKVVRSAVQNAA
                     SIAALMLTTECIITDIKEPEPPAPMPNPGMGGGMY"
     gene            3791..4735
                     /locus_tag="PLB85_01530"
     CDS             3791..4735
                     /locus_tag="PLB85_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOU72036.1"
                     /translation="MDKHIESALAQLLPELARHGISVSSQRELQYGLQLRLERDAETA
                     TLNLYWSLKKGISQVLAGKPNRLKADLAKLLNAAEAGPVGDFHTWERWIGSDECGKGD
                     YFGALVVAAFALDAEKVDEVRSLGVLDSKKLTDIQVRDIAHNLYSRFPERIACIVIKP
                     LRYNEIMADRQSRGENLNDLLAWQHGAAIMELLGRIPLVQGVLVDQFSLHKKVSKYLK
                     NKEIGIPVEERPKAEADPAVAAASIIARYQFLQSRQAMDRHYKMSFPLGSGKNVLPPA
                     KAFADKYGWDRLAEVAKLHFVTSRGVRVKDIFDPPADK"
     gene            4892..5716
                     /locus_tag="PLB85_01535"
     CDS             4892..5716
                     /locus_tag="PLB85_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU72037.1"
                     /translation="MIKKMGRLNLDDYRIMDAHVDKIFKNLDKGNNGIAERQIFDMAN
                     TQNYFVREELGKRLAVYEGKGKLERICGDMLEHNLYGIRATALFYFYYRHQSEPAIIA
                     QTLDKTCETVPWESETICFEMWKKAPEVMQEYMPRWSTSENPKKREMSLHGMENIASR
                     SPQYVLTFISRLLDDEDEEVQKKISHILVQVGRQRPLQTYASIRRWLLEGDETRARTI
                     WATLKKLTSIFSQKNMRDKAQEFVSISQRVVKGWKSDSDPAVVQLGHKLGKLIKDI"
     gene            5713..6456
                     /locus_tag="PLB85_01540"
     CDS             5713..6456
                     /locus_tag="PLB85_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU72038.1"
                     /translation="MIHVILVEPIYEGNVGAVARIMHNFGFTGLRIVGAVPSKNDFYL
                     AMHSEHLLENALVFPTLAEAVTDLDRVIAFSRRLGKLKPADLNPPQMADYCTKIPQLE
                     IGLVFGRETFGLTDAEAALCPLRCHIPANPAFPSINLAQAVTIALWELSRGGEKSARI
                     KAVSGIELDKMKAYMLDVMEATGFFRSHETTNWEMFIAKMLAQLNPTKTMLWRFRQMF
                     NRWHVLVTGRGRGYERSLPAGQDTEDKPE"
     gene            6520..7332
                     /locus_tag="PLB85_01545"
     CDS             6520..7332
                     /locus_tag="PLB85_01545"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU72039.1"
                     /translation="MTLLTARTELDPRQQEIFKFCEAYFFAHENPALVNKNARYFTEG
                     YDAFGIKEDELRELRDQLLERFEPSVPELAELAYHLFATGKYEFGSLALMLLKKHRPR
                     FDRSVFDQLKRTLDDVVENWAHADVIGTKLTPVLLELNLVDLADFEPWRFSPSKWTRR
                     VAAVTLLYLRSHRPVEQLLEFIQPLMQDNTRPVQQGVGWFLRELWKVHSREVEDFLFA
                     NKDTAPRLVIQYATEKMNKDKKKRFRRAISKAVKPNKHQSRKDKPPQEDSGE"
     gene            7325..>7819
                     /locus_tag="PLB85_01550"
     CDS             7325..>7819
                     /locus_tag="PLB85_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72040.1"
                     /translation="MNKLFPIIFLLCLPLLLPAQQLSVSLDLSALTPGTDELPAGWGV
                     LTAPGFPRLPVKTVNIVLTPEAANLTFSYQFTGLKSVPSPPPFLNPPFTDGECVIAGP
                     ASLSGAARVVYKGIGHWGEVAYASFSVLPATHTGAEWQAWQQLRLDLSWEQPAETASK
                     TLPPV"
BASE COUNT         1733 a         2244 c         2063 g         1779 t
ORIGIN      
        1 cgtggttggc agccaatgcg gctcggggat gtacatcttc cagctcacgg atatcctgaa
       61 ctggcagcag atgtcgttca tcgagggcgg ttatgactcc aacggcgtga tctgcaatgg
      121 ctatctggtg ttcggagact atgaaacaga catgtcccgg ctgtatgtct atgacatatc
      181 gaaccttagc cagcccctgc tggtctccag cgacccctac ccgccaggcc tggaagaata
      241 tcccgccatg gagaggttgc tggcctgcgg cgactttctc tttcacagtt gcacctttta
      301 tggctgcctc tgcttgaagg tgacagactc cggcaccgtg gtctttgccg gtgaatgtta
      361 cgacctgccc atgtatagta gcttcggctg taaatacggc aactacatcc tgcagccggt
      421 ctgtttccgc ggcatagcct gctttgacat ctcccagccc gaatctccac aatacgcctt
      481 ctccgtgttt cccgacagga cggtttacgc gctggtgaac ggcaattacc tgctggcctg
      541 gatgacccct gcgggcagct acggtacatt gacaaccgaa gccatatacg acatcagcga
      601 cctccaaaac ccgcaactgg tgtggtcaca gccctacagc ctacaaagca ggatattttt
      661 caacccccgc gagccggagc atttctactg cctggctgct gatggcctgt ccgtcagcaa
      721 ataccaaatt tccggcggcc aggccagcct gctgttcagc catcccaatc ccgtggttct
      781 ccgtgatccg gtttttattc gcgggattct ctatcaggcc agcgtttccc cctcttcgcc
      841 agaccggtat gacctctatg ccactaacgg ttttgagcaa aacacacccc aagcgcctgt
      901 ggtgagggag ggattcatcc ctgggttatg ggcaatgctg gacgcgggaa actacttgta
      961 tatccgtgac tatcctggcc caacctgcat attccacaac gaattatcaa cctttgaagt
     1021 ggcaagcgac atcagggcat ttgccttcca cgattacgtc tgcgtgggcc ggcctgacgg
     1081 agtaagtttt tacgacacga cagttccgct aacgggattt cagacagagg acctgttcct
     1141 gcctcaatac tcatattccg tcaatatcgc ctgggacgat gagtatcttt atctcttcgg
     1201 gcaggacaac atcgccatct atgcctacac cctcaccacc ggtgccaacg gggaagaact
     1261 gcccaaacct ccggaaatcc agctttccgc cagtcccaat cccttttccg gcagcgtgga
     1321 gatcagtttt gagctcaaat cccctgccca agccgaactg gccgtttaca acctccgggg
     1381 acagaagcta cgcgatttat ccagcgcatc ttacggaccc ggcaaacaca gcttgacctg
     1441 ggacgggaag gaccattcag gaaccccggt tcccgccgga atctatctgc tcaggctgcg
     1501 cgccgagggg ctggaaacag ccttcaagaa aatcagcaag tactgaagta acaccattcc
     1561 ttacaagagg atatggtgtt agaaacctcc aaagtcaagg tgctggcgga gctgatcaag
     1621 gccgtctgaa gaggaaacag ttgcagtgtc ggggcgcaat ccgctatctc cgccctgcct
     1681 gccattggaa aaaacccagc aggcaaccgg ttcgtcctga agcctttatc atccgccatt
     1741 ggatagcctc ccgtataatc cccgcaccgg atgcggccat tatgcgagag gcgtgtggga
     1801 gctgaaggga agggcgttaa ggtggggcag aagcggttcc catagaccgc atccaggaat
     1861 cagtccggag ggcgacagaa ccaacttgat ttgatagaga accgcctttt caaccagcct
     1921 gccttttaac acatgaaaaa agcctccccc attttcggga gaggccttat atggatggta
     1981 ttggagttgt ttagtacatt cctccaccca tgccgggatt gggcattggg gcgggtggtt
     2041 cgggttcctt gatgtcggtg atgatgcact cggtggtgag catcagggcc gctatggagg
     2101 cggcgttttg caccgcggaa cgcacaacct tggcgggatc aatgatgccg gccttgatca
     2161 ggtcctcaaa cttgccgttg gcggcgttgt agcccatatg gatttccttg tatcctttca
     2221 gtttttccac gaccacagcg ccttcctcgc cagcgttcgc ggcaatctgg taggcggggc
     2281 gttccagggc tttcatcagg atttcaacgg ccatcttttc ctcgctgtcg aggtctttca
     2341 tcgtcttgag ggctttggca gcctggatca gggtgactcc gccgccgggc acgatgcctt
     2401 cctcaaccgc ggcgcgggtg gcgtgcaaag cgtcatccac gcgggctttc ttttccttca
     2461 tttcggtttc ggtggcggca ccgatgcgga tcacggcaac gccgctggaa agcttggcca
     2521 ggcgttcctg aagcttttcc ctgtcgtaat cggaggtggt ttcctcgatt tgggccttga
     2581 tctgcttcac gcgggcggaa acggcttcat cgctaccggc gccttcgcgg atggtggtgt
     2641 tctctttttc cacgatgatt ttcttggcgc ggccaaggtc ggtcatggtg gcgctgtcga
     2701 ggcggcgtcc catttcttcg gaaatcacgg tggctccggt gaggacggcg atgtcttcca
     2761 gcatggcttt gcggcggtcg ccaaaaccgg gggctttcac tgccaccacg ttgagcacgc
     2821 cgcggagctt gttaaccaca agggtggcca gggcttcgcc ttcgatgtct tcggcgatga
     2881 tcagcatggg gcgtccctgc tgggaaactt cctgcaggat gggcaacagg tctttgagga
     2941 cgcttatctt cttgtcgtga acaaggatga agggatcctc gagctcggcg atcatcttgt
     3001 cggcattggt tacaaaatag ggggaaaggt agccgcggtc aaactgcata ccttccactt
     3061 tttccagacc ggtgtcaatg gatttggctt cctcgatgtt gatgatgcct tccttgccta
     3121 cggattccat tgcttcggcg atcagtttgc cgatttcggg atcgttgttg gcagagatgg
     3181 aagcgatctg ggcgatctct tcattgctct tgatctcttt ggaatactcg tggattttat
     3241 cgaccacaac cttggtggcc ttttccagac cgcgtttcag atacatcgga tttactccgg
     3301 cggtcacgtg cttcaggcct tcctcgatga tggcctgggc cagcagggtg gctgtggtgg
     3361 tgccgtctcc ggcgttatca tgggtctttt ccgccacttc tttgcagagc tgggcgccca
     3421 tgttctcaaa ttcgccctca agctcgattt ccttggcgat ggtcacgccg tcgttggtga
     3481 tggtggggga accgtatttc ttgtccagga caacgtttct gccgcgcgga ccaagggtca
     3541 ccttcacggc gtcggcgagc tggtcaactc ccttcttcag ggaagtgcgg gcttcgtgtg
     3601 aatatagcat ttgttttgcc attgatttat acctccatat ggtttaatct gtggatatta
     3661 atttagcagt ctcagtaact gagtgctaaa actaagataa gctgaattct gtcaagcaca
     3721 aattttattt tccgtttgac agatagccgc caccgggaaa gctggccgta aaagacgata
     3781 cggaacacag atggacaaac acatagaatc agctctcgcc cagcttttac cggaacttgc
     3841 ccggcatggg atcagcgttt ccagccaaag ggagcttcaa tacggactgc agttgcggct
     3901 ggagcgcgac gccgaaaccg ccaccctgaa tctctactgg tcgctgaaaa agggaataag
     3961 ccaagtcctg gcgggcaagc ccaaccgcct gaaagctgac ctggccaagc ttctgaatgc
     4021 tgccgaagcc gggccggtgg gcgattttca cacctgggaa cgctggatcg gatctgatga
     4081 atgcgggaaa ggcgattatt tcggggctct ggtggtggcg gctttcgccc tggatgccga
     4141 aaaagttgat gaggtccgct ccctgggcgt tttggattcc aagaaactca ccgatatcca
     4201 ggtcagggac atcgctcaca acctctactc gcgcttcccg gaacggatcg cctgcatcgt
     4261 catcaagccg ctgcgctaca acgagataat ggccgacagg cagagccgcg gggagaacct
     4321 gaacgacctc ctggcttggc agcacggcgc cgcgatcatg gaattactgg gacggatacc
     4381 tctggtgcag ggtgtgctgg tggaccagtt cagcctgcat aaaaaggtaa gcaagtacct
     4441 gaaaaacaag gaaatcggca ttcccgtgga agagcgtccc aaagccgagg ccgatcccgc
     4501 cgtggctgcc gcttccatta tagcccgcta ccagtttttg cagagccgcc aggccatgga
     4561 ccggcattac aagatgagtt ttcctctcgg aagcggcaaa aacgtgcttc cgcccgccaa
     4621 ggcttttgcc gacaaatacg gttgggaccg ccttgccgag gttgccaagc tgcacttcgt
     4681 caccagccgc ggcgttcggg ttaaggatat tttcgaccct cccgctgata aatagcttga
     4741 cagaatggaa cacagctaaa acttgaatcc ataaatcaaa tccaaacggc aaaactccca
     4801 agttcctcaa acggaatcaa ggcatattgt aacatttttt gcacctcaga gaaacatacc
     4861 atcccccacc gggattttgg agcataccat tatgatcaaa aagatgggac gtctgaatct
     4921 ggacgactat agaattatgg acgctcatgt ggacaagatt ttcaagaatc tggacaaggg
     4981 caacaacggc atagcggaac ggcagatatt tgatatggcc aacacccaaa actacttcgt
     5041 gcgtgaagaa ttgggcaaac gcctggccgt ttatgaaggc aagggcaagc tggaacgcat
     5101 ctgcggcgat atgctggagc ataatctcta cggcatccgc gccaccgccc tcttctattt
     5161 ttactaccgc caccagtccg agcccgccat catcgcccaa accctggaca aaacctgcga
     5221 aaccgtgccc tgggaaagcg agaccatctg tttcgagatg tggaaaaagg ctcccgaggt
     5281 gatgcaggaa tacatgcccc gctggtccac atccgaaaat cccaaaaagc gcgagatgtc
     5341 gctccacggc atggaaaaca tcgcttcccg cagcccccag tatgtgctta ccttcatcag
     5401 ccgtctgcta gatgacgagg atgaggaagt gcagaaaaag ataagccaca tcctggtgca
     5461 ggtgggccgc caacgcccgc tgcaaaccta tgccagcatt cgccgctggc tgctggaagg
     5521 cgatgaaacg cgtgcccgca ccatctgggc caccctcaaa aaactcacca gtatcttttc
     5581 ccagaagaac atgcgcgaca aggcccagga gttcgtgagc atttcccaac gcgttgtgaa
     5641 aggctggaaa tccgattccg acccggctgt ggtgcaatta gggcacaaac tgggcaaact
     5701 gatcaaggat atatgatcca cgtaatcctg gtggagccta tctatgaggg aaatgtgggc
     5761 gccgtggcca ggattatgca caacttcggt ttcacagggc tgcggatcgt gggcgctgtg
     5821 cccagcaaga acgattttta cctggccatg cattcagaac acttgctgga aaatgctttg
     5881 gtctttccca ccctggcaga ggctgtgact gatctggatc gcgtcatcgc cttttcccgc
     5941 cgccttggca aactcaagcc ggcggacctc aacccgcccc agatggcgga ttattgcacg
     6001 aaaatccccc aactggagat tggcctggtc ttcggccgag aaacttttgg cctcacagac
     6061 gccgaagcag ctctctgccc cctccgctgc cacattccgg ccaatccggc ctttccctcc
     6121 atcaacctcg cccaggccgt gaccattgcc ctttgggaac taagccgtgg aggagaaaaa
     6181 agcgcccgga tcaaagccgt gagcggaatt gaacttgaca aaatgaaagc ctacatgctt
     6241 gatgtcatgg aggccacagg attctttcgc agtcacgaga ccacgaactg ggaaatgttc
     6301 atcgccaaga tgctcgccca actgaatccg accaaaacaa tgctttggcg tttccgccag
     6361 atgttcaacc gctggcatgt gctcgtcaca ggcaggggca gaggttatga acgctccctc
     6421 ccggccgggc aggataccga agacaaacca gaataagcat tcgctatatg ccgccggtgc
     6481 tctatctgcc tggcggaccc agtttaatag aggtaagcta tgacactttt aacggcaaga
     6541 accgagctcg atccccgtca gcaggagatc ttcaaattct gcgaagccta tttcttcgcc
     6601 catgaaaatc ccgccctggt gaacaagaac gcccgctact tcacagaggg ttacgatgct
     6661 tttggcatca aagaggacga gcttcgcgag ctccgagacc aactgctgga acgctttgaa
     6721 cccagcgttc ccgagctggc ggaactcgct taccaccttt tcgccaccgg taaatacgaa
     6781 ttcggctcgc tggccttgat gctgcttaaa aaacaccgac cccgcttcga ccgcagcgtc
     6841 tttgaccagc tcaaacgtac cttggacgat gtggtggaga actgggcaca tgctgatgtg
     6901 atcggcacaa aactcactcc cgtgctgctg gagctgaatt tggttgacct cgcggatttc
     6961 gagccctggc gcttttctcc cagtaaatgg acccgccgtg ttgctgctgt caccttgctt
     7021 tacctgcgca gccaccgacc tgtggagcaa ttgctggaat tcatccagcc cctgatgcag
     7081 gacaataccc ggccagttca acagggcgtt ggctggttcc tgcgcgagct ctggaaagtg
     7141 cattcccgcg aagtggagga tttccttttt gctaacaagg ataccgcgcc ccgcctcgtg
     7201 atccagtatg ctacggaaaa gatgaacaag gataaaaaga agcgcttccg ccgcgccatc
     7261 agcaaggctg tgaaaccgaa caagcatcaa tcccgcaagg acaaaccacc ccaggaggat
     7321 tcaggtgaat aagctttttc cgatcatttt cttgctctgc ttaccgctgc tgctcccggc
     7381 acagcaactc tctgtttcac ttgacctttc cgcactcacg cccggcaccg atgagctccc
     7441 tgccggatgg ggagtcctca ccgcacccgg ctttccacgc ctgcctgtca agacagtgaa
     7501 cattgtcctg accccggagg ctgccaacct cactttcagt tatcagttca ccggccttaa
     7561 aagcgttccc tctccgcctc cctttctgaa tccgcctttt acagacggag aatgtgttat
     7621 cgccggccca gcctccttat caggtgctgc cagggttgtc tacaagggca tcggccattg
     7681 gggcgaagtg gcttacgcca gcttttcagt tcttccggcc acgcatactg gagctgaatg
     7741 gcaggcctgg cagcaacttc ggctcgacct cagttgggag cagcccgcag aaactgcctc
     7801 aaaaacactg cctcctgtc
//
LOCUS       DAOOWR010000031         7807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9303_length_7807_cov_3.580495, whole genome shotgun sequence.
ACCESSION   DAOOWR010000031 DAOOWR010000000
VERSION     DAOOWR010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9303_length_7807_cov_3.580495"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLB85_01555"
     CDS             <1..431
                     /locus_tag="PLB85_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU72041.1"
                     /translation="WIKLATQKLALEICDVMDNFERALNQVEEAAREDAFVKGVTQIE
                     QQLRGVLGKEGVCRIDALGKEFDPALHEALAHIPSEYEANLVAAVIQNGYTMHDKVLR
                     PVRVAVSSGKYEANQAETDPPVEDKPSSGNGSDSINIEIK"
     gene            454..2403
                     /gene="dnaK"
                     /locus_tag="PLB85_01560"
     CDS             454..2403
                     /gene="dnaK"
                     /locus_tag="PLB85_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU72042.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGGRTTPSVVGFTK
                     DGQRLVGQLAKRQAVTNPINTVFSIKRFMGRSFSEVGSEMKQVPFKVKSGLKGQAAVH
                     IDTDNKDYTPQEISAMVLAKMKETAEVYLGQSVTEAVITVPAYFNDDQRQATKDAGKI
                     AGLDVKRIINEPTAAALAYGMENKKEQKVAVYDFGGGTFDISILDISQGVVEVLSTNG
                     DTHLGGDDFDKCVIDWMLDQFKKLEGIDLSKDPIAMQRLREAAEKAKIELSGTQTTNI
                     NLPFITADATGPKHLSIDLSLAEFNRLTENLVQRTLGPCKQALADAKVSASDINEVLL
                     VGGSTRIPAVQEAVEKFFGKKPNRSLNPDEVVAIGAAIQGAILSGDEKVSDVLLLDVT
                     PLTLGIETLGSVMTSLIPRNTTIPTKKSQVFSTAADNQTAVTIHVLQGERPMAQDNKS
                     LGRFDLVGIPAAPRGIPQIEVTFDIDANGILHVSAKDMGTGKEQSIHIDRAGDMSKDD
                     IDRMIKEAEMHAEEDKKRQEFISARNELDGLIFGTEKSLREYGDKLSESEKSELEAEL
                     KNAKESMEKATEASEIESIKQNLAQKAQRLGEIIYAQAQQQQGTEGPNTSGFDQQGFQ
                     GEAPAEEAQQSEGPIDADFEVVDDK"
     gene            2473..3630
                     /gene="dnaJ"
                     /locus_tag="PLB85_01565"
     CDS             2473..3630
                     /gene="dnaJ"
                     /locus_tag="PLB85_01565"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU72043.1"
                     /translation="MAKRDYYEVLGVPKTADEAEIKKAYRKLAMQFHPDKNPDNKEAE
                     EKFKEASEAYEVLSDKDKRQIYDQYGHAGIDQQFGSGGFNWENFSRFEDISDLFGGGS
                     FGSIFDTIFGGGFGRSGGQSSRSNKGSDLRIELSLSLKEIALGTEKTVKINAKTACDA
                     CNGSGSADGNTETCTQCRGTGQVRTVRQSLFGQMQTVSECPSCRGEGKIIRNKCSKCY
                     GEGRMAKTKEIKVKIPGGMEEGQILRVRGQGHIGLRNGPHGDLLVQVNERRDVLFERY
                     GNNVVLEFPVHFTQAVLGDEIIVPTITGKARMKVPAGTQSGREFRLKGEGIQGLYSDS
                     RGDLIVRINVHTPSRLSREETELYKRLADLNKKRELKPGKTFKEKLKDFFT"
     gene            3644..4369
                     /locus_tag="PLB85_01570"
     CDS             3644..4369
                     /locus_tag="PLB85_01570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOU72044.1"
                     /translation="MPSVYYPGLERLQTDSLVRLEGEEFHHLARVAKRRLKDILLLNS
                     GKGTLATAKVEKLEAHSVLLRLTEIKFPEERRHPFAIAFALLRNRNDELLVEKCTELG
                     VSALFPLITEHSIHQAMDNTITRFGRISLAAAKQCDNPWLPEIRHPQKLETALETIRE
                     AGYTPILCSERKPDRWLHHLTEEEVGRPCFLIGPEGGWSEAEFKLMEGLTEITLGDLI
                     TRAETAGIVVAAQWQAFANNLDQ"
     gene            complement(4649..5716)
                     /gene="lpxK"
                     /locus_tag="PLB85_01575"
     CDS             complement(4649..5716)
                     /gene="lpxK"
                     /locus_tag="PLB85_01575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOU72045.1"
                     /translation="MTDFADRHLKRPSPLSDLLSPLGRINSLLQTQRRKRLSNNAWRP
                     HCKVISIGNIVCGGSGKTPFTIYLAALMQASGYRVGVSHRGYKGGLEKSAPTLISGRD
                     GLLHEVEAAGDEALLIASRLPGIPVVVGKHRVAAVSLLLAKYPDLDAVLLDDAFQHLS
                     IARDLDVVCFEADTGCGNGRVLPAGYLREPLTALNFASLIVVNHKHPYRETEAGSDFW
                     AEFRKPLLHYRLQPRDVVSADGKEMEISSLRRKKLLLISGIANPDSFEATLKAAGLSW
                     LHHFRFADHYAFTNKAEIRRMACLARKYGAETLLCTEKDLAKLARHGEIRDMLLALRM
                     DLVCEDEEALMGLVKLKVPLS"
     gene            complement(5717..6097)
                     /locus_tag="PLB85_01580"
     CDS             complement(5717..6097)
                     /locus_tag="PLB85_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU72046.1"
                     /translation="MPKRICIVDDEESIIAGLKQELETLRPAWKVVGFNSGLNAIREI
                     ISGGIDLVITDIAMPDMDGYELFWRIKDHDPSLPVIMMTGFGYDPNHVLVRAKTDGLQ
                     DILFKPFDIDKLIALIESKLEPEG"
     gene            complement(6101..6976)
                     /locus_tag="PLB85_01585"
     CDS             complement(6101..6976)
                     /locus_tag="PLB85_01585"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU72047.1"
                     /translation="MYEQIDELDIIFSSHQGKVRDIHDLGDKLLIVTSDRISAFDVVF
                     PDPLPGKGVILNQIAAHIFRTTAHIVPNHFITDKIEDYPPELMPFKSYLEGRSMLVKK
                     LRVIPVECIVRGYISGSAWSEYQQSRSIGGMAIEEDLRESQKFAQPLFTPSTKASTGH
                     DVNISFEQMKQRMDPAIADYIKEKSLELYKWAHAKLLDKGIVLADTKFEFGALGSDII
                     LADEALTPDSSRFWDLSLYEVGHSPASFDKQIVRDYLISTGWDKQPPAPCLPEEIVHK
                     ALEKYRQIRDLIVGD"
     gene            complement(6980..>7807)
                     /locus_tag="PLB85_01590"
     CDS             complement(6980..>7807)
                     /locus_tag="PLB85_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72048.1"
                     /translation="VAQRLKVIRQKAENDSRYGENSSAADTLRIESYPRAIDALIRFK
                     VFLSKQQPRFQHAMRTSSDYVYLALDARRFPFVCVKEDTNDDWTYLGPWRGRFFLTDV
                     IDSFARILKLPNCETDSHPCEKLESGACQGWCQAFAEDLSEDQKPDLEKLETLLRETY
                     LHPQNGILEMISKERDRYFDDLEFAKADLLDDEIEKLTQYRDWLNFLYVTKSLNYNEE
                     KFGVMNGLMSWCLYEGMSYEFANAPVSYRDNELLAINLIDTDEARLLYEYHVKHRKG"
BASE COUNT         1907 a         2097 c         2128 g         1675 t
ORIGIN      
        1 attggatcaa gctggccacg cagaaactgg ctttggagat ttgcgacgtg atggacaact
       61 tcgagcgcgc cctgaaccag gttgaagaag ctgcgcgcga agacgccttc gtgaagggcg
      121 taacgcagat cgaacagcaa ttgcgcggtg tccttggcaa ggaaggcgtg tgcaggatcg
      181 atgccctcgg aaaggagttc gatcccgcgc tgcatgaggc tttggcacac attcccagcg
      241 aatatgaggc caaccttgtg gccgctgtca tccaaaatgg ctacaccatg cacgacaagg
      301 tgcttcgccc cgtacgtgtg gccgtttctt caggcaaata tgaagccaat caggcggaaa
      361 ccgatccgcc cgtggaggat aagccttcca gcggcaatgg ttccgatagc ataaacattg
      421 aaattaaata aaagtaccat ggaggaataa gaaatgggaa aaataatcgg aatagatctg
      481 ggtaccacca actcctgcgt agcagttgta gaaggtggca aacccgtcgt aatcaccaac
      541 gctgaaggtg gtcgcaccac cccttcggtg gtaggtttca ctaaggacgg acagcgtctt
      601 gttggccaac tggccaagcg tcaggctgtc accaacccca tcaacacggt gttttcgatc
      661 aaacgcttca tgggccgctc tttcagcgaa gtcggctccg aaatgaagca ggtgccgttc
      721 aaagtgaagt ccggcctcaa aggccaggcg gctgtgcata tcgacaccga caacaaggat
      781 tacaccccgc aggaaatctc cgcgatggtg ctcgcgaaga tgaaggagac cgcggaagtc
      841 tatctgggac agagcgtcac ggaagccgtg atcaccgttc ccgcctattt caatgacgac
      901 cagcgccagg ccaccaaaga cgccggcaag atagccgggc tggacgtgaa gcgcatcatc
      961 aacgagccca cggccgcggc tttggcttat ggcatggaaa acaaaaaaga acagaaagta
     1021 gccgtttacg actttggcgg cggcaccttc gacatctcca tcctcgacat atcacagggc
     1081 gtggttgaag tgctctccac caacggcgac acccatcttg gcggagacga ctttgataaa
     1141 tgcgtcatcg actggatgct ggatcagttc aagaaactgg aaggcatcga cctttccaag
     1201 gatccgattg cgatgcagcg cctgcgcgaa gccgctgaaa aagccaaaat cgagctttcg
     1261 ggcacgcaga caaccaacat caacctgccc ttcatcactg ccgatgccac cggccccaag
     1321 catctgagca tcgacctcag cctggctgaa ttcaaccgtc tcacggaaaa ccttgtccag
     1381 cgtacgctcg gaccctgcaa acaagccctg gcggatgcca aggtgagcgc ctctgatatc
     1441 aatgaagtgc tgctcgtggg cggaagcaca cgcatccccg cagtgcagga agcggtggag
     1501 aaattctttg gcaaaaagcc gaaccgcagc ctcaatcctg acgaagtggt ggccatcggt
     1561 gccgccatcc agggcgccat cctttccggc gacgaaaagg ttagcgatgt gctgctgctc
     1621 gacgttacgc ccctcaccct gggaatcgag accctcggca gcgtgatgac gtcactcatt
     1681 ccgcgcaaca ccaccatccc cacgaaaaaa agccaggtgt tttccaccgc cgcggacaac
     1741 cagacagcgg tcaccataca tgtgctgcag ggtgagcgtc ccatggcgca agataacaag
     1801 tcccttggcc gtttcgacct tgtcggcatt cctgccgccc cccgcgggat tccgcagatc
     1861 gaagtcacct ttgatatcga cgccaacggc atcctgcacg tttccgccaa ggacatgggc
     1921 accggcaaag agcaatccat ccacatcgac cgcgccggtg acatgagcaa ggatgacatc
     1981 gaccgcatga ttaaagaagc ggaaatgcac gctgaggaag acaagaagcg gcaggaattc
     2041 atctccgcac gcaatgagct cgacggcctc attttcggca ctgaaaaaag cctgcgcgaa
     2101 tatggcgaca agctgagcga aagtgagaag agcgagctcg aagcagagct gaagaacgcc
     2161 aaagagagta tggaaaaagc aactgaggct tccgaaatcg aatcaatcaa gcagaacctg
     2221 gcacagaaag cgcagcgcct cggcgagatc atctacgctc aggcgcagca gcagcaaggt
     2281 acggaaggac caaacacctc cggctttgac caacagggtt tccagggaga agcgcctgcc
     2341 gaagaagcgc aacaatctga aggcccaatc gacgccgatt tcgaagttgt ggatgataaa
     2401 taaatatgaa aggtcccggc ggggaatatc tttcgccggg gcctcacctt attcgcgagc
     2461 agaggagtaa aagtggcgaa acgagattat tacgaagttt tgggcgtgcc caagacggca
     2521 gatgaagccg agatcaaaaa agcgtatcgc aaactggcga tgcagttcca tcctgacaag
     2581 aatccggaca acaaggaagc ggaggagaag ttcaaggaag cctccgaagc ctacgaggtc
     2641 ctttccgaca aggacaagcg ccagatttac gatcagtatg ggcacgcggg catcgatcaa
     2701 cagtttggca gcggcggctt caactgggaa aatttcagcc gctttgaaga catcagcgac
     2761 ctctttggcg gtgggagctt cggctccatc ttcgacacca tctttggcgg aggttttgga
     2821 agaagtggag ggcagtcgtc gcgctccaac aagggcagcg atttgcgcat cgagctctca
     2881 ctcagcctga aagaaatcgc cctcggcacc gaaaagaccg taaagatcaa cgccaaaaca
     2941 gcttgcgacg cctgcaacgg cagcggcagc gcggatggaa acaccgaaac ctgcacccaa
     3001 tgccgcggca ctggtcaggt gcgaacagtg cgccaatctc tgttcggaca gatgcagacg
     3061 gtctccgaat gtccttcctg ccgcggtgaa ggcaagatca tccgaaataa atgctccaaa
     3121 tgctacggcg aaggtcgaat ggccaagacg aaagaaatca aggtcaagat tcccggcggc
     3181 atggaggaag gacaaatcct gcgtgtgcgc ggtcagggcc atatcggcct gcgcaacggc
     3241 ccccacggcg atttgctggt gcaggtgaac gaaaggcggg acgttctttt cgaacgctac
     3301 ggcaacaatg tggtcctcga gtttcctgtg catttcacgc aggctgtgct gggagacgaa
     3361 atcatcgtgc ccaccatcac cggcaaagcc aggatgaaag ttcccgccgg aacccagagc
     3421 ggaagggaat tccgtctcaa gggggaaggc atccagggcc tttacagcga ttcccgcggt
     3481 gacctcatag tgcggataaa cgtccatact cccagccggc tatcacgcga ggaaacagag
     3541 ctttacaaac gtctcgctga tctcaacaag aagcgcgaac ttaagcccgg caagaccttc
     3601 aaggaaaagc tgaaggattt cttcacataa tcagagtagc gcgatgccgt ctgtttatta
     3661 tcccggtctg gaaaggctgc aaacggatag tcttgtaagg ctcgaaggcg aggaattcca
     3721 tcatttggca cgggtggcca aacgccgcct aaaagatatt ttgctgctta attcgggcaa
     3781 aggcactctc gccaccgcaa aggtggaaaa actggaggcg cactcggtcc tgttgaggct
     3841 cacggagata aaattcccag aagagcggag gcatcccttc gcgatagctt tcgccctcct
     3901 caggaaccga aacgatgagc tgctggtgga aaaatgcacc gaacttggcg tatcggcttt
     3961 gtttcctctc atcacggagc attcgataca ccaggccatg gacaatacca taacccgttt
     4021 tggcaggatc agccttgccg ccgccaagca atgcgacaat ccctggctgc ctgagatacg
     4081 tcatccgcaa aagctcgaaa ccgctttgga aaccattcgc gaagccggtt atactcccat
     4141 cctctgctcg gaacgcaaac cggatagatg gttgcatcat ttgacggagg aagaggtggg
     4201 acggccatgt ttcctgatcg gaccggaagg tggatggagc gaggcggaat tcaagctgat
     4261 ggagggtttg acagagatca ctttggggga tcttatcacg cgggcggaaa cggctggcat
     4321 cgtggtcgcg gctcagtggc aagcctttgc caataatctg gatcaatagc cccttgtggg
     4381 cgtttgaaag agagcctttg taagagaaac ctatttcata gagagtaaaa gctactttcc
     4441 ccacagcaat atcctgcctg ttgcgtcctt agcgcccgaa ttcactcccc tctcacgcca
     4501 cgcgcaaaat actcgcatca aatgcgggca tcatgtggga ggcgcttcag ggacacgggg
     4561 caaggccttc atggccagac ggcattgctt cttcccggtt acttcaaaca tcaattaaaa
     4621 cattcatacc cagggcgtta tctgtatgtt atgaaagagg aactttaagc tttaccaacc
     4681 ccatcaatgc ctcttcgtcc tcgcaaacga gatccatcct caatgccagc agcatatccc
     4741 ttatttcccc gtgccgcgcc agtttcgcca gatccttttc tgtgcaaagc agcgtttcgg
     4801 ctccgtattt tctagccagg caagccattc gcctgatctc tgctttgttt gtgaaagcgt
     4861 agtggtcggc aaaccggaag tgatgcagcc agcttagccc tgctgctttc agagttgctt
     4921 caaacgagtc gggatttgct attccgctta tcagcagcag ctttttcctc ctcaaactgc
     4981 tgatttccat ctctttgcca tcagccgaga ccacatcccg cggttgcagc ctgtagtgga
     5041 gcaagggttt gcgaaattca gcccaaaagt cactaccagc ttctgtttcg cggtaagggt
     5101 gtttgtggtt gacgacaatc agggaagcga agttgagggc agtgagaggt tcgcgcaaat
     5161 atcctgctgg cagaaccctt ccgttgccgc agccggtgtc cgcttcaaag cagactacat
     5221 ccaggtcgcg ggcgatgctg aggtgctgaa acgcgtcgtc cagaagcacc gcgtcaagat
     5281 cgggatactt agccagcaac agggaaactg ctgccacacg gtgttttccc accaccacgg
     5341 ggatgccggg cagacggctg gcgatgagca gagcttcgtc gcccgcagct tcaacttcat
     5401 gcagtaagcc atccctgcct gaaatcagcg tgggagcgga tttttccagt ccgcccttat
     5461 agcctctgtg cgaaactccg accctgtatc cggaagcttg cataagggcg gcaagataga
     5521 tggtgaaagg tgtttttccg ctgcctccgc acacgatgtt accgatgctt atcaccttgc
     5581 aatgtggacg ccaggcattg ttggaaagcc gcttacggcg ctgtgtctgt aaaagggagt
     5641 tgatccggcc aaggggactg agaaggtcgc tgagcggtga ggggcgttta aggtgccggt
     5701 ctgcgaaatc cgtcaatcaa ccttccggtt caagtttgct ttcgatcaac gctatcagtt
     5761 tgtcgatgtc gaacggtttg aacagaatat cctgcaggcc gtcggttttg gcccgcacca
     5821 gcacgtggtt ggggtcgtag ccaaagcccg tcatcatgat cacgggcagg ctgggatcgt
     5881 ggtcttttat acgccagaaa agctcgtagc cgtccatgtc cggcatggct atatctgtga
     5941 taacgaggtc aatgccgccg ctgatgatct ctctgatggc gttcaaaccg ctgttgaacc
     6001 ccaccacttt ccaggcgggt cggagcgtct ccaattcctg cttcagaccg gcgatgatgc
     6061 tttcttcgtc atccactatg catattcttt tgggcatgga tcaatctcca acgattaggt
     6121 ctcggatttg ccggtatttc tcaagtgctt tatgcacaat ttcttccggc aggcacggcg
     6181 cgggcggttg tttgtcccaa cctgtgctga tgaggtaatc gcgcacgatc tgcttatcaa
     6241 agctggcagg actatgccca acctcataca gcgaaaggtc ccaaaagcgc gaggaatcag
     6301 gggtaagggc ttcatcggcg aggattatat cgctgccgag ggctccgaat tcgaatttgg
     6361 tatcggccaa tacgatgcct ttatccagca gcttcgcgtg tgcccatttg tatagttcga
     6421 gggatttttc cttgatgtaa tcggcaatgg cgggatccat gcgctgtttc atctgctcga
     6481 agctgatgtt cacgtcatgg cctgttgagg ccttggttga tggagtgaaa agcggttggg
     6541 cgaatttctg gctttcgcgc aaatcttctt ctatggccat gccgccaatg ctgcgcgact
     6601 gctgatattc gctccaggca gagccgctga tgtagccgcg cacgatgcat tccacgggga
     6661 tgacgcgcag ctttttcacc aacatgctgc gtccctcaag ataactctta aaaggcatca
     6721 gttcaggcgg atagtcctcg attttgtcgg tgatgaaatg gttgggaacg atgtgcgcgg
     6781 tagttctgaa gatgtgcgcc gcgatctgat tgaggatcac tcccttgccg ggaagcggat
     6841 cggggaaaac cacatcgaag gcggaaatgc gatcactggt gacgatcagc agtttgtcgc
     6901 caaggtcgtg aatatcccga actttgcctt gatggctgct aaatataata tccaactcat
     6961 ctatttgttc gtacatagac tatcctttac gatgtttaac atgatattca tagagcaatc
     7021 ttgcttcatc ggtgtcgatt aggttgatcg ccagcagctc gttgtcccga taggagacag
     7081 gcgcgttggc gaattcgtag ctcatccctt catacagaca ccagctcata aggccgttca
     7141 tcacaccgaa tttctcttca ttataattca aagacttggt cacgtaaagg aaattgagcc
     7201 agtcgcggta ttgggtcagt ttttcgatct cgtcatccag caggtccgcc ttggcaaatt
     7261 cgagatcatc gaaatagcgg tcgcgttctt ttgagatcat ctccagaatg ccgttttggg
     7321 gatgcagata tgtttcgcgc agaagggtct cgagcttttc cagatcaggt ttctggtctt
     7381 cgctaagatc ctccgcgaag gcctggcacc agccctggca ggcgccgctt tccagcttct
     7441 cgcagggatg gctgtcagtt tcgcagttgg gtagcttcag gatgcgcgcg aagctgtcta
     7501 tcacatcggt aaggaaaaag cgtccgcgcc acggccccag atatgtccag tcgtcgttcg
     7561 tgtcctcttt tacgcagaca aaggggaaac ggcgggcatc aagggcaaga tatacatagt
     7621 cactgctggt ccgcatggca tgctggaaac ggggctgttg cttgcttaga aagactttga
     7681 agcggattag ggcgtcgatc gcccgcggat agctttctat ccgcaaagta tccgcggcag
     7741 aggaattttc cccatagcgg gagtcgtttt ccgctttctg gcggatgacc ttcaaacgct
     7801 gtgccac
//
LOCUS       DAOOWR010000032         7771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9367_length_7771_cov_3.579057, whole genome shotgun sequence.
ACCESSION   DAOOWR010000032 DAOOWR010000000
VERSION     DAOOWR010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9367_length_7771_cov_3.579057"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PLB85_01595"
     CDS             <1..610
                     /locus_tag="PLB85_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU72049.1"
                     /translation="QNYISNPKVLRRLKELLAGEEVGLHGSPESAFDEEVLRAEMDRL
                     VKAGFEVRGYRSHYLHFDYQKSFRILENAGILYDSTLGYWENIGFRAGISFPFYPFNI
                     EENRPFRVLEIPLIVMDTTLHSKKAMNMNPLAARLTLRRLIDMADKYQSHLSLLWHNV
                     TFDPIDFPLWAGVYWSTLRYAISKGGWITSLHDIWTEWTYFD"
     gene            624..1349
                     /locus_tag="PLB85_01600"
     CDS             624..1349
                     /locus_tag="PLB85_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU72050.1"
                     /translation="MFNKLTSILWKVFFVIMMLLLFIFLLFHLVTKLFLSPSRYRRLT
                     WQVARVWGWFTVVSTGTRVKVSGRENIPLEDPICFMGNHQSYFDIPTLLGFAGCPMGF
                     IAKQELARVPVLKQWMVQLPSFFLDRDNARQAIKVFQAAAKVMKEGHPMVIFPEGLRA
                     ERGKLADLHLGSLKLAQMAGATIVPFALDGTWRMLEIDGDIHAARVNFTILPPVRPGD
                     PIYDDKIALAAHLKSSIEACLTD"
     gene            complement(1491..3404)
                     /locus_tag="PLB85_01605"
     CDS             complement(1491..3404)
                     /locus_tag="PLB85_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU72051.1"
                     /translation="MNTNKKTKYLGQQLISFMFIFMLILFLVTFLITRQLAYKVMRSN
                     AEESISNLTETNAQMIDKNLSNIMTIAQELRTVINDDLYTPKQLKRHIHHLLIDNPRL
                     ISVCLAYDKPSTKRTQTFYLENQQIRSIPAEDDDYLYKDWFQIPWLSLRPWWSDPWFD
                     NLGSKERVCSYSLPLTIKGKHQGIIRLDTPMESLHRIVKPIRVKKTGFAFMIASNGMI
                     VAHPRDSLAMNYSAFDFANRYGDQELRQICKNAVSGQSGFERFRFEKPGPDVWIAYRP
                     LPSNHWSLLVEVPNSEVFADLHSLSIITTIAFVLAFLIFSGTIWYRTSALNQPLSELV
                     DAIKLAGEGELQSGPEIDSNTYEIQVIAENFEKMKSSLTSHINNLQQVTEEKNRIMAE
                     VAFASIIQRNLIPKNDDASKLPANLKAFGILEPAGAIGGDLYDYFLKDDKHFLFAIAD
                     VAGKGVAAAMTMTMMTTLLRTVSSDKDKPEDILHILNTFLVENNPKSDFVTMVLGIID
                     LESGSCVFSNAGHTPIYQLKAAGGFSKFATTHSTALGFFDNIKITSDVLQLAPGDKIL
                     VFTDGVSEAVDRHDKLFGTDGLEKVLNKEAGSSPEETVQAVLAAVGEFADTQKSHDDT
                     TILVVEFLKPLNC"
     gene            complement(3391..4308)
                     /locus_tag="PLB85_01610"
     CDS             complement(3391..4308)
                     /locus_tag="PLB85_01610"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU72052.1"
                     /translation="MEEVQPGELFKLRYRLKWLHQAQFAGAYMAKEKGFYQAWGLDVE
                     FLPGGGDHPPYQSLMEGSADIASFNLVTALKYHNPKNPAVLMAQISQKNSTVLVGKKS
                     SGIKTIQDLDGKKIGVWREEGGDHTLFFLESLGLNIRAIPMDWSVNLLLENAVDMINA
                     MYYNEYHRLLMTGLDEEDLVVFDLADYGFDLVDDGIYTTRDFFEKHPRQCRDFTAATL
                     DGWNYAFQNPEETLAVVLRYLRGNHQPANLEHQAWMLEHIKSRILENPARVGYLDPKD
                     FDLANRILIERGIIDKPVDYHTFYPHEHK"
     gene            complement(4613..6226)
                     /locus_tag="PLB85_01615"
     CDS             complement(4613..6226)
                     /locus_tag="PLB85_01615"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU72053.1"
                     /translation="MAKYIFVIGGVLSSLGKGIATASIGLLLKSMGYRVATQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVDEALTRHSSCSAGQIYERVLQNE
                     RKGIYKGKTVQVIPHVTDEIKRRIQKLDADYDIIITEIGGTVGDIEILPFLEAVRQLR
                     LDLGLQNTLYVMLTYIPFIHAAGELKTKPSQHSAYKLREIGIQPEILLCRSEKPFESE
                     IYDKIALFTNVPRANVINAIDVKSVYECPITFKNANLHKIICRHFGLECKPLRLTAWN
                     KFLQNSTSWEQKVTIAVCGKYVKHQDAYKSVEEALFHAAAHHRMGLQVKWVNSEQKFK
                     PSSLAKILKDVDGILIPGGFGVKGIEGKIAIAEYARTRNIPFLGICLGMQVAVIEFAR
                     HVCHLKDANSTEFFADTPHPVIHKMEDSIYQKLTGGTMRRGAYPCNLEPGSLARKAYG
                     RARISERHRHRYEFNNAYRDKLTSKGMCLSGFSPDGLLVEVVELPKKGFYLGVQFHPE
                     FKSRPNKAHPLFRDFFGAAHAYSSGKSKQ"
     gene            complement(6293..7537)
                     /locus_tag="PLB85_01620"
     CDS             complement(6293..7537)
                     /locus_tag="PLB85_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU72054.1"
                     /translation="MADKLKLDKSMALYAEAKTLVPGAVAGIRRPYNFVEGEYPIFFE
                     EGKGGRIRDVDGNEYIDYLCAYGPIIIGYREEEIDNAVIEQIKNKGFCFSLTQSIQNK
                     LVQKLQEVVPCCEMAAIVKTGSDATTISIRVARAYTGKVKVARCGYHGWHDWCVEVKG
                     GIPEKFYEDVIEFHYNDLDQLEDILKANANDMAAIIVTPVGHPLAAEVQMPKPGYLEG
                     VRKLADKYGCVLIFDEIRSGFRCSLGGAQELFGVTPDLSTFGKAMANGYPIAALVGKR
                     EVMDVLTKDVFLSSTFFPNSDAQIAALKTIEILQRDKILDVVAAKGKKFGEDVEKVVA
                     ESGIPARFSGSPWMPFITFPRDEEGLYKKLRVEFYTQLIRRGVFLQPYHHGYICYRHT
                     DADLAFTVQAIKESLAELKKLA"
     gene            complement(7539..>7771)
                     /locus_tag="PLB85_01625"
     CDS             complement(7539..>7771)
                     /locus_tag="PLB85_01625"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOU72055.1"
                     /translation="LAVFKGLEMEGVGKLDMDKVNVNGGAIALGHPIGSSGARIIVTL
                     LHEMGKRNLNKGLAALCIGGGMGIATLWERSN"
BASE COUNT         1738 a         1938 c         2101 g         1994 t
ORIGIN      
        1 ccaaaactac atatccaacc ccaaggtgct gaggcggctc aaagaattgc tggccggcga
       61 ggaagtgggg ctgcatggca gtccggaatc agctttcgac gaggaagtcc tccgagcaga
      121 gatggaccgt ctggtcaaag cgggatttga ggtgcgcgga taccgatccc attacctcca
      181 tttcgattat cagaagagct tccgcattct cgaaaacgcc ggaatcctct acgattcgac
      241 cctcggctac tgggaaaaca tcggcttccg ggcggggata tcctttccct tttatccctt
      301 caacatagag gaaaaccgtc ccttccgagt gctggagata cctctcatcg taatggacac
      361 aaccctccac tccaaaaagg ccatgaacat gaatccgctg gcggccagat taaccctgcg
      421 ccgtttgatc gacatggcgg acaaatacca gtcccatctg tctctgctct ggcacaatgt
      481 aacctttgac cccatagatt ttcccctctg ggctggagtt tactggagta ccctgcgcta
      541 tgccatcagc aaaggcggct ggatcacctc cctgcacgat atctggacag aatggaccta
      601 tttcgactga ccaaggaaaa cccatgttca acaaactaac cagcattctc tggaaagtct
      661 tcttcgtcat catgatgctg ttgttgttca tctttctgct ttttcacctt gtcaccaagc
      721 tgtttttgtc accaagccgt taccgcaggc taacctggca ggtggcaagg gtttggggct
      781 ggtttacggt ggtcagtacc ggcacgcgtg tgaaggtttc cgggcgcgag aacattcctt
      841 tagaagatcc catctgcttc atgggcaatc accaaagtta ttttgacatt ccaaccctcc
      901 ttggttttgc gggctgcccc atgggcttca ttgccaagca ggaattggcc agggtgcctg
      961 tgctcaagca gtggatggtc cagctaccca gtttctttct ggaccgagat aacgcccgcc
     1021 aggccatcaa agtttttcag gcagcggcca aagtgatgaa ggaaggccat cccatggtca
     1081 tttttcctga agggttgcgc gcggagcggg gcaaattggc tgatttacat ctgggcagcc
     1141 tcaagctcgc gcaaatggct ggcgccacca ttgtgccatt cgccttggac ggcacctggc
     1201 gcatgttgga gatcgacggc gatatccacg ccgccagggt gaatttcacc atcctgccgc
     1261 ccgtgagacc gggtgatccc atttacgacg ataaaatcgc cctcgccgct catctgaaaa
     1321 gctccatcga ggcctgcctc acggattgag agacagattt attccaagcc gcttcgccct
     1381 tatggggtat gttgatatcc agtttttagc ctcccgcaca atacccgcat cgagtgcggg
     1441 aatatgacgg gaggcaatag agtggcagaa gatagagggt taagggtgtg tcagcagttc
     1501 agaggcttca ggaactccac gaccaggatg gtggtgtcgt cgtggctttt ctgggtgtcc
     1561 gcaaactcgc caacggcagc caggaccgcc tgaacggttt cttcaggcga agagcctgct
     1621 tctttattca gaaccttttc cagcccgtct gtgccgaata gtttgtcatg tcggtccact
     1681 gcttcggaaa cgccatctgt gaagacgagg attttgtcac cgggcgcaag ttgcagcacg
     1741 tcggaagtga ttttgatatt gtcaaaaaat cccagcgcag tggaatgggt ggtggcgaat
     1801 ttgctgaatc cgcctgccgc cttaagctgg tatattggtg tatggccggc attggaaaac
     1861 acgcaactcc cgctttcaag atcgatgatg cctaggacca tggtcacaaa gtcggatttc
     1921 ggattgtttt ccaccaaaaa ggtgttcagg atgtgcagga tatcctccgg cttgtcttta
     1981 tccgatgaaa ccgtgcgcag caaggttgtc atcatggtca tggtcatggc ggcggcaacg
     2041 ccttttcccg ccacgtcggc aattgcgaag agaaagtgtt tgtcgtcctt gaggaaataa
     2101 tcgtagaggt cgccgccgat ggcaccggct ggttcgagga tgccaaaggc tttcaggttg
     2161 gccggcaact ttgaggcatc atcgtttttg gggataaggt tgcgctggat gatcgaagca
     2221 aaggcaactt cagccatgat gcggttcttt tcctcggtca cttgctgaag gttgtttata
     2281 tggctggtta gcgagctctt cattttttcg aagttctcgg cgataacttg gatttcgtaa
     2341 gtgttcgaat caatctccgg gccagactgc agctcgcctt cccctgccag tttgatggca
     2401 tccaccagtt ctgagagagg ttggttaaga gcactggtgc ggtaccagat cgtgcctgaa
     2461 aagatcagga aagcaagcac gaaagcaatc gtggtgataa tggagagaga gtgcaaatcg
     2521 gcaaaaacct cactgttggg aacctccacc aacaggctcc aatgattgga aggcaggggc
     2581 cgataggcta tccagacatc aggtccaggt ttttcaaaac ggaagcgctc aaagccgctt
     2641 tggccactca cggcgttttt acatatttgg cgcagttcct ggtcgccgta acggttggcg
     2701 aaatcaaacg cggaatagtt catggccagg gaatccctgg gatgggctac gatcatgccg
     2761 ttgcttgcaa tcatgaaggc aaagccggtt tttttcaccc ggatcggctt cacgatgcgg
     2821 tgcaggcttt ccatcggagt gtccaaacgg ataatgcctt gatgcttgcc tttgatggtg
     2881 agcggaaggg agtaagagca tactctttct ttggaaccca ggttgtcgaa ccagggatcg
     2941 ctccaccaag gccttagaga cagccagggt atctgaaacc agtccttgta aaggtaatcg
     3001 tcatcttctg ctgggatgct acgaatctgc tggttttcca aatagaaggt ttgggtgcgt
     3061 ttggtggatg gtttgtcata ggccaggcaa accgagatca gcctggggtt atcgatcagc
     3121 agatggtgga tgtggcgctt aagctgtttc ggggtgtata gatcgtcgtt gatcaccgtc
     3181 ctcagttctt gggcgatggt catgatgttg gagaggttct tgtcgatcat ctgcgcgttt
     3241 gtctctgtga ggttgctaat gctttcttct gcgttgctgc gcatcacttt gtaggccagt
     3301 tggcgggtga tgagaaaggt caccagaaag aggatcaaca tgaagatgaa catgaaagag
     3361 atgagctgtt gccccagata tttggtcttc ttatttgtgt tcatgggggt agaaggtatg
     3421 gtaatcaaca ggtttgtcga ttatgccacg ctcgatcagg atgcgattgg caagatcaaa
     3481 atctttggga tcgagataac caacgcgggc gggattttcc agaatgcggg actttatatg
     3541 ttccagcatc caggcctgat gctcaaggtt tgccggttga tggtttcccc tcaggtaacg
     3601 cagcaccaca gccagggttt cctcaggatt ctggaaggca tagttccagc catctagggt
     3661 ggcggctgtg aaatcccggc actgccgggg gtgtttttca aaaaagtccc tggtagtgta
     3721 gattccatcg tcaacaaggt caaagccgta atccgcaagg tcaaagacca ccaggtcttc
     3781 ttcatccaaa ccggtcatga gcaaacggtg atactcattg tagtacatgg cgttaatcat
     3841 gtccacagca ttttccagca gcaggttgac agaccagtcc atgggtatcg ctcttatgtt
     3901 caggcccagg ctttccagaa agaaaagggt gtggtcgcca ccttcttctc gccagacgcc
     3961 aatctttttc ccatcaagat cttgtatggt tttgattccg gaactctttt tccccaccaa
     4021 caccgtggaa tttttctgcg atatctgagc cattaaaaca gcgggatttt tgggattatg
     4081 atacttgaga gcggtgacca ggttaaagct ggctatgtcg gcggaacctt ccataagcga
     4141 ttggtaaggt ggatgatctc ctcctccggg caggaactcg acatccaagc cccatgcctg
     4201 atagaagcct ttttccttgg ccatgtaagc tccggcgaat tgggcctggt gcagccattt
     4261 cagccggtaa cgcaatttga aaagctctcc gggttgaact tcctccatac tgggtctgtt
     4321 tttcttgctt ctgccagaat caatgcactt gtccccgcaa ctggacaata caaacagcat
     4381 tagaaccacg atcagcctga ccgcgtgacg tatgccacga tgttgaggaa gtttgggttg
     4441 aagcaatggg ttcataatat aaaaacacta aaacctaagc caatgttggg cccataattg
     4501 tttaagcaat gtcatcctag atgatacaat gatcaagtca aggcataata tgccaaaata
     4561 accacaattt agtggaaata tttacacaaa ggcggcagaa gtcttttctt atctactgtt
     4621 tggacttacc ggaagaatag gcatgggcgg caccgaagaa atcccggaaa agggggtgtg
     4681 ccttgtttgg acgggatttg aactcgggat ggaactgcac gccgaggtaa aaaccctttt
     4741 tgggaagttc caccacttcc accaaaagcc cgtcaggtga aaagccgctc aggcacatgc
     4801 ctttggaggt tagcttatcc cgataagcgt tgttgaattc atagcggtgc cgatgccttt
     4861 cgctgatcct ggcacggccg taagcctttc tggccagtga acctggctcc aaattgcagg
     4921 gataagcccc ccgccgcatt gtgcctccgg taagtttctg gtagattgag tcttccatct
     4981 tgtggataac cggatggggt gtgtcggcaa agaattctgt gctgttggcg tctttgaggt
     5041 ggcaaacgtg gcgggcgaat tctatcacag caacctgcat gcccagacag atgcccagga
     5101 aaggtatgtt gcgcgttctg gcatactccg cgatggcgat tttgccttcg attcccttta
     5161 ctccaaatcc gcccgggatc aggattccgt ccacgtcctt gagtattttt gccagggagg
     5221 atggtttgaa tttctgttcg gaattcaccc atttcacctg cagccccatc ctatggtggg
     5281 ctgcggcatg gaacaacgct tcctcgacgc ttttataggc atcctggtgc tttacgtatt
     5341 tgccgcagac ggcgatggtg accttttgtt cccaactggt gctgttttgc aggaacttgt
     5401 tccaggcggt caggcgcaga ggcttgcatt ccaatccgaa gtgacggcat atgatcttgt
     5461 gcagattggc attcttgaag gtgatggggc attcgtaaac gctttttaca tcgatggcgt
     5521 tgattacatt ggcacggggc acgtttgtga agagggcgat tttgtcgtaa atctcgcttt
     5581 caaaaggttt ttccgagcgg cagagcagta tctcaggctg gattccgatc tcacgcagct
     5641 tgtaggcgct gtgttgggag ggtttggtct ttagctcgcc cgctgcgtgg ataaatggaa
     5701 tataggtaag catcacgtag agggtgttct gcaggccaag gtccagtcgc aactggcgca
     5761 cggcttccag aaagggcagg atttcgatgt cgcctacggt tcctccaatt tcggtgatga
     5821 tgatatcgta atcggcatcc agtttttgga tgcggcgctt tatctcatcc gtcacgtgcg
     5881 gaatcacctg caccgtttta cccttatata ttcccttcct ttcgttctgc agcacccttt
     5941 cgtagatttg gccggctgaa cagctcgagt gtcgggttag ggcttcatct acaaaacgtt
     6001 catagtgtcc caggtcgagg tcggtctcag cgccgtcgtc agtcacaaaa acttcgccgt
     6061 gctggaaggg gctcattgtg ccggggtcca cattcagata gggatcaaat ttctgggttg
     6121 ccacgcggta acccattgat ttaagaagca aaccgatgga ggcggtggcg atccccttgc
     6181 caagggaaga tagaacgccg cctatcacga agatgtattt tgccatagat cctcagtaaa
     6241 gcatagctgt ccggggctgg aaatccagcc ccggcagacg gttcatttat attcaggcca
     6301 gctttttaag ctcggccagc gattctttta tagcctgcac cgtgaaggcg aggtcggcgt
     6361 cggtgtgacg gtagcagatg tagccgtgat gataaggctg aaggaaaaca ccccggcgga
     6421 tgagctgggt gtaaaactcc acccgcagct ttttgtaaag gccttcttca tctctcggga
     6481 atgtgatgaa gggcatccat ggcgagccgg aaaaacgggc tggaataccg gattcagcaa
     6541 ccactttttc cacatcttca ccgaactttt tgcctttggc tgccaccaca tccagaatct
     6601 tgtcacgctg aaggatctcg atggtcttca gggccgcaat ctgggcatct gaattgggga
     6661 agaaggtgga ggagaggaag acatccttgg ttaggacatc catgacctcc cgcttgccca
     6721 ccagcgctgc gatcggatag ccgttggcca ttgccttgcc gaaggttgag aggtctggag
     6781 ttactccaaa taattcctgc gcgccaccaa gggaacagcg gaaaccgcta cggatttcgt
     6841 cgaagatcag cacgcagcca tacttgtctg ccagtttgcg gacgccttcc aggtatccag
     6901 gcttgggcat ctgcacttca gctgccaggg gatggccaac cggagtcacg atgatggcgg
     6961 ccatatcgtt ggcattggct ttcaggatgt cttccagttg gtcgaggtca ttgtagtgga
     7021 attcgatgac atcttcgtag aatttctctg ggatgccgcc tttcacttcc acgcaccagt
     7081 cgtgccagcc gtgatagccg cagcgtgcaa ctttcacctt gccggtgtaa gctcgggcaa
     7141 cgcggatgga gatggtggtt gcgtcgctgc cggtcttcac tatcgcggcc atttcgcaac
     7201 agggaaccac ttcctgcagc ttctgcacca gcttgttttg gatggattgg gtgagtgaga
     7261 aacaaaagcc tttgttcttg atttgctcga tcaccgcgtt gtcgatttct tcctcgcggt
     7321 agccaatgat aatggggccg taagcgcaaa ggtaatctat gtattcgttg ccgtccacat
     7381 cgcggatgcg cccgcccttg ccttcttcaa agaagatggg gtattcgccc tcaacgaagt
     7441 tgtagggtct gcgtatgccg gccacagcgc cgggcacgag ggttttggcc tcggcataca
     7501 gagccatgct cttgtccagt ttcagtttgt ctgccatgtt agttactcct ttcccagagg
     7561 gttgctatgc ccattccacc acctatgcag agggcggcaa ggcctttatt gaggttgcgt
     7621 ttgcccattt catgcagcag ggtaacgatg atgcgggctc cgctggagcc gatgggatgt
     7681 cccaaagcaa tggctccgcc gttgacgtta actttgtcca tatccagttt gcccacgcct
     7741 tccatttcca agcctttgaa aacagccagg g
//
LOCUS       DAOOWR010000033         7737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9420_length_7737_cov_3.944025, whole genome shotgun sequence.
ACCESSION   DAOOWR010000033 DAOOWR010000000
VERSION     DAOOWR010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9420_length_7737_cov_3.944025"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLB85_01630"
     CDS             complement(<1..440)
                     /locus_tag="PLB85_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU72056.1"
                     /translation="MRTSTAIALALVVLILNAGITLVLINQKGLNTVSEPASKIIVSH
                     NQRHNEITAAVQEVEPAVVSVNVTKVQYVRRFPSFSFFDFFGDIPMQRQVESIGSGVI
                     YDSSGLIVTNAHVVQGASEITVVLPDKREFPAVLVGIDDVHDVA"
     gene            complement(449..1207)
                     /locus_tag="PLB85_01635"
     CDS             complement(449..1207)
                     /locus_tag="PLB85_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOU72057.1"
                     /translation="MKDQDQYLSVLKFPAFVSLSLLIISALYICLVYFILQDQVTEPH
                     LVRGLLIALLVVIVFMIFTWVSTLMSTHIRIKPRWMSQLNKWMLNHVFYPMACALGTI
                     TFSSKNVLTESFLNFNNEIVLTDQKNVRNSNILVLLPHCLQKDDCTVRITTDIINCEE
                     CGGCDIATIKKLIERQQVEAAVATGGSLARKLIQDKNPNVIVAVACHRDLLDGLRDAW
                     RYPIYAVLNERPNGPCHETTVSIASIEFAIQIFK"
     gene            complement(1204..2178)
                     /gene="fmt"
                     /locus_tag="PLB85_01640"
     CDS             complement(1204..2178)
                     /gene="fmt"
                     /locus_tag="PLB85_01640"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOU72058.1"
                     /translation="MRLIFAGSSTFGLPSLQLLERQDVPLLVISPPDKAAGRNLRLQP
                     CPVADYARREGLDLFQPPDVNAPESLLRIREFAPDLLITASYGGMIRRELRNLPRYGA
                     VNLHPSLLPKYRGATPIQSSLLNGDTITGVSIFRLNARMDAGPILMQHQVLIIKTDDF
                     GSLHNRLANLSALMLQNLMPQLESGSCEAIAQDDSAASYTRKLGKEDLQLDWYKPANQ
                     VLNRIRAFSPQPGAQAWLREKPLKILAAQLVQEPASGEPGTVAALIRNTGVEVNCGVQ
                     RLLLTRVQAAGKKEMDAWPWQLGARLSIGESLSRPPTFKPSNPLQEEE"
     gene            complement(2196..2750)
                     /gene="def"
                     /locus_tag="PLB85_01645"
     CDS             complement(2196..2750)
                     /gene="def"
                     /locus_tag="PLB85_01645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU72059.1"
                     /translation="MSRSPKLLDIHVYGDDVLRLRAAEVETIDDELLQFAKDLTHTMY
                     KRDGVGLAAPQTGVSKRVIVVDPHWSKEGAKREPLVMINPEIESSDGETEMEEGCISV
                     PGIYARVQRASAISVSFTDLSGERRLMKLSGYPAVVVQHEHDHLNGILFVDRLGTIAK
                     LKVKRKLKELERKAVNGVNLRGED"
     gene            complement(2750..3106)
                     /gene="yajC"
                     /locus_tag="PLB85_01650"
     CDS             complement(2750..3106)
                     /gene="yajC"
                     /locus_tag="PLB85_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU72060.1"
                     /translation="MLYTLLMTGAGGSSGTAPAQQTNPLMSLLPIIIMFALLYFLMIM
                     PQQKKQKELQKMLDNIKVNDKVITTSGIYGRVVTIKPDKNSVVIEIDETNHVRVEFQR
                     GAIATVVTGENSTAQS"
     gene            complement(3116..3604)
                     /locus_tag="PLB85_01655"
     CDS             complement(3116..3604)
                     /locus_tag="PLB85_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOU72061.1"
                     /translation="MNSRRLSPTTFLASLLGVGFIPFAPGTFGTLFAAGVYLLLPKSL
                     FSGQGWWWLGGALLLVSGAAVWLSGRAEKKLGQDAPAVVIDEFCGYFVAVLLLPKSLL
                     LAIYAFVLFRVFDITKPFPINVSQRLRGGWGIVVDDLIAGVYANLVIQLIRILKPSFF
                     GQ"
     gene            complement(3601..4863)
                     /locus_tag="PLB85_01660"
     CDS             complement(3601..4863)
                     /locus_tag="PLB85_01660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU72062.1"
                     /translation="MKKTLLTLLAAVLMLSLSAELVDRIVAKVGPDIVLLSDVYKQMF
                     QMQTAGYPAESINEEAALRQIVEQKVILQKAESMDLKIDNKQLEKYAKKELEKTKEKY
                     PSEQAFAADLARENLTETELLDLYITQIKESYLSQQLIEIYVKTKVKVTEEEMLSFYE
                     TSKDSLAVKPITWKTGAIIHEIKPSKASEEAKLAEMKAIQDRLNQGADFASLAMEVSD
                     CPSKERGGDLGFFSRGMMVKPFEEAAFALNAGQVSPIVQTEFGYHLIKVEEKRDNEVR
                     ARHILKILSPTRQDSLDAFQMMESVRERFNSGEESFANLATLYSDEKESAANGGIIGE
                     LARDEFPELYAPQIIATEVGKMTPVLENEGVLLLFARLEEMPTRVFSYDEVKPVLENY
                     LMQEKFNTTYNEWISDLISKSFVRIIPR"
     gene            complement(4906..5721)
                     /locus_tag="PLB85_01665"
     CDS             complement(4906..5721)
                     /locus_tag="PLB85_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72063.1"
                     /translation="MRKILPLLILLLALFSACDREEATTEKLTPLAEVNGDVLTLEGF
                     RSTFTDEQWNNLSSEQKKQEIENWVNLTLLAQEAEAQKLDGERAVKQRIDYAAKKIKA
                     NALISKRLASIAIGEEELFNYYRIHQSEFQSKLMEYDIQRILCSDAARAASLLQRLKR
                     DGYDFDLAVAEHSQEALKNNKGRMGFVTASGSDSLFWRAAHELPANTPGMASINGQTY
                     ILRHVQQREGTQDANFAEYQAEIRSILLKERKQQVYQELIRELKMKTPEIYYY"
     gene            complement(5725..6900)
                     /locus_tag="PLB85_01670"
     CDS             complement(5725..6900)
                     /locus_tag="PLB85_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72064.1"
                     /translation="MMVGCIKKGNPTGNNWSDVRPITVTEPLDMIAGYSFAGSAEITG
                     TESSLLCGNYDGIESVAFMRFTGLPNPGTFHIPSAYQDSTFLSLTLTRRSTPQGNPIN
                     LTIYKLNQAWAADSTDLIQDANLIEISNIEMTIPDSISTMGTEIKIPIPIDEIETWQT
                     SSDSLGMTLAIRTMSGFWMETMSLETGRGPQLNFLYRLDEATNTDDSRYEQRPTRDSY
                     RVDSDEAPLLTNRWIVGNINPSRIYMDFAIDNSWFKDMRGNVMDSLSRKRTTINQARL
                     ILYVKDNPYYTGSRTYKLRADRMNDSLDITQPVEIEDSRLATGIPTEQLVRGDSLVVD
                     ITPIIQAYSSGDKIPYGIVIRSLQEMINYGKIEFWHFEDAPADKRPKLRLTYTPPFL"
     gene            complement(7208..>7737)
                     /locus_tag="PLB85_01675"
     CDS             complement(7208..>7737)
                     /locus_tag="PLB85_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU72065.1"
                     /translation="PDFETRVAILRKKAEPENIDLPDEVFSFIAESISSSVRALEGSL
                     IRILAYASYNKLNPANLDTETVRNILSDMISESRREISLDTITQQVCLAYNITIAQIV
                     DKTRRQQIAYPRQIAMYLANLLIPQLSLKEIAEYFKRKDHTTVLHAKKMIDNRFREDI
                     NFRSHIEQLIKNIKA"
BASE COUNT         1666 a         1933 c         2156 g         1982 t
ORIGIN      
        1 ttggcgacgt cgtgaacatc gtcgatgccc accaggacgg ctggaaactc acgcttgtcg
       61 ggcagcacga cggtgatttc ggaggcgccc tgcaccacgt gggcgttggt gacgatgagg
      121 ccgctggaat cgtaaatgac tccgctgccg atgctttcaa cctgcctttg catgggaatg
      181 tcgccaaaga aatcgaagaa actgaagctc ggaaagcggc gcacatactg gacttttgtg
      241 acgttgacgc tcaccacagc gggctccact tcctgcactg cggcggtgat ctcgttgtgg
      301 cgctggttgt gagagacaat tatctttgag gctggttcgg acactgtatt caagcccttt
      361 tggttgatca ggacgagggt gatccctgcg ttcaggatca gcaccaccaa ggccaaagct
      421 atcgctgttg aagttctcat ggttgcacct acttgaatat ttgaatggcg aattcaatac
      481 tggcaatgct gacagtggtt tcgtggcaag ggccgttggg acgctcgttc agaacagcgt
      541 aaatcggata ccgccaggcg tcgcgcaggc cgtccagcag gtcgcggtgg caagccacgg
      601 caacaatgac gttgggattt ttgtcctgaa tgagcttgcg ggccagcgaa ccgccggttg
      661 ccactgctgc ctcaacttgt tgccgctcaa tcagtttctt gatggtggca atgtcacagc
      721 cgccgcattc ctcacagttg atgatgtccg tggtgatgcg cacggtgcaa tcatcttttt
      781 gcaggcaatg cggcagcaac accaggatgt tggaattgcg gacgtttttt tggtcggtga
      841 gcacgatttc gttgttgaaa ttgagaaatg attccgtcag cacgttcttc gaggaaaaag
      901 tgatggtgcc cagagcgcag gccatcggat aaaacacgtg gttaagcatc catttgttta
      961 gctggctcat ccagcgcggt ttgatgcgga tatgggttga catcagggtg ctcacccaag
     1021 tgaagatcat aaagacgatc accaccagta gagcaattag gaggccgcgc accagatgcg
     1081 gctccgtaac ttggtcctgc agaataaaat agaccaagca gatatagagg gcgctgatga
     1141 tcagcagact gaggctgaca aaagcgggga atttcaacac ggacagatat tggtcctgat
     1201 ctttcattct tcctcctgaa gagggtttga gggtttaaaa gttgggggtc ggcttaaact
     1261 ttcgccgatg gacagacgcg ccccgagctg ccagggccag gcgtccatct cctttttccc
     1321 ggctgcctga acccgggtga gcagcaaacg ctggacgccg caattcactt cgacgccagt
     1381 attccttatc agggctgcca ccgtgccagg ctcgccgctg gcgggttcct gaaccagttg
     1441 ggcagccagg atcttgagag gtttttctcg cagccaggcc tgggccccag gctggggtga
     1501 aaaagcccgg atgcggttga gaacttggtt cgcgggctta taccagtcca gctgaaggtc
     1561 ttctttgccc agtttgcggg tgtagctagc cgcggaatca tcttgggcaa ttgcttcaca
     1621 agatccgctt tccaactggg gcatgaggtt ttgcagcatc agagctgaga gattggccag
     1681 cctattgtgc agactgccaa aatcgtctgt tttaatgatg agcacttggt gctgcatcag
     1741 gattggtccc gcgtccattc gggcattaag gcgaaaaatg ctgacgcctg taatggtgtc
     1801 accattcagc aaagagcttt ggatgggcgt cgcgcctcga tatttaggca gcagagaagg
     1861 atgcaggttc accgctccgt agcggggcag atttctcagt tcgcgcctga tcataccgcc
     1921 ataggaggcc gtgatcaata ggtcgggagc gaattcccgg atccggagca gactttccgg
     1981 agcatttacg tccggcggct gaaacaggtc caggccctcc cggcgggcgt aatcagccac
     2041 gggacagggc tggaggcgca gattgcgtcc ggcggccttg tcgggcgggc tgatgacgag
     2101 caggggaaca tcttgccgct caagcagttg cagggatggt aacccgaagg ttgagctgcc
     2161 ggcgaagatc aagcgcatta catcgttttc ccggttcagt cctcaccgcg aaggttaacg
     2221 ccgttcacag ccttgcgttc gagctctttg agcttgcgct tcacctttaa cttggctatg
     2281 gtgccgaggc ggtcaacgaa caggatgccg ttcaggtggt cgtgttcatg ctgaaccacc
     2341 acggcaggat aacccgacag cttcatcagg cggcgttctc cgctcaggtc ggtgaaagaa
     2401 acgctgatag cagacgcccg ctgcacccgg gcataaatgc ccggaacgct gatgcagcct
     2461 tcctccattt ctgtttcgcc gtcgctggat tcgatctcgg gatttatcat caccagcggt
     2521 tcgcgtttgg ctccttcctt gctccagtgc gggtccacca caattacgcg tttgcttaca
     2581 cccgtctggg gtgccgcaag ccccaccccg tcacgcttgt acatggtgtg ggtgaggtct
     2641 ttggcgaact gcaacagctc gtcatcgatg gtttcaactt ccgctgccct caggcgcaga
     2701 acgtcgtcgc cataaacgtg gatatccaaa agcttgggac tgcgggacat caggattggg
     2761 cggtgctgtt ttccccggtg acaaccgtgg ctatggcccc gcgctgaaat tccacgcgca
     2821 cgtggttggt ttcgtcaatt tcgatcacga cggagttttt atccggtttg atggttacaa
     2881 cgcgtccgta gattcctgat gtggtgatga ccttgtcgtt taccttgatg ttgtccagca
     2941 tcttctgcag ttctttctgc ttcttctgtt gaggcatgat catgaggaag tagagcaggg
     3001 cgaacatgat gatgatgggc aataaggaca tcaggggatt ggtttgctgt gcaggggcag
     3061 tgccggaact tccgccggct ccagtcatta acagtgtata gagcatttat ttctcctatt
     3121 ggccaaagaa gcttggcttc agtattctta tcagttggat cacaaggttc gcgtaaacgc
     3181 cggcgatgag gtcatctacc acgatacccc atccgccgcg caggcgctgc gaaacgttga
     3241 tgggaaaagg tttggtgata tcgaaaaccc gaaacaggac gaaggcgtag atcgccagca
     3301 gaagtgattt tggcagtaac agcacggcta cgaaatagcc gcaaaactca tcgataacga
     3361 cagccggagc gtcctgcccc agtttctttt cggccctgcc gctcaaccag acagccgccc
     3421 cggaaacgag cagcagtgct ccacccagcc accaccagcc ctgtccgctg aagaggcttt
     3481 tgggcaacag tagatatacc cccgcggcaa aaagagtgcc aaaggtgccg ggcgcgaagg
     3541 ggatgaaacc cacacccaaa agcgaggcca gaaaagtggt cgggctcagg cgccgggaat
     3601 tcatcgcggt atgattcgca caaatgattt ggagatcagg tcgctgatcc attcattgta
     3661 ggtggtgttg aatttctctt gcatgaggta gttttccaaa acgggtttca cctcatcata
     3721 actgaagacg cgggtgggca tttcttccag ccgggcgaaa agcagtagca ccccctcgtt
     3781 ttccagcaca ggtgtcattt taccaacttc ggtggcaatg atttgtgggg catagagttc
     3841 cggaaattca tcccgggcca gttctccgat gatgccgcca ttggcagcgg attccttttc
     3901 gtctgaatag agggtggcca agttggcaaa actttcctcc ccggagttaa acctttcacg
     3961 tacgctttcc atcatttgaa aggcatccag gctgtcttgc cgggtgggag agaggatttt
     4021 caggatgtgt ctggcccgca cctcgttgtc acgtttttcc tccaccttga taaggtgata
     4081 gccaaactcg gtttgcacga tgggcgaaac ctgacccgcg ttgagcgcga aagcagcttc
     4141 ttcaaagggt tttaccatca ttccccggct gaagaaacca aggtctccac cccgctcttt
     4201 actggggcaa tcactcactt ccatagccag ggaggcaaaa tccgccccct ggttaaggcg
     4261 gtcctgaata gctttcatct cagccagctt cgcttcctcg gaggctttgc tgggcttgat
     4321 ctcgtggatg atggcgccgg tcttccatgt aatcggctta accgccaggc tgtccttgct
     4381 ggtttcgtaa aaagacagca tttcctcttc ggtgaccttc actttggttt tcacgtagat
     4441 ttcgatcaac tgctgtgaga ggtagctttc cttaatttgg gtgatataga ggtccaaaag
     4501 ctctgtttcg gtcaggtttt cacgggcgag atcggcggca aatgcctgct cggaaggata
     4561 tttttccttg gttttttcga gctctttttt ggcgtatttc tccaactgct tgttatcgat
     4621 cttaaggtcc atggactcgg ctttttgaag gatcaccttc tgttcaacga tctggcgcaa
     4681 ggcggcttct tcgttgatgg actcagccgg gtaacccgct gtttgcatct ggaacatctg
     4741 tttgtaaaca tcgctcaata aaacgatgtc tgggccaacc ttggctacta ttcggtcaac
     4801 caactccgcg gacaggctaa gcatcagcac agcggccagc agagtgagca gggtcttttt
     4861 catatctttc cttgttggga tgtgttggtg tcggttaaga ctggttcagt aataatagat
     4921 ttccggagtc ttcatcttaa gttcccggat aagttcctgg taaacttgct gttttctttc
     4981 ttttagcagg atggaacgga tctcagcctg atattcggcg aaattggcgt cctgggttcc
     5041 ctcccgctgc tggacatgac gtaggatgta ggtttggccg ttgattgagg ccatgccggg
     5101 tgtgttggcc ggtaattcat gggcggcacg ccaaaatagc gaatccgaac cggaggcagt
     5161 tacaaaaccc attcttccct tattgttttt cagagcttcc tgggaatgct cagccacggc
     5221 cagatcaaag tcgtatccat cccttttcag cctttgcagg agggaagcgg ctctggccgc
     5281 atccgaacag aggatgcgct ggatgtcata ttccatcaac ttgctctgaa actcgctctg
     5341 gtggatgcgg tagtaattga aaagctcctc ctcgccaatg gcgatgctgg ccaggcgctt
     5401 ggagatcagg gcgttggctt tgattttctt ggcggcataa tctatacgct gtttcacagc
     5461 ccgctctcca tccagttttt gggcttcggc ttcctgggcc agaagggtca ggttaaccca
     5521 gttttcaatt tcctgctttt tctgctcaga gctgagattg ttccactgtt cgtcagtaaa
     5581 tgtggagcgg aatccttcca aggtgagcac gtctccgttc acctcggcca atggtgtaag
     5641 tttttcagtg gtcgcttcct ccctgtcgca ggcagaaaac aatgccagca gcaggatcag
     5701 gagcggaagg attttgcgca tggctcaaag gaacggcgga gtgtaggtta ggcgcagctt
     5761 ggggcgtttg tcggcaggag cgtcctcaaa gtgccagaat tcgattttgc cgtagtttat
     5821 catttcctgc aacgaacgga tcacgatgcc atagggaatt ttatcaccgg agctgtaagc
     5881 ctggatgatc ggtgtgatgt ccaccaccag gctgtccccg cgcacaagct gctctgtcgg
     5941 tatccctgta gccaggcggg aatcttcgat ttccaccggc tgggtaatat ccagggaatc
     6001 gttcatccgg tcagccctca gcttgtaggt gcggctgcct gtatagtaag gattgtcttt
     6061 cacatacaaa atcagcctgg cctgattgat ggtggtccgc ttgcggctca aactgtccat
     6121 cacgttcccc cgcatgtcct tgaaccatga attgtcgatg gcgaaatcca tatagatgcg
     6181 ggaagggtta atattgccta cgatccagcg gtttgtgagt agaggcgcct cgtcggaatc
     6241 aactctgtag ctgtcccggg tgggtcgctg ttcatatctg ctgtcatcgg tgttagttgc
     6301 ttcgtcaagg cggtaaagga agttaagctg aggccctctt ccggtttcca gagacatcgt
     6361 ctccatccag aaaccgctca tggtcctgat ggccagggtc attcccagtg aatcagagga
     6421 agtttgccag gtttctatct catctatagg aatgggtatc ttgatttcgg tgcccatggt
     6481 gctgatactg tcgggaatgg tcatttcgat attggatatc tctatcaggt tggcatcctg
     6541 tatcaagtcg gtgctgtcgg ccgcccaagc ctggttcagc ttatagatgg tcaggttgat
     6601 gggattgccc tggggagtgg agcggcgcgt gagggtcagc gaaagaaagg tggaatcctg
     6661 ataggcggag ggaatgtgga aagttccggg gttcggaagc ccggtgaaac gcatgaaagc
     6721 cacggattcg atcccatcat aatttccgca cagcagcgaa gactcggtgc cagtgatttc
     6781 agcgcttccg gcaaaactgt agccggctat catgtcaagc ggttccgtaa cagttatcgg
     6841 cctcacatct gaccagttgt tgccggtggg atttcccttc ttgatacacc cgaccatcat
     6901 caaggccaaa atgaccaata gtagaggtat aagtttgttc ttcaacatca tattctttat
     6961 acctaattaa aagttccttt ctcaaaaagt agtgttagta ggcatcgtgg ataagtggat
     7021 tttcggtcaa cctttttttt aagtggctat ccataaagcc ttggcgaagc ctctcaaatt
     7081 gtggataact tgtgggtaag catgttcttc cgcacggagt tttccacaca ggagcaagca
     7141 tttatgttta aaaccaggtt atccacactt atccacataa actatccact tatccacaat
     7201 cccctgctca ggccttgata tttttgataa gctgttcgat atgcgagcgg aagttgatat
     7261 cctcccggaa gcggttatcg atcatttttt tggcgtgcag cacggtggtg tggtccttgc
     7321 gcttgaaata ttcggcaatt tctttcaatg aaagctgggg aatgagcaga ttggcaagat
     7381 acatggcgat ttggcgggga taggcgattt gctggcggcg ggttttgtca actatctgtg
     7441 cgatggtaat gttataagcc aggcagacct gctgggtaat ggtgtccagc gatatttccc
     7501 tgcggctctc ggaaatcatg tcgctgagga tgttgcgcac tgtctcggtg tccagattcg
     7561 cggggttgag tttgttgtag gaagcgtagg ccaggatgcg gatcagcgaa ccctcgaggg
     7621 cccgcacgct ggaagagatc gattcggcga taaaggaaaa aacctcgtcg gggagatcga
     7681 tgttctctgg ctcggctttt ttgcgcagga tggccaccct ggtttcaaaa tccggat
//
LOCUS       DAOOWR010000034         7634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9599_length_7634_cov_3.715662, whole genome shotgun sequence.
ACCESSION   DAOOWR010000034 DAOOWR010000000
VERSION     DAOOWR010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9599_length_7634_cov_3.715662"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1812)
                     /gene="ligA"
                     /locus_tag="PLB85_01680"
                     /pseudo
     CDS             complement(1..1812)
                     /gene="ligA"
                     /locus_tag="PLB85_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
     gene            complement(1809..2606)
                     /locus_tag="PLB85_01685"
     CDS             complement(1809..2606)
                     /locus_tag="PLB85_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOU72066.1"
                     /translation="MNYRVSVLQYEPRLLDLSGNLKQLKQMLSGLETDLVVLPELCAT
                     GYVFTSLEEVASVSEEVPSGAAFGTFSALASENRFSIVYGFAERSGNIFYNSAALINP
                     DGSYHIYRKTHLFDREKLFFTPGDTGLHVFPAKDGVQVGMMVCFDWTFPESARTLALK
                     GAQIICHPSNLVLPWCQQAMTTRSLENRVFSITSNRTGDESNGGVSLHFTGQSQILGT
                     KGEILTRMRVKETGVRTCEIDPELAFDKQVSPLNHAFSDRRPAFYDL"
     gene            2850..3809
                     /gene="trxB"
                     /locus_tag="PLB85_01690"
     CDS             2850..3809
                     /gene="trxB"
                     /locus_tag="PLB85_01690"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU72067.1"
                     /translation="MYYDVMIIGGGPAGLSAAIYAARGGLKTGLFEKGIIGGQINVTD
                     EVENYPGFPEPISGFELTDKMRQQAERFQAHIIDEEVTAIGLEGLCKIVETQENKYRA
                     KALIVCTGAHPRLLNVPGEERFTGRGVSYCATCDGALYRNKVVAVVGGGDSAIEEGLF
                     LTRFASKVIVIHRRDELRAQKILQERAFKNPKIEFIWDSVVREIHGNGKIEKLEVVNV
                     KTQQASFVPVDGVFIYVGILPNNELLESMLEQDSGGFLLTDDHMHTNVPGIYAAGDIR
                     STVLRQVVTATSDGAVAAWSAEKWIIENFDHVEGLGEAPSADK"
     gene            3796..6399
                     /gene="alaS"
                     /locus_tag="PLB85_01695"
     CDS             3796..6399
                     /gene="alaS"
                     /locus_tag="PLB85_01695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU72068.1"
                     /translation="MLTNKKIRQTFIDFFLERGHIFVPSSPVVPEDDPTLLFANAGMN
                     QFKNVFLGLKEAEAKRAVNSQKCIRAGGKHNDLEEVGRDGYHHTFFEMLGNWSFGDYY
                     KKEAILWAWELLTEVWKLPKDKLYATVHDSDQEAFEIWKSVTDIDPAHISYQGDKDNF
                     WEMGDTGPCGPCSEIHIDRGPAHCSRQGEEGHICEICGDCGRYIELWNLVFIQYNREA
                     DRSLTPLKDRFVDTGAGFERLVQVLQRKDSNYETDLFLPIIERVVELSGVPYTPETGT
                     SHRVIADHVRCLCFALADGGFPSNEGRGYVLRRILRRAARHGRLLGFAEAFMFHLVDT
                     VISIMGHHFPELAGKEAYIRMVIKAEEERFTAALDKGLEHLDQICAKLEGDLISGKDA
                     FMLYDTYGFPLDLTLILASEKNLRVDTDGFEEEMEAQRARARKSSKFAFEASSEDWIE
                     LEASSPTVFLGYEKNNATAQIQRYRISEDGLLHLQLDRSPFYAESGGQLADTGRIFNS
                     GFEMQVSQVRKNDDHFIHSGTLISGAVNSDPVTAEIDLPRRQDIMRNHTATHLLHKAL
                     RTVLGEHVQQKGSLVGPDYLRFDFTHMQALSPVEINSIESIVNAAIRDNLPVSVELKN
                     IAEARSEGAIALFGEKYSEQVRVVSVSDYSKELCGGTHVSASGEIGLFKIVSESSSAA
                     GIRRIEALTGRAAEQFVLDLQRKLAQLADKLHVPEKMLDSKIEALQNRVQELEQQLKQ
                     LDAQKADAEAAELIRRIRDLGDFKLLSQKIDADAAKLRELGDSLKDRAPDAISLLFAT
                     AREKVTILCVVGEKLRPKFHAGNIVKAVSAKLEGKGGGRPDSAMGGGNRPDLLDEMIK
                     DLPQIIGSFSQ"
     gene            complement(6750..7556)
                     /locus_tag="PLB85_01700"
     CDS             complement(6750..7556)
                     /locus_tag="PLB85_01700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU72069.1"
                     /translation="MEYDPLKDRAASLIRVWPAARVFFYRVLDLLLLRQRYVKLALRQ
                     YLPIDRGFRYYDAGAGFCQYSWHVLKNYPAAKVFANDLKRDYLEDFSRYAKKSFPGRF
                     SWQQADLQTFTPRNSYGLATAIDILEHIQDDEAVLRNFNVALADGGILIISTPSDTDE
                     AAKFTSEHVRPGYNKAELEDKLRACGFTILESIYSYGTWGSLAWRLLLKHPLKLIARS
                     KLILLALPTYYLLVFPLAELLMRLDLKSSNPTGTGIIIVAQKRLSSEPDR"
BASE COUNT         1726 a         2069 c         2144 g         1695 t
ORIGIN      
        1 gcggtcgtag ttatccaggc tgcccgtgag caaaaaggtc ttgccggcca gggtttccga
       61 ttcgcgccgc agctcgtaac tgaaacgcag gccctgagcg cgcagcttct ccaccaattt
      121 cagattgagg gggttaccaa accaggcttt cagggacagg gcgatcttgc ttcccacctc
      181 tggaacctgg gccagggttt cttccccggc aatcagcagt tcatccaccg tgccaaaata
      241 ctcggcgaga tggcgtgccg tgacggaacc cacataacgg attccgaggg caaagagcac
      301 ccggtcgaaa ttcctggctt tgctggcttc gatggagttc ctgaggtttt cggctgattt
      361 ctcgccaaag cggtcaagct gtgccaaggc ttcataatca agccgataga tgtcagcgag
      421 gccggcgatc atgttttgct ccagtaatct ggcgatcagg ctggtgccca ggccgctgat
      481 gtccattgca tcgcgggagg cgaagtgctc gatgcggcgc tgcaattggg cggggcagga
      541 ggcgttaggg caatagctga tggcgccttc ctcatcccgt tcaagctgac tggagcagac
      601 ggggcagttg gctgggaatc tcaccggaac ggcgtttggt tcgcgcttgg ctgggatggc
      661 ggtgaggatc ttgggtataa tttcgccgga tttgataatg cgcacggtgt cgttcagatg
      721 caggtccagc cgcttaattt catcctcgtt gtgcagggtg gcacgtgaga cggtgctgcc
      781 ggagatgtag accggctcca ggatggccac cggggtcacc gctccggtgc gtcccacctg
      841 atattgcacc tccaacaagc gggtttcctt ttcctccggc ttgaatttga aggccaccgc
      901 ccatttggga cttttggcgg tgtagcccaa tcgcttttgc agagcaagat catccacttt
      961 caccaccact ccgtcgatct cgtagggcag gctgtagcgt tcattttacc agtggtcgca
     1021 gaattcccta acctcgttgt aactggcgca aaccctgaac tggcctgacg tggggaatcc
     1081 ctgatccgct agccaggcaa gaagctcgga ctgcctttgc agaggcaaat ccgggctgtg
     1141 gccaatgctg taaaagatgg cgttcaggtg gcgcttcttc gcctcttcag ggtttttcag
     1201 cttgatggag ccggcagcgg cgttgcgggg attggcaaaa gccttttctt cattggcttg
     1261 tcgctcttcg ttgagtttca gaaaatctgc cacgggaatg tatatctcac cgagaatttc
     1321 gatgggtgag aggtgcgcaa ttttcttcgg gatggagctt aggtttaaga tgtttggagt
     1381 taccacctcg ccggtgatgc cgtcgccgcg cgttgtggca tggccaagtt cgccgttttc
     1441 ataataaagg ttgatggaaa agccgtcgat cttaagctca gcggtaaggg tggggaagaa
     1501 accaagttcg gaagcgagtt tggcgcacca ctgttccagt tcctccgtgg aatagctgtt
     1561 gtcaaggctg tacatcctct gcaagtgggg gatcgtctcc gctcccggcc tcaggtcgct
     1621 gcccaccttg tccaggggag attcaccgcg ctcatctgcc tccataaggg cttccagttc
     1681 tttgagctga cgcgccagct ggtcatattc aaaatcgctt atggagggat tggccagctc
     1741 gtaatagagc aggttgtggc gttctatctc agcccgcaac ctttggattt cccgtttcaa
     1801 ctcatcattc ataaatcata aaaggccgga cgccggtctg aaaaagcgtg gttcaggggt
     1861 gacacctgtt tgtcaaaagc gagttcggga tcgatttcgc aggtccgcac cccggtttct
     1921 ttcactctca tccgggttag gatctctccc ttggtgccca ggatctggct ctggccggtg
     1981 aaatggaggc tcaccccgcc gttgctttca tctccggtgc gattggaagt gattgaaaac
     2041 acccggtttt ccaaacttct ggtggtcatt gcctgctggc accagggcag aaccaggttt
     2101 gagggatggc agatgatctg agcacctttc agggccaggg ttcgcgccga ttcggggaag
     2161 gtccagtcga agcaaaccat catccccacc tgcactccat ccttggctgg aaaaacgtgc
     2221 aatccggtgt cgccgggggt aaaaaagagc ttctcgcggt cgaagagatg tgttttcctg
     2281 taaatatggt aacttccgtc gggatttatc agggcagcag agttgtagaa tatattcccg
     2341 ctgcgttcgg caaagccgta aacgatgctg aaccggtttt ccgaagccag agcgctgaaa
     2401 gttccgaaag ccgctccgga gggcacttcc tcgctaacgg aggccacttc ctccagagag
     2461 gtgaaaacat aaccggtggc gcaaagctca ggcagcacca caaggtctgt ttccagaccg
     2521 ctcaacatct gtttcaactg cttcagattg ccgctcaggt caagcagacg gggctcatat
     2581 tgcagcacgg aaaccctata attcattcca gcccccttgt ccgaaaagct tccagacctt
     2641 gttttcttcc agccatcaga gccattctac gcaaaacagg caaactcatt ttgtcactcc
     2701 caaatttcta ctttatacca taaaagctac ttccgccaat tcggtcaagt tcttttcaga
     2761 ttctggccat ccagataatt tccttgacgg aggaaggcgg ttcaaaagac acgttaattt
     2821 ctaagaattt cctgatattg aggtagataa tgtattacga cgtgatgata atcggtgggg
     2881 gccccgccgg gctgagcgca gctatctacg ctgcccgtgg cggcctgaaa accggtctgt
     2941 ttgaaaaagg gataatcggc ggccagatca acgtgaccga cgaagtggag aactatcccg
     3001 gttttccgga accgatttca ggctttgagc tcaccgataa aatgcgccag caggccgaac
     3061 gcttccaggc ccatataatt gacgaagagg ttaccgccat tggtctggag gggctttgca
     3121 agatagtaga aacccaagag aataaatacc gcgccaaggc tttgatcgtt tgcaccggcg
     3181 cccatcccag actgctcaac gtgccggggg aagagcgttt caccggccgc ggcgtatcct
     3241 actgtgctac ctgcgacggc gcgctctacc gaaacaaagt ggtggcagtg gtcggcggag
     3301 gagattccgc catcgaagag gggcttttcc taacccgctt tgcctccaaa gtgatcgtga
     3361 tccaccgccg cgatgaactc cgtgcccaga agatccttca ggagcgcgct ttcaagaacc
     3421 ccaagataga attcatctgg gattctgttg ttagggaaat ccatggcaac ggaaaaatcg
     3481 agaagctgga agtagtgaac gtcaagaccc aacaggcttc cttcgtcccg gttgatggag
     3541 tcttcatcta tgtgggcatc ctgcctaaca acgaactgct ggaatccatg ctggaacagg
     3601 acagcggagg ctttttgctc actgacgacc acatgcacac aaacgttccc ggcatttatg
     3661 cagcggggga tatccgcagt accgttctgc gccaggtggt caccgccacc agcgacggcg
     3721 ctgtggcagc ctggagcgcg gaaaagtgga tcattgaaaa cttcgatcac gtcgaaggac
     3781 tgggagaagc tccaagtgct gacaaataaa aagatccgcc agaccttcat cgactttttc
     3841 ctcgagcgtg gacacatctt tgtgccatcc tcgccggttg tgccggaaga cgatcccacc
     3901 ctgctttttg ccaacgccgg catgaaccag ttcaaaaacg tcttcctcgg cttgaaggaa
     3961 gcggaagcga agcgtgccgt gaacagccag aagtgcatcc gcgccggcgg caagcacaac
     4021 gaccttgagg aagtgggtag ggatggctat caccacacct tttttgaaat gctgggcaac
     4081 tggtctttcg gcgactatta caaaaaagag gccatcctct gggcctggga actgcttacc
     4141 gaagtctgga agcttcccaa ggacaagctt tacgccaccg tgcacgactc tgaccaggag
     4201 gccttcgaga tatggaaatc tgtcactgat atcgatcccg cccacatttc ctaccagggc
     4261 gacaaggaca atttctggga aatgggggat accggcccct gcggaccctg cagcgaaatc
     4321 cacattgacc gcggtccggc ccactgttcc cgtcagggcg aggaaggcca catttgtgaa
     4381 atctgtggtg actgtggccg ctacatagag ctctggaacc tcgttttcat ccagtacaac
     4441 cgtgaggcgg accgctcact caccccgctc aaagacaggt ttgtggatac cggagctggt
     4501 ttcgagcgcc ttgtgcaggt gctgcagaga aaggacagca attacgagac cgacctcttc
     4561 ctaccaatca tagagcgcgt ggtagagctc agcggagtgc cctacacacc tgaaaccggc
     4621 acatcacatc gcgtgatcgc agaccacgtt cgctgcctct gcttcgccct tgccgacggc
     4681 ggtttcccct ccaacgaggg ccggggatac gttctgcgcc gaatcctcag aagggcagcc
     4741 cggcatgggc gcctgctggg ttttgccgaa gccttcatgt tccatctggt ggataccgta
     4801 atttccatca tgggccacca ttttcccgag ctggcgggca aggaagccta tatccgcatg
     4861 gtgatcaagg ccgaggaaga acgcttcacc gcagccctgg acaagggttt ggagcatctc
     4921 gaccagattt gcgccaaact cgaaggtgat ctcatctccg gcaaagacgc cttcatgctt
     4981 tacgacactt acggctttcc gcttgacctc accctgatct tggcttccga aaagaatctc
     5041 agggttgaca ccgacggctt cgaggaagag atggaagccc agcgcgcccg ggcccgcaag
     5101 agttccaaat ttgccttcga ggccagttcc gaagactgga tcgaactgga agcttcatct
     5161 ccgacagtgt ttttgggata tgagaagaac aacgccaccg cgcaaatcca acgttacaga
     5221 ataagcgaag acggcctgct gcatttgcag ttggaccgca gcccctttta cgcggaatcc
     5281 ggaggacagt tggcggacac agggcgcatc ttcaattccg gctttgagat gcaggtcagc
     5341 caggtgagga agaatgacga tcacttcatc catagcggca ccctcatcag cggggccgtt
     5401 aatagcgacc ccgtcacagc ggagatcgat ctgccccggc gccaggacat catgcgtaac
     5461 cacacagcca cccatctgct ccacaaggcc ttgcgcaccg ttttgggcga acacgtgcag
     5521 cagaaaggct cgctggttgg tcctgactat ctgcgtttcg atttcaccca catgcaggcc
     5581 ctgtccccag tcgagatcaa ttccatcgaa agcatcgtca atgccgctat ccgcgacaac
     5641 ctcccggttt ctgtggaact taaaaacatc gctgaagccc gctcggaagg cgccatcgcc
     5701 ctgttcggtg aaaaatactc tgagcaggtt cgcgtggtga gcgtatcgga ctattccaaa
     5761 gagctttgcg gcggcaccca cgtttccgcc agcggtgaga tagggctctt caagatcgtt
     5821 tccgaaagct cctccgccgc ggggataagg cgcatcgaag ccctcacagg acgcgccgct
     5881 gagcagtttg tgctggacct tcagcgcaag ctggcccagt tggcagataa gctccacgtt
     5941 cccgaaaaga tgctggattc caagattgaa gctctgcaaa accgggtgca ggaactggaa
     6001 cagcagctaa aacaactgga cgcccaaaaa gctgatgccg aggctgctga actcatacgc
     6061 aggataaggg acctggggga tttcaagctg ctcagccaga aaatcgatgc cgacgccgcc
     6121 aagctccgcg aactgggcga ttcactgaaa gaccgtgccc ccgacgctat ctcactactc
     6181 ttcgcaaccg cgcgggaaaa ggtgacgatc ctctgcgttg tgggggagaa gctgcgaccc
     6241 aaattccatg ccggaaacat cgtcaaggca gtttccgcca agctggaagg caaaggcggc
     6301 ggccggcccg attccgccat gggcggcggc aatcggcctg acctgctcga tgaaatgatt
     6361 aaggatctgc cgcagatcat cggctccttt tcccaatagg aatccctcct ttcccaatct
     6421 ggttggtgat ccgcgaggaa taaccccaag ctgaattttg cgcggtgctt ctgccgtgtt
     6481 tctgaggctc tctgtcgatt cgggtgagta acactctggg gcggaataca atagcagggt
     6541 tgcgaagtag ccgggaaacc cactggtaag cgaagcgatg tcatcaggcc attaaggcct
     6601 tttttaagat cattttcctg cctcccgcac aatcgccgca tttgatgcgg ggatatggcg
     6661 aggggcgttg gagcgcgatg gtcgagaggg ttaaggacct gactgagggg aagtatcggt
     6721 gggtaattgt ttctatcggt ctcgaggttt tacctgtccg gttcagaaga gagacgtttc
     6781 tgagccacga tgatgattcc ggttccggtt gggttggaag atttaaggtc cagtcgcata
     6841 aggagttccg ccaggggaaa gacgagcagg taatacgttg gcagggccaa caggatgagc
     6901 ttgcttcgag caatcaactt tagtggatgc ttcagtagga ggcgccaggc caggctgccc
     6961 caggtgccgt agctgtaaat gctttcgagg atggtgaagc cgcaggcgcg gagtttgtct
     7021 tccagctctg ccttgttgta gccggggcga acgtgctcag aggtgaattt ggccgcctca
     7081 tcggtatccg agggagttga gatgatcagg attccgccat ccgccaaagc tacattaaaa
     7141 ttgcgtagca cggcctcatc gtcctgaata tgttccaaaa tgtcgatcgc ggttgccagg
     7201 ccataggaat tgcgcggtgt gaaggtctgc aaatccgcct gctgccagga aaagcggccg
     7261 gggaagctct ttttagcgta gcgggagaaa tcctccaggt aatcgcgttt gagatcgttg
     7321 gcaaagactt tggcggcagg atagttcttc aacacatgcc aggaatactg gcagaaaccg
     7381 gctccggcat cgtagtagcg gaatccacgg tcgatgggaa ggtattggcg cagggcgagt
     7441 ttcacatagc gctggcgcag cagcagcaga tccagaacgc ggtagaaaaa cacgcgcgcg
     7501 gcaggccaaa ccctgatcaa actcgcggca cggtctttga gtggatcgta ttccatcaaa
     7561 actccaatag ttcgcttttc cattcccagc agactgggaa cttggcataa aataatcatt
     7621 gacgattttt gggg
//
LOCUS       DAOOWR010000035         7539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9766_length_7539_cov_4.475548, whole genome shotgun sequence.
ACCESSION   DAOOWR010000035 DAOOWR010000000
VERSION     DAOOWR010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9766_length_7539_cov_4.475548"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /gene="recA"
                     /locus_tag="PLB85_01705"
     CDS             <1..1004
                     /gene="recA"
                     /locus_tag="PLB85_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU72070.1"
                     /translation="LEKKYGVGTLMRLGDKPQQTVEVIPTGAFNLDIALGIGGIPRGR
                     ITEIYGTEASGKTTLALHIAAEAQKLDGVVAFIDAEHALDTAYAKNLGVQTEELLLSQ
                     PDGGEQALEICETLVRSSAVDLVIVDSVAALVPKQEIEGDMGDSHVGLQARLMSQALR
                     KLTAIVSKSNTAVIFINQTRMKIGTMPFMNPETTSGGVALKFYSSVRLEVRFAGAIKA
                     GGAEAEVIGAKTRVKIVKNKLAPPFKTVVFPIIFGEGISQLDIIMDMAVDAGIIKKSG
                     SWFAYKDIKLGQGAEKTKQYLKETPELLDEIEDAVKQNINPDKIFDKTDAAEDSSEE"
     gene            976..1584
                     /locus_tag="PLB85_01710"
     CDS             976..1584
                     /locus_tag="PLB85_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOU72071.1"
                     /translation="MRLRTVPKNRYTVRVELDGICRGTLPTRVLLPLWPDNFEGEIER
                     DKAQELLALLEKQAFSTLLDYLAKAEHSEFQCRNLLKRKEFEPRIIDAAIQRCREQNF
                     LSDARFADVLIRSYIARKASKRAIIAKLREQRIPGEIWTELLEELYPREQASENISEL
                     LAKYCSTHRGLPRQKLRGKAFGYLFRKGFELEEIQSAWEELA"
     gene            complement(1632..2279)
                     /gene="nth"
                     /locus_tag="PLB85_01715"
     CDS             complement(1632..2279)
                     /gene="nth"
                     /locus_tag="PLB85_01715"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU72072.1"
                     /translation="MPKFNIDAVMKALAEHFDRVKTPIVDLIQVQTKDPFKVLVATIL
                     SARTKDETTAKASAALFAEVSGPDDLENISADRLNSLIRQVGFHNEKTKHLKQLPAVL
                     KERFGGKVPSEIDDLLQLPGVGRKTANLVRAVAFSLPAICVDVHVHRISNRWGYVSTK
                     TPFETEMALRKKLPEKYWLTYNSHVVAFGQNLCSPRKPRCSECPVFKYCARVGVA"
     gene            2376..3041
                     /locus_tag="PLB85_01720"
     CDS             2376..3041
                     /locus_tag="PLB85_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU72073.1"
                     /translation="MCGRFAQVIKHDQLQKMEKELRLTHLAEQEELNFNVAPTQTVMA
                     VLSQDAGRYPGYFRWGLIPSWSRELPKFNLINARADTITSKPSFRNGLQRRRCLIPAN
                     GFYEWRVSDKQPFFIHAVNDDLIWMAGIYDAWSGPDGSFIPSLAIITTDANDAMAQIH
                     HRMPVLLFGGDRLAWLNHRQSDPLDFVPLLKPAPEGELALRPVSKFVNRVGNNSEECW
                     AAV"
     gene            complement(3045..4334)
                     /locus_tag="PLB85_01725"
     CDS             complement(3045..4334)
                     /locus_tag="PLB85_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOU72074.1"
                     /translation="MNPGIAMFAALIIFAVTYIFIITEWINKMLASLVGGFLLIVTGI
                     LTQGEAFKAVDWNVIFFLIGMMLVISVLKKTGVFMYVAIKTAKLVRGHPVAIMMLMFA
                     ITAFFSAFLDSVTAVMILVPVVMLICHELEITPVPFIITMAVASNMGGASTMVGDPPN
                     VILGSAIGKTFLDFVTNLAPAAIASVIFSMGLIYLFYHKKLHVSLTNRAKLMSYNEDK
                     LIKDKRTLVISLSVLSLMLLALALDGVLHIGTASISMTAALILMIISNRRNMEPVMAK
                     EIDWVTLFFFIGLFIIVEGLVKTGFINMFANKVISATGGNPRSTSMAILWLSGIFSAF
                     VDNVPFVATMIPVLKQISQVINNPALMDPIWWCLSLGTCLGGNGTLIGASANIVSVGI
                     AKQNGFHISFWDFTKISVVFTLCSLVICTVYILLRYF"
     gene            complement(4331..5236)
                     /locus_tag="PLB85_01730"
     CDS             complement(4331..5236)
                     /locus_tag="PLB85_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU72075.1"
                     /translation="MFLADFLDARAIAFEQRVLPREQVYQEIIQRICAQRSHNTPKCG
                     QPMLDAILQREREAPMAYPSGIAIPHIRVEGLADTLIGMTFLQNPLDYDGIKVNWVVL
                     IFTDKSSSKIYLNIVAALLKLSRDEEAMKSLHGLHDGHDVIHWLKQAKVEVGTDVTVA
                     DIMIRNPFCVGPDDVLRLANSLINEHKVSMLPVTDKEGTYLGELNILDILKVGVPDYL
                     MMMEDLKFLKSYEPLENLFEKEDEITAGEIMQKDNKVLEPDDSIMEAVYLMLKEGRRY
                     FCVVEDGKLLGVVTAMDVFRKVVKA"
     gene            complement(5648..6313)
                     /gene="phoU"
                     /locus_tag="PLB85_01735"
     CDS             complement(5648..6313)
                     /gene="phoU"
                     /locus_tag="PLB85_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOU72076.1"
                     /translation="MLNSKIKELKQLLLTEAGWVEKMISLALDGLYSHNNDLLNIVIG
                     YEDKVNQMDLEIENKCISAIALYQPEAKDLRKIVMILKINNDLERLGDRAVNIAESAM
                     HLMGKPVVSQVSELAQMRDSAFAMLKASLDAFATENAEACSAICEADNRVDELNRAVY
                     HRLVDLIKQDTPLTESCLHLLRVARNLERIADLSTNVAENTVYLAVGKVIKHQQGEED
                     NPE"
     gene            complement(6327..7085)
                     /gene="pstB"
                     /locus_tag="PLB85_01740"
     CDS             complement(6327..7085)
                     /gene="pstB"
                     /locus_tag="PLB85_01740"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOU72077.1"
                     /translation="MVDIQLQSLDLNLWFGKNHVLHDVSLPVYKHQVTAMIGPSGCGK
                     STLLRCFNRMNDLIPGTRIEGRALLGDADIYAPKADVSKLRSRIGMVFQKPNPFPKSI
                     FNNVAFGLQIQGGFSKAEISNRVEESLKAAWLWEEVKDRLSSSAMSLSGGQQQRLCIA
                     RALANRPDVLMLDEPTSALDPQSTAKIEELCLELKHNVTILIVTHNVAQAGRISDQTA
                     FMYLGELLEFGPTERVFTVPADKRTEAYLTGVFG"
     gene            complement(7079..>7539)
                     /locus_tag="PLB85_01745"
     CDS             complement(7079..>7539)
                     /locus_tag="PLB85_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU72078.1"
                     /translation="SLLSGGLTLAILALPVIINNTEQAIRSVPEDFREASLALGATTR
                     QTVSRVLLPTALPSILTGAIISIGRVAGETAPIMFTAATFYSRQLPKGLGDEVMALPY
                     HIYALMTEGTHPTEQVPIAYGTSLVLLLLVISISAAAIWIRSSIRRKRQW"
BASE COUNT         1730 a         2013 c         2117 g         1679 t
ORIGIN      
        1 agcttgagaa aaaatacggc gtgggcacgc tgatgcgcct gggcgacaag cctcagcaga
       61 cagttgaggt gatccccacc ggggctttca atctggatat cgccctcggc attggcggca
      121 tcccgcgcgg ccgcatcacc gaaatctacg gcaccgaggc cagcggaaag accaccctcg
      181 cgctgcatat cgccgcggaa gcgcaaaaac tggacggcgt ggtggccttc atcgacgccg
      241 aacacgccct ggatacagct tacgccaaga atctgggcgt gcagacggag gaactgttgc
      301 tctcccagcc ggacggcggg gaacaggccc tggaaatctg cgagaccctg gtgcgcagtt
      361 ccgctgtgga cttggtgatc gtggacagcg tggcggcctt ggttccaaaa caggaaatcg
      421 aaggcgacat gggcgacagc cacgttggcc tgcaagcccg cctgatgagc caggcgctgc
      481 gcaaactcac ggcgattgtc tccaaatcca ataccgcggt catcttcatc aaccagaccc
      541 gcatgaaaat cggcaccatg cccttcatga acccggaaac aacttccggc ggcgtggcgc
      601 tgaagttcta ctcctccgtg cgcctggaag tgcgttttgc tggagcgatc aaagccggcg
      661 gagctgaagc cgaggtaatc ggagccaaga cccgcgttaa aattgtgaaa aacaagctcg
      721 ccccgccctt caagacagtg gttttcccca tcatcttcgg cgagggaatc tcccaactgg
      781 acatcatcat ggatatggcg gtggatgccg gcatcatcaa gaagagcggc tcctggttcg
      841 cctacaagga cattaagctg gggcagggcg cggaaaaaac caagcaatac ctgaaagaga
      901 cgcctgaact gctggacgaa atcgaagacg ccgtgaaaca gaacatcaat ccggacaaga
      961 tctttgacaa aacagatgcg gctgaggaca gttccgaaga atagatacac ggtccgcgtc
     1021 gaactggacg gaatttgcag gggcaccttg ccaacgcggg tgctccttcc tctttggccg
     1081 gataatttcg agggcgaaat cgagcgggat aaagctcagg aactgctagc cttgctcgaa
     1141 aaacaggcct tttccaccct gctggactat ctggccaagg ctgaacacag cgaattccag
     1201 tgcaggaacc ttttgaaaag gaaggagttc gagccccgaa tcattgacgc cgcgatacag
     1261 cgctgccggg agcagaactt tctgagcgac gcccgcttcg cggatgtgct gatccgttcc
     1321 tacatcgcac gcaaagccag caaacgcgcc atcatcgcca aactacggga acaacgcatc
     1381 cccggagaga tttggacaga acttctggaa gagctctatc ctcgcgaaca ggccagcgaa
     1441 aacatcagcg aactgctagc caaatactgc tccacacacc gcggcttgcc ccgccagaag
     1501 ctgaggggaa aagccttcgg ctatcttttc cgcaagggtt ttgagctcga ggaaatccaa
     1561 agcgcctggg aggaactggc ttaagctctg ccaaaggcct ggcgtcaagc cttactgcta
     1621 catcatttaa gctaagccac cccgacccgg gcacaatatt taaagactgg gcattctgaa
     1681 cagcgcggtt tgcggggcga gcagaggttt tggccaaaag ccaccacgtg ggaattgtag
     1741 gttagccagt atttttcagg cagttttttc cgcagggcca tctcagtttc gaagggggtt
     1801 ttggtgctca catagcccca gcggttgctg atcctgtgta catgcacgtc cacacagatg
     1861 gcaggcaggg aaaatgctac cgcccgaacc aggttggcgg ttttgcgacc cacgccggga
     1921 agctggagca aatcgtcgat ctcggaggga accttgccgc cgaagcgttc tttcagcacg
     1981 gcgggaagct gtttgaggtg tttggtcttc tcgttgtgga agcccacctg ccggatgagg
     2041 gagttgagcc tgtcagcgct gatattttcc agatcgtcgg gaccgctcac ttccgcgaac
     2101 agggctgcgg aggccttggc ggtggtctcg tccttggttc tggcgctgag gatggtcgcc
     2161 accagcacct tgaaggggtc tttagtctgc acctggatga ggtccacgat gggggtcttc
     2221 accctgtcaa agtgttcggc gagcgccttc atcacggcat cgatattgaa tttgggcatg
     2281 gtctttcctt ttttggctgt gaatccgata aacgtcaaaa aatatgttgc aggatttccc
     2341 gggctgccga atctgtcaac cagaaggagt ttagcatgtg cggaagattt gcccaggtga
     2401 tcaaacatga ccaactgcaa aagatggaga aagaactgcg gctgacccat ctggcggagc
     2461 aggaggagct gaatttcaac gtggcgccca cccagacggt gatggccgtt ctgagccagg
     2521 acgcaggacg ctatccggga tattttcgct ggggcctgat tccctcctgg agccgggaac
     2581 tgccaaaatt taacctgatc aacgcgcggg cggataccat cacatccaag ccctcgttcc
     2641 gcaacgggtt acagcgccgg cgctgcctca ttccggccaa tggtttctat gaatggcgcg
     2701 ttagcgacaa gcagcctttc ttcatccacg ccgtgaacga cgatttgatt tggatggcag
     2761 gcatttacga cgcctggagc ggcccggacg gaagcttcat tccctccctg gccattatca
     2821 ccacggacgc caatgacgcc atggcacaaa tccatcacag gatgccggtg ctgctcttcg
     2881 gcggggacag gctggcctgg ctgaaccacc ggcagagcga tccgctggac ttcgttccgc
     2941 tgctcaaacc agccccggaa ggcgaactgg ccctgcgtcc ggtgagcaaa ttcgtgaacc
     3001 gggtgggcaa caactcagag gagtgctggg ctgccgtctg aagcttagaa atagcggagc
     3061 aggatgtaaa cagtgcaaat caccagagag cagagggtga aaaccacgct gatcttggtg
     3121 aaatcccaaa acgagatgtg aaagccattc tgcttggcta tccccacaga gacaatattg
     3181 gcagaggcgc cgatcagggt tccgtttccg cccaagcagg tgccgagcga aaggcaccac
     3241 cagatggggt ccatcaatgc ggggttgttt ataacctgac ttatctgttt gagcacgggg
     3301 atcatggtgg ccacgaaagg aacgttatcc acaaaagcag aaaatatccc tgaaagccag
     3361 aggatggcca ttgaggtgga gcggggattt cctcccgtgg cgctgatcac tttgttggcg
     3421 aacatattga tgaagccagt tttgaccagt ccttccacga tgatgaagag cccgatgaag
     3481 aaaaagaggg tcacccaatc tatttccttt gccatcaccg gttccatatt cctgcggttg
     3541 ctgatgatca tcaggatcaa cgccgcggtc atggaaatgc tggccgtgcc gatgtgcaga
     3601 actccgtcca gggccagcgc caggagcatt aggctcagca cggataggga aatcaccagc
     3661 gtccgcttgt ccttgataag cttgtcttca ttgtagctca tcagcttggc ccggttggtg
     3721 agacttacgt gaagtttttt gtggtaaaaa agatagataa gtcccatgct gaagatcacg
     3781 gaggcgatgg cagccggggc gaggttggtc acaaaatcca gaaaagtctt gccgatggcg
     3841 cttcccagaa tcacgttggg tgggtcaccc accatggtcg aggcaccgcc catatttgag
     3901 gccacagcca tggttatgat gaagggcacg ggggttatct ccagctcatg gcagatgagc
     3961 atcaccaccg gaaccaggat catcacagcg gtaacgctat ccagaaaagc cgagaaaaaa
     4021 gcagtgatgg caaacatcag catcatgatg gccacagggt gccccctcac cagtttggcg
     4081 gtcttgatag ccacatacat gaagaccccg gttttcttga gcacggatat caccagcatc
     4141 atgccgataa gaaagaatat gacgttccag tcaacagcct tgaaagcctc gccctgggtc
     4201 aggattccgg taacgatcag gaggaatcca ccgacgagag aggccagcat cttgttgatc
     4261 cactctgtga tgatgaagat gtaggttacg gcaaagatga tcagagccgc aaacatggca
     4321 ataccggggt tcatgctttt accaccttcc tgaagacgtc catagctgtc accacgccca
     4381 gcagtttgcc atcctcaacc acgcagaaat acctgcggcc ttccttgagc atgaggtaaa
     4441 ccgcttccat gatggagtcg tccggctcca gcaccttgtt gtccttttgc atgatttcgc
     4501 cagcggtgat ctcatcttcc ttttcaaaga ggttttccag gggttcgtat gatttgagga
     4561 atttcaagtc ttccatcatc atcagataat ccggaactcc caccttgaga atgtccagga
     4621 tgttgagttc gcccagatag gtgccctcct tgtcggtgac gggcaacatt gaaaccttgt
     4681 gttcattgat gaggctgttt gccagtctga gaacgtcgtc aggcccaacg cagaagggat
     4741 tgcgaatcat gatatcggcg acggtcacat ccgtcccgac ctcgactttc gcctgcttta
     4801 gccagtggat cacgtcatgc ccgtcatgca ggccatgcag gcttttcatt gcctcttcat
     4861 ccctggaaag cttcagcagg gcggccacga tgttcagata gatcttggag gacgatttgt
     4921 ccgtgaagat gagcaccacc cagttcacct ttatgccgtc ataatccagg ggattttgca
     4981 gaaaggtcat gccgatcagg gtgtccgcga gtccttcaac ccggatgtgg gggatggcaa
     5041 tcccgcttgg ataggccatt ggtgcttccc gctcgcgttg caggatcgcg tccagcattg
     5101 gctgtccgca tttgggggtg ttgtggcttc tttgggcgca aatcctctgg attatctcct
     5161 gatagacctg ctcccggggc aggacacgct gctcgaacgc gatggcccgg gcatcgagga
     5221 aatccgctaa aaacattatt ttacctctga aaagtgattt aatatgtgca gttttttgcc
     5281 gcgggcaaat ccagtcaatg ttttttatgg ctctctttca aaccaggtgg gaaaagccca
     5341 aggcactgct ttccggtcga gctttcgcgc ccacctggcc tgaaaaagaa ctatgaagaa
     5401 atgcttaccc actgtcaagt cctgtcggct acacccttag cgccttatat catcctcctc
     5461 ccatgcctcc cgtataatac tcgcatcaag tgcgggcatt atgtgggagg cacaaacggg
     5521 acacaggaaa aggccttaac ggccaatcag catagattcg ccgttcagcg gggattgatt
     5581 agtgtcgccc tccgggctgg aaacaccggg ttttatccac tcggtttgaa agggagcttt
     5641 tttgtgctta ttcggggttg tcttcctcac cctgctggtg cttgatcact tttccgacgg
     5701 ccagatagac ggtgttctcg gccacattgg tggaaagatc cgcgatgcgt tccaggtttc
     5761 tggcgacgcg cagcagatgc aggcaggatt ctgtgagggg cgtgtcctgc ttaatcaggt
     5821 ccaccaaccg atggtaaacg gcgcggttga gctcgtcaac ccgattgtcg gcttcgcaaa
     5881 tggcgctgca ggcttccgca ttctccgtgg caaaggcgtc caggctggct tttagcatgg
     5941 caaaagccga atcccgcatc tgcgcgagtt ctgaaacttg ggataccact ggttttccca
     6001 tcagatgcat cgcgctttcg gcgatgttca ccgcccggtc gccgagccgt tccaagtcgt
     6061 tgttgatctt gaggatcatc acgattttgc gcaggtcttt cgcttccggc tgatagaggg
     6121 cgatggctga gatgcatttg ttctctatct ccagatccat ctgattcact ttatcctcat
     6181 acccgatcac gatgtttaaa agatcgttat tatgtgagta caggccgtcg agggccaggg
     6241 agatcatttt ttccacccag cctgcctcgg tgagcagcag ctgctttaat tccttaatct
     6301 ttgagtttag cattctttct cctggcttaa ccgaagacac cggtgaggta ggcttcggtg
     6361 cgtttgtccg cgggtaccgt gaaaacccgt tcggtggggc cgaattccaa cagttcgccc
     6421 aggtacatga aagcggtctg atccgaaatc cggccggctt gggcaacgtt gtgtgtaact
     6481 ataagtatag tcacattgtg tttaagttcc aagcacaatt cttcgatctt ggcggtggac
     6541 tggggatcaa gcgccgaagt gggttcatcc agcatcaaca cgtcgggacg gttggcaagg
     6601 gcccgggcaa tgcacagcct ctgttgctgg cctccggaaa ggctcatcgc ggaggaggat
     6661 agccggtctt tcacctcttc ccaaagccaa gcggctttca ggctttcctc aaccctgttg
     6721 ctaatctctg ccttgctgaa tccgccctgg atctgcaagc caaaggccac gttgttgaag
     6781 atgcttttgg gaaagggatt cggtttttga aaaaccatac cgatgcgcga ccgaagcttg
     6841 gaaacatcgg ctttgggagc atagatgtcc gcgtctccga gcaaggccct gccctcgatg
     6901 cgggtgccgg ggatgaggtc gttcatgcgg ttgaaacacc tcaacagggt tgattttccg
     6961 cagccggagg gaccgatcat ggctgttacc tgatgtttgt aaactggcag gctgacgtcg
     7021 tgcagaacgt ggttttttcc gaaccagaga ttgaggtcca aactttgtaa ttgtatatct
     7081 accattgtct tttccttctg atggacgaac ggatccagat ggccgccgcg ctgatggaga
     7141 ttaccagcaa aagaagcacc agggaggttc cgtaagctat gggaacctgc tccgtgggat
     7201 gggtgccttc ggtcatcagg gcgtagatgt gataaggcag ggccatcact tcgtcgccca
     7261 ggcctttcgg cagttgacgg gagtaaaaag tggcggctgt gaacatgatc ggcgctgttt
     7321 caccggctac gcggccgatg ctgatgatgg cgccggttag aatacttgga agcgcggtgg
     7381 gcaaaagcac gcgggaaacc gtttgccggg tggtggcacc caaagccagc gaggcttcgc
     7441 gaaaatcctc tggaacgcta cgtatggcct gttccgtgtt gtttatgatc accggcaggg
     7501 ccaggatggc caaagtgagt ccaccggaaa gcagggaaa
//
LOCUS       DAOOWR010000036         7467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9909_length_7467_cov_3.682947, whole genome shotgun sequence.
ACCESSION   DAOOWR010000036 DAOOWR010000000
VERSION     DAOOWR010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9909_length_7467_cov_3.682947"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLB85_01750"
     CDS             complement(<1..965)
                     /locus_tag="PLB85_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU72079.1"
                     /translation="MSDKVRETSSKAALKEMREEYLRMLEESFQTTAEFKKGDVVEAP
                     IISIGDNHMILSLGGKFDAYAENGEFADEKGELPYAVGDTLKGYIVDSNDQGFVIGKS
                     LTKQYVDKQSLLDAFERKIPVAGKVYSVTKGGFNVDVLGARAFCPISQMSMRGGDANI
                     DYIGKTLDFMVIECSENCRRVVVSRRQLLEAEENERKAEALKKLEIGSVVTGKVMRMT
                     SFGAFVDLGGIEGLMHVSEISWQHVVKPQDVLKQGQEVEVKILDIKGEKLSLSMKALM
                     ENPFEQLVKEIKEGDTINCRILRLHNFGAFAEIKPGVEGLIPVSE"
     gene            1671..2936
                     /locus_tag="PLB85_01755"
     CDS             1671..2936
                     /locus_tag="PLB85_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOU72080.1"
                     /translation="MSSLHHQVYQSYIQSMHRFCQYPVLAEGLRQAELGIRVKLLAIG
                     KAAWKMAAVSVNALAERNIKAEGLVLTKPGLSHGGIPGLAILSGGHPLPNENSLASSS
                     RIAAWLKKLSAKDDLVILLSGGSSALFELLPEGFDLADLATEHKLLLHSGKDIAEINR
                     GRTGLSLVKGGRALELTKARRIKVFAVSDVEGNDPQVLGSGPFTPAEPGEKTADGWRY
                     KLGTKQIEYNIVADNQGFRELFAKDLREKGFRVLVEKSFQGCPLPKLANQIKEILRRT
                     RSPRYRLRPPFIKILGGESPLKVSGEGLGGRCSHLALLLVRYLAKEEETALFCFATDG
                     CDNVSSSGGAWSDSNTLAQLKAAGINLAAAIRKCDSYTALKSVNHLLPAPLLATNVND
                     IHVLSVGYSLQSPCPSRDWEAMDIFDELV"
     gene            2961..5342
                     /locus_tag="PLB85_01760"
     CDS             2961..5342
                     /locus_tag="PLB85_01760"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOU72081.1"
                     /translation="MALLLTLLLALGWTWLLAQEETELPASVAPADSLLKTPAPEDSL
                     FYAADSLAYNHALEQIRLYGNTSITYQTFEISSDSLLVDLKNNLAFSQSNTVMRDGDQ
                     IILGDDVAYDIDSQTGSLSGGISRMEKSYYTGEEIRKISSDAYDVDNGSFTTCENEEP
                     DFWFTAKKLRIFRGDKIVGKPVVAYVNHLPVFYFPFIVVPLQSGRKPGFLIPQPGYNT
                     IDGKFFRDIAWYYPYKDYADLILSLDLYERTGWRAELQLDYLRRYLLNGSLDAAFQRR
                     SGGSQTFNDWSLQATHHQELGNRATFDANVDFVSNKRIWEGSSDIDESLAQTVTSSLS
                     YRQPLLGSYLNIGASYTEDLINSRGTVSLPSASFALPTRPLYELFYKPERSSDAWWSN
                     LNYSYNLRLDHSGSLYSSDRLFMDYVWNNRFAPGDSVWHNFGIKHQLGLSYNWKAFGW
                     LGLQHGARYNEAWFDRDKNGNTLVRGNDYNAYTNANFNIYGIRNFKKGWLKSLRHIVT
                     PNAGISFNPDFSANSRFYSFGGVGLLNSARSANLNLSLDQKWQIKFGKDDRKINDVFS
                     LNSRSSANLLNPSAKPFQTISHTAAFRPGSFSLGNLRFPGTKTKLEGLSLAYSAQFSL
                     SHSPYGVSWDDWKISNQYFSQGLTLTGSAPYQDYFPKAKNRIFDPYQKADSLQFFAQT
                     LSEAESSNTWRIALSHDLSATADLFDPISQSLRLDSSFRLTKNWSVAYGSYFNLKTGE
                     QLSQSIRVSRDLHCWKLELSYTRRNDYWEYKVALFNLALPDALRFQTSDSKSF"
     gene            5323..5910
                     /locus_tag="PLB85_01765"
     CDS             5323..5910
                     /locus_tag="PLB85_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU72082.1"
                     /translation="MIAKAFRRAVFLDRDGCVSPDEFGYISNPAGYQLYPWTGEALRL
                     LRSLGYLLILVTNQSGIARGYFDIPALNRVHDRLQELLAAENVKLDGIYFSPYFADGS
                     VAPYNIEHEDRKPGLGMFRKARRAHRINPGQSWMIGDRQTDITFGKNAGLSSILLLTG
                     NGAREMLSGLQDWPRQPDYIAENLLTAAKLIELLR"
     gene            5910..6728
                     /locus_tag="PLB85_01770"
     CDS             5910..6728
                     /locus_tag="PLB85_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU72083.1"
                     /translation="MKTRGSFSQRKFQSLSPSAQVKALDKLASVLEKSLAGREPASEL
                     TSHVLECVSWMAEPVPENVLRLSQDLASAAATREAQLAITRFKSSLGQSFKDNQIPLK
                     RGDGPRFADLNTLERAGKVILILDNLRSAFNVGSIFRSAECLGLCKIWLCGVTATPND
                     HALRKTARGTASRIDWSHFEETAEAVKQARAEGYRVYALEISAQAESVFAEDFQLPLA
                     LVLGNESLGISNEILSLCDRTVCLPVQGWKNSLNVAVACAVCAYQIVFGCPSSH"
     gene            complement(7037..>7467)
                     /locus_tag="PLB85_01775"
     CDS             complement(7037..>7467)
                     /locus_tag="PLB85_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOU72084.1"
                     /translation="FQVGTKVPVKIIREDKVKTLYIQLDNFPEDTVAETEEAAAPDSG
                     AGISVEATDSAYAQRNNIKASKGVIVSAIESNSPASKAGIQVGFVVLKVGNTEVNSPE
                     EYSAAMNAAIDKMRKDERNTIILYVQDRNKTEQFVVLRFN"
BASE COUNT         1659 a         2261 c         1947 g         1600 t
ORIGIN      
        1 atttccgaaa cggggatgag gccttccacg ccgggtttga tctcggcaaa ggcgccaaaa
       61 ttgtggaggc gaaggatgcg gcagttgatg gtgtcgcctt ccttgatttc cttaaccagt
      121 tgttcaaagg ggttttccat cagggctttc atcgaaaggg agagcttttc gcccttgatg
      181 tccaggattt tcacttccac ttcctggccc tgctttagga cgtcctgggg cttcacgacg
      241 tgctgccagg aaatctcgga gacgtgcatc aggccttcga tgccgcccag atcgacaaag
      301 gcgccgaagc tagtcatgcg catcaccttg ccggttacga cggagccgat ctcaagcttt
      361 ttgagggctt cagccttcct ctcgttttcc tcggcttcca aaagctggcg acgggaaacc
      421 accacgcggc ggcaattttc ggaacactcg atcaccatga aatccagggt tttgccgatg
      481 tagtcaatat tcgcgtcacc gccccgcatg ctcatctggg aaatggggca gaaagcgcgg
      541 gcgccgagga catcgacgtt gaacccgccc ttggtgactg agtaaacttt gccggcaacc
      601 gggattttgc gctcgaaagc gtcgagtagt gactgtttgt ccacatattg cttggtaagg
      661 cttttgccga tgacgaatcc ctggtcgttg ctgtcaacga tgtagccttt gagggtgtcg
      721 ccgacggcgt agggcagttc gcccttttcg tcggcaaatt cgccgttttc ggcataggcg
      781 tcgaacttgc cgcccaggct caggatcatg tgattgtcac cgatactgat gatgggggct
      841 tcaacgacgt cgcctttttt gaattccgct gtggtttgga aagattcttc gagcatgcga
      901 agatactctt cccgcatttc tttcaaagca gctttgctgc tggtttcacg caccttgtct
      961 gacataacta tctcctaagg acatttcaga aagaccacga tttccgtgac cccttttttg
     1021 taaaggattt ttattgcggc aaaaggacag ggcaaggata aacattctga cctgatgaac
     1081 atttctccca tgaagaaaag ggagttcaaa gagtgcgtgg agaggttgat agacagggag
     1141 gccgtatcag tggatgttca aaataaacca aacagagcac ccaatcttta tatactggaa
     1201 ggcagcatca tggaaacata gaaagcgccg caagatacaa gttaaccaat actgctccag
     1261 catggtgggg aggcttctag catccacaca attacgaaaa ttcactaccc cgattattag
     1321 ggtgtgaatt ttgtgaattt tcatcacctc ctggtgtttt ccaactacgc tggagtcgag
     1381 tgaggaacaa gcttctacta cagcaattcc acttgctctc attttcatag ggataaactg
     1441 attaaacgga ttgacggatt cttggttctc ttttcaaatc gagctattga tcgatggaga
     1501 cattgaccaa ccactcagcc tggcgtacag cgaacacagt gagcatggac gccagagacg
     1561 gtggagtgac ggggacagac aggcggcttg gcggagtggc tgaaaaatga acttgactaa
     1621 atcctttcct gcttttctaa tgattttaaa tacattatta ggtagtgaaa ttgagcagtc
     1681 ttcaccatca ggtttaccag agctacattc aatcgatgca ccgcttctgt cagtatccgg
     1741 tgctggctga gggcctccgg caggcagagc ttgggatccg cgtgaaactt ttagccatcg
     1801 gaaaggcggc ctggaaaatg gcggctgtga gcgttaacgc cttggcagaa aggaacatta
     1861 aggccgaggg gctggtgctc accaagcccg gcctcagcca cggcggtatc cccggcctgg
     1921 ccatcctctc cggtggccat ccgctgccta atgaaaacag cctggcctcc agctcgcgaa
     1981 tcgccgcctg gctgaagaaa ctgagcgcca aagacgacct ggtgatcctg ctttccggag
     2041 gcagctccgc gctctttgaa ctgctgcccg aaggattcga cctggccgac ctggccacgg
     2101 aacacaagct cctgctccac agcggaaaag acatcgccga gatcaaccgc gggcgtacag
     2161 ggctttccct ggtgaagggc ggcagggctc tcgaactcac caaagcccgg aggataaagg
     2221 ttttcgccgt ttccgacgtg gagggaaacg acccccaggt attgggctca gggccgttca
     2281 caccagcgga accgggtgaa aaaacagccg acggctggcg ctataaactt gggaccaaac
     2341 agatcgaata caacatcgtg gcggacaatc agggctttcg cgaacttttt gccaaagact
     2401 tgcgcgaaaa gggcttccgg gtgctggtgg aaaaatcatt tcagggctgt ccactgccta
     2461 agctggccaa ccaaatcaaa gagatcctgc ggcggacaag gagtcccaga tacaggctgc
     2521 gtccgccctt cattaaaatc ctgggcggcg agtcaccgct gaaagtgagc ggcgaaggcc
     2581 tcggtggccg ctgcagccat ctggcgctgc tgctggtgcg ctatcttgca aaagaggagg
     2641 aaactgccct gttctgcttc gccaccgacg gctgcgacaa cgtttccagc agcggcggag
     2701 cctggtccga cagcaacacc ctggcccagc tcaaggccgc gggaatcaac ctcgccgccg
     2761 ccatcaggaa gtgcgacagc tataccgccc tgaaatcggt caaccacctc ctccccgccc
     2821 cgcttctcgc caccaacgtg aacgacatcc acgtcctcag cgtgggctac agcctgcaaa
     2881 gcccctgtcc atccagggac tgggaagcga tggatatctt tgacgaattg gtctgacacc
     2941 cccataccac gatgagaaga ttggcgctgc tcctcacttt gcttctggct ttgggctgga
     3001 cctggctgct ggcgcaggag gaaaccgagc ttcccgcgtc cgtggctccc gccgattcgt
     3061 tgctgaaaac cccggcccca gaggattccc tattttacgc agctgacagc ctggcctaca
     3121 accacgcgct ggaacaaatc cgtctctacg gcaacacaag catcacctac cagactttcg
     3181 agatcagcag cgattcgctt ttggtggacc tcaaaaacaa cctcgccttc agccagagca
     3241 acaccgtgat gcgggacggc gaccagatca tcctgggcga cgacgtggcc tacgacatag
     3301 acagccagac aggttctctt tccggcggta tcagccggat ggagaaaagc tactacacag
     3361 gcgaggaaat ccgcaaaatc agctccgatg cttacgacgt ggacaatggc agcttcacca
     3421 cctgcgagaa cgaggagccg gatttctggt tcaccgccaa aaagctgcgg atttttcggg
     3481 gtgacaaaat cgtgggcaaa cctgtggtcg cctacgtgaa ccacctgccc gtcttctatt
     3541 tccccttcat cgtggtgccg ctccagagcg gacgcaaacc gggtttcctg attccccagc
     3601 cgggctacaa caccatcgac ggcaagtttt tccgcgatat cgcctggtat tatccctaca
     3661 aagactacgc ggacctcatc ctgagcctgg acctttacga gcggacaggc tggcgcgcag
     3721 agctccagct tgactacctg cgccgctatc tactgaacgg cagcctcgac gctgccttcc
     3781 aacgcaggtc gggcggcagc cagaccttca acgactggtc gctgcaagcc actcaccatc
     3841 aggaattggg caaccgtgcc accttcgacg ccaacgtgga tttcgtaagc aacaaacgca
     3901 tctgggaagg aagctccgac atcgacgaaa gcctggccca aacggtcacc tcctctcttt
     3961 cctaccgcca gccgctgctg ggctcgtatc tgaacatcgg agcatcctat accgaagacc
     4021 tgatcaacag ccgcggcacg gtctcgctgc cctcggcctc tttcgccctg cccacccggc
     4081 cgctctatga acttttctat aagccggaac gcagctctga cgcctggtgg agcaacctca
     4141 actacagcta caacctgcgc ctggaccaca gcggcagcct ttactccagc gaccgcctgt
     4201 tcatggatta cgtctggaat aaccgcttcg ccccggggga ttccgtctgg cacaacttcg
     4261 gaatcaagca ccagcttggc ctttcctaca actggaaggc cttcggctgg ctgggtctgc
     4321 agcacggtgc ccgctacaac gaggcctggt ttgaccgcga caaaaacggc aatactctgg
     4381 tgcgcggcaa cgactacaac gcttacacca acgccaactt caacatctac ggcatccgca
     4441 acttcaagaa gggctggctc aaatccctgc gccacatcgt cacgcccaac gccgggataa
     4501 gcttcaatcc tgatttcagc gccaattcac gcttctacag ctttggcggg gtcgggctgc
     4561 tcaattccgc ccgctcggcg aatctcaacc tttcgctgga ccagaaatgg cagatcaagt
     4621 tcggcaaaga cgaccgcaag ataaacgatg tgttcagcct caacagccgc agttcggcca
     4681 atctgctgaa tccttccgcc aagcccttcc aaaccatctc ccacacggcg gctttccgtc
     4741 ccggcagctt cagcctgggc aacctcaggt ttcccggcac caaaacgaag ctggaagggc
     4801 tttcgctggc ctattctgcc cagttcagcc tgagccacag cccctacgga gtgagctggg
     4861 acgactggaa aatcagcaac cagtattttt cccagggcct caccctaaca ggctccgcgc
     4921 cctatcagga ttattttccc aaggccaaaa accgcatttt cgacccctac cagaaagcgg
     4981 acagcctcca gtttttcgcg cagacgctct ccgaagccga atccagcaac acctggagga
     5041 tagccctttc ccacgatttg agcgccaccg ccgacctttt cgaccccatt tcccaaagcc
     5101 ttcgtctgga cagctctttc cgcctcacca aaaactggag cgtggcctat ggcagctatt
     5161 tcaacctgaa aaccggagaa cagctttccc agtcaatccg cgtttcccgc gacctgcact
     5221 gctggaagct tgaactcagc tacacccgcc gcaacgatta ctgggagtac aaagtggcgc
     5281 tgttcaacct ggccctgccg gacgccttga ggttccaaac cagtgatagc aaaagctttt
     5341 aggcgtgcgg ttttcctgga tcgagacggc tgcgtcagcc cggacgagtt cggctatatc
     5401 agcaaccccg ccggctatca gctctacccc tggaccggag aggctttgcg cctgctgcgg
     5461 tccctgggct acctgctcat cctcgtcacc aaccagtcag gcatcgccag aggctatttc
     5521 gacattccag ccctgaaccg cgtgcacgac agactgcaag agcttttggc ggcggagaat
     5581 gtgaaactgg acggcatcta cttttccccc tatttcgccg acggcagcgt tgccccctac
     5641 aacatcgagc acgaggaccg caaaccgggg ctgggaatgt tccgcaaagc ccggcgcgca
     5701 cacaggataa atcccggcca atcctggatg ataggagaca ggcaaacgga catcactttc
     5761 ggcaagaacg cgggcctaag ctccatcctg cttctcaccg gcaacggcgc ccgggaaatg
     5821 ctttccggcc tgcaagactg gccgcggcag ccggattaca tcgccgaaaa cctgctcacg
     5881 gcggccaaac tgatcgaact tctccgctga tgaagacccg cggctctttc agccagagaa
     5941 agttccaaag cctttcccct tccgcccagg tcaaggctct ggacaagctc gcgtctgtcc
     6001 tggaaaaatc acttgctggc agagaaccgg cttccgaatt gacctcacac gtgctggaat
     6061 gcgtctcctg gatggctgaa cccgtgccgg agaacgttct caggctttcc caggacctgg
     6121 catccgccgc cgcgaccagg gaggcccaac tggcgataac ccgcttcaaa agttcgctgg
     6181 gccaatcttt caaggataac cagatacccc tcaaacgagg ggacggaccc cgcttcgccg
     6241 acctgaacac cctcgagcgc gctggcaagg tcatcctcat tctggacaac ctccgttcag
     6301 ccttcaacgt gggttcgatc ttccgcagcg ctgagtgtct gggcctttgc aaaatctggc
     6361 tttgcggagt aaccgccacc ccgaatgacc acgctttgcg gaaaaccgcc cggggaaccg
     6421 cctcccgcat cgactggagc cactttgagg aaacggcgga ggcggtgaaa caggccagag
     6481 ccgaaggata ccgggtttac gccctggaaa tctcagccca ggcggaatcc gtcttcgcgg
     6541 aggattttca gctccccctc gccctggtgc tcggaaacga aagcctgggc atttcaaacg
     6601 aaattctgtc cctttgcgac cggacggttt gcctgccggt ccagggctgg aaaaactcgc
     6661 tcaacgtggc cgttgcctgc gccgtatgcg cctaccagat cgtgttcggc tgcccatcca
     6721 gccattgaaa gggatttgac taatccacaa cacttctcca gcttcctgaa ctaaccggcc
     6781 tgttccaaac aggtttcccg gtagtcagac ccttagcgct ctcttttatc cccgtcgtat
     6841 gcctcccgta gaatactcgc atcgagtgcg gccattgtgc gggaggcgag acagggacgc
     6901 aggaaagggc cttaacggcc aagcagcaat gcttccttcc tctgattatc agccttgctc
     6961 ccctccaccg tgaatttcag ccaaagaaaa accccgtcgt ttgacggggt tctgataacc
     7021 tattggagtt tatggctcag ttgaatctaa gcaccacaaa ttgctctgtc ttgttgcggt
     7081 cctgcacgta gaggatgatc gtgttgcgtt cgtccttccg cattttatcg atggcggcgt
     7141 tcatggcggc gctgtattct tccgggctgt tcacctcggt atttcccacc ttgaggacca
     7201 cgaagccaac ctggatccct gccttggaag ccggggaatt ggattcgatg gcgctcacga
     7261 tcacgccttt gctcgccttt atgttgttgc gttgggcata ggcgctgtcg gtggcttcca
     7321 cgctgatgcc tgctccgctg tccggggccg ctgcttcttc agtctcggcc acggtgtcct
     7381 cggggaagtt gtcgagctgg atgtagaggg ttttgacctt atcctcgcgg atgattttca
     7441 ccggaacctt ggttcccact tggaagt
//
LOCUS       DAOOWR010000037         7449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_9936_length_7449_cov_3.806465, whole genome shotgun sequence.
ACCESSION   DAOOWR010000037 DAOOWR010000000
VERSION     DAOOWR010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_7449_cov_3.806465"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PLB85_01780"
     CDS             <1..1300
                     /locus_tag="PLB85_01780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOU72085.1"
                     /translation="HFRQRFINEARILSQLQHPHIVGLFTFFLEQESYFMVMEYAEGQ
                     TLRDLIATVGPIPEQRTRRILRQILSALDYAQTKGVVHRDIKPSNIMIGENDDLKILD
                     FGVARILANPQFTLAGSRLGTIYYMSPEQVRSPRDVDSCSDIYSTGVVLYEMLTGRLP
                     FSTETESDFLIEKQIVESPMPHPRDYYPYMSDHIVDLMNAMTAKEPSLRPTAAEAIRA
                     LDTSSLDFIKTAPPLSEKPAPVEPVAPTPIPPAPKKGMPPGLRAFLVIFIIAIILVTL
                     GFMLKAWLDINPNLFGPRSEEAVAEETVEGPASEKETLSQEEIEANIAARKRREAENA
                     VRSIRPRFSTIARKAREYKAQDEYRDWPKSLDAFIDPAEADTDQFNFDWSDDGIIIAI
                     SNRAFGKAGIEIYYTLDSDHFEIYDPDPENPPNIDPAWLN"
     gene            1927..3207
                     /locus_tag="PLB85_01785"
     CDS             1927..3207
                     /locus_tag="PLB85_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOU72086.1"
                     /translation="MPLSERPYAEFLPEEVWKWKLMEERFDRVLKLHNYQEIRLPILQ
                     NRDLVHKGITALMPGREAARLAAKTLDICGHDGGPGNLSLRPECTISVLSYAARTYRR
                     GDIHRYYYHGPVFRQGRDQEPRESFQLGVELLGSSSLISENEVISLGIRLLRDLGFRD
                     ASLRLNSFGCEICREKFCKAVRAEMEKHSAEYCLACLDELGANPFTETHCEDERCRHR
                     VPGELRILDHLCKRCKAKFSSIQKVQANLGHIYKVDPGLFKNFAYYNETVFDLVIGEG
                     EKEMTVGGGGRYDNLSEKITKLRIPAVGFYLDLDLVFAAMDARRLFFDWNRDFSVYIC
                     AQSQDMEMMLLQIAQELHSEDIITVLSPDILESGEEQTRARARNCDLMIAIREENIRE
                     GKLLLFNLARDTQAYIPLHRLAESVQLARKTLRK"
     gene            3217..3588
                     /locus_tag="PLB85_01790"
     CDS             3217..3588
                     /locus_tag="PLB85_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU72087.1"
                     /translation="MQAISTIKAPAALGPYSQAVIMNDALFVSGQLGIEAETGNFGEG
                     FEAQANLVFANLKAILEAAGMSFRDVVKVSVFLKDMNDFAALNEIYARNFSAPFPARE
                     AIQVARLPKDGLVEISVIAIK"
     gene            3585..4115
                     /locus_tag="PLB85_01795"
     CDS             3585..4115
                     /locus_tag="PLB85_01795"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU72088.1"
                     /translation="MSIITKTGDGGQTSLYGGERVWKDDLRVRAYGALDELDAHLGDA
                     RHQISDDRALSIILDIQNALCRLMGELATPGGNYQNPVKEGDVEAVGELVRELEADTP
                     ITGFVIPGSLPASAKLDICRAVARRAEREVVALARKENVSPPLLRFVNRISDLLFILA
                     RALEAKEGAIRYKTTT"
     gene            4120..4875
                     /gene="gpmA"
                     /locus_tag="PLB85_01800"
     CDS             4120..4875
                     /gene="gpmA"
                     /locus_tag="PLB85_01800"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOU72089.1"
                     /translation="MKMYKIVLVRHGESIWNKENLFTGWTDVDLSEKGVREARIAGQR
                     LAAAGFEFDEAWTSVLKRAIRTLWLVLDEMDLMYLPIHKDWRLNERHYGALQGLNKAE
                     TAAKYGEEKVMTWRRSFDVPPPALELTDPRHPGFDPRYAGLEKDRLPLCESLKDTCER
                     AMPFWDEVIVPRLKAGRRMIVSAHGNSLRAIVKNLDGISDADIVGLNIPTGIPLVYEF
                     KSDLSVAKRYYLADADELAQAQKVVADQGKAKE"
     gene            4939..5109
                     /locus_tag="PLB85_01805"
     CDS             4939..5109
                     /locus_tag="PLB85_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU72090.1"
                     /translation="MAVKIDKDTCIGCGACVDVCPVQALSLEDGKAKCDEGTCIDCGA
                     CIGTCPVQAISE"
     gene            5199..>7449
                     /locus_tag="PLB85_01810"
     CDS             5199..>7449
                     /locus_tag="PLB85_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU72091.1"
                     /translation="MRRAIAILFIVLPFCWLAATDVSGNQSGTWTLANSPYNIIGDVT
                     VPAGDSLSIEPGVEVFVIGNYRLKAEGKLTAVGTEADTIRFRSGQADPGALWKGIRLE
                     NESLQSQIRRCYIEKAEYGVNAIDSPVLISHCRFNLNDKGMQLYGIGSADPATMLVQY
                     NIVENSIGNGILISQNSNALIEYNEVRFNGTGSQYRGAIQLSNQSAGGSNSPMIRYNH
                     IHHNHKQGITAWDIQAVNAIQPHILENVIEYNLTGIYLLNASGYVADNIIRHNFIPGD
                     ANSGAGVMVAGATSEPYFERNIIHGNFTGFYLGNNAKPCLGNLDIYHAWAQGENQIYD
                     NIDGSNIPHSVYTYSYTTPGLTIWAENNSWGTNDPAEIAIGINDQNDDPSLPLVDFEP
                     ILPIPVPTTVTGSVSYTGAFSLTNHRVQIVGADSGEIYYEEPVSPDQPFTLSQIFDGQ
                     FHVVVMADVEGVERVFYGTPGGLLTPETFSPDGLEPVEAGVIQIQETQPPRFQDVGSP
                     EQIGAHLCYPVYHRFFIYRWDHINWLYESGDYVFLKRHERFNEAGNIIFNFADEVVWD
                     KISGIEHNNTWLRTEILNDSGAQRVSMFRAKIVTTASDGSNTHMLILQSDTVNQQLIS
                     VRVMDEGLSRVYHYNAGYLANCETVVGNIVFQYLHEGNWWFYQPLLPVYQPTGLCVDQ
                     VEHFVESPWALTLYWQAPADDGIYNWTHYRVYNYDTLLAEVPYLLNSWHSNNFNYQDE
                     HCLTVCAFDG"
BASE COUNT         1726 a         2325 c         1983 g         1415 t
ORIGIN      
        1 gcatttccgc cagcgcttca tcaacgaagc ccgcatcctg tcacagcttc agcatcccca
       61 catcgttggc ctcttcactt tcttcctgga gcaggaaagc tatttcatgg tcatggaata
      121 cgccgaaggc cagaccctgc gcgacctcat cgccaccgtc ggccccattc cggaacagcg
      181 tacccggcgc atcctccgcc agatcctttc cgccctggat tatgcccaaa ccaagggcgt
      241 tgtccaccgc gacatcaagc cctcgaatat catgatcggc gaaaatgacg acctcaagat
      301 actggatttc ggcgtggccc gcatcctggc caatccccag ttcaccctcg ccggctccag
      361 gctgggcacc atctactaca tgagcccgga gcaggtgcgc tcgccgcgtg atgtggacag
      421 ctgttcggac atatattcca ccggtgtggt cctctatgaa atgctcactg ggcggttgcc
      481 cttcagcacg gaaaccgaga gcgatttcct catcgaaaag cagatcgtgg aaagccccat
      541 gccgcatccg cgtgactatt acccctatat gtccgaccac atagtggacc tgatgaacgc
      601 catgacggcc aaggaacctt cgctgcgccc caccgccgcc gaagcgatca gggctttgga
      661 caccagctcg ctggacttca tcaaaaccgc cccacccctc agcgaaaagc ccgctcccgt
      721 cgagcctgtg gcccccacac ccattcctcc ggcccccaaa aagggaatgc cgccgggact
      781 gcgcgccttc ctcgtcatct tcattatcgc catcatcctg gtcacgctcg gtttcatgct
      841 caaggcgtgg ctggatatca atcccaacct cttcggcccc cggtccgaag aagccgtggc
      901 ggaggaaacg gtggaaggac cggcaagcga aaaagaaacg ctcagccaag aggagatcga
      961 ggccaatatc gccgcgcgga agcgccggga agccgaaaac gccgtccgct cgatccggcc
     1021 ccgcttcagc accatcgcca gaaaagcccg ggaatacaag gcccaggatg aatacaggga
     1081 ctggccaaag agcctggacg ccttcattga ccccgcggag gccgacaccg accaatttaa
     1141 cttcgattgg tctgacgacg gcatcatcat cgccatcagc aaccgcgctt tcggcaaagc
     1201 cgggatagag atctactata ccctcgattc cgaccatttc gagatttacg accccgaccc
     1261 ggaaaacccg cccaacatcg accccgcctg gctgaattga ccgaccggca gacctggctc
     1321 caaacatcgc agccatgcaa gctggccgtt aaggcccttt cctgattccc agacgcgcct
     1381 cccgcactat ccccgcattc gatgcgagcg tcattcgagt ggcgtgggag ggcgatagtc
     1441 gcaggcgctc agggcctgac tgacaggaca ttgcggtggg caagcaatta tggccctcct
     1501 ttaaaacatc gctttctttc aaatagagta ggtagtccgg ttgatcgagc ccgttgggcg
     1561 acataacgat agcgatggtg cgtaaccccc tcgtacaagt gcgataacaa aatggaaaac
     1621 tccttgtggg cgacagaaat caatccccgc ggttctttca cccgcaagtg gctcagaagg
     1681 tagttgggat ccattgtcga ggggttccgt tcgctgcgct cactgcacac cctcgctatg
     1741 gtctgccgcc tctgcgaggc tttacccatc cggttcgaaa gagagcctga aacaagtggt
     1801 tgccgacact caacctgaaa gcggtcggga tggcctcatc caccctgatt gaacatgaac
     1861 catccttttc attgacagaa aaagcggtaa attcagcatg gattaaccaa atcaagagga
     1921 gaaaaaatgc ccctgtccga gcgcccttac gcggagttcc tgcccgaaga agtgtggaag
     1981 tggaagctga tggaagagcg tttcgaccgt gtgttgaagc tgcacaacta tcaggagatc
     2041 cgcctgccca tcctgcagaa ccgcgacttg gtgcataagg ggatcacggc gctgatgccg
     2101 ggccgggaag cggcgcggct cgcggcgaaa accctggaca tctgcggcca tgacggcggt
     2161 ccgggcaacc tgagcttgcg tcccgaatgc accatcagcg tgctcagtta cgctgcgagg
     2221 acctaccgcc ggggcgacat ccaccgctat tactaccacg gcccggtttt ccgccagggg
     2281 cgtgaccagg agccgcggga atccttccag ctcggggtgg agcttttggg cagttccagc
     2341 ctgatttcgg aaaacgaggt cataagcctg ggcataaggc tgctgcgcga tttgggcttt
     2401 cgggacgcca gcctgcgttt gaacagcttt ggctgcgaaa tctgccggga gaaattctgc
     2461 aaagccgtgc gtgcagagat ggaaaagcac agcgctgaat actgcctcgc ctgcctggat
     2521 gaactggggg ccaatccctt caccgaaacc cattgcgagg atgaacgctg ccgccacagg
     2581 gtgccgggtg aactcaggat cctcgaccac ctctgcaagc gctgcaaggc caaattctcc
     2641 agcatccaga aggtgcaggc caatctgggc catatctaca aagtggaccc gggcctgttc
     2701 aagaatttcg cctattacaa cgaaaccgtg tttgacctgg tgatcggcga aggtgagaaa
     2761 gaaatgaccg tgggcggcgg cggccgctac gacaacctct cggaaaagat aaccaaactg
     2821 cgcatccccg ctgtgggctt ctatctggac ctcgacctcg tgtttgccgc catggatgct
     2881 cgccggctct ttttcgactg gaacagggat ttctcggttt atatctgcgc gcagtcgcag
     2941 gatatggaga tgatgctgct gcaaatcgcc caggaactgc attcggagga tatcatcacc
     3001 gtcctcagcc cggatattct cgaaagcggg gaggaacaaa cccgtgcccg ggccaggaac
     3061 tgcgacctga tgatagccat ccgcgaggaa aacatacgcg aaggcaagct gctgctcttc
     3121 aacctggctc gcgacaccca ggcctacata cccctgcacc gcctggccga atccgtccaa
     3181 ctggcgcgta aaacactaag aaaataagga gataatatgc aagctatcag cacaatcaaa
     3241 gctcctgccg ccttggggcc ctattcccag gccgtgataa tgaacgacgc cctcttcgtt
     3301 tccggacagc ttgggatcga agccgaaacg ggaaatttcg gcgaaggctt cgaagcccag
     3361 gcgaacctcg tgttcgccaa cctcaaagcc attctggaag cagcgggaat gagcttccgc
     3421 gacgtggtga aagtctccgt gttcctgaag gacatgaacg attttgccgc cctcaacgag
     3481 atttacgccc gcaacttcag cgcccccttt cccgcccggg aagccatcca ggtggcccgg
     3541 ctgcccaaag acggattggt ggaaatctcc gtcatcgcga taaaatgagc atcatcacga
     3601 aaaccggcga cggaggccag acctcgctct acggcggaga acgggtttgg aaagacgatc
     3661 tgcgcgtgcg cgcttacggc gctttggacg aactggacgc ccatctgggg gacgcgcgtc
     3721 accagatttc tgatgatcgg gcgctgtcaa taatcctgga catccaaaac gccctttgcc
     3781 gcttgatggg tgaactggcc acccccggcg ggaactacca aaatcccgtt aaggaagggg
     3841 atgttgaggc ggtcggagag cttgtccgag aactggaggc tgacacgccc ataacaggtt
     3901 ttgtgattcc cggctcgttg ccggcttcgg ccaaactgga catctgccgc gctgtggccc
     3961 gccgcgccga gcgcgaagtg gtcgccctgg cccggaagga gaatgtttcg ccgccgctgc
     4021 tccggttcgt gaacaggatt tcggacctgc tgttcatcct ggcccgcgcc ctggaggcga
     4081 aagagggggc catccgctat aaaacaacaa cctaaggaga taaaaatgta taagatagtg
     4141 cttgtccgcc acggcgaaag tatttggaac aaggaaaact tattcaccgg ctggaccgac
     4201 gtggaccttt ccgaaaaggg cgtccgggaa gcaaggatcg ccggacagcg cctggccgcg
     4261 gcgggctttg aattcgacga agcctggacc tcagtgctca aacgcgccat ccgcaccctc
     4321 tggctggtgc tcgacgagat ggacctcatg tatctgccca tccacaaaga ctggcgcctc
     4381 aacgaacgcc actacggagc cttgcagggg ctcaacaaag ccgagacagc cgccaaatac
     4441 ggggaagaga aggtgatgac ctggcgccgc agcttcgacg tccccccgcc cgctctggaa
     4501 cttaccgacc cgcgccatcc gggcttcgac ccccgctacg ccggtctcga gaaagaccgg
     4561 cttcccctct gcgaatcgct gaaagacacc tgcgaacgcg ccatgccctt ctgggatgag
     4621 gtgatcgtcc ccaggctgaa ggccgggcga aggatgattg tttccgccca cggcaacagc
     4681 ctccgcgcca tcgttaaaaa cctcgacggg atcagcgacg cggatatcgt cggcttgaac
     4741 atccccaccg gcataccgct ggtttatgaa ttcaaatccg acctgagcgt ggcaaaacgc
     4801 tattacctgg ccgacgcgga cgagctggcc caagcccaaa aagtggtggc tgaccagggc
     4861 aaagctaaag aatagatttt ccttgacgaa tccggctttg gcaggaatct tggacaaaac
     4921 cttatacagg agatagaaat ggcagtcaag atcgataaag acacttgcat cggatgcggg
     4981 gcctgcgtgg atgtttgccc cgtgcaggct ctttccctgg aagacggcaa ggccaaatgc
     5041 gacgaaggca cctgcattga ttgcggcgcc tgcatcggca cctgtcccgt gcaagccata
     5101 tcagaataag cttatttcgc aagcttgaac gcggaagagg gcgatgcctt cttccgtttt
     5161 tgtttctcag agcaccatta cagaccctgg aggagattat gcgacgcgca attgctatct
     5221 tatttatagt cttgccgttc tgttggctgg ctgccacgga tgtgagcgga aaccagtccg
     5281 gaacctggac tttggccaac agcccctaca acatcatcgg agacgtgacc gtcccggcgg
     5341 gcgacagcct cagcatcgag cccggcgtgg aggtttttgt gataggcaac taccggctca
     5401 aggcggaagg aaagctcacg gcagttggaa ccgaggctga caccatccgc tttcgcagcg
     5461 gccaggcgga ccccggcgcc ctctggaagg gcatccggct ggaaaacgag agcctccaga
     5521 gccaaatccg gcgctgctac atcgaaaagg ccgaatacgg ggtcaatgcc atcgactcgc
     5581 ctgtgctgat cagccactgc cgcttcaacc tgaacgacaa gggaatgcag ctttacggca
     5641 ttggctccgc cgacccagcc accatgctgg tccagtacaa cattgtggaa aacagcatcg
     5701 gcaatggcat cctcatttcc cagaacagca acgccctcat cgaatacaac gaggtccgct
     5761 tcaacggcac cggatcgcaa taccgcggcg ccatccagct ttccaaccaa tcagccggag
     5821 gctccaacag ccccatgatc cgctataacc acatccacca caaccacaaa cagggcatca
     5881 ccgcctggga tattcaagcc gtgaacgcca tccagcccca catcctggaa aacgtcatcg
     5941 aatacaacct caccggcatc tatctgctca acgcctccgg ctacgtggcg gacaacatca
     6001 tccgccacaa cttcattccc ggcgacgcca attccggggc cggggtcatg gtggccggag
     6061 ccaccagtga gccttacttt gagcgaaata tcatccacgg caatttcacc ggcttctacc
     6121 tcggcaacaa cgccaaaccc tgcctgggaa acctggacat ctaccacgcc tgggcccagg
     6181 gcgaaaacca aatttacgat aatatcgacg gttccaacat cccgcacagc gtttacacct
     6241 attcctacac cacccccggc ctcaccatct gggcagaaaa caacagctgg ggaaccaacg
     6301 accccgccga aatagccatc ggcatcaacg accagaacga cgaccccagc ctgcccctgg
     6361 tggatttcga gcctatcctg cccatacctg ttcccaccac cgtgaccggc tccgtcagct
     6421 acaccggggc cttcagcctc accaaccacc gcgttcagat cgtgggagct gattcaggcg
     6481 aaatctatta tgaagaaccg gtgagcccgg accagccttt cacgctttcc cagatatttg
     6541 acgggcagtt ccacgtggtg gtcatggccg atgtggaagg ggtggagcgt gtcttctacg
     6601 gcactccggg cgggctgctg actccggaga cattctctcc ggacggtttg gagcccgttg
     6661 aggctggcgt catccaaatc caggagacgc aaccgccacg ttttcaggat gtgggttcgc
     6721 ccgaacagat cggcgcccat ctctgctatc cggtttatca ccgctttttc atttaccgct
     6781 gggaccacat caactggctt tacgaatcgg gcgattacgt cttcctcaaa cgccacgaac
     6841 gcttcaacga agccggcaac atcatcttca acttcgccga cgaagtggtc tgggacaaga
     6901 tcagcggcat cgaacacaac aatacttggc tgcgcaccga aatcttgaat gacagcggag
     6961 cccagcgcgt cagcatgttc cgtgccaaaa tcgtgaccac agcttccgac ggctcgaaca
     7021 cccatatgct tatcctccag tcagacactg ttaaccagca actcatctcc gtccgcgtga
     7081 tggacgaggg attgagccgc gtttaccact acaacgcggg ttacctggca aattgcgaaa
     7141 ccgtggtcgg caacatcgtg ttccaatatc tgcacgaggg aaactggtgg ttctaccagc
     7201 ccttgttgcc ggtatatcag cccaccggcc tctgcgttga ccaggtggaa catttcgtcg
     7261 aaagcccctg ggccctcacc ctctactggc aggctccggc ggatgacggt atctacaact
     7321 ggacccatta ccgcgtttac aattacgata ccctcctggc ggaagtgccc tacctgctta
     7381 atagctggca ctcgaacaac ttcaattacc aggatgaaca ctgcctcact gtctgcgctt
     7441 tcgacggcg
//
LOCUS       DAOOWR010000038         7326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10175_length_7326_cov_3.472287, whole genome shotgun sequence.
ACCESSION   DAOOWR010000038 DAOOWR010000000
VERSION     DAOOWR010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10175_length_7326_cov_3.472287"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PLB85_01815"
     CDS             complement(<1..962)
                     /locus_tag="PLB85_01815"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72092.1"
                     /translation="MKKLLILLIAAFVFGGLFAAPLTEGFESPACPAAGWTIRYANQS
                     YPSGNTMTHSTNQYYAGARSFRFSSYSSGAPYDQYLITPELNVTSGDQTVSFWYRKHD
                     YGSETFRVGWSSTGTEVADFFWSADITNASTNWQQYTKTDLPVGTKYVAVHYKSNYAY
                     YLYIDEFVGPNISVAPEPSNHVTGFAAGNLALTSIQLNWTGSTGTQLPHGYLIQAITG
                     AGSYAAVADGTPVASDIDWSDGNAAINVSHVVGANTYTFTGLTANTAYEFKIWPYTNN
                     GADIDFKVDTPIPTVNASTPDPVKPLPYTQDFNSGTSLSAIEWAG"
     gene            1916..2353
                     /gene="rplM"
                     /locus_tag="PLB85_01820"
     CDS             1916..2353
                     /gene="rplM"
                     /locus_tag="PLB85_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU72093.1"
                     /translation="MKTLTPKPDDIQRAWYVVDATDLPLGRLSTRVASILCGKNKPYY
                     VRNIDTGDYVIVINADKVRVTGLKSLQKIYKTYSGYPSGLKEIPFQRMLEKHPTQIIE
                     HAVRGMMPKTVLGDAMFKKLKVYAGTEHPHAAQKPVELNLKEK"
     gene            2350..2742
                     /gene="rpsI"
                     /locus_tag="PLB85_01825"
     CDS             2350..2742
                     /gene="rpsI"
                     /locus_tag="PLB85_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU72094.1"
                     /translation="MSMQTYDAVGRRKNAVARVRIAPGTGKRIINKVQMKKYLQRETL
                     EMVVEQPLQELGVSDSFDVYVNVTGGGLSGQAGAIRHGISRALVEYDEKYRPALKARG
                     FLTRDPRMVERKKSGRPKARKRFQFSKR"
     gene            2909..3730
                     /gene="rpsB"
                     /locus_tag="PLB85_01830"
     CDS             2909..3730
                     /gene="rpsB"
                     /locus_tag="PLB85_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU72095.1"
                     /translation="MSVVTMKQLLEAGVHFGHQTFKWNPKMKKYIFIKRNGIHIIDLK
                     QTVDAINEAYQFLKEVANRQEYILFVGTKKQAQAAIKDAAEKSGVFYVNQRWYGGMLT
                     NMATIRKSIEKMRYYEDIVADGTINNYTKLEQQKMKRMHDKIEFSLGGIREMDALPGA
                     IFIVDTGHEEIAVHEARILGVPIIAMVDTNCDPDLIDYVIPSNDDATRAIQLISDIMA
                     NAVLEGKNIAAEGADTQEEPAEFTPEMAETTAEPVQSEKPAEQPEEMAAAAEEEK"
     gene            3798..4397
                     /gene="tsf"
                     /locus_tag="PLB85_01835"
     CDS             3798..4397
                     /gene="tsf"
                     /locus_tag="PLB85_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOU72096.1"
                     /translation="MIEVTAKMVKELRDRTGVGMMECRKALLETNGDVDAAIKYLRER
                     GISKAEGKALRETKEGIIFSYIHFNNRLGVMLELNCESDFVARTDEFKKLAEEIALQI
                     AATNPLAVSADQIDPAIIEREREIAHNKAVNEGKKPEIIDKIVEGSIKKYRSEHSLLE
                     QELVSESGRTVKDMLTEAISRTGENIQIARFTRFALGGE"
     gene            4428..5150
                     /gene="pyrH"
                     /locus_tag="PLB85_01840"
     CDS             4428..5150
                     /gene="pyrH"
                     /locus_tag="PLB85_01840"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU72097.1"
                     /translation="MTYKPEKIHRLLLKISGEVLAGPKGFGYDDAVIDVLTDEIIEAR
                     RLGYSLAIVLGGGNIFRGGTWKNQSLNRVTLDHIGMLATVQNSLYLAEILNRKNYSAQ
                     VFTSFSIDSVAMHYTPRRAFQAMEAGSICLLSGGTGNPFFTTDTAAVLRACELKLDMV
                     FKGTKVDGLYSADPKVDPTAEFISDATYDQCLEQKLGVMDMTAFSLAQANELPIKIFN
                     IGKQGGLCQAISSPDHGTFIHP"
     gene            5176..5730
                     /gene="frr"
                     /locus_tag="PLB85_01845"
     CDS             5176..5730
                     /gene="frr"
                     /locus_tag="PLB85_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU72098.1"
                     /translation="MEQLISQSQDKMNKSFDALLHQFSRVRTGRASASILDDVRINYY
                     GSPTPVKQLCNISIPEPRTIVVQPWDKTTLADIEKAILAANLGITPENDGNVIRLPFQ
                     PLTEEKRRDIVKNLKKLAEDARVAIRNIRREANDQAKKMEKDSEISEDEQKKLLKDIQ
                     DLTDEWVKKIDEVEKGKEKEIMEV"
     gene            6659..>7326
                     /locus_tag="PLB85_01850"
     CDS             6659..>7326
                     /locus_tag="PLB85_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU72099.1"
                     /translation="MIKMRTLLPMLLVLSLGFGCACNKKKTDITPVSDEVNISLGEQN
                     NAFAFNLYALADEPGKNLFYSPHSITSALSMVYGGAQGNTAKQMAKAMSFNLPAEEQH
                     KSYLSLQQSLNALGEKGKAELNVANALFGSTTNEKFIRKDYLKLLRECYLSDLYSLNF
                     SDFSGTAKFINTWVEGKTKDRIKNLVTEDHIRDSNEGMVLVNAIYFKGSWLNEFEPKL
                     TMKD"
BASE COUNT         1924 a         1927 c         1957 g         1518 t
ORIGIN      
        1 ccggcccact caatagcaga cagggatgtg ccgctgttga aatcctgtgt gtagggcagc
       61 ggcttgacgg ggtcgggtgt agaggcattc accgtcggga tgggagtgtc aaccttgaag
      121 tcgatgtctg ccccgttgtt ggtgtagggc cagattttga attcatatgc agtgttggcg
      181 gtcaatcccg taaaggtgta ggtgttggca cccaccacat gggatacatt gatcgcggcg
      241 ttgccgtctg accagtcgat gtcactggca accggagttc catccgcgac cgcggcatag
      301 gagccagcac ctgtgatcgc ctggatcagg tatccatggg gtaattgtgt accggtggaa
      361 cctgtccagt tcaactggat ggatgttaaa gccaggttgc ctgcggcaaa acccgtcacg
      421 tggttgcttg gctcgggggc aacactaata ttaggcccca caaattcatc aatgtagagg
      481 tagtatgcgt agtttgattt gtagtgcacg gcaacatatt ttgtgccgac aggcaggtca
      541 gtcttggtgt attgctgcca attggtcgat gcgttcgtta tatctgctga ccagaagaag
      601 tcagcaactt ctgtaccggt cgaagaccat ccaactctga aggtttcaga accgtagtca
      661 tgttttctgt accagaagct gactgtttgg tcacctgatg tgacattgag ctctggagtt
      721 atcaggtact ggtcgtaagg ggctccggag ctatacgaag agaaacggaa agaccgggcg
      781 cctgcataat actgattcgt gctatgcgtc atggtattgc cggaggggta agactggttg
      841 gcatatctta tggtccaacc ggcagcaggg catgcgggac tttcaaatcc ttcggttaac
      901 ggagctgcaa acaaaccgcc gaacacaaag gcagcgatta gcagaattag taatttcttc
      961 atatcatctc tccttaaaga tagatagagt tctgaagtta gtgtgaagaa ttctttggtt
     1021 agctagggac cggaatcagc ccgggcagtg aagctcccgg ccggttcgcg gcccctgatt
     1081 tggttttgaa gttgggaatg taagctgttt tccctctttc cggcccgggg aagcgtgcct
     1141 ggtgtaatgt gcgaagttac ctcttgactt gcttccggag gtggagtgat gggaaaaaca
     1201 cgttgggaaa tgagattgaa cgaaattctt gtatttgacg ggggatttgg atgcttctcg
     1261 gctgtttcga acccgcagac cgggcgaaaa aaacggcgtt gggtctgaaa aatcaattct
     1321 catccaatct ccattttggc cggatcctga aaacgcggga tgcctgctgc tgccaattct
     1381 atatcgactg ctgtgaaagt ggcgatccaa acgatttcaa agttatacca gcataagctt
     1441 atttatcgat ttccattttc agagatactg ctaatggtgt caagtgatat tttggacaat
     1501 ttgtttaagg tgcgcgaaaa aagaagaacg caaatatttt tgtgccttgg tgtttcctcc
     1561 ctttggcgaa tttagtggtg tcgcttttgg attttccgga taaggtaaca gcgcttgtgg
     1621 aaataagttc tgcctaacgc gataaacatc gcccgaatgg ctgcaatatt tgcgtaagcc
     1681 gcagtgcatt attcacgaac ctgtcagtcc cgcttatctt ctgaatgcct attgcctttg
     1741 ccagataggg tgttaagcct gctgcttgtt cgcctgtgga aaaaaatcca taatgcgcaa
     1801 aaacataacc agagggaggg gaaatggatg tcgctggagc ggcaatataa tccttgacac
     1861 catccccgct tgaaaaattg tggctttctg tataaatatt tgtaaaggag aatcgatgaa
     1921 aaccctcact ccgaagcccg acgacatcca gcgcgcctgg tatgttgtcg atgccacgga
     1981 tctgcctttg ggacgtcttt ccacacgggt ggcctccatc ctctgcggca agaacaagcc
     2041 ctattacgtg cgcaatatcg acaccggcga ttacgtgatc gtgatcaacg ccgacaaggt
     2101 gcgcgtgacc ggtttgaaaa gcctgcagaa gatctacaag acctacagcg gctaccccag
     2161 cggactcaag gagatcccgt ttcaacgaat gcttgaaaag catcccaccc agatcatcga
     2221 gcacgccgtg cgcggcatga tgcccaaaac cgttctggga gatgctatgt tcaagaaact
     2281 gaaagtctat gccggaaccg agcatcccca cgcggctcag aaaccggtgg aactcaacct
     2341 caaggagaaa tgagtatgca aacttatgat gcagttggca gaaggaaaaa cgccgtggcc
     2401 cgtgtccgga tcgcacccgg aacaggcaaa cgcatcatca acaaggtgca gatgaagaaa
     2461 tacctgcagc gcgaaacgct ggaaatggtt gtggaacagc cccttcagga gcttggcgtg
     2521 agcgacagtt tcgacgttta cgtcaacgtc accggcggtg gcctcagcgg ccaggccggc
     2581 gccattcgcc acggtatttc ccgcgccctc gtcgagtatg atgaaaaata ccgtcctgcc
     2641 ctcaaagccc gcggctttct aactcgcgac ccccggatgg tggagcgcaa gaagagcgga
     2701 aggccgaaag ccagaaagag attccagttc tccaaacgtt aaaccgggtt ttggggaggc
     2761 cagccctccc ccgccgggac agatgaactg ccagatccat ccacccaaaa aaccgcgccg
     2821 ggagttgcaa ggcggtgggg agtccgctcc tctgaattgc ttagatccct gcccggaaaa
     2881 gcaaccgtaa gatatgcaag gagtatcaat gtccgtagtt acaatgaaac aactactgga
     2941 agcgggagtc cacttcggcc atcagacctt caagtggaac ccgaaaatga agaagtacat
     3001 cttcatcaag cgcaacggca tccacatcat cgacctcaaa cagaccgttg acgccatcaa
     3061 tgaggcctac caatttttga aggaagtcgc caaccgccag gagtacatcc tcttcgtggg
     3121 caccaagaaa caagcccagg cggccatcaa ggacgccgcc gagaaaagcg gtgtcttcta
     3181 tgttaaccag cgctggtacg gcggcatgct cacaaacatg gccaccatcc gcaaaagcat
     3241 cgaaaagatg agatattacg aagatatcgt ggccgacggc accatcaaca actataccaa
     3301 gctcgagcag caaaagatga aacgcatgca cgacaagatc gaattctcgc tgggcgggat
     3361 ccgtgaaatg gatgcccttc ccggggccat cttcatcgtt gacaccggcc atgaagagat
     3421 cgccgtgcat gaagcccgca tcctgggtgt gccgatcatc gccatggtgg acaccaactg
     3481 tgaccccgac ctgatcgatt atgtgattcc ctcaaacgac gacgccacca gagccatcca
     3541 gctgatttct gacataatgg ccaacgccgt gcttgaagga aaaaacatcg ccgctgaagg
     3601 cgccgacacc caagaagaac cggctgaatt tacccctgag atggcggaaa ccaccgccga
     3661 gccagttcag tctgaaaaac ccgcggagca gcctgaggaa atggcagcag ccgcggaaga
     3721 agagaaataa accggatttt acgagcgggg agggttcccg ccctccctgc ccccaacaag
     3781 catcaaggag ttttcaaatg atagaagtca cagccaaaat ggtaaaagaa ctgcgcgaca
     3841 gaaccggcgt gggcatgatg gagtgccgca aagccctgct tgagaccaac ggtgatgtgg
     3901 atgccgccat caaatacctg cgcgagcgcg gcatctccaa agccgagggc aaggccctgc
     3961 gcgaaaccaa ggaagggatc atcttttcct atatccactt caacaacagg ctgggcgtga
     4021 tgctcgagct gaactgcgaa agcgatttcg tggcccgcac cgatgaattc aagaagctgg
     4081 cagaagagat cgccctgcag atcgcggcca ccaatcccct ggccgtatcc gccgatcaga
     4141 tcgaccccgc cataatcgaa agagagcgcg aaatcgccca caacaaagcc gtcaatgaag
     4201 gcaagaagcc cgaaatcata gacaagatcg tggaaggcag catcaaaaag tatcgctccg
     4261 agcattccct gctggaacag gaactggtga gtgaatctgg ccgcaccgtg aaagatatgc
     4321 tcaccgaggc catttcccgc accggtgaaa acatccagat cgcccgtttc acgcgcttcg
     4381 cgctgggtgg agaataagcc gacgcgctta aaaaaggtgt tgctgccatg acctataaac
     4441 cggaaaagat ccaccgcctg ctgctcaaga tcagcggtga ggtgctggcc ggccccaagg
     4501 gatttggcta tgatgacgcc gtcatagatg tcctcaccga cgagatcatc gaagcccggc
     4561 gcctcggcta cagcctcgcc atcgttttgg gcggaggaaa catctttcgc ggcggaacct
     4621 ggaaaaacca gagcctcaac cgcgttaccc tcgaccacat cggcatgctc gccaccgtgc
     4681 agaattccct ctatctggct gaaatcctga accgcaagaa ctattccgcc caggtattca
     4741 ccagcttcag cattgatagt gtggccatgc attacacgcc tcggcgggcc tttcaggcaa
     4801 tggaagccgg cagtatctgc ctgctcagcg gcggaacggg aaatccgttt ttcaccaccg
     4861 acaccgccgc ggtgctgcgt gcctgtgagc taaagctgga tatggttttc aagggcacca
     4921 aggtcgatgg cctctacagc gccgacccca aggttgaccc cacggccgaa ttcatatccg
     4981 atgccaccta tgaccaatgc ctggaacaaa aactgggcgt gatggacatg accgccttct
     5041 cgcttgccca agctaacgaa ctgccgatca agatcttcaa catcggcaaa cagggcgggc
     5101 tctgccaggc aatatccagc cccgaccacg gaaccttcat ccatccctga caggccaagg
     5161 acgaaagagg taaaaatgga acaactcatc tctcaatccc aagacaaaat gaacaagtct
     5221 ttcgatgctt tgctgcatca gttctccagg gttcgcacag gacgcgccag cgcttccatc
     5281 ctcgacgatg tgcgcatcaa ttattacggc agccccaccc cggtgaaaca actctgcaac
     5341 atctcgatcc ctgaaccccg caccattgtg gtgcagccct gggacaagac caccctcgcc
     5401 gacatcgaaa aggccatcct cgccgccaat ctgggcatca ccccggaaaa tgacggcaac
     5461 gtgatccgct tgcctttcca gccacttacc gaggagaaac ggcgcgacat cgttaaaaac
     5521 ctcaaaaaac tcgccgagga cgcccgagtt gccatccgca acattcgccg cgaagcaaac
     5581 gaccaggcca aaaagatgga aaaggacagc gaaatcagtg aggacgagca gaaaaagctc
     5641 ctcaaggata tccaggacct caccgacgaa tgggtgaaaa agatcgacga agtggagaaa
     5701 ggcaaggaaa aagagatcat ggaagtctga taagaacctt gatcacataa agatatgcgt
     5761 ccggcagcct ggctgccgga cttttttgtt acgtgaggcc tatgcaccct cgtacgctgg
     5821 attccgggtc gagcccggaa tgacgtgggt ttaggacagg gcggtggttg tttctggatt
     5881 ccgtcactgc atttcggaat gacgtgggtt cgggagtttc ggcgtttggg caagcgccgc
     5941 tacatgtgta gaggcggtcg ctgtaccgct tttttttgca aggggttgta tggttgagcc
     6001 gaatatacgt catcccggac gtcaccgagc ccgcgaggtg acattgatcc gggatctatg
     6061 aatggatgta cactggattc cgggtcgagc ccggaatgac gcgtaggaca ttggccggaa
     6121 gagccgaaca tacgtcatcc cggacgtcac cgagcttgcg aggtgacttt gatccgggat
     6181 ccatgaatgg atgtacactg gattccgggt caagcccgga atgacacggc ttggggtctg
     6241 gcctggtaac atctcacgaa caacccgctt acatctcctt gccgtggtgg gaagtaagcg
     6301 aattgttaag ggaatgttta ccggacacac tttagatact ttctataaat agtgggtaac
     6361 aaaacggggg ccggaaaaag agaaagagag aaaaggagaa aaagttggcg tggaggctgc
     6421 cgtcgagcga gaaacgggca tagacagcag caatggatga tttcccaaat acttcctgtc
     6481 gtccatgctc acttcgttcg ctgtacgcca ggtcaagtgc aggaatgacg gtaacgatgc
     6541 caggatttac ccttgataaa gtggtttctt cctggagacc aagcgggatt ttcgcgaaaa
     6601 agattgacgg aaagcaggcg attgagagta ttgtttataa caagacatgg aggttccgat
     6661 gatcaagatg agaactctgc tgccaatgct tctggtgctg agcctgggat ttggctgcgc
     6721 ctgcaacaag aaaaagaccg acatcacgcc ggtgagcgat gaagtgaaca tcagcctggg
     6781 cgaacagaac aacgctttcg ccttcaatct ttacgccttg gccgatgagc ccggaaaaaa
     6841 cctgttctat tcgccccaca gcatcacttc cgcgctgtcc atggtctatg gcggcgcgca
     6901 aggcaacacg gccaagcaga tggccaaagc catgagcttc aaccttcccg cggaagagca
     6961 gcacaagtca tatctttcac tgcaacagtc tcttaacgcg ctcggcgaga aaggcaaggc
     7021 cgagctgaac gtggccaacg ccctctttgg ctccaccacc aatgagaagt tcatcaggaa
     7081 agattatctg aagctgctgc gcgagtgcta tttgagcgac ctctacagcc tgaatttcag
     7141 cgatttcagc ggcacggcga aattcatcaa cacctgggtg gagggaaaaa ccaaagaccg
     7201 gatcaagaac ctggtgaccg aagaccacat ccgcgacagc aatgaaggca tggttctggt
     7261 gaacgccatc tatttcaaag gctcctggct caatgagttc gagcccaaac tcactatgaa
     7321 ggacaa
//
LOCUS       DAOOWR010000039         7219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10370_length_7219_cov_4.271636, whole genome shotgun sequence.
ACCESSION   DAOOWR010000039 DAOOWR010000000
VERSION     DAOOWR010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10370_length_7219_cov_4.271636"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..2631)
                     /locus_tag="PLB85_01855"
     CDS             complement(373..2631)
                     /locus_tag="PLB85_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU72100.1"
                     /translation="MFKRSLFFILLAVLSGLCLAKPVSERTQVRNLAAFTRLWGYVKH
                     WYPADEVQSIDWDRFAVYGSRQVLDARDDRELQARLLGLFSPLVPELQLFGKRAPDIV
                     PFEPVPGRSKVFWQYSGYNNTDSPSVYVSIRTNRPKMVRNIPGYDDAWTMLMPRLPQS
                     AEPETKARVTFRIRNLVPDSLTIKVYAGFEGDTVVDSLSGEGWEVKSFELESKGCDSE
                     FLWFGMAYFQHLQLDDVKIERWIDGDWQNVFETGFEDDVPGGLPQGFNLNILHDTMFG
                     QRTGFVVEDSPGGRLLSISKTPPEQEFTLILTDRFFAEQLPWGELLEKPLVRGLNCRF
                     PMVLQCDAERTYPVCDTTALNALGAELAGIDINDRGDRHVWLAGLIKYWNELSFFYPY
                     FEYGDCDWDGELPKALARVLRSRDFEQYKKALLLLMSQTGDGHSHLSDQSHNPNRPWF
                     TAIPLKGKWVVNKVLDPSLDLPPGSRITKMNGKNFARMMRENRPYFSSGNPESNDIYL
                     FSRSLRTYPDSVATFEFITPDRRKITRTVPFGDYEGWRYATPDAKFVNYPGGIVYVNP
                     NTITEEELQEALPELVAARGIILDLRYYLNISLDLLRYLLSAPDSLANSLVERYIRPR
                     EKLPHLNEDRPTWGVWPGEPHIGAEVVALCGRSSQSYCEAYLAVLKHNRRATLVGKPT
                     AGANGNVVITDLPGELKAYWTGMFVRQADKSRFHGVGIIPDILVETTLDDIVRGRDPV
                     LEKALELLREEN"
     gene            complement(2754..4061)
                     /locus_tag="PLB85_01860"
     CDS             complement(2754..4061)
                     /locus_tag="PLB85_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOU72101.1"
                     /translation="MNSDQTDFLAEQEQDLRDAPLAERLRPSSLDELRGQAKLVAKNS
                     LLNQMIASGEYHSFILWGPPGTGKTTIARIIERSSSHRFVQFSAVLSGIGDVKSVMKD
                     ADYAHKRRNQRTIIFIDEIHRFNKSQQDAFLPYVESGAVILIGATTENPSFEVIPALL
                     SRCHVFVLSMLSEEDLSAILAKGFAELGLKDNEAIRGWLAQQSGGDARRALNQLELLE
                     PWLKEDPQPDLKSLAAILEKKTMFYDKDREEHYNLISALHKSLRGSDPQAGLYWLARM
                     LEAGEDPRYVVRRLIRFASEDVGLADPNALLQAIAAKETVMFLGMPEANNALAQLVVY
                     LATAPKSNALYKAYSAAAEDARKTSHYGVPLHIRNAPTQLMKDLDYGKDYRYDHDNAD
                     HYAYQKYFPDQMPEKTYYTPGEFGFEKEIAKRLEWWLKLRRNK"
     gene            complement(4072..5394)
                     /locus_tag="PLB85_01865"
     CDS             complement(4072..5394)
                     /locus_tag="PLB85_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU72102.1"
                     /translation="MPGSKISGKSLDRWTASNILARMEKNRSKLWLALKYLRGQGHGF
                     INRSHWLTIAGITLGVTALICVTSVMNGLREDVRQRITGTLSEIKLSAPGGDPVPHPR
                     PLVDSLLALGYQAAPVIRSELILKQDDRIWSTLCFGIDPALHSKISAALHPRPSATGS
                     EVQGILSGGVEGPEFTDNGIALGYGLAAEINARVGNELQLLSPVFDVPTAFGMLPKVR
                     TVRVAAIFNAGMPEYNSTFSFVPLATAAFFRGYTDEVDYIEVKSPDASSPQRHTSRIR
                     SLFKGWQVEDWSAYDANLYSALRFEKFLMFVIMLFTYVIASFNLTGSMLKTIAQKKRE
                     LGLLKAFGYRETDLRDLFLYQSLILATIGIILGLVISTALLLVQKRFGLLSMGMGDAG
                     PLPLPVIFSFQDYLTVVLVSYFITLVSVILPLQRLKRIKPIELIRQTT"
     gene            complement(5446..7023)
                     /locus_tag="PLB85_01870"
     CDS             complement(5446..7023)
                     /locus_tag="PLB85_01870"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU72103.1"
                     /translation="MLIDKLKMSIRAVTRMLKPSRFLLLLMAVILLVEAVQPVGATIS
                     TRNKNYAEITRSMVETSRLYLGRPYQFRNPQGAIMDCSGFVQWIFSLHDVLIPRTSRE
                     QGASSRRIPLSDTRPGDLMFFRGTRGKSSVIGHVAMVVENTGETLKMIHSSSRGVVID
                     DYYSSPNYVKRFLYAGRMPQLEDKYPSHVPPTRNITVIGVGDIMLGTNYPSPQYLPPK
                     DGKELLAPVADILRSADLTFANLEGVILTDKGMPKQCSNPSTCYAFKSPDHYAQYLKD
                     AGIDVMSLANNHSGDFGVTGLRNTVMKLDSLGIRFAGRVEFPSKIFEKDGIIYGFAAF
                     APNTGTMQINDYAKVKQTVKELSEKCDIVIVSFHGGAEGADKTRVTRKTEFYLGENRG
                     NPHEFARVAIDAGADVVFGHGPHVTRAIDIYKGRFIAYSLGNFATYGRFNLSGKLGIA
                     PIVKLNLKSDGEFIDGEIISVKQPGKGGPVLDESRAALNEVINLTRLDFPESELLISP
                     EGKVTRKDAEPAISLNK"
BASE COUNT         1423 a         1867 c         2343 g         1586 t
ORIGIN      
        1 cgaggtgaca ttgatccggg atccataacg gaatatggac tggattccgg gtcaaacccg
       61 gaatgacgtg ggtttagggc tggtggtggt tgcttctgga ttccgaggca atctcggaat
      121 gacgtaggtt tggggtgaca ggcttattta ctgtcgtcgc tgcccgtgcc tcgctcgacg
      181 ccagcgtgta gccgtatgga agcaaatccg actggccatt aaggccctgt cctatgttcc
      241 attgtcgcct cccgcataat gcccgtattt gatgcgggta tcttgcgaga ggcaggggag
      301 gggtgtggac gcgcgggttc agggtctgac cagcagagca cagaggtgaa taaagattcc
      361 ctaggccggg ggttagtttt cctctctcag cagctccaaa gccttttcca gcacagggtc
      421 acgcccccgc actatgtcgt ccagggtggt ttccaccagg atgtccggga tgatcccaac
      481 tccgtggaag cggcttttat cggcctgacg cacgaacatt cctgtccaat aggccttcag
      541 ttcgccgggc aggtctgtaa tgaccacgtt tccgttggct ccggcggtgg gctttcccac
      601 cagcgtggcc cgacggttgt gtttcagcac ggccagatac gcctcgcagt agctttggct
      661 tgagcggccg cacagggcca ctacttccgc gccgatgtgc ggctccccgg gccagactcc
      721 ccaggttggc cggtcttcat tcaggtgggg caatttctcc cgggggcgga tgtaacgctc
      781 gaccaggctg ttggccaggg aatcgggagc ggacagcaaa taccggagca aatccagaga
      841 aatattgaga tagtagcgca ggtccaggat tatcccccgg gctgccacca gctccggcag
      901 ggcctcctga agttcctctt ccgttatggt gttgggattc acatacacga tcccgccggg
      961 gtaattgaca aacttggcat cgggcgtcgc ataccgccag ccttcataat ccccgaaagg
     1021 gacggtccgc gttatcttgc gccggtccgg cgtaatgaat tcaaaggtcg ccacgctgtc
     1081 cggataggtt ctcagggacc ggctaaacag atagatgtca ttgctttccg gatttcccga
     1141 ggagaagtaa gggcggtttt cccgcatcat tctggcgaag tttttcccgt tcattttcgt
     1201 tatgcgcgaa cccggaggaa ggtcgaggga agggtccaaa actttgttga ccacccattt
     1261 tcccttcagc ggaatggcgg taaaccaggg cctgttggga ttgtgggact ggtcgctgag
     1321 gtgggaatgc ccgtcgccgg tttgggacat cagcagcagc aaggccttct tgtattgctc
     1381 gaagtcccgg ctgcgcagca ccctggccag cgctttgggc agttcgccat cccagtcgca
     1441 gtcgccatat tcaaaatagg gataaaagaa actaagctca ttccagtatt tgatcagccc
     1501 cgccagccag acatggcggt caccgcggtc gttgatgtct atgccggcca gttcggcgcc
     1561 cagggcgttg agggcggttg tgtcgcagac cggataagtg cgttcggcat cgcattgcag
     1621 gaccatggga aagcggcagt taaggccccg gaccaggggt ttttccagca gttcgcccca
     1681 gggaagttgt tcagcgaaga accggtctgt gagtatgagc gtgaattcct gctccggcgg
     1741 ggttttgctg atggagagca gacgtccccc ggggctgtct tccaccacaa accccgttct
     1801 ttggccaaac atggtatcgt gcaggatatt gaggttaaag ccctggggca ggccgccggg
     1861 aacgtcatcc tcaaaacctg tctcaaaaac gttttgccag tcgccgtcga tccagcgttc
     1921 gattttgacg tcgtcaagct gaaggtgctg aaaataagcc attccaaacc agaggaattc
     1981 agagtcgcag cctttggatt ccagttcaaa gcttttgacc tcccagcctt ctccggagag
     2041 ggaatccacg accgtatcgc cctcaaaacc ggcatagacc ttgatggtga ggctgtcggg
     2101 cacaaggttg cggatccgga acgtgacgcg ggctttggtt tcaggctcgg cggactgggg
     2161 caatctgggc atcagcatgg tccaggcatc atcgtatccc ggaatgttgc gaaccatctt
     2221 tggacggttg gtgcgtatgc tgacatagac gctggggctg tcggtgttgt tgtagcccga
     2281 atactgccaa aagaccttgg agcggccggg gacgggttcg aaggggacaa tgtcgggagc
     2341 gcgcttgcca aagagttgta gctccggcac cagcgggctg aagaggccaa gcaacctggc
     2401 ctggagttca cggtcgtcgc gggcgtccag cacctggcgg gaaccgtaaa cggcaaagcg
     2461 gtcccagtcg atggactgga cttcgtcagc gggataccag tgttttacat agccccagag
     2521 gcgggtgaag gctgcaaggt tgcgcacctg ggtcctttcg gataccggtt tggccaggca
     2581 caatccggaa aggacggcca gcaggataaa aaacagagaa cgcttgaaca tgatttactc
     2641 cttttcccaa gcgatttggc caggctggaa ccttgcacaa gggatacgct tccgggaatt
     2701 tgttgagttg attctaaagc tggagagcgg cagctcgcca atcggagcca taatcactta
     2761 ttccgccgaa gcttaagcca ccactcgagg cgtttggcga tctctttttc aaagccgaat
     2821 tcgccgggcg tgtaataggt cttttccggc atctggtccg ggaaatactt ttggtaggca
     2881 tagtggtcgg cgttatcgtg gtcgtagcgg taatccttgc cgtaatcgag gtctttcatg
     2941 agctgggtgg gggcgttgcg aatgtggagc ggaacgccgt agtggctggt tttgcgggca
     3001 tcttcagcag ccgcggaata ggctttgtaa agggcgttgc tcttgggggc ggtggcgagg
     3061 taaaccacca actgggcaag ggcgttgttc gcttccggca tgcccaggaa catcacggtt
     3121 tccttggcgg cgatggcctg gaggagggcg ttgggatcgg ccaggcccac atcctcggag
     3181 gcgaaacgga tgaggcggcg caccacgtag cgggggtctt cgccggcctc cagcatccgc
     3241 gccagccagt agagcccggc ctggggatcg gagccgcgga gggatttgtg gagggcggag
     3301 atgaggttgt agtgttcctc gcggtccttg tcgtaaaaca tggtcttctt ttcgaggatg
     3361 gcggcgaggg atttgaggtc gggctgggga tcctctttca gccagggttc cagcagttca
     3421 agctggttga gggcgcggcg ggcgtcgcct ccgctctgct gagccagcca gccccggatg
     3481 gcttcgttgt ccttcaaacc cagttctgcg aagcctttgg ccaggatggc ggagaggtct
     3541 tcctctgaga gcatggagag cacaaaaacg tggcagcggg agaggagggc cgggatgacc
     3601 tcgaaggagg ggttttccgt ggtggcgccg atgaggataa ccgccccgct ctccacatag
     3661 ggcaaaaagg cgtcctgctg ggatttgttg aagcggtgga tctcgtcaat gaagatgatg
     3721 gtgcgctggt tgcggcgttt gtgggcgtag tcggcgtcct tcatcaccga tttcacgtcg
     3781 ccgatcccgg aaagaacggc gctgaactgg acgaaacggt ggctggaaga gcgctcgatg
     3841 atgcgggcga tggtggtttt ccctgttcct ggcgggcccc agaggatgaa ggagtggtat
     3901 tctccggagg cgatcatctg gttgagcagg gagtttttgg ccaccagttt ggcctggccg
     3961 cggagttcat ccaggctgga aggacgcagc ctttcggcca ggggggcgtc gcgcaggtct
     4021 tgctcttgtt cggccaggaa atcggtctga tcgctgttca cggtgtgtcc ttcaggtggt
     4081 ttggcggatt agttcgatgg gtttgatccg tttcagcctt tgcaaaggca ggatcacgct
     4141 tacgagggtg atgaaatagg aaacgagaac cacggtgaga taatcctgga aggagaatat
     4201 caccggcagg ggcagcggcc cggcgtcccc catgcccatg gagagcaggc cgaagcgttt
     4261 ctggacgagg agcagggccg ttgatatgac caggcccagg atgatcccga tggtggccag
     4321 tataagcgat tgatacaaaa agaggtcgcg caggtccgtt tcccggtagc cgaaagcctt
     4381 gagcagacca agctcccgct ttttctgggc gatggtcttg agcatggagc cggtgaggtt
     4441 gaagctggcg atcacatagg tgaaaagcat gatcacgaac atgaggaact tttcgaagcg
     4501 gagggcgctg taaaggttgg cgtcgtaagc gctccaatcc tccacctgcc agcctttgaa
     4561 gaggcttctg atcctgctcg tgtggcgctg gggtgaactg gcgtcggggc tcttcacctc
     4621 gatgtagtcc acctcgtccg tgtagccgcg gaaaaaggcg gcagtggcca gcggcacgaa
     4681 gctgaaagtg gaattgtatt caggcatgcc ggcattgaag atggcggcca cgcggacggt
     4741 gcggaccttg ggcagcatgc cgaaagccgt gggaacgtca aaaaccgggg aaagcaattg
     4801 cagttcgttg cccacgcggg cgttgatctc ggcggcaagg ccgtagccca aagcgatgcc
     4861 gttgtccgtg aattccgggc cctccacccc tccggaaagg atgccctgaa cctcggagcc
     4921 ggtggcggac ggccggggat gcaaagccgc gctgatcttt gaatggaggg cgggatcgat
     4981 cccgaaacag agggtggacc agatgcggtc atcctgcttg agaatgagtt cgctgcggat
     5041 caccggggcc gcctggtagc cgagggcgag caggctgtcc accagaggtc ggggatgggg
     5101 cacaggatcg ccgccgggcg cgctgagctt gatttcgctg agcgtgcccg tgatccgctg
     5161 gcgcacgtcc tcacggaggc cgttcatcac gctggtgacg cagatgaggg ccgtcacacc
     5221 gagggtgatc cccgcgatcg tgagccagtg cgagcggttg atgaagccgt gaccctggcc
     5281 gcgcaggtat ttcagcgcca gccacagttt gctgcggttc ttttccatac gggcgaggat
     5341 attggaagcg gtccagcggt caagagattt tccgcttatc ttgcttccgg gcaaagaaaa
     5401 agccggcttc gcgccggctg aaaggttgta ttatcggaaa gcggattatt tgttgaggct
     5461 gatggcaggt tccgcgtcct tgcgggttac cttgccttcg gggctgatca gcagttcgct
     5521 ttccgggaaa tcgagtctgg tgaggttgat gacctcgttg agcgcggccc tgctttcatc
     5581 gagcacgggg cctcccttgc cgggttgttt cacggagatg atctcgccgt cgatgaattc
     5641 gccgtcgctt ttgagattca gcttcacaat gggagcgatt cccagcttgc cggagaggtt
     5701 gaagcggccg taggtggcga agttgccaag gctgtaggcg atgaagcggc ccttgtatat
     5761 gtcgatggcg cgggtgacgt ggggtccgtg gccgaaaacg acatccgctc cggcatcgat
     5821 ggccacgcgg gcaaattcgt gtggattgcc gcggttctca cccagataga actccgtttt
     5881 gcgggtcacg cgggtcttat ccgcgccttc ggccccgccg tggaaggaga ctatcacgat
     5941 gtcgcacttt tcgctcagtt ctttcactgt ctgtttcact ttggcatagt cgttgatctg
     6001 catggttccg gtgttggggg cgaaagcggc aaagccgtag atgatgccgt ctttttcaaa
     6061 gatcttggag ggaaattcca cgcggccggc gaacctgatg cccagggaat ccagcttcat
     6121 caccgtgttg cgcaggccag tgacgccaaa atcgccgctg tggttgttag ccaggctcat
     6181 cacgtcgatc ccggcgtctt tcaggtattg ggcatagtgg tccgggctct tgaaagcgta
     6241 gcaggtggag gggttggagc attgcttggg catgcctttg tcagtgagga tcaccccttc
     6301 gaggttggcg aaggtgaggt cggcgctccg gaggatgtct gccaccggcg ccagcagttc
     6361 cttgccgtcc ttgggcggca gatactgggg cgagggataa ttagtgccca gcatgatgtc
     6421 gcccacgccg atcacggtaa tgttgcgggt gggaggcacg tggctgggat atttgtcctc
     6481 aagctggggc atgcgtccgg catagaggaa gcgcttgacg tagttggggc tggagtaata
     6541 gtcatcgatc accactccac gggaggagct gtggatcatt ttcagggtct cgccggtgtt
     6601 ttcaaccacc atggccacat gtccgatcac gctggatttg cctctggtgc cgcggaaaaa
     6661 catcaggtcg ccgggacggg tgtccgaaag cggtatccgc cggctgctgg cgccctgctc
     6721 cctggaggtg cgggggatga ggacatcgtg gagggaaaag atccactgca caaaaccgct
     6781 gcagtccatg atggcgccct ggggattgcg gaactggtaa ggacggccaa gatagaggcg
     6841 gctggtttcc accatgctac gggttatctc agcgtaattc ttgttgcggg tgctaatcgt
     6901 ggctcccaca ggctggaccg cttcgaccag caggatcacc gccatgagta aaagcaggaa
     6961 ccggcttggt ttcagcatcc gggtcactgc gcgaatggac attttcagtt tatctatcaa
     7021 cataaattct ccaatgtggc atgcgtcccc ggcgcatatt cagaagcacg ttcggatggg
     7081 gacactcaat cactatgttt acaattggtt gcaaataaag gttcaatcat agcagaaggg
     7141 ggacgggtgt caaggaaaaa actggcctga gagacaggca ctattcctat ctaattgtat
     7201 tgctgtcgat tgcggcgat
//
LOCUS       DAOOWR010000040         7198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10402_length_7198_cov_3.288534, whole genome shotgun sequence.
ACCESSION   DAOOWR010000040 DAOOWR010000000
VERSION     DAOOWR010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10402_length_7198_cov_3.288534"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLB85_01875"
     CDS             <1..782
                     /locus_tag="PLB85_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOU72104.1"
                     /translation="EAGKFYLRRMDESTGIPERMIMETAIQSMGLAELGPFDLDKKVI
                     EYAIARSDSLCSLSLESFADLLSTDSPAPGGGSVAALCLSLSGALSAMVANLTVDKKG
                     YESVQEAVRELAPRGQEIKLQALQCIDADTDAFNAMMEAMRLPKKTDEEIAVRNAEME
                     RTTQQAILVPFRTLELAWEAMQLADKVADVGNANAISDAGVAALTALSAAKAADYNIL
                     INLPGSTDEAFKADIKTRAGELVSKCQTLADAIETKIRNRF"
     gene            848..1231
                     /locus_tag="PLB85_01880"
     CDS             848..1231
                     /locus_tag="PLB85_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72105.1"
                     /translation="MLTTLVALYSILVALNLLDGFSTWLVLKPNHYHRERNPAARWMF
                     IKLGLTRGLVLAEVLWIGFISAVFFLTFTHPVWNVVLLVLLCVGVLVFLRVVSGNFRA
                     WRRIRQREELLAGKQQKEEADVGHA"
     gene            1215..1595
                     /locus_tag="PLB85_01885"
     CDS             1215..1595
                     /locus_tag="PLB85_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOU72106.1"
                     /translation="MLAMLENVFSQVAVLAGQFVVFVILNILDGHSTWKVIQPNHLHR
                     ERNPVARWIFRKLGLPRGIVIFKAVLLFILGGAFAVYAKKETKMMNITLGVANLVFLA
                     VVIHNYKVYKRLKARRDIRREFLP"
     gene            1592..3106
                     /locus_tag="PLB85_01890"
     CDS             1592..3106
                     /locus_tag="PLB85_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72107.1"
                     /translation="MKLWQLLLILFALALPLAAQVNGNLQYIGDKAILQVWGDHYQRG
                     YAQGYLLAQPTLDVFDEFFYMMFVFSDPARYAYLWNYYQEHFNTDARTLQEAQGLVAG
                     IAASGASLYHSGLQRDITHDDILLANAFLDLQQLRNDLGGPDIDLGCASLSSWGISTQ
                     QDSLLTGSSVITRWLDWTLFECLIDNPLLVVHHPSEPDEQKWLSFTFPGWLGAASAIS
                     ESGTWASLNVCNDHSVVNQSGLDPIFFDIRRGLERTDLNGDGVSNIMDVAAAIQAGNN
                     LTGTIIHSLSESAGQTISVVIENNNTGTALRYYDDPSSNLPGQNLAATNHFRLLTFPT
                     CCTRYANIADSLNMNHHITAKRQWSLLSGAAGQETTLTALQYTPSTGSILWAGAAVSL
                     PANQNPAIHLSASDLFSFSTPVTDELLPSHQFAFSCAPNPVSAAQTLRLSGSQPLKSL
                     ALYNLRGQKILDLSFSGNKSDAEISLPALPAGVYLAWVQGVSGSSAQSKLLVLP"
     gene            3355..>5718
                     /locus_tag="PLB85_01895"
     CDS             3355..>5718
                     /locus_tag="PLB85_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72108.1"
                     /translation="MNKKVLFICLLVWLTLGLQAQLSWPEPLTLADNNNISFLGQMVR
                     TTQGNTVLIYSQYDGEGTHTYMQAYTQDTQTMWDNPIELSSILSLSYIFLTNDSILNV
                     FTPKYNGYNYNLVLNRYTLNGERVGVLDRPVMGNYVNRVEIYSDFQGGMHVFGKNYNT
                     SNYQHINAEGIPQHPITDESVFSWLPSETHFPIKYVCGDGSLLMSYTSGNNGGIFRMD
                     SSLNVLFSHSFTTADSEGINTSLVQRSDDGFYLMRAKGSVVRLYSYNSNGELLWPEPV
                     IINQYTYCEAACDSQDRLITCYLSSHYIYSQVYDTQGNSIFDEPGIHIHTFPSNTTFE
                     SPVISVMPNGANGFYLIRRYTDTVWDEDFLQYIDLQTANSMWIHSLGDLETNMGSTYY
                     ATIEDGVFKFWNNFRTGNRSGITYNGINEAGLPEYASPGAFLESGFFGEVDMIRIRPI
                     GPDKAIAVWAEVEPNYPNPKTPKYNLIHPGGTWDFEDGQALISTPRDILYLDCFSTDD
                     DCALVVWQVNSSNVYVQKIDRFGLTLWPQPKNLFTGLPSAVRASYYENSLYLSYMHPS
                     GTNIVMQRFVEGEPQWGPDGVMVAGTANGNPVLIDLIDRTVIWGVVANEDTYSMFYFN
                     RVDQNGQSLEGFSINGLPITEAQAGDPLLRVHRVIPGDGRLWFELSMGHSEYINDGHS
                     NGYWDIVFTPVVQSVSYDGDILFPNGGISGSYSVGSMADETGYYKCTACSKHLRITKY
                     GLDGNIAWESNPLTEVNWLLDFVFNYGVSLQKVSDTEILAHAVTYTGN"
     assembly_gap    5719..5728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5729..6202
                     /locus_tag="PLB85_01900"
     CDS             <5729..6202
                     /locus_tag="PLB85_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72109.1"
                     /translation="ILFSPEGQFTYPADMYIQSCPNTGSIGSVTHTRMGTYLLMMNTN
                     GSYAEIQFRASGAELSPDGNVPVLKLISAYPNPFRQELILDFAIERSDNARLEIFNIR
                     GQRVLQKDYNELLQGKNKFVWNGRDELNRECASGIYFIRMMDGKHRDTLRTVRLK"
     gene            6674..>7198
                     /locus_tag="PLB85_01905"
     CDS             6674..>7198
                     /locus_tag="PLB85_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU72110.1"
                     /translation="MKILITGGAGFIGSHLAERLLEDGHKVSIVDNLSTGRLENIESI
                     KENGNFHYTIGSILNRSLMEKLMAGIDQVYHLAAAVGVKYVIENPLLSLKTNIVGTDN
                     VLELANKHKAKVLITSTSEIYGKSDKVPFSEDDDRLLGSTRISRWGCSDSKAIDEFMA
                     LACFRDKRLSVVIVR"
BASE COUNT         1791 a         1856 c         1777 g         1764 t
ORIGIN      
        1 tcgaggctgg aaagttctat ctgcgccgga tggatgaaag caccggcatc ccggaaagga
       61 tgatcatgga aaccgcgatc cagtcgatgg gcctggcgga actgggacct ttcgacttgg
      121 acaagaaagt tatcgaatac gccatcgcaa ggtccgacag cctttgctcc ctgagcctgg
      181 aaagctttgc cgatctgctt tccaccgatt cccccgcccc cggtggcggc agcgttgccg
      241 cgctctgcct ttccctttcc ggagcgctgt ctgcgatggt ggcaaacctc accgtggata
      301 aaaaaggcta tgaatcagtt caggaagctg tgcgcgaact ggctccccgc ggccaggaga
      361 taaaacttca agccctgcaa tgcattgatg ccgacacaga tgccttcaac gccatgatgg
      421 aagccatgcg cctgcccaaa aagaccgatg aggaaattgc cgtccgcaac gccgagatgg
      481 aacggaccac ccaacaggcg atcctggttc ccttccggac tcttgaactg gcttgggaag
      541 ccatgcaact agctgacaag gtggccgacg taggaaacgc caacgccatt tcagacgctg
      601 gtgtggcagc cctgaccgcg ctcagcgcgg ccaaagcggc ggactacaac atcctgatca
      661 acctgcccgg cagcactgat gaagctttca aggccgacat caaaacccgc gccggagaac
      721 tggtgagcaa gtgccagaca ttggccgatg ccatcgaaac caagatcagg aaccggttct
      781 gaacccagtt gagctaaacc cggccataag agccagcctg acctgataac cacggagccc
      841 aagcccgatg ctgacaaccc tggttgccct ctactccatc ctggtggcgt tgaacctcct
      901 ggacggattt tccacctggc tggtgctgaa gccaaaccac tatcaccgtg agcgcaatcc
      961 cgctgcccgc tggatgttca tcaagcttgg cctcacccgg ggccttgtct tggctgaagt
     1021 gctgtggatc gggttcatca gcgccgtctt tttcctcact ttcacccatc ctgtctggaa
     1081 cgtggtcctg ctggtcctgc tctgtgtggg agtgctggtt tttctcaggg tcgtgagtgg
     1141 aaatttccga gcctggcggc gcatccggca gcgggaagag cttctggctg gaaagcagca
     1201 aaaggaggaa gcggatgttg gccatgcttg agaatgtttt tagccaggtc gcggttttgg
     1261 caggacagtt cgtggtcttt gtcatcctta atatccttga cggccattcc acctggaagg
     1321 ttatccagcc caaccatctg caccgtgagc gaaaccccgt cgcccgctgg atctttcgca
     1381 aacttggcct gccccggggg atagtgattt tcaaggccgt gctcctcttc atcctgggtg
     1441 gcgcttttgc tgtttacgcc aaaaaggaaa ccaagatgat gaacataacc ctcggcgtgg
     1501 ccaacctggt cttcttggcc gtggtgatcc acaactacaa ggtttacaaa aggttgaagg
     1561 cccgacgcga catccgcagg gagtttttac catgaaactc tggcaattgc tgctgatcct
     1621 cttcgctttg gctctgccgc tggccgctca ggtgaacgga aacctgcaat acatcggaga
     1681 caaagccatt ctgcaagtat ggggcgacca ttaccagcgc ggctatgccc agggctattt
     1741 gcttgcccag cctacacttg acgtattcga tgaattcttt tacatgatgt tcgttttttc
     1801 agaccccgcc cgctatgcct atctctggaa ctactatcag gaacatttca acaccgatgc
     1861 gcgcacgctg caggaagccc agggactggt tgcggggata gctgcttcag gcgcaagcct
     1921 ttaccacagc ggcctgcagc gcgacatcac tcatgacgac atacttttgg ccaatgcttt
     1981 tttggacctc caacaactcc gcaacgacct tggcggcccg gatattgatc tcggctgcgc
     2041 gtctctctcg agttggggaa tctccaccca gcaggattcg ctgctgaccg gctcttcagt
     2101 gatcacccgc tggctcgatt ggaccctgtt tgaatgcctg atcgacaatc ccctcctggt
     2161 ggtgcaccat cccagcgaac cggatgagca aaaatggctc tccttcacct tcccaggttg
     2221 gctgggagca gcttctgcca tctccgaatc cggcacttgg gcttccctga atgtgtgcaa
     2281 tgaccactct gtggttaacc agagcgggct cgaccccatt tttttcgaca tccgcagagg
     2341 cctggaaaga accgacctga acggagacgg agtttcgaac atcatggacg tggccgccgc
     2401 catccaggca ggcaacaacc tcaccggcac catcatccac agcctcagcg aaagcgcggg
     2461 acagacaatt tctgtagtga tcgaaaacaa caacaccggc acggctctgc gatattacga
     2521 cgatccaagc agcaaccttc cgggacaaaa ccttgccgcc accaaccatt tccgcctgct
     2581 taccttcccg acctgctgca cccgctacgc caatatcgcg gattccctga acatgaacca
     2641 ccacatcaca gccaagcgcc aatggtcttt gttgtcaggt gccgcgggac aggaaacgac
     2701 actcaccgcg cttcagtaca ccccctccac cggcagcatt ttgtgggcag gcgccgcggt
     2761 ttcccttccc gccaaccaga atcccgccat ccatctttcc gcgtcagacc tgttcagctt
     2821 ttccacccct gtgacggatg aacttctgcc cagtcaccag ttcgcttttt cctgcgcccc
     2881 aaatccagta tcggccgccc aaacgctcag gctgagcggc tcccaacctc tcaagtccct
     2941 tgccctttac aacctccgcg gtcagaaaat cctggaccta agcttttccg ggaacaaatc
     3001 cgatgctgag atttcccttc ccgctctgcc cgcgggtgtg tatctggctt gggttcaagg
     3061 cgtttcgggc tccagcgccc agagcaaact gctggtctta ccctgaaccc ccgaggattc
     3121 gcctaagatt cgatccccgt ataatgcccg catcaaatac ggggatttgg cgggcggaag
     3181 acgggggacg gacaacatgg ggctaaggaa gtgccggaaa actggtttgc ccttaaacac
     3241 cagcttgttc tgatccagat gattcctgat atccatctca tcacaaccta acaatcttct
     3301 tgacaaatat agaacttaac ttaacttggg aaattagaaa atggggagga tagtatgaat
     3361 aaaaaagtcc tgttcatctg tctcttggtt tggctgacgc tggggctcca ggctcaactg
     3421 agctggccag aaccgctgac actcgctgat aataataata tcagcttctt aggccaaatg
     3481 gtgcgaacca cacaggggaa caccgtcctg atctactctc aatatgatgg tgaaggaacc
     3541 catacttaca tgcaggctta cactcaggat actcagacca tgtgggacaa tcccatagaa
     3601 ttgagtagca ttctgagttt aagttacatc ttcctgacca atgattccat tcttaacgta
     3661 tttaccccca agtataacgg gtataattat aacctggttt tgaacagata cactctgaac
     3721 ggtgagcggg tgggagtttt ggacagaccc gttatgggaa actatgtgaa tagagttgag
     3781 atctattccg actttcaggg agggatgcac gtcttcggca agaactataa taccagtaac
     3841 tatcagcata ttaacgcaga aggaatcccg caacatccca ttactgatga aagcgtcttt
     3901 agctggttac cttcggaaac ccactttccc attaaatatg tgtgcggtga cggctccctg
     3961 ctgatgagtt atacatccgg aaacaatggt ggaatttttc gcatggacag ctctctcaac
     4021 gtcctcttct cacatagctt tactactgcc gatagcgaag gcattaatac atctctggtt
     4081 caacggagtg atgatggatt ctatctaatg cgcgccaagg gaagcgtcgt caggctctat
     4141 tcttataata gcaacggtga attgctctgg cctgaacctg tgataatcaa tcaatacacg
     4201 tattgtgaag ctgcatgtga cagtcaggat cgcctgataa cctgttatct cagtagtcat
     4261 tacatataca gccaggtcta cgatactcaa gggaatagca tctttgatga acccggtatt
     4321 catatacata catttccttc caacacaacc tttgaatcgc cggtgatcag cgtcatgcca
     4381 aacggtgcca acgggtttta tctgatcagg agatatactg ataccgtttg ggacgaagac
     4441 tttctgcaat acatagattt gcaaactgcc aatagtatgt ggatccatag tttaggtgat
     4501 ttggaaacca atatgggctc tacttattat gcaaccatcg aagatggcgt tttcaagttc
     4561 tggaataact ttcgcaccgg aaatagatcc gggattactt acaatgggat aaatgaagcg
     4621 ggactccctg aatatgccag tcccggcgcg tttttggaat caggattctt tggcgaagtg
     4681 gacatgataa gaatcagacc aatcggacca gataaggcca ttgctgtctg ggcagaagtt
     4741 gaaccgaatt atcctaatcc gaagactccg aaatataatc tgatccatcc tggaggaact
     4801 tgggattttg aagatgggca agcgcttatc tccactccca gggatattct ctacttggat
     4861 tgcttcagca cggatgatga ttgtgctctg gtggtttggc aagttaacag cagcaatgtt
     4921 tatgtgcaga agatcgatcg ttttggttta actctgtggc cccagccaaa gaatcttttc
     4981 accgggctgc cgagcgcagt gagagcttct tattatgaga attcgctcta cctctcatat
     5041 atgcatccca gtggcaccaa tatagttatg cagcgttttg tggagggaga gccacagtgg
     5101 ggtcctgatg gggtgatggt ggcaggtact gccaatggca accccgtcct catagactta
     5161 atagacagaa ccgtgatctg gggagtggtg gcaaacgagg acacgtactc aatgttctac
     5221 tttaacagag tggatcaaaa tggacaaagt ctggagggat tcagtatcaa cggcttaccg
     5281 atcacagaag cacaagccgg ggatcctctt ctgagagttc atagagttat tcctggagat
     5341 ggcagattgt ggtttgagct ttccatggga cattctgaat acattaatga cgggcacagc
     5401 aacggatact gggacatagt atttacgcca gtcgtgcagt ccgtgtctta tgacggcgac
     5461 attctcttcc ccaatggagg gatatccggt tcgtatagcg ttggcagcat ggcagatgaa
     5521 accggttatt acaagtgtac tgcttgttcc aagcatctca ggattactaa atatggcctg
     5581 gatgggaaca tcgcctggga gtccaatccg ctgactgaag tgaactggct tttggatttt
     5641 gttttcaact atggagtgag tctgcagaaa gtgtctgata ccgagatttt ggctcatgca
     5701 gtcacctata caggtaacnn nnnnnnnnat cctcttttct cccgaaggac aattcaccta
     5761 tcctgctgat atgtacatcc aaagctgccc aaatactggt tctatcggat cggtgaccca
     5821 tacccgaatg ggaacctatc tcctgatgat gaataccaat ggatcctatg ctgagataca
     5881 gttccgggcc tcgggcgcag aacttagtcc cgatgggaac gtccctgttc tcaagttgat
     5941 ctctgcttat cccaatccat tcagacagga attgattctg gatttcgcta ttgaacgcag
     6001 tgacaacgcc aggctggaga tctttaatat ccggggacag cgtgtcttgc agaaggatta
     6061 taacgagctt ctgcagggaa agaacaagtt cgtctggaac ggcagggatg agctcaaccg
     6121 tgaatgtgcc agtggaatct atttcatcag aatgatggat ggtaaacaca gagatacctt
     6181 gaggactgtt agattgaagt gagggagtag gtttcaccac agcgaaccca gaaaaaagaa
     6241 gagattattt agaagtgagt aaatcacggc ttcgcttatt gcaatgtgag aaaagtttta
     6301 ggttcttctg ttttatcgga cttggacttt cctgtgttag tgacccattt tcatcgctaa
     6361 tacttcaaag ctactccttt tttgcaggca tttggatatt ccccatgggt tagcagaatc
     6421 cagaacaatg tgcacaataa aatgctctga aagaagttag tatatccata gcaatcaact
     6481 cgtctggatc ataatctttg ggactcagac atttgaagtg ttggtgtttg gatttgcatt
     6541 tttctgtgag ggcttgaact gaaagcgttc caaatcctct tttctattga caaaaacccg
     6601 gctggcagat tttgttacgt aatggcaaaa tatgtttgtc atataggata aagacttcaa
     6661 atctggagaa gttatgaaga ttctgatcac cggcggggcc ggttttattg gctcgcattt
     6721 ggcggaaaga ttgttggaag acggacataa agtaagcatc gttgacaatc tttccaccgg
     6781 ccgcctggaa aacatagaat cgattaagga aaacggaaat ttccactata cgatcggcag
     6841 tatcctgaac cgcagtctaa tggaaaaact aatggccggg atcgaccaag tttatcatct
     6901 ggcagcggcc gtgggcgtga agtatgtcat cgagaacccg ctactttcgt tgaaaacgaa
     6961 catagtgggc acggacaacg ttttggagct ggcgaacaag cataaggcca aggtgctgat
     7021 cacctcgacc tccgaaatct acggcaaaag cgataaagtt cccttttcag aggatgacga
     7081 ccgcctgctt ggctcaaccc gcatcagccg ctggggttgc agcgacagca aagctatcga
     7141 cgaattcatg gctctggctt gtttccgcga caaaaggctg tcagtcgtga tcgtgcgc
//
LOCUS       DAOOWR010000041         7183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10428_length_7183_cov_3.780022, whole genome shotgun sequence.
ACCESSION   DAOOWR010000041 DAOOWR010000000
VERSION     DAOOWR010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10428_length_7183_cov_3.780022"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLB85_01910"
     CDS             complement(<1..813)
                     /locus_tag="PLB85_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72111.1"
                     /translation="MLTAVVFLLFLSGCHKPTELEQVSKPVFSPPGGTYDSAVEVVIS
                     TATTGATIRYTTNGADPNSSSPVYSSPITVSQNATLKAKATKSGWTDSSITTAEYTIS
                     PIQTVATPTFSPPGGSYTSAQSVLISCSTSGAVIRYTTNGAEPTESSPTYSNPINVSS
                     TTTIKAKGFKNGWTPSATASETYTISAPPPPPVQMIYVPGGTFTMGRTTGSGYSDELP
                     THMVTLNSFYIGKYEVTQAEYSQYMQPGYSWTSDYGLGDNYPAYNVSWYAILK"
     gene            1536..2069
                     /locus_tag="PLB85_01915"
     CDS             1536..2069
                     /locus_tag="PLB85_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72112.1"
                     /translation="MKKLYVIMILALAAVGLCAQEGLFGLAYGDDLNFADSLMVQQGF
                     IAREVISSWVIYTSDYNPLIDFVVLYVDPATEILRGWSVVYNAENTQEQDGYVLDRLH
                     KMHGENVLVNEARETVSWIFDENLAASYSYSPQGNLRVMYYDFSCPELFALPGTEKEP
                     EPSEPAEPDVEEKNQAR"
     gene            2202..3263
                     /locus_tag="PLB85_01920"
     CDS             2202..3263
                     /locus_tag="PLB85_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU72113.1"
                     /translation="MKTGGCRFGTHRVIEPKGVLPQPARVLNNDMSEIWDNELLIDVI
                     RLNVDSASFHQIKNKLIAQGHTDLEKAFADHAIDLVNRTGKHKNEDTGSGGMLIGRVA
                     QIGPKFEMKDEIAVGDTIASMVSLSLTPLKINKVKRVLLDKDQVEIEGQAILFSSGIY
                     AKLPDDMDENLALSVLDVAGAPAQVERLARPGDTVVVLGANGKSGILCNAIARERVGV
                     SGKVIGIVRNPAYIETCKNTGCHEVILASATDAITIQKEVARLTNGRMADVVINVVNI
                     EGTELPSIMACRERGLVYFFSMATSFTKAALGAEGIGADVDMILGNGYARHHAAISLD
                     LLRRNPVLMKLFQERYTDK"
     gene            3272..4519
                     /gene="ablA"
                     /locus_tag="PLB85_01925"
     CDS             3272..4519
                     /gene="ablA"
                     /locus_tag="PLB85_01925"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOU72114.1"
                     /translation="MAELTDIRSIATEQEWNDWRWQIRNRITDFETLKKYIELQPEEE
                     AVASSDSFSFRMAITPYYLSLIDHKNPNDPVRLQAIPRIAESVTGPSDMADPLNEDAD
                     APVPGMTHRYPDRVLLLLTDQCSMYCRHCTRRRKAGEYDAPMPKENVDKAIKYIEEHK
                     EVRDVILSGGDPLTMSDARIDEVLVRLAKIDHVDIVRIGTRTPVVMPQRITDELITML
                     KKYKFVWLNTHFNHPHEITPDSAAALAKLAEAGVLLGNQSVLLKGVNDHVDVMKSLVH
                     ALVRNRVRPYYIYQCDLSEGISHFRTPISKGIEIIESLRGHTSGLCIPTFVVDAPGGG
                     GKIPVMPNYIISQMPGRVVLRNYEGFISAYTEPEFAPLDESKYQSRCAPERQSTEGVM
                     TLLRGKKVSIGPADTRRNQRRTK"
     gene            4533..5564
                     /locus_tag="PLB85_01930"
     CDS             4533..5564
                     /locus_tag="PLB85_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72115.1"
                     /translation="MKHGAWPGPFDWIQAAEPRAVAIVGICKNAGKTTLLNHLLSLRK
                     KGVWGVFSTGIDGEERDSVFRVPKPAVKLGPDIIFCCDTANLDALGSGVKVLEKVRGS
                     SAARPLWLARSLAPLQTEITGPGSVSEQISVLQTMWEQGAGKVLIDGSLDRKSIALSE
                     AVDAVAVVIGASFGSVSSVITEIRRLELLNSLPEAKLGQQEFAKLLGSEKILLKKDGC
                     WTGSDLHSLLGNEKELKKLLEQGHEAIYIPGALTDAVFPALREPLAALKTNIIFRHPE
                     CLKLGLTRLERLVEELQPQVLIPFRIWSWTVNTASVGNVAVDAADFRARLREAFPQLD
                     LPDIRELTS"
     gene            5561..6460
                     /locus_tag="PLB85_01935"
     CDS             5561..6460
                     /locus_tag="PLB85_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU72116.1"
                     /translation="MSDRDKITNRTVNFGLFTNVVLAVLKSLIGIIGHSQALLADGIN
                     STSDVVYYVAVKIFMRQANKPADPEHPYGHRQLESISAIVVGAFIITTGIAIFFESIN
                     KVYDLATTASRAPSSLWVLVIAAATLLTKIYLLAFTRVNHSKTGNPTLKALANDQLND
                     IMASGAVIVGVVAARLGLPWMDPAAGAIVALFIFKTGVSILMESSAELMDAVPDKDFG
                     DTVRKIALSVEGVRCIEDMGVHRFGPFFTIEMTICVDGSITVEEGNDIAHRVEEKLLE
                     SYEGGLRRVMIHFHPETQHSHFE"
     gene            complement(6988..>7183)
                     /locus_tag="PLB85_01940"
     CDS             complement(6988..>7183)
                     /locus_tag="PLB85_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72117.1"
                     /translation="VDRHQVPDPAEWEKDKKKLLSEAKDKARQEHLNEWYRDERQKVN
                     LIDNRRDFYDLNTPGRVIQL"
BASE COUNT         1677 a         1999 c         1923 g         1584 t
ORIGIN      
        1 tttgaggatg gcgtaccagg aaacattata ggcgggatag ttatccccga ggccataatc
       61 gcttgtccag ctataacctg gctgcatata ctgggaatat tccgcctggg tgacctcata
      121 tttgccaatg tagaaagagt tcagggtgac catatgggtt ggcagttcat ccgaatatcc
      181 gctgccagtg gttctaccca tggtgaaggt gccgccgggg acataaatca tctgaacggg
      241 ggggggtgga ggcgcactga tggtataggt ctcgctggca gtagcgcttg gcgtccagcc
      301 attcttgaaa cccttggcct tgatggtggt tgtagaagac acattgatcg gattgctgta
      361 ggtgggagag ctttccgtcg gctccgcacc gttggtagtg taacggatca cagcaccgct
      421 tgtcgaacaa ctgatcagca cactttgggc tgatgtgtaa gagcctccgg gaggactgaa
      481 agtgggcgtg gcaactgttt gaatcggcga gattgtatat tccgctgtag ttatagagct
      541 gtcagtccag ccagatttgg tcgcttttgc tttcagagta gcgttctggg acacggtaat
      601 ggggctcgag taaaccgggg aggatgaatt cggatctgct ccgttggttg tgtaccttat
      661 ggtcgcgccc gtggtcgctg tgcttatgac aacttcaacc gcgctatcat aagtgcctcc
      721 gggaggactg aagaccggct tggacacctg ctctaactcc gtcggtttgt ggcagccaga
      781 caagaataaa agaaaaacaa cggctgtcaa catgactaca agaagaaaac gcgctttcat
      841 gttacactcc tttggtatta tcttactgac caaggctaaa agccataaat ggcaatctcg
      901 ctgttgaaac ggttgttaaa atgatatcca ttctctcagt aggacggatt gtatcaaagc
      961 cattcatcgg cagaggccaa atttcttaat tgatactggg aaagattgga ttcctcagta
     1021 acttaaaggt atctgaatat tccacgccag tgattccacg cagcccaact aacagcaaaa
     1081 caaaaatcca gtcgctaaaa ctataccttt cataccaagc tcccttttac tggagttatc
     1141 gccagtttgg caagccaaac ccatatccat caatggctca gcaatatgtt ccttttaaga
     1201 cttgccctaa aattcactta ttcgccaata atataatatg tcaagaagat ttttcggttg
     1261 tggcaaaatc cgagcgccat ttcccgtcat tccggcactg gggcaggttt ccagtcccat
     1321 tggtgcttag ccctctgaga ccccctcccc atacgcctcc cggaggatgg ccgcatttga
     1381 tacggggatt gtgcgggagg cgaactacct gcgttgctta aagggctaag gtggcatccg
     1441 gtttccgcag gaaaaatcct ttccgggttg gcgggcagga tttttattga cttcatggct
     1501 tggcgagaaa tcaatgctcc gaataaaggt gaacgatgaa aaagctgtat gttataatga
     1561 ttctggccct tgccgccgtc ggcctctgcg cccaggaagg gctgtttggc cttgcctacg
     1621 gcgacgatct gaacttcgcg gattcgctga tggtgcagca gggcttcatc gcccgcgagg
     1681 tgatcagcag ttgggtgatt tatacctccg attacaatcc cctgatcgat ttcgtggtgc
     1741 tctacgttga tcccgccacg gaaatcctga ggggatggag cgttgtttac aacgccgaaa
     1801 acactcagga acaggatggc tacgtgctcg acaggctgca taagatgcac ggggagaacg
     1861 tgttggtgaa tgaggccagg gagacggtaa gctggatttt cgacgaaaac ctggccgcca
     1921 gctattccta ttcaccgcag gggaaccttc gcgttatgta ttacgatttt tcctgcccgg
     1981 aactgttcgc tctgcccggc accgagaaag agccagagcc ctctgaaccg gcggaaccgg
     2041 atgtggagga aaaaaaccag gcccgctgaa ggggctccaa ttcatccagg caggcttagg
     2101 gcatcccata gtgcttgaca aatcctgcca gcctgaaaag ctgacttact cgataaagaa
     2161 taattaacaa aatagatatc agtatcttag aggagttgaa tatgaaaacc ggcggctgcc
     2221 gtttcggcac tcacagggtt atcgaaccca agggcgtact gccccaacct gccagagttt
     2281 tgaacaatga catgagcgag atctgggata atgaactgct catcgacgtg atccgcctca
     2341 atgtggattc cgcctctttc caccagatca agaacaaact tatcgcccag ggacacacag
     2401 acctggaaaa agccttcgcg gaccacgcta tcgatttggt taaccgcacg ggcaagcaca
     2461 aaaacgagga cacaggctcc ggcggaatgc tgatcggccg ggtggcgcag atcggcccca
     2521 agtttgagat gaaagacgaa atcgccgtag gcgacaccat cgccagcatg gtctcgcttt
     2581 cgctgacgcc gctgaaaatc aacaaggtca agcgggtgct gctggacaag gaccaggtgg
     2641 aaatcgaagg ccaggcaatc ctcttcagca gcggcatcta tgccaaactg ccggacgaca
     2701 tggacgaaaa cctggccctg tcggttctgg acgttgcggg cgctccagcc caagtggaac
     2761 gtttggccag gcccggcgac acggtggttg tcctgggcgc caacggcaag agcggaatac
     2821 tctgcaacgc cattgcccgc gagcgggttg gcgtttccgg aaaagtgatc ggcatcgtgc
     2881 gcaatccagc ctacatcgaa acctgcaaaa acaccggctg ccacgaggtg atcctcgcca
     2941 gcgccaccga cgccatcacc atccagaaag aagtggcgcg cctcaccaat ggccggatgg
     3001 ccgatgtggt gataaacgtg gtgaacatcg aaggcacgga actgccttcc atcatggctt
     3061 gcagggaacg tggcctggtc tatttctttt ccatggccac atctttcacc aaagccgccc
     3121 tcggcgctga aggcatcggc gctgacgtgg atatgatcct cggcaacggt tatgcgcgcc
     3181 accatgccgc catctccctc gatctgctgc gccggaaccc tgtgctgatg aagcttttcc
     3241 aggaaagata caccgacaag tgaggtgctg aatggcagaa ctgacagaca tccgcagcat
     3301 tgccaccgaa caggaatgga acgactggcg ctggcaaatc cgcaaccgca tcaccgattt
     3361 tgagaccctt aaaaaataca tagagcttca gccggaggaa gaggccgtcg ccagctccga
     3421 tagtttttct ttccgcatgg cgatcacgcc ttattacctt agcctgatcg atcacaaaaa
     3481 ccccaacgat cctgtgcgcc tgcaagccat accccgcatc gcggaaagcg ttaccggtcc
     3541 ttccgatatg gccgatcccc tgaacgagga tgccgacgcg cctgtgccag gcatgaccca
     3601 ccgctatccg gaccgcgtgc tgctgctgct cacggaccag tgctcaatgt actgccgcca
     3661 ctgcaccaga cgccgcaaag ccggggagta tgacgccccc atgccgaagg aaaatgtgga
     3721 caaggccatc aagtacattg aagagcacaa ggaagttcgc gacgttatcc tctccggagg
     3781 cgatcccctc accatgagcg acgcccgtat cgatgaggtt ttggttcgtc tggccaaaat
     3841 cgaccacgtt gatatcgtcc gaatcggcac cagaacccct gtggtgatgc cgcagaggat
     3901 caccgacgaa ctgataacca tgctgaaaaa atacaagttc gtctggctga acacccattt
     3961 caaccatccc catgagataa ccccggattc cgctgccgcc ctggccaaac tggcggaagc
     4021 gggtgtgttg cttggtaacc aatccgtgtt gctaaaaggc gtgaacgacc acgtggatgt
     4081 gatgaaatcc ctggtgcacg ccctggtgcg caaccgcgtg aggccttatt acatctatca
     4141 gtgcgacctt tctgaaggca tttcccattt ccgcaccccg atttccaaag gcatcgaaat
     4201 cattgaatcc ctgcgcgggc acacctccgg actttgcatt cccactttcg ttgtggatgc
     4261 ccccggcggc ggcggaaaga tccccgtgat gcccaattat attatctccc agatgccggg
     4321 acgcgttgtg ctgcgcaact atgaaggctt catctccgca tacacggaac cggaattcgc
     4381 gcccctggat gaaagcaaat accagtcccg ctgcgcgcct gaacgccagt caacagaggg
     4441 cgtgatgacc ctgctgcggg gcaaaaaggt gtccataggg ccggcagaca cccgccgcaa
     4501 ccaacgccgt accaaataac cggcgccaaa agatgaaaca cggagcgtgg cccggaccct
     4561 ttgactggat ccaggccgcg gaaccgcgcg ctgtagccat cgtcggcatt tgcaagaacg
     4621 ccgggaagac caccctgctc aatcaccttc tatccctgcg caaaaaagga gtgtggggtg
     4681 ttttcagcac cgggatcgac ggagaggaac gcgacagcgt tttccgggtg cccaaacccg
     4741 cggtcaaact gggaccggac atcatattct gttgcgacac tgccaatctg gacgctttgg
     4801 gcagcggtgt caaagtgctg gaaaaggtcc ggggcagttc tgcggcccgt cctttgtggc
     4861 tggcgcgcag cctggcgccc ctgcaaactg agatcaccgg ccccggttca gtatccgagc
     4921 aaatctccgt gctgcagacc atgtgggaac agggagcggg aaaagtgctg atcgacggtt
     4981 cgctggaccg caaatcgata gccctgtctg aagctgtcga tgccgtcgcc gtggtgatcg
     5041 gcgccagctt tggcagtgtg tcttccgtca tcactgaaat caggcgtctg gagcttctca
     5101 attctcttcc tgaggcgaag ctcggacaac aggaatttgc aaagctgctc ggttcggaga
     5161 aaatccttct gaaaaaagac ggatgctgga ctggaagcga cctgcattcc ctgctgggaa
     5221 atgagaagga actgaaaaag ctgttggaac agggtcatga agccatttac atccccgggg
     5281 cgttgactga tgcagtattc ccggctttgc gggaaccttt ggcagcgctg aagacaaaca
     5341 tcattttccg ccatccggag tgcctgaaac tggggctgac acgcttggaa cgcttggttg
     5401 aggagcttca accgcaggtg ctgattccct tccgaatctg gtcctggaca gtgaacacgg
     5461 cttccgttgg caatgtggct gtcgatgcag cggactttcg tgcccggctg cgagaggctt
     5521 ttccgcagct tgacctgcct gatataaggg agctaacgtc atgagcgaca gagacaagat
     5581 caccaaccgc actgtcaatt ttggcctgtt cacaaacgtg gtcctggctg tgctgaaatc
     5641 cctcatcggt attattggcc attcgcaggc cctcttggct gatggcataa actctacatc
     5701 agatgtggtt tactacgtgg cggtgaagat cttcatgcgt caggccaaca aacctgccga
     5761 tcctgaacat ccctacgggc accgccagtt ggaaagcatt tccgccatcg tggtgggcgc
     5821 gttcatcatc accaccggca ttgccatctt ctttgaatcc atcaacaagg tttacgacct
     5881 ggccaccacc gcttccagag cgccaagctc actgtgggtg ctggtgatag ccgctgccac
     5941 cctgctgacc aagatctatc tgctcgcctt cacccgcgta aaccacagca agacaggcaa
     6001 ccccaccctc aaagccctgg ccaacgacca gctgaacgac atcatggctt ccggggccgt
     6061 gatcgttggc gtggtcgccg cccgtctggg actgccctgg atggacccgg cagccggtgc
     6121 catcgtcgcg ctgttcatct tcaaaaccgg ggtgagcatc ctcatggaat cgtcggctga
     6181 actgatggac gccgttccgg acaaggattt cggcgatacc gtcagaaaga tagcgctttc
     6241 cgtggagggc gtgcgctgca tcgaagatat gggcgtgcac cgttttggcc cctttttcac
     6301 catcgaaatg accatctgtg tggatggctc catcaccgtg gaagaaggca atgacattgc
     6361 ccaccgggta gaggaaaaac tgctggagag ctatgaaggc ggcctgcgcc gggtgatgat
     6421 ccatttccat ccggaaacgc aacactctca ctttgagtga gatacctctc tttcccatgt
     6481 ccgcgagctt ttcccgctga cccgtcctaa gaatccaccc gcataaagga actctctttc
     6541 aagccaggcg gataaagacc aacagacagc tttttgtttg agcgtccgca ccgacttgtt
     6601 tgataagaga gtcgtatctg tttacccacc gcaatatcct gccagttcgg tcctgaacgc
     6661 ttgagtccac cgctctctca cgccccgcgc atgattctcg catcaaatgc gggtatcgtg
     6721 cgggaggcgt gtctggaaca caaacaaggg ccttaacggc ctgacggcat cgcttctccc
     6781 aagtcactta cccccccgct attgtattca gccccaaagc cttacccatc cgatttgaaa
     6841 gagggacaaa ctcatcttgc cgtcgctcat gttgacgctt ccggaaatcc gatccatgaa
     6901 aaagggacca agagccattt ctggccgttg gtcccgataa tgtcttcagc cgaccgcccc
     6961 aattggctgg ggcgaagcgt tcgagctcta gagctggatc accctgccgg gggtattgag
     7021 gtcataaaag tcacgccggt tgtcgattaa gtttactttc tggcgttcat cgcggtacca
     7081 ttcgttcagg tgttcctgcc gggccttgtc tttggcttct gaaagcagtt tctttttgtc
     7141 cttttcccat tcggccggat ctggtacctg atggcggtca acc
//
LOCUS       DAOOWR010000042         7177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10453_length_7177_cov_4.167369, whole genome shotgun sequence.
ACCESSION   DAOOWR010000042 DAOOWR010000000
VERSION     DAOOWR010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10453_length_7177_cov_4.167369"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="PLB85_01945"
     CDS             complement(<1..1824)
                     /locus_tag="PLB85_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72118.1"
                     /translation="MPYLGVPTANPASYPTQIMIGNTSYAITGAAPYTRVGLTMNGVI
                     YGSGITDASGNLTLTISPFTSVGTAKLVLTAQNKITKIADIAVIAAQGPFMSVEQVAY
                     DDANNDIPEYNESGYFDVTFKNVGVDPASNVSAILSCSTSGITITDNSHSISSLAAGA
                     TVVANNAYAITIANNVADGTVANFTITMTMSGHNPWTHNFTLTLNAPALTFGNMTISD
                     PTGNNNGRLDPGETVTITMPLNNNGGAASPNGTATMSCATPGITVNNGNASFSAISAG
                     GVSNLTFSLTANASMDIGTVVSLNFAATAGQYSASKTENTPVGLIIEDFSTGDFSSFP
                     WIMGGTLPWTIDNSTYYSAGYSARSGAISHSQTSTMETTRVLTAPGELSFWYKVSSES
                     NYDKLRFYVDGAQQNEWSGNIDWTQATYNLAAGTRVLKWEYVKDYSVSSGSDCAWVDD
                     IIFPASTTPSSFYPPRNLTAAPGNGYVTLNWQAPLTGTPTGYRIYRDGNLLTTVTGLT
                     YTDNAVVNETTYSYYLVAVYSGGVSDPTDTVQATPSAIVPTEVIIGTGTDSNSTSIAC
                     PINVWYQSLHGQSVYTAAELNAAGVVGPINITQIGFNVTGLP"
     gene            complement(1882..3647)
                     /locus_tag="PLB85_01950"
                     /pseudo
     CDS             complement(1882..3647)
                     /locus_tag="PLB85_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
     gene            4088..4435
                     /locus_tag="PLB85_01955"
     CDS             4088..4435
                     /locus_tag="PLB85_01955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU72119.1"
                     /translation="MLHSSLSGNALPRFYLMLDGERVAGCCALLTNDLISRQDLWPWL
                     ACLYVEPDYRGRALGATLLEHGAREAKRMGYGALYLTTDHDGYYEKYGWSRMEDGYNL
                     FGERSRIYWLELN"
     gene            complement(4439..5833)
                     /locus_tag="PLB85_01960"
     CDS             complement(4439..5833)
                     /locus_tag="PLB85_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU72120.1"
                     /translation="MRRFVTVLLALAAVAGLAATDISGSWNGMLDAGGQKIRVVFHIT
                     TTETGLTATMDSPDQGAFGLPVARTEFKDPKLELVMDVPSITYSGELKEGMIAGTFKQ
                     AGMEFPLNLQREALEKPVYIRPQEPKEPFGYRIEEVLFSNPEVGIELAGTLTLPQGEG
                     VFPVVVMISGSGPQNRDEELMGHKPFWVIADHLTRNGIAVLRYDDRGVGGSGGEASTG
                     TTYDFATDALAAVNCLKSRPEFSFIGLVGHSEGGIIAPIVASQSDEVDFIVMLAGTGI
                     RGDKLLQAQGELIAQASGMEKEEVYRTMEVNAGAFRLALQAQDLASFETELRAYLKEK
                     MDDGTIEIPEGITYDELFRMQMDSVANPWMYEFIRLDPAQWLKKVTCPVLALNGSKDL
                     QVPAKQNFPAIGEALEEAGNKDYTLREYPGLNHLFQECETGHPDEYARIEQTFAPVVL
                     DEMTAWIKARTIQK"
     gene            5979..6260
                     /locus_tag="PLB85_01965"
     CDS             5979..6260
                     /locus_tag="PLB85_01965"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOU72121.1"
                     /translation="MSDSFYKAIADRTRREVLSLLRRNGPMSVNEIASHFTISLPALS
                     EHLKVLRNADLVSTRKEKQFVFYSLNTTVLEDLGSWVLDLIRKKEVNDE"
     gene            6253..6912
                     /locus_tag="PLB85_01970"
     CDS             6253..6912
                     /locus_tag="PLB85_01970"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOU72122.1"
                     /translation="MNRLKKYKWALLVLVLHLAAVLWFATQLPEGAKVPIHWNYLNQI
                     DGWTGRTTGLFWGIGLNVLMFLMLYLLHWYSPWYNKYAGRFEKILPPLTTTLLACFST
                     ISLYSLYVAKWGEVPGVKLILVLIGLFLFFFGNLMPKVPKNMFVGIRTPWTIANELVW
                     ERTHRLGGWLFVTGGIVMILKGLILTGSALFQNITTVLALGCLLYPLPYSFIQYKKLV
                     K"
BASE COUNT         1453 a         1859 c         2152 g         1713 t
ORIGIN      
        1 aggcaggcct gttacattga aaccgatctg ggtgatattg atggggccga ccactcctgc
       61 ggcattgagt tcagcggctg tataaactga ctgtccgtgc aggctctgat accagacgtt
      121 gatggggcag gcgatgctgg tgctgttgga atcagtgccg gtgccaatga tgacttcagt
      181 gggcacgatg gcagagggtg tggcctgcac agtgtcggtg gggtcggaca ctccgccaga
      241 atataccgct acaaggtagt agctgtaggt ggtttcgttg accaccgcgt tgtcggtata
      301 agtgagcccg gtgacagtag tcagcaggtt gccgtcgcga tagatccggt agcctgtggg
      361 agtgccggtg agcggggcct gccaattgag ggttacatag ccgttaccgg gcgcggcggt
      421 caggttcctc ggcggataga agctgctggg agtggtggag gcggggaaga tgatgtcgtc
      481 cacccaggcg cagtctgaac cgctgctcac gctgtagtct ttgacatatt cccatttcag
      541 cacacgggtg ccggcggcca gattgtaggt ggcctgagtc cagtctatgt ttccagacca
      601 ctcgttttgc tgggcgccgt ccacatagaa gcgcagtttg tcataattgg attcggagga
      661 gaccttgtac cagaagctca gttcacccgg ggctgtcagg acgcgggtgg tctccatggt
      721 gctggtctgg ctgtgggaaa tggcgccgct cctggcgcta tagccagcgc tgtaataggt
      781 gctgttgtcg atggtccagg gtaatgtccc gcccatgatc caggggaaag agctgaaatc
      841 tccggtggag aaatcctcaa tgatgaggcc cacgggggtg ttttcggttt tgctggcgct
      901 gtattggccg gcggtggctg cgaagttgag gctaaccact gtgccgatgt ccatgctggc
      961 gttggcggtg agtgagaatg tgaggttgct aacgcctcca gcggaaatag cactgaagct
     1021 ggcgttgccg ttgttgaccg ttatgcctgg cgtggcgcag ctcatggtgg ctgtcccatt
     1081 cggggaggcg gcgccgccgt tgttgttaag cggcatggtg atggtcacgg tttcaccagg
     1141 gtcgaggcgt ccgttgttgt taccggtggg atcgctgatt gtcatgttgc cgaaggtcaa
     1201 agccggagcg ttcaaggtca gggtgaagtt gtgggtccag gggttgtggc cgctcatggt
     1261 catggtgatg gtgaaattcg ccacggtgcc gtcggcgacg ttgttggcta tcgtgatcgc
     1321 gtaggcattg ttggccacaa cagtagcccc ggctgccagg ctgctgatgc tgtggctgtt
     1381 atcggtgatg gtgatgccgc tggtggagca gcttagtatc gcgctcacgt tgctggcggg
     1441 atccacgccc acgtttttga aagtgacatc gaaatagccg ctttcgttgt attccggaat
     1501 gtcattattg gcgtcgtcat aagcgacttg ctccaccgac atgaaggggc cttgagccgc
     1561 tatgaccgct atatcggcaa tcttggtgat cttattctga gcggtaagca ccagtttggc
     1621 ggttccgact gaggtgaagg gggaaatcgt cagggtcagg tttccggagg catcggtgat
     1681 gccggagccg tagatcactc cattcatggt aaggccgacg cgggtatagg gcgcggcacc
     1741 ggtgatggca tagctggtgt tgccgatcat gatctgggtg ggatagctgg ccggattggc
     1801 ggtgggaaca ccaaggtagg gcatcaggga gggatcgccc atgatgtggt agacttccca
     1861 gtagtagttt gtgcgtgagc taccgctttg gctaactgcc aggttgccgg agtagatgct
     1921 ctcgccgacg gaggtggccc atttgtcata ggtttcgccg tgagtgtgga acatggcgtc
     1981 ataagcgcca agtttggtgg catcataagg cggggcggaa ccgtcgatgt tggttttgta
     2041 acccactgcc cagtaatagt cctcattcca ataggtgctg ttggtgccgc cgatgtaggc
     2101 cactccgccg ccgtcggcct tgcggatgac ggcttcgccg aagcaggggc tggagtagtc
     2161 aaactcgttg gtgatgcagc agttgcccac cattactcca tatttcccgg agttggtcat
     2221 cgcattgacg tcggagacgg tgaagctggg gtcgtgccag gaggtctggc tcccgtgagc
     2281 ggtatagaca atgtaacccc ggccttcatt ggcgttggcc cttatctggg cggcactgct
     2341 gccggaggcg ggatagaggt aggtgtcgga ggtgatgccg tgggcggcgt tgaaatactc
     2401 agtggttgca taattgatgg cgccgttggc atgagtggga gaccagctgg aatccactcc
     2461 ggcgatgagc actgtcttac ccagataact gatgtccggc atctgggtct tttcaaaagt
     2521 aatggtcttg ctaacgatgt ttgccagttc ggtggtgctg gaaactgaga agcggccgtg
     2581 gtacatttcc tgtagtcagt gccattgagg cgcacatagt tgaggtcggt tacgtgggag
     2641 ccagtcgcgc cggtattggt ggcgactgtt atggtgccgg attcgtcacc gatgatcagc
     2701 aggtaggtgg gggccggatc atcggcagtg gcagcatccc acaaagactg catatagttc
     2761 ttgatggcgg tggtggtgtt tgccacggtg ccgccgctgc cgacggtcgc cacgttcacc
     2821 acaaagccct gctgggtctt ccagtccacg taactctgga tattgctggt gtagccaacc
     2881 gggcagagga tgaccatttt ggtgggatgg cgcaccagag cggtgcggtt gtccgcgttc
     2941 cagttgaaga tagtgttgct gtaaagctgt tcaaactccc aggaagcagt cctggccagc
     3001 atgtcggcgg tggcgatgag gtccgggttg acaaaatcca cccggacatc cagtttttcc
     3061 atcacccgga tactgttggt gacggggttg tagcgcaccg gataaaaatc gagggcgaaa
     3121 acccgcacac cgcgcatcat gccaagttcc gaaacctgca cccagtcgcg gctgttgaac
     3181 tcatcgcggc tgtaaagcat ttcgttcacc acaaaaggaa tggtggcagg atcggcggat
     3241 ttggagatgg gctcctgggc cggaatgacg cggttttgca gtttgttggc gttgttgtca
     3301 aattcccgag cggattgtga attgagggtt aggcggactt ccgcgcccaa aggcacagcg
     3361 atggtcctgc gcagcatggg cagcttgggc tcgcccactt cgttgctgaa gccccaccct
     3421 tcgatggaaa gctcgtcaaa cgtgccttcc ttggtctgga cttcaatgat cttcagttcg
     3481 ccaacgctgt agttgacgtc aaaaccgagg tcgctgttgt ttctaagcag agcttggttt
     3541 gtagtggggc tcagctccac ctgccgggtc tgggcagaca gggccaaggc gaaggtcagg
     3601 gtgagcgcta gcagcagcaa caccttacct ttgaacttgt tgtgcatacg atactcctat
     3661 atgcttgatt gcttctgact tgaaattctt gcacggtggc agatggcaaa ccaatccgcc
     3721 aagcttatac attcgcgcaa tcaattccag atgcgatatt tgtcaactga taaatgagag
     3781 acacgtaaca ttgtgcaagt aatgcggatc aggcgcactt atgaatattc atcaaccgga
     3841 tttcagcgtt tcctgcattg agccgggcag ttttccagag aaatgacttg actgatctgg
     3901 cagtgtggat gtaatctcaa cctaacaaac ttactgcctg tctggataag tatgacaaaa
     3961 caatgccaag caaggaaatg atggaaataa tcaacgttcg tgatcacaga tcgggcctcg
     4021 aatccgctgc cgctacatcc acggcatctg gggccggcct caaaacctgg ccttctacct
     4081 cgatgccgtg ctacactcct ccctttcggg aaacgcgttg ccccgcttct atctaatgct
     4141 cgatggagag cgggtggcag gctgttgcgc gctgctcacc aacgatctga tcagccgaca
     4201 ggacctctgg ccctggctgg cctgcctcta tgtggaacct gattaccggg ggcgggcttt
     4261 gggcgccacg ctattggaac atggcgcaag ggaggcaaaa agaatgggtt acggcgccct
     4321 atacctaacc accgaccatg atggctatta tgaaaagtat ggctggtcca ggatggagga
     4381 tggttacaac ctcttcgggg aacgcagccg catctattgg cttgaactga attgaaggct
     4441 atttctgaat cgttctggcc ttgatccagg cggtcatttc atccagcacc accggagcaa
     4501 aagtctgctc tatcctggca tattcgtccg gatggccggt ttcgcattcc tggaaaaggt
     4561 ggttcaggcc tgggtattcg cgcagggtgt agtctttgtt gccggcttct tccagcgctt
     4621 cgccgatggc ggggaaattt tgtttggccg gcacctgaag gtctttgctg ccattcaaag
     4681 cgagcaccgg acaggtgact tttttcagcc actgggcagg gtcaagcctg atgaattcat
     4741 acatccaggg attggccacg ctgtccatct gcatcctgaa tagttcatcg taagtgatgc
     4801 cttccgggat ttcgatcgtg ccgtcatcca tcttttcctt caggtaggcg cgaagctccg
     4861 tttcaaagct tgccaggtcc tgagcctgca gggccagcct gaaggccccg gcgttcacct
     4921 ccatggtccg gtagacctcc tctttctcca tccctgatgc ttgggcgatc aattctccct
     4981 gcgcctgcaa aagcttatcg cctcggatgc ctgttccagc caacatcacg atgaaatcca
     5041 cctcgtcaga ttgagaagcg acgatggggg cgatgatgcc accctcgctg tggcccacga
     5101 gcccaatgaa actgaattct ggacgggact tcagacaatt aaccgcggcc agggcgtcgg
     5161 tggcaaaatc gtaagtggtt ccggtggaag cttctccgcc ggaaccgcca actccgcggt
     5221 cgtcgtatct caacacagcg attccgttgc gggtaaggtg gtcggcaatc acccaaaacg
     5281 gcttgtgccc catcagttct tcatcccggt tctggggacc gctgcccgag atcatcacca
     5341 ccacagggaa aacgccttcc ccctgtggca gggtgagcgt tccggccagt tcgatcccca
     5401 cttcgggatt ggaaaagagc acttcctcga tcctgtatcc gaaaggctcc ttgggttcct
     5461 gcggcctgat gtaaacaggt ttttccagcg cttcccgctg caggttcaga ggaaactcca
     5521 tcccggcctg cttgaaagtc cccgcgatca ttccttcctt cagttcgccg gagtaggtga
     5581 tcgaaggcac atccatcacc agttccagct tggggtcttt gaattcggtc ctggccacgg
     5641 gcaagccgaa agcgccctgg tccggactgt ccatggttgc ggtcagccca gtctccgtgg
     5701 tggtgatgtg aaaaacgacg cggattttct gcccgcctgc gtccagcatc ccgttccagc
     5761 taccgctgat atccgtggcg gcaaggcctg ccactgccgc aagtgccagc aacacagtga
     5821 caaatcttct catcatatac tcctcatgtt gtgatcccca tctgtatcct gtttgataca
     5881 ttcccggcat ctgtcaagca aaaacgcaga aagttcttga cttatttcgg actttgccta
     5941 attaggcatt cagttaaaca ccaaacaaag gagaaacgat gtcagattca ttctacaagg
     6001 ccatcgcgga ccgcactcgg cgggaagtcc tttccctgct gcggcgcaac gggccgatgt
     6061 ccgtgaacga gatagcaagc catttcacta tcagcctgcc agccctcagc gagcatctga
     6121 aggtgctgcg caacgcggac ctcgtttcca cacgcaagga aaagcaattc gtcttttaca
     6181 gcctcaacac cacggtgctt gaggatttgg gcagttgggt gctggatctg atcagaaaaa
     6241 aagaggtgaa tgatgaatag actgaagaag tacaaatggg cgctgctggt cctggtgctg
     6301 cacctggcag ccgtgctctg gttcgccacc cagttgccgg aaggggcaaa ggttcccatc
     6361 cactggaact atctaaacca gatcgatggc tggacggggc gcacgacagg cctcttttgg
     6421 ggcattggat tgaacgtgct gatgtttctg atgctctatc tgctgcactg gtattccccc
     6481 tggtacaata aatacgccgg gcggttcgaa aagatcctgc ccccgctcac caccactctc
     6541 ctggcctgtt tctccaccat cagcctctac tcgctgtatg ttgccaaatg gggcgaggtg
     6601 cccggcgtga agctgatcct ggtcctcatc ggcttgttcc tcttcttttt tggcaatctg
     6661 atgccgaaag tgcccaaaaa catgttcgtg ggcatccgca cgccctggac catcgccaat
     6721 gaactggtct gggaaaggac ccaccgcctg ggaggatggc tgttcgtgac aggcggaatc
     6781 gtcatgatcc tcaaaggcct catcctgaca ggctccgcgc tgttccagaa cattaccacg
     6841 gtgctggctt tgggctgcct gctctatccg ctgccgtatt cgttcataca gtataagaaa
     6901 ctggtgaagt gagaaggact taggctcatt tttcaaatat agtgggtggg actgccttca
     6961 ttccagcact ggataccccg cgaaggattg aagcgacaag gtctggccgt taaggccctg
     7021 tcctgtggcc ctgatccgcc tcccgcacga tgcctgcatt tgatgcgggg attggacggg
     7081 aggcatggga gtgggatgga ctcgggcgct aaggacctgg ctgcaggata ttgctatgga
     7141 taagctggtt atggctcttc attaaatcaa gagggtg
//
LOCUS       DAOOWR010000043         7145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10513_length_7145_cov_4.264034, whole genome shotgun sequence.
ACCESSION   DAOOWR010000043 DAOOWR010000000
VERSION     DAOOWR010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_7145_cov_4.264034"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLB85_01975"
     CDS             complement(<1..778)
                     /locus_tag="PLB85_01975"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOU72123.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGVTLEARKFQQVTRVYGALY
                     GVQGIVNEQFIDLTQETTHNLEQVANTPSSALLSTRDKPDEQYCREIFRVLKAHGVRY
                     FFYIGGNDSADTVRIVNEEADQADYEFRAIHIPKTIDNDLVLSDHTPGYGSAARFVAS
                     AFTGVNLDNRALPGVYIGVVMGRHAGFLTAAAALAQKYPDDGPHLIYMPERPFRQENF
                     LDDVKRVYDQYGRCVVAVSEGIVDEHGTPVMTILTKNSETD"
     gene            922..2499
                     /gene="dnaX"
                     /locus_tag="PLB85_01980"
     CDS             922..2499
                     /gene="dnaX"
                     /locus_tag="PLB85_01980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU72124.1"
                     /translation="MSYLVLARKYRPQTFKEVYAQDHITEILNNALLSNRIAHAYLFT
                     GPRGVGKTSMARIMAKNLNCVEGPTITPCNKCHNCLEIAAGTSADVIEIDGASNTGVD
                     DIRELQRELLYAPSQSPWKIYIIDEVHMLSKNAFNALLKTLEEPPEKVLFIFATTEPF
                     KVLPTIISRCQRYDFKRIPIDAIVKRLKELMLEEKIEIDSESLHLIARKADGSLRDAL
                     SLTDQVLSYCQDKVTIDKVREIFGLIPTQIYCELMRLLHARENSALLEKLQAIFEEGV
                     DLQEFLNNMMEFIRIVILRKIGMSPSEVTSEEYPLYDGIVALFSRENLLYVMSMLLQA
                     RNEIKSSGNPYLIFELMMLKLCKLDEMEDVAKLIANLSSGAVSAAPARGSAPAAVQKP
                     EPIRTAEPVPSNKLDFSQENLDKVWERIKARVKKESGASGIALNFSSCEVKDRFKLLI
                     KVTGSTYFKTLDGYKDEITKIVAEFFSQAPRLELEQLASEAPTKVEIKRHTLEDLKNL
                     DKNLARFIEITDSKISQ"
     gene            complement(2632..2880)
                     /locus_tag="PLB85_01985"
     CDS             complement(2632..2880)
                     /locus_tag="PLB85_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72125.1"
                     /translation="MKYKLILIKIDRRSTEAASVQSILTDYGCNIKVRLGLHEVSKEF
                     CANDGLIILEVEGDKRKLNTIVKKLNALEYVTAQLIEM"
     gene            3019..3423
                     /locus_tag="PLB85_01990"
     CDS             3019..3423
                     /locus_tag="PLB85_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72126.1"
                     /translation="MKYIGSILAMLGGLIVILAALVSLVSKEVRGIWTYEFSSTQEFF
                     VVAAILAIMITILAFLSFSTKPKLLGSIIIFASFAGIIVGSTLTDIAMIFSILGGLAL
                     YTMPKQEKEADMEEPSCLKPEQPDEPATPNPS"
     gene            complement(3439..4026)
                     /locus_tag="PLB85_01995"
     CDS             complement(3439..4026)
                     /locus_tag="PLB85_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72127.1"
                     /translation="MKKLAIATLIAILALAVMPAWAEEGWKTLNSTEHRFSLDYPDDW
                     DHEVSIDALLTLMKTDGGLPMVITVAAEKDTSNAENGIMPKYLETEMDAVEREIAALG
                     MGDAVTLERGESQVNGLPAYVLDLKLDLMGMAQIRMYNVIVDMGSSLVYLSFSGENGS
                     YMKNAETFTKIKNSLRPIGEASELQPAPESREREY"
     gene            complement(4121..5806)
                     /locus_tag="PLB85_02000"
     CDS             complement(4121..5806)
                     /locus_tag="PLB85_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU72128.1"
                     /translation="MEISEKTVISNFIRTIIDKDLASSKHSKVVTRFPPEPNGYLHIG
                     HAKSICLNFGLARDYGGVCHLRFDDTNPIKEDVEYVDSIMADVRWLGFDWQDNLFYAS
                     DYFDQLYEYAEILIQKGLAYVCDLSQEEIRAHRGTLTVPGDDSPYRERSVEENLDLFR
                     RMRAGEFEEGTKSLRAKIDMASPNLNMRDPVIYRIKKAEHHRTGIKWVIYPMYDYTHC
                     ISDAIEGITHSICTLEFEDHRPLYDWFLDQLPVPCHPQQIEFARLNLSYTVMSKRRLL
                     ELVESGAVSGWDDPRMPTIAGMRRRGFSPEAIREFADRIGVSKATSIVDFELLQFCVR
                     EDLNKRANRVMVVLEPLKLTIENYPDGKFEELEAENNPEDPGAGKRLVPFGKHLYIER
                     GDFCENPVKGWFRLSPGAEVHLKHAYYVTCREVVKDTEGRVTEIVCSHDPESRGGWTS
                     DERKVKGTIHWVSAAHAIDAEVRLYDQLFTIADFNAMEEGKDYRDYLNPASLQVLTGC
                     KLEPSLAKAVQGERFQFLRQGYFCADDDYTQEKPVFNRIVTLKDSWAKRSKKR"
     gene            5984..7144
                     /locus_tag="PLB85_02005"
     CDS             5984..7144
                     /locus_tag="PLB85_02005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU72129.1"
                     /translation="MKRLLILSDLYPTCQDSQKGIFVKHQVDELAHYYRVLVIAFHIG
                     GSFKVERYIQGQVSACSITYPALKVPFLSAFASFPVFALPVARQVCSRFRPDLIHVHD
                     FRHIPELFWLKTWLDRLKQPKILTLHNIRTHPDRLGGNRLLPFYRKTMPKAFARWDHV
                     FTVNKRLRDWLLPWQDADRISVLGNAIGPAPDCREEELIPIRSGLRKDSYKLLSVGNL
                     TPEKGFSYLIDAVKILTDKGLDLQLVIVGKGTQRASLKAQISALGLDGIVRLTGGLNN
                     HIVRNVYQYFDLFVLPSYSETFGIVFLEAMDAGLPVLGVEGEGIHGLFEQGREALFAQ
                     PRDSISLSNSIARFIQEPELATQLAHAGKMRVNKDFMMPGLIGRVREVYEQQ"
BASE COUNT         1643 a         1960 c         1809 g         1733 t
ORIGIN      
        1 cgtcagtttc gctgttcttg gtgaggatgg tcatcacggg ggtgccgtgt tcgtctacga
       61 ttccttccga aacagctaca acgcagcgtc catactggtc gtaaacgcgt ttcacgtcat
      121 ccaggaaatt ctcctgcctg aaaggccttt cgggcatgta aatcagatga ggcccgtcat
      181 ccggatattt ctgggccaga gccgcggcgg ctgtgagaaa accggcgtgc cgacccatta
      241 ccacgccgat gtaaactccg ggcagggcgc ggttgtcgag gttcacccct gtgaaggcag
      301 aggccacgaa tctggccgcc gagccgtaac ccggggtgtg atcgctgagc acaaggtcgt
      361 tatcgatagt tttgggaatg tggatggcgc ggaattcgta atcggcctga tctgcctctt
      421 cgttcacgat gcgcaccgta tcggcggaat cgtttccgcc aatataaaag aaatagcgga
      481 cgccatgggc tttcagcacc cggaaaatct cgcggcagta ctgctcatcc ggtttgtcgc
      541 gcgtggacag cagcgccgag gagggcgtgt tggccacctg ttccagattg tgagttgttt
      601 cctgggtgag atcgatgaac tgctcgttca cgatgccctg cacgccgtac agcgcgccgt
      661 aaacccgcgt cacctgctga aatttccttg cctcgagggt aacccccacc aaagattggt
      721 tgatcacagc cgtcgggcct ccgccctggg caatcaccac ttttccttcc aatttcatct
      781 tatacctcca ctgcttttca taatatcagg aactcccggc aaccgccagg ttaattcact
      841 ttatataaag ccgccgaatt tgtccagcag aaaatgcttg cctgaaagac cgggctgaaa
      901 aagaatgacg acggagcaca gatgagctat ttagtacttg ccagaaaata ccgtccgcaa
      961 accttcaaag aggtttacgc gcaagaccat atcaccgaaa tactgaacaa cgcgctgctt
     1021 agcaaccgca tcgcccatgc ctaccttttc accgggccgc ggggagtggg taaaacctcc
     1081 atggcccgca tcatggcgaa aaatctcaac tgcgtggaag gccccaccat cactccctgt
     1141 aacaaatgcc acaactgcct ggagatcgcg gctggaacat ctgcagacgt gatcgagatt
     1201 gacggagcct caaacaccgg cgtggacgac atccgcgagc ttcagcgcga gctgctttac
     1261 gcgcccagcc agtcgccttg gaaaatctat atcatcgacg aagtgcacat gctttccaaa
     1321 aacgcgttca acgccctgct taaaacgctg gaggaaccgc cggaaaaagt gctcttcatc
     1381 tttgccacca ctgagccctt caaagttttg cccaccatca tttcccgctg ccaacgctat
     1441 gatttcaaac ggattccgat cgatgccatc gtgaaacggc tcaaggaact catgctggag
     1501 gaaaaaatcg agattgactc ggagtcgctg cacttgatag cccgcaaggc cgacggaagc
     1561 ctgcgcgacg ccctttccct caccgaccag gtgctttcct actgccagga caaagtgacc
     1621 atcgacaagg tgcgcgaaat ctttggcctc atccccaccc aaatctactg tgaactgatg
     1681 cggctgctcc acgcccggga aaactccgcc ctgctggaaa agttgcaggc catctttgag
     1741 gaaggagtgg acctgcagga attcctgaac aacatgatgg aattcatccg catcgttatc
     1801 ctgcgcaaga tcgggatgtc gcccagcgag gtcaccagcg aggaatatcc gctctacgac
     1861 ggcatcgtgg ctctcttcag ccgggaaaac ctgctctatg tgatgagcat gttactgcag
     1921 gcgcgcaacg aaatcaagag cagcggcaac ccttatctga tctttgaact catgatgctc
     1981 aagctgtgca aactggatga aatggaagac gtggcgaagt tgatcgccaa tctatcttcc
     2041 ggtgccgttt ccgccgcccc ggcgcgtggc tctgcccctg ctgcggtaca gaaaccggaa
     2101 ccgatccgga cggcggaacc agttccgagc aacaagctgg attttagcca agaaaacctg
     2161 gacaaggtct gggagcgcat caaagccagg gtcaaaaagg aaagcggcgc ttccggaatc
     2221 gcccttaatt tctccagctg tgaggttaag gatcgtttca agctactgat caaagtcacc
     2281 ggcagcacct atttcaagac tctcgacggt tacaaggacg agataacaaa aattgtagcg
     2341 gaattcttct cccaagcgcc gcgactcgag ctggaacaac tggccagcga agctccgacc
     2401 aaagtggaaa tcaaacgcca taccctcgaa gaccttaaga atctggataa aaacctcgcc
     2461 cgcttcatcg agattacgga ttccaaaatc agccagtaaa gcgccctcct ttttccccat
     2521 ttagcggccg gaaacaagat caggcgccaa gcatggcact aacccgccaa agctgatttg
     2581 aacgggattc caggctggcg ccgaattcag ttttccccgg aagggaaatg actacatctc
     2641 gatcaactgc gcagtgacgt attccaacgc gttgagtttc ttcacgatgg tgttcagttt
     2701 ccgcttgtca ccttcaactt ccaaaattat taggccgtcg ttggcgcaga attccttgga
     2761 aacttcgtgc aagccaaggc gcaccttgat gttgcagccg taatccgtga gaatgctctg
     2821 cacgctggcg gcttctgtgc tacggcggtc gatcttgatc aggatcagtt tatacttcat
     2881 tagttcctcc cgatttcata ttttcccaag ccggtaaatc tgtccacata tttttcgcct
     2941 gctgggcaac cggagaattc tgttgacatt gagagggttt gaagtttgat ggctcatatt
     3001 ttgaaactgg agcaaacaat gaaatacata ggcagtatct tagcaatgct tggaggattg
     3061 atcgttatac tggcagcgct ggtttcgctg gtttccaaag aagtcagggg catctggaca
     3121 tacgaattca gcagcactca ggaattcttc gtcgtagcgg ccatcctggc catcatgatc
     3181 accatcctgg ctttcctttc tttttccacc aaacccaagc tgcttggatc gatcatcatc
     3241 ttcgcttcct tcgcggggat aatcgtggga agcacactta ctgacattgc catgatcttc
     3301 agtatcttag gcggattagc cctgtacacg atgcccaagc aggaaaaaga agcggatatg
     3361 gaagagccgt cctgtctcaa acccgaacag cccgatgaac cggcgacccc caatcccagt
     3421 tgagggtcca ctattaaatc agtattctct ttcccgtgat tcaggcgctg gctggagttc
     3481 tgaggcctca ccgattggcc tgaggctgtt cttgatcttg gtaaaagttt cagcgttttt
     3541 catataggag ccgttctcgc cagaaaagga gaggtaaacc aagctggaac ccatatccac
     3601 gattacattg tacatgcgta tttgcgccat tcccattaga tcaagtttaa ggtccagcac
     3661 ataggcggga agcccgttca cttggctttc accgcgttcg agggttaccg cgtctcccat
     3721 ccccaaggct gcgatctccc tttccacggc atccatctca gtttccaaat atttgggcat
     3781 gatcccgttt tccgcattgg atgtatcctt ttcggcagca acagtgatca ccataggcag
     3841 gcctccatca gttttcatga gtgtcagcag cgcgtcgatg gatacctcgt gatcccaatc
     3901 gtcaggataa tcaaggctga atctgtgttc ggtgctgttc agggttttcc agccctcttc
     3961 cgcccaggct ggcattacag ccagagccaa aatggcaatc agggtcgcga tcgcaagttt
     4021 tttcattctt cctcccggtt ggggttgtct ttataggtac agatatccct ttgggggatg
     4081 gttccgcctg cagatggtac tctcgcccgg gagcatcccc tcatcttttt ttactccgct
     4141 ttgcccaact gtccttgagg gtaacgatgc ggttgaagac aggtttttcc tgagtgtaat
     4201 cgtcatccgc gcagaaatag ccctggcgca agaactggaa cctttcgccc tgaacagctt
     4261 tagccagcga gggttcaagc ttgcagcctg taagcacttg cagcgatgcg ggattcaggt
     4321 aatcgcgata gtccttgccc tcttccattg cattgaagtc ggcaatggtg aaaagctggt
     4381 cgtagagccg cacttcggca tcaatggcat gggcggcact cacccagtgg atggtgcctt
     4441 tcaccttgcg ttcgtcagat gtccagccac ctcttgattc tggatcgtgg ctgcagacaa
     4501 tctcggtaac gcggccttcc gtgtctttca ccacctcccg gcaggtgacg tagtaagcat
     4561 gtttgaggtg cacttcagct cccggcgaaa ggcggaacca gcctttgaca gggttttcgc
     4621 aaaaatcacc acgctcgatg tagagatgtt ttccgaacgg caccaaacgc ttcccggcgc
     4681 ctggatcttc agggttgttc tcagcttcca gttcttcgaa tttgccatcg ggatagttct
     4741 cgatggtcag cttcagcggc tccagcacca ccatcacccg gtttgcgcgt ttgttcaggt
     4801 cttctcgcac acagaactgg agaagctcga aatccacgat ggaagtggct tttgagacac
     4861 caatgcggtc ggcgaattcc cttatcgctt ctggcgaaaa gcctctgcgc ctcatcccgg
     4921 ctatggttgg catgcgcgga tcgtcccagc cgctcaccgc tccgctttcc accagttcca
     4981 gcagacggcg tttgctcatc acggtgtagc tcaggttcag cctggcgaac tcaatctgtt
     5041 gggggtggca gggcaccggc agttggtcca gaaaccagtc gtagagcggc ctgtggtctt
     5101 cgaactcgag ggtgcagatg gagtgggtga tgccttcgat ggcgtctgaa atgcagtggg
     5161 tataatcgta catgggataa atcacccatt ttattccggt gcgatggtgc tcggctttct
     5221 tgatgcggta gatcacggga tcgcgcatgt tcaggtttgg cgatgccata tctattttgg
     5281 cgcgcaggga ttttgtccct tcctcgaact ccccggctcg catgcggcga aagaggtcca
     5341 gattttcttc cacgctccgt tcgcggtatg ggctgtcatc accaggaact gtcagggtgc
     5401 cgcggtgggc gcggatttct tcctgactga ggtcgcaaac gtaagccagg cctttctgga
     5461 taaggatctc agcatattcg taaagctggt cgaaatagtc tgaagcgtaa aaaagattgt
     5521 cctgccaatc aaaacccagc caacgcacat ccgccatgat ggaatccaca tactccacat
     5581 cttccttgat ggggttggtg tcgtcgaaac gcagatggca gactccaccg tagtctctgg
     5641 caaggccgaa attgaggcag atcgatttgg cgtgcccgat atgcaggtaa ccgttcggtt
     5701 ctgggggaaa gcgggtcacc actttgctgt gtttgctgct ggccaaatcc ttgtctatta
     5761 tcgtacggat gaagttcgat atcacagttt tttcgctgat ttccatataa tctcgcatat
     5821 tagatctaat tgcacaccat tatttttggc ctgcctcacg agtcaaggga attgcgagaa
     5881 attgcctcta agcctgaccc agccaaaaaa agcttgtcaa aaatctgcct gcctcattag
     5941 tggcttcggc gtcaggggta aatacccctc gcgaacaggc aaagtgaaaa gattacttat
     6001 cctcagcgac ctatacccca cctgtcagga cagccagaag gggattttcg ttaaacacca
     6061 ggttgacgag ctggcccact attaccgggt tttggtgatc gctttccata ttggcggcag
     6121 tttcaaggtg gagcgttaca tccaaggcca ggtttcagcc tgctccatca cctatcctgc
     6181 cctgaaggtc ccgtttcttt ctgcctttgc cagcttcccg gtttttgccc tgcccgtcgc
     6241 aagacaggtt tgttctcgct tccggcctga cctcatccat gttcacgatt tccgccacat
     6301 cccggaattg ttctggctaa aaacttggtt ggacaggctg aagcagccca agatactcac
     6361 actacacaac attcgcaccc accctgaccg cctgggcgga aaccggttgc tgccctttta
     6421 tcgtaagacc atgccgaaag cattcgcccg ctgggaccat gttttcaccg tcaacaagcg
     6481 gctgcgtgat tggctgctgc cctggcagga tgctgacagg atcagcgttt tgggtaacgc
     6541 catcggtccc gcaccggact gccgggaaga ggaactcatt ccgattcggt ctggattgcg
     6601 aaaagacagc tacaagcttt tatccgttgg aaatctcacc ccggaaaagg gcttctcata
     6661 tctgatagat gctgtaaaaa tccttacgga caaagggctt gaccttcagt tggtaattgt
     6721 tggcaaagga actcagagag cctctctgaa agcacagatc agtgctctgg gcctggatgg
     6781 catagttcgt ctcaccggag gtcttaataa ccatattgtc cgtaacgttt atcagtattt
     6841 cgacctcttt gtgttgccca gttacagtga gacttttggc atcgtttttc tggaagcgat
     6901 ggatgccggc ctgccggtgc ttggcgtcga gggtgaaggt atccatggcc tctttgaaca
     6961 aggccgggaa gcgcttttcg cccaacctcg cgacagcatc agcctgtcaa actcaatcgc
     7021 tagattcatc caggaaccgg aacttgctac ccagcttgcc catgcaggaa aaatgagggt
     7081 caataaggat ttcatgatgc ccgggttgat aggccgggtg agggaagtgt atgaacaaca
     7141 ataaa
//
LOCUS       DAOOWR010000044         7124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10555_length_7124_cov_3.670958, whole genome shotgun sequence.
ACCESSION   DAOOWR010000044 DAOOWR010000000
VERSION     DAOOWR010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10555_length_7124_cov_3.670958"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLB85_02010"
     CDS             complement(<1..192)
                     /locus_tag="PLB85_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU72130.1"
                     /translation="MTDHIDISELKEYLGKEIQGFYLVAEKELRDGRNDQYLRLRLQD
                     RSGTIAGNVWKDAKNISESF"
     gene            complement(199..2097)
                     /locus_tag="PLB85_02015"
     CDS             complement(199..2097)
                     /locus_tag="PLB85_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72131.1"
                     /translation="MKYGLILALSVLYSMSFALGILHGSGQDVWPAADREYNFAYHQG
                     SDDYHFYGSNIWAVRFNFTEAYPANYNSEFQVTKALLWLPQTGDSVRVELFSDYYGGP
                     GASLAWAKVPVNSNQVEVPFSNPVQGDSLWLVVTYATNFANRFVAASAGGGEHSYYWN
                     TNSINPYFQSLATAGFNAELLFGLAGDFVLSNFDLELVDFDLEGDLQPRQQTGPGFTL
                     YNHSDLTVSSATVTVTVNSPGNTFTRADTIAISEPIPPRSEYVFGPQSPGYSEHQFSL
                     PDQPMQLKVRAVARALADTLAAGNNLILLNRFSFADPYPIWLAENFLREGGSAQITTN
                     QDPFSFPDIHILNYFPILSDSLANVAAQIRFNWYSFNTLPRTAVNGDLRLNGFSSGYG
                     TQYEQHCLAARDLKSFVSASECRFEHIAQNDLLSGELTLANDNTLLYNIATEYNLINS
                     TRLSVGLFKKVIFDGAERFVIDRWITHGAPLSGPLGQGEELRLNFNLSLSDLSLDELA
                     QNYRLYYWLQLSGGGRILYSAYSDFTNVVAVQDEVQPVPVLRVSPNPLRGDGWLKISL
                     DKGQKLGRVRIYNLRGQKILDLEGNKAELTLAGSEFPASGIYLLRVQVPLPEGGTATV
                     NKRINIIK"
     gene            complement(2094..2996)
                     /locus_tag="PLB85_02020"
     CDS             complement(2094..2996)
                     /locus_tag="PLB85_02020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU72132.1"
                     /translation="MKGSVLSIGNFDGLHLGHRKLLGELTALARRLKLRSVVITYDNH
                     PSQVLDRDLHSLLLLPPQQKARMLKELGVDEVTLLHFDEELSRVSAEDFLRDQIVGLF
                     EPTALVLGYDSHFGHQRRGDYAFLQEQQTLYGYQLHYVEPLQNGDRVVSSTLIREILI
                     QGNLVGANRLLGAPYTIYGKVVGGSALGRDLGFPTANLRLEEARQILPKNGVYLSRVR
                     LGGESFFGLTNIGSSPTLKHSEIVAVETHILDFDRQIYGVPLKVELLRYFREEKMFTG
                     TNALREAMLQDLARARELVAEGKL"
     gene            complement(3001..3864)
                     /gene="truB"
                     /locus_tag="PLB85_02025"
     CDS             complement(3001..3864)
                     /gene="truB"
                     /locus_tag="PLB85_02025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOU72133.1"
                     /translation="MPEPRGFLLIDKPSGCSSFDVVRGLRKLTGIRRIGHTGTLDPFA
                     TGLLICALGSATRLCGYLEAEDKTYSATLRLGSQTNTGDTEGDFLREDGPIPDKLDEA
                     FLRERVFELKELSPPQHSALKVNGRPAYDYARKGEKLDLAPRPVRISKFGVLNYDPPD
                     LTYVCRVSKGTYIRSLSEYIAGCIGTVGHTVALRREAIGAVDLSEAWQLDELTPENYQ
                     AAFHPPRNLFTGFEFYQPRDAELAALRNGQGIGSEGKDTDRILLLDGNDTLLGVAKRS
                     SGFVQPVINLR"
     gene            complement(3857..4864)
                     /locus_tag="PLB85_02030"
     CDS             complement(3857..4864)
                     /locus_tag="PLB85_02030"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOU72134.1"
                     /translation="MKNSQKLHDKLLAQLIPGRKVGIMSHVEPDGDGFAASLALQLIL
                     KDRGLESEIIVDQDSHLERFAFLMEGASVLTYHEGMSYDLLIVLDCNSYSRVGERTSL
                     IRDAGHVILIDHHVPENGIIKTDYSFVDISAASAGAIVFRALQSEIAALPEALRIAVA
                     NCIYVTIINDTNNFINANTNAEVFRIAAELCELGISPAQLYKEYFLNHGALEMRYIGE
                     VLSTIELHNDGRVLYMYSTLEMQARNKLTADSIMNITRWVQGVRDIDIIAYLREEKPG
                     EYRLSLRSPRFDVNSVAVAYGGGGHVSASGANLRGDLDSLKAELLGNLDRTLRNLPAN
                     A"
     gene            complement(4959..5456)
                     /gene="rbfA"
                     /locus_tag="PLB85_02035"
     CDS             complement(4959..5456)
                     /gene="rbfA"
                     /locus_tag="PLB85_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU72135.1"
                     /translation="MKSHRIPRLQEELKKLFNIAISQKMRDPALDWVTITDVVLSKDL
                     RYAKIYFSHYNNPATHDSIREQLIKTSGFLKKQIAGAQIMRTIPELTFFYDETEDRAE
                     AVEVLLASLKQRYDDDDDAYDPDIDLEDYLDDDDIYDVDEDEELYNEFIQEEEEEEEE
                     KEKDQ"
     gene            complement(5456..>7124)
                     /gene="infB"
                     /locus_tag="PLB85_02040"
     CDS             complement(5456..>7124)
                     /gene="infB"
                     /locus_tag="PLB85_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU72136.1"
                     /translation="KFFAMGQLVTINQRLDRDSLEMVCDEFKVPFSFEDEYGTDVLEG
                     NKDLYAEVEEEPRPPVVTIMGHVDHGKTSILDYIRNTNIVAGESGGITQHIGAYQIEI
                     NKNKITFLDTPGHEAFTAMRARGANITDIAVIVVAANEGLKPQTREAIDHAKAAGVNL
                     IIAINKIDLPDANVDRVVAQLLEYGVLLEQYGGEVPWCKTSVVTGEGILNLIELILLT
                     SELMELKARRKMPGLAVVIEAAKDTRMGSVATILVQEGTLSKGDIIVCGATHGRIRKI
                     ENERGQDIKILNPSDVGRIYGLNEAPKAGDFLNQVDNEKTARSISSERNQIRLEREKF
                     QNKSSLQNIFARIKEEAINELNIILKTDTDGSAEALADSLEKLSNEEVQVNIIHKSVG
                     GINEADVSLASASNAVILGFHVRASHLARKLAEEEGVEIKLYQVIYDTIEDVRHSLEG
                     MLKPTIEESVIGSATVRQVFKIKKVGTVAGCQVDRGVIKRGCKVRLFRNDVQVTEDDL
                     SSLKHYADDVAEVKAGTECGLSLANFSDIKEGDVLECYVLEEVAKKL"
BASE COUNT         1438 a         1967 c         2010 g         1709 t
ORIGIN      
        1 gaaagattcg ctgatgtttt tggcgtcctt ccagacgttg ccggcgatgg tgccgctgcg
       61 gtcctgcaaa cgcaggcgca gatactggtc gttcctgccg tcgcgcagtt ccttttcagc
      121 caccagatag aagccttgga tctctttgcc gagatattcc tttaattctg agatgtcgat
      181 atgatcagtc atttgggctc actttataat gtttatcctt ttattaacgg tggcagttcc
      241 gccctctggc agggggacct gaacccggag caggtaaatc cccgaggcgg gaaattcgct
      301 tccagccaaa gtcagttccg ctttgttgcc ttccaggtcc agaatcttct gtccgcgcag
      361 gttgtagatt ctcacccggc ccagtttctg tcccttgtcc aggctgattt tcagccagcc
      421 gtcgcctctg agcggattgg ggctcactct cagcacaggg accggctgga cctcgtcctg
      481 gacggcaacc acattggtga aatcactata ggcggaatag agaatcctgc cgccgccgga
      541 aagctgcagc cagtagtaga ggcggtagtt ttgggccagt tcgtccaggg aaaggtcgct
      601 gagtgacagg ttgaagttca gccttaattc ttcgccctgg cccagagggc cgctcagagg
      661 ggctccgtgg gttatccagc ggtcgatcac gaagcgctcg gcgccgtcaa agatgacttt
      721 tttgaagagc cctacgctca ggcgggtgga attgatcagg ttatactcgg tggcgatgtt
      781 gtagagcagg gtattgtcgt tggcaagggt cagttccccg ctgagcaggt cattctgtgc
      841 aatgtgctca aaccggcatt ccgaggccga cacgaatgat ttaaggtccc gcgcggccag
      901 gcagtgctgt tcatactgag tgccgtaacc cgacgaaaac ccgttcaggc gcaaatcccc
      961 gtttaccgca gtgcggggca gggtgttgaa gctgtaccag ttgaacctga tttgcgcggc
     1021 cacgttggcc agggagtcgc tgaggatggg gaaatagttc aatatatgga tgtctggaaa
     1081 agaaaaagga tcctggttgg tggtgatctg ggccgaaccg ccctcgcgca ggaaattctc
     1141 cgccagccag atgggataag ggtcagcgaa gctgaagcgg ttcagcaaga tcaggttgtt
     1201 gcctgccgcc agggtatccg ccaaagcgcg tgccacagcg cggaccttaa gctgcatcgg
     1261 ctggtcagga aggctaaact ggtgttcgga gtatccgggg ctttgagggc cgaacacata
     1321 ttctgagcgg ggcggaatcg gttcgctgat ggcgatggtg tctgcccggg tgaaggtgtt
     1381 tcccggcgaa ttgaccgtta cggtcacggt cgcgctgctc acggtgaggt cggaatggtt
     1441 gtagagcgtg aatcccgggc cggtttgctg ccggggttgg agatcgccct ccaggtcgaa
     1501 atccacgagt tccagatcaa agttgctgag cacaaaatcc cccgccaggc caaagagcag
     1561 ttccgcgttg aagcctgccg tggccaggct ttggaaatag gggttgatgc tgtttgtgtt
     1621 ccagtaatag ctgtgctcgc cccctccggc ggaagctgcc acaaagcggt tggcgaaatt
     1681 ggtggcatag gttaccacca gccacaggga atcaccctgc acgggattcg agaaaggaac
     1741 ctctacctga tttgagttca cgggcacctt agcccaagcc aggctggctc ccgggcctcc
     1801 ataatagtcc gaaaacaatt ccacccgcac cgaatccccc gtttgaggca gccaaagcag
     1861 cgccttggta acttggaatt cgctgttgta gttggctgga taggcctccg tgaaattgaa
     1921 gcggaccgcc cagatgttgg agccgtaaaa atgatagtcg tcgctgcctt ggtgataggc
     1981 aaagttgtat tccctgtcgg cggcaggcca aacgtcctgt ccgctgccgt gaaggatccc
     2041 tagggcgaag gacatgctgt agaggacact cagggcgagg atcagaccgt atttcacagc
     2101 ttcccctccg cgaccagttc cctggcccgg gccagatcct gaagcatggc ctcccgcaag
     2161 gcgttggttc ctgtgaacat cttttcctcc ctgaaatagc gcagcaattc cactttaagc
     2221 ggaacaccgt agatctgacg gtcaaaatcc agaatatggg tctccaccgc cacaatttcg
     2281 ctgtgtttca aagttgggct ggagccaata tttgtcaatc caaaaaaact ttccccgccc
     2341 agacgcaccc ggctgagata gaccccgttc tttggcagaa tctgccgggc ttcctccaaa
     2401 cgcaggttgg ctgtgggaaa gcccaggtcg cgccccaggg cggaaccgcc taccaccttg
     2461 ccataaatgg tgtagggggc tccgagcaat cggtttgccc cgaccagatt gccctggatg
     2521 agtatttccc ggatcagggt gctgctaacc acccggtcgc cgttttgaag cggctccacg
     2581 taatgcaact gatagccgta gagggtttgc tgttcctgta aaaaagcgta gtcgcccctc
     2641 cgctgatggc caaaatggga atcataaccc agcaccagag ctgtgggctc aaaaaggccg
     2701 acgatctggt cgcgaagaaa gtcttccgcg gatacccggg aaagttcttc atcaaaatgg
     2761 agcagcgtca cctcatccac ccccagttct ttcagcattc tggctttctg ctgcggaggc
     2821 agcaacagga gggaatgcag gtcacgatca agcacctggg agggatggtt gtcataggtg
     2881 atcaccacgc ttctgagctt gagccggcga gccagtgcgg tcagctcgcc cagcagcttg
     2941 cggtggccca gatgcagtcc gtcgaaattt ccgatgctta aaacgctgcc tttcataaag
     3001 tcacctgagg tttatgacgg gctggacaaa gcctgagctt cgtttggcca caccaagcaa
     3061 tgtgtcattg ccgtccagca gtaggatgcg gtccgtgtcc ttgccttcgc tgccaatacc
     3121 ctgtccgttc ctcagcgcgg caagttcggc atcccgcggt tggtagaatt caaagcccgt
     3181 gaacagattt cggggcgggt ggaaagcagc ctgataattc tccggcgtga gctcgtccag
     3241 ttgccaggcc tctgacagat caaccgctcc gatggcctct ctccgcaagg cgaccgtgtg
     3301 tcccactgtg cctatacagc cggcgatata ttcactcaag ctgcggatat aggtcccctt
     3361 ggaaacacgg caaacatagg tcaaatcggg gggatcgtag ttcaagacgc cgaatttact
     3421 gattcggacg ggccgtgggg caaggtccag cttttcaccc ttgcgggcgt agtcataagc
     3481 cggccggcca ttcactttca gcgccgaatg ctgcggaggg ctcagttcct tcagttcaaa
     3541 cacccgttca cgcagaaaag cttcgtccag cttgtccggg atggggccat cttcacgcag
     3601 gaaatctccc tctgtatcgc cggtgttggt ctggctgccc agtctcagag tggctgaata
     3661 ggttttatcc tccgcctcaa ggtatccgca aagccgggtg gcggagccaa gcgcgcagat
     3721 cagcagcccg gtagcaaaag gatcaagggt accggtatgt cctatcctcc tgatcccggt
     3781 cagctttcgc agtccgcgta ccacgtcgaa ggagctgcag ccggacggtt tgtcgatcag
     3841 caggaatcct ctcggttcag gcattcgcag gtagattgcg caaagtccgg tccaaattgc
     3901 ccagcaattc ggccttgagc gagtccagat caccgcgcaa gttggccccg gaagcgctca
     3961 cgtgacctcc gcctccatag gcgacggcaa cggagttaac gtcgaatctg ggggaacgca
     4021 ggctgagcct gtattcgccg ggcttctctt ccctcaggta ggcaatgatg tcgatatcgc
     4081 gaacaccctg cacccagcgg gtgatgttca tgatcgagtc ggcggtgagc ttgttgcggg
     4141 cctgcatttc cagagtggaa tacatataga ggacgcgacc atcattgtgc agctcgatgg
     4201 tggaaagaac ttcgccgatg tagcgcatct ccagggctcc gtgattgagg aaatattcct
     4261 tgtaaagctg tgcgggagag atgcccaatt cacagagttc cgccgcgatg cggaaaacct
     4321 cggcatttgt gttggcattg ataaagttgt tggtgtcgtt gatgatcgtt acatagatgc
     4381 agttggccac cgcgatcctc aaagcctccg gcagggcggc tatttcgctt tgcagagccc
     4441 tgaaaactat cgccccggca gaagccgcgg agatatccac aaagctgtaa tcggttttga
     4501 taatgccgtt ttccggaacg tgatggtcaa taaggatcac gtggccggca tcccggatga
     4561 ggcttgtgcg ttcgcccacc cgactgtagc tgttgcaatc cagcacaatc agcagatcgt
     4621 agctcatgcc ctcatggtaa gtgaggaccg aagctccctc catcaggaaa gcgaagcgtt
     4681 ccagatgcga atcctgatcc acgatgattt cgctttcaag gcccctgtcc tttaggatca
     4741 gttgcaaggc cagactggcc gcgaagccgt caccgtccgg ttcaacgtgg gacatgatgc
     4801 ccactttccg gcctggtatc agttgggcta gcagtttatc gtgcaatttc tgggaatttt
     4861 tcattttgat gttccccaca tcagccccac tcttgtccca cagccccatc cggagcgcgc
     4921 ttccgcgggg accgctctgg gttcaggggc cggcgggttt attggtcctt ttccttttcc
     4981 tcttcctctt cctcttcttc ctggatgaac tcgttgtaga gttcctcgtc ttcgtccacg
     5041 tcgtaaatgt catcatcgtc cagatagtct tccaagtcta tatctgggtc ataagcatca
     5101 tcatcgtcgt cgtatctctg cttgagtgag gccagcaaaa cttctaccgc ctcggccctg
     5161 tcctctgtct cgtcatagaa gaaggtcagc tcgggaatcg ttctcatgat ctgtgctcct
     5221 gcgatctgtt tttttaggaa ccccgacgtc ttgatcagtt gttccctgat gctgtcgtga
     5281 gtagcggggt tgttgtaatg agaaaagtat atcttggcat acctgaggtc tttggacaag
     5341 accacatccg taatagtcac ccagtccagc gcggggtccc gcattttctg gctgatggcg
     5401 atgttgaaaa gctttttcag ctcttcctgc aaacggggta ttcggtgtga tttcatcaga
     5461 gtttcttggc cacctcttca agcacataac attccaaaac gtcgccttcc ttgatatcgg
     5521 agaaattggc cagggaaagg ccacactccg ttcccgcctt cacttcagcc acgtcgtcgg
     5581 cgtagtgttt cagcgaggac aggtcgtctt ccgtcacctg cacgtcgttg cggaaaaggc
     5641 gcactttgca gccccttttg atcactccac gatccacctg gcagccggcg acggtgccca
     5701 ctttcttgat cttgaagact tggcgaacgg tggcggagcc gattacggac tcttcgattg
     5761 tgggtttgag cattccctcg agcgaatggc gtacgtcttc gatggtatcg tagatcacct
     5821 gatacagttt gatttccacc ccttcttcct ccgccagttt gcgggcgaga tggctggccc
     5881 tgacgtggaa gcccaaaatg actgcgttgg aggccgaggc caagctaacg tccgcctcat
     5941 tgatgccgcc cacgctcttg tggatgatgt tcacctgcac ttcttcgttg gaaagcttct
     6001 ccagcgaatc agccagggct tcggcggagc cgtcggtatc agttttcagg atgatattga
     6061 gctcgttgat agcttcttcc ttgatgcggg cgaagatatt ctgcagcgag gatttgtttt
     6121 ggaatttctc gcgttcaagg cggatctggt tccgttcgga gctgatgctg cgggcggttt
     6181 tttcgttgtc cacctgattg aggaagtcgc cggccttcgg agcctcgtta agaccgtaaa
     6241 tcctgccaac atctgaggga ttaaggattt ttatgtcctg gccgcgttcg ttctcaattt
     6301 tgcggattct gccatgggtg gcgccgcaaa cgatgatgtc ccccttgctc agggtgcctt
     6361 cctgtacgag gatggtggcc actgaaccca tgcgggtgtc tttggcggct tcgataacca
     6421 cagccaggcc cggcattttc cggcgggctt tgagttccat cagttccgaa gtgagcagga
     6481 taagttcgat caggttcagg atgccttccc cggtaaccac agaggttttg caccagggca
     6541 cctctccccc gtactgctcc agcaaaacgc cgtattccag caactgcgcg acaacgcggt
     6601 ccacgttggc gtccggcagg tcgatcttgt tgatggcgat gatcaggttc accccggcgg
     6661 ctttggcgtg gtcgatggcc tcgcgggtct ggggtttcaa gccttcgttg gccgccacca
     6721 cgatcacggc gatgtcggta atgttcgccc ccctggcccg catggcggtg aaggcttcgt
     6781 ggccgggcgt gtccaggaag gtgatcttgt tcttgttgat ctcgatctga taggcgccga
     6841 tgtgctgggt gatgcctccg gattcccccg ccacgatgtt tgtgttgcgg atgtagtcca
     6901 gaatcgaggt cttgccgtgg tcaacgtgtc ccataatggt caccacgggc ggccggggtt
     6961 cttcttccac ttcagcgtac aaatctttgt tcccttccag aacgtccgtg ccgtactcat
     7021 cttcaaagct gaatggtact ttgaactcat cgcagaccat ttccagcgaa tcgcggtcca
     7081 gacgctggtt tatcgtgacc aactggccca tggcgaaaaa cttg
//
LOCUS       DAOOWR010000045         7052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10714_length_7052_cov_4.324282, whole genome shotgun sequence.
ACCESSION   DAOOWR010000045 DAOOWR010000000
VERSION     DAOOWR010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10714_length_7052_cov_4.324282"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLB85_02045"
     CDS             complement(<1..498)
                     /locus_tag="PLB85_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72137.1"
                     /translation="MSDRDVQNLLHRAEALMKTNWIHAVQLLIQAAEENPQDPRPLIA
                     LGDYYQRRQLYDKAVKSYQSALKLRPGDNQLKLIIGNTYFAQGEYQLAIVYYDQITDP
                     HSDVRFNKALALAYLGRNRESIEIMKNLMSVIDNNPFIYFLLIEQLLRVEDYEAAQSY
                     IARAEA"
     gene            complement(630..3782)
                     /locus_tag="PLB85_02050"
     CDS             complement(630..3782)
                     /locus_tag="PLB85_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU72138.1"
                     /translation="MKHIITLILALAATLGWALEPEFASDPAISPDGEQVCFVFDNDL
                     WLVSYNGGDAHRLTATEAGEWGPCWSPDGRWIAFNSNREGITLPYLIEPTSGITRLII
                     RESFTVNDWFADGQSLLCTRYSPSFGRSFFRLNLDGSRPVLLAEIGDSYATLAPDNKS
                     LVFNRDGNPYREAYLGSIAGELWSIDLASKKYTRLTHTEFTERYPRYSHLDSALYFCA
                     SDGQRFQIFRADNGNFDSPRQLTDFPLWSARDLSIARQNDRIVFEHFNEIWRWHPVTG
                     TSKLEININSDLWRPDLRREKMKDSFEAFAISDDDLLVGFKYKYDTFFIPRKGGEVKQ
                     ISRDQSSIGDLAFLKDNRTLALQMMDKGINKLYTVTCDSLMQLSQVEWFGADSLDVES
                     IMRDPSGKWVVRYGDNRLSGRIAVADSSLTDLRPLNARRPIASNFAINSSGDYAVYAT
                     TREDVWMRELWLYDFAADAHIKIMNDDSWIRGLAWTPDDRSILIGRSNGIYRLDLVPR
                     DEYEYETDNWKEIFNTTTGEDGEELIVSDKVLEVTIDAETDTLAMATEDEPTPRKELN
                     IVWEGIEKRLSRVINEPERWLRVERVIDDSTFYYISDGYDEDSSLKLKKANMFGDNVK
                     EVQDLGKNASSLRWVREKLYYLQGGKIRWYDLDKRSKGELSTEFDYTYSTSLLNTRVF
                     EEVWGVFGLNFYDPNMHGQNWQALYELYHPYANRARSIEDISSIVNEMIGDINASHTG
                     FYPRREGYSPRGGQPAYLGVEFDYREVLPQGIRLRAVYPTSRLATFYGIREGDILTHI
                     DGSPITPATPLDSLLADKTGKRIRLNFIANGEEKEAVQEGLNWEKNRALLYEYKVSRS
                     RTEVDQRTGGRVGYIHIPAMGDNNFTNFQRELFRDNADKEALIIDVRGNTGGHVHDQI
                     ISLLNKTPYALSTSRRYSGQQNLEPRRAWTRPSIVLADENSFSDGEIFPIVYQELKLG
                     KVVGKPSSGSVIGTWEYNLLDGSSMRLPGSGWYKLDGTNMEGSGVQPDIVVEISPEDE
                     IAGRDTQLWRAIEEILKELP"
     gene            complement(3795..5081)
                     /locus_tag="PLB85_02055"
     CDS             complement(3795..5081)
                     /locus_tag="PLB85_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOU72139.1"
                     /translation="MNKILTNRAGTLRLELRDDRLSAWLTIRSHGRLIDEQDILDLIE
                     EAGIKTGFEEADRYMRKHGLEKDFDIPFPIAMCDRVKGESKLNYFFDLNQAKNFTGRV
                     KPEELAILTCIEAGTVIADYSSNIFDRQGSIYDIYGEMLQDEEFDLEAAGQVAGDNVT
                     FSLDKHHFVTERKGFVSVDENGRIGIIEKLVVEGDIRDAMEELRSPADLEVWGDIINS
                     TLFCAGNVELRGDIINSTLNCRGDLVLCGEIRDCCQPGVEIGGNLCCKGIKASRVLCQ
                     GSISFEDTVEGSEIAADGGLEAEKGSLRGGRIECGGNVVLRELGDPSGGETRLEITIG
                     PYHKALLMQMTKELIRLKETPGENQSLIDDLNAQIKASETELDGELNRFLNRPSDERL
                     GLRVLGTVHPPVLARVLKHEYKVQSRQNGLELWEKD"
     gene            complement(5074..5769)
                     /locus_tag="PLB85_02060"
     CDS             complement(5074..5769)
                     /locus_tag="PLB85_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOU72140.1"
                     /translation="MKTWLHLGEKTPDYRSVMSAQGDIRIGSKIIPKGRTVIAEQIAG
                     DGIHICSEADGGAGWGDHSQIAGVDALITSVPGQYLLVRTADCYPVLLQDAQNRAVAA
                     IHSGREGTRLNIVGKTVRVLQDEYGILPAEITAHIGAGICARHYEVDEATWESFNTSL
                     KEMGCAADAAPAGSRHLDLRLAIFRQLIAAGIPFYNIEQQFVCTLESAVHHSYRRDRT
                     SNRQMNLIGLEYE"
     gene            complement(5766..>7052)
                     /locus_tag="PLB85_02065"
     CDS             complement(5766..>7052)
                     /locus_tag="PLB85_02065"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU72141.1"
                     /translation="ISLTVRNYRIYFSGHFFSVIGIWVQRTTMSWFIYRLTNSAFLLG
                     LIGFLSMIPSLFVSPFAGAWADRWDRRKTLVLTQTLFMVQASLLAAGVLSGFINEDRW
                     WPLIVLALWQGIVEGIDAPFRQNFVLDLVSKRSLLPNAIATNSAMFNSARLIGPSIGG
                     AMIVAFGEGICFLISAVAYLAVIASLLTIRINYPPLKPSGVPILSKIKEGWSYSWHSL
                     PVRWLIANLGVYMLLAMSYSSIIPVFARDVLKGNAGTQGLLLTFAGVGALTSAFYMAG
                     RKTIKGLPYITAVLGCVANLALIGLAQSRSIVLSLVFMVFIGLGMTIQMSSTNTILQS
                     VVDPNMRGRVLSVYTMTFQSVMPFGSLLIGSLTRKLGPQTALSISAGICLLWSLNALR
                     NIPHLITHIQRMLVTNHNTEIYRPLKVTVMYPGVEG"
BASE COUNT         1496 a         1967 c         1918 g         1671 t
ORIGIN      
        1 ggcttcagca cgggcaatgt agctctgcgc ggcttcgtaa tcctccaccc gcagcaactg
       61 ttcgatcaac aggaaataga tgaagggatt gttgtctatc accgacatca ggttcttcat
      121 gatctcgatg ctttcgcggt tccggcccag ataggccagc gccagggctt tgttgaagcg
      181 cacatccgag tgcggatcag tgatctggtc gtaatatacg atcgccagct gatactcgcc
      241 ctgggcgaaa taggtattgc caattatcag ctttagctga ttgtcgccag gtctcagttt
      301 caaagcggat tgatagctct tcacggcttt gtcgtaaagc tggcgtcgct ggtaataatc
      361 acccaaagcg atcaagggac ggggatcctg gggattttcc tccgccgcct ggatcagcaa
      421 ctgcaccgcg tgaatccagt tagttttcat cagcgcttca gcgcgatgga gcaggttttg
      481 tacatcacgg tcggacatct ggattctcct gtgtcacttt cctgtcagcc tctgcgaatc
      541 tccagcccga caaagcggcc atgaatccct ttttggctgt gaggctcctg tagctcccaa
      601 gcttgtttgt ccaaaccatc cggagccgat catggcagtt ccttgaggat ttcttcgatg
      661 gcacgccaca gttgggtgtc gcgtccggcg atctcgtctt ccgggctgat ttccaccact
      721 atgtccggtt gtacgccgct gccttccatg ttggtgccgt ccagcttgta ccacccggag
      781 ccgggtaagc gcatggacga gccatccagc aaattatact cccacgtgcc gatcacggaa
      841 ccgctggagg gtttgcccac cactttgcca agcttaagct cctgatagac aatgggaaat
      901 atctcgccgt cggagaagga gttttcgtcg gccagcacga tgctggggcg cgtccatgcc
      961 cggcggggtt ccaggttttg ctgtccgcta tagcgccggg aagtggacag agcgtaggga
     1021 gttttattga gcagggagat gatctggtca tgcacatggc cgccggtatt gccgcgaacg
     1081 tcgatgatca gggcttcctt gtctgcgttg tcgcggaaaa gctcccgctg aaagttggta
     1141 aaattattgt cgcccatagc cgggatatgg atgtagccaa cccttccgcc ggtgcgttgg
     1201 tccacttcag tgcggctgcg gctaaccttg tattcgtata gcagagcgcg gttcttttcc
     1261 cagttcaagc cttcctgcac ggcttccttc tcctctccat tggcaatgaa attcaggcgg
     1321 atgcgcttgc cggtcttgtc tgccagcaat gagtccagcg gggtggcagg cgtgatgggg
     1381 ctgccgtcga tgtgggtgag tatgtcgcct tcccttatgc cataaaacgt tgccagacgc
     1441 gaggttggat aaacggctcg caggcggatt ccctgtggca atacctctct gtagtcaaat
     1501 tccacgccaa gataggcggg ttggcccccg cgcggagaat agccttccct acgaggataa
     1561 aacccagtgt gggaggcgtt tatgtcgccg atcatctcgt tcacgatgga actgatgtcc
     1621 tcgatggaac gggcccggtt ggcgtaagga tggtagagtt cgtagagcgc ctgccagttt
     1681 tggccatgca tgttcgggtc ataaaaattg aggccaaaca cgccccagac ctcttcaaaa
     1741 acccgggtgt tcagcaaact ggtgctgtaa gtataatcaa attccgtgct tagctcaccc
     1801 ttgctgcgtt tgtccagatc ataccagcga atctttccgc cttgcagata gtaaagcttc
     1861 tccctcaccc agcgcaggct ggaggcgttt ttgccaaggt cctggacctc cttgacatta
     1921 tccccgaaca tattggcctt tttgagtttg agactgctgt cttcatcata gccgtcagat
     1981 atgtagtaga atgtgctgtc atcaattacg cgttccactc gcagccagcg ctccggttca
     2041 ttgatcactc tagagagccg cttttcgatg ccctcccaaa ctatgttaag ttccttgcgc
     2101 ggagtgggtt cgtcctcagt tgccattgcc agggtgtcgg tttccgcatc aatcgtgact
     2161 tccagcacct tgtcgctaac gataagctct tccccatcct cgccggtggt agtgttgaaa
     2221 atctctttcc agttgtccgt ctcgtattca tattcgtcgc ggggaaccag gtcgaggcga
     2281 tagatgccgt tggaacggcc gatcaggatg gagcggtcat ctggggtcca agccaagcca
     2341 cggatccagg aatcgtcatt catgatcttg atatgggcgt cggctgcaaa atcgtaaagc
     2401 cagagctcac gcatccaaac atcctcgcgg gtggtggcgt aaacggcata gtctccactg
     2461 gaattgatgg caaagttgct ggcgatggga cgcctggcgt tcagaggacg tagatccgtc
     2521 aaactggaat ctgccaccgc gatcctgccg ctgagacggt tgtctccgta gcgaacgacc
     2581 cattttccgc tggggtcgcg cattatgctt tccacgtcca ggctgtccgc gccgaaccac
     2641 tccacttggc taagttgcat taggctgtca caagttactg tgtaaagctt gttgataccc
     2701 ttgtccatca tttgcaacgc aagggtgcgg ttgtctttca gaaaagccag gtcgccgatg
     2761 gagctttggt cgcggctgat ctgcttcacc tcgccgcctt tacgggggat gaagaaggta
     2821 tcgtatttgt atttaaaccc caccagcagg tcgtcatcag agatggcgaa ggcttcaaag
     2881 ctgtccttca ttttttcccg gcgcaggtcg ggacgccaga ggtcgctgtt gatgttgatc
     2941 tccagcttgg aagtccctgt cactggatgc cagcgccaga tttcgttaaa gtgctcaaat
     3001 acgatgcggt cgttttgcct ggcgatggat aagtcgcgcg cggaccagag tgggaaatcc
     3061 gttaattgcc tcggcgagtc aaaattgccg ttgtccgccc ggaaaatctg gaaacgttga
     3121 ccgtcggaag cgcagaagta gagcgcgcta tccagatggg aatagcgggg atagcgttca
     3181 gtaaattccg tatgggtgag ccgggtgtat ttcttactgg ccagatctat gctccaaagc
     3241 tcaccagcga tgctgcccag gtaggcttcg cggtaggggt taccgtcgcg gttgaaaacc
     3301 aggctcttgt tgtccggcgc cagagtggca taactgtcgc cgatttcagc cagcaacaca
     3361 ggtcggctgc cgtccagatt aagacggaaa aaggatctgc caaaactggg gctgtaccta
     3421 gtgcaaagca agctttggcc atcggcaaac caatcgttga cggtgaagct ttcacggatg
     3481 atgagtcggg tgattccact agtcggctca atcaaatagg gcaacgtaat gccttcacgg
     3541 ttggaattga aggcgatcca acgcccgtca ggagaccagc aaggccccca ttccccagcc
     3601 tcagttgcgg taagccgatg cgcgtcgccg ccgttgtagg ataccagcca cagatcgttg
     3661 tcaaaaacga agcaaacctg ctctccatca ggggagatgg ccgggtcgga agcgaattca
     3721 ggttccaaag cccagcccag agtggccgca agcgccagaa tcagcgttat tatatgtttc
     3781 atatccactg tcctttaatc tttttcccag agttccaagc cgttttgcct gctctggacc
     3841 ttatattcat gctttagcac acgcgccaac accggaggat gcacagttcc cagcacacgc
     3901 aagcccaacc tctcatcgct cgggcggttc aaaaatctgt tcagttcacc atccagctct
     3961 gtttcgctgg ctttgatctg ggcgttcagg tcgtcaatca ggctttggtt ttctcccggt
     4021 gtttccttca agcggatcag ttccttcgtc atctgcatca gcaacgcttt gtgatagggc
     4081 ccgatggtga tttccagcct ggtttcgcct ccagagggat cgcccagctc acgcaggacc
     4141 acatttcccc cacattctat ccggccaccg cgcaaggagc ctttttctgc ttccagcccg
     4201 ccgtcggccg cgatttcgct gccctcaacg gtgtcttcga agctgatact gccctggcac
     4261 aacacccggg aggctttgat ccctttacag cacaagtttc cacctatctc aacccccggc
     4321 tggcagcagt cccggatctc gccacagagg accagatcgc ctctgcaatt aagcgtggag
     4381 ttgataatgt caccccgcag ttcgacgttc ccggcgcaga acagtgtgct gttgatgatg
     4441 tcgccccaga cctccagatc agccggagag cgcagttctt ccatggcgtc acggatgtca
     4501 ccttccacca ccagtttttc aatgatcccg atgcggccgt tctcatccac gcttacaaag
     4561 cctttccgct cagttacaaa gtggtgtttg tctaggctga aggttacgtt gtctccggct
     4621 acctgcccag ccgcctccag gtcgaattcc tcgtcctgca gcatttcgcc gtagatatcg
     4681 taaatagagc cctggcgatc aaagatgttg ctgctgtaat ccgcgatcac ggtgccagct
     4741 tcgatacagg taaggatggc cagttcctcc ggtttcaccc tgccagtgaa gttcttagcc
     4801 tggttgaggt cgaaaaagta attcagcttg ctttctccct tcacgcggtc gcacatggca
     4861 atagggaagg ggatatcaaa atccttttcc aggccgtgtt tacgcatata tctgtctgct
     4921 tcttcaaagc ctgttttgat gccggcttct tcgatcaggt ccagaatgtc ctgctcgtcg
     4981 atcagcctgc cgtgagagcg gatggtaagc caggcagata gcctgtcatc gcgcagctcc
     5041 agtctcagag ttccagcccg gttggtgagg atcttattca tattcaagtc cgattaaatt
     5101 catctggcgg ttgctggtcc tgtcccgccg gtaggaatga tgcacagctg attccaatgt
     5161 gcaaacaaac tgctgttcga tgttgtaaaa aggtatcccg gccgcgatca gctgcctgaa
     5221 tatggccaga cggagatcca gatgacggga gcctgccggg gcagcgtcag cggcgcagcc
     5281 catttctttc aggctggtgt taaaactctc ccaggttgct tcatccacct cgtagtgccg
     5341 agcgcagatg ccagcgccga tatgggctgt aatctcggct ggcaaaatgc catattcatc
     5401 ctgaagcacc cgcaccgttt ttccgacgat attcagccgg gtgccttccc gtccgctgtg
     5461 gatggctgcc acagcgcggt tttgagcgtc ttgaagcagt acggggtagc agtcagcggt
     5521 acggaccagc agatattgac caggaacgga tgtgatcaaa gcgtcaaccc ccgcaatctg
     5581 ggaatggtcg ccccagccag cgcccccatc cgcttcagag caaatgtgga tcccgtcacc
     5641 ggcgatctgt tccgcgatca cggtcctccc cttggggata atcttgctgc cgatccggat
     5701 gtcgccctga gcgctcatta cgctgcgata atccggggtt ttttcgccca gatgcagcca
     5761 ggttttcatc cttccactcc tggatacatc accgtaacct tcagcgggcg gtagatttcg
     5821 gtattgtggt ttgtcactag catgcgctgg atgtgagtta tcaaatgcgg gatattgcgt
     5881 agcgcgttca gcgaccataa caggcagatg ccagcgctga tgctcaacgc ggtctgcggt
     5941 cccagcttac gggtgagtga gccaatcagc aggctgccga agggcatcac agactgaaag
     6001 gtcatggtgt aaacgctcag cacccgacca cgcatattgg gatcaaccac gctttgcaga
     6061 atggtgttgg tggagctcat ctgaatagtc atgcccagac caatgaagac catgaaaacc
     6121 aggctgagca cgatgcttcg gctctgagcc aggcctatca gggccagatt ggccacgcag
     6181 cccaacaccg ctgtgatgta gggcaggcct ttaatggtct tccgccctgc catgtaaaaa
     6241 gcgctggtca atgccccgac ccccgcgaag gtaagcagca ggccttgggt gcccgcattt
     6301 cctttcagca catcgcgcgc gaacacggga atgatggagc tatagctcat agcgaggagc
     6361 atgtacacgc ccaggttggc gatcagccaa cgcaccggca gactgtgcca tgaatagctc
     6421 caaccttctt tgatcttgct gaggatgggc actccgctgg gtttgagggg tggatagttg
     6481 atccggatgg tcagcagtga ggcaattacc gccagatagg ctactgcgct gatcagaaag
     6541 caaatgcctt cgccaaaggc tacgatcatc gccccaccga tggatgggcc aatcaaccgg
     6601 gcggaattga acatggcgga attggtggcg atggcgttgg gcagcaggct gcgcttggaa
     6661 accaggtcca gcacgaaatt ctgcctgaac ggtgcgtcaa tgccttccac gatgccctgc
     6721 cagagtgcca gcacgatcag cggccaccag cgatcctcgt tgatgaagcc gcttagcact
     6781 cccgctgcca acagactggc ctgcaccatg aataacgttt gcgtgagcac cagcgttttg
     6841 cggcggtccc agcggtcggc ccaagccccg gcgaaagggc tcacgaacag cgacggaatc
     6901 atgctcagga agccgatcag gccaagcaaa aaagcggaat ttgtgagccg gtagataaac
     6961 cagctcatgg ttgtacgctg cacccaaatt ccgatcaccg agaaaaagtg cccggaaaaa
     7021 tagatgcggt agttgcgaac ggtgaggctg at
//
LOCUS       DAOOWR010000046         7028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10757_length_7028_cov_3.829055, whole genome shotgun sequence.
ACCESSION   DAOOWR010000046 DAOOWR010000000
VERSION     DAOOWR010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10757_length_7028_cov_3.829055"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1127
                     /locus_tag="PLB85_02070"
     CDS             24..1127
                     /locus_tag="PLB85_02070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU72142.1"
                     /translation="MENRTWEYVSVFIMSWLLVYGLTPFIIKLAKALNFVDKPEARKI
                     HLTSVPYMGGLSVFIGFFLLCIYDVTISANRSFDPAMLGYLGGSMLIMLIGLIDDRFG
                     MNPIIKLIGQITVSLLFILTNFQFSELRDMFGSVYISLPVMVLWMVGLMNALNFLDNM
                     DGILSGIAGILGLGFFAFSLANTTGSNGAEMAFIGLISLSFAGSVLGFLPYNFNPAKI
                     FLGDAGSMFIGYFLSSMGILMARYAVLTRENNVFYLLPVLLLSYAIFDISLVSYTRKR
                     DGRHVMQGGRDHSTHRINTALGSIKITALIIYAINILIVLTTIIIFITGNQVLLLVST
                     VMLATFFIVFGRKLDQIPIVVPSNQKKSNKVEK"
     gene            1181..2587
                     /locus_tag="PLB85_02075"
     CDS             1181..2587
                     /locus_tag="PLB85_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72143.1"
                     /translation="MNNRLPILFTALLLVFASACEINKYNSAEDHYNNRRYAAAIQEL
                     DNYIKTGQNGALITRGEILRGQCYYELGLLALQRESYDLAIKFLKLSNSQEADQALGR
                     IYRDMADKALAQGNKRLSLDFVNAILREIPGSELSAEMLNRRIGFRLDTFIDHEGAWQ
                     DYMDLFDNYPNNAFEVAARKQILRIIPAKVDQARQLYNTGYYSEGLRILFELGKYPVV
                     ESSANNQMIAEAYIGQAESYLKGQNYLEADRFFRIAVQYDPGKKAQVDRRLEQVASLF
                     INRGNELVAQRDFENALIHYQKTYDIIPDYALANQAIARMNTIRENVARAREIQAQAE
                     KAELSGKYSEALNLYRQANNLDSQQEFSAKISQMQNLIAAQADPAAFARRVINEYRGG
                     ILNIRIQRQKQELLRAYNPNEIRDSGWKFMISSGQFKYEARYDLVTPTISYYYVWQVN
                     LRDRSITPLNKLSEKLME"
     gene            2590..3174
                     /locus_tag="PLB85_02080"
     CDS             2590..3174
                     /locus_tag="PLB85_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72144.1"
                     /translation="MRKTVFTLFFLILASCLLAQIKFDIESFADSTKYGWQDWRGRAA
                     YREDLLNRQEMLQLYEMESSSIGAAILKSALVPGFGQITSKAGTKGTIFLGAELLSLG
                     VSLYLYDRSNYYYNQYLTATQIDDIQQYYSLAQTPRYYSMLFLALGVVIWGYNIYDVI
                     LTTDNYNAQVWQDILQKHSKKAVSLGPEGIRVRF"
     gene            3185..3619
                     /locus_tag="PLB85_02085"
     CDS             3185..3619
                     /locus_tag="PLB85_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72145.1"
                     /translation="MKTKLILAALVLLLTAACLKHNNPLDPYDNPDIIVPDQVSGVQC
                     FASQAGSANKYVEVRWIANPALNTDGYYVFRGLAYNSDYAVVDTVATNFCNHGSEAWH
                     SVLPGDYWYKVSAFRDVYDGYGNFLGKLEGRRSEPRFIRVPA"
     gene            3626..5824
                     /locus_tag="PLB85_02090"
     CDS             3626..5824
                     /locus_tag="PLB85_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOU72146.1"
                     /translation="MCADFSKELNPQQLAVVTDNEGPLLVLAGAGSGKTRSIIYRCAW
                     LLKEKQVPPWNILVVTFTNKAARELQERLQNLLGFPVRSLWVGTFHHVCSRILRSEAA
                     SLPFGSNFSIYDQDDQASLLKKIYKEHNLDRQKFPIQRVLTRIGRYKNRLLLPEDLSS
                     THAVDSRGNRWQDDFENGVLRIYGLYQQKLLLNQALDFDDILLYTAKLLQDNSAVRAK
                     YGQMFRYVMIDEYQDTNQAQFEIVYQIAKDHQRVCVVGDDDQAIYSFRGATIRNILEF
                     EQDYSNVRSIRLEQNYRSTTGILDLANAIIRHNRRRHRKDLWSDLGKGEKPQLLNHQN
                     ETEEANQTAKMVVRLHAEGVPYGQIAVLYRTNAQSRVFEYAFLQEEIPHAIVGSLHFY
                     QRKEIRDLLAYLAVLSNPSDAESLLRIINEPPRGIGQTTVNRLLAHAAKTRGTLYQAL
                     QNSASIEEIGPSLKKKLVEFSEKLDDWRKLAREAPVLDLVKELMDYLDLVNLYKQSKD
                     PKDISRVENLIEFVGSVSEFSERWAEEHDTPPRLDDFLPFVALQTDMDRMDSGADSVR
                     LMTLHNAKGLEFGCVFIVGLEDELLPHRMSLDSHEAIEEERRLFYVGVTRAKTRLFLS
                     YAKCRRLYDTFYYTKPSQFLQELDESLFAGSNDQTDFTPAPRHRPKTRHRITEKDKQF
                     RIGQQVWHSEYGKGMVLSVDGIGSSARLTVSFVSGKLAKIIGTFVSTEPL"
     gene            5832..>7028
                     /locus_tag="PLB85_02095"
     CDS             5832..>7028
                     /locus_tag="PLB85_02095"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU72147.1"
                     /translation="MTLYERILPRLERSPFFQELATLPQSPERHQLHHLNQSARALAA
                     AHLWAKTGKNILIVSQDDIIAEDIWDDLCTLIGTASAHYLPDYEILPYEERSPHYSIR
                     AARMMCLHRVIEGKPAVYSLSIRALMRFLPSLENIARHVKTLRPGMEYPPEELMRNLV
                     FMGYEVEYQVNKVFQAARRGGILDIFSPPALKPVRVEYFGDEIITMRYFSPNTQRSEP
                     GEVPSYTVIPAREFCLDDVRATSPLMAKVKHTGFWEGIENQYSLLLDSLVTFADYFAP
                     GERLLLFSNYLYIQEEFEQLAEQVNSQYRRELKNTTKAKLSPPDRVLAGEEKLLELCR
                     PGSAVFLSQSEFILPFPARGHKAPCEPQPGLNGDLALLASSLKRMGAEGVDPVLLFDN
                     PSQAKRM"
BASE COUNT         1667 a         2055 c         1746 g         1560 t
ORIGIN      
        1 aatattatag atggagtatg aacatggaga ataggacatg ggaatacgtg agcgtgttca
       61 ttatgtcctg gttgctcgtt tacggactca cgcctttcat cattaagctt gccaaggcgc
      121 tgaacttcgt ggacaaaccc gaagccagaa agatacatct caccagcgtc ccatacatgg
      181 gcgggctgag tgtgttcatt ggttttttcc tgctctgcat ctatgatgtg accatatccg
      241 ctaaccgcag cttcgatccc gcgatgctgg gctacctggg cggttctatg ctgatcatgc
      301 tgatcggcct cattgatgac cgcttcggca tgaaccccat catcaaactg atcggacaaa
      361 tcactgtaag cctgcttttc attctcacga acttccagtt ttcggaatta cgggacatgt
      421 ttgggtcggt ctatatctcc cttccggtga tggttctttg gatggttggc ctgatgaacg
      481 ctctgaattt cctggacaac atggacggca tcctgagcgg aatcgcggga atccttggct
      541 tgggattttt tgccttcagc ttggccaaca ccactggctc gaatggagcg gagatggctt
      601 tcatcggctt gatatcgttg agtttcgcgg gcagcgtttt gggctttctg ccctacaact
      661 ttaaccccgc caaaatcttc ctgggcgacg cgggatctat gttcatcggc tattttctgt
      721 cctcgatggg catcctcatg gcccgctatg ctgtgctgac cagggaaaac aacgttttct
      781 acctattgcc cgttctgctg ctcagctacg ccattttcga catctccctg gtgagctaca
      841 cccgcaagcg cgatgggcgc cacgtgatgc agggcggcag ggaccattcc acccaccgca
      901 taaacactgc gcttggctcc atcaagatca ccgccctgat catctatgcc atcaacatcc
      961 tcatcgtgct caccactatc attatattca tcaccgggaa ccaagtgctg ctgctggttt
     1021 ccactgtgat gctggctact tttttcatag tctttggacg caaactggac cagataccga
     1081 tcgtggtgcc aagcaaccaa aaaaaaagca ataaagtgga aaaatgagcc gtcttatcag
     1141 aactggaaac acgggttctc gcaacaggct tggagagttt gtgaacaacc gtttgccgat
     1201 cctgttcact gcacttctcc tcgtgtttgc aagcgcctgc gaaataaaca aatacaatag
     1261 tgctgaagac cattacaaca atcggcgcta tgccgcggcg atccaggaac tggacaatta
     1321 catcaaaacc gggcaaaacg gcgccttgat cacccgcgga gaaattctgc gcggtcagtg
     1381 ctattatgag ttgggattgc tagccctgca acgcgaaagc tacgatttgg cgataaagtt
     1441 tttaaagctc tccaattcgc aagaagcgga ccaagctctt ggccgtattt accgtgacat
     1501 ggctgacaaa gctttggctc agggaaacaa gcggctttcg ctggatttcg tgaacgccat
     1561 cctgcgtgag attcccggct cggaactctc agcggaaatg ctaaaccgcc gaatcggctt
     1621 ccggctggac accttcatcg accatgaagg cgcctggcag gactacatgg acctcttcga
     1681 caattatcca aacaacgcct tcgaagtagc cgcccgcaaa caaatcttgc gcattatccc
     1741 tgctaaggtg gaccaagccc gtcagcttta caacaccggc tactacagcg aaggcttgcg
     1801 catcctcttt gaactgggca aatacccggt ggtggaaagc tccgccaaca accagatgat
     1861 cgccgaagcc tacatcggcc aggcggaaag ctatctgaag ggacaaaact atctggaggc
     1921 cgaccgcttc tttcgcatcg cagtgcagta tgatccgggt aaaaaagccc aggtggatag
     1981 gcgtctggag caagtagcct cgctcttcat caaccgtggc aatgaacttg tagcccagcg
     2041 cgatttcgag aacgccctga tccactatca gaaaacctac gacatcatac cggattatgc
     2101 tctggccaac caggccatcg cccggatgaa cactatcaga gaaaacgtcg cacgggccag
     2161 ggaaatacag gcgcaggccg aaaaagccga gctttccgga aaatattcag aggcgttgaa
     2221 cctctaccgc caggctaaca atctggatag tcagcaggag ttcagtgcca agatttccca
     2281 gatgcaaaac ctcattgccg ctcaggcgga tcccgccgcc tttgccagac gcgtgatcaa
     2341 cgaataccgc ggcggaatcc tcaacatccg catccaaagg caaaaacagg aattgctgcg
     2401 ggcttacaac ccaaacgaaa ttcgcgactc aggctggaag ttcatgatct cttccggcca
     2461 gttcaagtat gaagcccgct acgaccttgt aacccctacc atatcatatt attacgtttg
     2521 gcaagtgaat ctgagggacc gctctatcac ccccctgaac aagctttccg aaaaactgat
     2581 ggagtaagca tgcgcaagac ggttttcact ctgttctttc tgatcctggc atcatgtctg
     2641 cttgcccaga tcaagttcga catcgagtct tttgccgaca gcaccaaata cggttggcag
     2701 gattggcgcg gacgcgcggc ttaccgtgaa gacttgctca accgtcagga aatgctgcag
     2761 ctctacgaga tggaatccag ctcgataggt gccgccattc tcaaatctgc tctggtgccg
     2821 ggttttggcc agattaccag caaggccggg accaagggaa ccatcttcct gggcgcagaa
     2881 ctgctctcct tgggggtgtc actctacctc tacgaccgaa gcaattacta ttacaatcaa
     2941 tatctcaccg ccacccagat agacgatatt cagcagtatt acagcctggc ccagacgccc
     3001 aggtattact ccatgctctt tttggcgctg ggtgtggtga tttggggtta taacatttac
     3061 gacgtgatcc tcactacgga caactacaat gcccaagtct ggcaggatat cctgcaaaag
     3121 cattccaaaa aggccgtcag ccttggcccg gaggggatcc gggtgcgttt ttaggatggc
     3181 tccgatgaaa accaagctta ttcttgccgc cctggtcctg ctgctcacag cggcctgcct
     3241 caagcacaat aaccctctcg acccctatga taatccggat atcatcgtgc cggatcaggt
     3301 gagcggggtg caatgctttg cctcccaagc gggttcggca aataaatatg tcgaggtgcg
     3361 ctggatcgct aacccggccc tgaacaccga tggctactac gtttttcggg gactggccta
     3421 caattctgac tacgctgtgg tggacaccgt ggccaccaac ttctgcaacc acggctctga
     3481 agcgtggcat tcggttttgc ccggtgatta ctggtacaag gtttccgcgt tcagggatgt
     3541 ttacgacggt tacggcaact ttctgggcaa gctggaagga cggcgcagcg aaccccgctt
     3601 catcagagtt ccggcataat agggcatgtg tgcagatttt tccaaggagc tgaaccctca
     3661 gcagctcgcg gtcgttaccg ataatgaagg cccgcttttg gttctggcgg gcgctggcag
     3721 tggcaagaca cgctctatca tctaccgttg cgcctggctg ctaaaggaaa aacaggttcc
     3781 gccctggaac attctggtgg tcacgttcac gaacaaagcc gcccgcgaac tgcaggagcg
     3841 cctgcagaat ctgcttggtt ttcccgtccg ttcgctctgg gtgggcactt tccaccacgt
     3901 ttgttcgcgc atcctgcgca gcgaagcagc ttctctgcca ttcgggtcga acttttccat
     3961 ctacgatcag gatgaccagg cttcgctgct gaaaaagatt tataaagagc acaacctcga
     4021 caggcagaaa ttccccatcc agcgcgttct gactcgcatc ggacgctaca agaaccgcct
     4081 tcttctgcca gaagacctaa gctccaccca tgccgtggac agccgtggaa accgctggca
     4141 ggatgatttt gaaaacggcg tcctgcgcat ctatggcctc tatcagcaaa agttgctgct
     4201 caaccaggca ctggatttcg atgatatcct tctatacact gccaaactgc tgcaagacaa
     4261 ctccgctgtt cgagctaaat acggccagat gttccgctac gtgatgatcg acgagtatca
     4321 ggacacgaac caggcccaat ttgaaatcgt gtaccagatc gccaaagacc atcagcgcgt
     4381 ctgcgttgtg ggcgacgatg accaggcaat ttacagcttc cggggcgcca ccatccgcaa
     4441 catcctggaa tttgagcagg attacagcaa tgtccgctcc atccgcctgg aacagaatta
     4501 ccgcagcacc accggtatcc tggacctcgc caacgccatt atccgccaca accgccgccg
     4561 ccaccgcaag gacctctggt cggacctcgg caagggcgaa aaaccccaac tgctgaacca
     4621 tcagaacgag acggaagaag ctaaccagac cgccaaaatg gttgtcagac tgcatgcaga
     4681 gggcgttccc tacggccaga tagcagttct ttaccgcacc aatgcccagt ctagggtgtt
     4741 cgaatacgcc tttctgcagg aggaaatccc ccacgctatc gttggcagtc tccattttta
     4801 ccagcgcaag gaaatccgtg acctgctggc ctatttggct gtgctttcca atccctcgga
     4861 cgcagaaagc ctactccgca tcatcaacga gccgccccgg ggcatcggtc agaccactgt
     4921 gaatcgcctt cttgcccatg ccgccaaaac ccgcggcact ctttaccagg ccttgcagaa
     4981 ctcagcctcc atcgaagaaa ttggtcccag tctcaaaaag aaactggttg aattctccga
     5041 gaaactggat gactggcgca agctggcccg cgaagctccg gtactcgatt tggtgaaaga
     5101 gctgatggac tacctcgact tggtgaacct ctacaaacag agcaaagacc ccaaagacat
     5161 ttcccgggtg gaaaacctta tagagtttgt cggttccgtg agcgaatttt cggaacgttg
     5221 ggccgaagag cacgacacac cgccccgcct ggacgatttc ctgccctttg tggccctgca
     5281 gacagacatg gacaggatgg acagcggcgc tgactccgtg aggctgatga ccctgcacaa
     5341 cgccaagggc ctggaattcg gctgcgtttt catcgtaggc ttggaggacg agttgcttcc
     5401 ccaccgcatg agcttggaca gccacgaagc cattgaggag gagcgccggc tcttctatgt
     5461 gggggtgacc cgcgccaaaa ctagactgtt cctaagctac gccaaatgcc gtcgcctcta
     5521 cgacaccttt tactacacca agcccagcca gtttttgcag gaactcgacg aatcgctttt
     5581 tgccgggagc aacgaccaaa cagatttcac ccccgccccg aggcatcgcc ccaaaacgcg
     5641 ccaccggatc acggaaaaag acaaacagtt ccgcatcgga caacaggtct ggcactccga
     5701 atacggtaaa ggaatggtac tcagcgtgga tgggatcggt tcttccgctc gccttacggt
     5761 atccttcgtt tcgggcaagc tggccaagat catcggcacc tttgtgagca cggagcccct
     5821 ctgaagcagg tatgacgctc tacgaaagaa tcctccccag gctggaacgc tcgcccttct
     5881 ttcaggaact ggcaaccctc ccccaaagcc cagaacgcca ccagctccac catctgaatc
     5941 aaagtgcccg cgcccttgct gcggcccatc tttgggcaaa aaccggcaaa aacatcctga
     6001 tcgtttcgca agatgacatc atcgctgagg acatctggga cgacctctgc accctaattg
     6061 gaacagccag tgctcattac ttgccggatt atgagatttt gccgtacgag gaacgctcgc
     6121 cccactattc catccgcgct gccaggatga tgtgcctcca cagagtcatc gagggaaaac
     6181 ccgctgttta cagcctctcc atccgtgctc tgatgcgatt cttgccgtct ctggaaaaca
     6241 tcgctcgcca cgtgaaaacc ctgcgcccgg gcatggagta tccacccgag gaattgatgc
     6301 ggaaccttgt tttcatgggt tacgaagtgg agtatcaggt gaacaaggtc ttccaagctg
     6361 cccgtcgcgg tggcattttg gatatcttca gtcccccggc actcaagcct gtgcgcgtgg
     6421 aatactttgg cgacgaaatc ataaccatgc gctatttttc gccgaatacc cagcgttccg
     6481 aacccggcga ggttccctcc tacacggtaa ttcctgcccg cgaattctgt cttgacgatg
     6541 ttcgcgccac ttcccccctg atggcaaagg tcaaacacac aggcttctgg gagggcatcg
     6601 aaaaccaata cagcctgctg ttggacagct tggtcacttt cgccgattat tttgccccgg
     6661 gggaaaggct gctacttttc tcgaactatc tttacatcca ggaggagttt gaacaactgg
     6721 ccgaacaggt gaacagccaa taccggcgcg agctgaaaaa caccaccaaa gccaagctca
     6781 gccctccgga cagggttttg gcaggcgagg aaaaactgct ggagctttgc cgccccggaa
     6841 gcgcagtctt cctttcccaa agcgagttca tcctgccttt ccctgcccgt ggccacaaag
     6901 ccccctgcga gcctcaaccc ggcctgaacg gcgatctggc cctgctggcc tcctccctga
     6961 agaggatggg cgccgagggc gtcgatccag ttttgctctt cgacaacccc agccaggcca
     7021 agcgcatg
//
LOCUS       DAOOWR010000047         6999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10814_length_6999_cov_3.183324, whole genome shotgun sequence.
ACCESSION   DAOOWR010000047 DAOOWR010000000
VERSION     DAOOWR010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_6999_cov_3.183324"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /gene="ssrA"
                     /locus_tag="PLB85_02100"
     tmRNA           complement(<1..268)
                     /gene="ssrA"
                     /locus_tag="PLB85_02100"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(520..1422)
                     /locus_tag="PLB85_02105"
     CDS             complement(520..1422)
                     /locus_tag="PLB85_02105"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOU72148.1"
                     /translation="MKSLATSLGRLRLKSPVTVASGTFDPDYFELFDQDVLGAYTTKT
                     ITLAPKAGNPPPRLYETEAGLLNSIGLQNPGLKAWLNEDLPSLLDLLRIPLIVSFSGS
                     SEDEFLRILEGLEVQDGIAGYEVNVSCPNVAREGIAFGADPDVVFSLASRLSAITQRE
                     LIFKLSPNVTNIACIAQAAESGGASSLALINTLYGSAIDWKTGRFRISSPVCGYSGIG
                     IKPVALALCHKVAQVVTIPILALGGIHSWRDALEFFWAGASAIALGTAFYSDPLAAVK
                     LRDGLEKHLRENDLSLSDIIGKAL"
     gene            complement(1419..2159)
                     /locus_tag="PLB85_02110"
     CDS             complement(1419..2159)
                     /locus_tag="PLB85_02110"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOU72149.1"
                     /translation="MITHLELPLLSTERINRVYLRISVSAPDLAPRCRPGMFFEIKAG
                     SPSQARRLFKPVSVFEAHDGEIAFLIKVVGPGTQALADLKRGDPLLLTGPLGNSFLEL
                     QGKKVLLVSGGIGYPPLAWLKQVLEPENKVIHIHGGACVTDVFPCDRAYTLDGSFGIQ
                     GLVTRDVREIIKAENIDTVCSCGPMPMLKALQACVSGLLHYASLEAYMACGLGACHGC
                     AVPVGEGWQRVCKEGPVFEASSIRWEEL"
     gene            complement(2162..3430)
                     /locus_tag="PLB85_02115"
     CDS             complement(2162..3430)
                     /locus_tag="PLB85_02115"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOU72150.1"
                     /translation="MTTLIINGTAYINGKFQTRDILIRNGRIARIAKSLPAKADKIVN
                     ASGKHIFPGFVDIHVHLRDPGQTYKEDIVSGTRAAARGGFTSLCAMPNTDPVVDNIAT
                     VDYIQRRAKDLGSARVHVIGAMTKKSEGKEISEMATMQSGGIVAVSDDGNCVQNAKLM
                     LNCIRYATNFGLPLVIHAEDYNLAGKGQIHGGRVASRLGLAGLPGLAEEVIVSRDIML
                     AESTGARLHIAHISTARSIELVREAKERGGKVTCEVTPHHLILTEEACRGFNTNTKMK
                     PPLRTEKDRQACLDALKDGTIDCIATDHAPHADFEKVREFDHAPFGIIGLETAFCALY
                     HHLVKPGHLSLARLAEALTSAPAKILDLPPAELKTGHPADLNIIDLEASTTFTAESML
                     SKSKNTPWLDQTLSGRVVCTFLEGRVTWQE"
     gene            complement(3443..4381)
                     /locus_tag="PLB85_02120"
     CDS             complement(3443..4381)
                     /locus_tag="PLB85_02120"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOU72151.1"
                     /translation="MESKPQFVGRSLFDLEDYSADEIMYILSAAQGMKEINLREYKKI
                     PTLRGKTVCTLFVEDSTRTRMSFELAANRLSADVVSFQASVSSLQKGESLQDTIYTLD
                     AMGIDLYCVRHSSPGSPQLVNKYSGKPVINGGDGRHAHPTQALLDIFSIWEHVGDLKG
                     LKITIVGDILNSRVVRSNLIGMKKLGMEVTVCGPRTLMPGWLEDIFGCRVEYDLREAL
                     SDADVVMGLRMQLERMEEGLFPSLEEYSKHYVLSKDTIQYAKKDALIMHPGPMNRGVE
                     ILPEIADSSHSIIVEQVANGVAVRMALMFLILGGKL"
     gene            4618..5712
                     /locus_tag="PLB85_02125"
     CDS             4618..5712
                     /locus_tag="PLB85_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72152.1"
                     /translation="MKRLMILALAALLFLSACAANREKLSPRATVDLKSADVAYNQQN
                     VEEALALYNKVLDEKEGNPNHAHALRRVADINLYYGETIAERAVEFNKKAFENYNKSI
                     SIMEKYEKPTEKELAAIRDMKKRRTSAWTRMFNAADTQLADGNTASAIEIFETVAAMD
                     PSRTEPLYKLATIYQKDVKDEAKAEAILLKIYEVNPEDNTVQESMGIFYLNKKEYAAA
                     IPFFEKVKQTEPLNVNNLMNLSYCQFELEQYEAAKLNNQMVLNIEPNNEDALADAKYI
                     AFKLEDDAAAVGFLKRLLEIRDDDRDYQDISFLLNKMEDYQEMITYARKWHEYDGMNK
                     DAVRLVILGAQKTSNKALETEFNNILKKMK"
     gene            5772..6686
                     /locus_tag="PLB85_02130"
     CDS             5772..6686
                     /locus_tag="PLB85_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU72153.1"
                     /translation="MKVKIAYLYALGAVLAWSTVSTAFKLSLRHLTPLGLLLFSSVAA
                     TLFLAGTFFGSGRKPRELFNSWWRSLPAGLLNPFIYYLMLFVAYDRLRAQEAQVLNYT
                     WAILLSLLSVWLLKERFRLKDLLALLLSFLGVVVISTRGRIFQLQFDDPLGSLLAVST
                     SLVWASYWILNLKDTRDAKGKLLLNFLVGTLATALYALLRGKMNLPGLFTADGVMLWG
                     LLGALYVGIFEMGFTFILWQKALETSENTASISNLIFLTPFLSLIFIALILKESIHPA
                     TFIGLLVIIGSNFWQKGGSKRKSGLDKG"
     gene            complement(6737..>6999)
                     /locus_tag="PLB85_02135"
     CDS             complement(6737..>6999)
                     /locus_tag="PLB85_02135"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOU72154.1"
                     /translation="LVNRLAFRNIQARNLILKIKYDNFECVTRSCPLPEVSSEKALLF
                     EYAQKLLVTNWDRGRKIRLLGVGVGKLDLGESDGSEQLELPV"
BASE COUNT         1545 a         1914 c         1954 g         1586 t
ORIGIN      
        1 tggggcggtg aaagacaatc agacccgccg aagccactgc tcattgtctt agccgggttt
       61 tatgagctct cgaacccgga gcagccgcac tgtttcgacg cccaatccct ccccggcggc
      121 gcggaagggc tgaacgttag ccgcttatgc ggctaaggag tagttgttgt ttgcagttag
      181 atttatttgc tgcttgttaa cggagcagac agcgtctccg gcatgcatct ctcacaccct
      241 ggttccctgt cgaaaccatg tcacccccta taagcttgta aagaacttaa agacaatata
      301 ttcaaggcgg gattgctgtc aagcggtaaa tcagcactta gcttcgtccg ggcataacct
      361 ggcacattgc cggaagcttt caagattgtt gccggcttgc tggtcctgaa cccctgctgg
      421 tccgtatcaa cagagcctcc cgccttatcc ccgcatttcg tgggagtgtt atacgagtgg
      481 caggacgggg gcgaagctga gagggttaag gctctatgct caaagcgcct tgcctatgat
      541 atcggacagc gagaggtcat tttcccgcag gtgcttttcc agaccgtcgc gtagcttgac
      601 agcggccagg ggatcgctgt aaaaagcggt gccgagggcg attgcggaag cgccggccca
      661 gaaaaattca agggcgtccc gccaactgtg gatgccgccc agggccagaa tggggattgt
      721 taccacctgg gcaaccttgt ggcagagggc cagggcgact ggtttgatcc cgatgccgct
      781 gtagccgcaa accggactgg atatcctgaa cctgccggtt ttccagtcga tcgctgagcc
      841 gtagagtgtg tttatcagcg ccagtgagga agctccgcca gactcggcag cttgggcgat
      901 gcaggctatg tttgttacat tggggctgag tttgaaaatc agttcgcgtt gcgtgatggc
      961 ggacagcctg gaagccaggg aaaaaacaac gtccggatcg gctccgaagg cgattccctc
     1021 tctggcaacg ttggggcagg agacgttcac ctcatagccg gcgattccgt cctgaacctc
     1081 cagtccttca agaatccgca gaaactcgtc ttccgaagaa ccggagaaac tgactatcaa
     1141 cggaatcctg agtaggtcca gcagcgacgg caaatcctca ttcagccagg ctttgaggcc
     1201 aggattctgg aggccgatgg aattgagcag cccggcctcg gtttcataga gacggggagg
     1261 tggattgccc gctttcggag ccagggtgat ggttttggtg gtataggcgc cgaggacatc
     1321 ctggtcgaag agctcgaaat aatcggggtc gaaagtgccg gaagcaactg ttaccgggct
     1381 tttcagccgc aggcgcccca gcgaggtggc aaggcttttc acaactcctc ccagcgaatg
     1441 gaggatgcct caaacacagg cccttccttg cagacacgct gccagccctc ccccaccggg
     1501 actgcgcagc cgtgacaggc gcccagcccg caggccatgt aggcttccag agaggcatag
     1561 tgcagcagac cgctaacgca ggcctgaagc gccttcagca tgggcatggg cccgcaactg
     1621 cagaccgtgt cgatattctc ggctttgatg atttctcgca cgtcgcgtgt gaccaagcct
     1681 tgaattccga aactgccgtc cagggtgtaa gcccggtcac agggaaagac gtccgtcacg
     1741 caggctccgc cgtggatgtg gatgaccttg ttctcaggct ccagaacctg cttcagccag
     1801 gccaggggcg gataaccgat accaccactc acaagcagga cctttttgcc ttgaagttcc
     1861 agaaaagagt tgcccagcgg cccagtcaac agcaacggat cgcccctctt caaatcagcc
     1921 aaagcctggg tgccaggacc tacaaccttg atcaggaaag cgatttcacc atcatgagct
     1981 tcaaacacgc tcaccggctt gaagaggcgc ctggcttggg aaggcgatcc cgctttgatt
     2041 tcaaagaaca tccccggacg gcaacgcgga gccagatcag gcgccgaaac gctgatccgc
     2101 aggtaaaccc ggttgatccg ttcggtgctg agtagcggca gttcaagatg ggtgatcaca
     2161 ctcactcctg ccaggttacg cggccttcca ggaaggtgca gactacacgt ccagagagcg
     2221 tttggtccag ccagggggtg tttttgctct tggagagcat ggattccgcg gtgaaagtgg
     2281 tgctggcttc cagatcgatg atgttgaggt cagcgggatg gccggttttg agttcagccg
     2341 gcggcaagtc caggattttg gctggagcac tggtcaaggc ttcagccaag cgggccaggg
     2401 agagatggcc gggcttcacc agatggtggt agagcgcgca aaaagctgtt tccaggccta
     2461 tgatgccaaa gggggcgtgg tcgaattcgc gcactttttc aaaatcggca tggggagcgt
     2521 gatcggtggc gatgcagtct atggtcccgt ccttaagcgc gtccaaacag gcctgtcggt
     2581 ccttttcagt tcgcagcggg ggtttcatct tggtgttggt attgaagccc cggcaggctt
     2641 cttcggtgag aatgagatgg tgaggagtga cctcgcaggt gacttttcca ccgcgttcct
     2701 tggcttcgcg caccagttca atggaacggg cggtgctgat atgggcgatg tgcagcctgg
     2761 cgccggtgct ttccgccagc atgatgtcac gcgagacgat aacctcctcg gccagaccgg
     2821 gaagaccggc aagccccagc ctggaggcga cacgtccgcc atggatctgg cctttaccag
     2881 ccagattgta gtcctcggca tggataacca gtggcagtcc gaaattggtg gcgtagcgga
     2941 tgcagttgag catcaacttg gcgttctgaa cgcaatttcc atcatccgaa accgcgacaa
     3001 tcccgccgga ctgcatggtg gccatttcgc tgatttcctt gccctcgctc tttttggtca
     3061 tggcaccgat cacgtggacc ctggccgaac ccaggtcctt ggcgcgacgc tggatgtagt
     3121 caaccgtggc gatgttgtca accacgggat cggtgttcgg cattgcgcag aggctggtga
     3181 atccgccccg ggccgcagcg cgggttccgg aaacgatgtc ttccttatag gtttggccgg
     3241 ggtcacgcag atgaacgtgg atgtccacaa aaccgggaaa gatgtgtttt ccggaggcgt
     3301 tgacgatttt gtccgccttg gccggcagac ttttggcgat tcttgcgatc ctgccgttgc
     3361 ggatcaggat gtcacgggtc tgaaactttc cgttgatgta ggcggtgcca ttgatgatga
     3421 gtgtggtcac tttttccctc ccttatagtt ttccgccgag gatgaggaac attagtgcca
     3481 tgcgcacagc cacgccgttg gctacctgtt ccacaattat gctatggctg ctatccgcga
     3541 tttcaggcag gatttccacc ccgcggttca tcggcccggg atgcattatc agggcgtcct
     3601 ttttggcata ctggatggta tctttggaga gcacgtagtg cttgctatat tcctccaaac
     3661 ttggaaacaa gccctcctcc atcctctcca gttgcatgcg caggcccatc acgacgtcgg
     3721 cgtcgctcag agcttcgcgc aggtcatatt ccacacggca tccgaaaatg tcctccagcc
     3781 agccaggcat cagggtccgg gggccgcaga cggtcacctc catacccagt tttttcatgc
     3841 cgatgaggtt ggaacgcacc accctgctgt tcaggatgtc gcccacgatg gtgatcttaa
     3901 gacctttgag atcgcccacg tgttcccaga tggaaaagat gtctagcaag gcctgggtgg
     3961 gatgggcatg gcgtccgtca ccgccgttga tgacgggctt tccggagtat ttgttcacca
     4021 gttgggggct gccgggactg ctgtggcgga cgcagtagag gtctatgccc atggcgtcga
     4081 gggtatagat ggtgtcttgc aggctttcgc ctttctggag tgaggaaacc gaggcctgaa
     4141 agcttacgac gtctgcgctg aggcggttgg cggcgagctc gaaactcatt ctggtacggg
     4201 tgctgtcttc aacaaaaagg gtgcagacgg ttttgccgcg cagggtgggg atttttttgt
     4261 actcgcggag gttaatctct ttcatgcctt gggcggctga gaggatgtac atgatctcat
     4321 cggcggaata gtcttccagg tcgaagaggc tgcggccgac gaattggggc ttagattcca
     4381 ttttggctcc tttccggctc actggcacgg attaaaggtg ttaatttgct cctttttggc
     4441 gcgcttgggc attcggtcaa gttatttttt gccatcccat catggaaatg ctctcaagcg
     4501 ggaatcaccc atcacgcaag tgcatattgg cgaaacgggt tggaaaaagt cttgactgat
     4561 attcccctta ggatttttgg tatcttttaa aggaaaaaac tcacaggaga tataattatg
     4621 aaacgtctca tgatcctggc tctggctgcg ttgctctttc tttcagcctg cgccgcgaac
     4681 agggagaaac tcagcccccg cgccactgtt gacctgaaat ccgccgacgt ggcctacaac
     4741 caacagaatg tggaagaagc cctcgccctt tacaacaaag tgctggatga aaaagaaggc
     4801 aatcccaacc acgcccacgc cctgagaagg gtggcggaca tcaacctcta ctatggcgaa
     4861 accattgccg aaagggcagt ggaattcaat aaaaaggcgt ttgagaacta caacaaatcc
     4921 atctccatca tggaaaagta tgaaaagccc acggagaaag aactggccgc catccgcgac
     4981 atgaaaaaac gccgcaccag cgcctggacg agaatgttca acgccgccga cacccaactg
     5041 gccgatggaa acaccgccag cgccattgaa atctttgaga cagtagccgc aatggaccca
     5101 tcccgcactg agcccctcta caagctggca accatctacc agaaagacgt taaagacgaa
     5161 gccaaagccg aggccatcct gctcaaaatc tatgaggtga atcccgaaga caacacagtg
     5221 caggaatcga tgggaatctt ttacctgaac aaaaaagaat acgccgctgc catacccttc
     5281 ttcgagaaag tgaagcagac cgaaccgttg aacgtgaaca acttgatgaa cctttcctac
     5341 tgccaattcg aactggaaca atatgaggct gccaagctta acaaccagat ggtgctgaac
     5401 atcgagccca acaacgaaga cgccctcgcc gacgccaaat acatcgcctt caaactggaa
     5461 gacgacgctg ccgcggtggg ttttctgaaa aggctattgg agatccgtga cgacgataga
     5521 gactatcagg atatcagctt tctgctcaac aagatggaag attatcaaga aatgatcacc
     5581 tatgcccgta aatggcatga atatgacggc atgaacaaag acgccgtgcg tcttgtgatc
     5641 ctcggcgccc aaaagaccag taacaaggcc ctcgagactg aattcaacaa catccttaaa
     5701 aagatgaaat aagcctttcc cggatggaag taagcctgtc cgcaatccgg gcaggctttt
     5761 tctttgccag aatgaaagtc aaaatcgctt atctttacgc cctgggcgcc gtgctggcct
     5821 ggtccacagt gtccactgcc ttcaaactca gccttcggca tcttactccg ctgggattgc
     5881 tgcttttctc ttccgttgct gccaccctct ttctggcggg aacgtttttt ggctcaggcc
     5941 ggaagccacg ggagctcttc aattcctggt ggcgttcact accggcggga ttgctgaatc
     6001 ccttcatcta ttatctgatg ctgttcgtgg cttatgatcg cctccgtgcc caggaagcgc
     6061 aggtgctcaa ttacacctgg gcgatattgc tttccctgct ttcggtttgg ctgctcaaag
     6121 agcggttccg cctcaaagac ctgctggcgc tgctgttaag ttttctggga gtggtggtca
     6181 tttccacccg gggacggatt ttccagcttc agttcgatga tcccctggga tctttactgg
     6241 cggtttccac ttcgctggtc tgggcctctt actggattct gaacctaaag gacacccgcg
     6301 acgccaaagg aaagctgctg ctaaatttcc tggtgggcac cctggccaca gcgctctacg
     6361 ccctgctccg cggaaaaatg aacctgcccg ggctcttcac tgcggacggc gtaatgcttt
     6421 ggggtttgct gggcgcgctg tatgtgggca tttttgagat ggggttcacc ttcatcctct
     6481 ggcagaaagc gcttgaaacc agcgaaaaca cggcctccat ctccaacctg atcttcctga
     6541 cccccttcct gtcgctgatc ttcatcgccc ttatcctgaa agaaagcatc catcccgcca
     6601 ccttcatcgg cttgttggtg ataatcggca gcaatttctg gcagaaaggc ggttccaagc
     6661 gaaaatccgg actggataaa ggctgaaacc tgtttcctac gaaccatgcc actgcctccc
     6721 tggattggaa tcagggttaa accggcagtt cgagctgctc agagccgtcc gattcgccca
     6781 agtccagttt gcccaccccc actcccagga ggcggatttt gcggcctcta tcccagtttg
     6841 tcaccagcag tttctgggcg tattcgaaaa gcagagcttt ttcggaactc acttcgggca
     6901 gcgggcagct gcgggtgacg cactcgaagt tatcgtattt tatcttgagg atcaggtttc
     6961 gggcctgaat gttgcgaaaa gccagacggt taaccaatc
//
LOCUS       DAOOWR010000048         6976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_10877_length_6976_cov_3.821413, whole genome shotgun sequence.
ACCESSION   DAOOWR010000048 DAOOWR010000000
VERSION     DAOOWR010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10877_length_6976_cov_3.821413"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /gene="lepA"
                     /locus_tag="PLB85_02140"
     CDS             complement(<1..1616)
                     /gene="lepA"
                     /locus_tag="PLB85_02140"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU72155.1"
                     /translation="MKQEYIRNFCIIAHIDHGKSTLADRFLESTNVVGKVDVAQLLDS
                     MDLEREKGITIKSHAVRMVHSYKGRDYVLNLIDTPGHVDFSYEVSRALASCEGAILLV
                     DASQGIEAQTMSNLYLALDNDLEILPALNKIDLPKADIEGTEHDLCEIMGCLPEDILR
                     VSAKSGTGVTELLDAVCERLPAPTGDPDAPPKALIFDSYFDMYRGVVVLVRLFDGSLS
                     KGDKIKLLATEREYEIEEIGHLGLKFSPQQQLNTGEAGYIIANIKEVADARVGDTITL
                     AKGGCEESLPGFMEPKPMVYSGIFPINGEDYENLVESIAKLKLNDASLIYEKENSSAL
                     GYGFRCGFLGMLHLEIVKERLLREYNIPIIATTPSVRFLITLKNGKEIEVNNPIDFPD
                     PTFIESIEEPFMDTEIIVPTDFIGNIMKLAQERRGIQKNIQYIDEKRVALHYEMPLIE
                     IIFDFYDKLKTVSRGYASLDYSFKEFRPSRVVKVDILINGEKVDAMSFICHQDKAHNW
                     GKSVTETLREVIPRHLFKIALQAAIGGNIIA"
     gene            complement(1834..4932)
                     /locus_tag="PLB85_02145"
     CDS             complement(1834..4932)
                     /locus_tag="PLB85_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU72156.1"
                     /translation="MAQVEYTEYEPRFAKAVAAMWNRSSEGWNGRIWNSSEARVLQKE
                     RDSAYLNLWLALAGEEVIGYVKLSKYSLEEGVAYVELISVDPAWHGKGIGKALMRKCV
                     ERSAELGYQRLDLFTWPGNTKAVPLYKKCGFFWERMESQVTHLMNFLPGILNSPFFRL
                     HFEHFHWYDDLKRELEVTPDGREERGFDYYDYLWEKEGCKLTVSFEKTGRGIAAFATN
                     EIEVECLVDEARPVFGSEHRVSYRFTNRSGKPIPVRIEGLNDANVAFSASHGFQLGEQ
                     AVWEAGFSLSAPQPGDGEWQTQPGVRALLSLDGQNVEMKTGLKTQYPLSLSFLKETML
                     FIPNREYKLQLNVQNHFETDAEFEIEFLPQEPVTLLENRHQIILAPQARSSIPLSISL
                     SRGCLWTPMARVTARPESGGEVTFEKRCEAILRTFEARDQKTLEHYHALINGPYALIV
                     QTRGDKNRASFSKVFGDYCYLSEPMLGEPFSEEFEHLDPFDAVFKDLGGANQLTLSYR
                     SQDFPGVEFALIYRLYPSGMLDLRTRVISLPENAKTTLRLRLMPYSSLITYEHEGELH
                     SLERDQIEAELEWLPETAVTGNFIFCGQEGDTTGIVWDPALKVKLKSWHLAWDFDLDA
                     MLQAGTPESKPIQVFLNQFQNAFQLLDYTRGVYVPVDAAHPSLELRVNQGNPVVSGPV
                     NAELIWRQDRDLTGSFQLSAQGGKSGEPQLLEPGSGQRGMNWKDVELPDAPLVELCCD
                     ASLPLYRLLRRQTLLQPDGGINFRKEGTWLSVDNGFLKLGAAIDSTLPVLLSLETGES
                     EWLDQAWPQFYAKSFYNPYPGGFHVRPGGISLAELSKEKHSLGIAEVKDQHGNSWRGL
                     AFDTRIDNYKLFRGMRFRQYYLTLPGLPLLVATTEVVSGTGFACYESFPLMGFFAPGG
                     KLENCRIHVPIDQERWQSVEAGREDLRYWDYSRQFSVENKESGWQMHFPNPAKVETGI
                     HIDKAVARLRIGRFSYRATEYPKWLNPIFILFSRERPAWESLGQLLDLRFGTGYRDIQ
                     PL"
     gene            complement(5039..5566)
                     /locus_tag="PLB85_02150"
     CDS             complement(5039..5566)
                     /locus_tag="PLB85_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOU72157.1"
                     /translation="MLEREKPQYPEVKFCQGCGGPLVPKEDHEGKTRLVCEDCGRVHY
                     KNPIPAVAVLVLNPAGELLLVKRKFAPSAGMWALPSGYMEIFLSPEENAVAELKEETG
                     LEGEVEHFISWFYGYSPIYERVLSLGFRMRVTGGSLQAGDDAEEAEFFPLQELPPIAF
                     DAHRSFIRLETGLEI"
     gene            6571..>6976
                     /locus_tag="PLB85_02155"
     CDS             6571..>6976
                     /locus_tag="PLB85_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72158.1"
                     /translation="MKKLLLSGLLLLLVVFAFADAYTIGDGTSTQNYIPFYGFYNYNW
                     SKVIYTQAEINTAGLTNGENIIGIGFYVGNTPSNYTMLDQRVFVRHTDATAYETTDVG
                     YPDNTQFQQVFQGDLTFDGGGWHYIVFSAPFAW"
BASE COUNT         1495 a         1928 c         1872 g         1681 t
ORIGIN      
        1 gcgatgatgt tgcctccaat ggctgcttgc agggcgatct tgaagagatg gcgcgggatc
       61 acctcacgca gagtttcggt tacgcttttc ccccagttgt gggctttgtc ctggtggcag
      121 atgaagctca tggcgtccac tttttcgccg ttgatgagga tgtccacttt caccactcgg
      181 gaggggcgga attccttgaa ggagtaatcc agcgaggcgt agccgcggct tacggttttc
      241 agcttgtcgt aaaaatcgaa gatgatctcg atcagtggca tctcatagtg gagggccacg
      301 cgcttttcat cgatatattg gatgtttttc tggatgcccc ggcgttcctg agccagcttc
      361 atgatgttgc cgatgaagtc tgttggaacg atgatctcgg tgtccatgaa aggttcctcg
      421 atgctttcga tgaaagtggg atcgggaaaa tcgatgggat tgtttacctc gatctctttg
      481 ccgtttttga gggtgatgag gaagcgcacg ctcggcgtgg tcgcaatgat ggggatattg
      541 tattcacgca ggaggcgttc tttcacgatc tcgaggtgca gcatgcccag aaagccgcag
      601 cggaagccgt atccgagggc gctggagttt tccttttcat agatcaggga ggcgtcgttg
      661 agcttgagct tggcaatgga ttccaccagg ttttcatagt cctcgccgtt gatggggaaa
      721 atgccggaat aaaccatggg cttgggttcc atgaaacccg gcaaactctc ttcgcagccg
      781 cctttggcca gggtgatggt atcgccgaca cgagcgtccg ccacctcttt gatgttggcg
      841 atgatgtagc ctgcctcgcc ggtgttcaat tgctgctggg gggagaattt caggcccaag
      901 tggccgatct cctcgatctc atactcccgc tctgtggcca ggagcttgat cttgtcgcct
      961 ttgctcagcg agccgtcgaa aagccgcacc agcacaacca cgccgcggta catatcgaaa
     1021 tacgaatcga atatcaaagc tttgggcggg gcgtccggat cgcctgttgg tgcgggaagc
     1081 ctttcacaga cagcatccag cagctcggtg accccggtgc cggatttggc gcttacccgc
     1141 aaaatgtctt ccggcaggca gcccatgatt tcgcagaggt cgtgttccgt gccttcgatg
     1201 tcagccttgg gcaggtcgat cttgttcagc gcgggcagga tttccaaatc gttgtctagc
     1261 gccagataga ggttgctcat cgtctgggcc tcgatgccct gggaggcgtc caccagcagg
     1321 atggcgcctt cgcaggaggc cagcgcgcgc gaaacctcgt aggagaaatc cacgtgtccg
     1381 ggtgtgtcga tgaggttcaa aacatagtcc cggcccttgt aactgtggac catccgcacc
     1441 gcgtggcttt tgatggtgat gcctttttcg cgctcgaggt ccatactatc gagcaactgt
     1501 gccacatcca ctttgcccac cacgttagtg ctttccagaa agcggtccgc cagggtagat
     1561 ttcccatggt cgatgtgcgc tatgatgcaa aagttgcgta tatattcctg tttcatgttc
     1621 atcctaagct gtttcacaac caccttgttt tcagggaatt gtgccaaggc ggcatattct
     1681 tacccagaat tggcggtgtt cacttttgtc aacaatttaa tgcccagccc acatccatcc
     1741 gttttttcag atggggtgtt atcaaatggt tttcaccgcc cacaaccctg tggattggtt
     1801 gatcttacgc cagggatggc agtcgtcacc ttactacagg ggctggatgt ccctgtatcc
     1861 agtgccaaag cgaaggtcca gaagttggcc caggctttcc caggctggcc gttcccggct
     1921 gaaaaggatg aagatcgggt tcaaccactt gggatattct gtcgccctgt agctgaaacg
     1981 accaattcgt aagcgggcca ctgccttgtc gatgtggatt cctgtttcca ccttggcggg
     2041 gttggggaaa tgcatttgcc agccggattc cttgttctcc acgctgaact gccgggaata
     2101 atcccaatac cgcaaatctt ccctccccgc ctccacgctc tgccagcgtt cctggtcgat
     2161 gggaacatgt atccggcaat tttccagctt gccgccgggt gcgaaaaagc ccatcagcgg
     2221 aaaactctca taacaggcaa aacccgttcc ggaaaccact tccgtggtgg cgacgagcaa
     2281 aggcagcccc ggcagcgtga ggtaatattg ccggaaccgc atccccctga acagtttgta
     2341 gttatctatc ctggtatcaa acgccaggcc ccgccagcta ttgccgtgct gatccttcac
     2401 ctcggcgatg ccgaggctgt gtttttcctt gctgagttca gccagagaaa ttccgcccgg
     2461 ccgcacgtga aagcctccgg gatagggatt atagaaggat ttggcataaa actgcggcca
     2521 ggcttggtcc agccactcag attccccggt ttccagagaa agcagcaccg gtaaggttga
     2581 atcgatggct gcgcccagtt tcaggaatcc gttgtccaca ctcagccagg ttccttcttt
     2641 acggaaattg atccctccgt ccggctgcag gagagtttgc ctcctgagaa gacgatacag
     2701 cggaaggctg gcatcacaac acaactccac cagaggcgcg tccggaagtt ccacatcctt
     2761 ccagttcata ccccgctggc cggaaccggg ctccagcagt tgcggctctc cggattttcc
     2821 tccctgcgcg ctaagttgaa acgagccggt cagatcgcgg tcctggcgcc aaatcaattc
     2881 cgcatttacg gggccggaaa caactgggtt tccctggttg acccgcagtt ccagactggg
     2941 atgtgccgcg tcaaccggga catagactcc ccgggtgtaa tcgagcagtt gaaaggcgtt
     3001 ctggaactgg ttcagaaaga cctggatggg tttactttcc ggtgtgccag cctggagcat
     3061 ggcatcgaga tcaaagtccc aggctagatg ccaacttttc aatttcactt ttagggctgg
     3121 gtcccaaacg atgcccgtgg tgtcaccttc ctggccgcaa aagatgaaat tgccggtcac
     3181 ggcggtttca ggcagccatt ccagttcagc ttctatctgg tcacgctcca gactgtgcaa
     3241 ttcaccctcg tgttcgtagg tgatcaagct gctgtaaggc atcaaccgca ggcgcagagt
     3301 ggttttggcg ttttccggaa ggctgatgac ccgggtccgc aaatccagca tcccagaggg
     3361 atagagcctg taaatgaggg cgaattccac gccgggaaaa tcctggctgc gatagcttag
     3421 tgtgagctgg ttcgcgccgc cgaggtcctt gaaaaccgcg tcaaaggggt caagatgctc
     3481 gaactcttcg gaaaaaggct cgcccaacat gggttcggaa agatagcaat aatcgccgaa
     3541 aaccttggag aagctagcgc ggttcttatc ccctctggtc tgcacaatga gggcatacgg
     3601 accgttgatg agggcgtggt aatgctccag ggttttctgg tcacgcgcct caaaggtcct
     3661 cagaattgcc tcgcagcgtt tttcaaaggt cacttcgcct ccggattccg gcctggcggt
     3721 cactcgcgcc attggggtcc agaggcagcc gcgggacagg ctgatgctta ggggaatgct
     3781 tgacctggcc tggggcgcga ggatgatctg atgcctgttc tcaagcaggg tcacaggctc
     3841 ctggggcagg aactctattt caaactcggc atcagtttca aaatggttct gaacattgag
     3901 ctggagtttg tattcccggt tgggaatgaa caacatggtt tcctttaaga aggaaaggga
     3961 gagcggatac tgggttttaa gcccggtttt catttccacg ttttgtccat ccaggctcag
     4021 cagcgcccga acacccggct gggtctgcca ttcgccgtcg ccaggctggg gcgccgaaag
     4081 gctgaatccc gcttcccaga cggcctgttc cccaagctgg aaaccgtggc tggcgctgaa
     4141 ggccacattg gcgtcgttca agccctcgat ccgcaccgga attggcttgc cggagcggtt
     4201 ggtgaatctg tagctgaccc tgtgttcact cccgaaaacg ggccgggctt catccacaag
     4261 gcattccacc tcgatctcgt ttgtggcaaa ggcggcgatg ccccgccctg ttttttcaaa
     4321 gctgacggta agcttgcacc cctctttttc ccacaggtaa tcatagtaat caaagccccg
     4381 ttcctccctg ccgtctggcg tcacttcaag ctcccgcttc agatcatcat accagtggaa
     4441 atgttcgaaa tgaagccgga aaaacgggct gttcagaatc ccaggcagaa agttcatcag
     4501 atgggtgacc tggctttcca tcctttccca gaagaagccg cattttttgt agagcggaac
     4561 ggccttggtg ttgcccggcc aggtgaatag gtcgaggcgc tgatatccca gttccgcgga
     4621 acgttccacg cattttctca tcagggcttt gccgattccc ttgccgtgcc aggctgggtc
     4681 caccgaaatc agctccacat aagccacacc ctcttccaaa gagtatttgg agagcttgac
     4741 atagccgata acctcctcgc cggccagcgc cagccaaagg ttcagatagg ccgaatcacg
     4801 ctccttttgc agaactctgg cctcgctgct gttccaaatc cggccgttcc agccttcgct
     4861 gctgcggttc cacattgcgg caacggcttt ggcaaagcgg ggttcgtatt cggtgtattc
     4921 cacttgggcc atttttcctc caatccggat ggatttaatc cctaatcgcg cctcaaagga
     4981 ttatgtcaag caaaagctgg aacgcgggca ttcagccgat tcccggttca taaggcaatc
     5041 agatttccag acctgtctcc agcctgatga agctgcggtg ggcgtcaaag gcgatgggcg
     5101 gaagttcttg caggggaaag aactcggcct cctcggcgtc atcaccggcc tgcaggcttc
     5161 cacccgtcac ccgcatccgg aaaccgaggc tcagaacgcg ttcgtagatg gggctgtagc
     5221 catagaacca cgatatgaag tgctcgacct ctccctccag cccggtttct tccttcagtt
     5281 cagccacggc gttctcctcc ggggataaaa atatctccat gtaaccgctg ggaagcgccc
     5341 acattcccgc gctgggagcg aatttacgct tcaccaaaag gagttccccc gccgggttga
     5401 gcaccaaaac ggccactgcg gggatgggat tcttgtaatg cacccggccg caatcttcgc
     5461 aaaccaggcg ggttttgcct tcgtgatcct cttttggaac aagcgggcct ccgcagccct
     5521 ggcagaactt cacttcgggg tattggggtt tttcacgttc aagcataatg ccattatgtc
     5581 catccgctcc agcctgtcaa cccctttgtg cacggaatcc gcgtaacgct ccaaagcctg
     5641 gcgggttacc ggttcatggc tttctggttg ggcatccgcg gccacttgat ctaaaagaga
     5701 gccatacctg ccaacccgcc gcaaggtcct gtctaccgca cccttaacgc ccgtttacat
     5761 ccccctccca tgcctcccgt ataatacccg catcaagtgc ggccattgtg cgggaggcgc
     5821 atccgggaga cgggaaaggg ccttaacggc cagaggatat cgcttcgttt attcgcggag
     5881 agcctggcgc tgggatgacg gcagtagcgc ccgactcatt tgaacaccga ccctgtattt
     5941 cgcaaggcca tgctggtcga acaacaggtc caactccttg caaggaaatg ctttgacggt
     6001 attcgtgaca tattccggat cagaaatggt tcacggtatt gatgccgtaa tgatggagaa
     6061 aacagggact atggcataaa aatagcttta caaaaaaatg gatgcgtatt tatttggttc
     6121 gatgtgttaa ttgtgagttt tgatatattg ttggcatcct tcgcgctggt ttttccggcg
     6181 aggttgccgt aataagtaag ctgatcaacc ataacaccaa gacaaaggta aaaaggagat
     6241 gatcgaatga aaagttgtgt cgtcttgccg tttcggctgg cggtttttac ccttgggagc
     6301 gttcagtgct gattcgcagt gttgtgttcg caagatcaga gccgcgtcct gaacggctgt
     6361 tcccgtcatt tggagttctc tccaatacag gggcttgtca cgcttttcat tatccgcaaa
     6421 atcttttcca aacaaggaat cttcaacaaa ccaaagctat cactttaatt caggtttcca
     6481 atccaacatt cgggcaaggt tcaaactatc ggtcccgaat cattactacc ctgtcaaccc
     6541 gtttaacagt atctacaaca aggagatgag atgaaaaaac tgcttctctc aggcttgctc
     6601 ctgcttctgg tggtgtttgc ctttgccgat gcctacacca tcggcgacgg aaccagcacc
     6661 cagaactata ttccgttcta tggattttac aactacaact ggagcaaggt tatttacacc
     6721 caggcagaaa tcaacactgc cggactaacc aacggggaaa acatcatcgg cattggtttc
     6781 tacgttggca acacgcccag caactacacc atgctggacc agcgtgtgtt cgttcggcat
     6841 accgatgcca ctgcctatga gacgactgat gtcgggtacc ctgataacac acagtttcag
     6901 caggtgttcc agggcgacct gaccttcgac ggcggaggct ggcactacat cgtgttcagc
     6961 gcccccttcg cctgga
//
LOCUS       DAOOWR010000049         6903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11041_length_6903_cov_3.339077, whole genome shotgun sequence.
ACCESSION   DAOOWR010000049 DAOOWR010000000
VERSION     DAOOWR010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11041_length_6903_cov_3.339077"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /gene="malQ"
                     /locus_tag="PLB85_02160"
     CDS             complement(<1..1281)
                     /gene="malQ"
                     /locus_tag="PLB85_02160"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOU72159.1"
                     /translation="MKNFGVLLHISSLPSEYGIGDLGPGAYQFADILVERGIKYWQIL
                     PLYQTGYGNSPYNPLSAFALNPYLISPDLLYEDGLIDLPDLEAAKLPVTDHVPYNAVY
                     RLKDALIAKAADNYLTDSVISDFIESDALYMKPYLAYITLSNLYGDDNWQLFRPEHRQ
                     YTPELYDSLFKSYGRQMLCAACAQLIVKDQLTRLKEYINGQGLQLIGDMPLYLSYQSS
                     EVWANQHLFELDAAGNRLRVAGVPPDAFSETGQLWGNPLYRWDVMREDGFQLFTHRIR
                     DALAYLDKLRLDHFIGYVNYWAVAPKSVGADGQPELPETALEGSWEKALPEDFFPLLQ
                     SLFPMERFIAEDLGILNGEVCAYRDKYGLPGMIVLQFCFEESVPWVRDFPPERFIYTG
                     THDNPTIREWFDSLPGDSPSRANLFQFISENASLF"
     gene            complement(1278..2276)
                     /gene="holA"
                     /locus_tag="PLB85_02165"
     CDS             complement(1278..2276)
                     /gene="holA"
                     /locus_tag="PLB85_02165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU72160.1"
                     /translation="MPLDALEFDKVRLSLGDNFLLTGGDAFLMDTVTDRVRLELKQRD
                     NVDLVIVYGDEVNSAQINDLLDTYSIFSSTKLILFRNADMMKKAELDCLADYFTNPSP
                     QQSLVITAEKTDARLSGWKKIRQACQIVACDPPRYSGMFAAWLHKALARMGKSMSDSA
                     RSAFTERVELDYSSANNELQKLSLLVGDRKVITEADVMRAIGSSRVGTQIAFAKALGN
                     RQLKEALELLDRLLASDQKALQITSQINRTFLTIYHILLMKEAHLSPSEISGKHLMDI
                     FADQRKHYMGFVQKYTLPQLEKILAILLDTDSKLKSTHASDAVLLTTCILKIIDTV"
     gene            complement(2276..3091)
                     /locus_tag="PLB85_02170"
     CDS             complement(2276..3091)
                     /locus_tag="PLB85_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOU72161.1"
                     /translation="MSFISAVLLGILQGLTEFLPVSSSGHLVLAQHFFGVESSGDTLF
                     EVFMHLGTLLAVLVFFRRRIQVLLVSLFSWKRTLRHEAHRRNRNVILYLVCASLATGL
                     VYLLFGDLFEALYSRPLVVAFMLLVTGIVIFVSDRLRSGDIPASNMGVPRGIFIGLIQ
                     GIAIIPGISRSGATIAGSLLAGLKRRDAAEFSFLLSIPAILAANLVNLDLFKQLSWKV
                     FAAYFGGFVAAFGVAYLVIGFMLELISRAKLKYFAYYCWAVGLLCVALLIWKP"
     gene            complement(3099..4256)
                     /locus_tag="PLB85_02175"
     CDS             complement(3099..4256)
                     /locus_tag="PLB85_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOU72162.1"
                     /translation="MAKRTKKRVAPKTKARDRSLWLYLLILLLCAGIVWMITLPDDAI
                     EEPVKEPVARVAEFDLNKVIASLETKFSLPEGGISRKKSRGVEQLTLPVDRGKMDLSY
                     ANFIVKGVFETHGANLSSGRVEGGKQVLSFSRGEDEFLVNLVYAAPAARKASSPKYIA
                     IVVDDFGSVSGEMLQQWLELPTEVCFAIISRLKHSEETMNLANAQGRETLVHVPMEPI
                     DYPRQNPGQDPILVQMDQARVEKTLLRHINSLPLCMGINNHMGSLATTDSDIMGWVMG
                     VLKKKGKSFLDSRTSTVSIAYQEAQKARVPAYRNDLFLDSPNISDANLETRLGLVQEL
                     GQRKNTVVVISHCHNAEKLEYLKKFISRVKTLGFTLILLSRVGKTDVPQIL"
     gene            complement(4240..5085)
                     /locus_tag="PLB85_02180"
     CDS             complement(4240..5085)
                     /locus_tag="PLB85_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72163.1"
                     /translation="MHKILIRIFSLLFGDFNGVQIFIYPMTLFYWIDTESFLWASTGL
                     FSSQRYFFPLVVFLLAFIFMAFLVMKNHCALYSDVWPHPPAEKRSFNLTVIGLHKLLL
                     IILFAVGMIYLVSAFLNYFYGIALPLRVIYLMLARIVCVLMILGYAIRNSWTRPHREK
                     GLDITRASALVRKDFRERRGRYLLHAVVYALLAFIFSAFYNLIVLNVFYLIFSIVGVS
                     PNLYLAVTSGFPALFYDIMVICVALMLSNLLFSPLVKLGVDLSERLHPLRNQPMEEAP
                     DGEAN"
     gene            complement(5191..6201)
                     /gene="tsaD"
                     /locus_tag="PLB85_02185"
     CDS             complement(5191..6201)
                     /gene="tsaD"
                     /locus_tag="PLB85_02185"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU72164.1"
                     /translation="MNPILAFESSCDDTSVAIVDDGYEVICNLVSTQADHADFGGVLP
                     ELASRLHLQNILHLTEAALRKSGLKKEDISALAVSINPGLIGSLLVGLSFAKSLAWSW
                     QKPLITVNHMLSHIFANFIEHPWLQPPFLALVVSGGHTELVHFSTLTDFKVVGKTLDD
                     AAGETFDKTAKLLGLGFPGGPELDKAAQGGDPAFHHFPRALKNRDDLNFSYSGLKTAV
                     LEYLARKDRDFIDAHLHDLAASIQQAIIDPLIGKTLKYARQQGLETILLAGGVAANSA
                     LRKQIREAAEAQGACVYIPAQQFCVDNAAMVGAAALPKLKEGKFSSLDVNAFSAKGTK
                     TL"
     gene            complement(6198..>6903)
                     /locus_tag="PLB85_02190"
     CDS             complement(6198..>6903)
                     /locus_tag="PLB85_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72165.1"
                     /translation="MMKNNLGLKIVALVMAVFFWLQITLLSQHESKTSLPLKLVNATG
                     EDSLRQPPRRIATSVYGRGLDILRLRYSQAHIQMNAADFWAGNAADYLALNVPEKLNV
                     KVLGVMPPTLAEHLSEAGQARAENAHSAAKNGKKAPSAAGTEPSQSDSEEGQLQTKIL
                     TDLVITPPQGVKTFPPQATLKVKGNASLLAGLPSGVRVFAASQPDAGGQYKLRYEVPE
                     GITVLDITPKQVRASR"
BASE COUNT         1579 a         1777 c         2051 g         1496 t
ORIGIN      
        1 gaaaagcgaa gcgttttcac tgatgaattg gaagaggttg gcccggctgg gggaatcccc
       61 gggcaggctg tcaaaccatt cgcggatggt gggattgtcg tgagtgccgg tatagataaa
      121 gcgttcgggc ggaaaatcgc gcacccaggg cacgctttcc tcaaagcaaa actggagcac
      181 gatcatgccg gggaggccat atttgtcgcg ataggcgcaa acctcgccgt tgaggatgcc
      241 caggtcctcc gcgatgaagc gctccatggg aaaaagcgac tggagcagag ggaagaaatc
      301 ctccggcagc gccttttccc aactgccttc cagggctgtt tccggcagct caggctgccc
      361 gtcggcacca actgacttgg gggcaacggc ccagtagttc acatagccga tgaagtggtc
      421 gaggcggagc ttgtccagat aggcaagcgc gtcgcggatg cggtgggtga aaagctgaaa
      481 tccgtcctca cgcatcacgt cccagcggta aagagggttt ccccaaagct ggccggtttc
      541 gctgaaggcg tccggcggca ccccggcgac acgaagacgg ttaccagcgg cgtccagttc
      601 gaacaaatgc tggttcgccc aaacttccga gctttgatag gaaaggtaaa gcggcatgtc
      661 gccaatcagt tgaaggccct ggccgttgat gtattccttg aggcgggtta gctggtcttt
      721 cacaatcaac tgggcgcagg cggcgcacag catctggcgt ccgtagctct tgaacaggct
      781 gtcgtaaagc tccggagtgt attgccggtg ttcagggcgg aagagttgcc agttgtcgtc
      841 tccatagaga ttgctcagtg tgatataggc aagatagggc ttcatataca aagcatcgct
      901 ttcaataaag tcagatatca cgctgtctgt aagatagtta tccgcggctt tggcgatgag
      961 ggcgtcttta aggcgataaa ccgcgttgta gggaacgtgg tcggtcacgg gcaatttggc
     1021 ggcttcgagg tcgggcaggt ctatcaggcc gtcctcatag aggaggtcag ggcttatcag
     1081 atagggattg agggcgaaag cggagagggg attgtaaggc gaattgccgt atccggtttg
     1141 atagaggggc aggatttgcc agtatttgat gccccgctcc accaggatgt cggcaaactg
     1201 ataggcccca gggccaaggt cgccgatgcc gtattccgag ggcagggaac ttatatgcag
     1261 caatacgccg aagtttttca taccgtatca atgattttaa ggatgcaggt ggtgagcagc
     1321 acggcgtcgc tggcgtgggt ggatttgagc ttggaatctg tatcgagcag gatggcgagg
     1381 attttttcca gttggggaag cgtgtatttc tgcacgaaac ccatgtagtg cttgcgttgg
     1441 tcggcaaaaa tatccataag gtgctttccg ctgatttcgg aggggctgag atgggcctcc
     1501 ttcatcagca ggatgtggta gatagtaaga aaggtccggt ttatctggct ggtgatctgc
     1561 agggcttttt ggtcggaagc caacaggcgg tccagcagct ccaaagcttc cttgagctgt
     1621 ctattgccga gggctttggc aaaggcgatc tgggttccca cccgggatga accgatggcc
     1681 cgcatcacat cagcttccgt gattactttg cggtcgccca ccagcaggga cagcttttgc
     1741 agctcgttgt tggcgctgga ataatccagc tccacccttt ccgtgaaagc ggagcgggcg
     1801 gaatcggaca tggatttgcc catgcgtgcc aaagccttgt gcagccaggc ggcgaacata
     1861 ccgctgtagc ggggaggatc gcaggcgacg atctggcagg cctgcctgat ctttttccag
     1921 ccggaaaggc gggcgtctgt cttttcggcg gtgataacca ggctctgctg cggggaggga
     1981 ttcgtgaaat aatccgccag gcaatccaat tcagcctttt tcatcatatc cgcgttgcga
     2041 aagagaatca gcttggtgct ggaaaagatg gagtaggtat ccagcaaatc gttgatttgg
     2101 gcgctgttaa cctcgtcccc gtaaacaatc accaggtcca cgttgtctct ttgcttcagt
     2161 tcaaggcgca ccctgtcggt taccgtgtcc atcaggaacg cgtctccgcc ggtgaggagg
     2221 aagttgtctc ccaggctcag tctgaccttg tcaaattcca gggcgtccag gggcatcagg
     2281 gtttccagat gagcagcgcg acgcagagca agccgaccgc ccagcaataa taggcgaaat
     2341 attttaactt ggctctggag atcagttcca gcatgaagcc tatcaccaga taagccaccc
     2401 cgaaagcggc cacaaaacct ccgaagtaag cggcaaacac tttccaggaa agctgtttga
     2461 agaggtcgag attgacaaga ttggcagcca ggatggccgg aatgcttagc aggaaggaaa
     2521 attccgccgc gtcacggcgc ttgaggccgg ccagcaggga acccgcgatg gtggcgccgg
     2581 aacgcgaaat ccctggaatg atggcaattc cctggatcag accgatgaag attccgcgcg
     2641 gaacgcccat gttcgaagcg gggatgtcgc cagaacggag gcggtcggaa acaaagatga
     2701 caattccggt gaccagcagc atgaacgcca ccaccagggg ccgggaatac agcgcttcaa
     2761 acaggtcccc gaaaagcaga tagacaagtc cggtggcaag agaggcgcaa acaaggtata
     2821 aaatcacatt gcggttgcgc cggtgggctt cgtgccgcaa tgtccttttc caactgaaaa
     2881 gtgagaccag caggacctgg atgcggcggc ggaaaaagac cagaacggcc agcaaagtgc
     2941 ccagatgcat gaaaacctca aacagggtgt caccgctgga ctcgacccca aagaagtgct
     3001 gggccagcac cagatggccg gaactgctta ccggcaaaaa ctccgttaaa ccctgtaaga
     3061 tgccaagcag gaccgcgctg atgaaactca cattatcctc acaaaatctg gggcacgtcg
     3121 gttttgccca ccctggagag caggatcagg gtaaagccca atgtcttgac ccggctgatg
     3181 aactttttca ggtattccag cttttcagcg ttgtggcaat gcgaaatcac tacaacggtg
     3241 tttttgcgct ggccaagttc ctgaaccagg cccagcctcg tttccaggtt ggcgtcggag
     3301 atgttgggcg aatccaggaa gaggtcgttg cggtaagccg gcacgcgtgc tttctgggct
     3361 tcctgatagg cgatggagac ggtggaggtg cggctgtcga ggaaggattt gcctttcttt
     3421 ttaaggactc ccatcaccca gcccataatg tcgctgtctg ttgtcgctag gctgcccatg
     3481 tggttgttta tccccatgca gagcgggaga gaattgatgt ggcgcagcag ggttttttcc
     3541 acccgggcct ggtccatttg caccaggatg ggatcctggc cgggattctg gcgcggatag
     3601 tcaattggct ccatgggcac gtggaccagc gtttcacgcc cctgggcgtt ggcgaggttc
     3661 atggtttcct cgctgtgctt gaggcgggaa atgatggcaa aacacacctc agtgggcaat
     3721 tccagccatt gctgaagcat ttcgcccgac accgagccaa agtcatccac cactatggcg
     3781 atgtattttg gggaggatgc tttcctggca gcgggagcgg cataaaccag attgaccaag
     3841 aattcatcct cacccctgga aaagctgagg acctgcttgc cgccttcaac gcgtccggag
     3901 ctgagatttg ccccatgtgt ttcaaacacg cctttcacga tgaaattagc atagctgagg
     3961 tccattttcc cacggtccac gggaagggtg agttgctcca ccccacggga tttcttgcgg
     4021 cttatcccac cctcgggcag agaaaacttg gtttccaggg acgcaataac cttgttaagg
     4081 tcgaattccg cgactcgagc gacaggttcc ttcaccggtt cttcgatcgc gtcatccggc
     4141 aaggttatca tccatactat ccccgcgcag agcagaagaa tcagcagata gagccaaagc
     4201 gacctatccc gggccttggt tttgggcgca acgcgtttct tagttcgctt cgccatcggg
     4261 ggcctcctcc attggctgat tgcggagggg atgcagcctt tcggagaggt ccacccccag
     4321 cttcaccagc ggggaaaaaa gcaggttgga aagcatcagc gccacacaaa tcaccataat
     4381 gtcatagaac aatgccggga aaccgctggt gacagcgaga tagaggttgg gcgaaacacc
     4441 gacgatgctg aaaatcagat agaacacatt caacacgata aggttgtaaa atgccgaaaa
     4501 gataaaggcc agcagcgcat aaaccactgc gtgaagcaga tagcggcccc ggcgctctcg
     4561 aaaatccttg cggaccagag ctgatgccct cgtgatatcc agcccctttt cgcggtgggg
     4621 gcgtgtccag ctattgcgga tggcgtagcc gaggatcata agcacgcaga cgatcctggc
     4681 cagcatcagg taaataaccc taagggggag cgcaatccca tagaaataat tcagaaatgc
     4741 tgacaccaga tagatcatcc caacggcaaa aagaatgatc agcagcagtt tgtgcaaacc
     4801 gatcaccgtg aggttgaaag accttttctc cgctggagga tgcggccaga catcgctgta
     4861 gagcgcgcag tggttcttca tcaccagaaa ggccataaat atgaatgcca gcaggaaaac
     4921 aaccaggggg aaaaagtatc tctgcgagga gaaaagaccg gtggaagccc acaagaagct
     4981 ttccgtatct atccaataga acagggtcat tggatagatg aatatctgca ctccgttaaa
     5041 atcaccgaag agcaggctga atatacggat caggatcttg tgcattgttt ttttagatgg
     5101 tcctttataa cgctgggcac gagccaaatc ctgcggatgc catatttgtc aatgccaatc
     5161 ttggggtcct gtgcgccatc atcttcacct tcagagggtc ttggtgcctt tggcggaaaa
     5221 agcgttgacg tcgagggagg aaaacttccc ctctttcagc ttgggaaggg cagccgcgcc
     5281 aaccatggcg gcgttgtcca cgcagaattg ttgagcgggg atgtaaacac acgcaccctg
     5341 ggcctcagcg gcttctctga tctgtttccg cagggccgag ttggcggcaa cccctccggc
     5401 gaggaggatt gtttccaagc cttgctgcct ggcgtatttg agagttttgc cgatgagcgg
     5461 gtcaattatg gcttgctgga tggaggctgc caggtcatgc aggtgcgcgt ctatgaagtc
     5521 ccggtctttt cgggccagat actcaagcac tgctgttttc aatccgctgt agctgaaatt
     5581 caggtcgtcc ctgtttttca gggcacgggg aaaatggtgg aaggccgggt ctccgccctg
     5641 ggcggccttg tcgagttccg gtccgccggg gaagcccagt cccagcagct tggcggtctt
     5701 gtcgaaagtc tctccggcag cgtcatccag ggttttgccc accaccttga aatcagtcag
     5761 ggtgctgaag tgcaccaatt cggtatgtcc gccggaaacc accagggcca ggaagggagg
     5821 ctggagccac ggatgctcga tgaaattggc aaagatgtgc gaaagcatgt gattgaccgt
     5881 gatcagcggt ttctgccagc tccaggcaag gcttttggcg aaagacaagc ccacgagcag
     5941 cgaaccgatc aaaccggggt tgatagaaac cgccagagct gaaatgtcct ctttcttcag
     6001 cccgcttttg cgcagggccg cttccgtgag gtggagtatg ttctgcaaat gcaggcggga
     6061 agcgagttcc ggcaacacgc cgccgaaatc ggcatggtcg gcttgggtgg agacaaggtt
     6121 gcagataact tcatagccat cgtccacgat cgccactgag gtgtcgtcgc aggaagattc
     6181 gaaggccaga attgggttca tcgcgaggca cgcacctgtt tgggggtgat gtccagcacg
     6241 gtgatgcctt ccggaacctc gtatctcagc ttgtattggc ccccagcgtc gggttggctg
     6301 gcagcaaaaa cacgcacacc gcttggaagg ccggccagaa gggaggcatt gcctttcact
     6361 ttcagggtgg cctggggtgg gaatgtttta actccctgcg gcggagttat cacgaggtct
     6421 gtaaggattt tggtttgaag ctgtccttct tcagaatcgg attgggatgg ttccgttccc
     6481 gcggcagagg gggctttttt cccgtttttg gctgctgaat gcgcgttttc agcccgcgcc
     6541 tggcctgctt cgctgagatg ctccgccagg gtgggaggca tcactcccag caccttcaca
     6601 ttcagctttt cggggacatt cagggccaga tagtccgccg cgtttccagc ccaaaaatcc
     6661 gccgcattca tctggatgtg ggcctgcgaa tatctgaggc gcaggatgtc gagtccccgt
     6721 ccatagacag aggtggctat cctgcggggc ggctggcgca gcgaatcttc cccggtagcg
     6781 ttcaccagtt tcagcggaag gctggttttg ctctcgtgct gcgaaagcaa cgtgatttgc
     6841 agccagaaga atacagccat caccagcgcc acgattttta ggccaaggtt gtttttcatc
     6901 atc
//
LOCUS       DAOOWR010000050         6708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11459_length_6708_cov_3.143244, whole genome shotgun sequence.
ACCESSION   DAOOWR010000050 DAOOWR010000000
VERSION     DAOOWR010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11459_length_6708_cov_3.143244"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2190)
                     /gene="scpA"
                     /locus_tag="PLB85_02195"
     CDS             complement(61..2190)
                     /gene="scpA"
                     /locus_tag="PLB85_02195"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOU72166.1"
                     /translation="MKPDFTTLNPSFAAEPKAREAVASKAWRTNEQLDVKPLYTKADL
                     DNLQHLDYLSGLPPFLRGPYPSMFVQRPWTIRQYAGFSTAEESNAFYRRNLAMGQKGL
                     SIAFDLATHRGYDSDHPRVIGDVGKAGVAVDSILDMKILFDRIPLDKMSVSMTMNGAV
                     IPIMAFYIVAAEEQGVKPEQLNGTIQNDILKEYMVRNTYIYPPEASMRIIADIFKYTS
                     RNMPKFNSISISGYHMHEAGATADLEMAYTLADGLEYVRTAVNAGLDIDLIAPRLSFF
                     WAEGMNYFMEVAKLRAARVLWAKIIKGFGAKNPKSMALRTHSQTSGWSLTEQDPYNNV
                     IRTCVEALAATLGHTQSLHTNSLDEAIALPTDFSARIARNTQLFLQHETGITKVIDPW
                     AGSYYLESLTDALMKRSWGHIMEVEKLGGMAKAIETGLPKLRIEEAAARRQAHIDSGE
                     DTIVGVNKYRLEKEDPMEILEVDNSAVRESQIARLAKLRAERDEARVKACLGAITQAA
                     KDKTGNLLELAVDAARARASLGEISSAMESVFGRHQASIKLINNVYSSEYAKMDEIEQ
                     VRALTDRFADREGRRPRILVAKMGQDGHDRGAKVVATAFADMGFDVDMGPLFQTPDET
                     ARQAVENDVHIVGMSSLAAGHKTLLPQLVEELAKLGREDILIVVGGVIPPQDYDFLLD
                     KGATAIFGPGTKLPECATLLLEKLLQD"
     gene            complement(2187..4076)
                     /locus_tag="PLB85_02200"
     CDS             complement(2187..4076)
                     /locus_tag="PLB85_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU72167.1"
                     /translation="MKVELQELDLRKEFPPPSLEEWKQAVVASLKGADFDKVMRTKTY
                     EGITLKPIYTKEDVAGLGFTDALPGEAPHQRGNAPQGFASQGWKVAQAQTTQDLAALN
                     REILDELSRGLSAVNLVLKHDDFPAGIELRSAEDLATALKGVDLKAASLMVQMDMDDP
                     DLFSWLEEFCAQTKQDLSQLDGCLGFDPTSEFARKGFLNLPLEDVWSRVGAEVARRVE
                     KAPRLRCLSIDATVYASSGASAVQELAFALSTAIGYIQGLQASGLEIDKIAPLFQVKF
                     SLGSNLFMEIAKIRAFRMLWSEMVKAFGGNDESRRIWIHGKTSTFNKSGYDLYVNMLR
                     TSTEGFAGVVAGVDSLEIDRFDATLDVEATEFSKRLARNQQLILAEEAHLSRVADPAG
                     GCYYIESLTADLASQAWKMMQELEAGGGMIRGLKAGTIHTMIDAVAQARIDAVHKRRD
                     VFVGVNMYANPDDALPALAEASVDDSKAAVRLERGALPKRRAVEALEQFRSRMAAYGK
                     KVFLITMGSLAEYKARADFATGFLQVGGFIVVEGTGYADVQSAVEAARDYEAVCICST
                     DDKYPELVPALCAKLTDKIKILAGYPADQVEAHKQSGIDVFIHLRANLLDTLQYLAAR
                     MGVQK"
     gene            complement(4084..5358)
                     /locus_tag="PLB85_02205"
     CDS             complement(4084..5358)
                     /locus_tag="PLB85_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU72168.1"
                     /translation="MTSIPCFLEADLHGKTVLVRMDHNVVKKGKIKDPMRIEATIPTL
                     LHIFKKGGRPILMSHVGRPYNKATGAITISEGEAVGPIVKYLEDKLQLRGLIPDCPAE
                     GEKGITDLSPIGAAVSAMKEGKADFVYLPNTRWFKGEESKNEDSDALAAKLASFADLF
                     INDAFGSWQPHASTFNITKYLPSYAGLLMLKEIANLQKVFEPRKPLVSVVAGSKFDTK
                     IGPLSSLIKISDKLVLGGVLYNAYLAVKYQIGIKGLEPSDLEMAEKFLQEAGPLKDRI
                     VEPPYIIESDSFENAESWRVHKISDLKPGTQLNYVLDVAPESFSEPAVQEVFAGAATV
                     FVNAVMGYTALFTEGTKAMYSLIDKNTGAQKLFGGGDTIQDFRGLLPGIFAKAVNDPQ
                     YYFFTGGGAVLDSIVNGSPYGMKPVQALIKES"
     gene            complement(5467..5967)
                     /locus_tag="PLB85_02210"
     CDS             complement(5467..5967)
                     /locus_tag="PLB85_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOU72169.1"
                     /translation="MRYRITKKSLLSMALGLIVLLAVRFLSAKEIRGHVVSIQDGDTI
                     TVLQGRTTCKVRLAAVDCPEKGQAFGNVARQFTSDLVFGKAVKVKYEELDRYNRYLGT
                     VYIGGKNLNKELLKAGLAWHYKQYSKDPVLAALEVKARQDRKGLWSDPKAIPPWEFRR
                     LKREKA"
     gene            complement(6325..>6708)
                     /locus_tag="PLB85_02215"
     CDS             complement(6325..>6708)
                     /locus_tag="PLB85_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72170.1"
                     /translation="EIAAAFAQPRIPQFRFLRSETLQGKGRQPTGEIIGIAIPDNLRT
                     GVESRIAEFFQAERHIFTKSTPTRSKEYDLRRIVTSSEWNGSRLLIGKSLASPSLFDV
                     LAELLALDKTELYALSVTRYDWLFK"
BASE COUNT         1249 a         1867 c         2007 g         1585 t
ORIGIN      
        1 aggcgaattc cttcccgccc tcagcgggtg cccattccgg cttgcgatgc ctggtcacgc
       61 tcaatcctgc aacagttttt ccagcaacag ggtggcgcat tcaggcagtt tggtgccggg
      121 cccgaaaatg gctgtggcgc ctttgtccag caggaaatcg tagtcctggg gcggaatcac
      181 gccccccacc acgatgagga tgtcctcgcg gccaagcttt gccagttcct ccaccagttg
      241 cggcagcagg gtcttgtggc cggcggccag tgaactcatg ccgacgatgt ggacgtcgtt
      301 ttccactgcc tggcgggctg tttcgtccgg ggtctggaac aggggtccca tgtccacgtc
      361 gaaacccatg tcggcaaaag cggtggcaac cacctttgcg ccgcggtcgt ggccgtcctg
      421 gcccattttg gcaaccaaga tgcgggggcg gcgtccctcg cggtcggcaa acctgtctgt
      481 gagggcgcga acttgttcta tttcatccat tttggcatac tcgctgctgt aaacgttgtt
      541 gatgagtttg atggaagcct ggtggcgtcc gaagacagat tccatggcgc tggaaatctc
      601 gcccaaggaa gctctggccc gggcagcgtc caccgccagc tcgagcaggt ttccggtttt
      661 gtctttagct gcctgggtga tggcgccgag gcaggctttc accctggctt cgtcgcgttc
      721 agcacggagt ttggccaggc gggcgatctg ggattcgcgc acagcggagt tgtccacttc
      781 caggatttcc atcgggtctt ccttctccag gcggtatttg ttcacgccca cgatggtgtc
      841 ttctccggaa tcaatgtgcg cctgccttct ggcggctgct tcctcgatgc gcagtttggg
      901 caggcctgtt tcgatggcct tggccatgcc gcccagtttc tccacttcca tgatgtggcc
      961 ccaagagcgc ttcatcaggg cgtccgtaag gctttccaga tagtaggagc cggcccaggg
     1021 gtcgataacc ttggtgatgc cggtctcgtg ctgcaggaag agctgggtgt tgcgggcgat
     1081 gcgggcgctg aaatcggtgg gcagggcaat ggcttcgtcc agggaattgg tgtgcaggga
     1141 ttgcgtgtgt cccagcgtgg cggccagggc ttccacacag gtgcggatca cgttgttgta
     1201 ggggtcctgc tccgtgaggc tccagccgga ggtttgggaa tgggtgcgca gcgccatgct
     1261 cttcgggttt ttggccccaa aacctttgat gatcttggcc cagagcactc ttgcggcacg
     1321 gagcttggcc acttccatga agtagttcat gccctcggcc cagaagaatg acagccgggg
     1381 cgcgatcaaa tcgatgtcca ggccagcgtt tacggccgtg cgcacgtatt ccaggccatc
     1441 ggccaaagta tatgccatct ccaggtcggc ggtggctcct gcttcatgca tgtgatagcc
     1501 ggaaatgctg atgctgttga acttaggcat gttccgcgac gtgtatttga agatgtcagc
     1561 gatgatgcgc atggaggctt ccggtggata gatgtaggtg ttgcgcacca tgtattcctt
     1621 caggatgtcg ttctggatgg tgccgttaag ctgttccggc ttcacaccct gttcctcagc
     1681 ggccacgatg tagaaggcca tgatggggat cacggcaccg ttcatcgtca tggaaacact
     1741 catcttgtcc agcggtatcc tgtcgaagag gattttcatg tccagaatgc tgtccacagc
     1801 caccccagct ttgcccacgt caccgatcac gcggggatgg tcggaatcgt atccgcgatg
     1861 ggtggcgagg tcaaaggcga tggagaggcc tttctggccc atggccaagt tgcggcggta
     1921 aaaggcgttg gattcctcgg ctgtggaaaa acccgcgtat tggcggatgg tccagggccg
     1981 ctggacgaac atggaaggat aaggcccccg caggaaaggc ggcagaccgg aaaggtaatc
     2041 taaatgctgc agattgtcca ggtcagcctt ggtgtaaagc ggcttcacgt ccaactgttc
     2101 gttggtgcgc caggctttgg atgcgactgc ttcccgcgct ttgggttccg cggcgaagct
     2161 gggattcaga gtggtgaaat caggtttcat ttttgcaccc ccatgcgggc ggccaggtat
     2221 tgcagggtgt ccagcaggtt tgcccgcaga tggatgaaga catcaatgcc gctttgtttg
     2281 tgagcttcca cctggtcggc cggatagcct gccaggattt ttatcttgtc agtcagttta
     2341 gcgcagagcg cgggcaccag ttcgggatac ttgtcgtccg tgctgcagat gcagacagct
     2401 tcgtagtccc gggcggcctc cacggcggac tgaacatcgg cgtagccagt tccttccacc
     2461 acgatgaatc cgcccacctg caggaagcct gtggcgaagt cggcgcgggc cttgtattct
     2521 gccaggctgc ccatggtgat caggaacacc tttttgccat aagccgccat ccgggagcgg
     2581 aattgttcca gagcttccac cgctcggcgt ttcggcaggg cgccccgctc gaggcgcacg
     2641 gcggctttgc tgtcatcaac agaggcttcc gccaaggccg gcagagcgtc atccggattg
     2701 gcatacatgt tcacgcccac gaaaacatct cgccttttgt gcacggcgtc gattcttgcc
     2761 tgggccacag cgtcgatcat ggtgtggatg gtcccggctt tgaggccgcg gatcattccg
     2821 cccccggctt ccagctcctg catcattttc caggcttggc ttgccagatc cgcggtgagg
     2881 ctttcgatat aatagcatcc gcctgcgggg tcggcaaccc tgctcagatg ggcttcctcg
     2941 gccaggataa gctgttggtt gcgggcaagc cgtttggaaa actcggtggc ctcgacgtcc
     3001 agcgtggcgt cgaaacggtc tatctcaagg ctgtccaccc ctgcaacgac cccggcaaac
     3061 ccttccgtgc tggtgcgcag catgttcaca tagaggtcat agccgctttt gttgaaggtg
     3121 gaggtttttc cgtggatcca gatgcggcgg gactcatcat tgccgccgaa agcttttacc
     3181 atctcgctcc acagcatccg gaaagccctg atcttggcta tttccatgaa caaattggaa
     3241 ccgagggaaa acttcacctg aaacagcggg gcgatcttgt cgatttccaa gccgctggcc
     3301 tgcaagccct ggatataacc gatggcggtg gacagggcga aagcgagttc ctgcacggct
     3361 gaggctccgc tggaggcata gaccgtggcg tcgatggaaa ggcagcgcag cctgggagct
     3421 ttttccaccc tgcgcgcaac ttcagctccc acccggctcc aaacatcttc cagaggcagg
     3481 ttcagaaatc ccttgcgggc aaactcgctc gtgggatcga agccgaggca gccgtcaagc
     3541 tggctcaggt cctgtttggt ttgggcgcaa aactcctcca gccaggagaa gaggtctgga
     3601 tcgtccatat ccatttgaac catgagcgac gcagctttca ggtccacccc tttcagggca
     3661 gtggccaggt cttccgcgga gcggagctcg attccagcgg gaaaatcgtc gtgtttcaga
     3721 acgaggttaa cggcagaaag tccccgggaa agctcatcca gaatttcccg gttcagggct
     3781 gccaggtctt gggtggtctg tgcctgggcc actttccagc cttggctggc aaagccctga
     3841 ggcgcgtttc cccgctggtg gggagcttcg cccggcaggg cgtccgtgaa gcccagtccg
     3901 gccacgtctt cctttgtgta tatgggtttc agggtgatgc cttcgtaagt tttggtgcgc
     3961 atcaccttgt cgaaatcagc gcctttaagg ctagccacca cggcctgttt ccattcttcc
     4021 aggctgggcg gcggaaactc cttgcggagg tccagttctt gaagttccac tttcactctc
     4081 ccttcaggac tctttgatga gggcctggac aggcttcatg ccgtagggcg agccgttcac
     4141 gatggagtcg aggacagccc cgccgccggt gaaaaagtag tattgaggat cattcaccgc
     4201 ttttgcgaaa atgccgggca gcagacctcg gaaatcctgg atggtgtcgc cgccgccgaa
     4261 gagtttctgc gcaccggtgt tcttgtcgat caggctgtac atagctttgg tgccctcggt
     4321 gaagagggcg gtgtagccca tcacggcatt cacgaaaacg gtggcggccc cggcaaagac
     4381 ctcctgcacg gcgggttctg aaaaggattc cggggcaacg tccagcacat agtttagctg
     4441 ggtgccgggc ttcaggtcgg agattttgtg gactctccag ctttcagcgt tttcaaagct
     4501 gtccgattct attatataag gaggttcaac gatgcggtct ttcagggggc cggcttcctg
     4561 caggaatttt tccgccattt ccaggtcgga gggttccagg cccttgattc cgatttggta
     4621 tttcacagcc aggtaggcgt tgtagagaac cccgcccagc acgagtttgt cgctgatttt
     4681 gatgagggag gaaaggggtc ctatcttggt gtcgaatttc gagccggcca ccacggaaac
     4741 caggggcttg cggggctcga agaccttctg caggttggcg atctctttca gcatcagcag
     4801 tccggcatag gaaggcagat acttggtgat gttgaaggtg gaggcatgcg gctgccagga
     4861 accaaaagca tcattgatga agaggtccgc gaaagaggcc agcttggcgg ccagggcgtc
     4921 cgaatcctcg tttttgctct cttcgccttt gaaccagcgg gtgttgggca gatacacgaa
     4981 atccgccttg ccctctttca tggcggaaac cgctgcgccg atggggctaa ggtcagttat
     5041 ccccttttcg ccttcagccg gacagtcagg aatcagtccg cgcaactgga gtttatcctc
     5101 cagatactta acgatggggc caaccgcctc gccttcggaa atggtgatgg ctccggtggc
     5161 tttgttgtaa ggcctgccca cgtggctcat caagatgggg cggccaccct tcttgaaaat
     5221 atgcagcagg gtggggatcg tggcttcgat cctcatcggg tctttgatct tgcctttttt
     5281 cacaacattg tggtccatgc gcaccagcac ggtttttccg tggaggtccg cttccaggaa
     5341 acagggaatg cttgtcatgg ctcggttctc cttgtatgtc ttatatatca aatacttagc
     5401 ggatatcgga taacggcggc agagaaaatg ccatttggaa gcagtttgcc gaaaggggcg
     5461 gcagcgtcaa gctttttctc ttttcaggcg gcggaactcc caaggcggga tggctttggg
     5521 atcagaccag aggcctttcc tgtcctgcct ggccttcact tccagggcag ccaaaaccgg
     5581 gtctttgctg tattgcttgt aatgccaggc caggccggct ttcagcagct ccttgttcag
     5641 gttcttcccg ccgatgtaaa cggtgcccag atatctattg tagcggtcga gttcctcata
     5701 tttcactttc acggccttgc caaaaacgag gtcggatgtg aattgtctgg caacattgcc
     5761 gaaagcctgc cccttttccg ggcaatccac cgctgccagg cgcactttgc aggtggttct
     5821 gccctgcaaa accgtgatgg tgtcgccatc ctggatggag accacgtgcc cccggatttc
     5881 tttcgcggaa aggaaacgaa cggccagcag gacgatcagc cccagcgcca tgctcagcag
     5941 ggattttttg gtgatcctgt atctcatgga gcctttgtcg ctttggtagc gcttctgtca
     6001 atggaaattc cgccaacggc attcccaaat aaccgaatct gcccgctttc catttgcctc
     6061 gtttcgccct aatctgctct gtatgatggc ttttcaggtt ccacctcgtt acctccgtgt
     6121 gatgtttctc gttcatcccc ggcgaatgcc tgatttgtcg tcggtgcttt ccccatttac
     6181 cccttcccac tcacttccca ttcacttccc gcttagttcc cgcctttcaa acgggaacta
     6241 agcgggaggc gaaaaagagg tggatgggaa agggcaaagg aagcgggcac aggaaaccaa
     6301 gttacgcccc tttcagccta aaagctattt gaacagccag tcatagcgcg tgaccgaaag
     6361 cgcgtaaagc tctgttttat ccagcgccag cagttccgcc agcacatcga acaaggaagg
     6421 gctggcaagg cttttgccga tcagcaggcg cgagccgttc cactcgctgg aggtgacgat
     6481 gcgcctgagg tcatattcct tgctgcgggt gggggtgctt ttggtgaaga tatgccgttc
     6541 ggcctggaag aattccgcaa tgcggctttc cacgcccgtc cgcaggttat cgggaatggc
     6601 gatgccgatg atttcgccag tgggctgcct gcccttgcct tggagggttt cgctgcgcag
     6661 gaagcggaat tggggtatgc gcggctgggc gaaggccgcg gctatttc
//
LOCUS       DAOOWR010000051         6629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11666_length_6629_cov_4.082446, whole genome shotgun sequence.
ACCESSION   DAOOWR010000051 DAOOWR010000000
VERSION     DAOOWR010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11666_length_6629_cov_4.082446"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="ribH"
                     /locus_tag="PLB85_02220"
     CDS             complement(<1..398)
                     /gene="ribH"
                     /locus_tag="PLB85_02220"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOU72171.1"
                     /translation="MKVIEGNLVSKGYRFALVVSRFNELVSRKLMEGAMDCLLRHEVL
                     EDDITVIWVPGAFEIPQMAKAAAQSDSYDAVICLGAVIRGGTPHFEYVAAEVSKGVAQ
                     VALSQSKPVIFGVLTTDTIEQALERAGTKAG"
     gene            790..1263
                     /locus_tag="PLB85_02225"
     CDS             790..1263
                     /locus_tag="PLB85_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU72172.1"
                     /translation="MNRLFQPELVKIVDGFESKSDCLAYMTDLLAESGCLDLPERFLA
                     AVKGREDIMSTGIGKGVAIPHARDLTVTCIKIAVCLVREPLEFDSVDDLPVQLVFMIA
                     VPQKAGKEYMKILRSLSEYLRNGENRNTLINATCERELYHDVLTIEEVITDSINA"
     gene            1256..2176
                     /locus_tag="PLB85_02230"
     CDS             1256..2176
                     /locus_tag="PLB85_02230"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU72173.1"
                     /translation="MLSFVLLPLLAVNDEAGTTGFASLKNVYSARAIAMGQALTGEAG
                     NPDGLHFNPASIIKIDGNEVGTTYTNSFVGSQGGQLQYLWPKDRFTAWGFALKYMNMG
                     RMERTEVDQNGDLVSTGETFGANNIVASASLAKYISDAVDLGGTLKVIYDQIDSSSST
                     AAMLDLGLIHHPASEKLKVGISLRNIGLQITRYTGSDYKEKLPFTFAAGMTYDFNPRL
                     MASLDIVKATGEDIVAKAGVEYELTPAFDLRGGFRTNAGDGYNGGTFAFLSGLSLGVG
                     WNWNNYRVDYGVSSYGDLGWINQISLSYEF"
     gene            2197..4281
                     /gene="glyS"
                     /locus_tag="PLB85_02235"
     CDS             2197..4281
                     /gene="glyS"
                     /locus_tag="PLB85_02235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOU72174.1"
                     /translation="MTRDFLMEIGTEEVPAPHLQPAVEFIQSSFQNLMRDVGLQYSEL
                     MTGSTPRRMFLIARQIQSTQPDIQVNKTGPAKKIAYDADGNLLPAALGFLKKNNAEAK
                     DLQIETTDKGEFIALKYIKPGRNTAEILREWIPDLLNHIPYPKTMIWNSSRLALSRPL
                     RWLCVLWGDEVLEVEAGGVKSGKHSFGNRFLGLDRPLEINSADEYLQVLSSNAVMADR
                     ATRREKLVAELASVNPGMGFKVVEDERLTDTVTDLVELPTAVVGEFSLEFLSLPEKII
                     TSTISQNQKYYSVQDAEGRLSNKFVFISNGDPEYSDLIRKGNEKVVAARLADALWYYQ
                     EDTRKPLEAYVEELHEVVFQSKLGTLADKTERVGQITGEICRRLCLDEENTALAKRAA
                     LLCKADLVTTMLGEKEFTRLQGYIGKHYALASGEDSRVAEAIHEHYQPRGTNDGLPQT
                     LTGAIVAVADKMDSVCGIIGIGQVPTGSADPFALRRAANGVVQIIVERGWSINLSELI
                     DYTLNLVEQRSELTSTAHSDVQAFFRLRVEWLLRQLGLDYDVIDSLMHLSLGNLPDLK
                     TRGEVLQSSRSREDFLRLVIGFKRVANIIEKVERVPALEPSLFEEEAEKELYSSLQIL
                     RGKNDASLATRNYQQAITLLVGYGANIDSFFDAVLVNCDDPTLRGNRYALLSEVKNEF
                     LRVADISRIVID"
     gene            4311..5318
                     /gene="pta"
                     /locus_tag="PLB85_02240"
     CDS             4311..5318
                     /gene="pta"
                     /locus_tag="PLB85_02240"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOU72175.1"
                     /translation="MDILTELKAKAKLIEGQIVLPESADTRTLQAAHAILAEGLADIV
                     LVGKPEEIKTREKTLELDLSRATVIDPANFPEIGKFADFYYEKRKEKGVTREQASQTV
                     LNELYFGTLLVKFGYADGMVAGAANTTADVLRAALQVVGVTSGIKTVSSCFIMVVNDY
                     MGEDRVYLFADCAVVPDPDPEQLADIAFSTASTRRAILGDEPKVALLSFSTRGSAQHE
                     NVGKVQQTVKILGERQVDFDFDGEMQLDAAIVPNIAKMKAPDSKVAGQANTLIFPDLQ
                     SGNIGYKLVQRFAKAEAIGPIIQGLDAPVCDLSRGCSSEDIVHTCVLVLLMAQNNKRK
                     G"
     gene            5323..6525
                     /locus_tag="PLB85_02245"
     CDS             5323..6525
                     /locus_tag="PLB85_02245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU72176.1"
                     /translation="MAIKISNRIKLVKPSPTLTLSARAKEMMASGIDVVNFGVGEPDF
                     NTPEYIKKSAHAALEANFTRYTANPGIIELRQAICDKLERDNGLAYEPKEVLVSPGAK
                     ASIVNILIAVCDAQDQVLMPAPYWVSYPYQAMLANAEPVIVPTFEEDSYKLTADSLNQ
                     AIAANPCAKVLLLNSPNNPTGAVYTRQELEAIAAICVKNDILVVSDEIYERLVYDGIK
                     HISIASLNEEIKARTVVINGVSKAYAMTGWRLGYAAGPAHIIAAAGRVQAHATSCVNS
                     ITQKACVTALAEEDDSIENMRAEFEKRRDFLYAELSKLPHVSCFKPQGAFYIMPNISW
                     YLSNNSQSIKDGDQFCQALLEKHHVALVSGDSFGIPSNVRFSYANSMENLQKGVERFA
                     KFLGELAR"
BASE COUNT         1638 a         1871 c         1765 g         1355 t
ORIGIN      
        1 ccggctttgg tgccggcgcg ttccagggct tgctcgatag tgtctgtggt aagcactccg
       61 aaaatcacgg gcttggattg gctcagggca acctgggcca cccctttgct aacctccgcc
      121 gccacatatt caaaatgggg tgtgccgccc cggatcaccg cgcccaggca gattacggca
      181 tcgtaactgt cgctctgcgc cgcggctttg gccatctgcg ggatttcaaa agcaccgggc
      241 acccaaataa ccgtgatatc gtcttccaag acttcgtgac ggagcaggca gtccatcgcc
      301 ccctccatca gcttgcggct caccagttcg ttgaaacggc ttaccaccag ggcgaatctg
      361 tagcccttcg aaaccaggtt accttctatt accttcattg tttttgctcc ttttcccaag
      421 gatttttcca caaaaaacca gaatattcaa agcacagatt caatcaagga aaatctttcg
      481 ctaaggagta agagctgtat cctgccagtc aggccctgag cgctcgcgac cgtccctctc
      541 ccccgcctcc cgccaaatac tcgcatcgaa tgcgggcatc atgcgggagg caataccggg
      601 gcctgaggta gggcgttaac ggccagaagg catcgcttca acctggttgc ttcacacacc
      661 cgctgtcctt gcaaccctcc gggatcgaaa tcaccggggt aaacacctaa tttgaaaggg
      721 agcctaaaat ttggttgacg gaaacggagt atcaaaaaat catgtttgaa tcaaaaatat
      781 ggtgcgaaga tgaacagact tttccaacct gagttagtaa agatcgtcga tggattcgaa
      841 tcgaagtccg attgtctggc ctatatgaca gacctactgg cggaaagcgg ctgcctggat
      901 ttaccagagc ggtttctggc cgcggtgaaa ggccgtgagg acatcatgag caccggcatc
      961 ggcaaaggcg tggccattcc tcacgcgcga gatctgaccg ttacctgcat caagatcgcg
     1021 gtctgccttg tccgcgagcc gctggaattt gatagcgtgg acgacctgcc ggtgcaactg
     1081 gtgtttatga tagcggttcc ccaaaaagcc ggcaaagaat atatgaaaat cctgcgctcg
     1141 ctctccgaat acctgaggaa tggcgagaac cgcaacaccc taatcaacgc aacctgcgaa
     1201 agggaattat accatgacgt gcttacgatt gaagaagtca ttactgacag cattaatgct
     1261 tagctttgtt ttgctgcctt tgctggcggt gaacgatgaa gccgggacca ccgggttcgc
     1321 cagcctgaaa aacgtctatt cggcccgggc catcgccatg ggccaggctc tcaccggcga
     1381 agccggcaat ccggacggtc tgcatttcaa cccggcctcg atcatcaaaa tcgacggaaa
     1441 cgaggtcggg accacctata ccaactcttt cgtgggttcg cagggcggcc aattgcagta
     1501 tctctggccc aaagaccgct tcacggcctg gggttttgcc ctcaaataca tgaacatggg
     1561 caggatggaa cgcactgagg tggaccagaa cggcgacttg gtttcgaccg gagagacctt
     1621 tggggccaac aacattgttg ccagcgcctc cctggccaaa tacatctccg acgctgtgga
     1681 ccttggcggg accctgaaag tgatctacga ccagattgac agcagttcct ccacagccgc
     1741 gatgctggac ctgggcctca tccaccatcc cgcaagtgaa aagctcaaag tggggatcag
     1801 cctgcgcaac atcggcctgc agataacccg ctacaccggt tccgattata aggaaaagct
     1861 gcctttcacc ttcgccgccg gcatgacata cgatttcaat cccaggctga tggccagcct
     1921 cgacattgtc aaagctaccg gagaagacat agttgccaaa gccggggttg aatatgaact
     1981 gacgcccgct ttcgacctgc ggggaggttt caggaccaat gccggtgacg gctacaacgg
     2041 cggcactttc gcctttcttt caggattgtc cctgggcgtg ggctggaact ggaacaatta
     2101 ccgggtcgat tacggcgttt cttcatacgg agacctgggc tggatcaacc agatcagcct
     2161 cagctacgaa ttctgaaccc tgttccggtg aaagctttga cgcgtgattt tctgatggaa
     2221 attggaaccg aggaggttcc cgcaccccat ttacaaccgg cggtggaatt catccagtcc
     2281 tcgtttcaga atctgatgcg ggacgtgggt ttgcaatact ctgaactgat gaccggttcg
     2341 accccccggc gcatgtttct catcgcgcgc cagattcaga gcacccagcc ggatatccag
     2401 gtgaacaaaa ccggcccggc aaaaaagata gcctacgacg cggacggcaa ccttctcccc
     2461 gcggctttgg gcttcctaaa aaagaacaac gccgaagcca aagacctcca aatagaaaca
     2521 accgacaaag gcgaattcat tgcccttaaa tacattaaac caggccggaa cacggcggaa
     2581 atcctgcggg aatggattcc ggaccttctg aaccacattc cctaccccaa aacaatgatt
     2641 tggaacagtt cccgccttgc cctgtcccgt ccgttgcgct ggctctgtgt gctttggggg
     2701 gatgaagtcc ttgaggtgga agctggtggg gtcaagagcg gcaaacatag cttcgggaac
     2761 cggtttctag ggcttgacag gccgctggag ataaactccg cggatgagta tcttcaggtt
     2821 ctttccagca atgccgtgat ggcagacaga gccacccgac gggaaaaact ggtggctgag
     2881 cttgcctccg tgaacccggg aatgggattc aaggtggttg aagatgaacg ccttacggac
     2941 acggtaaccg atctggtgga attacccact gcggtggtgg gtgaattcag cctggaattc
     3001 ctttcactgc ctgaaaagat catcacctca acgataagcc aaaaccagaa atactattcc
     3061 gttcaggacg cggaaggccg tctgagcaac aaattcgtgt tcatctcaaa cggcgacccc
     3121 gagtattcag acctcatccg caaaggcaat gaaaaggttg tggcagcgag gctcgcggac
     3181 gccctctggt attatcagga agacaccagg aaacccttgg aagcttatgt ggaggagtta
     3241 cacgaagttg tgttccaatc caagctcggc acgctggcag acaagacgga gcgcgttggc
     3301 cagatcacag gggaaatctg ccgtcggctg tgcctcgacg aagaaaacac ggccctggcc
     3361 aaacgggcag cactgctttg caaggcggac ctggtcacca cgatgttggg agagaaagaa
     3421 ttcaccaggc tgcagggata tatcggcaag cattacgccc tggccagcgg tgaagacagc
     3481 cgggtggcgg aagccattca cgagcattac cagccccggg gcaccaatga cgggctgccc
     3541 caaaccctca cgggggcaat tgttgccgtg gccgataaaa tggacagcgt gtgcggaata
     3601 atcggcattg gccaggtccc aaccggctcc gcggacccct ttgccctgcg cagagcggcc
     3661 aacggagtgg tgcagatcat agtggaacgc ggctggagca tcaatttatc cgaacttatc
     3721 gattatacct tgaacctggt ggaacaacgg tcagagctta cttccaccgc ccacagcgat
     3781 gttcaggcct ttttccgcct gcgggttgag tggttgctgc gccagttggg tttggattat
     3841 gacgtaatcg acagcctgat gcacctttca cttggtaacc tgcctgacct aaaaacacga
     3901 ggggaggttt tgcaaagctc ccgcagccgg gaagactttc tccgtttggt gatcggcttc
     3961 aaaagggtgg ccaacatcat cgaaaaagtg gagcgcgttc cggctctgga accatcgctg
     4021 tttgaagagg aagcggaaaa agagctgtat tcctctctgc aaatcctgcg gggaaaaaat
     4081 gacgccagcc tggcaacccg caattatcag caggcgataa cccttctggt tggctacggc
     4141 gccaacatcg acagcttttt cgacgccgtg ctggtcaact gtgatgatcc aaccctgcgt
     4201 ggaaaccgct atgccctctt gagcgaggtc aaaaatgaat tcctgcgcgt ggcggacatt
     4261 tcgcgcatcg tgatagattg atagataatg ataaaaatgg agcatagagt atggatatcc
     4321 tcaccgagtt aaaggccaag gccaaactca tcgaagggca gatcgtgctg cctgaaagtg
     4381 ccgacaccag gacgcttcag gccgcgcatg caattttggc tgagggtttg gcagacattg
     4441 tcctggtggg caaaccggaa gagatcaaaa cccgggaaaa gactctggaa cttgacctca
     4501 gccgggccac tgtgatcgat cctgccaact ttccagaaat cggcaaattc gcggatttct
     4561 attatgagaa gcgcaaagag aaaggcgtca ctcgcgagca ggcaagccag acagttctga
     4621 atgaactgta ttttggcacc ctgttggtga agttcggata cgctgatggc atggtggccg
     4681 gcgccgccaa caccacagcc gatgtcctgc gcgcggctct gcaggtggtg ggcgtcacca
     4741 gcggcatcaa aaccgtttcc agctgtttca taatggtggt gaatgactac atgggggaag
     4801 accgggtcta tctcttcgcg gattgcgccg tggtgcctga tcctgacccg gaacaactgg
     4861 ctgatatcgc attcagcact gcctcaacgc gcagagccat tttgggcgac gaacccaagg
     4921 tggctttgct gtcattttcc acccggggca gtgcccagca cgaaaatgtg ggaaaagtcc
     4981 agcaaacggt taaaatcctg ggcgaacgcc aggttgattt cgatttcgac ggcgagatgc
     5041 agttggacgc ggcgatcgtt cccaacattg ccaaaatgaa agctccagac agcaaagtgg
     5101 ccggacaggc caacaccctt attttcccag acctgcaatc cggcaacatc ggctacaagc
     5161 tggtgcaacg ttttgccaaa gcagaagcca tcggccccat catccagggc ctggacgccc
     5221 cggtttgtga cctctcccgc ggctgttcct ctgaagatat agttcacacc tgcgtgctgg
     5281 tgctgttgat ggcccaaaat aacaaaagaa aaggataaag acatggctat aaaaatatca
     5341 aaccgaatca aactggtaaa accctccccc actctcaccc tttcagccag ggccaaggag
     5401 atgatggcct ccggcatcga cgtggtgaat ttcggcgtgg gtgagcccga tttcaatacc
     5461 cccgaataca tcaaaaagtc ggctcatgcc gctcttgagg caaatttcac ccgctacacc
     5521 gccaaccccg gcatcatcga actccgccag gccatttgtg acaagctcga gcgggacaac
     5581 ggactggctt acgaacccaa agaagtgctg gtctcacctg gcgccaaagc ttccatcgtg
     5641 aacatcctga tcgcggtctg cgatgctcag gaccaggtgc tgatgcccgc gccctactgg
     5701 gtcagctatc cctaccaggc aatgctagcc aacgccgaac cggtgatcgt gcccactttt
     5761 gaggaagatt cctacaaact caccgcggat tcactgaacc aggccatagc ggccaacccc
     5821 tgcgccaaag tgctgctgct gaacagcccc aacaacccca ccggagccgt ttacaccagg
     5881 caggaactgg aagccatcgc cgccatctgc gtgaaaaacg acatcctggt ggtttcggac
     5941 gaaatctacg aacgcttggt ttacgacggg atcaagcaca tttccatcgc ctccctgaac
     6001 gaggaaatca aagcccggac cgtggtgatc aacggcgttt caaaagctta tgccatgacc
     6061 ggctggaggc ttggctacgc cgctggcccg gcccatatca tagcggccgc tggcagggtt
     6121 caggctcacg ccacctcctg cgtgaactcc atcacccaga aagcctgcgt gaccgccctc
     6181 gccgaggaag acgactccat tgaaaatatg cgcgcggagt ttgaaaagcg ccgcgatttc
     6241 ctctatgccg aactgagcaa gctgccccac gtgtcctgtt tcaagcccca gggagctttc
     6301 tacatcatgc cgaacatctc ctggtatctg agcaacaaca gccagagcat caaggatgga
     6361 gaccagtttt gccaggccct gctggaaaag caccacgtgg cgctcgtttc cggcgattcc
     6421 ttcggcatcc cctccaacgt aagattttcc tatgccaaca gcatggaaaa cctccaaaaa
     6481 ggcgtggaac gcttcgccaa attcctgggc gaactggcgc gctgaccaga aaagaggacc
     6541 attatgaccg atctggatga aaaataccgc gaacgctggc aggacaaacg caaaaaaacc
     6601 tatgactgga agcgactggc aatcatggt
//
LOCUS       DAOOWR010000052         6610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11709_length_6610_cov_4.218612, whole genome shotgun sequence.
ACCESSION   DAOOWR010000052 DAOOWR010000000
VERSION     DAOOWR010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_6610_cov_4.218612"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1201
                     /locus_tag="PLB85_02250"
     CDS             77..1201
                     /locus_tag="PLB85_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU72177.1"
                     /translation="MHDDVIRYFLELVAIDSESRDERAVIDRLKTDLAALGAEVFEDD
                     AHTLTGGNAGNLHALIPGRADQKPILFCAHADTVKPGLGVKASITAGRIHTDGTTVLG
                     GDDKSGLAEIVMGIKDVVESGEEHAPIEVLVTVSEEVGLLGAKHFDKSKLRAAFGYAL
                     DAHRVGDLVVGAPAQNSIRMVITGKEAHAGVEPEKGINAIRVAAEAVAAMPLGRIDNE
                     TTCNVGIISGGKATNIVPNSVELKGEARSHNPQKLEQVTANIRHAAENAVQRHKYEFG
                     AASLEWESKQEYASFRISDSEAVVQLALDALKDLGIRAEVTVGGGGSDANIINAAGLP
                     MIICGTGMNKVHTVAEDIETEELKRGAAFVAALIRRYSRS"
     gene            1205..1945
                     /gene="dapB"
                     /locus_tag="PLB85_02255"
     CDS             1205..1945
                     /gene="dapB"
                     /locus_tag="PLB85_02255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOU72178.1"
                     /translation="MLDITLIGYGKMGHMIASLAEEHGCRVRSIIDPGQEGCEKEIRP
                     EILADVCIDFSQPDMALANIRQVAAAGKHMVVGTTGWHRHLDEVKELAERHGIGILWG
                     ANFSIGMNLFTRVIQYATSICDKFPMYDVCGWEMHHNQKADSPSGTAIQLAEAILEES
                     GVKDQAVYDKLDRRILKNELHFASLRGGAMPGTHSVIFDCETDSLELTHRVRSRACFA
                     AGALQAAQWISLRKGFYSFNQMMEDLLC"
     gene            1939..2715
                     /gene="dapF"
                     /locus_tag="PLB85_02260"
     CDS             1939..2715
                     /gene="dapF"
                     /locus_tag="PLB85_02260"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOU72179.1"
                     /translation="MLIPFGKVQAQGNDFVMLQLLHTWNTSLPLDALARDICDRRTGV
                     GADGLVLLLADDEADARMLIYNSDGSHALMCGSALRCCASLLNRLTGKTELLIATDSG
                     LKSATVQERQISVNLGKPVLKEKDLSLEGFTGSVVEVGNLHFVSFQNRLEDQELEIGP
                     RLESHSHFQEGVNVEFVRVISCLEIEMKVWERGAGATQACGTGAVASVFTGIGKGLLD
                     NEVWVKMPGGEVCITRLENGEFLLAGTVEHTFSGVYQWRI"
     gene            2703..3332
                     /locus_tag="PLB85_02265"
     CDS             2703..3332
                     /locus_tag="PLB85_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU72180.1"
                     /translation="MENLGSHLQKLREEKGISYDKVFEDLRLREDQLRLIEENRYFEL
                     GHFGFAKAMVHKYAGYLGADLDEVMAEFKVMVPENTKKQPVRGGGSKSSKIMLSPNLL
                     WLLGIILFVIVLGAILWYAHNQGWLKTPEFLKPAAADTTVVSKPRLPQEKEPKVDPMR
                     EVQKEVTKAAAIPSVTPAQPSQGIRQAVQDSTDHIGELMGPSQVNVGLE"
     gene            3445..3930
                     /locus_tag="PLB85_02270"
     CDS             3445..3930
                     /locus_tag="PLB85_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOU72181.1"
                     /translation="MPAFSINEIIEMAVQIERNGYAFYQEATKRKDLDDKSREFIAYL
                     RDQELDHEKTFMALRDDADMQDLELSQDWEMVSAYLKTIVEGRIFNSEESAIRKAAGA
                     ADLAAVVENAIAFEKDTLLYFHALSDSISNPKALKVLRRIINEEVSHIMRLNDFKKGL
                     A"
     gene            3939..4460
                     /locus_tag="PLB85_02275"
     CDS             3939..4460
                     /locus_tag="PLB85_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU72182.1"
                     /translation="MSTAKKKSKFRRIVYFPLVLLLVLLILVYNPVSVRLMTLSAAVI
                     YGLDPGIFYRLINTESRFRSFAVSPAQAIGLGQVQESTASYVHMSHRRGMLYFPPYNL
                     SISARYLNYLHRRFGNNWTLVLAAYNWGESNVGRRMSGIRIDPEADYRERFSDIRETY
                     GYINKIMPPAKKA"
     gene            4515..5219
                     /locus_tag="PLB85_02280"
     CDS             4515..5219
                     /locus_tag="PLB85_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72183.1"
                     /translation="MLKNQQGSVLFWVLSAVLAIALIAILALSGMFNLDPEKNTDDCT
                     TNMKNIWVAANDYVLETQQDFNGDLNMLRTTTKPGSKQPYLTEEKYCPELQGEKTEYQ
                     VFGKYLYEVIDGETKHYSGILVFCPNIADFPAHVLDKAFYDNMSTTKIQNVMISDLAL
                     IDSAKKSAKQRSEEIQKYLNYWKNTPHKEFNAANSDPALVQWRQSLAPAAPSQSDFFS
                     EENIIEEATPPETETE"
     gene            5311..6585
                     /locus_tag="PLB85_02285"
     CDS             5311..6585
                     /locus_tag="PLB85_02285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOU72184.1"
                     /translation="MRYQEFLDHIYQKYSGNVKLELNRMERLLEEMGNLQAGLRGFHV
                     AGTNGKGSVCATLEALCLAHGHSTGLNTSPHLINYTERFRVNGTELPFESILATYHRL
                     EPLFEKWDASFFEITTAIAFQIFSESKLQSSIIEVGLGGRLDATNLFIPDVAAITTIG
                     LDHVKTLGGSLELIAAEKAGIIKSGVPLVLGRIPASPRRIILNRAKTLNAPVYLPGRD
                     YYSRVTARRVDGIRFDYRFGRHIYRGLVTNLLGEHQAGNVALALTAFFIYANVMGIKV
                     CARKVRHALRNINWRGRMQVLSTAPVIIVDGAHNVQGVKAMLDTLRRVWPRRRFRFLV
                     SILADKNYPEMLRLICLNAEKIYFAQNSSDRAATLAQQAAEAVRNGVDYVEADSVAKA
                     FQIARSELKPDDILVCGGSLFTVGEVLSVWKN"
BASE COUNT         1611 a         1815 c         1838 g         1346 t
ORIGIN      
        1 acgcaaatag tttttgacaa aaaagacaat ggcagaataa gtggcgtaac acactacaat
       61 ccgtatggag acaataatgc acgacgatgt gatccgatat tttctggaac tggtggcaat
      121 cgacagcgaa tcacgtgatg aacgcgccgt gatcgatcgc ctgaaaaccg atctggccgc
      181 tttgggagcg gaggtttttg aagacgacgc ccacaccctc actggaggca acgcgggtaa
      241 cctgcatgcc ctgatcccgg gtcgggcaga ccaaaaaccc atccttttct gcgcccacgc
      301 ggataccgtg aaaccaggcc tcggcgttaa ggcctcgatc accgcgggcc gcatccacac
      361 agacgggacc accgttttgg gtggagacga caagtccggc ctggccgaga tcgtcatggg
      421 aatcaaggat gtggtggaga gcggggaaga acacgcgccc atcgaggttt tggtcacggt
      481 ttcagaagag gtcggccttc tgggggccaa acattttgac aaaagcaagc tgcgcgcggc
      541 ttttggctat gctctggacg cccatcgggt tggtgacctg gtggtgggag cgccggccca
      601 gaactcaatc cggatggtga tcacgggcaa ggaagcgcac gcgggggtgg aacccgaaaa
      661 agggatcaac gccatccgcg tggccgccga agccgttgcc gccatgcctt taggccgcat
      721 cgacaatgaa accacttgca acgtgggcat catctcaggc ggcaaagcga ccaacattgt
      781 tcccaactcc gtggaactga aaggcgaagc acgcagccac aatccccaaa agctggaaca
      841 ggttaccgcc aatatccggc acgctgccga aaacgctgtg cagcggcaca aatacgaatt
      901 tggagcggcc agcctggagt gggaatccaa acaggaatac gcctcattcc ggatttccga
      961 cagcgaagcc gtggtgcagc ttgcgctgga cgccttgaag gatttgggga tcagggctga
     1021 ggtaaccgtt ggcggtggag gcagcgatgc caatataatc aacgcggcgg gcctgccgat
     1081 gatcatctgc ggcaccggca tgaacaaagt gcatacagtt gccgaagaca tcgaaacgga
     1141 agaactgaag cggggagcgg ccttcgtcgc tgctctgatc cgccgctatt caaggagctg
     1201 aaccatgctg gacatcactt tgatcggata tggcaaaatg gggcacatga tcgccagcct
     1261 tgcagaggag catggctgca gggtgcgctc catcatcgac cccggccagg agggttgtga
     1321 aaaggagatc aggccggaaa tcctggctga cgtctgcatc gatttcagcc agccggacat
     1381 ggctttggcc aacatccgcc aggtagccgc ggcgggaaaa cacatggtgg tggggacaac
     1441 aggctggcac aggcatctgg atgaagtgaa ggaactggct gaacgccacg gcatcggcat
     1501 cctctggggc gcgaatttct ccatcggcat gaacctcttt acccgtgtta tccaatacgc
     1561 cacctccatc tgtgacaaat tccccatgta cgatgtctgc ggttgggaaa tgcaccacaa
     1621 ccaaaaggcg gacagccctt ccggaacggc tatccagctt gcagaagcaa tattggagga
     1681 aagcggtgtg aaggatcagg ctgtttacga taagctggac cggcgcatcc tcaagaacga
     1741 gttgcacttc gcaagccttc gcggcggagc gatgccggga acccacagcg tgattttcga
     1801 ttgcgagacc gacagtctgg aattgaccca cagagtgcgt tcccgcgcct gttttgccgc
     1861 cggggcgctg caggcggctc agtggatttc gctgcgcaaa ggtttttaca gtttcaacca
     1921 gatgatggaa gacctgctgt gctgataccc tttggcaagg tccaggccca aggcaacgat
     1981 ttcgtgatgc tgcagcttct gcacacctgg aatacctcac tgccgcttga cgctctggcc
     2041 agggacatct gtgaccgccg caccggagtg ggcgcggacg gcctagtgct gttgctggcg
     2101 gatgatgagg ccgatgccag aatgctgatt tacaacagcg acggcagcca tgctctgatg
     2161 tgcggttccg ccctgcgctg ctgcgcttcc cttctgaaca ggctcacagg taaaacggag
     2221 ctgctcatcg ccaccgacag cggtctcaaa agcgcaactg tgcaggaacg ccagatcagc
     2281 gtcaatctgg gcaaaccggt gctgaaagaa aaggacctgt ccctggaagg gttcacgggt
     2341 tcggtggtgg aggtgggtaa tctgcatttt gtctcgtttc agaacaggct cgaagaccag
     2401 gaattggaaa tcggcccgcg actggagagt cattcccact tccaagaggg ggtgaatgtt
     2461 gaattcgtgc gggttatttc gtgtctggag atcgaaatga aggtttggga acgcggcgcg
     2521 ggggccactc aggcctgcgg caccggcgcc gtggccagcg tttttaccgg tatcggcaaa
     2581 ggcctgctgg acaacgaggt ctgggtgaaa atgcctggcg gcgaggtttg catcacccgg
     2641 ctggaaaacg gggaatttct actcgctggg actgttgaac acactttcag tggagtttac
     2701 caatggagaa tttaggctcg catctgcaaa agctgcgcga agaaaaaggt atcagctacg
     2761 ataaggtttt cgaggatttg cgcctgcggg aagaccagtt acggctgatt gaggaaaacc
     2821 gctattttga gctggggcac ttcggcttcg ccaaggccat ggtgcataaa tacgccggtt
     2881 acctgggggc cgatctggat gaagtgatgg ccgaattcaa ggtaatggtg ccggaaaata
     2941 caaagaaaca gcctgttcgc ggcggcggga gcaaatccag caagatcatg ctctcaccca
     3001 atctgctctg gctgctgggc atcatcctgt ttgtgatcgt cttgggggcc atcctctggt
     3061 atgcccacaa ccagggttgg ctgaaaaccc cggagttttt gaaaccagcc gcggcggata
     3121 ccactgtggt gagcaaaccc cgattgccgc aggaaaagga accgaaggtg gatcccatgc
     3181 gtgaagtgca gaaagaggtt accaaagcgg cagccattcc gtctgtgact cctgcccagc
     3241 ccagccaggg aatccgccag gccgtgcagg acagcaccga ccacatcggc gagttgatgg
     3301 gaccttctca ggtgaatgtg gggctggagt gaacagctcc ccgcagccct gaagcatagc
     3361 ctttccacca ctttcagctt gacaaacaca ctcccggtga gcataatatc tgagaccaca
     3421 aacataaatc caactggagg aataatgcca gctttctcaa tcaacgagat cattgaaatg
     3481 gccgtgcaaa tcgaacgcaa cggctatgcc ttttaccagg aagccactaa aaggaaagac
     3541 ctagacgaca agagcaggga attcatcgct tacttgcgcg accaggaact ggaccacgaa
     3601 aagaccttca tggccctgcg cgacgacgcc gacatgcagg atctggaact ctcccaggac
     3661 tgggaaatgg tttcagccta cctgaagacc atcgtggaag gcaggatctt caacagcgag
     3721 gaatccgcca tccgcaaggc cgccggagct gctgacctgg ctgccgtggt ggaaaacgcc
     3781 atcgcctttg aaaaggacac cctgctttat ttccacgcgc tcagcgacag tatttccaac
     3841 cccaaggctc tcaaggtctt gaggcgcatc atcaacgaag aagtgtcgca cattatgcgg
     3901 cttaacgatt tcaagaaagg cctggcctga tccaagccgt gtccaccgcc aaaaagaaaa
     3961 gcaagttcag gaggattgtc tatttccccc tggtcctgct tttggtgctt ttgatcctgg
     4021 tgtacaaccc cgtttcagtg aggttgatga ccctcagcgc ggctgttatt tatggcctcg
     4081 atccgggcat tttctaccga ctgatcaaca cggaaagccg cttccgcagc ttcgctgtgt
     4141 caccggccca agccatcggc ctggggcagg tgcaggaatc caccgccagt tatgtgcaca
     4201 tgagccacag gcgcggcatg ctctattttc caccctacaa cctcagcatc tcggcccgct
     4261 atctgaacta cctgcacagg cgctttggca acaactggac cttggtgctg gcagcttaca
     4321 attggggcga aagcaatgtt ggcaggcgca tgagcggcat cagaatcgat ccagaagccg
     4381 attacaggga gcgtttctcg gacatccggg aaacctatgg atacatcaac aagattatgc
     4441 cccctgcaaa aaaggcttga cgtgattggg cggtgtaaaa taattagaaa aaccatatct
     4501 ttcaggagtt actcatgttg aaaaaccaac agggaagcgt gcttttctgg gtgttgagcg
     4561 ccgtcctggc catagctctg atcgcgatcc tagccttgtc cgggatgttt aaccttgatc
     4621 ccgaaaagaa caccgacgat tgtaccacaa acatgaaaaa tatctgggtg gcagccaatg
     4681 attatgtttt ggaaacccag caggatttca acggtgacct aaacatgctg cgcaccacta
     4741 ccaaacctgg cagcaaacag ccctacctca ccgaggaaaa atactgccct gaactgcagg
     4801 gcgaaaaaac ggaataccag gtcttcggca aatacctcta cgaagtgatt gacggagaaa
     4861 ccaaacacta ttccggcatc cttgtttttt gccccaacat tgctgacttt cccgcccatg
     4921 ttttggacaa ggccttctac gacaacatga gcaccaccaa aatccagaat gtgatgattt
     4981 ctgatctggc tctgattgac agtgccaaga aaagcgccaa gcaaaggtct gaagagattc
     5041 agaagtatct gaactactgg aaaaacactc cccacaagga attcaacgcc gcgaacagcg
     5101 atccggccct tgtgcagtgg aggcagtcgc tcgctccggc agctcccagc caaagcgact
     5161 ttttttccga agaaaatatc atcgaggaag caactccgcc cgaaacagaa acggaataaa
     5221 tctgaactga cattgacctt tccccgcggc tgaggtggaa gcaccggtcg cggggagttt
     5281 tttccacatc tccaataccg aggaatagct ttgcgatatc aggaattttt ggaccatatc
     5341 tatcaaaagt attccggcaa cgtcaagctg gaattgaaca ggatggagcg tttgctggag
     5401 gaaatgggca atctccaggc tggtctgcgc ggcttccacg tggccggaac caacggcaaa
     5461 ggcagcgttt gcgccactct ggaggctctt tgcctggcgc acgggcacag caccgggctc
     5521 aacacctcgc cccacctgat caattacacc gagcgtttcc gcgttaatgg taccgaactg
     5581 cctttcgaga gcatcctggc tacttaccat cggttagaac ctctgttcga aaagtgggat
     5641 gcctctttct tcgaaataac tactgccatc gcctttcaga ttttcagcga gagcaagctc
     5701 cagagcagca tcatcgaggt tggcctgggc ggaaggctgg atgccaccaa tcttttcatc
     5761 cctgatgtgg cggccatcac caccattggc ctcgaccacg tgaaaaccct gggcggcagc
     5821 ttggaattga tcgcagccga aaaagcgggt atcatcaaat ccggcgtgcc gctggtgctg
     5881 gggcgcattc ccgcttcccc acgacggatc attctcaacc gcgcgaaaac cctgaacgcg
     5941 ccagtctatc ttcccggcag ggattattat agccgcgtca cagcccgccg ggtggacggg
     6001 atccgtttcg attaccgttt tgggcgccac atctaccggg gcctggtcac caacctgctg
     6061 ggtgaacacc aggccggaaa cgtcgccctc gccctcacgg ctttctttat atatgccaat
     6121 gtcatgggta taaaggtctg tgccaggaaa gtgcgccacg ccctgcgaaa catcaactgg
     6181 cgcggccgca tgcaggtgct cagcacggct ccggtgatca ttgttgacgg cgcccacaac
     6241 gttcagggtg tgaaagccat gctggatact ctgcgccgcg tttggccccg tcgccgcttc
     6301 cgttttctgg tctcgatcct ggccgataag aactatcctg aaatgctccg actcatttgc
     6361 ttaaacgccg aaaagatcta cttcgcccaa aactcttccg accgagccgc aaccttggcc
     6421 caacaagcag ctgaggccgt caggaacggg gtggactacg tcgaggcaga ttccgtggcc
     6481 aaagctttcc aaatcgccag atcggagctg aagcctgacg acatactggt ctgtggcggt
     6541 tcgctcttca ccgtgggtga agtgctgagc gtctggaaga attgaatccg ctttctctca
     6601 taaggaccgg
//
LOCUS       DAOOWR010000053         6563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11826_length_6563_cov_3.752766, whole genome shotgun sequence.
ACCESSION   DAOOWR010000053 DAOOWR010000000
VERSION     DAOOWR010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_6563_cov_3.752766"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="PLB85_02290"
     CDS             complement(<1..1416)
                     /locus_tag="PLB85_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOU72185.1"
                     /translation="MIEKMRKYSFVLYHRDYEAFLTELQKLGVLHLIRNTDAKTESLV
                     QNLELIGDYAEASTFLKKLASEAEKGATTLTPKMLLNKITEAREEKERLTRQAETLRK
                     QIRELEPWGRFDYELKKKLEANGIKVDFHTCLKNHFKPQWEEEYAISKISEVNGILYF
                     VVFHTGEKPVLDADTFSFHKHTIKELEDQLKGVEGRIADIDEFLQNTAPTAIESFAEE
                     IGRLSQACEYEDATLQGTSEADDHIRVVGGFIPIRLEPELKNFLERNGVIHFATDATV
                     EDNPPVSLRNGWFARQFEPISKMYMLPFYDEFDLTPFFAPFFMLFFGFCNADIAYGVI
                     FILLALVLRAKAKNPAMKSIMMLVVLFGISSIIMGWVMGSALGYDLKKTALDKYIIIR
                     NNDQIFNFALLLGAIQILFGTIINGFKQARQSGFMYFLAPFGTFLFLLGLSVLGAGLL
                     GADISSTQPYVNYVILAGLALL"
     gene            complement(1413..2006)
                     /locus_tag="PLB85_02295"
     CDS             complement(1413..2006)
                     /locus_tag="PLB85_02295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOU72186.1"
                     /translation="MKFQYNKISQLQLEKQLKVREKALPTLKNKESALRMEVKRARDK
                     AYELDARIKQETAELDQFMKLWAEFDPSLVTVKKVEILTRNIAGTQVPVLGEIDFELR
                     DYDLFSAPSWFLDGIVMVKGLATLQVERDVFWRNMQILEHVRKKTTQKVNLYEKVQIP
                     AFEDAILKIKRFLEDEENLSKAAQKILKGRTAEEEVA"
     gene            complement(2003..3325)
                     /locus_tag="PLB85_02300"
     CDS             complement(2003..3325)
                     /locus_tag="PLB85_02300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOU72187.1"
                     /translation="MASKAFQKIYTKLTQITKATCAVTATGVGYEELATVGGRLAQVV
                     KIMGNTVTLQIFSGTEGIGTDAEVVFFGRPPMLKVSEQLAGRFFNAYGEPIDGGPEIE
                     GEEVEIGGPSVNPVRRKDPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAT
                     VALRTKADKIILGGMGLSNDDYLYYKNTFENAGVIDKIISFVNTNDDPPVERLLVPDM
                     ALTAAEYFAIQKNESVLVLLTDLTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPEGGSITIIAVTTISGGDITHAIPDNTGYITEGQLYLRRDTDIGKVIIDPAR
                     SLSRLKTKVMGKRTRADHPQVMNASVRLNADATNARTKMENGFDLSDYDQRAMDFARD
                     YASQILAIDVNIDTDEMLDTTWALFRKYFTREEVAIRDEFMQLYWDKG"
     gene            complement(3325..5079)
                     /locus_tag="PLB85_02305"
     CDS             complement(3325..5079)
                     /locus_tag="PLB85_02305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOU72188.1"
                     /translation="MTKGTVKGIISNLVHVEVDGPVFQNEICYIDLGGTKLMAEVIKV
                     SGNTAYTQVFESTRGMKPGDAVEFSDRMLEVKLGPGMLSKNYDGLQDDLNKMEGLYLT
                     RGEYTDPLDEDSSWDFEPIVKAGDEVIPGDWLGSVQENWINHKIMVPFKLEGKFRVKS
                     ISGTGKYKLTDTIATITDADGQDTDLNMIQYWPVKLPIRAYQSKPRPFKMMETGVRTI
                     DTLNPIAEGGTGFTPGPFGTGKTVLQHSISQNAEADLIIVAACGERANEVVELFTEFP
                     ELDDPRTGRKLMERTIIICNTSNMPVAAREASVYTAMSIAEYYRSMGLKVLLLADSTS
                     RWAQALREMSNRMEELPGPDAYPMDLPAIISNFYSRAGYVYLNNGQPGSITFIGAVSP
                     AGGNLKEPVTESTKKAARCFYSLSQDRADSKRYPAVDPIDSYSKYLEYDEVIEYLNAN
                     ISPGWVDSVIKAKDILLRGKEAKDQISILGDDGVPLDYHARFWKSELVDRIILVQDGF
                     DPVDKSTPMKRQAYMLNLVLQVCDTPLAFDSFEELQPYYTRLINALRQMNYQPFESAD
                     FKKYESDLESILEERRDN"
     gene            complement(5094..5978)
                     /locus_tag="PLB85_02310"
     CDS             complement(5094..5978)
                     /locus_tag="PLB85_02310"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOU72189.1"
                     /translation="MRTQYYYFVTGLPALSIDDSKCQISTEQFLAEAKNHLTAGDFRL
                     LLLLRLPEDADDLARLMYGKEDEGRPVEECSRIFWKGYLELMKARAAGSSEPLTKDYK
                     AYPDFWHETILAAYSAEEMPSLLDTRHRLLAGSYEYCAKLGNKFLSQWFEFNRDLQNI
                     LTAINGRQHEVDYARWLVGEGELVEKLARSNAADFGLGKGHELFESVSRIWEQNNILH
                     RERGYDILRWKWIDNHNFFNYFNIDRLLGYYAQLRILERWLNLDPGYGREMFQNTMDA
                     LAGSFVFPDIFKVKSIKS"
     gene            complement(5984..>6563)
                     /locus_tag="PLB85_02315"
     CDS             complement(5984..>6563)
                     /locus_tag="PLB85_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72190.1"
                     /translation="LLQRVYDEGVNKAKAEAEAILEKAKAEAENILTNAKTEAEKTIA
                     EAQKEAADIAKNTDSDLKMAAQNTLSAVKQKLTEVLLSEAFDPKLKEAASDADFVKKL
                     ILEMVSVWKESGGTVTISKSLESKLDQHFAQSLSSSAGKGLKVEFSPQMKNGFAISPA
                     DGTYKLSFTDEDFSNLFKSYLRPRSNKILFKQ"
BASE COUNT         1256 a         1706 c         1944 g         1657 t
ORIGIN      
        1 cagcagagcg aggccggcca gaataacata atttacgtag ggctgcgtcg aagagatatc
       61 cgcgcccagt aatccggctc ccagaaccga gagtcccaac aggaaaagga aggtgccgaa
      121 gggggccagg aaatacatga aaccgctttg gcgagcctgt ttgaagccat tgatgatggt
      181 tccgaagaga atctggatgg cacccaggag cagagcgaaa ttgaagatct ggtcattgtt
      241 ccggattatg atgtatttgt ccagagcggt ttttttgagg tcgtaaccca aagctgagcc
      301 catcacccaa cccatgatga tggaggaaat cccgaagagg accaccagca tcataatgct
      361 cttcatggcg gggtttttgg ccttggcgcg gaggactagg gcgaggagga tgaaaatcac
      421 gccataagcg atatcagcat tgcaaaagcc gaaaaagagc ataaagaagg gcgcgaagaa
      481 gggtgtgagg tcaaactcgt catagaacgg aagcatgtac atcttgctga tgggctcgaa
      541 ctggcgggca aaccagccgt tgcgcaagct cacgggggga ttgtcctcga ccgtggcgtc
      601 ggtggcaaag tggatgacgc cattccgctc cagaaagttc ttcagttcag gctccagccg
      661 gatggggatg aaacctccca ccacgcggat gtggtcatcg gcttcgctgg ttccctgcag
      721 ggtggcatcc tcatattcac aggcctggct gagtcggccg atttcttccg cgaagctctc
      781 aatcgcagta ggagctgtgt tttgcaaaaa ctcatcgatg tcggctatcc ggccttccac
      841 gcccttaagc tggtcctcca gctccttgat ggtgtgcttg tggaaactga aggtgtcggc
      901 gtccagaacc ggtttctcac cggtgtggaa aaccacgaag tagaggatgc cgttgacctc
      961 gcttatctta ctgatggcgt attcttcttc ccactgcggc ttaaagtgat tcttgaggca
     1021 agtgtggaaa tcaaccttaa tgccattggc ttccagcttt tttttcagct cgtagtcgaa
     1081 gcggccccag ggctccagtt cgcggatttg cttgcgcagg gtttcggcct ggcgggtcag
     1141 gcgttccttt tcctcgcggg cttccgtgat cttgttgagc agcatttttg gggtgagagt
     1201 ggtggcacct ttttccgctt cggaagccaa tttcttcagg aaggtgctgg cttctgcgta
     1261 gtccccgatc agctcgaggt tttgcaccag cgactcggtt ttggcgtctg tgttgcggat
     1321 cagatggagc acacccagtt tctggagctc ggttagaaag gcctcataat cgcggtgata
     1381 gaggacaaag ctgtatttgc gcatcttttc gatcatgcca cttcctcctc cgcggtgcgg
     1441 cccttgagga ttttctgcgc ggccttggag aggttttcct cgtcctccag gaagcgcttg
     1501 atcttcagga tagcgtcctc gaacgcgggt atctgcactt tttcgtagag attaaccttt
     1561 tgggtggtct tcttgcgcac atgctccagg atctgcatgt tgcgccagaa gacgtcccgc
     1621 tcgacctgca gcgtggccag tcccttcacc atgacgatac cgtccaggaa ccaggaaggt
     1681 gcgctgaaga ggtcgtaatc cctcagctcg aaatcgattt cgcccagcac ggggacctga
     1741 gttccggcga tgttgcgggt gaggatttcc accttcttca ccgtcaccaa ggaggggtca
     1801 aattccgccc agagcttcat gaactgatcc agttcggcgg tttcctgctt gatccgggca
     1861 tccagttcgt aggctttgtc gcgggcgcgt ttcacctcca tgcgcagggc ggattccttg
     1921 tttttcagcg tgggcagggc tttctccctc accttgagct gcttttccag ctgcagctgg
     1981 gaaatcttgt tgtactggaa tttcatccct tgtcccaata aagttgcatg aattcgtctc
     2041 taatcgcgac ctcttcgcgg gtaaagtact tgcggaagag ggcccaggtc gtgtcaagca
     2101 tctcgtcggt gtcgatgtta acgtcgatgg ccagaatctg gctggcgtag tcacgggcga
     2161 aatccattgc ccgctggtcg tagtcggaga ggtcaaagcc gttttccatc ttggttctgg
     2221 cgttggtggc gtctgcgttg aggcgcacgg aggcattcat aacttgggga tggtcggcac
     2281 gggtgcgttt gcccatcacc ttggttttga ggcgggaaag gcttctggcg gggtcgatga
     2341 tcaccttgcc aatgtcggta tctctgcgca ggtagagctg tccttctgtg atgtagcctg
     2401 tgttatcagg aatggcgtgg gtgatgtctc cgccggaaat ggtggtcacg gcgatgatgg
     2461 tgatggagcc gccttcgggg aactgcacag ccttttcata gatcttggcg aggtccgaat
     2521 agagcgaacc cggcatggaa tccttggagg gaatctgatc catgcggttg ctaacaatgg
     2581 agagggcgtc gcaataaagg gtcaggtcgg tgagcaatac cagcacggat tcgtttttct
     2641 gaatggcgaa gtattccgcc gctgtcaggg ccatgtccgg caccaggagg cgttccacgg
     2701 gcggatcgtc gttggtgttc acaaaggaga tgatcttgtc gatcacacca gcgttttcaa
     2761 aggtgttttt gtaatagagg taatcgtcgt ttgagaggcc cattccgccc aggatgatct
     2821 tatcggcttt ggtgcgtagg gcaactgtgg ccatcacctg gttgtagggc tggtccggat
     2881 cggcaaagaa gggaatcttc tggccggtga ccagggtgtt gttgaggtcg attccggcaa
     2941 tgccggtggc gattaattcc gaagggtcct ttctgcgcac agggttcacc gacgggccgc
     3001 cgatctcgac ttcctcaccc tcgatttcag ggccaccgtc gataggctcg ccgtaggcat
     3061 tgaaaaatct tccagccaat tgctcgctca ctttcaacat gggcgggcgc ccgaagaaaa
     3121 ccacttctgc gtcagtgccg atcccctcag tgccggaaaa gatctgcagc gtaacggtgt
     3181 tccccatgat cttcacaacc tgggccagac ggccgccgac cgtggccagt tcttcatagc
     3241 caacgccggt tgcggtcacc gcgcaggtag ctttggtgat ctgggtgagc ttggtatata
     3301 tcttttgaaa tgctttgctg gccatcagtt atccctcctt tcctcaagta tgctctcaag
     3361 atcggattcg tatttcttaa aatcagcgct ttcaaagggc tgatagttca tctgccgcag
     3421 ggcgttgatc agcctggtgt aatagggctg gagttcctca aagctgtcga aggccagagg
     3481 agtgtcacag acctgcagca caaggttcag catataggcc tggcgcttca tgggggtgga
     3541 tttgtccacg gggtcaaagc cgtcctgcac caggatgatg cggtccacca gttcgctttt
     3601 ccagaagcgg gcatgatagt ccagcggtac gccgtcgtcg cccaggatgc tgatttggtc
     3661 tttggcctct tttccgcgca ggaggatgtc cttggccttg atgacgctat ccacccagcc
     3721 gggggagata ttggcgttca gatattcgat aacctcatcg tattcgaggt atttggagta
     3781 ggaatcgatg ggatcgacag ccggatagcg tttgctgtcg gcgcggtcct gagagaggga
     3841 gtagaagcag cgggcagcct ttttggtgga ttccgtgacg ggttccttga ggtttccgcc
     3901 agcgggcgaa actgccccga tgaaggttat tgaaccgggc tggccgttat tgaggtaaac
     3961 gtaaccggcg cgggaataga agtttgagat gatggcggga aggtccatcg ggtaggcatc
     4021 cggcccggga agctcttcca tgcggttgct catctcgcgc agagcctggg cccagcgtga
     4081 ggtggagtcc gccagcagca acactttcag ccccatgctg cggtaatatt cggcgatgga
     4141 catggcggtg taaacagaag cttcgcgcgc tgccaccggc atgttagagg tgttacaaat
     4201 gatgatggtg cgttccatca gcttgcgtcc ggtgcggggg tcgtccagtt cggggaattc
     4261 tgtgaagagc tccaccactt cgttggcgcg ttcgccgcag gctgccacga tgatgaggtc
     4321 ggcctcggcg ttttgggaaa tgctgtgctg gagcactgtc ttgcctgttc cgaagggacc
     4381 cggggtgaag cctgttccgc cctcggcgat ggggttgagc gtgtcgatgg tgcgcacgcc
     4441 ggtttccatc atcttgaagg gtcgcggctt ggactggtag gcgcggatgg gcagtttcac
     4501 cggccagtac tggatcatgt tcaggtcagt atcctggccg tcggcgtcgg ttatcgttgc
     4561 gatggtgtcg gtgagtttgt acttgcccgt gcccgagatg cttttcaccc ggaacttgcc
     4621 ttccagcttg aaagggacca tgatcttgtg gttgatccag ttttcctgaa ctgagcccag
     4681 ccagtcgccg ggtatcactt catcgcccgc tttgacgatg ggttcgaaat cccaactgct
     4741 gtcttcgtcc agcgggtcgg tatattcgcc gcgggtgagg tacaagcctt ccatcttgtt
     4801 caggtcatcc tggaggccgt catagttttt ggagagcatt cccggcccca gcttcacttc
     4861 cagcatgcgg tcggaaaact ccaccgcatc gccgggcttc atcccgcggg tgctttcaaa
     4921 cacctgcgtg taggctgtgt tgccgctcac cttgatcact tcggccatca gcttcgtgcc
     4981 gcccagatcg atgtagcaaa tctcgttctg gaagaccggg ccgtccacct caacgtggac
     5041 caggttgctg attattcctt taacggtgcc tttggtcata acgttttaac cttctatgat
     5101 tttatcgatt ttactttgaa gatatccgga aagacgaagc tgccggccag cgcgtccatc
     5161 gtattctgaa acatttccct gccgtagccg ggatcgaggt tcagccaacg ttccaaaatg
     5221 cgcagttggg cataatatcc cagcaggcgg tcgatgttga aatagttgaa gaaattatgg
     5281 ttatcgatcc atttccagcg caggatgtcg tagcctcgtt cgcggtggag gatgttgttt
     5341 tgctcccaga ttctggagac gctttcaaaa agttcgtggc ctttgccgag gccgaaatcc
     5401 gccgcgttgc tccgggccag cttttccacc agctcgccct ctcccaccag ccagcgggcg
     5461 taatccacct cgtgctggcg gccgttgatg gcggtgagga tgttttgcag gtcgcggttg
     5521 aattcaaacc actggctgag gaatttgttg cccagtttgg cacaatattc gtaggaaccg
     5581 gccagaagcc gatgccgggt gtccagcagg gaaggcattt cctcggcgga ataggcagcc
     5641 agaatggttt cgtgccagaa atcgggatat gctttgtagt cctttgtgag gggctcgctg
     5701 gaaccggccg cgcgggcctt catcagttcc aggtagccct tccagaaaat ccgcgagcat
     5761 tcctcaaccg ggcggccttc gtcttccttg ccatacatga gccgggccag gtcgtcggcg
     5821 tcctccggca ggcggagcaa cagaagcagc ctgaaatcgc ccgcggtgag gtggtttttg
     5881 gcctcggcga ggaactgctc cgtgcttatc tgacacttgc tgtcgtcgat ggacagcgcc
     5941 ggcagacccg tcacgaagta ataatactgt gtgcgcatgg gtcctattgt ttgaagagga
     6001 tcttgttgct gcggggtctc aggtaggatt tgaagaggtt agaaaaatcc tcgtcggtga
     6061 agctgagctt ataggtcccg tccgctggtg agatggcaaa gccgttcttc atctggggtg
     6121 aaaactccac ctttagcccc ttgcccgcgc tgctgctcag gctttgcgcg aaatgctggt
     6181 ccagcttact ttccaggctc ttgctgatgg tcacggtccc gccggattct ttccagacgg
     6241 acaccatttc caggatcagt ttcttcacga aatccgcgtc cgacgcggct tccttcagtt
     6301 tggggtcaaa agcctcgctc agcaggactt ccgtgagctt ttgctttacc gcgctgaggg
     6361 tgttttgcgc ggccattttg aggtcggaat cggtgttttt agcgatgtcc gcagcttctt
     6421 tttgggcttc ggcgatggtc ttttccgcct ccgttttggc gttggtcagg atgttttccg
     6481 cctcggcttt ggctttctcc agaatcgcct cggcttcggc tttcgctttg tttacgccct
     6541 cgtcatacac gcgctgtaaa agg
//
LOCUS       DAOOWR010000054         6542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11878_length_6542_cov_4.040697, whole genome shotgun sequence.
ACCESSION   DAOOWR010000054 DAOOWR010000000
VERSION     DAOOWR010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11878_length_6542_cov_4.040697"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(525..600)
                     /locus_tag="PLB85_02320"
     tRNA            complement(525..600)
                     /locus_tag="PLB85_02320"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:Lys,seq:ctt)
     gene            937..1359
                     /locus_tag="PLB85_02325"
     CDS             937..1359
                     /locus_tag="PLB85_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU72191.1"
                     /translation="MKEEIELRIVKEADPAEILALYRHPGWWQTDDNPQYLTMVQGIV
                     AKSFCFVTARLNGKLVGMGRAISDGCSDAYIQDVVVYSEFRGQGIGGRIVAKLVEFLR
                     AKGIQWIGLISEPGHEDFYERLGFARMQDYTPMLLKME"
     gene            1367..2245
                     /locus_tag="PLB85_02330"
     CDS             1367..2245
                     /locus_tag="PLB85_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOU72192.1"
                     /translation="MDLPELKTLTTVDVPLLRQYLDRWPRQNCDYAVVNLLVWGKIYD
                     NQFTLWRDHLVLYSPKHAYLFFPVGPGLASSDLRELLDAFRTTDPQAKLILIPENWPQ
                     TNPDLENFFTLQEDRDWADYAYAVEKLVNLSGKKMAKKKNLVSQFARSYPDYHVLPVT
                     PEKHDVLLRFTEKWKRERNVDGAYLKTEFQAIQNTLAMWNELPVDGIIICHHNKISAY
                     SIFSPVNPDMITIHFEKYDPDMKGSAQLINWETARHLKGKYTWINREQDMGLKGLRQA
                     KLTYQPDFLVKFISGI"
     gene            complement(2401..3189)
                     /locus_tag="PLB85_02335"
     CDS             complement(2401..3189)
                     /locus_tag="PLB85_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU72193.1"
                     /translation="MFSFLVHALFAALLSSVCLALFSPFVTLRRVSYLGEALSHIAFA
                     GIALALLLGLNLQFTTLIFVIAVALAITWLARRRQLQEANTITIFLSVSMALGIILIS
                     LRRGYSFDLASYLFGNVLLASSADLWMLGILMTVNIGFIAFFYKELFYLSYNPDLAAF
                     YRLPVRMVDRLFMILLAANIVITLRAAGIILVSAQLILPAATAFNLVKRLDHAVVACA
                     FIAILAATGGFALSWWLNLPTSANIVLLEFLLYLFSLLFRPRSA"
     gene            complement(3189..3917)
                     /locus_tag="PLB85_02340"
     CDS             complement(3189..3917)
                     /locus_tag="PLB85_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOU72194.1"
                     /translation="MIRISGLSHSYGGIPVLEDINLELADGEFVAVIGPNGAGKSTLV
                     KLILGLLPLQSGSIEIDGQPHLEWLKAHPLGYLPQHEEFDRKFPATTLDLVLLGLAGE
                     LGVGKRFRASHKDRARQALAKTKIGNLAKKQIGSLSGGELQRVYLARALVSDSDYLIL
                     DEPEASVDLPGVQSFFSLLKDLNEAGKTIITISHDLHTLTEYCSFLVCLNRRLHCHTQ
                     TDLVNAEIIQKTFGDTVRIIEKHY"
     gene            complement(3914..4774)
                     /locus_tag="PLB85_02345"
     CDS             complement(3914..4774)
                     /locus_tag="PLB85_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOU72195.1"
                     /translation="MKRITLLALLMLAIFGCKKAAENQRPLVMASIRPYELLLADLLG
                     PEFEVRCLIPANASPHTFSPGPRDIRALREARLAFSNGLGLETDLEKAFTALGDTHLR
                     AEELLKGVVPESGNPHIWLSPLLMEKLTLRLSERLQKMFPERGEEIANRALDLVARLA
                     ALDERISREREALGPTPLISFHDSFHYFSATYGIDDLGSVQSSPGREPTPRELTRLGE
                     LIRAHGVKAICVEPQMDRKSADVLAREFNLRVIELDPLGFSIQAQTLPDLISENWERM
                     KQAWQTESLP"
     gene            complement(4771..6249)
                     /locus_tag="PLB85_02350"
     CDS             complement(4771..6249)
                     /locus_tag="PLB85_02350"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOU72196.1"
                     /translation="MKIDLLRKPAEYMDTVIIMHGNKGDFQNIEYLPEHIKNAVGIVI
                     KQDEFTFSYKNLKSFPLVHARHRSNIILCGVGNTQDLDCARLRELFALCIRAALKIDA
                     REAFLYPGFSNPVGEVNFGHILAETALLTAYKFDKYLSESKGHSLKAVHLAVNPKTTR
                     HINRGILEGRIFAESTNMARDLVNEPANVIYPDSLAEIAKKAALKYGFSIEVFGQDKL
                     KRLKMEALIAVGKGSKQEPRLILMSWNGNPEPKAKTIGIIGKGLTYDSGGYCIKTPQG
                     MVNMKSDMAGAAAVIGTFAAIATLKLKVNVLGVIAACENMVSGAAYHAGDIIRSMSGK
                     TIEVINTDAEGRLTLIDAIHYAISREHVSQVIDIATLTGAAVGALGNQISAVLTNNEK
                     MLGKLRLASDFTGEMIWQLPTHEPYLDLIKSEVADLKNSSGPMAGTITAGLFIREFVD
                     GKPWLHIDIAGTAHKDKESGINSYGATGVGVRLLTQLLREME"
     gene            complement(6275..>6542)
                     /locus_tag="PLB85_02355"
     CDS             complement(6275..>6542)
                     /locus_tag="PLB85_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72197.1"
                     /translation="EYIDMAFPLPLTIPNIRGSIFAELGTVFDDFDNFQPFDGSKLKD
                     LKLGYGFGPRLDLGYVILALDVTWLTDLETNSKPTFYISLSEDF"
BASE COUNT         1489 a         1714 c         1881 g         1458 t
ORIGIN      
        1 tgtggtagcc aacacgatac aaggcagtat gaccaccagg atcagcaaaa gaatacgcat
       61 ctttttcttc ctttgtttat tctaccgggt aaggcggtaa gggttttcgc tgtctcaaga
      121 aaggtgtaga aagcgccatc atagctgccg ctctatctca ggtaaaagat atagctatcg
      181 tttcccctga ccgaatccgt aacagagcct tggacattgt ttgaagtcat acatccatat
      241 tccttactga taacttggct gaactaagca ttccatacct aaaacccact ttgcctccaa
      301 aggaaattct gtaaagcaaa aaatgttaag cctctctttc tgaaatcatc cctctgacct
      361 tatttaatag aacagttccg ttatacactt tcagcatgac caaactacgg attgcggatc
      421 ggggaaattc agggttcgag tgaaaggttt cgcgatggtt cagccaaaaa aagcggcctg
      481 ccagaaatcc gggccgagat attcttggca agccgctgaa gatatggtac atctcggggg
      541 attcgaaccc ccgacccact gattaagagt cagttgctct accaactgag ctagagatgc
      601 acatggttgt gtcatttttt ttcttaccct caggctgtca agctaaatgc ctcaaaccat
      661 cgccagatcc ctttgccagg gtcgccgcac tcgatgtgac acagccggac gaaatccagc
      721 cagggtgacg ttcatgtgac ccaacgcctt tgattttggc acacttagcc tcgatcatgt
      781 ccagcataag cgcactgttt atccgctctt tatgtgatcc gtgcctgtcg aaaactttcc
      841 cccgccttgt cgcaagcggc ttgacaaaaa cgcatctacc acagattggt attggtgtta
      901 tattgataat ggtaagattt gccctgaagg aaagacatga aagaggaaat agagttaaga
      961 atagtcaaag aggccgatcc ggccgagatt ttggccctct accgccatcc tggctggtgg
     1021 cagacggacg ataaccccca atatctaacc atggtgcaag ggattgtggc caaaagcttt
     1081 tgtttcgtga ccgcccgttt gaacggaaag ctggtcggca tgggccgcgc catcagcgac
     1141 ggctgcagcg atgcttacat ccaggacgtg gtggtttaca gcgaattccg cggacagggc
     1201 attggcggca gaatcgtggc caagctggtc gaattcctcc gtgccaaggg catccagtgg
     1261 atcggtctga tttccgaacc cggccacgag gatttctacg aacggctggg ttttgcccgg
     1321 atgcaggatt acacgcccat gctgcttaaa atggagtgag gagcatttgg atctgcctga
     1381 actgaaaaca cttaccaccg tcgatgtgcc cttgctgcgc cagtatcttg accgctggcc
     1441 gcgccagaac tgcgattacg cggtggtcaa cctgctcgtc tggggcaaga tttacgacaa
     1501 ccaattcacc ctctggcgcg accatttggt gctttacagc cccaaacacg cctatctttt
     1561 cttccccgtg gggcccggcc ttgcctcttc cgacttgcgt gaactgctgg acgctttccg
     1621 caccacagac cctcaagcaa aactcatcct catcccggaa aactggccgc agaccaatcc
     1681 cgacctggag aacttcttca ctctccagga agaccgagac tgggccgatt acgcctatgc
     1741 agtggaaaaa ctggtaaacc tcagcggcaa aaaaatggca aagaagaaaa acctcgtatc
     1801 ccagttcgcg cgctcttatc cagattacca cgtgttgcca gtgaccccgg aaaaacacga
     1861 tgttttgctg cgcttcactg aaaagtggaa acgcgaacgc aacgttgacg gagcctatct
     1921 gaaaacggag ttccaagcca tccagaacac cctggccatg tggaacgagc tgcctgtgga
     1981 cggcatcatt atctgccacc ataacaagat ttcagcctac tctatttttt cacctgtcaa
     2041 cccggacatg atcaccatcc acttcgagaa atacgacccc gacatgaaag gcagcgccca
     2101 actcatcaac tgggaaacag cccgccacct gaagggaaaa tacacctgga ttaaccgaga
     2161 acaggacatg ggcctgaaag gccttcgcca agccaaactg acctaccagc ccgacttcct
     2221 ggtgaaattc atctccggga tatagtttgt aacttcttta ctcccaatct attcctcctt
     2281 tgccccatcc catttgcctc tcacttgcct cccgctgagt tcccgtttgg caagcgggaa
     2341 ctcagcggga agtgaatggg atgtgagtgg gatggggcaa aggaggaata gatttccaag
     2401 tcacgcggag cggggacgga aaagcaggct gaacaggtag agcaggaatt cgaggaggac
     2461 gatgttggcg ctggtgggca gattgagcca ccaggagagc gcgaaaccgc ccgtcgcggc
     2521 gagaatggca ataaaggcgc aggccaccac ggcgtggtcg aggcgtttca ccagattgaa
     2581 ggctgtggcg gcgggcagga tgagctgggc actcaccagg atgatgccgg ccgcgcgcag
     2641 ggttatcacg atattggcgg ccagaaggat catgaatagg cggtccacca tccgcaccgg
     2701 caggcggtaa aacgcggcca ggtcgggatt gtaggaaagg taaaaaagct ctttgtaaaa
     2761 gaaagcgatg aagccgatat tcaccgtcat gaggattccc agcatccaaa ggtccgctga
     2821 cgaggccagc agaacattgc cgaagagata gctggccaga tcgaagctgt agccgcggcg
     2881 gagggaaatc aggatgattc ccagggccat ggagacgctg aggaaaatgg tgatggtgtt
     2941 cgcttcctga agctggcggc gtcgggccag ccaagtgatg gccagggcca cggcgatcac
     3001 aaagatgaga gtggtgaatt gcaggttcag ccccagcaag agcgccagcg caatcccggc
     3061 aaaggcgata tgggaaagcg cttcgcccag ataggacacc cggcgcaggg tcacaaaggg
     3121 cgaaaagagc gccaggcaaa ccgaggagag cagcgcggcg aacagggcat gaaccaaaaa
     3181 tgaaaacatc agtagtgctt ttcgatgatg cgaactgtat cgccgaaggt tttttggatg
     3241 atttcggcgt tcaccagatc ggtctgggtg tggcagtgga gacggcggtt gaggcaaact
     3301 aggaaactgc agtattcggt gagggtgtga aggtcgtggg agatggtgat aatggtttta
     3361 cctgcctcgt tgaggtcctt caggagagaa aagaagctct gaacgccggg caggtccaca
     3421 gaggcctccg gctcgtccag gatcaggtaa tcactgtcgg atacgagcgc ccgggccagg
     3481 taaacgcgtt gaagctctcc gccggagagg cttccgatct gctttttcgc caggttcccg
     3541 attttggttt tagccaaagc ctgccgcgcc cggtccttgt gggaagctcg gaatcttttc
     3601 cccaccccca gttcgccagc caaacccagc agaacgaggt cgagggtggt ggcgggaaat
     3661 ttccggtcga attcctcatg ctggggcaga tagcccaggg ggtgggcttt aagccattcc
     3721 agatgcggct ggccgtcaat ttcgatggag ccgctctgca aaggtaaaag gccgaggatg
     3781 agcttgacca gggtggattt tcccgcgccg ttggggccga tgacggcgac aaattcgccg
     3841 tcggccagtt ccaggttgat gtcctcgagg acgggaatgc cgccgtagct gtgggaaagc
     3901 cctgaaatcc tgatcacggc agggattcgg tctgccaggc ctgcttcatc cgctcccagt
     3961 tctcgctgat aaggtcaggc agcgtttgag cctggatact gaagcccaag ggatcaagtt
     4021 cgatcacgcg aaggttgaat tcccgggcca aaacatcagc ggactttcta tccatctggg
     4081 gttccacgca aatcgcctta actccgtgag cgcggatcaa ctcacccagc cgggtcagtt
     4141 cacggggagt gggttcacgg ccgggagagc tttgcacgct gcccaggtca tcgatgccat
     4201 aagttgcgct gaaatagtga aagctgtcgt gaaagctgat cagcggagtt gggccgagag
     4261 cttcccgttc gcggctgatt ctctcatcca gagcggccag acgggccacc aggtccaggg
     4321 cgcggttggc gatttcctcg ccgcgctcgg ggaacatctt ttgcaggcgt tcgctgaggc
     4381 gtagggtgag tttttccatc agcagcggcg aaagccagat gtggggattt ccgctttcgg
     4441 gaaccacgcc tttcagcagt tcctccgccc ggaggtgggt gtcacccagc gcggtgaaag
     4501 ccttttcaag atcggtttcc aggcccagtc cgttggaaaa ggccagcctg gcttcacgta
     4561 aagccctgat gtcccgggga ccgggactga aggtgtgggg agacgcgtta gccggaatga
     4621 ggcagcgaac ctcgaattcc ggcccaagca ggtcagccag cagaagttcg tagggccgga
     4681 tgctcgccat caccagcgga cgttggtttt cagcggcctt tttacagccg aaaatggcca
     4741 gcatcagcaa ggccagcagg gtaatgcgtt tcattccatt tcacgcaaaa gctgggtgag
     4801 caggcgcacg ccgactccgg tggcgccgta ggaattgata ccgctttcct tgtctttatg
     4861 ggctgtgcct gcgatgtcaa tgtgcagcca gggtttgccg tccacaaatt cgcggatgaa
     4921 gagcccggct gtgatcgttc cggccatagg gccactgctg ttcttgaggt cggcgacctc
     4981 gctctttata aggtccagat atggttcgtg ggtgggcagt tgccagatca tttcgccggt
     5041 gaagtcagag gcgagccgca gcttccccag catcttttcg ttgttggtca gcaccgcgga
     5101 aatctggttg cccaaggcgc cgacagccgc tccggtaagg gtggcgatgt caataacttg
     5161 gctaacgtgt tcgcggctga tggcatagtg gatggcgtcg atcagggtga ggcgcccttc
     5221 cgcgtccgtg ttgatcactt cgatggtttt gccgctcatg ctgcggatga tatcgccggc
     5281 atggtaggca gcgccggaga ccatgttttc acaggcggcg atcacgccca gcacgttcac
     5341 cttgagcttg agggtggcga tggcggcgaa ggtgccgatc actgctgcgg cgccggccat
     5401 gtcgctcttc atattcacca tgccctgggg tgttttgatg cagtagccgc cgctgtcgta
     5461 agtgagccct ttgccgatta tgccgatggt tttggctttg ggttctgggt tgccgttcca
     5521 ggacatcaga atcagcctcg gttcctgctt cgaacctttg cccacggcta taagggcctc
     5581 cattttcagg cgtttcagct tgtcctgccc gaaaacctcg atggaaaagc cgtatttaag
     5641 ggccgctttc ttggcgattt cggccaggga atcgggataa ataacgttgg cgggctcgtt
     5701 caccaggtcg cgggccatgt tagttgattc cgcgaagatg cgtccttcca ggatgccgcg
     5761 gttgatgtgg cgggttgttt tgggattcac ggccagatgc accgctttca ggctatggcc
     5821 cttgctttcg ctgagatatt tgtcgaactt gtaagctgtc agtagcgcgg tttcagccaa
     5881 gatatggccg aaattcacct cacccacggg attgctgaaa cctggataga gaaaggcctc
     5941 gcgagcgtcg attttgaggg cggcccggat acagagggcg aaaagttcgc gcaacctggc
     6001 gcagtcgagg tcctgggtat tgcccacgcc gcaaaggatt atgttgctgc ggtggcgggc
     6061 gtgcaccagg ggaaagctct tcaggttttt gtagctgaaa gtgaattcat cctgcttgat
     6121 gacgatgccc acggcattct tgatgtgttc gggcaaatac tcaatgttct ggaaatcacc
     6181 tttgtttccg tgcatgatga tcactgtgtc catatactcc gccggtttgc gcagcaggtc
     6241 gatcttcatt gttatctcct atttatgttt gatgttaaaa gtcttcgctg aggctgatgt
     6301 agaaggtggg cttggagttg gtttccaggt ctgtgagcca ggtaacgtcc aaagccagga
     6361 taacatagcc caggtccagg cgcggaccga agccgtagcc aagcttgagg tccttcagct
     6421 tgctgccgtc aaaaggctgg aagttgtcaa aatcgtcaaa caccgtgccc agttctgcga
     6481 aaatgctgcc ccggatgttg gggatggtca ggggcagggg aaaggccata tctatgtatt
     6541 cg
//
LOCUS       DAOOWR010000055         6533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11898_length_6533_cov_3.828188, whole genome shotgun sequence.
ACCESSION   DAOOWR010000055 DAOOWR010000000
VERSION     DAOOWR010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11898_length_6533_cov_3.828188"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..767)
                     /locus_tag="PLB85_02360"
     CDS             complement(291..767)
                     /locus_tag="PLB85_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOU72198.1"
                     /translation="MNERPSWQQYFMQMAILASSRSTCLRRQVGAVLVKENQILSTGY
                     NGSPKGVRHCSETGCLRLQNKVPSGQQHELCRGLHAEQNAIIQAGLNGSSARGATLYC
                     THQPCSICARMIINAEIVTIYISNTYPDALAEQLLAEAGVELIHFDMESGKMKKLL"
     gene            1144..2895
                     /locus_tag="PLB85_02365"
     CDS             1144..2895
                     /locus_tag="PLB85_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72199.1"
                     /translation="MRTFDAVLPYLKKSYGRIAGGIVMLILVDIVQLITPRVMQYAID
                     SIQERRITSTGLIWVALIIIGLALAVMILRYFWRVLIIGNAFRIEKHLRQDFYNHLLR
                     LSQNFFNRSKIGDLMAYATNDLNAVRMLFGMGLIAGMDIVLMTIASFSFMGSIDWRLT
                     GLAVLPMPILTITITIFGKKMHKSFFRVQKSFATLSGAIQESISGIRIVKAFGQEKSE
                     LEKVDEVSWQFVQENIRMAKIAGFFHPFQSLVISLSMVITLFFGGRAAIRGDITIGQF
                     IAFFQYLGMLVWPMIAIGWIVDMYQRGTASLKRLNEIFQTAPEIDDSEADPAIKSLQG
                     NIEIRDLSFRYGENMPLIFDGISTSVSDGKTLAVVGPTGCGKTTLIELLVRIYEPPKG
                     SILIDGHPLHRIPLNVLRRDMVLVPQDIFLFSDSISNNIRLGSPETSTEQVLEAARLA
                     QIHDEVMEFENKFDTVIGERGVTLSGGQKQRVAIARALLTNPEILILDDALSAVDTKT
                     ERFILEKLIETRKGKTTIIIAHRISSIQHADRIIVLGDGKITESGTHDELVALGGLYH
                     DLYEKQRIRARLEGEEL"
     gene            2892..5048
                     /locus_tag="PLB85_02370"
     CDS             2892..5048
                     /locus_tag="PLB85_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72200.1"
                     /translation="MMRGGRGGGFAEDDLKNRVFDKHLYRRMLRYLLPYMKWVVISFL
                     ILMVVAGAELVQPLIQQHAIDDYIVSDKNIAIFNSEAEADAFLAKYELLKLKKLSHSG
                     KYFLVIPSREMNKINRQDVDDLNRRNILAGAKVYLIKDKASNVAILNKYLPEISAPQG
                     GKGGLQGWFRLGNGQIALEREQLNKIPGKERFQVRSEAAHSLIWLALAFFGISLMRFV
                     AGYSQMVITTTFSQKAMNDMRHDVFAHLQRMPVQYFDKNPVGRLVTRVTNDIRAIDEM
                     LSSGVITLFQDVLMIIAIVVLMLFLDWELALISFAILPGVIWVIREFRKRTRVLYREV
                     RKQLAALNATLAEHISGQKIIQLFNQYFHKRDEFSRINREYFNTSLKQMKLFAFFRPI
                     IHVSSQIAVALIIWYGGGQILRNAITIGLLMAFTQYITKLFQPINDFSEKFNVLQGAL
                     AGAERIFDLMDQEPEDYRDDLVCDMKLKGEIEFRNVWLAYNPGEWVLKDVSFKIKPGK
                     KIALVGHTGSGKTSIVNLVLGMYPFQKGEILLDGKPLSSYALADLRRNVGIVQQDVFI
                     FSGNIRDNIALNNTELSREQIIQVAKYVNADKFISKLPEGYDEPVMERGATLSTGQRQ
                     LIAFARVLAYDPSIFILDEATSNIDTETELLIQDALAKLIVNRSSIIIAHRLSTIQHA
                     DRIIVLHKGEIVEEGSHFDLLDQKGLYYDLYRLQYT"
     gene            5217..>6533
                     /locus_tag="PLB85_02375"
     CDS             5217..>6533
                     /locus_tag="PLB85_02375"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72201.1"
                     /translation="MKKRWLVILALTIVAAMFANGNRYEVVAWQETFEDGAAGWTHFD
                     GSVPPNNWHIHNYGGAQGDVWWMGDPDLASGSNIGGYYDHQYLVLDTPARTIASGNAT
                     LTFKMATGLESVGGTGEYNGWDSFNVRVSTNNGTTWTVLTPTTPVYDFTSSYAFGFEH
                     GEGVGIPGWGGVHTAWTPVSVDLSAYVGQSVKIRFAFASDPAYSTGDNLALFGVMVDD
                     ISFGGYTNNGTDDGQMTMSSLVPVGGDIWSIGTDPTAPSPTHMMQCQNAQGSYNPNMM
                     NYLVSPPIQLPNDGDIRVDFMIKGYFDDLDAFPQCDYFGWEISPNNGLSWYAMSNPYN
                     DPTGTNYVYSDAPDVWASMVNSYSLNGLISDYAGQTIMLRWFFKSDEDTPIGTGIMID
                     DVTVYNDIFIAPPANLAATVDGNNITLTWEEPGSGGGGGEEGWLHYD"
BASE COUNT         1577 a         1799 c         1725 g         1432 t
ORIGIN      
        1 caaacgggaa ctctgcggga agtgaatggg atgtgaaagg gatggggcaa aggacgaagg
       61 ccgcaggata cctcctggat tggcattcag gacctgtcgt aaacctctaa gtataatggg
      121 taataatagt tgaatattta ctttggatgg tgaaaagcaa gttacaaatc agctgatgca
      181 gcggagaaac tgcctttagg ggccagccta agcctttaac aagatgaggg ttgcgactcc
      241 gagaagaacg gaggtgacga cgaggtcgcg gatattgaaa tgcttgaacg ttagagcagc
      301 ttcttcattt tgccgctctc catgtcgaaa tggatcaatt ccaccccggc ttccgccaac
      361 aactgctccg caagggcgtc tggataggtg ttggagatgt aaatggtaac gatttcggcg
      421 ttgatgatca tcctggcgca gatgctgcag ggctgatgtg tgcagtaaag cgtggctccg
      481 cgggcgctgg agccgttcag gccggcctgt atgatggcgt tctgttcggc gtgtaggccg
      541 cggcaaagct catgctgctg gccggaggga accttgttct ggaggcgcag gcagcctgtt
      601 tcagaacagt gacgcacgcc ttttgggctg ccgttgtagc cggtggaaag gatttggttt
      661 tccttcacca gcacagcccc aacctgcctt cgcaaacagg tgctgcgcga ggaggccagg
      721 atcgccattt gcatgaaata ttgctgccat gagggtcgtt cgttcatcgt catatccaaa
      781 gaggggattt gcgatcaagc ttgtcggggg cggctttctg tcaagtgcca aatccagagg
      841 acgctctgaa tgtgaaaaaa cttgccagaa cagatagtat tagaaaggta ataagcaaat
      901 aactgagcat tttcaaattt catgctgggg atttttcgga gaaatgtgga taactgccac
      961 gagtcaattc ctgatagtta attgcttggc attacgcaaa ggtctttttt atattgacaa
     1021 aatgggactg cgggggagtt tggcgcgcac tatccggtgt tgtcaacgga aagtggaaag
     1081 ctctccaaaa cagaacagta gggggtttcc ggtttgcgtt gagaatatca atagggagaa
     1141 ctaatgcgca cttttgacgc cgttcttcca tatctgaaga agagttatgg aagaatagcc
     1201 gggggcatag tgatgctgat cctggtggat attgtgcagc ttatcacccc ccgggtgatg
     1261 caatacgcca tcgacagcat ccaggagcgg agaatcacct ccacagggct gatttgggtg
     1321 gcgctaatca tcatcgggct ggctttggca gtgatgatac tgcgctattt ctggagggtt
     1381 ttgatcatcg gcaatgcctt caggatcgaa aagcatctgc ggcaggattt ttacaaccat
     1441 ctactgaggc tttctcagaa cttttttaac cgcagtaaga tcggcgacct gatggcctat
     1501 gcaaccaacg acctgaacgc cgtgaggatg ctctttggca tggggcttat cgcgggaatg
     1561 gacatcgtgc tgatgacgat cgccagcttc agcttcatgg gctcgataga ctggcgcctg
     1621 accggtttgg cggtgctgcc gatgcctatc ctgaccatca ccatcacgat tttcggcaag
     1681 aagatgcaca aaagcttctt tcgggtgcag aaaagcttcg ccacgctctc cggggccatc
     1741 caggaaagca tctccgggat tcgcatcgtg aaagcctttg gccaggaaaa atccgagctg
     1801 gagaaagtgg atgaggtttc ctggcagttt gtgcaggaaa atatccgcat ggctaaaatc
     1861 gccggctttt ttcacccctt ccagagcctg gtgatcagtc tttcgatggt gataaccctt
     1921 ttctttggcg gtcgcgcagc catccgcggc gacatcacca tcgggcagtt catagccttc
     1981 ttccagtatc tgggaatgct ggtctggccg atgatagcca tcggctggat cgttgacatg
     2041 taccagcgtg gcaccgcctc gctgaagcgg ctgaacgaga ttttccagac cgccccggaa
     2101 atcgacgaca gcgaggctga ccctgccatc aaatccctgc agggcaacat cgaaatccgt
     2161 gacctcagct tccgctacgg tgagaatatg ccccttatct tcgacggcat cagcacctcg
     2221 gtctccgacg gcaaaaccct ggccgtcgtg ggccccacag gctgcggcaa aactacttta
     2281 atagagctgc tggtgcgcat ctacgaacct cccaaaggca gcatcctcat tgacgggcac
     2341 ccgctgcacc ggattccgct gaatgtgctg aggcgcgaca tggtgctggt cccccaggac
     2401 atcttcctct tttcggacag catctccaac aacatccggc ttgggagccc ggaaacaagc
     2461 actgaacagg ttttggaagc agccaggctg gcccagatac acgatgaggt catggaattt
     2521 gaaaacaaat tcgacacagt gatcggtgaa cgcggcgtaa ccctctccgg aggccagaaa
     2581 caacgcgtcg ccatcgccag ggcgctgctt accaatcccg aaatcctgat cctggacgac
     2641 gccctctccg ctgtggacac caaaaccgag cgctttatcc tggaaaaact tatcgagacc
     2701 cgcaaaggca agaccaccat catcatcgcc caccgcatct cttcaatcca gcatgccgac
     2761 cgcatcatcg tcctaggtga cggcaaaatc accgaaagcg gcactcatga cgaactggta
     2821 gccctgggcg gcctgtatca tgacctctac gaaaaacaac gcatccgggc ccgtctggaa
     2881 ggggaggagt tatgatgcgc ggcggacgcg gcggcggttt cgcggaagac gacctcaaga
     2941 acagggtttt cgacaaacac ctttacagga ggatgttgcg ctacctgttg ccatacatga
     3001 aatgggtggt tatctcgttc ctcatcctga tggtggtggc cggggcagaa ctggtgcagc
     3061 cccttatcca gcaacacgcc atcgacgatt atatcgtttc cgacaaaaac atagccattt
     3121 tcaacagcga agctgaggcg gacgcctttc tggccaaata cgaactactg aaactgaaga
     3181 aactctcaca cagcggcaaa tacttcctgg tgataccaag cagggaaatg aacaaaatca
     3241 accgccagga tgtggatgac ctcaaccggc gcaacatcct tgccggggca aaggtgtatc
     3301 tgatcaagga caaagcctcc aacgtggcca tcctgaataa atatctgccg gaaatcagcg
     3361 ctccgcaagg cggcaaagga ggattgcaag gctggttcag gctgggaaac ggccaaatcg
     3421 ccctggagcg cgagcagctt aacaaaattc cgggaaagga acgcttccaa gtccgcagtg
     3481 aagcggctca cagcctcatc tggctggcac tggccttctt tggaatcagc ctaatgcgct
     3541 ttgtggcggg gtattcccag atggttatca ccaccacctt ttcgcagaaa gcgatgaacg
     3601 acatgcgcca tgacgttttt gcccacctgc agcgcatgcc ggtgcagtat ttcgacaaaa
     3661 accccgtcgg acgcctggtc acccgcgtca ccaacgacat ccgcgccatc gacgagatgc
     3721 tctcttcggg ggtgatcacg ctgttccagg acgtgctgat gatcattgcc atcgttgtgc
     3781 taatgctgtt tctggactgg gaactggcgc tgatcagctt cgccatcctg cccggggtga
     3841 tctgggtaat ccgtgaattc cgcaaacgca cccgggtgct ttaccgcgag gtgcgcaagc
     3901 aattggcagc gttgaacgcc accctggcag aacatatcag cgggcagaaa atcattcagc
     3961 tcttcaacca gtatttccac aagcgggatg agttttcacg catcaaccgg gaatacttca
     4021 acacttcgct gaagcagatg aagctcttcg ccttcttccg gcctatcatc cacgtgagca
     4081 gccagatcgc ggtggccctg atcatctggt acggcggcgg gcaaatcctg cgaaacgcca
     4141 tcaccatcgg cctgctgatg gccttcacgc agtacatcac caagctgttt cagcccataa
     4201 acgatttctc ggaaaaattc aacgtgctgc agggtgcttt ggcaggcgca gagcgcatct
     4261 ttgacctcat ggaccaggaa ccggaggatt accgcgacga cttggtctgc gacatgaagc
     4321 tgaaaggcga aatcgagttc cgaaacgtct ggctggccta caatccgggc gaatgggtgc
     4381 tgaaagacgt gagcttcaag atcaagcccg gcaaaaagat agcgctggtg ggccacaccg
     4441 gtagcggcaa gacatccatc gtaaaccttg tcttgggcat gtaccccttc cagaaaggag
     4501 aaatcctgct ggatgggaag ccgctctcct cctacgcttt ggcggacctg cgcagaaacg
     4561 tgggcatcgt tcagcaggac gtgttcatct tcagcggaaa catcagggac aacatagccc
     4621 taaacaatac cgagctcagc cgcgagcaaa tcatccaggt ggccaagtat gtgaacgcgg
     4681 ataaattcat cagcaagctg cccgagggtt atgacgaacc ggttatggaa cgtggcgcca
     4741 ccctctccac cgggcagcgg cagttaatag ctttcgcacg cgtgctggct tacgatccct
     4801 ccatctttat cctggacgag gcgacctcca atatcgacac cgaaaccgaa ctgctgatcc
     4861 aggacgcctt ggccaaactg atcgtcaacc gctcctcgat catcatcgcc caccgccttt
     4921 ccaccatcca gcacgccgac cggatcatcg ttctgcacaa aggcgaaatc gtggaggaag
     4981 gctcacattt tgatcttttg gaccaaaaag gcctctacta tgacctctac cggctgcaat
     5041 acacctaaag cgtttcataa cgccatatct gcaaatgggt tgggttgata atatgttcct
     5101 gccaatggaa tgaaacttgc ttttatggta acatcaatga aaaaactgat tgacaggaaa
     5161 cgcagatcag ttgttattgc atagataaac tatcccttga ttcataagga ggatcaatga
     5221 aaaaaagatg gcttgtaatt ctggccctga caattgtcgc agccatgttt gccaacggca
     5281 accgctacga agtggtggca tggcaggaaa cctttgaaga cggtgctgcc ggatggaccc
     5341 actttgatgg tagcgtccct cccaacaact ggcatatcca taactacggc ggagcacagg
     5401 gcgacgtctg gtggatgggc gaccccgacc ttgcctctgg atccaacatc ggcggttatt
     5461 atgaccacca gtatctggtt ctggacaccc cggcccgcac tatcgcgtcc ggcaatgcca
     5521 ccctcacctt caagatggcc actggcctcg aatccgtggg cggaaccggc gaatacaacg
     5581 gttgggactc cttcaacgtt cgcgtctcca ccaacaacgg caccacgtgg accgtcctga
     5641 cccctacaac cccagtttat gacttcacct cttcctacgc cttcggtttt gagcatggcg
     5701 aaggtgtcgg aattcccggc tggggtggcg tgcacactgc ctggacccct gttagcgtgg
     5761 atctttcagc ctacgttggc cagagcgtca agatccgttt cgcctttgcc tccgacccgg
     5821 cttattcgac cggcgacaac cttgccctct tcggcgtgat ggttgacgac atctccttcg
     5881 gcggctatac caataacggc accgacgacg gccaaatgac catgagcagt cttgtgcccg
     5941 tgggcggcga tatctggtcc attggcacag accccaccgc tccgtcaccc acccacatga
     6001 tgcaatgcca aaacgcccag ggcagctaca atcccaacat gatgaactat ctggtgagcc
     6061 cgcccatcca gcttcccaac gatggcgaca tccgcgtgga tttcatgatc aaaggttact
     6121 tcgatgatct ggacgcattc ccgcaatgtg actatttcgg ctgggaaatt tcccccaaca
     6181 acggccttag ctggtatgcc atgagtaatc cctacaacga tcccaccggt accaactatg
     6241 tctattccga cgctccggat gtctgggcat ccatggtcaa cagttattca ttgaacggtc
     6301 tcatctccga ttatgccgga cagaccatca tgctgcgctg gttcttcaaa tccgatgaag
     6361 atactcccat tggcaccggc atcatgatcg acgacgtgac cgtttacaac gacatattca
     6421 tcgcgcctcc ggccaatctg gctgccacgg ttgatggcaa caatatcacc ctcacttggg
     6481 aagaacccgg ttccggcggt ggcggcggcg aagaaggctg gctgcactat gac
//
LOCUS       DAOOWR010000056         6503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_11952_length_6503_cov_3.375310, whole genome shotgun sequence.
ACCESSION   DAOOWR010000056 DAOOWR010000000
VERSION     DAOOWR010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11952_length_6503_cov_3.375310"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLB85_02380"
     CDS             <1..846
                     /locus_tag="PLB85_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU72202.1"
                     /translation="EWIESLNLTEKAKQIAEEALIKVKAEPLGPEARRTLILDPVHLG
                     LTMHESVGHATELDRVLGWEADYAGISFATPEKLKNYRYGSEIVNFVGDNTLNEGLAT
                     LGFDDDGVPGQKWHIIKDGILNEYGSTRDTAMEIGLEYSRGCNRATYYSDQPINRIPN
                     LYLLPGTRELSPAELIADTEDGIYIQGQGSFSIDQHRVNFQFGGDFFWEIKNGKLFRP
                     VKKVIYKSCNPEFWNSCDAICDERYWRPFGVINCGKGQPSQSARMTHGAATARFRNIR
                     VGGSQ"
     gene            843..2123
                     /locus_tag="PLB85_02385"
     CDS             843..2123
                     /locus_tag="PLB85_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU72203.1"
                     /translation="MNKQFEQIEKYIRSHCQADDYSLSISASDNHQTRFAQNGITQHI
                     AGPRLDITLEVAFGQRTGSCSVNQDDEATLAYLVKTAEDIARLNRPDPEYLPSTSAQT
                     LPDVNNSDPSTRELEPAEMVEIVQQTIDKAKAYGATVSGMTEKHVYEYCLFTKNGFAG
                     SDSLTQFGHSMTLKKENVETKVSHEAKDFASFSLEQEFGRLLSQAESLLTMKDFDACK
                     IPVILRPGAIGELLLYMGWMMNRRQSDEGFTPYTGQLGQPFFGEKFSLRSTLKEPRLY
                     ARPFDHEGIVSREISWVENGILQNMPTNRHWAKEKNAQPQMLFNYFVPGGEASEEEMM
                     KLVPRGLIINRFWYIRTVDAKAGEFTGMTRDGVLYFEDGKVKNAVNNLRFNEIPHDAT
                     RRILALGKQELPFLYAYIPTMLIDNFNFVDKTTF"
     gene            2263..2439
                     /locus_tag="PLB85_02390"
     CDS             2263..2439
                     /locus_tag="PLB85_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOU72204.1"
                     /translation="MPDKTLVCKDCSNEFVFTEGEQEFYKEKGLANEPQRCPECRKAK
                     KQMYNRNRFQRDPH"
     gene            complement(3559..3867)
                     /locus_tag="PLB85_02395"
     CDS             complement(3559..3867)
                     /locus_tag="PLB85_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72205.1"
                     /translation="MKTRFLLLATLLILLLAACGTNQGAAGSGSASEYGYLNLSGSMK
                     NREAWVDGVQMGIDPEADNHRVRLTIGIHKLEFRSANRILRADEILIEAGKILEIPIP
                     "
     gene            4104..>6503
                     /gene="secA"
                     /locus_tag="PLB85_02400"
     CDS             4104..>6503
                     /gene="secA"
                     /locus_tag="PLB85_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU72206.1"
                     /translation="MLEKLLRKIFGDKSTRDLNLYSPQVEAINLIFEGLHQYDDDQLR
                     ERVADIRGEIAEKLRDLRETLDDLERRFREEAEESERNRIDNRIDETRKQLKALTKST
                     LDDYLPEVFALVKETCRRLVDQEFEVRGHMLKWNMVPFDVQLIGGMALHDGKIAEMAT
                     GEGKTLVATLPLFLNALVGRGVHLVTVNDYLASRDAEWMSPVFNFHGMKVGCITTGMS
                     FEERKEAYLCDVTYGMNSEFGFDYLRDNMAVSPQQLVQRDFYYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYPELRPQINQLVQSQNSMVQRVLSEIREDIREDYPAADMDRLA
                     TNLLIVQRAAPRNKAFAKLMQESWLKKLVNDMEGIYLRDKRMHELDEKLFYVVEERQN
                     SVDLCEKGRELLSRHEKNLFVVESLDDVLNRVDEDESLSEADRIKRKTLETNRFMDKS
                     EKLHNINQLLKAYTLFENDKEYVVIDNKVIIVDEFTGRQMPGRRFSDGLHQALEAKEN
                     VTIEAGTQTFATITLQNYFRMFERLSGMTGTAVTEEAEFIEIYNLPVMTIPTNVPVTR
                     VDHEDVIYMTKNEKYQALMDEIIYWHELQKPVLVGTVSVEVSETISRLLRRRNIPHSV
                     LNARQHQREAEIITSAGKPGSVTIATNMAGRGTDIKLGPGVVTQSTENYRGIDSAVTD
                     GFPYGQPLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLLRIFGAD
                     RISSIMDRIGMEEGQPIEHRLISKAIENAQKRVEGQNFDIRKHLLEYDDVMNRQRQVI
                     YE"
BASE COUNT         1633 a         1868 c         1754 g         1248 t
ORIGIN      
        1 gaatggatcg aaagcctgaa cctgacggaa aaagcgaagc agatcgccga agaggccctg
       61 atcaaggtga aagccgaacc tttgggcccg gaagcacggc gcacgctgat cctggacccc
      121 gtccacctgg gcctcaccat gcacgaaagc gtggggcacg ccacagaact ggaccgcgtt
      181 ttgggttggg aagctgacta tgccggcatc tccttcgcca ctccggaaaa gctgaaaaac
      241 taccgctacg gcagcgagat cgtaaatttc gtgggcgaca acaccctcaa cgaagggctg
      301 gccacgcttg gatttgacga cgacggcgtc cccggccaga aatggcacat catcaaagac
      361 ggcatcctga acgaatatgg cagcacccgc gacaccgcaa tggaaatagg tttggagtat
      421 tcccgcggct gcaaccgcgc cacttattat tctgatcagc ccatcaaccg catccccaac
      481 ctctatctgc tgcccggcac cagggaacta agcccggccg agctgatcgc cgataccgag
      541 gacggaattt acatccaggg ccagggaagc ttttccatcg accagcaccg ggtcaatttt
      601 cagttcggcg gcgatttctt ttgggaaatc aagaacggca agctgttccg tcccgtcaag
      661 aaagtgattt acaaatcctg caacccggaa ttctggaaca gttgtgacgc catctgcgat
      721 gaacgctact ggcggccttt cggagttatc aactgcggca agggccagcc cagccagtcg
      781 gcgcgcatga cgcacggcgc cgcgaccgcc agattccgca acatccgcgt gggaggttca
      841 caatgaacaa gcagttcgaa caaatcgaaa aatacatccg cagccactgc caggctgacg
      901 attacagcct cagcatcagc gccagcgaca accaccagac ccgcttcgcc cagaacggca
      961 tcacccagca catcgccggc cccaggctgg acattactct ggaggtcgcc ttcggccagc
     1021 ggacaggttc ctgcagcgtg aaccaggacg atgaggccac gcttgcctat ctggttaaaa
     1081 ccgctgaaga catcgccagg ctcaaccgcc ccgacccgga atacctgccc agcacgagcg
     1141 cgcaaacgct tccggatgtg aacaacagcg atccctccac gcgtgaactg gagccggcag
     1201 agatggtgga aatcgtgcag cagaccatcg acaaggccaa agcctacgga gctacggttt
     1261 ccggaatgac cgagaaacac gtctatgaat actgcctctt taccaaaaac ggctttgcgg
     1321 gcagcgattc cttaacccaa ttcggccatt ccatgaccct caaaaaggaa aacgtggaaa
     1381 ccaaggtttc ccacgaggcc aaggattttg cctcgttttc gcttgaacag gagttcgggc
     1441 gtctgctctc ccaggcggaa agcctgctca cgatgaaaga tttcgatgcc tgcaaaatcc
     1501 cggttatcct gcgccccggc gcgatcggcg agctgctgct ctatatgggc tggatgatga
     1561 accggcgcca gagcgatgag ggtttcaccc cttacaccgg ccaactgggg cagcctttct
     1621 tcggggaaaa attctcgctg cgctcaaccc tgaaggagcc ccggctctac gcccgtccct
     1681 ttgaccatga aggcatcgtc agcagggaaa tcagctgggt ggagaacgga atcctgcaaa
     1741 acatgcccac gaatcggcac tgggccaaag aaaagaacgc ccagccgcaa atgctcttca
     1801 actatttcgt ccccggcggc gaagccagcg aggaagagat gatgaagctg gtgccgaggg
     1861 gcttgatcat caaccgcttc tggtatatcc gcaccgtgga tgccaaagcg ggggaattca
     1921 cgggaatgac ccgcgacgga gtgctctact tcgaagacgg gaaggtgaaa aacgccgtga
     1981 acaacctgcg ctttaacgaa atcccgcacg acgccacgcg acgcatcctg gccctgggca
     2041 agcaggaact gccattcctt tacgcctaca tacccaccat gctgatcgat aacttcaact
     2101 ttgtggacaa aaccactttc tgaaaaataa agattgacag aatgcagacc ctgaagattt
     2161 tacgcatcaa acagggatta acattccatg agcctattcc ttttgttctg ggagagcctc
     2221 aaacccgaat gtcagacgtc ccgaacaaaa aggagaaagc ccatgccaga caagacattg
     2281 gtttgtaaag attgctcgaa cgaattcgtg ttcaccgaag gcgaacagga attttacaag
     2341 gaaaagggtt tggccaacga gccccagcgc tgcccggaat gccgcaaggc caaaaagcag
     2401 atgtacaacc gcaacagatt tcagcgcgac ccccattgag ttaagctgat tccaagcata
     2461 aaacgccgtc cttcatgggc ggcgattttt ttgccaaaaa gaaggctccc tttcaaaccg
     2521 ggtggataaa acccggtgtt tccagcccgg acggcggcag taatcaagcc ccgctggatt
     2581 gcgaatcgat gctgattggc cgttaaggcc ttttcctatg tcccggttgt gcctcccgca
     2641 caatagccgc acttgatgcg aggattatac gcggtgcgtg ggagggggat ggaaacgggc
     2701 gttcagggcc tggctggcag gaaactgaca ggactttgct gtgggtaagt atttatggct
     2761 cttcaccttg ttattagcct taacaacata catacccgtc cacaaaaatc cacgtcattc
     2821 cgggcttgcc ccggaatcca gtcctctttc tgtcatggat cccggatcaa tgtcacctcg
     2881 caagctcggt gacgtccggg atgacgtatg ttcggctctc cggccagtgt ctttacgcgt
     2941 cattctgggc tcgacccgga atccagactg gggtggaata gcctataaac caccacccga
     3001 ccccaaaccc acgtcattcc gggcttgacc cggaatccag tgtacatccc ttcatggatc
     3061 ccggatcaat gtcacctcac gagctcggtg acgtccggga tgacgtatgt tcgatatctt
     3121 tgagaaaagg aaaatggctg tacagcaacc gcctgtacaa aggtagcggc gctcgccgaa
     3181 gcgccgaaac tcccaaatcc acgccattcc gggctcgacc cggaatccag aactggctga
     3241 atagcctata aaccaccacc cagcctccat cccacgtcat tccgggcttg acccggaatc
     3301 cagtccatat cccgttatgg atcctggatc aaagtcacct cgcgggctcg gtgacgtccg
     3361 ggatgacgta tcctggagcc tggcgtccag cgaacgcagg gagcatggac ggcaggaaat
     3421 gttgctgtcg tcgatgctcg ttcctcgctc tacgccagcc caccgccccc tcgctatcgt
     3481 tatgcagccc tacggggtcg atcaactgga tcacccacgc gatttgaaag cgagccactt
     3541 agtttaagcg caaccctctc agggaatcgg gatttcgagg atcttcccgg cctcgatcag
     3601 aatctcatcg gcccgtaaaa tgcggtttgc ggagcggaat tccagcttgt ggatcccgat
     3661 tgtgaggcga acgcggtggt tgtcggcttc gggatcgata cccatttgga ccccatccac
     3721 ccaggcttcg cggtttttca tgctgccgga cagattgaga taaccgtatt cggacgcaga
     3781 accggagcca gccgcgccct ggttggttcc acaggctgcc agcagcagaa tcagcagggt
     3841 ggcgagaagc aggaagcgtg ttttcatgtt ttattccttg ttagtcattg attttggatg
     3901 ctcacgctta acctaaacgg aacaggactc cgctgtcaag caaaaagatg ccgcaagctt
     3961 ttttccggtg tcgccttatg gttccgcgag gcatataaag tattgacaca atcaggggaa
     4021 acgattttct gacccaacca tcaagcaaca ttgtttcgcc ggtctggaca cgggcaggcg
     4081 cacatgatat aaggaactta tttatgctgg aaaaactact ccgcaagatt ttcggggaca
     4141 agagcacccg cgacttgaac ctgtattcgc cccaggttga ggcgatcaac ctcatctttg
     4201 aaggcttgca ccagtatgac gacgatcagc tccgggaacg ggtggcagac atccgcggcg
     4261 aaattgctga aaaactgcgc gacctgcggg aaacgctgga tgacctggaa cgcagattcc
     4321 gtgaagaagc agaggaaagc gaacgcaacc gcatcgacaa ccgtatcgat gagaccagaa
     4381 aacagctcaa agcactcacc aaatctaccc tggacgacta tctgccggag gttttcgctc
     4441 tggtgaagga aacctgccgc cgcctcgtgg accaggaatt tgaagtgcgc ggacacatgc
     4501 tgaagtggaa catggtgccc ttcgacgtgc agttgatcgg cggcatggcc ctgcacgacg
     4561 gcaagatcgc cgaaatggcc accggcgagg gtaagactct ggtggccacg ctgccgctgt
     4621 ttctgaacgc cctggtgggc cgcggggtcc atctggtgac ggtgaacgac tatctggcct
     4681 cacgtgacgc ggaatggatg tcgcccgttt tcaacttcca cgggatgaaa gttggctgca
     4741 tcaccaccgg catgagtttt gaggaacgca aggaagccta tctctgcgac gtcacttacg
     4801 gcatgaacag cgaattcggt tttgactacc tgcgtgacaa catggcggtt tcgccgcagc
     4861 aactggtgca gcgggatttt tactacgcca tcgtggacga ggtggacagc atcctcatag
     4921 acgaagcgcg cacacccctg atcatcagcg gccccattgc ccaggacaag aatttctacc
     4981 ctgaactgcg gcctcagatc aaccaactgg tgcagtcaca gaacagcatg gtgcagaggg
     5041 tgttaagcga aatccgtgaa gatatccgcg aggattatcc cgcagcggac atggaccgcc
     5101 tggccaccaa cctgctcatc gtgcagcgcg ccgccccgcg caacaaagcc ttcgccaagc
     5161 tgatgcagga aagctggctg aagaagctgg ttaatgacat ggaaggcatc tatctgcgtg
     5221 acaagagaat gcacgagctg gatgagaagc ttttctatgt cgtggaagag cgacagaaca
     5281 gcgttgacct ctgcgaaaag gggcgcgaac tgctttcccg ccatgaaaag aacctctttg
     5341 tggtggaaag cctggatgat gtgctgaacc gggttgacga ggacgaaagc ctctccgaag
     5401 cggacaggat caaacgcaag accctggaaa caaaccgctt catggacaag agcgaaaagc
     5461 tgcacaacat caaccagttg ctcaaagcct acaccctctt cgaaaatgac aaggaatacg
     5521 tggttatcga caacaaggtg atcatcgtgg atgaattcac cggacgccag atgccgggac
     5581 gcaggttctc cgacgggctg catcaggctt tggaggccaa ggaaaacgtc accatcgagg
     5641 ccggaaccca aacttttgcc accatcaccc tgcagaacta cttcaggatg ttcgagcggc
     5701 tttccggcat gaccggcacc gcggtgactg aagaggccga attcatagag atttacaacc
     5761 ttccggtgat gaccattccc accaacgttc ccgtgacccg cgtggaccac gaggacgtga
     5821 tctacatgac caagaacgaa aaatatcagg cactgatgga cgagatcatc tactggcacg
     5881 aactgcaaaa acctgtgttg gtgggcaccg tgagcgtgga ggtttcggaa acgatcagcc
     5941 ggctgctgcg ccgccgcaac attccccaca gtgttttgaa cgcccgccag caccagcgcg
     6001 aggctgagat catcacctcc gcgggaaaac cgggctcggt gaccatcgcc acgaacatgg
     6061 ccggccgcgg cacagacatc aaactgggcc cgggagtggt gacccaaagc accgaaaact
     6121 accgcgggat cgactcagca gtaactgacg ggtttcccta cggccagcct ttggatggct
     6181 tgcacgtgat cggcagcgaa cgccatgaaa gccgccgcat cgaccgccag ttgcgaggca
     6241 gagcagggcg tcagggcgat cccggcacct cgaggttcta cctttccctg gaagatgacc
     6301 tgctacggat tttcggcgca gacaggattt cgagcatcat ggacaggatc ggcatggaag
     6361 agggacagcc tattgagcat cgcctgattt ccaaagccat tgaaaacgcc cagaagcgcg
     6421 tagagggcca gaatttcgac atacgaaagc atcttttgga atacgatgac gtcatgaacc
     6481 gccagcgtca ggttatctat gaa
//
LOCUS       DAOOWR010000057         6455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12085_length_6455_cov_4.104531, whole genome shotgun sequence.
ACCESSION   DAOOWR010000057 DAOOWR010000000
VERSION     DAOOWR010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_6455_cov_4.104531"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLB85_02405"
     CDS             complement(<1..263)
                     /locus_tag="PLB85_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU72207.1"
                     /translation="MKGIYLGLLLLLPLAGLGADTLSPGEFARQIHAEINAIRGRNGL
                     PELIWRDDLAGLALRHSQNMGKQDFFNHVDPEGLQVSQRLRKHL"
     gene            complement(271..720)
                     /locus_tag="PLB85_02410"
     CDS             complement(271..720)
                     /locus_tag="PLB85_02410"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOU72208.1"
                     /translation="MKKLLALCLILAGLSSAFAQAKRVPTRAVLYSAAFPGGGQLYNR
                     AWLKAGLVMGVQGFFIGSAIYHGGERNYWRDLAENTPDPFLQEQYLARSRDFGNELKN
                     DIWWLGITAGLSMLDAYVDAHLSDFESEKEKIRLRFSGDALLLQYNF"
     gene            950..3058
                     /gene="fusA"
                     /locus_tag="PLB85_02415"
     CDS             950..3058
                     /gene="fusA"
                     /locus_tag="PLB85_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU72209.1"
                     /translation="MNSDSDSPLRRIRNIGIMAHIDAGKTTTTERILFYTGFLHRMGE
                     VHDGNAFTDWMEQERERGITITSATVTCVWKDNHINIIDTPGHVDFTAEVERSLRILD
                     GAVGVFCAVAGVEPQSETVWHQADRYKVPRIAYVNKMDRPGADYDRVIGMIHERLTPL
                     ARAVNIPIGREDSFEGVIDLITMKAWLFDPLTKGNEVHLKDIPPELLEQATKQRDILL
                     ESVAEFDDELLHAYLEGLEIPENLLRRAIRVATIGGNIIPVFCGSSLKNKGVQLLLDG
                     ICDFLPSPLEVPPCEGFNPETKAPVTLESDPRAPFVGMAFKVQTDKYVGKLVYVRVYS
                     GTLKKGASFYNQSNGKRERVSRILQMMSNRKNDLNELHAGDIGAFVGCRFLSTGDTIT
                     DGELDVQLSRMHFPDSVISIAIEPRTKADQENLSDALARLEEEDPTFRVTTDKETGQT
                     LISGMGELHLEIIVDRLRREFNVSANVGNPQVSYKETITRAVECEETFIRDMNGKGNY
                     AKVKFRLSPLSEEELPEGAKNLFQSSVGTDKIPGEYHKPIEEAALNALNDGPLISGNL
                     ERVRVELIDGDFNPVDSNELAFRIATSMAISKGLREAVPVIMEPLMQLSVISPDAFVG
                     DIIADINAKRGQISVLRRHNEFQQEIVAEVPLSELVGYTTRIRSLSQGRATYSLEFRK
                     YEISPAQVQSAVLKRIRGFA"
     gene            complement(3394..3858)
                     /locus_tag="PLB85_02420"
     CDS             complement(3394..3858)
                     /locus_tag="PLB85_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72210.1"
                     /translation="MNRLMALVGMIALLAGCCVAKTDRAGVKALLWSDGEALPCEQAD
                     SLAQSALELLQSADEEARLIVSPDLIDSIRSSGTALEIIWPETLAFNLPSGAKDILGR
                     VIIPLKGEYASDGAGALIFFGTKSYGTPALLNHAAAEKLARIEALLENENMN"
     gene            complement(3866..6148)
                     /locus_tag="PLB85_02425"
     CDS             complement(3866..6148)
                     /locus_tag="PLB85_02425"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72211.1"
                     /translation="MNKLTRLTPILLIALLLLLGACAWLQPKDDENPVIQISTPTNYF
                     SYATTQETIDLAGTAFDNRDIKSVTVKVNNSSPVTATGTTNWSLKDIALAKGDNIIVC
                     EAKDKKGNSGTATIIVTRNTDIDFTGHPTFSQSSFFVSQAGYSVVRQTLTPVNCGVSE
                     VQVARVDSNFEVINEIGQIYDDGDLYDHHDEILGDGTYSNKLSILENYAGQYYYRIVA
                     YSDAKVANYSPLYKITVHEAITQDDINKMLDNHAGIAAALNASDATNLEQGAEELKTW
                     FEGRPGVSKAELVDGSLEVTYDTGIKGGVIFCETDENGYITTKGGSATPTRPNANIPL
                     RKQTRGQNALENAIANLNWNTAKVDDADKIQDKDVLIWAPYEGVFDIDMRPSLQTIFE
                     NSDLGLNVVSLRNQQCTVASLANLAEYGTVILDTHGASGEHILTGELMNVVNIWSHIF
                     QILNNEVGFFERVTYSTVGGFAQKGTVYSVRSAYIANLGGTMPNSLIFNGSCESSQTA
                     NLSNAFLGKGAKAYFGFSKIVSTSFCKAKADEIFQKMAVELKNNGKAFVAGQQDPFFS
                     HATFTMNATTNELHYSFDLINGNFEFGNLSGWFREGDGRVITQLGDQQPTEGNFMGII
                     STGLGYTESSGSISQPFRVPDSVETLSIRWNFISEEFMEYVGSQYQDYLTISITDSTG
                     AVHVIFHETIDSFVDYGLVCCTPPISFDQGDCYMTGWRQLVHDISAFQGQVVRLRIAC
                     GDVGDSIYDSACLLDEITIY"
BASE COUNT         1357 a         1752 c         1898 g         1448 t
ORIGIN      
        1 aggtgtttcc tcagccgctg cgagacctgc aggccttcgg ggtccacatg gttgaagaaa
       61 tcctgtttcc ccatgttttg gctgtggcgc agggctaaac cggccagatc gtccctccag
      121 atcagttccg gaaggccgtt cctgccccgg atggcgttga tttcggcatg gatctgcctc
      181 gcgaactcgc ccgggcttag ggtatcagct cccaggccgg ccagcggcag cagcaacagc
      241 aaaccaaggt aaatcccctt cataagcagc tcagaaattg tattgcaaaa gcaaagcgtc
      301 cccggagaag cgcagccgta ttttttcctt ctcgctttcg aaatcagaaa gatgcgcgtc
      361 cacataggca tccagcatgg aaagccctgc cgtgatgccg agccaccaga tatcgttctt
      421 cagttcgttg ccgaaatcgc ggctgcgggc caggtattgc tcctgcagaa aggggtccgg
      481 agtgttttcc gccaggtcac gccagtaatt tcgttcgccg ccatggtaga tggcggaacc
      541 gatgaaaaac ccctgcacgc ccatcaccag gcccgccttc agccaggccc ggttgtaaag
      601 ctgtcccccg cccggaaagg ccgcggaata aagcaccgcg cgggtcggca cccgctttgc
      661 ctgggcgaaa gcggaactca gtccggccag gatgaggcac agcgccagca gttttttcat
      721 tcggattccc atatcaacga tactcttctt tccaaacaag cctgcttgtc aatcattatt
      781 tcaccccggc atccgcagct ccaattcact gtgacgcggg gccggaatga gttgtggtaa
      841 agctgcggcc tcgccggtgt cgggaacttt ttagtggaca aagtttccgc ctcgtatttc
      901 ttggattttt caacaataca cattgaaagg tgagtttagg aagcagatta tgaacagcga
      961 ctccgacagt ccgctacgca ggatcaggaa catcggcatc atggcccaca ttgatgccgg
     1021 caaaaccacg accacggagc ggatcctgtt ttacacggga tttttgcaca ggatgggcga
     1081 ggtgcatgac ggcaacgctt tcaccgactg gatggaacag gagcgtgaac gcgggatcac
     1141 cattacctcc gccacggtga cctgtgtctg gaaagacaac cacatcaaca tcatagacac
     1201 ccccggacac gtggatttca ccgccgaggt ggagcgcagc ctgcgcatcc tggatggcgc
     1261 ggtgggcgtc ttttgtgccg tggctggagt ggagccgcaa tcggagactg tttggcatca
     1321 ggccgaccgt tacaaggtcc cgcgcatcgc ctatgtgaac aagatggacc gtcccggcgc
     1381 cgattacgac agggttatcg gcatgatcca cgagcgcctg accccgctgg cccgggctgt
     1441 gaacatcccc atcggccgcg aggacagctt tgaaggagtt atcgacctca tcacgatgaa
     1501 ggcctggctc ttcgaccccc tcaccaaagg caacgaagtg catctgaagg atattcctcc
     1561 agaactgctg gagcaggcca ccaagcagcg cgacatcctg ctggaaagcg tggcggaatt
     1621 tgatgacgaa ctgctgcatg cctacctgga agggctggaa atcccggaaa atctgcttcg
     1681 ccgggccatc cgcgttgcca ccatcggagg caacatcatc cccgttttct gcggctcatc
     1741 gctgaaaaac aaaggcgtgc aactgctgct ggacggcatc tgcgactttc tgccctcccc
     1801 gctggaagtg ccgccttgcg aaggtttcaa ccccgaaacc aaagcccccg tcaccctcga
     1861 gtctgatccc cgcgcgccct ttgtgggcat ggctttcaag gtgcaaacgg acaaatatgt
     1921 gggcaagctg gtctatgtgc gcgtctattc cggcaccctg aaaaaagggg cttcgttcta
     1981 caaccagagc aacggcaaac gcgagcgggt gtcacgcatt ttgcagatga tgagcaaccg
     2041 taagaacgac ctgaacgaac tccacgccgg cgacatcggc gcctttgtcg gctgccgctt
     2101 cctgagcact ggcgacacca tcacggacgg cgagctggat gtgcagctca gccgcatgca
     2161 ttttccggac agcgtgatct ccatcgccat cgaaccccgc accaaggctg atcaggaaaa
     2221 cctgagcgac gccctggccc gcctcgagga agaggatccc accttccgcg tgactacgga
     2281 caaggaaacc ggccagaccc tcatttccgg gatgggcgag ctgcacctgg aaatcatagt
     2341 ggaccgcctg cgccgcgaat tcaacgtttc cgccaacgtc ggcaatccgc aggtatccta
     2401 caaggaaacc atcacccgcg cagtggaatg tgaggaaacc tttatccgcg acatgaacgg
     2461 aaaaggcaac tatgccaagg tgaaattccg cctttcccca ctttcggagg aagaactgcc
     2521 tgaaggggcg aaaaacctct tccagagcag cgttggcacc gataaaatcc ccggcgagta
     2581 tcacaaaccg atcgaagaag ccgcgctcaa cgccctcaac gacgggccct tgatcagcgg
     2641 aaacctcgaa cgggtgaggg tcgaactcat cgacggcgat ttcaaccccg tggattccaa
     2701 cgagttggcc ttccgcatcg ccacctcgat ggccatctcc aaaggcctgc gtgaagccgt
     2761 cccggtaata atggaacccc ttatgcagct ttcggttatc tccccggatg ccttcgtggg
     2821 cgacatcatc gccgacatca acgccaagcg cggccaaatc agcgtgctgc gcaggcacaa
     2881 cgagtttcag caggaaatcg tggcggaagt tcccctttcc gaactggttg gctataccac
     2941 caggattcgc tccctcagcc agggccgggc gacttacagc ctggaattcc gtaaatacga
     3001 aatcagcccc gcccaggtgc agagcgccgt gctgaagcgc atccgcggct ttgcctgata
     3061 atcctggcag ccaaacaaaa taagattcag gagtatcggc attctatcta taaggctcac
     3121 aaagcgttgg cttcatccct ttagcctccc cgtctatccg tcctttgccc tttccgaagt
     3181 gcctcttttt cgcctcccgc caaattcccg cctttcatgc gaaaggtgta ggagaggcga
     3241 agtggatgtg aattatcagg ggctaaggac ggacaggcaa ggaattgaac agggaaaaag
     3301 agcgggcgga ccttttctga tccgcccgta atggtttatc caccaggaaa gcgccttgaa
     3361 ccggggtttt ttggcggcgt ttccggagga gggtcagttc atgttttcat tttccagcag
     3421 cgcttctatc cgggcaagtt tttccgctgc cgcgtggttg agcagggcgg gggttccgta
     3481 gcttttggtc ccgaagaaga tgagggcgcc ggccccgtcg gatgcgtatt cgcctttgag
     3541 ggggataatt acgcgcccga ggatgtcttt ggcgccggag ggcaggttga acgcaagggt
     3601 ttctggccag atgatctcca gggccgttcc tgaggacctg atggaatcga tgaggtcggg
     3661 gctcacgatc aggcgggcct cttcatcggc gctttggagc agttccaggg cgctctgggc
     3721 cagggaatcc gcctgttcgc agggcagggc ttcgccatcg ctccagagca gggctttcac
     3781 gcccgccctg tcagttttgg ccacgcagca gccggcgagc agggcaatca ttccgaccaa
     3841 agccataagg cgattcacac tttcctcaat agatggtgat ctcgtccaga aggcaggcgg
     3901 agtcgtagat gctgtcgccc acatcaccac aggctatgcg caggcgcacc acctggccct
     3961 ggaaggcgct gatgtcgtgc accaactggc gccagccggt catgtagcag tccccttggt
     4021 caaaggaaat gggcggagtg cagcagacga ggccgtaatc gacaaaagag tcgatggttt
     4081 cgtggaagat gacgtgcacg gccccggtgc tgtcggtgat ggaaatggtg agataatcct
     4141 gatattggct gcccacgtat tccatgaatt cctcggagat gaagttccat ctgatgctga
     4201 gggtttccac cgaatcgggc acccggaagg gctgggagat ggaaccgctg ctttcggtgt
     4261 agcccaggcc ggtggagatg atgcccatga agttgccctc ggtcggttgc tggtctccga
     4321 gttgggtgat cacgcgacca tctccctcac ggaaccagcc gctgaggttt ccgaactcga
     4381 aattgccgtt gatgaggtcg aaggaatagt ggagttcgtt tgtggtggcg ttcatggtga
     4441 aggtggcgtg gctgaaaaag gggtcttgtt gtccggcgac gaaggccttg ccgttgtttt
     4501 tgagttcaac ggccattttc tggaagattt cgtcggcttt ggccttgcag aagctggtgc
     4561 tgacgatctt tgagaaaccg aaataggcct ttgccccctt gccgaggaag gcgttggaga
     4621 ggttggcggt ttgggagctt tcgcaagagc cgttgaagat caggctgttc ggcatggttc
     4681 cgcccaggtt agcgatatag gcgctgcgaa cactgtaaac ggtgcccttt tgggcaaagc
     4741 ctcctaccgt ggaataggtg accctctcga agaagccaac ctcgttgttg aggatctgga
     4801 agatgtggct ccagatattg acgacgttca tcagttcacc ggtcaggatg tgttcgccgc
     4861 tggcgccgtg ggtgtctaat atcacggtgc cgtattcggc aaggttggcc agggaggcga
     4921 cggtgcattg ctggttcctg agcgagacca cattgaggcc gaggtcgctg ttttcgaata
     4981 tggtttgcag gctggggcgc atgtcgatat cgaagacacc ctcatagggg gcccagatca
     5041 gcacgtcctt gtcctggatt ttgtcggcgt catccacctt ggccgtgttc cagttcaggt
     5101 tggcgatggc gttttcgagg gcgttttgcc ctctggtctg cttgcgcagg gggatgttgg
     5161 cgttggggcg cgtgggcgtg gcggagccgc ctttggtggt gatatagccg ttttcgtcgg
     5221 tctcgcagaa gatcacgcct cctttgatgc cggtgtcgta ggtgacttcg aggctgccgt
     5281 ccaccagttc cgctttggaa actccgggcc ggccttcaaa ccaggttttc aactcctcgg
     5341 cgccctgctc caaatttgtg gcatcggagg cgttgagggc ggcggcgatt ccagcatggt
     5401 tgtcgagcat cttgttaatg tcgtcctggg tgatcgcctc gtggacggtg attttgtaga
     5461 ggggcgaata gttcgcgacc ttggcgtcgg aataggccac gatcctgtag taatactggc
     5521 cggcgtagtt ctcgaggatg ctgagtttgt tggaataggt gccgtcgccg agaatttcgt
     5581 cgtgatggtc gtaaagatcg ccgtcgtcat agatttggcc gatttcattt attacttcga
     5641 aattggaatc cacccgggcg acttggacct ctgagacccc gcagttgacg ggtgtaaggg
     5701 tttgcctcac cacgctgtag ccggcctggc tcacgaaaaa gctgctctgg ctgaaggtgg
     5761 gatggccggt gaaatcgatg tccgtgttcc gcgtgacaat gatggtggcg gtaccggaat
     5821 ttcccttctt gtccttggct tcgcagacga tgatgttgtc acccttggcc agggcgatgt
     5881 ctttcaagct ccagttggtt gttccagtgg ccgttacggg agaagagttg ttgactttga
     5941 ctgtgacgga tttgatatcc ctgttgtcga aagccgtgcc agccaggtct atcgtttcct
     6001 gggtggtggc gtaggagaaa tagttggtgg gtgttgagat ttgaatgacc gggttttcgt
     6061 catccttggg ttggagccag gcacaggctc ccagcaataa tagcaaagcg atcaacagga
     6121 ttggcgttag ccttgtcagt ttgttcatgt ttcctccttt tcaagacagg cttgtgtgtt
     6181 tttctttgtg aaccgccccc gaaactaaag ggggcaaatt gctattccgt ttagaatatc
     6241 aatcgacaaa ttgctgtcaa gtggaaaaac gctgatttta gcgggagtct gcttttttta
     6301 taatctggcg gggatggcct gggtttggga tataaggctg aaagcctttt cagaaaagaa
     6361 ggtagaggca aagacatgtt tggtttcgga gaggaaaaaa agccacagag gggcggactt
     6421 cattcatccg gccggctttg atccgcacta gagca
//
LOCUS       DAOOWR010000058         6431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12159_length_6431_cov_4.222710, whole genome shotgun sequence.
ACCESSION   DAOOWR010000058 DAOOWR010000000
VERSION     DAOOWR010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12159_length_6431_cov_4.222710"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLB85_02430"
     CDS             <1..1088
                     /locus_tag="PLB85_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72212.1"
                     /translation="LNYTDSSVTNGQIYQYHVTAVDLEGFRSLPSNVMEAAPIHFPFD
                     RGFLLVDETRHGNGANISPTDQMVDDFYACVLGDFAHSVWDLESQDPLSLDQVSHYPF
                     ILWHCDDFTDMQIIVYQNLLGSYLLSGGTLLISGWKYPSQFDSAFLNRFLPGITPLLN
                     NSAVLVSAQSDTYPDLYPDPAKLAAPWNGMLPMSYVFAGAANPLYRAEILNGGVNNNE
                     PLAIRVHAGGTMVLLGFPLYFMLQDGVRDFLAQIIPELYPDVAVEDGLCPNAGLALVC
                     APNPFSDELRISCQTGKTGEKVELAIHNVRGQRVGGFQGNSGAKGICQFVWDGRDQHG
                     DPVAPGVYLLEMRTAGERKLLKTLRLR"
     gene            1251..2795
                     /locus_tag="PLB85_02435"
     CDS             1251..2795
                     /locus_tag="PLB85_02435"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU72213.1"
                     /translation="MTTQIIKLREKRDAATLGGGEKRVQEQHEKGKLTARERIELLVD
                     PDSFEEFDLFVTHRCTNFDMDEQVYPGDGVVTGSATINGRLVYLFAQDFTVFGGSLSK
                     TYAEKICKIMDMALKNGCPVIGLNDSGGARIQEGIDALAGYAEIFWRNVMASGVVPQI
                     SAILGPCAGGAVYSPAITDFVMMEKRNSYMFVTGPKVVKTVLNEVVTTEDLGGAEVHS
                     SKSGVAHFITNSEEETLLLIKKLLSYLPSNNMEDPPYVPSHDPIDRACASLDEIIPEN
                     PNKPYDILDVIHEMVDDREFLQVMLNFAQNVVVGFARMNGFPVGIVANQPKILAGVLD
                     IDASIKAARFVRFCDSFNIPLIVLEDVPGFLPGTSQEHNGIIRNGAKLLYAFAEATVP
                     KITVILRKAYGGAYCVMNSRHMRADLVYAWPSAEIAVMGPKGAVEVIFRKEAQASDDP
                     KQTLLEREKEYAEKFANPFVAAERGYIDDIIEPSRTRFRLIRALEMLANKKDSIPPRK
                     HGNMPL"
     gene            2805..3671
                     /locus_tag="PLB85_02440"
     CDS             2805..3671
                     /locus_tag="PLB85_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOU72214.1"
                     /translation="MSKRTIFGLLLLALCLALAAQEVEQPSFEDLQLQIQAMSDSLLK
                     LRESEVVSAYGFKETDKLTDVAAKLEISDLEHWKEYLGIEPKNEVLDKMSLRKLGITP
                     FRAFLAQQYSIYGFTELSSIGELAAQNQLPVKKVRQFAGIDSEDRSFDDYSLQALNRT
                     PEEFVAFEKEFNAKKLNFGAIILGFGILTVFVALAVTALVVGQLKRLNPKPKKADSTL
                     VVTSSGKVKAKPVDMNSHVIAAAIATLHFHKHDIEERRRLLLTFRRAGSDQWRSSMLL
                     NMPNREILRKRS"
     gene            3677..4390
                     /locus_tag="PLB85_02445"
     CDS             3677..4390
                     /locus_tag="PLB85_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU72215.1"
                     /translation="MKTYKLIIGGQRYEARVLEYTTSHAKININGTDYLIQIEDDTLS
                     QVPVLPQQEHSVPLAPAFSSDFAAGSGEVRAPLPGVIASIRVKEGEAVKKGQTILVLE
                     AMKMESEIAAPVDCVVEKIHVKDRAPVQEGDLLMTLSGFEIKEQPAPKTTRQQTPAPA
                     AKSAPADRIVRAPLPGLIIDLKVQPGDFVQEGTTLLILEAMKMESDIHSNLSGKVLKI
                     HVQKGDTVQEGDPLVELEA"
     gene            4393..5520
                     /locus_tag="PLB85_02450"
     CDS             4393..5520
                     /locus_tag="PLB85_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU72216.1"
                     /translation="MQEFLQVLNTTGFVNFSWGNVVMILAGIVFIYLAIVKGFEPLLL
                     VPIGFGIVVGNIPGLLEQGLGVVSEGSVLNYLYFGVNKGIYPSLIFLGIGAMTDFSTL
                     IANPKLILLGAAAQVGIFGTFLGALLLGFNVLEAASIGIIGGADGPTSIFLASKLAPN
                     LIGPIALAAYSYMALVPVIQPPIMKLLTTPKERLIRMKPPRQVSKKEKIFFPVITFLV
                     TAIIAPGGAILLAMLFLGNLLKECGVTERLALTARTSLIDIVTVLVGFTVGAKTTADV
                     FLTPQSLGIFFLGAFAFAIATACGVLFAKFMNLFIKDKINPLIGNAGVSAVPASARVS
                     QVMGQKYDPTNYLLMHAMAPNVAGVVGSAVAAGVLLGILGS"
     gene            5591..>6431
                     /locus_tag="PLB85_02455"
     CDS             5591..>6431
                     /locus_tag="PLB85_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72217.1"
                     /translation="MKKTCIAILILALSAFLGATKYAGEIFQLSSGVQNQAMGNTGLT
                     FGNSLAAGWWNPALLAEPGSCGVELMRVEHFEGLMQQNQLGVVFGTKTRTSVQINHLG
                     IDEIKLTQLENPADSLSNSNRPEVWKTVGNHDLIVYGSLARSLKNNLHLGLTPKLAYR
                     SLAENSGFGIGADLGLLWDAGKGFRAAANLRDFFSTQLIWANGTHEIAIPNLDLEFGY
                     GFSPFKEIPVHLALRTQIFAEGRGEASNVAAGPVSADFHAGLLVKPIPALNVMAGWDS
                     DCFT"
BASE COUNT         1433 a         1864 c         1772 g         1362 t
ORIGIN      
        1 cattgaacta cacggactcc agcgtaacca acggccaaat ctatcaatac catgttaccg
       61 ctgtggacct ggaaggcttt cgcagcctgc cttccaacgt gatggaagcg gcgccgatcc
      121 actttccctt cgaccggggt tttttgctgg tggacgagac caggcatggc aacggggcaa
      181 atatctcacc caccgaccag atggtggatg atttctacgc ctgcgttttg ggtgattttg
      241 cccactcggt ttgggacttg gagagccagg acccgctcag cctggaccag gtgagccact
      301 atcccttcat cctctggcat tgcgacgatt ttacggacat gcagatcatt gtctaccaaa
      361 accttctggg cagttacctg ctaagcggcg gaaccctgct gatatcgggc tggaaatacc
      421 ccagccagtt cgacagcgct ttcctgaaca ggtttctgcc gggaatcacg ccgcttttga
      481 acaattccgc cgtgctggtt tccgcgcaga gtgacacata tccagacctc tatcccgacc
      541 cggccaagct ggcggcgccc tggaacggga tgctgccgat gagctatgtc tttgcgggag
      601 cggcaaatcc tctctaccgg gcggagattt tgaacggtgg ggtgaacaac aacgagccgc
      661 tggcgatcag ggtccatgcc ggcggaacca tggtgctgct tggctttccg ctctacttca
      721 tgcttcagga tggggtaagg gactttctgg ctcagataat cccggaactc tatcccgatg
      781 tggcagttga ggatggcttg tgtccgaatg ccggcctggc gcttgtctgc gcgccgaatc
      841 cattttcaga tgaactgcgg ataagctgcc aaaccggcaa aaccggtgag aaagtggaac
      901 tcgccatcca taacgttcgg ggacaaaggg ttggagggtt tcagggtaat tccggcgcca
      961 aaggaatttg ccagtttgtt tgggacggac gcgaccagca cggcgatcct gtcgcccccg
     1021 gggtatatct gctggaaatg cggactgctg gtgagcgcaa gctcttaaaa accttacgct
     1081 tgcgctgatt ctggaaaggc ctgaccgtcc gtcatttctc caacggaata ataaaccttt
     1141 acgagaaaac ggagttaaat taaatggccc ccaaatgacc ggctccggga ccgtaactcc
     1201 gtgcccggac aggattggaa tcaatcatca gccaaaacaa ggattgaaat atgacaaccc
     1261 agatcatcaa actgagggaa aagcgcgacg cggcgaccct gggcggcggc gaaaaacgcg
     1321 tgcaggagca gcacgaaaaa ggcaaactca ccgccaggga gcggatcgag ctactggtgg
     1381 accctgacag ttttgaggaa tttgacctct ttgtaaccca ccgctgcacc aactttgata
     1441 tggacgaaca ggtctatccc ggtgacggcg tggttaccgg tagcgccacc atcaacggac
     1501 gcctggtcta tctgtttgcc caggatttta ctgtcttcgg cggctcactt tccaagacct
     1561 acgcggaaaa gatctgcaag ataatggaca tggcacttaa gaacggctgc ccggttatcg
     1621 ggcttaacga tagcggcgga gcgcgcatcc aggaagggat cgacgccctg gccggctatg
     1681 ccgagatctt ttggcgcaac gtgatggcca gcggggttgt tccccagatc tcagccatcc
     1741 ttggcccatg tgccggtggg gcggtctatt cccctgccat cacagatttt gtgatgatgg
     1801 aaaaacgcaa cagctatatg ttcgtgaccg ggcccaaggt ggtgaaaacc gttctgaacg
     1861 aggttgttac cacggaagat ctgggcggcg cggaagtgca ttccagcaag agcggcgtgg
     1921 cgcatttcat caccaacagt gaggaagaga ccctgctgct gataaagaaa ctgctcagct
     1981 atttgccctc caacaacatg gaagacccgc cctacgtgcc atcccacgat ccgatcgacc
     2041 gtgcctgcgc ttcgctggat gagataatcc cggagaatcc caacaagcct tacgacattc
     2101 tggacgtgat ccatgagatg gtggacgacc gtgaattcct gcaggtgatg ctgaatttcg
     2161 cccaaaacgt ggtggtgggc ttcgcgcgga tgaacggttt ccctgtgggc atagtggcca
     2221 accagccaaa aatcctggcc ggggttttgg atatcgacgc ttccatcaaa gccgctcgtt
     2281 tcgtacgttt ctgcgattcc ttcaatatcc ccttgatagt gctggaagac gtgcctggct
     2341 tcctgcccgg caccagccag gaacacaacg gcatcatccg caacggcgcc aagctgctct
     2401 acgccttcgc tgaagccacc gtgcccaaaa tcaccgtgat cctgcgcaag gcctatggcg
     2461 gcgcctactg cgtgatgaac agccgccaca tgcgcgctga ccttgtttac gcctggccct
     2521 cagccgaaat tgccgtgatg ggccccaaag gcgctgtgga agtgatcttc cgtaaagagg
     2581 cgcaggcttc cgacgatccc aaacagaccc tgttggaacg ggaaaaagag tacgcggaaa
     2641 agttcgccaa tcccttcgtc gcggccgaac gaggctacat cgacgatatc attgagcctt
     2701 cgcgcacccg cttccgcctc atccgggctc tggaaatgct ggccaacaaa aaggacagca
     2761 tcccgccccg caagcatggc aacatgccac tttaagatat gatcatgagc aagagaacaa
     2821 tatttggcct tctgctgctg gcgctctgcc tggcgctggc ggcccaggaa gtggagcagc
     2881 ccagcttcga agacctgcag cttcagatcc aggcgatgtc cgacagcctg ctgaaattga
     2941 gggaaagcga ggttgtcagc gcctacggtt tcaaggaaac ggacaaactg acggacgtgg
     3001 ccgccaaact ggagatcagt gacctcgagc actggaaaga gtatctgggc atcgagccca
     3061 agaacgaggt gctcgacaag atgagcctgc gcaagctggg catcacgcct ttccgggcct
     3121 ttctggcaca gcaatacagc atctacggct tcacggagct tagtagcatc ggcgagctgg
     3181 ccgcgcagaa ccaactgccg gtgaagaaag tacgccagtt cgccggcatc gatagcgagg
     3241 acaggagttt tgacgactat tccctgcagg cgctgaaccg aactccggaa gagtttgtgg
     3301 ccttcgaaaa ggaattcaac gctaaaaagc tgaatttcgg agccattatc ctcggttttg
     3361 ggatattgac cgtgtttgtt gccctggctg tcaccgccct ggtggtaggc cagttgaaac
     3421 gtttgaatcc caagcccaaa aaagctgaca gcaccctggt ggtaacgtct tccggcaagg
     3481 tgaaggccaa gccggtggac atgaactcac atgtgatcgc agccgccatc gccacgctgc
     3541 acttccacaa acacgatatc gaggagcggc gcaggctgct gctcaccttc cgccgtgcgg
     3601 gttccgacca gtggcggagc agcatgttgc tgaacatgcc caaccgggaa attctgcgca
     3661 aaaggagctg aaaaccatga aaacctacaa actgatcatc ggcgggcagc gctatgaagc
     3721 ccgggtattg gaatacacca ccagccatgc caagatcaat atcaacggca ccgattacct
     3781 gatccagatc gaggatgaca ccctgtccca ggtgcccgtg ctgccacagc aggaacattc
     3841 cgttccgctg gcgccggcat tttccagcga tttcgccgcc ggctccggtg aggtccgcgc
     3901 tcccttgcca ggggtgatag cctccatccg tgtgaaggaa ggtgaagccg ttaaaaaggg
     3961 ccagaccatc ctggtgctgg aagcgatgaa gatggaatcc gaaatcgccg ctccggtgga
     4021 ttgcgtggtg gaaaagatcc acgtgaaaga ccgcgccccc gtgcaggaag gcgatctgct
     4081 gatgacattg agcggcttcg agatcaagga acagcccgca cctaaaacaa cccgccagca
     4141 aactccggct ccggctgcca aatccgctcc cgcggacagg atagttcgtg ccccgctgcc
     4201 cggcctcatc atcgatctta aggtgcaacc cggcgatttt gtgcaggaag gcaccactct
     4261 gctgatcctg gaagcgatga agatggaatc cgatatccac agcaacctga gcggaaaagt
     4321 gctgaaaatc cacgtgcaga aaggcgacac cgtgcaggaa ggcgatccgc tggtggagct
     4381 ggaggcctaa acatgcagga atttttgcag gttctcaaca ccaccggctt cgttaatttc
     4441 agctggggca acgtcgtgat gatcctggcc gggatcgtgt tcatctacct ggccatcgtg
     4501 aaaggctttg agccgctgct gctggtgccg atcggttttg gcattgttgt gggcaacatt
     4561 cccggcttgc tagagcaggg cttgggtgtg gtaagcgaag gcagcgtgct caactacctc
     4621 tattttgggg tgaacaaagg catctaccct tccctgatct tcctcggcat tggcgctatg
     4681 accgattttt ccaccctgat cgccaatccc aagctgatcc tgttgggtgc cgcagcccag
     4741 gtgggtattt tcggaacttt cctgggcgct ttgctgctgg gctttaatgt gctggaagca
     4801 gcctccatcg gcatcattgg cggtgcggac ggccccacct ccatcttcct ggcctcgaag
     4861 ctggctccca acctcattgg ccccatcgcc ctggccgcct attcctatat ggctttggtg
     4921 ccggtgatcc agccaccgat catgaagctg ctcaccacac ccaaagagcg cctgatccgc
     4981 atgaaaccgc cccgccaggt ttccaagaaa gaaaagatat ttttccccgt tatcaccttt
     5041 ttggtgacag ctatcattgc cccgggaggg gccatcctgc ttgccatgct ctttttaggc
     5101 aatctgctca aggaatgcgg cgtcacggaa cgtcttgccc tcacggcccg cacatccctg
     5161 atcgacatcg tgaccgtgct ggtgggattc accgtgggcg ccaaaaccac cgccgacgta
     5221 tttctgacgc ctcagtctct gggaatcttt ttcctcggcg cttttgcctt cgcgatcgcc
     5281 actgcctgcg gtgttctatt cgccaaattc atgaatctgt tcatcaaaga caagatcaat
     5341 cctctcatcg gcaacgctgg cgtttccgcc gtgcccgcca gtgcgcgcgt ttctcaggtg
     5401 atgggtcaga aatacgatcc caccaactat ctgctgatgc atgccatggc cccgaacgtg
     5461 gccggggtgg tcggttccgc cgttgccgct ggtgtgttgc tgggaatttt gggaagctga
     5521 agcctaccgc ggaaaatatt tctttaccga aaacgctgta cggattatca tgtccgcata
     5581 ggtaagaatc atgaaaaaaa catgtatagc catcctcatt ctggcgcttt cggcatttct
     5641 gggagccacc aaatacgccg gtgagatttt tcagctctct tccggcgtgc aaaaccaggc
     5701 gatgggcaac accggcctca catttggcaa ctccctggcc gcgggttggt ggaatcccgc
     5761 tctgctggcc gaacccggct cctgcggagt cgagctgatg cgggtggagc attttgaagg
     5821 cctgatgcag cagaaccagc tgggcgtggt gttcggcacc aaaacccgca cctccgttca
     5881 gatcaaccac ttgggcattg acgaaatcaa actcacccag cttgaaaacc ctgctgacag
     5941 cctgagcaat tccaaccgcc ccgaagtttg gaaaaccgtg ggcaaccatg acttgatagt
     6001 ctatggcagc ctggcccgct ctctgaagaa caatctgcat ctgggcctca cgcccaaact
     6061 ggcttaccgc agcctggcgg aaaactccgg tttcggcatc ggggcagacc ttggcctgct
     6121 ctgggacgcc ggcaaagggt tccgggccgc ggccaacctc cgtgatttct tttccaccca
     6181 actcatctgg gccaacggca cccacgaaat cgccataccc aatctggacc tggaattcgg
     6241 ctacggtttt tctcccttca aggaaatccc cgtccacctg gccttacgca cccagatttt
     6301 cgcggaaggc cgtggcgaag cctccaacgt ggctgccggg cctgtgagcg cggactttca
     6361 tgccgggctt ctggtaaagc ccattccagc gctgaatgtg atggcgggct gggattcgga
     6421 ttgcttcacc g
//
LOCUS       DAOOWR010000059         6304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12475_length_6304_cov_3.752920, whole genome shotgun sequence.
ACCESSION   DAOOWR010000059 DAOOWR010000000
VERSION     DAOOWR010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12475_length_6304_cov_3.752920"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PLB85_02460"
     CDS             complement(<1..1651)
                     /locus_tag="PLB85_02460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU72218.1"
                     /translation="MKRLILLVLALTAAFLSAKDPDPKAVVGKIDNKTYTYSEYNKIL
                     NNYYKYHQNQKGSALTKEEKADLNNRCWEELVGRYIYDKAIKAGKIKITQQELLREAK
                     KNPPAAVKQIPDLKTNGKFDKKKYEKALTEAKEFRNAVLDEVKNLYQYSKLLETIRSE
                     AVVVEDSVRAQWERDTEVVDAKIIFFDANRQPSVVASEDEARDYFEARKEEFRKDDCR
                     RYRFVKFAKAPTVADSLAVHEEALRIYRELQSGADFAELAREFSDDLGSGSKGGDLGW
                     FGRNRMVKVFEDTAFSTPVGEISEPVLSQFGWHIIQTQDRRGTEDNEEVMARHILIKM
                     EPSMATQQAMKAQSAQVFELAKQNGLIAAAAEMNLKVEESSVFQAKDAFIPGIGRDAG
                     LVSFAFDNPEGALADMFTSPSGDIFVCEVSGVYQVWYPTFEDERGRVMSNATSSKRGY
                     TMNARVQEFVRNIKPEHYLSEAERDSILVVEISGHKKGDNISSIGKVQAIEDALFSTP
                     EGTFAPLISEAMRWFLVKVDRHQVPDPAEWEKDKKKLLSEAK"
     gene            1911..2582
                     /locus_tag="PLB85_02465"
     CDS             1911..2582
                     /locus_tag="PLB85_02465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU72219.1"
                     /translation="MNRQTGSKIIATLLLLLLFSALAWGKSAQSAGKSETTATAQTQS
                     DNVTKDGALRVLPAGTYSLQAWENRQPRNITEGYYIIINKTKHKLHLYKDGALLKTYP
                     VATGRNKKDKSSVGDLATPEGHFNVASINASIKSRYICPKTGKQSKPGVYGPWFFVLN
                     TQSGAFSGKSWTGIGIHGTSSPSSIGKSVSHGCIRLYSKDITEIREEVAWLDDTSQIR
                     VDILK"
     gene            2579..3433
                     /locus_tag="PLB85_02470"
     CDS             2579..3433
                     /locus_tag="PLB85_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOU72220.1"
                     /translation="MKKTVSGLIVILLLLAILITGACGDRKDTQGQTRIVALSPEVAE
                     IIASLGGTSLLVGVTQECKYPPELARIKKVGNFGAVSREAVLALKPDLVFTSALEQDA
                     LTTDLAKMGLTVKQIYPKKIDDLPLVVLQIGELIGKTEEAKILADSLSSGIAAMRDQT
                     AGKTRPKVYLEIYRDPLMSVSDDSFVGEIIEAAGGDNIFSTLERDYARVSPEAVVKAQ
                     PDIIICYSRDSLESILARKGWQDIPAVRSRRVYFETDIDPDLIQQATPRTLEGMRRLY
                     KLFFPSEE"
     gene            3434..4354
                     /locus_tag="PLB85_02475"
     CDS             3434..4354
                     /locus_tag="PLB85_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU72221.1"
                     /translation="MKRNRIFTLLLVLAGGLVVAFYLFGGGVDANIVTELRLPRLILT
                     VFTGMTLAGIGSVYQLMLANPLAEPYILGISSGSAFGSILFGVLGLTLLMPLGGFIGA
                     VATLLLVWRLAQKKGSFDRSRLLIAGVIAGMFFAAGISLVMYLFQKDTMIILGTLMGN
                     LGHVFTRGEWSFFLVLMGVSLLILAWLWFRAPALDIMSGGDIYAGSVGINVSRVRKEI
                     FVFTSLLIGITVSYAGIIGFVGLIIPHVVRFFVPSGQKRVYLWSLAAGGIFLLLGDLV
                     AKNIAAIELPVGVVTAAVGCPFFVWLMLRK"
     gene            4870..5559
                     /locus_tag="PLB85_02480"
     CDS             4870..5559
                     /locus_tag="PLB85_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU72222.1"
                     /translation="MPKPRIGLSMNYMRLGSHHQFHVRDKYIDAVFAAGALPCPLPCT
                     DDKAALRQYLEPLGAVIVIGGLDYPPELYGEALHPKTELAHERRVKGDFALLETALEL
                     EKPLLGICAGMQLLNVFFGGALIQHIPELDAHYGEKQHPVEILGGRWLPRIYGEGRIT
                     VNSNHHQAADPNRIGAGLKVVARAEDGMVEALEHDSEQMVLGIQWHPERMADSALGGA
                     VFRFLVKLAKT"
     gene            complement(5605..>6304)
                     /locus_tag="PLB85_02485"
     CDS             complement(5605..>6304)
                     /locus_tag="PLB85_02485"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU72223.1"
                     /translation="RLSKIEIVPPTKRLPVIFAPLSLYALLSRFEAAWSPVNIHNGVS
                     PLCGKLGERIVSEKISLTREPFDLELDSASAFDAEGTPTRRLTLIDKGVFSAFPTDLN
                     YAAKLNLEPNGCAVRQSLEWLPTANAVNLVLDPGDISLEDMVSGIKEGILAQFLMGAH
                     SGNVLNGDYSVGVSTGFMIENGRITGRVKDCMLSGNVYETLSNVEAVENKALNLGIHK
                     LPALLCKDVSVAGK"
BASE COUNT         1356 a         1741 c         1739 g         1468 t
ORIGIN      
        1 ctttggcttc tgaaagcagc ttctttttgt ccttttccca ttcggccgga tctggtacct
       61 gatggcggtc aaccttcacc aaaaaccagc gcatcgcctc actgatcaag ggcgcgaaag
      121 tgccttccgg agtgctgaac agggcatctt caatggcctg gactttgccg atggagctga
      181 tgttgtcgcc ttttttgtgg ccgctgatct ccaccaccag gatgctgtcg cgttccgctt
      241 cgctcaggta atgctcaggt tttatgtttc tgacaaactc ctgcactctg gcgttcatgg
      301 tgtatccgcg cttggagctg gtggcattgc tcatcacccg gccgcgctcg tcttcgaaag
      361 tgggatacca gacctgataa acgcctgaaa cctcgcagac aaaaatgtcc ccggagggtg
      421 aagtgaacat gtcggccaaa gcgccttcgg gattgtcgaa ggcgaaactc accaggccag
      481 catccctgcc gatgccgggg atgaaggcgt ccttggcttg gaaaaccgag ctttcctcca
      541 ccttcaggtt catctcagcc gccgcggcta tcaggccatt ttgtttggcc agctcgaaaa
      601 cttgcgcgct ctgagctttc atggcctgct gggtggccat actgggttcc atcttaatca
      661 ggatatgccg ggccataacc tcttcgttgt cttcggtgcc gcgccggtcc tgggtttgga
      721 tgatgtgcca gccaaactgg gaaagcaccg gttcggatat ctcgcccacc ggagtgctga
      781 aggctgtgtc ttcaaacact ttaaccatcc tgttccgtcc gaaccagccg aggtctccac
      841 ctttcgagcc ggaaccgagg tcatcggaaa attccctggc caattcagca aaatcagccc
      901 cagattgaag ttcacggtag attcgcagcg cctcttcgtg aacagccaga gaatctgcca
      961 cggtaggggc tttggcaaac ttcacaaagc ggtagcggcg gcagtcgtcc ttgcggaatt
     1021 cttctttgcg tgcctcaaag taatcgcggg cttcgtcttc gcttgccaca acgctgggtt
     1081 ggcggttggc atcgaaaaag atgatcttcg cgtccaccac ctcggtgtcc cgttcccact
     1141 gggccctaac gctgtcttcc accaccacgg cctcggagcg gatcgtctcc agcagcttgc
     1201 tgtattggta taggtttttc acctcatcca gcacggcatt gcgaaactcc ttggcttccg
     1261 tgagagcctt ttcatatttc tttttgtcga actttccatt ggtcttaagg tccgggatct
     1321 gtttcaccgc ggcaggcgga ttcttcttcg cctcgcgcag aagttcctgc tgggtgatct
     1381 tgattttacc ggctttgatg gctttgtcgt agatatagcg acccaccagt tcttcccagc
     1441 agcggttgtt caggtcggct ttctcttctt tggtgagggc gctacctttc tggttctggt
     1501 ggtacttgta atagttgttc agaatcttgt tgtattccga gtaagtataa gttttgttgt
     1561 cgattttgcc gacaacagcc ttgggatctg ggtccttggc gctcaggaaa gcggccgtga
     1621 gcgcaagcac cagcaaaatc agtcgtttca tctgatctcc tgaaatatct attgccttgt
     1681 atataccatt attagtgtct gttccctgta aatttggctc tccggattac gtcaaccgaa
     1741 aaatccattt ctgccgctgg cctttatgcg cttcgcgagc aaaagataac gcaggaatcc
     1801 ctttggaatg gggtataaaa agcagttgac aaatatcctt gtcggctttg tctgacgcta
     1861 tcagattagg agatcgagcc tgtaactaat tccgagtatg agaggttaaa ttgaatcgac
     1921 agaccggcag caagatcatc gcgaccctgt tgcttttgtt attgttttcg gcattggcgt
     1981 ggggcaagtc agcccagtct gctggaaagt cggaaacaac cgctacggcc cagacgcaga
     2041 gtgacaatgt taccaaagat ggcgccctca gggtgcttcc tgccggtacg tacagcctcc
     2101 aagcctggga aaaccggcag ccaaggaaca tcaccgaagg ctactacatc atcatcaata
     2161 aaaccaaaca caaacttcat ctgtataaag acggcgcgct gctgaagacc tatcccgtag
     2221 ctactggcag gaataagaag gacaaatcca gtgttggcga ccttgccacc cctgaaggcc
     2281 atttcaatgt tgcttccatc aacgcttcca tcaaaagtcg ttatatctgt ccgaaaaccg
     2341 gaaagcaaag caagcctggg gtttacggac cctggttctt tgtcttgaac acccaatccg
     2401 gcgccttctc cggcaaaagc tggaccggga tcggcattca cggcaccagc agcccctcct
     2461 ccatcggcaa atccgtatcc cacggctgca tccgcctata tagcaaagac ataaccgaga
     2521 tcagagaaga ggtcgcctgg ctggacgata cttctcagat ccgggtggac atcctgaaat
     2581 gaaaaaaact gtttctggct taatcgtaat cctgttgctg ctggcaatcc tgatcacggg
     2641 cgcctgtggc gataggaagg atacgcaagg gcaaacccgg attgtagccc tctctccgga
     2701 agtggcggaa atcatagcct cgcttggagg gacatctctt ttggttggcg taacccagga
     2761 atgcaaatat ccgcccgaac ttgccaggat aaagaaagtg ggcaatttcg gggccgtgag
     2821 cagggaagcc gtcctagccc tgaaaccgga cttggtgttc acctccgctc tggagcagga
     2881 tgccctcacc accgaccttg ccaaaatggg actcaccgtg aagcagatct atcccaaaaa
     2941 gattgacgac ctgcccctcg tggttctgca aatcggagag cttatcggca aaacggagga
     3001 ggcgaaaatt ctggccgaca gcctcagcag cggcatagcg gctatgcggg accagacagc
     3061 cggcaaaacc cgccccaaag tatatctgga gatttaccgc gatcccctga tgagcgtatc
     3121 cgacgactcc ttcgtgggcg aaataataga agccgccggg ggcgacaaca ttttcagcac
     3181 cctcgagcgg gattacgccc gcgttagccc tgaagccgtg gtcaaagcac agccagacat
     3241 cattatctgc tattcccgcg acagcctgga atccatcctg gcccgcaaag gctggcagga
     3301 tattcccgcc gtccgtagcc ggagggtgta tttcgaaacc gatatcgatc ccgacctcat
     3361 ccaacaagcc accccgcgca ccctggaagg gatgcgacgc ttatacaagc tgtttttccc
     3421 atcagaagag tagatgaagc gaaaccggat ctttacgcta ctgctggtgc tagccggcgg
     3481 attggtggtg gccttctatc tctttggcgg cggagtggat gccaatatcg taacggaact
     3541 gcgcctgccg cggctgatcc tgacagtctt taccggcatg accctggccg ggatcggttc
     3601 cgtatatcaa ctgatgctgg ccaatcctct ggctgagcct tacattctgg gcatctcctc
     3661 cggctcggct ttcggctcca tccttttcgg agtgctgggg ctcaccctac tgatgccttt
     3721 gggcggtttt atcggcgcgg tggcaaccct gctgctggtc tggcggctgg cccagaaaaa
     3781 aggcagtttc gaccgcagcc ggctgctgat cgcgggggtg atcgccggaa tgtttttcgc
     3841 ggcggggatt tcactggtga tgtatctttt ccaaaaagac accatgatca tcctgggcac
     3901 cctgatgggc aatctgggtc acgttttcac ccggggcgaa tggtcttttt tcctcgtgct
     3961 gatgggtgtt tcgctgctga tcctggcctg gctctggttt cgtgctccgg ctctggatat
     4021 aatgagcgga ggcgatattt acgccggcag tgtgggcatc aacgtttccc gcgtgcgcaa
     4081 ggagattttt gtcttcacct cgctgctaat cggcatcacg gtttcctatg ccggcatcat
     4141 cggtttcgtg ggcctcatca ttccccacgt agtgcgcttt ttcgtgccat ccgggcaaaa
     4201 aagggtctat ctctggtcac tcgccgctgg gggcatattt ctgctgctgg gagacctggt
     4261 cgccaaaaac atcgccgcca tagaactacc cgtgggcgtg gtcacagcgg cggtcggctg
     4321 cccctttttc gtctggctga tgctgcggaa atagcgcaac ctgacagact ccaattacca
     4381 tccatccagt tcaatcagcc atttggcttt ctttcaaatc tagtagctga aactcagtgt
     4441 cttacaaccc agtaggcgac agagatcaat ctcctcggaa gagtgaagcg atgcctgctg
     4501 gccgttaagg ccttgtcctg tgcatcaggt gcgcctcccg tataataccc gcatttgatg
     4561 cgagtatttt acaagaggcg tgagagaggg acgggctcaa gcgctcagga cctgactgac
     4621 aggaccttat attgggttaa catttctaac tctcttttat accgattggc tgcggttact
     4681 caaaaagaaa gcgggccaga gggcgtttac catccgattt tatagagagc caaatggaaa
     4741 gatagaacaa gtgatggagg agaaatgagg cccaactcct ttttggggca aggacatgcc
     4801 agaatcagct tgacagcttg ggcccgtccg ctggtgtgaa cgtttcaata aacgaacccg
     4861 aggtatccca tgccaaaacc ccgaataggc ctgagcatga actacatgag gctgggcagc
     4921 catcaccagt tccatgtgcg ggacaaatac atagacgcgg tttttgccgc gggcgcgcta
     4981 ccctgtcccc tgccctgcac ggacgacaaa gccgcgctgc gccagtatct ggagcctttg
     5041 ggcgcggtta tcgtgatagg cggtctggat tacccgccgg aactttacgg cgaggcgctc
     5101 caccccaaaa cggagctggc tcacgagcgc cgcgtgaagg gagacttcgc ccttctggaa
     5161 accgctctgg aactggagaa accgctgctg ggcatctgtg ccgggatgca gcttctcaac
     5221 gtctttttcg gcggcgccct gatccagcac atccccgaac tggacgccca ttacggggaa
     5281 aaacagcatc cggtggaaat cctgggcgga cgctggctgc cgcggattta cggcgaaggc
     5341 aggatcacgg tgaattcaaa ccaccaccag gctgccgacc ccaatcgaat cggggcaggc
     5401 ctgaaagtcg tggcgcgggc tgaagacggg atggtggaag cgcttgagca cgattcagaa
     5461 cagatggttc tgggcatcca atggcatccg gaaaggatgg ctgattcagc gctgggcggg
     5521 gcggtgttca ggtttctggt aaaactcgcc aaaacataga aggggcaccg gtttggcgcc
     5581 ccttcaggga atgcagttat agtcttattt gcccgccacg ctcacgtcct tgcaaagcag
     5641 ggcgggaagc ttgtggatac cgaggttcag ggccttgttt tccaccgcct ccacgttgga
     5701 gagggtttcg taaacgtttc cggatagcat gcagtccttc acccggccag tgatgcggcc
     5761 gttttctatc atgaaaccgg tggagacgcc gacagaataa tcgccgttga ggacgttgcc
     5821 ggaatgcgct cccatcagga actgggccag gatgccctcc ttaatgccgg aaaccatgtc
     5881 ctccagggaa atgtcgccgg gatccagcac cagattgacg gcatttgccg tggggagcca
     5941 ttccaatgac tggcggaccg cgcagccgtt gggctccaga ttcagtttgg cggcataatt
     6001 caggtcggtg gggaaggcgc tgaaaacacc tttgtcaatc agcgtcagcc ggcgggtggg
     6061 ggtgccctcg gcgtcgaaag cggaggcgga gtccagctcg aggtcgaaag gctcgcgggt
     6121 gaggctgatc ttctcggaga cgatcctctc ccccagcttg ccgcagaggg gcgaaacccc
     6181 gttgtggatg ttcactggcg accacgcggc ctcgaacctt gagagcagcg cgtagagcga
     6241 aaggggggcg aaaatcacag gcagcctctt ggtgggcggg acaatctcga ttttgctgag
     6301 gcgg
//
LOCUS       DAOOWR010000060         6269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12555_length_6269_cov_4.269070, whole genome shotgun sequence.
ACCESSION   DAOOWR010000060 DAOOWR010000000
VERSION     DAOOWR010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_6269_cov_4.269070"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1519)
                     /locus_tag="PLB85_02490"
     CDS             complement(74..1519)
                     /locus_tag="PLB85_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOU72224.1"
                     /translation="MKTANIKLDYDFAGKDTVEIENKMAPLHIGRSESGQVEIRAELS
                     IHSFVPGTDMAENFHVGEEGGTLKIYLERIPELAESFMGTGRSQLWIRVPQGAAVIAK
                     ADNLPIRAEGIAGSLALHNENGSLKLEDCEGHIHLKNENGSTKLKSCSGDFKIRLENG
                     PLSAEKLSGANLEVKSENGPVKLRLASFARAKVSNENGVIFYESLPLDSGDLEFVNEN
                     GVVKLVLAEEQNFEMTAETSVGSISVTGGSEGDSGAGGLKSRGETENGVRREIISRGE
                     GGLKISVRTENGVIKLNADGNNDMNFVKMKVNDLKGAIGNAVGEEDMEKVRAALNSAT
                     AAVEKAIGSISEEKIKEKLSGALGKLQKTVEGFDFKETSDKVVKTVDQIGDEVTETLK
                     NVFRKAKDSDDRPGKEQFTGSEDYSEPGILKEFIRKTVDATIAKAQGKGLSDSEKQAV
                     DERSRVKILEMLESGKITAEEAERLLKAIGG"
     gene            complement(1516..1875)
                     /locus_tag="PLB85_02495"
     CDS             complement(1516..1875)
                     /locus_tag="PLB85_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOU72225.1"
                     /translation="MEITSCPICKQELAVRELYCPACEVSYRGNFSQSWLGGLNPRQL
                     EFVKLFIIVQGNIKEMEKRLGISYPTVKNRLAEIISVIASESPSDEDFSDILSDLDQG
                     FISVEEAINMIDTRRKS"
     gene            complement(2733..4619)
                     /locus_tag="PLB85_02500"
     CDS             complement(2733..4619)
                     /locus_tag="PLB85_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72226.1"
                     /translation="MRNILSFAAMFLVFVQGWAVTITSTTAGGNWNNPATWGGGQIPT
                     AYDDVVIAGPVKACDTHDCFCNHLTVNAGSFLYGGNGGGSEDGLYVYGNLTNHGTIRD
                     HPLGWGMLLRSYGNITNSGSFTPEILYLHGTADQFISGSGNFSPLNMKDQGSASPVRL
                     LSDLSMAGSRIDLNEGTLDLCGGGSPHTLSLAGGYIYNGFIHGGNGAALALSNGAKLY
                     DLDIDECVFAGTVEIGSAVSVGILRNQGIIQSGNAELPVLTVTQLLENRGTIRNNPAG
                     WYFWLFLSGDLENYGTIANQRTVLNNTQPRYLWQDADANPISCANLEVEVGSAPHELR
                     SNLSFAGGSTNLNGSTLIMYSGRSVHGITFTSGYFGNAVLETDGFSPITGSGVSLYNI
                     QAEDLSFHGTVGIGGNVVIQDLVNYGYIEPRDNPVDITVNGNLVNYGTIRLYVNWTMN
                     LYCRGNISNYGSINNRNVYMDGTADQYLLNAGSISVESFQLVSELGPAQWYFNGSLYS
                     ADVLTNRTINPNTLGVWQPLAGTVWGRLITIGSGLAELDAPGNLQAFLLGTEAKLQWD
                     QVPGAVYYNVYASSDPYALFPADWETPIKAHDPDLADGIVRLDLSGAEPYKFYRVTAG
                     NTMP"
     gene            complement(4753..5442)
                     /locus_tag="PLB85_02505"
     CDS             complement(4753..5442)
                     /locus_tag="PLB85_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72227.1"
                     /translation="MTKPLYIVIGAYGSGKSEFSLHLAKTLNTTEHDVVLADMDVVNP
                     YFRSRDVQEEYSALGIEVIAPEGQFKHADLPMLSPRIKGAVENYAKTVILDVGGDPAG
                     CRTLGRFVEAVTLRGYEMLFVVNTFRPFTSSVEEVLRMKEQLEFASKLKITEFVCNNN
                     LMEHTTPEVVQEGIEILEECSRLTGLPFRRYLVLDAYADIVPDGLGGKERLVMAYTLK
                     KPWEGLALKGI"
     gene            complement(5439..6095)
                     /locus_tag="PLB85_02510"
     CDS             complement(5439..6095)
                     /locus_tag="PLB85_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU72228.1"
                     /translation="MRYKAVIFDLDGTLIDSMGLWRQVDRDFLHKRGIPVPRDLFDHL
                     PQGNSFIQTAQYFKDRFGLADSAEEIMAEWTEMVGWHYANDVKLKPGAGKLVAKLHQA
                     KVPLGLGTSNSRELAEKVLSQNGIWHCFASVVTGDQKLMGKPWPDIYLKGAQELNTAP
                     ADIIVVEDTLTGIQAAKAAGMQAVAIYDSDCEEFHPQIRALADAFVLTHTELSELLES
                     "
BASE COUNT         1233 a         1745 c         1741 g         1550 t
ORIGIN      
        1 aaccggactg cgctacatcc cgaccggttc ggctcaaact gtgctgagcc gggtttctgt
       61 caaggtttta ttgtcagccg ccgatggcct tcagcaggcg ttccgcctcc tccgcggtga
      121 tcttgccgct ttccagcatt tccaggatct tgacgcggct gcgttcgtcc acagcctgtt
      181 tctcgctgtc gctgaggccc ttgccctggg ctttggcgat ggtggcatcc accgttttcc
      241 tgatgaactc tttcaggatg ccgggttccg aatagtcctc gctaccggtg aattgttcct
      301 tcccgggccg gtcgtcgctg tctttcgctt tacggaacac gtttttgagg gtttcggtaa
      361 cctcgtcgcc gatttggtcc accgtcttca ccaccttgtc tgaggtttcc ttgaaatcga
      421 agccttccac ggttttctgg agcttgccca gcgcgccgct gagcttttcc ttgatctttt
      481 cctcgctgat ggagcctatc gccttttcca ccgccgccgt ggctgagttc agggcggccc
      541 tcaccttttc catgtcttct tcgccaaccg cattgccgat cgcgcccttg aggtcattga
      601 ccttcatctt cacgaagttc atgtcgttgt tgccgtcggc gttcagcttg atcacgccgt
      661 tttcggttct tacgctgatc ttgaggccgc cctctcccct gctgatgatt tccctgcgga
      721 ccccgttttc ggtttccccc ctcgacttga gcccgcctgc gccggagtcg ccttcgctgc
      781 cacctgtgac ggaaatgcta cccacgctcg tttcggccgt catttcgaag ttctgctcct
      841 cggccagcac gagtttcacg accccgtttt cgttcacgaa ctccaggtcg ccgctgtcca
      901 ggggcaggct ttcatagaag atcacgccgt tctcgttgct gaccttcgcc cgggcgaaac
      961 tggccaggcg aagcttcacg gggccgtttt cggatttcac ctccagattg gcgccactga
     1021 gcttttccgc agacagggga ccgttttcaa ggcgaatttt gaaatcgccg ctgcagcttt
     1081 tcagcttggt ggagccgttc tcgttcttca gatggatgtg tccttcgcag tcttccagct
     1141 tcagggagcc gttttcgttg tgcagcgcca gcgagccggc gatcccttcc gccctgatgg
     1201 gcaggttgtc agctttggcg atcaccgcgg cgccttgggg aacccgtatc caaagctgag
     1261 agcggcctgt tcccatgaag gattcggcca gttccgggat tctctccaga tagattttca
     1321 aggtgccgcc ttcctcccca acatggaaat tctccgccat gtcggtcccg ggaacgaatg
     1381 aatggatgct gagttcggcc cggatttcca cctggccgga ttcggaacgc ccgatgtgaa
     1441 ggggcgccat tttgttttcg atctccacgg tgtccttgcc cgcgaagtca taatcgagtt
     1501 taatgtttgc cgttttcatg atttcctcct tgtgtctatc atgttgatgg cttcctccac
     1561 ggaaatgaag ccctggtcca gatcgctgag gatgtcgctg aaatcctcat cggacgggct
     1621 ttcgcttgcg ataacgctga ttatctccgc cagccggttc ttcaccgtgg gatacgagat
     1681 gcccaggcgc ttttccatct ctttgatgtt gccctggacg attatgaaca gcttgacgaa
     1741 ttccagctgc cgggggttga gcccgcccag ccagctctgg ctgaaattcc ctctgtaact
     1801 gacttcgcag gccggacagt agagttcccg gaccgcgagt tcctgtttgc agatcggaca
     1861 ggatgtaatt tccatgatct actccttaat aaagtcaagg tcattattaa ttatcctgat
     1921 ttcatgtcaa gatttatttt cattattctc agattatcct tgattacaca ctgttgtttt
     1981 tcttaactca cgggcttggt catgaggtca gcaacaatgc tttggagagc ctgatagggt
     2041 gtttactctg gattttccca ataaatcagc gtgattggcc ctttgcccca tcccactcgc
     2101 ctcctttttg cctcccgtta agttcccgtt tgacgggcgg gaactaagcg ggaagtgaat
     2161 gggatgtgaa tgagatgggg caaaggagga gtcaggattg tggcccgggt tcgtaacttg
     2221 ttgtgcgaca taccggagtg ggatttcgat tgtttcaagc ccgtagggcg gtataacgat
     2281 agcgagggag cgtaagccct tcgtaacaat agcaacaaca tactgcccgc ccataaaccc
     2341 acgtcattcc gggctcgacc tgttcgtagg ggccatggcc cagcacgtat tccacaaatc
     2401 cacgtcattc cgggctcgac ccggaatcca gtttcatcta tctatggatc ccggatcaaa
     2461 gtcacctcgc aagctcggtg acgtccggga tgacgtttgt tcggttcttc cggccaaggt
     2521 cttgcatttc attccaggct tgggatgacg tatgttcggc tctccgggct acccacccgg
     2581 tttgaaagag agccgtttta gctattctta aagccatgat tgtcccgagt ctgggacgca
     2641 aacaccacca ccttccacca caagtggata aacattcaac agcctgttcc ggcaggtatg
     2701 gctgtcaggg ccggtcagaa aacccgttgg gatcagggca tagtgttgcc tgccgtcacc
     2761 cggtagaact tgtacggttc tgctccggac agatcgaggc gcacgatgcc atcagcgaga
     2821 tcgggatcgt gggctttgat gggagtttcc cagtcggcgg gaaaaagggc gtaaggatcg
     2881 gaactggcgt aaacgttgta atagacagcg cccggcacct gatcccactg cagtttggcc
     2941 tcagttccca gcagaaaggc ctgtaagttg cccggagcat ccaactccgc caggccgctg
     3001 cctatggtga tcaggcgtcc ccaaaccgtt cctgccaaag gctgccacac gcccagtgtg
     3061 ttggggttga tagtccggtt ggttaaaaca tccgcgctgt agaggctgcc gttgaaatac
     3121 cactgggcgg gccccagctc cgaaaccaac tggaagcttt caacgctgat gctgccggcg
     3181 ttgaggagat actggtccgc ggttccatcc atatatacat tccggttgtt gatactgccg
     3241 tagttgctga tgtttccgcg gcagtagagg ttcatggtcc agttgacgta tagccggatg
     3301 gtgccgtaat tgaccaggtt accgttgaca gtgatgtcca ccggattgtc acggggctcg
     3361 atgtagccgt agttcaccaa atcctggatg accacgttgc cgccaatccc caccgtgccg
     3421 tgaaagctca gatcctccgc ctggatgtta tacaaagaca ctccggaacc ggtgatgggg
     3481 ctgaagccat cagtttccag gacggcgttg ccgaaataac cgctggtgaa ggtgattccg
     3541 tgcacgctgc ggccgctgta cataatcagg gttgagccgt tgagattcgt gcttcctcct
     3601 gcaaaactga ggttggagcg aagttcgtgg ggggcggagc caacttccac ttccagattg
     3661 gcacaactga tgggattggc atccgcgtcc tgccagagat agcgaggctg ggtgttgtta
     3721 agcaccgtcc tttggttggc gatcgtaccg tagttctcca gatctccgct gagaaacagc
     3781 caaaaatacc agcccgcggg attgttgcgg atggtgccgc ggttttccag aagctgggtg
     3841 accgtaagca ccggcagttc cgcgttcccg ctctggatta tgccctgatt gcgcaggatg
     3901 cccacgctta cagcgctgcc gatctcaact gttcccgcga acacgcattc gtcgatgtca
     3961 agatcataaa gtttcgcccc gttgctgagg gccaaagcgg ctccgttgcc gccgtggatg
     4021 aagccgttat agatatatcc tccggcaagg ctgagggtat gcggactgcc tcctccgcag
     4081 aggtccagag ttccttcgtt cagatcgatc ctggaaccgg ccatactgag gtctgagagc
     4141 agcctcaccg ggcttgccga cccctggtcc ttcatattta gtggcgagaa gttgccgctg
     4201 ccggaaataa actggtccgc agttccatgc aaatacaaaa tttccggcgt gaaagagccg
     4261 gagttggtga tgttgccata actgcggagt aacatccccc agcccaaggg atggtctctg
     4321 atggtgccgt ggttggtcag gttgccgtaa acataaaggc catcctcaga tccgccgccg
     4381 ttgccaccgt agaggaagct accggcgttg actgtcagat ggttgcaaaa gcagtcgtga
     4441 gtgtcacagg ctttcaccgg gccggcgatc actacgtcgt catacgctgt gggaatttgg
     4501 cctccgcccc aggtggcggg attgttccag ttacccccgg ctgtcgtgga ggtaatggtg
     4561 acagcccagc cctgaacaaa gaccaaaaac attgccgcaa aggatagaat gtttctcatg
     4621 accagtctcc ctgtaagatt tgccaactta aataaataaa tccgggatat tagtcaagaa
     4681 aatttccggt taaatcagcc acttgtgtgg agattttgca acagatggtt tgacaggagg
     4741 ttgaacgctg gcttaaatcc ctttcagggc gaggccttcc cagggttttt tgagggtgta
     4801 tgccatcacc aggcgctctt tgccgccgag gccgtcggga acgatatccg cgtaagcgtc
     4861 caaaaccaga tagcgcctga aaggcaggcc ggtgaggcgg ctgcattcct ccagaatttc
     4921 gatgccctcc tgcaccactt ccggggtggt gtgttccatc aggttgttgt tgcagacgaa
     4981 ctccgttatc ttcagtttgg aggcgaattc aagctgttcc ttcatccgca ggacctcttc
     5041 cacgcttgag gtgaaagggc ggaaagtgtt caccacgaag agcatctcat agccgcgcag
     5101 ggtgacagct tcaacgaaac ggcccagagt gcggcagccg gcaggatcgc cgcccacatc
     5161 cagaatcacg gttttggcat agttttccac agcgcctttg atccgcggcg aaagcatggg
     5221 caggtccgcg tgcttgaact ggccttcggg cgcgatgacc tcgataccca gggcgctgta
     5281 ttcttcctgg acgtcgcggg aacggaaata gggattcacc acgtccatat ccgccagaac
     5341 gacatcatgc tcagtagtgt tcagggtttt ggccaggtgg agggaaaatt cgctttttcc
     5401 gctgccatag gctccgatga caatgtaaag gggtttggtc atgattcaag taactcgctt
     5461 agttcagtat gggttagcac aaaggcgtcg gccagggccc ggatttgggg atgaaattcc
     5521 tcacaatccg agtcgtagat ggccacagcc tgcattccgg cggctttggc ggcctggatg
     5581 ccggtgaggg tgtcctccac cactataatg tccgcggggg cggtattaag ttcttgggcg
     5641 cctttcagat agatgtccgg ccagggtttc cccatcagct tctggtcgcc ggtgaccacg
     5701 gaggcaaagc agtgccaaat gccgttctgg gacagcactt tttccgccag ttcacgggaa
     5761 ttgctggtgc cgaggccgag ggggaccttt gcctgatgga gtttggccac cagttttccg
     5821 gcgccgggct tgagcttcac gtcgttggca tagtgccagc caaccatctc ggtccattcg
     5881 gccatgattt cctccgcgct gtcggccaag ccaaagcggt ccttgaaata ctgtgccgtc
     5941 tggatgaatg aattgccctg gggaaggtgg tcgaaaaggt cgcggggaac gggaataccg
     6001 cgtttgtgga ggaaatcgcg gtccacctgg cgccagaggc ccatggaatc tatcagggtg
     6061 ccgtcgaggt cgaagataac agctttatag cgcatttcag ttcagaaaat accagcgcag
     6121 ggaggcctga agcgacccgg gcaggggctc ggcttcgtag atgcgggaat ccaaaaggtt
     6181 tttgtaggtg acctgaaagt cgaagcgcct gccaatcttc actccggccc agatgtcgat
     6241 caccagcgcg tcttcccaaa ccgtccaat
//
LOCUS       DAOOWR010000061         6223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12682_length_6223_cov_3.491245, whole genome shotgun sequence.
ACCESSION   DAOOWR010000061 DAOOWR010000000
VERSION     DAOOWR010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12682_length_6223_cov_3.491245"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLB85_02515"
     CDS             <1..1002
                     /locus_tag="PLB85_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HOU72229.1"
                     /translation="IRNATLTTIAPTGTISMILDTSSGIEPQFSLVYVKNVMDGDKLL
                     YVNKWFRKALEEAGMHSQELLEKVAQAGSIADIEEIPAAIRDIFLTAHDISPEWHIRM
                     QAAFQQYTDNAVSKTINFPNSATVEDIRIAYELAYQLGCKGLTVYRDGSRENQVLCSG
                     SSPQSSERKVAPRSRPEVTHGVTQRLETGCGHMYVTINTDEYGACEVFVQMGKVGGCA
                     SAQLEAIARLSSLALRSDIKLEAIIRQLKGIRCQSPMWNKGRMITSCGDAVGHALETF
                     LATWNGSSLKAVHIVGGEPDILEAGAKTSIALCPDCGSSIEHTEGCLKCPSCGWTKC"
     gene            1037..1810
                     /locus_tag="PLB85_02520"
     CDS             1037..1810
                     /locus_tag="PLB85_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72230.1"
                     /translation="MRCKHCDARLAAHDFWCANCGKQTKVVTQDLSSWNSLTRTWSNY
                     NPLKGLNIPAAAFPVLAGVLPCLALLFALNAFGRLNLSQNQATGSMLLNLFITSAGLS
                     VFLPILLIPYKPACAEPGYTITLKDMLAGMRQYPRYLVLVLISVLFFVIIHLICFGLP
                     QFSSDPILRLVWIVLVNYFLAVFLPVPVLMQRLNLNSWQAAKLSYRKFHVVRWQLFLL
                     GLILVLMNFVATALVLVPLIIILPLSWFAVRDYGSPAGI"
     gene            1842..3647
                     /gene="folE2"
                     /locus_tag="PLB85_02525"
     CDS             1842..3647
                     /gene="folE2"
                     /locus_tag="PLB85_02525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOU72231.1"
                     /translation="MTKKEITSLILLLAVIAYGAWTWFHRSYRDTRLDQDLMDTVVTI
                     SAKSKSKNVGSQIDSVFAYMHTLEAKFNDYDPASWVSKVNSANGKAVPMDPDAYELLC
                     LADSLYQLTDGAFDITIKPLYDLWGFSRPVVQGDSLPLMPPDSLAIKETLKKVGFNRV
                     RFSQKRIIMPRGMQITFGALAKGYVLDKAREFMENDKFISGQIDCTSGMTFFGQPLGQ
                     IVSVRHPRANQQQQTIGNFKIQNGSLSTSGDYQQFFEYENRRYHHILDPKTGYPVENV
                     FSVTVIHPSAAWADGLSTALFLLPPETAIERLKLSRKQRCYLLPVQRPDRFPEEPWHE
                     RIGVARRRMNIPDLQSQTDLRHIGIDKVGVKGIRYPIVVDDRDNKTQSTIGEFNIYVD
                     LHHSKRGTHMSRFLEVLNRYHRDAIIGQLDKLLLELKSLLKADAAYIDIVFPYFLQKR
                     APVSGISSLMDYQCFFNASFAKDYKLWIGAVVPVTALCPCSKEISEAGAHNQRSLVTI
                     KVRYTGLVWLEELICIAEEASSCQVYPLLKRPDEKFVTETAYARPRFVEDIVREVTQK
                     LEDDPRVLEFYVEADSFESIHNHNAYAMVYRPGRD"
     gene            3665..>6223
                     /locus_tag="PLB85_02530"
     CDS             3665..>6223
                     /locus_tag="PLB85_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU72232.1"
                     /translation="MPDFHFQPAVSGFLPNRLKYILSSDASNPVLCLQLYIRVGSAWE
                     TEREAGYSHFMEHLAFKSTRDFGYNQITSFVNGLGGSINAYTDFDCTCYYLLLPSEYL
                     AEGLKVLSELAMHPTFTAEDLAMEKDIIIEEMEQNRNDPEIDFLDFIQASAFKRNPLR
                     LPVMGRAASLKKARLADLKAFHRKYYQPANSFLVVVGDFDRSTAEQALGECFGSWQNT
                     ASLPRTYDGSFSEPETPDQPVVWREHKQKFLTYVLPELCDTHPDSDSLLIALRYVAVG
                     RPSRLFKRLVEEDKLASSVKVSSLSGVMSGVSAIVVSPMGKNNVGRIQSVFQQEYRAL
                     LEGNVDPGEIALVKQDIINTWRYGFDGVENLAGMLGAEEFISGYEELYKYDRQILSLG
                     LEDVLKAVRKYWQPSSLQIIHQSPQSADLSLSVPIIKNSKKSPAKSKTSVAQAVPAPG
                     RAKACLEQYSEDVFTATLANGLKFIYRWQPQRPISGFALSTDLCQLAEEPGQRGLNYL
                     CSAAMLHSTQSRSYAEMLKTCRELGISLNVEPHPDTTVFRGKCFHSALPTALSMLAEI
                     FWQPAFEENHIRLLKSTSIDLLRRDNQNPSSLAWLRWFRLLFGANSPYGRYSGTISDL
                     RSHGREAIRAWYRDYYRPDRFSLSIVSSVEPRKALDLAASLFEGECPAPPSPGFKAPL
                     PQPSHVRLRTQKLDSGQAIIHLGGFAAPASDRTSTTAFHLLAQIIGGDMDSRLFNLVR
                     EKYGYAYQTGMEFSSTNELGYWFTYSYCDPDDRKPCLRLMREVLAAVCADGVGADELL
                     HAQNYLCGMNRFEAESAALQAVLLSSLSALGYEPGFYLNRERRIRSVDLETVNRV"
BASE COUNT         1368 a         1893 c         1572 g         1390 t
ORIGIN      
        1 atccgcaacg caaccctcac caccatcgcc cccaccggca ccatcagcat gatcctggat
       61 acttccagcg gcatcgaacc ccaattctct ctggtctatg taaagaatgt gatggatggt
      121 gacaaactgc tctacgtgaa caaatggttc cgcaaggcct tggaggaagc aggcatgcat
      181 agccaggaac tgctggagaa agtggcgcag gctggcagca tcgctgacat agaagaaatc
      241 cctgccgcca ttcgcgatat cttcctcact gcccacgaca tcagtcccga gtggcacatc
      301 cgcatgcagg ctgcttttca gcaatacacg gacaacgccg taagcaaaac catcaatttc
      361 cccaactccg caaccgtgga ggacatccgc atcgcctacg aactggctta tcagcttggc
      421 tgcaagggcc tgacggtcta tcgggacggc agccgtgaaa accaggtgct gtgcagtgga
      481 agcagtcccc agtccagtga acgcaaggtg gctcctcgta gccggcccga ggttacacat
      541 ggagtcacgc agcgactgga aactggttgc gggcatatgt atgtgaccat caacaccgat
      601 gaatacggag cctgtgaagt gttcgtgcaa atgggcaaag tgggcggctg cgcctccgct
      661 caactggagg caatcgcccg gctttcctcc ctggccctgc gctcggatat taagctggaa
      721 gccatcatcc gccagttgaa aggcatccgc tgccaatcgc ccatgtggaa caaaggcagg
      781 atgataacat cctgcggaga cgctgtgggc catgccttgg agaccttcct tgccacttgg
      841 aatggcagca gcctgaaagc cgtccatatc gtcggaggcg aaccagatat tcttgaggcc
      901 ggtgccaaga cctcgatagc gctctgcccg gattgcggtt cttccatcga acacactgag
      961 ggttgcctga agtgcccttc ctgcggatgg accaaatgct gaccaactct ttaaccttat
     1021 acactaggga gtgaaaatgc gctgtaaaca ctgtgacgcc cgcctggccg cccatgactt
     1081 ttggtgcgcc aactgcggca aacagaccaa ggtggtaact caggatcttt cctcttggaa
     1141 cagcctgaca aggacctgga gcaactacaa tcccctcaaa ggcttgaaca tccccgccgc
     1201 cgcttttccg gtgcttgccg gagtgcttcc ctgcctcgcc ctgctgttcg cgctcaacgc
     1261 ctttggacgc cttaacctct ctcagaatca agcaactggc agtatgctgt taaacctgtt
     1321 tatcacatct gcaggcctat ccgtctttct tccaattcta ttaatcccct ataaaccggc
     1381 ttgtgcggaa ccgggataca caataactct caaggatatg ctggctggaa tgaggcagta
     1441 tccccgttat ctggtcctgg ttcttatatc tgtcctgttt ttcgtgatca tccacctgat
     1501 ttgctttggc ctgccccagt tcagtagtga tcccatcctg cgcctggtct ggatagttct
     1561 ggtaaattac ttcctggctg tgttcctgcc cgtgcctgtg ctgatgcagc gccttaacct
     1621 gaactcctgg caagctgcca agctgtctta ccggaagttc cacgttgtgc gctggcagct
     1681 ctttcttctg ggccttatcc tggtgctgat gaactttgtt gccaccgcgc tggtcttggt
     1741 gccattgata atcatactgc cgctttcctg gttcgctgtg cgggattacg gatctcctgc
     1801 tggaatatga aataatccgg gaatacaaat agggggcagt tttgaccaaa aaggaaatca
     1861 cttccctgat tctgctgctg gccgtgatcg cttacggggc atggacctgg ttccaccgtt
     1921 cctaccgcga caccaggctg gaccaggatt taatggacac cgtggtgacc atctcggcta
     1981 aatccaaaag caagaatgtg ggctcgcaaa ttgactctgt gtttgcctac atgcacaccc
     2041 tggaagccaa attcaacgat tacgaccccg ccagttgggt ctcgaaggtc aattcagcca
     2101 atggtaaagc ggtgccgatg gatcctgacg cctacgagct tctctgtctg gctgacagcc
     2161 tttaccagct taccgacggc gctttcgaca tcaccataaa acccctttac gacctatggg
     2221 gattcagccg tcccgtggtg cagggagatt cgctgcccct gatgccgccg gattctctgg
     2281 cgattaagga aaccttgaaa aaagtgggtt tcaaccgggt gcgcttcagc caaaaacgga
     2341 tcatcatgcc ccgcggcatg cagataactt tcggcgcgct ggccaagggt tatgtgctgg
     2401 acaaggcgcg tgagttcatg gaaaatgata aattcatcag cggtcagatc gactgcacca
     2461 gcggcatgac ctttttcggc cagccacttg gccagatcgt cagtgtgcgc catccccggg
     2521 cgaaccagca gcagcaaacc attggcaact tcaagatcca aaacggttcc ctgagcactt
     2581 ccggggatta ccagcaattt tttgagtatg aaaaccgccg ctatcaccac atcttggacc
     2641 ccaagacagg ttatccggtg gagaatgtct tttccgtgac tgtcatccat ccttccgcgg
     2701 cctgggcgga cggtctctcc acggctcttt tcctgctgcc gcccgagaca gcgatagaaa
     2761 ggctcaagct atcccgaaag caacgctgtt atcttttacc agtccaacgg ccagatcgtt
     2821 tccctgaaga gccttggcat gaaagaattg gagtggcacg acgaaggatg aacatccctg
     2881 acctccagtc tcaaacagac ctacgtcaca ttggcatcga caaggtgggc gtgaagggca
     2941 tccgctaccc catcgtggtg gatgaccgag acaacaaaac ccaatccacc attggcgaat
     3001 tcaacatcta tgtggacctt caccacagca agcgcggcac ccatatgtcc cgctttctgg
     3061 aggtgctgaa ccgctatcac cgcgacgcca tcatcggcca gctcgataaa ctcctgctgg
     3121 agctgaaatc ccttctcaag gccgacgccg cctacatcga catcgttttc ccatatttcc
     3181 tccaaaagcg cgccccggtc tccggaatca gttccctgat ggattaccaa tgctttttca
     3241 acgcttcctt cgcgaaagac tacaagcttt ggatcggggc cgtggtgccg gtgacagcgc
     3301 tctgtccctg ctctaaggag atttcggaag ccggagcaca caaccagcgc tccctggtga
     3361 ccattaaggt gcgctatact ggcttagttt ggctggaaga gttgatttgc atcgccgagg
     3421 aggcttccag ttgccaggtc tatcccctgc tgaagcgccc cgacgaaaag ttcgtcactg
     3481 aaaccgccta cgccaggccc cgcttcgtgg aagacatcgt gcgcgaggtc acccaaaagc
     3541 tggaagatga cccccgcgtg cttgagtttt acgtggaagc ggatagtttt gaatccatcc
     3601 acaaccacaa cgcctacgcg atggtttacc gcccggggcg tgattgagac cggttccgcc
     3661 gattttgcct gattttcatt ttcagcctgc tgtttcgggt tttcttccca atcggctgaa
     3721 gtacatcctg agttcggatg cttccaaccc cgtgctctgc ctgcaactct acatccgggt
     3781 cggcagtgcc tgggagacgg agcgcgaagc cggctattcc catttcatgg aacacctggc
     3841 cttcaaatcc acccgagatt tcggctacaa ccagataaca agcttcgtga acggcctggg
     3901 cggctccatc aacgcctaca cggattttga ctgcacctgc tactatctgc tgctgccctc
     3961 ggagtatttg gcggaaggtt tgaaagtcct gtctgaattg gccatgcatc caactttcac
     4021 tgcggaagac ctggccatgg aaaaagacat catcatcgag gagatggagc agaacaggaa
     4081 cgatcctgaa atagattttc tcgatttcat ccaggcctcc gcctttaagc gcaacccttt
     4141 gcgccttccc gtgatgggca gagcggcctc tttgaaaaaa gcccgcctcg ctgatttaaa
     4201 agcctttcac cgtaaatact accagcctgc gaactccttt ctggtggtcg tgggtgattt
     4261 cgaccgctcc acggcggaac aggctttggg ggaatgcttc ggctcctggc agaacacggc
     4321 ttcgcttccc cgcacttatg acgggagctt ttcagagcca gaaacaccgg accagcctgt
     4381 ggtctggcgc gaacacaagc agaaattcct cacatacgta ttgcccgaac tctgtgacac
     4441 ccatcccgac agcgattccc tgctgatcgc cttgcgctat gtggctgtgg gccgtccctc
     4501 gcggctgttc aaacgccttg tggaggaaga caaactggcc tcttcggtga aggtttcatc
     4561 tctcagcgga gtgatgagcg gtgtttcagc catcgtggtc tcccccatgg gaaaaaacaa
     4621 cgtgggcagg atccagtcgg tctttcagca ggaataccgg gctttgcttg aagggaacgt
     4681 cgatccaggc gaaatcgccc tggtgaagca ggacatcatc aacacctggc gctatggctt
     4741 tgacggcgtg gaaaacctcg ccggaatgct gggagccgag gaattcatct ccggctacga
     4801 agagctgtat aaatacgaca ggcaaatcct gtccctcggt ctggaagacg tgctgaaagc
     4861 cgtccggaaa tactggcagc cctccagtct gcaaatcatc catcaatccc cccaatctgc
     4921 agatttatcc ctgtccgtcc ccataataaa aaactcaaag aaaagccctg ctaaaagcaa
     4981 aacttccgtg gcgcaggccg ttcctgcccc tgggagggcg aaagcctgcc tggaacagta
     5041 ttccgaagat gttttcaccg ccacccttgc caacggcctc aaattcatct accgctggca
     5101 gccccagcgc cccatcagcg gtttcgcgct ttccacggac ctttgccaac tggcggaaga
     5161 gcctggccag aggggcttga attacctctg ctcggcggcg atgctgcatt ccacccagtc
     5221 ccgcagctac gccgagatgc tcaagacctg ccgcgaactg ggtatcagcc taaacgtgga
     5281 gccgcatccg gacaccacgg ttttccgggg caaatgcttt cactccgccc tgcccaccgc
     5341 gctttccatg ctggctgaaa tcttctggca gcctgccttt gaggaaaacc atatccgcct
     5401 gctaaagagc acatccatcg atctcctgcg ccgtgacaac cagaatccct ccagcctggc
     5461 ctggctgcgc tggttccggc tgctctttgg cgccaacagc ccttacggcc gctattccgg
     5521 caccatcagc gatttgcgct cccacggccg cgaagccatc cgcgcctggt atcgggatta
     5581 ttaccggccg gaccggttca gcctgtccat agttagctcc gttgagcctc gaaaggcatt
     5641 ggacctggcc gcttcgctct ttgaaggcga atgccctgcc cctccctctc ccggtttcaa
     5701 agcgcccctg ccccagccca gtcacgtgcg gttgcggacc caaaaactgg attccggcca
     5761 ggccatcatc catctgggcg gattcgctgc tccggcaagc gacagaacct ccaccaccgc
     5821 tttccaccta ctcgcccaaa tcatcggcgg agacatggat tcacgccttt tcaacctggt
     5881 gcgtgaaaaa tacggctacg cataccagac cgggatggaa ttcagcagca ccaacgaact
     5941 gggatactgg ttcacctatt cctattgcga tcccgatgac cgcaaaccct gcctccgcct
     6001 catgcgtgag gttctggccg ctgtatgcgc cgatggcgtt ggcgccgatg aacttctgca
     6061 cgcccagaac tatctctgcg gcatgaaccg tttcgaagcc gaaagcgccg ccctccaggc
     6121 cgtattgttg tccagcctca gcgccctcgg ctatgaacca ggcttttatc tcaaccgcga
     6181 gcggcgcatc aggtcagttg atctggaaac cgtgaaccgc gtc
//
LOCUS       DAOOWR010000062         6130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12932_length_6130_cov_3.292510, whole genome shotgun sequence.
ACCESSION   DAOOWR010000062 DAOOWR010000000
VERSION     DAOOWR010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12932_length_6130_cov_3.292510"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2801)
                     /gene="dnaE"
                     /locus_tag="PLB85_02535"
     CDS             complement(<1..2801)
                     /gene="dnaE"
                     /locus_tag="PLB85_02535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU72233.1"
                     /translation="MSFVHLHNHTQYSLLDGACRVDRMVALAKEYSMPAVAITDHGNL
                     YGVIDFYKQATKAGLKPIIGMEAYIINGELDSEHNKNDNRYHLILLAQNYTGYKNLMM
                     LSSISFIDGFYYKPRISKSLLEKHSEGLICLSACVQGEIASLLANNREDEAREAVKWH
                     QRVFGDRYFLEIQDHGLELEREVMPALIKLAKEMNQPLVLTNDCHYLHKEDNEAHDIL
                     LCIQTAKTFNDPDRMRYNTEQLYFKSPEEMKQLFPEEPEAYANTLKIADMIDLELKYD
                     KFLLPEIETPPEFEDMGCYLRALCEEAAKEKYPEMTDEVRKRIDFELDVIHRMGFDGY
                     YLVVKDLIDNARKQGVPVGPGRGSGAGSIIAYLLDIVQIDPIRYGLLFERFLNPDRIS
                     MPDFDIDFCAQKRGMVIDYIVQKYGRNSVTQIITFSTLGAKSVIKDVARVLMVPATEA
                     NNITKTIPSNKTLEEAYKESPDFRHLIDSNELYQSIYKHSIVLEGLIRQTGIHAAGLV
                     IAPGDLKEYIPLTCSNQKDSDTNILVQYEGKWLDELKFLKMDILGLKTLTIIQKTVDL
                     VRQSHGVELDIDHLDLSDKKVYKLLGNGDTDGVFQFESDGMRKYLIDLKPNQFEDLIA
                     MVALYRPGPMKFIDTYIARKHKREKVVYDHPVMEQILKETYGVTVYQEQVMRIAIEMA
                     KFTRGQADTLRKAMSKKSLDTLMQLKQLFTKGATANGVQSKIADKIWNDWLDFAQYAF
                     NKSHATCYALVAYRTAWLKSHYPVEFMAALLSLEDDPAKVPIKIEVCKAMGIKILPPN
                     LNRSDKEFTVRGKEVMFGLRAIKNIGDAAIDAIVEDRQNNGHYTSIYNFCSRLDSSAV
                     NKTVLESLIASGSMDDLEGSRAQKWAVIEQALSFNTGAQRDKKIGQTSLFDLITTDSD
                     TEEYYPPLPVEEPWSYADQL"
     gene            complement(2955..3893)
                     /locus_tag="PLB85_02540"
     CDS             complement(2955..3893)
                     /locus_tag="PLB85_02540"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HOU72234.1"
                     /translation="MDQRLELLLTAMFISDYDTKGFCVDPEDEYAREALEKFGSFVAH
                     PAVANISRVWDTEIDCSSPPHYALTLTEDIKLDPSFNLDQVRESLVPNADLSRFTADL
                     RGFHRKADFTLHFSQLQSRLAPWLEKLNDLIARRPVQYILEHYLGQEIPTTEVLLCTL
                     TRPFLSVTVSGTRGETLYCLCSRKWLEIAEQKDSLERILISAIWHEFSHHEINPLTFG
                     LYDNLNELNQEQVNWCCALNESIINAITIRLLLREEVISEKHTDWMVKNAERNKASQT
                     RAVLDLLLDYEQNRSRYPDISSIHPRLVEAVGPPPR"
     gene            4081..4884
                     /gene="mutM"
                     /locus_tag="PLB85_02545"
     CDS             4081..4884
                     /gene="mutM"
                     /locus_tag="PLB85_02545"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOU72235.1"
                     /translation="MPELPEVQTVLDGVAKELKGKEILGLDCFYPGTVVKDPELPEKV
                     FPARFLSHHRRGKYMILDLEGGISVIIHLRMTGKLVTDKAMSGISVHERACFLLSGMG
                     KLHFIDIRTFGKIVLCKTGNLGKFMPELGIEPLSDEFDGAHLQKILKGRKTPVKNALL
                     DQRLIAGLGNIYACEILYRAKIDPATPAGRLSLPRLKKLVAETKSVLREAISMNGTSI
                     SDFRRVDDKTGQFQNFLRVYQKDLCPQGHKIAKIKQGARGTFYCPVCQK"
     gene            5280..>6130
                     /locus_tag="PLB85_02550"
     CDS             5280..>6130
                     /locus_tag="PLB85_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72236.1"
                     /translation="MRKMIFMAVLLVCWATVWAGHPARPRLPEAGFDPTPRQFLAALS
                     GTRGMDCLLHQCYEDGWIDTWRQEAIYDSLGRIGCINHYWASNPGDPLELAGRTLFEF
                     SDDGQAQHVTEEYYYEGAWYPFWELLYEYENGMPQEINYNLNMYEGMIHRQTIFIYWP
                     DTNILKRVVEFTYGYESPVPSVIIYDYAMDSASRPSEILVSRIDADWDQWYVQERRNY
                     VYHNDDQTTHESYLRQLEFSYLGTLSCFFVGVQPSKLLEERVFHPGANGTWSERYRWF
                     HSYNSDG"
BASE COUNT         1348 a         1553 c         1677 g         1552 t
ORIGIN      
        1 agttggtcgg catagctcca aggctcttcc accggcaggg gtggataata ttcctcagtg
       61 tcgctgtcgg tggtgataag gtcaaatagt gaggtctggc ctatcttttt gtctctttgc
      121 gctccggtgt tgaaagacag ggcttgttcg atcacagccc atttttgggc tcggctgccc
      181 tccagatcat ccatagagcc gctggcgatc aggctttcca gcacggtctt gttcactgcg
      241 ctactgtcca gccggctgca gaaattgtag atactggtat aatggccatt attctgccgg
      301 tcttccacga tcgcgtcgat ggctgcgtcg cctatgtttt tgatggccct gaggccaaac
      361 atcacctctt tgccgcgcac ggtgaattct ttgtcgctgc ggttcaggtt tggcggcaag
      421 attttgatgc ccatggcttt gcagacttca atcttgatcg gcaccttggc cgggtcgtct
      481 tcaagagaaa gcaacgcggc catgaattcc acaggatagt gagatttcag ccaggccgtt
      541 cggtaagcaa ccagagcgta gcaggtggcg tggcttttgt tgaaggcata ttgagcgaaa
      601 tccagccagt cattccagat cttgtccgca atcttgcttt gcacgccgtt ggcggttgcc
      661 cctttggtaa aaagctgttt aagctgcatc agcgtgtcca ggctcttctt gctcattgct
      721 ttgcgcagcg tgtcggcctg gccccgggtg aatttagcca tctcgatggc aatccgcatc
      781 acctgttcct gataaacggt gacaccatag gtttccttca ggatttgctc catcacggga
      841 tggtcataaa ccaccttttc ccgcttgtgt ttgcgcgcta tataggtatc gatgaatttc
      901 atcgggccgg gccggtaaag cgccaccatg gcaatcaggt cttcaaactg gtttggcttt
      961 aggtcgataa gatatttgcg catgccgtca ctttcaaact ggaaaacgcc gtccgtatct
     1021 ccattgccaa gaagcttgta caccttctta tcgctgaggt caaggtggtc tatgtccagt
     1081 tccacaccgt gggactggcg caccaaatct accgtttttt ggataatggt gagggttttc
     1141 agccccagga tgtccatttt gaggaacttc agttcgtcca gccattttcc ttcgtactgc
     1201 accaggatgt tggtgtcgga atctttctgg ttgctgcagg tgagcggaat gtattctttg
     1261 aggtcgcccg gcgcgatcac cagcccggcg gcgtggatgc cggtctggcg gattaaccct
     1321 tcaagcacga tgctgtgttt gtagatgctt tgatagagct cgttactgtc gatcaggtgc
     1381 cggaaatccg gagattcttt ataggcctcc tctaaagtct tgttggaggg gatggtcttg
     1441 gtgatattat tggcttcagt agcaggcacc atcagcactc tggccacatc cttgatcacg
     1501 gatttggctc ccagggtgct gaaggtgatg atctgggtaa cgctgttgcg gccgtatttc
     1561 tgcacgatgt agtcgatcac catgccgcgt ttctgggcgc agaaatcgat gtcgaaatcc
     1621 ggcatgctga tgcggtccgg attgaggaaa cgctcaaaga gcaggccgta gcggatggga
     1681 tcgatttgca cgatatcaag cagataagcg atgatgctgc cggcgccgga accgcgtccc
     1741 ggacccaccg gaacaccttg tttacgggca ttgtcgatca ggtctttcac caccaggtaa
     1801 tagccatcaa agcccatgcg gtggatcaca tccagctcga aatcaatgcg tttccgcacc
     1861 tcatcagtca tttcaggata cttttctttt gccgcctctt cgcagagcgc gcggagatag
     1921 cagcccatgt cctcaaactc cggcggggtc tcgatttcgg gcagcaggaa cttgtcgtat
     1981 ttcagctcca gatcgatcat gtcggcgatc ttcagcgtgt tggcataggc ctcaggttct
     2041 tccggaaaca gctgtttcat ctcttcgggg cttttgaagt agagctgctc cgtgttatat
     2101 ctcatgcggt ctgggtcgtt gaaggttttt gctgtctgga tgcagagcag gatgtcgtgg
     2161 gcttcgttgt cttccttatg cagatagtgg cagtcgttgg tgagtaccag cggctggttc
     2221 atctctttgg ccagtttgat caaagccggc atcacttcgc gctcaagctc cagcccatgg
     2281 tcctggattt ccagaaaata tcggtcgcca aagaccctct gatgccattt cacggcttcc
     2341 cgggcctcgt cttcccggtt gttggccagt agggaggcga tttcgccctg aacgcaggcg
     2401 ctgaggcaga tcaggccttc gctgtgcttt tccagtagcg atttgctgat ccgtggcttg
     2461 tagtaaaaac cgtcaatgaa agagatggag gagagcatca tcaggttttt gtagccggtg
     2521 tagttctgcg ccagcaaaat caggtggtag cggttgtcgt ttttgttgtg ctcgctgtcc
     2581 agttcgccgt tgatgatgta ggcttccatg cctatgatcg gcttcaaacc agctttggtg
     2641 gcttgtttgt aaaaatctat cactccatac aggttgccgt ggtctgtgat agctacagcc
     2701 ggcatgctgt attccttggc taacgccacc atcctgtcaa cccggcaagc accgtccaac
     2761 aggctgtact gggtgtggtt gtgtaagtgt acaaatgaca tcttggtctc tccctttctc
     2821 tgaaaacata gatttgctgc cgtcggtatt ttgtcaaagg ataatttggc ggggaaggca
     2881 acccgttgca gcacaggagg tcgcgcttac ttgaaagggt cggagatgcc cgggatgccc
     2941 tgtgccagaa gatcctaccg gggtgggggg cctactgcct ccaccagtcg cgggtggatg
     3001 gaagagatgt ctggatacct ggaccggttt tgctcataat cgagcagcag atcaagcacg
     3061 gcgcgggttt gggatgcctt gtttcgttca gcgttcttca ccatccagtc agtgtgtttt
     3121 tcggaaatga cctcctcccg tagcagcaat cggatggtaa tagcgttaat tatgctttcg
     3181 ttcagggcgc agcaccagtt cacctgttct tggttcaatt cattcaggtt atcatacagc
     3241 ccgaaagtga gcgggttgat ttcatggtgg gaaaattcat gccagatcgc tgaaatcagg
     3301 atgcgttcca ggctgtcttt ctgctcagcg atctccagcc atttcctgct gcacagacaa
     3361 tacaaagtct ctccgcgagt ccctgaaaca gtgacggaca aaaatggcct ggtcagagtg
     3421 cacaacaaca cctccgtagt cggaatctcc tgccccagat agtgttccag gatatactga
     3481 accggccttc gggctatcag atcgttcagc ttctccagcc agggcgccag acgcgactga
     3541 agttggctga aatgcagggt gaaatccgct ttacgatgga agccgcgcag gtcagccgtg
     3601 aatctgctca gatcagcgtt tggaaccaga ctttcccgaa cttggtccaa gttgaaggag
     3661 gggtccagct ttatgtcttc ggtaagcgtc aaagcgtaat ggggtgggga gctacagtca
     3721 atttccgtat cccacacccg gctgatattg gccacggctg gatgtgccac gaaactgcca
     3781 aatttctcaa gcgcctcgcg agcatattcg tcttcaggat caacgcagaa acccttggta
     3841 tcatagtccg atataaacat ggccgttaag agcaattcga gtcgttgatc caatctgatc
     3901 ctcatcccca tctccatttt tttgacttta ccttatctaa gcaaaatatg ggtcaccctg
     3961 attctgtcaa caaatatcat cgccttccca ttcaggataa tgaatgattt ttctggctca
     4021 actgcccgga gggattagca tatcagcaaa gaatcattca atcatcagga gatagacgtc
     4081 atgccagaat tgccagaagt gcagactgtt ttggatggag tggctaagga actgaagggc
     4141 aaggagatac ttggactgga ctgtttttat ccgggtacgg tggtgaagga tccggaattg
     4201 cctgaaaagg tgtttccggc cagattcctg tcccaccacc ggcgcgggaa atacatgatc
     4261 ctggatctgg agggcgggat cagcgttatc attcacttgc ggatgacggg aaaactggtc
     4321 acggacaagg ccatgagcgg catttcggtc cacgagcggg cctgtttcct gctctcaggg
     4381 atgggaaagc tccatttcat cgacatccgc actttcggca aaatcgtact ctgcaaaacc
     4441 gggaatctgg gcaaattcat gccggaactt ggaatcgaac cgctctccga tgaattcgac
     4501 ggcgcgcatc tgcagaaaat cctgaagggc cgcaagacac cggtcaaaaa cgcgttgctg
     4561 gaccaaaggc tgatcgccgg gctgggcaac atctacgcct gcgagatcct ctaccgggca
     4621 aagattgatc ccgccacccc ggctggccgg ctcagcctgc cccggctgaa aaagctcgtg
     4681 gcggaaacaa agtccgtact gcgggaagcc atctctatga acggaacctc gatctctgat
     4741 ttccgcaggg tggacgataa gaccggccag ttccagaatt tcctgcgggt ttaccaaaaa
     4801 gacctctgcc cgcagggtca caaaatcgcc aagatcaagc agggagcgcg cgggaccttt
     4861 tactgccccg tttgccagaa gtgaactggg cggaactgcc ttaatcccag cgccgggtat
     4921 cccgaggagg attgaaggca tattgtctag ccgttaaggc cctgtccctt gtcccgggcg
     4981 cgcctctcgc atgatacccg catttgatgc gagcatcatg cgggaggaag gggagaggaa
     5041 cgtaatcggg cgctaaggac ctgactgaca ggacatggcg gtgggtggta atttgtggct
     5101 gtattttgat tcaagtgggt gttggcgccc agccagaaaa cagccctgaa ggctttagcc
     5161 tccataagtg aaaatgagcc aaaaaacact tgacaaataa ctggcggtgg tgagataatg
     5221 ctttcgccgg aagtggtgga tttatgatcc ggtgagacac aacttccaag gagcttgaga
     5281 tgcgcaaaat gatctttatg gcagtattgc ttgtctgttg ggcaacggtt tgggcaggcc
     5341 atccggcaag gccgcggctt cccgaagccg gttttgaccc cactccccgg cagttcctgg
     5401 ccgcgttgag cgggacgcgg ggtatggact gcttgctgca tcagtgttac gaagacggat
     5461 ggatagacac gtggcgccag gaagccattt atgacagcct tggcaggatt ggctgcatca
     5521 atcattattg ggcttcgaac ccgggtgacc cgctggaatt agctggccgg accctctttg
     5581 agttcagcga cgacggccaa gcccagcatg tgaccgaaga atattactat gaaggggcat
     5641 ggtatccatt ctgggaactg ctgtatgaat atgagaacgg tatgccgcaa gagatcaact
     5701 acaacttgaa catgtatgag gggatgatac atcgtcagac catcttcatc tactggcctg
     5761 acaccaacat cctgaaacgg gtggttgaat tcacctatgg ttatgaatca cccgtgccgt
     5821 ccgtaataat atatgactat gctatggatt ctgccagccg gccgagcgaa atattggtat
     5881 ccagaatcga tgccgattgg gatcagtggt atgtacagga acgcaggaac tatgtttacc
     5941 acaatgatga ccagaccacc catgaatcct acctgcgcca gctggaattt tcatacttgg
     6001 ggaccctatc gtgctttttc gtcggggttc aaccctccaa gttgctggag gaaagggtct
     6061 tccaccccgg cgccaatggc acctggagtg agaggtaccg ctggttccac agctacaata
     6121 gcgacgggtt
//
LOCUS       DAOOWR010000063         6124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_12953_length_6124_cov_4.443895, whole genome shotgun sequence.
ACCESSION   DAOOWR010000063 DAOOWR010000000
VERSION     DAOOWR010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_6124_cov_4.443895"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..2486
                     /locus_tag="PLB85_02555"
     CDS             258..2486
                     /locus_tag="PLB85_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOU72237.1"
                     /translation="MNSHARSDSKGPNTGTVNNLVVYIKFSDQTEFSDPRSVYDAKFN
                     AVGDDQFSLRNYFHQVSYDQLDYVSHHFPTCEPNVNLSYTDSHPRAYFLPYNSVTNPT
                     GYRDFQRTDREHQLLADALNSIASQVPSTLNIDADNDGYVDNVCFVIRGPHSAWAELL
                     WAHRWVLYTQDVFINGKMVWDFTFQPENQNSVRTLCHEMFHSVGAPDLYHYDFDGITP
                     AGCWDIMESGGGHMCMHMKYHYGGWLSSIPTIGPGTYTLNPVTSQVNNAYRYELNSQE
                     SLVFEYRKRGADIFEENLPGSGLLIYRVNSTLSGNASGPPDEVYVFRPGGSQTSNGSV
                     FDASFSAQSGRTAFSQHTDPCCFLSYGSSCPVNVHSISEAHETITFTLSTPDAAYPPL
                     ISGFVPADGAIITAVDTLISVQINDLGTNISLVEFFLDDLSVGTSYTQPWSVNIPEGW
                     LTPGQHTVKVRAVNTASLEASAQARVKVVDPLQPTWFGWLSETPVWDEYGRGAVPIKI
                     ALELDLGAQQYLVKKLAFDAVADPWGFPVEPGLVNAKIHRFSNGAITNEILLDIGDII
                     SPMNGRFEHTVNSLIPISGHVAVVLDLFEYQKIRFDINGACGHSWTTEPDRPWTDALA
                     RGVLGAAAIELQLQAPGSPNQDLTVPTPEPSLSAWPNPFAASAVISLDLKAAAPTELA
                     IYNLRGQKVRSLLDSRLGTGRHEIAWDGLDQSGRAVSPGIYFCRLKTGDFAKIIRLAR
                     IR"
     gene            complement(2763..4145)
                     /gene="mnmE"
                     /locus_tag="PLB85_02560"
     CDS             complement(2763..4145)
                     /gene="mnmE"
                     /locus_tag="PLB85_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU72238.1"
                     /translation="MQSAFEPICAQITPPGCAAVNVLRISGSGSIGIVAQFFHPRQKL
                     LKSPSHRVIFGIFHSRDGNPLDQVLCTVFRAPASYTGEDCVEISCHGNPRLAAKILEN
                     LLLEARLARPGEFTLRALLNGKLDLTQAEAVNDLITATGSKAESSALMQVRGLLSQPL
                     KELLEAVNDARLRCELAIDFADQDLPPLDAADLRKRVSAALDKARELHSDGARGRYIR
                     EGIRVCLAGAPNSGKSSLFNAFLCCNRAIVTPHPGTTRDYLEESVSLSGYNLILTDTA
                     GLRENAGPIEREGIDRSLGLMQSADLVLYLLPVDEPTPKADPAALSPVPAKTLWLASK
                     WDLCQPENQGSASLESRDGSTPELALPVSAIAPDGLNALQTAILKRFELPVENPDRPL
                     VTNARHLAALERVIAALTSALTSLDNQAGYEFTAFDLITASSALGEILGVSPDPDLLG
                     RIFADFCIGK"
     gene            complement(4145..6007)
                     /gene="dxs"
                     /locus_tag="PLB85_02565"
     CDS             complement(4145..6007)
                     /gene="dxs"
                     /locus_tag="PLB85_02565"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOU72239.1"
                     /translation="MILEKIKDPSVIKDFNLQELTLLAKEIRKRIVDVVSINGGHIAP
                     SLGTVELTLALLSVFDPIKDRVVWDVGHQSYAWKILTGRNERFDTLRSFGGISGFTNR
                     DESPCDAFSTGHSSTSVSAALGLACARDLQGESGFCIAVIGDGALTGGMSFEALNHSG
                     HLQKNKFLVVLNDNAMSISQNVGGLQEYMARMMASRPYNTLKQQIWDLSGTLPENLRR
                     RFILGAQKLEESMMNILVPNIIFEDLGFKYVGPIDGHDVELVSTMLRRIKRHMVGPVL
                     LHVVTQKGRGYVPAEQDASSFHGVGPFDANSGKQASSGSESWSEVFGKSLVRLAEADP
                     KIVAITAAMAAGTGLASFEKKFPKHFFDVGIAEQHAVTLAAGMACKGVKPFVAIYSTF
                     LQRAIDQVIHDVALPRLPVVLCIDRAGLVGEDGATHQGAFDVSLLSAIPNLIILSPST
                     AEELDSMLAFASKYQDGPVAIRYPRGTASRGELPLTDFVPGRAETLHQGKNIALITDG
                     GARMVAEKTHALLVEAGLNPWLVNLRSLKPLDEALLNELGESCSHLFTFETNAVIGGT
                     GSRIAQLLAAKPCQVVNFGYPDRFIAHGKTEQLNEMIGFTPETLAAGILNRLKA"
BASE COUNT         1232 a         1804 c         1804 g         1284 t
ORIGIN      
        1 cccaccgagg tgacgcagcc tgacggctcc gtgctggatt tgcttgcttc ggcgatgaat
       61 ttgccaaccg cctgcacgac gcgaacggct ataccatcat ccagagcccc atcgacggct
      121 ggtattatta cgctgtccgc tccggaagcg aaccggtccc ttcttcatac agggttggtt
      181 ccgctgaccc aagagcactg ggcctcgagc ccgggataaa catctctcca gagcgttacc
      241 accagaaagt ggcgtttatg aattcccacg cccgttccga ttccaaaggc ccgaacactg
      301 gcactgtgaa caaccttgtg gtctatatta aattcagcga ccagacggaa ttcagcgatc
      361 cccgctcggt ttacgacgcc aagttcaatg ctgtgggcga cgaccagttt tccctgcgca
      421 actactttca ccaggtgagc tatgaccagc ttgattacgt ttcccatcac ttccccactt
      481 gcgaaccaaa cgtcaatcta tcctacacgg acagccatcc ccgggcttac ttcctgccct
      541 acaactccgt caccaatccc accggctacc gcgatttcca gcgcaccgac cgtgaacatc
      601 agttgctggc cgacgccctg aactccattg ccagccaggt gccctccaca ttgaacatcg
      661 acgccgacaa cgatggctat gtggacaacg tctgcttcgt aatccgcgga ccccattccg
      721 cctgggcgga actgctctgg gcccaccgct gggtgctcta tacccaggat gttttcatca
      781 acggcaaaat ggtctgggat ttcaccttcc agccggaaaa ccagaacagc gtgcgcaccc
      841 tctgccatga aatgttccac agcgtgggcg ctccggacct ctaccattac gattttgacg
      901 gcatcacccc cgccggctgc tgggacatca tggaaagcgg cggcggccat atgtgcatgc
      961 atatgaaata ccactacggc ggctggctga gttccatccc caccatcggg cccggaactt
     1021 acaccctaaa ccccgtaact tcccaagtga ataacgctta ccgctatgaa ctcaactccc
     1081 aggaatccct cgttttcgaa taccgcaaac gcggcgctga catcttcgag gaaaacctgc
     1141 ccggttccgg actgctgatc tatcgcgtca attccacact gagtggaaac gcctctggtc
     1201 cgccggacga ggtttacgta ttccgccccg gcggctccca aacctccaac ggctccgtct
     1261 tcgacgccag tttcagcgcc cagagcggac gcaccgcctt cagccaacac acagatccct
     1321 gctgcttcct gagctacgga agctcgtgcc ccgtgaacgt ccatagcatt tcggaagccc
     1381 atgaaaccat cactttcacg ctttcgaccc ctgacgcggc ttatccgccc ctgatttcgg
     1441 gatttgttcc ggctgacggc gccatcatca ccgcggtgga caccctgatt tccgtgcaga
     1501 tcaacgatct ggggacaaac atcagcctgg tcgagttttt cctcgatgac ctaagcgtgg
     1561 gcacatccta cacgcaaccc tggtcggtga atattcccga aggctggctc actccggggc
     1621 agcacacagt taaagtgcgc gctgtcaata ccgccagcct tgaagccagc gcccaggcca
     1681 gggtgaaggt ggtcgatccg ctgcagccga cctggtttgg ctggctaagc gaaactccgg
     1741 tctgggacga atacggacgc ggggctgtgc ctatcaagat agcgctggaa cttgatttgg
     1801 gcgcgcagca gtatctggtg aaaaaactgg ctttcgacgc ggtggccgat ccctggggct
     1861 ttccagtcga gccgggactg gtgaacgcca agatccaccg cttttccaac ggcgccatca
     1921 ctaatgagat actgctcgac atcggcgaca tcatcagccc catgaacggc aggtttgaac
     1981 acaccgtgaa cagccttata cccatctccg ggcacgtggc tgtggttttg gacctttttg
     2041 aatatcaaaa gatccgcttc gacatcaatg gagcctgcgg acacagttgg acaaccgaac
     2101 ctgaccgacc ctggaccgat gcccttgccc gcggcgtttt gggcgcagct gccatcgagc
     2161 ttcagcttca ggctccgggc tctccaaacc aggatttgac cgtccccact ccggaaccaa
     2221 gcctgagcgc ctggcccaat cccttcgctg ccagcgccgt catcagcctg gacctcaaag
     2281 ccgctgcccc cactgaactg gccatctaca acctccgcgg gcaaaaggtc cgttccttgc
     2341 tggattcccg gctcggcacc ggccgccatg aaatcgcctg ggacggcctc gaccaatccg
     2401 gccgcgccgt ttcccccggc atctacttct gccgccttaa aactggagat ttcgccaaaa
     2461 tcatccgcct cgccaggatc agatagaaag ggctcgtttt ggccgtaccc caagtgcttt
     2521 gcccactgcg agttcctgcc ggtcaggccc tgagcgctcg agcctgtcgc tctcccttgc
     2581 ctcccgcata atactcgcat caaatgcggg catgatacgg gaggcacact tgggatatgg
     2641 gacagggcct taacggccag tcagcaaagc atcgcttttc cgcgcgattt ccggctatct
     2701 tatgaccccg ttatcatatg cttccccaaa gggttaactt acccgatttg aaaaagaagc
     2761 aatcactttc cgatgcagaa atcggcaaag attctgccca gaaggtcggg gtccggagaa
     2821 acgcccaaaa tttcgccaag ggcgctggaa gcagtgataa gatcgaaggc ggtgaattcg
     2881 tagccggcct ggttgtcgag ggaggtgagg gcactggtga gggcggctat gacccgctcc
     2941 aaagcggcga ggtgtctggc gttggtcact aggggtcggt cgggattctc cactggaagc
     3001 tcaaagcgtt tcaggatggc ggtttggagg gcattcaaac cgtccggggc tatcgctgaa
     3061 acggggagcg cgagttcagg ggtggagccg tcgcggcttt cgagcgaggc ggagccttgg
     3121 ttttcgggct ggcaaaggtc ccatttgctg gccagccaaa gggtcttggc tggaacgggt
     3181 gaaagcgcgg cagggtcagc cttgggggtg ggttcatcaa ccggcagaag ataaagcacc
     3241 agatcggctg attgcatcag cccgaggctg cggtcaattc cctcccgctc gatggggcct
     3301 gcattttcac gcagtccggc ggtgtcggtg agaatgaggt tgtagccgct cagggaaacg
     3361 ctttcctcca gataatcgcg ggttgtgcca ggatgggggg taacaatggc gcggttgcag
     3421 cacagaaagg cattgaaaag ggaggatttg ccggaattgg gcgctccggc caggcagacc
     3481 cggatccctt cacggatgta gcggccccgg gctccgtcac tgtgaagttc ccgggccttg
     3541 tccagtgcgg ctgagaccct ttttctcagg tcggcggcat caagaggggg gaggtcctgg
     3601 tcggcaaaat cgatggccag ttcgcagcgc aggcgggcgt cattcacggc ttccagaagt
     3661 tctttcaagg gttgggacag aagcccccga acctgcatca gcgcggagct ttctgccttg
     3721 ctgccagtcg ctgttatgag gtcgttcaca gcctcggcct gggtgaggtc gagcttgcca
     3781 ttcagcaaag cgcggagggt gaattccccc ggacgggcca gacgcgcttc cagcagaagg
     3841 ttttccagta ttttggcggc caggcgggga tttccgtggc aggaaatctc cacgcagtct
     3901 tcgccggtgt agctggcggg ggcgcggaaa accgtgcaga ggacctggtc cagcggattg
     3961 ccatcacggc tgtgaaagat gccgaaaatc acgcggtgtg aagggctttt gagcagtttt
     4021 tggcgggggt ggaaaaattg ggcgacgatt ccgatgctac cgctgccgct gatcctcaga
     4081 acattcacgg cggcgcagcc cggaggcgtg atctgggcgc agataggctc gaaagctgac
     4141 tgcatcaggc tttgaggcgg ttaaggattc cggcggccaa agtttccggc gtgaagccga
     4201 tcatctcatt tagctgttcg gtcttgccgt gggcaatgaa tctgtccgga tagccgaaat
     4261 taaccacctg gcagggtttg gctgccagca attgagcgat ccgggagccc gttccgccaa
     4321 tcacggcgtt agtctcgaag gtgaagagat gggaacagct ctcgcccagt tcgttcagca
     4381 atgcctcatc cagcggcttg aggctgcgga ggttgaccag ccaggggttc aggccggctt
     4441 caaccagcaa cgcgtgggtc ttttcggcta ccattctggc accgccgtca gtgataaggg
     4501 cgatgttttt tccctggtgg agtgtttcag cccggcccgg cacgaaatcc gttagtggca
     4561 attctccccg gcttgcggtc ccgcgcgggt agcggatggc aacaggcccg tcctgatatt
     4621 tggaggcgaa agcgagcatg gagtccagtt cctccgctgt ggaaggtgaa agaatgatca
     4681 ggttggggat ggcggagagc aacgaaacat caaaggcgcc ttgatgagtg gccccgtctt
     4741 cgccgaccag cccagcccgg tcgatgcata aaaccacggg cagacggggc agggcaacgt
     4801 catggataac ctggtcgatg gcacgctgca ggaaagtgct gtagatggcc acgaagggtt
     4861 tcacgccctt gcaggccatt cctgctgcca gggtcacggc gtgttgttcg gcgatgccga
     4921 cgtcgaaaaa gtgcttgggg aatttctttt caaaggaggc cagaccggtt ccagcggcca
     4981 tggcggctgt gatggccacg attttgggat cggcttcagc caggcggacc aggcttttgc
     5041 cgaaaacttc gctccagctt tcgctgccgc tgctggcctg tttgccgctg ttggcgtcga
     5101 agggtcccac tccgtggaag gaagaggcat cctgctctgc gggaacgtag ccgcggcctt
     5161 tctgggttac cacgtgcagc agaaccgggc cgaccatgtg gcgcttgatg cggcggagca
     5221 tggtggagac gagttcaacg tcatggccgt cgatgggccc cacatatttg aagccgaggt
     5281 cctcgaagat gatgttcggc accaggatgt tcatcatcga ctcctccagc ttttgggcgc
     5341 cgagaatgaa gcggcggcga aggttttccg gcagggtgcc gctgaggtcc caaatctgct
     5401 gtttgagggt gttgtagggc cggctggcca tcatcctggc catgtattcc tgcaggccgc
     5461 cgacattttg ggagatggac atcgcgttgt cgttgaggac gaccagaaac ttgtttttct
     5521 gcaggtgccc cgagtggttg agggcctcga agctcatgcc gccggtgagg gctccgtcgc
     5581 cgatcacggc gatgcagaag ccgctttcgc cctgcagatc ccgggcgcag gcaaggccca
     5641 gggccgcgga gacggaggtg ctgctgtggc ctgtgctgaa ggcgtcgcag gggctttcat
     5701 cgcggttggt gaagccgctg atgcctccaa aagagcgaag agtgtcaaag cgctcgttgc
     5761 gcccggtgag tatcttccag gcatagctct gatggcccac atcccagacc acgcggtcct
     5821 tgatggggtc aaacacgctg agcagggcga gggtgagttc caccgttccc aggctagggg
     5881 cgatgtgtcc gccgttgatg gaaacaacgt ccacgatgcg tttgcggatt tccttggcca
     5941 gcagggtcaa ttcctgaagg ttgaagtctt ttattacgga tgggtcttta atcttttcca
     6001 atatcatgtt tcacttccgg ttgcgggttt gggcaccgca gttgctggac gcagttcctc
     6061 gcttttcacg aggaccagtt ccagaatcac cagcgccagc gcagcggcca gcaatatctt
     6121 ccag
//
LOCUS       DAOOWR010000064         6098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13035_length_6098_cov_3.350985, whole genome shotgun sequence.
ACCESSION   DAOOWR010000064 DAOOWR010000000
VERSION     DAOOWR010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13035_length_6098_cov_3.350985"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1812)
                     /locus_tag="PLB85_02570"
     CDS             complement(<1..1812)
                     /locus_tag="PLB85_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72240.1"
                     /translation="MKKLIKLYLLLTLTVVPFALAYAQNTTPGTDVRSAVYADHKVME
                     AIDFLKDRLPQLVKDNKAQAVEEYMLHYESLSKLDEADLLYLVGHFYSVVDDPTKAIP
                     YFDVLINDPKLGDDARMMLNLLLYYRAVDYIQNKEKSSAAPFLEDVMNRFSTGKYYPT
                     YMFLWADLLAEDAEEARIQGYVDAYQNSKDWIKNTFVNRRRSVISRLDDLDLESFYAK
                     PDTIKANVLKEQVSHIQRDLQDLYNEFKSLPGLQFTEALNRIAEEEMRLLEDFKAQIR
                     PFESLPPIDLDLLASPDYIGPESAAFNRYREGAILLKQLKDTSVFYGRVLDVMDRFFE
                     RRYELFVNEDESVVGKGFSDMELKRLMDIERNINTYTDVVAEIDQIMAQPNYPSLNID
                     LGPERQEYVEKIADLQNRKERYLAFRKHQDSVEEAIFTELLDEYYATYRDKRSLDELL
                     PEVEDVMLAMIKENYPKDQRGVIEKQYALAHKVGLEDFPIDDTFIANLDFLGLMVDYR
                     RIRYQEQQRLAKVDALGEEQQRAEYQKIVQDKAELLKRYEAFVNANPYFQAMEQPSGG
                     YLLNNAIIYYNMAELQYAVDLEKPEKALANYKKALEIE"
     gene            complement(1974..3131)
                     /locus_tag="PLB85_02575"
     CDS             complement(1974..3131)
                     /locus_tag="PLB85_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72241.1"
                     /translation="MKKVILSVIVLAAVIGIVEAAPFQTMGLLRTPDAYVIPHKSAQL
                     VLAGYYRNVEKPSYVDMDKNGLNFYGMLGVGLFDRVQLDFFGGDYVYFLNAKVKLLQE
                     TPKFPQIAIGMDNIFSPVNRRRAQDYRPYYYWDPEQQVIVHSPIRDSLNLGWIDHPDK
                     TDYEHFSPYIVASKQVVMGGIDWMFNAGFGANRYTGQVPRSRFFSGFFTSVEISPWDN
                     FAIQGEYDGKDFNAGLKYSYKNFGIRLGAEAVEDMAKGSEGNGYENNLRIALGLSYLF
                     DRYSDKNATIRPDLSLYATSIGTEPVVVTTEPVAVAPTESGKPSEVAIVTPGTQLPTP
                     GIVEASAYKELSPEVKDLLAELRLLREERQKAQKALEDLRKWLQELKEENQ"
     gene            3217..3597
                     /locus_tag="PLB85_02580"
     CDS             3217..3597
                     /locus_tag="PLB85_02580"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU72242.1"
                     /translation="MQQQKAKEIFHVGEDLAAKFLANKGYAIVARNFRASTGEIDIIA
                     TRDDLLVFVEVKTRSKHSIKQALMNVSFTKRKRITLTAQRYIIQHPECVKSRIRFDLI
                     VVLYFCHTDSYQIEHLEDAFLPEL"
     gene            complement(4093..5427)
                     /locus_tag="PLB85_02585"
     CDS             complement(4093..5427)
                     /locus_tag="PLB85_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOU72243.1"
                     /translation="MKNTKALLLCLALASLAGTAAADSFTMHVSPQRFLDKDKNTIIN
                     VDYQIPYGNLWFLAQKGGYFAELNLQVEMVVGDQVIYSQTFRDNVGISDKNDARSNKS
                     YLNRLTFSVESEPYLLRLNAKDINSRKTATWTYQVAPLGEQDLLSDLELCSYAKPDSS
                     SFLERFHRGGILYQTQPSLIFDKTESEDLTIYFETYKPEDLADETGLLVLTVSKDSVM
                     VFDQFLDYIPTSSIEGVSLRIPLEKLNPGKYTGSVEFQLGEFSQEKQFEFFLTEPKQE
                     QFCVFTDPEDDFRLLKYFTGATSTSDWKTYDLDKKRRYLSQKWKALAGAGNMNTQTML
                     DQISERVDYANYYYSHFERGWTSDMGRIHIRRGKPDDIEVGTSSDEARFVRKDYQIWK
                     YQGRQKAVYLFLDIQMNGNYRLIYVEGDQRESSNPDYLYYLGDDFDTRKLYN"
     gene            complement(5414..>6098)
                     /locus_tag="PLB85_02590"
     CDS             complement(5414..>6098)
                     /locus_tag="PLB85_02590"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOU72244.1"
                     /translation="FQPAVLSPLPIPVDSCILTQKYSLAADSIHIAGISGARVFNSPP
                     GCYTADLTEQANADSLFGLRLSIYEGNICYEMEPFLYNQWFYYNARYSYKDQIQQLRY
                     IATQNEWKSLRAVPDEKMHEVIDKYWQVHDPSPGTLRNEARESFYKRVITADERFTIH
                     KRLQGWKSDRGRIYIKYGEPDEVHSEVHPLDLHPYIVWVYYNQNLEFIFADTGGFGQY
                     KLRNKDEEY"
BASE COUNT         1429 a         1487 c         1501 g         1681 t
ORIGIN      
        1 ttcgatttcc aaggcttttt tatagttggc aagggctttc tcgggttttt ccagatcaac
       61 cgcgtactgc agttcagcca tattgtaata gataatggca ttgttcagaa gatacccgcc
      121 gctgggctgt tccatggctt ggaaataggg attggcgtta acgaaagcct catacctttt
      181 cagcagttca gccttgtcct gcactatctt ctgatactca gcccgctgct gctcctcacc
      241 cagcgcatct accttggcca gacgctgctg ctcctgatat cggatccttc gataatccac
      301 catcaaaccc aaaaaatcca gattggcaat gaaggtgtca tcgatgggaa aatcctccag
      361 tccaacctta tgagccagcg cgtattgttt ttcgatcacg cctcgttggt ctttgggata
      421 gttttccttg atcatggcaa gcatcacgtc ttcgacttca ggaagtagtt catccaggga
      481 gcgtttgtcg cggtaagtcg cgtagtattc gtctaaaagt tctgtgaaaa tcgcttcttc
      541 aacgctgtcc tggtgcttgc ggaaagccag gtagcgttcc ttgcggtttt gcaggtcggc
      601 gatcttttct acatattcct gccgctcagg gccaagatcg atgttcaggc ttgggtaatt
      661 tggctgagcc atgatttggt cgatctctgc cacaacgtct gtgtatgtgt tgatgttgcg
      721 ttcgatgtcc atgaggcgct tcaactccat gtcgctgaag cccttgccta ccacagattc
      781 atcttcgtta acaaaaagct cgtagcgccg ctcgaaaaag cggtccatta catccaacac
      841 ccttccgtag aatacggatg tatctttcaa ttgttttagc agaatcgcac cttcacggta
      901 acggttgaag gctgcggatt cgggcccgat gtagtcaggt gaggccagca ggtccagatc
      961 gattggaggc agcgattcga aaggccggat ttgggccttg aaatcctcca gcaggcgcat
     1021 ctcctcttcc gcgatcctgt tcaaagcttc ggtaaactgc agtccaggaa gggatttaaa
     1081 ctcgttgtag aggtcctgca aatcacgctg gatatgagag acctgttcct ttaaaacatt
     1141 ggctttgatg gtgtctggtt tcgcgtaaaa cgattccaga tcgagatcat ccaaacggga
     1201 gatcacactc cttcggcggt tgacaaaagt gttttttatc caatctttgc tgttttgata
     1261 ggcgtcaaca tagccttgta tcctggcttc ttcagcatcc tcggccagga ggtcagccca
     1321 taaaaacatg taggtggggt aatatttgcc cgtcgagaaa cgattcatca cgtcttcaag
     1381 gaacggtgcc gctgatgatt tttccttgtt ttggatgtaa tccaccgctc tgtaatagag
     1441 cagcaaattg agcatcatcc tggcgtcatc gccgagcttg gggtcgttga tcaaaacgtc
     1501 gaaatatggg atggctttgg ttgggtcgtc caccacagag taaaaatgcc ccaccagata
     1561 gagaaggtcc gcttcatcga gtttgctcag gctctcataa tgcagcatgt attcttccac
     1621 agcctgagcc ttattatcct tcaccagttg gggaagccta tctttaagga aatctatcgc
     1681 ttccatcact ttgtggtcgg cataaacagc gcttcgcaca tctgttccgg gcgtcgtgtt
     1741 ttgagcatac gccagcgcga aaggaaccac tgtcagcgtc agcaacagat acagtttgat
     1801 gagttttttc ataaccccgc tcgcttaaat cgtcctatgt tatcaatcct gccagttata
     1861 agcaggattc aggatgcatc tggggtgtgc atcctgaatc tcaagcttga ccaagggaaa
     1921 tatgtatgta tcaacctgca cattcaccat gaggtggaat agtgcgaaaa ttattattga
     1981 ttttcttcct tgagttcctg cagccatttg cgcaaatctt ccaaagcctt ttgcgctttc
     2041 tggcgttctt cacgaaggag tcgcagctca gccagcaagt ctttcacttc cggggaaagc
     2101 tctttgtagg cgctggcttc aacaatgcct ggagtgggaa gctgggtgcc gggagttaca
     2161 atggcaacct cagagggttt gccagattca gtgggcgcca cagccacggg ttccgtggta
     2221 acgaccacag gctcggtgcc gatgctggtg gcgtagaggc tcaaatccgg gcgtatcgtg
     2281 gcgttcttgt cagagtatct gtcgaaaagg tatgaaagcc ccagggcgat gcggaggttg
     2341 ttttcatatc cgttgccttc ggagcctttg gccatgtctt caaccgcttc cgcgccaagg
     2401 cggatgccaa aattcttgta ggaatacttg aggccggcat tgaaatcttt accgtcgtat
     2461 tcaccctgaa tggcaaaatt gtcccaaggt gagatctcaa ctgaagtaaa gaaaccactg
     2521 aagaaacgag aacggggaac ctgtccagta tatctgtttg ctccaaatcc cgcgttgaac
     2581 atccagtcga taccgcccat tactacttgc ttggaggcga cgatgtaggg ggagaaatgc
     2641 tcgtagtctg ttttgtcagg atgatcgatc cagcccaaat tcagagaatc acgaattgga
     2701 gaatgtacta tcacctgttg ctctggatcc cagtaatagt aaggtctgta atcctgtgcc
     2761 cggcgccggt tgactggcga gaagatgtta tccatgccaa tagcgatttg agggaacttg
     2821 ggcgtttcct gcaaaagctt gaccttggcg ttgaggaaat aaacataatc accgccgaag
     2881 aaatccagct ggacccggtc gaagagtcct acccccagca taccgtagaa attcagccca
     2941 ttcttgtcca tatcaacgta agaaggcttt tccacgttcc tataatagcc tgccagaacc
     3001 agttgtgccg atttgtgtgg aataacatac gcgtctggcg tgcgcaataa tcccatcgtt
     3061 tggaacggcg cagcttctac gataccgatc acggctgcca gaacaattac actcaatata
     3121 acttttttca tacttccctc cacctttcgg tgttttttct tagtcttggg tcattaatat
     3181 cgttgacaga ttatccgtca agcttttttt tgccgcatgc aacagcaaaa agcaaaagaa
     3241 atattccatg ttggggagga tcttgccgcc aaatttctgg ccaacaaggg ctatgccatc
     3301 gttgccagga atttcagggc gagcactggc gagatcgata tcatcgccac ccgtgacgac
     3361 cttttggtct tcgtggaggt caaaacccgc tccaaacact ctatcaaaca agccttgatg
     3421 aatgtatctt tcaccaagcg caaacgtata acgctaacgg ctcagcgcta tattattcaa
     3481 catcctgaat gtgtcaagtc aagaataagg ttcgatttga tagtggtgtt gtatttttgc
     3541 cacaccgaca gctatcagat tgaacacctt gaagatgcgt ttttgcccga attgtgattt
     3601 atctgaggct ttttgctcat agcggttgtg gaatcggaag ttaaagcgat cggtctgtaa
     3661 atgcgaaatc catagttttc aatcactctt tcaagccgga tgggtaaaaa ccaccggact
     3721 ggtttctggt tgagcgccag tatccactta atattaaaga gagcaataag cccaccgcaa
     3781 tatcctgtca gtcacgccct gagcgctcgc atttattctt ctctcacacc ccacgcaaga
     3841 ttctcgcatc gaatgcggct attatgcggg aggctctttt gggccccggg acaaggcctt
     3901 aacggccaga tggcatcgct tcttacaact gcttcacagc ctgctgtata tgctgaccta
     3961 cgggattgaa gccatttgat tacctactgg atttgagaga gatctgtttt cagattaact
     4021 ggcagataac aaacccttcc tccaactcgg cttccatatt ggatattgta agcataacga
     4081 ctatccggcc agtcaattgt agagttttct ggtgtcgaag tcgtccccca ggtaatagag
     4141 gtaatccgga ttcgaacttt ccctctgatc accctcgacg tagataaggc ggtaattgcc
     4201 gttcatctga atgtccagga agaggtaaac cgctttttgc ctgccttggt atttccatat
     4261 ctgatagtct ttgcgaacga agcgggcttc atcagaagag gtgcctactt ctatatcatc
     4321 gggtttgccc ctgcggatgt ggatgcggcc catgtcagag gtccagcccc gctcaaaatg
     4381 gctataatag tagttcgcgt agtcaaccct ttcgctgatc tgatcgagca tggtttgggt
     4441 gttcatattg cctgctccgg ccagggcttt ccatttttga ctcaggtatc ttcgcttttt
     4501 atcaagatca taggttttcc aatcgctggt cgaggtagct cctgtgaagt atttgagcag
     4561 cctgaaatca tcctcgggat cggtaaaaac acagaactgc tcctgcttgg gctctgtcag
     4621 gaaaaactcg aactgttttt cctggctgaa ctccccaagt tgaaattcca ccgatcccgt
     4681 gtatttgcct gggttcaact tttccagagg gatgcgcagg gaaactcctt cgatgctgga
     4741 ggtcggtatg tagtccagaa actggtcaaa caccatcacg ctgtcttttg atacggtcag
     4801 aaccagcagc ccggtttcgt cagccaggtc ttccggcttg taagtctcaa aatagatggt
     4861 gaggtcttcg ctctccgttt tatcgaagat cagcgatggc tgggtctggt agaggattcc
     4921 gccacggtga aaccgctcca gaaaactgct gctgtcaggc ttcgcgtaac tgcataattc
     4981 caagtcgctg agcagatcct gttcgcccag tggagcgacc tggtaggtcc aggtggcggt
     5041 ttttcttgaa ttgatgtctt tcgcgttcag gcgcagaagg tagggctcgc tttccacgct
     5101 gaaggtcaac cggttgagat agcttttgtt ggagcgggcg tcgtttttat ccgagattcc
     5161 aacattgtcg cggaaggttt ggctatagat aacttgatcg ccaaccacca tctcaacctg
     5221 caggttcaac tcagcaaaat aaccgccttt ttgggcaagg aaccaaagat ttccgtaagg
     5281 gatctggtaa tcgacgttga taatggtgtt tttatccttg tccaaaaagc gctgcgggga
     5341 cacatgcatg gtgaaagagt cagccgcggc cgttcctgcc aggctggcca aggccagaca
     5401 gagcagtaat gctttagtat tcttcatctt tgttccttag tttgtattgg ccaaaaccac
     5461 cggtgtcggc gaagataaac tccagattct ggttataata gacccagaca atatatggat
     5521 gcaggtccag gggatgcact tcgctgtgca cctcatccgg ttcgccgtat ttgatgtaga
     5581 ttctacctct gtcgctcttc caaccctgca gccttttatg gatggtgaag cgttcgtcgg
     5641 ctgtgatcac gcgcttgtaa aaggattcgc gtgcttcgtt gcgcagggtt cccgggcttg
     5701 gatcgtgtac ctgccagtat ttgtctataa cttcgtgcat tttctcatcc ggaacggccc
     5761 gcaggctttt ccattcattt tgtgtggcga tatagcgcag ttgctggatc tggtctttat
     5821 agctgtagcg cgcgttataa taaaaccact ggttgtagag gaagggctcc atttcgtagc
     5881 agatgttgcc ctcgtagatg ctaaggcgta agccgaacag ggaatcagca ttggcctgtt
     5941 cggtcagatc ggccgtgtaa cagcctggcg gtgaattgaa tacgcgcgca ccagaaatac
     6001 ccgcgatgtg gatgctatcc gcggccaggc tgtatttttg ggtgagaata cagctatcca
     6061 ccgggattgg cagaggactt aaaaccgcgg gttggaaa
//
LOCUS       DAOOWR010000065         6017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13312_length_6017_cov_3.143408, whole genome shotgun sequence.
ACCESSION   DAOOWR010000065 DAOOWR010000000
VERSION     DAOOWR010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13312_length_6017_cov_3.143408"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1128
                     /gene="amrS"
                     /locus_tag="PLB85_02595"
     CDS             130..1128
                     /gene="amrS"
                     /locus_tag="PLB85_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU72245.1"
                     /translation="MRETNEHECMHFRKMEGQAVRCLLCPHCCLLEVGESGLCRGRKN
                     IGGKLIAVNYARSIGMALDPIEKKPLYHFRPGSKIVSLGSNSCNLSCFFCQNSESSQQ
                     GCPTIIISPMELKELVLRKCGDGPLQVAFTYTEPLTWYEYVYDFARQAPEVDIVLVTN
                     GFINPKPLDELLPHIKAMNIDLKSIRPDFYEKHCEARLEPVLKSIERVMGEGVHLELT
                     NLLIPGLNDSEAEIKELVDYVASLDRRIPLHFSAYHPAFRSQIPSTPESTVLQACRIA
                     SGKLDYVYAGNILSAEFHATLCPKCSSEVISARREPLALKAGGCCSHCSQPIYGVF"
     gene            1128..1493
                     /locus_tag="PLB85_02600"
     CDS             1128..1493
                     /locus_tag="PLB85_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOU72246.1"
                     /translation="MIRRLREYMSLADFLLILAVLAAIIASAFIFIKKEDQRRVYIYK
                     DNLAFGSYPLNQDRVVRIDEHNTVEIKNSKVRMLSADCPDHRCVKQGAGDVLPIVCLP
                     NRVVVEIRSRSAERSLIVQ"
     gene            1504..3468
                     /locus_tag="PLB85_02605"
     CDS             1504..3468
                     /locus_tag="PLB85_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72247.1"
                     /translation="MLNFAIYVSDHGFGHATRMAALAEQFIRFGIFTHIRSARPEFLW
                     RTLDPNYSEIHPQACDVGVKHGKNLGIDLEATRTALLDLMGRRLEIVEGEVDFLRREK
                     IDLIIADIPWLSVEAGTYADVPVFAISNFDWLFIYERLFHGLKDMRPVLNTIFGLYQR
                     VDRAFRLPLSSPQSMGSFRKIEKTGLLALKSKPNPDLKKDIGIDSEKPLLVCSFGGAG
                     EMEIELKKLCAAFDGTVASTLDQTGGSNHFRVSQDDDFSALISVADILLTKPGYGSFA
                     EAIQNGKHLIFSPRTNYPEEDVLIRGIASYPAKTELPHLLLSKAEWKSVFGTIRDQKP
                     AKKVPNANAAIASLIIQRFIEQKHGGKKLISVFDVGSNNLNYALCESGQAVPIHTAQI
                     KTGLGQKYHEMQDGRVIIPTRQIAEFKRSVSAFMRFDRDIASRKQVLATGIHRRGDFR
                     DDLAAWFQKRWNAPYKVLNGWEEAELAALAALDLIPQDRQAVIIDIGGFSTEIVSKGD
                     NNKPPMTSLPLGLLTLNQAQQEGVDVKKLIRRHLKEVPDTKPDLVICVGLSAVFLAKI
                     IKQIPKYQPVKIHGLTISRQELEEFKNLAAGDETDEIQRWSVEKQSLPFLRLSALFYT
                     LLLDKFCASEFIVCHHGISSGYNLWKKHKR"
     gene            3473..4747
                     /gene="miaB"
                     /locus_tag="PLB85_02610"
     CDS             3473..4747
                     /gene="miaB"
                     /locus_tag="PLB85_02610"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU72248.1"
                     /translation="MKFYIETYGCQMNVSDSELVSSILIGAGWEIASSIDTANLILFN
                     TCSVRKHAEDRVLGRISNELHRKSQNPELKIAVIGCMAQRMGQRLMENDIGVDFVVGV
                     DQYQQLPALLNQDSGVETEFNYQQIYPGLRPLHGDKTCAFVTIMRGCDNFCSYCIVPR
                     VRGRERSVDFQQIVSEVRECGNKGLKDVTLLGQNVNSYSWGDFDFPRLLKDLNKIESI
                     YRLHFITSHPKDLSDSLIETMAGSDKVCEHLHLPLQSGNNSVLKAMNRKYTIEEYLKL
                     VDKLRTSIPGIALTTDLIAGFPGESEADFADTLDVMSEVHFDYAFCFKYSEREGTSAC
                     NLPGQIPETERLKRLQKMIDLQRKITLAKFSAQVGKTVEVYVESISKKSDKMVSGKTR
                     DYKIAVLSGSVNDIGKLKTAKVIKATPGTLIC"
     gene            4865..5740
                     /locus_tag="PLB85_02615"
     CDS             4865..5740
                     /locus_tag="PLB85_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOU72249.1"
                     /translation="MKSMTGYGVAKVHSNDIDLEIELKSVNGRYLDLRLYLPREISFF
                     EHILRKQIAATLKRGTVEARINFTDHREPRLLLNKAKLEKYHAIVEEAASVLGMTGQV
                     SLEFLLNEPGIIENANNLDEDRVLNEMLHNTLEKALANLVKSLSAEADNITQVLSDSC
                     EHMLEALSAIEAQIKPYKEELFESMKTRTAELLNSFCTENLEQRLIQELAIYIDRYDI
                     QEEISRLRSHISTLLSAIEKDEDNGKTLNFILQEMQREANTLGSKFSTTKTYTHILIL
                     KEEVEKCREIVQNVA"
     gene            5772..>6017
                     /locus_tag="PLB85_02620"
     CDS             5772..>6017
                     /locus_tag="PLB85_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72250.1"
                     /translation="MERPKASALLLAFLIFLAWFLWNLAVKQWQQPWFYFLGMLLVLG
                     NLLPYFIPTTYYLFEYEIVIYYLFLKIARRYEYFGCFY"
BASE COUNT         1587 a         1492 c         1491 g         1447 t
ORIGIN      
        1 acgtctgcct gtcacgcaga aggccgcgag ttcgagtctc gtcgctcccg ccataatact
       61 cggatgccgg aaacggcatc tttttttttg agggagagcg gatccctcct gaccgggaga
      121 cctgaacgaa tgagagagac aaacgaacac gagtgcatgc atttccgaaa aatggaaggc
      181 caggctgtgc gctgtttgct ttgcccgcat tgctgccttc tggaggtggg ggagagtggg
      241 ctttgccgcg ggcgcaagaa catcggcggc aagctgatag cggtcaatta tgcccgcagc
      301 atcggaatgg cgctcgatcc catcgaaaag aagcctctct atcatttccg ccccggttcg
      361 aaaatcgtat ccttgggttc aaattcctgc aacctaagct gctttttctg ccagaactcc
      421 gaaagcagcc agcaaggatg cccaaccatt attattagcc cgatggaact gaaggaactg
      481 gtgctgagga aatgcgggga cgggccactg caggttgcgt tcacctatac agaaccgctt
      541 acctggtatg aatatgttta cgatttcgcg cgccaggcgc cagaggttga catcgtgctg
      601 gtgaccaacg gtttcatcaa tccgaaaccc ctggatgaac tgttgcccca tatcaaagcg
      661 atgaatatag acctcaaatc gattcgcccg gatttttacg aaaaacactg tgaagcacgc
      721 ctggagcctg ttctgaagag cattgagagg gtgatggggg agggcgtgca tctggaactg
      781 accaacctgc tgattcccgg gttgaacgat tcagaagccg agatcaaaga actggtggat
      841 tatgtggcaa gcctggacag gaggataccg ctgcattttt cagcttatca tcccgctttc
      901 aggtctcaaa tcccgtcaac cccggaaagc acggttcttc aggcttgccg gatcgcctca
      961 ggcaagctgg attatgttta tgccggcaac atcctttccg ctgagttcca cgccaccctc
     1021 tgcccgaaat gctcttcgga agttatttcc gcgcgcagag agccgcttgc cctgaaagcc
     1081 ggcggatgct gttcccactg ctcccagccg atctatgggg tgttttgatg atacgtcgcc
     1141 tgcgggaata tatgagcctc gcagattttc tgctgatcct ggctgttctg gcagccatca
     1201 ttgccagtgc cttcattttc ataaagaaag aggatcagcg acgggtttac atatataagg
     1261 ataacttggc ctttggctcc tatcctctga accaggaccg cgttgtccgc atagacgagc
     1321 acaacacggt ggagatcaaa aacagcaaag tgcggatgct gtcagcagat tgccctgacc
     1381 atcgctgcgt aaaacaggga gctggagacg tgcttccgat tgtttgcctg ccaaaccggg
     1441 tggtggtgga aatccgctcc cggagcgctg agcgcagcct gatagtccag tgaggcatac
     1501 ttaatgctca atttcgccat ttacgtttcc gaccacggct ttggacatgc aacccggatg
     1561 gccgctctgg cggagcaatt tatccgcttc ggcatcttca cgcacatccg cagcgccagg
     1621 ccggaattcc tctggcgcac tcttgaccca aattacagcg aaatccaccc ccaggcctgt
     1681 gatgttggtg ttaagcacgg aaaaaacctc ggcatcgatc tggaggcaac ccggaccgct
     1741 ctgttggact tgatggggag aaggctggaa attgtagaag gggaggtgga ttttctgcgc
     1801 agggaaaaga tcgacctgat aattgccgac attccctggc tgtctgtgga agctggcacc
     1861 tacgcagatg tcccggtttt cgccatttcc aatttcgact ggctgtttat ttacgagcgc
     1921 ctgttccatg gtctcaagga tatgaggccg gtgctgaaca ccatctttgg tctctatcag
     1981 agggtggacc gcgcttttcg gctgccgctg agctcgcctc agagcatggg gagtttccgc
     2041 aaaatagaaa aaaccgggct gttagctctc aaaagcaagc caaaccctga tttgaaaaag
     2101 gatattggca ttgattcaga gaagcccctt ttggtatgtt cctttggagg ggcgggggag
     2161 atggagatcg aactgaagaa gctttgcgcc gctttcgatg gaaccgttgc cagcaccttg
     2221 gaccagactg ggggatctaa ccatttccgg gtcagccagg atgatgattt ttccgccctg
     2281 atttccgtgg cggacatact gctgaccaaa cccggttatg gcagcttcgc ggaagccatt
     2341 caaaacggaa aacacctcat cttcagcccc cgcaccaact accccgagga ggatgtcctg
     2401 atccgaggca tcgccagcta tccagccaaa actgaattgc cgcatttgct gctttccaag
     2461 gctgagtgga aaagcgtttt tggaacaatt cgagaccaga agccggcaaa aaaggttccc
     2521 aatgccaatg ctgccatcgc atcgctgatc atccaacgct tcatagaaca aaagcatggc
     2581 ggcaagaagc tgatttcggt ctttgatgtg gggtccaaca atctaaacta cgcgctctgc
     2641 gaatccgggc aggcggtgcc catccataca gcgcagataa aaactggcct ggggcaaaaa
     2701 tatcatgaga tgcaggacgg acgtgtgata attcctacca ggcaaattgc agagttcaag
     2761 cgctcggttt ctgcttttat gcgttttgat cgtgacattg cctccagaaa acaggtgctc
     2821 gcaacaggaa tccaccgccg gggtgatttc agagatgacc ttgctgcctg gtttcaaaag
     2881 cgttggaacg caccctacaa agtgctcaac ggctgggaag aagctgaact ggcagccctg
     2941 gcagccctgg atttaattcc acaggacagg caggctgtca tcatcgatat tggtggtttt
     3001 tcaaccgaga tagtttccaa gggtgacaat aataaaccac cgatgacaag cttgccgtta
     3061 ggattgctta ctctgaacca agcccagcaa gaaggagttg acgttaagaa gctgatccgc
     3121 cgccacttga aagaggttcc ggacaccaaa ccggacctcg tgatttgcgt ggggctgtct
     3181 gctgttttct tggccaagat aatcaaacag atacccaaat accaacccgt gaaaatccac
     3241 ggcttgacca tttccagaca ggaactggag gagttcaaaa atcttgccgc tggggacgaa
     3301 acagacgaaa tacaacgctg gtctgtggaa aagcaatccc ttccttttct aagactcagc
     3361 gccctctttt acacgcttct gctggacaaa ttctgcgctt cggaatttat tgtttgccat
     3421 catggaatat caagcggata caatctttgg aagaaacaca agcgctaaag gaatgaagtt
     3481 ttacattgaa acctacggct gccagatgaa cgtatcggac agcgaattgg tcagttctat
     3541 actgattggc gccggatggg agattgcttc cagtattgac actgccaacc tcatcctctt
     3601 caacacctgc tctgtgcgca agcacgccga agacagggtt ttggggcgta tttccaatga
     3661 attgcaccgc aaatcacaaa acccagaact gaaaatcgcc gtgatcggct gcatggctca
     3721 aagaatgggg cagaggctga tggaaaacga tatcggagtg gactttgtgg ttggggtgga
     3781 tcaatatcaa cagcttccag ctttgttgaa tcaagacagc ggagtggaaa cggaattcaa
     3841 ctatcagcag atctatcccg gtcttcgtcc cttgcatggt gacaagacct gcgctttcgt
     3901 taccatcatg cgaggctgcg acaatttctg ttcctactgc atcgtgccac gcgtacgggg
     3961 acgtgaacga agcgtggatt tccagcaaat cgtttcagaa gttcgtgaat gcggtaacaa
     4021 gggattgaaa gacgttactt tacttgggca gaatgtcaat tcctatagtt ggggtgattt
     4081 cgactttcca cgtttgctga aggatctgaa caagattgaa tccatatata ggctccattt
     4141 catcacttcc catcccaaag acctttcaga ttctctaatt gagactatgg ccggcagcga
     4201 taaagtttgt gagcatctcc atctgccttt gcagagcggc aacaactctg ttctgaaagc
     4261 catgaaccgc aagtacacga tcgaggaata cttgaagctg gtggacaaac tgagaacttc
     4321 catacccgga attgccctta ccacagacct tatagccggc tttcccggtg aaagcgaagc
     4381 tgatttcgct gatactctgg atgttatgag tgaagtacat tttgactatg ccttttgttt
     4441 caaatacagc gaacgcgaag gcacatccgc ctgcaatttg ccaggccaaa taccggaaac
     4501 ggaacgactg aaacgcctgc aaaagatgat cgatctgcaa cgcaaaatca ccctggctaa
     4561 gttttccgcc caagttggca aaactgtgga ggtctatgtg gaaagcatca gcaaaaaatc
     4621 tgataaaatg gtttccggca aaacccgcga ttacaagatc gccgttctga gcgggagtgt
     4681 caatgacatc ggcaaactta aaacggccaa agtgatcaaa gccacaccag ggactctgat
     4741 atgctaatga cccacggcct atatgaacat actgataaga catgttatgt aaacttgcat
     4801 gcagagaggt taattcgttg agattcagaa cactctcccc cacgatattg ataaggaatg
     4861 taatatgaaa agcatgacag gctatggagt tgccaaggtt cattccaatg acatcgatct
     4921 ggaaatcgaa ctgaaatccg tcaacggaag atatcttgac ctgaggctct acctaccgcg
     4981 tgaaattagt tttttcgaac acatccttcg caaacaaata gcagctactc ttaaacgggg
     5041 aaccgtcgaa gccaggatca atttcaccga ccatcgcgaa cctcgtctcc tgcttaacaa
     5101 agcaaagctt gaaaaatatc atgcgattgt tgaagaggca gcctccgtct tgggcatgac
     5161 tggacaggta tcccttgaat ttctgttgaa tgaaccaggg atcattgaaa acgccaataa
     5221 ccttgatgag gacagggtac ttaatgaaat gcttcacaat accttggaaa aagcgttggc
     5281 gaacctcgta aagtcccttt cagccgaagc agataacatc acccaggtgc ttagcgattc
     5341 ctgtgaacat atgctggaag ccctatctgc catcgaggca cagataaaac catataagga
     5401 agaactgttc gagagcatga aaacacgaac tgctgaactg cttaattctt tctgtacaga
     5461 aaatctggag cagaggctga ttcaggaact ggctatctat atagacagat atgacatcca
     5521 ggaagagatt tcccgcctgc gcagccatat ctccactctg ctaagcgcaa ttgaaaagga
     5581 cgaggataac ggcaagacat tgaatttcat tctgcaagaa atgcagcgtg aagcaaatac
     5641 cttaggttcg aagttctcta caaccaaaac ctacacccat atcctcatcc tcaaagaaga
     5701 ggtggaaaaa tgccgggaga ttgtacagaa tgtcgcctga accattatta acatggcgct
     5761 cctggccatt tgtggagcga ccgaaggctt ctgccctgtt gctggccttt ctcatatttc
     5821 tggcttggtt tctctggaat ctggctgtaa aacaatggca acagccttgg ttctattttc
     5881 tggggatgtt gcttgttttg ggcaaccttc tcccctactt cattccaact acctattatc
     5941 tgtttgaata tgagatagtt atctactatc tgtttctaaa gatcgcgcgc cgttatgagt
     6001 atttcggctg tttttac
//
LOCUS       DAOOWR010000066         5998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13360_length_5998_cov_4.507320, whole genome shotgun sequence.
ACCESSION   DAOOWR010000066 DAOOWR010000000
VERSION     DAOOWR010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13360_length_5998_cov_4.507320"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLB85_02625"
     CDS             complement(<1..485)
                     /locus_tag="PLB85_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU72251.1"
                     /translation="MKAITIIGAGLAGSEAALQLADRGLAVKLCEMRPGKMTPAHQTG
                     LAAELVCSNSLKSTLPDTPAGMLKEELRLLGSKLLPIAESCSVPAGHALAVDRELFAQ
                     KVDTLVRTHPKIEMVHEEVTRLPESVCILCSGPLTSDALAAELQKILGSGQLCFFDAI
                     AP"
     gene            complement(493..1377)
                     /locus_tag="PLB85_02630"
     CDS             complement(493..1377)
                     /locus_tag="PLB85_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU72252.1"
                     /translation="MDATTRKKVWRVLSFALKLLVTALILYGLFRRMDFGKVWQNLLN
                     QPVWLILAILGLAALRHWWQCLAWQYSLQINPLFVLKRKEVFESYMIGQALRFAVPGS
                     WGIMGKVAYVSNSSKAASMISCMLERIYVTWSIFVFASLALLYLPHGIPAWARWLLFI
                     GLLTLPVWLYFVTYLNQRWARLRAGYIRYAPRLTLLMIGATLINHFQYWLMLNLNQAV
                     PFWDVVKRMSLSHLSYSIPVTVAGLGLKESFAISLLSKIGLQAESIASTILTIFIIND
                     VVPALIGAGIFLRMKGIR"
     gene            complement(1379..2836)
                     /locus_tag="PLB85_02635"
     CDS             complement(1379..2836)
                     /locus_tag="PLB85_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOU72253.1"
                     /translation="MEKLRGDLANLQAYFSANYAPEQRSAAQTAFLKKLSELTHAHYR
                     GKMQTLPKAGIWDFNWFNVWYTPGVSAVSTSIRDGYERSYELSNRGNLVAVVSDSTRV
                     LGDGDCGPAGGLGVMEGKAMLMKYLGGFDAIALCVNNRDATGKPNPERLIEFVKMLEP
                     SVGAVNLEDISQPNCYRVLDELRASCGIPVWHDDAQGTACVTLAGLLNALKLAGKSIQ
                     SIRAVLFGAGASNNTIASILIQSGLDPQNLVMFDSRGGLHAGRDDLAQNPAKSRQWAL
                     ARISNPKRLATMEEAMKGADVLISLSTPGPHVIKPEWIRLMAPKAIVFACANPVPEIY
                     PHDAKAAGAFIVATGRGDFPNQINNSCGFPGILKGALLVRASQITDGMAIRAAHSLAD
                     FAEAREINPDNIVPRMDEENVFANEAADVAEQAVREGVAAQPLTRDEVFARAAADIRA
                     TRDLCHRMMSDGSINPPPRELIETALKHTRELLGK"
     gene            3058..4191
                     /locus_tag="PLB85_02640"
     CDS             3058..4191
                     /locus_tag="PLB85_02640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72254.1"
                     /translation="MKICIVSNSHHTTDVRLYYKMARSLARLGEVHLLCASGVRNAAV
                     NPYQDVVETESAWYALPLLYKKAKKLRPDIVICVEPLTVCVGLVLRRKLRCRVVFDVH
                     EFFPDAYAERFHPPFSWIVKALYLAFERQLQRRVDATIAVNRKILDQLVPHGKGKNTL
                     VLPNYPVKNVWDYTCEIPMELSAICEMDFDLIYIGGLTSDRGIFKLLKSVYLLRNEFP
                     GLNVLILGRFFSPEVEREFNERLNALGLNAVIYYQSWIPAEKIGLLLKRSRVGLWLFN
                     PQNRRLRRALPLKVLEYFAAGLPVVSINTPLMRGLIESNRLGACCEYQSDSIAEAIAG
                     LLRLGRDEYSQLSQRCVELIDRKYNWEAVEPRLLDLMRKLGPS"
     gene            4193..5446
                     /locus_tag="PLB85_02645"
     CDS             4193..5446
                     /locus_tag="PLB85_02645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72255.1"
                     /translation="MRVLYISYFYPPLGGPAALRNLKTVKYLAEAGIVCDVITVKDIE
                     YLYRDPALMEENAAATLIRADSLDPMALLKKGRNAGGSELSQVYLNTPEKLKLKVRQL
                     WPVDNKIGWLPALIKAGRRAIRGRKYDLIYISLGPFSSGFGALTLSRSSGIPLAVDLR
                     DYWTLLGDYDLQSGIFKKLSRGLEKSIYRHSSLIVTATEGIGTDAAEAFGAELAEKTV
                     TVYNGWDEADFSALPEPIPSSGFHIAYFGNIYARRSLRHFYAALRRLREENALPAGTR
                     VKLYGNFFRETLREVEQSGIQDLIDIIPQLSHREALVAMRNSSALILVINSSSPRGTL
                     TSKVFEYLRTQRPILALVPAQNEAAGLLRSCGHDLICAMESADSIRNCLLRLFSQPRQ
                     DFRVPWELERKNQVDKLAEKLKTLF"
BASE COUNT         1225 a         1856 c         1666 g         1251 t
ORIGIN      
        1 ggggcgatgg cgtcgaaaaa gcagagctgg ccggagccca agatcttttg cagttccgcc
       61 gccagagcgt ccgacgtgag ggggccgctg cagaggatgc atacgctttc cggaagccgg
      121 gtcacctctt cgtgcaccat ttcgatcttg ggatgtgtcc gcaccagggt atccactttc
      181 tgggcaaaga gttcgcggtc cactgccagg gcgtgtccgg cgggaacgga gcagctttcc
      241 gcgatgggca gaagttttga acccagcagg cggagttctt ctttcagcat gcccgcgggc
      301 gtgtccggca gggtggattt gagggaattg ctgcaaacca gttccgcggc taggccggtt
      361 tggtgggctg gggtcatttt gcccgggcgc atctcgcaaa gcttaaccgc caatcctctg
      421 tccgcaagtt ggagagcggc ttcgctgccc gcgaggccag cgccgatgat ggtgatcgct
      481 ttcatgtttg ccttagcgga ttcccttcat cctcaggaag atgcccgcgc cgattagggc
      541 cggcaccacg tcgttgataa tgaagatggt cagtatcgtg ctggcgatgc tttcagcctg
      601 cagcccgatc ttggagagca gtgagatcgc gaagctttcc ttcagcccca gccccgctac
      661 cgttaccggt atgctgtagg aaagatggga aagcgacatc cgcttcacca cgtcccagaa
      721 cggcactgcc tggttgagat tcagcatcag ccagtattgg aaatggttaa tcaaggtggc
      781 tccgatcatc agcagggtga gccgcggcgc gtagcggatg tatcctgccc gcagcctggc
      841 ccagcgctgg ttcaggtaag taacaaaata cagccagaca ggcagggtca acaggccgat
      901 gaacagcagc caacgcgccc acgcaggaat tccgtgcgga aggtagagca gggctaggct
      961 ggcaaaaacg aagatgctcc aggttacgta tattcgttcc agcatgcagg aaatcataga
     1021 ggcggccttg ctgctgttcg aaacgtatgc caccttgccc atgatgcccc aactgcccgg
     1081 aacggcgaaa cgtagcgcct gtcctatcat gtaggattcg aacacctctt tccgcttcag
     1141 gacgaagagg ggattaatct gcagggagta ttgccaggcc agacactgcc accagtggcg
     1201 tagagctgcc aggccgagaa tggccagaat cagccagacg ggctggttga gcaggttttg
     1261 ccagaccttc ccaaaatcca tcctgcggaa cagcccatac aggataaggg ccgtcaccaa
     1321 cagcttgagc gcgaagctca gaacacgcca gacctttttc ctggtggtgg cgtccatgct
     1381 atttgcccag aagctcccgg gtgtgtttta gcgcggtttc gataagttcc ctcggtggtg
     1441 gattgatgct gccgtcgctc atcatgcggt ggcagagatc gcgggtagcc cggatgtccg
     1501 ctgcggccct ggcgaaaacc tcgtcccggg taagcggttg cgcggcaacg ccttccctca
     1561 cggcctgctc ggctacgtcg gcggcttcat tggcaaaaac gttttcctcg tccatgcggg
     1621 gcacgatatt gtcgggattt atctccctgg cttcggcaaa atcggcgagg gaatgggccg
     1681 cgcggatcgc catgccgtct gtgatctggc tggcgcgcac caacaaagcg cctttgagga
     1741 tgccgggaaa gccgcaggag ttgttgatct ggttgggaaa atcaccccgg ccggtggcca
     1801 cgatgaaggc tccggccgct ttggcgtcgt ggggatagat ttccggcacg ggattggcgc
     1861 aggcaaaaac tatcgccttg ggcgccatca gcctgatcca ttcaggcttg atgacatggg
     1921 gccccggtgt ggaaagcgat atcagaacgt ccgcgccctt catcgcttct tccatggtgg
     1981 ctaggcgttt ggggttggaa attctggcca gggcccactg ccttgatttg gcggggtttt
     2041 gggccaggtc gtcacggccg gcgtggagtc cgccccggct gtcgaacatc accaggtttt
     2101 gcgggtccag tcccgattgg atcaggatgc tggcgatggt gttgttggag gccccggcgc
     2161 cgaagagcac ggcgcggatg gactggatgc ttttcccggc caatttcagg gcgttcagca
     2221 acccggccag ggtcacgcag gcggttccct gggcgtcatc gtgccagacg gggatgccgc
     2281 agctggcgcg aagttcgtcc aaaacccggt agcagttggg ctgggagatg tcctcaagat
     2341 taaccgcgcc tacgctgggt tcaagcattt tcacaaactc gatgagccgc tccgggttcg
     2401 gtttgccagt ggcgtcccgg ttgttcacgc agagcgcgat agcgtcgaag ccgcccaggt
     2461 atttcatcag catggccttg ccttccatca cgcccaagcc tccggcgggg ccgcagtcgc
     2521 catcgcccaa aaccctggtg ctgtcgctca ccacagccac cagattgccc cggttgctta
     2581 gttcgtatga cctttcgtat ccgtcccgaa tcgaggtgga aacagcggaa accccgggcg
     2641 tataccacac gttgaaccaa ttgaagtccc agatgcctgc tttgggtagg gtttgcatct
     2701 tgccccggta gtgggcgtgg gtgagttccg agagtttttt caaaaaggcg gtctgcgccg
     2761 cggagcgttg ttcgggagcg tagttggctg aaaaatatgc ctggaggttg gccaggtcac
     2821 cgcgtaattt ttccatagtt catccagtat tttttgtgaa aatccaaaaa tgcgattgtg
     2881 cttggtttgt caacctttat cggtgatgtc cgggatgacg taaatgcggg atattcagct
     2941 gagaacgcca ccccgtcatc agcgttccgg cctccccctg tttcttcaat ttacgaaata
     3001 cagcttgaca ataaagcact tacaccaaaa gagtgtccgc gcccaaaggg tgataaaatg
     3061 aagatatgca tcgtgtccaa ttcccaccac accacggacg tgcgcctcta ttacaagatg
     3121 gcccgcagcc tggccaggtt gggcgaggtg catctgcttt gcgccagcgg ggttcgcaac
     3181 gccgccgtga atccctatca ggatgtggtg gaaaccgaat ccgcctggta tgccctgccg
     3241 ctgctctata agaaggcgaa aaagctcagg ccggacatcg tgatctgcgt ggagccgctc
     3301 acggtctgcg ttggcctggt tctgcgccgc aagctgcgct gccgggtggt cttcgatgtg
     3361 catgaattct tccccgacgc ctatgccgag cgtttccatc cgccttttag ctggatcgtg
     3421 aaggccctct acctggcttt cgagcgccag ctccaacgtc gggtggacgc caccatcgca
     3481 gtaaaccgga agattctgga ccagcttgtt ccccacggaa agggcaaaaa caccctcgtc
     3541 ctgcccaact atcccgtcaa aaacgtttgg gattacacct gcgagattcc gatggagctc
     3601 agcgccatct gcgagatgga ttttgacctc atctacatcg gcgggctcac ctccgaccgc
     3661 ggcatcttca agctgctcaa aagcgtctat ctcctccgca acgagtttcc cggcctgaac
     3721 gtcctcatcc tcggcaggtt cttttcgccc gaggtggaac gagaattcaa cgaacgtctc
     3781 aacgccctcg gcctcaacgc cgttatctat taccaaagct ggatcccggc ggaaaagatc
     3841 ggcctcctgc tcaagcgcag ccgggtgggc ctctggcttt tcaatcccca aaaccggcgc
     3901 ctgaggcgcg ccctgcccct caaggtgctg gaatactttg ccgccgggct gcccgtggtt
     3961 tccatcaaca cccctctgat gcgcggcttg atcgagtcaa accggcttgg cgcctgctgc
     4021 gaatatcaaa gcgacagcat tgccgaagcc atcgccggcc ttctccgcct cgggagggac
     4081 gaatacagtc aactcagcca gcgctgcgtc gaactcatcg accggaaata caactgggaa
     4141 gccgtcgaac cccggctcct ggacctaatg cggaagctcg gtcccagcta agatgcgcgt
     4201 tctttacatc agctatttct atccccccct gggcggcccc gccgccctgc gtaacctcaa
     4261 aacggtgaaa taccttgccg aggccggcat cgtttgcgac gtgatcaccg ttaaggacat
     4321 cgaatacctt taccgcgacc ccgccctcat ggaggaaaac gccgccgcca ccctcatccg
     4381 cgccgattcc ctggacccca tggccctgct gaaaaaaggc cggaacgccg gagggagcga
     4441 actttcccaa gtttacctga acaccccgga aaagctgaag ctgaaagtgc gccagctctg
     4501 gcctgtggac aacaagatcg gctggctgcc cgctttgata aaggccggac gcagggctat
     4561 ccgcgggcgg aaatacgacc ttatctacat ttccctgggc cccttttcct ccggcttcgg
     4621 cgccctcacc ctctcccgct ccagtggcat ccccctggcc gtggacctgc gcgactactg
     4681 gaccctcctg ggcgactacg atctccagag cggcatcttc aaaaaactct cccgcggcct
     4741 ggaaaagagc atctaccgcc actccagcct catcgttacg gcaactgaag gcatcggcac
     4801 cgatgccgca gaagccttcg gagcggaact ggcggaaaag acggtcaccg tctataacgg
     4861 ctgggacgaa gccgatttca gcgccctccc agagccgatt ccctcctccg gattccacat
     4921 cgcctatttc ggcaacatct acgcccgccg cagcttgcgc catttttacg ccgccctcag
     4981 gcgtctccgc gaagaaaacg ccctccccgc cggaaccagg gtgaagctct atggaaactt
     5041 cttccgcgaa accctccggg aagtggaaca gagcgggatc caggacctca ttgacatcat
     5101 cccccagctc agccaccgtg aagcccttgt cgccatgcgg aactcctccg ccctcattct
     5161 ggtcatcaac agctccagcc cccgcgggac cctcacctcc aaggtgttcg aatacctccg
     5221 cacccaaagg cccatcctgg ccttggtccc tgctcaaaac gaggcggccg ggctgctccg
     5281 ctcctgcggt cacgatctca tctgcgccat ggaatctgcc gacagcatcc gcaactgcct
     5341 cctgcgcctc ttctcccaac ccaggcagga tttccgtgtc ccctgggaac tggagcgcaa
     5401 aaaccaggtg gacaaactgg cggaaaaact aaaaaccctc ttctgaggat accgcttcac
     5461 aataatgtcc tctcggtcag gccctgagcg cctaaggtca tccctctccc acgcctcccg
     5521 caagataccc gcatcaaatg cgggcattat gagggaggca caactgggaa acaggaaagc
     5581 gccttaacgg ccagactaca tcgcttctac acggtcacac gctggcgtcg agcgaggcac
     5641 gggcagcgac gacagtaaat aagcctgtca ccccaaacct acgtcattcc gggcttgacc
     5701 cggaatccag tccatattcc gttatggatc ccggatcaat gtcacctcgc aggctcggta
     5761 acgtccggga tgacatagcc ctggaggctg gcgtcgagcg cagcatcgac gccagcaatt
     5821 aatgatttac ctcaatatat cctgtcgtcc atgctcacta cgctcgctgt acgcgaggcc
     5881 aactatttct ccctttttcc ttttccctcc atcactttcc cagtttgtgt aattcgtgga
     5941 caccccatcc tatttcagca gcaccagttt cttggtttcc ctgtaagcac cgcattgc
//
LOCUS       DAOOWR010000067         5976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13421_length_5976_cov_3.488600, whole genome shotgun sequence.
ACCESSION   DAOOWR010000067 DAOOWR010000000
VERSION     DAOOWR010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13421_length_5976_cov_3.488600"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1299
                     /locus_tag="PLB85_02650"
     CDS             178..1299
                     /locus_tag="PLB85_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72256.1"
                     /translation="MPVTLTEVKTRRDMKRFVYLPEVINAGRPGWVPPLYDDDKAVLN
                     PKKNPALSYCDTVYMLAWKDGKCVGRVAPIINHKYNDYAKVKTGRFGWFDAIDDLEVV
                     KALVDFAEKWLKERGMTRIVGPMGFTEEDPEGFIFMGYHENPTLACYQNTPTINEYME
                     KLGYGKEIDWFVYKLDMIKAMSPLYKKLFERASRSSLFRLKEFKSKKEINAYIRPVIR
                     LMNECFVDIYGYSPLSDEDVEHLAKRYMPLLEPRFIKVVETPEKEVIGFMVSIPNFSP
                     GIVKARGRLFPFGFIHILNAAKTTTQLDNYLGAVKAEYRGKGVDILMGYAQMKTAVDA
                     GFKIMDSHHEMEDNILMRAEMERSGGEIYKKFRLYSKDL"
     gene            complement(1677..3701)
                     /locus_tag="PLB85_02655"
     CDS             complement(1677..3701)
                     /locus_tag="PLB85_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU72257.1"
                     /translation="MNISRWLSIAIIVAVGMATCFADETAPTELKNQNRLNDADVLAE
                     WNGGVITRKDLDTKISHLPVNQQGRYRTVDGQIQVLDIMAMEEAFMAKAIQLGVDKDP
                     DVMKLIEAGTRQFYIQEYYKRNVSDLVIVTQADKQRYYDDNKQAFYEFPNITLNYIQT
                     KDEADALAAIAELQAGKSFGEVSDAYNINTYAKGIKGVIKNIRLNGNIPGVGNDLELE
                     KHIADSQPDPTAINGPFKTNSGWHLFQTVDYKAGRQKPFDEVMPELEQRARPGVETRI
                     LNDLTDRLKAKYAVAVDTTRVDEIDLKDRAKNEEIMDLNLISSSNPDLNITVAILLDR
                     YAQLSPQEQLFFSKGEAARQLLDQELVRSLLYADAKAQDYSQNLKENPEFEQMKRYYI
                     LNRAFRQLVVDAIQITSEEARAYYDEHIDDYSTPPHRKIEVLWFTDADEAEAARSKYD
                     LYVGFNDQTRIDKLIADKSVKPQLRVLQNIYDNGIITGVGPDKDFCDMVWDNPVGYIS
                     PVFTAASGDILFFRTLEETPTTAQSFTELEPRIYGMLKNQRQSTQQETVTQQLYEEFQ
                     MVKYPEKIRLELKAEDLFSMADNSARQRNFKDAISFYNQIITNFPNGADDYRASFMKA
                     FLIAEELKDEAQALQLFKDFLKKYPSGELNESAQFMIDSLEGNAVLEIEE"
     gene            3879..4322
                     /locus_tag="PLB85_02660"
     CDS             3879..4322
                     /locus_tag="PLB85_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOU72258.1"
                     /translation="MLRKLKNQKGFTLIEVLVVVIIVAILAALAVPIYMRHVEKSRSA
                     EAQTAIGAIRNTYKIYSQTYGSTQDYTIEKALKETNLDGSTSRYWKFEVTGNPPKKFI
                     ATSTEEFPGGPDKQVWFDVEEGKFHGYGVDQFTDPDTEGVEADTD"
     gene            4338..>4872
                     /locus_tag="PLB85_02665"
     CDS             4338..>4872
                     /locus_tag="PLB85_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOU72259.1"
                     /translation="MTIHELLRFTAEAGASDLHIAAGAHPMVRVNGRMKRLNLPILSA
                     EEVKTLVFGVMNEVQQEMFKENLEIDFSTKLSNDVRFRVNAFHQINGVSCAFRVIPNE
                     IKSYEELHLPDILKKLTHKEKGLILVTGPTGSGKSTTLATMIDTINDNRYCHIITVED
                     PIEFVHKSKNSLINQREL"
     assembly_gap    4873..4882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4883..5396
                     /locus_tag="PLB85_02670"
     CDS             <4883..5396
                     /locus_tag="PLB85_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOU72260.1"
                     /translation="KSFTAALRSALREDPDVILVGEMRDLETVSLALTAAETGHLVFA
                     TLHTGSCTKSIDRIIDMFPKEQQQQVRSMLSESLEAVLSQTLLPTKDGKGRVPALEIM
                     VANAAVRNLIREEKTYQIPSIIQASTKEGMQTRDQSLYNLVMNNLVERTIAEEFADNP
                     KQFASGAGFN"
     gene            5396..5851
                     /locus_tag="PLB85_02675"
     CDS             5396..5851
                     /locus_tag="PLB85_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72261.1"
                     /translation="MNEYNKMNKVKPLGLLSRQSGITILETMAVMTVVVVTVLAIYIG
                     VVYAERQLLTNYRDRVATLLITGELEMEYYRHTRSKPFELQLNKQYILDDTNPEHVLC
                     GNMTVTTRNAQETSNEQLLNYIQLEATFRWIDPGSKKERYIRMREDYFL"
BASE COUNT         1449 a         1518 c         1593 g         1406 t
ORIGIN      
        1 atcggacatg gttgccatag tgatttgttc cttaaagatt tatctcaaaa aagccaaaaa
       61 attcagtctc cactttttgg caaggatttt cttgcgcgga tttccgcttg acaggaaggg
      121 cgcttctccg tcttagtgca catgacactt aaaatactca cccacaaagg atagactatg
      181 ccagtaacgc tcacagaagt taagacccgc cgtgatatga agcgcttcgt ttacctgcct
      241 gaagtgatca acgccggccg cccaggatgg gtgccgcccc tttacgacga cgacaaagcc
      301 gtgctcaacc ccaagaaaaa ccctgcccta agctattgcg acaccgtgta tatgctggct
      361 tggaaggatg gcaaatgcgt gggccgcgtg gctcccatca tcaatcacaa atacaacgat
      421 tacgcaaaag tgaaaaccgg acggttcggc tggtttgatg ccatcgatga cctcgaagtg
      481 gtgaaggcac tggtggactt cgcggaaaaa tggctgaaag aacgcggaat gaccaggatt
      541 gtgggcccca tgggcttcac agaggaagat ccagagggct tcatattcat gggctatcac
      601 gaaaacccca ccctggcctg ctaccagaac acccccacca taaatgaata catggagaaa
      661 ctgggatacg gcaaggaaat tgactggttc gtttacaaac tggacatgat aaaggccatg
      721 agcccgctgt ataaaaagtt gttcgagcgc gccagccgca gcagtctctt ccgactgaag
      781 gaattcaaat ccaaaaaaga gatcaatgcc tatatccggc ctgtcatcag gctcatgaac
      841 gaatgttttg tggacatcta tggttattcc cccctcagcg atgaggatgt ggaacacctg
      901 gccaagcgtt atatgcccct gctcgaaccc cgtttcatca aggtggtgga aacccctgaa
      961 aaagaagtta ttggcttcat ggtctccatc cccaattttt cgcctgggat tgtgaaagcc
     1021 cgcggacgcc ttttcccctt cggctttata cacatcttga acgccgctaa aacaaccacc
     1081 caattggaca actatctcgg agccgtgaaa gccgaatacc gcggcaaggg cgtggacatc
     1141 ctgatgggct atgcccaaat gaaaaccgct gttgatgcag gcttcaagat catggacagc
     1201 caccatgaga tggaggataa tattctcatg cgcgccgaaa tggagcgctc aggcggggaa
     1261 atttacaaga aattccgcct ctattccaag gatctttaac gctcggtcct tagccccatc
     1321 caatacgccc cttgcctgcc tcccgcatga tactcgcatc aaatgcgggc attatgctgg
     1381 aggcttggca ttggcatatc acagacggct aagcaggggt taggtgaggg ctggcgtcga
     1441 gcgaggaacg agcttcgaca ccagcaaaga atgaacgctt cgacatatcc tatcctccac
     1501 tccatcagat tgttcctgtc gtccatgctc atttagttcg ctgtacgcta aggtggtgtt
     1561 aatatcggat acaggataag gaacaaccct tttccatcaa accagtagag gcatccgaca
     1621 tgaaaaaacc ccgccgaagc ggggtaatca actcataata aggtattggg ttacggttat
     1681 tcctcaattt ccaaaacggc attgccttcc agggaatcga tcatgaactg ggccgattcg
     1741 ttaagctcgc cgctggggta ttttttcagg aagtccttga agagctggag ggcttgggct
     1801 tcgtccttga gttcttctgc gatgaggaaa gccttcataa aggaggcgcg gtagtcgtct
     1861 gcgccgttgg ggaagttcgt gatgatttgg ttgtagaagg agatggcatc tttgaaattg
     1921 cgctggcggg cggagttgtc ggccatggag aaaagatctt cggctttcag ctcaagcctg
     1981 atcttttccg gatatttcac catctggaat tcctcgtaaa gctgctgggt aaccgtttcc
     2041 tgctgagtgc tctggcgctg gttcttgagc atcccgtaga tgcggggttc cagttccgtg
     2101 aagctctgag cggtggtcgg ggtttcctct agggtgcgga agaaaaggat gtcgccgctg
     2161 gcggctgtga agacggggct gatatagccg acggggttgt cccagaccat gtcgcagaaa
     2221 tccttgtcgg gtccgacgcc ggtgatgatg ccgttgtcat agatgttttg cagcaccctg
     2281 agctgaggtt tgactgattt atcggcgatc agcttgtcaa tgcgggtctg gtcattgaac
     2341 ccgacgtaga ggtcatattt gctgcgcgcg gcttccgctt catcggcatc ggtgaaccag
     2401 aggacctcta tcttgcggtg gggaggggtg ctgtaatcgt cgatgtgttc gtcgtaataa
     2461 gccctcgctt cctcgctggt gatctggatg gcgtccacga cgagctggcg gaaggccctg
     2521 ttcaggatgt aatagcgctt catctgttca aattctggat tttccttgag gttttggctg
     2581 tagtcttgag ccttggcatc agcgtaaagc aggctgcgga cgagttcctg gtccagcagt
     2641 tgcctggcgg cttcgccctt gctgaagaag agctgttcct gcggtgagag ctgggcatag
     2701 cggtcaagca gaatggccac ggtgatgttc aaatccggat ttgaggatga gatgaggttg
     2761 aggtccatta tctcttcgtt cttggcacgg tccttgaggt cgatttcgtc aactctggtg
     2821 gtatccaccg ccaccgcgta ttttgctttg agccggtcgg tcaggtcgtt gaggatcctg
     2881 gtttccacac ctgggcgggc gcgctgctca agctcgggca ttacttcatc aaaaggtttc
     2941 tggcgtccgg ctttgtaatc cacagtttgg aagagatgcc agccggagtt ggttttgaag
     3001 gggccgttta tcgcggtggg atcgggctgt gaatcggcga tgtgtttttc cagctccaag
     3061 tcgttgccca caccggggat gttgccgttg aggcggatgt ttttgataac tcccttgatt
     3121 cctttggcat aggtattgat gttgtacgcg tccgaaacct ccccgaagct tttccctgcc
     3181 tgaagttcgg cgattgcggc cagggcgtcg gcctcatctt tggtttggat gtagttgagg
     3241 gttatattgg gaaattcgta gaaagcctgt ttattgtcat cgtagtatct ctgtttatcg
     3301 gcttgggtga cgatcacgag atcgctgaca ttacgtttgt agtattcctg gatatagaac
     3361 tggcgggttc cggcttcgat gagtttcatg acatcggggt ccttgtccac cccaagctgg
     3421 atggctttgg ccataaaggc ctcttccatg gccataatgt ccagcacctg gatttgccca
     3481 tccaccgtac ggtagcggcc ctgttggttc accggcaggt gggagatttt ggtatccaga
     3541 tctttgcggg tgatgacacc tccgttccat tcggcaagga cgtcagcgtc attgagccgg
     3601 ttctggtttt taagttctgt gggagcggtt tcatccgcga aacaggttgc cattcccaca
     3661 gctacaataa tggcgattga tagccaacgt gatatattca tgtttgtctc cttaggtaat
     3721 ctgagagaat ttgaggcaaa tgcaaaaaaa agattggaca agaatgcgtc aagatattta
     3781 ttggcatctc tggattgatg aaattttcca gagaattggc acgcattgtg caagccataa
     3841 aagaacctga aaaaaagttc cgcaaggagg aaatacgaat gctcaggaaa ctgaaaaacc
     3901 agaagggttt taccctgatc gaagttcttg tggtggtgat tattgtcgcc atccttgcag
     3961 ccctggctgt tccgatctat atgcgtcacg tggaaaaatc ccgttccgct gaagctcaaa
     4021 ccgccatcgg cgccatccgc aacacttata agatttacag ccaaacctac ggcagcactc
     4081 aggactatac catcgagaaa gccctgaaag aaaccaacct cgacggaagc accagccggt
     4141 attggaaatt cgaagtcacc ggcaacccgc ccaagaaatt catcgccacc tccacggaag
     4201 aattccccgg cggacctgac aaacaggtgt ggttcgacgt tgaagaaggc aaattccacg
     4261 gttatggcgt tgaccagttc actgacccgg acacggaggg agtggaagcc gacaccgatt
     4321 aatctggagg aaacaagttg actatccacg aattgttacg cttcacggcg gaagcgggag
     4381 cttcggacct ccacatcgcg gctggcgcac accccatggt gcgtgttaac ggccggatga
     4441 aaaggctgaa tctgcccatc ctgtcagcgg aagaggtaaa gaccctggtt ttcggtgtga
     4501 tgaacgaagt ccagcaagag atgttcaagg aaaaccttga gattgacttt tccaccaaac
     4561 tgagcaacga cgtgcgcttc cgagtgaacg cattccatca gatcaacggg gtctcatgcg
     4621 ctttccgcgt gatcccaaac gaaatcaaaa gctatgagga actgcatctt cctgacattc
     4681 tgaaaaagct tacccacaag gaaaagggac tgatcctggt gaccggcccc accggcagcg
     4741 gcaaatccac caccctggcc acgatgatcg acacgatcaa cgacaaccgc tattgccaca
     4801 tcatcacggt ggaagaccca atcgaatttg tgcacaagag caagaacagc ctgatcaacc
     4861 agcgcgagct ggnnnnnnnn nncaagagtt tcacggcagc gctgagaagc gctctgcgcg
     4921 aggaccctga cgtgatcctg gtgggcgaaa tgcgagatct ggaaacggtg tccttggctt
     4981 tgacagccgc ggaaaccggc cacctggttt ttgccaccct gcacaccgga agctgcacca
     5041 agtcgatcga ccgcatcatc gacatgtttc ccaaggagca gcagcaacag gtgcgctcga
     5101 tgctctccga atcgctggaa gccgtgctct cacaaacttt gctgcccacc aaggacggca
     5161 aaggccgcgt tcccgccctg gagatcatgg tggccaacgc tgcggtgcgt aacctgatcc
     5221 gtgaggaaaa gacctaccag atcccctcca ttattcaggc cagcaccaag gaaggcatgc
     5281 agactcgcga ccagtctcta tacaacctgg tgatgaacaa ccttgtggaa aggaccattg
     5341 cggaagaatt cgctgacaat cccaagcagt tcgcctctgg cgcgggattt aactgatgaa
     5401 tgaatataat aagatgaata aggtgaagcc gcttggcctg ctaagcagac aaagcggcat
     5461 caccattttg gagaccatgg cggtgatgac agtggtggtc gtgactgtcc tcgccatata
     5521 tatcggcgtg gtttacgcgg agcggcagct tctcacaaac taccgcgacc gggttgccac
     5581 cctgctcata accggtgaac ttgagatgga atattaccgc catacccgca gcaaaccttt
     5641 cgagctgcag ttgaacaagc agtatatcct cgatgacaca aacccggaac acgttctgtg
     5701 tggaaacatg acggtcacga cgcgcaacgc ccaggagacg tccaacgagc agcttctgaa
     5761 ctacatacag ctggaagcta ccttcagatg gatcgatccc ggctcgaaga aagaacgcta
     5821 tatccggatg cgggaagact atttcctctg atgaatacca aacgaacctt cgcaaactcc
     5881 atcttgggcc gccaacgcgg cgtcaccctg gccgaactgg tcgttgtgat ggccatctct
     5941 gttattctta tatttgcggc agccttggga atcggc
//
LOCUS       DAOOWR010000068         5954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13490_length_5954_cov_4.426513, whole genome shotgun sequence.
ACCESSION   DAOOWR010000068 DAOOWR010000000
VERSION     DAOOWR010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13490_length_5954_cov_4.426513"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="trmFO"
                     /locus_tag="PLB85_02680"
     CDS             <1..813
                     /gene="trmFO"
                     /locus_tag="PLB85_02680"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOU72262.1"
                     /translation="ADSLDFSRIYRKDRYDKGDPDYLNCPFTREEYYTFIEALLSGEQ
                     YQAREFENEFFRDLKFNYYENCTPVEELARRGKDTLRHGVMRPMGLEHEGKIPFAVLQ
                     LRTENRDQTAFNLVGCQTMLRQAEQKRIFRLIPGLEQAEFLRYGSIHRNSYLNAPQAL
                     APNLTLRNARHVFVAGQLSGVEGYMECVATGLLVTRILAEGIPMLPPETILGQLWRRL
                     IDPSQKNFQPVNANFGLLPALEVPVRDKKLKKLSLSQRGLEALKTFLEKESD"
     gene            826..2154
                     /gene="hflX"
                     /locus_tag="PLB85_02685"
     CDS             826..2154
                     /gene="hflX"
                     /locus_tag="PLB85_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU72263.1"
                     /translation="MNDREQERLVEEFDLEPDTWDDFERAEKTAFLAAIVRPGESDAE
                     AAASLEELERLADTAGIEVLGTYTQRRGHPERASFFGKGFLEEISQKMRQAQAEVLIV
                     NEELTPIQGRNIENNFGIRVIDRTEVILSIFHDRARTREAKLQVRLAELQYQLPRLRR
                     LWGHFDKERGSQRASGGAATRGMGEKQIEIDKRLIRQQIRKINKAIADITNQKETQRK
                     QRDRTKKICLVGYTNAGKSTLFNSITEAGVLVEDKLFATLDSTSRQLKLSTSFPVVIS
                     DTVGFISNLPHHLVASFSATLMEVRDADLLLHVVDISDERFGYYIEQVNSVLASIGAQ
                     DIPQLLIMNKTDLVDDTICLFVQRRYPEAVLISALNIEQVDKLLPKLEQKLFQNRLCK
                     VFLPFDKGSLASLLYRIANIQKEEYQADGIYLELEISEDDLHHVQDYLLT"
     gene            2210..2875
                     /locus_tag="PLB85_02690"
     CDS             2210..2875
                     /locus_tag="PLB85_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOU72264.1"
                     /translation="MFKQITATEAAAMITPHDRVAIGGFLAVGAPESIIDALVAAGTK
                     DLHIIVIASDWENRGIGKLVVNHQIKSAQVSHTGTNKEIQNQMNSGEISVELVPQGTL
                     MERCRAFGMGLGGILTPTGIGTVVEEGKQIINLDGEDYILETAIPSDFALIRAWKADR
                     RGNLIYSKTARNSNPIMAMMGKITIAEVDEIVETGELDPEHIVTPGVFVNYICLSKEA
                     NNG"
     gene            2868..3509
                     /locus_tag="PLB85_02695"
     CDS             2868..3509
                     /locus_tag="PLB85_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOU72265.1"
                     /translation="MDKRAMIGARIAKELKDGDYVNLGIGLPTEAANHIPPGVHVIFQ
                     SENGVMGVGPAPAEGSEDQDMINAGGGFITTVPGASYFDSATSFGIIRGGHLDITVLG
                     ALQVDQHGNLANWMIPGKIIPGMGGAMDLVTGAKKVIVAMEHCDKRGNPKILKECTLP
                     LTAKGKVSLIISDLAVIEVTPHGLLLQEVSEGYTVEDVIKATEADLTIPGHLR"
     gene            3536..4105
                     /locus_tag="PLB85_02700"
                     /pseudo
     CDS             3536..4105
                     /locus_tag="PLB85_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
     gene            4149..4679
                     /locus_tag="PLB85_02705"
     CDS             4149..4679
                     /locus_tag="PLB85_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOU72266.1"
                     /translation="MDEKPKIGISSCLLGNKVRYDGRHKYDSWLVETLGKYVTYVPVC
                     PEVECGLSVPRDPMRLVGEVESPRLLTIKDRTDHTPRMLEFCVRKLSELADAELCGFI
                     FKSKSPSCGTRRVIVHPDKNGSGGKEGMGIFAREFIKAFPLLPVEEESRLQDPALLEN
                     LIERVYIIQRRNKPRR"
     gene            5055..>5954
                     /locus_tag="PLB85_02710"
     CDS             5055..>5954
                     /locus_tag="PLB85_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOU72267.1"
                     /translation="MKTVKSLVLCCVLLCGVAVLGARGPHWLWARGAGGTGNDEGFSI
                     AIDSQGNQYVTGTFESTVSFGSHILTSNGNYDIFVAKLDPDGNWLWVVQAGGTGPDWG
                     GGVAVDGAGNAYVTGSYRGTVSFGSYTLTAYVNYNDMFVAKLNTNGTWLWAVSAGGGF
                     YDQGNKIAVDGWGNAYMAVQSDSETITFGSCTLTRFGPGTDIYVAKLDPYGNWLWAVQ
                     AGGTADDIGNDIAVDGEGNPWVTGKFRGTATFGSQTLTVSGHNDVFVAKLNTNGNWLW
                     AVQAGGTADDIGHGIALDGSGNAW"
BASE COUNT         1446 a         1679 c         1623 g         1206 t
ORIGIN      
        1 gccgacagcc tcgatttcag ccggatttac cgcaaagacc gctatgacaa aggtgacccg
       61 gattacctca actgcccttt cacccgcgag gaatactaca ctttcattga agccttgctg
      121 agcggggagc agtatcaggc gcgcgaattt gagaatgagt ttttccggga cctgaagttc
      181 aactattatg aaaactgcac cccggtggag gaacttgccc gcaggggaaa agacactctg
      241 cgacatgggg ttatgcgtcc gatgggctta gaacacgagg gtaaaatacc cttcgcggtt
      301 ctccaactaa gaaccgagaa tagggaccaa acagctttca acctggttgg ctgccagacc
      361 atgctccgcc aggccgagca gaaacgtatt ttccgcctca tccccggctt ggagcaggcg
      421 gaattcctgc gctacggctc catccaccgc aacagctatc tgaacgcgcc ccaagccctg
      481 gctcccaacc tgactctgcg caacgcccgc catgtctttg tggccggcca gcttagcggc
      541 gtggagggct acatggaatg cgtagccaca ggtttgctgg tgaccaggat cctggctgaa
      601 ggcatcccga tgctgccgcc ggaaacgata ttgggccagc tctggcggcg cctcatcgat
      661 cccagccaga agaacttcca gccggtgaac gccaattttg gcctgctccc agctttggaa
      721 gtgcccgtca gagacaagaa actaaagaaa ctgagcctct cccaacgcgg gctggaggcc
      781 ctgaaaacct ttctggaaaa ggaaagtgac tgagcaggga ggaccatgaa cgacagagag
      841 caagaacggc tggtcgaaga atttgacctc gaaccggata cctgggatga tttcgaacgg
      901 gcggagaaaa cggcgttttt ggcagctatc gtgcgccccg gcgaatccga tgccgaggct
      961 gctgccagcc tggaggaact ggagcgcctg gccgacacag ccgggattga ggtcctgggc
     1021 acttacaccc aaagacgcgg ccaccccgag cgggccagct ttttcggcaa gggcttcctg
     1081 gaggagatca gccagaaaat gcgccaggcc caggctgaag tgctcatagt gaatgaagag
     1141 ctgactccca tccagggccg caatatcgag aacaatttcg gcatcagggt gattgaccgc
     1201 accgaggtga tcctttccat cttccacgat cgcgcccgca cccgcgaagc caagctgcag
     1261 gtgcgtttgg cggaactcca ataccaactt ccgcgcctgc gccggctctg gggccatttc
     1321 gacaaagaac gcggctccca gcgcgcttcc ggcggtgcgg ccacgcgcgg catgggcgaa
     1381 aaacagatcg agatcgacaa acgcctcatc cgccagcaga ttcgcaagat caataaagcc
     1441 attgccgaca tcacaaacca gaaggaaacc cagcgcaaac agcgcgaccg caccaagaaa
     1501 atttgcctgg taggctacac caacgccggc aaatccaccc tgttcaattc catcacggaa
     1561 gccggggtac tggtcgagga caagcttttc gccactctcg attccacctc gcgccagctc
     1621 aagctatcca cctccttccc tgtggtgatc tctgacacgg tgggcttcat ctcgaacctg
     1681 ccccaccact tggtggcctc gttcagcgcg actttgatgg aggtgaggga cgccgatttg
     1741 ctgctgcacg tggtggacat ctccgacgag cgcttcggct attacatcga gcaggtgaac
     1801 tccgtgctgg ccagcatcgg ggcgcaggac atcccccaac tgctgatcat gaacaaaacc
     1861 gacctcgtgg acgacaccat ctgccttttc gtgcagaggc gctatcccga agccgtgctg
     1921 atcagcgcgc tgaatattga gcaggtggac aaactgcttc caaaactgga gcaaaaactt
     1981 ttccaaaacc gcctctgcaa ggtctttctg ccttttgaca agggttccct ggcctcgctt
     2041 ttgtacagga tcgcgaacat tcaaaaggag gagtaccagg ccgacggcat ctatctggaa
     2101 ctggagatca gtgaggacga cctccaccat gtgcaagact atctgttgac ataaaacccc
     2161 gcgtggaaaa taatgtccgc ataatatcat taaagtcata ggagaagtaa tgttcaaaca
     2221 aatcactgcg acagaagccg cggccatgat caccccccat gatcgggtcg ccatcggcgg
     2281 atttctggca gtaggcgcgc cggaaagcat catcgacgcc ctcgtcgccg ccggcaccaa
     2341 agacctccat atcatcgtca tcgcctcgga ctgggaaaac cgcggcatcg gcaaactggt
     2401 ggtgaatcac cagatcaaga gcgcccaggt ttcccacacc ggcaccaaca aagagatcca
     2461 gaaccagatg aattccggcg aaatctctgt ggaactcgtc ccccagggca cgctgatgga
     2521 gcgctgccgc gcttttggca tgggtttggg cggaattctt acccccaccg gaatcggcac
     2581 cgtggttgag gaaggcaagc aaatcatcaa tttggacgga gaggattaca tcctcgaaac
     2641 cgccatcccc agcgactttg cccttatccg cgcctggaaa gccgaccgca ggggaaacct
     2701 catctacagc aaaactgccc gtaacagcaa ccccatcatg gccatgatgg gcaagatcac
     2761 cattgccgaa gtggacgaaa tcgtggaaac aggcgaactg gatcccgagc atattgtcac
     2821 tcccggagtt tttgtgaatt acatctgcct gagcaaggag gccaacaatg gataaacgcg
     2881 caatgatcgg agcacgaatc gcaaaagaac tcaaagacgg cgactacgtt aacctgggca
     2941 tcggcctgcc cacagaggcc gcgaaccata tcccgcctgg cgttcacgtc atcttccaat
     3001 cagaaaatgg cgtcatgggc gtgggccccg ctcctgctga aggcagcgag gaccaggaca
     3061 tgatcaacgc cggtggaggc tttattacaa ctgttcccgg agcttcttac ttcgattccg
     3121 ccactagttt cggcatcatc cgcggcggcc atctggacat caccgtcctg ggcgccctgc
     3181 aggttgatca gcacggcaat ctggccaact ggatgattcc cggcaagatc atccccggca
     3241 tgggcggcgc gatggacctt gtgaccgggg ccaagaaagt gattgtcgcc atggaacact
     3301 gcgacaaacg cggcaacccc aaaatcctca aggaatgcac cctgcccctc accgccaaag
     3361 gcaaggtgag cctaatcatt tctgacctcg ccgtaatcga ggtgaccccc cacggcctgc
     3421 ttctgcaaga agttagcgaa ggctacacag tggaagacgt gatcaaggcc accgaggcag
     3481 atctgaccat tccgggacat cttagataga gagccatccc ccattagcaa agcgtgtgta
     3541 tcagttcaaa atcacccttc tggacaccaa actgcccatc tggagacgca tccaggttgc
     3601 ggaaaactat cgcttctggg acctccacat ggcaattcag aatgccatgg gcaggcagaa
     3661 ctgccatctt cactaattca cgatttattc gcccaaggaa atgggacttg ttgagatcgg
     3721 attgggggag gaggatttta acaagattcg cattttcgag cgtatttcca agtatttttc
     3781 cctggaaaac accgacgcgt tgtattggta cgatttcggc gacgattgga gccataaggt
     3841 cactttggaa aagatcatcc ctgccgaccc caagcacact tatccacgct gtatagccgg
     3901 gaaaagggcc tgcccgcctg aagattcagg tggcgtatgg ggcttttacg aaatgctgaa
     3961 tgttctggaa gaccccgaac acgaagaaca cgaagacctg cttgaatggc ttggcgatgc
     4021 ttatgacccg gaacatttcg atcccaaaga ggttctgttt gaggacccca aggaaagcga
     4081 gaaagccata acctcattgt attgatgaat gaagatagcc caagccggga gattggggac
     4141 gacctgccat ggatgaaaaa ccgaaaatcg gcatcagttc ctgccttctg ggcaacaagg
     4201 ttcgctacga cggccggcac aaatacgatt cctggctggt ggagaccctt ggcaaatatg
     4261 taacttatgt gcctgtctgc cctgaggtgg aatgtggcct ttccgtcccc cgcgacccca
     4321 tgcgcctcgt gggggaggtg gaaagtcccc gtctgcttac catcaaggac cggacagacc
     4381 acaccccacg catgctggaa ttctgcgtcc gcaagctttc cgagctcgcg gacgcggaac
     4441 tctgcggctt catattcaag agcaaatcac cttcctgcgg cacgagacgg gttatagtcc
     4501 atccagacaa aaatgggtct ggcgggaaag aaggcatggg catcttcgcc cgggaattca
     4561 ttaaagcctt tcccctcctg cccgtggagg aggaaagccg gctgcaggac cccgcgctgc
     4621 tggaaaactt aatcgagcgc gtctacatca tacagcgtcg gaacaagccg cgccgctaac
     4681 ttttttccta cttttccctt tctgattata cggattcgat tgattcacga atccttttcc
     4741 tctctcaaac ccgctattgg tcaactaggc gtcgaccttc cactcagcct ggagtccagc
     4801 gagcctgcga gcttggacta caggacactt gtgagctttt aattatttgc tgccgtcgat
     4861 gctcgtccct cgctcgacgc cagcttcccc gtcaacctcg ctccaaagga gcaaagggca
     4921 gggatggacg cctgtccgtt ttgcctgtca tcagcacccg aacttgattc atcttgtggt
     4981 gattattttt cttgacacat tttacaacac cacgataatg tggcccagtt aaaaccagtc
     5041 ttatggaggt aaaaatgaag acggttaaaa gcttggtttt gtgttgtgtg ctgctctgcg
     5101 gagtggcggt tttgggtgcg cgggggcccc actggctgtg ggcgcgtggc gcgggaggaa
     5161 ctggcaatga tgaaggcttt tccatagcga ttgacagcca gggcaaccag tatgtgaccg
     5221 ggacgtttga aagcaccgtc agttttggca gccatatcct gacctcaaac ggaaactatg
     5281 atatttttgt cgccaaactg gaccccgacg gcaactggct ctgggtggtc caggcgggag
     5341 ggacaggacc tgattgggga ggtggcgtcg ccgtggatgg cgcgggcaat gcctatgtaa
     5401 ccgggtctta tagaggcaca gtcagtttcg gcagttatac cctgacggcg tatgtcaatt
     5461 acaatgatat gtttgtcgcc aaactgaata ctaacggaac ctggctctgg gctgtcagcg
     5521 cgggaggggg attctatgat cagggaaata aaatcgccgt ggatggctgg ggtaacgcct
     5581 atatggccgt gcaatctgac agcgaaacaa ttactttcgg cagttgtacc ctaaccagat
     5641 ttggacctgg aacggatatc tatgttgcca agctggaccc atacggcaac tggctctggg
     5701 cggtccaggc aggaggaaca gcggatgaca ttggaaatga catcgccgtg gatggcgagg
     5761 gtaacccctg ggtgaccggg aagtttagag gcaccgccac tttcggcagc cagaccctga
     5821 cggtaagcgg acacaatgat gtgtttgtcg ccaagctgaa cacgaacggc aactggctct
     5881 gggcggtcca ggcaggagga acagcggatg acattggaca tggcattgcc ttggatggct
     5941 cgggcaacgc ctgg
//
LOCUS       DAOOWR010000069         5920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13599_length_5920_cov_3.696164, whole genome shotgun sequence.
ACCESSION   DAOOWR010000069 DAOOWR010000000
VERSION     DAOOWR010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_5920_cov_3.696164"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLB85_02715"
     CDS             complement(<1..396)
                     /locus_tag="PLB85_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU72268.1"
                     /translation="MMNIEEINQKVEQSSAVLDTLRAEIAKVIVGQETVIDRLLIGLL
                     ANGHVLIEGVPGLAKTLILSTLAGAFDASYKRIQFTPDLLPADITGTLVYNQKTGEFT
                     PKQGPVFANFILADEINRAPSKVQSAMLEA"
     gene            complement(500..691)
                     /gene="rpmB"
                     /locus_tag="PLB85_02720"
     CDS             complement(500..691)
                     /gene="rpmB"
                     /locus_tag="PLB85_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU72269.1"
                     /translation="MSRICDICGKQAQVGNHRSHALNATKRRFYPNLHEVRAIIGSGI
                     KKVKVCSSCLKANKVRKAV"
     gene            complement(889..2019)
                     /locus_tag="PLB85_02725"
     CDS             complement(889..2019)
                     /locus_tag="PLB85_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72270.1"
                     /translation="MSANLDINQRIGLFDSLEQDVVENYTQELTRLKLKKGEILTLDY
                     KENQSLFLIEKGSLEISCKKKKGEKGELTLIVGPGEFCGEFALLSNAPADATATALEA
                     SELLEIPYDKFNKLSFSQPAVLINVIRALSAKLRDTNLKFSDLMDDMIQRNRLTAIGM
                     TASKIIHDIKTPLTVISLTAQLMESLYPDSSEFTQSIVQQTKLVDQLVREVLDYVEGT
                     PHAIIPRKVDMNSFLMDLKETYGASLRGRNISLNIDNQCFDPVYFDEERIRRVITNLL
                     RNSSEAMNEDGEISIVTSLAAKWLQISVIDNGPGIPESVVPQLFKPFFAYNKPNGTGL
                     GLPICQKMVQEHNGRVDYVPVEPHGSRFDIRLPQNIPLEIAR"
     gene            complement(2490..3197)
                     /locus_tag="PLB85_02730"
     CDS             complement(2490..3197)
                     /locus_tag="PLB85_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72271.1"
                     /translation="MNIKPSLILTALLLLTLSLSAQDFLAKTAVGLHAGTQSGTGYSM
                     RFMGEKHGLQTTFGAGTWGDDDVYFPTRFTYWYNDYYTDYWYRDIESGSEDAPYDTLI
                     TITQNGRYNVVNLGLNYIYTLDKFDVLMQKDRGRLYVMAGGSYKYYRQNTFTKDYRWV
                     TVEDTLDWDHYEPVDNSEPISKSKLEHRWTAGAGLGVELGLGKNFRVALELPITYNWE
                     QRIVMYIPQIGFYYYFK"
     gene            complement(3490..4281)
                     /locus_tag="PLB85_02735"
     CDS             complement(3490..4281)
                     /locus_tag="PLB85_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU72272.1"
                     /translation="MKFRLPPDPVNNTDGMAVHWSRHIVLILDFLLPALILAAGTWLI
                     RSRGLDLRFQDAFYTGSGNWIGNHIKWFDFIYKYGTVPAVIVSITGMVVFVVGFFSRN
                     LARWRRSGLFLALVMLLGPSLLVNAVFKQYWGRPRPNQLALYGGEFAYEAPLTIDHAS
                     TGEAFPSGHASMGFYFFALYFIFRGRKPRLAAWAFIITLLYGFGMGLVRMTQGGHFFS
                     DVLWAGGIVWFSCALLYYLLKIDRLVFLKPLAPPEEDIEKQKDSA"
     gene            complement(4308..>5920)
                     /locus_tag="PLB85_02740"
     CDS             complement(4308..>5920)
                     /locus_tag="PLB85_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU72273.1"
                     /translation="IKREELLSRIQVDIELDGEVKNITQYGAFIDLGGIDGLLHLTDM
                     SWGKINHPTDMLNIGDKVKVKVIKFDPETNKISLGMKQLVPHPWENVAIKYPEGVRVT
                     GKVVSVKSFGAFVELEPGVEGLVHISEMSWTKKITDARKIVKVGDTVNAIVLELDKEN
                     RRISLGMKQMEANPWLSIEDHYPVGSVITRPVKSLTAFGAFVEVADGIDGLIHISDIS
                     WTKRIYHPREVYHKGQEVEAVILSIDRALHRIALGVKQLHPDPWEHLNESLPINTEVI
                     GKIGKLIPKGVLVDIKVGEDIVEGFIPISHLAIPKLEHSEEAFHVGEELPLKVIELDM
                     ENRRLILSVKAFFFSRDPKLQEEYIAIHEQYMRERIARLQKKRADRLAREKQKQEAAA
                     KLAEEAKSQVEATAAEDGSDAVETLTEEVPAEEPAADEPVTEEAVEKEEVSAEEPVPT
                     EPVTEEAVETQEVPAEEQVPEEPVSEETVETEEAPTEEQVPEEPVAEEAVETVETPTE
                     EPVTEEPAAEEITETPEETPAEPETQDN"
BASE COUNT         1248 a         1438 c         1678 g         1556 t
ORIGIN      
        1 cgcctccagc atcgcggact gcaccttgga gggggcgcgg tttatctcgt ccgccagaat
       61 gaaattggca aagacagggc cctgtttggg tgtgaattcc ccggttttct ggttatagac
      121 caaagtgccc gtgatatcgg ctggaaggag gtccggggtg aattggatgc gtttgtagct
      181 ggcgtcgaaa gcgcctgcga gagtgctgag gatgagggtt ttggccaggc cgggaactcc
      241 ttcgatcagc acgtggccgt tggccaaaag cccaatcaac aggcgatcta tcaccgtttc
      301 ctgacccact atcaccttgg cgatctcggc gcgaagggtg tcaagaaccg ctgagctctg
      361 ctccaccttc tgatttatct cttcaatgtt catcatttct ccaatatatc ccccctaaaa
      421 aaaacgggaa caggcagtta ccgtattccc gatcagattc aattgtctat cggctccgcg
      481 aagacattgg ctgtggctgt taaaccgcct tgcggacctt gttcgccttc aggcaggaag
      541 agcagacttt cactttctta attccgcttc cgatgatggc gcgaacttca tgcaaattcg
      601 gataaaaacg cctcttggtg gcattcagcg cgtggctgcg gtgattgccg acctgggctt
      661 gctttccaca tatatcgcat attcttgaca tttttcaccg tccagattga tttcttgaaa
      721 gccatctttt tcgcctccgc atttttgaca aggtttattt acattggctg accttattcc
      781 cggccaccaa tcaggatcgg tcacgaaaag caaatgatgg gttgtagcgc ggtgagcttt
      841 agtcagcctg tctaatctgg ctgggttatt ttggcagtgg ttcttggctc agcgggctat
      901 ttcgaggggg atgttttggg gcaggcggat gtcgaaacgg gagccgtgag gttccacagg
      961 cacgtaatcc actcgcccgt tatgttcttg caccattttt tggcagatgg gcaacccaag
     1021 acctgtgccg ttgggtttgt tgtaggcgaa gaagggctta aagagttggg ggaccacgct
     1081 ttctggtatg ccggggccgt tgtcgatgac gcttatctgc agccattttg cggcgaggga
     1141 agtgacaatg ctgatctcac cgtcttcatt catggcctcg gaactgttgc gaagcagatt
     1201 tgtgatcacg cgtcggatgc gttcttcgtc aaaatagacg ggatcaaagc attggttgtc
     1261 gatatttagg gatatgttgc gtccgcgcag ggaggctccg taagtctctt taaggtccat
     1321 gaggaaacta ttcatatcca ctttgcgggg gatgatcgcg tggggggtgc cttccacgta
     1381 atccagcact tcacgcacca gttggtccac cagttttgtt tgctgcacaa tgctttgggt
     1441 gaattccgag gagtccggat agagggattc catcagttgt gcggtaagcg aaatcacggt
     1501 gaggggggtc ttgatatcgt gaatgatctt gctggcggtc atgccgatgg cggtcaggcg
     1561 gttgcgttgg atcatatcgt ccatgaggtc tgaaaacttg agattggtat ctcgaagctt
     1621 ggcagagagg gcgcgtatca cgttgataag cacggcgggt tgggaaaagc ttagcttgtt
     1681 gaatttgtcg tagggtattt ccagcaattc ggaggcttcg agcgcggtgg ctgtggcatc
     1741 tgccggagca ttgctcagca gggcgaattc accgcaaaat tcacccggtc ccacgatcag
     1801 ggtgagttct cctttttcgc cttttttctt tttacaggag atttcaaggc tccctttttc
     1861 tatcaggaaa agggactgat tctccttata atcgagggta aggatttccc cttttttaag
     1921 tttgaggcgg gtaagttcct gggtgtaatt ttccacaacg tcttgttcca agctgtcaaa
     1981 caaacctatc cgctgattta tgtccagatt cgcgctcatc gctcctcccg gttttttaaa
     2041 ttatcaaccc ctatatttta tctcaggatt tgtcgtcaat ctttttttgc cgcccagcgt
     2101 ggagaaaagc caagttttaa cccaaatatg gagccgcggg gagagaatcc tgtgtttttt
     2161 tggggtttgg cagtggtttc gtcctttgcc gcatccgatt tgcatccgct tcgcctcccc
     2221 ttaacttccc gtttgagctg cgggaactca acgggaagtg aatgggttgt gaaagagaaa
     2281 gggcaaatgg ggagcagatc agggttaaac aaagctcgta aggggttatg gcgaaaaggg
     2341 ctcaggtgta tcgggggcag tcattcccac aatatttgaa cctcagccgg aaacaagcaa
     2401 aagcaaggta aacaggcagt tccggtttac acggattcac ggatcggact gatttgctga
     2461 tttatgttag tgccgaagcc gcctgctgcc tatttgaagt aatagtagaa cccgatctgg
     2521 gggatgtaca tcacgatgcg ctgttcccag ttgtaggtga tgggcagttc cagggccacg
     2581 cggaagttct tgcccaaacc aagttccacg cccaatccgg cgccggctgt ccatctgtgc
     2641 tccagcttgg atttgctgat aggttcgcta ttgtccacgg gttcgtagtg gtcccaatcc
     2701 agagtatcct caaccgtaac ccagcggtag tcttttgtga aagtattctg ccggtaatac
     2761 ttgtaggagc cgccggccat cacataaagg cggccgcggt ccttttgcat caaaacgtcg
     2821 aatttgtcga gggtatagat gtagttgagg ccgagattga ccacgttgta gcgcccgttt
     2881 tgggtgatgg ttatcagagt gtcgtaggga gcgtcttcag acccggactc gatgtctcta
     2941 taccaataat cggtgtaata gtcattatac cagtaagtaa agcgagtggg gaaatagacg
     3001 tcgtcatcgc cccaggtacc ggctccaaaa gtggtttgca ggccgtgttt ttcgcccatg
     3061 aagcgcatgg aatagcctgt gccgctttgg gttccggcgt ggaggccaac ggcggttttg
     3121 gcaaggaaat cctgggctga aagcgaaaga gttagcagca gcaaagctgt caggatcagg
     3181 ctgggtttta tattcacggt tcacctctat ggttgatttg agccattggt tccgcctatg
     3241 gcataagtgt caagaaattc cgagcaagac tcattcgcga aatggcttgg ccatgaatta
     3301 cacgaattgg gggaagagcg gttcttttgt gttctctacg gatgcaattt attcaactga
     3361 tgatacggat ttacttatcc aagagaatga tagacacacg caacataaac ctgtcgcgcc
     3421 tctttttcgc agccgtttcc tcttgcttgg gcggcgaaca tggccagaaa cagggccaaa
     3481 tccaatttgc tatgcagaat ccttttgttt ttctatgtct tcctctggcg gggctaacgg
     3541 cttgagaaag acgagacggt cgatcttcag aagataataa agcagcgcgc aactgaacca
     3601 gacaatgcct ccggcccaga gcacgtctga gaaaaagtgg ccgccctggg tcatgcgcac
     3661 aaggcccatg ccaaaaccgt agagcagtgt gatgatgaag gcccaggcag cgagacgggg
     3721 tttgcgaccg cggaagatga agtagagggc aaaaaagtaa aaacccatgg aggcatggcc
     3781 gctgggaaaa gcttcgccgg tactggcgtg atcaatggtt agaggggctt cgtaggcaaa
     3841 ctccccgccg tagagggcca gttggttggg acgggggcgt ccccagtatt gtttgaagac
     3901 agcgttcacc aaaaggctgg gaccgagcag catcaccagg gccagaaaca ggccggagcg
     3961 gcgccaacgg gccaggtttc gggagaaaaa acccaccaca aagaccacca tgccagtgat
     4021 ggaaacgatg acggcgggca cggtgccgta tttgtagatg aagtcaaacc acttgatatg
     4081 gttgccgatc cagttgccgc tgccggtgta gaaagcgtcc tggaagcgca gatcaaggcc
     4141 acgggagcgg atcagccagg tgcctgctgc caggatcagc gcgggcaaca ggaaatccag
     4201 tatcaaaacg atatggcggc tccagtgaac cgccataccg tctgtattgt taaccgggtc
     4261 tgggggcagt ctgaacttca aggttcggtg ctccaggctc cggttgttca gttgtcttgg
     4321 gtttccggtt cggccggggt ttcctcaggg gtctcagtga tctcttcggc cgcaggttct
     4381 tctgtgacag gctcttctgt tggggtttct actgtttcca cagcctcttc agccacgggt
     4441 tcttccggga cttgctcttc tgtgggagct tcttctgttt ccacagtttc ttcagacaca
     4501 ggttcttccg ggacttgttc ttctgctgga acttcttggg tttcaacagc ttcttcagtc
     4561 acgggttctg tcgggactgg ctcttcagca gagacttctt ctttttccac agcctcttca
     4621 gtcacaggtt cgtctgcggc tggttcttct gccgggactt cctcggtcag ggtctccacg
     4681 gcgtcagagc cgtcttcagc agcagttgct tcaacctgag atttggcttc ttcggcgagc
     4741 ttggcggctg cctcttgctt ctgtttttca cgggccaggc gatcggcgcg tttcttttgc
     4801 agacgggcaa tgcgctcgcg catatattgc tcgtggatgg cgatgtattc ttcctggagt
     4861 ttgggatcgc gggagaagaa gaaagccttc acgctgagga tgaggcggcg gttttccata
     4921 tccagttcga tcaccttgag aggtagttcc tcgccgacat ggaaggcttc ctcagagtgt
     4981 tccagcttgg gaatggcgag gtgggaaatg ggtatgaacc cttccacaat gtcttcgccg
     5041 accttgatgt ccaccagcac gcctttggga atgagtttgc cgatcttgcc tatcacttcg
     5101 gtgttgatgg gcagggattc gttcagatgc tcccagggat cgggatgcaa ctgtttcacg
     5161 cccagggcga tgcggtgcaa ggcacggtcg attgaaagga tcacggcctc cacttcctgg
     5221 cctttgtggt aaacctcgcg aggatggtag atgcgcttgg tccagctgat gtcggatatg
     5281 tggatcagac catcgatgcc gtccgcaact tcaacaaaag caccaaaggc ggtgaggctt
     5341 ttcacgggac gggtgataac actgccgacg gggtaatggt cctcgatcga gagccaggga
     5401 ttggcttcca tctgcttcat gcccagggag atgcggcggt tttccttgtc cagttccagc
     5461 acgatggcgt ttacggtgtc gcccaccttc actatcttgc gggcatcggt tatttttttg
     5521 gtccagctca tttcagagat gtgcactaag ccttccacgc cgggttcaag ctccacaaaa
     5581 gctccgaagg atttcacgct gaccactttg ccggtgacgc gcacaccctc ggggtatttg
     5641 atggccacgt tttcccaagg atgcggcacc agttgtttca tgccgaggga gattttattg
     5701 gtttcgggat cgaatttgat cactttcact ttcaccttgt cgccgatgtt gagcatgtcg
     5761 gtggggtggt tgatcttgcc ccagctcatg tcggtgaggt ggaggaggcc gtcgatgccg
     5821 cccagatcga tgaaagcgcc gtattgggtt atatttttca cctcgccgtc aagctcgata
     5881 tccacttgga tcctggagag cagttcttcg cgtttgatcc
//
LOCUS       DAOOWR010000070         5899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13665_length_5899_cov_4.430014, whole genome shotgun sequence.
ACCESSION   DAOOWR010000070 DAOOWR010000000
VERSION     DAOOWR010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13665_length_5899_cov_4.430014"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PLB85_02745"
     CDS             complement(<1..1119)
                     /locus_tag="PLB85_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOU72274.1"
                     /translation="MPNKWIMAAIGLVLGFLIALVIYLIKRNSAFRLVSGAKEIAAEI
                     RESAQTDIETTKKAVILEAKDEWFKQKKVLEDEIKERQKELRFQEKKFNERLSSLDKR
                     LESMDKKEANMVEQEKRLKHKEEDISRKNKEAENLIGEQRTKLSEIAGLSREEALNMM
                     REELLLQARQVAANDAKLTIEQIKLDTSKKAAEILSTAIQRLAVDHVSESTVSVVSLP
                     SDEMKGRIIGREGRNIRTFEKASGVDLIIDDTPEAVVLSCFDPVRREIARLALEKLIS
                     DGRIHPGRIEDIINKTEKEMEQNLVKLGEKAVLETNIHNISGNLIKVLGRLHYRTSYG
                     QNVLKHSLEAAWICGILAAELGLDQEIARRAGLLHDIGK"
     gene            complement(1351..1623)
                     /gene="zapA"
                     /locus_tag="PLB85_02750"
     CDS             complement(1351..1623)
                     /gene="zapA"
                     /locus_tag="PLB85_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOU72275.1"
                     /translation="MMKSVEVEIYGRKFHLRSDNLKETQAVAAAVDAELEQLREQYEN
                     LDFTKLLLLLLLKKQNEISSLKQRSAEMEKELERLNQMVGNIVGEI"
     gene            complement(1620..1928)
                     /locus_tag="PLB85_02755"
     CDS             complement(1620..1928)
                     /locus_tag="PLB85_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72276.1"
                     /translation="MQDKIQKLINQYTEDKKKLEKLAGQNEVLKEENRQLMAQIESVR
                     NSNSDSSERIRELENQLASLQTKYQELQDTVSGFESIASDAISQIDSIFPELEETNKK
                     "
     gene            complement(1954..4479)
                     /gene="pheT"
                     /locus_tag="PLB85_02760"
     CDS             complement(1954..4479)
                     /gene="pheT"
                     /locus_tag="PLB85_02760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU72277.1"
                     /translation="MIISCRWLSRYIDLPRDTDSLVQTLTFSGIEVEAVKSLPALPET
                     VVTAKVVSAERIPGTDHLQVCSVEYGASEPTRVVCGAPNCKAGMISLLALPGTELSGL
                     TIKKAKLRGVESNGMLCSEKELGISDNHAGIIELEPSTPVGISVNDIYELPDTILELE
                     ITPNRPDLLGYIGIARDLSASLGNPLKLPLVEQVAGEVSEKTTLKLILEDPEKCPRYT
                     ARLLSGVSVKDSPQWLKTALVKSGLRPINNIVDVTNFVMLEQGHPLHAFDYDKLQPLN
                     ESDGHPAVVVRKARSGEEFITLDGKSLVLDPDDLVIADGKDTSALAGVMGGKATAISD
                     STTSIVLEAACFQAASIRASSYKHKLSTDSSYRFERQLSPEAASAVSDRAVQLFLETA
                     GGKVENALYDAWPKPLPTRWLALRPRRFTEIIGYQLQNEDIIGYLEGLGCKFYQYGIW
                     REGLVSDPSLIFCHHLKEEKAGVTEFSESDCDHALWFEIPPHRVDLTREADLLEELAR
                     LAGFDRITRKKDVPRIMDRHAFRIRRGIEDWFVDAAFHETLNYSFCDPAQLADLAMDP
                     EEVSSKLIRVINPQSSNQSAMRITLLPQLLDNLRYNLNHGERDLKLMELGKIYLKDGD
                     SYKEKLSFGAALTGNMNPEHWSAKSEAVNFYQVKGLVEGLLNLLGVKATNLVKNDRPW
                     LAAGDSLAYLSEGQAVASFGRLKTSIANNFGIDLTLLKQDIWVIELDVDLLIELTRDI
                     KKVFRELPRYPAVIRDLSFLIKTEVSYAAIESAIKAVNPDMISEVSIFDQYLGKQVPP
                     GFRSMSLRLVMQDNEKTLTDERVDQLLASVQEMLSRAWQINMR"
     gene            complement(4476..5510)
                     /gene="pheS"
                     /locus_tag="PLB85_02765"
     CDS             complement(4476..5510)
                     /gene="pheS"
                     /locus_tag="PLB85_02765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOU72278.1"
                     /translation="MQDQLREVAEQARTDIAACETANDILNAKARYLGKKSLVNSLYG
                     KLKDLPDAEKPAFGQSINKLRGSIEDLLAAREQELKNRLQEAQDSAKTLDLSLPGIIR
                     PRGSLHPLTIVRRRIEDVFIGLGFEIAEGPDIDDEWHNFDALNTPPDHPSRNLADTFY
                     LDNGFLLRTQTSTVQVRTMEKYPPPIYIISPGRCYRNDKPDPSHSPVFSQVEALVVDK
                     GISMADLKDTLQNFARIMFGEDVRSRIRPHFFPFTEPSAEMDISCVACRGQGCRVCKN
                     SGWLELGGAGMVDPAVFKYVGIDPEIYSGYALGVGVERIAMLTYNIPDMRILFENDVR
                     MLRQFSGEVL"
     gene            complement(5561..>5899)
                     /gene="rplT"
                     /locus_tag="PLB85_02770"
     CDS             complement(5561..>5899)
                     /gene="rplT"
                     /locus_tag="PLB85_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOU72279.1"
                     /translation="STNNVSAHRRRKKYMLAARGYFGRRSKTYRVARQTVERGMAFAF
                     AHRKQKKRQFRSLWITRINAACRNNDMSYSRFINGLHKANIEINRKSLAHLAWHDSDA
                     FAKLVEIAKG"
BASE COUNT         1117 a         1521 c         1708 g         1553 t
ORIGIN      
        1 tttgccgatg tcgtgcaata atccggcgcg acgggcaatc tcctggtcga ggccaagttc
       61 agcggcaagg ataccgcaga tccaggctgc ttccagcgag tgcttgagca cgttttgtcc
      121 gtaggaggtg cggtagtgca gacggcctag caccttgatc aggttgccgg agatgttgtg
      181 gatattggtt tccagcacag ctttttcgcc cagcttaacc aggttctgct ccatctcctt
      241 ttcggtttta ttgataatgt cctcgatgcg gccgggatgg atccggccgt cggagatgag
      301 tttttccaaa gccagacggg cgatttcacg gcgcacgggg tcaaaacagg aaaggacaac
      361 tgcttcggga gtgtcatcga tgatcaggtc taccccggag gctttttcaa aggtgcggat
      421 gttgcgtccc tcccggccga tgatgcggcc tttcatctcg tccgagggca gcgataccac
      481 cgatacggtg gattccgaaa catggtccac ggcaaggcgc tggatggcgg tggagaggat
      541 ctccgcggct tttttggaag tgtccagctt gatctgctcg atggtgagtt tggcatcatt
      601 ggccgccact tggcgggctt ggaggagcag ttcctcgcgc atcatgttaa gcgcttcctc
      661 gcggctgagc ccggcgatct cggagagttt ggtgcgctgt tcgccgatca ggttttcggc
      721 ttccttgttt ttacggctga tatcctcttc cttgtgtttg aggcgttttt cctgctcgac
      781 catgttggct tctttcttat ccatggattc gaggcgttta tccaggctgc tgaggcgttc
      841 gttgaatttc ttttcctgaa agcggagttc tttttggcgt tccttgatct cgtcttcaag
      901 cacttttttc tgcttgaacc attcgtcctt tgcttccaga atgacggctt ttttggttgt
      961 ttcaatgtcg gtttgggcgg attccctgat ctccgcggcg atttccttgg cgccggaaac
     1021 aaggcggaaa gcgctgttgc gtttgatcag gtagataacc aaggcgatga ggaagcccaa
     1081 caccaatccg attgctgcca tgatccattt atttggcatc ataatatcct ttttgatcta
     1141 ttcaggcggg ttacttaacc cggccgttac aaaaaaaggc ccgcgtgaac cgtgtatgct
     1201 tgtctgttaa aagccaagca tcaggtgggc gcctaccttg tgggctttat ggatccggga
     1261 ccagccggcg tgcatgctac ccatggctcg gctcccagtt ttttatcagc ttttgtgcaa
     1321 tccatatcac gggttccgca ggccaagtct ttagatctcc ccgacgatgt tgcccaccat
     1381 ctggttcagg cgttcaagtt ccttctccat ttcggcggac cgttgtttca gggagctaat
     1441 ctcattctgt ttcttcaaaa gaagcagaag caaaagtttc gtgaagtcaa gattttcata
     1501 ctgttcgcgc agttgttcca attccgcgtc aacagcggcc gccacggcct gtgtctcttt
     1561 caggttgtcc gaacgcaggt ggaatttgcg gccatatatc tcaacttcaa ccgatttcat
     1621 catttcttat tagtttcttc cagttccgga aagatactgt cgatctggct gatggcgtcg
     1681 ctggcgatgc tttcgaatcc ggatactgta tcttgaagtt cctggtattt ggtttgaagc
     1741 gacgcaagct gattctccag ttccctgatg cgttcgcttg aatcgctgtt gctgtttctg
     1801 acgctttcga tctgggccat cagttgtctg ttttcttcct tcagaacctc gttttgcccg
     1861 gccagtttct ccagcttttt tttgtcttcg gtgtactgat tgatcagttt ttgtatctta
     1921 tcttgcaagc ttgttgcttt attttccata gtgttacctc atgttgatct gccaggcgcg
     1981 ggagagcatt tcctgcaccg atgcaagaag ttggtccacg cgctcatctg tcaatgtttt
     2041 ttcgttgtcc tgcatcacca agcgcagaga catgctacgg aatcccggag gaacctgctt
     2101 tccgaggtat tggtcgaaga tgctcacttc gctgatcata tctggattta cggctttgat
     2161 ggcggattcg atggcagcgt aggacacttc ggttttgatc aggaatgaaa ggtcgcggat
     2221 cacagcagga tagcggggca attcgcggaa caccttttta atgtcgcggg tgagttcgat
     2281 cagcaagtcc acgtcgagct cgatcaccca gatatcctgt tttagcaggg tgaggtctat
     2341 cccaaagttg ttggcgatgc tggttttcag acggccgaac gaagccaccg cctgcccctc
     2401 tgaaaggtag gctagagaat ccccggcagc cagccaggga cggtcatttt tcaccaggtt
     2461 cgtagccttt actccgagca ggttcagtag cccctccacc aagcctttca cctgatagaa
     2521 attcacagct tcgcttttgg cgctccaatg ctcggggttc atgttgccgg tgagtgccgc
     2581 gccgaaggat agtttttctt tgtaggaatc gccgtccttc aagtagattt tgccgagttc
     2641 catcagctta aggtcacgct cgccgtggtt gaggttgtag cgcaggttat ccagcagctg
     2701 gggcagcagg gttatccgca tggcggattg gttgctgctt tgggggttga tcacacgtat
     2761 tagtttggag ctgacctctt cggggtccat ggccagatct gccagctggg ctggatcaca
     2821 gaagctgtag ttcagcgttt catggaaggc ggcgtccaca aaccagtcct caatccctct
     2881 gcggatgcgg aaggcgtggc ggtccatgat gcgggggacg tcctttttgc gggtgatgcg
     2941 gtcgaagcca gccaagcggg ccagttcttc cagcagatcg gcttcgcggg tgaggtccac
     3001 tcggtggggt gggatttcga accagagggc gtggtcacag tcgctttcag agaattcggt
     3061 aactcccgct ttctcctcct tcagatggtg acagaagatc aggctgggat cagagaccag
     3121 gccttcccgc cagatgccgt actggtagaa tttgcagccc aagccttcca gatagccaat
     3181 gatgtcttcg ttttgcagct gatagccgat gatctcggtg aagcgccggg gcctaagcgc
     3241 cagccagcgc gtgggaagag gttttggcca ggcatcgtaa agcgcgtttt caacctttcc
     3301 gccggcggtt tccaaaaaga gctgcaccgc gcggtcgctc accgcggaag cggcttcggg
     3361 gcttaactga cgctcgaaac ggtaggagga atcggtggag agcttgtgtt tgtaagaact
     3421 ggcgcgaatg ctcgcagctt gaaaacaagc ggcttccaga acgatggagg tggtggaatc
     3481 gctgatggcg gtcgcttttc cgcccatcac tccggcaagg gcggatgtgt ctttgccgtc
     3541 ggcgatcaca aggtcgtcgg ggtctaaaac cagactcttg ccgtccaggg tgatgaattc
     3601 ctcgccggac ctcgctttgc gcaccaccac ggcgggatgg ccatcggatt cgttcagcgg
     3661 ctgcagcttg tcatagtcga aagcgtgcag gggatgtccc tgctcaagca tcacaaagtt
     3721 ggtcacgtca acaatgttgt tgatgggacg caaaccggat ttgaccagtg cggttttcag
     3781 ccattgggga gagtccttca ctgaaacacc ggagagcaat ctggccgtgt agcgggggca
     3841 tttttctgga tcttccagta tcagtttgag cgtggttttt tcgctgactt caccagccac
     3901 ttgctccact agcggcagct tcaggggatt gcccaaagag gcggaaagat cgcgcgcgat
     3961 gcctatgtat cccagcaggt ctgggcggtt gggcgtgatt tccagttcca gaatggtgtc
     4021 cggcagttcg tagatgtcgt tcacggaaat ccccaccggt gtggagggct ccagttcaat
     4081 gatccctgcg tggttgtcgg agatgccgag ttccttttca gagcaaagca tcccgttgga
     4141 ctccacaccg cggagttttg cctttttgat ggtaagacca gagagttcgg ttcccggaag
     4201 ggccagcaga gagatcattc cagctttgca attgggtgct ccgcaaacca cccgggtagg
     4261 ctccgaagct ccgtactcca cgctgcaaac ttgcagatgg tcggttccgg ggatacgctc
     4321 ggcgctcacc actttggcgg ttacaacagt ttccggcaag gcgggaagag attttacggc
     4381 ttccacctca atcccactga aggtgagggt ttgaaccagg ctgtctgtgt cccggggcag
     4441 gtcgatgtag cggctgagcc agcggcagga gataatcaca atacctcccc gctgaactgg
     4501 cgcagcatgc gaacgtcgtt ctcaaagagg atgcgcatgt ccggaatgtt gtaggtaagc
     4561 atggcaatgc gctccactcc caccccaagg gcgtatccgg agtaaatctc gggatcgatg
     4621 cccacgtatt tgaatacggc gggatcaacc atcccggctc cgcccagttc cagccaaccg
     4681 gagtttttgc acacacggca accctggcct cgacaggcga cacagctaat atccatttcc
     4741 gcgcttggtt ccgtgaaggg gaaaaagtgt ggtcggatgc gcgaacggac atcttcgccg
     4801 aacatgatcc gggcgaagtt ttgcagcgtg tctttcaaat cggccatcga gataccttta
     4861 tccaccacta gtgcttccac ttgggaaaaa acgggggaat gggatgggtc cggcttgtcg
     4921 ttgcggtagc atcttccggg cgagatgata tagatgggcg gcgggtattt ttccatagtg
     4981 cgcacctgca cagttgaagt ctgggtgcgc aacaggaagc cgttgtccag gtaaaaggtg
     5041 tctgccaggt tacgggaagg atggtcaggc ggggtgttca gggcgtcgaa gttgtgccat
     5101 tcgtcatcga tatccggccc ttcggcgatc tcgaaaccca ggccgatgaa gacatcctcg
     5161 atcctgcggc ggacgatcgt tagcggatgc agcgacccac gcgggcggat gatgcccggc
     5221 aggctcaggt ccagcgtctt ggcgctgtcc tgggcttcct ggagacggtt tttcagctcc
     5281 tgttcacgcg cagccagcag gtcttctatg ctgccgcgga gcttgttgat gctctgcccg
     5341 aatgccggtt tctcggcgtc cggaaggtct ttcagctttc cgtagaggct gttcactaag
     5401 ctttttttgc caagataccg ggctttggca ttcaggatgt cattggcggt ttcgcaggcg
     5461 gcaatatccg tcctggcctg ttccgccact tccctcagtt ggtcttgcaa cttgcttcct
     5521 tcactatttt agctccggct gctcgggccg gaggggtggt tcagcctttg gcgatctcga
     5581 ccagtttggc aaaggcatcg ctgtcgtgcc aggccaagtg ggcaagggat ttgcggttga
     5641 tttcgatatt ggctttatgc aggccgttga tgaagcggct gtagctcatg tcgttattgc
     5701 ggcaggcagc gttgatgcgg gtgatccaga ggctacggaa ctggcgtttt ttctgcttcc
     5761 ggtgggcaaa ggcgaaagcc atgcctcgtt ccacggtttg cctggcaacc cggtaagttt
     5821 tgctgcggcg gccgaaatag ccgcgcgcgg ccagcatgta tttttttctt ctgcggtggg
     5881 cggatacgtt gtttgttga
//
LOCUS       DAOOWR010000071         5882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13708_length_5882_cov_3.397288, whole genome shotgun sequence.
ACCESSION   DAOOWR010000071 DAOOWR010000000
VERSION     DAOOWR010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13708_length_5882_cov_3.397288"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLB85_02775"
     CDS             complement(<1..860)
                     /locus_tag="PLB85_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72280.1"
                     /translation="MLAAALPAQPVREDDDFAYIVELYYSGDAYLDEVSAELETFGNR
                     YPDSNYSQYILYLKGNIALKRGEYDLSSSIYEDLLQQELHPDILADIQLNYAITNYYR
                     SDYPKCLKLLDELECSAVHPWYLYQVNVWRGRIKARQELWFSAAEEYQKALAVNAAEV
                     RFDYFQTLLALEREDQVTAILDTLSASSSDYMQFHGAWLDQLLSEGRYTEFEEHAAAL
                     DSLHSSSSVLTLLRTRKALELGESLDTKIWLELQTEPSDLATFYRALLLKQNGYIGPA
                     DSLFKSLLHSP"
     gene            complement(959..>5882)
                     /locus_tag="PLB85_02780"
     CDS             complement(959..>5882)
                     /locus_tag="PLB85_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72281.1"
                     /translation="GKRACYASDFYSLGVLIYRMLTGSFPFTLDQINAMRSSRRQYFS
                     PVYPVDLNPNIPLPLQNLCIELLDRNPESRPHKSEEIIEYINRSTNRQYQFSVSWSLV
                     NSMQFNSYVVQERIVNDLLDYLPQVEAANGKIISLVAGEGMGKDNILSLFRYHILRGS
                     YFIFDYTCTSKEYEAFFALIKEYLRSLSEDEIASNGSLQNISEKMRRYLFMSEEAAMD
                     VTPSEADLKADFEFAKALLTELAESKPVIFIVRNIQHLHRHSLEFLNFFSNTVVNNRI
                     MIVMSCTDFNQVRRIRNTVLINVPMFTPDESAAYIRKMVNGDVPNHFCRLIHTRSAGN
                     PFLIREIMINLTLRGMISYDGEYHFPADLDNYSIPTRLLQSVIRRVEHLTPASMKQLQ
                     KLSVVQTPISRELIRHICKARDEEIYDLLNEAKYNEILLKQGNHYHFTFAEAKTYLHD
                     RCQPKTRNLVSKRVLEFYDNQNVTDPQTCMGIIGNARTAGDRASERRYLLRLHLLHSE
                     DHLQQEAYDALAHALRIDFSGDIDLRQEEIVEDLVKFRRMVEITGCTTEAEFIYARQS
                     DIPECFEKYLLLGTLFLFQGDRKSALKNFEKAEKLSADASQKSTAKLYQAQIYARTEP
                     ARVKKCLDGIALDKVSQAFQIKAATLMAAYESRDRDYDRAARGLEDFLTELAPSQDPD
                     AMIELASLHNTLGEIYSIQKNIAEADEHFNTALNIWNSFNIRRHLGWIHNNLADLYLK
                     QGFTILGLKHAQQAQVFAKDRQNLLAEGRALLNQGEAMIKMGRFEKAEETLQEAKEMI
                     TRVGANKYLVSIERNLALAKSKISDFGHYYKFILEHEPKLIEGTISEINPLVKTYFYY
                     LSEMSNPKKLRRLISKNAHIDYAQIQEQEFYHNVLSLLAMMEKDYSTALNELKLALQF
                     AGEINNHYAMAVFNVLQATCHYGLGDTARAADLIAKARPAIQEYQYRYWEHSLDLLEL
                     KLQLTDPSIPLRKVLRGINRQLELCRGLKYYQLVEELLQLKIQLLVELGSLGAAEKEF
                     AAYRKYLEAITTGIREDDRTNYLEVKRYGLQDVSKFRVVPIASRRRNTRSRWNDMLFD
                     ISNVNSVQRVKFLIEKGIGQIIAPWKFLLLVWSDKLGSFYNFHSYNTDPETILPPAYL
                     PHVDKAIQSNNLVTFTNEREHIAILPLLSGSKAIGYLVLSDAGELEFTPGEQALLKNV
                     KSHLAAMLVRTWDYQKINLRMEKMNHLIEISHELMGLMDLNDLETAMVAVAIDLTNAS
                     RGFLIKKDTDGNNIFQVQMDHNGQILNNAFGVSKTALNLCQDGQKTISVYNAQLDKSY
                     ENSISVHDYAIQSIFCSPLRWEEEYVGYLYLDNLDDSTRDMYLNEDIMVLLMNLFANA
                     VKNVLQYSALTQKSAELNNLEQLKDEFIAIVTHELNTPIFIMQGALGKLKRSANLDET
                     QRRQILSELEAAMNKLSLTSSDIQTMNHYNLAQDLEKSPLRVADVLEHIHQQVEIISR
                     ERRMHIRLEIEPGLPPLQSNWTDFHRMVYNVVLNAVRFTREFGLITIGARRSAFVQEK
                     IDNKETLVIFVTDNGMGMPKHLINEVFRKFYELNAIYAHKSGTVEYRSSGLGLGLSIS
                     KRIAELHGGQIVIKSKENEGTSVFIILPFK"
BASE COUNT         1267 a         1429 c         1638 g         1548 t
ORIGIN      
        1 ggggaatgaa gtagggattt gaacagggaa tcggctggac ctatgtaccc attctgtttc
       61 aacagtagcg cgcggtagaa ggtggccaag tcagaaggtt ctgtttgcag ttccagccag
      121 attttggtat ccagactctc acccagttcc aaggctttgc gcgttcgcag cagagtcaat
      181 accgatgaag aggaatgaag gctgtccaga gcggcggcgt gttcctcaaa ctcggtgtag
      241 cgaccttctg aaagaagctg gtcaagccag gctccgtgaa attgcatgta gtctgatgag
      301 gaggccgaaa gcgtatccag gattgccgtg acctggtcct cgcgctccaa agcaagcagg
      361 gtctggaaat aatcgaaccg gacctcggca gcgttcactg ccagggcttt ctgatattct
      421 tcagcggcgg aaaaccatag ttcctgtctg gctttgatgc ggccccgcca aacattcacc
      481 tgataaagat accagggatg gaccgcagaa cactccaatt cgtcaagcag cttcaaacac
      541 ttggggtaat cgctgcgata gtagtttgtg atggcataat tgagttgaat gtcagccaat
      601 atgtctgggt gaagttcctg ctgcagtaaa tcttcgtaga tgctactgct gaggtcatat
      661 tctccacgct tgagggcgat atttcccttc aggtaaagga tgtactggct gtaattggaa
      721 tccggatagc ggttgccaaa ggtttccagt tccgccgaaa cctcgtccag ataggcatcg
      781 ccgctgtaat agagttcaac gatgtaggca aagtcgtcgt cctcgcgaac tggctgggct
      841 ggcaaagccg cagcgagcat tatcaggatg aacaggacca gactaaaacg cggcagggaa
      901 caaaatccac aagttggttt tgcgtgtatg tgttggtgca tattattata catcccgctt
      961 atttgaaggg taggatgatg aacacggagg tgccttcgtt ttccttgctt ttgatcacga
     1021 tctgtcctcc gtgcagttca gcgattcgct tggagatgga gagccccaaa ccaaggccgc
     1081 tgctgcgata ctcaactgtt ccgctcttgt gggcgtagat ggcgttcagc tcgtaaaact
     1141 tgcggaacac ttcgttgatc aggtgtttgg gcattcccat gccgttgtcg gtaacaaaga
     1201 tcaccagggt ttccttgttg tctatctttt cctgcacaaa ggcggaccgg cgtgccccga
     1261 tggtgatcag gccgaattcc cgggtgaagc gcacggcatt cagcacaaca ttatagacca
     1321 tacggtggaa gtctgtccag ttggattgga gcggaggcaa gccaggctct atctccaatc
     1381 tgatgtgcat tctgcgctca cgggaaatga tttccacctg ctggtgtata tgttccagca
     1441 catcggcaac ccgcagaggg gatttttcca aatcctgggc tagattgtaa tggttcatgg
     1501 tctggatgtc tgaagaggtt aaactcagct tgttcatcgc tgcttccagt tccgacagta
     1561 tctgccgtcg ctgtgtctca tccagattgg cactgcgttt tagcttaccc agggccccct
     1621 gcatgatgaa gatgggtgtg ttcagttcat gggtcacaat ggcgatgaat tcgtccttga
     1681 gctgctcaag gttgttaagt tccgcgcttt tctgcgtcaa ggcggagtat tgcagcacgt
     1741 ttttgacggc gttcgcgaag agattcatca gcagcaccat gatatcttcg ttcagataca
     1801 tgtcacgggt gctgtcgtcc agattgtcca gatagagata gccaacgtat tcctcttccc
     1861 acctgagggg gctgcagaaa atagattgga tagcgtaatc gtgcacgctt atggaatttt
     1921 catagctttt gtcgagttgg gcgttgtaaa ctgatatggt tttctgcccg tcctggcaga
     1981 ggttgagggc ggtcttgcta accccgaagg cgttgttgag gatttggccg ttatggtcca
     2041 tctgcacttg aaatatattg ttgccgtcgg tatctttctt gatcaggaag cccctgctgg
     2101 cgttggtgag gtctatggca accgccacca tcgcagtctc caggtcgttc aggtccatca
     2161 gtcccattag ttcatgcgaa atctcgatga ggtggttcat tttctccatg cgcagattga
     2221 tcttttgata gtcccaggtg cggaccagca tggcagcgag gtgggatttc acgtttttca
     2281 gcaaagcctg ttcgccagga gtgaattcca gttctccggc atcgctcaga accagatagc
     2341 cgattgcttt gctgccggag agcaggggca ggattgcaat gtgctctctc tcattggtga
     2401 aggttaccag gttgttggat tgaattgctt tatcgacgtg tggcaggtaa gccgggggca
     2461 ggatggtctc cggatcggtg ttatagctgt ggaagttgta aaagcttccc agtttgtccg
     2521 accataccag cagcagaaac ttccagggcg cgatgatctg gccgatgcct ttctcgatga
     2581 gaaatttcac cctctgcacg ctgttcacgt tggaaatgtc gaacagcatg tcgttccagc
     2641 ggctgcgtgt gttcctgcgg cgcgaagcta tgggcaccac cctgaatttt gaaacgtcct
     2701 gcagcccgta gcgcttcacc tccaaatagt tggtccggtc atcttctctg atgcctgtgg
     2761 tgatggcttc cagatattta cggtaggcgg caaattcctt ctcagccgct cccagggagc
     2821 ccagttccac taaaagctgg atcttcaact gcagaagttc ctcaaccaat tgatagtatt
     2881 tcaagccgcg gcacagttcc aactgcctgt tgataccgcg caaaaccttt cgcaggggga
     2941 tggatggatc ggtcaattgc agcttcagtt ccagcaggtc aaggctatgt tcccaatagc
     3001 ggtattggta ttcctggatt gccggcctgg ccttggcgat caggtctgca gcccgtgctg
     3061 tgtcgcccag accgtagtgg caggtggcct gcagcacatt gaaaactgcc atcgcgtagt
     3121 ggttgttgat ctccccggca aattgcaggg ccagcttcaa ttcgttgagg gcagtggagt
     3181 aatccttctc catcatcgcc agcaggctga ggacgttgtg gtaaaattcc tgttcctgga
     3241 tctgggcata gtcgatatgg gcgtttttgc tgatcagccg gcgcagcttc ttgggattgg
     3301 acatctcgct gaggtagtag aaataggttt taaccagcgg attgatctct gaaatcgtgc
     3361 cttcgatcag cttgggttcg tgttcgagga taaatttgta atagtggccg aaatcgctga
     3421 tcttgctttt ggccaaggcc aggtttcgtt cgatggaaac caggtatttg tttgccccga
     3481 cccgggtgat catttccttt gcttcctgca gtgtttcttc cgctttttca aagcgaccca
     3541 tcttgatcat cgcctcgcct tggttgagga gtgcgcgtcc ctcagccagc aggttctgac
     3601 ggtccttggc gaagacctgt gcctgctgcg cgtgtttaag gcccaaaatg gtgaagccct
     3661 gcttcaggta aaggtcggcc aggttgttgt ggatccagcc caagtggcgg cggatgttga
     3721 aggaattcca gatattcagg gcggtgttga aatgctcgtc ggcctcagca atgttcttct
     3781 gaatgctgta gatctctcct aacgtgttgt gtagcgaggc cagttcgatc atggcatccg
     3841 ggtcctggct gggcgccagt tcggtcaaaa aatcctccag tccccgggca gcgcggtcat
     3901 aatcacggtc acggctttcg taggcagcca tcagggtcgc cgcctttatc tggaatgctt
     3961 gtgacacttt gtccagagct atgccgtcca ggcatttctt cacccgggca ggctccgtgc
     4021 gggcatagat ctgtgcctgg taaagtttgg ctgtggactt ctggctggca tcggctgaaa
     4081 gtttctccgc tttctcaaag ttctttagag cgctcttgcg gtccccctga aagaggaaca
     4141 gcgttcccag caataagtat ttttcaaagc attcgggaat gtcggactgg cgcgcgtaga
     4201 taaattccgc ttcggtggtg cagccggtga tttcaaccat acggcggaat ttcaccaagt
     4261 cttccacaat ctcttcctgc cgcaaatcaa tatccccgct aaaatctatt ctcagagcat
     4321 gcgccaaggc atcataagct tcctgctgga ggtggtcttc gctgtgcaac agatgcagcc
     4381 gcagcagata acgccgttcg gaagccctgt cacctgctgt gcgtgcgttt ccgattatcc
     4441 ccatgcaggt ttgagggtcg gtcacgttct gattatcata aaactccaga acccgcttcg
     4501 agaccagatt tcgcgtcttt ggctggcagc ggtcgtggag ataggttttg gcctcggcaa
     4561 aggtgaaatg atagtgattg ccttgtttga gcaggatttc gttgtatttt gcttcgttga
     4621 ggaggtcgta gatctcttcg tcccttgcct tgcagatatg acggatcaat tcacgggaga
     4681 tgggtgtctg cacgacggac agcttctgaa gttgcttcat gctggccgga gtgaggtgtt
     4741 ccacgcgcct gataacggat tggagcaggc gggtggggat gctgtagttg tcaaggtcag
     4801 ccgggaaatg gtattcaccg tcatagctga tcattccccg cagggtgagg ttgatcatga
     4861 tttccctgat caggaaggga ttacccgccg agcgggtatg gatcagcctg caaaagtggt
     4921 tcggaacgtc cccattaacc attttgcgga tataggcggc cgattcatcc ggcgtgaaca
     4981 tgggaacatt gatcagcacc gtgttccgaa tccgccgcac ctggttaaag tctgtgcagc
     5041 tcataacgat catgatccgg ttgtttacca cagtgttgga aaagaaattg agaaactcca
     5101 gggaatggcg gtgcagatgc tggatattgc gcacgatgaa gatcaccggc tttgattcgg
     5161 caagctcggt gagcagggct ttggcaaatt cgaagtcagc cttaaggtcc gcctcgctgg
     5221 gcgttacgtc catggctgcc tcttcggaca tgaagagata ccgcctcatt ttttccgaaa
     5281 tgttttgcag cgagccattg gaagcgatct catcttccga aagggaacgc aggtattcct
     5341 ttatcagcgc gaaaaaagcc tcatattcct tgctggtgca ggtgtaatcg aagatgaagt
     5401 agctgccccg caggatgtga tagcggaaca gcgaaagaat attgtcttta cccatgcctt
     5461 cgcccgccac cagcgagatg atcttgccgt tggctgcctc aacttgcggc agatagtcca
     5521 gcaaatcgtt cactatccgc tcctgcacca catagctgtt aaactgcatg gagttcacca
     5581 aggaccagga gacggagaat tgatactgac ggttggtgct gcggttaatg tattcgatta
     5641 tttcttcgct tttatgggga cggctttccg gattgcggtc cagcagttcg atgcagaggt
     5701 tttggagagg cagtgggatg ttcgggttca ggtccacagg ataaaccggc gagaaatatt
     5761 gccggcggct gctccgcatg gcgttgatct ggtcaagtgt aaagggaaaa ctgccggtga
     5821 gcatgcggta gattaacacg cccaaagagt aaaagtcgct cgcgtaacag gccctcttgc
     5881 cg
//
LOCUS       DAOOWR010000072         5882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_13711_length_5882_cov_3.216921, whole genome shotgun sequence.
ACCESSION   DAOOWR010000072 DAOOWR010000000
VERSION     DAOOWR010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13711_length_5882_cov_3.216921"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLB85_02785"
     CDS             <1..357
                     /locus_tag="PLB85_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU72282.1"
                     /translation="FLLLLFFMVSTVFVQERVFWVQRDRFPFASENIDRVSRNESATI
                     YITRKEDILIDDFPRSVEDDSIVTTMKAKAREIPTLQVCFRTDRDTRYQAVRDVMMRL
                     QDADTRNVVFEVQRKL"
     gene            368..1018
                     /locus_tag="PLB85_02790"
     CDS             368..1018
                     /locus_tag="PLB85_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU72283.1"
                     /translation="MAAKIIDWKDHANAQFSKALALAITLILFGFLVMPRMESQQSKE
                     ESALAEMAEAPPDQREKEEDVQEQEIEISIPVFSEELATEDTPELAEQRAQALQIFGD
                     YQATTSTTLQVDEDEMPPGFEPYEDAPVPISSINPAYPAFAKKAGVQGTVVLEVEVYK
                     DGRVGNVEVRKSVQSGAGGLDEAAIAAVKAARFQPGKMNGNPVNTLVILDVEFKLN"
     gene            1038..4124
                     /locus_tag="PLB85_02795"
     CDS             1038..4124
                     /locus_tag="PLB85_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72284.1"
                     /translation="MKFNKLLFCLILALLFSGTAFAAMAESSSGSKKLDLSLYHNVGN
                     IWLRVSNYGFFGSGDDVVPQYPSLEYPGGSGIDYLYQGALWFGAKKYRRDDAGRKLYW
                     KALNPSADSTATVAEGTPDWQPWMKPVVDTLVTVGFDGDLDLYEFLPAYNPLVAGNAD
                     AVDMYNAYNALDGIATASTRYQKRGVDDDGDGIIDEDFVGYTFPLRAAHELPVQFQSF
                     GGQYIADTNNYNIINEGTNSEIWFPLGFMELSDTSHSTYDFSNPYDDDGDGQVDEDGA
                     PVSEQDFISYYYDYCPLGTVGDRDLGQSRSRNRHYPLNVRVRQMSYQWSYDYIKNMVY
                     VEFNITNMNIATQDTLFDCAMGIYMDCDVGPQSMGPEKAADDKSGYVKGTGYEFAYTY
                     DADGDGGLTTGRVGARVCTPDPEQLQFHCWYWKVGDGPDDFNPYSWNFSPRRTANEKY
                     WLLTGRNPDESKYTALRPEQIDVMEWEQPTPNDTRFLFAFYGDQEGMTDPSESSWNLP
                     PGKTMKIVIAVFPGENQEDLKASAQWAKEIYDRAQTLTTVVLPDTFPHYNPPEPPEIP
                     KLFAEMADNGNRIDLYWDNRSEFSYDTKTVSSGKIGWQNPTSHNLIPGLDSDPYAVDW
                     SDYPEEFQPPALSDSLAFNMNATVNPFTGHRLRHDFQGYATWGRSGSGSREDWILMER
                     WDKIETPQDLLDYQVNFSHPADSLHWNYGGYLGIDKGLPNRNAWTKTEDYSLYTAFDQ
                     NYLYTSNPDDLFWGWPIYDPHKEWSVETKAQAEAIAEEYDGCPDFVIKAMQARIFKHP
                     EMRPEIFDELYDSKMIPLPGHGGQVNINVNDPDDPEYTDRMAKLKTQRLARRYYHSAI
                     MYPRKGVEYYIAVTAFDRGIPENDLNYLETGRDADANMKIFFPGTLAQESMDNIYVVP
                     NPYIGRSTFDGRRDNDEKGDKSRRLWFINLPQECKIRIYTLAGDLVQELDHNGAYQAD
                     IITVSKAATKGISASGMHDWNLLTKHNQIIAPGVYLYSVQNKADNKIKVGKFVIIK"
     gene            4135..5166
                     /locus_tag="PLB85_02800"
     CDS             4135..5166
                     /locus_tag="PLB85_02800"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU72285.1"
                     /translation="MKKQTLLIIVLALMLLPGLLSAKPFGKVGTAALQFLKLGVDARA
                     IGMGEAYTAVTDDVSSVYWNPAGLAPAFENQVLASHTNWPAGIMHDFVAGTYTNGPST
                     FAAYGSVLHMDKMDITDEETFGPTGEQFTNSSLAFGLNYAQQFTNKFSAGIGVKYLRE
                     NLYTFSVNSYAFDIGSIYNTGFRNIKIGMALRNFGPDIRYLVDDDEDGAIDEDPFDLF
                     DNDGDGLIDEDGPELDSKIPMSFSLGICGDIMRTETSHWIVSAQLDNVVDRMETWNIG
                     TEYKMGNLYLRGGYQINYDANGFSAGVGYQIPTRFAIINIDYAYTDMGALAETFLKSA
                     HRLSIKFRY"
     gene            5199..>5882
                     /locus_tag="PLB85_02805"
     CDS             5199..>5882
                     /locus_tag="PLB85_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72286.1"
                     /translation="MKKTLLVILTLGFMLSFAFAKEMQPATNLKEMPVLMEDKVPVDP
                     STVYPVVQTRTAPEYTFTKLPTAIITNYYDYMIGSYNGLPLRNIPDTAGGGYFMSYHG
                     RRQPTSTRRCFYTYIDANGDVVNNNEITSVQNHEGYCTTAVDPISGKPFYAWHCDKDA
                     DAEYEVEVATDAFIAGISGLFNDTQIAIDNPTTITSPSGITTDTNEFIWPTAQIGPSP
                     VAGKRRVYMA"
BASE COUNT         1492 a         1703 c         1463 g         1224 t
ORIGIN      
        1 tttttgctgt tgctcttctt catggtttcc acggttttcg tgcaggaacg tgtgttctgg
       61 gtgcagaggg acaggttccc ctttgcctcg gaaaacatag atcgtgtatc ccgcaacgaa
      121 tccgcgacca tatacatcac tcgtaaggag gatatcctga tcgacgactt tccgcgctcg
      181 gtggaagatg attccattgt taccacgatg aaagccaaag cccgggagat ccctaccctg
      241 caggtatgtt tcagaacgga cagagacacc cgctatcagg ctgtgcgtga cgtaatgatg
      301 cgccttcagg acgctgacac ccgcaacgtt gtctttgaag tgcaaagaaa gctataagag
      361 gtgaaatatg gctgccaaga taattgactg gaaagaccac gccaacgccc agttcagcaa
      421 agcgctggcc ttggccataa cccttatact gttcggcttc ctggttatgc caaggatgga
      481 atcgcaacaa agcaaagaag aatccgcgct ggctgaaatg gctgaagcgc caccggacca
      541 gcgcgaaaaa gaagaggacg tgcaggaaca ggagatagag atcagcatcc ccgtgttcag
      601 tgaagaactg gccactgaag acactccgga actggctgaa caaagagccc aggctctgca
      661 gatctttgga gactaccagg ccaccaccag cactaccctg caagtcgatg aagacgaaat
      721 gcctcccggc ttcgagcctt atgaagatgc ccccgttccc atcagtagca tcaatcctgc
      781 ctatcccgcc tttgccaaaa aggccggtgt acagggaaca gtggtgttgg aagtggaggt
      841 ttacaaagac ggccgggtgg gcaatgtgga agttcggaaa tcggttcagt ccggagccgg
      901 tggattggat gaagcggcga tcgccgccgt caaagccgcc cgcttccagc ctggcaagat
      961 gaacggaaac ccagtgaaca ccctggttat tctggacgtt gagttcaaat taaattaaga
     1021 tatattggga gtattgcatg aagtttaaca aattattgtt ctgcttgata ctcgcgttgc
     1081 tgttctccgg aactgctttc gcggcaatgg cggaatcttc atcagggtcc aagaaacttg
     1141 acctttccct ataccataat gtgggtaaca tttggctgcg ggtgagtaac tacggtttct
     1201 tcggctccgg agacgacgtg gtacctcaat acccgtcgct ggagtaccct ggtggcagcg
     1261 gtatcgatta cctataccag ggcgcgctct ggttcggggc caagaaatac cgccgcgacg
     1321 acgccggccg caagctctac tggaaagcgc tcaatcccag cgccgacagc accgccacag
     1381 tcgcggaagg cacccctgac tggcagccct ggatgaaacc ggtggtggac actctggtca
     1441 cggttggctt tgacggtgac ttggacctct acgaattcct gcccgcctac aatcctctgg
     1501 tggccggaaa tgcggatgcc gtggacatgt acaacgccta caatgccctg gatggaatcg
     1561 cgaccgccag tacccgctat cagaagcggg gtgtggatga cgatggagac ggcatcatcg
     1621 acgaggactt tgttggctac actttcccgt tgagagccgc gcacgaattg cccgtccagt
     1681 tccagtcctt cggcggacag tacatcgccg acaccaacaa ctataacatc atcaatgaag
     1741 gaaccaattc cgaaatctgg tttcctttgg gcttcatgga gttgtccgac acctctcatt
     1801 ccacctatga cttttccaat ccttatgacg acgacggaga cggccaggtg gatgaggacg
     1861 gggctcctgt ttccgagcag gacttcatct cctactatta cgattattgc ccccttggca
     1921 cagttggaga cagggacctg ggtcagtcca gaagccgcaa ccgccattat ccgctgaacg
     1981 tgcgcgttcg ccagatgtcc taccagtgga gttatgacta catcaagaac atggtgtatg
     2041 tggaattcaa cattaccaat atgaatatcg ccacccagga caccctgttt gactgcgcca
     2101 tgggtattta catggattgc gacgttggtc cccagagcat gggccccgag aaagccgcgg
     2161 acgataaatc cggatatgtg aaaggtacgg gctacgaatt tgcctacacc tatgatgccg
     2221 atggcgacgg cggcctgaca accggtcgcg ttggcgcccg tgtttgcacc ccggaccccg
     2281 aacagcttca attccattgc tggtactgga aagtggggga cggcccggat gacttcaacc
     2341 cctacagttg gaacttctca ccgcgacgca cggccaatga aaaatactgg ctgctgacgg
     2401 gtagaaatcc cgatgaatca aaatatacag ccctccgccc cgagcagatc gacgtgatgg
     2461 aatgggaaca gcccactccc aatgacaccc ggtttctgtt cgctttctac ggtgaccagg
     2521 aaggcatgac cgacccatcc gaaagctcct ggaacctgcc tcccggcaag accatgaaga
     2581 tcgtgatcgc tgtattcccc ggtgaaaatc aggaagacct caaagcctca gcgcagtggg
     2641 ccaaagaaat ctacgacaga gcgcaaactc tcaccaccgt ggtattgcca gacacgttcc
     2701 ctcattacaa tcctcccgaa cccccggaga ttcccaagct ctttgcggaa atggccgaca
     2761 acggcaaccg tatagacctc tactgggaca accgcagtga attctcctat gacaccaaga
     2821 ccgtttccag tggaaagatc ggttggcaga accccaccag ccacaatctg atccccggct
     2881 tggattctga cccgtacgcg gttgactgga gtgactatcc cgaagaattc cagcccccgg
     2941 ctttatccga ttccctcgcg ttcaacatga acgccactgt caatccgttc acaggccata
     3001 ggctacgcca cgatttccag ggatatgcca cctggggccg aagcggcagc ggatcacggg
     3061 aagactggat cttgatggag cgctgggata agatcgaaac tccgcaggat ttgctggatt
     3121 accaagtgaa cttctcgcat ccagccgatt cactgcattg gaattacggt ggttatctgg
     3181 gaatagacaa aggccttccc aaccggaacg cctggactaa gaccgaagat tacagccttt
     3241 ataccgcttt cgatcaaaac tacctataca ccagcaatcc cgacgatctc ttctggggtt
     3301 ggcccatcta cgatccccac aaggagtgga gcgtggaaac caaagcccag gctgaggcca
     3361 tcgcggagga atatgacgga tgtccggatt tcgtgatcaa agccatgcag gcacgcattt
     3421 tcaaacatcc cgaaatgcgg ccggaaatct ttgacgaact ctacgactcc aagatgatac
     3481 cgcttccagg gcatggtgga caggtcaata tcaatgtaaa tgatccggat gatccggaat
     3541 acactgatcg catggcaaaa ttgaaaaccc agcggctggc ccgcaggtat taccactccg
     3601 ccattatgta tccccgcaaa ggtgttgaat actacattgc cgtaacagcc ttcgaccgcg
     3661 gcattcctga aaacgacctt aactaccttg aaaccggacg cgacgccgat gccaacatga
     3721 agatcttctt ccccggcacc cttgcccagg aatccatgga taatatctat gtggtgccaa
     3781 atccctacat cggcagaagc accttcgacg gccgccgcga caatgatgaa aaaggcgaca
     3841 aaagccgccg tctctggttc ataaacttgc ctcaggagtg caagatccgc atctacaccc
     3901 tggctggcga cctcgttcag gaactggatc acaacggcgc ataccaggct gacatcatca
     3961 cggtttccaa agccgcgacc aagggcatct ccgccagcgg catgcacgat tggaacctgc
     4021 tcaccaagca caatcagatc attgcgcccg gagtgtatct gtactccgtg caaaataaag
     4081 ccgacaacaa gatcaaggtc ggcaagttcg tcatcatcaa ataggggagg aacagtgaaa
     4141 aaacaaaccc tactgatcat cgtgctggct ctgatgctgt tgcccggcct gctttccgcc
     4201 aagcccttcg gcaaagtggg aacggccgcc ctgcaattcc tcaaactggg tgtggacgcc
     4261 cgcgcaatcg gcatgggcga agcctacacc gcggttactg acgacgtttc ctctgtttac
     4321 tggaaccccg ccggacttgc ccctgccttc gaaaaccagg tgctggcctc tcatacgaac
     4381 tggcctgccg gcattatgca cgatttcgtg gcaggcacct atactaacgg cccctccacc
     4441 ttcgctgcct acggttccgt gctgcacatg gacaagatgg acatcaccga cgaggagaca
     4501 tttggtccca ccggtgagca attcaccaac agcagcctgg ctttcgggct caactacgcc
     4561 cagcagttca ccaacaagtt ctccgcgggc atcggagtta aatacctgcg ggaaaacctc
     4621 tacaccttct cggtgaacag ctatgccttc gatatcggct ccatctacaa taccggattc
     4681 agaaacatca agatcggcat ggcccttcga aatttcgggc cagacatccg ctatttggtc
     4741 gatgacgatg aagacggcgc catcgatgag gatccttttg acctcttcga taatgacggt
     4801 gacggcttga tcgacgaaga cggaccggaa ctcgattcca aaatccccat gagcttctct
     4861 ctgggaatct gcggagacat catgcgaacc gaaacttctc actggatcgt ttcggctcag
     4921 ctcgacaacg tcgtcgaccg catggaaacc tggaacatcg gcacggaata caagatgggc
     4981 aatctctatc tccgtggcgg ctaccagatc aactacgatg ccaacggctt cagcgccggg
     5041 gtcggatatc agatccccac ccgctttgcc atcatcaata tcgactatgc ctacacggac
     5101 atgggcgcac tggccgaaac cttcctcaaa agcgctcaca gactgtcgat aaaattccgc
     5161 tactaaccaa aatgactcga taaataatgg aggtaagaat gaaaaaaacc ttattggtca
     5221 tattaacgct tggcttcatg ctctcctttg ctttcgctaa ggaaatgcag cccgctacca
     5281 acctgaagga aatgccggtt ctcatggagg acaaagtccc tgtggatccc agtaccgtgt
     5341 atcccgttgt gcaaacccgc actgctccgg agtacacctt caccaagctg cccaccgcga
     5401 tcatcaccaa ctactacgac tacatgatcg gtagttacaa cggcctgccg ctgcgtaaca
     5461 tccctgatac cgccggcggc ggatatttca tgagctatca cggccggcgc cagcccacca
     5521 gcacccgccg ctgtttttat acctatatcg atgccaacgg cgatgtggtg aacaacaacg
     5581 aaatcaccag tgtgcagaat cacgaaggtt actgcaccac ggcagtcgat cccatatccg
     5641 gaaaaccatt ctatgcctgg cactgcgaca aggacgcgga tgccgaatac gaagttgaag
     5701 tagccaccga cgctttcatc gcaggcatct caggcctttt taatgatacc cagatcgcca
     5761 tcgacaaccc cacaaccata acttcaccca gcggaattac caccgacacc aacgagttca
     5821 tctggcccac cgcgcagatc ggaccctcac ctgttgccgg caagagaagg gtgtacatgg
     5881 ca
//
LOCUS       DAOOWR010000073         5766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14078_length_5766_cov_3.077745, whole genome shotgun sequence.
ACCESSION   DAOOWR010000073 DAOOWR010000000
VERSION     DAOOWR010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14078_length_5766_cov_3.077745"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="ruvA"
                     /locus_tag="PLB85_02810"
     CDS             <1..471
                     /gene="ruvA"
                     /locus_tag="PLB85_02810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOU72287.1"
                     /translation="CKIYTHMHLAQDDLRLFGFASPAERELYQQLNRISGVGPKSALS
                     IISTLPIPTFVKAIEREESALLTKVPGIGMKSAQRLIIELKGKLRHLMDYAEPGQLGV
                     EEDKVTEVENALQTLGFSPKDVRRELSLMGEEAQAQATEQLIKEVIRRLYQRSR"
     gene            472..1785
                     /gene="rsmB"
                     /locus_tag="PLB85_02815"
     CDS             472..1785
                     /gene="rsmB"
                     /locus_tag="PLB85_02815"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOU72288.1"
                     /translation="MVRKEAYQTILRIFKDKEFSDTLLHQRAKRLKSDRVNVALFYNM
                     VKGVVKLRGKLDYILSQHADEAKFAATDIKIKIWLYLGLYQLIYLDSIPAHAAINETV
                     KLAKENLGDKVGDFVNAMLRSWQREPEVVYPTDPVQRIACEHSYPPELIEQWIDLWGE
                     EDAEYLAIWFNENPKLHMRVNGTATTPEKLIQYFAKRDIKVTPSPVGKMTLLTEEADS
                     VLDDVAFSEGYFSIQDTSSSLIVELLDPQPEENILDLFAAPGGKCSYIAEILNNTGEV
                     VAVDKIPSKMKLLKQAADRLQLSNIKPIVSDAFKYGPVAPAYDRVLVDAPCSGWGVFG
                     RKADLRWQAHQNIEELVKLQEKALALGANFVRPEGIMVYSTCTMNPEENERQIEKFLA
                     RNPRFTLIDASEKLPGDFCQGGMFRSLPFRHNMDGAFAAKMIKTR"
     gene            1792..2388
                     /locus_tag="PLB85_02820"
     CDS             1792..2388
                     /locus_tag="PLB85_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU72289.1"
                     /translation="MASEISKKFWLTVGIGAGIIFLSAFITSQIIFPIIFREPKQIEV
                     PNLVGISSAAARQTLSALGLHAVVKDSIWSETERIDTVLEQNPAGGALIKPEGTVYLR
                     VSRGTRKVGVPSVVGLSYQEAYYTLVNAGLKGVVADSLYSDSYAPNTVIRCIPGVGSK
                     IELGAVVRMYMSRGPEPVIQGGAFADTTLQYETPFENW"
     gene            complement(2645..3412)
                     /locus_tag="PLB85_02825"
     CDS             complement(2645..3412)
                     /locus_tag="PLB85_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOU72290.1"
                     /translation="MTLIVSIIIAVLGAVLGSFFNVLIDRIPRRESVVNPPSHCTSCG
                     KQLPARQNIPIYTYLAQKGKCKFCGAKIHWHHLAVEIATPLLFLALSFVYGIASFSFW
                     KFAVMFGFLIPIFFIDAMHKIIPLVLSLPMVAVGMLLSFIQSGFNFVDFFWVYLLPAL
                     GLFALLYLLALGWEKFFHKEGLGGGDVILIPAVAAFFGVIHIPFVILLACLMGILYFL
                     LFIRKPEQAFAFGNFIALAAVIWALAGETLMRSTGLI"
     gene            complement(3419..3874)
                     /locus_tag="PLB85_02830"
     CDS             complement(3419..3874)
                     /locus_tag="PLB85_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72291.1"
                     /translation="MKKTLIGILILLLSLPLLAQIDMPKPNLNPYKGSGTPLFGLNKL
                     SMSHSLGFEAGTSSSGRGYYLSRYTNHINYAFNPKLDLELDLSLVNYGSTSKLQFNSD
                     NNTKVIPEFRLNYRPSDSVNLSVEFGQGTLWYTGPSNWYSDQRPRYERW"
     gene            4475..4957
                     /locus_tag="PLB85_02835"
     CDS             4475..4957
                     /locus_tag="PLB85_02835"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOU72292.1"
                     /translation="MKQTDLTQAYGLVHLPAKVALAVTENPGGGYNLITLEWFMRTSI
                     QPPMFAISVGHTRFSYECLQENRFFNLVFPSAEMKPLCALAGSTSGRDTDKFKKGGVE
                     SIPGKYRRLPVPREAVAVFECEVTSQVRSGDHTIFVGEVRQCWANESKELLVYSGKEL
                     "
     gene            complement(4977..>5766)
                     /locus_tag="PLB85_02840"
     CDS             complement(4977..>5766)
                     /locus_tag="PLB85_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72293.1"
                     /translation="LIIAGSSTGNLVLGGANIGSKPDGAGNVIFISRLGADGTWNWGS
                     RLQQLSTDCFRKCFDTDAAGNTWFADNYYGNPIPNPIYDGALSVNSSLSLVDSQGRKL
                     WTHSLEGTDVSIDWISSGRQKDLFVAGTFSGSLSLGSQKLSAGGHKAQFYARFDADGK
                     CLWARKWLGTDLLFLDNDLAGNLYLVAKYRPYIDSGGEPFFLDRYEGKLFVARLDEAG
                     NWCWAARTEASQPQHLIGADIDFKGNLRVTGSCPGEMSFGAQQP"
BASE COUNT         1370 a         1554 c         1642 g         1200 t
ORIGIN      
        1 tgtaagatct acacccacat gcacctggcg caagatgacc tgcgcctctt cggtttcgcc
       61 tctcctgcgg aacgcgagct ttaccagcaa ctgaaccgca tttccggagt ggggcccaaa
      121 agcgctttgt caatcatctc caccctaccc atacccacat tcgtgaaagc catcgagcgc
      181 gaggaaagcg ccctgctcac aaaggtgccc ggcattggca tgaaaagcgc ccagcggctt
      241 atcatcgagc taaagggcaa gttgcgccat ctgatggatt acgctgaacc cggccagttg
      301 ggagtggagg aagacaaggt cactgaggtg gaaaatgccc tgcagaccct gggcttcagc
      361 ccaaaggacg ttcgccgcga actgagtctg atgggcgagg aagcccaggc tcaagccacc
      421 gagcagctga tcaaggaagt gatccgccgc ctttaccaga gaagtcgtta aatggtacgt
      481 aaagaagcct atcagaccat cctgagaatt ttcaaggaca aggagttttc agacaccctg
      541 ttgcatcagc gggcaaagcg attgaagagc gaccgcgtta acgtggccct cttttacaac
      601 atggtgaaag gcgtcgtcaa gcttaggggc aaacttgact atatcctgtc ccagcatgcg
      661 gacgaggcca aattcgccgc caccgacata aagatcaaga tctggctcta cctgggtctc
      721 taccaactta tatatctgga ttccattccc gcccacgcgg ctatcaacga aacggtgaaa
      781 ctggccaagg aaaacctggg cgacaaggtg ggcgatttcg tgaacgccat gctgcgttcc
      841 tggcagcgcg agccggaagt ggtttacccg actgaccccg tccaacgaat cgcctgcgaa
      901 cactcctatc caccagaact tatcgaacag tggatagacc tctggggcga ggaagatgcc
      961 gaatatctgg ccatctggtt caacgagaat cccaaactgc atatgcgggt caacggaacg
     1021 gccaccaccc cggagaaact catccagtac tttgccaagc gcgacatcaa ggtgacgcct
     1081 tccccggtgg ggaaaatgac cctgctcacg gaggaagcgg actccgtttt ggacgatgtg
     1141 gctttttcag aaggctattt ttccatccag gacacctcct cctcgctgat agtggaacta
     1201 ctggaccccc agccggagga aaacatcctg gacctcttcg cggcgcccgg cggaaaatgc
     1261 tcctacatcg ccgaaatcct gaacaacaca ggcgaagtgg tggctgtgga caagattccc
     1321 agcaagatga agctactcaa gcaggcggcc gacagactgc aactgagcaa cataaaaccg
     1381 attgtttcag acgcttttaa atatggtcca gtagctccgg cttatgacag ggtgctggtg
     1441 gatgccccct gttccggctg gggcgttttc gggcgcaaag ccgacctccg ctggcaggcg
     1501 catcagaaca tcgaagaact ggtaaagctg caggaaaagg ctttggcgct cggagccaat
     1561 tttgtacgcc cggaagggat catggtttat tccacctgca cgatgaaccc cgaagagaat
     1621 gagcgccaga tagagaagtt ccttgcccgg aacccgcgtt tcacattgat tgacgccagt
     1681 gaaaagcttc ccggggattt ttgccagggt gggatgttcc gtagcctgcc tttccgccac
     1741 aacatggacg gggcttttgc cgccaaaatg atcaaaacga ggtaatgcca agtggccagc
     1801 gaaataagca aaaagttttg gctcacggtt gggatcggcg ccgggataat ctttctctcc
     1861 gccttcatca ccagccagat cattttcccc atcatcttcc gcgagcccaa gcagatcgag
     1921 gtgccgaacc tcgtgggcat aagttccgcg gctgccaggc agaccctgag cgcgcttggg
     1981 ctgcatgccg ttgtgaagga ctccatctgg tctgagactg agcgcatcga caccgttttg
     2041 gaacagaacc ccgcgggcgg agcgctgata aaaccggaag gcacagttta cctgcgcgtg
     2101 agccggggaa cccgaaaggt cggggtgccg tcggtggttg gcctcagcta ccaggaagct
     2161 tactacaccc tcgttaacgc aggcctgaag ggtgttgtgg cggattccct ctattcagac
     2221 agctacgcgc caaacacggt tatccgctgc attccggggg tgggatcaaa aatcgagctg
     2281 ggcgcggtgg tccgaatgta catgagccgg ggtccggaac ccgttatcca gggaggagca
     2341 tttgccgaca ccacgctgca atatgaaact cctttcgaaa actggtaacg cccgtgaccg
     2401 ctgatttgaa cgcttcgggg accctgcggc tggatattcc cctggataag cagcgattgt
     2461 tcgggtttct gttggattca gtggaaggcc tggccacaca tagccgggcg caggattctg
     2521 aacagcttga agtctggata accgcggggc aggagcaggc ttttcaggat ttcatgaggg
     2581 cttgggacgc atttcagtcc cgaagccgcg agcaaacacc tctttgatca ggctggaata
     2641 gcggtcagat cagccccgtt gaacgcatca gggtttcgcc cgccaaggcc cagatcacag
     2701 ccgccagggc gatgaagttg ccgaaagcga aagcttgttc cggcttgcgg atgaagagca
     2761 ggaaatagag aatccccatc agacaggcga gcaggatcac aaaggggatg tgaattacgc
     2821 cgaaaaaggc cgccactgcc ggaatcagga tcacatcgcc gccacccagt ccctctttgt
     2881 ggaaaaactt ctcccagccc agcgccagca gatagagcag cgcgaacagc cccagagctg
     2941 gcagcaggta aacccaaaag aagtccacga agttaaaccc gctctggatg aaactcagca
     3001 gcattcccac agccaccatt ggcaacgaca gcaccagcgg tatgatcttg tgcatggcgt
     3061 cgatgaaaaa gatggggatc aggaatccga acatcacggc gaatttccaa aaactgaagc
     3121 ttgcgatccc gtaaacgaag gacagcgcca aaaaaagcag cggtgtggca atttccacag
     3181 caaggtggtg ccagtggatt ttcgcgccgc agaatttgca tttgcctttc tgggccagat
     3241 aggtgtagat ggggatgttt tgccgggcgg gaagctgttt gccgcaggat gtgcagtgcg
     3301 aggggggatt cacaaccgat tccctgcggg ggatgcggtc gatgaggacg ttaaaaaagc
     3361 tgcccaggac ggcgcccagg acagctatga tgattgagac aatcagcgtc aaaaggcatt
     3421 accacctttc ataccggggc cgctggtcac tgtaccagtt tgacggtccc gtgtaccaga
     3481 gtgttccctg cccgaattcc acggagaggt tcacgctgtc cgaggggcgg tagttgaggc
     3541 ggaactcggg gataaccttt gtgttgttgt cggaattgaa ttgcagtttg gaggtggaac
     3601 cgtagttcac caggctgagg tcaagctcaa ggtccagttt gggattgaag gcgtagttga
     3661 tgtggttggt gtagcgggag aggtaatatc cgcgcccgct gctgctggtg ccggcttcaa
     3721 agcccaggga atggctcatg gaaagtttgt tcagcccaaa gagcggggtg ccgcttccct
     3781 tgtagggatt aaggttcggt ttgggcatat ctatctgtgc cagcaagggc aaactgagca
     3841 gcaggatcag gattccgatc agggtctttt tcattcggat actccttgtt tattcttctt
     3901 cgcaacaaaa taactcacac cccaatatct gtcaagtgtc ttgaaaagcg gctttctttc
     3961 aagcggatat tagcccggag ggcgacagaa caccctcata ttctgtcgcc cgcaaggggc
     4021 ttttgttttt tcgggctccg atcccgaggg ggcttacgca ccctcgctat cgttctgtcg
     4081 cctctgcgag gcatcactgg ctgaatcccc gagataaaaa tgacaaggtg gaattatcac
     4141 tattgttcaa ttactcaccg ggcttgtaat catcatatag ccctgccagc caggccctga
     4201 gcgctcgcgt catttcccct ctcactcctc tcgtaagatt cccgtatcaa atacggccat
     4261 tatgcgggag gcgagaatgg ggaacggcaa aaggccttaa ggaccagcca acatcgcgtc
     4321 ggtaagatat gctgaattgg gagagatgga aggcggcaac ggcagatttg gagcggagat
     4381 tggggttgcg gaaactttcc gcggcgcatt gggtttttcc ttgggggaaa accgcctatt
     4441 atatctttat aataaataga gaggaccgac aagaatgaaa cagaccgacc tgacccaagc
     4501 ctatggcctg gtccaccttc ccgccaaggt ggccctcgcg gtcaccgaaa accccggcgg
     4561 aggctataac ctgattacac tggagtggtt catgcgcacc tccatccagc ctcctatgtt
     4621 cgccatttcg gttggccaca cccggttttc gtatgaatgt ctgcaggaaa acaggttttt
     4681 caacctggtc ttcccctcgg cggagatgaa gccgctatgc gccctggccg gctccacttc
     4741 gggccgggac accgacaagt tcaagaaggg cggggtggaa tccatccccg gaaaataccg
     4801 caggctgcct gtccccagag aggcggtggc ggtgtttgaa tgtgaggtca catcccaggt
     4861 gagaagcggt gaccacacca tctttgtggg tgaagtgcgc caatgctggg caaatgagag
     4921 taaagagctg ctcgtctatt caggaaagga gctgtaagga gtcgggagtc gcggtatcag
     4981 ggctgttggg cgccgaagct catttctcca gggcaggaac ccgtaacccg caggtttcct
     5041 ttgaaatcga tgtccgcgcc aatcaggtgc tggggctggc tggcttccgt ccgggccgcc
     5101 caacaccagt ttccggcttc atccaggcgg gctacgaaaa gcttcccttc atatcggtca
     5161 aggaagaaag gttcgccacc ggaatcgatg taaggcctgt atttggccac cagatataga
     5221 ttcccggcca ggtcattatc cagaaagagg aggtctgttc ccaaccattt gcgggcccag
     5281 aggcatttgc cgtcggcgtc gaaacgcgcg taaaactggg ctttatgtcc tccggcgctt
     5341 aacttctggc tgcccaggct gaggctgccg gaaaaagttc ctgccacaaa caggtccttc
     5401 tggcggcctg agcttatcca atcgatggaa acgtccgttc cttccagaga atgggtccag
     5461 agctttctac cctggctgtc caccaaagaa aggcttgaat tcacgctcaa agcgccatcg
     5521 tagatggggt tggggatggg gtttccgtaa taattgtccg cgaaccaggt attgccagcg
     5581 gcatcggtgt cgaaacactt tctgaagcaa tcggtggaaa gctgctgcag gcggctgccc
     5641 cagttccaag ttccgtcggc gccaagccgg gagatgaaaa tgacgttacc ggcgccatcg
     5701 ggcttggagc cgatgttagc gccacccagc acaaggttgc cggtgctgga gccggcgatg
     5761 attaaa
//
LOCUS       DAOOWR010000074         5764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14086_length_5764_cov_4.421615, whole genome shotgun sequence.
ACCESSION   DAOOWR010000074 DAOOWR010000000
VERSION     DAOOWR010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14086_length_5764_cov_4.421615"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLB85_02845"
     CDS             <1..730
                     /locus_tag="PLB85_02845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOU72294.1"
                     /translation="LKLLFQTMIGVVMYAIGFRVVSLTNPFGAEIVLYWASFPVTLLW
                     YLVVMNAVNLIDGIDGLASGICVIVCAVLLSVGVKEHNLLVTALSAFLLAGNLAFLRF
                     NFHPAKIFLGDTGSLFNGLVIAAISTAGTQQYKGITSMTLIIPLSVLAIPLIDTALAV
                     FRRLRLGNIFIADKAHLHHAMLGLGLSQKAIALTAYVVTLLFGLIAIGFSFSTKRILF
                     SVLFGLLVLGVILAYVLMRHGRKK"
     gene            727..3204
                     /locus_tag="PLB85_02850"
     CDS             727..3204
                     /locus_tag="PLB85_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72295.1"
                     /translation="MKRILLLALLAPLCLTLSATNWQTITNLSHVYDFVPLYEYDTYF
                     ATWGGVVHSWGGNGEIPAGQEILTTADGLASNDVRTLEYIAFNQSLWLGTAASGITII
                     MPQGVQQLNTSLGLLSNNVVKIVEHGANILVATKAGLASYYYLEGVNFPLMLHQYTTQ
                     TTPGLLSNSIEAMELGSTNYLYISSADGLNFVHLDSLDVDSAWHSFGDYPGGGSIRGK
                     LSVRPGHLTVAGQLAVYTRSDDPWTGTWKTYSNSDGLILEQISSAALDGRGALWVSYG
                     SWNEDFLSYSRNTEVLLTRIDSNGSVHHWTEAQNYLGTKSICRIVTYEDMVYLCSWGN
                     GVFSQREGVFPESLVWHQTLPNSIGFPKITNIATDANHAAWFCSGGLSATPIRKSTLG
                     ASKYQDGVWQTFNISNSPIHTDNIYCCAVDSHNRKWFGTYDVNDTSPEGWRYGVSIFD
                     EASGTWKFLDRDGIHIWDPELGSYGGAIPGSARLLGNTDLHISQDRHGNMFVACYNDG
                     FSVLDPDDNLIAEFIIPNSAHQRSIYCYHSGSQYFIGTYNDRGLVIWNHDSIPETDGP
                     HWLIPDPPDLNNCEVYGIVSVNSPYEGIQHWIAASSGLFMWDEEDWYKWDTSIKRFKF
                     NKKSRAWENEILYYVDEERLYGSVRTTPTAIYLDPFNRVWIGSLEHGISMYDPETERF
                     TNYFQKNSPLLSNYITALGYNPVEGILLIGTPDGLNTFKIGYSEKPEVRLENLVIYPN
                     PFRPAERGSVIIRNDPTETMPRGLNQCRIYDSAGTLMRILEENKFARFEWNGANAEGK
                     ACANGIYFVVVSDAKGNRRTGKIALLR"
     gene            complement(3600..4703)
                     /locus_tag="PLB85_02855"
     CDS             complement(3600..4703)
                     /locus_tag="PLB85_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOU72296.1"
                     /translation="MSFNLAAIRRELHRTPELAFREEKTKLLILSWLRQISGIRIHEF
                     STNNGILVEYSRGEGPYRLFRADMDALPVSESTGCPFSSQNPGLMHACGHDVHMTVLL
                     GLIERMAEQNVRRNLLFLFQPAEEGEGGAQSVIAEGLIQRFDVEAAFALHVASGLPVG
                     TVSSRPGVFFGVPQEFDVQFFGKTAHAAFPAKGINALDAGRNFLSLMEKDVSDLAKQH
                     RVIFHVGKATAGTIRNVVPNHCKLEGTHRSLDKEVSLRINELISNNASLTTKMYGAEY
                     RVDFLVTYDPVVNSADLVEELKRACAETGTKYQKAEASLTGEDFGFFTSLYPGLLFWL
                     GSGCAEPLHSDKFLPDEACLATGVDIFAALARR"
     gene            complement(4700..5680)
                     /locus_tag="PLB85_02860"
     CDS             complement(4700..5680)
                     /locus_tag="PLB85_02860"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU72297.1"
                     /translation="MKLAVVGATGEVGRMMITCLEEFAVPVESFTLLASARSAGSTLY
                     YADTALSVRELTSAEMREPFDYVLFSAGAGVAREFAPLAAASGAVVIDNSSGFRQEKD
                     VPLVVPEINGNLLKGYQGIVANPNCTTIQMVLPLAVLDRLFGLRKVVVSTYQSVSGSG
                     HKGVATLESQRAGGSENGIYPNLIDLNVIPQIGSFADNGYSQEEEKIHFETRKILNNW
                     NLEVSATTVRVPVIYGHCESIYAEFASEVDLAVAARELGKAESIAFEENTYVTPRDLG
                     NSNDSHVCRLRRGTGPNSLCFWNVGHNVRLGAAANAVRILLRHAQLAGRI"
BASE COUNT         1273 a         1638 c         1580 g         1273 t
ORIGIN      
        1 actgaaacta ttattccaga cgatgatcgg cgtggtgatg tacgccattg gcttccgcgt
       61 ggtttctctt accaatcctt ttggcgcgga aatcgtttta tactgggctt ctttccccgt
      121 caccctgctc tggtatctgg tggtgatgaa cgctgtgaac ctcattgatg gcatcgacgg
      181 cctggccagc gggatctgcg tgatcgtttg cgctgtgctg ctgagcgtgg gcgtgaagga
      241 acacaacctt ttggtcacag cgctctcagc ctttctgctg gcgggaaacc tggctttcct
      301 gcgcttcaat ttccatcccg ccaagatctt tctgggcgat accggctcgc tgttcaacgg
      361 cctcgtgatt gccgctatat ccaccgccgg gacccagcaa tacaaaggca tcacctcaat
      421 gacgctgatc attccgctgt ctgtgctggc gatacctctg atcgacaccg ccttggctgt
      481 tttccgccgt ctgcgcctgg gcaacatttt catagccgac aaagcccacc tgcatcatgc
      541 catgctggga ctgggccttt cccaaaaagc catcgcgctc accgcttatg ttgtcaccct
      601 gctctttggc ctcatcgcca tcggcttttc cttttccacc aagcgcatcc tcttctcggt
      661 actgttcggt ttgctggtat tgggtgtgat cttggcctac gtccttatgc ggcatgggag
      721 aaaaaaatga aacgaatcct tttactggcg ctgctcgcgc ctctttgcct gacgctgagt
      781 gccaccaact ggcaaaccat caccaacctc agccatgttt acgattttgt tccgttatat
      841 gaatatgaca cctattttgc cacctggggc ggagtagtcc attcctgggg tgggaacgga
      901 gaaatccccg cggggcagga aatcctaacc accgcggacg gactggcctc caatgacgtg
      961 cgcaccctcg aatacattgc ctttaaccag agcctctggc tgggaaccgc cgccagcggc
     1021 atcacgatca tcatgcccca gggtgtccaa caactgaaca cctctctggg cctgctttcc
     1081 aacaacgtgg ttaagatcgt ggaacacggc gccaacatcc tggtggccac caaagccggc
     1141 ctggcttcct attactatct ggaaggtgtg aattttcccc tgatgctgca ccaatacacc
     1201 acgcaaacca cgccgggctt gctttccaac tccatcgagg ccatggagtt gggctccacc
     1261 aactatctct acatcagcag cgcggatggg ttgaatttcg tgcatctgga ttccttggac
     1321 gtggattccg cctggcacag ttttggcgat tatccaggcg gcggcagcat caggggcaaa
     1381 ctgtccgttc ggccgggaca tctcaccgtg gcaggccaat tggcagttta cacgcggtcc
     1441 gatgacccct ggacaggcac ctggaaaacc tacagcaatt ctgatggatt gatcctggaa
     1501 cagatatcca gcgccgcttt ggacggccgg ggtgccctgt gggtgtccta tggcagttgg
     1561 aacgaggatt tcctcagcta ctccagaaac accgaggtcc tgctcacccg gatcgacagc
     1621 aacggcagcg tgcaccactg gactgaagca cagaattatt tgggcaccaa aagcatctgc
     1681 aggatcgtca cctacgaaga catggtctat ctctgcagct gggggaacgg cgttttttcg
     1741 cagcgggaag gcgttttccc cgaatctctt gtctggcatc agacgctgcc caattccatc
     1801 ggttttccaa aaatcaccaa catcgccacc gatgctaacc atgccgcctg gttctgcagt
     1861 ggcgggttaa gcgccacacc catccgtaaa agcaccctgg gcgccagcaa atatcaggat
     1921 ggcgtctggc aaacattcaa catttccaac agtcccatcc acacggacaa catctattgc
     1981 tgcgctgtgg acagccacaa ccgcaaatgg tttggcacct acgacgtgaa cgacaccagc
     2041 cctgaaggtt ggagatacgg cgtttccatc tttgacgagg caagcggaac ctggaaattt
     2101 ctggaccgcg acggaatcca tatttgggac ccggaactcg gcagttacgg cggtgccatc
     2161 cccggttcgg cgcgcctgct tggcaacacg gacctgcata tatcccagga ccggcacggc
     2221 aacatgttcg tggcttgcta taacgacggc ttcagcgtgc tggacccgga tgacaacctc
     2281 atcgccgagt ttatcatccc gaacagcgcc catcaacgct ccatctactg ctatcatagt
     2341 ggcagccaat actttatcgg cacctacaac gaccgggggc tggtgatctg gaaccatgat
     2401 tccatccccg agaccgatgg cccgcactgg cttatccccg atccgccgga tttaaacaat
     2461 tgcgaagttt acggcattgt aagcgttaac agcccttacg agggcatcca gcactggatc
     2521 gcggccagct caggtctgtt catgtgggac gaggaagact ggtacaaatg ggacacctcg
     2581 atcaaacgtt tcaaattcaa taaaaagagc cgcgcctggg aaaacgaaat cctctactac
     2641 gtagatgagg aaaggcttta tggctcggtg cgcacaactc ccaccgccat ttaccttgat
     2701 cccttcaacc gcgtctggat cggcagcctc gagcacggca tcagcatgta cgatccggaa
     2761 acagaacgct tcaccaacta tttccagaaa aactcgcccc tgctttcgaa ctacatcacc
     2821 gccctcggct acaaccccgt ggaaggaatc ctgctgatcg gcaccccgga tggcctgaac
     2881 actttcaaaa tcggctattc cgagaaaccg gaagtcaggc tggaaaacct ggtgatctat
     2941 cccaatccct tccgccccgc cgagcgcggc tctgtgatca tacgcaacga tcccacggaa
     3001 accatgcccc gcggcctcaa ccagtgccgt atctatgatt ctgccggaac cctgatgcgc
     3061 atcctggagg aaaacaaatt cgcccgcttt gagtggaacg gcgccaacgc cgaaggcaaa
     3121 gcctgcgcga acggcatata tttcgtggtg gttagcgacg ccaaaggcaa ccggaggacg
     3181 ggtaagatcg cgctgctgag gtgagttatc acgatgtgaa ccacgagcct ggatctgcgt
     3241 aaaacgacaa tgatttcagg ctctttttct aaccgggagc ctggcgtaca gcgaacgcag
     3301 tgagcatgga cgacaggaga atacgtagaa gcgctcttcc tttgctgaca tcgatgctgt
     3361 gcccgacgcc agcttttatc ctggcgaagc tgactggccg ttaaggcctt ctataggatt
     3421 ccggattcgt ctcccgcatg atacccgaat ttgatgcgag aattatgcgg gaggcatgag
     3481 agagggatgg aatcgggcgc taagggtgag acaggcagga catcacggtg ggtaaacagt
     3541 tatggctccc tttcaagtct ggtggattac caacttggat tgatagagag ccaaatgttt
     3601 tagcggcggg ctaaagcggc aaaaatatca acgccggtgg cgaggcaggc ttcgtcgggt
     3661 aaaaacttgt cggaatgcaa aggttccgcg cagccggagc ccagccagaa gagcaagccg
     3721 gggtagaggc tggtgaagaa accgaaatcc tcgccggtga gcgacgcttc agctttctgg
     3781 tacttcgttc cggtttccgc gcaggcccgt ttcaactctt ctaccaaatc ggcggagttg
     3841 accactggat cgtaagtgac cagaaaatca acccgatact cagcgccata catctttgtg
     3901 gttagggaag cgttgttgct gatgagttcg tttattcgca ggctcacttc cttgtccagg
     3961 ctgcgatgcg tgccttccag tttacaatgg ttcggcacca cgttgcgaat tgttccggct
     4021 gtagcttttc ccacatggaa gatcacccgg tgctgtttgg ccaggtcgga gacgtctttc
     4081 tccatcaggc tcaggaagtt gcggccggcg tccagggcgt tgatcccttt tgcgggaaag
     4141 gcagcgtggg cggttttgcc aaagaactga acatcgaatt cctggggcac gccgaagaac
     4201 acgccagggc ggctggagac cgttcccacc ggcaatccgc tggcaacgtg cagcgcgaag
     4261 gcggcctcca cgtcgaaccg ctggatcagg ccttcggcga tcacgctctg cgcgccgcct
     4321 tcaccctctt cagccggctg aaagagaaag agcagattgc gccggacgtt ttgttcagcc
     4381 atccgctcga tcaggcccag cagcaccgtc atgtggacat cgtggccgca ggcatgcatc
     4441 agtccggggt tttgggagga aaacgggcag ccggtgcttt cagagaccgg cagggcatcc
     4501 atgtccgcgc gaaacagcct gtaagggcct tcgccacggc tgtattccac cagaatgccg
     4561 ttattggttg aaaattcgtg gattctgatg ccagaaatct gcctcaacca agacaggatc
     4621 aagagtttgg tcttttcttc ccggaaagcc agttctggag tccggtgcag ttcacggcgg
     4681 atggcggcaa ggttgaagct catatcctgc ctgccagttg ggcgtgtctg agcaagatgc
     4741 gaacggcgtt ggcggcggcg ccgagacgga cgttgtggcc cacattccaa aagcagaggg
     4801 agtttgggcc ggtgccgcgg cgcagacggc agacgtggct gtcgttagag ttacccagat
     4861 cgcgcggggt gacataggtg ttttcttcaa aggcgatgga ctctgctttt cccaattccc
     4921 tggcggccac ggcgagatcg acctcagaag cgaattccgc gtagatgctt tcgcagtgac
     4981 catagatcac gggtacgcga acggtggtgg cgctgacttc caggttccag ttgtttagta
     5041 tcttgcgggt ttcaaaatgg atcttttcct cttcctggga gtagccattg tcggcaaaag
     5101 agccgatctg ggggatcaca ttgaggtcga tgaggttagg gtagatgccg ttttctgatc
     5161 caccggcgcg ctgtgactcg agggtggcaa cgcccttgtg gccgcttccg gatacggact
     5221 ggtaggtgct aacgacaact ttgcgcaatc cgaagagccg gtccagcact gccaaaggca
     5281 gcaccatctg gatggtggtg cagtttggat tggcgacgat gccctgatac cctttcaaaa
     5341 gatttccgtt gatttccggc acaaccagcg ggacatcctt ttcctggcga aacccggagg
     5401 agttgtcgat caccacggca ccgcttgccg ccgcgagagg cgcgaattcg cgggctacgc
     5461 ctgctccggc ggaaaagaga acgtaatcaa agggttcgcg catttcggct gaagtgagtt
     5521 cacgcacgga gagagccgta tcggcgtagt agagagtgct tccggcagaa cgagcggagg
     5581 cgagcaaggt gaaggattcc accggcacag cgaattcctc cagacaggtg atcatcatgc
     5641 ggcccacttc tccggtggcg ccaacaacag ctagtttcat gctaagtcct caatcgcgat
     5701 aaatctgaaa aatccttttc cgcagaggcc gggcttttgt caatgcaaaa cttgacaaaa
     5761 tcag
//
LOCUS       DAOOWR010000075         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14112_length_5757_cov_3.754121, whole genome shotgun sequence.
ACCESSION   DAOOWR010000075 DAOOWR010000000
VERSION     DAOOWR010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14112_length_5757_cov_3.754121"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1021..2553)
                     /locus_tag="PLB85_02865"
     CDS             complement(1021..2553)
                     /locus_tag="PLB85_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU72298.1"
                     /translation="MLNFSQAPDGTKYPIPTEEDNARERELLVKITEEQRALGRKIVA
                     VQGLGFVGCVMATVVADATDKDGNPYYFVHGHQRASKRSFWKVPVINSGVPPVSSSDP
                     EVPQIFHRTVVEKKNFRATSEDSVYSLVDIVVVDIQLDATKPSFGEAEKGYCDLFAFR
                     EGIRTLGQHIRPDCLVLVETTVPPGTCEKVVKPILEEEFTKRGIDIVANPPLVAHSYE
                     RVMPGAKYVASIRDFWRVFSGVNQKSIELCHEFLSNVLNVEEFPLTQLDNTNASELAK
                     TMENTYRAVNIALTLEWARFAEQIGVDIFKVRDAIRKRKGTHDNMLRPSLGVGGYCLT
                     KDPVLANWAMQTLFGLEGTLEMAIRGVNINDTMPLHTIEIIKTVIPELRDVNVAVLGV
                     SYLENVGDTRHSPSKTLVEFLRKEFAKVLTHDPYVEAWPELDESKVESDLKAVLPGAD
                     VVVFAVGHDQYKDLSPAEVLAICGTKPLIVDCSNFLSDEKIAEYKRLGCQVRGVGKGH
                     IV"
     gene            complement(2684..4075)
                     /locus_tag="PLB85_02870"
     CDS             complement(2684..4075)
                     /locus_tag="PLB85_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU72299.1"
                     /translation="MKKKNIQVLEEMPIARAILHLALPSVLSMMVNILYNLTDTFFIG
                     KLHNEALVAAVSISLPLFTLQMAVAGIFGVGGSSYLSRLLGRKDFRKARETTTTAVFS
                     SFVFSIVLGVVGILCIPLFLKVTGASGETAVAARQYMFWILLGSPFVMLKFTMVQLVR
                     GEGGARQAMYGLVIGTVTNIILDPIFIFGFKMGVTGAAIATVIGQGLAMCYFIRYYLS
                     KQAVAAPALRFLRLRWEIYREILLIGVPSSLSQVMMGIGNTISYNLASAYGVSAVAAL
                     GVASRVFSIPIFVFIGVSIGVQALIGFNYGAGNYPRMKKAISTAVMINLSLSVVFTLL
                     FVLFPRQLISMITPIKQTVEIGRQVIGAYLYAIPFAGVGMIMMNSLQAMGKALPAFIV
                     SISRQGLAYIPALLLLNRFFGFDGLIFAMPLSDFVTTLLSGIFVISILSRLKHHPAPS
                     FQEFSQPQPADEI"
     gene            4332..5519
                     /locus_tag="PLB85_02875"
     CDS             4332..5519
                     /locus_tag="PLB85_02875"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72300.1"
                     /translation="MRSKVFCLLLVMAAMATLVAGELPATSCGERDAGTITGVVRNTF
                     NQPIQGAAVSCGTVTAATNASGMYSMQIEAGTYSVTASHPDHQSATQAGVVVVGGQTI
                     YCNFILHFDPFPFGFYDSFEDHEDFALEFAPWTCVDVDLSETSGISGVNWPHAGEPQA
                     FIIFNPTATIPPLSGIQAYSGDKMAASFPATQSPSSDWLITPAVKGMDFCQFRARSLN
                     ADNGLERIKVGVSTFGTVPQSFVIISGAQALEVPASWTQYIFDLSGFEDEIVYVGIHH
                     CTDGGSALLVDSFNAENVPVADDSQTPALTGIEGNFPNPFNPETNIRFSLARSGPVEL
                     GVFDLRGRKLKTLVNSYLPSGHHTTVWNGLDDAGRQVSSGVYLCKLRAGGQRSCHKLV
                     LMK"
BASE COUNT         1318 a         1522 c         1599 g         1318 t
ORIGIN      
        1 taagccattc tccctacttt ctccctactg gattgatttg tgaatttata caaaagggag
       61 gggttttccc ctccctttga caggtcattt ttggacccat gattccatga taaggtcatt
      121 taacacatac acttttccag ttttgccatt tttcagtctc acggcttcca cggtcaacgc
      181 ctccctttct atcaaggact ccgtcacttc gcggaactcc ctctttttca tatgggaagc
      241 attcatcaac tgggaatggc ttgccctgcc attgtattta ttccggaatg acgtggtggg
      301 ctggcgtaga gcgaggaacg agcatcgacg acagcaatga atgactcttc caatgtttcc
      361 tgccgttcat tccgttgaac ttcctgccgt ccatgctcac tgtgttcgct gtacgccagg
      421 ctccaccagc atggacgcca ggggggtctg gattccgggt caagcccgga atgacgtggg
      481 tgtggggctg ggtggtggtt tataggctat accgcccggg ctggatttcg ggtcaagccc
      541 ggaatgatgt ggtggggccc gcgcttatat gtgggccatt tttctatcca cgaattaaga
      601 cctttgcaca taagtcacgg aagttaaatc cagaccatga agcgaatgcc cccgacgctc
      661 aattgcaata atgaacagtt cttggggaac actcggacta caatggattg gaagatttcc
      721 cagctattaa taaaaatact ggaaattctt gaacggggag acagatacaa ccactttatg
      781 acagatgcac ggcgcccgtc tggagcatca gatggaaaac tatccgtcat gatctgatca
      841 acacacccaa ccattgcatc cttatccagt cctaagttgc gccagatcag gaaattgctt
      901 tgggttggat ggcgtctgac tggcagggtg aatgaacgcc gctaaaagat aggctggaaa
      961 aaatccccac gaaaaaaggc gggagctggt cccgccttgt atgtatccaa ttattggaga
     1021 ttagacgatg tggccttttc ccacgccgcg gacctggcat ccaagccgtt tatactcagc
     1081 tattttctcg tcggagagga agttcgagca gtccacgatc aggggtttgg taccgcagat
     1141 ggccagcact tcggccgggg agaggtcctt atactggtcg tgccccacgg cgaagaccac
     1201 cacgtcggca ccgggcagaa ctgctttgag gtcactttcc acctttgatt cgtcgagttc
     1261 cggccaggct tccacatagg gatcgtgggt gagcactttg gcaaattcct tgcgcaggaa
     1321 ctccaccagg gtcttggagg gtgagtgtct ggtgtcgcca acgttttcca gataggaaac
     1381 acccaggacg gcgacgttca catcacgcaa ttccgggatc acggtcttga taatctcgat
     1441 ggtgtggagc ggcatggtgt cgttgatgtt cacgccacgg atggccattt ccaaagttcc
     1501 ctcgaggccg aagagggttt gcatggccca gttggcgagc acggggtctt tggtgaggca
     1561 gtagccgccg acgcccagac tggggcgcaa catattgtca tgagtgcctt tgcgcttccg
     1621 gatcgcatcg cgcaccttga agatgtcaac gccgatctgt tcggcgaagc gggcccattc
     1681 cagggtgagg gcgatgttca cagcgcggta ggtgttttcc atcgttttgg ccagttcgga
     1741 ggcgttggtg ttgtcaagct gggtgagggg aaattcctcc acgttcagca cgttggacaa
     1801 gaattcgtgg cagagctcga tgctcttttg gttcacaccg gaaaagacgc gccagaaatc
     1861 gcggatggag gcgacgtatt tggctccggg catcacgcgc tcgtaagaat gcgccacgag
     1921 gggcggattg gcgacaatat cgatgccgcg tttggtgaat tcctcttcca gaatgggctt
     1981 caccactttt tcgcaggttc caggcggaac ggtggtttcc accagcacca ggcagtcggg
     2041 gcggatgtgc tggcccagcg tgcggattcc ttcgcggaag gcaaagaggt cgcaataacc
     2101 tttttccgcc tcgccgaaag agggtttggt ggcatcaagc tggatgtcca cgaccacgat
     2161 gtcaacgagg ctgtaaacag aatcttcgct ggtggcgcgg aagttcttct tttccaccac
     2221 ggtgcggtgg aaaatttggg gaacctcagg atcggatgaa ctgaccggcg gcactccgga
     2281 attgataaca ggcactttcc agaacgagcg tttggaggca cgctgatgcc catggacaaa
     2341 gtaataagga ttgccgtcct tgtcggtggc gtcggccacc acggtagcca tcacgcagcc
     2401 cacaaagccc agaccttgta cggccacgat cttacggccc agcgcgcgct gctcctcggt
     2461 aattttgact aacaactcgc gttcgcgagc gttatcttct tcagtgggga ttggatactt
     2521 ggtgccgtct ggcgcttgtg aaaagttaag catatgtgaa gttctccttt attcgattat
     2581 cgcattggcg ttcaaaaaaa acaggggcaa agcatgtcaa ggcttttcgt taagggcatg
     2641 gctgaattct ctccggaagc taaatccaga catgccggac aaatcagatc tcatccgctg
     2701 gctgtggttg gctgaattcc tggaaggacg gtgcgggatg gtgttttagc cggctgagga
     2761 tcgagatcac gaagattccc gaaagcaggg tggtgacgaa gtccgacagc ggcatcgcga
     2821 agatcaggcc gtcaaagccg aagaatctgt tcagaagcag cagcgccgga atgtaggcca
     2881 ggccctggcg cgagatggaa acgatgaaag caggcagggc cttgcccatt gcctgcaggc
     2941 tattcatcat gatcatgcca accccggcga agggaatggc gtaaagatag gccccgatta
     3001 cctggcggcc gatttccacg gtctgtttga tgggggtgat catgctgatc aactgccggg
     3061 ggaagaggac aaaaagtagg gtgaaaacca cgctgagact tagattgatc atcacggcgg
     3121 tgctgatggc ctttttcatg cggggatagt ttccggcgcc atagttgaag ccgataagcg
     3181 cctgcacgcc gatggaaacc ccgataaaca cgaaaatggg gatggaaaac acccgcgaag
     3241 ccactcccag agcggctacg gcgctcactc cgtaggcaga ggcaaggttg taagatatgg
     3301 tgttgccgat gcccatcatc acctggctga gtgaggacgg gacgccgatt agcaaaatct
     3361 cgcggtaaat ctcccagcgc aggcgcagga acctcagagc cggtgcagca accgcttgtt
     3421 tggaaagata atacctgatg aaatagcaca tggccagacc ctgcccgatc accgtggcaa
     3481 tggccgcgcc ggtgaccccc attttaaatc caaagataaa aatgggatct aagattatat
     3541 tggtgacagt accgattacc agaccataca tggcctggcg ggcgccgcct tcgccgcgga
     3601 ccaactgcac catggtgaat ttcagcatca caaagggact tccgagcaga atccaaaaca
     3661 tatactgcct ggctgccact gccgtttcgc cgctggcgcc ggtgaccttc aaaaacagag
     3721 ggatgcaaag aatgcccacc actcccaaaa caatggaaaa cacaaatgag ctgaataccg
     3781 cagtggtagt tgtttcccgg gccttccgga agtcttttct gcccagaagc cgggagaggt
     3841 agctggagcc accaaccccg aatatccccg cgaccgccat ctgcaaggtg aagagcggca
     3901 aagaaatgga caccgccgcc accagggctt cattgtgcag cttgccgatg aaaaaggtgt
     3961 ccgtgaggtt gtataaaata ttcaccatca tgctcagcac gctgggcagg gccagatgca
     4021 gtatcgcccg ggcgatcggc atttcctcca aaacctggat gttctttttc ttcattatct
     4081 cttatcaaat tccttcgcat gcgaagtttt tcagcagggg gattctgtca acctccgcgg
     4141 aattagcggg ttgaaaaacg ggtgtgagaa ccgttcagca ggggaatttg ccttcgctaa
     4201 agataatccg agtttttatc tcggcaataa aacattactt gacacatatc ataaggatgg
     4261 aataaatgta gccatgaggt tgtggtgatg cgagcggcat cccctgccca aaaagaaaag
     4321 gaggcaagat catgcgctcc aaggtgtttt gtctgctgct ggtgatggct gccatggcca
     4381 cgctggtcgc tggagaatta ccggcaacat catgtgggga aagggatgcg gggaccatta
     4441 caggcgttgt gcgcaatact tttaaccaac ccatccaggg ggccgccgta tcctgcggta
     4501 ccgtgaccgc cgccaccaac gcctccggca tgtacagcat gcagattgag gccgggactt
     4561 acagcgtgac agccagccat cctgaccacc agtccgcgac tcaggctggt gtggtcgttg
     4621 tgggtggcca gaccatctat tgcaatttca tcctgcattt cgatcctttt ccctttgggt
     4681 tttatgacag ctttgaggac catgaggatt tcgcgctgga attcgcgccc tggacctgcg
     4741 tggatgttga tctctctgaa acttccggta tcagcggcgt caactggccc catgccggag
     4801 aaccccaggc tttcatcatt ttcaacccca cagccaccat ccctcccctt agcgggatcc
     4861 aggcctattc aggcgacaaa atggctgcca gtttccctgc cacccagtct cccagctcgg
     4921 attggctgat caccccagcc gtgaagggca tggatttctg ccagttccgg gcgcgctcac
     4981 tgaacgcgga caatggtttg gaacgtatca aagtgggggt atcaaccttt ggaaccgtcc
     5041 cccaaagctt cgttatcatc agcggagccc aagccctgga ggttccggcg tcctggaccc
     5101 agtacatttt cgatctgtcc ggtttcgagg acgagatcgt gtatgtgggg atccatcact
     5161 gcaccgacgg gggatcggct ttgctggtgg atagcttcaa cgccgaaaac gtgccggtgg
     5221 cggatgacag ccaaaccccg gccttgacgg ggattgaggg caatttcccc aaccccttca
     5281 acccggagac caatatccgc ttcagccttg cccggtcagg gccggtggaa cttggtgtct
     5341 tcgatttgag gggccgaaag cttaaaaccc tggtcaattc ttaccttccc tcgggacatc
     5401 acaccactgt ctggaacggg cttgatgatg ccggacggca ggtttccagc ggtgtttatc
     5461 tatgtaagct gcgtgccggt ggacagcgtt cctgccacaa gctggtattg atgaaataag
     5521 gctcatgttc aaattgagtg tgtgagttta ccctcagggt tcctttttgg ttgagtacct
     5581 acaccctctt gatttaatgg agagccataa ccagcttatc catagcaata tcctgccagc
     5641 caggtcctta gcgcccgagt ccatccctct cccatgcctc ccgtccagtc ctcgcatcaa
     5701 atgcgggcat cgtgcgggag gcggatcagg gccataggac agggccttaa cggccag
//
LOCUS       DAOOWR010000076         5756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14116_length_5756_cov_4.168918, whole genome shotgun sequence.
ACCESSION   DAOOWR010000076 DAOOWR010000000
VERSION     DAOOWR010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14116_length_5756_cov_4.168918"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLB85_02880"
     CDS             complement(<1..717)
                     /locus_tag="PLB85_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HOU72301.1"
                     /translation="MDRNLIEQVRHANDIVNVIQGYLPLKRAGANWRGICPFHQDTNP
                     SLNVNPARQIFKCFACGKAGNVFTFVQEYEKLTFFEAFKKLAARAGIAIPDSDRTKTV
                     STKRDQLLQIYTTARDFYCENLFKHGQKVLDYLQKRSFSPETAKSLELGYSLASEKSL
                     LNHLLKEGHSVSLLKESGLFGNYSGNLVDFFRGRLMFPIHSNIGEVIAFGGRVLEEGA
                     PGGKYVNTAGTELYTKGHELY"
     gene            complement(718..3066)
                     /locus_tag="PLB85_02885"
     CDS             complement(718..3066)
                     /locus_tag="PLB85_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOU72302.1"
                     /translation="MNAPELYADLEYARLAEQVAVRCHSQLGKDLSAGLAPLTDKKAI
                     EASLRLVGELQECLEHGLEKDFSDLADLGPLFREAQYSLFAYEEFHLVWLNARLASGI
                     AASASALETFPSLRRLWLKISALDWVCERFNRIFDPEGEVLDSASPELSRIRKRQASL
                     QRGIMKTMQGMLGDPRLEGYLQDKFITRRDDRYVLPVRESAAPHVPGIVQSQSGSGST
                     LFIEPQSVVPLNNELQLLRQEEKREIFRIFSEFTAQIKAQEKVLLANQEAMAALDFRY
                     ACARLCRSLKAEVPRILEQPVLKLSSARHPLLILRLGQPADVVPFDLSLGYESRIVLL
                     SGPNTGGKTVLMKAAGLLTLMALSGLPIPAGNDSEIGLFSQVFADIGDDQSIENALST
                     FSSHIQKIGRMLVQADSNSLVLIDEIGAATDPQQGSALAQAILERFTEIGCKAIVTTH
                     YTALKIFAETHPACLNASMQFDLNSLTPTFRFAPGIPGDSFAIEVASSLGLFPELIER
                     AKSLSGSQNLEFTELLKKMQAEKKALGRASYEYQLKTRNLEAKLNKLESREGKLEEEL
                     KQRKQKFIKELQSELISQQKLYQQEQEQLRKLDREERKALSERKLHEISGKLQDIRDE
                     LAAAGTKNQSLARDPKPGDRVWLAGFETEAILLEIRDSSANVDMNGISFRVPVDSLYE
                     VFACPETRETQPLATTHATPKAATELKLLGLTFDEARPLIDEFLDNATLAGLHSLRIV
                     HGKGTGALRGKVRDYLKRKKTVVSIDTPVPSEGGSGVTLVKI"
     gene            complement(3063..3608)
                     /locus_tag="PLB85_02890"
     CDS             complement(3063..3608)
                     /locus_tag="PLB85_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU72303.1"
                     /translation="MGIIDWLILIILALSAFQGLRKGLASTLVRVGAVIAVFMLIGQI
                     FPLVKNGLIENLRLGLVPATLLAALLIIVVVAVMVGIVNLIFKKVLKVTKLSGLNKFL
                     GLVFGFLNGLMVVIVLMVLLDYIPKLSTPLKDGSRHRVYVAVDTFKEDLFTVLKFEER
                     DRFQQIKAKLKKDNNQTQTGK"
     gene            complement(3703..3909)
                     /gene="rpsU"
                     /locus_tag="PLB85_02895"
     CDS             complement(3703..3909)
                     /gene="rpsU"
                     /locus_tag="PLB85_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU72304.1"
                     /translation="MPTVVSKENESFDYLLKRFKKKCEKAAILSEIKKRQAYEKPSVK
                     KKREENASKRKMLKIQRRMQRYMR"
     gene            4031..4165
                     /locus_tag="PLB85_02900"
     CDS             4031..4165
                     /locus_tag="PLB85_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72305.1"
                     /translation="MARHELSPGMHQVSFDGDGLPTGVYLYSRQCGAYRETKKLVLLK
                     "
     gene            4625..5107
                     /locus_tag="PLB85_02905"
     CDS             4625..5107
                     /locus_tag="PLB85_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOU72306.1"
                     /translation="MELRLEQLQFPTDTNIILGQSHFIKTVEDLYEALVNSVPGIKFG
                     LAFCEASGPRLVRKEGTDNELIECAVANMLRLGAGHSFLIVLRDTFPVNVLPAVIACR
                     EVVRIFCATANPVQVVLAHSEQGNGILGVIDGSSPLGIELDTDITHRKKFLRDIGYKR
                     "
     gene            5108..5659
                     /gene="thpR"
                     /locus_tag="PLB85_02910"
     CDS             5108..5659
                     /gene="thpR"
                     /locus_tag="PLB85_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU72307.1"
                     /translation="MLYRTFIALEVPDRPRACLAEKLRWLRGTPGVKWVREDNLHLTL
                     LFLGDVDSARIPDLARVMTEAAGRSAPFELALKGLELFPWKSPRLIWASLEALDDSLS
                     RWHKHLLSEIRTEGFKPDAKPLKLHITLGRIKSTLPPTLEREILQSEVEQSSFTYDRI
                     TLYRSVLKLEGPTYHSLEQCILR"
BASE COUNT         1242 a         1472 c         1787 g         1255 t
ORIGIN      
        1 gtaaagctcg tggcccttgg tgtaaagctc ggttccggcg gtgttaacgt attttccacc
       61 gggggcgcct tcttccaaaa cgcgtccgcc aaaggcgata acctcgccga tgttgctatg
      121 gataggaaac atcaggcgcc cgcggaagaa atccacgagg ttccctgagt agtttccgaa
      181 gagaccggat tcctttagca gcgagactga atggccttcc ttgaggaggt ggttgagcag
      241 agatttttca ctggcgaggg agtaaccgag ctcgagggat tttgcagttt cggggctgaa
      301 agagcgtttt tggaggtaat cgagcacctt ctggccgtgt ttgaagaggt tttcgcagta
      361 gaaatcccgg gcggtggtgt aaatctggag cagttggtcg cgcttggtgg agacagtttt
      421 ggtgcggtcg gaatccggaa tggctatccc ggcgcgggca gcaagttttt tgaaggcctc
      481 gaaaaaggtg agcttctcgt attcctgcac gaaggtgaag acgtttccgg ccttgccaca
      541 ggcgaagcac ttgaagatct gccgggcggg gttcacgttc agggaggggt tggtgtcctg
      601 gtgaaagggg cagataccgc gccagttggc gcccgcgcgc ttcaggggca gatagccctg
      661 gataacattc acgatatcat tggcgtggcg gacctgttcg atgaggtttc tatccatcta
      721 tattttcacc agggtgaccc cgctgccgcc ctcggagggg actggcgtgt cgatcgagac
      781 caccgttttt tttcttttca gatagtcgcg caccttgccc ctgagggcgc cggtgccttt
      841 gccgtggacg atccggaggc tatggagccc ggcgagggtg gcgttgtcca aaaactcgtc
      901 tatcaacggc cgggcctcgt caaaggtgag gccgaggagc ttgagctcgg tggcggcctt
      961 gggcgtggcg tgggttgtgg ccagaggctg ggtttcccgg gtctcggggc aggcgaacac
     1021 ttcgtaaagg ctgtccaccg gcacgcggaa gctgatgccg ttcatgtcca cgttggcgct
     1081 actgtcacgg atttccagga ggatggcttc ggtttcaaaa cctgccagcc agacccggtc
     1141 tccgggcttg ggatcgcggg ccagggattg gtttttggtc ccggccgcgg cgagttcatc
     1201 acggatgtcc tgcaatttac cggagatttc atgcaatttc ctttccgaaa gggccttgcg
     1261 ttcttcccga tcgagcttgc ggagctgttc ctgttcctgc tggtaaagct tttgctggga
     1321 gatgagctcg ctctggagct ctttgatgaa cttctgcttg cgctgtttca gctcttcctc
     1381 gagcttgccc tctcggcttt ccaatttgtt cagcttggcc tcgaggttgc gggttttgag
     1441 ctggtattcg taggaggcgc ggccgagggc ctttttttct gcctgcatct tcttcaggag
     1501 ttcggtgaat tcgaggttct ggctgccgct gaggctcttg gcacgttcga tgagctccgg
     1561 gaacaggccc aaggaggaag ccacctcgat ggcgaagctg tcgccgggaa tccccggggc
     1621 gaagcggaaa gtgggggtaa ggctgttcaa atcgaactgc atggaggcgt tgaggcaagc
     1681 gggatgggtt tcagcgaaaa tcttgagggc ggtgtagtgg gtggtgacga tggccttgca
     1741 gccgatttcc gtgaagcgct ccagaatggc ctgggcaagg gcggaaccct gctgggggtc
     1801 ggtggcggca ccgatctcat cgatgagcac caggctgttt gaatccgcct ggacaagcat
     1861 ccggccaatt ttctgaatgt gcgaactgaa ggttgagagg gcgttttcga tgctctggtc
     1921 atcaccgatg tcggcaaaaa cctggctgaa gaggccgatt tcgctgtcat tgccagcagg
     1981 gatgggcagg ccggaaaggg ccatcagggt cagcagtccg gcggctttca tcagcacggt
     2041 cttaccgccg gtattgggtc cgctgagcag tacgatcctg gattcatagc cgagggaaag
     2101 gtcaaagggc accacatcgg caggctggcc caggcgcagg atgagtaggg gatggcgggc
     2161 tgaggagagc ttcaggacgg gctgttccag gattcggggc acttcggctt tcagggagcg
     2221 gcagagccgg gcgcaggcat agcggaaatc cagggcggcc atcgcctcct gattggccag
     2281 caggaccttt tcctgggctt ttatctgggc tgtgaattcg ctgaaaatgc ggaagatttc
     2341 acgcttttct tcctgcctga gcaactggag ctcgttattg agcggcacca cggactgggg
     2401 ctcgatgaag agggtagagc cgcttccact ctggctttgc acgatgccgg gcacgtgggg
     2461 ggcagcgctt tcgcgcacgg ggaggacata gcggtcgtcg cgtcgggtta tgaatttgtc
     2521 ctggagatag ccttcgaggc gggggtcgcc cagcattccc tgcatggtct tcatgatccc
     2581 gcgctgaagc gaagcctggc gtttgcggat gcgcgaaagc tccggcgagg cggaatcaag
     2641 cacctcgccc tcgggatcga aaatgcggtt gaaacgttcg caaacccaat ccagggcgga
     2701 gattttcagc cagaggcggc gcaaggaggg aaaggtttcc agggcggagg cagaggcggc
     2761 tatcccactg gcaaggcggg cgttgagcca aacgagatga aattcctcgt aggcaaagag
     2821 ggaatattgg gcttcgcgga agagggggcc aaggtcggcc aagtcgctga aatccttttc
     2881 caggccgtgt tccaagcatt cctgaagctc gcccaccagg cgcaacgagg cctcgatggc
     2941 ctttttatcg gtcaggggcg cgagtccggc agagaggtcc tttcccaact ggctgtggca
     3001 gcggacggcc acctgttccg ccagccgggc gtattccagg tccgcgtaaa gctcgggggc
     3061 gttcattttc cggtctgggt ctggttgttg tcctttttga gcttcgcctt gatctgctga
     3121 aagcggtcgc gttcctcaaa tttgaggacg gtgaagaggt cttccttgaa ggtgtccacg
     3181 gcgacgtaaa cccgatggcg ggagccgtcc ttgagtgggg tggacagttt cgggatgtag
     3241 tccagcagca ccatcagcac gatcaccacc atcaggccgt tcaggaaacc gaagaccagg
     3301 cccaggaact tgttcaggcc ggatagcttc gtgaccttga ggactttctt gaagatgagg
     3361 ttcacgatgc ccaccatcac cgccaccacc acgatgatga gcagcgcggc aagcagggtg
     3421 gccggaacca ggcccagcct gaggttttct atcaggccgt ttttcaccag gggaaaaatc
     3481 tgtccgatga gcataaagac ggcaatgacg gcgccgacgc gcaccagcgt tgacgccaaa
     3541 cccttgcgaa gcccctgaaa ggccgacagg gcgagtatga tcaggatgag ccagtcgatg
     3601 atacccatga tttacctctt tttttatggg aataaaaaag caaacaggga cgaatccctg
     3661 ttgcaaacgg ttcattaggc aaaataacgg aaacgcccgc ggctacctca tgtagcgctg
     3721 cattctgcgc tggatcttga gcatcttgcg tttggaagcg ttttcttcgc gttttttctt
     3781 cacgctgggc ttctcgtagg cctgtctctt cttgatctca gaaaggattg cggctttctc
     3841 gcatttcttc ttgaaccttt tcaacaggta atcgaaagac tcattctctt tggaaactac
     3901 tgtcggcaaa caaatcacca cctttttttc taaattgaga aggcagaatt ttggaagggg
     3961 ggaattgtgt caagaggaaa atgaagggct ttcccgaaaa atcagcatct ggagtgacgg
     4021 ctgacaaagc gtggcccgcc atgaactgag ccccggcatg caccaggttt ccttcgacgg
     4081 ggacggactt cccacggggg tctatcttta cagccggcaa tgcggtgctt acagggaaac
     4141 aaagaaactg gtgctgctga aatagggtgg gtgtccacga attacacgaa ttgggaaagt
     4201 gattaagtga tggattgatg ggtgatagag ggaaaaggag aaagaaataa aaaagttggc
     4261 ctggcgtaca gcgaacgtag tgagcatgga cgacaggatg tattgaggta aatcattaat
     4321 tgctgacgtc gatgctgcgc tcgacgccag catatggcca ggcgatatct gctggccatt
     4381 aaggcccttt cccgtgttcc tgtttcgcct cccgcatgat gcccgcattc gatgcgggga
     4441 ttgtgcagga ggcgtaagag aaggaagggc acgagggttc agggcctgac cgacaggtca
     4501 tcacgggaaa aaagcagtaa gtaaccggtg tgacagacaa agagaaacaa aacgggaaat
     4561 ttagcttgac aaggctctgc acacggaata tcttgccgac atcagataaa aagaggtttg
     4621 cagcatggaa ctcaggcttg agcagcttca atttcccaca gacaccaaca tcattctggg
     4681 tcaaagccac ttcatcaaga ccgtggaaga cctctatgaa gctttggtta acagcgttcc
     4741 cggcatcaaa ttcggcctgg ccttctgcga ggcctcaggt ccccgcctgg tgcgcaaaga
     4801 gggcacggac aacgagttga tcgaatgcgc ggtggccaac atgctgcgcc tgggcgcggg
     4861 gcacagcttc ctgatcgtgc tgcgcgacac tttccccgtg aacgttttgc cggcagtgat
     4921 agcctgccgc gaggtggtgc ggattttctg cgccacggcc aatccggtgc aggtggtgct
     4981 ggctcacagc gaacagggaa acggcatttt gggcgtcatc gacggttcct cgcccttggg
     5041 aatagagctg gataccgata tcacccaccg caaaaaattc ctgcgggaca tcggctacaa
     5101 gcgctgaatg ctttaccgaa ccttcatcgc cctggaagtt ccagacaggc cgcgagcctg
     5161 ccttgccgaa aaactgcgct ggctgcgggg aacgcccggc gtgaagtggg tcagggaaga
     5221 caatctgcat ctgaccttgc ttttcctggg tgatgtggac agcgcccgaa tccccgatct
     5281 ggccagggta atgaccgaag cggcgggacg aagcgcgccc ttcgaactgg cattgaaagg
     5341 actggaactc ttcccctgga aatcgccgcg gctcatctgg gccagcctgg aggcgctgga
     5401 cgactccctt tcccgctggc acaagcatct gctatctgaa attcgcacag agggtttcaa
     5461 gcccgacgcc aagccgctga agctacatat cactctggga cggatcaaat ccaccctgcc
     5521 gcccacgctg gaacgcgaaa tattgcaaag cgaggtggaa cagagcagct tcacctatga
     5581 cagaatcacc ctctaccgca gcgtgctcaa gctcgagggg cccacctacc acagtttgga
     5641 acaatgtata ttaagataaa caaaaggata tgacaatgca ggacaaaaac aaagagacag
     5701 ccctcaagac cgcgatttcg cagcttgaga aaaaatacgg cgtgggcacg ctgatg
//
LOCUS       DAOOWR010000077         5740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14163_length_5740_cov_3.738083, whole genome shotgun sequence.
ACCESSION   DAOOWR010000077 DAOOWR010000000
VERSION     DAOOWR010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14163_length_5740_cov_3.738083"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /gene="purL"
                     /locus_tag="PLB85_02915"
     CDS             complement(<1..1519)
                     /gene="purL"
                     /locus_tag="PLB85_02915"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOU72308.1"
                     /translation="MEIKITKELIEQHGLSEEEYARIKEILGRTPTYVELGIFSVMWS
                     EHASYKNSIKLIKTLPRDGKYLLAKAGEENAGVVDIGDGLAISFKIESHNHPSALEPY
                     HGAATGVGGILRDIFTMGARPIAALNSLRFGDPRSPRTLHLIREVVRGIADYGNCFGV
                     PTVGGEVFFDEAYEENPLVNAMAVGLLRHEDLARSAASGIGNLVVYVGAKTGRDGIHG
                     ATFASVDLSEESAEMRTAVQVGDPFYEKLLLEATLEVIQAGLVVGIQDMGAAGLTCSS
                     SEMAGKGGVGIELDLEHVPIREKGITPYEIMLSESQERMLLIVEPANLQAVLEVFSKW
                     ELDASVVGRVTDDKLLRVRMNGETLAEIPAETLVLGGSAPVYEREQKAPKRPPDPDLG
                     SYPQPDDLGKVLLDLLAEPSIASKRAVFSQYDHMVQIGTKVEPGSDAAVIAIKGTQKG
                     IALATDCNARHCWLDPFEGAKGAVAEAARNVACSGGRPIAVTNCLNFGNPYNPEVY"
     gene            complement(1523..2608)
                     /locus_tag="PLB85_02920"
     CDS             complement(1523..2608)
                     /locus_tag="PLB85_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOU72309.1"
                     /translation="MVKFIRSRDLKDKTILHFTGGMYGIGCTVSSLEAIDRILNLKGR
                     TDLKGFIVLVPHLDWFAEEGVAIPDRILPLLEQYWPGNLTVVFKCDDERFKHLAVDGK
                     VAFRVPDDALLRLLIELLEEPIISTSINIASLPPENDFKRLTGMFENWFDIGIVPAPV
                     NVEYDAQPSTVVEYVTSRESKNQSGLDELRCLREGSIPFYGVRNSFLVPTILFVCTAN
                     ICRSPMAEKLFNHYIKEAGLNYIGDSCGLLPDGQPISAGSLQLLLEKGILEAQEHFSK
                     QFTPDMISGSRLVLTMEERQRDFLRGNEPNSVAKILTLNEYLGEPGDIADPFGSGIDT
                     YRKTFEIIDDRIKRLVSKLRTQANPLG"
     gene            complement(2659..3768)
                     /locus_tag="PLB85_02925"
     CDS             complement(2659..3768)
                     /locus_tag="PLB85_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU72310.1"
                     /translation="MKHRIIGIICLLLTVAVLCGDDLQDNQKKLDKVQKDLQAAQKKI
                     EQNERNKRKNENEISNSRRAKQKTDAEMRRSQEVAREKLQALNSVRTELRTVEEQIRD
                     LRTAQISQLDKMVRLSRRNKTLKMVHKDEHCLAILASKTRNKLITLGDAQSELSYEQS
                     QKGQEYSLANSDFQNKSSTSKNLAANVKKLEAQQSRLSREQKNLQNQIARLQQDAARL
                     EGLIASLASKPRSDDQEPASYKFSSRTIAWPLKGRIIRSYGQESRAYGTSVVSNGIDI
                     AAPEYTTVAAADAGVVVFSGSYGGQGKLLIIDHKNGFFTVYAYNNDLLVSTGTNVKKG
                     QAVAKSGMTGSASEPSLHFEVRKDGKAVNPLLYLE"
     gene            complement(4176..5138)
                     /gene="secF"
                     /locus_tag="PLB85_02930"
     CDS             complement(4176..5138)
                     /gene="secF"
                     /locus_tag="PLB85_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOU72311.1"
                     /translation="MRILQNVNFPFVQSRRITYVIAALALIASVVGIFVCGLNWSTDF
                     TGGVSAVINMKPKDPGVKPLNIDQLRDVIREGGFKESEIQFIGNPADATFQIKVEGSD
                     AEQIKEKILELVRDNLDDYVKGRNLQSDVLREVNTVGAKAGAEMRTNVITAVLIALLL
                     MIIYIWIRFEFTFGLMAIVALFFNVVLVVGVFAWTGKEITMQIIAALLTIVGYAINDT
                     IVVFDRIREDLRLYRKDPIESIFNRAINSTLSRTVITGGSTLLTAFALYFFGGAVIHD
                     FALAIILGIVIGTLSSIFIASNLVLDTIKSTRLEKKSLKHLSKK"
     gene            complement(5149..>5740)
                     /locus_tag="PLB85_02935"
     CDS             complement(5149..>5740)
                     /locus_tag="PLB85_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HOU72312.1"
                     /translation="SLAILLNNESLTAPIVLKNANQVSATLGTDSIKSGLIAGLVGLL
                     AVMIFMVFFYNLSGLVTNFILIFNVGFILAAMTIFGGTLTMPGIAGIILSIGMSVDAN
                     VLIFERIREEMDAGKSPRSAADSGFKRASVTIWDSNITTVIAAFVLYQFGTGPVRGFA
                     LTLTIGIVGSIFCALVFLRYLMDKLLLSGNRQTLSI"
BASE COUNT         1228 a         1522 c         1572 g         1418 t
ORIGIN      
        1 aatagacctc gggattgtag ggattgccaa aattgaggca gttggttacg gcgatcgggc
       61 ggccgccgct gcaggcaacg ttcctggcag cctctgccac ggcacccttg gctccttcaa
      121 agggatcaag ccagcagtgg cgggcgttgc agtctgttgc cagcgctata cctttctgcg
      181 tccctttgat ggcgatgacc gcagcgtcgc tgcctggctc caccttggtt cctatctgca
      241 ccatatggtc atattgggaa aacacggccc ttttggaggc gatggacggt tcagcgagca
      301 agtccagcaa aaccttgccg aggtcatccg gctggggata actgcccaaa tcaggatccg
      361 gaggcctttt cggggccttt tgctccctct cgtaaacggg ggcgctgcct cccaaaacca
      421 gggtttcggc ggggatttct gccagggttt caccattcat gcggacccgt aacagtttgt
      481 cgtccgtcac ccggccgacg acactcgcgt cgagttccca cttgctaaag acttccagca
      541 cagcctgtag gttggcaggc tcaacgatca gcaacatccg ttcctgtgat tccgagagca
      601 tgatctcata aggagtgatg cctttttcgc ggatgggaac gtgctcaagg tcaagctcga
      661 tgccaactcc gcccttgcct gccatttcgg aactggagca ggtaagcccg gcagcaccca
      721 tatcctggat tcccaccacc aggcctgcct ggatcacttc caaagtggct tccagcaaca
      781 gtttctcgta aaacgggtcg cccacctgga cagcggtgcg catctccgcg gattcttcac
      841 tgaggtccac agaggcgaaa gtggctccgt gaatgccgtc gcgccctgtt ttggcgccaa
      901 catacaccac caggttgccg atgccggaag cggcggaacg ggccaggtcc tcatgacgca
      961 gcaaacccac agccatagcg ttcaccagtg gattttcctc gtaagcttcg tcaaagaaca
     1021 cttccccgcc tacggtggga actccgaagc aatttccgta atcggcaatg ccgcgaacca
     1081 cttcacggat cagatggagc gttcggggag accggggatc accaaaacgc agtgaattca
     1141 gcgccgcgat gggccttgcc cccatggtga aaatgtcgcg caggatgccg ccaacgccgg
     1201 tagcagcgcc gtgatacggt tccaaagcgg agggatgatt gtggctttcg atcttgaaag
     1261 aaatggccag cccgtcaccg atatccacca ccccggcatt ttcctcccct gctttggcca
     1321 ggaggtattt cccatcccgg ggcagggttt ttatcagttt gatggagttt ttgtagctgg
     1381 cgtgttcgct ccacatgacg ctgaaaatgc ccaattccac ataagtgggg gtccggccca
     1441 ggatttcttt gatccgggcg tattcttctt ccgaaaggcc gtgttgttcg atcaattctt
     1501 tggttatttt gatttccata tatcatccca aggggttggc ttgagtgcgc agcttggaga
     1561 ccaggcgctt gatccggtcg tctatgatct cgaaggtttt acggtaagtg tctattccgg
     1621 aaccgaaagg atcggcaata tcgcccggtt cgcccagata ttcgttcagg gtgaggatct
     1681 tggcgacgct gttgggctcg tttccacgca ggaaatcgcg ctggcgttcc tccattgtga
     1741 gcaccagcct gctgccgctg atcatgtccg gcgtgaattg cttggaaaaa tgctcctgag
     1801 cttccaggat gcccttttcc aaaagcagtt gcagcgatcc cgccgaaatg ggttggccat
     1861 cgggaaggag gccgcaggaa tcgccgatgt aattcagtcc ggcttctttg atatagtggt
     1921 tgaaaagctt ttcggccatg gggctgcggc agatgttggc tgtgcagaca aagaggatgg
     1981 tcggcaccaa aaatgaattc ctgaccccgt aaaaaggtat ggagccttcg cgcaggcatc
     2041 tgagttcatc cagcccgctc tggttcttgc tttcgcggct ggtgacgtat tcgacgacag
     2101 tggagggctg ggcgtcgtat tcaacattca ccggggccgg aacgattccg atgtcaaacc
     2161 agttctcaaa catcccggtg aggcgcttga aatcgttctc cgggggcagg ctggcgatgt
     2221 tgatgctggt gctgatgatg ggctcttcca agagctcgat caacaaacgc agcaaggcgt
     2281 catccggcac ccggaaggcc acttttccgt ccaccgccag gtgcttgaac ctctcgtcat
     2341 cacatttgaa aaccacggtt aggttccccg gccaatactg ctccagcagg ggcaaaatcc
     2401 tatcgggaat tgccaccccc tcttccgcga accagtccag atgcggaacc agaacaataa
     2461 accccttcaa gtccgtgcgc cccttgaggt tcagtatcct gtcgatcgcc tccagcgacg
     2521 ataccgtgca gccaatgcca tacatccccc ccgtgaaatg gaggatggtt ttgtccttaa
     2581 ggtctctgct tcggataaac tttaccaatc tccgcacgga aggttgtttc aacaggacca
     2641 agtactgcct cctcttccct attccagata taggagcgga ttgaccgcct ttccgtcttt
     2701 gcgcacttca aagtgcagcg aaggctcgct ggccgagccg gtcatgccgg atttggcgac
     2761 ggcttggcct tttttgacgt tcgtgccggt ggacaccagc aaatcgttgt tgtaggcata
     2821 cacggtgaaa aagccgttct tgtggtcgat tatcaggagc ttgccctgtc cgccataaga
     2881 cccggaaaac acaaccactc cagcgtccgc tgccgcgacg gtggtgtatt caggggcggc
     2941 aatgtctatt ccgttgctca ccaccgaggt tccgtaagct ctggattcct ggccgtagct
     3001 acggatgatg cgccctttca gcggccaagc aatggttcgg gagctgaatt tgtaggaagc
     3061 gggttcctgg tcgtcggacc tgggtttgga agccaggctg gcgatgagcc cctcaagcct
     3121 ggcagcgtcc tgttgcaatc tggcgatttg gttttgcagg ttcttctgtt ccctgctcag
     3181 gcgcgattgc tgggcttcga gttttttaac gttggcagcc aggtttttgc tggtggatga
     3241 tttgttttgg aaatcgctgt tggccaggct gtattcctgt cccttttggc tttgctcata
     3301 ggacagttcg ctctgggcat ctcccagggt tatcagcttg ttccgggttt tggaggcgag
     3361 aatggccagg cagtgctcat ctttgtgcac cattttcaat gttttgttgc ggcgggagag
     3421 gcgcaccatc ttgtccagtt gggagatttg ggcagtccgg aggtcacgga tttgttcttc
     3481 gacagtgcgt aattctgttc tcactgagtt cagggcttgc agtttctcgc gggccacttc
     3541 ctgactgcgg cgcatttccg cgtcggtctt ttgtttggcc cggcggctgt tgcttatttc
     3601 gttttcattc ttccttttgt tgcgttcatt ctgctcgatc tttttctgag cggcctgcag
     3661 gtccttctga accttgtcca gctttttctg gttgtcttgc aggtcgtctc cgcaaagcac
     3721 ggcaaccgtc aacagcaggc aaatgatgcc aattatccta tgtttcatat tttatacctg
     3781 aattcgcttt aatgcccatg attaaaaccc atgccttgct gtcaacgcaa attttcttca
     3841 tacttgcagg ccattttcag gttcccctta aaatctgatg aattggctgg agatgcgaaa
     3901 tacaatggcg tgggtgcgac agacgtttag atgcgatgcc atctggccgt taaggccctt
     3961 tcccgtgtcc ctgaagcgcc tccctcatga tgcccgcatt cgatgcgggg atatggcggg
     4021 aggcacgcaa gggggataat catgagggct cagggcccga ctggaaggac attgttattg
     4081 ttaagcagtc aggctttctt tcattcagga agaaaatgaa gcctaaaaaa agccgcccca
     4141 caagaggcgg cttatcattg attctattgg atcgcttact ttttgctaag gtgcttaagc
     4201 gatttctttt ccaggcgggt tgatttaatg gtatccagaa ccaggttgct ggcaatgaag
     4261 atcgaagaaa gcgtgccgat cacgataccc agaattatcg ccaaagcgaa atcgtggata
     4321 acggctccgc cgaagaaata gagggcgaaa gccgtgagca gggtggagcc gccggtgatg
     4381 acggttctgc tcagagtgct gttaatggca cggttgaaga tggattcgat cgggtctttg
     4441 cggtataatc tcaggtcttc cctgatccgg tcgaaaacca cgatagtatc gttgatggca
     4501 taaccaacga tggtgagcaa ggcggcaatg atctgcatgg ttatctcttt tccggtccag
     4561 gcaaagacgc ccaccaccaa aaccacgttg aagaacaaag ccacgatcgc catgaggcca
     4621 aaagtgaatt caaagcgtat ccagatatag atgatcatca gcagcagggc gatcagcacc
     4681 gcggtaatca cgttggtgcg catttccgcg ccggctttgg cgcccactgt attaacttct
     4741 cgcaggacat cgctttgaag gttgcgaccc ttgacgtaat catccaggtt gtcgcgtacg
     4801 agttccagga ttttttcctt gatttgctca gcatcgctgc cttcgacctt gatctggaag
     4861 gttgcgtcag cgggatttcc tatgaactgg atttctgatt ccttgaatcc gccttcacgg
     4921 ataacgtcac ggagttggtc tatattcagg ggcttaactc cagggtcctt gggtttcatg
     4981 ttgatgacgg cgctcacgcc gccagtgaaa tccgtgctcc agttcagtcc gcagacaaag
     5041 atgcccacga cagaggcgat gagggctagg gctgcgatta cataggttat gcgtctggat
     5101 tggacgaagg ggaaattgac attctgtagt attctcatcg ttaactcctt agatactaag
     5161 cgtctgcctg ttgccgctga gcaacaactt gtccatcaga taacgcagga acaccaaggc
     5221 gcagaaaatt gaaccgacaa tacctatcgt aagcgtgagg gcaaatccac gcaccggacc
     5281 agtgccgaat tggtaaagca cgaaggccgc gatgacggtg gtgatgttgg agtcccaaat
     5341 cgtaaccgag gcacgcttga atccggagtc agcggcggag cggggtgatt ttcccgcatc
     5401 catttcttca cgaatccgtt caaagataag aacattggca tccaccgaca ttccgatcga
     5461 aagaatgata ccggcgatac caggcatggt gagggttcct ccaaagatcg tcatcgccgc
     5521 aaggatgaag cccacgttga agatgaggat gaagttggtg accaggcctg agaggttgta
     5581 gaagaaaacc atgaatatca taacagccag caagccaacc agccccgcga tgagtccgct
     5641 cttgatgctg tcggttccca gcgtggcaga gacctggttg gcatttttca gcactatcgg
     5701 cgcggtcagg ctttcgttgt tcaaaagaat ggccagggac
//
LOCUS       DAOOWR010000078         5720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14232_length_5720_cov_3.405472, whole genome shotgun sequence.
ACCESSION   DAOOWR010000078 DAOOWR010000000
VERSION     DAOOWR010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14232_length_5720_cov_3.405472"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..533
                     /locus_tag="PLB85_02940"
     CDS             288..533
                     /locus_tag="PLB85_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72313.1"
                     /translation="MPGYSNPEISKLGEEKRLTSKRSENNVTQIEYPGTQGLAMGKPE
                     RLTKSAFPGISRPDRKERNNTCLRLHKLGKLIIRKEK"
     gene            533..1345
                     /locus_tag="PLB85_02945"
     CDS             533..1345
                     /locus_tag="PLB85_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72314.1"
                     /translation="MKIRTALMTVALVAMLVLSACALKEKALELSNVPVMGNGADFVK
                     VVDGSYMLKPVDGGLEMTINVELLKQLEGIVPDSLTIDNIGNWTISFKNAAGEAVMGM
                     ESVPLKEEHKAEIVALMQGLISDKKDLTFMQMIEDKAEVKRILGEVNSIELNTEFVYP
                     EVAMMPPTEVVETPATPVKPGQKPPVVQPPTPPAPPAPTVSDAKIDDFGKAVDRYLAL
                     KAKNPTTPREMTRLTQLRTEAQNQLNALAAEFENMTGAQKTKFNNLKTKLGQ"
     gene            complement(1838..2308)
                     /gene="rpsG"
                     /locus_tag="PLB85_02950"
     CDS             complement(1838..2308)
                     /gene="rpsG"
                     /locus_tag="PLB85_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU72315.1"
                     /translation="MPRKKKAVVREVLPDPKYNDVIVSKFMNCLMKQGKKSIAEQIVY
                     GAFDIIAEKTKQPPLEVFKTALENVRPMVKVVSRRVGGSNYQIPLEVNEKNGRALAFR
                     WLISYSRARSEKTMIERLAAELIAAYKKEGASIKKREDTHKMAEANKAYAHLRF"
     gene            complement(2324..2704)
                     /gene="rpsL"
                     /locus_tag="PLB85_02955"
     CDS             complement(2324..2704)
                     /gene="rpsL"
                     /locus_tag="PLB85_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007070674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU72316.1"
                     /translation="MPTINQLIRKGRTEIESKKKNRALMGCPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLVNGFEVTAYIGGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DSAGVEKRVNSRSKYGTKRPKTKK"
     gene            complement(2737..5655)
                     /locus_tag="PLB85_02960"
     CDS             complement(2737..5655)
                     /locus_tag="PLB85_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72317.1"
                     /translation="MTTLRHFYSTDEVIAAYESEEQNYSVKGEVIGARNLDLHTWIRV
                     KVDGNFIVTTVGRVIFNQVIPREVGFQNITYDKGKLNDLAMLCFDAVGQWRTAQFLDQ
                     LKEIGFGYATRAGVTFSFSDIVVPKRKDEIIGESEEDVKKVFDLHQKGGITESERYGR
                     IVDLWKKTTVRVTDEMMEDLSKSRNGLNSIYMMFKSGARGSKDQIKQLGGMRGLMDKP
                     SKIGSTGGADVIETPIKSNFRDGLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLV
                     DVAQNSIITIEDCGTVRGVNMSVLKEGNEVVTTLSDRIQGRTAVDDVVDPVTGKILVH
                     SGQEISNKMARTIQNHGIISVNVRSVLTCEAERGVCAKCYGRNLATQKPATIGDPVGI
                     IAAQSIGEPGTQLTLRTFHIGGAASTATEMAEVVSNHDGIVKFDRMNTVTNIDNQLIS
                     VSYLGRIFVMDENDENKVLEEYKVEYAATVHVRDGQKIALNTKLLSWDQFNNPLISTA
                     KGVIHYENFIKDITFREEYNDITFSRDITIIESKDRKKQPQFRIVSEDGSAVQVPLPA
                     GLVVRVEDGSFVHTGDILGQTSRMTIKQRDITGGLPRVQDLFEARVPKDKAKISDIEG
                     IVTIGGLKKAGRDIFVTPVNGILAPVEGKILVQSDEYLNQVVILTDKTVYTEKDGKVT
                     IIEEDKKKLISIAKRNSKQPVVYEIPKSLQIIVNNDDNVKVGAPLCGKVMPIPPLQES
                     IVNDGDMVKKGQPLAGRKYSIPSGKRIIVHQGDHVESGDALSDGPFDPHDMLVKGIIE
                     AQQMILNEVQEIYRKQGVKIDDKHISIIIRQMFKKVRITDSGSTSFLEGDIVDKIKVE
                     KENREIIQFGKTPAQFEQLLLGITKTSLLTDSWLSAASFQETTKVLTKAAIEGRVDQL
                     EGLKESIILGHRIPVGTGAKIYNTQIEEAVNNGETVAQIIDRFAHSSQDEEADDFYNF
                     "
BASE COUNT         1273 a         1458 c         1529 g         1460 t
ORIGIN      
        1 tgaagctcca agagaagggc gtattcaaca acccgctccg tattcagcct tgtccagtac
       61 aaaaaagttg aaatgcccat caccgccagg ttttcgccac cctttaccac gataaagacc
      121 cccgccaagc tatcctcgag ctgatgaccc gcgaactgaa agccgaatga tacagctcat
      181 gttccaatat tggtatacag tcccgaacgc cctttccacc atcacggcac cgggaaaaag
      241 gcagcccccc tctaattgaa aatgagccaa gaagatatga aaaaccattg ccgggctact
      301 caaatccgga gatcagcaag ctcggtgaag aaaaaagatt gacaagcaaa cgcagtgaga
      361 acaatgtgac tcaaattgaa tatccgggaa cacaaggatt ggcaatgggc aagcctgaaa
      421 ggctcaccaa atcagccttt cccggcatta gccgcccgga ccgcaaagag agaaataaca
      481 cttgtctccg gcttcataag ctgggcaagc taattattag aaaggagaaa taatgaagat
      541 cagaaccgca ttgatgacag tcgccctggt ggcgatgctc gtactcagcg cctgcgcgct
      601 gaaagagaaa gccttggaat tgagtaacgt tcccgtgatg ggcaacggag ccgattttgt
      661 gaaagtggtt gatggcagct atatgctcaa accagtggat ggcggcctgg aaatgaccat
      721 caacgttgaa ctgctgaagc agctggaagg aatcgttccg gacagcctta cgatcgacaa
      781 catcggaaac tggaccatct cattcaagaa cgccgcaggc gaagctgtga tgggaatgga
      841 atccgtcccc ctcaaggaag agcacaaggc tgagatcgtt gccctgatgc aaggcttgat
      901 cagcgacaag aaggatctca ccttcatgca gatgatcgaa gacaaagccg aagtgaaacg
      961 catcctcggc gaagtgaact ccatcgagct caacaccgag tttgtctatc ctgaagttgc
     1021 catgatgcct ccgactgaag ttgtcgaaac ccctgccacc ccggtgaaac ccggccagaa
     1081 accgcccgtc gtgcagcctc cgactccgcc cgcaccccct gccccgaccg tttctgatgc
     1141 caagatcgat gacttcggca aagccgttga tcgttacctg gccctgaaag ccaagaatcc
     1201 caccaccccc agggaaatga ccaggctcac acagctcaga accgaagctc agaatcaatt
     1261 gaacgctctc gccgccgaat ttgaaaacat gaccggcgcg cagaaaacca agttcaacaa
     1321 cctcaaaacg aaattaggcc aataactgat atcagactat caagacgaga cccggctgat
     1381 ttggccgggt ctttttttga ctttgttgca acctgatcag gcaattcgct gggttccaat
     1441 ttcgagatca ttgactgctg taaagccttg tttattatgt ttcatcttca aatatggtgg
     1501 tgagtcagat ggggacggtt ttctatagca tggagtgaag tggcttgaga ctccgcaaag
     1561 aaatgaagtg acgctggtct ggccgttaag gccctgtcat gcgtccctga tgcgcctccc
     1621 gtatgatacc cgcatttgat gcgagtatca tacgggaggc gtgggagggg gatggtcgcg
     1681 ggcgctaagg gcgtgactga caggatattg aggtgggtaa atacttatgg ctctatttca
     1741 aatcaattgg atgagagcgc ttaaacagaa agcagcccgg gaaacaagag gcggaaccga
     1801 aattccgcct cttgatatca gtgataaatt gcctggatta gaacctcaga tgggcataag
     1861 ccttgttggc ttcggccatc ttgtgggtgt cctcacgttt cttgatggac gcgccttctt
     1921 tcttgtaggc ggcgatgagc tcggcggcca gtcgctcgat catggtcttt tcgctgcggg
     1981 cgcgggaata gctgatcagc cagcggaaag ccagggctcg gccgttcttt tcgttcactt
     2041 ccagagggat ctggtagttc gatccaccca cgcggcggga aacgactttg accatcgggc
     2101 gaacgttttc gagggctgtt ttgaagactt ccagcggggg ctgcttggtc ttttcagcga
     2161 tgatgtcgaa ggcaccatag acgatttgct cggcgatgct ctttttgcct tgcttcatca
     2221 ggcagttcat gaatttggaa acgatcacgt cgttatattt gggatccggc agaacttcac
     2281 ggacgacggc ttttttcttt cttggcatgg ctcattcctc cgtttacttc ttagtcttgg
     2341 gacgtttggt cccatatttg gaacgtgaat tgacgcgctt ctcaactccg gcggaatcaa
     2401 gagctccgcg cacaatatga tagcgaacgc cggggaggtc tttcacacgg cccccgcgaa
     2461 ccagcacaat gctgtgttcc tgcaggttgt ggccttctcc gccgatataa gctgtaactt
     2521 caaatccgtt aaccagacgg acacgggcga ctttgcgcaa tgccgagttc ggttttttgg
     2581 gcgtcgtcgt gtaaacacga gtgcaaactc cgcgcctttg cggacatccc ataagcgccc
     2641 tgtttttctt tttactctcg atctcggttc tgccttttcg aatcagttga ttgatcgttg
     2701 gcactgattt acctccatgg ttttgtatgt ttggttttag aaattgtaga aatcatccgc
     2761 ttcctcatcc tggctggaat gggcaaagcg atcaatgatc tgggcgacag tttcaccgtt
     2821 gttgacggct tcctcgatct gggtgttgta gattttggct cccgtcccca cagggatacg
     2881 atggcccaga atgatgcttt ccttgaggcc ttcgagttgg tcgaccctgc cttcgatggc
     2941 ggctttggtg aggaccttgg tggtttcctg gaaggatgct gctgagagcc agctgtcggt
     3001 gagaagagag gttttggtga ttccgagcag caattgctca aattgagcag gggtcttgcc
     3061 gaactggatg atctcgcgat tctctttctc aacctttatt ttgtccacta tgtcgccttc
     3121 aaggaatgag gtgctgccgc tgtctgtgat gcgcactttt ttgaacatct ggcggatgat
     3181 gatgctgata tgcttgtcat cgatcttaac gccctgttta cgatagattt cctgcacttc
     3241 gttaaggatc atctgttggg cttcgatgat gcctttcacc agcatgtcgt gagggtcgaa
     3301 aggaccatcg gagagggcgt cgccgctttc aacatggtcg ccttggtgaa cgatgatgcg
     3361 tttcccagag gggatggagt atttgcggcc ggccagaggc tgaccttttt taaccatgtc
     3421 accgtcattc acaatgcttt cctgcagcgg cgggataggc attactttgc cgcatagcgg
     3481 agctcccacc ttcacgttgt catcgttgtt cacaatgatc tgcaggcttt tgggaatctc
     3541 atacaccacg ggttgcttgg aattgcgctt ggcgatggag atcagcttct tcttgtcttc
     3601 ctcgatgatg gttaccttgc cgtccttttc cgtgtaaact gtcttgtcgg taaggatgac
     3661 gacttggttc aaatactcgt cggactggac aagaatcttg ccttccaccg gcgctaagat
     3721 gccgttgaca ggagtcacga atatgtcgcg tcccgccttt tttagaccgc cgatggtgac
     3781 gattccctcg atatcggaga ttttggcttt gtccttgggc acgcgggctt caaagaggtc
     3841 ctgaacccgg ggcaggccac cggtgatgtc acgctgtttg atggtcatac gggatgtttg
     3901 gcccaggatg tccccggtgt gcacaaagct gccgtcctcc actctgacca ccaagcctgc
     3961 gggcagggga acctgcaccg cgctgccatc ttcgctcacg atgcggaact gcggttgttt
     4021 cttgcggtcc ttggattcga tgatggtgat gtcgcgggag aaggtgatat cgttgtattc
     4081 ttcacggaag gtgatgtcct tgatgaagtt ttcatagtgg ataacacctt tggccgtgga
     4141 aatgaggggg ttgttgaatt ggtcccagct gagcagcttg gtgttgaggg cgatcttttg
     4201 accgtcgcgc acgtgcaccg tggccgcgta ttcaaccttg tattcttcga ggaccttgtt
     4261 ttcgtcattc tcatccatca cgaagatacg gcccaaatag cttaccgaaa tcagctggtt
     4321 gtcgatgttg gtgacagtgt tcatcctgtc gaatttcacg attccgtcat ggttggagac
     4381 cacttccgcc atttctgtgg cagtggaggc cgccccaccg atatggaagg tacgcaaagt
     4441 cagctgtgtt ccgggttcgc cgatgctctg ggcagcgata atcccgacag gatcgcctat
     4501 ggtcgcgggt ttctgggtgg ccaggttgcg gccgtagcat ttggcgcaga cgccacgctc
     4561 ggcttcgcaa gtgagaacgg aacgcacgtt tacggatatg ataccgtggt tctgaatggt
     4621 gcgggccatc ttgttgctga tttcctggcc gctatggacc agaatcttgc cagtaacggg
     4681 atcgactacg tcgtcaaccg ctgtgcggcc ttggatccgg tcagacagag tggtaaccac
     4741 ttcgttacct tctttgagca cgctcatgtt cacaccgcga acagttccgc aatcttcaat
     4801 ggtgataatg gagttttggg ccacatccac cagacggcgg gtaagataac cggcgtcggc
     4861 tgttttcagg gctgtatcag ccagtccttt gcgggctccg tgagttgaaa cgaagtattc
     4921 cagcacggtc agaccatccc ggaagtttga tttgatcggg gtttcgataa cgtcagcacc
     4981 gccggttgag ccgatcttgg aaggcttgtc catcagtccg cgcataccgc caagctgttt
     5041 gatctggtct ttgctgccac gggcaccgga cttgaacatc atgtaaatgg agttgagtcc
     5101 gttcctgctt ttggaaaggt cttccatcat ttcatcagta acccggacgg tggtcttctt
     5161 ccacaggtcc acgatacggc catagcgttc gctttctgtg ataccgccct tttggtggag
     5221 gtcgaagacc tttttcacgt cttcctcaga ttccccgatg atctcatctt tgcgtttggg
     5281 aaccacaatg tcgctgaagc tgaaggtaac gccagcccgg gtggcatagc caaaaccgat
     5341 ctctttgagt tgatccagga actgggctgt gcgccactgt cccacagcgt caaaacaaag
     5401 catggccagg tcgtttagtt tgcctttatc gtaagtgata ttctggaaac caacctcgcg
     5461 cgggatcacc tggttgaata tcacgcgacc cacggttgtg acgatgaaat tcccatccac
     5521 tttcacgcgg atccaagtat gcaggtccaa attgcgggcg ccgatcactt ctcctttgac
     5581 ggagtagttt tgttcctcgg attcgtaagc ggcgatcact tcatccgtgc tgtagaaatg
     5641 acgcagggtg gtgatatcct tgggctgggg aaattcctca acggtaaggt agtagcagcc
     5701 taaaactata tcctggttgg
//
LOCUS       DAOOWR010000079         5715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14256_length_5715_cov_3.430035, whole genome shotgun sequence.
ACCESSION   DAOOWR010000079 DAOOWR010000000
VERSION     DAOOWR010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14256_length_5715_cov_3.430035"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="meaB"
                     /locus_tag="PLB85_02965"
     CDS             <1..942
                     /gene="meaB"
                     /locus_tag="PLB85_02965"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU72318.1"
                     /translation="AGVLSGDRTLLAKAITLIESNSPRHFEAGQELVRRLLPASGNSI
                     RVGVTGVPGAGKSTFIESFGLWLIERGHKVAVLAVDPSSSLSRGSILGDKTRMEELAR
                     HPSSFIRPSPSGGALGGVTRKTRETIIACEAAGYDIILIETVGVGQSEVTVRSMVDFF
                     LLMQIAGAGDELQGIKKGIMELADLVVVNKADGNNIQAAELAGRELNNALHYLRQATP
                     GWTTKALTCSAVQKTGLPEIWHEIERFVDQGKTSGVFEERRQSQVLQWFESLLTEAVL
                     NRFYSDPKITKQLPRLRAAVQDGSLPVLLAVQELLAD"
     gene            complement(1094..3256)
                     /locus_tag="PLB85_02970"
     CDS             complement(1094..3256)
                     /locus_tag="PLB85_02970"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72319.1"
                     /translation="MKTILLVMAATLLAAFLTAMASDPDAFVTALEVNSRMGITVFPA
                     TWDFENPTFPPQGFTCVDVDGAGTIWEVDTNHNTTPDGSKSALHYASDAGNQDGWLIL
                     PAVLMPEDSFMTLSFQHRSDWSENYQYCSLLANSSSDPQDPGWTEIWAANSVNTDWNR
                     VFVDVTDYIGEDTYFAFRYQGNDANYWYLDDITIYEAEPVTSLPVYWDFEGTEFPPAG
                     WSIQDNDGQPWYWLLDTEINHTPGGSNCAAHWYSSPWEGGYGQDGWLITPPILLPQLR
                     DQLVLDFWHNNFYMDCYAYSGVMIRPVNLAQLGWHELWNPDSVTEEWQNVRLNISPFA
                     GMMVHFAFVYRGYDAHSWFVDDITIRELTNDDQPPIITFLPPLSTPSPDVSFTLTAEV
                     VDDPIWQSGIDEVTLHYQATGTQIDLPMENISGNTWSATIPPQDTGTRVEYFITATDG
                     NLNTASTGDWDNFHWFVVDNYFWLFYNWNNSVSLGRQETFGAANRFANPFYELGSGML
                     LSEVAVDTPQPVQADLHIYSDDGFTLTDLTGPITYNFNGLNYYDLPDNLVINTPYFYV
                     SVENVPGGNGVWFTNDHDYGMSYWREAGYFSQVEEQGSWIIQAHVAAGLAAPEISFSL
                     WEGVPKLSWDPVPFANDYLIYASSDPYANDGYWTLLNTTTDTYYCPDYVEAQKFFRIK
                     ANNTPFEKAAWYPEHIPHAKKAIKPKRERADKASPAID"
     gene            complement(3774..4433)
                     /locus_tag="PLB85_02975"
     CDS             complement(3774..4433)
                     /locus_tag="PLB85_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72320.1"
                     /translation="MPAKIKYLVTLLLLLAFGTTLMAESTGLQLVQSLVLPGLSQIRH
                     GRNYGYGMLTAEAALISSMLYLNQEAQLKTREYYEYALKYAHIEPGDHPESYLRDISK
                     YNSSGYEAGGYNAWVYQEALRLFPHDPDQRQIYIDENIYPDNLAWNWDSVENRGQYVQ
                     IRTEAQNMRDYRMMAVGVMIVNHLISGIDVLRYNAKNKQAHVWLDIKDRSPVLMLGLD
                     F"
     gene            complement(4433..>4680)
                     /locus_tag="PLB85_02980"
     CDS             complement(4433..>4680)
                     /locus_tag="PLB85_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72321.1"
                     /translation="YPEEEAWSWQSSYHQDQYRSLRFRTQTAKMYRSLSLGVMLLNRV
                     ISLIDVALISRKPGQPTALYFTPLESGGLMLNYRMEF"
     assembly_gap    4681..4690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4691..5077)
                     /locus_tag="PLB85_02985"
     CDS             complement(<4691..5077)
                     /locus_tag="PLB85_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72322.1"
                     /translation="MRTSSKLLFLTLALLLALPLAALPKVAYLGMSAALPGSGEIALG
                     KSTRGGIMLTADLVALTAWLATGREIENLTSSYKSYADVYAGIPEGMPDSYYQDIQQY
                     ISSDAFNQVRILEARNFYLIYNYDPAG"
     gene            complement(5079..>5715)
                     /locus_tag="PLB85_02990"
     CDS             complement(5079..>5715)
                     /locus_tag="PLB85_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72323.1"
                     /translation="GTRYRRDYQKNVQTVMMNINPGALDIYDGTFFRLDDTDTQHFYE
                     DIGKYDKYVFGWADWYHNFATDPTSGNGSFYLSQVPNNATWVFNPNNTDPQLIINNRW
                     IKNYSIEYYMNNPGNLDPANAVAPGTNAASPWRQEYLDMRRSANQQFYYANYCTIGLA
                     LNHIAAAIDAFALTNRVNRTAITQKPFEFQYYTSLSSNNNITPSLGFTYRF"
BASE COUNT         1220 a         1459 c         1698 g         1328 t
ORIGIN      
        1 gccggggttc tcagtggcga ccgcaccctg ctggccaagg cgatcaccct catcgaaagc
       61 aattctcccc gtcatttcga ggcgggacag gaactggtcc gccggcttct gcccgcctcg
      121 ggaaactcca tccgcgtggg cgttacaggt gttcccggag cggggaagag caccttcatc
      181 gaaagcttcg gcctctggct aatagagcgg ggccacaaag tcgccgtgct ggccgttgac
      241 cccagcagtt cgctttcccg gggcagcatt ctgggcgaca agacccgcat ggaggagctg
      301 gcacggcacc caagcagctt catccgtccc tcacccagcg gcggagcctt ggggggcgtg
      361 acccgcaaaa cccgcgaaac catcatcgcc tgcgaagctg ccggctatga catcatcctc
      421 atcgaaaccg tgggagttgg acagtctgag gtaaccgtgc gctccatggt cgatttcttt
      481 ctgctgatgc agatcgccgg agccggagac gaactccagg gcatcaaaaa gggcatcatg
      541 gaactggccg acctcgttgt ggtgaacaaa gctgacggaa acaatatcca ggcagccgaa
      601 ctggctggac gtgaactaaa caacgccctc cattacctgc ggcaggcgac cccaggctgg
      661 accaccaagg ccctcacctg ctccgctgtc cagaaaaccg gcctgcctga aatctggcac
      721 gaaatcgaac gttttgtgga ccagggcaaa accagtggcg tgtttgagga acgacggcag
      781 agccaggtgc tccagtggtt cgaatccttg cttaccgagg cagttctaaa ccgcttctac
      841 tccgatccca agataacaaa gcaactgccc cgcctccgcg cggctgtgca ggacggttca
      901 ctgcctgttc tgctggccgt tcaggaactg ctggccgatt aacggaaacc tggaacaaat
      961 ctgcttatat ggccttattg acttcaaggg caggttgaat actgtcattg cacatgaagt
     1021 ccaagccgtc cataatctgt tcaattagtg atggttagct ttgacggctt gaactccatg
     1081 tcctcaattt ggtttaatct atcgccggtg atgctttatc tgctctttcc cttttgggtt
     1141 tgatggcctt cttcgcgtgc ggaatgtgtt ccggatacca ggcggctttc tcgaatgggg
     1201 tgttattggc ctttatccgg aagaactttt gggcttcaac ataatctggg caatagtagg
     1261 tgtccgtggt agtgttgagc aaagtccaat acccatcatt cgcgtaagga tccgaactgg
     1321 cgtagattag atagtcgtta gcaaacggca cgggatccca gcttagtttg ggaacccctt
     1381 cccacaggct gaagctgatt tccggagcgg cgaggccggc ggcgacatgg gcctggataa
     1441 tccaggagcc ttgttcctcc acttgggaga agtatccggc ttccctccag tatgacattc
     1501 cgtaatcatg atcgttagtg aaccaaacgc cgttcccgcc ggggacgttt tcgactgaaa
     1561 cgtagaaata gggagtgttg atcaccaggt tgtccggcag gtcgtagtag tttaagccat
     1621 tgaagttata ggtgattggc ccggtgaggt cggtgagggt gaacccgtca tcagagtaga
     1681 tgtgcagatc cgcctgcact ggctgggggg tgtcgacagc cacttctgaa agaagcatac
     1741 ccgaacccag ctcgtaaaag ggattggcga agcggttggc ggctccgaat gtttcctggc
     1801 gccccaggct taccgaattg ttccagttgt agaagagcca gaagtagttg tccaccacaa
     1861 accagtggaa gttgtcccaa tctccggtgc tggcggtgtt caagttcccg tctgtggcag
     1921 tgatgaaata ttccaccctg gtgcccgtgt cttggggcgg tatggttgca gaccaggtgt
     1981 ttccgctgat gttttccatg ggtagatcta tttgcgtgcc cgtcgcctgg tagtgcagag
     2041 tcacttcgtc tattccgctc tgccagatgg ggtcatcaac cacttccgcc gtgagggtga
     2101 aactgacatc aggactgggg gtgctgagcg gggggaggaa ggtgatgatg ggaggctgat
     2161 cgtcgttggt cagttcacgg atggtgatgt cgtccacaaa ccaactgtgg gcgtcataac
     2221 cccggtaaac aaaggcgaaa tgcaccatca tgcccgcgaa ggggctgata ttgagcctca
     2281 cattctgcca ttcctcggtt acgctgtcag ggttccaaag ctcatgccag ccaagctggg
     2341 cgaggtttac ggggcggatc atcaccccgc tgtaggcgta gcagtccata tagaaattgt
     2401 tgtgccagaa atcgagcacc agttgatcgc ggagctgcgg aagcaggatg ggcggggtga
     2461 tcagccagcc gtcctggccg tatccacctt cccaggggga gctgtaccaa tgggcggcgc
     2521 aattcgagcc gccaggagtg tggttgattt cagtatccag cagccagtac cagggctgac
     2581 cgtcattatc ctggatagac cagccggcgg ggggaaactc agtcccttca aaatcccagt
     2641 aaacgggcag ggaggtgaca ggctccgcct cgtagatggt gatgtcgtcc agataccagt
     2701 agttggcgtc attgccctga tagcggaagg caaaataggt gtcctcgccg atatagtcgg
     2761 tgacatccac aaagacgcgg ttccagtcgg tgtttacgct gttggcagcc cagatttccg
     2821 tccagccggg atcctgggga tcagaggagg agtttgccag caggctgcaa tattggtagt
     2881 tttccgacca atcgctacga tgctggaagc tgagggtcat gaagctgtct tcaggcatca
     2941 gcaccgccgg aaggatcagc cagccgtcct ggtttccggc atcgctggcg tagtggaggg
     3001 cggatttgct tccatccggg gtggtgttgt ggtttgtatc gacctcccag atggttccgg
     3061 ctccgtcaac atcaacgcag gtgaatcctt gcggcgggaa ggtgggattc tcaaaatccc
     3121 aggtggcggg aaaaacggtg atccccattc tactgttcac ctcaagggcg gtcacaaagg
     3181 catcaggatc actggccatg gcggtgagga acgcggcgag cagcgtcgct gccattacta
     3241 gcaagatcgt tttcatacat gaactccttt gtttaaggtt tgaacaggca ttccgggccg
     3301 tgcgggcctt ccggggagtg cggacggggc gcggagagga gaccggtcct caaggttccg
     3361 aagccgttct gcggagtttg tatgctagta atcaagcagg cccgcggcct gatatgctac
     3421 atcgtacgct tgtgaagtgt tgtaattatt gtgcagcata tttaatcgtc aaggataaag
     3481 tggaccaaca tcagactttt ttgtggattt tttgcgtcag gacccgctga actcaatccg
     3541 gatggcgggg ataggggcaa accggatgga caaactcaaa cgggctggaa caaccggatt
     3601 acccatttga tatgaaagag cgccacaaac tgtttaccca ccacgatgcc ctgccagtca
     3661 cacccttagc gctcacgttt atccatctcc cacgccccac gcaaaatact cgcatccaat
     3721 gcgggcatca ggcgggaggc gcttcaggga ccaggtatag ggccttaacg gctttagaag
     3781 tccaagccta acatcaggac gggactgcgg tccttgatgt ccagccagac atgggcttgt
     3841 ttgtttttgg cgttgtagcg caacacgtct atgccgctga tgaggtggtt cacaatcatc
     3901 acgcccaccg ccatcatcct gtagtcgcgc atgttttggg cttcggtgcg aatctgcaca
     3961 tactggcccc ggttttcgac gctgtcccag ttccaggcaa ggttgtccgg gtagatgttc
     4021 tcgtcgatgt agatctgtcg ctggtccggg tcgtggggaa acagccgcag cgcttcctga
     4081 tacacccagg cgttgtatcc gccagcctcg tagccgctgc tgttgtattt gctgatgtcg
     4141 cggagatagc tctcagggtg gtctccaggc tcgatgtggg catactttag cgcgtattcg
     4201 taatactcgc gggttttcag ctgcgcctcc tgattcagat agagcatgct ggatatgaga
     4261 gcggcttcag cagttagcat gccgtagccg taattccggc cgtggcgaat ctggctgagg
     4321 ccgggcaaaa cgagtgactg cacgagctgc aatcccgtgc tttcagccat cagagttgtc
     4381 ccgaaggcta aaagcagcag gagggttacc agatatttga tcttggcagg catcaaaact
     4441 ccatacggta attcaacatc aggccgccag actcaagcgg cgtaaaatag agggctgtgg
     4501 gttggcccgg ttttctggaa atcagggcaa cgtcgatgag gctgataacg cggttcagca
     4561 gcatcacgcc caggcttagg ctccggtaca ttttggcggt ttgggtcctg aaccgcaggg
     4621 aacggtattg gtcctgatga taggaacttt gccaggacca ggcttcctct tcgggatagg
     4681 nnnnnnnnnn gccggcagga tcgtaattgt atatcaaata aaagtttcgt gcttcgagta
     4741 tcctgacctg gttgaaggcg tcgctgctaa tatactgctg gatatcctga tagtagctgt
     4801 ccggcatgcc ttcgggaatg ccggcataga catcagcata gcttttgtat gaacttgtga
     4861 ggttttctat ttcacgtccg gtggcaagcc aggctgtgag ggccacgagg tcagctgtca
     4921 gcatgatccc tccgcgggtg cttttgccca gggcgatctc accgcttccg ggcagcgccg
     4981 cggacattcc cagatacgcc actttgggca acgcggccag cggcaaagcc agcaacaaag
     5041 ccagggtcag gaacagcagt tttgaggatg tgcgcatctc agaacctgta ggtaaagccc
     5101 aaagagggag tgatgttgtt gttgctgcta aggctggtgt aatattggaa ctcaaagggt
     5161 ttctgggtga tggcagttct gttgacgcgg ttggtaaggg cgaaggcgtc gatggctgcg
     5221 gcgatgtggt ttagggcaag gccgatggtg caataattgg catagtagaa ctgctggttg
     5281 gcggagcggc gcatatccag atattcctga cgccagggtg aggcggcgtt ggttccgggc
     5341 gcaaccgcgt tcgcgggatc gaggttgccg ggattgttca tgtaatattc aatactgtag
     5401 ttctttatcc agcggttgtt gattatcagt tggggatcgg tgttgttcgg gttgaagacc
     5461 caggttgcgt tgttgggcac ttggctcaga tagaaagaac cgtttccgga agtgggatcg
     5521 gtggcgaaat tgtgatacca gtcagcccag ccgaacacgt atttgtcgta tttaccgata
     5581 tcttcataga aatgctgggt gtcggtgtcg tccaggcgga aaaaagtgcc gtcgtagata
     5641 tccagcgcgc ccggattgat gttcatcatc accgtttgca cattcttctg gtaatccctg
     5701 cggtagcggg tgccg
//
LOCUS       DAOOWR010000080         5707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14285_length_5707_cov_3.297948, whole genome shotgun sequence.
ACCESSION   DAOOWR010000080 DAOOWR010000000
VERSION     DAOOWR010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14285_length_5707_cov_3.297948"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1383)
                     /locus_tag="PLB85_02995"
     CDS             complement(244..1383)
                     /locus_tag="PLB85_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU72324.1"
                     /translation="MKKNRNTSYIVSIDKTILGCYLALLVIGLLVMLDISSVQSSMVN
                     YYRHAIFVLISIFATIITLYFVKVDKLRPLNRWLVYLIIGLLVLVIFTGTKTKGAERW
                     LMLGPVGIQPSTLARLVLILYFAGYLDTKQDKIYLANVKELVIEFIPLILYTTVIFIL
                     IFLGKHLSTLIICGATLLGMLVYAGLRKRFVLGMLMLGMIAGTVVITKGDSFRFDRIN
                     TYKVYSLLIPNRPEPSNSPEEYQVRESLTALTSGGFLGTGMAHGRAKHYFLPEARTDY
                     IYTIIGEEFGFLGAALVLLLHALLFFRIIKLAETQENRYHKYLMAGLGMNIFLNALIN
                     TGVSMSIIPATGNTLPFVSYGGTALLVDSISIGLVLNLTAKRRQM"
     gene            complement(1370..2725)
                     /gene="murD"
                     /locus_tag="PLB85_03000"
     CDS             complement(1370..2725)
                     /gene="murD"
                     /locus_tag="PLB85_03000"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU72325.1"
                     /translation="MFDASRKYAILGLARSGIAAAYKIRELGGTAFLSDAQPIEKISG
                     ADTIASDFDCEFGGHSDRLFQCDTWIVSPGIPLNLPIIAKARAKGIELISEIELGFRV
                     KSSDSKVVAVSGSNGKSTTVSLIHHILKNMGYNSILAGNIGSAFCCWPIEKPGIDFIV
                     LEVSSFQLDLIDSFRPDVAVLLNITPDHLDRYASFADYAASKFRLFTNQTQTDTAVTC
                     LDSEPVVERQHLIKSRLLRYSLTKSLPDCEAWLNRDAIQIGLRHKLPISELKIRGPHN
                     HANAMAALLTVDALTHDISLAIEAAKSFEPLSHRLEFVAIINGIFFYNDSKATNTDSV
                     RSALTSFDQPIRVIMGGSDKGENFSVLTELLKQRARKVYLTGGTMEKMRAAWEGKLEL
                     DCVDDFETCIRAAFADSVPGDIVVLSPACASFDHFRNYEHRGEVFKEIVHIIRSEYEK
                     E"
     gene            complement(2739..3839)
                     /gene="mraY"
                     /locus_tag="PLB85_03005"
     CDS             complement(2739..3839)
                     /gene="mraY"
                     /locus_tag="PLB85_03005"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU72326.1"
                     /translation="MLYHLLYPLSRYSIVFNVMRYITFRSLGAFVTALLFTLMAGPSF
                     IRLLKRKAAVETIDADVPEKHRAKAGTPTMGGLLIIFGLLIASLLWNDLTNRSIIMMF
                     LTTLWLGVFGFLDDYLKNFLKLKKGLVPKYKLWGQVSVGLLLTLAIYYGGTGIEVTNL
                     QIPFFKNLIIPLGWTFIPLMVFYITGISNAVNLTDGLDGLSAGVMIFSAVGLGIMSYL
                     KGNFNSAAYLHLEFIPTAGELTVFIAGLIGTLIGFLWFNSHPAEVFMGDTGSLTLGGI
                     LAVISILLKEQIYFVIIGFLFISETLSVIIQRTWFKHTKRRYGEGRRVFLCAPIHHHF
                     EMKGLHESKIVTRFWIVAILLLALGLSTIKLR"
     gene            complement(3839..>5707)
                     /gene="murF"
                     /locus_tag="PLB85_03010"
     CDS             complement(3839..>5707)
                     /gene="murF"
                     /locus_tag="PLB85_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU72327.1"
                     /translation="QIEQALNYVPPVRGRFERVTNKHGIGVFVDYAHTPDALENVLRA
                     SRDLRHERVLCLLGAGGERDHGKRPLMLKAALQFADAVIITDDNPRGEDPDAIVREIV
                     TGTDMWLPWWIIRDRREAIHAILRLARPGDVVLICGKGHETYQEIEGVRHPFDDAEIA
                     AEFMDSEAFGKRAEDELSLPVDRLMLELLCAAKPSENEGYKVPSSYHRVSTDSRTIKP
                     GSVFFALRGPNFDGNAYLGEILRDTSNLGVGSIGTNGWDNYLRAEEPVNAMAMLCRKY
                     LLMFSVYKIALTGSTGKSSTKEFLAAVFSTAAPTLKTLANANNLIGLCQTILNIRPNQ
                     RYAIFELGTNAFGEIAALSDICAPDAGIIINIGPSHLEKLIDERGVYREKTALFNRPL
                     DIRLFDADDPRFEAYSKQGKGVGLSDKADFRLSEVLCDAQACRFKVNGEAYTIAHPVP
                     HMAKNAAFAIAMGRCCGLSETTIKTALAAPVRLEMRLQREDLPRLLLLADCYNANPVS
                     MRSAIEYWHDIEPARPHIAILGDMLELGPAAADYHDMIGAILCDKGYDALYTVGEQAP
                     RYHRQDSHLQNRHFSRVEELLESGVLAGIPEGAVVLVKASHSIHLEKLLPQLRGES"
BASE COUNT         1281 a         1428 c         1733 g         1265 t
ORIGIN      
        1 tgcagttgct gcgcgatggc gatggcgggg aaaatgtgtc cgcccgtgcc gccgcaaccc
       61 aatacaaagc tcaggctgcc atttttccca gtgtttggtg cagtgttcac aagttccgcc
      121 cattctgctt ataacaggca ataaatgaaa tcaaaaggaa gccatcagtc aaatctggca
      181 tcgatacctg aaccgatcca aagctggtcc acttgaaagg acaggagcga cgcaggaggg
      241 atatcacatc tgtctccgtt ttgcagtgag attgagcacc agcccgatcg agatagaatc
      301 cacgagcagg gctgttccgc cgtaactaac gaagggcaac gtgttgccgg tggcggggat
      361 gatggacata ctcacgcctg tattgatgag ggcgttgaga aagatattca tgccgaggcc
      421 tgccatcagg tatttgtgat atcggttttc ctgtgtttcc gccagtttga tgatgcggaa
      481 aaagagtaaa gcgtgcagta ataacaccaa ggcagcgccg aggaagccga attcctcgcc
      541 gatgatggtg tagatatagt cggtgcgggc ttcgggcagg aaatagtgtt tggcgcggcc
      601 gtgggccatg cctgtgccca aaaagcctcc gctggtgaga gcggtgaggc tttccctcac
      661 ctgatattcc tcgggcgaat tgctcggctc ggggcggttg gggataagca gcgaataaac
      721 tttgtaggtg ttgatgcggt cgaagcggaa ggaatctcct ttagtgatga ccacggttcc
      781 ggctatcatg cccagcatca gcattcccag aacgaaacgt ttgcgcaagc ccgcgtagac
      841 gagcatgccc aaaagggtgg cgccgcagat tataagggtg ctgaggtgct tgccaaggaa
      901 gatgagtatg aaaatcaccg ttgtgtacag tatcaacggg atgaactcta taacaagttc
      961 cttcacattg gcgaggtaga tcttgtcctg cttggtatcc agatagccgg caaaatagag
     1021 gatcagcacc aatcgcgcca gcgtgctggg ctgaatgccc acggggccta acatcaacca
     1081 gcgctcggcg ccttttgttt tcgtgccggt gaagatgacc agcaccaaca ggcctatgat
     1141 cagatagacc agccagcggt tgagggggcg cagtttgtcc actttcacga aatagagggt
     1201 gatgatggta gcgaatatgg aaataagcac aaagatggcg tggcggtagt aattaaccat
     1261 tgagctttgc actgaagaga tgtccagcat caccaacagg ccgatcacaa gcagtgcaag
     1321 gtagcatccc aggatggttt tgtcgatgga gacaatgtag gatgtgttcc tattcttttt
     1381 catattcgct cctgattatg tgcacgatct ccttgaaaac ctcgccgcgg tgttcgtagt
     1441 tgcggaaatg gtcaaagctg gcacacgcgg gggagagaac aacgatgtcg cctgggactg
     1501 aatcggcaaa cgcggcacga atgcaggtct caaaatcatc cacgcaatcg agttcgagct
     1561 tgccttccca ggcggcacgc attttttcca tggttccgcc ggttaagtaa accttgcggg
     1621 cgcgctgctt cagcaactcg gtgagaacgg aaaaattctc acccttgtct gaaccgccca
     1681 tgatcacacg gattggctgg tcgaacgaag tgagggctga tctgaccgag tcggtgttcg
     1741 tggccttgga atcgttgtag aagaaaattc cgttgatgat ggcaacaaac tcgaggcggt
     1801 ggctgagagg ctcaaagctt ttggcagcct cgatggcaag ggagatgtcg tgggtgaggg
     1861 catccactgt tagaagcgcc gccatagcgt tggcatggtt gtgggggccg cgtatcttta
     1921 gttcgcttat gggcagtttg tggcgaaggc cgatctggat tgcgtcgcgg ttcagccagg
     1981 cttcgcagtc gggcaggctt ttcgttagcg aatagcgcag caggcgcgat ttgatcaggt
     2041 gctggcgctc cacgacgggt tccgaatcca gacaggtaac ggcggtgtcc gtctgagtct
     2101 gattcgtgaa caagcggaat tttgaggcgg cgtagtcggc aaaggaagca tagcggtcaa
     2161 ggtggtcggg cgtgatgttc aaaagcaccg ccacgtcggg gcgaaagcta tcgatcaggt
     2221 caagctggaa gctgcttacc tcgagcacga tgaaatcaat acccggcttt tcaatgggcc
     2281 agcagcagaa agcggagccg atgtttcctg ccagaatgct gttgtagccc atgtttttga
     2341 ggatgtggtg gatgagcgaa acagtggtgc tcttgccgtt ggaaccgcta acagcgacca
     2401 ccttgctgtc actactcttg acgcggaaac cgagttcgat ctcgctgatg agttctattc
     2461 ccttggcgcg cgctttggcg atgatgggca gattcaaagg tatgccgggg cttacgatcc
     2521 atgtgtcgca ttggaagagg cggtcgctat ggccgccaaa ttcgcagtcg aaatcgctgg
     2581 cgatggtgtc cgcgccgctg atcttctcaa ttggctgggc gtcgctcaga aaggcggttc
     2641 cgcccaattc cctgatcttg tatgccgccg cgatgccgct gcgggccaag ccgagaattg
     2701 cgtatttacg ggaagcgtca aacatttatc acctctcctt agcgcagctt gatagtgctg
     2761 agccccaacg ccagcaagag gatggccacg atccagaagc gggtcacgat tttggattcg
     2821 tgcaagccct tcatctcgaa atggtggtgg ataggcgcgc agaggaacac ccttctgcct
     2881 tctccgtatc tgcgtttggt atgcttgaac cacgtgcgct gaatgattac ggacagggtt
     2941 tcggatatga agaggaagcc aatgatgaca aaatagatct gctctttcag caggatggag
     3001 ataacagcca ggatgccgcc caatgtgagc gatccggtgt cgcccatgaa aacttccgcc
     3061 ggatgggaat tgaaccagag gaaaccgatc agggtgccga taagtcccgc aatgaagacc
     3121 gtcagctctc ccgcggttgg tatgaattca agatgcagat aggcggctga attgaagttt
     3181 cccttcagat agctcatgat gcccaatcca accgcggaaa agatcatcac gcccgctgag
     3241 aggccatcga ggccatcggt gaggttcacg gcgttggaga tgcccgtgat gtagaaaacc
     3301 atcagcggta tgaaagtcca gcccaggggg atgatgaggt ttttgaaaaa aggtatctgg
     3361 agattggtga cctcaatgcc ggtgccgccg taatagatgg caagggttag cagcaatccc
     3421 acgcttacct gcccccacag cttgtatttc ggcaccagcc ccttcttcag cttgaggaag
     3481 tttttcaaat aatcgtccag aaagccgaat acgcccaacc acagcgtggt gagaaacatc
     3541 atgatgatgg agcggttcgt gagatcgttc caaagcagcg aggcgatcag caggccaaag
     3601 atgatgagca gtccgcccat cgttggcgtt ccggccttgg cgcggtgctt ttcgggaacg
     3661 tcagcgtcaa tggtttccac cgcggctttg cgtttcagca atcggatgaa agaaggccct
     3721 gccataaggg tgaaaagcag cgcggtaacg aaagcgccga gggaacggaa ggtgatatag
     3781 cgcatcacgt tgaatacaat gctgtaacgg gaaagggggt agagcagatg gtaaagcatt
     3841 agctttctcc tctcagttgg ggcagcagtt tttcgaggtg gatggaatgc gaggctttca
     3901 ccagcaccac agctccctcg ggaatgccag ccagcacgcc gctttccagc agttcttcaa
     3961 ccctgctgaa atgcctgttc tggaggtgag aatcctgtcg atgatagcgc ggcgcttgct
     4021 ctcccacggt ataaagagcg tcgtaaccct tgtcacagag gatggcgccg atcatgtcgt
     4081 gataatcagc cgcggcagga ccgagttcca gcatgtcgcc caaaatggct atgtgaggac
     4141 gtgctggttc aatatcatgc caatattcta tggcgcttcg cattgagacg gggtttgcgt
     4201 tgtagcaatc cgccaaaagc agcaggcggg gaaggtcttc gcgctgcaaa cgcatttcca
     4261 gtctgacggg ggccgccaaa gccgttttga tcgttgtctc cgatagtccg cagcatctgc
     4321 ccatggcgat ggcgaatgcg gcgtttttgg ccatgtgcgg cacaggatgg gcgattgtgt
     4381 aggcttcgcc attcactttg aagcgacagg cctgagcgtc gcaaagcacc tcgctgaggc
     4441 ggaaatccgc cttatcagag aggccaacgc cttttccctg tttagaataa gcctcgaagc
     4501 ggggatcgtc ggcatcgaag agccggatgt ccagaggacg gttgaagagc gccgttttct
     4561 cgcgatagac gcccctctcg tcgatgagct tttccaggtg cgaagggccg atgttgataa
     4621 tgatgccggc gtctggagcg cagatgtcgc tgagggcggc aatttcgccg aaagcgttgg
     4681 tgccgagctc gaagatggca tagcgctggt tcgggcggat attgaggatg gtctggcaaa
     4741 ggcctatgag gttattggca ttggctaggg tcttcagcgt tggcgcggcg gtgctgaaaa
     4801 cggctgccag aaactctttg gtgctgcttt tgccggtgct gccggtcaac gctatcttat
     4861 aaacggaaaa catcaacaga tacttgcggc aaagcatggc cattgcgttc acaggctctt
     4921 ccgcgcgcag gtagttgtcc cagccgttgg tgccgatgct gcccacgccg aggttgctgg
     4981 tgtcgcgcag gatttcgccc agataggcgt ttccgtcgaa atttggcccg cgcagggcga
     5041 agaataccga tcccggcttg atggtacggc tgtcggtgga aaccctgtga taggaggagg
     5101 gcactttgta gccttcgttt tcggagggtt tcgcggcgca gagcagttca agcatgaggc
     5161 ggtcgacggg caggctgagt tcgtcctccg cccgcttgcc gaaagcttcc gaatccatga
     5221 attctgcggc gatttcggca tcgtcgaagg gatgccgcac gccctctatc tcctgatagg
     5281 tctcgtgtcc ctttccgcaa atcaaaacga cgtcgccggg ccgggccagg cgcaaaatgg
     5341 cgtgaattgc ttcacggcgg tcgcggataa tccaccaggg cagccacatg tccgtgccag
     5401 taacgatttc gcggacgatc gcatcggggt cttcgccgcg ggggttgtcg tcggtgatga
     5461 tgacggcgtc ggcgaattgc agagcggctt tcagcatcag cggacgcttg ccgtggtcac
     5521 gttcgccgcc agcgccgagg aggcagagaa cgcgttcgtg gcgcaggtca cggctggcgc
     5581 ggagcacatt ttccagggcg tcgggcgtat gcgcgtaatc gacgaaaacg ccgatgccgt
     5641 gcttgtttgt gacccgttca aagcgtcccc gcacgggcgg aacatagttc agcgcctgct
     5701 ctatctg
//
LOCUS       DAOOWR010000081         5679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14367_length_5679_cov_4.514047, whole genome shotgun sequence.
ACCESSION   DAOOWR010000081 DAOOWR010000000
VERSION     DAOOWR010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_5679_cov_4.514047"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLB85_03015"
     CDS             complement(<1..440)
                     /locus_tag="PLB85_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72328.1"
                     /translation="MKQILTRYLSELQKVINRGDAREESFYHCVKEMLESYAQANGFR
                     RSEVSILPKATEAGNPDFRVWDGKTRITGYIEAKKPEVSQLESISESEQLKRYRDTFD
                     NLILTNFYEFWLFQYGKFVCGVTIADPLEAIHSQKKPPLTNIED"
     gene            853..1302
                     /locus_tag="PLB85_03020"
     CDS             853..1302
                     /locus_tag="PLB85_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HOU72329.1"
                     /translation="MSGPFNGYWENAVHNQRVIIPALFKRRFSEESQRTVVITAGFDN
                     SIALYPLDNWYSTLAELAEGNEMEQDFRSMLIRCAVAEAELEGPGRVRLPERELRKAG
                     ISDRCVVKGDHHLISLWNPERFYTDLENYEAEIRSRFDAKLWHKGRK"
     gene            1299..2240
                     /gene="rsmH"
                     /locus_tag="PLB85_03025"
     CDS             1299..2240
                     /gene="rsmH"
                     /locus_tag="PLB85_03025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU72330.1"
                     /translation="MNTYHTPVMLAECVAWLQPRAGGVYVDATLGGGGHTLGMLKAAK
                     GIMVYGFDQDADALAEAKERLSEYGNSCELIGTNFSRLRTELALRKVKSIDGALFDLG
                     VSSHQLDEQSRGFSFDGDAPLDMRMDRGQAYTAADAVNTLTQAELTRIFKEYGEELNA
                     GRIAAKIVKERVKQPLQTTADLARVVESVAGKGSKESLKSKARIFQALRIHVNRELDV
                     LAPALQDAIDLLAPGGRIVVLSYHSLEDRIVKNIFKTAADGCVCPPKAMSCNCGKHKQ
                     LALLNKKPLVPSDEETDSNSRSRSVKLRAAEKIMGEK"
     gene            2237..2587
                     /locus_tag="PLB85_03030"
     CDS             2237..2587
                     /locus_tag="PLB85_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72331.1"
                     /translation="MRAKFVILILIAVVAVFANFWNANQMVRATQKLARMETKLAAEK
                     NINTELRVESDDLRSGRQFAELMSADANGQPVQAGKIIYVHEPADKQSRESYCIIDLF
                     ASKAQAKEVQILLD"
     gene            2594..4768
                     /locus_tag="PLB85_03035"
     CDS             2594..4768
                     /locus_tag="PLB85_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU72332.1"
                     /translation="MRSRYKLLAVCFGLLTLLWVGYLFALQIFDPFKLDQQRLTRYTP
                     HKEIIIPTRGSIYDANGNLLVSSISYYQLDIDRKAVKLWADEKKLELSEAYRLISNAI
                     GNNCSMKPDEVMRRLTMNEKLTPIQITNKVREMELEKVVKAFETDNLPGLNHSFASMK
                     RIYSRNILAARLLGSVRAVSEGFDAETGNRSLYKLSGICGIESSYDEYLAGEYGWREV
                     VYDAKHRRMPYPNLHEKVSRDGYNLHLTIDANIQEVVENALYEGLEKYKAKNGGAIVM
                     DPHTGRILAMAGVSAEDRYIDPGLVRVKSNIPVSFMFEPGSTMKPLTMLVALDKHVVS
                     HKDLFPSGSITIRGRTISDTHSYGMIRPVDIICKSSNVGIALIGDKIGAKTLYEKFIA
                     LGYGQKTGLDLSGESSGMFAKLKNWDGYTLHSVSFGQAISVTAIQHATAFCAVANGGR
                     MVKPYLLESVTDNNGRIIEQFEPQVLRQVAGKAAADTVRYYMQTVVDRGTARHIKMDY
                     ITMGGKTGTAQKAAEGGGGYAGGKYNAVFVGLFPVEAPKMVMIVFYDEPAIGYHYGST
                     SAAPTFKQIVEDILFMPNCNILAFDERVMQSSKRMPDLRGKHISQAEALLTQFGFSYN
                     IVGADSASVVIDQFPKPNVAIDPGHPITVKIGRKAGEAAETSVRGSMPDLRGLTLRKA
                     MQVAARVKVALRVRGSGIVRAQSIQPGSRVTNNTVCTLEASI"
     gene            4765..>5679
                     /locus_tag="PLB85_03040"
     CDS             4765..>5679
                     /locus_tag="PLB85_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOU72333.1"
                     /translation="MRLSKVLQLLKQHDLLLQSQALEGIDSLSGAVRVDNREIEAGDI
                     FVCIKGQVADGHRFIPDARARGASLIVCEDEFTDALPAIRVSDSRKATALLAKLFYND
                     PSSSFRLVGVTGTNGKTTTSMLLFKATRELGISAGWIGTLGYYINDNHYETRHTTPDI
                     LQLNGIFAQMAEQGLKLVFMEVSSHALALDRVFGIEFDYCLFTNLSREHLDFHHNMEE
                     YGKAKLKLFAGTLSGKAVGLINTDDSFGKRIYGELKAEGAYVFSLGSENADFVIRGDG
                     SHNDWEQSRFTLQCREGMVNIRSRLIGKF"
BASE COUNT         1393 a         1608 c         1594 g         1084 t
ORIGIN      
        1 aaatcttcta tgtttgtgag gggcggtttt ttttgggaat ggatggcttc gagcggatcg
       61 gcgatggtta cgccacagac gaattttcca tattgaaaca gccaaaactc gtagaaattg
      121 gtgaggatga gattgtcgaa agtatccctg taacgcttca actgttcgct ttcggagatg
      181 gattcgagct ggctgacctc cggttttttg gcctcaatgt agcccgtgat gcgggttttc
      241 ccgtcccaca cgcgaaaatc gggattccca gcttcggtgg ccttggggag aatgcttacc
      301 tcactgcgcc tgaaaccgtt ggcttgggcg tagctttcca gcatttcctt cacgcagtgg
      361 tagaaggatt cttccctggc atcgccgcgg ttgataactt tctgcagttc gctcagatat
      421 ctggtcagta tttgtttcaa cagctctcct cttgataagg gcatatttac ttggaaagca
      481 ctctttgggg ggaggtaaat ttagtcaatt actttttgga tcagggcagg ggttggtggt
      541 attggcgtct gacatttcag tctctgcctc cgccagagaa tatccgattg tccattccgg
      601 cagagctttg tccggctctc agccagcctg tttctcccga ctttccgcag acaaaaacgc
      661 ccccgcgcgc ggtgttaagg acaaaaaaat tcaaaaaagc gcagattctg cttgacaaga
      721 aaacgcgatg tcatataagc ggcacaggat gtcaaaaaaa gtcatacagt gtcagagaga
      781 atcaaacaag atgttgctgc aagtcaaatc aagagcggtt tggcggctaa agagagagaa
      841 aaggaaactt agatgtcagg accattcaac ggttattggg aaaacgcggt tcacaaccaa
      901 cgggtgatca tcccagccct gttcaagcgc agattcagcg aagaatccca gcgcacggtg
      961 gtgatcacgg ccggtttcga caattccatc gccctctatc cattggacaa ttggtattcc
     1021 acgctggcag agctggcgga aggaaacgaa atggagcagg atttccgctc gatgctgatc
     1081 cgctgcgccg tggcggaagc ggaactggaa ggccccggcc gggtgaggct gccggaacgc
     1141 gaactgcgca aagcgggcat cagcgaccgc tgcgtggtga aaggcgacca ccacctcatc
     1201 tctctgtgga atcctgaacg cttctacacc gaccttgaaa actacgaggc ggaaatccgc
     1261 agcagattcg acgccaaact ctggcacaaa ggcagaaaat gaatacttac cacacccccg
     1321 tgatgctggc tgaatgcgtt gcctggctgc aaccgcgggc cggcggggtg tatgtggacg
     1381 ccactttggg cggcggcggg cacactttgg gaatgctgaa agcggcgaaa gggataatgg
     1441 tttacggttt tgaccaggac gccgacgcgc tggcggaggc gaaggaacgg ctgagcgaat
     1501 acggaaatag ctgcgaactg atcggcacga atttttccag gctgcgcacg gaactcgccc
     1561 tgcgcaaagt gaaaagcatc gacggcgcgc tttttgacct gggcgtgtcg tcacaccaac
     1621 tggatgagca gtcacgcggc ttcagcttcg acggcgacgc gcctttggac atgcgcatgg
     1681 accgggggca ggcctacacc gccgccgacg ccgtgaacac gctcacgcag gccgaactga
     1741 ccaggatttt caaagaatac ggcgaagagc tgaatgcagg aaggatcgcg gcaaaaatcg
     1801 tgaaggaacg cgtcaagcag cctctgcaaa caacagccga ccttgcccgc gtcgtcgaaa
     1861 gcgtggcggg caagggcagc aaggaatcgc tgaagagcaa agcgcgcatc ttccaggctt
     1921 tgcgcatcca cgtgaaccgc gaactcgacg tgctggcccc ggctttgcag gacgccatcg
     1981 acctgctcgc cccgggcggc agaattgtcg tgctcagcta ccattcgctg gaagacagga
     2041 tagtaaagaa cattttcaaa accgccgccg acggctgcgt ctgccctccc aaggcgatga
     2101 gctgcaactg cggcaaacac aaacagctgg ccctgctgaa caaaaagccg cttgtgccca
     2161 gcgatgaaga gaccgattca aacagccgct cccgcagcgt gaaactgcgc gcggcggaaa
     2221 aaatcatggg ggaaaaatga gagccaaatt cgtgattttg atcttgatag ccgtcgtggc
     2281 cgtatttgcc aatttttgga acgccaacca gatggtgcgc gccacgcaga aactggcgcg
     2341 gatggaaacc aagctcgcgg cggaaaagaa catcaacacc gaactcaggg tggaaagcga
     2401 cgacctgcgc agcggcagac aattcgccga gctgatgagc gctgacgcca acggacagcc
     2461 ggttcaggcc ggaaagatca tctacgttca cgaacccgcg gataaacaaa gccgggaaag
     2521 ctattgcatc attgacctct tcgcttccaa agcgcaggcg aaggaagttc agatcctgct
     2581 ggactagttg gcgatgcgtt cccgctacaa actgctggcg gtatgttttg gcctgctgac
     2641 cctcctgtgg gtgggctacc tcttcgcgct gcagatattc gaccccttca agcttgacca
     2701 acagcgcctt acgcgctata ccccccataa agagatcatc atccccacgc ggggctccat
     2761 ttacgacgcc aacggcaatc tgctggtgag ttccatcagc tattaccaac tggatataga
     2821 ccgcaaagcg gtcaagcttt gggcagatga gaaaaagctt gaactaagcg aagcttaccg
     2881 attgataagc aatgcaatag gcaacaactg cagcatgaag cccgacgaag tgatgcggcg
     2941 gctgacgatg aacgaaaagc tgacccccat ccaaatcacc aacaaggtgc gcgagatgga
     3001 gctggaaaaa gtcgttaaag cctttgaaac cgacaatctg ccggggctca accacagctt
     3061 cgcctcgatg aagcgcatct attcgcgcaa cattctggca gcaaggcttt tgggcagcgt
     3121 tcgcgccgtc tccgaaggct tcgacgccga aacgggcaac cgctcgctct acaaattaag
     3181 cggaatctgc ggaatcgaat ccagctacga cgaatacctg gccggagaat acggctggcg
     3241 ggaagtggtt tacgacgcca aacaccggcg catgccatat ccgaatttgc acgaaaaggt
     3301 ttcccgcgac ggctataacc tgcacctcac catcgacgcc aatatccagg aagtggtgga
     3361 gaacgcgctc tatgaaggcc tcgagaaata caaggccaaa aacggcggcg cgatcgtcat
     3421 ggacccccac acgggccgca tcctggcgat ggctggcgtt tccgccgaag acagatacat
     3481 cgacccgggg ctggtgaggg ttaaatccaa tataccggtg agcttcatgt tcgagccggg
     3541 ctccacgatg aagccgctca cgatgctggt ggcgctcgac aagcacgtcg tgagccacaa
     3601 agacctcttc cccagcggct ccatcaccat ccgcggccgc accatctctg acacccacag
     3661 ctacggcatg atccgccccg tggacatcat ctgcaaatcc agcaacgtgg gcatcgcgct
     3721 gatcggcgac aaaatcggcg ccaaaacgct ttacgagaaa ttcatcgccc tcggttacgg
     3781 gcagaaaacg gggctggacc tgagcggcga aagcagcgga atgtttgcca aactgaagaa
     3841 ctgggacggc tacacgctgc actcggtatc ctttgggcag gccatttccg tgaccgccat
     3901 ccagcacgcg acggcctttt gcgctgttgc caacggcggc aggatggtga agccctatct
     3961 gctggaatcc gttaccgaca acaacggcag gataatcgaa caattcgagc cgcaggtgct
     4021 gcgccaggtg gcggggaaag ccgccgcgga cacggttcgc tactacatgc aaacggtggt
     4081 tgaccgcggc acggcgcgcc acatcaagat ggattacatc accatgggcg gaaagacagg
     4141 caccgcgcag aaagcggctg aaggcggcgg aggctatgcc ggcggcaaat acaacgccgt
     4201 atttgtgggc ttgttccccg tggaagcgcc caaaatggtg atgatagttt tctacgacga
     4261 gcccgccatc ggctaccatt acggcagcac aagcgccgcg cccactttca agcagatcgt
     4321 ggaagacatc cttttcatgc cgaactgcaa catcctggcc ttcgacgagc gggtgatgca
     4381 aagctccaaa cgcatgcccg acctgcgcgg aaagcatatc agccaggcgg aagccctcct
     4441 cacccaattt ggcttttcct ataatatagt aggggcggat tcagcctccg tggtgatcga
     4501 tcagttcccc aagccgaacg tcgccatcga tccggggcat cccataaccg tgaagatagg
     4561 ccgcaaagcc ggcgaggctg ctgaaacctc cgtccgcggc agcatgcccg acctcagggg
     4621 cctcaccctg cgcaaagcga tgcaggttgc tgcccgcgta aaggttgcgc tacgcgtccg
     4681 tggctctgga atcgtgcgcg cgcaatccat ccaacccggt tcacgcgtca ccaataacac
     4741 agtttgcaca ctcgaggcct caatatgagg ctgagcaagg ttttacagct cttaaagcag
     4801 cacgatctgc tgctgcaaag ccaggccctc gaaggcattg acagcctgtc cggcgcggtg
     4861 cgcgtggaca acagggaaat cgaagcgggc gacatctttg tctgcatcaa aggtcaggtc
     4921 gccgatggac accgcttcat ccctgacgcc cgagccagag gggcctccct catcgtttgc
     4981 gaagacgaat tcacggacgc cctgccggcc atccgcgtga gtgattcgcg caaggcgaca
     5041 gcgttgctgg caaaactttt ttacaacgac cccagctctt ccttccgcct cgttggagtg
     5101 acgggaacca acggcaagac aaccacttcc atgctgctat ttaaagcgac gcgtgaactc
     5161 ggcatcagcg ccggctggat cggaacgctg ggctattaca tcaatgacaa ccactacgaa
     5221 acgcggcaca caacgcccga catcctgcag ctcaacggca tttttgcgca gatggcggag
     5281 caggggctga agcttgtttt catggaggta agctcccacg cgctggctct ggaccgcgtt
     5341 ttcggcatcg aatttgacta ttgcctcttc accaacctca gccgcgaaca cctggacttc
     5401 catcacaaca tggaggaata cggcaaagcc aagctgaagc tgttcgcggg aacgctcagt
     5461 ggcaaagctg tgggcctgat caacaccgat gacagctttg gcaaacgcat ttacggtgaa
     5521 ctgaaggcgg aaggtgccta cgtgttcagc ctcggcagtg aaaacgcaga cttcgtgatc
     5581 cgcggcgacg gcagccacaa tgattgggaa cagagccgct tcacgctgca atgcagggag
     5641 ggaatggtga acatccgctc gcgcctgatc ggcaaattc
//
LOCUS       DAOOWR010000082         5667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14414_length_5667_cov_3.758731, whole genome shotgun sequence.
ACCESSION   DAOOWR010000082 DAOOWR010000000
VERSION     DAOOWR010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14414_length_5667_cov_3.758731"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLB85_03045"
     CDS             <1..197
                     /locus_tag="PLB85_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72334.1"
                     /translation="EHCEVRLFNIKGQELYSGRANRHHKGQGFELPKEAFDRLPAGIY
                     LISLNPGNGKTATARLVVVE"
     gene            complement(453..998)
                     /locus_tag="PLB85_03050"
     CDS             complement(453..998)
                     /locus_tag="PLB85_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU72335.1"
                     /translation="MMTTEMICAGFGGQGVLTIGRFIAQAGMKEGKNVSWLPSYGPEM
                     RGGTANVSTVVSDGPIASPIVSYPDVLVALNQPSLDKFGPSVRSGGVVIINTNSCPHG
                     CKRDDVQIVAAPMTDISLEIGSMRVMNMLAIGIVIGKTGLIKYETMEEDLTSFLKAKD
                     PDLLEKNLAAIKRGMEIGKQQ"
     gene            complement(995..1744)
                     /locus_tag="PLB85_03055"
     CDS             complement(995..1744)
                     /locus_tag="PLB85_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU72336.1"
                     /translation="MQVIAKRPETLTKTPFTYCPGCLHGVAHRLIAEAIEEQGLINLM
                     TGVAPVGCSVFAYKFFNFDMAEAAHGRAPAVATGVKRARPDMHVFTYQGDGDLAAIGT
                     AEIVHAANRGEHISVFFVNNAIYGMTGGQMAPTTLPEMKTTTSPYGRKVDDIGYPIRV
                     CELLATLVAPYYIERVSLLSPAEIIKAKKAVDKAIRYNKEGRGFTFIEFISTCPTNWG
                     MDPVKSRDWARENMLPFFKPGLFRDKGEGVE"
     gene            complement(1759..2838)
                     /locus_tag="PLB85_03060"
     CDS             complement(1759..2838)
                     /locus_tag="PLB85_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU72337.1"
                     /translation="MSKILMKGNEAVAEAAIRSGCRLYFAYPITPQSELIEYMAKMMP
                     KVGGTFLQAESEVSAINMVYGAAGAGKRVMTSSSSPGISLKMEGLSYIAGAELPAVII
                     NVQRGGPGLGDIQPAQGDYFQATKGGGHGDYRLIVLAPSSVQEFADMASEAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKEAKTQEEIEALGSQHLSWCICPNEDGDKKHHHEINS
                     LEIDPAVLEKHVEKLYEKYAEIEKNEIKYDTYNLSDDNEVLCVAWGTASRIVKSAINE
                     LTAAGKSVGLIRPITCWPYPYEAVAAAIGPKVKEVYVFELNTGQMLDDVKIAVAGKVP
                     VKFWGKVGGIVFTPAEIQAKLEACF"
     gene            complement(2846..3040)
                     /locus_tag="PLB85_03065"
     CDS             complement(2846..3040)
                     /locus_tag="PLB85_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOU72338.1"
                     /translation="MTVDPFYCKGCGLCIAACPKKIIRFSEDINAKGYHYAECFKQDE
                     CIACKMCYMTCPDVAITVEK"
     gene            3349..3849
                     /locus_tag="PLB85_03070"
     CDS             3349..3849
                     /locus_tag="PLB85_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOU72339.1"
                     /translation="MRNQSKKTLSTVFAIWVMFIAPILAQTVVGQPSTEELTIPYEQV
                     AVIDSLQNQEPPSVEEAPPGETWVASYYGKRFHGRRTASGEVYNQNAMTCAHKTLPFQ
                     TLLKITNPKNGKNVIVRVTDRGPFKHGRDIDLSYGAAKEIGMLAAGVMKVEVVQLQDR
                     KEYTEL"
     gene            3939..4859
                     /locus_tag="PLB85_03075"
     CDS             3939..4859
                     /locus_tag="PLB85_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOU72340.1"
                     /translation="MTELIKKLALSPEVSSALAKRRPVVALESTVLTHGLPWPQNLAV
                     FRKLEEIIVNEDVTPATIIVLDGTAHIGVTENLIEDITPSLKKPGTFLKLGSRDLPLA
                     LSLGHSGGTTVSATMKLASLAGIKVFATGGIGGVHRGWEKVPDVSSDLTALASIPVTV
                     VSSGCKAILDVPATLEYLETLAVPVLGWQTDSFPLFYTAESELRIDRIDSVEGFSLLW
                     KNHLELGGKGLLIANPIPAADSIPAPQMEAFINQALVAARLHGITGKALTPFLLDYLA
                     QATKGGSVTANLALLRNNAKVASELAKAIV"
     gene            4872..>5667
                     /locus_tag="PLB85_03080"
     CDS             4872..>5667
                     /locus_tag="PLB85_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOU72341.1"
                     /translation="MRIYISIDMEGMPGTFNWEHEKTDRPAVQKCITQHVTMALLAAL
                     GSPHAAMIEEITIADSHSGGDNLDYSITELDARINLISGCPRPRYMMPELSKDYSQVW
                     LLGYHSGTGALRGNMDHTYSNSRIHNIWINGQRMNEALINSAFAGYKQIPVTLVTGDE
                     ALKTELAAPMPWLEYVATKQAVAKFSAKNYSRLAVDKRTRATVLNALSKSPDSLPLYR
                     FEAPITLKIEFNHSSMADQAALMPYTKRLDGRTLEFTADDYEIVFET"
BASE COUNT         1299 a         1587 c         1519 g         1262 t
ORIGIN      
        1 gcgaacattg tgaggtcagg ctgttcaaca tcaagggaca ggagctgtat tccggcaggg
       61 ccaaccggca tcacaaaggg caaggctttg agcttcccaa agaggctttt gaccgtttgc
      121 ccgccgggat ttaccttatc agcctgaatc cgggaaacgg caaaaccgcc actgccaggc
      181 tggtggtggt tgaataaaac ttcctataaa atcagagggc ccggcatcca gcgagcccgc
      241 gagcttggac tacaggagtt gctgctgtcg tcgatgctcg ttcctcgctc gacgccagcc
      301 gggtagctct gaagatcaga actgtccgtc attccagcgc ttccacccat tcaaattgaa
      361 cgtgagtcaa aaaaagacgc ggatggattc cgcgtcttct gatatcattt gcctgcttca
      421 ctccttacaa aggggcataa gccggacgga tgctattgct gtttgcctat ctccattccg
      481 cgtttgatgg ccgcgaggtt cttttccagc aaatctgggt ctttggcttt caggaaggaa
      541 gtgaggtctt cctccatggt ttcgtatttg atgagccctg ttttgccgat cacaatgccg
      601 atggccagca tgttcataac ccgcatggag ccgatttcca gagagatgtc ggtcatgggg
      661 gcggccacga tctgaacgtc gtcccgtttg cagccgtggg ggcaggagtt ggtgttgatg
      721 atcaccaccc cgccggagcg gacactgggt ccgaatttat ccagggaagg ctggttcagg
      781 gccaccaaca catcggggta gctcacgatg ggagaagcga tggggccgtc ggaaacaacg
      841 gtggagacgt tggcggttcc gccgcgcatt tcggggccgt aggaaggcag ccaggagacg
      901 ttctttcctt ctttcatacc cgcctgggcg atgaatctgc caatggtgag aacgccttgt
      961 ccgccgaatc ccgcgcaaat catttcagta gtcatcattc caccccctcg cccttgtccc
     1021 ggaacaagcc gggcttgaag aagggcagca tgttttcccg cgcccaatcg cgcgatttga
     1081 cagggtccat accccagttg gtggggcagg tgcttatgaa ttcgataaag gtaaagccgc
     1141 ggccttcttt gttatagcgg atggctttgt ccacggcctt tttggcttta ataatctcgg
     1201 ccgggctgag cagcgacacc ctttcaatat aatagggcgc caccagggtg gccagaagtt
     1261 cgcaaacgcg gatgggatag ccgatatcgt ccactttgcg gccatagggg ctggtggttg
     1321 tcttcatctc gggcagggtg gtgggggcca tctggcctcc cgtcatgccg tagatggcgt
     1381 tgttcacaaa gaaaacgctg atgtgctcgc cacgattcgc ggcgtgaact atctcggcgg
     1441 tgccgatggc ggcaaggtcg ccatcgccct gataggtaaa gacatgcatg tccggccggg
     1501 cacgtttcac accggtggcg acagccgggg cgcgtccgtg ggcggcttca gccatgtcga
     1561 aattaaagaa tttataggcg aaaacagagc agccaacagg ggccaccccg gtcattaaat
     1621 tgatcagccc ctgctcttcg atcgcttcag cgatgaggcg gtgggccact ccgtggaggc
     1681 agccggggca ataggtgaag ggtgttttgg tgagagtttc aggtcttttg gcgattactt
     1741 gcatttcttt cctccttctt agaaacaggc ttccagtttg gcctggatct ccgccggggt
     1801 gaacacaatc ccgccaacct ttccccagaa tttaaccggg accttgccgg ccacggcgat
     1861 ctttacatcg tccagcattt ggccggtgtt gagttcaaac acataaactt ccttcacctt
     1921 ggggccgatg gcggcggcca cagcctcgta aggataaggc cagcaggtga tgggacggat
     1981 gaggccaacg ctcttgccgg cggctgtgag ttcgttgatg gcggatttca caatgcggga
     2041 agcggttccc caagccacgc agagcacctc gttgtcatcg gagaggttgt aggtatcata
     2101 cttgatttcg tttttctcga tctcagcgta cttttcatag agtttttcca catgtttttc
     2161 cagcacggcc ggatcgatct ccagggagtt tatctcgtgg tggtgtttct tgtcaccgtc
     2221 ttcgttggga cagatgcacc aggaaaggtg ttggctgcca agggcttcga tctcttcctg
     2281 ggtcttcgct tccttgaatt ccaccggctc catcatctgg cccagcatac cgtcggccag
     2341 gatcatcaca ggattgcggt atttgtctgc caaatcgaag gcctcggatg ccatgtcagc
     2401 aaattcctgc acggaactgg gagcgagcac gatgaggcgg taatctccat gtccgccgcc
     2461 cttggtggct tgaaaatagt cgccctgggc aggctggatg tcgcccaggc cgggtccgcc
     2521 gcgctgaacg ttgatgatca ccgctggcag ctccgcgccg gcaatgtagg aaagcccttc
     2581 cattttaagg gaaatccccg gcgaagagga agaggtcatc acgcgttttc cggctcctgc
     2641 ggcgccgtaa accatattga tggccgagac ttcgctttca gcctggagga aggttccccc
     2701 aaccttgggc atcatcttgg ccatgtattc gataagctcg ctctgaggcg tgatcgggta
     2761 ggcgaaatag aggcggcagc cggatctgat cgcggcttcg gccacggctt cgtttccttt
     2821 catcaatatt ttcgacatct caacctcact tctcgaccgt gattgccaca tcggggcagg
     2881 tcatataaca cattttgcag gctatgcatt catcctgctt gaaacactct gcgtagtggt
     2941 atcctttcgc gttaatgtcc tctgaaaacc ggatgatctt cttgggacag gcagcgatac
     3001 agagtccaca acctttgcag taaaacgggt caaccgtcat tttgggcatg gtcgtactcc
     3061 ttttctattg taataattcg tttctgtgat atagcaggca acaggggatt tctgtcaaac
     3121 tatttcttcc gtcgccctcc cggcaggggt ttatgaggcc aaattcgata atccagctaa
     3181 aagggatccc atcccgaagt tttcaaggcc gcggaagccc gattggccgc caaggccaac
     3241 atatagcggc acaagcagtt aagcaggaaa aacgaaaaat aaacttgaca aaaattggcg
     3301 gctcaaaaag ttcagcggcg agacttaagc aaaacatgga aaaccaatat gagaaatcaa
     3361 tctaagaaaa ctctctccac tgttttcgct atctgggtga tgttcatcgc tccgatttta
     3421 gcgcaaacag tggtgggaca accgagcacg gaagagctca caatccctta tgaacaggtg
     3481 gcggtgatcg attccctgca gaaccaagaa cctccaagcg tggaggaagc tccaccgggc
     3541 gagacctggg tagcttccta ctacggtaaa cgcttccacg gtcgacgcac agccagcggt
     3601 gaagtttaca atcaaaacgc tatgacctgc gcacacaaaa ccctcccctt ccaaaccctc
     3661 cttaaaatta ccaatcccaa aaacggcaag aacgtcatag ttcgtgttac cgaccgtggc
     3721 cctttcaaac acggacggga tatagacctc tcctacggcg cggcaaagga aatcggcatg
     3781 ctcgcggctg gcgtgatgaa ggtggaagta gtgcaacttc aggacaggaa agaatacacc
     3841 gagctttagc tcaacccatc ctgaaacaaa acacggaatc aatccttgcc cttatcccag
     3901 agtaacgatt gattccattt ttgtttgtgg aagaatctat gactgaattg ataaaaaagc
     3961 tggccctgtc tcccgaggta agttcagcgc ttgccaaacg ccggccggtg gtggcactgg
     4021 aatccacggt gctcacgcac ggcctgccct ggccgcaaaa cctggctgtg ttccggaaat
     4081 tggaagaaat catcgtgaac gaggatgtga caccagccac gataatcgtt ttggacggaa
     4141 cagcgcatat tggagtgact gagaacctca ttgaggatat caccccgtcg ctgaaaaagc
     4201 cgggaacatt cctaaaactg ggctcgcggg acctgcctct ggccctcagc ctcggccaca
     4261 gcggagggac cacggtttcc gccaccatga agctggcatc tctggctggg atcaaggttt
     4321 tcgccaccgg cgggatcggc ggtgttcacc gcggttggga aaaggtcccg gacgtttcca
     4381 gcgacctgac cgccctcgcg tctattcctg tgacggtggt ttcctccggc tgcaaggcca
     4441 ttttggacgt tcccgcaacc ctggagtatc tggaaaccct ggccgtgcct gttctgggct
     4501 ggcaaactga cagctttccc ctcttctaca ccgcagaaag cgaactccgc atcgaccgga
     4561 ttgacagcgt ggagggtttc tccctgctgt ggaaaaacca tctggaactg ggcgggaaag
     4621 gcctgctgat cgccaatccc atccctgccg cggacagcat tccggccccg caaatggaag
     4681 ctttcataaa tcaggctttg gtcgccgccc gactgcacgg aatcaccgga aaagcgctca
     4741 cgccttttct gcttgactat ctggcgcagg ctactaaagg tggctccgta actgccaatc
     4801 tggcgctgct gcgcaacaac gcgaaagtgg cgtctgaact ggccaaggca atcgtttaag
     4861 aggaacaagc catgagaatt tacatctcga tcgacatgga aggcatgccc ggaactttca
     4921 attgggaaca tgaaaagacg gaccgtccgg ccgtgcagaa atgcattacc cagcatgtga
     4981 ccatggctct gttagccgcc ttgggcagtc cccacgccgc catgatcgaa gagatcacca
     5041 ttgccgactc ccacagcggg ggcgataacc ttgattacag catcacagag cttgatgcca
     5101 ggatcaacct tatttccggc tgtccccggc cccgctacat gatgccggaa ctcagcaagg
     5161 attacagcca ggtctggctg ctcggctacc acagcggcac cggcgccttg cgtggaaaca
     5221 tggaccacac ctattcaaac agccgcatcc acaatatctg gatcaatggc cagagaatga
     5281 acgaagccct catcaattct gccttcgcgg gttataaaca aatccccgtc acccttgtca
     5341 ccggcgacga agccttgaaa accgaactcg cagctcccat gccctggctg gaatatgtgg
     5401 ccaccaagca agccgtggcc aagttttcag ccaaaaacta ctcccggcta gccgtggaca
     5461 aaagaacccg cgcaaccgtt ttgaatgccc tgtccaaaag cccggacagt ctgccgctgt
     5521 ataggtttga agcccccatc accctcaaaa tcgagttcaa ccacagctca atggccgacc
     5581 aggccgccct gatgccctac accaaacgcc ttgacggacg caccctcgaa ttcacggctg
     5641 acgactatga gatagttttc gagacca
//
LOCUS       DAOOWR010000083         5634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14520_length_5634_cov_3.296290, whole genome shotgun sequence.
ACCESSION   DAOOWR010000083 DAOOWR010000000
VERSION     DAOOWR010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_5634_cov_3.296290"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLB85_03085"
     CDS             complement(<1..684)
                     /locus_tag="PLB85_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72342.1"
                     /translation="MPGINLKKNILLGGSSRLLIMGLSFLCSWISARFLGVELKGQYS
                     YLVTLGGFIWMALDLGLYRSYPYLIRKNPDKLNSLLGWTILSFVLETLLLAVLGLGLL
                     RFWNSILGFVFDPLSMLLFIGFITLTKLFMHLQSLYMGMDRIGLHSLGHILNSLTCLL
                     LFALGYFMFQGERRLAFILGSMLLGLTVASLFFLLAKRWGNPWRNWQPRFVLNSYKFG
                     IRVFISSLLI"
     gene            902..2050
                     /locus_tag="PLB85_03090"
     CDS             902..2050
                     /locus_tag="PLB85_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU72343.1"
                     /translation="MIFTQTLFWFSVAALAYHLAGYPLIIAILTRFRKRYNPTPAETA
                     EFPRVTILCPARNEEDVIEAKITSFLELDYPHDRLNMIVISDDSEDATNEIVSRYQDQ
                     RVELVIQKPRQGKQSAHNLVLPRLDCDYVFSTDANSIFAPDALKLLVARMRSDSRLAL
                     VSGELRLTPGEGGKSGEGLYWRYESWLKKLDSDFHSIIGANGSIFLIRRDLFQRVDPQ
                     SVDDFERVLTVLKNGFLAAYEPQANVFEEETESARQEIARKIRIITPQWFVLERNLAL
                     LNPFRYPATSFQLVSHKLLRWLFFVFVLTGFLASAFLLQIRFYRVIFLLQCLIYGLGV
                     LGLILQARGKRPPFFSLPAYLVGMIWSSLVAFVRYLQRKKTATWNPVR"
     gene            2067..3074
                     /locus_tag="PLB85_03095"
     CDS             2067..3074
                     /locus_tag="PLB85_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU72344.1"
                     /translation="MNILITGSNGFVGSRLMWELDRLGHQVQGIDLSAHCDANPHPKT
                     ILGDIRSLPDLKSASGAFELENNAEIDLIIHCAAAKHDFGIKRGEYFSHNKYGTKTLL
                     EFASQKGIRRLIYLSTVSVFGHPIGAADEDAPYAPDHPYGESKLAGELLCVDWIKKDP
                     LRELIVLRPTVIFGPHNYANMYKLLDTMHRRPWVMVGKGDHVKSVVSLDSVTGMVIFA
                     LGLLQPGFQHFNCVDEPYLTLHELMRLIASQPGFRMPRVNIPIQMAVGIGRIFDLPAK
                     LLGIDFPINSDRMKKFATETNFQAAKIRSAGYVQTTSIASSIAEMCAWYLKNHSQGDK
                     N"
     gene            3071..4099
                     /locus_tag="PLB85_03100"
     CDS             3071..4099
                     /locus_tag="PLB85_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU72345.1"
                     /translation="MKKIKLGLIGCGRISKNHFEAVSQIPEAEFVACADVVEEKARLA
                     AETYGITNWYTDHLEMLDKEKLDAVSICTPSGLHPQIGIDVAKHKVNVITEKPMAINI
                     DGASRLIKACDANQVKLFVVKQNRLNSTMQLLKQAVDKNRFGRIFLAQSNVFWQRPQE
                     YYDAAKWRGTWEFDGGAFMNQASHYVDAIYWLLGNVDSVMAYTSTMARRIEAEDTGCA
                     ILHFRSGIIATLNVTMLTYPKNFEGSITIIGEKGTVKVGGVAVNKIEKWEFEDYDDDD
                     RIALDANYQPPNVYGFGHNPYYRNVVDVLLNKGKPFTDGRDGRKSVEIIQAIYQSAKT
                     GTRVSLPL"
     gene            complement(4150..4410)
                     /locus_tag="PLB85_03105"
     CDS             complement(4150..4410)
                     /locus_tag="PLB85_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72346.1"
                     /translation="MIRTGKIIDIDCQCGFRLFRYFKAGKGRLIKCLISRLSEDRVGL
                     TGAATFSRPVCPACGRELGIVMMIHGEPALKLNQGTIKSVRI"
     gene            5075..>5634
                     /locus_tag="PLB85_03110"
     CDS             5075..>5634
                     /locus_tag="PLB85_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU72347.1"
                     /translation="MRETAIREYNLLLQKYFETRLDMIEASLKQYISPQTAPAKELKK
                     ATRYAIFPGGKRWRPLLLLAMYDMLTGMKGKSMAEALKAATALEIAHNAALTHDDLPF
                     LMNRTERRAAPALHMKYDNTVGILAGDALYALAFEIMSDITPPSKALACIRILSISTR
                     TYGLIGGQAVALESKHRVMKLNTLRY"
BASE COUNT         1415 a         1607 c         1374 g         1238 t
ORIGIN      
        1 gatcagcagg ctggaaatga acacccggat cccgaattta taggaattca atacaaagcg
       61 cggctgccag ttccgccacg gattacccca tcttttggcc agcaagaaaa agagactggc
      121 taccgtcaaa cccagcaaca tcgagcccaa gatgaaggcc agccgccttt ccccctggaa
      181 cataaaataa ccaagggcaa agaggagcaa gcaggttaag gaattgagga tgtgacccag
      241 agagtgcagc ccgattcggt ccatgcccat atagagactc tggaggtgca tgaatagctt
      301 ggtgagggtg atgaagccta tgaaaagcag catggataga ggatcaaaga caaagcccag
      361 aatagagttc cagaaccgca gcaaaccaag tcccaagacc gccaacagca aggtttccag
      421 cacaaaggaa aggatcgtcc agcccagcag gctgttcagc ttgtctgggt ttttgcggat
      481 cagataggga tagctgcggt acaatcccaa atccagcgcc atccagatga aacctcccaa
      541 ggttaccaga taactgtact ggcctttgag ctccaccccc aaaaaacgtg ccgaaatcca
      601 actgcaaagg aaagacaacc ccatgatcag caatctggag ctgccgccaa gcaggatgtt
      661 tttcttcagg ttgattccgg gcataaaatt atatccccgt atcctctgaa gcgtttttca
      721 cgaagttaag actcgacatg ggacacataa ttatccgcag cccgcccctg tcaaatgttt
      781 ctttgcctaa cagacaaata gcttgacaga atgggccttc tcctgacttt gcgtttgtcg
      841 ccttggattc cgacactttc atcaaccagc tggaccagcg acaggaagtg agttaacaaa
      901 catgatcttc acccaaaccc tcttctggtt ctccgtagcc gccttggctt accatctggc
      961 gggatatccc ctcattatcg ccatcctgac ccgtttcagg aaaagataca atcccacccc
     1021 tgcggaaaca gctgaatttc cccgtgtgac catactctgt cccgcccgta acgaggagga
     1081 tgtgatcgag gccaagatta cttctttcct ggaactcgat tacccccatg accgcctgaa
     1141 tatgatcgtc atttcggacg attcagagga cgcgactaat gaaatcgtaa gccgctatca
     1201 ggaccagcgg gtggaactgg tgatccaaaa accgcgtcaa ggcaagcaat cagcccacaa
     1261 cctggtactg ccgcggctgg attgcgatta cgtgttttct actgatgcca actccatttt
     1321 cgcgcctgat gccctgaagc tcttggttgc cagaatgcgc tctgattcca gactggcttt
     1381 ggtttcaggt gaactgaggc tcaccccggg agaaggtgga aaatccggtg aaggcctcta
     1441 ctggcgctac gaatcctggc tcaagaagct ggacagcgat tttcattcca tcatcggcgc
     1501 caacggctcc atctttctca tccgtcgcga cctctttcag agggttgatc cccagtcggt
     1561 ggacgatttc gagcgcgtac taaccgtact taaaaacggc tttctggcgg cttacgagcc
     1621 ccaggccaac gtttttgagg aagaaactga atccgccaga caggaaatcg cccgtaagat
     1681 ccgcatcatc acccctcaat ggtttgtgct ggaacgcaac ctagcgctac tcaacccctt
     1741 ccgctatccc gccactagct tccagctcgt ttcccacaag ctgctgcgct ggctcttttt
     1801 cgtttttgtg ttgacaggat tccttgcctc cgctttcctg ttacaaattc gcttctaccg
     1861 ggtgatattt ttgctccagt gcctgattta cggattgggc gtgcttggct tgatccttca
     1921 agctcgtggc aagcgcccgc cattcttttc cctgccggca tatctggtgg ggatgatctg
     1981 gtcgtcgctg gtagccttcg tccgctatct gcagagaaag aagaccgcca cttggaaccc
     2041 ggtgcgttag gagacgccct acataaatga atatactaat aacaggcagc aacggctttg
     2101 taggctcccg actgatgtgg gaactcgacc gcctgggcca tcaggttcag gggatagacc
     2161 tcagcgccca ctgcgacgcc aaccctcatc ccaagacgat cctgggtgat atccgctccc
     2221 tgcccgacct caaatccgcc tccggagctt tcgagctgga aaacaacgcc gagatcgatc
     2281 ttatcattca ctgcgccgct gccaaacacg atttcggcat caagcgcggg gaatactttt
     2341 cccacaacaa atacgggacc aagaccctgc tcgaattcgc ctcccagaaa gggataaggc
     2401 gcctcatcta cctgagcacg gtttccgttt tcggacatcc cataggggcc gctgatgagg
     2461 atgcgcctta cgcgccggac catccctacg gcgaatccaa actggcaggc gaactgctct
     2521 gcgttgactg gataaagaaa gatccgctcc gggaactcat tgtgttgcgt cccaccgtga
     2581 tcttcggccc ccacaactac gccaacatgt acaaacttct ggataccatg caccgccgac
     2641 cctgggtgat ggtgggcaaa ggcgaccacg ttaaaagcgt ggtatccctg gatagcgtca
     2701 ccggcatggt catattcgcc ctgggcttgc tccagccagg ctttcagcac ttcaactgcg
     2761 ttgacgagcc ttacttaacc ctgcatgaat tgatgcgttt aatcgcctcg cagccaggct
     2821 tcaggatgcc ccgggtgaat attcccatcc aaatggctgt gggtattggc agaatctttg
     2881 acctccctgc caaactgctg gggatcgatt ttcccataaa cagtgaccgc atgaagaaat
     2941 tcgccaccga gaccaatttc caagccgcca agatacgctc ggctggatat gttcagacca
     3001 cgagcatcgc ttccagcatc gccgaaatgt gcgcctggta tctcaagaat cactctcaag
     3061 gagacaagaa ttgaaaaaga taaaattagg attgatcggt tgcggccgca tctccaaaaa
     3121 ccattttgaa gcagtttccc aaattcccga agccgagttt gtcgcctgcg cggatgttgt
     3181 cgaagaaaaa gcccgcctcg ccgccgagac ctatggcata acgaactggt acacagacca
     3241 tctggaaatg ctggacaagg aaaagctcga cgcagtaagc atctgcacac ccagtggact
     3301 ccatccccag ataggcatcg acgtggccaa acacaaagtc aacgtgatca cagaaaagcc
     3361 catggccatc aacatcgacg gcgccagcag gctgatcaag gcctgcgacg ccaaccaagt
     3421 caagctcttc gtggtgaagc aaaaccgcct caacagcacc atgcagctgc tcaaacaggc
     3481 tgtggacaaa aaccgcttcg ggcgcatctt cctggcccag tccaacgtct tctggcaacg
     3541 cccccaagag tattacgacg ctgcaaaatg gcgcgggacc tgggaattcg acggcggcgc
     3601 cttcatgaac caggccagcc attacgtgga tgccatatac tggctgctgg ggaatgtgga
     3661 cagcgtgatg gcctatacct ccaccatggc ccgccggatc gaagctgagg acaccggctg
     3721 cgccatcctc catttccgct ccgggatcat cgccacccta aatgtaacca tgctcaccta
     3781 tccaaagaat ttcgaaggct caatcaccat catcggcgaa aagggcaccg tgaaagtggg
     3841 cggggtggcc gtcaacaaga tagaaaagtg ggaatttgaa gactacgacg acgatgaccg
     3901 catcgccctg gatgccaact atcagccccc gaacgtttac ggattcgggc acaaccccta
     3961 ttaccgcaac gtggtggacg tccttctgaa caagggaaaa cccttcaccg acggaaggga
     4021 cggtcggaaa tccgtggaaa tcatccaggc catctaccag tcagccaaaa caggaacaag
     4081 agtctccctg cccctctgat cccattctaa aacaaagcct ctgacagcgt ttctggtccc
     4141 aaatccggat cagatcctca cgcttttgat cgtgccctgg ttaagtttca gggctggttc
     4201 tccatggatc atcatcacga tgcccagttc ccggccgcaa gcgggacaaa cgggcctcga
     4261 gaaggtcgcc gcccccgtta ggccaacccg gtcctcactc agcctgctga tgaggcattt
     4321 gataagccgc ccctttcccg ccttgaaata gcggaaaagc ctgaagccgc actggcagtc
     4381 gatgtcgatt attttcccgg ttcgtatcat gttttccttg cctgacccgg tattttccct
     4441 gcctgtggag gtcaagggaa atcatggttt cccagtccaa atccttcctt ttccccattt
     4501 attgacggct ctctgtcaaa ccggataggt aaagccctcc aggttgtttt gctgttgagt
     4561 gccagcatcc gctctaatca aaatacagcc ataaatgctc acccaccgcc atgtcctgca
     4621 tgtcaggctc tgagccctcg cttccatccc tctttcatgc cacccgcacg atgcccgcat
     4681 ttggtgcgag gatttagcgg gaggcgcgtc tggggcatag agaagggcct tagcggcaag
     4741 caaccaacgc tatgatcctc tacgggtcac gggcgctgta agaagagggt atcagactgt
     4801 atagtagata gatatggcag tctattaaag ttaattcatt cttttgtgca agctatcact
     4861 atgataagaa acgaattgcc atcttgaata ttacgcgaga aagagctttc ctgactggcg
     4921 tctgttattc gcgggcagac ccaagaccag tcgtttttca gctccgaaaa tcgagtgggg
     4981 tatttctgaa attgggagct ttgatgccgt tttccaataa agttcttgac aatattggcc
     5041 gtttcagagt aaggaaaagt aaaatggcgt gaaaatgaga gaaacagcca tcagggagta
     5101 taacttgctg ttacagaagt attttgagac ccgtttggat atgatcgaag ccagtctgaa
     5161 acaatacatt agtcctcaga cagcaccggc caaggaactc aagaaagcca cgcgctacgc
     5221 tatcttcccc ggcggaaaac gctggcgtcc gcttttgctg cttgcaatgt acgatatgct
     5281 caccgggatg aagggcaaaa gcatggcaga ggccctgaag gccgccacag cgctggaaat
     5341 tgcccacaac gcggccttga cgcacgatga ccttcccttt ttgatgaaca ggacggagcg
     5401 gcgagctgcc ccggcgctgc acatgaaata tgacaacaca gtgggaatcc ttgccggtga
     5461 tgcgctttac gctctcgcct ttgagattat gagcgatatc accccaccct ccaaggcttt
     5521 ggcctgcata cgcatacttt ccatcagcac aaggacatac ggcttgattg gcggccaggc
     5581 ggtggctttg gaaagcaaac accgggtgat gaagctgaac acgttgcgct acat
//
LOCUS       DAOOWR010000084         5598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14622_length_5598_cov_4.526069, whole genome shotgun sequence.
ACCESSION   DAOOWR010000084 DAOOWR010000000
VERSION     DAOOWR010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14622_length_5598_cov_4.526069"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..653)
                     /gene="purE"
                     /locus_tag="PLB85_03115"
     CDS             complement(201..653)
                     /gene="purE"
                     /locus_tag="PLB85_03115"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU72348.1"
                     /translation="MPRVRVILGSRSDLAACEPVKQVLADFGVDYDFHVSSAHRKPEE
                     TARLAKNAEAEGVQVIIAAAGMAAHLPGVIASHTVLPVIGIPLASGGLGGLDALLSIA
                     QMPPGVPVAGVAIGGAKNAALLAVQILALQDPELRAKFRAYKEKLAEG"
     gene            complement(655..1329)
                     /locus_tag="PLB85_03120"
     CDS             complement(655..1329)
                     /locus_tag="PLB85_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOU72349.1"
                     /translation="MAKKFLSKADVAKKLKVSDRVVQEMINSKTFETKLVGKNIKIDE
                     DSLNEWLENLNESEEKMLALKRVICHFEEYMRPENIFLDFEAENKFDAIRILSVKAKE
                     LKLVRDARWLYEVVVAREELISTAIGHGVALLHPRHLHPSKIKTPSILFGRSNIPVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISKLIMNPEILAAFSTASSVEEIHNCLTVL
                     PEQQNK"
     gene            1531..1935
                     /gene="lptE"
                     /locus_tag="PLB85_03125"
     CDS             1531..1935
                     /gene="lptE"
                     /locus_tag="PLB85_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOU72350.1"
                     /translation="MRPFENQSSQFDLGDTVLNGLTQQFSRDGRLKLVTQQPDCTLEG
                     TILDFSESVYSYDSGNNVQDYMLRLSCSVVFTDLVNNQVIYENKNLTLNEAYAADGAD
                     SSTAKSKSKEEATNELVERLFSTIIQNSLETW"
     gene            1877..2659
                     /locus_tag="PLB85_03130"
     CDS             1877..2659
                     /locus_tag="PLB85_03130"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU72351.1"
                     /translation="MSSSNGSSARSSKTASKPGDGTRLNRWLAECGICSRREADELIR
                     SGEVSVNGEPCLDLSRRVGPRDKVSLRGKELRIAEKVYLMLNKPRGYVVTHSDELKRE
                     TIYSLLPESAANLRYAGRLDKNSEGLLLMTNDGEMINALTHPKRRVEKVYRVEVNRRL
                     SRKELEELRRGVQIEGGITLPAGVFVKNDSDKGMTLKMVITEGRKRQIRQMVEAVGAK
                     VLRLKRLQFGTLMLKDLPLGRWRYLSGAELRSLKSLVENPKP"
     gene            2656..3732
                     /locus_tag="PLB85_03135"
     CDS             2656..3732
                     /locus_tag="PLB85_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOU72352.1"
                     /translation="MKIALIGPPKSGKTTIFNALTGSSTSTDKYAPAATEANVGVVQV
                     PDERVTKLSELYHPKKTIYAHIEYRDYPGIFSTQTENPDNALFSDIKSNEGFVLVLRA
                     FQDEELDELFAAGDPARQLASFEDEMLLADLIVAERRIEKIELGYKRGVKTPAIQFEE
                     KILRQVCEHLQSGKPLRELQLQADEEKALRGFRFFSQKPLLVLINCAEDDFHALDGLK
                     QEIAAKGYTTEVIAGRFEEELSRLDVEEAQLFRDDMGISESIRDRFTRLCYSMLGYIS
                     FFTVGEDEVRAWTIEDGDNAVTAAGKIHSDLARGFIRAECFRYTDLMEHGSEKHLREK
                     GLFRLEGKEYIVRDGDIISVRFSV"
     gene            3744..>5598
                     /locus_tag="PLB85_03140"
     CDS             3744..>5598
                     /locus_tag="PLB85_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU72353.1"
                     /translation="MAATRKKSSSKTKRSFGLKLWQKRLIAGFLTLLALLVMISLLFG
                     WESILNDKDHLSLRGNLFRWPFLRGAQVDNPIGVFGVAVGYGISYLFGYHFSLIAAIL
                     TGIISFLYFLEPLAQRKRQKFYLLLIAAFLLQVWLARNIVQPELAMIPKAFWIGLAAV
                     FNTFGATLLLVLGSVFALILFLDYRRLKDFFARLWGELTQGREAAPKKEKAAKPTINK
                     DIPVQPVPQPVIQNHSQDGKQEFPDKPLEDTTPDLKPITGERKKPPASSPETLPDEER
                     EYVMPSIADFLESPVKLSDHDRKEIENQILGTSQVLKDKLAEFGIEAEVRNVNIGPII
                     TQYELEPAKGVKVSKFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNLTRDMIYL
                     RDLLLSEEIRSMDSNLAFGLGKDIAGRPVVTDLAKMPHLLIAGATGSGKSVCINAIIM
                     SFILRSKPDELRLILIDPKRVELAGYNNLPHLIGNVVTEPEQALENMYWAVREMEHRY
                     ELLQEARVRDIGSYNEKAERDPDLEKLPYIVIIVDEFADLILTSGKDIEVPITRLAQM
                     ARAVGIHLILATQRPSIKVITGIIKANFSARIAFQVSSRVDSRVILDQIGAE"
BASE COUNT         1291 a         1568 c         1615 g         1124 t
ORIGIN      
        1 ctgattttag acgccattat ccgccaggtg gacaagcaat atggattacg tgaagcggag
       61 ggctgggact aagccgggcg ttcagacaaa ctgacgacca ggcccggaga ggcaacataa
      121 ccatatcgag ggcacatagt tagtgccccg agggaacccc tcttcgtgag gtagatctaa
      181 gctacactct ggtcagagta tcagccttcg gcgagttttt ccttataggc gcggaatttg
      241 gcccggagtt cagggtcctg cagcgccagt atctgcacag ccagcaaggc ggcgtttttg
      301 gccccgccga tggcaacgcc ggccacaggc acaccagggg gcatttgcgc gatggaaagc
      361 agcgcgtcga ggccgccgag gcctccgctg gcgaggggaa tcccgatcac gggcaaaacg
      421 gtgtgggagg caatgacacc gggcaggtgg gctgccattc cggcggcggc aatgatcacc
      481 tgaacccctt ccgcttcggc gtttttggcc agccgggcgg tttcctccgg ttttctgtgg
      541 gcggaggaaa cgtggaaatc atagtccacc ccaaagtcag ccaaaacctg cttcacaggc
      601 tcgcaggcgg ccaaatcgct ccggctgccg agaatcacgc ggacgcgggg catcttattt
      661 gttttgctgt tcaggcagca ccgtgaggca gttgtggatt tcctccacgc tggaggctgt
      721 gctgaaggcg gccaggatct cggggttcat gatcagcttg gagatgtagg agaggctgaa
      781 gaggtgctgt ttgtcattgt ggagcaggag catgaagaag atgttcacgg gtttgttgtc
      841 cggagcgtcg aaatccaccg ggatgttgga acggccaaag aggatgctgg gggttttgat
      901 cttggaggga tgcagatggc ggggatggag cagagccacg ccgtggccga tggcagtgga
      961 gatgagttcc tcgcgggcca ccaccacctc atagagccag cgggcgtcgc gcaccagctt
     1021 gagttccttg gccttgacgc tgaggatgcg gatggcatca aacttgtttt cggcctcgaa
     1081 atccaggaag atgttttccg gccgcatgta ttcctcaaag tggcagataa cgcgcttgag
     1141 ggcgagcatc ttttcttcgg attcattgag gttttccagc cattcgttga gggaatcttc
     1201 gtcgatcttg atgttttttc ccaccaactt ggtctcaaag gtcttgctgt taatcatttc
     1261 ctgtacaacg cgatcggaga ctttcagctt tttggccacg tccgctttgg acagaaactt
     1321 tttcgccatg gtttacctcc cttggtagtg gcattcataa aaaacctgat cgggtattgt
     1381 gaaaaggttt aacttgacaa agatagagat tattttgagt atggcataat ggtcaagaga
     1441 tttatttggt tcggactgct gtcagcggta cttttgggat gttcatattc cgtatattca
     1501 aatgcttacc cgcatttgaa aaaaattgcc gtgcgcccgt ttgagaacca gagttcccag
     1561 ttcgacctgg gcgatactgt gctcaacggc ctcacgcagc agttcagccg cgacggccgc
     1621 ctgaagctgg tcacccagca gccggactgc acgctggaag ggaccattct ggacttttcc
     1681 gagagtgttt acagctatga cagcggcaac aatgttcagg actatatgct acggctgtcc
     1741 tgcagcgtgg ttttcaccga tctggtgaac aaccaggtga tctacgaaaa caagaatctg
     1801 accctgaacg aggcttacgc ggcggacgga gccgattcca gcaccgccaa atcaaaaagc
     1861 aaagaggaag cgaccaatga gctcgtcgaa cggctcttca gcacgatcat ccaaaacagc
     1921 ctcgaaacct ggtgacggca cacggctgaa ccgctggttg gctgaatgcg ggatttgctc
     1981 gcgccgcgag gcggacgagc tgatccgctc cggcgaggtg agcgttaacg gagagccttg
     2041 tctggacctc agccgcaggg ttggaccccg ggacaaagtg agcctgcggg gcaaggaact
     2101 gcggatcgct gaaaaggtct atttgatgct gaacaagccg cggggctatg tggtgaccca
     2161 ttccgatgaa ttgaagcggg aaaccatcta cagcctgctg ccggaaagtg ccgcaaacct
     2221 gcgctacgcc gggcgcctgg acaaaaactc ggaaggtctg ctgctgatga ccaacgacgg
     2281 cgagatgatc aacgccctca cccatcccaa acgcagggtg gaaaaggttt accgggtgga
     2341 ggtcaaccgc cgcctcagcc gcaaagagct ggaggaactg cgccgcgggg tgcagatcga
     2401 aggcggaatc acgcttccgg caggagtgtt cgttaaaaac gacagcgata agggcatgac
     2461 cctgaagatg gtgataaccg aaggccgcaa acgccagatc cgccagatgg tggaggctgt
     2521 gggggccaag gtgctgcgcc tgaaacgcct gcaattcggc accctgatgc tcaaagacct
     2581 gccgctgggc agatggcgct atctgagcgg agcggaactg cgctccctga aaagccttgt
     2641 ggagaatccc aaaccatgaa aatagccctc atcggaccgc ccaaaagcgg caagaccacc
     2701 attttcaatg ccctcaccgg aagctctacc agcacagata aatacgcccc cgcggccacc
     2761 gaagccaacg ttggcgtggt gcaggttccg gatgaacggg tgaccaaact cagcgaactt
     2821 taccacccca aaaagaccat ttacgcccat atcgaatacc gcgactatcc cggcatcttc
     2881 tccacccaaa cagagaatcc ggacaacgct ctcttttccg acatcaagtc caacgagggt
     2941 ttcgtgctgg tgctacgcgc ctttcaggac gaggagctgg acgaactgtt cgccgctggc
     3001 gatcccgcgc ggcaactggc ctcgtttgaa gacgagatgc tgctggcgga cctgatcgtg
     3061 gcggagcggc ggatcgaaaa gattgaactg ggctacaaaa ggggtgtgaa aacccccgcc
     3121 atccagttcg aggaaaagat tttgcgccag gtttgcgaac acctgcagag cggaaagccc
     3181 ctgcgcgaac tgcaactgca agcggatgag gaaaaggccc tgcgcggctt ccgcttcttc
     3241 agccagaaac cgctgctggt gctgatcaac tgcgccgagg acgactttca cgccctggac
     3301 gggctcaagc aggaaatcgc cgccaaaggc tacaccacag aagtgatcgc gggccgcttt
     3361 gaagaagaat tgagcaggct ggacgtcgag gaagcgcagc ttttccgcga tgacatgggc
     3421 atcagcgaaa gcatccgcga ccgcttcacc cggctctgct acagcatgct gggctatatc
     3481 agctttttca ccgtgggcga ggacgaggtg cgcgcctgga ccatcgagga cggcgacaat
     3541 gccgtcacgg ccgccgggaa aatccattca gacctggccc ggggcttcat ccgggcggaa
     3601 tgtttccgct atactgacct gatggagcac ggtagcgaaa agcatctgcg cgaaaaaggc
     3661 ctcttccggc tggaaggcaa ggaatacatc gtgcgcgacg gcgacatcat ctccgtgcgc
     3721 ttcagcgttt aggagcgaac caaatggctg ccaccagaaa gaaaagcagt tccaagacta
     3781 aacgttcctt cggcctgaag ctctggcaga aacgcctcat tgcaggcttt ttgaccctgc
     3841 tggcactgct ggtgatgatc agtctgctct ttggctggga atccatcctt aacgacaaag
     3901 accacctgag cctgcgcgga aaccttttcc gctggccctt cctgagagga gcgcaggtgg
     3961 acaatcccat cggagtgttc ggtgtggcgg tgggttatgg catttcctac ctctttggct
     4021 atcatttttc gctgatcgcg gccatcctca cgggcatcat cagctttctc tatttccttg
     4081 aacccctggc acagcgcaag cggcagaagt tttatctgct gctgatcgcg gcttttctgc
     4141 tacaggtgtg gctggccagg aacatcgtgc agccggaact tgccatgatc cccaaggctt
     4201 tctggatcgg cctggcggcg gtgttcaata ccttcggggc cactttgctg ctggttttgg
     4261 gcagcgtatt tgccctgatc ctcttcctgg attaccggcg cctcaaggat ttctttgcca
     4321 ggctctgggg ggaactgacc cagggcaggg aagccgctcc caaaaaggaa aaagcggcca
     4381 agccgaccat caacaaggac attcccgtcc agccggtgcc tcaacctgtg atccaaaacc
     4441 attcccaaga cgggaaacag gaatttccgg acaagcccct tgaagatact accccggacc
     4501 tcaaacccat caccggagag cggaaaaagc cgcctgcatc cagtccggaa accctgccgg
     4561 atgaggaacg cgagtatgtg atgccctcga tcgcggattt tttggaaagc cccgtcaagc
     4621 tttccgacca cgaccgcaaa gagatagaaa accagatttt gggcaccagc caggtgttga
     4681 aggacaagct ggcggaattc ggcattgagg cggaagtgcg caacgtgaat atcgggccga
     4741 tcatcacgca gtatgagctg gaacccgcca agggcgtgaa ggtgagcaaa ttcacctccc
     4801 tggccgacga cctggccctg gccatcaaag ccaaatccat ccgcgtgcag gctcccatcc
     4861 cgggacgcgg cctgatcggc atcgaaatac ccaacctcac acgcgacatg atctatctgc
     4921 gcgacctgct gctttccgaa gagatcaggt ctatggattc caacctggct ttcggcctgg
     4981 gcaaggacat tgcaggacgg cctgtggtca ctgacctggc caaaatgccg cacctgctga
     5041 tagccggcgc caccggcagc ggcaaaagcg tctgcatcaa cgccatcatc atgagcttca
     5101 ttttgcgctc caaaccggat gaactgcgcc tgatcctgat cgatcccaaa cgggtggaac
     5161 tggccggcta caacaacctg ccccacctga tcggcaatgt ggtcacggaa ccggaacagg
     5221 ctttggaaaa catgtattgg gccgtgcgcg agatggaaca ccgctacgaa ctgcttcagg
     5281 aagcccgggt gcgcgatatc ggctcctaca acgaaaaggc ggagcgggac cctgatttgg
     5341 aaaagctgcc ctacatcgtg ataatcgtgg atgaattcgc cgaccttatc ctcaccagcg
     5401 gcaaggacat tgaggtgccc atcaccagac tggcccagat ggcccgcgcc gtgggcatcc
     5461 atctaatttt ggccacccag aggccttcta taaaggtcat caccggcatc atcaaagcga
     5521 acttctccgc ccgcatagcc ttccaggtct cgtcccgggt ggattcccgc gtgattttgg
     5581 accagatcgg agccgaac
//
LOCUS       DAOOWR010000085         5576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14698_length_5576_cov_3.878645, whole genome shotgun sequence.
ACCESSION   DAOOWR010000085 DAOOWR010000000
VERSION     DAOOWR010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14698_length_5576_cov_3.878645"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="recF"
                     /locus_tag="PLB85_03145"
     CDS             complement(<1..936)
                     /gene="recF"
                     /locus_tag="PLB85_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOU72354.1"
                     /translation="MILKQLSLANFRNYEQASFSLRPEGNLVIAPNGWGKTNLLESIA
                     YCGLGKSVRFHRDHELKRENTDFFSVRGEFEDDSGLDLKVQISWQQGRKLLKINDIPV
                     KQLSRIFENVKVIYAAPEDMNLVGGYPRFRRQYFDLAISQIYPPYITTLREYLHVVEQ
                     RNSLFKRPYTREEKLAWDTKFCSLLLEVLDYRKRYLELVNGAFAQRYPEVSEQVKDIR
                     VLYLPHLNEDYPATAGQMLETLAALESREKRYQRSLAGAHIDDYEFRLNGHNLKTYGS
                     QGQKRITVIILKLIQAALIQELTGIRPILLFDDVFA"
     gene            complement(940..2106)
                     /gene="dnaN"
                     /locus_tag="PLB85_03150"
     CDS             complement(940..2106)
                     /gene="dnaN"
                     /locus_tag="PLB85_03150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU72355.1"
                     /translation="MRLAIEQKELVHHIQHLVGIISAKTTSPILTNYLIEANADKGEV
                     KITASDLELTVDVRFNAAVSEGGTIAVSAKHFNEIIQQMPPAVIDLWKTEDLLMIQCT
                     KIDFNILCADHTLFPILPEPKLENATIVNAETFNRMISKTSFAVSTDVNRAVLTGVCW
                     KIMEKTNLMAATDGRKVAEIIVPNTAPEKQEKAEGEESVFQYAPSEQLLEKVIPVKTL
                     NFLQKVFDSSIKEMKVLMERNKIIFSYGAFTIVSNILEHKYPEYQKAFMADLPNQIVV
                     EKKKLQESIRRVALVAPEDNNRIRFEIDSERFEINTSDRDTGDAKEFVEDFKFTGSET
                     SISFNFRYMLSILDAIDTDTVCIKLGGSREPMMVYNEPLHENQKITFLLMPLRS"
     gene            complement(2228..4786)
                     /locus_tag="PLB85_03155"
     CDS             complement(2228..4786)
                     /locus_tag="PLB85_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOU72356.1"
                     /translation="MNRLTRLAATAAILTFLALTCGLQGAYIDKMEIKATQPDGTTLE
                     LFASGDEFHNWLHDAEGFTIIRHPQTGWLCYAVKDGDDVAAGPLIVGNGDPRAYGLEP
                     GINISEELYKQRRKERFPMPAERDAPTTGTINNLVIYIRFSDETEFGQQNSVYDGWFN
                     SSTNSQKNYFLEASYNQLTVNTHFYPTPSGGYVVSWQDSHPRAYYQPYNAATNPTGYS
                     GDYQRRSREFTLLQNATNGVRSQIPSSLNIDSDGDGRVDNVVYIVKGAAGEWSSLLWP
                     HRWSLYDRYVYINGKRVYDFNFQLQDFLANRSVGVICHEFFHTLGAPDLYHYVSNGIN
                     PAGNWDLMDTNFNPPQHMTAFMKYKYGDWIDSIPTLSEDGVYTLNPLTSATGQCFRIN
                     SNNPSQYYVVEFRKKTGTFESSLPGSGLIVYRIDNSVEYGNADGPPDELYIYRPGGTT
                     TVNGNVNTANFSYETGRTAINSTTNPTPFLQDGSAGNLYICEIGSSAGNTISFRLGPP
                     VFDFSVNPHLQGFDATDFPPLGWAKSAEAGNYQFERVTSGTNPSCSPQSGAGMLRYNS
                     DIAPVGYSALLITPRIVVSDAVNHAHAVSFWMYRDANQSTALDKIEIYLNTSANMNGA
                     PSLLGTIHRARQQAPTEATPGWKNYIYPLPINTAGSYYVIFRAVSAAGYNMFLDNIKV
                     AQVPFPTNCLSPTDQANHVATDCQFSWQPALGSPTGYKLYLGTDNPPTNLLNGQDLGV
                     ASSYEYSSLLAPETTYYWKVVPYNAGGEALDCSIWSFGTLSTAPPLVAISEGSGGLHL
                     AWSALPGYAGYHVFASDDPTNWPDTPSATVYEPQYSIPPGTERHKFYKVQAFTD"
     gene            complement(5047..>5576)
                     /locus_tag="PLB85_03160"
     CDS             complement(5047..>5576)
                     /locus_tag="PLB85_03160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72357.1"
                     /translation="SNISTSKGSADLLQALKLLSLKTKDWHLSWGGAGPLLNWARQKA
                     EDEGLRDHISWLGRLSSTQSLEQYQLCDFYVMPSRFESFSLVIAEALACGKPVVATNC
                     GGPRDLVNDLCGILVPKQNPAALAEALLKMGANFSSYSPQAIREYCVQNFGHKAVTAR
                     IGELYQSVFNAKGTP"
BASE COUNT         1152 a         1379 c         1633 g         1412 t
ORIGIN      
        1 ggcgaaaaca tcgtcgaaga gcaggatggg cctgatgcct gtgagttcct ggatcagggc
       61 ggcctggata agtttgagga tgattacggt gatccgtttt tggccttggg agccgtaagt
      121 tttcaagttg tgtccgttca ggcggaattc gtaatcgtcg atgtgggccc cggccaggct
      181 tcgctggtag cgtttttccc gggattccaa ggctgccagg gtttcgagca tttgcccggc
      241 tgtggcagga tagtcttcat taagatgggg cagataaagc acgcggatgt ccttcacttg
      301 ctcagaaacc tctggataac gctgcgcgaa agccccgttc acgagttcca gatagcgctt
      361 gcggtaatca agcacctcca gcagcaggga gcaaaacttc gtgtcccagg ctagcttttc
      421 ctcgcgggtg tagggacgtt tgaagaggct gttgcgctgc tccaccacgt gcaagtattc
      481 gcgcaaagtg gtgatgtagg gcggatagat ctgggatatg gccagatcga agtactggcg
      541 ccgaaagcgg ggatatccgc ccaccaggtt catatcttcc ggagcggcgt agatcacctt
      601 tacgttttcg aagatccggc ttagctgctt tacaggaatg tcattgattt tcagcagctt
      661 tctgccctgc tgccaactta tctgcacctt caggtcgagg cctgagtcat cctcgaattc
      721 gccccgaacg ctgaagaaat ctgtgttttc ccgcttcagt tcgtgatccc ggtgaaagcg
      781 cacggatttg cccaaaccgc aataggcaat ggattcaagc agattggttt tgccccagcc
      841 gttgggtgcg atcaccagat tcccttccgg ccggaggctg aaactggctt gttcgtaatt
      901 gcggaaattg gccaggctaa gttgtttcag gatcaatcgc tacgaccgca gcggcatcaa
      961 gaggaaagtg atcttctggt tttcgtgcag cggttcgttg taaaccatca tcggttctct
     1021 agagccgccc agcttgatgc agaccgtgtc cgtgtcgatg gcatccagaa tcgacagcat
     1081 atagcggaaa ttgaaggaga tggaggtctc ggagccggtg aatttgaagt cttccacaaa
     1141 ttccttggcg tcgccggtat cgcggtcgct ggtgttgatt tcgaagcgtt cggaatcgat
     1201 ctcaaagcga atgcggttgt tgtcttccgg cgccaccaga gccacgcggc ggatggattc
     1261 ctgcagtttc ttcttttcca ccacaatctg gtttggcaaa tcggccatga aggctttctg
     1321 atactcgggg tatttatgct ccaggatatt ggaaacgatg gtgaaagcgc cataggagaa
     1381 gattattttg ttgcgttcca tcagcacctt catttccttg atggaagagt cgaaaacctt
     1441 ttgcaggaag ttcagggttt tcaccggaat caccttctcc agcagctgtt cagaaggcgc
     1501 gtattggaag acagattctt cgccctcggc tttttcctgc ttctccgggg cagtgttcgg
     1561 gacaatgatc tccgcgactt tgcgtccgtc cgtggcggcc atcaagttcg tcttttccat
     1621 aatcttccag caaacacctg tgagcaccgc gcggttcaca tcggtggaaa cggcgaagga
     1681 ggttttgctg atcatgcgat tgaaggtctc ggcattcacg atggtggcgt tttcaagctt
     1741 gggttccggc aggatcggaa acagagtgtg gtcggcacag aggatgttga aatcgatctt
     1801 ggtgcactgg atcatcagca ggtcttcggt tttccagagg tcgataaccg caggtggcat
     1861 ctgctggatg atttcgttga agtgcttggc agaaacggcg atggtgccgc cttcgctaac
     1921 agccgcgtta aaacgcacgt ccacggttag ttccagatcg gaggcagtga ttttcacctc
     1981 gcctttgtcg gcattggctt cgatcaggta gttggtcaaa atgggagagg tggtcttggc
     2041 ggaaatgatt cccaccaagt gttggatgtg gtggacaagt tctttctgct ctatcgctaa
     2101 tctcatgtct tccccttgtt tacagtaatt ctatttaaaa accaccaaat ttggcaattg
     2161 gctgtttgtc aactgatttg tgaacttggc attttttccg gtatcggaga aatggctccc
     2221 gcggccatta atctgtgaag gcttgcacct tgtagaattt gtggcgctcc gtgcccggag
     2281 gaatgctgta ctggggctca tagacagttg cagaaggtgt gtccggccag ttggtgggat
     2341 cgtcgctggc aaagacatga tagccggcat atcccggaag cgctgaccaa gcaaggtgta
     2401 ggcctcctga accttcggag atggccacca gcggcggagc ggtgctcagg gtcccgaaac
     2461 tccagatgga acagtccaag gcttcaccgc cggcgttgta aggaacaact ttccagtaat
     2521 atgtggtttc cggcgcgagc agggagctat attcgtaact ggaggcaaca cccaggtcct
     2581 gaccattcag cagattcgtg ggagggttgt cggttcccag atagagctta tatcctgtgg
     2641 gggagcccaa agcgggctgc cagctgaact ggcagtcggt ggcaacgtgg ttggcctggt
     2701 ctgtagggct caggcagtta gttgggaaag gtacctgcgc cactttgata ttgtccagaa
     2761 acatattgta gcccgcagcg ctcacagccc ggaagataac gtagtaactg cccgcagtat
     2821 ttataggcag ggggtaaata taatttttcc atccgggtgt ggcttcggtt ggagcttgct
     2881 gcctggcacg atgaattgtt cccagcaggc tgggcgcacc gttcatgttc gctgaagtat
     2941 tcagatatat ttcgatcttg tccagcgccg tgctttggtt cgcgtcccgg tacatccaga
     3001 aactcaccgc gtgggcgtgg ttgacagcgt cgctgaccac gattctgggc gtgatgagca
     3061 gggcggagta accaaccggg gctatatcgc tgttgtagcg cagcataccg gctccggatt
     3121 gggggctgca ggatggattt gttccagagg taacgcgttc aaactgatag tttcccgcct
     3181 cggcggattt ggcccatccc agagggggaa agtcggtggc gtcaaaaccc tgcagatgag
     3241 ggttcacgct gaaatcgaac actggcgggc ccaagcggaa acttatggta ttaccagcgc
     3301 tggagccaat ttcgcagatg tagaggttcc cggctgaacc atcttgcaaa aagggagtcg
     3361 gattggttgt tgagttgatc gccgtgcgcc cggtttcata gctgaagttt gcagtgttaa
     3421 cgtttccatt tacagtggtc gtcccgcccg gccggtagat gtagagttcg tcaggtggcc
     3481 cgtccgcgtt accgtattct actgagttgt cgatcctgta aacgatcagt cccgaacccg
     3541 ggagggagct ttcaaaggtt ccagtcttct tgcggaactc caccacatag tactggctgg
     3601 ggttattgga gttgatccgg aaacactgtc cggtggccga ggtcagcgga tttagagtgt
     3661 aaaccccatc ctcgctgagg gtgggaatgc tatctatcca atcgccgtat ttgtatttca
     3721 taaaagcggt catgtgctgg ggaggattaa agttcgtatc catcaggtcc caattcccgg
     3781 cggggttgat gccgttgctt acgtaatgat acaggtcggg cgcgcccagg gtgtggaaaa
     3841 actcgtggca gataacccct acgctgcggt tggccagaaa atcctggagc tggaagttga
     3901 agtcatagac ccgcttgccg ttgatgtaca catagcggtc atagagggac caacgatgcg
     3961 gccagagcag gctggaccat tctcccgcgg cgcctttcac gatatagacc acgttgtcaa
     4021 cccggccatc accgtcggaa tcgatgttca ggctgcttgg tatctgggag cgcacgccgt
     4081 tcgtagcgtt ttggagcagt gtgaattctc tgctcctgcg ctgataatct ccgctatagc
     4141 ctgtgggatt ggtggcggcg ttgtagggct ggtaataagc ccggggatgg ctgtcctgcc
     4201 aggagaccac atatcctccg gagggggtgg gataaaagtg tgtgttcaca gtcagttggt
     4261 tgtagctggc ttccaggaag tagtttttct gggagttggt ggaagagttg aaccaaccgt
     4321 cataaacgct gttttgctgg ccgaattccg tctcatcgga gaagcggatg tagatcacca
     4381 ggttgttgat ggttccggtg gtgggcgcgt cccgctctgc tggcatgggg aaacgctctt
     4441 tgcgcctctg tttgtaaagc tcttcgctga tgttgatgcc aggctcaagt ccgtatgcgc
     4501 gggggtcgcc gttacccact attaggggcc cggcagccac gtcgtccccg tccttcacag
     4561 cgtagcagag ccagccggtc tggggatggc ggatgatggt gaagccttcg gcatcgtgaa
     4621 gccagttgtg gaattcgtca ccgctggcaa agagttccag cgtcgttccg tcgggttggg
     4681 tagccttaat ttccatcttg tcgatgtagg ccccctgcaa tccgcaggtc agggccagaa
     4741 acgtcagaat ggcggctgtg gcggcgagtc gggtaagtct gttcacttct cattctccca
     4801 ctaggttcat ttttctcagt ttatccggct ggcgatatcc gtcaatgttt ttcccgctat
     4861 cccaattttt ctctgcaaaa tgataccatc atgttccgtc ctttgcccct tcccatccgc
     4921 ctctttttcg cctcccgcac agttcccgtt cagcaggcgg gaactcagcg ggtagtgaat
     4981 gggatgtgaa tgggaagggg caaaggacga gtcaaccggc gaggcaaggg attgtccgga
     5041 aacgggttat ggcgtgcctt tggcgttaaa aacagattga taaagctcac caatccgggc
     5101 ggtaacagct ttatggccaa agttttggac acagtattcc cggatggctt gaggagaata
     5161 gcttgaaaag ttggctccca ttttcagcag agcttccgcg agggcggcag gattctgttt
     5221 ggggaccagg atgccgcaga ggtcgttcac gaggtccctg gggcctccgc agttggttgc
     5281 caccaccggt tttccacaag ccagcgcttc agctatcacc aggctgaagc tttcgaaacg
     5341 gcttggcatc acatagaagt cgcaaagttg atactgctcc agggattgag ttgagctaag
     5401 ccttccaagc cagctgatgt ggtcacgcaa tccctcatcc tcggcttttt gccttgccca
     5461 gttcagcaga gggcctgccc cgccccagga caggtgccag tctttcgttt tcaaacttaa
     5521 aagcttcagc gcctgaagca ggtcggcgga ccctttgctc gtgctgatat tggaca
//
LOCUS       DAOOWR010000086         5541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14803_length_5541_cov_3.389172, whole genome shotgun sequence.
ACCESSION   DAOOWR010000086 DAOOWR010000000
VERSION     DAOOWR010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_5541_cov_3.389172"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /gene="rpe"
                     /locus_tag="PLB85_03165"
     CDS             <1..548
                     /gene="rpe"
                     /locus_tag="PLB85_03165"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU72358.1"
                     /translation="IMDGHYVPNLSFGQPLVSRIRQLSDLPLDAHLMVTNPAAYVDPL
                     ADLGVGWLSFHQECEYHVHRLVQRIKSRGMKAGIALNPAVPLSTLDSILPELDYVLLM
                     SVNPGFSGQNFIPSVLDKTATLSEMIALRGLPTLIEVDGGVNAETAPGLISSGAGILV
                     SASHIFSSHDRAAAIHSLRVS"
     gene            837..2162
                     /gene="ffh"
                     /locus_tag="PLB85_03170"
     CDS             837..2162
                     /gene="ffh"
                     /locus_tag="PLB85_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU72359.1"
                     /translation="MFSNLSEKLDKVFRNIRGKGYLTEQNIKDALREIRLALLEADVN
                     FRIVKNFVAEVEKRALGAEVMKSLTPGQQVIKIVHEQLIALMDTEGFELKAYNNKLTK
                     IMLVGLQGSGKTTACAKLALHFRKKNIKPMMVACDVHRPAAIDQLQILGKQINIPVIA
                     EDSKDVLRIADTALRQAQKDLINLLVFDTAGRLHIDEVMMDEVLRLKSFVKPDYIFFV
                     ADAMTGQEAVNVAKEFYDKLAFDAVILTKLDGDARGGAALSIKAVTERPVAFVGVGEK
                     INDLEVFHPDRMASRILGMGDVLSLIEKAEEAVDAKESEKLAKKMLKNQFTLNDFLKQ
                     LQSIKKMGSLESIIRMIPGLGHKLPADLKIDDNALKHVEAIIQSMTDRERERPDILNG
                     SRRLRIAKGCGRSVMEVNRLLRQFEDMKKMMKKFSTPQGMKDFEKLMSK"
     gene            2184..>5541
                     /locus_tag="PLB85_03175"
     CDS             2184..>5541
                     /locus_tag="PLB85_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72360.1"
                     /translation="MKKILLFLSLSVLLALSLAAETITVGGGINSVTVLSSSGTETLL
                     QYQIGRFEQTRVQIGGEDWYHIDLPKEGFTQEKGTPQLPVFNRSIIIDPTAKMRLELL
                     DVQYQDFPVAVAPSKGVITRNINPDTVPYSFGDVYQRDAFYPQNVAELSEPYIMRDFR
                     GIVVQTTPFAYNPKTKTLRVFTSYKVRVYASGQDTVNTIITSRSSISRDFASLYENRF
                     LNWDSFRYTPVNDSFGKLLVICHTNYMSTIQPYVNWKIQKGIPTELVEWSTIGTTAAQ
                     LQTYIQNRYNADNDLTYVQLVGDAPQIPTLTHNNGGSDPSFSLVAGSDNYPDIFVGRF
                     SAETAAQVTAQINKAIAYERDVTTTDNWLSRALGIASDEGGSGGENGESDIAHMNNIR
                     TDLLNYGYTSVDQIYDPGASASTVTTNVNAGRGFMNYVGHGSDTSWVTTGFNNNNASA
                     LTNGTKTPFIMDVACVNGNFVSMTCFAEAWLRNANGGAVAMYASSINQSWSSPMRAQD
                     EVTDLLIAEAKTTVGGLYYNGSCRMIDVYGSDGVNMFKTWHIFGDASLSSRTRTPIAM
                     NVTHPSSITSGTTSVNVSTGVNNTLVAITYNNVIYGRAFTNSSGNATITLSNPPTGTL
                     NYTITATAHNRVTYVGTIAQTVAAGPWMEVTAANYDDANNDIPEYNESGYFDVTFKNS
                     GTSAASSVTAILSCSTSGITITDNSHTISSLAAGASVQANDAYAITIANNVADGTVAN
                     FTITMTMSGYDPWTYNFSKTINAPALAFGNMTISDPTGDNDGLLDPGETATISIVLNN
                     SGGAASLAGSGTLTCSTSGITVVNGSVSFTAISAGGYATLNFTVTAASSMAEGTLAAF
                     VFNAVAGQYSANTTVNVEVGAPMEVVIGNGTSTQSYPIDRYYNYSSHEAVYLTSEIGT
                     AGTIKSIGFYKASGADVNPIESVTVYMKNTSSNTLADGTYSTTGYTQVYSGTWPNTAT
                     SGWMEVNLDNQFAYDGSNLSILTIKGNQAWTSNYPVWTYTTASPARARQSRNDNYQPT
                     SLTASTNLPNLKLKIFPTPGMLYPPRELTASATHGSVTLTWQAPITGTPTGYKVYKNN
                     TALTTVTGLTYTDTAVTDGTTYSYKVTALYG"
BASE COUNT         1365 a         1710 c         1348 g         1118 t
ORIGIN      
        1 atatcatgga cgggcattat gtgcccaatc tttcttttgg ccagcctctt gtctccagga
       61 taaggcagct ttccgatttg cccctggacg cccatctgat ggtcaccaat cccgccgcct
      121 atgttgaccc cctggccgat ctgggggtag ggtggttgag tttccaccag gaatgcgaat
      181 atcacgttca caggctggtg caacggatca agagccgcgg catgaaggcc ggaatagccc
      241 tcaatccggc agtgccgctc tccactctgg attccatcct gcccgaactg gattacgtcc
      301 tgctaatgag cgttaatccc gggttttccg ggcagaattt catcccctcg gtactggata
      361 aaacagccac tctcagcgaa atgatcgcgc tgcgcggctt gcccaccctt attgaggtcg
      421 atggcggcgt taacgctgaa acggctcccg gcctgatttc cagcggtgcc ggcatcctcg
      481 tttccgcctc ccacattttc agttcccacg atcgcgcggc agccatccac tctcttaggg
      541 tgagttgacc gcgatgccta tgtttttaac tagccgttcc gcaaccattt tgcatccatg
      601 caaatatcgc ggctttcaac ctttcacgtc tgtgccccga agaaccggcc catacgcctc
      661 cttaggcccc aatcatacgt tgcgtgaacg cctcccgcat aatactcgca tttcatgcgg
      721 ggattatccg ggacgcgtgc gggatgcgaa caagctcggg ttaaggacgg attcaggatc
      781 gtccaacggc cttatagtga gttgacagat aatgataata agcatcggga ccctttatgt
      841 ttagcaatct ctcagaaaaa ttggataaag tcttccgcaa catccgcggc aagggctatc
      901 taaccgaaca gaacatcaaa gacgcgctgc gcgagattcg gctggcactt ttggaagcgg
      961 atgtcaattt ccgcattgtg aaaaacttcg tggcggaagt ggaaaagcgc gcgcttggcg
     1021 ccgaggttat gaagtccctc acgcctgggc aacaggttat caagatagtt cacgaacagc
     1081 tcatcgcgct gatggacacc gagggcttcg agctcaaggc ctacaacaac aagctcacca
     1141 aaatcatgct ggtgggtttg cagggctcgg gcaaaaccac cgcctgcgcc aagctggccc
     1201 tgcatttcag gaaaaagaac atcaagccga tgatggttgc ctgtgacgtt caccgtcccg
     1261 ccgccatcga ccagctgcaa atcctgggta agcagattaa catccccgta atcgcggaag
     1321 acagcaaaga cgtgctgcgg atagccgata ccgctctgcg ccaggcccag aaggacctca
     1381 tcaacctgct cgtgtttgac accgcgggac gcctgcacat cgacgaagtg atgatggatg
     1441 aggtgctgcg gctcaaatcc tttgtcaagc cagattacat cttcttcgtg gcggatgcca
     1501 tgaccggaca ggaagcggtg aacgtggcca aggagtttta tgacaaactg gccttcgacg
     1561 ccgttatcct aaccaagctg gacggtgacg cccgcggcgg tgcggctctt tccatcaaag
     1621 cagtcaccga gcgcccggtt gcctttgtgg gcgttggtga aaaaatcaat gaccttgagg
     1681 ttttccatcc ggaccgcatg gcctcgagaa ttttgggcat gggcgatgtg ctcagcctga
     1741 tcgaaaaggc ggaggaagcg gttgacgcca aggaaagcga gaaactggcc aaaaagatgc
     1801 tcaaaaacca gttcaccttg aacgactttc tcaagcaact ccagagcatc aaaaagatgg
     1861 gctccctaga atccatcatc agaatgattc ccggcctggg ccacaagctg ccggccgacc
     1921 tgaaaatcga cgacaacgcc ctcaaacacg tggaggcgat catccagtcc atgacagacc
     1981 gggaacgcga acggcccgac atcctcaatg gcagccgcag attgcgcatc gccaaaggct
     2041 gcggccgcag cgtgatggaa gtgaaccggc tgttgcgcca gtttgaggac atgaagaaga
     2101 tgatgaagaa attttccact cctcagggca tgaaagactt tgaaaaactt atgagcaaat
     2161 aacaaacgtc aggaggaaaa ctgatgaaaa aaatcctgtt attcctgtct ctgagcgttc
     2221 tgctggcgct aagcctggca gcggagacaa tcaccgtcgg aggcggaatc aattccgtca
     2281 ccgtgctttc ttcttcagga accgagaccc tgctgcagta tcagatcggc agatttgaac
     2341 aaacacgggt tcagatcggg ggcgaggatt ggtatcatat cgacctgccc aaggaagggt
     2401 tcacccaaga aaagggtact ccccagctac cggtcttcaa ccgcagcatt atcatcgacc
     2461 ccactgccaa aatgagactt gagctcctgg acgtgcagta tcaggatttc cccgtcgctg
     2521 ttgccccgtc caaaggtgtc atcacccgca acatcaatcc ggataccgtt ccttacagct
     2581 ttggggatgt gtatcagcgg gatgcttttt acccgcaaaa tgtggcggaa ctctccgaac
     2641 cctacatcat gagggatttt cggggcatcg tggtccagac cacacctttc gcctacaacc
     2701 caaaaaccaa aactttgcgt gttttcacat cctacaaggt ccgcgtttat gccagcggac
     2761 aggataccgt gaacaccatc atcaccagca gaagcagcat ttcccgcgat tttgcctcgc
     2821 tctatgaaaa ccgtttcctg aactgggaca gcttccgcta cacccctgtt aatgatagct
     2881 tcggcaagct gctggtgatt tgccacacca actacatgag caccatccag ccctatgtga
     2941 actggaaaat ccaaaaagga atccccacgg aactagtgga atggtccacc atcggcacta
     3001 ccgctgccca actgcagacc tacatccaga atcgctacaa cgcggacaac gacctcacct
     3061 atgtgcagtt ggttggcgat gctccccaga ttccaaccct gacccacaat aacggcggct
     3121 ctgacccgtc cttctccctg gttgccggtt cggataacta ccccgatatt ttcgtcggcc
     3181 gcttctccgc cgaaacagca gcccaggtca cagcccagat caacaaggct atcgcctatg
     3241 aaagagacgt gaccaccacc gacaactggc tgagccgtgc cttgggtatc gcctccgatg
     3301 aaggcggcag cggcggcgaa aacggcgaat cagacatagc gcacatgaac aatatccgca
     3361 ctgacctgct caactacggc tacacatctg ttgatcagat ctacgaccca ggtgcctccg
     3421 cttccaccgt gactaccaat gtcaatgccg gccgtggttt catgaactat gtcggccatg
     3481 ggtcagacac aagctgggtc accaccggat tcaacaacaa caatgcctca gcgctcacca
     3541 atggcacgaa aacgcccttc atcatggatg tggcctgcgt gaacggcaac ttcgtctcca
     3601 tgacctgttt tgccgaagcc tggctacgca atgccaatgg cggtgcagtg gcaatgtacg
     3661 cctcttccat caaccaatcc tggagctcgc cgatgcgcgc ccaggatgag gtgactgacc
     3721 tgctgatcgc cgaagccaaa acaactgtcg gcggcctgta ttacaacggt tcctgcagga
     3781 tgatagatgt ctatggctcc gacggcgtga atatgttcaa aacctggcac atctttggtg
     3841 acgccagcct ctcctcacgc accaggaccc ccatcgccat gaacgtgacc cacccctcca
     3901 gcatcacatc tggaaccact tccgtaaacg tgtccaccgg agtcaacaac accctggtgg
     3961 cgatcactta caacaacgtg atctacggcc gcgctttcac caacagctcc ggtaacgcca
     4021 ccatcacgct tagcaaccca cccactggca cgctgaacta caccatcaca gccactgcgc
     4081 acaaccgcgt tacctatgtg ggcaccattg cccagaccgt ggccgctggt ccctggatgg
     4141 aagtgaccgc cgcgaactat gacgacgcga acaacgacat tccggaatac aacgaaagcg
     4201 gctattttga tgtcaccttc aagaactccg gcaccagcgc cgccagcagc gtaaccgcca
     4261 ttttgagctg ctccaccagt ggcatcacca tcactgacaa cagccatacc atcagtagcc
     4321 tggcagcagg tgcttcagtt caggcaaacg acgcctacgc gatcacgatc gccaataacg
     4381 tcgccgacgg caccgtagcc aacttcacca tcacaatgac catgagtggc tacgatccct
     4441 ggacttacaa cttcagcaag accatcaacg ctcctgctct ggcctttggc aatatgacga
     4501 tcagtgatcc caccggcgat aacgatggcc tgctcgatcc gggggagacc gccacaatct
     4561 ccatcgtact gaataactcc gggggagctg cctccctggc tggctccgga accctgacct
     4621 gctccaccag tggcatcacc gtggtgaacg gcagcgtcag cttcactgct atttccgcag
     4681 gtggctatgc caccttgaac ttcactgtga cagccgcctc ctcaatggcg gagggcactt
     4741 tggcagcctt cgtcttcaac gctgtcgcgg gacagtacag cgccaacacc accgtgaacg
     4801 tggaagtggg cgccccgatg gaagtggtca tcggcaacgg tacctccacc cagagctacc
     4861 ccattgaccg ctactacaac tattccagcc atgaagccgt ctacctgact tccgagatcg
     4921 gaactgccgg taccatcaag tcaatcggtt tttacaaagc cagcggagct gatgtgaatc
     4981 ccattgagtc tgtgaccgtc tatatgaaaa acaccagttc caacaccctg gcggatggaa
     5041 cctattccac cacaggctac acccaggttt acagcggcac ctggcccaac accgccacca
     5101 gcggctggat ggaggttaat ctggacaacc agttcgccta tgacggcagc aacctctcca
     5161 tcctcaccat caagggcaac caggcttgga catccaacta tcccgtgtgg acatatacca
     5221 cagcctcacc cgcccgcgcc aggcagagca gaaacgacaa ttaccagcct acttcgctga
     5281 ctgccagcac caatctgccc aaccttaagt tgaagatctt ccccaccccg ggaatgctct
     5341 atcctccgcg ggaactgacc gccagcgcca cccacggatc ggtgaccctc acctggcagg
     5401 cacctataac tggaacacct acaggataca aggtctataa gaacaacacc gccctcacca
     5461 ctgtgaccgg actgacttac acagatacgg ctgtgaccga tggcacaacc tacagctaca
     5521 aggtaaccgc gctctacggc a
//
LOCUS       DAOOWR010000087         5536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_14815_length_5536_cov_3.055647, whole genome shotgun sequence.
ACCESSION   DAOOWR010000087 DAOOWR010000000
VERSION     DAOOWR010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14815_length_5536_cov_3.055647"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2698)
                     /locus_tag="PLB85_03180"
     CDS             complement(<1..2698)
                     /locus_tag="PLB85_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72361.1"
                     /translation="MKLRYILLLLLLLAAGASPAYYFGQNKVNLVPEEWSTLQTMHFD
                     IYYPRGEDEFGRVVALMAEETYYTLKDQLTFPIGSRIPVMFYSSKTNFQSTNIIYPLL
                     TEGVGGFTESLRNRVAIPFDGSYSDLEELLAHELTHAYTNALEDGVANALRSMRPTSF
                     PFWFSEGLPEYLSIGGESDYNNMFILDMVVNDKLPPLQNLGGYYAYRLGESFLAYLAR
                     SYGKDKVTEYYYTLRSSNGLDNATKKVFGLEFDDLESRWRYQLKRDFYPLVESQGIPV
                     VNLEKRSDSKKKGAYFNLMPRFSPDGSRYVWFSSAESRYSVWMGSLHGLSEPRRILKG
                     EATGKIEEFHYFRSNLSWFPDNRRVAFAAKTANGDRIHILDVDRRKIVRTIAPAGMSA
                     IYELDVAPDGRSLVFAGQQGMRCDLFLMDMESENLTRITDDLYNDLQPRFTPDGQSVV
                     FASERSRDPESRRRGLFANLNRNIFSLDLAGGELTQHTFEKKDCSFPQVDAKGQNLYY
                     INSDGGVSNIFALDLKGGGRAKVTSVLSGVYSADLSPDSRHLLLANYFDGAWDIYFKD
                     RSLDTLCFSAYPEPRTVALEDSLLAGIDLGELDRYGKREAVKPRRVNPAAGFATRDPF
                     LTEFPTFEYTAEDSLLLQRDFSYDDRPQTKDNPPVVKPYRARFSLDSLWGGLAYSPAV
                     GTIGYVELSMSDMMGDHGIGINAGVSGKLEESNLALTYLYLRQRMDYGIGVFNLNDEY
                     FFREVVPGPNNDIWYRYRERQSGGYFLLRYPFSRFLRLEFDQMVYQRGSYLYQLGDSL
                     EVELASDLDLVYSPGFRLVHDNALYGSTGPLLGWRLYYDLNTTINDGKIDYVTNYLDW
                     RSYTLFSKRYSIALRGIAGISTGPNHQRFSLGGYYGIRAYGDN"
     gene            complement(2707..3609)
                     /locus_tag="PLB85_03185"
     CDS             complement(2707..3609)
                     /locus_tag="PLB85_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOU72362.1"
                     /translation="MTRFYPNIRKTQLKVGLFTILTLAILLFCYLWLSNRISTRAQTD
                     LRISFDEVMGLEVGDQVTFRGMEVGRVKKIEARDRDILVTARINSDVRLKEGSRFLIS
                     DSSLMGGTLLNIAPGEGPGLLNTKQVLEGDSPQGIMGVIGEATLAIEEIKTLFASLRG
                     EDGLIGKSNLVLDNAQQAATSVDRLALEAKGDLEAALGQIERLTGEVRNVVQVSSADL
                     EGALGKAPEAMARVNSALDSLKILSGRLDATLHTLNSGQGTAGKLLRDDGLYRQFQSS
                     VASLDSLVADVRANPRKYLKFSIF"
     gene            complement(3619..4866)
                     /locus_tag="PLB85_03190"
     CDS             complement(3619..4866)
                     /locus_tag="PLB85_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOU72363.1"
                     /translation="MKRYLLILVVALCSLLGAEILVPMDQTQGNHLKAYGVAFEALKE
                     QYVVKWLLNYRGGSFLMPESEGLAALCNIRGVRFELVSSAQVAAIMAEIQDANMEALV
                     LEKEPKIAIYIPPNALPWDDAVTLALNYAEIDYDRFWDDEVLDGKLVDYDWLHLHHED
                     FTGQFGKFYGSYRHMPWYQNDVQVNEAMAARHGYAKVWQLKHAVAQKIREFVENGGFL
                     FAMCAATDTFDIAMAAYGTDIVDSLIDGDGIDPQYQSKLDFSRCFAFENFKLKTNPFE
                     YEFSDIDASDYSRLRGAEADYFQLFDFSAKYDPVPTMLTQCHVNVIDGFLGQTTSFFK
                     DKVKKSVIILAEVPGQNEVKYIHGNIGKGTFTFYGGHDPEDYQHKVGDPETILDLHKN
                     SPGYRLILNNILFPAAEKKELKT"
BASE COUNT         1152 a         1513 c         1610 g         1261 t
ORIGIN      
        1 ggttgtcccc gtaagcccgg atgccgtaat atccgcccag ggaaaagcgc tggtgattcg
       61 gccccgtgct gattccggcg atacctctga gggcaatgga atagcgcttg ctgaagaggg
      121 tgtagctgcg ccagtccaga tagttggtca catagtcgat cttgccgtcg ttgatggtgg
      181 tgttcaggtc gtaatagagc cgccagccaa gcaaagggcc tgtggaaccg tagagggcgt
      241 tgtcgtgcac cagtctgaaa ccgggtgaat aaacgaggtc gaggtcgctg gccagttcca
      301 cttcgaggct gtcgccaagc tggtagaggt agctgccgcg ctggtaaacc atttggtcga
      361 attccaatct caaaaatcgg ctgaaaggat agcgcagcag aaagtagccg ccgctctggc
      421 gttcccggta gcgataccag atgtcgttgt tgggtcccgg cacgacctct ctgaaaaagt
      481 actcatcgtt gaggttgaag acgccgattc cgtaatccat ccgctgcctc agatagagat
      541 aggtgagcgc gaggtttgat tcctccagct ttccggacac tccggcgttg atgccgatgc
      601 cgtggtcgcc catcatatcg ctcatgctca gttccacata gccaatggta cccaccgcgg
      661 gcgaataggc cagtccgccc cagaggctgt ccagggaaaa ccttgcccgg tatggcttta
      721 ccactggcgg gttgtcctta gtttggggac ggtcatcgta gctgaagtcc cgctgcagca
      781 gcagagagtc ctcggcggtg tattcgaaag tgggaaattc agttaggaaa gggtccctgg
      841 tggcgaaacc ggcggcggga ttgacccggc ggggcttcac cgcttcccgt ttgccgtagc
      901 ggtccaattc gcccaggtcg atgccagcca gcaggctgtc ttccagcgcg acggtccggg
      961 gttctggata ggcggagaaa caaagcgtat ccaagcttct atccttgaaa tagatatccc
     1021 aggcgccgtc gaaatagttg gccagcaaca gatggcggct gtcgggggaa aggtcagcgg
     1081 aatagactcc ggagaggacg ctcgtcactt ttgccctgcc tccgcccttc aggtccagcg
     1141 cgaagatatt gctcacgccg ccatcggagt tgatatagta aaggttttgg cctttggcgt
     1201 ccacctgggg gaagctgcag tctttttttt caaaggtgtg ttgggtcagt tctccgccgg
     1261 caaggtcaag gctgaagatg ttccggttga ggttggcaaa aaggccccgg cgcctgcttt
     1321 ccggatcgcg gctgcgttcc gaggcgaaga ccacgctctg cccgtccggg gtgaagcggg
     1381 gctggaggtc gttgtagagg tcatcagtga tgcgtgtgag gttttcgctc tccatatcca
     1441 tcagaaaaag gtcacagcgc atcccctgct gtccggcgaa aaccaggctg cggccgtccg
     1501 gagccacgtc cagttcatag atggcgctca tcccggcggg ggcaatggtg cgcacgatct
     1561 tgcgtctgtc aacgtccagg atgtggatac ggtcgccatt ggcggttttg gcggcaaagg
     1621 ccacgcggcg gttgtccggg aaccagctca ggttggagcg gaagtagtga aattcctcta
     1681 ttttcccggt ggcttcgcct ttcaggattc ggcgcggctc gctcaggccg tggaggctgc
     1741 ccatccaaac gctgtagcga gattcggcgc tgctgaacca gacatagcgg ctgccatcag
     1801 gcgaaaagcg gggcatcagg ttgaaataag cgcccttttt cttgctgtcg ctgcgttttt
     1861 ccaagttcac cacagggatg ccctggcttt ccaccagggg gtaaaaatca cgtttgagct
     1921 ggtagcgcca gcgggattca aggtcatcga actccagacc gaaaaccttc ttggtggcgt
     1981 tgtccagacc gtttgaagaa cgcagagtgt aataatattc cgtcaccttg tctttgccat
     2041 agctcctggc gaggtaggct agaaagcttt cccccagccg gtaggcgtag tatccgccca
     2101 ggttttgcag agggggcagc ttgtcgttca ccaccatgtc caggatgaac atgttgttgt
     2161 aatcgctctc tccgccgatg gagaggtatt ccggcaaacc ttccgaaaac cagaagggaa
     2221 aagaggtggg gcgcatggac ctgagcgcgt tagcgacccc gtcttccaag gcgttggtgt
     2281 aagcgtgggt aagctcgtgg gccagcagtt cctccaggtc ggaatagctt ccgtcgaaag
     2341 gtattgccac ccggttgcgc aggctttcgg tgaagccgcc aaccccttcc gtgagcaggg
     2401 gatagatgat gtttgtgctt tgaaaatttg tcttgctgct gtagaacatc accgggatgc
     2461 gggaaccgat gggaaaagtg agctggtctt tcagcgtgta gtaggtctcc tccgccatca
     2521 gtgccaccac ccggccgaat tcatcttcgc cccggggata atagatgtcg aagtgcatgg
     2581 tctgcagggt ggaccactcc tcggggacaa ggttgacctt gttttgcccg aagtaatagg
     2641 cgggggaggc tcccgccgcc agcagcagca agagcaggag aatgtatctc agtttcatcg
     2701 gcatccttag aatatgctga atttaaggta tttcctgggg ttggcacgaa cgtccgccac
     2761 tagcgaatcc agactggcca ctgagctttg gaactggcgg taaaggccgt cgtcgcgcag
     2821 cagctttccg gccgtgccct gtccgctgtt cagggtgtgc aaagtcgcgt ccagcctgcc
     2881 cgacaggatt ttcagactgt ccagggcgga gtttaccctg gccatggctt ccggcgcctt
     2941 gccgagagcg ccttccaaat cggctgaact gacctggaca acattccgca cctcgccggt
     3001 gagcctttcg atttgcccca acgcggcttc caaatcgcct ttggcctcca aagccagcct
     3061 gtccaccgac gtcgccgctt gctgggcatt gtcgagcacc aggttcgatt tgccgatcaa
     3121 tccgtcttcc ccacgcagac tggcgaaaag ggtttttatc tcttcgatgg ccaaagtggc
     3181 ttcaccgatg acgcccatta tcccctgggg tgaatcaccc tcgagaacct gcttggtgtt
     3241 caaaaggccc ggtccctctc ctggagcgat gttgagcagg gtgcccccca tcaggctgct
     3301 gtctgaaatc aggaagcggg agccctcttt gaggcggacg tcgctgttta tccgcgccgt
     3361 caccagaata tcccggtccc gcgcctctat ctttttcacc cggcccactt ccatgcccct
     3421 gaaagtcacc tggtccccta cctccaggcc catcacctcg tcaaagctga tgcgcaggtc
     3481 cgtctgggcg cgggtcgaga tgcggttgct caaccacagg tagcagaaca acagaatagc
     3541 cagggttaga atcgtgaaga ggcccacctt gagctgggtt ttgcggatgt tcggatagaa
     3601 tcttgtcacg gcccttcctc aggttttcag ttctttcttc tcggcggccg gaaagaggat
     3661 gttgttcagg atcagcctgt atccgggcga gtttttatga aggtcgagga tggtttcggg
     3721 atcgccgacc ttgtgctggt aatcctcggg atcgtgtccg ccgtagaagg tgaaggtgcc
     3781 tttgccgatg ttgccgtgga tgtatttaac ctcattctgg cccggcacct cggccaggat
     3841 gatcacgctt ttcttcacct tgtccttgaa aaaggaggtg gtttgcccca gaaagccgtc
     3901 gataacgttc acgtggcact gggtgagcat ggtgggcacg ggatcgtatt tggctgaaaa
     3961 atcgaatagt tggaaatagt ccgcttccgc cccgcgcagc cgggaatagt cgctggcgtc
     4021 gatatcggaa aattcgtatt cgaaaggatt ggtctttagc ttgaagtttt cgaaggcaaa
     4081 gcagcggctg aaatccagct tgctttggta ttgggggtcg attccgtcgc cgtcgatgag
     4141 gctgtccaca atgtccgttc cgtaagccgc catcgcgatg tcgaaggtat ccgtggcggc
     4201 acacatggca aagaggaatc cgccattctc cacaaactcg cggatttttt gcgccaccgc
     4261 gtgcttaagc tgccaaacct tggcgtagcc gtgcctcgcg gccattgcct cgttcacctg
     4321 gacgtcgttt tgataccagg gcatgtggcg gtaggaaccg taaaacttgc cgaactgccc
     4381 cgtgaaatcc tcatgatgca gatgcagcca gtcataatcc accagcttgc catccagcac
     4441 ctcatcgtcc cagaaacggt cataatcgat ttcggcgtag ttcagggcca gggtcaccgc
     4501 gtcgtcccaa ggcaaggcgt tgggcgggat gtagatggcg attttcggct ctttttccag
     4561 aaccagcgcc tccatgttcg catcctggat ttcggccatg atggcggcca cttgggcgga
     4621 ggataccagc tcgaaacgca ctccgcggat gttgcagagc gcggccaggc cttcggattc
     4681 cggcatcagg aaacttccgc cccggtaatt gagcagccat ttcaccacat actgctcttt
     4741 aagcgcttcg aaggcgactc cgtaggcttt caggtggttg ccctgggtct ggtccatcgg
     4801 caccaggatt tcagccccca acagggagca cagcgcaacc accaaaatca gcaagtatct
     4861 tttcacgcgg ttcctcgttc ggcgtatcat ccctttggta aagggatttt cacccatttt
     4921 tccatgttaa atatcaaaac cctccagcgg ccataaatct gtcaagctaa ttctgccaga
     4981 gctttgtcgc actatagctt tctgaggaca tgctccccca aggttgatgc ctgaaccaga
     5041 aatgaggcag cccccagcat agacagccac cataccgctt ttcttttctc aacggattca
     5101 actgatttgg ggagaaaaag ttggcgtaga gctccttggt catcctgatt tccgaactcc
     5161 aacagagcct cgcagaggca gcaggacgat agcgagggtg tgaagtggcc gggaacccga
     5221 tgaaagaatg aagcgatgct gtctggccgt taaggccctt cattgtgcta cggattcgcc
     5281 tcccgcacaa tgcccgcatt tgattcgagg atcttgcggg aggcgtggga gaggaatggt
     5341 cgcgagcgct cagggcctgc ctgacaggac attgctatgg gtaagcaggt attgctctct
     5401 tttagactaa ggcgctcaat cagaaagtga ctctccgggc tttaccaacc aagcatggaa
     5461 gagaaccaaa aagggaaaag gtaggtgata gaaatgaagc ggcagacatt accaaccagg
     5521 aagatcgacg ttcccg
//
LOCUS       DAOOWR010000088         5455 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_15079_length_5455_cov_2.856481, whole genome shotgun sequence.
ACCESSION   DAOOWR010000088 DAOOWR010000000
VERSION     DAOOWR010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15079_length_5455_cov_2.856481"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLB85_03195"
     CDS             complement(<1..508)
                     /locus_tag="PLB85_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72364.1"
                     /translation="MKHTLLLVLALAFCASAVAVDFRVPHLLVHETAIQPYNIDFQED
                     QVILRNQNFIWIHSIFNPWSPRLEAVFRSQYIIEDVNMLGGNQIYICSQEPANLVSTV
                     DTLSYPGTIYFTNTVPGNKITREGSMLYVADRFKGIDIIDIGKGGMRELKSTFSEKWG
                     VRDFIAEYP"
     gene            complement(584..1564)
                     /locus_tag="PLB85_03200"
     CDS             complement(584..1564)
                     /locus_tag="PLB85_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU72365.1"
                     /translation="MNSEAMIGKIEELKSVKQELLAEIGKVIVGQDDVVEKVLMGLFA
                     NGHMLIEGVPGLAKTLLISTIAKALNLSFGRIQFTPDLMPSDITGSDILVSLSGGERR
                     EFQYIKGPIFANIILADEINRTPPKTQAALLQAMQEYQVSSGNVTRPLDLPFIVMATQ
                     NPIEQEGTYPLPEAQLDRFMLYINIGYPSYEEELTIVQNTTGAAAEEPRPLLSAERII
                     AFQSAIRAMPLSSHVLEFAVKLARLTRPGNPELDPGLAKWINWGAGPRASQYLVLGAK
                     ARAALDGRLTPSEEDVRAVALPVLTHRVLISFAAEAEGMTSADIIRQLIK"
     gene            complement(1554..2582)
                     /locus_tag="PLB85_03205"
     CDS             complement(1554..2582)
                     /locus_tag="PLB85_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU72366.1"
                     /translation="MSAKPVLMSRKRIAFLIAGFLLGLALIWLWLRSIDTSAVLDSIR
                     RVKPDLVAFAAIAYLSAYFIRSWRWNLLLRSQTAISLGRTFLYSMGGNWVNYLIPIRA
                     GELVKAWFVKHNHGLPLLNVLPSIFIDKTFDTLGILFVLVMVPLLRIRVSVGLTVLLV
                     LLGLVFLVSLGILLLAVWRKDGVVRFLQTFFAWLPRRWREKIFGYIDVFIRGLNVFEQ
                     HWSRLVVAVLLTALGVGLDGLYFHLLFRAFGVEFPFVYVLFGYTLINLSYALPQPPAQ
                     LGSNEWMMIIIFSVGFALTKDSASAIMAFAHVLTAILIGVIGFAAIAASGSQVLKAII
                     RGDKIYEQ"
     gene            complement(2572..3420)
                     /locus_tag="PLB85_03210"
     CDS             complement(2572..3420)
                     /locus_tag="PLB85_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU72367.1"
                     /translation="MAIPIINDWEKFFDSPHEGLGSSYERIVLNELLLATANKYEARN
                     VLESPSFGFTGISGINLFALADSGLEVTLEDDNPRRLELIRNLWHTMDRSFTARHNPD
                     FRRLDYPDRAFDLSFSFSALWFVPDLKAFLSELSRVTAKAIFISVPNRSGLGYKMQLK
                     DYSAEKYPDLRLSNIDPPSIIRLLKNLGWKLAESGCFDCPPWPDIGMTKEELLAKWLP
                     GRLLPKKLSQPKEEEGETLSILSYYSGDDPGFADRMRSYQWLENAAPEALKRVWAHHF
                     RMVFER"
     gene            complement(3420..4394)
                     /locus_tag="PLB85_03215"
     CDS             complement(3420..4394)
                     /locus_tag="PLB85_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU72368.1"
                     /translation="MPRLLITGVTGLIGRAALNNVLAAKTDYHVTALIRPETQPERYS
                     AFRTELEIVKMDLADTDKLTAWLKDNAFDAVPHIGALRGGRKFSHQEFLRANFTSTQS
                     LAEHCLLHNAKFMFCSSVGVFGAIPEELPANNQTERNPDNFYHYTKIESEKMIQRMVV
                     RGLKAAILRPSVTYGTRDRGFPYQLVKMVHKNRFPLINKRVWLHLCHVDTISRAFTWL
                     LLNDYKPGLALNVADREPVELQQLVNFISRELKGDNYPSILKLDRIFFRLGEQAAKLV
                     NNELWVSRFQLISRSWFYHVADTYELMGLPPTFTIPDIRITIEDYLKK"
     gene            complement(4395..5186)
                     /locus_tag="PLB85_03220"
     CDS             complement(4395..5186)
                     /locus_tag="PLB85_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOU72369.1"
                     /translation="MIDIHCHLLPQTDDGSDSEEKSIAQLKLMAEGGVTDVFMTSHFM
                     PGFYQYPRADYDAKLARLRELASGEGLGIRLHPGFEIFLHPFSLKDIEKHDLRMGNSR
                     YILVESDLNGLPSDFYEYLYPLLRKGYKPILAHAERYVSIMRNVHDAKDLIRRDIYMQ
                     VNAGSLLGNYGEKVRQTAWLLLRNGWAHFLASDDHAKTPYGSYFQALELLRTELDPTV
                     AELLSIDFPAKIIADETVPFKYVYLRPSHERRHRKKRSWFQRIFG"
     gene            complement(5190..>5455)
                     /locus_tag="PLB85_03225"
     CDS             complement(5190..>5455)
                     /locus_tag="PLB85_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="geranyl transferase"
                     /protein_id="HOU72370.1"
                     /translation="SGYALNLGLAYQMIEDINKDYKHGSEDIDFDEDYVPASKRTYTG
                     FLGFDKARLAVEKMLDESLRMISPFEHTDVLVEFVQMIKENLP"
BASE COUNT         1198 a         1399 c         1668 g         1190 t
ORIGIN      
        1 agggatattc ggcgatgaaa tcgcggacgc cccacttttc ggaaaaggtg cttttaagct
       61 cgcgcatccc gcctttgccg atgtctatga tgtcgattcc tttgaagcgg tcggctacat
      121 agagcatcga gccttcgcgg gtgatcttgt tgccgggcac ggtgttcgtg aaatagatgg
      181 tgccggggta ggaaagggtg tcaacggtgg aaacgaggtt ggcgggttcc tggctacaga
      241 tgtagatctg gtttccgcca agcatgttca cgtcctcaat gatgtattgg gagcggaaaa
      301 cggcctcgag gcgcggcgac caggggttga aaatggaatg tatccagata aagttctggt
      361 tgcggaggat aacctggtct tcctggaagt cgatattgta tggctggatc gcggtttcgt
      421 ggaccagcag atgcgggacg cggaaatcga cggcaaccgc ggacgcgcag aaggcaaggg
      481 ccaaaacaag cagcagagtg tgtttcattt gtcactcctt attggatgtg aggcagcttt
      541 ggaggctgcg cagtatatac aaggcaggcg ggcagttcct gcatcatttg atcagttggc
      601 ggatgatatc agccgaagtc atcccctcag cctcagcggc aaaggatatc agcaccctgt
      661 gggtgagcac gggcagcgcc acagccctca catcctcttc cgaaggggtg aggcggccgt
      721 ccaaggcggc tctggctttg gcacccaaaa ccagatactg gctggcacgg gggccggcac
      781 cccagttgat ccatttggct aggccggggt caagctcggg attcccgggg cgggtgaggc
      841 gggccagctt caccgcgaat tccagaacgt gggaactgag cggcatggcg cggatggcgg
      901 actggaaggc gattatccgc tccgccgaaa gaagggggcg cggctcttcc gcagcggcgc
      961 cggtggtgtt ttgcacgatg gtcagctcct cctcatagga gggatagccg atgttgatgt
     1021 agagcatgaa gcggtcgagc tgggcttccg gcagaggata ggttccttcc tgttcgatgg
     1081 ggttttgggt tgccatcacg atgaacggca aatccagcgg acgggtcacg tttccgctgc
     1141 tcacctggta ttcctgcatc gcctggagca gggcagcctg ggttttgggc ggggtgcggt
     1201 tgatctcgtc ggccaggatg atattggcaa agattgggcc cttgatatat tgaaattccc
     1261 ggcgctcgcc gccgctgagg gagaccagga tgtccgaacc cgtgatgtcc gaaggcatta
     1321 ggtccggagt gaactggatg cggccaaagg agaggttgag ggctttggcg atggtcgaga
     1381 tgagcagggt tttagccagg ccgggcacgc cttcgatgag catatggccg ttggcgaaga
     1441 ggcccatcag cactttttcc accacgtcgt cctggcccac gatgaccttg ccgatttcgg
     1501 ccagcagctc ctgcttcacg cttttcaatt cttcgatctt gccgatcatg gcttcactgt
     1561 tcatatattt tgtctccacg gataatcgct ttcagcactt ggcttccgct cgcggcgatg
     1621 gcggcaaagc cgatcacgcc gatgagtatg gccgtcaaaa cgtgcgcgaa agccatgatc
     1681 gccgaagcgc tgtcttttgt caatgcaaat ccaaccgaga aaatgattat catcatccat
     1741 tcgttgctgc ccagttgggc cgggggctgg ggcagggcgt aggaaaggtt gatcagggta
     1801 tagccgaaaa gcacgtaaac gaaaggaaat tccaccccga aagcccggaa gaggagatgg
     1861 aaatagaggc cgtccaggcc cactccaagg gcggtgagca gcacggcgac aaccaatctt
     1921 gaccagtgct gctcaaacac gttcaagccg cggatgaaaa cgtcgatgta gccgaagatt
     1981 ttctcccgcc agcgtcgggg cagccaggcg aagaaggttt gcaaaaacct caccacgcca
     2041 tcctttcgcc acaccgccag cagcagaatc cccagggaaa ccaggaacac caggcccagc
     2101 agcaccagca gcacagtgag gccgacagac acgcggattc tcagtagcgg aaccatcacc
     2161 agcacgaaga ggatgcccag ggtgtcgaaa gtcttgtcaa taaagatgga gggcagcaca
     2221 ttgagcaggg gcaggccgtg attgtgtttc acaaaccagg ccttcaccag ctctccggcc
     2281 ctgatgggga tgaggtagtt gacccagttt ccgcccatgc tgtagagaaa ggtgcggccc
     2341 agggaaatgg cggtttggga tctcaacagc aggttccaac gccaactgcg gatgaaatag
     2401 gcgctcaggt aggcgatggc ggcgaaggcc accaaatcgg gttttaccct tcggatgctg
     2461 tccagcacag cgcttgtgtc gatgctgcgc agccagagcc agatcagcgc cagtcccaga
     2521 agaaagccgg cgatgagaaa agcaatgcgc ttgcgggaca tcaggaccgg tttagcgctc
     2581 aaagaccatc ctgaaatggt gggcccaaac ccgcttcagg gcttccggcg cggcattttc
     2641 cagccactgg tagctccgca tccgatcggc aaaaccggga tcgtcgccag aataataact
     2701 gaggatgctc agggtctcgc cctcctcttc ctttggctgt gacagctttt taggcagcag
     2761 tctccccggc agccatttgg ccagaagttc ctccttggtc atgccgatat ccggccaggg
     2821 cgggcagtcg aaacagccgg actcggccag tttccagccc aggtttttga gcaggcggat
     2881 gatcgacggc gggtcgatgt tgctcaggcg gaggtcagga tacttttcag cgctataatc
     2941 cttcaattgc atcttgtagc ccaggccgga gcggtttggc acgctgatga agatggcttt
     3001 cgcggtcacg cgggaaagtt cactcaaaaa ggctttcagg tccggcacga accagagcgc
     3061 ggaaaaggaa aaactgaggt cgaaagcccg gtcgggataa tccaggcggc ggaaatccgg
     3121 attgtggcgg gccgtgaaag agcggtccat cgtgtgccag agattgcgta tcagttcaag
     3181 gcggcgggga ttgtcatcct ccagggtcac ttccaggccg gaatcggcca gggcgaagag
     3241 gttgatcccg ctgataccgg tgaagccgaa ggaagggctt tccagcacat tccgggcttc
     3301 atacttgttg gcggtggcca gcagcagttc gttcagcacg atgcgttcgt aggaagagcc
     3361 caaaccctca tgggggctgt caaagaattt ttcccagtcg ttgatgatcg gaatggccat
     3421 tatttcttca gatagtcttc aatggtgatc cggatatccg gaatggtgaa agtggggggg
     3481 aggcccatca gttcgtaggt atcggccacg tgatagaacc aggagcggga tatcagttga
     3541 aagcggctga cccaaagctc gttgttgacg agtttcgccg cctgttcacc caagcggaag
     3601 aaaatccggt ccagcttgag gatggagggg tagttatcgc ctttcaactc ccgggaaatg
     3661 aagttcacca gttgctgcag ctccaccggc tcgcggtcgg ccacattcag tgccagaccc
     3721 ggtttgtagt cgttcagcag cagccaggtg aaggcgcggg aaatggtgtc cacgtggcag
     3781 agatgcagcc agacgcgttt attgatgagg gggaagcggt tcttgtgcac catcttcacc
     3841 aactggtatg ggaagccgcg gtcgcgggtg ccgtaggtca cggaagggcg caaaatggcg
     3901 gctttcaggc ctcgcacgac catccgctgg atcattttct cgctttcgat cttggtgtag
     3961 tggtaaaaat tatccgggtt acgttctgtc tgattgttcg ccggcaattc ctccgggatg
     4021 gcgccgaaga ccccaaccga ggaacagaac atgaatttgg cgttgtgcag aaggcagtgt
     4081 tccgccaggg actgggtaga ggtgaagttg gcccgcagaa attcctggtg gctgaatttt
     4141 cggccgcccc gtagcgcgcc tatgtgcggc acggcgtcga aagcgttgtc tttcagccag
     4201 gctgtgagtt tgtctgtgtc cgccagatcc atttttacga tttccagttc ggtgcggaag
     4261 gcggaataac gctctggctg agtttcaggg cggataagcg cggtgacgtg gtaatccgtt
     4321 ttagcggcca gaacattgtt cagggccgcc cgcccgatga gtccggtaac gcctgtgatc
     4381 agcaatctgg gcatttatcc aaaaatcctt tggaaccagc ttctcttctt gcggtggcgc
     4441 cgttcgtggc tgggacgcag ataaacgtat ttgaaaggaa cggtttcgtc ggcaatgatt
     4501 ttggcgggaa aatctatgga gagcaattca gcaacggtgg ggtccagctc cgtcctaagc
     4561 aattccaagg cttggaaata cgatccgtag ggcgttttgg cgtggtcatc agaagccagg
     4621 aagtgggccc agccgttgcg cagcagcagc caggcggtct ggcgaacctt ttcaccgtag
     4681 tttcccaaaa ggctgccggc gttcacctgc atgtaaatgt cacgccggat gaggtccttg
     4741 gcgtcatgga cgtttctcat gatgctcaca tagcgttctg cgtgggccag gatgggcttg
     4801 tagcctttgc ggagcagggg gtagaggtat tcgtaaaaat cactgggcag gccgttgagg
     4861 tcggattcaa ccaggatgta gcgtgagttt cccatcctaa ggtcgtgttt ctcaatgtct
     4921 ttcaggctga aaggatgcag gaagatttca aagcccggat gcagcctgat ccccaagcct
     4981 tcgccagacg ccagttcacg cagcctggca agtttggcgt cgtaatccgc acgcggatac
     5041 tgatagaagc ccggcatgaa atgggaggtc ataaaaacgt ctgtgacgcc accctcagcc
     5101 atcagtttca gctgggcaat cgatttttcc tcagaatcag agccgtcatc ggtctgaggc
     5161 aacagatggc aatgaatgtc gatcatctct cagggtagat tctcctttat catttgcaca
     5221 aactctacca gaacatccgt atgttcgaaa gggcttatca tccgcaaact ttcgtccagc
     5281 atcttctcca ccgccaggcg ggctttgtcg aaacccagga agccggtgta ggttcttttt
     5341 gaggcgggga cgtaatcttc gtcgaaatct atgtcctcgc tgccgtgttt ataatctttg
     5401 tttatatctt ctatcatctg ataggcaagg ccaaggttca gggcatagcc gctca
//
LOCUS       DAOOWR010000089         5415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_15217_length_5415_cov_3.792351, whole genome shotgun sequence.
ACCESSION   DAOOWR010000089 DAOOWR010000000
VERSION     DAOOWR010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15217_length_5415_cov_3.792351"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLB85_03230"
     CDS             complement(<1..685)
                     /locus_tag="PLB85_03230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU72371.1"
                     /translation="MKHKFVMPLLMLLLAGNLSALHFARWSTSMGSFTAEIYDQWVPI
                     TANNFISLANSGFYNNLIFHRVVAGFVIQDGCPLGTGYGGPGYTIPDEFHPNLHHDQA
                     GILAMARTSAPNSAGSQYYITLAPTPHLDGNYAIFGKVVSGLDTVLAIGQVPVGANDR
                     PLTPVTIDTLRILDLAIMNVTPSDSTTVTVNVNELQTFVVESISSLPTQFDWYVDGEP
                     VVGMDFILEA"
     gene            865..2859
                     /gene="asnB"
                     /locus_tag="PLB85_03235"
     CDS             865..2859
                     /gene="asnB"
                     /locus_tag="PLB85_03235"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOU72372.1"
                     /translation="MSGISGIYCLNPESKAELRLLKEMTSTIRHRGPDDEGYFLCCPE
                     NGFKALYSGEESTADVKAVLPRLDITAKARLGLGFRRLATLDLEASGHQPMSDPELGL
                     HIVFNGNIYNHMELREELKQAGYSFRSRSDTEVILKAYHAWGDDCLRHFNGIWALAIW
                     DEKQRRLFCARDRFGVKPFYYCIHDQVLYFGSEIKQLLLTPVNKSLNRPMLWRAMKIN
                     SLQVYGAETFWNNIHALEPGTTLSVQDGQISARTYHHLDPSIFGTSTLSLNDATDQYR
                     KLFQRAVGWQMRSDVEVGACLSGGLDSSAIVCSATQLSETPLQTFSSYFAELPQLDER
                     RWIAEVASACGCVSHLCSPEVDDALCWFAEATWFNDLPLGSGFAAQYSVMRLAHEYGV
                     KVLLDGQGSDELTAGYKHAQYRWLADLLLDQQQGRFWPELKAYLASNPIPKGLSGIAK
                     TLLAKALPESRLYALEFRHLRFEPFNREFTSQARNSLESGILARLEDIEGTKLANFLY
                     NEIYFTSLPTLLHWADRMSMAASVESRVPFLDHELVEFAFSLPSSHKVHQATGKRIHR
                     QAMRSIVPKAIAERRDKAVFGSPFHALWMRGGMKSAVEELFSSTEFRCRGIWDLPRIW
                     TNWQKYKKGDNSQAEMIFNVFALENWFREFGPWIDYDQQT"
     gene            2880..3779
                     /locus_tag="PLB85_03240"
     CDS             2880..3779
                     /locus_tag="PLB85_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72373.1"
                     /translation="MRKALYCMLICLAMLGLGSCKWFFKSPKVEQIHDIKVVSIDPDK
                     IRLEISLSVLNPNRYKLKLNDMDLILLNKNREQIGTASLNRAVEIPGRKANALGFDIT
                     LDTRPTVKLVNYSDQTVFVYIAGRGSGKVLGTRQSLVFEEPYEIDIRSQLQKVLGTFK
                     ANGDDIFKIKRSYLGKVGITETQILVDFIVMNPYGLKFTLADFPATITIGNAESGSGN
                     LDKALNFDEKVYSREGTMIFKVNNLKAIYNIAKGAINGEVSYRVDGEVKINAYGAEMK
                     QSYSYKDLITISIADEIIKLLVQ"
     gene            complement(3927..4193)
                     /locus_tag="PLB85_03245"
     CDS             complement(3927..4193)
                     /locus_tag="PLB85_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72374.1"
                     /translation="MLYSLPDGRSFELEQVSRVSRIRDEGMGFGPVDYSKLTFRISLR
                     VNEVVEVTLLYLYSDWVEQKKKLASIRADLLKKLRDNGINVDDS"
     gene            4749..5039
                     /locus_tag="PLB85_03250"
     CDS             4749..5039
                     /locus_tag="PLB85_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72375.1"
                     /translation="MKRKLLLILSLLACLALLCSCAPGIGGRSAENRAGFFMGIWHGW
                     IAPFSLIWHWFNRDVRLYEVFNTGWAYDFGFYMGVVGGFGSLALSRKRRRDD"
     gene            5058..>5415
                     /locus_tag="PLB85_03255"
     CDS             5058..>5415
                     /locus_tag="PLB85_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU72376.1"
                     /translation="MAAYLTLVLTAAIWGLAFAAQRKGMESLDPFTFNALRFALGAAC
                     IALTGLISKAKKPNPEIPVTDHTPSNGKSWKGPLLLGLMLFLASSFQQFGMLWTGAGS
                     AGFITGLYVVFVPLVGL"
BASE COUNT         1310 a         1461 c         1368 g         1276 t
ORIGIN      
        1 cggcttccaa tatgaaatcc atacccacga ccggctctcc gtccacatac cagtcaaact
       61 gggtcggaag gctgctaatc gattccacta caaacgtctg cagttcgtta acgttgaccg
      121 taaccgtggt ggaatccgat ggcgtcacgt tcatgatggc caaatccagt atcctcaaag
      181 tatcgatcgt tacaggcgtt agtggtctat cattggcgcc aactggaacc tgcccaatcg
      241 ccagaactgt gtccaagcct gaaacaacct tgccaaaaat ggcgtagttg ccgtccagat
      301 gcggggttgg ggccaatgtg atgtaatact gggatcctgc tgagttcgga gcgctggtac
      361 gtgccatggc caaaatcccg gcttggtcgt gatgcaggtt gggatgaaat tcgtcgggaa
      421 tggtgtagcc aggcccgcca tagccggtgc ccaggggaca gccgtcctgg atcacgaaac
      481 ccgccaccac ccggtggaag atcagattgt tgtaaaagcc actgttggcc agagaaatga
      541 agttgttggc ggtgatgggc acccactggt cgtagatttc ggcagtaaaa gagcccatgg
      601 aagtgctcca gcgggcaaaa tgcaaagcgc tgaggtttcc cgccagcagc aacatcagca
      661 atggcatcac aaacttgtgt ttcatgttca gcctcactat tcttggtttg agagtcgtca
      721 acttcctgca aagccttgcc ggaagccatt caaacctgct tgaatggaca tctggttcct
      781 gtcaatagat ttatccccct atcatagaga tctgctaaaa ttagcttgac catattctga
      841 ggttcagtta tctgcttcat catcatgagt ggaattagcg gaatctattg tctcaatccg
      901 gaaagtaaag ccgaactccg gctgctaaag gaaatgacct ccacaatcag gcaccgcggg
      961 cctgatgacg agggatattt tctctgctgc cccgaaaacg gtttcaaggc tttatacagc
     1021 ggagaagaat ccacagcgga tgtgaaggct gtactccccc ggctcgatat tacggcaaaa
     1081 gcccgtctgg gacttggttt ccggcgcctt gccaccttag atctggaggc cagcggtcac
     1141 cagcctatga gcgatccgga gttaggcctg cacatcgttt tcaacggcaa tatctacaac
     1201 cacatggagc tgcgggaaga gctgaagcag gctggatact cattccgcag ccgttccgac
     1261 accgaggtga tcctcaaagc ctaccacgcc tggggcgatg attgcctgag gcatttcaac
     1321 ggcatctggg cgctggccat ctgggacgaa aaacaacgcc ggctattctg cgctagggac
     1381 cgcttcggag taaaaccctt ctattactgc atccatgacc aagtgctcta tttcggctca
     1441 gagataaagc aactgctgct tactcccgtg aacaaaagcc tgaacaggcc gatgctttgg
     1501 cgggcgatga agataaattc gctacaggtg tatggtgctg agaccttttg gaacaacatc
     1561 cacgcccttg agcccggcac caccttgtcc gttcaggacg gccagatctc ggctaggacc
     1621 tatcatcacc tggatccctc catctttggc accagcactt tgtccctgaa cgacgccacg
     1681 gaccagtacc gcaagctttt ccagcgcgcg gttggctggc aaatgcgctc cgatgtggaa
     1741 gtgggggcct gtttaagcgg aggccttgat tcctcggcga tcgtgtgcag tgccacacag
     1801 ctcagcgaga cccctttgca aaccttttcc tcatatttcg ctgaactgcc ccagcttgac
     1861 gagcggcgct ggatagctga agtggcctcg gcttgcggct gtgtgtcaca tctttgctca
     1921 cctgaggttg atgacgctct ttgctggttc gctgaagcca cctggttcaa cgatctgccg
     1981 ctgggatctg gatttgccgc gcaatactcc gttatgcgac tggcgcacga atacggcgtg
     2041 aaagtgctgc tggacggcca gggcagcgac gaactcaccg ccggctacaa acacgcgcaa
     2101 taccgttggc tggcagattt gctgctcgac cagcagcaag gcaggttctg gccggaattg
     2161 aaggcttatt tggcctccaa tcccattccc aaagggctgt ccgggatagc caaaaccctc
     2221 ctcgcgaagg ctttaccgga atcccgtctt tacgctctgg aattccggca cctgcgcttt
     2281 gaacccttca accgggagtt taccagccag gcaaggaact ccttggaaag cggaatcttg
     2341 gcgcgcctcg aagacatcga gggcaccaag ctggccaatt tcctttataa cgaaatctat
     2401 ttcacttccc tgcccaccct gctgcactgg gcagaccgta tgtccatggc cgcctcagtc
     2461 gagagcaggg ttccctttct ggatcacgaa ctggtggaat tcgctttttc tctgccctcg
     2521 tcccacaagg tccatcaggc caccggaaaa cgcatacacc gtcaggccat gcgttccatc
     2581 gtgcccaaag caatcgcgga acgccgtgac aaagcggtgt tcggctcccc ctttcacgct
     2641 ttgtggatgc gcgggggaat gaaatcggct gtggaagagc ttttcagctc cacggaattc
     2701 cgctgcaggg gaatctggga cctgccccgg atctggacta actggcagaa gtataaaaag
     2761 ggcgacaatt cccaagcgga gatgatcttc aacgtcttcg cgctggagaa ttggttccgt
     2821 gaattcggtc cttggattga ttatgaccaa caaacataaa acaaacacat gaggtaatta
     2881 tgcgcaaagc actatattgt atgctgatct gcctggccat gcttggcctc gggtcatgca
     2941 aatggttctt caagagcccc aaagttgaac aaatccatga tatcaaagtc gtctccattg
     3001 atccggacaa gatcaggctg gagatatcgc tcagcgtgct caaccccaac cgctacaaac
     3061 tcaaactgaa cgacatggac ctgattttgc tgaataaaaa ccgggaacag atcggcacag
     3121 cttccctcaa ccgcgctgtg gaaatccccg gccgcaaagc caacgccctc ggttttgaca
     3181 tcacgctgga cacccggccc acggtgaaac tggttaatta ctctgaccaa acagtctttg
     3241 tttacatcgc gggaagagga tccggaaaag tgctgggaac caggcagagc ctggtttttg
     3301 aggaacccta cgaaatagac atccggagcc aactgcaaaa ggtgctgggc accttcaaag
     3361 ccaatggcga cgacattttc aagatcaaac gcagctacct gggcaaagtt ggaatcaccg
     3421 aaacccaaat cctggtggat ttcatcgtga tgaatcccta cggcctcaaa ttcaccctgg
     3481 cagatttccc cgccaccatc accatcggca atgcggagtc cggctccggc aaccttgaca
     3541 aagcccttaa cttcgatgaa aaggtctatt cccgcgaagg caccatgatt ttcaaggtta
     3601 acaacctgaa ggccatctac aacatcgcca agggcgctat caacggcgaa gtgagctacc
     3661 gggtggacgg agaagtgaaa atcaacgcct acggggctga aatgaaacag tcctattcct
     3721 ataaggacct gatcactata agcatcgcag acgaaatcat caagctgctg gttcaataag
     3781 ccatagaatt ttataacggc gaacccggga tgccggcatt agtggacaac tgtgcctgtt
     3841 atcttgttga ctggcattta gttatttaat atagagctcc aagccaagtt cggtctgctc
     3901 ctcagtttca tgctgaaggc agacccttag gaatcgtcca cgtttatgcc attgtcccga
     3961 agttttttga gcagatcagc gcggatggaa gccagtttct ttttctgttc cacccagtcc
     4021 gagtagagat aaagtagggt aacttcgacc acctcattga cccttaaact gatgcgaaac
     4081 gtcaacttgc tgtaatccac gggaccaaag cccataccct cgtcacggat tctggacact
     4141 ctgcttactt gctccagttc aaaactacgg ccatctggaa gcgagtaaag catataatct
     4201 cctatccctg actcttcttt tggacatttt cccggatgtg ccgtttattg gcaagtcttt
     4261 ttaatagacc cgaggtgtga aattagcgca gttgttgatt tttttacaca atctcccggg
     4321 cctcatcgtt attggggaga tattgcaact atggttgaat tgtgtgctgc aagaagttag
     4381 cttttcccag aaaaggcttg aaaccacctt gtttgaaaaa ggttaataat tcctaattta
     4441 ccatcagcca cgccctgagc ggtatcgtca atccctctcc cccacctccc gcaaaatact
     4501 cgcatcaaat gcgggcatcg tgcgggaggc gaatccggaa cacgggaaag ggccttaacg
     4561 gccaatcaac atcgtttcat acaaggcatt cgctggtatc gagttaagaa agaatatcga
     4621 cggcagcaaa gaacaatcac accaaggtat tgtgccgcaa aatctaaccc gcatcatatt
     4681 gaaaaaaaac cataatttac ttgaccgatt ccggtatttg gcagtttggt gagtttaagg
     4741 agaacaggat gaaaagaaaa cttctcttaa tcttgagctt gctggcctgc ctggccctgc
     4801 tctgctcttg cgcgcccgga attggtggca gatcagccga gaaccgggcc ggttttttca
     4861 tgggtatctg gcatggctgg atcgcgccat tcagccttat ctggcattgg tttaaccgtg
     4921 atgtgcgcct ttacgaagtc tttaatacag ggtgggcata cgattttggc ttctacatgg
     4981 gtgtggtggg cggttttggc agccttgccc tcagccgtaa aagacgccgc gatgattgat
     5041 atgaccggta aacaggcatg gccgcatatc tgaccctggt cctcaccgcg gcgatctggg
     5101 gtctggcctt cgcggcccag cgcaagggga tggagagttt ggaccccttt accttcaacg
     5161 ctttgcgctt cgctttgggg gcggcttgca tagccctgac aggcttgatt tccaaagcaa
     5221 aaaaacccaa cccggagatt ccggtcacag accacacccc ttctaacggc aaaagctgga
     5281 aagggccgct gcttttggga ttgatgctgt tcctcgcctc cagctttcag cagtttggca
     5341 tgctttggac cggagccggc agcgcgggct tcatcaccgg tctttatgtg gtgtttgttc
     5401 ccttggtcgg tttgg
//
LOCUS       DAOOWR010000090         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_15321_length_5385_cov_3.349906, whole genome shotgun sequence.
ACCESSION   DAOOWR010000090 DAOOWR010000000
VERSION     DAOOWR010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15321_length_5385_cov_3.349906"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLB85_03260"
     CDS             <1..257
                     /locus_tag="PLB85_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU72377.1"
                     /translation="SVSHLDVSLQVYLQRSLMVLGPGELLITYVKSVVSAWLIVIIAA
                     HQGFRVRGGAEGVGNSTTRSVVASILAIIFADAVFSVVYL"
     gene            254..997
                     /locus_tag="PLB85_03265"
     CDS             254..997
                     /locus_tag="PLB85_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU72378.1"
                     /translation="MNAPLLSVRGLQARYGESLILKDIDLDIMPNEVAVILGSSGSGK
                     TTLLKNILGLEWPASGSVKYRGENIMEMDEARYREVLIRFGVLFQSGALLNSISVFDN
                     IAIPLEQHSRLCPKVIERIIQAKLSLVHLDGAIYQLPSELSGGMRKRAALARAIALDP
                     EILFCDEPTSGLDPLTARALDELLLDLKHKLNMTIVIVTHEIASIKRLADRIFFIDKG
                     SLIFSGTLKEALSAGIGPIDRFFEAGRFD"
     gene            990..1484
                     /locus_tag="PLB85_03270"
     CDS             990..1484
                     /locus_tag="PLB85_03270"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU72379.1"
                     /translation="MTEPRVYLIAAIDRNNLIGRENRMPWNIREDLRRFRAITLGHTI
                     VMGKNTWLSLGRTLDGRVNVILSRDRELAVPGAIVCHSVSECLQHCAGDECFVIGGAQ
                     IFKLFLPLASKLFLTRIDAEFEGDTYFPEINPDDWELTSFEAISSETGFNLTFTEYRR
                     CRKP"
     gene            2072..3349
                     /locus_tag="PLB85_03275"
     CDS             2072..3349
                     /locus_tag="PLB85_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU72380.1"
                     /translation="MRRLSSLILILALLVAGCGKAAKSDKVHIRFWHGLSGPLGDVLN
                     EMIVEFNKTHPDIEVIANPISSYNALQQKLSASLQVGKQPDLAQVFESWAAKYQQDEV
                     LVSIDSLLANDPDFTKEELADFYPVFLQSITYQGKKWSFPFNKSVRAYFYNKDAFYRA
                     GLGPNRFPATWAEFEDYCRKLTSPKTKDRGETYGANFTVNEWQFVNLIHQAGGTIMDE
                     NDKPVLNSPAGLEALNYTLNLVNKEKTAKASKEMDGQNAFLAGTVAMFEGSSVSITWM
                     RQQPINFNIGYAPLPTYKTNKSAISGANIVIFKSGDPKKEKAAWEFIKWFTDTEQTAK
                     WSARTCYMPLRKSAMKSKTIVDFLNTYPQFRGIYAQLDNAVFEPQHPAWFVGRDELKK
                     ALEKAKEGMLSPQAALDEAAAKLQEAIDREAGF"
     gene            3460..3547
                     /locus_tag="PLB85_03280"
     tRNA            3460..3547
                     /locus_tag="PLB85_03280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3494..3496,aa:Leu,seq:caa)
     gene            3724..4752
                     /locus_tag="PLB85_03285"
     CDS             3724..4752
                     /locus_tag="PLB85_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOU72381.1"
                     /translation="MSKYNMLILAVLITILSLPLSLGAISQASMLSLTFEPGGRANGM
                     GRAYSAVADDAYAPWWNPGATAFNRKTQLAGSHIPWLAGSGFDDMYYEYLGYNQYFEG
                     IGNINAHLTLLDMGTQTQTDENNNVIGEFHSFEAAGNVGYSYDVIPQQLGIGTNFKFV
                     YSYLGPGQPTEPDGKAFGFAFDLGAKYQDFLTRGTNLSLVIQNIGPNVTYIDQEQSDP
                     LPMTVRLGAAYEILNAPMNKLLVSAEASKVLANEDPLLTRLFNTGWQHMDETIYGVGA
                     EYTYLDLISLRGGYFLDTAGSVVGPSFGAGIQYTFSKKYKLTADFSMVPGGELVDFNK
                     VFSLGFEF"
     gene            4819..>5385
                     /locus_tag="PLB85_03290"
     CDS             4819..>5385
                     /locus_tag="PLB85_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72382.1"
                     /translation="MRIRFLLLLLAITSLLGAEKLTLQKHFSNRPPRRHPHTQLLNKQ
                     PASATKSRDANFERLLVILVDFQAETTDDPNTTGNGKFLLEPDPNYLYSVGSPPHDRA
                     YFQQNLEALRYYYLAASAGSYNLQYEVWPQTGAYTLPKNMGYYNPPGADNELFVSRME
                     EYFKTAFELADSLSPELDFSAFGHYMIIH"
BASE COUNT         1280 a         1551 c         1358 g         1196 t
ORIGIN      
        1 tctccgtttc ccatctggat gtcagcctgc aggtttatct gcagcgttcc ctgatggtgc
       61 tgggaccggg cgaactactg atcacctatg ttaaaagcgt ggtttctgcc tggctaatag
      121 tcatcatcgc cgcccatcag ggatttcggg tgcggggcgg cgccgaaggg gtgggcaatt
      181 ccaccacgcg ttccgtcgtg gcctccatcc tagctatcat atttgccgac gcggtcttca
      241 gcgtggtcta tctatgaacg cacccctgct cagcgtccgc gggctgcagg cacgctacgg
      301 cgaaagcctg atcctgaaag acattgacct ggacatcatg ccaaacgagg tggctgtcat
      361 cctcggcagc agcggcagcg gcaagacaac cctgttgaaa aacatcctgg gcttggaatg
      421 gcccgcctca ggcagtgtga aataccgcgg cgaaaacatc atggaaatgg acgaggcgcg
      481 ttaccgcgaa gtcttgatac gcttcggcgt gctcttccag agcggagcgc tgctcaattc
      541 aatatcggtg ttcgacaaca tcgccattcc cctggaacag cacagccgcc tctgccccaa
      601 agttatagaa cgaatcatcc aggctaagtt gagcctggtg catctggatg gagcaatata
      661 tcagcttccc tccgaacttt caggcggaat gaggaaacgg gcggctctgg ccagggcgat
      721 tgcccttgat cccgaaatcc ttttctgcga cgaacccacc tccggcctcg acccgctgac
      781 ggccagggct ttggacgaac tgttgctcga cctgaaacac aaactcaaca tgaccatcgt
      841 gatcgttacc cacgaaatcg cttccatcaa gcgtttggcc gaccgcatct tctttataga
      901 caagggttct ctcatcttca gcggcaccct caaggaggct ctcagcgccg gaatcggccc
      961 catcgacagg ttcttcgaag cggggcgttt tgactgagcc acgcgtctat ctgatcgctg
     1021 cgattgaccg caacaacctg atcggaaggg aaaaccgcat gccttggaac attcgtgagg
     1081 acctgcgccg ctttcgcgcc ataaccctcg gccacaccat tgtgatgggc aaaaacacct
     1141 ggctgtcgct gggacgcacc ctcgacgggc gggtgaacgt catcctgagc cgcgacaggg
     1201 aactggcggt gccaggagcc atagtctgcc acagcgtttc ggaatgtctg cagcactgcg
     1261 ctggggatga atgcttcgtt attggcggcg ctcaaatctt caagctattc ctgcccctgg
     1321 cctccaaact tttcctcacc cgcatcgacg cggaattcga gggcgacacc tatttcccgg
     1381 aaattaatcc cgacgactgg gaactaacat ctttcgaggc tatcagttcc gaaaccggat
     1441 tcaacctgac cttcacggaa tatcgccgtt gccggaaacc ttgagggttc cggtttttta
     1501 ggctctcttt caacccggtg gcaaacctgg cgtacagcga acatagtgag catggacgac
     1561 aggacatttt ggtgaattta ttcattgctg gcgtcgatgc tcgttcctcg ctcgacacca
     1621 gcttttggcc gatgttgact ggccgttaag gctttgtcca ttatcccaaa agcgcctccc
     1681 gcataatacc cgcatttgat acggggattt ggcgaggggc atgagagggg gatggtcgca
     1741 ggcgttaacg gcctggctgg caggacactt cagggcgcaa acttttctgc catcttccag
     1801 gctgactttc actattaaaa agggttctct ttcaatctga gtggataaac ccgttgtttg
     1861 agcccgtagg gctacccact cgatttggaa aagagcctta aaaagtgagc ttgattgagc
     1921 ataaccatct cggttcagat gcttttccta aactaattgc aaaggaggct atgttccagc
     1981 cgtggagaaa aagattgact gcaaagcgag ggtgaattat tctgtaatcc atgatgctct
     2041 ggtggcatca ataccgaaaa aggacaagaa aatgcggcgt ttatcaagcc tgatcctgat
     2101 tctggccttg ctagtggccg gatgtggcaa ggcggctaaa tcagacaagg tccatatccg
     2161 cttctggcat ggtttgagcg gccccctggg ggacgttctc aacgaaatga tagtggaatt
     2221 caacaagacc cacccggata ttgaggttat cgccaacccc atcagcagct acaacgccct
     2281 gcagcagaag ctttccgctt ccctgcaggt gggcaagcaa cccgatctgg cccaggtttt
     2341 cgaatcctgg gcggccaaat accagcagga tgaagtgtta gtgagcatcg acagccttct
     2401 ggccaacgat ccagacttca ccaaagaaga gctggcggat ttctatcccg ttttcttgca
     2461 gtccatcacc tatcagggca aaaaatggtc cttccccttc aataaaagcg tgcgggctta
     2521 tttctacaac aaagacgcct tttaccgcgc cgggctgggc cccaaccgct tccccgccac
     2581 ctgggctgaa tttgaggatt attgccgcaa actgacctcg cccaaaacca aagaccgcgg
     2641 cgaaacctac ggcgcgaatt tcaccgtgaa cgaatggcag ttcgtaaacc tcatccatca
     2701 agccggcggc accatcatgg acgaaaacga caaaccggtc ctgaacagcc ctgcagggtt
     2761 ggaagcgctg aactatactc tgaacctggt aaacaaggaa aagaccgcca aggcttccaa
     2821 ggaaatggac ggccagaatg cctttctggc aggcacggtg gccatgtttg aaggttccag
     2881 cgtatccatt acctggatgc gccagcagcc catcaatttc aatatcggct acgctccgct
     2941 gcccacctat aaaaccaata aaagcgccat cagcggcgcc aatatcgtga tcttcaaaag
     3001 cggcgacccc aaaaaggaaa aggccgcctg ggaattcatc aagtggttca ccgatactga
     3061 acaaaccgcc aagtggagcg cccgaacctg ctatatgcct ctgcgcaaga gtgccatgaa
     3121 atccaaaacc atcgtggatt tcctgaacac ctatccccag ttccggggca tttatgccca
     3181 gctggacaat gcggttttcg agccccagca tccggcttgg ttcgtgggca gggacgaact
     3241 gaagaaagcg ctggagaaag ccaaggaagg catgctttct cctcaagccg ccctggatga
     3301 ggcagccgcc aaactgcagg aagccattga ccgcgaggcg ggattctgat gaatcatcct
     3361 tcatccccgc aattgttgat aatttgctac aatcgtcaag cctatccaag atattctgct
     3421 tgacaagcct taggactttg ggaaaaaggt tcgaatcgtg ccgaagtggt gaaacaggta
     3481 gacgcagtgg actcaaaatc caccggactt ttagtccgtg ccggttcgat tccggccttc
     3541 ggcaccacaa aaatatggga gaaaaacgct catttagatt tgctaaaagg ctgcattttt
     3601 gtagaacagc tgcgaaatga ctttttattg gggtattttc tttccctgaa accgataaac
     3661 caatatcaac cgcgacgggt tatttcacgc ccgcgccatt tacagtatct caatggaggt
     3721 ataatgtcca agtataatat gctgatcctg gcagtgctga taaccatttt gagcctgccc
     3781 ttgtccctgg gtgcgatttc ccaggcttcg atgctctcac tcacttttga gccgggcggg
     3841 cgggctaacg gcatgggccg ggcctattcc gccgttgctg acgacgctta cgccccctgg
     3901 tggaacccag gcgccacagc gttcaaccgc aaaacccagc ttgccggttc acacattccc
     3961 tggctggcag gttccggctt cgacgatatg tattatgaat acctgggcta caaccagtat
     4021 ttcgagggca ttggaaacat caacgcccac ctgacgctgc tggacatggg cacgcagacc
     4081 caaaccgacg aaaacaacaa cgttatcggc gaattccata gctttgaagc tgccggaaac
     4141 gttggctaca gctacgatgt gatcccgcag cagttgggca tcggcacgaa cttcaagttc
     4201 gtttacagct atctggggcc cggccagccc accgagccgg acggcaaagc ttttggtttc
     4261 gctttcgacc tcggtgcaaa atatcaggat tttctgacca gaggaaccaa cctctcgctc
     4321 gtgatccaaa acatcgggcc gaacgtgacc tacatcgacc aggagcaatc cgatcccctg
     4381 cccatgaccg tcaggctggg cgccgcttac gagattctca acgcccccat gaacaaactg
     4441 ctggtctcag cggaagccag caaagtcctg gccaacgagg accctctgct caccaggttg
     4501 tttaatactg gctggcagca catggatgaa accatctacg gcgtcggcgc tgagtacaca
     4561 tatctcgacc tgatctccct gcgcggcggc tatttcctgg atacggccgg aagcgtggtg
     4621 ggacccagct ttggcgccgg catccaatac actttcagca aaaaatacaa gctgaccgcg
     4681 gatttcagca tggtccccgg cggcgagctt gtggatttca acaaagtctt ttccctcggc
     4741 ttcgagtttt agggataaag tgattcaagc ctgtctcccc gcgtcttccg acgtggggag
     4801 ctgtggcttt ggctacggat gagaatcagg tttctccttc tcctgctggc gattaccagc
     4861 ctgctcggtg ctgaaaagct cactctccaa aaacactttt caaaccgtcc tccgcgccgg
     4921 catccccaca cccagctgct gaacaagcag cccgcctctg cgacgaaatc ccgggacgca
     4981 aactttgaaa ggttgctggt gatcctggtg gatttccagg ctgaaaccac cgatgacccc
     5041 aatacaaccg gcaacggcaa attcctgctg gaacctgatc ccaactacct ttacagcgtc
     5101 ggcagccccc cacacgaccg ggcctatttc cagcagaatc tggaagcttt acgctattac
     5161 tacctggccg cctcagccgg ttcctacaac ctccaatatg aggtctggcc acaaactggg
     5221 gcttacaccc tgcctaaaaa catgggctat tacaaccctc ccggcgcgga taacgaactt
     5281 ttcgtttccc ggatggaaga gtatttcaaa accgctttcg aactggcgga cagcctttct
     5341 ccggaactgg atttctctgc tttcggacat tacatgatca tccac
//
LOCUS       DAOOWR010000091         5320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_15567_length_5320_cov_3.531434, whole genome shotgun sequence.
ACCESSION   DAOOWR010000091 DAOOWR010000000
VERSION     DAOOWR010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15567_length_5320_cov_3.531434"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..893)
                     /gene="lpxA"
                     /locus_tag="PLB85_03295"
     CDS             complement(120..893)
                     /gene="lpxA"
                     /locus_tag="PLB85_03295"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOU72383.1"
                     /translation="MSLIHPTAIIHPGAVIGEDCEIGPHCQIGPEVVLGCGNKLHSHV
                     ILMGEAHIGDGNQIFSYAVIGTDPQDLKYRGEKTGLSIGNRNVIREFVTINRSNCSSE
                     LTSVGDGNLLMEYVHVGHNCQIGSGCVIANTVQMAGHLVIEDYVSIGGLTAIHQFVHI
                     GTHAFVGGASAVKKDIPPYTRGMGNPYKTSGLNSVGLQRKGFSSESLDSIKEIYRVFY
                     RQGLNVSQALEIVESWPTLTPEQRIFLKFVRSSERGLSN"
     gene            complement(890..2287)
                     /locus_tag="PLB85_03300"
     CDS             complement(890..2287)
                     /locus_tag="PLB85_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOU72384.1"
                     /translation="MAEFKHTIKSEVSYTGVGLHSGEISTIRFKPAGKDEGIVFIRTD
                     LPDKPEIPADIDHVVDISRGTTIGLKGVTVGTIEHVLAAVKGLMIDNIRIEIDGPEVP
                     VGDGSPLVFVKLLKQAGYEEQDSERVYFEFDEAISYSCPEDNVDVVVVPSNELKVTFM
                     IDYKHPHLGTQYTFLPNLRHFERDFAGARTFCFIKEILQLKEQGLIKGGSLDNALVIA
                     EPDMSTAELEHLREVFNYHKPIMVSPEGILESHPLRYSNEFVRHKVVDLIGDMALLGM
                     PIRGHILAARSGHKTNVELVKKLRQIQLKHELKKKYQKDHNKDLVFDINAIMRIIPHR
                     YPFLLVDKIIDLKPGESITGIKNVTINEPFFQGHFPGHPIMPGVLIVEGMAQTGGIIL
                     MRQMDDPAEWVAYFASIDNVKFRKPVLPGDTLRYELKVISLKRSLSKMHGEAYVGDEK
                     VAEGDFLAMLQKRDS"
     gene            complement(2291..>2977)
                     /gene="lpxD"
                     /locus_tag="PLB85_03305"
     CDS             complement(2291..>2977)
                     /gene="lpxD"
                     /locus_tag="PLB85_03305"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOU72385.1"
                     /translation="VLGEDIHIGPGCVIGKGCRIESGAVIESHCVIGAECAIGKNTRL
                     HPRVTLYPFSFIGSECIIHSGAVIGADGFGFVLEDGVQLKIPQVGNVQIGDHVEIGAN
                     STVDCGTLGPTSIGDGTKIDNLVQIGHNCRIGKHCILCAQVGLAGSTVLGDYVYLAGK
                     AGAAGHLSIGDRAMVGAQGGVTHDLAPDGLYWGTPAIDANAYKRILAIQRKLPEIYGH
                     YRKQLKHKED"
     assembly_gap    2978..2987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2988..3327)
                     /locus_tag="PLB85_03310"
     CDS             complement(<2988..3327)
                     /locus_tag="PLB85_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOU72386.1"
                     /translation="MRKFASALSGAEIQAICGGEFRGDDKLQAASVADPAEATADSVV
                     YFEQSRLFEAVRESQAGLVICMPDQANLLPGRNLLLHPRPGFAMMKLVSWWLSHEAEK
                     PAPGIHPSVEA"
     gene            complement(3324..3938)
                     /locus_tag="PLB85_03315"
     CDS             complement(3324..3938)
                     /locus_tag="PLB85_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU72387.1"
                     /translation="MKRTLILLTCLVLVSIVFAADVKLGYVNTDRLLLESNEAAEISR
                     LFNLDKQNWTNQVKKLDDEIKQMERNFEIDKLTKTEAAKRDAQAKIDAKKAEAGRLLE
                     EYFGEGGKAEQRYRELIDPLTLKIHNLIVKTAEDEKYTMIFDVSMGTVLYALPALDLT
                     EQILLELNKDTQPPADGGLLQNEVEFEDPFKTEEPKEEFPDFKP"
     gene            complement(3969..>5320)
                     /gene="gcvPB"
                     /locus_tag="PLB85_03320"
     CDS             complement(3969..>5320)
                     /gene="gcvPB"
                     /locus_tag="PLB85_03320"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU72388.1"
                     /translation="LRETAPRLPQVSELDVMRHYIKLSHRNHFIEKGLYPLGSCTMKY
                     NPKSHEALVRHSCFNNIHPCQPESTLQGSLEILYELQQDLAEISGMHKVTLQPVAGAQ
                     GEFTGIKVIAAYHRAKGNHHKTKIIIPDSAHGTNPATCSLVGYDVVELKSDARGRCDM
                     EHLKSIVDDSIAGFMLTNPNTLGLFEDQIEEIADIIHSVDGLIYMDGANLNALLGIVQ
                     PGKIGFDVMHFNLHKTFATPHGGGGPGSGPVGVVEKLAPYLPVPTIGHGEDGYFFDYG
                     HAETSIGKVHTFHGNFAVLLRAYIYIKMLGAEGLKRVSENAVVNANYLMVLLKDHYHI
                     QHTEHCMHEFVADSLWQKKEFGVTTLDIAKRLLDKGFHAPTVYFPLIVPEAMMIEPTE
                     TESKESLDAFAAAMIEITQECRDNPELLHEAPLTTPVRRVDDVRAVKFLDVAFSPGE"
BASE COUNT         1075 a         1428 c         1474 g         1333 t
ORIGIN      
        1 catactccat ctataatatt actcaataat cttggcatgc tgttttttgt caatgcaaaa
       61 agccggtaat catcctgttc tgtctgctcc cgatgactcc gatgaggaag cttcggatgt
      121 cagttcgaga ggcctctctc tgaagagcgc acgaatttca ggaaaatccg ttgctcggga
      181 gtgagggttg gccaggattc cacgatttcc aacgcttggg agacgttcaa cccctggcgg
      241 taaaagacgc ggtaaatctc tttgatggag tccaggcttt cgctggaaaa gcctttgcgc
      301 tgcaaaccaa cgctgttcaa gccggaggtt ttgtaaggat tgcccatgcc ccgggtgtag
      361 ggcgggatgt cctttttcac tgcggaagcc ccacccacaa aagcgtgggt gcctatatgc
      421 acgaactggt gtatggcggt aagtccgcca atgctaacat aatcttctat caccaaatgc
      481 cccgccatct gcactgtgtt ggctatcacg cacccggagc cgatttggca attatggccc
      541 acatgcacat actccatcag caggttgccg tcaccaacgc tggtgagttc gctgctgcag
      601 ttgctgcggt ttatcgttac gaattcacgg atcacgttgc ggttgccgat gctgaggccg
      661 gtcttttctc cgcggtattt aaggtcttgg ggatcggttc caatcactgc gtaagagaat
      721 atctggttgc cgtcgccgat gtgagcctca cccataagga taacatggga gtgaagcttg
      781 tttccacagc ccaaaaccac ttccggcccg atctggcaat gggggccgat ttcgcagtct
      841 tcaccaatca cggctccggg atggatgatg gcggtgggat ggatcaggct catgaatctc
      901 ttttctgcag catggccaga aaatcgccct cggcaacttt ctcatcgccc acataggcct
      961 cgccgtgcat cttggaaaga ctgcgtttca gcgagatgac tttcagttcg tagcgcaggg
     1021 tgtcacccgg caatacgggt ttacggaact tcacgttgtc gatggaggcg aaataggcca
     1081 cccactcggc aggatcatcc atctggcgca tcaggatgat gccgccggtc tgggccattc
     1141 cctcaacgat caacacgccg ggcatgatgg gatggccggg aaaatgcccc tggaagaaag
     1201 gttcattgat ggttacgttt ttaatgccgg tgatagattc gcccggtttc aggtcgatga
     1261 tcttgtccac cagcaggaaa ggataacggt gggggatgat cctcatgatg gcgttgatat
     1321 caaagacaag gtccttgttg tggtcttttt ggtatttctt tttcaactca tgcttcagtt
     1381 ggatttggcg aagcttcttc accagttcca cattggtttt gtggccgctg cgggcggcga
     1441 ggatgtggcc gcggatgggc atacccaaga gggccatgtc accgatcagg tccacaacct
     1501 tgtggcgcac aaactcgttt gaatacctca gcggatggct ttccagaatg ccttcggggg
     1561 aaaccatgat gggcttgtga tagttaaaga cttcgcgcag atgctcaagt tccgccgtgc
     1621 tcatgtccgg ttccgctatc accagggcgt tgtccagcga accgcctttg atgaggccct
     1681 gttcctttag ctggaggatt tccttgatga agcagaaggt gcgcgctccg gcaaaatccc
     1741 gctcaaagtg cctaaggttc ggcagaaagg tgtactgagt gcccagatgg gggtgcttgt
     1801 agtcgatcat gaaggtcacc ttcaattcgt tcgacggtac cacaaccaca tcgacgttat
     1861 cttcagggca ggaatagctt atcgcctcgt caaattcgaa atagacccgt tccgaatcct
     1921 gctcttcgta gcctgcctgt ttcagcagct tcacgaaaac caggggggaa ccatctccca
     1981 caggcacttc cgggccgtct atctcgatgc ggatgttgtc tatcatgagg ccctttacag
     2041 cggccaaaac atgctctatg gttccaacgg tcacgccttt caatcctatg gttgtccctc
     2101 tggatatatc cacaacgtga tcgatatctg ccgggatttc cggtttgtcc ggcaagtcgg
     2161 tgcgaatgaa gacgatgccc tcatcctttc cggccggctt gaagcggatg gtggaaatct
     2221 ctccggaatg aaggccgacc ccggtgtagc ttacttccga tttaatggtg tgtttaaact
     2281 ctgccattgc tcagtcctct ttatgtttca actgtttgcg gtagtgccca tagatttcgg
     2341 gcaattttct ctggatagcc agaatgcgtt tgtaggcgtt ggcgtcaata gccggcgtgc
     2401 cccaataaag cccgtcgggg gcaaggtcgt gggtaactcc gccttgggcg ccaaccatgg
     2461 cacggtcacc aatgctcaaa tgcccggcgg ccccagcttt gccagccaag taaacgtaat
     2521 cacccaatac cgtgctgcct gcgaggccca cctgcgcgca aaggatgcag tgtttgccga
     2581 tccggcaatt atggccgatt tgcacgaggt tgtcgatttt ggtgccgtca ccgatgctgg
     2641 tgggaccaag ggttccgcag tccacagtgg aattggcacc gatttcgacg tggtcgccga
     2701 tttgcacatt gcccacctgg gggattttta gctggacccc atcctccaga acgaatccaa
     2761 acccatccgc tccgatcacc gctccgctgt ggatgatgca ttcagagccg atgaagctga
     2821 atgggtagag tgtaacccgg ggatggagcc gggtgttttt tccgatggcg cattcggcgc
     2881 cgatcacgca atgcgattct atcaccgctc cgctttcgat ccggcaaccc ttgccgataa
     2941 cgcagcccgg gccgatgtgt atgtcttcgc ccaaaacnnn nnnnnnnggg cctccacgct
     3001 gggatgaatg cccggtgcgg gcttttcagc ctcatgcgaa agccaccagc tcaccagctt
     3061 catcatggcg aatcccggcc tgggatgcag caataggttc cgccccggca gcagatttgc
     3121 ctggtccggc atacagataa ccagccctgc ctgggattcc cttacagctt caaaaagacg
     3181 gctttgttca aagtaaacaa cgctgtctgc tgtggcttcg gcggggtcag ccacgctggc
     3241 agcctgcagt ttgtcatcgc cccggaattc gcctccgcag attgcctgga tttctgctcc
     3301 ggaaagggca gaagcgaatt tcctcatggc ttgaagtcgg ggaactcttc tttcggttct
     3361 tcggtcttaa agggatcctc gaactcaacc tcattttgca gcaaacctcc atcagcgggc
     3421 ggctgggtgt ctttgttcag ttccagcaga atctgttctg tgaggtccag agccggaagg
     3481 gcgtaaagca ccgtgcccat gctaacgtcg aagatcatgg tgtatttctc gtcttcggcg
     3541 gttttcacga tgaggttgtg gattttcagg gtgaggggat cgatcagttc gcggtaacgc
     3601 tgttccgcct ttccgccttc gccgaagtat tcttccagca ggcgtcccgc ctcggctttt
     3661 ttggcgtcga tcttggcttg ggcgtcgcgt ttggcggctt cggtcttggt aagcttgtcg
     3721 atttcaaagt tgcgttccat ctgcttgatt tcatcgtcaa gcttcttgac ctggttggtc
     3781 cagttttgct tgtccagatt gaacaggcgc gagatttcgg ccgcttcgtt gctttccagc
     3841 agaaggcggt ccgtgttcac atagcccagc ttgacatcgg cagcaaaaac gatcgatacc
     3901 agcaccaggc aggtcagcaa gatcagtgtt ctcttcattt tatcttatct ccaaatagtc
     3961 tcgtgggttc attccccggg ggaaaaggcc acgtcgagga atttgacggc gcgcacgtcg
     4021 tcaaccctgc gcaccggtgt ggtgagcggg gcttcatgaa gtaattccgg gttgtcgcga
     4081 cattcctgcg tgatttctat catcgcggcg gcgaaagcgt ccaaggattc cttgctttcg
     4141 gtctccgtgg gttctatcat catggcttcg ggaacgatca gcggaaaata gaccgtgggc
     4201 gcgtggaatc ctttgtccag caacctcttg gcaatatcca gagtggtcac tccaaactcc
     4261 tttttctgcc acagcgaatc cgccacgaac tcgtgcatgc aatgttccgt gtgctggatg
     4321 tggtaatggt ctttcagcag gaccatcaga taattggcat tgaccacagc gttttcggac
     4381 acccgtttca ggccctccgc gcccagcatc ttgatataga tataggcgcg cagcagcact
     4441 gcaaagttgc cgtggaaggt gtgtaccttg ccgatggagg tctctgcatg cccatagtcg
     4501 aagaaatagc catcctcgcc gtggccgatg gttggcacag gcagataggg cgctagtttt
     4561 tccaccacgc caacaggccc gctgcccggt ccgccgcctc cgtggggcgt ggcgaaagtc
     4621 ttgtgcaggt tgaagtgcat cacgtcgaag ccgatcttgc cgggctgcac aatgcccagc
     4681 agcgcattca ggtttgcccc gtccatgtag atgaggccgt ccacgctgtg gatgatgtct
     4741 gcgatttctt cgatctgatc ttcaaaaagg cccagcgtat tgggattggt gagcataaag
     4801 ccggcgatgc tgtcatcaac tatgcttttc aggtgctcca tatcgcagcg cccgcgggca
     4861 tcagatttca gttccaccac atcataaccc accagggaac aggtggcggg gttggttcca
     4921 tgcgctgaat ccgggatgat gattttcgtc ttgtggtgat tgcctttggc ccggtgatag
     4981 gcggcgatca ccttgatacc ggtgaattcg ccctgagccc cagccacagg ctgcagcgtg
     5041 actttgtgca tgccggaaat ctcggccagg tcctgctgaa gctcgtaaag gatttccagc
     5101 gagccttgca gggtggattc aggctggcag gggtggatgt tgttgaaaca ggaatggcgc
     5161 accagggctt cgtgggactt gggattgtat ttcatggtgc agcttcccag agggtagagc
     5221 cctttctcga tgaagtggtt ccggtgcgaa agctttatgt agtggcgcat tacgtccaat
     5281 tcgctcacct ggggcagccg gggagccgtt tcgcgcagat
//
LOCUS       DAOOWR010000092         5218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_15987_length_5218_cov_4.615340, whole genome shotgun sequence.
ACCESSION   DAOOWR010000092 DAOOWR010000000
VERSION     DAOOWR010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15987_length_5218_cov_4.615340"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLB85_03325"
     CDS             <1..419
                     /locus_tag="PLB85_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOU72389.1"
                     /translation="ERLLGSGDMLFLPPGKALTERIHGAFVSDQEIARVCDFLAMQPK
                     PKQDFSITTEEDGETGDFDYDDELFPEAARVVVSANTASVSMLQRHFRIGYARAGRLI
                     DLLERARVIGPHLGSKSRDVLATREDLIQRGILREE"
     gene            496..1113
                     /locus_tag="PLB85_03330"
     CDS             496..1113
                     /locus_tag="PLB85_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU72390.1"
                     /translation="MRKNIFILLALLAAWTAGFALSSSELYTKLQSAYKGISTFQASV
                     QQSNYYPQLKKTVSYSGKIYFTPGRMLMSFTKPDIQVLRIENGRVELYDASSNTLFQS
                     AVQPQFGKMNPVEILQLYWTKSAVTITSQSKTSASVKLVPKQDDLVTSLTATLNPTSG
                     IVSKLGYTDKSGNSVSYSFSGIKLNGSIPASVWKQSYPKDVQIIR"
     gene            1123..2178
                     /locus_tag="PLB85_03335"
     CDS             1123..2178
                     /locus_tag="PLB85_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOU72391.1"
                     /translation="MIALIMAGGSGTRFWPASRDAKPKQFLRVAGAKSMLQLTYERLK
                     PLVPADRTYVVTAASQADLVRQHLPEVPAENIVCEPFGMNTAPCIALGLAQLQQLYLP
                     GESMVVLPADHIIRDVEAFQDSLAKAEKVAQTGALVTFGIVPEYPATGYGYIETGEEL
                     EPGVFKVLRFKEKPDLATATEFLRSGSFFWNSGMFCWTIEAIGVAFTEFLPQALNIAH
                     EVVNLRASGADEEKVAEAYSKMPRVPIDIGIMEPARQRVVIPVSYGWSDVGSWKALAD
                     ISASDEEQNYFRRAGFALDAHDNYVYSKKTVALIGVEGLCLVETDDAILVTSKDRSEE
                     VKKIVEQLRKENKDELL"
     gene            2202..2708
                     /locus_tag="PLB85_03340"
     CDS             2202..2708
                     /locus_tag="PLB85_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU72392.1"
                     /translation="MILSLLPVFAAIDPDVGISSHIGTVELRDNGFWLKADPGNELRL
                     LLAPASLLDSLGLALSTGDTLLVEGWRQDELLLVGKIWTSSADSPIILRDLENGNLAT
                     GGTATYWVDDQTCIGCRLCLSQCPTRAITFSKGKARIDSAKCTECGICVEGNGRFRGC
                     PVSAIKKE"
     gene            2724..3641
                     /locus_tag="PLB85_03345"
     CDS             2724..3641
                     /locus_tag="PLB85_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU72393.1"
                     /translation="MWKIYLRAVGAMLFWAITFVWIKVALVTYRPYEIVFLRLALAAL
                     LLFGVIFIFRKRERVQPKDLLYLMMVAFFEPFLYFIGEANGMQFVSSTLGSLIISTIP
                     LVTAMGAWLFLKESINPLLIVGLLVSFSGVALLSFAEPDLSGTLKGVLLLLVAVFAGM
                     FYGITVRRITLKYRSLTVVAWQNLFGTLYMLPVFLIFDWKHFSTVNHSAHGLLTIAAM
                     SIFASVGAFLLYTGVIRELGVMRSNIFTNLIPVFTVGLAWLILKDTITLRTVLGVLLT
                     VMGLLVSQHQDLRHKRMKTSLEDISSYGM"
     gene            3692..>5218
                     /locus_tag="PLB85_03350"
     CDS             3692..>5218
                     /locus_tag="PLB85_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOU72394.1"
                     /translation="MRLSAKTLFQVALLAVSALLLSAQSVKFSVSPTQLKPGDKGVLR
                     ATLTITDPEKKQSYTPAEGEEGYLYLVETGEYPQLKFGKTSYPAPNHKNSDGIWEYYK
                     SLTLSRSFTVSKTAKPGPITINASMSFNLCNKTSGFCDPPRETEGSVKLEILPATAED
                     QAGQEGEVAEEQPLEVDSATADSAGQSTETPADAAPAQEKAGGGKIWYYMLLAILGGI
                     VLNFTPCVLPILPIRAMSMVNQAQKDISKVLIHTLVYTLGVLISFGAIAAVFVIARAS
                     GVNLTYGFLSQSLLYNLIMMSILFLFGLSLMGVWEMTVPGMNAAAKTTSKKGYGGSFF
                     AGVFAFLMGFSCMGPFMGPALEVAVRLSSPMLVLFFLLIGLGFALPFIIISLFPKALK
                     LVPKPGEWMNIFKELMGFVLMYLTWKYFSNVWGLTKSGPYLMKTGLYLVYLGFAAWLY
                     GRFVRPENKKAVQIILGLAAVAVIAASAATLLPWKEEYRPQDTAAASADGMVPADHPG
                     W"
BASE COUNT         1114 a         1546 c         1386 g         1172 t
ORIGIN      
        1 ccgaacgcct gctgggcagc ggcgacatgc tgttcctgcc acccgggaaa gcgctcacgg
       61 agcgcatcca cggcgctttt gtctccgacc aggaaattgc tcgggtctgc gattttctgg
      121 ccatgcaacc caaacccaaa caggatttca gcattacgac ggaagaggat ggggaaacgg
      181 gcgattttga ctatgacgac gagctgttcc ccgaagccgc gagggtagtg gtgagcgcta
      241 acacagcctc cgtttccatg ttgcagcggc atttccgcat cggctatgcc cgggcgggcc
      301 ggctaatcga ccttctggag cgggcgcggg tgattgggcc ccatctgggc agcaaatccc
      361 gcgatgtgct tgccactcgg gaagacctca tccagcgcgg catcctgcgg gaagagtaat
      421 aatgcgcagg aagcaggacg caagggagta ttgattgcaa aggtttattc cgtcaacaca
      481 taccaaggac acaagatgag aaagaacata ttcattctgc tggcgctgct ggcagcctgg
      541 acggccggct ttgccctgag cagttccgag ctttacacga agctgcaatc cgcttacaag
      601 ggcatttcca cctttcaggc aagcgtgcaa cagagcaact actaccccca gttgaaaaag
      661 accgtcagct acagcggcaa aatctatttt accccggggc gcatgctgat gagcttcacc
      721 aagcctgaca tccaggtgct tagaatcgag aacggacggg tggaactcta tgacgcctct
      781 tccaacactc tgttccaaag cgcagtgcag cctcaatttg gcaaaatgaa cccggtggag
      841 atcctgcaac tctactggac caaatctgcc gtgacgataa cctcgcagtc caaaacctcg
      901 gccagcgtta aactggtgcc caaacaggac gacctggtca cctcgctcac tgccacgctc
      961 aaccccacca gcgggattgt gagcaaactc ggctacacag acaagagcgg aaacagcgtc
     1021 agctacagct tttccggcat caagctcaat ggctccatcc cggcttccgt gtggaagcag
     1081 agctacccca aagatgtgca gatcatccgc tgagaaggta tcatgatcgc gttgataatg
     1141 gccggaggtt ccggcacccg tttctggcca gccagccggg acgccaaacc caagcagttt
     1201 ttgcgcgttg ccggcgccaa atccatgctg caacttactt atgagcgtct gaaaccgctg
     1261 gtcccggcgg accgcaccta tgtggtgacc gctgcttccc aggccgacct agtgcgccag
     1321 catctgccgg aggttccggc tgagaatatc gtctgcgagc ctttcggaat gaacaccgcc
     1381 ccctgcatcg ctctaggcct ggctcagctt caacagcttt atcttcccgg cgagagtatg
     1441 gtagtgctgc ccgcggacca tataattcgc gatgttgagg catttcagga tagcctggcg
     1501 aaagcggaaa aggttgccca gaccggcgct ttggtcactt ttggcattgt gccggaatat
     1561 ccagccacag gatacggcta catagaaacc ggcgaagagt tggagccggg cgttttcaag
     1621 gtgttgcgct tcaaggaaaa accggatctg gccaccgcca cagaattcct tcgcagcggg
     1681 agtttcttct ggaacagcgg catgttttgc tggaccattg aagccatcgg agtcgctttc
     1741 acagagtttt tgccccaggc gctgaacatc gcccacgaag tggtgaattt acgcgcttcc
     1801 ggggcagatg aagaaaaggt ggcagaagcc tattccaaaa tgccccgcgt gcccatcgac
     1861 atcggcatca tggaaccagc ccgccaaagg gtggtgatcc ccgtcagcta cggctggagc
     1921 gatgttggct cctggaaagc gctggcagat atctcagctt ccgatgagga acagaactat
     1981 ttccgcaggg ctggtttcgc tctggatgcc cacgacaact atgtttacag caaaaaaact
     2041 gtagccctga tcggcgtgga ggggctctgc ctagtggaaa ccgatgatgc catcctggtg
     2101 acctccaaag acagaagcga agaagtgaaa aagatcgtgg aacaactgcg gaaagagaac
     2161 aaggacgagc tcttgtgaat aagtgccttt tgaccatctt gatgatcttg agcctattac
     2221 cggttttcgc cgcgatcgat cccgacgttg gaatctcctc ccacattggc actgtcgagc
     2281 tgcgtgacaa cgggttctgg ctcaaagccg atcccggcaa tgaactgcgt ttgctgctgg
     2341 ctccggctag tttgctggat tccctgggcc tggccctgtc aaccggcgac accctgttgg
     2401 tggaaggctg gcgtcaggac gaactgcttt tggtgggcaa gatttggact tcctctgccg
     2461 acagccccat catcctgagg gatttggaaa acggaaacct cgccaccggc ggcaccgcca
     2521 cttactgggt ggacgatcaa acctgcatag gttgcaggct ctgcctgtcc caatgcccca
     2581 ctagggcgat cactttcagc aaaggcaagg cccgcataga ttcagccaaa tgcaccgaat
     2641 gcggtatctg cgttgaggga aacggccgct tcagaggctg ccccgtgagc gccatcaaaa
     2701 aggaataggg ttggatgctg aggatgtgga agatttacct tagggctgtc ggagcgatgc
     2761 tcttctgggc catcaccttt gtttggatca aggtggcttt ggtcacttac cggccttacg
     2821 aaatcgtgtt cctgcgcctg gccctggctg ctcttctttt attcggagtg atcttcatct
     2881 tccgcaaaag ggagcgggtt cagcccaaag acctgctcta cctgatgatg gtggcctttt
     2941 ttgagccttt tctctatttt atcggcgaag ccaacggaat gcagttcgtt tcctccaccc
     3001 tcggttcgct gatcatctcc accatccctc tggtgacggc tatgggcgct tggctgttcc
     3061 tgaaggaaag catcaatccc ctgctgatcg tgggactgtt ggtttccttt tccggcgtgg
     3121 ccctgctcag tttcgcggag cccgaccttt ccggcaccct gaaaggagtt ctgctgctgt
     3181 tggtggcggt tttcgccggg atgttctacg gcatcaccgt gcgacggatc acccttaaat
     3241 accgttccct caccgttgtg gcctggcaaa acctcttcgg cacgctctac atgctgccgg
     3301 tattcctgat cttcgattgg aagcactttt ccaccgtgaa ccactccgcc catggtttgc
     3361 tgaccatagc cgccatgtcc attttcgcct ccgtgggcgc gttcctcctc tataccgggg
     3421 tgatccgcga actgggtgtg atgcgttcca acatcttcac caaccttatc ccggtcttta
     3481 cggtcggcct ggcctggctt atcttaaaag ataccattac cttgcgcact gtgctggggg
     3541 ttctgctcac cgtcatgggt ctgcttgttt cccaacacca agatttgcgc cataagcgga
     3601 tgaaaacatc ccttgaagat atttcgtcct atggaatgta aattgcactc aatcattaac
     3661 attaaaatgc cttatccgtg gaggattact gatgcgcctt tctgccaaga cgctcttcca
     3721 ggtggcgctg cttgccgttt ctgccctgct gcttagcgcg cagagcgtga aattcagcgt
     3781 cagccccacc caactgaaac ccggtgacaa aggtgtcctc cgggccacgc tcaccatcac
     3841 cgatcctgaa aagaaacagt catacacacc ggccgaaggc gaggagggtt atctctacct
     3901 ggtcgaaacc ggcgagtatc cccaactgaa attcggtaaa acctcttatc ccgctcccaa
     3961 ccacaagaat agcgacggca tctgggaata ctacaaatcc ctcactctca gccgctcctt
     4021 caccgtcagc aagaccgcca aaccgggacc catcacaatc aatgccagca tgtccttcaa
     4081 cctctgcaac aagaccagcg gtttttgcga tccgccccgg gaaactgaag gcagcgttaa
     4141 gctggagatt ctgcccgcta cggcagagga ccaggccggg caggaagggg aagtcgctga
     4201 agaacagccg cttgaggtag attccgccac agccgattcc gccggtcaaa gcacagaaac
     4261 ccccgccgat gccgcccctg cccaggagaa agcgggcgga ggcaagatct ggtattacat
     4321 gctgctggcc attctgggcg gaatcgtgct caacttcaca ccctgcgtgc tgcccatcct
     4381 gcccatccgg gcgatgagca tggtgaacca agcccagaaa gacatctcca aagtgctgat
     4441 ccacactctg gtttacaccc ttggcgtgct gatttccttt ggcgccatcg ccgctgtttt
     4501 cgtcattgcc cgtgcctctg gggtgaacct cacctacggt ttcctcagcc agagcctgct
     4561 ttacaacctc atcatgatga gcatcctctt cctctttggc ctttcgctga tgggggtttg
     4621 ggaaatgacc gtgccgggga tgaacgccgc tgccaaaacc acttccaaaa agggttacgg
     4681 cggctccttc ttcgccggcg tcttcgcttt tctgatgggc ttttcctgca tggggccctt
     4741 tatgggaccg gcgcttgaag tggctgtgcg cctgtcctcg ccgatgctgg tgttattctt
     4801 cctgctcatc ggcttgggtt tcgccctgcc cttcatcatc atcagccttt ttcccaaggc
     4861 tctcaagctc gtgcccaagc cgggcgaatg gatgaacata ttcaaagagc tgatgggctt
     4921 tgtgctgatg tatctcacct ggaaatactt ctccaacgtc tggggcctca ccaaaagtgg
     4981 cccctacctg atgaagactg gcctttacct ggtttacctg ggcttcgcag cctggctcta
     5041 cggccgcttt gtgcgccccg aaaacaaaaa ggccgtccag atcatcctgg ggctcgccgc
     5101 cgttgctgtg atcgcagcct ctgccgccac cctgctgccc tggaaagagg aataccgtcc
     5161 ccaggatacc gccgctgcca gtgccgacgg catggtgccc gccgaccatc ccggctgg
//
LOCUS       DAOOWR010000093         5206 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16030_length_5206_cov_2.909338, whole genome shotgun sequence.
ACCESSION   DAOOWR010000093 DAOOWR010000000
VERSION     DAOOWR010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16030_length_5206_cov_2.909338"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLB85_03355"
     CDS             complement(<1..929)
                     /locus_tag="PLB85_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOU72395.1"
                     /translation="MNKIGLTDTDIFALVDCNNFYVSCERVFDPSLEGKPVAVLSNND
                     GCLVSRSNEVKALKIPMGAPGFKHEALIRKHGGTLLSSNYALYGDMSRRVMDVLGSFT
                     PELEIYSIDEAFLGLNGFRIRDLEAYGHKIRDTVRKWTGIPVSVGISRSKTLAKVANH
                     YAKRYSGYRGCLVMLDDERIQKALERLPVAKIWGIGRQYDKFLRQNKIENAWQLRNAP
                     EKFIDHYLTSVGHKTVLELQGFSCIDLDDAPSPKQSIVVSRSFGKQVADLAELEEAVS
                     TYITRGAEKLRAQHSVAGHLMVFLNTNRFKEGP"
     gene            1084..2133
                     /locus_tag="PLB85_03360"
     CDS             1084..2133
                     /locus_tag="PLB85_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72396.1"
                     /translation="MSTELRLWIIFHRRIDRNRILKLDSYWGKKDYATEVKFFYREVT
                     MKRVLLSLALVVLIASGYAYWTDIFANYYIPPETFIPVAARIYRNGVYTGYTTPHRFE
                     PNTVYHGTYTVELEQYGPWGPTSAYVAPTAENGVVIFSSSYGPTVPIELSSFTAAISA
                     NNCINLTWVTHSETGVRGYYVLRNNTNELTMATTISGFIPATNTSQTVSYFYADEDLE
                     DEGTYYYWLLNSDLDGQDYYYGPSSIQYSLPGDDIPEPPQTTSLGPIYPNPFNPNAVI
                     PINLKNDSTVSLKIYNNRGQEVRRFDLGNLQAGNHQISWDGRDGSGQPLASGVYQIRM
                     QTGKDNYQVKAVLMK"
     gene            complement(2762..3955)
                     /gene="ftsZ"
                     /locus_tag="PLB85_03365"
     CDS             complement(2762..3955)
                     /gene="ftsZ"
                     /locus_tag="PLB85_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOU72397.1"
                     /translation="MIEFDEKPTQIGTNIKIVGVGGAGGNAVNTMIDNELSGVEFIAA
                     NTDISDLVKSKAKMKLQLGKKLTRGLGTGANPDLGARAAEESKDDIRSHIEGADMIFI
                     AAGMGGGTGTGAAPIVAKIARDLGILTLGIVTMPFPFEGQKRHSNAEEGIRNMAEFVD
                     TLIVIPNSKLCEIYADLTLLEAFQRADNVLFESAKAVSDIINVNGLMNRDFADVRTVM
                     QNMGYALMGSGVAEGENRAVNAAKAAINNPLLADISLQGCQALLINVTAGSDIKMSEF
                     EDVSSVIVNETGTNANIFMGLVIDPDLVGKVSVTIIATGLQSTAQIVDFPPSQPTPQP
                     RQAPQQKPQTPKPGKSEEDIDDIFRILNINQPSSQEADPDPHGEETRQSSPRTDLPSF
                     LKALD"
     gene            complement(3967..>5206)
                     /gene="ftsA"
                     /locus_tag="PLB85_03370"
     CDS             complement(3967..>5206)
                     /gene="ftsA"
                     /locus_tag="PLB85_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU72398.1"
                     /translation="ANIRCILAQAKAKGQLDILGIAATPSTGLEKGIVKDIQAVSEGI
                     KKALKEVQTAAGTKPANIFTNVTGDHIRTQIGDGRISIPTSSPNEPGEIVQEHMDQVI
                     ADARNSVKIQKGFERSQILHGIPQSFVIDGHNDIRNPLRMTGFHLITKVLTIFGDVTQ
                     LRNLAKSIELADYDISPDNFVLNHIAIANAVLSEDERRLGTILIDIGGGTCDISLYNR
                     GILDKILVIPMAGSSITEDLAIGLKTTLSNAEYIKTNYGVALASSVDPETEIVVEGIS
                     GRDSQVKTQHLVSHVVQHRVEEMLSICYDRLKNSYMPELVTAGIVLTGGTAKLQMIDA
                     VVKNSFNLPVKIAQPDLSNLNGDTEMLKDPAFATCVGLLRHAAAQEPESKAPALRLGT
                     FKAGKTLEKIKQIFKDFTST"
BASE COUNT         1099 a         1308 c         1500 g         1299 t
ORIGIN      
        1 tgggggcctt ccttgaagcg gttggtgttc agaaacacca tcagatggcc cgccacgctg
       61 tgttgggcac ggagtttttc cgcgccgcgt gtgatgtagg tggaaacggc ttcctccagt
      121 tcggccaggt ctgccacctg cttgccaaag gaacgcgaga ccacgatgct ctgtttgggt
      181 gagggcgcgt cgtccaggtc gatgcaggaa aagccctgca gctccagcac ggttttatgg
      241 cccacgctgg tcaaatagtg gtcgatgaac ttctctgggg cgttgcggag ctgccaggcg
      301 ttctcgatct tgttctggcg caaaaacttg tcatattgcc tgccgatgcc ccagattttc
      361 gccacgggaa gccgctccag cgctttttgg atcctctcgt cgtccaacat caccaggcag
      421 ccccggtatc ctgaatagcg cttggcgtaa tgatttgcca ccttggccag ggtcttgctg
      481 cgcgaaattc ccaccgaaac agggatgcct gtccatttgc gcacggtatc acgaatcttg
      541 tgtccgtaag cctccaaatc gcggatgcga aagccgttca ggcccagaaa ggcctcgtcg
      601 atggaataga tttccagttc cggtgtaaaa ctgcccagaa cgtccatcac ccggcggctc
      661 atgtcgccgt atagggcgta gtttgaggaa agcagcgtgc caccgtgttt gcggatcagc
      721 gcttcgtgct tgaagccggg cgcgcccatg ggtattttga gggccttgac ctcgttagag
      781 cgggagacga gacagccgtc gttattggac agcacggcca ccggtttgcc ctccagggag
      841 gggtcaaaca cgcgttcgca ggacacataa aagttgttgc aatccaccag cgcaaatatg
      901 tccgtgtcag tcagtccgat cttattcatc tcatttctcc caatgccaaa atatccacag
      961 gccttgagct gtcaatctgt taataccccc agggtcaatg gcaggaagta acaacataga
     1021 aaaactttga atccatgtaa agggacatta agggaggcat atttcaactc caccaaagaa
     1081 ttgatgagca ccgagttaag gctgtggatt attttccatc gacgtattga cagaaataga
     1141 atattaaaac ttgacagcta ttggggaaaa aaagattatg caactgaagt taagttcttc
     1201 tatcgagagg ttaccatgaa aagagtactg ctttctctgg cccttgtggt cttgatcgca
     1261 tcaggctatg cttattggac tgatattttc gccaactatt acattccacc cgagaccttc
     1321 attccggttg cggcacggat ttaccgaaat ggtgtttata ccgggtatac aacgcctcac
     1381 cgttttgaac ctaatactgt ttaccatgga acttatactg tggagttaga gcaatatggc
     1441 ccctggggac ccacatcggc ttacgttgcg ccaactgccg aaaatggggt tgtgatattc
     1501 agctcctcct atggccctac cgtgcctatc gaactgtcga gtttcaccgc tgcgatctct
     1561 gcgaacaatt gcattaacct cacttgggtc acacattcag agactggagt tcggggttat
     1621 tatgtcctgc gaaacaatac caatgaactc accatggcca ccaccataag cggatttatt
     1681 cctgccacaa atacctccca gacagtgagt tatttctatg ccgacgagga tctggaggat
     1741 gagggcacat attattattg gttgctaaac agtgatcttg acggccagga ttattactac
     1801 ggaccaagct cgatccagta ttcgctgccc ggtgatgata tacctgagcc gcctcagaca
     1861 acaagtctcg gacctattta tcccaacccc ttcaatccca acgcggtcat ccccatcaat
     1921 ctgaaaaatg acagtacagt aagcctgaaa atctacaaca accgcggtca ggaagtgaga
     1981 cgctttgatc tgggcaatct gcaagctgga aatcaccaga tcagctggga cggccgcgac
     2041 ggcagcggtc agcctctggc cagcggggtt tatcaaattc ggatgcagac gggaaaagac
     2101 aactatcagg tgaaagccgt cttaatgaaa tagagcctaa taggtaaacg ctacttttta
     2161 caagcccggg cgacgtgctc cgggtttttt gtatctataa catctgagcg cgatcccatc
     2221 cctatgctga atgatgttaa ctttggcact ctttcaagcc gagtgggtaa tccagttaat
     2281 caagcccgta gggcgacata acgatagcga gggagcgcaa gcccctcgta cagtggcaat
     2341 aaccagatgg aaagcccctt gcgggtgaca gaaatcaatc tccgcggttc tgtcgccctc
     2401 cgggctaccc actcggtttg aaagagagcc atttgaaaga actccatgac tgtttagcca
     2461 ccgcaatacc ctaccagtca ggccctgagc gcttgatacc atactcctcc cacgtctccc
     2521 gcatgatact cgcaccgaat gcggggatta tgcgggaggc gcgactggag catgaaggaa
     2581 ggccttaacg gccaaatggc attgcttctc ccgtttgcgg ggattccgga gttgtaatga
     2641 tggcgtttct acctgctcca tttgtatatc ccaaaagaaa accgtcggca agggggagtg
     2701 ccgacggttt gttcggacag ggctgtccgt tgagagacct aagcaggaag gatggaaaag
     2761 ttcaatccag ggctttcaga aaggaaggca gatctgtgcg gggacttgac tgcctggttt
     2821 cctccccgtg tggatcggga tcggcttcct ggctggaagg ctggtttata ttcaagatac
     2881 ggaagatgtc atcaatgtct tcttcggatt tgcctggctt gggagtttga ggcttctgct
     2941 gcggagcctg tctgggctgc ggggtgggtt gggacggagg gaaatcaact atctgggcgg
     3001 tgctttgcag tccggtggcg atgatggtga cggaaacctt gcccacaagg tcgggatcaa
     3061 tgaccaagcc cataaagata ttggcgttgg tgccggtttc gttcacgatc acgctggaaa
     3121 cgtcctcaaa ctcgctcatt ttgatgtccg atccggcggt gacgttgatc aacagggcct
     3181 ggcagccttg caggctgatg tcggcgagga ggggattgtt gatggccgct ttggcggcgt
     3241 tcacggcgcg gttttcgccc tcggccacgc cggagcccat cagggcgtaa cccatgttct
     3301 gcattacggt gcgcacgtcg gcgaaatcac ggttcatcag gccattgacg ttgatgatgt
     3361 cggaaacggc tttggcggat tcaaaaagaa cgttgtcggc gcgctggaaa gcctccagta
     3421 gggtgaggtc ggcgtaaatc tcgcaaagct tggaattggg aatcacgatg agggtgtcta
     3481 caaactccgc catgttgcgg atgccttctt cggcgttgct gtggcgtttt tgcccttcaa
     3541 aagggaaggg catggtcacg atgccgaggg tgaggatgcc aaggtcgcgg gcgatcttgg
     3601 caactattgg agctgctccg gtgcccgtcc cgcctcccat tccggcggcg atgaagatca
     3661 tgtccgctcc ttcaatgtgg gaacggatat cgtctttgga ttcctccgcg gcgcgtgcgc
     3721 cgaggtctgg attggcgccg gttccaagcc cacgggtgag ttttttgccc agttggagct
     3781 tcatcttcgc cttgcttttc acgaggtcgc tgatgtcggt gttcgcggcg atgaattcca
     3841 cgccggacag ttcattgtca atcatggtgt tcacagcgtt gccgccggct ccgcctactc
     3901 caacgatctt gatgttggtg ccgatttgag tgggtttttc atcaaattcg atcatgtttt
     3961 ccccctttag gtacttgtaa agtctttgaa tatttgtttg attttttcca gggtctttcc
     4021 cgctttgaaa gtgcccaggc gcagcgcggg agctttgctt tccggttcct gggcggcagc
     4081 gtgacggagc aatccgacac aggtggcgaa ggccgggtcc ttcagcattt cggtatcgcc
     4141 gttgaggttg cttaggtccg gttgggcgat tttcacaggc aggttgaatg agtttttgac
     4201 cacggcgtct atcatctgga gtttggctgt gccgcctgtg agcacgatgc ccgcggtcac
     4261 gagttcaggc atgtaagagt ttttgaggcg gtcatagcag atcgagagca tttcctccac
     4321 acggtgctgc accacgtggc tgacaagatg ctgcgttttc acctggctgt cgcggccgct
     4381 gatgccctcc acgacgatct cggtctcggg atcgacgctg ctggcgaggg ctacgccata
     4441 gttggtcttg atgtattcgg cgttggaaag tgtggtcttg agtccgatgg cgaggtcctc
     4501 ggtgatggag gagccggcca ttgggatcac gagtatcttg tccaggatac cgcggttgta
     4561 aaggctgatg tcgcaagtgc cgccgccaat gtcgatgagg atggtgccga ggcggcgttc
     4621 gtcctcgctg agcacagcat tggcgatggc gatgtggttt agcacaaagt tatcagggct
     4681 gatgtcgtaa tccgccagtt caatgctttt ggccaggttg cggagttggg tgacatcgcc
     4741 gaagatggtg agcacctttg tgatgaggtg gaaacctgtc atacgcaggg gattgcggat
     4801 gtcgttgtgc ccgtcgatga caaagctctg cgggattccg tggaggattt gggagcgctc
     4861 gaagcctttt tggatcttca cgctgttgcg ggcgtcagca atcacctggt ccatgtgttc
     4921 ctgcacgatt tcgccaggct cgttggggct ggaggtgggg atggagatgc gtccgtcgcc
     4981 aatttgggtg cggatgtggt ctccggtgac gttggtgaag atattggcag gtttggtgcc
     5041 ggcggcggtt tgcacttcct tcagggcttt tttaatgcct tcggacacgg cctggatgtc
     5101 tttcacgatg cctttttcaa ggccggtgga aggggtggcc gcgatgccga ggatatccaa
     5161 ctggcctttc gccttggctt gggccaggat gcaacggatg ttggca
//
LOCUS       DAOOWR010000094         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16076_length_5197_cov_3.316608, whole genome shotgun sequence.
ACCESSION   DAOOWR010000094 DAOOWR010000000
VERSION     DAOOWR010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16076_length_5197_cov_3.316608"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLB85_03375"
     CDS             <1..235
                     /locus_tag="PLB85_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU72399.1"
                     /translation="NLIVIFFFIPVATSNTRSKSRGLVFLLGLVVCFLYLIMVRVAQS
                     LGYSGVIPPVWAAWLPNLIFTLLGLAFLRKAEI"
     gene            313..2124
                     /locus_tag="PLB85_03380"
     CDS             313..2124
                     /locus_tag="PLB85_03380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU72400.1"
                     /translation="MLDSLRKNQKIIVYVVAIAFILSLGAGGIFGGERLLESFRGRYL
                     GKVNGTTISPQEYNAKVQEVVKRYEDQGQKVDETMMSYVYDTAWRELVDNALWQQQIK
                     KHRIKVSDTEIKNAMENDIPAELQQNPNLQTNGRFDKSKYFAALNNNFELRAAMYQSM
                     KEYLPKKKLQDKIKKEAKITVDSLKAEFIKESDTVTGKAIWFDFNMADNAAVNVTDAE
                     VKKYYEDNKDKEFKKGPATRLKYISIPIKPSEQDFNDVKLEIDDIYNQLQRDAASFAD
                     IAIEYSEDPGSADRGGSLGRFKRGQMVPEFDKVAFALKPGQISKPFRTQFGWHIVRCD
                     SVFPAPDGQEEVAASHILFKVNTTESSRSELRDQARDASKLIAKKGIDKAAKELKLEV
                     TTSRWLAHDNPQMEGFGEHGGLFQFMKSKKAGKVSEPFVLKNGGENIYVVAQVLDNKK
                     SYYEDFAEKRLQIKFDLEKKKKIASVKAKAENFVKRVPKENYLTAAVEEGWKVIDLNN
                     HKAKTRLAPPVNAQLDDFDKAALALNSGGWSSLLTTKEGPFLIFAEIRNRPNMQAFDK
                     VKQQEIRDSLEDAAFNRWFQQLRKDAKIIDNRYKFGY"
     gene            2131..3504
                     /gene="glmM"
                     /locus_tag="PLB85_03385"
     CDS             2131..3504
                     /gene="glmM"
                     /locus_tag="PLB85_03385"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU72401.1"
                     /translation="MSKLMTGVSGVRGVFGDALGPEVVLRYAARFGSFISQNSLSPAR
                     GAKPKIVVGRDSRTTGVAMLNCVTAALLSVGCDVIDLGIVATPTVLLNVKKHSAQGGI
                     SITASHNPPEWNALKFVDGDGMFLSPEKAAAFLDSVMEPLAWNGWEKMGRLSEDTEGT
                     AHHLEKVLGIPWLDLERICARNFKVVIDSVNGAGGLISPLLLKALGCEVIAINSAPTG
                     VFAHPAEPLNENLAQLEEEVRRQKADLGFATDPDVDRLSIVDEHGACIGEEYSVALAE
                     LFILPKNPGPIVVNLSSSMLSDHIAGLFGVPVHRAKVGEINVGKLMRELKSPVGGEGN
                     GGVICPEVNYTRDAVAGMALILGLLAESGKTVSQLVDELPRFYFAKGRLELPPERMND
                     AMTSLPGLLEAYELDLRDGVKATAPDHWIHIRKSGTEPIIRIYVESPSLERSTQLCQD
                     LIAKLSV"
     gene            4050..4772
                     /locus_tag="PLB85_03390"
     CDS             4050..4772
                     /locus_tag="PLB85_03390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU72402.1"
                     /translation="MKDAIGLLNRIEAANVLDAATGRGDFITILKQHLKSYIQIIGID
                     ASEKSVDQAQKRFPENDVEIYRMDLEALNFDDDSFDLVTIANSLHHIERLDRVFAELL
                     RVLKPGGRLLVQEMYRDGEQTEPQITHIMMHHWLASIDRRFGVYHQETYTREQIQAIF
                     AKLGLNQVEQVDFYLPVDNPKAASNCENLKRNCVETFKRLESLTDAEELLDEGKRLVE
                     RINSVGCAGACSLLLSGIKKNV"
     gene            4790..>5197
                     /locus_tag="PLB85_03395"
     CDS             4790..>5197
                     /locus_tag="PLB85_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOU72403.1"
                     /translation="MSEIENLFELPQSQLTKLAKKYEIPGYTQLKGNNLVFKLLEFQA
                     AQEGLAFVTGCLEIMEDGFGFLRFPQNNYLPGRDDVYVSLTQIRRFGLKSGHMISGPA
                     RSPKEGEKYFALLRVDAVNYMAPTSMQDLRTWDE"
BASE COUNT         1350 a         1504 c         1297 g         1046 t
ORIGIN      
        1 aaacctgatc gtgatctttt tcttcatccc tgtggccaca tcgaacaccc gctccaaaag
       61 ccgaggcctg gttttcctgt taggcctggt ggtttgcttc ctctacctca tcatggtgcg
      121 tgtggcgcaa agccttggct acagcggcgt tatcccaccc gtttgggctg cctggctgcc
      181 caatctcatc ttcactcttc tgggtctggc cttcctgcgc aaagccgaga tctaaaaaaa
      241 accttgtaga aaaacggcct ttgaaaatat tggcttttca aagctaattt aacccatcat
      301 gggaggagat aaatgctcga tagccttaga aaaaaccaga aaataatcgt ctatgtcgtg
      361 gcgatagcct tcatcctcag ccttggtgcc ggcggcatct tcggcgggga aaggctgctt
      421 gaatctttcc ggggcagata ccttggcaag gtgaacggaa caacgatcag cccgcaggaa
      481 tacaatgcca aggtccagga agtggtcaaa cgctatgagg accagggcca gaaagtggac
      541 gagaccatga tgtcctatgt ttacgacacc gcttggcggg agcttgtgga caacgccctc
      601 tggcagcagc agatcaagaa gcacagaata aaggtttcag acaccgagat caagaacgcc
      661 atggaaaacg atattccggc cgaactgcag caaaacccca atctgcaaac caacggcagg
      721 ttcgataaaa gcaaatactt cgccgccctg aacaacaact ttgagctccg cgcggccatg
      781 tatcagagca tgaaggaata cctgcccaaa aagaagctgc aggacaagat caaaaaagaa
      841 gccaagatca ctgttgacag cctcaaagct gaattcatca aggaatctga caccgtcacc
      901 ggcaaggcca tctggttcga tttcaacatg gcggacaacg ccgctgtgaa cgtaacagac
      961 gcagaggtga aaaagtatta cgaagacaac aaggacaaag aattcaaaaa aggccccgcc
     1021 acgcgcctca aatacatcag catccccatc aaaccctccg aacaggattt caacgatgtg
     1081 aagctggaaa tcgatgatat ctacaaccag cttcagcgcg acgctgctag tttcgccgat
     1141 atcgccatcg aatattccga agaccccggc tcggcagata gaggcggatc cctgggcaga
     1201 ttcaaacgcg ggcagatggt cccggaattt gacaaggttg ccttcgccct caaacccggc
     1261 cagatttcca aacccttcag aacccagttc ggctggcaca ttgttcgctg cgacagcgtc
     1321 tttcccgccc ctgacggaca ggaagaggtt gccgccagtc acatcctctt taaggtaaac
     1381 accacagaat ccagccgcag cgaactgcgc gaccaggcca gagacgcctc caaactgatc
     1441 gccaaaaagg gcatcgacaa agcggcaaag gaactcaagc tggaagttac aacctcacgc
     1501 tggctggctc acgacaatcc tcagatggaa ggttttggcg aacacggcgg ccttttccaa
     1561 ttcatgaaat ccaaaaaagc cggcaaggtc tcggaacctt ttgtactcaa gaacggcggc
     1621 gaaaacatct acgtggtcgc ccaggttttg gacaacaaga aatcctatta cgaggatttc
     1681 gctgagaaaa gactccagat taaattcgac ctcgagaaaa agaaaaagat cgcatccgtg
     1741 aaggccaaag ccgaaaactt cgtaaagcgc gttcccaagg aaaactacct caccgccgcg
     1801 gtggaggaag gctggaaagt gatcgacctg aataaccaca aggcaaaaac ccgccttgcc
     1861 cctcccgtga atgcccagtt ggacgacttc gacaaagccg ccctcgccct caattccggg
     1921 ggctggtctt ccctcctcac caccaaggaa ggccccttcc tcatctttgc cgaaatccgc
     1981 aacagaccaa atatgcaggc cttcgacaaa gtcaaacagc aggaaatccg tgacagcctt
     2041 gaagacgcgg ccttcaaccg ctggttccag cagctccgca aggatgccaa gataattgac
     2101 aacaggtata aattcggtta ttaagcacta atgagcaaat tgatgaccgg cgttagcggg
     2161 gtccggggag tctttggaga cgccctgggc cccgaagtcg ttctgcgtta cgcggcacgc
     2221 ttcggaagct tcatttccca aaattccctt tcccccgccc ggggcgcaaa accaaaaatc
     2281 gtggtgggca gggattcccg taccaccggt gtggccatgc taaattgcgt gacggcagcc
     2341 ctgctcagcg tgggttgcga tgttatcgac ctcggcattg tggccacccc aaccgtcctt
     2401 cttaatgtta aaaagcactc cgcccaaggc gggatcagca tcacggcttc tcacaacccg
     2461 ccggagtgga acgccctcaa attcgtggat ggtgacggca tgttcctctc ccctgaaaaa
     2521 gccgctgcgt ttctggattc cgtgatggaa cccctggctt ggaacggctg ggagaagatg
     2581 ggccgcctga gtgaggacac tgaaggcacc gcccatcatc tggaaaaggt tttgggaatt
     2641 ccctggctgg acctggagcg gatttgcgcg cgtaacttca aggtggtcat cgattccgtg
     2701 aacggcgccg gcggcctcat ttcgccgctg ctgctcaaag ccctgggctg cgaagtcatc
     2761 gccatcaatt ccgcccccac cggcgttttc gcccatcccg ccgaacccct gaacgaaaac
     2821 ctggcccagt tggaggaaga ggtgcgccgc caaaaagccg accttggctt cgccaccgat
     2881 cctgatgtgg acaggctttc catcgtggat gaacacggcg cctgcatcgg tgaggaatat
     2941 tccgtggccc tcgcggaact tttcatcctc cccaaaaacc ccggccccat cgttgtgaac
     3001 ctttcctcct ccatgctttc cgaccacatt gccgggctct tcggagtgcc ggtccacagg
     3061 gccaaagtgg gcgaaatcaa tgtgggcaag ctcatgcggg aattgaagtc cccggtgggc
     3121 ggagaaggca acggcggagt catctgccct gaagttaact acacccggga cgccgtcgcc
     3181 ggcatggccc tcatcctcgg attgctggcc gaaagcggca aaaccgtttc ccaactggtg
     3241 gacgaactcc cccgctttta ctttgccaaa ggccggctgg aactgccccc ggaacgcatg
     3301 aacgacgcca tgaccagcct ccctggactt ttggaagcct atgaactcga cctgcgtgac
     3361 ggcgtaaaag caaccgcccc ggaccattgg atccacatcc gcaaaagcgg aaccgaaccc
     3421 attatccgga tttacgtgga aagcccttcc ctggagcgct ccacccaact ctgccaggac
     3481 ctcatcgcca aacttagcgt ttagtctttt aaagcatttt cccaagcaac tttaggctgc
     3541 tctttgtttg aactctagca gccattttat ttgaaagaga gccataaccg attcccgctt
     3601 caatgccctt cggtcaggcc ctgagcgcct gagtccatcg ctctcccacg cccctcgcac
     3661 aatcctcgca ccaaatgcgg gtataatacg ggaggcactt cagaaacgat atacagaccc
     3721 ttaatggcca gacagcaaag cttcttccca gccacacgct ggagtcgagc gaggaacgag
     3781 catcgactcc agcaatgaat gattttcccg atacaccctg tagttcatcc ccgtgggttg
     3841 tattgtcgtc catgctcact atgttcgcta tacgccagga taatggtaac cacgttcttt
     3901 ctaaatgcgc atgagtcatt aaaaagcttg actaaaaaca gcgggtaaaa atcaatagac
     3961 acgttctgta tagagttgcc cggaacagca gtcagcctga gattcaggcg attcatcatc
     4021 cctatcacca tcaagcgagg attatcctta tgaaagacgc tattggcctt ttgaatcgga
     4081 tcgaggccgc caacgtcttg gacgccgcca cgggaagggg cgatttcatc accatcctga
     4141 agcagcatct gaaatcctat attcagatca ttgggattga cgcgtctgaa aagagtgtcg
     4201 atcaagccca gaaacgcttt ccggaaaacg atgtggaaat ctaccggatg gatcttgaag
     4261 ccctgaattt tgacgatgac agcttcgatc tggtgaccat cgccaattcc ctgcaccata
     4321 ttgagcgtct ggacagggtt ttcgccgaac tgctgcgggt gctgaagccc ggcggcaggc
     4381 tactggtgca ggagatgtac cgcgacggcg agcagaccga accccagata acccacatca
     4441 tgatgcacca ctggctggcc tccatcgacc ggcgtttcgg ggtttaccac caggaaacct
     4501 acacccggga acaaatccag gccatcttcg ccaaactggg actgaaccag gtggagcagg
     4561 tggattttta tctgccggta gacaatccca aggctgcctc gaattgcgaa aacctcaaac
     4621 gcaactgcgt ggaaaccttc aagcggctgg aaagcctgac cgatgccgaa gaacttttgg
     4681 atgaagggaa gcggctggtg gaacgtataa acagcgtggg ctgtgccggc gcctgcagtt
     4741 tgctgctgag cggaataaaa aagaatgtat aaaccaagga gtataaataa tgtccgaaat
     4801 agagaaccta tttgaactgc cacaatccca gcttaccaag ctggccaaaa aatacgaaat
     4861 acccggctac acccagttga aaggtaacaa cctcgtgttc aagctgctgg aattccaagc
     4921 cgcccaggag ggtctggcct tcgttaccgg ctgcctggaa atcatggaag acggcttcgg
     4981 cttcctccgc ttcccgcaaa acaactatct gcccggacgc gacgacgtct atgtctcgct
     5041 cacccagatc cgcagatttg gcctcaagag cggccacatg atatccggcc cggcgcgttc
     5101 cccaaaggaa ggggaaaaat atttcgccct gctgcgcgtg gacgccgtga actacatggc
     5161 ccccacctcc atgcaggacc tccgaacctg ggatgaa
//
LOCUS       DAOOWR010000095         5163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16219_length_5163_cov_3.226703, whole genome shotgun sequence.
ACCESSION   DAOOWR010000095 DAOOWR010000000
VERSION     DAOOWR010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16219_length_5163_cov_3.226703"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..852
                     /locus_tag="PLB85_03400"
     CDS             46..852
                     /locus_tag="PLB85_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOU72404.1"
                     /translation="MIKLLFFGDVFGKPGRQILYDQLPGLKAEFGPDFIIINGENLAD
                     GRGLTEKTVKPLFQAGVDIVTGGNHLWDRSEAWDFISHNPSIVKPLNFPATAPGNTVC
                     TLRKSELELTVLTLCGQIYMPPCDSPFTALERWFEDNPGHEGRCVLVDFHAESTAEKR
                     ALGWFADGHISALIGTHTHIQTADEEILPQGTAYITDAGMTGPHDSVIGVRKDIILNK
                     FHHGLPSRYETSNLGLQMNAVYLELDPATGKAWKIERLRRHVKLTASEGE"
     gene            854..1762
                     /locus_tag="PLB85_03405"
     CDS             854..1762
                     /locus_tag="PLB85_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOU72405.1"
                     /translation="MEKILSGKPLAKLIRSRVKELIAEHGLSPKMLLIQTGSDPASAY
                     YVQNIVTSGAKLGCDVRLMNLPEDISQAYLQELIASANDDPDIHGIMLQKPLPHHLDE
                     ALAGENISPDKDIDCLNPVNQGRIVLNQDALPPCTPLAVLCTLRYYGIPVAGKHTVII
                     GRSAVVGKPLANMLLWKKSWANASVTVCHSRSDNLGELTRSADILIAALGKAEFLKPD
                     MIKNNSVLIDVGINAHNSADGKGSFVGDIDYNACFDKALAITPVPGGIGSVTTSLLWL
                     NLLKAALAAKGTNKKIDDFLDFIFKD"
     gene            1862..4225
                     /locus_tag="PLB85_03410"
     CDS             1862..4225
                     /locus_tag="PLB85_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72406.1"
                     /translation="MLTGCGKFGNKFANEEPTIAITSYEGFDDSALLAPYAETEFIFQ
                     QKIFWNATDSDGIITGFAYRVKDQNGNPIATPGNHYIDMTGDVTPQNVVDRFGVGWVL
                     HYKTNADQNIPLDETPNDRKTIWTSAKYAVINFPAADANGNPLTMESTFEVIAIDNRG
                     GITPLYTPPDPQDPKRSIAWRKFNATSLRPTCIVTTTKGNPNGGVVGSGIRLGFSMKD
                     EDPFIDEIPFKFEFRMMKINPADSTVIDGSQTEWIDTKTSANDTKMDRYLLTRFTTPP
                     LSFDVEDDVIKTLTKIEARAYDLAGVMSNQTLNSKITFAVKTGFRPRTVIYPQRTYAL
                     GDNHFIDYLDESTPEIMPFTIVGGSQRFATPFFVNMNNVKTAINSNNIKVWLRWGWRG
                     EYGIVQTTGPILWPPIGQESPYDKKVDTVLDRYTDKNYFSEITHFDLRLDGAPYNYPP
                     YWEDRVTDSDGKEWLRIPLYSPLGQSLVLTSLSNGEHSLEVRCVDLQGEVDPEPATYT
                     FTLVEPIAAANRSGVLVIDDDLNNNATSPDNLIQPKYEQMLSSYTGTKTFIRRTRVGV
                     PGDTYGDYRLRHLATSDLQQYKLVIYHSDNPSESGNLKNENDGLALYMRSGGNFVLSS
                     TSKLASTLQAFVLATQRTFLNYMGVRWVDPPAKFLSDALQNRAYFQKAIGVSPFQDIN
                     LQYAAGNEASFNPLVNNLQGLSTITYFHTLGANAEPIYRMGIKPTNYPVSPPSQADYD
                     LYNNQPIALRTVTGNTHTYIFGFPLSYMMVDEAKAAMTQILTECGMI"
     gene            complement(4721..>5163)
                     /locus_tag="PLB85_03415"
     CDS             complement(4721..>5163)
                     /locus_tag="PLB85_03415"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOU72407.1"
                     /translation="PQRIVPAHTVKELVQSGEIVIAVGGGGIPVYVQDDGTYEGVDAV
                     IDKDFASALLAVNIDADLFVILTGVDKVSVDYGKPSQRDLDMMSMEEASCYYQEGQFP
                     AGSMGPKIKAAIDFLERGGKEVLITSIDRIVDAFAGKTGTRIVR"
BASE COUNT         1305 a         1573 c         1206 g         1079 t
ORIGIN      
        1 caaggtgaca gtgatccgcg aaacccgcca aatcgccatg gccaaatgat caaactcctc
       61 ttcttcggcg acgttttcgg caagcctggc cgccaaatcc tctatgacca gctgcctggc
      121 ctaaaagccg agttcggacc ggatttcatc atcatcaacg gtgaaaacct tgccgatggg
      181 cgaggcctca ccgaaaaaac agtgaaaccg ctcttccaag caggagtgga cattgttacc
      241 ggcgggaacc acctctggga ccgtagcgaa gcctgggatt tcatcagcca caacccctcc
      301 atagttaagc ctctcaattt tcccgccacg gcgcccggaa acactgtctg taccttgcgc
      361 aagagtgaac tggaactaac cgtgctcacc ctctgcggcc agatctacat gccaccctgc
      421 gattcgcctt ttacggcttt ggagcgctgg tttgaggaca atcccggcca tgaggggcgc
      481 tgtgtccttg tggatttcca cgctgaatcc accgctgaaa aacgcgccct gggctggttc
      541 gcagatgggc acatctcagc tctaatcggc acccacaccc acatccagac agccgacgaa
      601 gaaatcctgc cccaaggcac ggcttatata actgacgccg gtatgaccgg gccccacgac
      661 agcgttattg gcgttcgcaa agatataata ttgaataaat tccaccatgg cctgccctca
      721 cgctatgaga cctcaaacct cggattgcag atgaacgcag tctatctgga gcttgatcca
      781 gccacaggca aagcatggaa aatagaaagg ttgcgccgcc acgtgaaact aactgcctct
      841 gaaggtgaat gacatggaaa agatactatc gggaaaaccc ctcgccaagc tcatccgctc
      901 ccgggtcaaa gaactgatag cggaacacgg gctcagcccc aaaatgctgc tgatccagac
      961 aggcagcgac ccagcctcag cctactatgt gcaaaatata gtcaccagtg gagcgaagct
     1021 tggttgcgac gtgcggttga tgaacctgcc tgaagacatc agccaggctt atctgcaaga
     1081 gctcatcgcc agtgccaacg atgatcctga catccacggc atcatgctgc aaaagcccct
     1141 tccccatcac ctggatgaag ccctggctgg cgaaaacatc tcccccgaca aagatatcga
     1201 ctgcctgaac cctgtaaacc aaggcaggat agttctcaac caggatgccc tgccgccctg
     1261 cactccgctg gctgtgcttt gtaccctccg ctattatggg atacccgtag ctggcaaaca
     1321 caccgtgatc atcggccgca gcgctgtggt aggcaagccc ttggcaaata tgctgctctg
     1381 gaaaaaatcc tgggccaacg ccagcgtcac agtctgccat tcccgcagtg acaaccttgg
     1441 cgaactcacc cgcagcgcgg atatactcat cgccgccctc ggcaaagcag aattcttaaa
     1501 accggatatg atcaaaaata attccgttct tatcgatgtg ggaatcaatg cccacaattc
     1561 cgctgacggc aaaggctcct tcgtgggtga tattgattat aacgcctgct ttgacaaggc
     1621 cttggccatc actcccgtac ccggggggat tggctccgtg accacttcac tgctttggct
     1681 aaacctgctt aaagcagctc tcgcggcaaa gggaacaaat aaaaagattg acgattttct
     1741 cgacttcatt tttaaggatt agcagtaaga taatatccac cgatcatata tgggaggaat
     1801 gatgccaaat aagagcagta gactcttaac ccttttaatc gtattgactt tggcaatgct
     1861 gatgctcacc ggatgcggca agtttgggaa caaatttgcc aatgaggaac ctaccattgc
     1921 catcacatct tatgaaggtt ttgatgacag cgccctgctt gcgccctatg ccgaaacaga
     1981 atttatattc cagcagaaaa tcttctggaa cgcaaccgat agtgacggca tcatcactgg
     2041 ctttgcctat cgcgtgaaag accagaatgg aaacccgatc gctacaccgg gcaaccacta
     2101 catagacatg accggcgacg tgacccccca gaacgtggtg gatcgcttcg gcgtcggctg
     2161 ggtgctccat tataagacca atgccgatca aaacatccct ctggacgaga ctcccaacga
     2221 ccgtaagacc atctggacct cggcaaagta tgccgtgatc aatttccccg cggctgatgc
     2281 caacggcaat ccccttacca tggaaagcac ttttgaagtg atcgccatcg acaaccgggg
     2341 cggtattacc ccgctctaca ccccacctga tccccaggat cccaaacgca gcattgcctg
     2401 gcgtaaattc aacgccacct cactccgccc cacctgcatc gtcaccacca ccaagggcaa
     2461 tcccaacggt ggcgtggtcg gctccggcat ccggctgggc ttcagcatga aggacgaaga
     2521 ccccttcatc gatgagatcc catttaagtt cgaattccgc atgatgaaga tcaaccccgc
     2581 cgacagcacg gtgattgacg gctcccagac cgaatggatc gataccaaaa cttcggccaa
     2641 cgacaccaaa atggacagat atctgctcac ccgctttact actcccccac tcagcttcga
     2701 cgtcgaggat gacgtgatta agaccctcac caagatcgaa gcccgcgcct atgacctagc
     2761 cggtgtgatg tccaaccaaa ccttgaactc caagatcacc ttcgctgtca aaaccggttt
     2821 ccggccccgc actgtgatct atccccagag gacctacgcc ctcggtgaca accattttat
     2881 cgactactta gacgaatcca ccccagaaat catgcccttc accattgtgg gcggtagcca
     2941 gagatttgca acgcccttct tcgttaacat gaacaacgtt aagacagcca tcaatagcaa
     3001 caatatcaaa gtctggctgc gctggggctg gagaggcgaa tacggaatcg tgcagaccac
     3061 aggaccgatc ctctggccac ctataggaca ggaaagccct tacgacaaga aagtggacac
     3121 cgttctggac cgttatactg ataaaaacta ctttagcgag atcacccact tcgacctccg
     3181 cctcgatggc gcaccctaca actatccccc ctattgggaa gaccgcgtaa ccgacagcga
     3241 tgggaaggaa tggctgcgaa tccctctcta ttcacctctg ggacaatctc tggttctcac
     3301 ctctctttcc aacggcgagc atagcctgga ggtacgctgc gttgacttgc agggagaagt
     3361 tgaccccgaa ccggccactt acaccttcac ccttgtcgag cccatcgctg ccgccaaccg
     3421 tagcggcgtg ctggtgatcg acgacgatct taacaacaac gccacctccc ctgacaacct
     3481 gatccagcct aaatacgagc aaatgctctc ctcttacaca ggcaccaaaa ccttcatccg
     3541 ccgcactcgc gtcggcgttc ctggcgacac ctatggagac taccgcctcc gccatctggc
     3601 aacttcggac ttgcagcaat acaagctggt gatctaccac tccgacaacc ccagcgaatc
     3661 aggaaacctc aagaacgaaa atgacggact ggccttatat atgagatcag gaggcaattt
     3721 tgtcctctcc tccacttcca aactggcctc caccttgcaa gctttcgtgc tggcaaccca
     3781 gaggaccttc ctgaattata tgggagtccg gtgggttgat ccgccagcca agttcctcag
     3841 cgacgccctg cagaaccgcg cctacttcca gaaagcgatc ggcgtttcac ccttccagga
     3901 tatcaacctg cagtatgccg caggcaatga agccagcttc aatcccttgg taaacaatct
     3961 gcagggcttg tcaaccatca cctatttcca caccttgggt gccaacgctg aaccgatcta
     4021 ccgaatgggc atcaaaccca cgaattaccc tgtaagtccg ccttcccaag ctgattatga
     4081 tctatacaac aaccaaccca tcgccttgcg caccgttaca ggaaacaccc acacctatat
     4141 cttcggtttc cccctctcct acatgatggt ggacgaagct aaagcggcga tgacccaaat
     4201 ccttacggaa tgcgggatga tctaagcaag tacgaacaat aatacatatc accggctgcc
     4261 tccatggcag ccggtttgtt ttaggctggc gttaagatag gaatgagcat tggcgatagc
     4321 cacatagaac atagctacag cgacgccccc tctcacggaa ggtcgacgcc ccgtcgacca
     4381 aaatcccgga aggctttgca gaacctgacc aaaccctaaa cctggtaacg atgacatagc
     4441 ttgaatcgct cttctggagc gaggttaact cctcctttgc cctttcccat tcacatccca
     4501 ttcacttccc gctgagttcc cgccagtcaa acgggaagtc aaggggaggt gaaaaggagg
     4561 cgagtgggat ggggcaaagg gcctgatgcc cccggagacg ggagtgaagt tcctgccatt
     4621 tttttccgcc tccagttata gtggataatg atatcctttt acgcagacaa aaaagcccgg
     4681 aaccaaaagt cccgggctga tatcgtatgc tgaggctgcg ttagcgcact attctggtgc
     4741 ctgttttccc cgcgaaggca tccacgatgc ggtcgatgga ggtgatcagc acttcctttc
     4801 ccccgcgctc gaggaaatca atcgcggcct tgatcttggg acccatgctg ccggcgggga
     4861 actggccttc ctgataatag cagctggctt cctccatgct catcatgtcc aggtcgcgct
     4921 ggctgggttt gccgtaatcg acggacacct tgtccacacc ggtgaggatc acgaaaaggt
     4981 cggcatcgat attaacggcg aggagggcgc tggcgaaatc cttgtcgatc acggcgtcca
     5041 cgccttcata ggtgccgtcg tcctggacat agaccgggat tccgcctcca ccaacggcga
     5101 tcacgatttc gccgctctgg acaagctctt tcacagtgtg ggcgggaaca atcctttgcg
     5161 gaa
//
LOCUS       DAOOWR010000096         5113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16460_length_5113_cov_3.188612, whole genome shotgun sequence.
ACCESSION   DAOOWR010000096 DAOOWR010000000
VERSION     DAOOWR010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16460_length_5113_cov_3.188612"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLB85_03420"
     CDS             <1..525
                     /locus_tag="PLB85_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU72408.1"
                     /translation="KSRLGDIPAGVIQFETGSSFSIRDLVIHPFSSPHDAADSCNFTF
                     TRAGDEERKLGVATDLGFPSTLPLNKLKRCTTLVLESNHDERMLMDGPYDWALKQRIK
                     SNIGHLSNLQAVGMVSQVMHHGLQNLVLAHLSETNNDPQLALETMRNYLETVRSDIRL
                     LVAGQNGHTPLLDI"
     gene            complement(577..1725)
                     /locus_tag="PLB85_03425"
     CDS             complement(577..1725)
                     /locus_tag="PLB85_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOU72409.1"
                     /translation="MKRFTYQDKGSYFAIVAGSLERHAAAELGELGADVSRDVPRGLY
                     FCCDQETLYRVLYTSRLVQRVLAPLTSFACPDEKLLYRKARDLIDWAALFPVDTSFGI
                     QANVSSSKIRHSLYAGQVLKDAICDSFRDKTGRRPDFSTRLAPVNFNLHIHADRAAIS
                     LDLTGVSMHKRGYRKAAGAAPLQETLAAAMVRLSGWDGGRKLLDPMCGSGTILAEALM
                     SFCRIPAAFLRLDSGYQFLPDHHPELMKKVKNEADSEIRPLPEGLLKGSDMNARNIQT
                     AKENLALLPGGDKVELRTSRFQDLERESGRCVISNPPYGVRLEDRSRAERLYNDLGDF
                     LKQKCSDSESYLLCGDSRLVPQLRLRAHWRKNLKNADLEVVLAKILVR"
     gene            2336..5071
                     /locus_tag="PLB85_03430"
     CDS             2336..5071
                     /locus_tag="PLB85_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72410.1"
                     /translation="MNRFLLVLAVMLSAIALFAQTGIPVDQGTPELSPEILELIARNN
                     SQTRSFSFNFDSSPYNLPDAIDIDDPDTGLFLEDVAWTKDFTRHIDNPESIALTLSFL
                     YPSTRFTIIQPDPGNPLKLQFVPITANWYGSESLVLVATDPISSTEVMAVIRVNVTPV
                     PDPPVWSGLPDGNIFVTNEETALQIEFQDYVSCIDSADPTNFDLSVLFADFPITVTQE
                     PPGTGSLVTFTPHLDYNGMATYEITAVDRLSNAYSSQTIYINVLGVNDPPEIDSWQPV
                     ELTQNIDQGALLAFSVTASDVENDELTYTWTHSGIHNGVPFSDVVSTTSSLDWQFNIP
                     GTQSISCEVSDGQATDSVVWSVIVRPNGPLFNPPGGTYTSGISVTLAPPAGFEGAVIY
                     FTTDGNDPTEPSEVYTAPIAVDALPNAENEVAIKAFFTYPGFPPSQIESESYRITGTL
                     PEPGFMPLGGLFYAPVEVTLSNANPAAVIYYTLNGNDPSPGMPGTLEYELPIQVGAET
                     TVTIKALATREGWMNSTVASQTYIVTGTVQIDSHTMTPSPLPEGEFYLIDQGQYLAVE
                     IGGLSVTPASATVYYTLDNSLPAPGNPSAQVYTSGQQIQLTNPTWITFKAYLDDWQAS
                     DTYSYYYDVRAKTVLLDYANGTVFDPAPGYSTTPVSMSISSSTLPSGAAVYYTLDGSD
                     PDPATSQLYSGPILIEETTTIKALAQYPGLYPSDIQTGLFTITGTVAAPVFDPLPGSF
                     SSAIQLNMSSSTPDAAIYYSLDGSEPAPNATGSFLYEDPVDLGNGLHQVRARAFKEFW
                     DPSTITEGTYSIGILPAPEFDLAEGLYWDPIVVHLSVPGAPAASIHYTTDGSNPTEFS
                     TLYNPGTGIPIGLQTSYIIKAIAVQAGWINSAVTQRAYQVTGTVTTPAFTPDGGT"
BASE COUNT         1162 a         1542 c         1316 g         1093 t
ORIGIN      
        1 aaaagccgcc tcggagatat tcccgccggg gtcatacaat tcgaaaccgg cagcagcttc
       61 agcatccgtg atctggttat ccatcctttc tcctcacccc acgacgccgc ggacagctgc
      121 aacttcacct ttacccgggc tggcgacgag gagcgcaaac tgggcgtggc caccgatctg
      181 ggatttcctt ccacgctgcc cctgaacaag ctgaaacgct gcaccaccct tgttttagag
      241 agcaaccacg atgagcggat gctgatggac gggccttatg actgggcttt gaaacagcgc
      301 atcaaaagca acatcggaca tctttccaac ctccaggccg tcggcatggt gagccaggtg
      361 atgcatcacg ggctccaaaa ccttgtcctg gcccacctca gcgagaccaa caacgatcct
      421 cagcttgccc tggagaccat gcgcaactat ctggaaaccg ttcgcagcga catccggctg
      481 ctggtagctg ggcagaacgg acacaccccc ctgctggata tttaaccgat aaattaactt
      541 tccaagtcct tgatagcgcg tgggcaacaa tgttgtttag cgcaccagga ttttcgccag
      601 cacaacctcc aggtccgcgt tcttcagatt ctttctccaa tgcgccctca gccgcaactg
      661 aggcaccagc ctgctgtcgc cgcagaggag gtaggactcg ctgtccgagc acttttgctt
      721 gaggaaatcc ccgaggtcgt tatagagccg ctccgctctg ctcctgtctt ccagccttac
      781 cccgtaaggc gggttggaga tcacgcagcg gccgctttcc cgttcgagat cctgaaagcg
      841 tgaggttcgc agttcaactt tgtccccacc cggcagcaag gccaggtttt ccttcgcggt
      901 ctgaatgttc cgggcgttca tgtccgagcc ttttaacaat ccttcaggca gggggcgaat
      961 ctccgagtca gcttcatttt tgaccttctt catcaattcc ggatggtggt ccggcaaaaa
     1021 ctggtatccg ctgtccagcc tgagaaacgc cgccggaatc ctgcaaaagc tcatcaaagc
     1081 ctcagccagg atggtcccgc tgccgcacat ggggtccagc aattttctgc caccatccca
     1141 gcctgacaat ctgaccatcg ccgcggccag ggtttcctgc aatggcgcag ccccggcggc
     1201 tttgcggtag ccgcgtttgt gcatgctcac gccggtgagg tcaagcgaaa tggcggcccg
     1261 gtctgcgtga atgtggaggt taaaattcac cggagccagc ctggtgctga aatccggccg
     1321 ccgccctgtt ttatctctga agctgtcgca gatggcgtct ttgagcactt gccccgcgta
     1381 gagcgagtgc ctgatcttgc tggaactcac gttggcctgg atcccaaaac tggtgtccac
     1441 agggaacaga gcggcccaat caatcaggtc ccgcgctttg cggtaaagca gcttttcgtc
     1501 gggacaggca aaggatgtca gcggcgccag caccctctga accagcctgg aagtgtacag
     1561 cacgcggtaa agggtttcct gatcacaaca gaaatatagc cctcggggca catcccggct
     1621 cacgtcggcc cccagttcgc ccaattccgc agccgcgtgg cgttccaaac ttcccgctac
     1681 aatggcgaaa tagcttccct tgtcctgata tgtgaatctt ttcatcgggc cagtgggacg
     1741 gacagagcca ggctgtcaat caaaatcgtt tggtttctga aaatcactgg ccggaaatcc
     1801 cgctgaagag cgcagcgaag ctgtctggcc gttaaggccc tttcacaaat tccggatgtg
     1861 cctcccgcac gatgcccgca tttggtgcga ggatttggcg ggaggcgggg gagagggatg
     1921 ggcaagaggg ctaagggtgt ggcagacagg acatggcggt tggaaaacat atctggctct
     1981 tttacgaagc aagtagcggc gaaaatccaa tctgatagcg gtcaggaagg ctttatccat
     2041 cctgattgaa atgagcagga aactatcgga tttgttgccg atatccagct ttgcagacca
     2101 agggacggca gaatccatcg gcggatgagg ctggaattat ttctacaaga ggaagcgggg
     2161 cggaagaaaa attcattgac agcattaaaa ggggcgataa aatgacatta tgagtaaaag
     2221 tccgtattcg caccgcttgt tttcaggacc caacactctg gtgtttcgcc cggcgcaacg
     2281 gagcgcatac attgtcaaaa cacagccaag cgcaattctt ggaaggagat agagaatgaa
     2341 tagatttttg ttggttttgg ccgtgatgtt gagcgcaatc gctctcttcg cgcagaccgg
     2401 gattccggtt gaccagggaa ccccggagct ttcaccggag attctggagc ttattgccag
     2461 aaacaattcc cagacgagga gcttttcttt caatttcgac tcgtcgccat ataaccttcc
     2521 ggacgccatt gacatcgacg atccggacac aggtttgttt ttggaggatg tggcctggac
     2581 caaggatttc accaggcaca tagataatcc ggaaagcata gccctgacgc tttcattttt
     2641 atacccttcc actcgcttca cgataatcca gccggacccg ggcaatccgc tcaagctgca
     2701 gtttgttccg ataacagcca actggtatgg ttcggaatcc ctggttttgg tggcaaccga
     2761 cccgatcagc agcacagaag tgatggcggt gatccgcgta aacgttacac cagttcctga
     2821 tcctcccgtc tggagcggtt tgccggatgg caacatcttc gtgaccaacg aagaaacggc
     2881 gttgcagata gagtttcagg actatgtgag ctgcatcgac tcggccgatc ccaccaactt
     2941 tgacctcagc gttctgtttg ccgattttcc cataaccgtg acccaggaac cgcccggcac
     3001 gggttctttg gtcactttca cgccccatct ggactacaac ggcatggcga cctacgaaat
     3061 cacagccgtg gacaggcttt cgaacgccta cagcagccag accatatata tcaacgtgtt
     3121 gggggtaaac gacccgccgg agatagactc ctggcaacct gtagaactta cccagaatat
     3181 cgaccagggc gctttgctgg ccttttccgt gaccgcgtca gacgttgaaa acgacgaact
     3241 gacctacacc tggacccaca gcggcatcca caacggcgtg cctttctccg atgtggtttc
     3301 caccacatcc agtctggact ggcagtttaa catccctggc acacagagca ttagctgtga
     3361 ggtttctgac ggccaagcca cagacagcgt tgtctggagc gtgatcgtgc gcccaaacgg
     3421 accgttgttc aatcccccgg gcggcaccta caccagcggg atcagcgtta cgctcgctcc
     3481 cccggcaggt tttgaagggg ccgtgatcta tttcacaacg gatggcaacg atccaacgga
     3541 accatctgag gtatatactg ctccaatcgc agttgacgcc cttccgaacg cggaaaacga
     3601 agtggccatc aaggctttct tcacttatcc gggttttccg ccttcacaga tagagagtga
     3661 aagctaccgg atcaccggaa cccttcccga gccgggattc atgccactgg gcgggctttt
     3721 ctatgctccg gtggaagtta ctctgagcaa cgccaacccg gctgccgtga tctactacac
     3781 gcttaacggc aacgatccca gccccggaat gccgggcacc ctggaatacg aactgccgat
     3841 ccaggttgga gcggaaacaa ccgttaccat caaagctttg gccacaaggg aaggctggat
     3901 gaacagcacg gtggcttcac aaacctatat cgtgacaggc acggtgcaga ttgatagcca
     3961 caccatgacg ccttcgccgc ttccggaagg ggagttttat ctcatcgacc agggccagta
     4021 tctggcagtt gagattggcg gactgagcgt taccccggcc agcgcgacag tttattacac
     4081 ccttgacaac agcctgcccg ccccgggcaa ccccagcgcg caagtctata caagcggaca
     4141 acagatccaa ctcacaaacc ccacttggat caccttcaag gcctacctcg atgactggca
     4201 ggccagcgat acttacagct attactacga tgtgcgcgcc aaaacggttt tacttgacta
     4261 tgccaacggc acggtgttcg atcccgcacc gggatacagc acaacgccgg tttccatgag
     4321 catcagctct tccaccctgc cctccggcgc ggctgtttac tacacgcttg acggcagcga
     4381 tcctgacccc gctacctcgc agctttactc cggacccatc ctgatcgagg aaaccaccac
     4441 catcaaggcg ctggcccaat atccaggcct ctatcccagc gacatccaaa ccggtttgtt
     4501 cactatcaca ggcaccgttg ccgctcctgt tttcgatccc ctgccgggaa gcttttcctc
     4561 cgcgatccag ctaaacatga gctccagcac gcccgacgcg gcgatctatt acagcctgga
     4621 cggcagcgaa ccggctccga atgccacagg gtccttcctc tatgaagacc cggtcgattt
     4681 gggcaacgga ctgcaccagg tgcgggcccg cgctttcaag gaattctggg accccagcac
     4741 aatcacagaa ggaacctaca gcataggcat cctgcccgct cccgaatttg accttgctga
     4801 aggcctgtac tgggatccga tcgttgtgca tctcagcgtt cccggcgcgc cagccgcttc
     4861 catacattat acaaccgacg gctcgaaccc tactgagttt tccaccttgt acaatccagg
     4921 cacaggaatc cccatagggc ttcaaaccag ttacattatc aaggccatcg ccgtacaggc
     4981 tggctggatc aacagcgctg ttacccagcg agcttatcag gttaccggaa ccgtgaccac
     5041 gccggctttc acacccgatg gcggcaccta agccgtttcc actactgtcg ccatcacttc
     5101 ctaccccgcg gat
//
LOCUS       DAOOWR010000097         5100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16519_length_5100_cov_3.656293, whole genome shotgun sequence.
ACCESSION   DAOOWR010000097 DAOOWR010000000
VERSION     DAOOWR010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16519_length_5100_cov_3.656293"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="PLB85_03435"
     CDS             <1..2379
                     /locus_tag="PLB85_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU72411.1"
                     /translation="LRKQGVERLFEHQREAIDAALNGENLVISTGVASGKSLCYQFPI
                     LQEHLLNPTSRALLLFPTKALAQDQAQKMQNLATTLAHQNPKIPKLNCSIYDGDTASE
                     IRSRIRNQAGLVFSNPDMLHLGILPNHTLWSNFFAHLRWVVIDEVHIYRGVFGSHFAN
                     VLRRLKRICALYGAQPQFVCTSATVANARELAEDLLESPVRLIDRDSSPHGRREFLIV
                     NPPIVDATLGIRRSAMLESTSLAKRWLYGRGQAIIFCGPRRSVEILFLYLSGESAFKD
                     RVRSYRSGYLAAHRREIEKELREGSIGLVVSTNALELGIDIGGLDAVFLNTYPGTISA
                     TRQQAGRAGRKGNTALAILVASANPLDQYICQNPSYLFDNNPEHALISPDHREILQSQ
                     LLCAIHDLAMLDTEGFGSLGPEHIFPHLEVLVREGKVRKAGPRYIGVPEAYPAAEVSL
                     RNISDQVQILSGDELIGWVDGASALWMVHPGAIYLDRGETWLVTKLDLEQRKAEIEPA
                     EVNYFTQTTRDTEIEVNSLMNRQTALGADKFLGQVTVTTTITGFKKLKFYTQEIIGRE
                     PLDLPPTRLQTVAWWIGLNDKTVARVKTQGLWNVEKNDYGKDWGLISATARKRDNFTC
                     QHCGLLETGEAHHVHHLVPFRKFASTEEANVLENLVTLCPRCHRLAEQNVQMQSGIAA
                     LGYLLVNLAPFFVMCARKDIDVFCEDNSPLAGNRPVILIYDNISGGIGLSRKLFDLHD
                     QVLKAALDLVSKCPCHDGCPSCVGPVAENGAGAKEHALAILKELVANIPTGS"
     gene            complement(2835..3896)
                     /locus_tag="PLB85_03440"
     CDS             complement(2835..3896)
                     /locus_tag="PLB85_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72412.1"
                     /translation="MHSELKDYLLNEGKAPLDYVLDKFENHEIVLLGEMHYIRQQVEM
                     YHRLIPLLPEHGINLFATEFGRREDQALIDELLGKEQFDFPLAKLITLRQEAFWGYQE
                     YLDVYRLIWEQNRKNPPDRQIRCIGLNDPYNWKLYNKICREQKREPNAEERKLIWKDC
                     DEKNWLRALNKYHVPGVTKVLGIMGSHHAFTRYREPDFTEEGGRKVFAGFNKVRFGNQ
                     VHEEYGDAVCNICFYDPWDSLESDAPPKAPGGGIIEDIISPHFSELAFDLKDSPVGEL
                     ADDSFYSLGYKDFRLRDISDGMIYTCKLTEFKNLTPIPDFIDADNLQEFRNYAPFNYD
                     TDKSCEEINRIIAESAETW"
     gene            complement(4045..>5100)
                     /locus_tag="PLB85_03445"
     CDS             complement(4045..>5100)
                     /locus_tag="PLB85_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72413.1"
                     /translation="DGDGGLSPNWLGHKLILPSFEGSRACWAWPLNFSPKDLDPRSFD
                     FMPLRTANEKYWLMTGRGINPYYCFPLNSDDGTVTEEPRWGDTRFLNCLCGAQPTPTD
                     PEPAGRLNLAPGDNISYYSIYFVGAGLEGLRAQSQFIDSFIAGGLDIGPTSGLTCIPY
                     LTDFQVVDNQVARLDWHSFTDPHHFELKYKPWDAPASQWITRELPAQSRSVWIWDLDS
                     ALWYQIKLAAIYDPGPQEVYLESETLLTSLSQDSEPGIEIPPVELSLASFPNPFREKA
                     LVSFAVTADGPTRLQVFNSRGQLVRELLSEQKGPGTYQKIWDGRDAHGVSCSAGIYFL
                     RLESSGTSLIRKIALVR"
BASE COUNT         1170 a         1476 c         1344 g         1110 t
ORIGIN      
        1 ctgcgcaaac agggcgttga gcgtctgttc gagcaccaac gcgaggccat cgacgccgcg
       61 ctaaacgggg aaaaccttgt catcagcacc ggcgtggcca gtggcaagag cctctgttac
      121 cagtttccca ttctgcagga acatttgcta aaccccacca gccgggccct gctgcttttc
      181 cccaccaagg ccctggccca ggaccaagcc cagaaaatgc agaacctggc tacaactctg
      241 gcccaccaaa accccaaaat cccaaagctg aactgctcca tctacgacgg agacaccgct
      301 tccgaaatcc gcagccgcat ccgcaatcaa gccgggctgg tcttttctaa tcccgacatg
      361 ctgcatctgg gcattctgcc caaccacacg ctctggagca actttttcgc ccatctgcgc
      421 tgggtggtga tcgacgaggt gcacatctac cgcggtgttt tcggttcaca cttcgccaac
      481 gttttacgcc gtttgaagcg gatttgcgcc ctctatggag cccagcctca gttcgtttgc
      541 acctcagcca cggtggccaa cgcccgcgaa ctggcggagg atttgctgga aagccctgtc
      601 cgcctgatcg acagggattc ttcaccgcat ggacggaggg aattcctgat cgtgaaccca
      661 cccatcgtgg acgccaccct gggtatccgg cgcagcgcca tgctggaatc cacttctctg
      721 gccaaacgct ggctatacgg acgcggtcag gccatcatct tctgcgggcc acggcgcagc
      781 gtggaaatcc ttttcctcta tctgagcggc gaaagcgctt tcaaggaccg ggttcgcagc
      841 taccgtagcg gctatctggc cgcccaccgg cgtgaaatag aaaaggaatt gcgcgaaggc
      901 agcataggtc tggtggtttc caccaacgcg ctggaactgg gaatcgacat cggcgggctc
      961 gacgcggttt tcctgaacac gtatcccggc accatctccg ccaccaggca gcaggccgga
     1021 agggctggcc gcaagggcaa cacagccctc gccatcctgg ttgcaagcgc caatccgctt
     1081 gaccaataca tctgccagaa tcccagctac ctctttgaca acaatcccga gcacgccctc
     1141 atatcaccag accatcgtga aatcctgcaa agccagttgc tctgcgccat tcacgacctc
     1201 gccatgctgg atactgaagg ctttggcagt ttgggcccgg agcacatctt cccccatctt
     1261 gaagtgctgg tgcgggaagg gaaagtgcgc aaggctggcc cacgctacat cggcgttccg
     1321 gaggcctacc ccgccgcgga ggtctcgctg cgcaacattt cggaccaggt gcaaatcctc
     1381 tccggtgatg aactcatcgg ctgggtggac ggcgccagcg cgctctggat ggtgcatccg
     1441 ggcgctatct acctggaccg gggcgagacc tggctggtca ccaaactcga cctcgagcag
     1501 cgcaaggcgg aaatcgaacc cgcggaagtg aactatttta cccaaaccac ccgcgacacc
     1561 gagatcgaag ttaactctct aatgaaccgc caaacagccc ttggcgcgga caagtttctg
     1621 ggccaggtga ccgttaccac caccatcact ggcttcaaaa aactcaagtt ttacactcag
     1681 gaaataatcg gccgcgaacc actggacctg cccccaacga ggctgcaaac cgtggcctgg
     1741 tggatcggcc tgaacgataa aaccgtcgcc cgggtgaaga ctcagggtct ttggaacgtg
     1801 gagaagaacg actacggcaa ggactggggc ctgatctccg ccaccgcgcg caaacgtgat
     1861 aacttcacct gccagcactg cggcctgctg gaaaccggtg aagcccacca cgtccatcac
     1921 ctcgtcccct tccgcaaatt cgccagcacc gaagaggcca acgtgctgga aaatctggtg
     1981 accctctgtc cccgctgcca tcgcctcgcg gagcaaaatg tgcagatgca gagcgggatc
     2041 gccgccctcg gctatctgct ggtgaatctc gcgcccttct tcgtgatgtg cgcccgcaag
     2101 gacatcgacg tcttttgcga ggacaattcc cctctggctg ggaaccggcc tgtgatcctc
     2161 atttacgaca acatttccgg cggcattggc ctttcgcgca aactcttcga ccttcatgac
     2221 caagtgctga aagccgctct ggaccttgtt tccaaatgcc cctgccatga cggctgcccc
     2281 tcctgcgttg gaccagtggc ggaaaacggc gccggggcca aagaacacgc cctcgccatt
     2341 ttaaaagaac ttgtcgcgaa catcccaacc ggcagttagt ccccaacaca ctgtccatca
     2401 agtagatgag aacaaatccc caagcccatt gacactggcc tgaagacaag ccctttagct
     2461 gatatcctgc atgatgatta acaagcggct ctcttcctaa tcgggcgtat aacgcattgg
     2521 gatggaaaac gatagcgagg gtgtgaaatg actgtattcc ctcggaggag cgaagcgata
     2581 ccgtcaggcc gttaaggcct tgtcacttgt caaggattcg cctcccgtat gatggccgca
     2641 tttgatgcga ggattatgcg cgggaaatga aagaggaatg gaattgaccg ctcaggatgt
     2701 gacggacagg acattacagt ggatttataa ctctatatca aatcaagtgg atacaagtgc
     2761 tcaaacagaa agcagccaac agattattac ccacccggtt gtaaagagag cctaacaagc
     2821 tacaaagcct cccatcacca ggtttcagcc gattccgcga taatccggtt aatctcttcg
     2881 cagcttttat ccgtatcata attgaagggc gcgtaattac gaaattcctg caagttatcc
     2941 gcgtcgataa aatcggggat tggggtcaag tttttgaatt ccgttaactt gcaggtatag
     3001 atcatgccgt cggagatgtc tctcagccgg aaatccttat aacccaggga gtagaaacta
     3061 tcatcagcca gttcgcccac ggggctgtct ttcagatcaa aagccagttc agagaagtgc
     3121 ggactgatga tatcctcgat gataccaccg ccaggcgcct ttggaggagc gtcagattcc
     3181 agactgtccc agggatcgta aaaacaaata ttgcaaaccg cgtcaccgta ttcctcatgg
     3241 acttggttgc caaaacggac tttattgaat ccggcaaaca ctttgcgccc accctcctcg
     3301 gtgaaatccg gctcgcgata gcgggtgaag gcgtgatgcg aacccatgat acccaaaacc
     3361 ttggtgacgc ctggaacatg gtatttgtta agcgcgcgca gccagttttt ttcatcgcaa
     3421 tccttccaga tcagttttct ctcctcggcg ttcggctcgc gcttttgttc gcggcatatc
     3481 ttattgtaca gcttccaatt atacggatca ttcaagccaa tgcaacggat ttgcctgtcc
     3541 ggaggattct tgcggttttg ctcccagatc aacctataga cgtccagata ttcctggtac
     3601 ccccaaaacg cctcctgacg cagtgtgatc agctttgcca gaggaaagtc gaactgctct
     3661 ttgccaagta actcgtctat aagagcctga tcttcccttc gcccaaattc ggtggcaaac
     3721 aggtttatgc cgtgctccgg caaaaggggt ataagtcggt gatacatttc cacttgctgg
     3781 cggatgtagt gcatctcacc caaaagcacg atctcgtggt tctcaaattt atccaataca
     3841 tagtccagcg gtgctttgcc ttcgttcaaa agatagtcct tcagttcgct atgcatgatg
     3901 atttctcgct gttatttttc tttgagtgtt aaaactatct acaggtcgcc tatgacgtca
     3961 aggcttatta agtcctttgg gcttttagag acacatctcc aatagcaata accctatccc
     4021 gctgccagaa ggctggattt agctctatct taccaaagcg atttttcgga taaggctggt
     4081 gccgctgctt tccaaacgta gaaaatagat tcccgcggag caggatacgc cgtgggcgtc
     4141 ccggccgtcc cagatctttt ggtaagtccc agggcccttc tgttcagata gaagttctcg
     4201 cacgagctgg cctctggaat tgaaaacctg cagtctggtg ggtccatctg ccgtcacagc
     4261 gaagctcacc aacgcctttt ccctaaaggg attgggaaaa ctggcaaggc tgagttccac
     4321 cggaggaatc tcgatgccgg gttccgaatc ctggctgagc gaggtgagca gggtctcgct
     4381 ttcgagataa acctcttggg gacctggatc gtagatggcg gcgagtttga tctgatacca
     4441 gagggcggaa tcgaggtccc agatccagac ggagcggctc tgcgccggca attcgcgggt
     4501 gatccactgt gaggcaggag catcccaggg tttgtatttg agctcaaagt ggtggggatc
     4561 ggtgaaggaa tgccagtcga ggcgcgccac ctgattgtca accacttgga aatcagtgag
     4621 ataggggatg caggtgaggc cgctggtggg accgatgtcc agtcctccgg cgatgaaact
     4681 gtctatgaac tggctctggg cgcgtaaacc ttccaatcca gcgcccacga agtaaatgct
     4741 gtaatagctg atgttatcgc ccggcgcgag attcagccgg ccagccggct ccgggtcagt
     4801 gggggttggc tgagctccgc agaggcagtt cagaaaccgg gtgtcgcccc atctcggctc
     4861 ctcggtcacg gttccgtcat ctgagttgag ggggaagcaa tagtatggat taatgccgcg
     4921 tccggtcatt agccagtact tttcattggc ggtgcgcagg ggcatgaaat caaaggagcg
     4981 gggatcaagg tctttgggag aaaaattcag cggccaggcc cagcaggccc tgctgccctc
     5041 gaaactcggc aggatcagct tgtgccccag ccagttgggg cttagcccgc catcgccgtc
//
LOCUS       DAOOWR010000098         5093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16546_length_5093_cov_4.241366, whole genome shotgun sequence.
ACCESSION   DAOOWR010000098 DAOOWR010000000
VERSION     DAOOWR010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16546_length_5093_cov_4.241366"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..494)
                     /locus_tag="PLB85_03450"
     CDS             complement(45..494)
                     /locus_tag="PLB85_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOU72414.1"
                     /translation="MARTSESALQQEIMAWISQNQYVYLATVDKKQARVRPVVLFMYE
                     DRYFFATFSGDAKVGQISANKWVEVCVPLRESGNTGYIRLTGIARIVTNAALKAEASE
                     RCFFFDEYFHGYDDPDYTLIEFEADFAEYLRPGERTSQTCNLKRHTG"
     gene            complement(504..1361)
                     /locus_tag="PLB85_03455"
     CDS             complement(504..1361)
                     /locus_tag="PLB85_03455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU72415.1"
                     /translation="MLKTPQKIALVAMVLVLSLSLLNCQTNELKSAGAAKLEEKMDKE
                     TQVKTAVIETNQGNIELELWPNLAPKTVDNFLKLAAEGFYNQTYFHRVIPDFMIQGGD
                     PNTKDGDRSNDGMGGPGYQFEDECYAPGEAVTGEVNDEETAMLVWTKIIIPYMQSAKN
                     PQQEVFDIVRAVQEEQSPEPIYGKTVEWYQNRTGISEQLRKQILKAPVLYSYICMANS
                     GPNTNGSQFFIVTKQPGTPWLDGKHTVFGKVTSGMDVVHKIENLPRDKQDNPEVGNQA
                     IIKAINLTK"
     gene            complement(1279..2106)
                     /locus_tag="PLB85_03460"
     CDS             complement(1279..2106)
                     /locus_tag="PLB85_03460"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOU72416.1"
                     /translation="MRICVLSDVHYKYVFKDTYDRANANLALDFLKQAVGKYDLMVLN
                     GDIFDLWFDWKYAIIKQYFPILHRLAEIREHGCRLVLISGNHDFWFNDFFRDYLEIEV
                     YNNSFSLKEDGLRMLFTHGDLHTVNDLRYKFFRRLIRMKLSKKLFSLLHPDLALLIGG
                     KLSRSSRFRQVSSLLQSKKSAGLSRYAEYMIGKKDYDLVCMGHSHHPGITRLKGGSYA
                     NSGDWTRHHSYLEIDSGQVSLKYYKSKEMHNAQNPAEDRPGGDGPGSQPQPAELPDQ"
     gene            complement(2108..2779)
                     /locus_tag="PLB85_03465"
     CDS             complement(2108..2779)
                     /locus_tag="PLB85_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOU72417.1"
                     /translation="MPRNLLPAFLLILSTTLGAVTGNVTTTGFARLQYNGGGDWYNDP
                     EVLPNLARFVNASLNTTFPLDQSVVKASEAKLFEYPFVYLTGHGNIRWDDREVENLRQ
                     WMLRGGFLYADDDYGMDSSFRREIKRVFPEYELVELDGSSPLFTSFFDFSGGLPKIHL
                     HDEKPPQAFGIFDDDGRLMCLYTYETNISDGWADPATHKDPPEVRETALRFGCNIIYY
                     LLTRG"
     gene            complement(2781..4133)
                     /locus_tag="PLB85_03470"
     CDS             complement(2781..4133)
                     /locus_tag="PLB85_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU72418.1"
                     /translation="MIKLQSKYALSPQSICEHQSSQDGRYAWFLCVAHEENDIALEEI
                     LAGVKLEVLNAKVLEISKMEIENWLKSFFADFHWKLHASLRRTNLREKGLSLFFAVCF
                     DHDLIFVQFGRLFCAVTNGKKLETVGRNWKNYHVQSLKNLNLLGLSEEDIRVKPQKLH
                     LKENESLVVLPGKVAAKVFGSSPDVSSLQPLVESFSSASPALWLILKHQTPLAKPKKR
                     RLTKLEISTLFLLLGTALAIIYMALGNRIIGVGFYRVKKVVNEKIEQAITVLNMPARN
                     VQLVRNWSMELPFRVTSSPAFNQQNVFLVSGADIYAYKLSSREQLWKQNYPAPVGAIM
                     TTAAGLNATLANGNTLGLDLRGNEVWSQSLQALANEKANLTMLEITPAQEKRIDKNLT
                     VLPLRRGLAILDSQQGKLMSELALPEELRFISKYDDYNSCFYLVTQDSLVCVNLNIVN
                     "
     gene            complement(4135..>5093)
                     /locus_tag="PLB85_03475"
     CDS             complement(4135..>5093)
                     /locus_tag="PLB85_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU72419.1"
                     /translation="NVWKKKAHNFTVEEYAKTFKKIGRSPYWITFSGGEPFLRKDIVE
                     VVSTIYEISHPNIINIPTNGILTGTIVEKTAAIARACPKSQVIINISVDGIGEQHDEI
                     RKVPGNYKKTMATFQKLKALQIPNLAVGIHTVISQFNVDTFPGIANELLRLKPDAYIT
                     EIAEERGELDTFGAQITPSLVAYKSAIDYLIHRIKNERYKGMNKITMAFRIEYYNLVK
                     RIMRDERQILPCYAGVASAQISPDGDVWSCCVKAKSLGNLRASKYNFREIWFNHESEL
                     ERRSIHKKQCWCPLANAAYTNMLMDIPTLMRVFWRSMIKWWN"
BASE COUNT         1074 a         1295 c         1417 g         1307 t
ORIGIN      
        1 aggtgaaaat cgctttcatt atcaatcctg attctgctcc ggggtcaacc ggtatggcgt
       61 ttgaggttgc aggtctggct ggtgcgctcg ccgggacgga ggtattcagc gaagtcggct
      121 tcaaactcga tcaaggtgta gtccggatca tcatagccgt ggaaatattc gtcaaaaaag
      181 aagcagcgct cggaggcttc agccttgagc gctgcgttgg taacaatcct ggcaatgccg
      241 gtgaggcgga tgtagcctgt gttcccggat tcacgaagag gcacgcagac ctccacccac
      301 ttgttggcac tgatctggcc gaccttcgcg tctccgctga aggtggcgaa gaaatagcgg
      361 tcttcataca tgaatagcac cacgggacgt acccgggctt gttttttatc gaccgtggcc
      421 agatagacat actggttctg gctgatccag gccatgatct cctgctgcag ggcggattca
      481 gaggttcgtg ccatggtaga gccttattta gttaggttta tggccttgat gatggcctgg
      541 tttccaactt ctggattgtc ctgtttgtcc cggggcagat tttctatctt gtgcaccacg
      601 tccatgccgc tggtcacttt gccgaaaacg gtgtgtttgc cgtccagcca gggagttccg
      661 ggctgtttgg tgacgatgaa gaactgggag ccgttggtgt tgggtccgga attggccatg
      721 cagatgtagg aataaagaac gggggcctta agtatttgtt tccggagttg ttcgctgatg
      781 ccggtgcggt tctggtacca ctccacggtc ttgccataga tcggttccgg actctgctct
      841 tcctgcacgg ctcttacgat gtcgaagacc tcctgctggg gatttttggc cgactgcatg
      901 taggggatga tgatcttggt ccagaccagc atggctgtct cttcatcgtt gacctcgccg
      961 gtgaccgctt cacccggagc gtagcattcg tcttcaaact ggtagccggg cccgcccatg
     1021 ccatcattgg aacggtcgcc gtccttggtg ttggggtcac cgccctggat catgaaatcc
     1081 gggatgacgc ggtgaaagta ggtctggttg taaaagcctt ccgccgccag tttcaggaag
     1141 ttgtccaccg tcttgggcgc caggttgggc cagagttcca gctcaatgtt gccttggttg
     1201 gtttcgatca ctgcggtctt cacttgggtt tccttatcca ttttttcctc cagttttgcg
     1261 gctcctgccg acttcagttc attggtctgg cagttcagca ggctgaggct gagaaccagg
     1321 accatcgcca ccagggcgat cttctgcggg gttttgagca ttgtgcatct ccttgctttt
     1381 gtaatatttg aggctcacct ggccagaatc tatctccagg tagctgtggt gccgggtcca
     1441 gtcgccgctg ttggcataac ttccaccttt taagcgggtt atcccgggat ggtggctgtg
     1501 gcccatgcaa accaggtcat aatccttttt tccaatcata tactccgcgt aacggctcag
     1561 tcccgcactt tttttggatt ggagcagaga ggatacctgc cggaaccggc tggagcggga
     1621 tagctttccc ccgatcaaca gagcgagatc agggtgcaac agggaaaaga gcttcttgct
     1681 gagcttcatg cggatcaggc gtcggaaaaa cttgtagcgg aggtcgttca cggtgtgtaa
     1741 atcgccgtgg gtgaacagca tcctgaggcc gtcctccttc aggctgaaag agttattgta
     1801 aacctcgatt tcaagataat ctcggaaaaa atcattgaac cagaaatcgt ggttgccgct
     1861 gatcagaacc agcctgcagc cgtgttcacg aatctccgcc aggcggtgga gaatgggaaa
     1921 gtactgcttt atgatggcgt atttccagtc gaaccagagg tcgaaaatat cgccgttgag
     1981 caccatcaag tcgtattttc ccactgcctg tttcagaaag tcgagcgcca gattggcgtt
     2041 ggcacggtcg taagtgtctt tgaacacgta tttgtaatgc acgtcagaca gcacgcagat
     2101 gcgcatctca accccgggta agcaggtagt agatgatgtt gcagccaaag cggagagctg
     2161 tttcgcgcac ttccggcgga tccttgtgag tggcgggatc ggcccagccg tcgctgatat
     2221 ttgtttcgta ggtatataga cacatcaggc ggccgtcatc gtcaaaaatg ccaaaggctt
     2281 ggggcggctt ttcatcgtgc aggtggattt tgggcagacc gccgctaaaa tcgaaaaagc
     2341 tggtaaacaa gggtgaagag ccatccaact ccaccagctc gtattctgga aagacccgtt
     2401 tgatctcacg ccggaaagag gaatccatgc cataatcgtc atcggcgtag agaaagcccc
     2461 cgcgcagcat ccactggcgc aaattttcca cttcgcggtc atcccagcgg atgttgccgt
     2521 gcccggttaa ataaacgaaa ggatattcga aaagctttgc ttcgctggct ttgaccacgc
     2581 tttggtcgag gggaaaagtg gtgttcaggc tcgcgttcac aaaacgggcc agattgggca
     2641 aaacctccgg atcgttgtac caatcccctc cgccgttata ttggagccgg gcgaagcccg
     2701 tggtggtgac gtttccggtc acggcaccca aggtggtgga aaggatcagg aggaaggccg
     2761 gcagcaggtt tcggggcatc ttagttcaca atgttcaggt tcacgcagac cagggaatcc
     2821 tgcgtaacca ggtaaaaaca actgttgtaa tcgtcatact tggaaataaa cctaagctct
     2881 tccggcagag ccagttcact catcagtttg ccctgctggc tgtccagaat tgccagtccc
     2941 cgcctaagag ggagcacagt gaggtttttg tctatgcgct tttcctgagc cggcgtgatc
     3001 tccagcatgg tcaggttggc cttttcgtta gccagagcct gcaatgactg gctccagacc
     3061 tcatttcccc gcaaatccag ccccaaagtg ttgccattgg caagggttgc gttaagtccg
     3121 gccgctgtgg tcattatcgc gcccaccggg gcaggatagt tctgtttcca aagctgctcg
     3181 cgggaggaaa gcttataagc atagatatcg gccccgctga ccagaaacac attctgctgg
     3241 ttgaaagcgg gagaggatgt aaccctgaag ggcagttcca tgctccaatt ccggaccagt
     3301 tgcacgttcc gggccggcat gttcaaaacg gttatagcct gctcgatctt ttcgttgacc
     3361 actttcttga cgcggtagaa gcccacaccg atgatgcggt tccccaaggc catgtaaatt
     3421 atagccaaag ccgttcccag aagcaggaac agggtgctga tctccagctt ggtcagtcgg
     3481 cgttttttgg gtttggccag aggagtttga tgcttgagga tgagccacag cgccggagag
     3541 gcagagctga aggattccac cagcggctgc aggctgctca cgtcaggaga gcttccgaaa
     3601 accttggctg ccacttttcc gggcagcacc accagacttt cattttcttt cagatggagt
     3661 ttttgcggtt tcacccggat gtcttcttcc gaaaggccca gcagattcag attcttcaag
     3721 ctttgcacgt ggtaattctt ccagttccgg cccaccgttt ccagtttttt tccattggtg
     3781 acggcacaaa agagacggcc aaactgcaca aagatgagat catggtcaaa acacacagcg
     3841 aaaaagaggg acaggccctt ttcccgcaga tttgtgcggc gcaggctggc atgcagcttc
     3901 cagtggaaat ctgcgaagaa actcttgagc cagttctcga tctccatctt gctgatctca
     3961 agcaccttgg cattcaatac ttccagtttc accccggcca ggatctcctc cagggctatg
     4021 tcgttctctt cgtgggccac gcagaggaac caggcgtaac ggccatcctg ggaagactgg
     4081 tgttcacaga tgctttgagg agacagggcg tatttgcttt gtagtttgat cattttaatt
     4141 ccaccatttg atcatgctgc gccaaaaaac cctcatcagc gtgggaatat ccatcagcat
     4201 gttggtatag gcggcgttgg ccagtgggca ccagcactgt tttttatgaa tggagcggcg
     4261 ttccagttcg gattcgtggt tgaaccaaat ctcgcggaaa ttgtatttgc tggcgcgcag
     4321 attgcccaga gactttgcct tgacacagca gctccaaacg tcaccgtccg gcgagatctg
     4381 ggctgaagcc actccggcat aacagggcaa tatctggcgt tcgtcgcgca tgatgcgttt
     4441 caccagattg taatactcta tcctgaaagc cattgtgatc ttgttcatcc ccttgtatct
     4501 ttcgtttttg atgcggtgga tgagataatc gatggcggat ttataagcca caaggctggg
     4561 cgtaatctgg gccccaaagg tgtccagttc tcccctctcc tcggctatct ctgttatgta
     4621 ggcatccggt tttaggcgca gcaattcgtt ggcgatgcct ggaaaggtat ccacattgaa
     4681 ctgcgaaatc acggtatgga tgcccactgc cagattgggg atctgcaggg cttttagctt
     4741 ctggaaggtg gccatcgtct tcttgtagtt gcctggcacc ttgcgtatct cgtcgtgctg
     4801 ttcgccgatg ccgtccaccg atatgttgat gatcacctgg gacttggggc aggccctggc
     4861 gatcgcggcg gtcttttcca ctatggtgcc ggtgaggatg ccgttggtgg ggatgttgat
     4921 tatgttgggg tgtgaaatct catagatggt ggagaccact tccacaatgt ccttgcgcag
     4981 aaagggctca cctccgctga atgtgatcca gtaaggcgag cggcctatct tcttgaaggt
     5041 tttggcgtat tcttccactg tgaaattgtg ggctttcttc ttccagacat tac
//
LOCUS       DAOOWR010000099         5079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16611_length_5079_cov_4.329618, whole genome shotgun sequence.
ACCESSION   DAOOWR010000099 DAOOWR010000000
VERSION     DAOOWR010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16611_length_5079_cov_4.329618"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /gene="yidC"
                     /locus_tag="PLB85_03480"
     CDS             <1..1509
                     /gene="yidC"
                     /locus_tag="PLB85_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU72420.1"
                     /translation="SIKAPTVAEEPQIALPDSLIKGADSLRTLTLANDNFTVSFSNLG
                     ASITSIKLKKYNWADKKTNTPVDLVPQGRDLAGISLLQHKASPPKDLKMLYWQSAQSD
                     TAGIVFWLGEQSSPLVTKSYRLDDKYGILMDVKVNSPDAVYGIEYDFSAGIADSEVVK
                     PRAKDQDYKVLLFADNVPVKLTLPKIKKEAQSSPVSSFKWAALRSKYFSIAIHETGNA
                     LTSNYHPGIAKDTGNPSLILDSRDDRPSQTWAQSFLIYAGPADYDLMKSYEDTKLELI
                     PDRGPGWLRWLANAIAWLLKFLHSFIPNYGIVIIIFSFILKLILHPLTKKSMDANLKM
                     QSIQPQLQEIQTKYKNDPQRMQQEIAKAYKEAGTSPMSGCLPILLQMPIFIALYNVLR
                     YAMAMRNVSFLGLNLSEPDPWILPAIMAGFMILQSLMTRPSKEAMAKMDEKQQAMQQS
                     SRMMTWIMPVMLFFIFRGLPSGLVLYYTVFNMLSVAHQYYTQKRLKQKETIS"
     assembly_gap    1529..1538
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1539..2352
                     /locus_tag="PLB85_03485"
     CDS             <1539..2352
                     /locus_tag="PLB85_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOU72421.1"
                     /translation="SIEAIIAAFRGEHRIRDWELNYKIVKKPSKGLFGLFGRKQAVVR
                     FELPSLGERAGNLLSQLLGKMGIPFSRVTTKTEGKSIHLEIKDSQDTGLLIGKNGYML
                     ETLQYFVNRVFENEHDIDRIYIDVDGYRERREAQFLKKFQPIMKKVREQGKPQTLEPM
                     SSTDRRIIHRYVERERGLRTLTIGEGEQKRIVIFSARQKESEALSQAQDSKDKKPRSL
                     RRERGGRPKLNTRPEHSLKREQNLKPEQNPKPGQRPRPNRRPRPKGKTSAKA"
     gene            2349..3152
                     /locus_tag="PLB85_03490"
     CDS             2349..3152
                     /locus_tag="PLB85_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU72422.1"
                     /translation="MKPDISLHKIDAGNYWTDGGAMLGVLPRAIWGKSVQTDERHRKN
                     LALNLLLIKMADRVILVDTGLGNRLSEKQRDIYRPSAFLLPVSLAELGIRDADVTDVV
                     LTHLHFDHAGGIITGFGDHDALTFPKARHWIQCQEWETAKNPDGLNRASYDFPRQLAL
                     LEERGRQQLIDGSEEIAPGVTLVKTGGHTCGSQIVEINSAHGYFIYAGDIMPTLFHVS
                     PAVTSAYDVCREDTFKAKQYISARLKEKNGTLLLNHDPRHWELPASALK"
     gene            3487..4419
                     /locus_tag="PLB85_03495"
     CDS             3487..4419
                     /locus_tag="PLB85_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOU72423.1"
                     /translation="MIQYFKVREQRFEPALAHSEAFWIHLESPTADEIKALIEEFDLP
                     EDFITDLQDADETSRMEYEDNAVLVIMRVPLYYRHRSASLAFTTAPLGVIAVQDKLIT
                     VSFFDNEVLNVFLDCKHKPFRITQQSFLLQIALRISLYFLRFLKEINRRTNKIESELS
                     QSLRNQELFRLLRLEKSLVYFSTSLKSNEIILERLQRSRWLNQDPESEDLVEDVIIDN
                     TQAIEMANIHSSILSGMMDAFASIISNNLNVVMKFLTSVTIILSVPTLIASLYGMNVR
                     LPLEGNPHAFWAVIGISVLASLVLVLIFIRKKYF"
     gene            4429..4800
                     /gene="queD"
                     /locus_tag="PLB85_03500"
     CDS             4429..4800
                     /gene="queD"
                     /locus_tag="PLB85_03500"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOU72424.1"
                     /translation="MYKLSVTDSFSAAHRLCGYEGACSNLHGHNWTVRVALEAERLDN
                     IGMAMDFGHIKALLKEITDGLDHAYLNDLPAFSEQNPTSENLARHIFERMAETLAGKP
                     VRVAEVEVRESERSSVTYSHA"
     gene            4793..>5079
                     /locus_tag="PLB85_03505"
     CDS             4793..>5079
                     /locus_tag="PLB85_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOU72425.1"
                     /translation="MLRFVESFFVKSAVEPKDYPASAYPEFAVAGRSNVGKSTLINLL
                     TERRKLAKTSSHPGKTRLLNFFLIRWKDDDAGKEGCLQLTDLPGYGFAEVS"
BASE COUNT         1232 a         1557 c         1325 g          955 t
ORIGIN      
        1 agcatcaagg ctccgacggt ggccgaagaa ccccaaatcg cacttccgga cagcctgatc
       61 aaaggcgccg acagcctgag gaccctcacc ctggccaatg ataacttcac ggttagcttc
      121 agcaacctcg gcgccagcat cacctccatc aagctaaaga aatacaactg ggcggacaag
      181 aaaaccaaca cacccgtgga cttggttccg caaggcaggg acctcgccgg catctcgctg
      241 cttcagcaca aagcttcccc gccgaaagac ctgaaaatgc tttactggca aagcgcgcag
      301 agcgacacgg cgggaatagt tttctggctg ggcgagcaaa gctccccgct ggtgaccaaa
      361 agctaccgtc tggacgacaa atacggcatc ctgatggatg tgaaggtcaa cagcccggat
      421 gccgtgtatg gcattgaata cgacttcagc gccggcatcg ccgacagcga agtggtcaag
      481 ccccgcgcca aagaccagga ttacaaggtg ctgcttttcg cggacaacgt tccggtgaag
      541 ctcaccctgc ccaagataaa gaaagaagcg cagtcctcgc cggtatcgtc tttcaagtgg
      601 gccgctttgc gcagcaaata cttcagcatc gccatccacg aaaccggcaa cgccctcacc
      661 agcaactacc atcccggcat cgccaaagat accggaaatc caagcctgat tctggattca
      721 cgggatgacc gcccttccca aacctgggct caaagcttcc ttatctatgc cgggccggcg
      781 gattacgacc tgatgaaaag ctacgaggac accaagctgg agctgatccc ggaccgcggt
      841 cccggctggt tgcgctggct ggccaacgcc atcgcctggc tgctaaaatt cctgcacagc
      901 ttcatcccca actatggcat cgttatcatc attttctcct tcatcctcaa gctgattttg
      961 caccccctca ccaagaaaag catggacgcc aacctgaaaa tgcagagcat ccagccccaa
     1021 ttgcaggaaa tccaaaccaa atacaagaac gacccgcaga ggatgcagca ggaaatcgcc
     1081 aaagcataca aggaggccgg caccagtcca atgtcgggct gcctgccgat actgctgcag
     1141 atgccgattt tcatcgctct ttacaacgtt ctgcgctacg ccatggcaat gcgcaacgtg
     1201 agttttctgg ggctgaatct ctccgagccg gacccctgga tcctgcccgc catcatggcc
     1261 ggattcatga ttttgcaaag cctgatgacc cggccctcca aggaagccat ggccaagatg
     1321 gacgagaaac agcaggccat gcagcagagt tccaggatga tgacctggat catgcctgtg
     1381 atgctgttct tcatcttccg cggcctcccc tccggcctgg tgctttacta cactgttttc
     1441 aacatgctca gcgtggccca tcagtattac acgcaaaaac gtcttaaaca aaaggaaact
     1501 atttcatgac ctcaatcgag aaaaccggnn nnnnnnnnca gcatcgaagc aatcatcgcc
     1561 gccttccgcg gcgaacaccg gatcagggac tgggaactga actacaagat tgtgaaaaaa
     1621 ccctccaaag gtctgtttgg cctgtttggc agaaagcaag ccgtggtgcg tttcgagctg
     1681 cccagcctgg gcgaacgcgc cggcaacctg ctcagccagc tgctgggcaa gatgggcatc
     1741 cctttcagcc gcgttaccac caaaaccgaa ggcaaatcca tccatctgga gatcaaagac
     1801 agccaggaca caggactgct catcggcaaa aacggctata tgctggaaac cctgcaatat
     1861 tttgtgaacc gcgttttcga aaacgaacac gatatcgacc gcatctacat tgacgtggac
     1921 ggctaccgcg aacgccgcga agcccagttc ctgaagaaat tccaacccat catgaagaag
     1981 gtgcgcgaac agggcaaacc ccaaaccctg gaacccatga gctcaaccga ccggaggata
     2041 atccaccgtt acgtggagcg cgaacgcggc ctccgcaccc tcaccatcgg cgagggcgaa
     2101 cagaaacgca tcgtcatctt ttccgccagg caaaaggaga gtgaggcgct ctcccaggcc
     2161 caggacagca aggacaaaaa gccccgcagc ctcaggcgcg aacgcggcgg acgccccaaa
     2221 ctcaacacca gaccggagca cagcctgaaa cgggaacaga acctgaagcc ggagcaaaat
     2281 cccaaacccg ggcaaaggcc ccgccccaac cgccgtcccc ggcccaaggg caaaacctcc
     2341 gccaaggcat gaaaccggat atcagcctgc acaaaatcga cgcgggaaat tactggaccg
     2401 acggcggcgc catgctgggc gttttgcccc gcgccatctg gggaaaaagc gtccagaccg
     2461 acgagcggca ccgtaaaaac ctggctctga acctgctgct gatcaaaatg gcggaccgcg
     2521 ttatcctggt ggatacaggg cttggcaatc ggctttcgga aaagcagcgc gacatctacc
     2581 gcccctcggc cttcctgctg ccggtgtccc tggctgaact gggaatccgc gatgccgacg
     2641 tcaccgacgt ggtgctcacc catctgcatt ttgaccacgc cggaggcatc attacaggtt
     2701 ttggcgacca cgacgccctc acctttccca aagcccggca ctggatccag tgccaggaat
     2761 gggaaaccgc caaaaatccc gacggcctca accgcgcgtc ttacgatttt ccgcggcaac
     2821 tggccctgct ggaagagcgg ggaaggcagc aactcatcga tggcagcgag gaaatcgcgc
     2881 caggagtgac cctggtgaaa accggcgggc acacctgcgg cagccagatt gtcgagatca
     2941 attccgcgca tggatacttc atctacgccg gggacatcat gcccaccctg ttccacgttt
     3001 cgccggccgt cacctccgcc tacgacgttt gccgcgagga taccttcaag gccaagcagt
     3061 atatttccgc ccggctcaaa gaaaaaaacg gcaccctgct gctgaaccac gacccacggc
     3121 actgggaatt gccggcctcc gccctgaaat aataagttca gtcccccgcc cggctccagt
     3181 gtccgccctg gggatgcggt ctggccgtta aggccttgtc ccgtgtttgg gataagcctc
     3241 ctgtataatg cccgcattcg gtgcggggat tatgagggtg gcgtaagagg gggatggact
     3301 cgggcgttaa gggtgtggct gggcaggata gcacgataga taagcatttt ctggcgctct
     3361 ttacgctctt tttccgaagg ccggtccggg atgggtttcc cgcgcgcaag gcagagattt
     3421 ctgttgccat aatcccgcgc ctgaaaatct tggtttttaa tataatcaaa gaaaaaggag
     3481 gttgccatga tccagtattt caaggtcaga gagcagcgtt tcgagccggc tctggcgcac
     3541 agcgaggcgt tttggataca tctggaaagc cccacggcgg acgagatcaa ggcgctgatc
     3601 gaagaattcg atttgcccga agacttcatc acggacctcc aggacgcgga tgaaaccagc
     3661 cgcatggaat atgaggataa cgccgtcctc gtgatcatgc gcgtgccgct ctattacagg
     3721 caccgttcgg ccagtttagc attcactacg gcgcctttgg gcgtgatcgc ggtgcaggat
     3781 aagctcatca cagtgtcttt ctttgacaac gaagtgctca acgtctttct ggactgcaag
     3841 cacaagcctt tccggatcac ccagcagagt ttcctgctgc agatcgccct gcgcatctcg
     3901 ctctatttcc tgcgcttcct gaaagagata aaccgccgca ccaacaagat cgaaagcgaa
     3961 ctaagccaaa gcctgcgcaa ccaggagctc ttccgcctgc tgcggctgga aaagagcctg
     4021 gtctatttct caacttcgct aaaatcgaac gagatcatcc tggaaaggct gcagcgttcg
     4081 cgctggctga atcaggaccc ggaatccgag gacctggtgg aggacgtgat catcgacaac
     4141 acacaggcca tcgagatggc gaacatccac agcagcatcc tgagcggaat gatggatgcc
     4201 ttcgcctcca tcatttccaa caacctgaac gtggtgatga agttcctcac ctccgtcacc
     4261 atcatccttt ccgttcccac gctcattgcc agcctctacg gcatgaacgt gagactgcct
     4321 ctggagggga atccccacgc cttttgggcg gtgatcggga tttctgtgct ggcttcgctg
     4381 gtgctggtct tgatcttcat ccgcaaaaag tatttttaag ggaccgatat gtataaactt
     4441 agcgttaccg attccttcag cgccgcccac cgcctctgcg gctatgaagg cgcctgcagc
     4501 aacctgcacg ggcacaactg gaccgtgcgg gtggccctgg aagccgagcg gctcgacaat
     4561 atcggcatgg ccatggattt cggccatatc aaggctctgc tcaaagaaat caccgacggg
     4621 ctggaccacg cctatctgaa tgacctcccg gccttctcag aacagaaccc cacctccgaa
     4681 aacctcgccc gccacatctt tgaacgcatg gcggagacgc tggccggaaa accggtccgg
     4741 gtcgccgagg tggaggtgcg cgaatcagag cgttccagcg tgacttatag ccatgcttag
     4801 gtttgttgaa tcctttttcg taaagagcgc ggtggagccg aaggattatc ccgccagtgc
     4861 ctatcctgaa ttcgctgtgg cgggacgcag caacgtgggc aaatccaccc tcatcaacct
     4921 gctcacggaa aggcgcaaac tggccaaaac cagttcccac cccggcaaga cccgcctgct
     4981 caactttttc ctcatccgct ggaaggacga tgacgccgga aaagagggct gtttgcagct
     5041 taccgacctt cccggctacg gcttcgcgga agtgagcca
//
LOCUS       DAOOWR010000100         5063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16671_length_5063_cov_3.881589, whole genome shotgun sequence.
ACCESSION   DAOOWR010000100 DAOOWR010000000
VERSION     DAOOWR010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16671_length_5063_cov_3.881589"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /locus_tag="PLB85_03510"
     CDS             <1..2120
                     /locus_tag="PLB85_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU72426.1"
                     /translation="ELVDGKTPADNHGGIYILKLSVEPGRHRYKLIVDGIWMPDPANP
                     LREPDPFGGENSLLVVDPEGPSRLSWNQLREDLSLLHSRRDRYLELNRISEGDFELRF
                     IWHPGLEADMTAVIEGVEYKLHPLGVSGKNAVWHCFFQTSLPKVGMLIRVETDGLSLG
                     YGAEGFSFDPDSLSPRQLETASLPVFAVPDWVRSAVIYQIFPDRFCNGDPGNDPDFSE
                     DYYTDCRTPPPEGELLPPQREYFHLVRDWDNISGLKQSPWLPEGKPDWWSFYGGDLRG
                     VRQKLPYLKELGINVIYFNPLWQARSNHKYDAADFKRVDPHFGTGSELLDLVKAAHEE
                     GIRVILDVAFNHTGEDFWAFRDCVEKGPDSDYWNWYDWHKWPLPEPLPPDFQPKEYYQ
                     SWWGIKDMPDLNFDLSRAHPAENYVKDINQAVPNQPLVEHLLDCARWWLLEMGMDGFR
                     LDVPNEVPYWFWELFRRHVKELKPEAWIVGEIWQSARGWVNHRYFDSVMNYAHFKDPV
                     IEFFILGITDRKSFCDRVSAGLAEYPAQAAGAMMNLLGSHDTVRILELAGGDIERLKR
                     ALLFQMTFVGAPHIYYGDEIAMAGGRDPDNRRPFNWNWERNNKAVELRGFYQELIRLR
                     KSCQLFTEGEFAFLEVPDGMLAWERYDSSASVKVVLNLSTVTRKYRPEHGAERIFSLG
                     GLGEEGSVWHLKPGAAVVWHLEH"
     gene            2202..3377
                     /gene="coaBC"
                     /locus_tag="PLB85_03515"
     CDS             2202..3377
                     /gene="coaBC"
                     /locus_tag="PLB85_03515"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOU72427.1"
                     /translation="MDKNILLGVSGGIAAYKAIDLASKLSKAGYSVKTILTENASRFV
                     AGINFAAITHGSVHSQLFEDADPIPHITLADWADLIVVAPATANILAKAAQGIADDLL
                     STTLLAHAEPVLYVPAMNVNMYQSQATQANLNILRVRGNHVLEPNTGLLACGYEGKGK
                     YPPNAEVANAIRCYLEYPEDLDGISVLVTAGATAEPIDPMRLITNKSSGRMGLAIARA
                     FALRGAKVTLVHGLMEETPPYYLHQAIFAPTVQLMYDAVIKAAKGCRIIVKCAAVSDF
                     KPLKASSHKIKKGGSLNLELAPTPDILAQLGKQKTKKQKLVGFAAETDNLIANAREKL
                     QKKNLDLICVNHLDTAGADQTTLSVISAGDTKNKKPLQLSGDKFEVALRLVERIVKP"
     gene            3374..4153
                     /locus_tag="PLB85_03520"
     CDS             3374..4153
                     /locus_tag="PLB85_03520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU72428.1"
                     /translation="MKQLLIITGANGQVGSYLAQAWSETRLPMLLLIHSDSWRLEKLS
                     SRPEIMIKACDLRDLASFKAVLDEACRALDTVPARLVHTAAIRSYDARTLAEGEPLIW
                     SEVFNANVSMAYNVLKACLPGMLSGRFGRIVMFASNVVQTGLHSGSAYAAAKTAIVNL
                     VRSVALEAAPDNVLINSISPAPVETDLESDYEGEYLAFRQRYFAAYRAASPTGKLVSM
                     QELKRVAELLLSEELANLTGQNLVIDGGASAPLPASPETGN"
     gene            4189..>5063
                     /gene="queA"
                     /locus_tag="PLB85_03525"
     CDS             4189..>5063
                     /gene="queA"
                     /locus_tag="PLB85_03525"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOU72429.1"
                     /translation="MPDLLDVESYNYELPPELIAQHPLARRESSRLMCVERSTGNIVH
                     RRFSDVLGLLAPGEVLVLNSSKVIPARLFGEKSTGTRIEVLLLHKLDEARWKCMVHPG
                     KRLKTEQWLRFSPTLKGWLSLADEDGLREIEFGEPDTYWQEIERIGHVPLPPYIKRPD
                     NSSDRGDYQTVYAKSPGSVAAPTAGLHFSSELLEALKQKGVRVVEVILHVGMGTFLPV
                     KSQRVDAHKMHSEFCTMPEETAAEINSAKAEGRRVIAVGSTSVRTLESFWNGKSLKSG
                     SLWTDIFIYPGREIKV"
BASE COUNT         1123 a         1435 c         1459 g         1046 t
ORIGIN      
        1 gcgagctggt ggacggcaag actccggccg acaaccacgg cggcatctac atcctcaaat
       61 tatcggtgga gccgggacgc caccgctaca aactgatagt ggacggaatc tggatgccgg
      121 acccggccaa tcctttgcga gagccggacc cctttggagg cgagaattcc ctgctggtag
      181 ttgatcctga ggggccatca cgccttagct ggaaccagtt gcgggaggac ctttccctgc
      241 tgcactcacg ccgggaccgc tatctggagc taaaccgcat ttcggaaggg gattttgagc
      301 tgcgcttcat ctggcatccc gggctggaag ctgatatgac ggcagttatt gagggcgtgg
      361 aatacaaact ccatcccctg ggtgttagcg gcaaaaatgc agtctggcac tgctttttcc
      421 aaacttccct tcccaaggtg gggatgctga tccgggttga aacagacggg ctttccctcg
      481 gctacggcgc ggaaggattt tccttcgatc cggacagcct ttcacccagg cagttagaga
      541 ctgccagcct gccggtgttc gcggttccgg actgggtgcg ctccgccgtt atctaccaga
      601 tctttcccga ccgtttttgc aacggcgatc ccggcaacga tcccgacttc agcgaagatt
      661 actacacgga ttgccggacc ccgccaccgg aaggcgaatt gctgccgccg cagcgcgaat
      721 acttccatct ggtgcgggac tgggacaaca tcagcggcct gaagcaaagc ccctggcttc
      781 cggaaggaaa gccggattgg tggtctttct acggcggcga cctccgcgga gtgaggcaga
      841 aattgcccta tctgaaagaa ctgggcatca acgttatcta tttcaacccg ctctggcagg
      901 ccaggtctaa ccacaaatac gatgccgccg atttcaagag ggtcgatcct catttcggca
      961 cgggctctga gctgctggac ttggtgaaag ccgcccatga agaaggaatc agggtgattc
     1021 tggatgttgc tttcaatcac accggagagg atttttgggc ctttcgcgac tgcgtggaaa
     1081 agggcccgga ttcagactac tggaattggt atgactggca caaatggcca ttgccggagc
     1141 cgttgccgcc ggatttccag cccaaggagt attaccagag ctggtggggc atcaaggaca
     1201 tgccggacct caattttgac ctcagccgcg cccatcctgc cgagaactat gtgaaagaca
     1261 tcaaccaggc cgttcccaat cagccgctgg tggagcatct gctggattgc gcgcgttggt
     1321 ggcttctgga aatggggatg gacggcttcc gcctggatgt gccgaatgaa gttccctact
     1381 ggttttggga gcttttccgc cgccacgtga aagagctgaa gccggaggcc tggatcgttg
     1441 gcgagatctg gcaaagcgcc cggggctggg tgaatcaccg ctatttcgac tccgtgatga
     1501 actatgccca tttcaaggac cccgtcattg agtttttcat cctgggcatc acagaccgca
     1561 aaagcttttg tgaccgcgtc tcagcggggc tggcagaata ccctgcccag gccgctggcg
     1621 cgatgatgaa cctgcttggc agccacgaca ccgtgcgaat ccttgaattg gccggaggcg
     1681 acattgaacg cctgaaacgg gctttgcttt tccagatgac cttcgtgggc gctccccaca
     1741 tctattacgg tgacgaaata gccatggcag gaggccggga tccggacaac cgcaggccgt
     1801 tcaactggaa ctgggagcgg aataataagg ctgttgaact gcggggattc tatcaggaac
     1861 ttatccgcct gcgcaaatcc tgccagcttt tcacggaggg ggaattcgcg tttttggaag
     1921 ttcctgacgg catgctggcc tgggaacgat acgattcatc ggcaagcgta aaagtggtgc
     1981 tgaacctttc taccgtgacc cggaagtatc gcccggagca tggcgcggag cgcattttca
     2041 gcttgggcgg gcttggagag gagggttcgg tctggcatct gaagcccgga gcagccgtgg
     2101 tgtggcacct ggagcattga agtggggcag aggccgatgt tttggattga cacaatgcgg
     2161 agaggacaaa attatggcgt caaacatcta aagagcggtt catggacaaa aacatactac
     2221 ttggcgtgag cggcggcatc gccgcttaca aagctatcga cctggccagc aagctgagca
     2281 aggcgggtta cagcgtgaag accatcctga ctgaaaacgc ctcgcgcttt gtggcgggga
     2341 tcaacttcgc cgccatcacc cacggcagcg tgcacagcca gcttttcgag gacgccgatc
     2401 ccattccgca tatcactttg gcggactggg cggacctgat cgtggtggct cccgccacag
     2461 ccaacatcct tgccaaagcg gcccagggca ttgccgacga tctgctttcc accactctgc
     2521 tggctcatgc cgagcctgtt ctttacgttc cggcgatgaa tgtgaacatg tatcagagtc
     2581 aggccacaca ggccaacctt aacatcctgc gagtgcgggg aaaccatgtt ctggagccga
     2641 atacagggct gctcgcctgc ggctatgagg gcaagggaaa gtatcctccc aacgcggagg
     2701 tcgcaaatgc catccgctgc tatttggaat atcctgaaga ccttgacggg atcagcgttc
     2761 tggtcaccgc aggcgccaca gcagagccga tagatcccat gcgcctgatc accaacaaat
     2821 ccagcggcag gatgggcctg gccatcgcca gggctttcgc cctcaggggc gccaaggtca
     2881 ctctggtcca cggtttgatg gaagaaaccc ctccctacta tctgcatcag gccatcttcg
     2941 cacccacggt gcaactgatg tatgacgccg ttatcaaggc tgccaaaggc tgccggataa
     3001 tcgtgaaatg tgccgccgta tctgatttca agccgctcaa agcatcttcc cacaagatca
     3061 agaaaggcgg cagcctcaac ctggaactgg cacccacgcc ggatatcctt gcccaactcg
     3121 gaaaacagaa aaccaagaaa cagaagctgg tgggctttgc cgcggagacc gacaatctga
     3181 tcgccaacgc cagggaaaag cttcagaaaa agaacctgga cctcatctgc gtgaatcatc
     3241 tggacaccgc gggggctgac cagaccacgc tttctgtcat cagtgccggg gacaccaaaa
     3301 acaaaaaacc cctgcaactg agcggcgaca agtttgaagt cgcactgcgt cttgtggagc
     3361 ggatcgtaaa gccatgaagc aattgctgat catcaccggc gccaacggcc aggtggggtc
     3421 ttacctcgcc caggcatggt cggaaacgcg tttgccgatg ctcttgttga tacattctga
     3481 tagctggcgt ttggaaaagc tgagttcccg gccggagata atgatcaaag cctgcgactt
     3541 gcgcgacctt gcctctttca aagccgtcct ggatgaagct tgccgggcct tggacaccgt
     3601 tccggccagg cttgtgcaca cagccgcgat acgcagctac gacgccagaa ctctggctga
     3661 gggcgagccc cttatttgga gtgaagtttt taacgccaac gtgagcatgg cctataatgt
     3721 cctcaaggct tgtctgccgg gtatgttgag tggtagattt ggccggatcg tgatgttcgc
     3781 ttcgaacgtg gtccaaaccg gcctgcacag cggctctgcc tacgcggcag cgaaaaccgc
     3841 gatcgtgaat ctggtccgca gcgtggcttt ggaggctgcc ccggacaacg ttttgatcaa
     3901 ctccatttct ccggccccgg tggaaactga ccttgaaagc gactacgagg gtgaatacct
     3961 ggctttccgc caacgctatt tcgctgcgta ccgggcggcc agccccaccg gaaaactcgt
     4021 ctccatgcag gaactgaagc gcgtggcgga actgctgttg agtgaagaac tggccaacct
     4081 caccggccag aacctggtga tcgacggagg cgcctcagcg cccctgcccg cttcgcccga
     4141 aaccggcaat tgaatccaaa tccaatcccc cgccaagact ttcaggccat gccggacctt
     4201 cttgacgtcg aaagctacaa ctatgaattg ccgccggagc ttatcgccca gcatcccctt
     4261 gcgcggcgtg aaagttcccg cctgatgtgt gtggaaaggt ccacgggaaa cattgtccac
     4321 cgcaggtttt cggatgtttt gggtctcctc gctcccggtg aggtgctggt tttgaattcc
     4381 agcaaggtga ttcccgcccg cctcttcggg gaaaaatcca ccggcaccag gatagaggtg
     4441 ctacttttgc acaagctgga tgaagcgcgc tggaaatgca tggtccatcc cggcaaaagg
     4501 ctcaaaaccg agcagtggct gcgattctca ccaaccttga aaggctggct gtctctggct
     4561 gacgaagacg gattgcggga aatcgaattc ggggagccgg atacttactg gcaggaaata
     4621 gaaaggattg gacacgttcc tctgccgcct tacatcaagc gtccggacaa ttcatccgac
     4681 cgcggtgact atcaaaccgt ctatgctaaa tctcctggct ccgtggctgc tcccaccgcc
     4741 ggacttcact tcagctctga actgctggaa gcgctgaagc aaaaaggggt gcgggtggtg
     4801 gaggtgatcc tccatgtggg gatgggaacc tttttgccgg tgaaaagcca gcgcgttgac
     4861 gcccacaaga tgcacagcga gttttgcacc atgccggagg aaacggccgc ggaaatcaac
     4921 tccgccaaag ctgaagggcg gcgcgttatc gccgtgggca gcacttccgt gcgcaccctg
     4981 gaaagtttct ggaatggcaa aagcctcaaa agcggctcgc tatggactga cattttcatc
     5041 tatcccggcc gtgagatcaa ggt
//
LOCUS       DAOOWR010000101         5053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16718_length_5053_cov_3.882753, whole genome shotgun sequence.
ACCESSION   DAOOWR010000101 DAOOWR010000000
VERSION     DAOOWR010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16718_length_5053_cov_3.882753"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLB85_03530"
     CDS             complement(<1..1292)
                     /locus_tag="PLB85_03530"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU72430.1"
                     /translation="MHNFNIVKRELDFCGRPLYVETGRMAKQANGSVFIRYGGTAVLV
                     TATMSKEPAENQDFFPLTVDYIEKMYASGKIPGGFFKREARPTTDATLSARVIDRSIR
                     PLFPEGFRNQVHVVLNVLAYDGENDASVLGVFGASLALTLSDIPFHGPIAGVKVGHID
                     GQIVVNPYKSQMDENSRLDLDVAASATSVVMVESSALELSEETILDAVYTGHEEIKKL
                     CALQLDMAAEIGKEKVEVTLLEIPAEIMGKVEAGYGSRIAEAAVIFGKQERQDAFDAA
                     EAELLEKALEEDPEGFEENEKWYKAAFEELIRRYVRESILEKHHRVDGRGLDDIRDIT
                     CEIDVLPIVHGSALFTRGETQSLGTVTLGGGSDEQVIDGLEVEYKKHFYLHYNFPPFS
                     VGEAGRMGPVGRRELGHGNLAERALKAVLPDREAFPYT"
     gene            complement(1486..1755)
                     /gene="rpsO"
                     /locus_tag="PLB85_03535"
     CDS             complement(1486..1755)
                     /gene="rpsO"
                     /locus_tag="PLB85_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU72431.1"
                     /translation="MALKPEAKTAIMAEFKLHESDTGSPEVQVALLTRRINDITTHLK
                     EHSKDFSTRRGLLKLIGQRRRLLDYLMRKDINRYRELIKALKIRR"
     gene            complement(2475..4079)
                     /locus_tag="PLB85_03540"
     CDS             complement(2475..4079)
                     /locus_tag="PLB85_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72432.1"
                     /translation="MKKWIVIVLAAMLGLYACAGTKTVTEAVPKAEITTKVAILPLKA
                     LDSSSRYITKILTVRDLDLTFDKYANYVLLDMDETAAHFRETGYRDVEDLEKEEMVEI
                     SKNLASDVILIGNVSESRGLYNITMRMYSTLSGELKQISFNVGKEKTSRWQALDESMM
                     KELDGFISGEMDKIFNIATSYYNNGNYVEAEKSLKQVVALKPDKVEAYIFLGNTYLKT
                     DRIDLAEATFVKGYEIDSKNLTIMRALNDIYEKTNQTAKRIALMEGYAEASNDAETWL
                     AVGNLYDQQGNSAKAKASFQKALAIDPEYSAANVRLAFMLYDEGSYAESIPLLEEAFE
                     LAPDNDLVSSRLATAYQKSGRIQDAIAKYEGLIKNDPNNVNAYLNLVNLYRTDNKDSK
                     AIETVNALKKVDPNNPYVYLNLAAIYLSQNKYNDAETNANLTLSKDSSLYQPYVILAS
                     VFQSRGTDAYNTYLDLDRQASAAVGKKATDLRKKRDAAKGNAQSLLGKSRDHLNSARN
                     RASESSAINDINNRLGRVNELIGKLQ"
     gene            complement(4170..4877)
                     /locus_tag="PLB85_03545"
     CDS             complement(4170..4877)
                     /locus_tag="PLB85_03545"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU72433.1"
                     /translation="MITRLLYDTLDNSILEYDRLASIGSASDRWIVRAKSQTAGVGRN
                     ENTWHSPRGGLWLSFDFHYPRAVPSFPLYVGYCLHNVLSRSFGLDKLSVKWPNDLYLE
                     DKKLAGILCQHRQNPSRYLVSLGLNSNCEMDDVLLELNAANLSSYMKMPISNSCLASL
                     VVQSIRNHSAMLATPEVYLDYCGKHLYGLGREAELESGGSMLRGRISGLSDEGFLLLR
                     DNEGRIETITHGSLRII"
     gene            complement(4874..>5053)
                     /locus_tag="PLB85_03550"
     CDS             complement(4874..>5053)
                     /locus_tag="PLB85_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOU72434.1"
                     /translation="PGREIKVADALITNFHLPKSTLLMMVSAFAGHELVRRAYQLAVD
                     EKYRFFSYGDAMYIS"
BASE COUNT         1051 a         1273 c         1428 g         1301 t
ORIGIN      
        1 ttcgtgtatg gaaaagcctc gcggtccggc aaaacggctt tcagggcgcg ttcagccagg
       61 tttccgtgcc ccagttcgcg gcgtccaacg ggacccattc tgccagcttc gcccacgctg
      121 aagggcggga agttgtaatg caggtaaaag tgtttcttat actcgacctc gaggccatcg
      181 ataacctgtt cgtcgctgcc gccgccgagg gtgacggtgc ccagggactg ggtttcgccc
      241 ctggtgaaca gcgcggagcc atgcacgata ggcagaacgt cgatttcgca ggtgatgtcg
      301 cggatgtcat ccaggccgcg gccatcaacg cggtggtgtt tttcaagaat ggattcacgc
      361 acgtagcggc ggatcagctc ttcaaatgca gctttgtacc atttttcatt ctcttcgaag
      421 ccttcgggat cctcttccag agccttttcg agcaactccg cttcagcggc gtcaaaggcg
      481 tcctggcgtt cctgtttgcc aaagatgacg gcggcttcgg cgatgcggct tccgtagccg
      541 gcttccactt tgcccatgat ctcggccggt atctccagca aagtcacttc cactttttct
      601 ttgccgattt cggcggccat atccagttgc agggcgcaga gctttttaat ctcttcgtgc
      661 ccggtgtaaa ctgcatccag gatggtctct tcactcagtt ccagggctga ggattccacc
      721 atcaccaccg aggtggctga agcggccacg tccaggtcca gcctggaatt ttcgtccatc
      781 tggctcttgt aggggttcac aacaatctgc ccgtcgatgt ggccgacttt cacgcccgcg
      841 atggggccgt ggaaaggaat gtcggaaagg gtgagggcca aagaagcgcc gaaaacgcca
      901 aggacagagg cgtcgttctc cccgtcgtaa gcgaggacgt tcagaacaac gtgcacttgg
      961 ttgcggaatc cctcaggaaa caacggcctg atgctgcggt caatgaccct ggcagaaagg
     1021 gttgcgtcgg ttgtgggacg ggcttcgcgt ttgaaaaagc cgccggggat cttgccgctg
     1081 gcgtacattt tttcgatgta atcaaccgtg agcgggaaga aatcctggtt ttcagcaggc
     1141 tctttgctca tggtggcggt aacgaggaca gcggtgcccc cgtaacggat gaaaacgctt
     1201 ccgttggctt gtttggccat tctgccggtt tccacgtaga gcggacgtcc gcagaaatcg
     1261 agttcgcgtt tgacaatgtt gaagttatgc atatatttct cctggttgtt ccgagggaga
     1321 agaagcaata ggctgaattc ccggagtggc tttcagctta ttgctcgtac tcccctggag
     1381 gttgaagtct cgtgtccgga accgttccgg acgtttagat aagagaaagg tggatcccgc
     1441 caaaacaatt tagtgagaat ccacctcgtc ctattttggc ggaatttatc tgcggatttt
     1501 aagcgctttg atcagctcgc ggtagcggtt gatgtctttc cgcatgaggt agtcaagcaa
     1561 gcgccttctc tgcccgatca gtttcaacag cccgcgtctg gtgctgaagt ctttggaatg
     1621 ctctttcagg tgggtcgtta tgtcattgat ccgccgggtg agaagagcaa cctgaacttc
     1681 aggcgagccg gtgtctgatt catggagttt aaactctgcc atgatagccg ttttggcctc
     1741 aggtttaagt gccatttcat tctcctatgg aattttgtgt cagctttttg gatgcaggcg
     1801 atttgtccag ttcttttttc cgttcccttg caaatataat gtttacccat cacgatatcc
     1861 tgacggtcag acccttagcg cttgattcca tcccgctccc ccgcctcccg gtaaatcctc
     1921 gcatcggatg cgggtatcgg gcgggaggcg aaactgaaac acagtatagg gccttaacgg
     1981 cctgaggata tcgattatcc cgaccacggg ctgtcgtcga gcgaaggatg agcttcgacg
     2041 gcagcaatga atgaaccttc cgatacttcc tgtcgtccat gctccctgcg ttcgctgtac
     2101 gccaggatac aggatacgtc atcccggacg tcaccgagct tgcgaggtga cattgatccg
     2161 ggatccacga agggatatgc aatggattcc gggtcaagcc cggaatgacg tgggtttgag
     2221 actgggtggg ggtttatggg ctattccgcc ccggtctgga ttccgggtca agcccggaat
     2281 gacgtggtgg gctggcgtcg agcgaggaac gagcatcgac gacagtaatg agttatcctt
     2341 ttgatacttc ctttcatcca tgccgactgc gttcgctgta ggccaagggc aacatcgaaa
     2401 tagggacttt acccgtccat aaaaaaagaa cgcccggaaa acccgggcgt ctttacaatc
     2461 ataactatgt atatttattg caacttgccg atgagttcgt taacccggcc gagccggttg
     2521 ttgatgtcgt ttatagcgga cgattcgctg gctctgtttc tggcgctgtt caggtgatcc
     2581 cttgatttgc caagcaacga ttgggcgttg cctttggccg cgtcgcgctt ctttctgagg
     2641 tcggtggctt ttttgccgac cgcggctgag gcctggcggt ccaaatccag ataggtgttg
     2701 taggcgtcgg tgccacgaga ctggaagacc gaagccagga tcacataagg ctgatacagg
     2761 ctggagtctt tggaaagggt gaggttagcg ttggtttccg cgtcgttgta tttattctgg
     2821 gagaggtaga tggcggctag gttcaggtaa acgtaaggat tgttggggtc aaccttcttg
     2881 agggcattga cggtttcgat cgccttggaa tccttgttgt ccgtgcggta gagattcacc
     2941 aggttcagat aggcgttcac gttgttggga tcgtttttga tcaggccttc gtatttggcg
     3001 atggcgtcct ggatgcgtcc gctcttctga taggcagttg ccaggcggct gctgacgaga
     3061 tcgttgtccg gggccagttc aaaggcttct tcgaggaggg gaatcgattc ggcatagcta
     3121 ccctcatcgt aaagcatgaa agccaggcga acgttggccg ccgaatattc cggatcgatg
     3181 gcaagggctt tctggaatga ggctttggcc ttggcggaat tgccctgctg gtcatacagg
     3241 ttgcccacgg ccagccaggt ttcggcatcg ttggaagctt ctgcgtaacc ttccatcagg
     3301 gcgatgcgct tggcggtctg gtttgttttt tcatagatgt cattcaacgc gcgcatgatg
     3361 gtgaggttct tggagtcgat ttcatagcct ttcacgaagg tggcttctgc caggtcgata
     3421 cggtcggttt taaggtaggt gttgccaagg aagatatagg cttccacctt gtcaggcttc
     3481 aacgccacga cctgcttgag cgatttttcg gcttccacgt agttgccgtt gttgtaatag
     3541 ctggtggcga tgttgaagat cttgtccatc tcgccggaga tgaacccgtc gagttctttc
     3601 atcatgcttt cgtccaaagc ctgccagcgg gaggtttttt ccttgccaac gttgaagctg
     3661 atctgtttga gttctccgga aagggtgctg tacatacgca ttgtgatgtt ataaaggccg
     3721 cggctttcgc tcacattgcc tatcaggatc acatcggaag cgaggttttt ggatatttcc
     3781 accatctctt ccttctcgag gtcttccacg tccctgtatc cggtttcgcg gaagtgcgct
     3841 gccgtttcgt ccatgtccag aagaacgtaa ttggcatatt tgtcgaaagt gaggtcaaga
     3901 tctctcacag tgaggatctt ggtgatataa cggctggaac tgtccagcgc cttcaagggc
     3961 aggatcgcca ctttggtggt gatttcagcc ttgggcacgg cctcagtgac ggttttggtg
     4021 ccggcacagg catagaggcc aagcatcgcg gcgaggacga ttactatcca ttttttcatt
     4081 aattcctccc ggaaattata tttctcttct attgtcgcaa agcatttcca acggccttat
     4141 ctgtcaagaa aaagctgccg gataagccgt cagattattc tcaaactgcc gtgggtgatc
     4201 gtttcaatcc tgccttcatt gtctctcaaa agcaggaaac cttcgtctga aagcccagat
     4261 atcctccccc tcagcattga gccgccggat tcaagctccg cctcccgccc caagccatac
     4321 aggtgtttgc cgcaatagtc aagatatacc tccggcgtgg caagcatggc ggaatggttg
     4381 cgaatacttt gaaccaccaa gctggcaaga cagctgttgg atataggcat cttcatgtag
     4441 gaggatagat tggccgcatt cagttcaagt aatacatcat ccatttcgca gttgctgttc
     4501 aagcccaggc taaccaggta acgggaggga ttttgccgat gctggcagag gatcccggcc
     4561 agttttttat cctccagata gaggtcattg ggccacttca cgctaagctt atcaagcccg
     4621 aatgaccggg aaagcacgtt gtgaagacaa taacccacat agagcggaaa agaaggcaca
     4681 gcccggggat aatggaaatc aaaactgagc cagaggccgc cccggggaga atgccaggtg
     4741 ttttcgttgc gcccgacccc ggcggtttgg cttttggcgc ggacaatcca gcggtcggag
     4801 gctgaaccga tggaggccag cctgtcatat tccagaatgc tgttgtccaa agtatcgtat
     4861 aaaagccttg tgatcatgag atatacatcg cgtcgccgta gctgaagaag cggtattttt
     4921 cgtcaaccgc gagctgatat gcccggcgca ccaattcatg gcccgcaaag gcggaaacca
     4981 tcatcagcag ggtcgatttg ggcagatgga aattcgtgat gagggcatcg gcgaccttga
     5041 tctcacggcc ggg
//
LOCUS       DAOOWR010000102         5030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16807_length_5030_cov_3.165628, whole genome shotgun sequence.
ACCESSION   DAOOWR010000102 DAOOWR010000000
VERSION     DAOOWR010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16807_length_5030_cov_3.165628"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /gene="uvrC"
                     /locus_tag="PLB85_03555"
     CDS             complement(<1..1427)
                     /gene="uvrC"
                     /locus_tag="PLB85_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU72435.1"
                     /translation="MEQVPQKIAQKLHLLPQQPGIYIWKNAEGEVIYVGKARLLESRI
                     KSYLNNSPKDIKTEQLLRHIDDLDYIITLSEHDAFILEANLIRRHQPKYNILLKDDKR
                     YPFVRITLAEPFPRIMVTRELLKDGSRYFGPFTDAKSLRMTLRNFEWIFPIRTCSRVI
                     PADKVRFRQACINHQLGKCPAPCIGAVSQADYMRVVMRQMSFFEGKHQEVLDELRAEM
                     NGLSEELKFEQAAKTRDRILAIERIQKRQVVYSPDARNTDVIGFYQEDKIAVCVVLRI
                     MSGMVVNREDYPLGNTENDTPEEALAAFIKLYYSRRDELPDEILLPLEPAEFEELNSW
                     LGGKLSLPQRGEKSKLLAMAKRNAFQLIEERKLAHIRRANRTVFPIQELKEKLALPKL
                     PRKMVCMDISTIQGTDTVSSAVFFENGKAKKKFYRHFIIRDVDTQNDYAALQETLTRF
                     LGETARDESLLPDLIIIDGGKGQLG"
     gene            complement(1403..>1801)
                     /locus_tag="PLB85_03560"
     CDS             complement(1403..>1801)
                     /locus_tag="PLB85_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOU72436.1"
                     /translation="VKISASMVGLNIVLNFILMQFMQHRGLAFATSITAMVNFSIQFY
                     LLRKKMPHISFAGILPNFLKTLGICVLMLVLLLVANHFYHPRGFWPQLIKLGLLGTAS
                     LLFFYGLGLLLKLDFLAEARDRLWNKFRRK"
     assembly_gap    1802..1811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1812..2971)
                     /gene="murJ"
                     /locus_tag="PLB85_03565"
     CDS             complement(<1812..2971)
                     /gene="murJ"
                     /locus_tag="PLB85_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOU72437.1"
                     /translation="MAENKLIRNISMMSVAILISRVLGLVRDQLMAFFFGTTFLNDAF
                     VVGYKIPNLLRNLFGEGALSASFVPIYNEIGIKEDRRKQIDFALQILGILSVFLLTLT
                     VIGIIFAPWIVKLLYPGLAPHTYKLAVLLTRVMFPYLLWIGLSSTMIAILNSHDRFFM
                     TGLSSALLNVGMILSMVVPRFVFGLQGEQLVRWAAWGVFGGGFLQTVINFPYLKQLGY
                     PFKLFVRFGGEALNTMWKRFLPSMIGVGVREINIVADSLMASFLAVGSISALEYGYRL
                     FQFPLGMFAVSTGTVLLPTYSRLVTMGNWEELSKNLRVAAVNLIYLMLPITTLIIALG
                     GDLVQVVFQRGAFDAKASLWTEQALIFYSLGLIFFSFNQTLTPLFFANKDTRT"
     gene            complement(2974..4098)
                     /locus_tag="PLB85_03570"
     CDS             complement(2974..4098)
                     /locus_tag="PLB85_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOU72438.1"
                     /translation="MFRNIKGQAGALKILKLALENNRVAQAYLFHGSDGVGKFTTALY
                     FGMALNCLSKSEFRPCGVCASCRKFMALDHPDLIYLFPTPNLGLNLDGEITKSEFIPE
                     YAGYIRNKIETPWEDYQFSAKIMIRKESMSWLIKRLNLSSYEASHRICIIEDADMMNT
                     ETANAFLKTLEEPPESTVIILTTDNLAKLLPTIVSRCQPVHFQQVTPSVIEGILCEKF
                     SVSPEMARTASHIANGSVKRAIHFAHNESSELRERAFEILEMAASGKDLAYFRSLQGL
                     GKKFNAESAIEVLRYASLFAGDLNLVLDAPEQMVNIDKKEFLLGTREKLADVDPAAYT
                     DRVINFVLLMEDYVRKLHGNVNLQLVMLNLFVELKKLVRA"
     gene            complement(4130..4741)
                     /locus_tag="PLB85_03575"
     CDS             complement(4130..4741)
                     /locus_tag="PLB85_03575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOU72439.1"
                     /translation="MKYFRKLVGEKCYLSPISLDDVERYTEWVNDMEMGLFMQFSASI
                     FDLDKERELLQKIMEDSHSFAIVEKVTNKSIGNCSLMGVSEVHRHAKFGIFIGDKTYW
                     NRGIGTEATNLTLDYGFNVLNLRNITLDVYSFNKRAIRCYEKVGFKAAGLRRQFIFMA
                     GKYHDVLIYDILAEEFTSPYVSGLFERSTSEDAGRGKITFEKS"
BASE COUNT         1042 a         1279 c         1553 g         1146 t
ORIGIN      
        1 tcaccaagct ggcccttccc gccgtcgatg atgatcaggt cgggcagcag gctttcgtcc
       61 ctggctgttt cacccaggaa gcgggtgagg gtttcctgga gggcggcgta atcgttttgg
      121 gtgtccacgt cgcggatgat gaaatggcgg tagaatttct ttttggcctt gccgttctcg
      181 aagaaaacgg cggaggaaac ggtatccgtg ccctggatgg ttgaaatgtc catgcagacc
      241 atcttgcggg gtagttttgg aagggcgagt ttttccttca gttcctggat gggaaagacg
      301 gtgcggttgg ctctgcggat gtgggccagc ttgcgttcct cgatgagctg aaaggcgttg
      361 cgttttgcca ttgccagcag tttgctcttt tcgccgcgct ggggcagact cagtttccct
      421 cccagccagg aattcagttc ctcaaactca gccggttcca gcggcagcag gatttcgtcg
      481 ggcagttcat cccggcggga gtaatagagt ttgatgaagg cggccaaagc ctcttccgga
      541 gtgtcgtttt cagtattgcc gaggggatag tcctcccggt taacgaccat gccgctcatt
      601 atcctcagca ccacgcaaac ggcgatctta tcctcctggt aaaagccgat cacatcagtg
      661 ttccgggcat ccggggaata gaccacctgg cgcttctgga tgcgttcgat ggcgaggatg
      721 cggtcacgcg ttttggcggc ctgctcgaat ttcagttcct cggaaagccc gttcatctcg
      781 gcacgaagct catccagcac ttcctggtgc ttgccctcga agaagctcat ctggcgcatc
      841 accacgcgca tgtaatcggc ctgcgaaacc gctccgatgc agggggcggg gcatttcccc
      901 agttggtggt tgatgcaggc ttgcctgaaa cgcaccttgt cggccggaat cacccgcgaa
      961 caggtgcgga tggggaaaat ccactcgaaa ttgcgcaggg tcatccgcag ggatttggcg
     1021 tctgtgaatg gcccgaagta gcgggaaccg tctttcagca gttcgcgggt caccatgatg
     1081 cgggggaagg gctccgcgag ggtgatccta acgaagggat agcgcttgtc atctttgagc
     1141 aggatgttgt atttgggctg gtggcgccgg atcaggttgg cttcaaggat gaaggcatcg
     1201 tgttccgaga gcgtgatgat atagtccagg tcgtcgatgt ggcgcaggag ttgttcggtt
     1261 ttaatatctt tcggggagtt gttcaggtag cttttgatgc ggctttccag caagcgggcc
     1321 ttgcccacgt agataacctc tccctcggcg tttttccaga tatagatacc cggctgctgg
     1381 ggcaggaggt ggagcttttg ggctattttc tgcggaactt gttccatagg cggtcacgcg
     1441 cctcagccag gaaatccagc ttcagcagca gaccgaggcc gtagaagaag agcagtgaag
     1501 cagttcccag caggcccagc ttgatgagct ggggccagaa tccccgcgga tggtaaaagt
     1561 ggttggcaac gagcagcaaa accagcatca gcacgcagat ccccaaagtt ttcaaaaagt
     1621 tgggaaggat gccggcgaaa ctgatgtggg gcattttttt gcggaggagg taaaactgga
     1681 tgctgaaatt gaccatcgcg gtgatggaag tggcgaaggc cagaccacgg tgctgcatga
     1741 attgcatcag gatgaagttc agcacgatgt tcagccccac catcgaggcg gaaatcttta
     1801 cnnnnnnnnn ngtgcgggtg tccttgttgg cgaaaaagag cggggtgagg gtttggttaa
     1861 agctgaaaaa gatgaggccg aggctgtaga agatgagagc ctgctcggtc cagaggctgg
     1921 ctttggcgtc gaaggccccg cgctggaaga ccacctgcac gaggtcgccg cccaaggcta
     1981 tgatcagtgt ggtgatgggc agcatcaggt aaatcaggtt cactgccgcc acgcgcaggt
     2041 ttttggaaag ctcttcccag ttgcccatcg ttaccagcct ggaataggtt ggcagcagca
     2101 ccgtgccggt ggaaacggcg aacattccca gcggaaattg gaagagccgg tagccgtatt
     2161 ccagcgccga aatgctgccc acggccagaa acgaggccat gaggctgtcg gccactatgt
     2221 tgatttcgcg gacgccaact ccgatcatcg aaggcaggaa acgcttccac atcgtgttga
     2281 gggcttctcc gccgaaacgc acgaacagct tgaagggata gccaagctgc ttcaggtagg
     2341 ggaagttgat gacagtctgc aaaaaaccgc cgccaaacac gccccaggca gcccagcgga
     2401 ccagttgctc tccctgcagg ccaaagacga agcgggggac caccatgctg aggatcatgc
     2461 ccacgtttag cagagcggag gaaagccccg tcatgaaaaa gcggtcgtgg gagttgagga
     2521 tggcgatcat ggtggaggaa agcccgatcc agagcaggta gggaaacatc acgcgggtga
     2581 gcagcacggc cagtttgtag gtgtggggcg ccaggccggg atagagcagc ttcacgatcc
     2641 agggggcgaa gatgatgccg ataaccgtga gcgtgagcaa aaagacggag aggattccca
     2701 agatttgcag cgcgaaatct atctgcttcc ggcggtcctc cttgatcccg atttcgttgt
     2761 agatgggcac gaacgaggca gaaagcgccc cctcgccgaa gaggttgcgc agcaggttgg
     2821 gaatcttgta gcccacaacg aaggcgtcgt tgaggaaggt ggtgccaaag aagaaggcca
     2881 tcagttggtc gcgcaccagg cccaggaccc ggctgatgag gatggcgacg gacatcatgc
     2941 tgatgttgcg gatgagcttg ttttcagcca tgttcaggcc cgcacgagct ttttcagttc
     3001 cacgaagagg ttgagcatca ccagttggag gttcacgttg ccgtggagct tgcgcacgta
     3061 atcctccatc agcagcacga agttgattac cctgtccgta taggcggcgg gatcgacatc
     3121 cgccagcttt tcccgagtgc cgagcaggaa ttcctttttg tcgatgttca ccatctgctc
     3181 cggggcgtcc agcaccaggt tcaaatcgcc ggcgaagagg ctggcatagc gcagcacctc
     3241 gatggcgctt tcggcgttga atttcttccc gagcccctgc aaggagcgga agtaggccag
     3301 gtctttgccg gaagcggcca tttccaggat ttcaaaggcg cgttcgcgta gctcggaaga
     3361 ttcgttgtgg gcgaagtgga tggctcgctt cacgctgccg ttggcgatgt gtgaggcggt
     3421 gcgggccatt tccggcgaaa cgctgaattt ttcgcacagt atgccttcaa taacagaggg
     3481 ggtaacctgc tggaaatgca ccggctggca gcgcgaaacg atggtcggca gcagtttggc
     3541 cagattgtcc gtggtgagga ttatcacggt ggactcgggc ggctcttcca gggtcttgag
     3601 gaaggcgttg gcggtttcgg tgttcatcat gtccgcgtcc tcgatgatgc agatgcggtg
     3661 gctggcctcg taggatgaga ggtttagccg tttgatgagc cagctcatgc tttccttgcg
     3721 gatcatgatt ttcgcgctga actggtagtc ctcccagggc gtttctatct tgttgcggat
     3781 gtagcctgca tattcgggga tgaattcgct tttggtgatt tcgccgtcca ggttgaggcc
     3841 caggttggga gtggggaaga ggtagatcag gtccggatgg tccagcgcca tgaacttgcg
     3901 gcaggaagcg cacactccgc aggggcggaa ttcgcttttg gaaaggcaat tcagcgccat
     3961 gccgaagtag agcgcggtgg tgaattttcc caccccgtcg ctgccgtgga acaggtaggc
     4021 ctgggcgacg cggttgtttt ccagtgccag cttcaggatt ttcagggcgc ccgcctggcc
     4081 tttgatatta cggaacatct attctgccgc tactgccggt ttccctttct caggattttt
     4141 cgaaagtgat cttgccgcgg ccggcgtcct cgctggtgct gcgctcaaaa agaccgctca
     4201 cgtaggggct ggtgaactcc tcggccagaa tgtcgtagat cagcacatca tggtatttgc
     4261 cggccatgaa gatgaactga cggcgcaggc ccgccgcctt gaagcccact ttttcgtaac
     4321 agcggatggc gcgtttgttg aagctgtaaa cgtcgagggt gatgttgcgc aggttcagca
     4381 cgttgaagcc gtaatccagg gtgaggttcg tggcctcggt gccgatgccg cggttccagt
     4441 aggtcttgtc gccgatgaaa atgccgaact tcgcgtgcct gtgcacttcg ctgaccccca
     4501 tcaggctaca gttgccgatg gatttgttgg tgaccttttc cacaatggcg aaggaatggc
     4561 tgtcctccat gattttctgc agtaactcgc gttccttgtc caaatcgaag atggaagcgg
     4621 aaaactgcat gaagagcccc atctccatgt cgttcaccca ttcggtgtag cgctccacgt
     4681 cgtccaagga aatcggcgaa aggtagcatt tttcgccaac cagttttctg aagtatttca
     4741 ttcttatctc ccgctccccg cagcgcaggg ctttattttc atagatttaa ctgaagggaa
     4801 ataacatttg cgcgggggct atctgtcaag ctaaatccgc agtttctgct tcccgagggg
     4861 ggcggggctc aaagcagggc gcggaatccg gatattcgcg tttccctacg gactcaactg
     4921 atcaacggat tcgacggatt cttaatctgt gtaatctgtg aaatccgcgt gaacttcttc
     4981 cttgccgttc gttaacacac tctatttgct gtctgatgcg cctcccgaaa
//
LOCUS       DAOOWR010000103         5023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16837_length_5023_cov_3.836151, whole genome shotgun sequence.
ACCESSION   DAOOWR010000103 DAOOWR010000000
VERSION     DAOOWR010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16837_length_5023_cov_3.836151"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLB85_03580"
     CDS             complement(<1..1216)
                     /locus_tag="PLB85_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU72440.1"
                     /translation="MPDTKRVYLFGNGSAEGKADMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTDACNEYTQLGEARMRELLLAEVQSAVKYVEGLMHMSFGSNENPLLLSVRSGG
                     RASMPGMMDTILNLGMNDNAVNGIIKKTGNERFAWDSYRRFVQMYGDVVLGLKPASKE
                     EHDPFEVLIDEMKKEKGVKQDLELSAADLKELVARFKKAVKDNTGHDFPDDPWEQLWG
                     AIFAVFNSWNNDRAVFYRQLNGIPDDWGTAVNVQAMVFGNMGETSATGVAFTRDAATG
                     ENLFNGEYLINAQGEDVVAGIRTPQQITKIGSQRWAELAGVSEAERAAKYPSLEEAMP
                     GVYQELFATQKKLEDHTKDMQDVEFTIQEGKLWMLQTRNGKRTGFAMVKVAMDLVREG
                     KIDEKTALLRMEP"
     gene            1680..4301
                     /locus_tag="PLB85_03585"
     CDS             1680..4301
                     /locus_tag="PLB85_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU72441.1"
                     /translation="MKKLFVLLFALLCLGQIAAKVDLNTANFEELLQLPITERQARDI
                     LDYRTYISVFSDIYQLREIPSIDQPTLLRIKNLAVVSLYIEEDEVAARRDEIRDLLER
                     LDSNEGASEGMSDVWEDYLMTPQNVNKMHFDDFISLPNVSAVDAVGILTRTAKGDTIA
                     DTRDLRNSPGLSYYGYTNLRSYVYYKEPPVKNRFMWDAKLQYYTRYLEEGAYDMYHES
                     FLRSDYGNSSVTVPHRKDLSYWGYFNMEELNPDVLVKLRARYGNNYKVGWMYYSPKGE
                     TPVRDKDPAEIVADSKFYAGYENTDLPLLDNTRLKLYLGHYRATFGEGLVMENTDYYS
                     ARKTGYGFSKRILGLTPDLSRTQEYALRGAALEITNPWFGVSGWISQDNKDAVAYVDR
                     DGKVIQEGGKNKLFSYVIPTTRFGTEELLEAEAYFNNELQSGSPYATDYINLAYRKDV
                     LRETLWGTHVQVNPFLGTKLGFTTYTALYDDAHFVVPGWNDLRMLLVRDSYYYPKFKM
                     MDAEIAGLYSTLTDTYSRNYRRVLGFDAQTTISNVSVQGEYAELSVDGSDFKLGDDPK
                     AYLVSAYTQFDNLYFLSLFRHYDIGFDNPYSNSFSEHERFDDTILEKNVYTLTNPTLS
                     DIYLNASQSQPESGIYFETRYKFHRFFTIGRSYLDIWERLTDGRRSVRLQTELEFRPL
                     YQIALRARFKTQTNRYDDYAERGVSKTDEYTFSVRTFLSNRDFLEFEYRYNTVLSPPY
                     TSLTNPALPGNNTMAAAQTLMTGDYVGVNYTHNFNANLKLQGSLIYWFGHGISHWDWE
                     DMEIDFMGEKGMKAWVALHSRISRNMYLSLKFRNKTYQTQELSIRQYNVPVQGENLYQ
                     RVEHKENTIRLSLEYRY"
     gene            4314..>5023
                     /locus_tag="PLB85_03590"
     CDS             4314..>5023
                     /locus_tag="PLB85_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72442.1"
                     /translation="MIKKHIIITLAMLISLAGLSAWSISDSYFGNRFGTLDARSKAMG
                     GTGLFEDLRPAGITVNPANLTLMDKCAGITAGAFVNRNEDNRAVPLYNSFDNYIDDSV
                     YASNINFYDDYSGAAFAALKLGWARFGLGAYYKPYLSFDGNYDEQIRNNRNTDNDGYP
                     EMVAVNKLANEGRLDQASAVASMGFRLGEVFDINLGFDFGKLFGDVSNERSIRWSQWA
                     INTIVDAVGEDVLPDYTYT"
BASE COUNT         1129 a         1503 c         1327 g         1064 t
ORIGIN      
        1 tgggctccat gcgtagcagg gctgtctttt cgtcaatctt gccttcacgc acgaggtcca
       61 ttgccacttt caccatggcg aaacctgtgc gtttgccgtt gcgggtctgc agcatccaga
      121 gcttgccttc ctggatggtg aattcgacgt cctgcatatc cttggtgtgg tcctcaagct
      181 tcttctgagt ggcgaaaagc tcctgataga ccccgggcat cgcctcttcc agggagggat
      241 atttggcggc gcgttcggct tcggaaaccc cggccagttc ggcccagcgc tgcgagccta
      301 tcttcgtgat ctgctgcggg gtgcggattc ccgccaccac gtcttcgccc tgggcattga
      361 tgaggtattc gccgttgaaa aggttctcgc cggtggcggc gtcgcgggtg aaggccacgc
      421 cggtggcgct ggtttcgccc atgttgccga aaaccatggc ctgaacgttc acagcggtgc
      481 cccagtcgtc cgggatgccg ttaagctggc ggtagaagac ggcgcggtcg ttgttccagc
      541 tattgaacac ggcgaaaatc gcgccccaaa gctgttccca ggggtcatcc ggaaaatcgt
      601 ggccggtgtt gtctttcact gctttcttga agcgggccac cagttctttc agatcagccg
      661 cgctgagttc gaggtcctgt ttcacgccct tttccttttt catctcgtcg atgagcactt
      721 caaaggggtc gtgttcttct ttgctggcag gtttgaggcc cagaaccacg tcgccgtaca
      781 tctgcacgaa gcggcggtaa ctgtcccagg cgaaacgttc gttgccggtc tttttgatga
      841 tgccgttcac ggcgttgtca ttcatcccga ggttcaggat ggtgtccatc atgccgggca
      901 tggaagccct gccgccgctg cggacggaga gcagaagggg attttcgttg gagccgaaac
      961 tcatgtgcat caggccttcg acatacttca cggcgctttg cacctcggcc agaagcagtt
     1021 cccgcatcct ggcttcaccc agttgggtat attcattgca ggcgtcagtt gtgatggtga
     1081 atccgggggg cacaggcacg ccgattagat tcatctcggc gaggttggcg ccttttcctc
     1141 ccagcagatt cttcatatcc gccttgcctt cggcacttcc gttaccgaat aggtaaacac
     1201 gtttggtatc aggcatttat cctccagtga tggttagttt gcttgtttcc tgaccaattt
     1261 taaagtgggg tctttttgtc aatcattaat cctgcctcgc gagagcggag cccactgatc
     1321 ggccgttaag gccctttctt tgatttctga ctcgcctccc gaacgatgcc cgcattcgat
     1381 gcggggattg agcgaggggc gtgggagggg aaagggctcg agggctaagg gcctgaccga
     1441 tggggcatga taaggggttg gagttcatgg ttctttcaaa ttgagtggat ttcctgttgt
     1501 ttcaagcccg gagggcggca cccacctgat ttgaaagaga gccttttgaa aaacggttga
     1561 ttccagccga ggcggttctg gcagaaaaaa tagctttaca gattgtcagg catccaaaga
     1621 atgtgttttc gagaagaaat atcgagttca atgagtataa cgcatagtaa ggaaaagcga
     1681 tgaaaaagtt atttgtcttg ctctttgccc tgctttgtct ggggcagatt gccgccaaag
     1741 tggatcttaa caccgccaac ttcgaagaac ttctgcaact gccgataacg gaacggcaag
     1801 cccgggacat tctggactac cgcacctaca tctccgtttt cagcgatatt taccagctgc
     1861 gggagattcc ttccatcgac cagcccaccc tgctgcgcat caaaaacctg gcagtggttt
     1921 cgctgtacat agaggaggac gaagtggcgg ccaggcggga cgagatccgc gacctgctgg
     1981 agcgcctgga cagcaacgaa ggggcatccg aaggcatgtc cgacgtttgg gaagactatc
     2041 tgatgacgcc ccaaaacgtg aacaagatgc acttcgacga cttcatcagc ctgcccaacg
     2101 tttccgccgt cgatgcggtc ggcatcctca cccgaacagc caaaggcgac acgatcgcgg
     2161 acacacgcga cctgcgcaat tcgccggggc tcagctatta cggctacacc aacctccgca
     2221 gctacgtcta ttacaaggaa ccgccggtga aaaaccgctt catgtgggac gccaagctgc
     2281 agtattacac ccgctacctg gaggaaggcg cctacgacat gtatcacgag agcttcctgc
     2341 gcagcgatta cggcaattcc agcgtgacgg ttccccaccg caaggacctt tcctactggg
     2401 gctacttcaa catggaggaa ctcaaccccg acgtcctcgt gaagctgcgc gcccgctacg
     2461 gcaacaacta caaggtgggc tggatgtatt acagccccaa gggtgaaacc cccgtcaggg
     2521 acaaagaccc ggccgagatc gtggccgatt ccaaattcta cgcaggctac gaaaacaccg
     2581 atttgccgct gttggacaac acgcgcctga aactgtacct gggccattac cgcgccacct
     2641 tcggggaggg cctggtgatg gaaaacacgg attattacag cgcccgcaag accggctacg
     2701 gcttcagcaa acgcatcctg ggcctcactc ccgacctttc cagaacccag gaatacgccc
     2761 tgcgcggcgc cgccctcgag atcacaaacc cctggttcgg cgtttccggc tggatttcgc
     2821 aggacaacaa ggacgcggtg gcctatgtgg accgggacgg caaagtaatc caggaaggcg
     2881 gcaagaacaa gcttttcagc tatgtgatac ccaccacgcg ctttggcacg gaggaactgc
     2941 tagaagccga ggcctatttc aacaacgaac tccagagcgg ctcgccctac gccacggatt
     3001 acatcaacct cgcctaccgc aaggacgtcc tgcgcgaaac tctctgggga acgcacgtgc
     3061 aggtgaatcc cttcctgggc acaaagctgg gtttcaccac ctacacagcg ctttacgacg
     3121 acgcccactt cgtggtgccg ggatggaacg acctcaggat gctactggtg agggattctt
     3181 actactatcc caaattcaag atgatggacg ccgaaattgc cggcctctac agcaccctga
     3241 cggacaccta ctcacgcaac taccgccggg tgctgggttt cgacgcccag accaccatca
     3301 gtaacgtttc cgtgcagggt gaatacgcgg aactgagcgt ggacggctcg gatttcaagc
     3361 tgggcgacga tcccaaggcc tacctggtga gcgcctacac ccagttcgac aacctctatt
     3421 tcctcagcct cttccgccac tacgacatcg gtttcgacaa cccctacagc aattccttct
     3481 ccgagcacga acgcttcgac gacaccatcc tggaaaagaa cgtttacacg ctcactaacc
     3541 ccaccctttc ggacatctat ctgaacgcgt cccaatccca gccggaatcc ggaatatact
     3601 tcgaaacccg ctataaattc caccgcttct tcaccatcgg acgcagctac ctggacatct
     3661 gggaacgcct caccgacggc cgccgctccg tgcgcctgca aacggaactg gagttccggc
     3721 ccctttacca gatcgccctg cgcgcgcgct tcaaaaccca aaccaaccgc tatgacgact
     3781 atgccgaacg cggtgtttcc aagaccgacg aatacacctt cagcgtgcgc accttccttt
     3841 ccaaccgaga cttcctggag ttcgaatacc gctacaacac ggttctgagc cctccctaca
     3901 cctcactcac caatccggcc ctgccgggaa acaacaccat ggcggccgcc caaaccctga
     3961 tgaccggcga ttacgttggt gtaaactaca cccacaactt caacgccaac ctgaagctgc
     4021 agggctcgct catctactgg ttcgggcacg gcatttcgca ctgggattgg gaagacatgg
     4081 aaatcgactt catgggcgaa aagggcatga aagcctgggt ggccctgcat tccagaatct
     4141 cacgcaatat gtatctttct ctgaaattca gaaataaaac ctatcagacg caggaactga
     4201 gcatccgcca gtataacgtg cctgtgcaag gtgaaaacct gtatcagcgg gtggaacaca
     4261 aggaaaacac catccgcctc agccttgaat accgctacta aagtggagga ataatgataa
     4321 aaaaacatat aatcatcacg ctggcaatgc ttatttccct ggctgggctg agcgcctggt
     4381 cgatttcgga cagctatttc ggcaaccgct tcgggacgct cgacgcccgc agcaaagcca
     4441 tgggcggaac cggactgttc gaggacctgc gccccgccgg aatcacagtc aacccggcca
     4501 acctcaccct gatggacaaa tgtgcgggca tcaccgccgg agcgttcgtc aaccgcaatg
     4561 aagacaaccg cgccgtgccg ctctacaact ccttcgacaa ctacatcgac gactctgtct
     4621 atgcctccaa catcaatttc tacgacgact actccggcgc cgccttcgcc gccctcaaac
     4681 tgggctgggc aagattcggg ctgggagcct attacaagcc ctacctgagt ttcgacggta
     4741 actacgacga gcagatacgt aacaaccgca acaccgacaa cgacggctat ccggaaatgg
     4801 tggccgtaaa caagctcgcc aacgaagggc gcctggacca ggcttccgct gtggccagca
     4861 tgggcttccg gctgggcgag gtcttcgaca tcaacctggg ctttgatttc ggcaagctct
     4921 tcggcgatgt ttccaatgag aggagcatcc gctggagcca gtgggcgatc aataccatcg
     4981 tggacgcggt cggagaagac gttttgccgg attataccta cac
//
LOCUS       DAOOWR010000104         5020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_16853_length_5020_cov_3.245317, whole genome shotgun sequence.
ACCESSION   DAOOWR010000104 DAOOWR010000000
VERSION     DAOOWR010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16853_length_5020_cov_3.245317"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="PLB85_03595"
     CDS             <1..1228
                     /locus_tag="PLB85_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOU72443.1"
                     /translation="ANVIDYSALTDYRRQSILKSIAVIKLNGGLGTSMGLSAAKSLLP
                     VKGNMNFLDIITRQVLAMRSGTGYEVLLMLMNSYNTEADTLNYLEKYPDLSRQKLPIS
                     FLQNKFPRIRQDNLKPYENEDPDKMWNPPGHGDIFAALSSSGLLDKMIDAGYRYAFVS
                     NSDNLGATVDTCIPAYMEANDIHFLMEVCARTEADRKGGHLCQDKHGQLMLREVAQCP
                     PEELDEFQNVELYSYFNTNNLWIDLKALQWNLIAGEGIILLPLIVNPKTVDGTPVYQL
                     ETAMGAAISVFNGARAVLVPRSRFSPVKKNNDLLAIWSDLYELNDQYQVVMRRGVESI
                     PPIELDERYYGRIDQLLERFRDGVPSLLNCSSLKISGDISFGEDVICEGKVNLTAENP
                     VHVKSKLLSGDITFNP"
     gene            1240..2472
                     /locus_tag="PLB85_03600"
     CDS             1240..2472
                     /locus_tag="PLB85_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOU72444.1"
                     /translation="MAAKSARIRLGIFVLTGVALIGLFFALVIGKQLTLKRETYFLRF
                     TNHSVFGLQAGASVYYRGIPIGTVDKVAIDPKDVQSVILTLSIDPGTPLKEDSEAVLV
                     YVGITGTKAVEVRGGTNRAKNLKPGSFIKTGATELDEISGKAVSIVDKVDRIASNLDQ
                     LTAEENQANLSSILAETNSLIIETRTKLSSTLNSLDRIVQSAQGFTEGAGGSFDRIAD
                     NLTANLDSISTVASQNIQRIGDRSSSSLEALTDDLSKQLASISASLDKSIAEINHSTN
                     LLLNDTRLHLGKIGASSDSLILMTTRDIATVSLKINTAMDRVNQILSSAEMDALVANI
                     NTLTAKLNEANISGLAEELTVTVHKAGNLITNIDRTLIRNRVNLNETLENLREASENL
                     NDFARQISDQPALILRGN"
     gene            2485..4644
                     /locus_tag="PLB85_03605"
     CDS             2485..4644
                     /locus_tag="PLB85_03605"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOU72445.1"
                     /translation="MNKKRLWLGILAVAMVVTGCAWNPERLRPNYYVLEYREGTENPA
                     LRMSRPFPKSLEVLDAEVSRAYSKSQLVVKENFSRVRYLPDHLWASRLNDAIPDMIVQ
                     RLRAYRIFSQVDRTTGEFDPDYFLETKILNLEKIEAEDARAYLRMEFFLRDSDTQKIL
                     LSYKAERYKPLPDPSMVYLIQSYNELLMHETDVFATKCRSFLSGGQDQVTLETVETAC
                     QGLYTLEDIPPESMAGSDGELLLILGTDTDDLIRYNILGLDNDVFRNDGEFNKELTLP
                     PGRYSVTIGESQNISLEVEVLPKMRTVVSGQWAELSVKIIDGKNNRVRLSYDIWVKET
                     GKFEYYLLGPGTSIGDDDLGQPNKVWILPPGTYMVKLGGGSWTQLEDFTTIDLAKGDS
                     RVLTLVVDPSGEGNMLLGAGVLGEEPVVLDRPVVHKGAMHGNLSLAGNNEADPAHPLV
                     GITVTGQLENSVDATFPLTLYTLRSIYDLGLSSSNVSDLRISADAYSLKNVLLLTPWE
                     SSRSLRNFSFYARGDVQTHFFREYAYFSKLKDLILISAENDTVAVMTSQNRLKSKNPF
                     FPMRLKEGTGITYRLVFGPQASLSLRFGYGWQQEVNDGFYKYSESKPGQNTDYAKTYD
                     IYREQPNQTFHGLESTLVLSAVNIFNFIRVNSSLDLLFPLSLEDRSVRLENENRINFR
                     IYRNISLDLRLNIQYDRSQKDWVVYNYGSYLRLSLFY"
     gene            4648..>5020
                     /locus_tag="PLB85_03610"
     CDS             4648..>5020
                     /locus_tag="PLB85_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72446.1"
                     /translation="MAGFRFDAGTLYLEGDFTLKGLDSLRAGLAGAIKHEKVERIDLS
                     GIEQIDSAGTAAWDEILLLVSQEGEVALIPARPEVQVVINRFSNPNRIPLEKPREPSW
                     LEHIGASVQTAWQSSKQAIQLA"
BASE COUNT         1264 a         1459 c         1291 g         1006 t
ORIGIN      
        1 ggcaaatgtg attgattatt ccgccttgac agactacagg cgtcaatcca ttttgaaaag
       61 catcgccgtg atcaagctga acggcggcct gggcaccagc atgggcctca gcgcggcgaa
      121 aagccttctg ccagtgaagg ggaacatgaa ctttctggac ataatcaccc gtcaggtgct
      181 ggccatgcgt tccgggaccg gctatgaagt gctgctgatg ttgatgaaca gctataacac
      241 cgaggccgac acgctgaatt atctggaaaa ataccccgac ctcagtcggc agaagctgcc
      301 gatatccttt ctgcaaaaca aattccccag aattcgtcag gacaacctga aaccctatga
      361 gaacgaagac ccagacaaga tgtggaaccc gcccgggcat ggagacatct ttgccgcatt
      421 gagttcctcg ggattgctgg acaaaatgat cgacgccggc taccgctatg cctttgtgtc
      481 aaattctgac aaccttggcg caaccgtgga cacctgcatc cctgcctaca tggaggccaa
      541 cgacatccat ttcctgatgg aggtttgcgc ccgcacggaa gcggacagga aaggcggtca
      601 cctctgccag gacaaacacg gccaactgat gctgcgggaa gtggcgcaat gccctccgga
      661 agagctggac gaattccaga acgtggagct ttactcctat ttcaacacca acaacctctg
      721 gatcgacctc aaagcgctgc agtggaacct gatcgccggc gaggggatca tcttactgcc
      781 gctgatagtg aatcccaaaa ccgttgacgg cacccctgtc tatcagctgg aaaccgccat
      841 gggggccgcg atcagcgtgt tcaacggcgc ccgggccgtt ttagtgccga ggtccagatt
      901 ctccccggtg aaaaaaaaca acgacctgct ggctatctgg agcgaccttt acgaacttaa
      961 cgaccagtat caggtggtga tgaggcgtgg ggtggaatcc attccgccga tcgaactgga
     1021 tgaacgctac tacggcagaa tagaccaatt gctggaacgc tttcgcgatg gcgtcccctc
     1081 gcttctgaac tgctccagcc tgaagatcag cggcgacatc agtttcggcg aggatgttat
     1141 ctgcgaaggc aaggtgaatc tgaccgcgga aaaccctgtg cacgttaaaa gcaaactgct
     1201 gtctggagac atcaccttca atccctgacg ggaggtatca tggctgccaa atcagcccgg
     1261 atcagactgg gcatctttgt gctgaccggc gtcgccctga tcggcctgtt ttttgcgctg
     1321 gtgatcggca aacaactcac cctaaagcgt gaaacctact ttctgcgatt caccaaccat
     1381 tcggtattcg gcctccaggc cggtgcctca gtctattaca gaggcatacc catcggcacc
     1441 gtggacaagg tggcgatcga tcccaaggat gttcagagcg tgatcctaac actgagcatc
     1501 gaccccggaa ccccactgaa agaggatagc gaagctgttc tggtgtatgt gggcatcacc
     1561 ggcaccaaag cggtggaggt tcgcggtgga accaatcggg cgaaaaacct gaaacccggc
     1621 agcttcatca aaaccggcgc tactgagctt gacgagattt ccggcaaggc tgtttccatc
     1681 gtggacaaag tggaccgcat cgcctctaac ctggaccagc tgacagcgga ggaaaaccag
     1741 gccaacctct cttccatcct tgctgaaacc aacagcctga tcatcgaaac ccgcaccaaa
     1801 ctttcctcaa ccctgaacag cctggaccgg atcgtccaat ccgcccaggg cttcacagag
     1861 ggcgcgggag gaagttttga ccgcatagca gacaacctga ccgcgaacct ggacagcata
     1921 tctaccgtgg cttcccaaaa tatccaaagg atcggcgacc gcagctcctc cagcctggaa
     1981 gcccttaccg acgatctgag caaacaactg gcttccatca gcgccagcct cgataaaagc
     2041 atcgctgaga tcaatcactc aaccaatctg ctactcaacg ataccaggct gcacctggga
     2101 aagatcggcg ccagttccga ttccctcatc ctgatgacca ccagggacat cgccacggtt
     2161 agcctgaaga tcaacacagc catggaccgc gtgaaccaaa tcctctcctc agctgaaatg
     2221 gacgctctgg tagccaacat aaacactctc acggccaaac tcaatgaggc caatatctcc
     2281 ggtctagctg aggaactgac agtaacagta cacaaagccg gaaacctgat aaccaatatc
     2341 gaccggaccc tcatccgcaa ccgggtaaat ctgaatgaaa ccctggaaaa cctgcgcgaa
     2401 gccagcgaaa acctcaatga tttcgcccgg caaatatctg accaacccgc cctgatcctg
     2461 cggggaaatt aaagggagac gcttgtgaac aagaaacgtt tgtggctggg catccttgcc
     2521 gtcgcgatgg tcgtgactgg ctgcgcctgg aatcctgagc ggctgcgtcc aaattactat
     2581 gtgctggaat acagggaggg aaccgaaaac cccgctctac gcatgtcccg gcccttcccc
     2641 aaatccctgg aggtgctgga tgcggaagtg agccgggctt acagtaaaag ccagttggtg
     2701 gttaaagaaa acttctcccg ggtgcgctat ctgcccgatc atctttgggc cagccggctc
     2761 aacgacgcca ttccagacat gatcgtgcaa aggcttcgcg cctaccgcat cttcagccag
     2821 gttgaccgca ccacaggaga attcgatccc gactactttt tagagaccaa gattctgaac
     2881 ctggaaaaga tcgaggcaga ggatgcccgc gcctatttac ggatggagtt cttcctacgg
     2941 gattccgaca ctcagaagat cctgctcagc tataaggcgg agcgctacaa acccctgccc
     3001 gaccccagca tggtctatct gatccagagc tacaacgagc tgctgatgca tgaaactgac
     3061 gtattcgcga ccaaatgcag gtctttcctg agcggtggac aggaccaggt gaccctggaa
     3121 accgtcgaaa cagcatgcca aggcctctac acgctggaag atattccccc ggaaagtatg
     3181 gccggttctg acggtgaact gctgctcatt ctcggcacgg acaccgacga ccttatccgc
     3241 tacaacatcc ttggcctgga caacgatgtt ttccgcaatg acggagaatt caacaaggag
     3301 cttaccctcc ccccgggccg atacagcgtc accatcggcg aaagccagaa catatcgctg
     3361 gaagtggagg ttctacccaa gatgcgcacc gtggtgagcg ggcaatgggc agaactgagc
     3421 gtcaaaatca tcgacggaaa aaacaaccgg gtgcgccttt cctatgacat ctgggtaaag
     3481 gaaaccggca aattcgaata ctatctcctt ggccccggaa ccagtatcgg cgatgacgac
     3541 ctgggccagc caaacaaggt ttggatcctt ccccccggca cctacatggt caaactgggc
     3601 ggcggctcct ggacccaact ggaggacttc accactatcg atctggccaa aggcgacagc
     3661 cgggtcctga ccctggtggt tgacccttcc ggagaaggta acatgctgtt gggggcagga
     3721 gttttgggtg aggaacctgt tgtcctggac cggcctgtgg ttcataaagg cgcaatgcac
     3781 ggaaatctga gcctggccgg gaacaacgaa gcggaccccg cccatcccct ggtaggcatc
     3841 accgtaaccg ggcaactgga aaactcggtg gacgcaacct tccctttaac cctctacacc
     3901 ctgcgcagca tctatgattt gggccttagc agttccaatg tttccgatct gcgcatcagt
     3961 gccgacgcct attccctgaa aaacgtcctg ctgctgacgc cttgggaaag cagccgctcc
     4021 ctgcgcaatt tttccttcta cgcccgtggc gacgtgcaga cccatttctt tagggaatac
     4081 gcttatttct ccaagttgaa ggaccttatc ctcatctccg cggagaacga caccgtggcg
     4141 gtgatgacca gccagaaccg gctgaagtcc aaaaacccct tcttccccat gcgcctgaag
     4201 gagggaacgg gaatcacata ccgcctcgtt tttggccctc aagcttctct cagcctgcgc
     4261 tttggttatg gctggcagca ggaggtaaac gatggttttt acaaatacag tgaaagcaaa
     4321 cctggccaga acaccgatta cgccaagact tatgacatct acagggaaca accgaaccag
     4381 acctttcacg gtctcgaaag cactctggtg ctttcagcgg tcaacatctt taacttcatc
     4441 agggtcaatt cctccctcga cctgctcttt cccctcagct tggaagaccg cagcgtccgc
     4501 ctggagaacg aaaaccgcat caatttccgg atttaccgca acatttcgct ggacctgagg
     4561 ctgaacatac agtacgatag gtctcaaaaa gattgggtgg tttacaatta tggcagctac
     4621 ctgaggctat cgctgttcta ctgacccatg gccggatttc gttttgacgc tggcaccctt
     4681 tatctagagg gagatttcac cctgaagggg ctggacagcc tccgtgccgg gcttgctgga
     4741 gctatcaagc atgaaaaagt ggaaaggatt gacctttccg gcatcgaaca aatcgacagt
     4801 gccggaacag ccgcgtggga tgaaatcctg ttgctggttt cacaggaggg cgaggtcgca
     4861 ttgataccgg ctcgtccgga agtgcaggtt gtaatcaaca gattcagcaa cccgaacaga
     4921 attccgctgg aaaaaccccg cgaaccaagc tggctggagc atatcggagc ctcggtgcaa
     4981 acagcctggc aaagctcgaa acaagccatc caactggcgg
//
LOCUS       DAOOWR010000105         4972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17046_length_4972_cov_3.900346, whole genome shotgun sequence.
ACCESSION   DAOOWR010000105 DAOOWR010000000
VERSION     DAOOWR010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17046_length_4972_cov_3.900346"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="add"
                     /locus_tag="PLB85_03615"
     CDS             <1..1063
                     /gene="add"
                     /locus_tag="PLB85_03615"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOU72447.1"
                     /translation="REFIQKLPKTDLHVHLDGSVRIDTIIDLAKQFKINLPTLDPDEL
                     RDLIVCGDHTESLEDYLSGFGIVNLVLQNKEGLRRAAYELAEDAAKENVRYMEVRYSP
                     ILHTNKGLKLTEISQAVIDGLKQGERDFGVKTAVIICGLRNMDPTTSVKLAELAIAFK
                     NKGVIGFDLAGGEYNNPAKDHKEAFDLSLKNNLNITIHAGEAYGPDSIHQALHYCGAH
                     RIGHGTRLVEDGDLLNYVNDHRIPLEICLKSNFHTKAVPDIRSHPIDFYIDFGLRVTI
                     NTDNRTISNTTLTDEYLLAINELKLDYPTIKNVLLNGFKSAFIPYKERVRLINSILKE
                     LDEIEEDELKTKIKIRENL"
     gene            1066..1662
                     /gene="gmhA"
                     /locus_tag="PLB85_03620"
     CDS             1066..1662
                     /gene="gmhA"
                     /locus_tag="PLB85_03620"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOU72448.1"
                     /translation="MRELIQASCTATSHALETFLADDDNFVAIENIARLIARRFDCGG
                     KVIVFGNGGSMCDAMHFAEELTGRFRRDRKALPAIALSDPSHITCVGNDYGFEQVFAR
                     GVEAYAVAEDVVLGISTSGNSPNVIRALETARELGCHTIALLGRDGGRLAGSCEIQII
                     VPGEHSDRIQELHTLILHIIIECVERLLFADASGDIRP"
     gene            1659..2696
                     /locus_tag="PLB85_03625"
     CDS             1659..2696
                     /locus_tag="PLB85_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU72449.1"
                     /translation="MKNMILTLLLLLAAAFACAQGNYYDNVINLSGQQLYSALRTLIS
                     TNTNSDYGAAKEFLFQQLDNHNGYVTCIYTGEEYYVGYNYTGSNNPNTEHTYAQSWFS
                     SNESSRKKADLHHLFPSNSVVNSSRGNYPLFTVANHASANVYYNDTPWQSYRGQSSGG
                     YTVFEPADESKGNIARALLYFNTRYNDSLTQQNVYMTPVLVQWHFADPPDAAEIARNN
                     GIYGFQTNRNPYVDHPEFVGRIWGGVAVDEETASPAAELSLDRVWPNPFSDSTTLSVQ
                     SKEARQASVSVHDVKGRIVSSWSQVLTAGNSEISWNGRDKEGQKAAPGVYLIRLHSDG
                     LNASAKVLMSQ"
     gene            3262..>4972
                     /locus_tag="PLB85_03630"
     CDS             3262..>4972
                     /locus_tag="PLB85_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOU72450.1"
                     /translation="MNAQKFTLKSQEALQAMQALAEEYGHQEIRDLHLLAALLRQSDS
                     LVLPALQKLEVNLSKLTGDLENALAKLPRVSGSQVYLSQEVLDVMHQAEKDADKLQDD
                     YISLEHLLLALLEKGKEAGKLLKSMGASPDKLLLALKELRGNQRVTDQNPEAKYQALE
                     KYARNLTRLARQEKLDPVIGRDEEIRRSVQILSRRRKNNPILIGEPGVGKTAIVEGLA
                     RRIVARDVPENLKNKELLELDMAALLAGAKFRGEFEERFKAVLSEVEKSDGQIILFID
                     EIHTVVGAGAAEGAVDASNMLKPALARGSLHCIGATTIDEYRKYIEKDPALERRFQPV
                     LVQEPSVEDTISILRGIREKYEVHHGVQITDTALVSAAVMSDRYISDRFLPDKAIDLI
                     DEACAKLRMEIDSLPSELDEIERKLRQLEIERLSLAKEEDSLSKQRLEKVVAETAQLS
                     EERNALRLRWENEKKGFERTRELRAEIDSLKGQAEKAEREGDLETVSKLRYGSIKEKE
                     KELAELIQAQDHAGDTEPLIKERVDEELIAEVVSKWTNIPVSKLVQSEMSKLLGLEEI
                     LARR"
BASE COUNT         1261 a         1392 c         1366 g          953 t
ORIGIN      
        1 ccgggagttc atccaaaaac tccccaagac agacctgcac gttcacctgg atggaagcgt
       61 gcgcatcgac acgatcatag atcttgccaa gcaattcaag atcaatctgc ccactctgga
      121 cccggacgag ttgcgcgacc tgatcgtttg cggcgaccac accgaaagcc tggaagacta
      181 tttgagtggc ttcgggatcg tgaacctggt gcttcagaac aaggaaggcc tgcgccgcgc
      241 ggcctatgaa ctggccgagg acgccgccaa agagaacgtg cgctatatgg aagtgcgcta
      301 ttccccaata ctgcatacaa ataagggcct caaactcacc gagatatcgc aggctgtaat
      361 cgacgggctc aaacaggggg aacgagattt tggagtcaag accgctgtta tcatttgcgg
      421 tttgcgcaac atggacccca ccacctcggt gaagctggca gaattggcta tcgccttcaa
      481 aaacaagggc gtgatcggct ttgacctggc cggaggcgag tacaacaacc ccgccaagga
      541 ccacaaggaa gccttcgacc tttcgctgaa aaacaatctc aacatcacca tccacgccgg
      601 cgaggcctat ggtcctgaca gcatccacca ggcgcttcat tattgcggcg cgcaccgcat
      661 cgggcacggc acccggctgg tggaggacgg cgatttgctg aactatgtga acgaccaccg
      721 catccctctg gagatctgcc tcaagagcaa tttccacacc aaggcggtgc cggatatccg
      781 cagccacccc atcgactttt acatcgactt cggcctccga gtgaccatca acacagacaa
      841 ccgaaccatt tcaaacacca ccctcaccga cgaatatctg ctggccatta acgagctcaa
      901 actggattac cccaccatta aaaacgtgct cctgaacggt ttcaagagcg ccttcatacc
      961 atataaagag cgcgtgcgcc tgatcaattc catcctgaag gagctggacg agatcgagga
     1021 agacgagctg aaaaccaaaa tcaagattcg ggaaaacctc tgaacatgcg tgaactcatc
     1081 caagcttcct gcaccgctac cagccacgcc ctggagacat ttctggctga tgatgacaat
     1141 ttcgtcgcca tcgaaaacat agccaggctg atcgcccgga gatttgactg cggcggcaag
     1201 gtgatagttt tcggaaacgg cggctcgatg tgcgacgcga tgcacttcgc ggaagagctt
     1261 acaggccgct ttcgccggga ccgcaaggct ctgcccgcca tagcgttgtc tgaccccagc
     1321 cacatcacct gtgtgggcaa tgactacggt ttcgaacagg tcttcgcccg gggcgtggaa
     1381 gcttacgccg tggcggagga cgtggtgctg ggaatttcca caagtggcaa ttcacccaac
     1441 gtgatccgcg ccctggaaac cgcgcgggaa ctgggctgcc acaccatcgc cctgctaggg
     1501 agggatggag gcaggctcgc cggaagctgc gaaatccaga taatcgtgcc cggcgaacac
     1561 agcgacagga tccaggaact gcatacccta atcctgcaca tcatcattga atgcgtggag
     1621 cggctgctct ttgcggacgc ttccggagac ataagaccat gaagaatatg atccttaccc
     1681 tgctcctgct gctggctgcg gctttcgcct gcgcccaggg caattactac gataacgtga
     1741 taaacctcag cggacagcaa ctctattccg cgctgcgcac cctcatctcc acgaacacca
     1801 acagcgatta cggagcagcc aaagagtttc tatttcagca acttgacaac cacaacggct
     1861 atgtgacctg catctatacc ggcgaagaat actacgttgg ctacaactac acaggctcca
     1921 acaacccaaa taccgagcac acctacgccc aaagctggtt cagttcgaat gaatccagcc
     1981 ggaaaaaggc cgatttgcac cacctctttc cctccaattc ggtggtgaac agctcgcggg
     2041 gcaattatcc cctgttcacg gtggccaatc acgcctccgc gaacgtttat tacaacgaca
     2101 ccccctggca gagctaccgt ggccaaagct caggcggcta cacggttttc gaaccggccg
     2161 acgagagcaa gggcaacatc gcccgtgccc tgctttattt caatacccgc tataacgaca
     2221 gcctcaccca gcaaaacgtg tacatgacac cggtgctggt gcagtggcat ttcgccgatc
     2281 cgcccgacgc cgccgaaatc gcccgcaaca acggcattta cggctttcag accaaccgca
     2341 acccctatgt ggaccatccg gaattcgtgg gccgcatctg gggcggggtg gcagtagatg
     2401 aagaaaccgc ttctccagcg gcggaactga gtttggaccg ggtttggcca aatccgtttt
     2461 cggattccac caccctgtcc gtgcaaagca aggaagcccg ccaggccagc gtttccgtgc
     2521 atgatgtgaa aggccggatt gtgagctcct ggagccaggt gcttactgcc ggaaattctg
     2581 aaatatcttg gaacggcagg gataaggagg ggcaaaaagc cgctcccgga gtatatttga
     2641 tccgcttgca cagcgatggg cttaatgcct cggccaaagt gctgatgagc cagtgaacgg
     2701 aaaatggatt aggtaaacgg ataagaattt caaaaacgaa gccgggacga tggtcccagt
     2761 ttttgtttgt gccggcatta tgatttggga atctctttca aatcaaggag gttagccaag
     2821 ttgcggcaaa cgaaagtggg gatgtgaacg gtcgggaaac cccgtaaggg aatgatgcta
     2881 tgcagcttgg ccgttaaggc cttgtcccaa acctcaaata cgcctcacgc ataatgcccg
     2941 catttgatgc gaggatattg cgaggggcgt gggaggggga tggtctcggg ggttaagggt
     3001 gtagctggct ggaagcttcg gtgggtaagc atttctgtaa tccttcgggc attacccatc
     3061 cgtttggaag agaactggat ttagcatgac gaaaaaagac aataatctgc cgctctgcgg
     3121 ccttcctgat aggtgtaaac actgctttac cgcgttcttc tccaattagt tccataaatt
     3181 tagcaatcta ctactgagac tgctaaaata tatcttgaca tcgcctcgga cccggctatt
     3241 atgtccttaa aaggaggtgc catgaacgca cagaaattta ctctcaagag ccaggaagcg
     3301 ctgcaggcga tgcaggctct ggctgaagaa tacggacatc aggagatacg ggacctgcat
     3361 ctgctggctg cgctgctgcg gcagagcgat tcgctggttc tgcctgccct gcaaaagctg
     3421 gaggtgaacc tctccaagct cacgggcgat ttggagaacg ccctggccaa actaccccgg
     3481 gtgagcggtt cccaagtata tctctcccag gaagttctgg atgtgatgca tcaggcggag
     3541 aaagacgcgg acaagctgca ggacgattac atcagcctgg agcatctgct gctggctctg
     3601 ctggaaaagg gcaaagaggc gggcaagctg ctcaagtcca tgggggcgag cccggacaag
     3661 ttattgcttg ccttgaaaga acttagaggt aaccagcggg ttacggacca gaatcccgag
     3721 gccaaatacc aggccttgga aaagtacgct cgcaacctta cccgcctggc gaggcaggag
     3781 aagctggacc ccgtgatcgg gcgcgatgaa gagattcgcc gcagcgtgca aatcctttcc
     3841 cgccgccgca aaaacaaccc catcctgatc ggcgagccgg gcgtgggcaa aaccgccatt
     3901 gtggaaggcc ttgcccgccg cattgtggcg cgcgacgttc cagaaaacct gaaaaacaag
     3961 gagcttcttg agctggacat ggccgcgtta ctggccgggg ctaagttccg cggcgaattt
     4021 gaagaacgct tcaaagcagt cctgtccgaa gtggagaaat ccgatggcca gataatcctc
     4081 ttcatcgacg aaatccacac cgtggtgggg gccggagccg ccgagggcgc ggtggatgct
     4141 tcgaacatgt taaaacccgc cctggcaagg ggttcgttgc actgtatcgg cgccaccacc
     4201 attgacgaat accgcaaata cattgaaaag gaccccgccc tcgaacgccg cttccaaccg
     4261 gtgctggtac aggaacccag cgtggaggac accatctcca tcctgcgcgg catccgcgaa
     4321 aaatatgagg tgcaccacgg tgtccagatc accgacacag cgttggtttc cgccgcagtg
     4381 atgagcgatc gctacatttc cgaccgcttt ttgccagaca aggccatcga cctcatcgac
     4441 gaggcctgtg ccaagctgcg catggagata gacagcctgc ccagcgaact tgacgaaatc
     4501 gagcgcaagc tgcgccagtt ggaaatcgag aggttgtccc tggccaagga ggaggactcg
     4561 ctttccaaac agcggctgga aaaagttgtg gctgagacag ctcaactgag cgaggaacgg
     4621 aacgccctga gattgcgctg ggaaaacgaa aagaagggct ttgaacggac ccgcgagctg
     4681 cgcgctgaaa ttgactccct caaaggacag gccgaaaagg ctgaacgcga gggcgacctg
     4741 gaaaccgttt ccaaactccg ttatggctct atcaaggaaa aggagaaaga gcttgcggaa
     4801 cttattcagg cacaggatca tgctggtgac acagagccgc tcatcaaaga aagggtggac
     4861 gaggaactga tcgcggaagt ggtttccaaa tggaccaaca tcccggtctc aaaactggtg
     4921 caaagcgaga tgagcaaatt gctgggactg gaggagatac tggccaggcg cg
//
LOCUS       DAOOWR010000106         4962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17086_length_4962_cov_3.915835, whole genome shotgun sequence.
ACCESSION   DAOOWR010000106 DAOOWR010000000
VERSION     DAOOWR010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17086_length_4962_cov_3.915835"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLB85_03635"
     CDS             complement(<1..253)
                     /locus_tag="PLB85_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOU72451.1"
                     /translation="MKFYRSHILVGINETSLLAGVENFIVALRAELAKYNLQEEINIL
                     ETGPLGFFGRGICLSVYPENVSYQDVKAEDIPELVEEHFL"
     gene            complement(250..708)
                     /gene="nuoE"
                     /locus_tag="PLB85_03640"
     CDS             complement(250..708)
                     /gene="nuoE"
                     /locus_tag="PLB85_03640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU72452.1"
                     /translation="MYKEICTKYTPDKDNLICILHEIQDTHPQHYISEEAVQTVAEYL
                     NLPPNHIYGVLTFYTMYSTKPRGKNIIRLCESPPCYIKGSENILRKLRELLGVKTGET
                     TKDGVFTLELCACLGVCGNAPVMMVNNDVYGDLTEERVEEIIARIRRGNQ"
     gene            complement(721..2568)
                     /locus_tag="PLB85_03645"
     CDS             complement(721..2568)
                     /locus_tag="PLB85_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU72453.1"
                     /translation="MSLKRIDLLICCGSGCVSAGALKIKERFHEVLADKGIANEVNII
                     ETGCMGPCDYGPVMVVYPEGTFYKHVKVEDVEEIVSEHFVKGRPVQRLMLQEEERLIA
                     AQKEVPFYQKQVKVALANCGYIDPESLEEYIATGGYEALGTVLTQMKPEEAIEVVKKS
                     GLRGRGGGGFPTHIKWRLVHDIKADQKYIICNGDEGDPGAFMDRSLLEGDPHRILEGM
                     MLGAYSIGASKGFFYIRAEYPLAIKRIKQAIAQAKEVGLMGENIFGSDFSFDAEVRTG
                     AGAFVCGEEMALIHSLEGERGNPTPKPPYPAVKGLWEKPTVVNNVETMANLPTIFLKG
                     AEWFSSMGTETSKGTKVFALTGDIQNTGLVEVPMGITLREMIFDVGGGMTDGHKFKAV
                     QLGGPSGGCLTAEHLDVPVDYESLKERGAMMGSGGVIVMNDQKCMVNVAKFFVDFCVD
                     ESCGKCPPCRLGLKQMYNILDRITKGQGKESDLDELLRLGETISKLSLCGLGQTAPNP
                     VLSTLRYFREEYEAHIRDKSCPNKVCTDLFHFSIDKDRCIGCSLCSRKCPATCISGSR
                     EEKFTINQVDCVKCGNCQDVCPVKAIDRIPGMHPEVVRHAEDLAKSEEE"
     gene            complement(2584..2949)
                     /locus_tag="PLB85_03650"
     CDS             complement(2584..2949)
                     /locus_tag="PLB85_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOU72454.1"
                     /translation="MKTLADLKKIREKAQEEMKLRGGNVRVKIVVGMGTSGIAMGARE
                     VMKTFLEEISNRNLTDVLVTQTGEKGLSSMEPVVDLIEEGKAKVTYGNMNPDKVRKVV
                     VEHIVNGRVVSDYVVATGD"
     gene            complement(2949..3518)
                     /locus_tag="PLB85_03655"
     CDS             complement(2949..3518)
                     /locus_tag="PLB85_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72455.1"
                     /translation="MQDISLHLLDIIENSVRAKARNVKIRVIKDVGENLLRLIVEDDG
                     SGMDSETLAMAQNPFYTSKAQREKKVGLGIPLFKQNAELSNGSFNMSSQPGFGTVLTV
                     DFGLNHIDRMPLGNLKATLLGAIVGHPEVDFQIILINHDGGKENSFHFETAAIKEELG
                     DIPLTYPDVIEYIDQSLNEGIQNTNMENV"
     gene            complement(3528..4010)
                     /locus_tag="PLB85_03660"
     CDS             complement(3528..4010)
                     /locus_tag="PLB85_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU72456.1"
                     /translation="MTPATQQDNPLYDRLRAVIAASRGLRNPLIEVLRQAQEIFGYLP
                     VEVQEFISREMDIPANKIYGVVTFYNFFTMIPRGKYTLNLCMGTACFVKGAPRLARMI
                     EEELGVKMGETTADLRFTVSAVRCVGACSLAPVFVIGEETYGRVDTREKVSEILKKYQ
                     "
     gene            complement(4032..4769)
                     /locus_tag="PLB85_03665"
     CDS             complement(4032..4769)
                     /locus_tag="PLB85_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU72457.1"
                     /translation="MRWFKADLHIHSVLSPCGGLEMSPREVVARAKSCGIDWMAITDH
                     NSLANCPAYSAVAEREGLAFTWGVEMQTAEEIHLLVYFDDPAAAKSFGGELYASLLPL
                     DNDPEFFGDQVIIDENENILGMELRALMNSSIWDLSTAVEVARNHGGYCVPAHVDATV
                     NSLIGQLGFLPAEPYFELLGLSAKADLARLLSSHPELAGKSWLRSSDAHYLADMGSGF
                     SRVLAGAPKVEELAKAALGLDDRMILV"
     gene            complement(4773..>4962)
                     /locus_tag="PLB85_03670"
     CDS             complement(4773..>4962)
                     /locus_tag="PLB85_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72458.1"
                     /translation="KHLNTVAVASLTGIPAIVFAKGNTPDEMVVEKAASEGICLVSSP
                     LDTFSLAGILYGMLHQLI"
BASE COUNT          940 a         1359 c         1421 g         1242 t
ORIGIN      
        1 tgaggaaatg ctcttccacg agttcgggga tgtcctctgc ctttacgtct tggtaactta
       61 cgttttccgg atagacggag aggcaaatcc cgcggccgaa aaagcccagg gggccggtct
      121 cgaggatgtt gatctcttcc tggaggttgt attttgccag ttcggcgcgg agggcgacga
      181 tgaagttttc cactccggcc agcagcgagg tttcatttat gcccacaagg atgtggctgc
      241 gatagaattt cattggttcc cccttctgat cctggcgatg atttcctcca cccgttcttc
      301 ggtaaggtcg ccgtaaacat cgttgttcac catcattacg ggggcgtttc cgcaaacgcc
      361 gaggcaggcg cagagttcca gggtgaaaac cccgtctttg gtggtttcgc ctgttttgac
      421 gccgaggagt tccctgagct tgcggaggat gttttcgctg cctttgatat agcagggcgg
      481 cgattcgcag aggcggatga tgttctttcc ccggggtttg gtgctgtaca tggtgtagaa
      541 ggtcagcacg ccgtagatgt ggttgggcgg cagattcaga tattccgcca ccgtctgcac
      601 cgcctcttcg gagatgtaat gctgtgggtg ggtgtcctgt atctcgtgca ggatgcagat
      661 caggttgtct ttgtcaggag tgtatttggt gcaaatctct ttgtacatgg gtactccttg
      721 tcattcttct tctgatttgg cgaggtcttc cgcgtgcctg accacttcgg ggtgcattcc
      781 gggaatcctg tcgatggcct ttacaggaca gacatcctga cagttcccgc atttaacgca
      841 atccacttgg ttgatggtga atttttcctc gcgtgaaccg ctgatgcagg tcgccgggca
      901 tttgcgtgag cagagcgagc agccgatgca gcggtctttg tcgatgctga aatggaagag
      961 gtctgtgcag accttgttgg ggcaggattt gtcgcggata tgggcttcat attcttcgcg
     1021 gaaatagcga agggtggaaa ggacggggtt cggcgctgtc tggccaagcc cgcagagcga
     1081 gagcttgctg atggtttcgc caaggcgcag cagttcgtcg aggtcgcttt ccttcccctg
     1141 gcctttggtg atgcggtcca ggatgttgta catctgcttg aggccgagtc ggcagggtgg
     1201 acattttccg caggattcgt ccacgcagaa atccacgaag aacttggcca cgttgaccat
     1261 gcacttctgg tcgttcatca cgatgactcc gccggaaccc atcatggcgc cgcgttcctt
     1321 gaggctttca tagtccaccg gcacgtcgag gtgttcggcg gtgaggcagc ctccggaagg
     1381 gcctccgagc tggacggctt tgaacttgtg tccgtcggtc attccgccgc ctacgtcaaa
     1441 gatcatctca cgcagggtga ttcccatcgg aacttccacc agaccggtgt tttggatgtc
     1501 gccggtgagg gcgaagacct tggtgccctt gctggtctcg gttcccatgg aggaaaacca
     1561 ttccgcgcct ttgaggaaga tggtgggaag gttggccatg gtttccacgt tgttcaccac
     1621 ggtgggcttt tcccaaagtc ccttcactgc cgggtagggc ggtttggggg tggggtttcc
     1681 gcgctcgcct tccaatgaat ggattagggc catttcctct ccgcagacaa aggctccggc
     1741 gcctgtgcgg acttcggcgt cgaaagagaa atcggagccg aagatgtttt cgcccatcag
     1801 gccaacttcc ttggcctggg cgatcgcctg tttgatgcgt ttgatggcca gcggatattc
     1861 cgcgcggatg tagaaaaagc ctttggaggc tccgatggaa taggcgccca gcatcatccc
     1921 ttccaggatg cggtgagggt cgccttccag tagggagcgg tccatgaagg cgccggggtc
     1981 tccctcgtca ccgttgcaga tgatgtactt ctggtcggct ttgatgtcgt gcaccagtct
     2041 ccacttgatg tgggtgggga agccgccgcc gccgcgtccg cggaggccgg attttttcac
     2101 cacctcgatg gcttcttcag gcttcatctg ggtgagcacg gttcccaaag cctcgtatcc
     2161 gccggtggcg atgtattctt ccaggctttc ggggtcgatg tagccgcagt tggcaagggc
     2221 gaccttgact tgcttctggt aaaagggcac ttccttttgg gcggcgatca gcctttcctc
     2281 ttcctgaagc atcaggcgct ggactggacg gcctttcaca aaatgttcgg atacgatctc
     2341 ctccacatcc tccaccttca cgtgtttgta gaaggtgcct tcgggataga ccaccatgac
     2401 agggccgtag tcgcaggggc ccatgcagcc ggtttcgatg atgttcactt cgttggcgat
     2461 gcctttatcc gccagaactt cgtgaaaccg ttccttgatt ttcaaagcgc ctgcagacac
     2521 acagccggag ccgcaacaga tcagcaggtc aattcttttc agagacattg atcaacctcc
     2581 agctcagtcg ccagtcgcaa cgacgtaatc ggagacaacc ctgccattga cgatgtgctc
     2641 gacgaccacc tttctcacct tgtccggatt catgtttcca taggtgactt tggctttgcc
     2701 ctcttcgatg aggtcaacga ccggctccat cgaggaaagg cccttttcac cggtctgggt
     2761 taccaaaacg tcggtgaggt tgcggttgga aatctcttcc agaaaggtct tcatgacttc
     2821 acgggcgccc atcgcgatgc cggatgttcc catccccacc acgattttga cgcgcacgtt
     2881 gccgccacga agtttcatct cttcctgggc tttttcacgg atttttttca ggtctgcaag
     2941 tgttttcatc agacattctc catgtttgta ttttgtattc cttcatttaa agattggtcg
     3001 atgtactcaa tcacgtccgg ataggtgagc ggaatatcgc ccaattcctc tttgatggcg
     3061 gcagtttcaa agtggaagga gttttccttg ccgccgtcgt ggttgatgag gatgatttgg
     3121 aaatccactt ccggatggcc gactatcgct cccagcaaag tggctttgag attgcccagg
     3181 ggcatgcggt cgatgtggtt gagcccgaaa tccaccgtta gcacggttcc gaagccgggc
     3241 tggctgctca tgttgaaaga gccgttgctg agttccgcgt tctgcttgaa caatgggata
     3301 cccagcccca ctttcttttc ccgctgcgcc ttgctggtgt aaaaagggtt ttgggccatg
     3361 gccagggtct cgctgtccat ccccgagccg tcgtcctcaa cgataagccg gagaaggttt
     3421 tcgcccacat ctttgatgac gcggatcttc acatttctgg ccttggcccg gacgctgttt
     3481 tcgatgatgt ccagcagatg cagggaaatg tcttgcatca cgccggccta ttgatatttt
     3541 ttgagtattt cggacacctt ttcccgagtg tccacccggc cgtaggtttc ttcaccgatc
     3601 acgaagaccg gagccagcga gcaggctcca acgcagcgaa ccgcgctcac tgtgaagcgc
     3661 aggtcggctg tcgtctcgcc cattttaacg cccagttcct cttcgatcat ccgggccagg
     3721 cggggcgcgc ctttcacaaa gcaggccgtg cccatgcaca ggttcagggt gtatttgccc
     3781 cgggggatca tggtgaagaa gttgtagaat gtcaccactc cgtagatctt gttggccggg
     3841 atgtccattt cccgggagat gaattcctgc acttcgacgg gcaggtagcc aaaaatctcc
     3901 tgagcctggc gcagaacctc gatgagggga ttgcgcaggc cgcggctggc tgcgatgacg
     3961 gctctcagcc ggtcatacag cggattgtct tgctgtgtag cgggagtcat taactcctcc
     4021 tgttttattt tttatactaa aatcatcctg tcatcaaggc ccaacgccgc cttggcaagc
     4081 tcttccacct tgggagcccc tgccagaacc cggctgaagc cggagcccat atcggccagg
     4141 taatgcgcgt ctgaactgcg cagccagctt ttcccggcaa gttccggatg gctgctgagc
     4201 aaacgcgcca ggtccgcttt ggctgagagg ccgaggagct cgaaataagg ctccgcgggc
     4261 aaaaaaccca gttgtccgat aaggctgttg accgtggcgt ccacatgggc cggaacgcag
     4321 tatcctccat ggtttcgggc cacttcgaca gcggtgctga ggtcccaaat ggaggaattc
     4381 atcagcgctc taagttccat tcctaaaatg ttctcatttt cgtcaatgat aacttgatca
     4441 ccaaaaaatt ccgggtcgtt gtccagcggc agtagcgagg cataaagctc gccgccaaag
     4501 gatttggccg ctgccgggtc gtcaaaatag accagcaaat ggatttcctc ggcggtctgc
     4561 atttccacgc cccaggtgaa agccagcccc tccctttctg cgacggcgga ataagcgggg
     4621 cagttggcaa gggaattgtg gtccgtgatg gccatccagt caatcccgca actcttggcc
     4681 cgcgcaacga cttcccgggg cgacatttcc aggcccccgc agggtgaaag cacggaatgg
     4741 atgtgcaggt ccgccttaaa ccatcgcatc ggctagatta actgatgcag catcccgtaa
     4801 aggattcccg ctaggctgaa ggtgtccagc gggctggata ccagacagat tccctcgctg
     4861 gcggcttttt ccaccaccat ctcgtccggt gtgttgcctt tggcgaaaac tatcgccgga
     4921 attccggtga gcgaagccac ggccacggtg ttcaggtgct tc
//
LOCUS       DAOOWR010000107         4902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17370_length_4902_cov_2.889004, whole genome shotgun sequence.
ACCESSION   DAOOWR010000107 DAOOWR010000000
VERSION     DAOOWR010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17370_length_4902_cov_2.889004"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLB85_03675"
     CDS             <1..460
                     /locus_tag="PLB85_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72459.1"
                     /translation="RIFAMASLIYKTAEQGLMEFFLERVLKDKADGKKVFKTFAGIFA
                     DAVMPGTEKLPRPGEGLIANIYVNIFLPVSYMYCDKHSDNETRERIIRYYSGFPALQE
                     NHVTRFMARYLSPTHQRAVNGKTLMQQGLMEIFHRYCRHHLCAECQADKP"
     gene            603..1379
                     /locus_tag="PLB85_03680"
     CDS             603..1379
                     /locus_tag="PLB85_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72460.1"
                     /translation="MKRLLIVLLALVAALTGVSCNRDKNKLPDGTDKVSEYNAVPSFK
                     AVFLALDKVEPGDLDALKPTTFAPAPQDSLHSAFAWGALAADAQLAVARRNTNWLNSI
                     LVQLKTLAPVMHRQTNLVNKLEQSIKPMLAEGNWEQIKQLFYDLQTTTDKMLMEQNRW
                     NVYTLAALGSWTQSVNQIGSLIAKSYSTEKSLVLKSTAWESLADNLLLINSPELTQAY
                     TKTQELRDIMNSAGQNAFTLEQVTMIVAATDSIRQSLSTP"
     gene            1393..2502
                     /locus_tag="PLB85_03685"
     CDS             1393..2502
                     /locus_tag="PLB85_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72461.1"
                     /translation="MKNILTGLLLLLACALAATAEQPVFSVVDNPWDDGTSVLVKWNL
                     ASVADSLSLQKADSLGAYQEIFNEFTQSGQFIDSSLEIGKDYRYRLIAYNRASGDSLT
                     YLGSARTEAQWFDMNKLSLLILLVLICGAILYYIFSARRGKDLFIRRISGLDSMDEAI
                     GRATEKGKPILFVPGISDLGDIQTIAGLNILSHLAYKSAEYGTELLVPCRFSMVLSAA
                     REAVKEAYLKAGRPDAYREDMVFYVTDDQFGFVAGVDGITLREQPAANFFLGAFWAES
                     LILTETGFGTGAIQTAGTGQPHQIPFFVVSCDYTLIGEELYAASAYISRDPQQLGSLK
                     GHDFGKLLVILLVLSGVVMEIIGAGWFKKFFEGGN"
     gene            2505..3203
                     /locus_tag="PLB85_03690"
     CDS             2505..3203
                     /locus_tag="PLB85_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72462.1"
                     /translation="MKRNIPLIVAFVSGVIILIAGFIPRDPFPRIESTLIDWFMIISS
                     FAILLGQASLLRMNVTRVAEKAPNWQYHLAGLVSFGLMLVFGLLWGMEKTPGMLGQGQ
                     GIVEKLGMKPFDYLFENAFIPLSSTMFSLLAFYIASAAYRAFIIRNFESNLLMITAVI
                     VMLGRTSFAPMLTGWIPESLSWLRLPELTNFIMQYPNTAAQRAILISAALGIVGASLR
                     IILGIERSYLGGGK"
     gene            3200..4072
                     /locus_tag="PLB85_03695"
     CDS             3200..4072
                     /locus_tag="PLB85_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72463.1"
                     /translation="MKKPDKTVIERRIVFLLLVLAVALPIILPLGLKTQSTPLTEQVY
                     DLVNSLPAGAPVIISFDYDPSTITELQPMAEAVIEHAWRKGHKVIATAMWPQGPQVAE
                     MAFNVVQARMEEKDPNARKLMYGVDYVNLGYKPGGMVTIQGMGRSMEAIFPTDITGLK
                     YNKIPMLSPVRTLKDIRYVISLSAGDPGLRDWIMTANGKFNIPVAGGTTAVSAPGFLP
                     YVNDQKQLHGLMGGLKAAAEYELLLGYEGTASLGMNPQSVAHVLILALILFGNIRAWK
                     KRKADKLAQEAKHG"
     gene            4065..4703
                     /locus_tag="PLB85_03700"
     CDS             4065..4703
                     /locus_tag="PLB85_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72464.1"
                     /translation="MANIMTTFGIWLAAFFTLSIFSFLYKDNPFYKFAEQVFVGLSAA
                     YWLVYTIYNILIPNLFNKLFTDFAGNWVLLIPMLLGIMMLLRMFPKLQWLSLYPLALM
                     VGTSAAMSMLLSFKSDFVEQMAATMINPFKFSSPVDVVGSIMLIVGTLCGVFFFYFSK
                     KQEGFYKVPSKIGIWFLMISFGASFGYTVMARISLLIGRLDFLLRDWLHIIK"
     gene            4716..>4902
                     /locus_tag="PLB85_03705"
     CDS             4716..>4902
                     /locus_tag="PLB85_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72465.1"
                     /translation="MQQLQHRFIQSAKKYGRKIAVHDQATGKDFTYDRLLIASLIMKG
                     SLARIRGRYVGILLPTSV"
BASE COUNT         1213 a         1320 c         1253 g         1116 t
ORIGIN      
        1 ccgcatcttc gccatggcca gcttgatcta caaaacagcg gagcaaggtt tgatggagtt
       61 tttcctggaa agggtgctga aggataaagc agatggcaaa aaagtcttta agacctttgc
      121 cgggatcttt gccgacgccg tcatgccagg cacggaaaaa ctgccccgcc cgggcgaagg
      181 tctgatagcc aacatctacg taaacatctt cctcccggtg agttacatgt attgcgacaa
      241 gcactccgac aacgagaccc gggaacggat catccgctac tacagcgggt ttccagccct
      301 gcaggaaaac catgtgacgc gtttcatggc aaggtatctc agccccacgc accagcgggc
      361 cgtgaacgga aaaaccctga tgcagcaagg gctgatggaa atatttcacc gctattgccg
      421 gcatcacctc tgcgccgaat gccaggcaga caaaccctga tgccaagaga tgcagtaggt
      481 ttattgtttt ttgaaataca agcggaacac gctaaggttg ggcacaccct acatgaaccg
      541 cgaaaaccat gccggacaaa taaatcgccg gcaataacct ttattgaact gaggaacttg
      601 agatgaagag actgctaatc gttttactgg ctttggtggc cgcactaacc ggagtttcct
      661 gcaacaggga taaaaacaag ctgcccgacg gcactgacaa ggtttctgaa tacaacgccg
      721 tcccatcttt caaggcagta tttctggctt tggacaaagt ggaaccaggc gatctggacg
      781 cgctaaaacc taccactttt gcccccgccc cgcaagactc gctgcattcc gcctttgcct
      841 ggggcgcctt ggcggcggac gcgcaactgg ctgtggccag aagaaataca aactggctta
      901 attccatcct cgtccaactg aaaaccctag cccctgtgat gcataggcag acgaacctgg
      961 tgaacaagct cgagcaaagc attaaaccaa tgctggcaga gggtaactgg gaacagataa
     1021 aacagctttt ttacgacctg caaacaacca cggacaaaat gctgatggaa caaaaccgct
     1081 ggaacgtcta cacgctggct gcattgggta gctggaccca gtccgtgaat cagatcggca
     1141 gcctgatagc caaaagttac agcaccgaga aatccctggt gcttaaatca accgcttggg
     1201 aaagcctggc agacaatctg cttttgataa attcccccga actcacccaa gcttacacca
     1261 agactcagga actgcgggac ataatgaatt ccgctgggca aaatgccttt actctggagc
     1321 aggttacaat gatcgtggca gccactgatt ccatcaggca aagcttaagc actccctgat
     1381 aaggagatgg acatgaaaaa tatactaaca ggcctactgt tgctgctggc ctgcgcgctg
     1441 gctgcaacag cggaacaacc tgttttctcg gttgtggaca atccttggga cgacggcact
     1501 tcggtgctgg taaaatggaa cctggcctcc gttgcggatt ccctgagcct gcaaaaagcc
     1561 gattccctgg gagcctatca agaaatattc aacgaattca cgcagtcagg gcaattcatc
     1621 gattccagcc tggagatcgg aaaggattac cgctaccgcc tgattgctta caacagggca
     1681 tctggagaca gcctgaccta tctgggctct gcgcgcaccg aggcacaatg gttcgacatg
     1741 aacaagcttt ccctgctgat cctattggtg ttgatctgcg gcgccatcct ctactacatc
     1801 ttttctgcaa ggcgaggcaa ggacctgttc atccgccgca tcagcgggct ggacagcatg
     1861 gacgaagcta taggccgcgc taccgaaaag ggaaagccga tcctctttgt gcccggaatc
     1921 agcgacttgg gcgacatcca gaccatcgct gggctgaata ttctcagcca cctggcctat
     1981 aaatccgcgg aatacggcac cgaactgttg gttccatgcc gcttttccat ggtgctctcc
     2041 gcagcaaggg aagctgtgaa ggaagcttat ctgaaagccg ggagaccgga tgcataccgt
     2101 gaggacatgg ttttttacgt gaccgatgac caattcggct tcgtagcagg cgtggacggc
     2161 atcacgctgc gcgaacaacc agcggcgaac tttttccttg gcgcgttctg ggctgaatca
     2221 ctaatcctta cggagaccgg ttttggcacc ggggccatcc agaccgccgg cactggccaa
     2281 ccgcaccaga tccccttctt cgttgtttcc tgtgactata cactgattgg agaggaactt
     2341 tacgccgcct cagcttacat ttcccgcgac ccccaacaac tcggcagcct gaaaggacac
     2401 gatttcggca agctgctggt gatcctgctg gtgttaagcg gtgtggtgat ggagataatc
     2461 ggggcaggct ggttcaagaa attctttgaa ggtgggaact gaacatgaaa cgaaacatac
     2521 ctctgatcgt tgccttcgtc tccggagtga taatcctcat tgcaggattc atcccgaggg
     2581 accccttccc cagaattgaa tccaccctca tcgactggtt catgatcatc tccagtttcg
     2641 ccatcctgtt ggggcaggca agtttgctgc gcatgaacgt gacccgggtg gcggaaaaag
     2701 cacctaactg gcaatatcac ctggctggac tggtcagttt tggcctgatg ctggtcttcg
     2761 gtctgctctg gggcatggaa aagacgcccg ggatgttagg tcagggccag ggaatcgttg
     2821 aaaaactggg catgaagcca ttcgattatc tcttcgaaaa cgccttcatc ccgctctcat
     2881 caaccatgtt ttcgctgttg gctttctaca tcgcctctgc ggcttaccgg gccttcatca
     2941 tccgcaattt cgaatcaaac ctgctgatga tcacagctgt gatagtgatg ctgggccgca
     3001 ccagtttcgc acccatgctc acgggctgga ttccggaatc actaagctgg ttgagattgc
     3061 ctgagcttac aaacttcatc atgcagtatc ccaacacagc agcacaaaga gcgatcctga
     3121 tcagtgccgc gctgggcatt gtgggagctt cgctgcgcat tattctgggc atagaaaggt
     3181 cctatttggg aggcggcaaa tgaagaagcc cgataaaacc gtcatcgaac gcaggatcgt
     3241 atttctcttg ctggtgctgg ctgtcgcttt gccgatcata cttccactgg gcctcaaaac
     3301 ccagagcacg cccctcacag agcaggttta cgatcttgtg aattccctgc ctgctggcgc
     3361 tcccgtgatc atttccttcg attatgaccc ctccaccatc accgaattgc agcccatggc
     3421 cgaagctgta atagagcacg cctggagaaa aggccacaag gtcatcgcca ccgcgatgtg
     3481 gcctcaagga ccgcaggtgg cggagatggc tttcaacgtt gttcaggcca gaatggaaga
     3541 gaaagatcca aatgccagaa aactcatgta tggtgtggat tacgtcaacc tgggctacaa
     3601 accgggcggc atggtgacga tacagggaat ggggcgcagc atggaagcca tcttccccac
     3661 cgacatcaca ggcttaaaat acaacaagat cccgatgctg agtcctgtcc gcactttgaa
     3721 ggacatccgc tacgtaatct cgctttccgc tggcgatccc ggcttgcggg actggatcat
     3781 gaccgccaac ggcaaattca acataccggt cgcaggaggc actactgccg tgagtgcacc
     3841 gggtttctta ccctatgtga acgaccagaa acagctgcat ggactgatgg gcggcttgaa
     3901 agcggcagcg gaatatgaac tgttgctggg atatgaagga accgcaagcc tgggcatgaa
     3961 tccccagtcc gtggctcacg ttttgatcct ggcgctcatt ctcttcggaa acatccgggc
     4021 ctggaaaaaa cgcaaggctg acaaactcgc tcaggaggcc aagcatggct aatataatga
     4081 ccacttttgg aatctggctg gcggctttct tcacgctcag catcttcagt ttcctatata
     4141 aggacaaccc cttctacaaa ttcgcggagc aggtcttcgt gggcctctca gcggcatact
     4201 ggctggttta caccatctac aacatcctca tccccaatct cttcaacaaa ctgttcactg
     4261 attttgccgg caattgggtg ctattgattc cgatgctgct gggcatcatg atgctgctga
     4321 ggatgttccc aaaactgcaa tggctcagcc tttacccact ggcgctgatg gtgggaactt
     4381 ctgccgcgat gtcgatgctg ctcagcttca agtccgactt tgtggaacag atggctgcca
     4441 cgatgatcaa tcctttcaag ttctcctcac cggttgacgt ggtgggcagc atcatgctga
     4501 tcgtaggaac gctctgcggt gtgtttttct tctatttcag caaaaagcag gaaggctttt
     4561 acaaagtgcc ttccaagata ggaatctggt ttttgatgat aagcttcggg gcttcgtttg
     4621 gctacaccgt aatggcccga atctcgctgc ttataggcag actggatttc ctgctgcgcg
     4681 attggctgca tataatcaaa tagaaaggag tttatatgca gcaattgcaa caccgcttta
     4741 tccaatcggc caagaaatac ggcaggaaaa tcgccgtcca tgatcaggcg acgggaaagg
     4801 atttcaccta tgacagatta ctgatcgcct ctctgataat gaaaggcagt ctggcaagga
     4861 tcaggggaag atatgttggt atcctgcttc ccacctcagt ag
//
LOCUS       DAOOWR010000108         4893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17399_length_4893_cov_4.204630, whole genome shotgun sequence.
ACCESSION   DAOOWR010000108 DAOOWR010000000
VERSION     DAOOWR010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17399_length_4893_cov_4.204630"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLB85_03710"
     CDS             <1..385
                     /locus_tag="PLB85_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72466.1"
                     /translation="PSLSRLAEVLLGSHDFSSLSRDNPLVPNHICDIQRLEISEERGI
                     IRFDLTADRFLHNMVRRIVGTLVNLDHGGLGPDVLQAILDEANPRQKLVTTAPAQGLY
                     LTGVRYPNLQLDESAPTDELLYFLR"
     gene            388..1650
                     /gene="hisS"
                     /locus_tag="PLB85_03715"
     CDS             388..1650
                     /gene="hisS"
                     /locus_tag="PLB85_03715"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU72467.1"
                     /translation="MQYKRPRGTYDILPEQSYKWHKVTAAFRELAAAFGYEEISTPAF
                     EQAALFERSSGESSDVVRKEMYRFTDQKGREFALRPEGTAPVARAYLENRLDIGSAAR
                     KLYYIGPMFRYDRPQAGRYRQHQQYGVEFIGSNHPYYDAEVISILWLYLSGMGLTNLR
                     LELNSVGCADCSGEYDKALQAYFRPHLAELCPDCQVRIDLKPRRLLDCKVMTCKEIAK
                     DAPSQLDYLDEPCRVHFADTQNHLELMKIPFTINPRIVRGLDYYTNTAFEIVSMGLGA
                     QNSLAGGGRYNGLLEQIGGKSIPAIGFAGGFERLLLALEEENVSLGKAPVPDVFVIPM
                     GAAAFEFILPYLNELRKAGVFAELDPDKHSFKAQLKAADNRQARYALIVGETELAQKS
                     AVLKDLNSGEQKEYPLEPLSELIDAIRR"
     gene            complement(2018..2713)
                     /locus_tag="PLB85_03720"
     CDS             complement(2018..2713)
                     /locus_tag="PLB85_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU72468.1"
                     /translation="MNDKKAERVIDTFTGPLERKILCKLANALPEKVLPDHLTVLAFV
                     GTLISAAAYLLSAQSRCWLFLASFGFVLNWFGDSLDGTLARVRHIERERYGYFVDHLA
                     DALTTVIICLSFGLSPLMRFDIAMMIAIGYLLMNVYVHVSAYTQGEFRISYMKFGPTE
                     IRLAIILFNTVMFFWDGQVLEYRGNHLTIMDLGGLVFSLAFVVIFIVSFIKTALKLDK
                     LDRAKKRDEGKQE"
     gene            complement(2710..3192)
                     /locus_tag="PLB85_03725"
     CDS             complement(2710..3192)
                     /locus_tag="PLB85_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU72469.1"
                     /translation="MKTAKLITGLKRINLRKLISFHLIAWVGTLVNLAVLWLTHGVLK
                     IPLMFAGAIAIEVAIIHNFTGHYFITWKDRVSRSPKSYFLLLLRYNIVTASIDFVINL
                     GILWLLTHFLGVHYMVANIAGQILGPLFKLLANEYLVFPKLKHKKTSDSVVKDIREKA
                     "
     gene            complement(3405..4757)
                     /locus_tag="PLB85_03730"
     CDS             complement(3405..4757)
                     /locus_tag="PLB85_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOU72470.1"
                     /translation="MIKPQVFRQYDIRGIVDEELNEESYYLIGKGFGSYLRQKGLRTI
                     ALGGDARLTTPKLKEAFIKGALETGCHITDIGLVATPVLYFAIWKMKTDGGAMVTASH
                     NPSQYNGCKLNLGLGSVYAEELQKVLKIIQAGDFASGEGSYVKDDSVNDAYIDYITAN
                     IHLERPVKVVIDGGNGMGGPYLPEILDRLGCELIEMYCDPDGTFPNHHPDPTVAKNME
                     DLARAVVAAEYELGIGLDGDADRIGVVDEKGKMLFGDQILNILARDYLKTNQGKTIIA
                     DVKCSKNLFDDIRARGGVPMMYKTGHANIKMKMKEIGVDFAGEMSGHVFLGDRYLGFD
                     DAIYVSCRLIEIVSKTDAPVSSYLADQPKMHNTPEMHIKCADERKFEVVAKVCEEFKA
                     EGYDVNDIDGARITFNDGWGLVRASNTTPVLVTRYEAESEDRMREIQALIEGKINKYI
                     "
BASE COUNT         1055 a         1359 c         1340 g         1139 t
ORIGIN      
        1 gcccagcctt tcccggctgg cagaagtgct gctcggctcc cacgactttt cctctctcag
       61 ccgcgacaat cctttggtgc ccaaccatat ctgcgacatc caaaggcttg aaatctccga
      121 ggagcggggc atcatccgct ttgacctcac cgcggacaga ttcttgcaca acatggtgcg
      181 ccgcatcgtt ggcaccctgg tcaatctgga tcacggcggc ctgggccccg acgttctgca
      241 agccatcctg gacgaggcca acccgcgcca gaaactggtt acaaccgctc ccgcccaggg
      301 tttgtacctc accggcgtgc gctatccgaa tttacagctt gacgaatccg cgcccactga
      361 cgaattgctt tatttcttga ggtgatcatg caatataaga gaccccgtgg aacctacgac
      421 attctgccgg agcaaagcta taaatggcac aaagttaccg ccgcttttag agagcttgcc
      481 gccgcctttg gctatgagga gataagcact cccgccttcg agcaggcagc gctgttcgaa
      541 cgctcatccg gagagagttc cgacgttgtt agaaaagaaa tgtaccgctt tacggaccag
      601 aaaggccgcg aattcgcgct gcgtcctgaa ggaaccgctc cagtggccag ggcttacctg
      661 gaaaaccgcc tggacatcgg ctccgcggcg cgcaagctgt attacatcgg ccccatgttc
      721 cgttacgacc gcccccaggc agggcgttac cgccagcacc agcagtacgg cgtcgagttc
      781 atcggcagca atcatcccta ttatgacgct gaagtgatct ccatcctctg gctctacctc
      841 agcggcatgg gcctcaccaa tctgcggctg gaactgaaca gcgtgggctg tgccgactgc
      901 tccggagaat atgacaaagc gctgcaggca tacttccgtc cccatttggc ggaactctgt
      961 ccggactgtc aggtgcggat agacctcaaa ccccgccgcc tgctggattg caaggtgatg
     1021 acctgcaaag agatagccaa ggacgcacct tcccaactgg actatctgga cgagccctgc
     1081 agggtccact tcgccgacac ccagaaccat ttggaactga tgaagatacc atttaccatc
     1141 aatccccgca tcgtgcgcgg cctggattat tacaccaaca ctgccttcga gatcgtttca
     1201 atgggcctgg gcgcgcagaa ttccctggcc gggggcggac gctacaatgg cctgctggaa
     1261 caaatcggcg gcaaaagcat acccgccatc ggtttcgccg gtggcttcga acgcctgctg
     1321 ctggctctgg aagaggaaaa cgtgtccctg gggaaagctc ctgttcccga tgtttttgtg
     1381 attccgatgg gtgccgcggc attcgagttc attttgcctt atctgaatga gttacgcaag
     1441 gctggcgttt ttgccgagct ggatccggac aagcattcct tcaaagcgca actgaaagcc
     1501 gccgacaacc gccaggcgcg ctatgccctg atcgtcggcg aaaccgaact cgcccaaaaa
     1561 agcgccgttc tcaaggacct gaacagcggc gaacaaaaag aatatcccct ggagccgctc
     1621 tccgagctca tcgacgccat ccggcgctga cccccttttc cgcaaaaccg gtttattagg
     1681 ctctatatct aatcgggcag ataatgccct gggacggcct gacgtccagc aaacacagtg
     1741 agcatggacg tcaggaatat ttgctgccgt cgatgctcgt tcctcgctcg acgccagcat
     1801 ttagcttggg agaagcgaca tggtctggcc gttaaggccc ttccctgtga cccggacgcg
     1861 cctcccgcat catgtccgca ttcgatgcga ggatcagacg ggaggcatta aagagggata
     1921 gaatcgggcg ttaaggacgc ggctggcagg atacaatggc tgtatagccc gcagagcggc
     1981 aggatgatgg tagaatcttt ccgcggattg tccggcctta ttcttgtttg ccttcgtccc
     2041 gctttttggc ccggtcgagc ttatccagct tgagagccgt tttgatgaag cttacgatga
     2101 atatgaccac aaacgccagg ctgaaaacca atcctcccag gtccatgatg gtcaggtggt
     2161 tgccccggta ttccagcact tgaccgtccc agaagaacat gacggtattg aagaggatga
     2221 tggccagcct gatctcggtg gggccgaact tcatgtagga gatgcggaat tcgccttgag
     2281 tgtaggcgga aacgtgcaca tagacattca tcagcagata gccgatggct atcatcatcg
     2341 cgatgtcgaa gcgcatcagg ggtgacaggc cgaaagacag gcagattatc accgtggtca
     2401 gggcgtcggc cagatggtcc acaaagtagc cataacgctc ccgctcgata tgcctaaccc
     2461 gggccagggt gccgtccagg ctgtcgccaa accagttcag cacgaagccg aaactggcca
     2521 gaaacagcca gcagcgtgat tgcgcactca gcagataggc agccgcgctg atcagcgtgc
     2581 ctacaaacgc caaaaccgtg agatggtcgg gaagaacctt ctccggaagg gcgttggcca
     2641 gcttgcatag aatttttctt tccaggggac cggtgaatgt gtctatcacc ctctcagctt
     2701 ttttatcgtt catgctttct cccttatatc cttgactaca gaatcggagg tctttttgtg
     2761 ttttaatttg ggaaacacca gatattcgtt ggccagcagc ttgaatagcg gccccaaaat
     2821 ctgtcccgcg atgttggcaa ccatgtaatg gacgcccagg aaatgggtca gcagccataa
     2881 aatgcccaga tttatcacga aatctatgga cgcggtgacg atgttatagc gcagcagcaa
     2941 gaggaaataa ctcttagggg acctgctgac gcggtctttc caggtgatga agtaatgccc
     3001 tgtgaagttg tggatgatgg ccacttcgat cgcgatggcg cccgcgaaca tcagcggtat
     3061 cttcaacacg ccatgcgtaa gccacagaac ggcaaggttt accagcgtcc ccacccaggc
     3121 gatcagatgg aagctgatca gctttctcag gttgatcctc ttcaatcccg taataagttt
     3181 ggctgttttc atcaataatt ccttgcgcgt tgcgtgtaac ctttatatcg ggtatttcac
     3241 taaataactt tcagtaggtc gtctgataat tccaagcttg ttttctgtaa aggaatttct
     3301 ttgggcacta tcaaatgtga tgtccaattc gtcacggaaa atggactgaa tgacaagaaa
     3361 cccggaaccg cgtggctccg ggttgtagta gctttatgcc tgcgtcagat atacttgttg
     3421 attttccctt cgatcagggc ctggatttcg cgcatgcggt cctcggattc ggcttcgtaa
     3481 cgggttacca ggacaggagt tgtgttggag gcgcgaacca gtccccagcc gtcattgaag
     3541 gtgatgcgcg cgccgtcgat gtcgttcacg tcgtaaccct cggccttgaa ttcctcacag
     3601 accttggcca ccacctcaaa tttccgctcg tcggcgcatt tgatgtgcat ttcgggagtg
     3661 ttgtgcattt tgggttggtc agccaggtaa gagcttacgg gggcgtcggt tttggaaacg
     3721 atttcgatca ggcggcagct cacatagatg gcgtcatcaa agcccagata gcggtctccc
     3781 agaaagacgt ggccgctcat ttcgccggcg aaatccaccc cgatttcctt catcttcatc
     3841 ttgatgttgg cgtgaccggt tttatacatc atgggcacgc ctccgcgggc gcggatgtcg
     3901 tcgaagaggt tcttggagca tttcacgtcg gcgataatgg tcttgccctg gttagttttc
     3961 aggtagtcgc gggccaggat gttgaggatc tggtcgccga agagcatttt gcctttttcg
     4021 tccaccacgc cgatgcggtc cgcgtcgccg tccaggccga tgcccagttc gtattcagcc
     4081 gccacgacgg cacgggcaag gtcctccatg ttcttggcca cggtggggtc cggatggtgg
     4141 ttggggaaag tgccgtcggg atcgcaatac atttcaatga gttcacagcc gaggcggtca
     4201 aggatttccg gcaggtaggg gccgcccatt ccgttgccgc cgtcgatcac gaccttcacc
     4261 ggccgttcca gatggatgtt ggcggtgatg taatcaatgt aagcgtcatt gaccgaatcg
     4321 tcttttacat agctgccctc cccgctggca aaatccccgg cctggatgat cttcaacacc
     4381 ttctgcagtt cctccgcgta aacggaaccc aggcccagat tgagtttgca gccgttgtat
     4441 tgggaggggt tgtggctggc ggtgaccatg gctccaccat cggttttcat tttccagatt
     4501 gcgaaataga gcacgggggt ggccacgagg ccgatatcgg tgatatggca gccggtttcc
     4561 agggctccct ttataaatgc ttccttcagc ttgggtgtgg taagccgggc gtcgccgccc
     4621 agggcgatgg tccgaaggcc tttctggcgc agatagctgc caaagccttt gccgatcaaa
     4681 tagtatgatt cttcgttcag ttcttcgtca acaatgccgc gaatgtcgta ttggcggaat
     4741 acttggggtt taatcatgat taacctcttt atctgattca ataatgtctt tcaggatggg
     4801 cagcatgtct gccaaagccc tggcccggtg ggaaagcgcg ttcttttcgg cgtcggtcat
     4861 ctcggcgtag ctgcgcggtc ccggatcggg aat
//
LOCUS       DAOOWR010000109         4873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17483_length_4873_cov_3.707555, whole genome shotgun sequence.
ACCESSION   DAOOWR010000109 DAOOWR010000000
VERSION     DAOOWR010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17483_length_4873_cov_3.707555"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLB85_03735"
     CDS             <1..208
                     /locus_tag="PLB85_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOU72471.1"
                     /translation="YTEADLDSTRTYIRTVLKSELLRTVYGDLEAYKTTIQLDRQFLE
                     AVALLNRFRSMDELFAHAAAQNKN"
     gene            356..694
                     /locus_tag="PLB85_03740"
     CDS             356..694
                     /locus_tag="PLB85_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72472.1"
                     /translation="MKNLLFIFVLLALSFGAFGHAATELQASYTAATQTLKLSFDHSV
                     KNPQDHFIQSIEIRHNGTMIISQVASAQDSTTGGEYIYKIPNLKKNDKIDITLICNKG
                     GRKSASMILK"
     gene            complement(1002..1994)
                     /gene="trpS"
                     /locus_tag="PLB85_03745"
     CDS             complement(1002..1994)
                     /gene="trpS"
                     /locus_tag="PLB85_03745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU72473.1"
                     /translation="MKVALTGIKPTGIPHIGNYLGAIRPALELSRSCEARYFIADYHA
                     LNACKDPAAIRAMTLEIAAAWLASGLDPDKVLFYKQSLVPQTFELLTILLAFTPKGLM
                     NRAHAYKAILQANAASGRDPDDGVNMGLFTYPVLMAADILLFDTDLVPVGNDQFQHVE
                     MAVDIAQSFNFTYGQECLRLPQPVATEHSKTLLGLDGRKMSKSYGNTIPMFASEKELR
                     KLVMRIVTNSQGVEEPKDPETCTVFALYRNFASPEQVESLRQRYLAGGMGWGHAKQEL
                     FEVMNVALTPLRERYLELMSEPEKITAILEAGSAKARELAAAKIRQLRSVIGMD"
     gene            complement(1991..3301)
                     /gene="rimO"
                     /locus_tag="PLB85_03750"
     CDS             complement(1991..3301)
                     /gene="rimO"
                     /locus_tag="PLB85_03750"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOU72474.1"
                     /translation="MSKYFIESLGCAKNLVDSEVFAAILERAGYEAADYPEEADLVLV
                     NTCSFLVDSLRELDLVLSDLASLKQEGQFDRLLVTGCLMNRGLEDFQSLFPEVDAWIR
                     LKDFAALERWLRLEPVSDLPRTAIQGGFHRYLRISDGCSNNCSYCAIPSIRGSMASVP
                     IEELMREAEALAADPEEGWQELVVIAQDTANYGLDIYGRKALPELLEKLCALPQYSWV
                     RVMYMHPDHFETAWLELWKRQPKLLPYFEIPVQHSEDRILKAMNRKKGRRELEELFGT
                     ILQELPQAVLRTTVMSGFPGETRAEALALKKFVAGIPFLHLGVFSYSREAGTPAFSLE
                     GQVPPRTAERRMNALLAQQANRREELLERYVGQSVEMLVEEAEGENEGDAWIGRAWFQ
                     APEVDGVTYIEGSGLQPGQIIRAEVTNTVDPDLFAAVYPKENEQ"
     gene            complement(3298..3774)
                     /locus_tag="PLB85_03755"
     CDS             complement(3298..3774)
                     /locus_tag="PLB85_03755"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72475.1"
                     /translation="MVKDLNGHSKEEFLKLVEEKFEITPIKEAGNFHPGKTHEIGMYL
                     DRCWYRLVPKAGTWDAANPVASLDVSILQDNLLHPILGIGDPRRDTRIDFVGGIRGLD
                     ELVRRVDSGREVVAFAMYPTSMDELLSIADAGMIMPPKSTWFEPKLRSGLFIHPLT"
     gene            complement(3768..4547)
                     /locus_tag="PLB85_03760"
     CDS             complement(3768..4547)
                     /locus_tag="PLB85_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOU72476.1"
                     /translation="MAVFKPFRALRPVPKYAADIASLPYDVMDSDEARIEVKKHPLSY
                     IHVEKPEVDLPVGTDLYDPAVYAKAKENLDNYSRQGYMMQDPKPYFYIYCQTMDGREQ
                     NGLVGLASVDEYMDGKIKKHEFTRADKEADRIRHVDTCDAHPSPVFFTYRHQDAIDTV
                     VEKVKQAKDPVYDFVSDDGIGHSLWLVDEPADIAAIQDAFAKMDCLYVADGHHRTASA
                     AKVGLLRREKYPDYTGEEEFNFFMTVIFPDNQLRYLITTAW"
     gene            complement(4573..>4873)
                     /locus_tag="PLB85_03765"
     CDS             complement(4573..>4873)
                     /locus_tag="PLB85_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOU72477.1"
                     /translation="PAMNGGPAIKWNASHKYTTTVASSTVFAQNAKAAGVNCQKFMMH
                     SDLLCGSTVGSMLAAKLGISTVDVGNPLWAMHSIRETAGVDDHLDMIKVLSHYYH"
BASE COUNT         1027 a         1408 c         1367 g         1071 t
ORIGIN      
        1 atacaccgaa gctgatctgg acagcacccg cacctacatc cgcactgtgc tcaaaagtga
       61 actgctgcgc accgtttatg gagacctcga ggcttataaa accaccatcc agctggacag
      121 gcagttcctg gaagccgtgg ctcttttgaa caggttccgc tccatggacg agcttttcgc
      181 ccacgccgcg gcgcaaaata aaaactaatg caggataacg cctccaacca ggtataacct
      241 atggagttaa atagaatgat acatactgga agggcctggc ccaagctgat tgccagccag
      301 gcccttccat cttcaagaca aacactattc agaaccttat aaggagatca ttataatgaa
      361 aaacctcctc tttatctttg tccttcttgc cctcagtttc ggtgctttcg gccacgccgc
      421 aaccgagtta caggcctcat acaccgctgc cacccaaacc ctgaaactaa gcttcgacca
      481 cagtgtcaag aaccctcagg accacttcat ccaaagcatt gaaatccggc acaacggcac
      541 catgatcatc tcccaggtgg ccagcgcgca ggattcaacc accggcggag agtacatcta
      601 caaaatcccc aacctgaaaa agaatgacaa gatcgatata acgctcatct gcaacaaagg
      661 tggcagaaaa tcagcttcaa tgatcttgaa ataaacaatt cccatccatc tcctcattca
      721 taatcccgga cggcctgccc aaggccgtct ttttttggat ccctttcaaa gagagccaga
      781 tatacccgcc gcgctgtcct gccagtcagg ccctgagcgc ttgaggccat ccctctccca
      841 tgccactcgc caaatactcg caccaaatac ggccatcatg cgggaggcgc atccgggaca
      901 caggtcaggg ccttaacggc cagaggactc ccgacaaggc tttcaaaaaa aaccggggcg
      961 gcagtcgccc cggctaaaca tctcattgtg gagcaataag ttcaatccat cccgattacg
     1021 gagcgcaact gcctgatctt ggcggcggcg agttccctgg ccttggctga gccggcttcc
     1081 agaatggcgg tgatcttttc gggctcggac atcagttcga ggtagcgctc gcgcaggggt
     1141 gtgagggcaa cgttcatcac ttcaaacagt tcctgtttgg catgccccca gcccatgcct
     1201 ccggccagat agcgctggcg cagactttcc acctgctccg ggctggcgaa attgcggtac
     1261 agggcaaaga ctgtgcaggt ttcggggtcc ttgggctctt ccactccctg ggaattggtc
     1321 acgatgcgca tcaccagctt gcgcagttcc ttttcggaag cgaacatggg aatggtgttg
     1381 ccgtagcttt tggacatctt gcgcccgtca agccccaaca gggttttgct gtgctcggtg
     1441 gcgacgggct gaggcaggcg cagacactcc tgtccatagg tgaaattgaa gctctgggct
     1501 atatccacgg ccatttccac gtgctggaac tggtcatttc ccaccggcac caggtcggtg
     1561 tcgaagagca ggatgtcggc cgccatcagc acgggatagg tgaagagccc catgttcacg
     1621 ccgtcgtcgg gatcgcgtcc gctggcggca ttggcctgga ggatggcttt gtaggcgtgg
     1681 gcgcggttca tcaggccctt gggtgtgaaa gccagcagaa tggtcagcag ctcaaaggtc
     1741 tggggcacaa gggattgctt gtagaacagc accttgtccg gatcaagccc cgaagccagc
     1801 caggcggccg cgatctccag agtcatggcg cgaatggcgg cggggtcctt gcaggcattg
     1861 agggcgtgat agtcggcgat gaaatagcgc gcttcacaac ttcgcgaaag ttccagagcc
     1921 gggcggatgg cacccaagta attgcctatg tgggggatac cggtcggctt gatgccggtg
     1981 agggctactt tcattgttca ttctcctttg ggtaaactgc tgcaaaaagg tcggggtcca
     2041 cggtgttggt gacctctgcg cggattatct ggccaggctg caggccgctg ccttcgatgt
     2101 aggtgacgcc atccacctcc ggcgcttgaa accaggcccg gccgatccag gcgtcccctt
     2161 cgttttcgcc ttccgcttcc tccaccagca tttccacgct ctggcccacg tatctttcca
     2221 gaagctcttc acgacggttc gcctgctggg ccagcaaagc gttcatcctc ctctccgcgg
     2281 ttctgggcgg aacctgccct tccagcgaaa aggccggggt gcccgcctca cgggaatagc
     2341 tgaacacgcc aaggtgaaga aagggaattc cggccacaaa ctttttaaga gccagggctt
     2401 cagccctggt ctcgccggga aagccgctca tcacggtggt gcgaagcaca gcctggggca
     2461 gttcttgcag aattgtaccg aaaagctctt ccagctcccg gcgccccttt ttgcggttca
     2521 tggctttcag gatgcggtct tcgctgtgct ggacggggat ttcgaaataa ggcagcagct
     2581 tgggctggcg tttccagagt tccagccagg ccgtttcaaa gtggtccgga tgcatgtaca
     2641 tcacgcggac ccagctatac tgcggcaggg cgcagagctt ctccagcaat tccggcaagg
     2701 ctttgcgtcc gtagatgtcc agtccgtaat ttgctgtgtc ctgggcgatc accaccagtt
     2761 cctgccagcc ttcttcggga tccgctgcca aagcctccgc ttcccgcatc agttcttcga
     2821 tgggaacgct ggccatgctt ccgcggattg agggaatggc gcagtagctg caattgtttg
     2881 agcagccgtc gctgatgcgc agatagcggt gaaatccacc ctggatggcg gttctgggca
     2941 ggtcggaaac tggctccagg cgcagccagc gttccagcgc cgcgaagtcc ttcaatctga
     3001 tccaggcatc cacttcggga aagaggcttt ggaaatcctc caacccccgg ttcatcaaac
     3061 agcctgtcac cagcagccgg tcgaactgcc cctcctgttt cagagaggcc aggtcgctca
     3121 gcaccaggtc cagttcccgc agcgaatcca ccaggaaaga gcaggtgttc accagcacca
     3181 gatcagcctc ttcgggataa tcggcggctt cgtaacccgc cctttctagg atagcggcga
     3241 aaacctcact gtccaccagg tttttggcgc agcccaggct ttcgatgaag tatttgctca
     3301 cgtaagggga tggatgaaaa gtcccgaacg cagtttgggc tcgaaccagg tggatttggg
     3361 aggcatgatc atgcccgcgt cggcgatgct cagaagttcg tccatggagg tgggatacat
     3421 ggcgaaagcc accacttccc tgccgctgtc aacgcggcgg accagttcat cgaggccgcg
     3481 gatgccgcct acaaagtcga tccgggtgtc ccggcgggga tcgccgatgc ccaaaatggg
     3541 atgcagcaaa ttgtcctgca aaatagaaac gtccagcgag gccaccggat tggcggcgtc
     3601 ccaggtgcca gctttgggaa ccaggcggta ccagcagcgg tccagataca tgccgatctc
     3661 gtgggtcttg ccagggtgga aattgcccgc ttcctttatg ggggtgattt cgaatttttc
     3721 ttccaccagc ttcaggaact cctctttgga atggccgttc aggtctttca ccacgcggtt
     3781 gtaatcaaat atctaagctg gttgtctggg aaaatcacgg tcatgaagaa attgaactct
     3841 tcttctccgg tgtaatccgg atatttttcc cggcgcagaa ggcccacctt ggccgcgctg
     3901 gcggtgcggt gatgcccgtc agccacatag aggcaatcca ttttggcaaa agcgtcttgg
     3961 atggcggcga tgtcagcagg ctcatccacc agccagagcg aatggccgat gccgtcgtcg
     4021 gaaacgaagt cgtaaacagg gtctttggcc tgtttaactt tctccaccac agtgtcgata
     4081 gcatcctggt gacggtaggt gaaaaagacc ggtgaagggt gggcatcgca ggtgtcaacg
     4141 tggcggatgc ggtccgcttc tttgtcggcg cgggtaaatt cgtgcttctt gatcttgccg
     4201 tccatgtatt catcaaccga ggcgaggccc accaggccgt tctgctcgcg gccgtccatg
     4261 gtttggcagt aaatgtagaa ataaggcttg ggatcctgca tcatgtagcc ctggcggctg
     4321 tagttgtcca ggttctcctt cgctttggcg taaaccgcgg gatcgtaaag atcggtgccg
     4381 accggcaggt caacctccgg cttctccaca tggatatagc tgagcgggtg tttcttaacc
     4441 tcgatccggg cttcgtccga atccatcaca tcatagggca gcgaagctat gtccgccgcg
     4501 tatttgggta ccggacgcag ggcccggaag ggtttgaata ctgccatgag gcactcctat
     4561 cttattttta tattaatgat aatagtggga aaggaccttg atcatgtcca gatggtcgtc
     4621 cacgccagcg gtttcgcgaa tcgagtgcat ggcccagagc gggttgccga cgtccacggt
     4681 gctgatcccc agtttcgccg cgagcatcga gcccacggtg ctgccgcaga gcaggtcgct
     4741 gtgcatcatg aacttctggc agttcacgcc cgcggctttg gcgttctgcg cgaagaccgt
     4801 gctgctggca acggtggtgg tgtatttatg gctggcattc catttgatcg cgggcccgcc
     4861 gttcatcgcc ggc
//
LOCUS       DAOOWR010000110         4802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_17764_length_4802_cov_3.827049, whole genome shotgun sequence.
ACCESSION   DAOOWR010000110 DAOOWR010000000
VERSION     DAOOWR010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17764_length_4802_cov_3.827049"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="dinB"
                     /locus_tag="PLB85_03770"
     CDS             complement(<1..776)
                     /gene="dinB"
                     /locus_tag="PLB85_03770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOU72478.1"
                     /translation="MNKIIHIDMDAYFAAIEIREDPSLRGKCVIVGGNPNGRGVVSTC
                     SYEARKFGVHSGMSSHQAWKLCPQGIFLRCRFKLYKEVSEQIRAVFFSYTDLVETMSL
                     DEAYLDVTTNKAGLDDPVEIARRIKADILAATQLTCSAGVSFNKFLAKIGSELEKPDG
                     LSVITPEKAQEILFNLPIGKFYGVGRVTAARMKKMGIHTGEDLHRQELRDLTRVFGKM
                     GLYFYQVVRGIDNRAVITESDPKSISCENTFHTDLSSIEEL"
     gene            complement(1042..3006)
                     /locus_tag="PLB85_03775"
     CDS             complement(1042..3006)
                     /locus_tag="PLB85_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU72479.1"
                     /translation="MKKTAIILLLVGMAALAFGQIRSPGSRYAQEGLEYYQRGEYNRA
                     IREFLSADRSADGRVPEYHYWLGRLHIAVADTASAMRWFDKYLDSGDKQYQSQVDNYL
                     RIVQRQKKIFSKVNIRPLPDYVNSRNSDYGAILDPEGKYLYFTSLRPAKKGKENIWRS
                     EIFSSGFGQPELVDELSTDKNEAFGCFSADPQGAWIFGNYETNKRDGDIYFVPRTDKW
                     FQPQNISQLNSSQVETHPMVFRDRLLFFASSREGGYGGTDIYVSEKIGGIWTQPQNLG
                     PMINTPENEQTPFLDWDGRTLYFSSRGHPGFGGYDLFKAYRIGTTWQEWSLPENLGLP
                     VNSTRNDRYFYHVRGSNEGYISSDRQVSGFEKIYQINFSLARPTSYLVREADGSVRSV
                     DVEYVAPALRKPADEETIFARIAESLETIRQGLPKPGFVPAPLGREPQPTEALAEDVE
                     PIIIRVEGSVHDQFGEAVLTEVQISGETNGQRTVEVFNTNSLGNFLGTLPLADTYTAV
                     INTPGYLVHQQIIQDPRSLARVKLDIELQKLEKKASLSLPDILFENGSFELGSGATAE
                     LDLLVMSLLTNQGLKVKLSGHAWESGSAAENKDLSERRARAVADYLISKGIAKKRVSW
                     KAYGKTRTLDPQTEPSALGKSRRVEIMLQL"
     gene            complement(3023..3985)
                     /locus_tag="PLB85_03780"
     CDS             complement(3023..3985)
                     /locus_tag="PLB85_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72480.1"
                     /translation="MKTIKIMNYILPVVLLVLAATPIAAQEMNNSLLAYSRMGVGARV
                     IAMGEAGSTVTNDIVSGYWNPAGLTRMKDFELGTMVNLDMGYNRSHNYASFGNRFRFG
                     ALALSWVNANVLDIDGYDENENPTGTFSDSENNFTLSYANSIGDFSFGFGPKFYLSTL
                     EGESIYGYGVDFGAKYDINQYLEIGAMARDLYGEFDGATVPYQISAGLAAYPLKGLTL
                     AADAKMEKDEDPYFCFGAEYWTSIGRDPEADSKLSVVSVSEQSSWEETLSTLQTGLRL
                     GYNRGRLSAGTGIKLRNFQLDYVFRYNNHEVFGNDHILSLILRF"
     gene            complement(3996..>4802)
                     /locus_tag="PLB85_03785"
     CDS             complement(3996..>4802)
                     /locus_tag="PLB85_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72481.1"
                     /translation="FGAPVFVVISSPTEDSEIHYTTDGSEPTAESALYTEPVIVPNFA
                     QNMVISARAFKADWTPSPVASATYSIFSAPIDVRGFTYAGYIRVLWNLPDAGKGLDGF
                     NVYRRKLTESDFTKINTSLVNTQVDGNYYYDDYSIEMEVSYEYYVTAVYGGTESPASA
                     STVEYYQSQDLDISDASYAWPNPATEQTEIVVILNRNNNVTLTVSIYDFAGKKVQTIT
                     EPSQNSNQIRIPWNLKNSSGAKVGRGTYFARVVANDGVNRSEHVIKIAVK"
BASE COUNT          999 a         1266 c         1377 g         1160 t
ORIGIN      
        1 agttcttcga tagagcttag atcggtgtgg aaagtgtttt cgcagctgat ggatttgggg
       61 tcggactcgg tgatcacggc gcggttgtcg ataccccgca ccacctgata gaaatagagt
      121 cccattttgc caaagacgcg tgtcagatcg cgcagttcct ggcggtgcag gtcctctccg
      181 gtgtggatgc ccattttctt catcctggca gcggtgaccc ggccaacgcc atagaatttc
      241 ccgatgggca gattgaacag gatttcctgt gctttttcag gcgtgatcac gctcaggccg
      301 tccggtttct ccaattcgga gccgatcttg gccaggaatt tgttgaagga aaccccggcc
      361 gaacaagtga gttgggtggc ggccagaatg tcggctttga tgcgccttgc gatctccacg
      421 ggatcatcca gccccgcttt gtttgtggta acgtccagat atgcctcatc cagggacatc
      481 gtttccacaa gatcggtata gctgaaaaag acggcccgaa tctgctccga gacctcttta
      541 tacagcttga aacggcagcg caaaaagatt ccctgagggc agagtttcca ggcctgatgc
      601 gaactcatgc cgctgtgaac gccaaatttg cgcgcttcgt atgaacaggt ggaaaccaca
      661 ccccggccat tggggttgcc gccaacgatc acacattttc ccctcagcga ggggtcctcg
      721 cgaatctcaa tcgccgcgaa ataagcgtcc atgtcgatgt ggatgatctt gttcacgcgg
      781 ccagtttgct atcagcaccg gattggtcaa ggctttattt caggcttatc tactgccatg
      841 tcctgctggt cacaccctta gcgctcgttt caatcgcact ccaacgcctt ccgcccaatc
      901 ctcgcatcaa atgcgggtat catgcgggag gcgcgactgt gacacatgaa agggccttaa
      961 cggccagaga gggtcgaaag gcttaaaaaa gaaatgccgc gcctcgaaag ggcggcatta
     1021 tcaggttatt taatataggt gttacagttg cagcataatc tccaccctgc ggctcttgcc
     1081 gagggcggaa ggttcggttt ggggatccag ggtgcgggtc ttgccatagg ctttccacga
     1141 tacgcgtttt ttggcgatgc ctttgctgat gagatagtct gccacggcac gggcgcgacg
     1201 ctcagagagg tccttgttct cggccgcgga accagattcc caggcatgcc cggaaagttt
     1261 gaccttcagg ccctggttgg tgagcaggct catcaccagc aggtccaatt ccgcggtggc
     1321 cccgcttcca agctcaaaac tgccgttttc gaaaaggatg tcaggcaggc tgaggctggc
     1381 ttttttctcc agtttctgca gctcgatgtc cagcttgacc ctggccaatg acctcgggtc
     1441 ctggatgatt tgctggtgaa caaggtatcc gggggtgttt ataaccgcgg tataggtatc
     1501 cgccaagggc agggtgccga ggaaattgcc cagggaattg gtgttgaaca cttcaactgt
     1561 cctctgccca ttcgtttcgc cgctgatctg gacctcggtc agcacagcct cgccaaactg
     1621 gtcatgcaca gagccttcaa cccggataat gatgggttca acgtcttccg ccaaggcttc
     1681 tgtgggttga ggttcgcgtc ccaggggagc aggcacgaaa cccggtttgg gcaggccctg
     1741 cctgatggtt tccaagctct cggcgatccg agcaaagatg gtttcctcgt cagctggctt
     1801 cctcagggcg ggggccacat attcgacatc cacgctgcgg acagagccgt cggcttcacg
     1861 aacgagatag ctggtgggcc tggcgagcga gaaattgatc tggtagatct tttcaaaacc
     1921 gcttacctgg cggtcagagc ttatatagcc ttcgttgctg ccgcggacat ggtagaaata
     1981 gcggtcgttg cgggtggaat tcaccggcag gcccagattt tctggcaaag accattcctg
     2041 ccaggtggtg ccgatccggt aagccttgaa gaggtcataa ccgccgaagc cgggatggcc
     2101 gcgggacgag aaatacagtg ttcttccgtc ccaatccagg aaaggcgttt gttcgttttc
     2161 cggtgtgttg atcatcggcc ccaggttttg aggttgggtc cagatgccgc cgatcttttc
     2221 acttacatag atatccgtgc cgccgtatcc gccttcccgg gaggaggcga aaaagagcag
     2281 ccggtcgcgg aaaaccatgg gatgggtttc cacctgcgag gagttaagtt gggagatgtt
     2341 ttggggctgg aaccatttat cggtgcgggg cacgaaatag atgtcgccgt cgcgtttgtt
     2401 ggtttcatag ttgccaaata tccaagcgcc ctgtggatcg gcggagaagc agccgaaagc
     2461 ttcgtttttg tcggtggaaa gttcatccac cagctccggc tggccaaatc cggagctgaa
     2521 aatctccgaa cgccagatgt tttccttgcc ttttttagcc ggacggaggg aagtgaaata
     2581 gaggtacttt ccctcggggt ccaggatggc gccgtaatct gaattgcggc tgttcacata
     2641 atccggcaga ggacggatgt tcaccttgct gaatatcttt ttctggcgct gcactatccg
     2701 tagataattg tcaacctggg attgatactg cttatctccg gaatcaaggt atttgtcgaa
     2761 ccaccgcatc gcgctggcgg tatccgcaac ggcaatgtgc agtcttccca gccagtaatg
     2821 gtattccggg accctgccgt cagcggagcg gtccgcgctt aggaattcac ggatggcccg
     2881 gttgtattcg ccacgctgat aatattccag gccctcctgc gcgtagcgcg agcccggaga
     2941 cctgatctgg ccgaaagcca gggctgccat gccgaccagc agcagaatga tcgctgtttt
     3001 tttcatcacc gctcccattt ggtcagaacc tcaggatgag ggacagaatg tggtcattgc
     3061 cgaaaacctc gtggttattg tagcggaaca catagtccag ctggaagttg cgcagcttta
     3121 tcccagtccc ggcggaaagc cggccgcggt tgtagcccag ccgtaatccg gtttgcaggg
     3181 tggaaagcgt ttcctcccat gaactttgct cgctcacgga gacgacagac agcttggaat
     3241 ccgcctccgg atctctgccg atcgaggtcc aatactcggc tccgaaacag aagtaggggt
     3301 cttcgtcctt ttccattttg gcatcggctg ccagggttaa gcccttcagg ggataagccg
     3361 ccaggccggc cgaaatctgg taaggcactg tggcgccatc gaattcgcca tacaggtcgc
     3421 gggccattgc cccgatttcc agatactggt tgatgtcata cttcgcgccg aagtccactc
     3481 cgtatccgta gatcgattcg ccctccaggg tgctcagata gaacttgggg ccaaagccaa
     3541 agctgaaatc accaatgctg ttggcatagc tgagggtgaa gttgttttcg ctgtctgaga
     3601 aggtgccggt gggattttcg ttttcatcgt agccgtcaat gtccagaacg ttggcgttca
     3661 cccaggacag cgccagagcg ccgaatctga agcggtttcc gaaggaagcg tagttgtggc
     3721 tgcggttgta gcccatgtcc aaattgacca tggtgcccag ctcgaaatcc ttcatccgcg
     3781 ttagcccggc cgggttccag tagccagaca cgatatcgtt ggttacggtc gatccggctt
     3841 cgcccatggc tataacgcgg gctccgactc ccatgcggga ataggccaaa agtgaattgt
     3901 tcatctcctg ggccgcgatg ggtgtggccg caagcacgag cagcacaacg ggcaggatgt
     3961 agttcattat ctttatcgtt ttcatctttg cctccttatt tgaccgcgat cttgatcacg
     4021 tgttcagacc tgttcacgcc gtcattagct acaacgcggg caaaataggt tccccggccg
     4081 acttttgcgc cggagctgtt cttgagattc cagggaatcc tgatctgatt tgagttctgg
     4141 gacggttcag tgatagtttg gaccttcttt ccggcaaaat cgtagatcga gacggtgagg
     4201 gtcacattgt tgttgcggtt gaggataacc acgatttcgg tctgctcggt tgctggattt
     4261 ggccaggcgt atgaggcgtc gctgatatcc aggtcctgag actgatagta ttccaccgtg
     4321 gaagcgctgg ccgggctttc agtcccgcca tacacagcgg tcacgtaata ttcgtaggaa
     4381 acttccattt cgatgctgta gtcatcgtaa taatagtttc catccacttg agtattcacc
     4441 agagaagtat tgattttagt gaagtctgat tccgtcagtt tgcggcggta aacgttgaaa
     4501 ccatccaggc ctttgccagc atcgggtagg ttccacagca ccctgatgta accagcgtag
     4561 gtgaatccgc gcacatcgat cggcgccgag aagatggagt aagtggcgct ggccacaggg
     4621 ctaggggtcc agtcggcttt gaatgcccgg gccgagatca ccatgttctg cgcgaagttg
     4681 ggcactatca caggttcggt gtagagggct gattcagccg tgggttcgct accgtcggtg
     4741 gtgtagtgga tctcggaatc ctcggtggga gatgagatca ccacaaagac cggcgcaccg
     4801 aa
//
LOCUS       DAOOWR010000111         4729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18107_length_4729_cov_3.150407, whole genome shotgun sequence.
ACCESSION   DAOOWR010000111 DAOOWR010000000
VERSION     DAOOWR010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18107_length_4729_cov_3.150407"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3732
                     /locus_tag="PLB85_03790"
     CDS             <1..3732
                     /locus_tag="PLB85_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72482.1"
                     /translation="NYNLYAPYDVQTNGFGNFRLPTATDLIYWNNMAELFVAEDYDGA
                     QAILTSNSAPFQIVQFNDTDTGRTYIMLREIPNPAYYDDNGTVTEDDDESGAFVYGWG
                     LFIYSPQGSKPIIITVPHPCDDFPTPAMGYEALKHWDARYLMINGSGREVRWTNQGSY
                     TNSKSLSDPLRNPTHPFNTLYKKFADTIRDQFGVREFSAQIHSYDWNRHQGYANCQIS
                     AGNPRPCPNLPIRDLSPLKRDLINAGQHLMIPANTIGIHADVYLNDFYSVNYSTHDFI
                     FSDGVNSYPVNDQVDLPAYTQNQHMLYTQSGLTDYDVYEPFFHLEMDELPNCYDETEN
                     VYHWFYGWDAASQSWNMDGLFDRFIGYYGRWIYDLEQVLVDMFAMNDNQTPPAPQNLA
                     IYNQSLTSVTLSWEKSHAYDFESYEVLYATESIGTDNFQVFNRDNNSFLASPDCEMIT
                     VTNLTNSNPYYFRIRAKDKNGNYSTLSNEVGANLAPAGITSFTAHGLDGTVRLFWQVN
                     GQIGNQGFKVYRQDAGGDFALVDSWLTNPALSNPSSYNFEWWDTDVSNGSGYTYKISS
                     TNLQNVEYFHNYPADAEPRPIHSLTVSNMSGTLSDEIFFSANPFATDGNDAYWDVTKG
                     TPGASNYVWGAFFEQYWSNSGTNLSREVKAMFDADSEIKTWVLRVRSDQIETLNITAS
                     GNFSREEKLYLQNGGTYHNLLAGPYQYSNTNSDFRTMILHWGNMQPRASFASLENKVY
                     QGGTDITFGWSFQYPFLIDHAEISIVNASDSLLLSDSVTPTQTSFPWLVPANVAEMQD
                     CRFVVDIVAVDGVRGRFVSPYRFSLLPQMILAYNEAGLKMRSNPWPDSDLSFEQVFGP
                     ALGYQMTPNGGWGMTLDFDFGTAYWVSSNDINFYSSILPLQSGAYYIDLVPGWNFLPN
                     PHLCAYDLEDLSFTLIGQLHSFTEMLSQELVSPAVFVWRGEDYEKTSRIEPWEAFLIY
                     YNGSPDLAPQIRFLPFCDGPKPLAPEPVFRLRMELEGAHPAGIELGLHPLATNAVDFR
                     FDHPSPPCPPISDCSSLWIHHAGADSTDIRNLCSEYRQPFSGPEQVEYFNIHAHAGLE
                     GPHEISFSTEGAGDQWQIMLMLDDVPHYLNGQDTFTWEPPATGTYEGYIRVSNYQVAL
                     QDLVQSPISALSAYPNPFNPDVTIAFNLALSNEVSVDLFNIRGQKVRSLHKGKLAGGN
                     HRLRWDGRDETGRGVASGVYFARVSTASETKTIRMLLLK"
     gene            complement(3978..>4729)
                     /locus_tag="PLB85_03795"
     CDS             complement(3978..>4729)
                     /locus_tag="PLB85_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOU72483.1"
                     /translation="MQVYAWKEVEDFARVFYKPLAEQKRGDHAALHKHLQNAVERYKQ
                     LENDDDRDKFRDKLKAYVRLYSFITQLISYTDQEQEMLYSFGRFLLQHIHPSDSRNAY
                     PEKDVELQYYRLQKVKEGSINLSEGKEVKVKSPTVTGTRKAREEEKPLSEIIETMNER
                     FGTDFSEADRLFFEQIKETAMQDEGVLKTAAANPLDKFELGIQQIIKDLMMKRLKDND
                     KIVSRYMEDEKFQKVIFSILAKDIYGTISTK"
BASE COUNT         1075 a         1417 c         1120 g         1117 t
ORIGIN      
        1 aattacaacc tctacgcgcc ttacgacgtc caaaccaacg gatttgggaa tttccgcctt
       61 cccacagcca ctgacctgat ctattggaac aacatggcgg aactcttcgt ggcagaggat
      121 tacgatggcg cacaggccat actcacatcc aattccgccc cttttcagat tgtgcagttc
      181 aatgacacag acaccggacg cacctatatc atgctgcgcg agattcccaa tcccgcctat
      241 tatgacgaca acggaacggt tacggaagat gatgacgaat cgggcgcatt tgtctatggc
      301 tgggggcttt tcatctacag tccccagggc agcaaaccca taatcatcac cgttccccat
      361 ccttgtgatg attttcccac tcccgcgatg ggatatgaag ctctgaagca ctgggacgcc
      421 cgctacctga tgataaacgg ctccggccgt gaggttcgct ggaccaacca gggttcttac
      481 accaactcca aatccctctc cgaccccctg cgcaacccca cccatccttt caacaccctc
      541 tacaagaaat ttgccgacac catccgcgac cagtttggcg tgagggagtt ttctgcccag
      601 atccattcct acgactggaa ccgccatcag ggctatgcca actgccagat ttctgccggt
      661 aatccgcgcc cctgccccaa cctgcccatc cgggaccttt cgcctctgaa gcgcgacctg
      721 atcaatgccg gccagcatct gatgattccc gcgaacacca ttggcataca tgccgatgtc
      781 tatctgaacg acttttacag cgtgaactac agcacgcacg atttcatttt ctcggacggc
      841 gtgaacagct atcccgtgaa cgaccaggtg gacctgcccg cctatactca gaaccagcac
      901 atgctttaca cccagagcgg tttaaccgat tacgacgttt acgaaccttt cttccatctg
      961 gaaatggacg aactgcccaa ctgctatgac gaaacggaaa acgtgtatca ttggttctac
     1021 ggctgggatg cggccagcca gagctggaat atggatggcc ttttcgaccg cttcatcgga
     1081 tattacggac gctggatcta cgatctggag caggtcctgg tagatatgtt cgccatgaac
     1141 gacaaccaaa ccccgcctgc gccgcaaaac ctcgccatat acaaccagtc cctgaccagc
     1201 gtcactctga gctgggaaaa aagtcatgct tacgattttg aatcctacga agtgctctac
     1261 gccacggagt cgatcggcac cgacaacttc caggtcttca accgggataa caactctttt
     1321 ctcgcctcac ccgattgcga gatgatcacg gtgaccaacc tgacaaactc caatccctac
     1381 tatttcagga ttcgcgccaa agacaagaat ggcaactatt ccaccctttc caacgaagtt
     1441 ggcgccaacc ttgcccctgc tggcattacc tctttcaccg cccacggcct ggatggcaca
     1501 gtgcgccttt tctggcaggt gaatggccag atcggcaacc aaggtttcaa ggtctatcgt
     1561 caggacgccg gcggcgactt cgctctggtg gattcctggc tcaccaaccc cgccctttca
     1621 aatccctcca gttacaactt tgaatggtgg gatacggatg tgagcaacgg cagcggctat
     1681 acctacaaaa tcagtagcac aaacctccag aatgtggagt atttccacaa ctatcccgcc
     1741 gacgcggaac caaggcccat ccacagcctc accgtcagca acatgtcagg gacgctatcc
     1801 gacgaaatat tcttcagcgc caatcccttc gccaccgatg gaaacgacgc ttactgggac
     1861 gtgaccaaag gcacccccgg cgcctcaaat tacgtctggg gcgctttctt cgaacagtat
     1921 tggagcaaca gcggcaccaa cctaagccgg gaagttaagg caatgttcga tgccgattct
     1981 gagatcaaga cctgggtgct cagggtccgc agcgaccaga tcgaaacgct caacataact
     2041 gcatccggca acttcagccg ggaggaaaag ctctatttgc agaatggcgg cacctaccac
     2101 aacctgctgg ccgggcccta ccaatacagc aacacaaatt ccgacttccg caccatgatc
     2161 cttcactggg gaaacatgca gccccgggct tcctttgcct cgctggaaaa caaagtatat
     2221 cagggcggaa ccgatatcac cttcggctgg agtttccaat atcccttcct catcgaccat
     2281 gccgaaatct ctattgtcaa cgcttccgac agtctgctgc tttcggactc cgtgacgccc
     2341 acccaaacca gctttccctg gctggtgccg gccaacgtgg ctgaaatgca ggattgccgc
     2401 ttcgtggtgg acatcgtggc ggtggatggg gtgcgcggca ggtttgtatc cccctatcgc
     2461 ttttccctgc ttccccagat gatcctggcc tataacgaag ccggcttgaa gatgcgctcc
     2521 aatccctggc cggatagcga cctcagcttt gaacaggtct ttggccccgc cctcggctat
     2581 cagatgaccc cgaatggcgg ctggggcatg acgctggatt tcgacttcgg cactgcctat
     2641 tgggtttcat ccaacgatat caatttctac agcagcatct tgccgctcca gagcggggct
     2701 tattatattg atttggtccc cggctggaac tttttgccca atccccatct ctgcgcctac
     2761 gacttggagg acctgagctt cactcttatt ggccagttgc acagcttcac cgaaatgctg
     2821 agccaggaac tggtttcgcc cgccgtcttt gtctggcgcg gcgaggatta cgaaaaaacc
     2881 agccgtatcg agccctggga ggctttcctc atctattaca acggctcccc ggacctggcg
     2941 ccgcagattc gtttcctgcc tttctgcgac ggtcccaagc cgctggctcc ggaacccgtt
     3001 tttaggctgc ggatggaact cgagggagcc catcccgccg gaattgaact cggcctgcat
     3061 cccctggcta caaatgccgt ggatttccgc tttgaccatc ccagcccgcc ctgtcctccg
     3121 atttctgatt gctcctctct ctggatccat catgccggcg cggattccac tgacatccgg
     3181 aacctttgca gcgaataccg ccagcccttc agcgggccgg aacaggttga atacttcaac
     3241 atccatgccc acgcgggcct ggaagggcca cacgagatca gtttcagcac cgagggagcc
     3301 ggcgaccaat ggcagattat gctgatgctg gatgacgttc cccactatct gaacggccag
     3361 gatactttta cctgggagcc tccggctaca ggaacctacg aaggctacat ccgggtgagc
     3421 aattaccagg ttgctctcca ggatctggtc caaagcccca tatccgcgct gtcggcctat
     3481 cccaatccct tcaatccaga tgtcaccatc gccttcaacc ttgccctctc aaacgaggtg
     3541 agcgtcgatc tcttcaacat tcgcggccag aaagtgcgct cccttcacaa aggaaaactc
     3601 gcgggcggaa accacaggct gcgctgggac ggacgtgatg aaaccggaag gggagtggcc
     3661 agcggtgtgt atttcgcccg ggtgagcaca gccagcgaaa ccaaaaccat caggatgctg
     3721 ctgctgaagt aagcgatttt caggaactgc ccggcgccgt ctgtgcgccg gatggatttc
     3781 agcctgggtg atacaggcca taaagcctga taaaaaagca gccccccagc gtaaatggaa
     3841 agttttgcct gattgcaaca ataacattgg gttgagatca tgcatgatac cctccacttg
     3901 aaaccactct gagtagctat cttatagcgg gatgtaagtc acaaacatcc cgtaacatat
     3961 actcgtttat gtttatctta cttggtgctt attgttccgt aaatatcttt tgcaagtatg
     4021 gagaatatca ccttctgaaa cttctcgtct tccatgtacc ggcttacgat cttgtcatta
     4081 tcttttagcc gtttcatcat caggtctttg atgatctgct gaatacccag ctcaaactta
     4141 tccaggggat tggcagcggc tgtcttgagc acaccttcgt cctgcatggc agtttccttg
     4201 atttgttcaa agaagagccg gtctgcttcg ctgaagtcag tcccaaaacg ctcgttcata
     4261 gtttcgatga tctcagatag aggcttttcc tcttccctgg ctttacgggt gcctgtaacg
     4321 gtgggagatt tgaccttgac ctctttgcct tcagatagat tgatggagcc ttccttcacc
     4381 ttctgcaggc ggtagtattg caattcgaca tctttttctg gataagcatt ccggctgtca
     4441 ctgggatgga tgtgttgaag caggaagcgg ccgaagctat agagcatttc ctgctcctga
     4501 tcggtgtagc tgatcagttg ggttatgaag gaatagagtc gcacatatgc cttcagcttg
     4561 tcccggaact tgtccctgtc atcatcgttc tcaagctgtt tatatcgttc cacagcattc
     4621 tgcaggtgtt tatgcaaggc cgcgtgatct ccccgtttct gttcggccag aggtttgtaa
     4681 aagaccctgg caaagtcctc cacttctttc caggcataga cctgcattt
//
LOCUS       DAOOWR010000112         4693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18286_length_4693_cov_4.202889, whole genome shotgun sequence.
ACCESSION   DAOOWR010000112 DAOOWR010000000
VERSION     DAOOWR010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18286_length_4693_cov_4.202889"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLB85_03800"
     CDS             <1..492
                     /locus_tag="PLB85_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72484.1"
                     /translation="LLQDEALGLISENLNTSLLSPLLYPLENNIRRLLRIDDFSIRAG
                     FIENLFTKYTTNPNQLTGYEDLNQFVGNVSQFSSSILLNNLSIYMSKYLGRKFFLDYT
                     LGLQEATDLQNRTQIVVSHEASLRWYLPAKFRLAYTFKYVPWDEQISHELMLQRSFKF
                     WGL"
     gene            527..1090
                     /locus_tag="PLB85_03805"
     CDS             527..1090
                     /locus_tag="PLB85_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72485.1"
                     /translation="MQKTIIIREETMPDNEIKDKQAEATKPAPKPERKPQPPQAENEK
                     PRSKLGLVEILMFLLLAGVVFIFIFGMQQQKRDKELELAMQQKVEELKPIFMDIAKSA
                     KEYKANDPFGDWPLTVDELNIDTTNLKTEEYAFEWLDSGTVVLTTTEKFGKEGVKISY
                     DVEGDSYSIEDPDAGSRPQIKESWFNQ"
     gene            complement(1406..2098)
                     /locus_tag="PLB85_03810"
     CDS             complement(1406..2098)
                     /locus_tag="PLB85_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOU72486.1"
                     /translation="MPAKNKPRKSKGSSWLDRIPLPTKQSLGEEIANSITHGTGVGLS
                     IAALVILVVFAARKGDTWKIASFSVYGATLIILYLASTLYHAITHPGLKRFFRVLDHC
                     SIFLLIAGTYTPVTIGVMRGAWGWTLFGVIWALAIVGINLKIFALDRRKKLSLLIYLL
                     MGWMIVIAVKPLLSYLEPASLIWLFVGGACYTLGIIFFVLKKLPFAHSVWHLFVLGGS
                     ICHFFAMLNLVA"
     gene            complement(2088..2672)
                     /gene="nadD"
                     /locus_tag="PLB85_03815"
     CDS             complement(2088..2672)
                     /gene="nadD"
                     /locus_tag="PLB85_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU72487.1"
                     /translation="MICQALLGGSFDPVHNGHLHIAREILRSGIAQTVVFLPNARHNF
                     KRGSVLLDYPRRRELVAAVLEPGMEVWDDDAQGSGYTADLLKHLYQKHPDHVFLWVIG
                     SDNIAGLPLWYNFQWLKANARFLIIPRPGYPADEKILKRIRRKTLKISPSEVSSTLVR
                     DRIKAGESITDLVPERIAKRVLRLYKPLLAGHAR"
     gene            complement(2669..3388)
                     /gene="bamD"
                     /locus_tag="PLB85_03820"
     CDS             complement(2669..3388)
                     /gene="bamD"
                     /locus_tag="PLB85_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOU72488.1"
                     /translation="MKYPLILIILILLLSACAQNRSTLTTEEKLAKADELYELGKYTR
                     AADLYGDVYFERQSASSARALLRQADCLFKANKFAEARLLYQEFSDSFPTHAEVSTAV
                     FRYAVCLFEESLGPQYDQTETIQSIDAFRRFLEKYPKSERYADAIDYIQKAQYKLIEK
                     RFRTGYIYYKMRDYSSALMYFKEVAELGNTDRLDRESLYYSALLLYKQKLKDQAAVEY
                     DRLKAKYPDSKEAKKLAKYFK"
     gene            complement(3398..3976)
                     /locus_tag="PLB85_03825"
     CDS             complement(3398..3976)
                     /locus_tag="PLB85_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72489.1"
                     /translation="MKKLLVLLLALMSLGLAAEAQSASAEPEIISEQIVELIDVSRPL
                     ILNIQAGDLTPRLDFLIRGLLLSKGADLREADPLSGLAFSAAEGDSLADLAPYALQSL
                     NLVSIGLELGGTTVEHKNFLSYRSERFPLYTFQVKQISLPGYKLLRIDELSFTDRELV
                     SSGTSISSLKWFEPVLATTALASLIFLLWTIE"
     gene            complement(3985..>4693)
                     /locus_tag="PLB85_03830"
     CDS             complement(3985..>4693)
                     /locus_tag="PLB85_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72490.1"
                     /translation="NLEKEALAELQASQTTLTEDQQKSAAQEKEAIWTEITRIRGELT
                     KSQNELTAIRQTLSSDVEGLKKDRTQVMTEMSELRERVYAVNSDLTSLTTDLQSVITK
                     ERAAAEKRRLEAINKQYKAALAEYNRGRHENSIVLFEEFLKANPDCELSPNAYYWIGE
                     NYYSAGNYPKALRQFQEVVDRYQGHDKAWDAQLKIGLTYFQMQDYESTYNELMVIKNY
                     NPSYPQMKIVDKYLKRI"
BASE COUNT         1086 a         1197 c         1344 g         1066 t
ORIGIN      
        1 ctgctgcagg acgaggctct ggggctgatt tccgaaaacc tgaacacctc gctgctctca
       61 cctctgctct accctctgga aaacaacatc cgccgcctgc tgaggataga cgacttcagc
      121 atccgcgccg gattcatcga aaacctcttc accaaataca ccaccaatcc caaccaactg
      181 accggatatg aggacctgaa ccagtttgtg ggtaatgtgt cccaattcag ttcttccatc
      241 ctgctcaaca acctttccat ctacatgagc aaatacctgg gccgcaaatt cttcctcgac
      301 tacaccctgg gcctgcagga agccaccgac ctccaaaacc gtacccagat cgtggtctcc
      361 cacgaagcct ctttgcgctg gtatcttccg gcgaagttcc gccttgccta caccttcaaa
      421 tatgtgccat gggacgaaca gatttcccac gaactcatgc tccagaggtc attcaaattt
      481 tggggattgt aacaaaagct ttgacaaaat atgccgtctg gaaataatgc aaaaaacgat
      541 aatcattagg gaggaaacca tgcccgataa cgagatcaaa gataaacagg ccgaagccac
      601 caaacccgct ccaaaaccag agcgaaagcc ccagcctcca caggcggaaa acgagaaacc
      661 acgcagcaaa ctgggcctcg tggaaatact gatgttcctg ctgctggcag gggtggtctt
      721 catcttcatc tttggaatgc agcagcaaaa acgcgacaaa gaactggaac tggccatgca
      781 gcagaaagtt gaggaactga agccgatctt catggatatc gccaaaagcg caaaagagta
      841 caaagccaat gatccctttg gcgattggcc cctcaccgtc gacgaactta acattgacac
      901 gaccaattta aaaaccgaag aatacgcttt cgagtggttg gatagcggca cagtcgtgct
      961 gaccaccacc gagaaattcg gcaaagaggg cgttaagatc agctacgacg tggaaggcga
     1021 ttcctactcc atcgaagacc cagatgccgg ttcccggccg caaatcaagg aaagctggtt
     1081 caaccagtaa aacccgaatc caatcccatg atttaaaacc cgcccacaac ggcgggttat
     1141 tttgtgtctt taaacggacg cgcggatatt tttacagctc tgggggaagt ttgttaccca
     1201 ctatgttgaa aagggttatg tcgcaagcaa ccggaaacat cctgccaaaa cgcggatagg
     1261 ccctttgccc cttccaactc acatcccata cacttcccgt ggacttcccg cctgccaaac
     1321 gggaactcag cgggaggcga taaggaggcg gattggaagg ggctaaggac gggtcaagcc
     1381 caagtttggc agcaggcatg ttctttcacg ccaccaggtt aagcatcgcg aagaagtggc
     1441 agatgctgcc tcccagcacg aagaggtgcc agacgctgtg ggcgaagggc agctttttca
     1501 gcacaaagaa aattataccg agggtgtagc aggccccgcc gacgaacagc cagatgaggg
     1561 aggcgggttc cagatagctc agaagcggct tcacggcaat aacgatcatc caccccatca
     1621 gcagataaat gagcagggag agctttttcc tgcggtcgag agcgaagatt ttcaggttga
     1681 tgccaacgat cgccagagcc cagataacgc caaacagcgt ccagccccag gctccgcgca
     1741 tcacaccgat ggtcacggga gtgtaagtgc ccgctatcaa aagaaatatg gagcagtggt
     1801 cgaggacacg gaaaaagcgc ttgaggccgg gatgggtgat ggcgtgatag agggtggatg
     1861 cgaggtaaag gatgatcaga gtggcgccgt aaacgctgaa ggaggcgatc ttccaggtat
     1921 ctcccttccg ggcggcaaac acgaccagaa tcaccagagc ggcgatgctg agcccgactc
     1981 cggtgccgtg ggtgatgctg ttggcgattt cctcgcccaa actttgtttg gtgggcaggg
     2041 gtatccggtc aagccagctg gagccctttg atttgcgggg cttgttttta gcgggcatgg
     2101 ccggccagaa gcggtttgta cagcctcagc acgcgtttcg cgatccgctc cggaaccagg
     2161 tccgtgatgg actctccggc cttgatccgg tcgcggacaa gggtggagga cacctcgctg
     2221 gggctgatct tcaaggtttt gcggcggatg cgcttgagga ttttttcgtc ggcgggatag
     2281 cccgggcggg gaatgattag aaaccgcgcg ttggctttga gccattggaa gttataccag
     2341 aggggtagac cggcgatgtt gtcggaaccg atcacccaga ggaaaacgtg gtccggatgc
     2401 ttctggtaaa ggtgtttgag caggtcggca gtatagccgc tgccttgggc gtcgtcatcc
     2461 caaacttcca tgcccggttc cagcacggcg gccaccagtt cgcggcggcg cgggtagtcc
     2521 agcaacacgc ttccccgctt aaagttgtgt ctggcattgg gcaggaagac aacagtctgg
     2581 gcgatgccgc tccgcaagat ttccctggcg atgtgcagat gtccgttgtg aacggggtcg
     2641 aagctgcctc cgagcaaggc ctggcaaatc atttgaagta cttggccagt tttttcgctt
     2701 ccttcgaatc ggggtatttt gctttcagac ggtcatattc gaccgcggct tggtctttca
     2761 gtttctgttt atagagcaaa agggcagaat aataaaggga ttcgcggtcg aggcggtctg
     2821 tgttgccaag ttcggccact tccttgaaat acatcagggc ggagctgtaa tccctcatct
     2881 tatagtagat gtagccggtc ctgaacctct tctctatcag cttgtactgc gctttttgga
     2941 tgtaatcgat ggcatcagca tagcgttcgc tttttggata cttttccagg aagcggcgga
     3001 aggcatcgat ggactggatg gtctcggtct ggtcatactg agggccgagg gattcctcaa
     3061 aaaggcagac cgcgtaacgg aaaacagcag tggatacctc ggcatgggtg gggaaggaat
     3121 ccgaaaattc ctgatagagg agccgggctt cggcgaattt gttggccttg aagaggcaat
     3181 cggcctggcg cagcagggcg cgggcagagg aagcggactg gcgctcgaag taaacatcgc
     3241 catagaggtc cgcggcgcgg gtgtattttc ccagttcgta aagttcgtcg gccttggcca
     3301 gtttttcctc ggtggtgagg gtgctccggt tctgcgcgca ggcagagagc aacaggatca
     3361 ggataatcag gatcagggga tatttcatgc ggattcctta ttcgatcgtc caaagcagga
     3421 agatcagcga ggccagggcg gtggtggcca atacgggttc aaaccatttg aggctggata
     3481 tagaggttcc gctggatacg agttcacggt cggtgaagct cagttcgtcg atacggagca
     3541 gtttgtagcc cggcaggctg atttgcttca cctggaaggt gtagagcgga aagcgttcac
     3601 ttcggtagga aagaaagttt ttatgctcga cagtggtgcc gccgagttcc aggccaatgc
     3661 tgaccagatt gaggctttgc aacgcgtagg gagcaaggtc agccaggctg tccccctccg
     3721 ctgctgaaaa tgccaaaccc gacaaagggt cggcttcacg caggtcggct cctttggaga
     3781 gcaacagccc acggatgaga aaatccagcc ggggagtgag gtccccggct tgaatattca
     3841 ggattagcgg gcgtgaaacg tcgatcaatt caacgatctg ctcgctgatt atttccggct
     3901 ccgcgctggc ggattgagcc tcagccgcaa ggccaaggct catcagcgcc aatagcagga
     3961 ccagaagctt tttcattagg atgttcagat tctcttcaga tacttatcca ctatcttcat
     4021 ctgaggatag ctgggattgt agtttttgat caccatcagt tcgttgtaag tggattcata
     4081 gtcctgcatc tggaaatagg taaggccgat cttcaactgg gcgtcccaag ccttgtcgtg
     4141 gccctgatag cggtccacca cttcctggaa ctggcgtagc gctttggggt agttgccggc
     4201 gctgtaataa ttctcgccga tccagtagta ggcgttgggg ctgagttcac aatcgggatt
     4261 ggctttcagg aattcctcaa agagcacgat ggagttttcg tgccggcctc tgttgtattc
     4321 tgcaagggct gccttgtact gcttgttaat ggcttccagg cggcgttttt ctgccgcggc
     4381 ccgttctttc gtgatcacag actgcagatc ggtggtgagg ctggtgaggt cgctgttgac
     4441 ggcatagacc cgttcgcgta attcgctcat ctcggtcatc acttgcgttc tgtctttttt
     4501 gagtccttcc acgtcggaac tcagggtctg gcggatggcg gtgagctcgt tttgggattt
     4561 ggtgagttca ccgcggatac gggtgatttc ggtccaaatt gcttcttttt cctgggcggc
     4621 gctcttttgc tggtcttcgg taagggtggt ctgtgaggct tgtaactcag ccagggcctc
     4681 tttttcgagg ttg
//
LOCUS       DAOOWR010000113         4693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18287_length_4693_cov_3.563605, whole genome shotgun sequence.
ACCESSION   DAOOWR010000113 DAOOWR010000000
VERSION     DAOOWR010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18287_length_4693_cov_3.563605"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLB85_03835"
     CDS             <1..526
                     /locus_tag="PLB85_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU72491.1"
                     /translation="LYKSLKGYIKNDQIEEAIKITEKLKGTTLGFIFFSGLSVYKDVR
                     KTASKEDMSDQVQNALDEAVLQKVYKLDAGLFWFDTLAQVCTYLGLLGTIWGLLAAFN
                     ALSRPDVLDSQKNIMLSAGIKTAIGTTALGLIAAIPLTLIKGWLMGKAQKLINEIDEY
                     SVKLINFINISAKG"
     gene            531..1067
                     /locus_tag="PLB85_03840"
     CDS             531..1067
                     /locus_tag="PLB85_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU72492.1"
                     /translation="MAYRPSARRNQHPVSDPKIPDLVPLMNLFLTVIPFLLLMLVITQ
                     VALVAFNFSAAPPGQSATVDGTEGLNKDVPEVTVIITASQDSLLTTIPGFEIREPGRD
                     PGIVKLSLGQYDYVMLDAALKAVRERFPDLLDISVAPYDDVKYDVLIKTIDICRSNNF
                     ANVHYKAPQVRYYVMGGK"
     gene            1064..1615
                     /locus_tag="PLB85_03845"
     CDS             1064..1615
                     /locus_tag="PLB85_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU72493.1"
                     /translation="MSNIIKPKDTNRRVRRKFKEGTSLSIISLVDILTILLVFLLKNV
                     SMEVQKLTMPNNMTFPVTMEKRDLLENKGTTVVQIYPDRILLGEQGIYFGSLEEFASD
                     PDKRTEILTYLQSTATEIVKTPDADGNPTTTALLIQADKSIPCWYITEFVSLGTSSFY
                     EYIYFATLLETDWLEKSKSNTSG"
     gene            1626..3140
                     /locus_tag="PLB85_03850"
     CDS             1626..3140
                     /locus_tag="PLB85_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72494.1"
                     /translation="MAKTILNISLNYRGKHLDTIRQGLDFTKKWYIGTDKHLFWQILD
                     DSQKFPVRHKLLIKRGEDFYLQLPDGSNLTCSKDGNPLDANALQQNGILSGSLLKLRN
                     DLSGTAQLNPDYDIQFGYTEPRIIVLRPEEQAVVNNSLKPAPLSSSERTETGLTLLFL
                     ILGLAFVLIYDLVIKPKMDQEKTLTETLAELERVRRIEPQLGLTPPEATMPQTPPEAQ
                     VSDTRGQQQPGGTQPARGTGRTTGGRQSQGVPDRGQIFGTGRPGVGTPGPSRPQTAYA
                     VTGLQNFITARPGARGSATGTGTGSGASGSFNPQASSGFSGSFDPNATSGFNTSTSDV
                     GRVATTLPGGPGLTTRPNVPTQTFTGDASRLKTPSTGQWPTVPSAREETERVTQSVVK
                     APVITKLPTPEVKIPKPASSVPDADIIYNQIASRKGQIEQSYKRNVAIKKQTGSITVV
                     MDIAENGSVTARVTSNSATFTQSFLNEIKNIVESWRFNVTKPTKYQFKMNLTQA"
     gene            3491..>4693
                     /locus_tag="PLB85_03855"
     CDS             3491..>4693
                     /locus_tag="PLB85_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOU72495.1"
                     /translation="MYYYAVHLPLALTREFHYSSKVEITPGARVLVSFNRKDMIGICG
                     DQMQKQPSDRIRYKPVLEILDDEPVLGPRLLELAKFMAEYYACSLGSACFAMLPAWLV
                     PDIDAEVKWLAADFPEPFRALQPALGGGETMKVPELRKQLKGVPVLHLIEQASDLGLL
                     ELKRKLSSRDKPKTVNYVRLLDREPDLGLFPPKQKEALQLILAIPKEEFPLSEISETV
                     SYSVVRALQKKGLLSITPKKVEREFFSFDSSAGPKVVTLNAEQQLAVQEISRQAAKFR
                     VDLLYGITGSGKTEVYIPLIRSCLQEGKGVIFLIPEIALTPQMVERFQGEFGATLAIS
                     HSQLSDRQRLNQWHKIAAGECRIVIGARSAVFAPMPRLGLIIVDEEHEQSYKQDNNPR
                     YNGRDLAVM"
BASE COUNT         1182 a         1355 c         1189 g          967 t
ORIGIN      
        1 cctgtacaaa agcctgaaag gctacatcaa gaacgaccag atcgaagaag ccatcaagat
       61 cacggaaaaa ttgaagggca ccaccctcgg cttcatcttc ttcagcgggc tttccgtcta
      121 taaggacgtg cgcaagaccg cctccaaaga agacatgagc gaccaggtgc agaacgccct
      181 cgacgaagcc gtgctgcaaa aagtttacaa actcgacgcg ggccttttct ggttcgacac
      241 tcttgcccag gtttgcacct atctcggtct gctgggaacc atttggggat tgctggcagc
      301 tttcaacgcc ctcagccgtc cagacgtgct tgacagccag aagaacatca tgctctctgc
      361 cggcatcaaa accgccatcg gcaccaccgc cctcggtttg atcgccgcca ttccgctcac
      421 tctgatcaaa ggttggctga tgggcaaagc ccaaaaactc atcaacgaga tcgacgaata
      481 tagcgttaaa ctaatcaact tcatcaatat ctcagctaaa ggataaaatc atggcatacc
      541 gtccttcagc aagaaggaac cagcatccgg tctcagatcc gaaaattccg gatctggtgc
      601 ccctcatgaa cttgtttctc accgtcatcc catttctatt gctgatgctg gtgatcacgc
      661 aggtggcgct ggtggccttc aacttctctg ccgcccctcc cggccagtcc gcgacggttg
      721 acggcacgga aggcttgaat aaggatgttc cggaagtaac cgtaatcata acggcctcgc
      781 aggattcact gctcaccacc atacctggtt tcgagatcag ggaacccgga cgcgaccccg
      841 gtatcgtgaa attgtctctt ggacaatatg actatgtcat gctcgatgcc gccctcaagg
      901 ccgtccggga aaggtttccg gacctgctgg acattagtgt ggccccttat gatgatgtga
      961 agtatgacgt cctgatcaaa accatagata tctgccggag caacaacttt gccaatgttc
     1021 actacaaagc cccgcaggtt cgctactacg tgatgggggg aaaatgagca acatcattaa
     1081 gcccaaagac acgaaccgcc gggtgcggcg caagttcaaa gaaggaactt cgctgtccat
     1141 catttcactg gtggatatcc tcaccatcct gctggtgttc ctgctcaaga acgtatccat
     1201 ggaagtgcaa aaactgacca tgcctaacaa catgaccttc ccggtcacca tggaaaagcg
     1261 ggacttgctg gaaaacaaag gtaccaccgt ggttcagatc taccctgacc ggatactcct
     1321 tggcgaacag ggaatctatt ttggcagcct ggaagagttt gcttctgacc ccgataaacg
     1381 caccgaaatc ctcacttact tgcaaagtac tgccacggaa attgttaaaa ctcccgatgc
     1441 cgacggtaat ccgaccacca cagccttgct catacaggcg gataaaagca tcccctgctg
     1501 gtatataacg gaattcgtga gcttggggac cagttctttc tacgaataca tctattttgc
     1561 caccctcctg gaaactgatt ggcttgaaaa aagtaaatcg aacaccagtg ggtaagggag
     1621 gtatgatggc aaagacgata ctgaatataa gccttaatta ccggggaaaa catctggata
     1681 ccatccgcca gggattggat tttaccaaaa aatggtacat tggaacagat aaacacctgt
     1741 tctggcaaat cctggacgat tcgcagaagt tcccagtcag gcataagctt ctaatcaagc
     1801 gtggagaaga cttctatctg cagctgccgg atggctcgaa cctgacttgc agcaaggatg
     1861 gcaatccgct ggatgccaat gccctgcagc aaaatggcat cctatcaggc tcgctgctca
     1921 agctgcgcaa cgacctcagc ggaaccgcgc agttaaaccc tgattacgac atccagttcg
     1981 gttataccga gcctcggata attgttctgc gcccggaaga acaagctgtg gtgaacaaca
     2041 gcctgaagcc ggctccgctt tcctcttcgg aacgcacgga aaccggactc accctgctct
     2101 tcctgattct tggcctcgct ttcgtgctga tctacgacct cgtgatcaag cccaaaatgg
     2161 accaggaaaa aaccctcacg gaaactctgg cggaactgga aagagtgcgc aggatagagc
     2221 cgcagctcgg tttgacccct ccggaagcaa ccatgccgca gactcctccg gaagcgcaag
     2281 tcagtgatac ccgcggacaa cagcaacccg gcggaacgca acctgccaga ggtaccggcc
     2341 ggaccaccgg cggaaggcaa agccagggtg tgcccgatag aggccagatc tttggcaccg
     2401 gaaggcccgg agtaggaact cccggcccca gcaggcccca aactgcctat gcggtcaccg
     2461 gcttgcagaa ttttatcact gcccgcccgg gagccagagg ttccgcaacc ggcaccggca
     2521 caggatctgg agcatcggga tccttcaatc cccaggcttc ttcaggtttc tccggttcct
     2581 tcgatcctaa tgccacttca ggtttcaaca cctcaacctc tgatgttggc agggttgcca
     2641 caaccctacc cggcggaccc ggattgacca cccggcctaa tgtgcccacc cagactttca
     2701 ccggtgacgc ttccaggctg aaaaccccct ccaccggcca gtggccgacc gttccatcag
     2761 cccgtgaaga aactgaaaga gttacccaat ccgttgtgaa agccccggtg ataactaagc
     2821 tccccactcc ggaagtgaaa atacccaagc ccgcttcctc tgttccggat gccgacatca
     2881 tctacaacca gatcgcctcc cgaaaaggcc agatcgagca atcctacaag agaaacgttg
     2941 ccatcaaaaa gcagaccggt tcaatcactg ttgtaatgga cattgcggaa aatggcagtg
     3001 ttacggcccg tgtaacttca aacagcgcca cattcacgca gagcttcttg aacgagatca
     3061 agaacatagt ggaatcctgg cgcttcaacg tgaccaaacc cacaaaatac cagttcaaaa
     3121 tgaacctcac tcaagcctaa gcataaacga gaacatatca aaaccccgct ccaccggcgg
     3181 ggtttttttc tgccttggat tatggatgtt tctggtttgc cgttggaccc tgtctcatct
     3241 ctagccattg atgaatgaag ccagggactg aaaatacaca ggaattcaga gattcgctct
     3301 ggcttcgccg tttgccccat cccatataca tcccgttcac ttcccgttga cttcccgttt
     3361 gggcagcggg aactcaacgg gatgcgaaaa ggaggcaagt gagctggggc aaagggcgaa
     3421 tttgccagga acagaagcca cgctggcaga tttccattga cacaaatgcc cctgccctta
     3481 gcctttgact atgtattact acgctgtgca tctgcccctg gccctcaccc gggaatttca
     3541 ttacagctcc aaagtggaga tcactcccgg agccagggtc ttggtgagtt tcaaccgcaa
     3601 ggacatgatc ggcatctgtg gtgaccagat gcagaaacag ccttctgaca ggatacgcta
     3661 caagccggtg ttggagattc tggacgacga accggtgctg gggccgcggc tgctggagct
     3721 ggcgaagttc atggcggaat actatgcctg ttccctgggc agcgcttgtt tcgcgatgtt
     3781 gccggcctgg ctggtgccgg atatcgatgc tgaggtgaaa tggctggcgg ccgattttcc
     3841 cgagcctttc cgggcgctgc aacccgcttt gggtggaggc gaaaccatga aggtcccgga
     3901 actgcgcaag cagttgaaag gcgtgcccgt gctgcatctg atcgaacagg cgtcggatct
     3961 gggtctgctg gagctcaaac gcaagctgag ctccagggac aaacccaaaa cggtcaatta
     4021 tgtcaggctg ctggaccgtg agcccgactt ggggttgttc ccacccaagc aaaaggaagc
     4081 cctgcaactg attctggcca tccccaaaga ggagtttccc ctctccgaaa tcagcgaaac
     4141 cgtatcctat tcggtggtgc gagcgctgca gaagaaaggc ttgctgagca tcacccccaa
     4201 aaaggtggaa cgtgagttct tcagcttcga cagcagtgcc gggcccaagg tcgtaacact
     4261 aaatgcagag cagcagctcg cggtgcagga gatatcgagg caagctgcga aattcagggt
     4321 tgatctgctt tacggcatta ccggcagcgg caagacagag gtttacatcc ctttgatccg
     4381 ttcctgcctg caagagggca agggagttat cttcctgatc ccggaaatcg cgctcacacc
     4441 gcagatggtg gagcgcttcc agggcgaatt cggtgccacg ctcgccatct cccacagcca
     4501 gttgagcgac cgccagcgcc tgaaccagtg gcacaagatc gccgcaggtg aatgccggat
     4561 cgtgatcggc gcccgcagcg cggtttttgc ccccatgccg aggttggggc tcatcatcgt
     4621 ggacgaggaa cacgagcaat cttacaaaca ggacaacaac ccccgctaca acggacgcga
     4681 cctcgccgtg atg
//
LOCUS       DAOOWR010000114         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18521_length_4648_cov_3.692793, whole genome shotgun sequence.
ACCESSION   DAOOWR010000114 DAOOWR010000000
VERSION     DAOOWR010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18521_length_4648_cov_3.692793"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLB85_03860"
     CDS             <1..740
                     /locus_tag="PLB85_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOU72496.1"
                     /translation="GIDFHLQIVCVPQYNCGDRLRATLSDLLAGSVNVLSIGVVPVGL
                     TGFRENLTELKPFTAELAAETIAIIDSFRQQGQPVQAADELYVMAGLSVPDESYYGDF
                     PQLENGIGMLRLSRMNFRRRRSALIKELEKAGMPFVMLTSRSAESTIKELAGRLEPKL
                     EKASVRVKAIANNWLGGQVSVSGLLAAKDILEQHNATGNEGLIVPSNIFNHDGITLDD
                     VSQIEFRDLLGRPLLVVDQYFEDWEWI"
     gene            790..1719
                     /locus_tag="PLB85_03865"
     CDS             790..1719
                     /locus_tag="PLB85_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOU72497.1"
                     /translation="MKQAALIVLALILLLASCMSEADKLEKANLELGSKKNPIKMYFV
                     PSMEASTVVTSGEAIAAWLEQETGYRFKVAVPTSYAAVVEAIGAYQADVAWLPTFAYV
                     LAEEKYEAEVQFMTVRNGLNKYCGQFVARADSGIDSLSQIEGKVVAYTDAASTSGYIY
                     PSAILKQQGINPARYTLAGGHPQAITAVYNGTADVACTYWSPPDASGKPMDAREKLLE
                     TYPDVFSKLKIVALTDSIPNDTVTFRRGMPDEVKTKLVQALEKFAGNKDGQAIMKELY
                     DVDGLTRATDADYDIVRQTLKALDKDPRQFLKD"
     gene            1726..2466
                     /gene="phnC"
                     /locus_tag="PLB85_03870"
     CDS             1726..2466
                     /gene="phnC"
                     /locus_tag="PLB85_03870"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOU72498.1"
                     /translation="MAQLEIKDLRKTYDHKVWALDKVSFDVQKGDFIILLGLSGSGKS
                     TLLRCINRLIEPDGGDVLYEGKSVRALSPTQLRRYRRSIAMVFQQFNLVKNLSVLTNV
                     LTGRLGYHGLLDKYSASEIDAAYHNLDRVGLKKYAKRQVKSLSGGQQQRVAIARALMQ
                     NPAIVLADEPVASLDPATADSIMQYLAEINSEGISVLCSLHFLSLARRYGNRVVALKD
                     GLKVYEGLPVEIDNARFKQIYGQDAEEV"
     gene            2835..3104
                     /locus_tag="PLB85_03875"
     CDS             2835..3104
                     /locus_tag="PLB85_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU72499.1"
                     /translation="MLTWELRATGRVQFVGFRRFARTCALRCSLTGWVRNQGDGSVLI
                     EATGDRDKLELFCELLRAGNRFVDVRELRIREQDETVVYDDFTIR"
     gene            3211..4263
                     /locus_tag="PLB85_03880"
     CDS             3211..4263
                     /locus_tag="PLB85_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOU72500.1"
                     /translation="MVTHKVQKGDTLYSLSKKYQVSIEEIWELNGLTSNNIGVGQVLK
                     IKKAEPVKPDFKTPPVKPAQTETSPSPPPAEPPDHSKLNLPDDYYYTVQAGETAFRIA
                     VNHKLTPSDFLRWNNLPAENPPIKPGDRLIIKDPASFNPAEEKPAEQGATIQAATPAP
                     ADTLLLDQVYVVKRKDTLFSIAKAHGTTVDDIKARNNLSSNDISVGQKLWLTGTPPAD
                     QASGSTSKVRTDCIMPTNGRVTSEFGMRRGRPHKGIDIANKSGTPIYAVLDGVVVYSG
                     TQRGYGNVVLIEHPNFVMTVYAHNEKNLVNVGDHVSQGQQIARMGSTGNSTGPHLHFE
                     YRVKGTALNPRKVLPL"
     gene            4286..>4648
                     /locus_tag="PLB85_03885"
     CDS             4286..>4648
                     /locus_tag="PLB85_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU72501.1"
                     /translation="MNRENVFADQALQNYLKEISKFKALSREEEYELGVRAKAGDQDA
                     INQLVQANLKFVVKIAARYQNRGLSLSELISEGNIGLLKAIEKFDPEKDIKLISYAIW
                     WIKQRIMLAVSEKSSLIRV"
BASE COUNT         1175 a         1320 c         1232 g          921 t
ORIGIN      
        1 agggaatcga ctttcacctc caaatagtct gtgtgccgca atacaactgt ggggaccggc
       61 tccgcgccac tctcagcgat ctgctggctg gttccgtgaa tgttttgtcc atcggagtgg
      121 tgccagttgg cctaaccggt ttccgggaaa accttacaga acttaagccc ttcacagcgg
      181 agctcgcggc ggaaaccatt gccatcattg attctttcag gcagcagggc cagccagtcc
      241 aagccgcgga cgagctttac gtcatggcag gcctgtctgt tcctgacgaa agttattatg
      301 gggattttcc ccagttggaa aacgggatcg gcatgctccg cctcagcagg atgaatttcc
      361 gccggcgccg cagtgcctta atcaaagagc tggagaaggc cgggatgcct ttcgtgatgc
      421 tgacctcgcg ttcggcagaa tccacaatca aagaactggc cggcaggctg gaaccaaagc
      481 tggaaaaagc ctctgtgcgg gtgaaagcca tagccaacaa ctggttggga ggccaggtta
      541 gcgtgagcgg tctgctcgcg gctaaggaca ttctggaaca acacaatgcc accgggaacg
      601 aaggtctgat cgtgccttcc aatattttca accatgacgg gattaccctg gacgacgttt
      661 ctcagatcga gttccgtgac ctcttgggcc ggccactgct agtggtggac cagtatttcg
      721 aggattggga atggatctga ccaataacac aatcataaca atccgacctc aaaaacggga
      781 gaataaccta tgaaacaagc tgcactgata gttttggcac tcatcctgtt gctggcttcc
      841 tgcatgagtg aagccgacaa actggaaaag gcgaatcttg aactcggttc aaagaagaat
      901 ccgatcaaga tgtatttcgt tccctcgatg gaagcctcca ccgtggtcac cagcggagag
      961 gccatcgccg cctggttgga acaggaaacc ggatacagat tcaaagtggc cgtgcccacc
     1021 agttatgccg ctgtggtgga agccatcggc gcctatcagg ccgatgtggc ctggctgcca
     1081 actttcgcct acgtgttggc tgaggaaaaa tacgaggctg aagtgcagtt catgacagtc
     1141 cgcaacggcc tgaacaagta ttgtgggcaa ttcgtggccc gcgccgacag cggcatcgac
     1201 agcctgagcc aaatcgaggg aaaggtggtg gcatacaccg acgcggcttc aacctccggc
     1261 tacatctatc cttccgccat tcttaaacag cagggcatca atccagccag atacaccctc
     1321 gcaggaggcc atccgcaagc aatcacagca gtttacaacg ggacagcgga cgtagcctgc
     1381 acctactggt cgcccccaga tgccagcggc aaaccaatgg atgccaggga aaagttgctg
     1441 gagacctatc ctgatgtctt ttccaagctg aaaatagtgg ctctcaccga ttccattccc
     1501 aacgacacag tcactttccg gcgaggcatg cctgacgagg tgaaaacaaa gctggtgcaa
     1561 gctttggaga aattcgccgg caacaaggac ggacaggcca tcatgaaaga gctttacgat
     1621 gtagacggcc taacccgcgc caccgatgcc gattatgaca ttgtgcgcca gacccttaaa
     1681 gcgctggaca aagaccccag gcagtttctc aaagactgag gcgggatggc tcagctcgag
     1741 ataaaagacc ttcgcaaaac ctatgaccac aaggtctggg ctttggacaa agtcagcttc
     1801 gatgtccaaa agggtgattt catcatcctg ctgggcctct ccggcagcgg caagtcaacc
     1861 ctcctgcgct gcataaacag gctgatagag ccggatgggg gcgacgtcct ctacgaagga
     1921 aaatcggtgc gcgccctgtc accaactcag ttgcgaagat accgccgcag catcgccatg
     1981 gtctttcagc agttcaacct tgtaaaaaac ctcagcgtgc tcaccaatgt gctcacgggg
     2041 cggctgggat atcacggctt gctggacaaa tacagcgcct cggaaatcga cgcggcctac
     2101 cataatctgg accgggtggg cttgaaaaaa tatgccaagc ggcaggtgaa aagcctctcc
     2161 ggcggccagc agcaaagggt ggccatcgcc cgggccctga tgcaaaatcc ggctattgtc
     2221 ctggctgacg aacccgttgc cagcctcgat cccgccaccg ctgattccat catgcaatat
     2281 ctggctgaaa tcaattccga aggcatctcc gtgctctgct cgctgcattt cctttccctc
     2341 gcccgccgct acggcaaccg cgttgtcgcg ctcaaggacg ggctcaaggt ttatgagggc
     2401 ctgcccgtcg agatagacaa cgcccgcttc aaacaaatct acggccagga cgcggaagag
     2461 gtctaagctc cctttcaggc aaactcttcc gccatcctga ccccagttcc ggcgcaagcc
     2521 tgataaattc cctaagccgg aataagcccg cttcccaaaa acctgatccg tccttagccc
     2581 catcacattc gccccttgcc tgcctccctc aaggctcccg cattccagac gggaggtgtg
     2641 cgggaggccc aaaataggcg gacaggatgg ggctaaggac ggaatcatca ggtaatcaac
     2701 tgacttccaa gcccaaacct acagaaagtg ggcaacaacc attgctaaac ggaactgcca
     2761 aaaacctaaa aacgaatcct tgacaaatat ccccagttga aatacaaagt ttttttcctt
     2821 aaatggagta gaatatgctt acttgggaac tacgagccac ggggcgcgtc caattcgtcg
     2881 gtttccgcag attcgcgcgg acttgcgctc tgcggtgctc gcttactggc tgggttcgca
     2941 atcagggtga cggcagcgtg ctgatcgaag ccaccgggga cagggacaag cttgagctat
     3001 tctgcgagct gctgcgtgcc ggcaacaggt ttgttgatgt gcgcgagctg cgcatcaggg
     3061 aacaagacga aacggttgtt tacgatgatt ttacgatacg ataaaaagac ccgaagcggg
     3121 acaggcggtc cgtgccatcg ccgcgggcct gcatggaagc tgtccattgt ggtgttggcc
     3181 ttactgctgc cggtttggtt ggcggcggag atggtcactc acaaggtgca aaaaggtgac
     3241 actctctact cgctgagcaa gaaataccag gtgtctatcg aagagatttg ggaattgaac
     3301 ggccttacca gcaacaatat cggtgtgggg caggttttga agattaaaaa agctgagccc
     3361 gtcaaaccgg atttcaaaac tccccccgtc aagcccgccc agacagaaac ctctccttct
     3421 ccgccgccag cggaaccgcc cgaccatagc aaactcaacc ttccagacga ttactactac
     3481 actgtgcagg cgggcgaaac agctttccgc attgccgtaa accacaaact gacaccaagt
     3541 gattttctac gctggaacaa ccttccagct gaaaacccgc cgataaaacc gggtgatcgc
     3601 cttatcatca aggacccggc aagttttaac cccgcggagg agaagccggc ggaacaggga
     3661 gccacaatcc aggccgcgac gcctgccccg gccgacacat tgctgctgga tcaggtttac
     3721 gtggtgaaga gaaaagacac cttgttcagc atcgccaagg cccacggaac caccgtggac
     3781 gacatcaaag cccgcaacaa cctctcttcc aacgacatca gcgtgggcca gaaactctgg
     3841 ttgaccggaa ctccgcctgc cgatcaggcc tccggctcaa cctctaaggt ccgcactgat
     3901 tgcatcatgc caactaatgg cagggtaacc tcggaatttg gcatgcgccg tggccgtccc
     3961 cacaagggca tcgacatcgc caacaaatcc ggaacgccga tctacgcggt tttggacgga
     4021 gtggttgtct attccgggac tcagcgcggc tacgggaatg ttgtcctgat cgagcatccg
     4081 aactttgtga tgacagttta cgcccacaac gaaaagaact tggtgaacgt gggcgaccac
     4141 gtgagccagg gtcagcaaat cgccagaatg ggctccaccg gaaattccac cggcccccat
     4201 ctgcactttg aataccgtgt caaggggacg gctctcaatc cccgcaaggt gttgccgtta
     4261 taatgtttaa ggcaagcggg tcctaatgaa ccgggaaaat gtttttgcgg accaggcgct
     4321 gcaaaattac ctgaaggaaa tatccaagtt taaggccctg agccgcgagg aggaatacga
     4381 actgggcgtc cgggccaaag ccggtgacca ggatgccata aaccagttgg tgcaggcgaa
     4441 cctcaaattc gtagtcaaga tagccgcccg ctaccaaaac aggggccttt ccctttccga
     4501 actgatcagc gaaggcaaca tcggcctgct gaaagcgatc gagaaattcg atccggaaaa
     4561 agacatcaaa ctaatatcct acgccatctg gtggataaaa cagcgcatca tgctggccgt
     4621 ttcggaaaaa agctccctga tccgcgtg
//
LOCUS       DAOOWR010000115         4619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18686_length_4619_cov_2.788563, whole genome shotgun sequence.
ACCESSION   DAOOWR010000115 DAOOWR010000000
VERSION     DAOOWR010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18686_length_4619_cov_2.788563"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..2100)
                     /locus_tag="PLB85_03890"
     CDS             complement(175..2100)
                     /locus_tag="PLB85_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72502.1"
                     /translation="MDKATPSKTRAWPNLRQIYAIMFRYWPLLVSGLLATLLYAIFSG
                     VSVTLVIPLFDYVFRPGGGNPVYTDLGGFLGAVGSAWGSFWAGAGGLFSLRGLKSLAP
                     LWEELQKVMLDTEPLVLLYALCLFVVAVILLKNIFFYLQRVLFIKLRGNTLRDLREAM
                     FSRYMGQSLEFFNQSRIGDAMVRMVNDAEIVSESFIKSLLEGILDIFTILVFTRVALL
                     LSPRLFLYGLLVVPFFTLFVGWLGKQVKRNSQRIQAHLSTMFSAVEEALNSMKVVKAF
                     RREESEHRAFSRVNSAYVRFWKRAQYYSSLSVPLSELNTVFTAVIVIILGGKMILAPG
                     SGFTLGDFTAFLFAIFSLLHPMKNLTQLYSDVKKALVSLNRVFHVLDSETEIKDAPEA
                     FAKESFTSHIEFDRVSFAYSEDRPVLSDVSLIISKGEKVAIVGASGGGKTTLANLFNR
                     MYDVSSGAILLDGTDIRQIRLADLRRLFGVVTQDSVLFTRSIRENIAFGSLEELGDER
                     IRAAARIAHAEDFILQFPRQYDEVLQTKGANLSGGQKQRLCIARAIVGDPPILILDEA
                     TSALDTESEQLVRQAIDAATQNRTVIMIAHRLSTVLQADRIVVLEQGQIVGQGSHEEL
                     LRSCPRYQKLYAAQYQE"
     gene            complement(2146..2222)
                     /locus_tag="PLB85_03895"
     tRNA            complement(2146..2222)
                     /locus_tag="PLB85_03895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2186..2188),aa:Arg,seq:acg)
     gene            2354..3031
                     /locus_tag="PLB85_03900"
     CDS             2354..3031
                     /locus_tag="PLB85_03900"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOU72503.1"
                     /translation="MKMRFLGHGTFDFVSEAGHKVLIDPFLDNNPQAALKSDAVSPDF
                     IILTHAHEDHFGDAIKIGKRCDPLFICMVELAKYLAEEGFKTHQIQIGGSHPFPFGRV
                     KLTPAWHSSTTPDGRYGGQAAGVLLWIDGKCVYHPGDTGLFYDMKLIGEMNRVDYFLV
                     PIGDNFTMGPEDALKAVEFVNPGMVIPMHYNTWPVINADPEDFTAKVQAMGYKCQILK
                     PGEELQA"
     gene            complement(3473..3754)
                     /locus_tag="PLB85_03905"
     CDS             complement(3473..3754)
                     /locus_tag="PLB85_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72504.1"
                     /translation="MSGSILKKLLPAFIPMAWEVVRHFKRDLNHDINIKKINETEEKL
                     ATIENLIVRMEKKTQLNREEIRTFKNHFYIWAAINSALLIAVFVKLFFF"
     gene            complement(3768..>4619)
                     /locus_tag="PLB85_03910"
     CDS             complement(3768..>4619)
                     /locus_tag="PLB85_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72505.1"
                     /translation="ERIMKKLIFIALILIQTALLLAVFDDYIPSARARGMGGAYTGVA
                     DDVNSLYFNPAGLTNVKLEAQVGFSRLYGNAFSEYKTAALGVKLPKKLGTLAFGARLF
                     DVAFEEETLMSEQVWTAGHAFDLQSDIHSQISVGYSVNYHRLQFIEEDSDGAIGVDFG
                     ATAFLHGRTKLGFSVCNLNQAVMGDANQHPLPSKLALGISYIPYENVITTLEVKKDFA
                     KETEFMGGVEVRPFEPLAVRFGVHSNPATWNAGIGLNVKGIELDLAYTNHAVLPGTLY
                     GNLGYKF"
BASE COUNT         1040 a         1216 c         1401 g          962 t
ORIGIN      
        1 gcggatggtc cagataatag tgaatctcct cccgggtgag ggcggaagac agccagcgcg
       61 tgacgcccct gcgcgcggcc tcttccaaaa ggggttccag gggcatcagc aggctaaggt
      121 tggccaggtg gcaatggccg tcaacaaggg tctgggtcgg aaaggtttgc gggctcattc
      181 ctgatattgg gcggcgtaga gtttctgata acgggggcag gagcgcaaaa gctcctcgtg
      241 gctgccttgg ccgactatct ggccttgttc gagaaccacg atcctatcag cctggagcac
      301 cgtggagagg cggtgggcga tcataatcac ggtgcggttt tgggtggcgg cgtcaattgc
      361 ctgccgcaca agctgttcgc tttcggtgtc gagggcgctg gtggcttcgt cgaggatcag
      421 gatgggggga tcgccaacga tggcgcgggc gatgcagagg cgctgtttct ggccgccgga
      481 gaggttggcg cccttggttt gcagcacctc gtcgtactgg cggggaaact ggaggatgaa
      541 atcttccgcg tgggcgatcc tggccgcggc gcgaatccgc tcgtcgccaa gctcctccag
      601 tgaaccgaag gcgatgtttt cccggatgga gcgggtgaag aggacgctgt cctgagttac
      661 caccccgaag aggcggcgga ggtcggccag cctgatttgg cggatgtccg ttccgtccag
      721 gaggatggcg ccggagctga cgtcgtacat gcggttgaag agattggcca aagtggtctt
      781 gccgccgccg ctggcgccca cgatggcgac cttttcgcct ttgctgatga tcaggctgac
      841 gtcgctgagc acggggcggt cttcgctata ggcaaagctc acgcggtcaa actctatatg
      901 gctcgtaaaa ctttccttgg caaaggcctc cggggcgtcc tttatctccg tttcgctatc
      961 cagaacatgg aaaacgcggt tgagcgagac cagggctttt ttcacgtcgg aatagagttg
     1021 ggtaaggttt ttcatggggt gaagcaggga aaagatggcg aagaggaagg cggtgaaatc
     1081 gcccagggta aagccggaac cgggggccag gatcatcttg ccgcccagga tgatgacgat
     1141 gacggcggtg aacacggtgt tcagctccga aagcggcacg ctgagcgagg aatagtattg
     1201 ggcgcgcttc cagaaacgca cgtatgcgga attgacccgg ctgaaggcgc ggtgttcact
     1261 ttcctctcgg cgaaaggctt tcacgacctt catgctgttc aaagcctcct ccaccgcgga
     1321 aaacatggtg gaaaggtggg cctggatgcg ctgggagttc cttttgacct gtttgcccag
     1381 ccagcccacg aagagggtga aaaagggcac caccaaaagc ccgtagagga aaagccgggg
     1441 actgagcagc agggccacgc gcgtgaaaac caggatggtg aagatatcca ggatgccttc
     1501 cagcaaggat ttgatgaagc tttcgctcac gatttccgcg tcgttcacca tacgcaccat
     1561 ggcgtcgccg atccggcttt ggttgaagaa ttccagggat tggcccatgt agcgggaaaa
     1621 catggcctca cgcaggtcgc ggagggtgtt tccgcgcagc ttgataaaaa gcacccgctg
     1681 gaggtagaaa aagatgtttt tgagcaggat aacggccacc acgaagaggc agagtgcgta
     1741 aagcagaacc aggggttccg tgtccagcat cactttttgc agctcttccc aaagcggagc
     1801 caggcttttc aggcccctca gggagaaaag gccgccggca ccagcccaga aagagcccca
     1861 agctgaaccg accgccccca ggaagccgcc gagatcggtg taaaccgggt ttccgccccc
     1921 gggcctgaaa acgtaatcga acaaaggaat aaccagggtg acgctgacac cgctgaagat
     1981 ggcgtagagc agcgtcgcca gcagcccgga taccaacagg ggccagtatc ggaacatgat
     2041 ggcgtaaatc tggcgcaggt ttggccaggc cctggttttg ctcggtgtcg ctttgtccat
     2101 agtcaggcca taaaaaaaag ccagcgaatg ctggcaagaa aaagatggca cgccctgagg
     2161 gattcgaacc cccaaccttc tgatccgtag tcagatgctc tatccaattg agccaagggc
     2221 gcagaataga acaaacattc gcgggcttgg gtttctgtca agaacaatat ccggcggtgg
     2281 gaaacggaat attttgcttg acttcaggac gtggatttgg aataattgca aaaaccgttc
     2341 atggaggccc aagatgaaaa tgagattttt aggacacggc accttcgatt ttgtcagcga
     2401 agccggacac aaagtgctta tcgacccctt tctggacaat aatccgcagg ctgcgctcaa
     2461 atccgatgcg gttagcccgg atttcatcat cctcacacac gcccacgaag accacttcgg
     2521 cgacgccatc aaaatcggca agcgctgcga cccgctcttc atctgcatgg tggaactggc
     2581 gaaatacctt gccgaagaag gctttaaaac ccaccagatc caaatcggcg gcagccaccc
     2641 ctttcccttt ggccgcgtga aactgacccc ggcctggcac agcagcacga ccccggacgg
     2701 acgttatggc gggcaagcgg ctggagtcct gctctggatc gacggcaaat gcgtgtatca
     2761 ccccggcgac accgggctct tttacgatat gaagctgatc ggcgaaatga accgggtgga
     2821 ctacttcctg gtgcccatcg gcgacaactt cacgatgggg ccggaagacg ccctgaaagc
     2881 ggtggaattc gtcaaccccg ggatggtaat ccccatgcac tacaatacct ggcccgtgat
     2941 aaacgcggac ccggaggact tcaccgccaa agtgcaggcc atgggttata aatgccagat
     3001 cctcaaaccc ggcgaagaac tccaggcctg attgacagga tgtgatacaa ggttcctttt
     3061 caagccggtg tcctggcgta cagcgaacac agtgagcatg gacggcagga tattgtagaa
     3121 atcacacgtt gctgtcgtcg atgctcgtcc ctcgctcgac gccagcgttt agccttgcga
     3181 tgccctttgg ccgttaaggc cttttcctat gtcccagaaa cgcctcccgc ataatgcccg
     3241 catttgatgc gggtatctgg cgggaggtat gggagggcga tgaaggcagg cgctaagggt
     3301 gcgactggca ggaggtcatt atgggcaaat agcttacgac tctccttatt aaggctccct
     3361 ttcaaaccgg gtggttattc cgaagggcgg cagaatgata gcctaaaaag ataataaggc
     3421 ccaaccgctc cggagggcgg ctgagccgta atagtcaagc tctggctgtg gttcagaaga
     3481 agaaaagctt cacgaaaacc gcgatcagca gggcggaatt gatcgccgcc cagatgtaga
     3541 agtggttttt gaaggttctg atttcctcac ggttcagttg ggtcttcttt tccattctca
     3601 cgatcaggtt ttcgatggtg gccagttttt cttccgtttc gttgatcttt ttgatgttta
     3661 tgtcgtggtt caggtcccgc ttgaagtgtc tcacaacttc ccaagccatg ggaatgaaag
     3721 cggggagcag ttttttcagg atcgatccgg acatggaagg ctccttatca gaatttgtag
     3781 ccaaggttgc cgtaaagggt tcccggcagc acggcatggt tggtgtaggc gaggtcaagc
     3841 tcgattccct tgacgttcaa gccaatgccg gcgttccagg tggcggggtt ggagtgcacg
     3901 ccgaaacgga cagccagagg ctcgaacggc cgcacttcca ctccgcccat gaattcggtt
     3961 tccttggcga aatctttctt cacttcaaga gtggtgataa cgttttcata gggaatgtag
     4021 gaaatcccca gggccagttt gctgggcagg ggatgctggt tcgcgtcgcc catcacagcc
     4081 tggttgaggt tgcacacgct gaagcccagc ttggtgcgtc catgcaggaa ggcggtagca
     4141 ccgaaatcga ccccgatggc gccgtcggaa tcttcctcga tgaattgcag gcgatggtag
     4201 ttcacgctgt agcccacgct gatctgggaa tggatgtcac tctgcaggtc aaaggcgtgg
     4261 ccggcggtcc agacctgttc actcatcagg gtttcctctt caaaagcgac atcgaagagg
     4321 cgggcgccga aagccagggt gcccagtttc ttgggcagct tcacgcccaa agcggctgtc
     4381 ttgtattccg aaaaggcatt gccgtagagg cggctgaagc caacctgggc ttccagcttc
     4441 acgttcgtca ggccggcggg attgaaatag agagagttca cgtcgtccgc cacaccggtg
     4501 taagctccac ccatgccacg ggcccgggcc gagggaatgt agtcatcgaa tacagccagc
     4561 agcaaagccg tctggatcag aatgagggcg atgaatatca gttttttcat gattctttc
//
LOCUS       DAOOWR010000116         4588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18873_length_4588_cov_5.008162, whole genome shotgun sequence.
ACCESSION   DAOOWR010000116 DAOOWR010000000
VERSION     DAOOWR010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18873_length_4588_cov_5.008162"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1831)
                     /locus_tag="PLB85_03915"
     CDS             complement(<1..1831)
                     /locus_tag="PLB85_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate phosphate dikinase"
                     /protein_id="HOU72506.1"
                     /translation="MDKIESQALASNLAQTQVKQIILSPDSLWLLERSASYYGIQQRT
                     KLFLEELHHPFANLDSALELMRQSIVGDLWFYLQLEESEHALELILAIFGNIFDRAEE
                     VRVKQLCLNEYLDFVAALAEQEKVTQTILDKALDLLEDCHARAPELFLAVSGQCFKTL
                     LKLSERLGRQDEAVALLRGIYEASVKLWRDKSSIADWHDKYADLLTLRKTELEQAIGK
                     KFFAACLRQIKAAKTLRDFADIPSHSDIASRHRDLLASFSRLPEKIHYLFYLLSLPTM
                     NALSDHLLWDLNRLLGDLHESLSAKEINLLIDSIFDNLAEFRDQHGSIVLDCVRTVGK
                     AVLDPAEPELARRCLRKTIDLGFIPPGEIKINRDWQIVADKNHIKNIRVWLELISIDP
                     VLCKDLLAALIINLTQGGIFISDTDLFQKDVSQFLNSDIKPLFVQVKQLLRLFPVFYH
                     EIGAEGEIRDLSTDIDEMSSRKDRLIHFVRKQVHTESNNTHINLLRQILNYWTDLDPG
                     HLKGLLPDDVALFVQTPDERTKAQHKAVKKFFRENKTGVDQLLGQSWQTVAPLFRQVK
                     KEDFSLRRLELLTRIYYMLLDKYQLDPYDIAKFLARFNWFDEKE"
     gene            complement(1954..>4588)
                     /gene="polA"
                     /locus_tag="PLB85_03920"
     CDS             complement(1954..>4588)
                     /gene="polA"
                     /locus_tag="PLB85_03920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU72507.1"
                     /translation="FLHFLELKRAENILISFDRKGPTFRHELSEDYKANRPPMPDELA
                     AQIEPVRRFFQLIGLEEISLDGYEADDVLATLGESFKDSYDIVFVTSDKDYSQLVEER
                     VTIFDPARDISYDREGVFSKFGVHPEQFVDYLALTGDASDNIPGVRGIGPKGAEKLLR
                     QYQTLDGIYAHLDEITGSTQKKLAENKESAYLSRQLATIIRDAPIPLPEIEKLGFKGA
                     NLTQAADFLSRYELNQLRRRIENRFAPEAGEPLEAAKEEELTQGDIFAEEPAGDESLS
                     ERQPSFKAILATPEILPLLWDALRRAELVSLDTETDSLEPVGAKLVGISLCVNADEAW
                     YLPLGHQMYDNLPLEDTLAALKQATAGKTILGHNLKYDLIVLRGHGWELANPLWDTML
                     AAYLQDPGTNQYSLDACAIAELGHTMIPISSLLDKKSKTSFDLVDVEAARDYSAEDAW
                     AVFRLQPVYAKRLSYSGLKELYEDIELPLVPVLQRMEENGVKIDTPVLREISRAINLE
                     LKDLTDDIYRLAGREFNLNSTQQLAALLFEEMKIPAKKKTKSGYSTDITVLEQLAEDY
                     EIADKLIQYRSLTKLESTYVGALPKLINPATGRIHSSFNQTVASTGRLSSSNPNLQNI
                     PIRTELGRSIRKAFIASDPDWAIMSADYSQIELRLLALFSRDAALLEAFRKGLDIHRQ
                     TASLITGKPLDQVSGEERRQAKVINFGLLYGMGQKKLSRELGLSLEDAKTMIRHYFER
                     FPSISAYINDSKAEARLHRYSQTIFGRKLYLKNINSGHQGLRAEAERVAVNMPIQGTA
                     ADLIKLAMIAIHKLIRDDPRIRMVLQVHDELVFEVRRDFLAEAEDLVRREMENALPQQ
                     YRQVVELKTEIASGDNWFEAH"
BASE COUNT          819 a         1252 c         1460 g         1057 t
ORIGIN      
        1 gctccttttc gtcgaaccag ttgaagcggg ccaggaattt ggcgatgtca tagggatcga
       61 gctggtattt atccagcagc atgtagtaga tgcgggtgag caattcaagg cggcggaggg
      121 aaaagtcctc ctttttcacc tggcggaaaa ggggcgccac cgtctgccag gactgcccca
      181 gcagttggtc aaccccggtc ttgttctccc ggaagaattt cttcaccgcc ttgtgctggg
      241 ccttggtgcg ctcatccggg gtttggacga agagggccac gtcgtccggc agcaggccct
      301 tcaggtggcc gggatcgagg tctgtccaat agttgaggat ttggcgcagg aggttgatgt
      361 gggtgttgtt gctttcagtg tgcacctgtt tgcgcacgaa atggatgagg cggtccttgc
      421 gggagctcat ttcgtcaatg tcggtgctca ggtcgcggat ttcgccctcg gcgccgatct
      481 cgtggtagaa aaccgggaag aggcgcagaa gctgcttcac ctgcacgaac aggggtttaa
      541 tgtcgctgtt caggaactgg gaaacgtcct tctggaagag gtcggtgtcc gaaatgaaga
      601 tgccgccctg ggtgaggttg atgatgagtg ccgccagcag gtccttgcag aggacggggt
      661 cgatgctgat cagttccagc cagacgcgga tgttcttgat gtggtttttg tcggccacga
      721 tctgccagtc ccggttgatc ttgatctcgc cgggcgggat gaagccgagg tcgattgtct
      781 ttctcaggca gcggcgggcg agttccggct cagcggggtc cagaacggct ttgccgacgg
      841 tgcggacgca atccagcacg atactgccgt gctggtcgcg gaattcggcc agattgtcga
      901 agatgctgtc aataagcagg tttatctctt tcgcggagag ggattcgtgc aggtctccca
      961 gcaggcggtt gaggtcccag agcagatggt cgctgagggc gttcatggtg ggcaggctca
     1021 ggaggtagaa gaggtaatgg atcttctccg gcaggcggga aaaggaggcc agcaggtcgc
     1081 ggtggcggga ggcaatgtct gaatgggacg ggatgtcggc gaaatcccgc agtgttttgg
     1141 cggctttgat ctgcctgagg caagccgcga agaatttctt gccgatggcc tgttccagtt
     1201 cggttttgcg cagggtcagc aggtcggcgt atttgtcgtg ccagtccgcg atggaagact
     1261 tgtccctcca gagtttcacc gaagcttcat agatgccgcg gagcagcgcc acggcctcgt
     1321 cctgccgtcc caggcgttcc gagagcttca gcagggtctt gaagcactgt ccggaaaccg
     1381 ccaggaagag ttccggagcc cgggcgtgac agtcttccag aaggtcgagg gctttgtcca
     1441 gaatggtctg cgtcaccttt tcctgttccg ccagggcggc gacgaaatcc agatactcgt
     1501 tcaggcagag ctgtttgacg cgaacttcct ccgcgcggtc aaagatgttg ccgaagatgg
     1561 ccaggatcag ctccagggcg tgttcgctct cctccagttg cagatagaac cagaggtcgc
     1621 ccacgatgct ctggcgcatc agttccaggg cggaatccag attggcgaag ggatggtgca
     1681 gctcttccag aaagagtttg gtgcgctgct ggatgccgta gtagctggcg ctgcgttcca
     1741 gcagccacag gctgtccggt gaaagaatta tctgttttac ctgggtctgg gcaaggttgc
     1801 tcgccagggc ctgggattcg atcttatcca attgcttcct caattaataa tgggtctgga
     1861 cggccgcgcg aaccggcatt gccggagggt tcacccttta tccgcgcgtt ttcggctact
     1921 taataattcc gcgcttaaat gtcaagccac tttttagtgg gcttcgaacc agttgtcgcc
     1981 gctggctatc tccgtcttca gctccaccac ctgccggtat tgttgcggca gggcgttttc
     2041 catctcacgg cgaaccaggt cctcggcttc ggccagaaaa tccctgcgca cctcgaagac
     2101 cagttcatcg tgcacctgca gcaccatgcg gatgcggggg tcgtccctta tcagcttgtg
     2161 gatggcgatc atggccagct tgatgaggtc ggcggcggtt ccctggatcg gcatgttcac
     2221 cgccacgcgt tccgcctctg cgcgcaggcc ctggtgtccg ctgttgatgt ttttcaggta
     2281 gagtttgcgc ccgaagatgg tctggctgta gcggtgcagc ctcgcttcgg ccttgctgtc
     2341 gttgatgtag gcgctgatgg aggggaagcg ttcaaagtaa tgcctgatca tggttttggc
     2401 atcttccagc gataacccca gctcgcgcga aagctttttc tggcccatgc cgtagaggag
     2461 gccgaaattg atcacctttg cctggcggcg ttcttcgccg ctcacctggt ccaaaggttt
     2521 cccggtgatg agggaggcgg tctggcggtg gatgtccagg ccttttcgga aggcttccag
     2581 caaagcggcg tcgcgggaaa agagagccag cagcctcagc tctatctggg aataatcggc
     2641 gctcatgatg gcccagtcgg ggtccgaggc gatgaaggcc ttgcggatgg agcgtcccag
     2701 ctcggtgcgg atggggatgt tttgcagatt ggggttcgag gatgagagcc ggccggtgga
     2761 ggccacggtc tggttgaaag aggagtggat gcgcccggtg gcaggattga tcagcttcgg
     2821 cagggctccc acataggtgg attccagctt ggtgaggctg cggtactgga tcagcttgtc
     2881 ggcaatttca tagtcttccg ccagttgttc cagcaccgtg atatcggtgg aatagccgct
     2941 tttggtcttc tttttggcgg gtatcttcat ctcctcgaaa agcagcgcgg ccagttgctg
     3001 ggtggaattg aggttgaatt cgcgcccggc caggcggtag atatcatccg taaggtcctt
     3061 tagttccaga ttgatggccc tcgaaatctc cctcagcacc ggggtgtcga ttttcacgcc
     3121 gttttcctcc atccgctgca acaccggcac caggggcagc tcgatgtcct cgtaaagctc
     3181 tttcaagccg ctgtagctga ggcgtttggc atagacgggc tgaagccgga acacggccca
     3241 ggcgtcctcg gcggagtagt cgcgggcggc ctcgacatcc acaaggtcga agctcgtttt
     3301 gctttttttg tccagcaggc tggagatggg tatcatcgtg tggccgagtt ccgcaatggc
     3361 gcaggcgtcc agggaatact ggttggtgcc ggggtcctgc agataggcgg ccagcatcgt
     3421 gtcccagagc gggttggcca gttcccatcc gtgtccgcgc agaacgatca ggtcatattt
     3481 gaggttgtgg ccgaggatgg ttttccccgc cgtggcctgt ttcagagcgg ccaaagtgtc
     3541 ttccaggggc agattgtcgt acatctggtg tcccaggggc aggtaccagg cctcgtccgc
     3601 attcacgcag agcgagattc ccaccagctt tgcccccacg ggctccaggg aatcggtttc
     3661 cgtatccagg ctcacgagct ccgcccggcg cagcgcgtcc caaaggaggg gcaggatttc
     3721 gggagtcgcg agtatcgcct tgaaagaggg ctgccgctcc gaaaggcttt catccccggc
     3781 gggctcttcg gcgaaaatgt ctccctgagt gagttcctcc tccttcgccg cctcaagcgg
     3841 ttccccggcc tccggggcaa agcggttttc gattctgcgc ctgagctggt tcagttcgta
     3901 gcggctcaga aagtccgccg cctgcgtcag gttggcgccc ttgaagccga gcttttcgat
     3961 ttccggcagc gggatggggg cgtcccggat tatcgttgcc aactgcctgg aaagataggc
     4021 gctttccttg ttttcagcca gctttttctg cgtcgagccg gtgatctcgt ccagatgcgc
     4081 gtagatgccg tccagggttt gatactgacg gagcagtttc tccgcgcctt tcggcccgat
     4141 gccccgcacg ccgggaatgt tgtcggaagc gtcacccgtc agcgccaggt aatccacaaa
     4201 ctgctccggg tgaaccccga atttggagaa aaccccctcg cggtcatagc tgatatccct
     4261 ggcggggtcg aagatggtca cccgctcttc cacaagctgc gaataatcct tgtccgaggt
     4321 cacgaaaacg atgtcgtagg aatccttgaa actctctccc agggtggcca ggacatcatc
     4381 cgcctcatag ccgtcgagcg agatttcctc caggccgata agctggaaaa accgccgcac
     4441 cggctcgatc tgtgccgcca gctcatccgg catcggcggc cggttcgcct tgtaatcctc
     4501 gctcagttcg tgccggaagg tcggcccctt gcggtcgaag ctgatcagga tgttctccgc
     4561 ccgcttgagt tccagaaagt gcagaaag
//
LOCUS       DAOOWR010000117         4588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18874_length_4588_cov_4.851754, whole genome shotgun sequence.
ACCESSION   DAOOWR010000117 DAOOWR010000000
VERSION     DAOOWR010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18874_length_4588_cov_4.851754"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..2786
                     /locus_tag="PLB85_03925"
     CDS             285..2786
                     /locus_tag="PLB85_03925"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOU72508.1"
                     /translation="MKEGFLPKNDYRSIFFTDQAEKCSDDIKTLFLRHLEYSLVKDTT
                     NVQPWDIYFALGLSLRDKLIERWLRTQYEYRKQDVKKVFYLSMEFLIGRLLGNTLVNL
                     EEYDNAYDLLKEYGYALEDIIELEPDMGLGNGGLGRLAACFMDSLATQAYPAYGYGIR
                     YEFGIFKQNIVRGYQEEEPDHWLKKGCPWEIKRPEITYRVRFRGKLVREEQPDGRVDY
                     RWVDTNDVMAVAWDIPIPGYKVGNVNNLRLWQATATDEFDFDYFNSGDYVRAVEKKNI
                     SENISKVLYPNDNFHLGKVLRLEQEYFFVSATLQDILQGWKQDHDSFAELPEKIAIQL
                     NDTHPALAIPELLRILIDEERLDFETAWDITRRCFSYTNHTILPEALEKWSVELFKEL
                     LPRHLELIYRINDHVLREVTRLYPGDLAKMRNLSIIEEAPAKNLRMAQLCLHGSHAVN
                     GVAKLHSQIIKDRIFPDFYGMYPERFQNKTNGITPRLWLKVCNPQLASLISEHIGEAW
                     VADLELLRGIERYADDPEFLDAFLEIKQINKNALCRHIFKVTGIRVSADSIFDAQIKR
                     LHEYKRQLLNVLGTIARYLRIKDNPQAYCVPRTVIFAGKAAPGYFLAKVLIKLINNLG
                     VVVNKDPDVADRLKVVFLPDYTVSLAEKIIPATDVSVQISTAGFEASGTGNMKFALNG
                     ALTLGTMDGANVEMAEEIGAENMFIFGLDAGEVSALKQSGYNPYYYYSKDPELRRAIE
                     AIASDMFSPKEKGIFDPIVRSLLDSGDPYCIMADFRSFLEASQRIDTLWQDRRAWAKK
                     AVLNVARSGKFSSDRAIREYANEIWDVKPVMLDLP"
     gene            2801..4441
                     /locus_tag="PLB85_03930"
     CDS             2801..4441
                     /locus_tag="PLB85_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU72509.1"
                     /translation="MRPAGPGKCLPLLALALLLPAFGPGQVVINEVCYDPAGADGGKE
                     WIELYNAGDTDVNLQGARLLSGGAEFREFFTFPHYVLRARRFVLVGGEQVSQAVFHVP
                     MTLQNGGAETDGVRYLGPDGFYTDTLLYDSPNSNNLPDDTGAAGTDFAPDVAEGNSLA
                     RRMDGYDTNNCAADFCEEAEPTPGLTNRVRADYALLHPQAWRDEGSWRFGLWVKNLSD
                     IGPALCAGLSVCLDGLKIAENTVSDLAAGDSLRMLYTLPVSDDLNHRVTAFLDLENDP
                     DPSNNSVALDLFPQNLSQPRLNELMFAPETGKQEWIELWVESLPTRGDFSIRDANGND
                     FSFSLPGGAGYFVLCAAPEQFLLHYPGCPADAVVGTSGWAALNNDGDSILLLNAEGDS
                     LDWMGYSSANLRAGVSLERQQNSGQQAFWRLSLDPSGSTPGRANSQSAQVPDFQGHIS
                     VQGSPCNPREGQKISLFHKLESPQNYVNCKVYDISGGLVRVLADNTPVPAEGKIDWDG
                     RDTAGKIVPRGRYFFAWESRPSEGQKALREQVTAVIFY"
BASE COUNT         1016 a         1468 c         1241 g          863 t
ORIGIN      
        1 aattagagtg gtcggagttc cttcatctca ccaaaagctg agatatagca tgacttctaa
       61 cgatattaac gttatcagta tcattcggtt atgaaaaaat ggattccaac cttctctgta
      121 atgacggtaa attggtgatc agggttaccc accataattg aacttgagcc ttcttttatt
      181 cttgcgacag ccgctatcgt ttacaaagcc ggaaaaatcc gcttgacacc aaaagagctt
      241 cccgacgatg gtcccgccag tgaaaaatca ccgctgagga tttgatgaaa gaaggtttcc
      301 tgcccaaaaa cgattatcgc tccatttttt tcaccgacca ggcggagaag tgcagtgacg
      361 acataaagac attattcctg aggcacctgg agtattccct ggttaaggat accaccaacg
      421 tgcagccttg ggacatctat ttcgccctgg gcctgagcct gcgcgacaag cttatcgaac
      481 gctggctgcg aacccagtat gaataccgca agcaggacgt gaagaaggtt ttctacctct
      541 cgatggaatt cctgatcggc cgcctgctgg gcaacaccct ggtcaacctg gaagaatacg
      601 acaacgccta cgacctgctg aaagaatacg gctacgcgct ggaagacatc atcgagctgg
      661 agccggacat gggcctcggt aatggcggcc tgggccggct ggccgcctgc ttcatggatt
      721 cgctggccac acaggcctat cccgcttatg gttacggcat ccgctatgaa ttcggcatct
      781 tcaagcagaa catcgtgcgg ggctatcagg aggaggaacc cgaccactgg ctgaaaaagg
      841 gctgcccctg ggagataaag cgcccggaaa tcacttacag ggtgcgtttc cgcggaaaac
      901 tggtcaggga ggaacagccg gacgggcgcg ttgactaccg ctgggtggac accaacgacg
      961 tgatggccgt ggcctgggac atccccatcc ccggctacaa ggtgggcaac gtgaacaacc
     1021 tgcgcctctg gcaggccacc gccaccgacg aatttgactt cgactacttc aacagcggcg
     1081 actacgtccg cgccgtggaa aaaaagaaca tctccgaaaa catctccaag gtgctctatc
     1141 ccaacgacaa cttccacctg ggcaaggtgt tgcgcctgga gcaggaatac tttttcgtga
     1201 gcgccaccct gcaggacatc ctccagggct ggaaacagga ccacgacagc ttcgcggagc
     1261 tgccggaaaa gatcgccata cagcttaacg acacccaccc cgccctggcc atcccggagc
     1321 tgctgcgcat cctcatcgac gaggaaaggc tggatttcga gaccgcctgg gacatcaccc
     1381 gccgctgctt cagctacacc aaccacacca tcctgcccga ggccctggaa aaatggagcg
     1441 tggaactctt caaggaactg ctgccccgcc atctggaact gatctaccgg atcaacgacc
     1501 atgtccttcg cgaggttacc cggctctatc ccggcgacct cgccaagatg cgcaacctca
     1561 gcatcataga ggaagcgccc gccaagaacc tgcgcatggc ccagctctgc ctgcacgggt
     1621 cccacgccgt gaatggagtg gccaagctcc attcccagat catcaaggac cgcatcttcc
     1681 ccgattttta cgggatgtat cccgaacgct tccagaacaa gaccaacggc atcacccccc
     1741 gcctctggct gaaagtgtgc aatccccagc tcgccagcct catcagcgaa cacatcggcg
     1801 aggcctgggt ggcggatttg gagctcctcc gcggcattga gcgctatgcc gacgaccccg
     1861 agttcctgga cgcctttctg gaaatcaagc aaatcaataa aaacgccctc tgccgacaca
     1921 tcttcaaggt caccggcatc cgggtttccg cggacagcat tttcgacgcc caaatcaaac
     1981 gcttgcacga gtataagcgc caactgctca acgtgttggg aaccatcgcc cgctacctgc
     2041 gcatcaagga caacccccag gcctactgcg tgcccaggac cgtgatcttc gccggaaagg
     2101 ccgccccggg ctacttcctg gcgaaagtcc tcatcaagct tatcaacaac ctgggcgtgg
     2161 tggtgaacaa ggacccggac gtcgccgacc gcctcaaggt ggtcttcctg cccgattaca
     2221 ccgtctccct ggccgaaaag atcatccccg ccactgatgt ttccgtgcag atatccaccg
     2281 ccggattcga agccagcggc accggcaaca tgaaattcgc ccttaacggc gccctcaccc
     2341 tcggcaccat ggacggcgcc aacgtggaaa tggccgagga gatcggggct gaaaacatgt
     2401 tcatcttcgg cctcgatgcc ggggaggtaa gcgccctcaa acaaagcggt tacaacccct
     2461 attactatta cagcaaagac cccgagctgc gccgcgccat cgaagccatc gcctcggata
     2521 tgttctcgcc caaagaaaaa ggcatcttcg accccatcgt ccgctccctg ctggattccg
     2581 gcgaccccta ctgtatcatg gcggatttcc gctccttcct ggaagcttcc cagcgcatcg
     2641 atacgctctg gcaggaccgc cgcgcctggg ccaaaaaagc cgtcctaaac gtcgcccgct
     2701 caggcaaatt ctcttccgac cgcgccatcc gcgaatacgc gaacgaaatc tgggacgtga
     2761 agccggtcat gctggacctg ccctgaaccc ggagcgaaag gtgagacccg ccggcccggg
     2821 aaaatgcctt ccgcttctcg cgctcgcgct tttgctgccg gccttcggcc ctggccaggt
     2881 ggttatcaac gaagtctgct acgaccccgc cggagccgat ggcggcaagg agtggattga
     2941 gctttacaac gccggcgaca cggacgtcaa ccttcagggg gccaggctgt taagcggcgg
     3001 tgccgaattc agggagtttt tcacttttcc ccactatgtc ctccgtgcgc gccgctttgt
     3061 gctggtggga ggcgagcagg tcagccaggc ggttttccac gttcccatga ctctccagaa
     3121 cggaggtgcg gaaaccgacg gggtccgcta cctcggcccg gatggatttt acaccgacac
     3181 actgctttac gattccccca acagcaataa tctgcccgac gacaccgggg cggcgggaac
     3241 agattttgcc ccggacgtgg cggaaggcaa ttccctggcc cggcgcatgg atggctatga
     3301 cacaaacaac tgcgcggcag atttttgcga ggaggcggag cctaccccgg gacttaccaa
     3361 ccgcgtccgc gccgattacg ccctcctgca tccgcaagcc tggcgggatg agggttcctg
     3421 gcgcttcgga ctctgggtga aaaacctctc cgacatcggc cccgcgctct gcgccggact
     3481 gagcgtctgc ctggacggcc tgaagatagc ggaaaataca gtttccgacc tcgctgccgg
     3541 ggattcgctg cggatgctct acaccctgcc cgtttccgat gacctgaacc accgggtcac
     3601 cgcattcctg gatctggaaa acgaccccga cccttccaac aattccgttg ccctggacct
     3661 ctttccgcag aacctcagcc agccgcggct caatgagttg atgttcgctc ccgagaccgg
     3721 aaagcaggaa tggatcgagc tttgggtgga atcgcttccc acccgggggg atttcagcat
     3781 tcgggacgcg aacggcaacg atttcagctt ttccctgccc gggggcgccg gttattttgt
     3841 gctctgcgcg gctccggagc agtttctgct tcattatccc ggctgccccg ccgacgccgt
     3901 ggtcggcacc agcggctggg ccgccctcaa caacgatggc gacagcatcc tgctgctgaa
     3961 cgcggaggga gacagcctcg actggatggg ctattccagc gcgaacctga gagcgggggt
     4021 ttcgctggaa cgccagcaga attcagggca gcaggccttt tggcgccttt cgctcgatcc
     4081 ctccggctcc acccccggcc gggccaacag ccagagcgcg caggtgcccg attttcaagg
     4141 ccatatcagc gtccagggca gcccctgcaa cccccgcgag ggccagaaaa taagcctgtt
     4201 ccataaactg gaaagccccc aaaactacgt caactgcaag gtttacgaca tcagcggcgg
     4261 cctggtgcgg gtcctggcag acaacacccc ggttcccgcg gaaggcaaaa tcgactggga
     4321 cggcagggac acggcgggca aaatcgtgcc tcggggaaga tatttcttcg cttgggaatc
     4381 ccgcccctcg gaagggcaaa aggcattgag ggaacaagtt acggcggtga tattctactg
     4441 aggggcgttc cctcggaagc cgtcccaaga gattcacctg ccagcccgct gaataacctg
     4501 tcagccaggc cctgagcgcc cgtttccatc ctcctcccac gcctctcgca caatccccgc
     4561 gtcaaatgcg ggcatcatgc gggagaca
//
LOCUS       DAOOWR010000118         4586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18888_length_4586_cov_3.349150, whole genome shotgun sequence.
ACCESSION   DAOOWR010000118 DAOOWR010000000
VERSION     DAOOWR010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18888_length_4586_cov_3.349150"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /gene="radA"
                     /locus_tag="PLB85_03935"
     CDS             <1..1305
                     /gene="radA"
                     /locus_tag="PLB85_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU72510.1"
                     /translation="ESTKWSGKCPSCGSWSTMKESTRVTGKASKGGASDLQAPKPERI
                     KDLKYSGETRSATGVREFDLVIGGGIVGGMLALIGGEPGIGKSTLMLQLSEWMGKQSK
                     KVLYVSGEESAEQIHLRSTRLNIGSENIWLLCTNDAELMLKAAEESRPDIMIVDSIQS
                     VSLPSLDSLPGSITQLRETSNRILRSAKILRMPVFMVGHVTKEGFVAGPKIIEHMVDT
                     VLYFEGEPRGQYKILRAVKNRFGPTNEIGIFEMTNLGLVEVPNPNHIFLSNDGEHNGT
                     AIGCVMEGTRSFIVEVQSLATTSNYGTPQRVVVGLEQKKLAILLAILEKNLALYLRSS
                     DVFINLAGGIRSSDPGLDLAILAAIISSLKDKPLPEKSVFIGEVGLNGEVRPVSQLDA
                     RVNEAVKLGYEKVFVSGHAKLRQKGKAIRIRDVRELYGALDK"
     gene            1398..3512
                     /locus_tag="PLB85_03940"
     CDS             1398..3512
                     /locus_tag="PLB85_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU72511.1"
                     /translation="MNSKFLLLVIIVTLCVLGLYQLTKAGRHAYPEFQFTEGQIAEYD
                     ILAPFDFPVYKTEEQVQEEYAAKLAQVGKPYWMSPDVEFESYSSLDRLFALIYEAADS
                     SDPGAVALSARQQGFLLDNPVLMLTGDKQRISSSHARIKAALEDLYKAGIYSGISGDS
                     IRVETESGTQQRSLARYFELAEAKRLILQKLEPSLALLVDKNASKLIQANLVLDTESY
                     EEQKKQIFDSIDRVSGMVKQNEVIIRLNQRLKQEDINKVNSLSLEYQARGERKSTLMQ
                     WLGFMGLLLFILVVVYAFNTHYWLSTAREEHGFAGAVVLNAGFVVIMLAALLTNDVLG
                     LPVSLIPLALAIIPAAVLLGFSVGYFYTVGAVLLLGPFINWDASGMTLLLLSTLITLV
                     LINRFQARHNFIKIWLFLFLSVNLINGALTMISYTGGDFLEKLQGLFRNTGYSLVSTT
                     ISVIGSLALVTYFERKWNRATKQVLMELQEFNHPLLKRLATDAVGTYHHSLMVGNLAE
                     RAAAAIGANASLARVGSYFHDIGKSVNPEIFTENNEDSAEFYTKFTPEESAEIIRDHV
                     KEGAVLAEKHRIPKEVVDIIWQHHGTSYIRYFLDAAQRQGKVEDLDAFRYPGPLPQSK
                     EAALVMLADVVESTSKSKSAATDAEIAKLIDDTIQRLIRDGQFDEAPITIKDLAIAKE
                     VMCPVLESVFRKRLEYPEEKQP"
     gene            3509..3970
                     /gene="ybeY"
                     /locus_tag="PLB85_03945"
     CDS             3509..3970
                     /gene="ybeY"
                     /locus_tag="PLB85_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOU72512.1"
                     /translation="MSPLTFTFENETDINVDPQPLRAVAEIICSEESPQGECEVGLII
                     CTDEQIRKYNQLYRGDDSVTDVLCFNGTEGLPGGITRGPDTVICDILIDINQLQRQKG
                     SKSINKELMEIFIHGLLHGFGYDHIRAGDSKQMKAKEEYYIKKMEGTQQRG"
     gene            3987..>4586
                     /locus_tag="PLB85_03950"
     CDS             3987..>4586
                     /locus_tag="PLB85_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOU72513.1"
                     /translation="MIPVIFILLLLSAFFSGSEIAYFSLSRIYLKKLENSKQASAKRI
                     LRLLRQPRRLLITVLLGNTFVNMGFSSLTALIALKIAKEYGFNTSLTVTIQVIASTLI
                     IITFGEIIPKLMGLATANDFARIVSLPLQLISYILYLPVWLIDKFSLLLSSKQGSDRH
                     IMSRLTTEEFHNLIQSEHSKHSLDENEKQMLVGLFRFREA"
BASE COUNT         1143 a         1247 c         1252 g          944 t
ORIGIN      
        1 gaatccacga agtggagtgg gaaatgcccc tcctgcggga gctggagcac aatgaaagag
       61 agcacgcggg ttacgggaaa agccagcaag ggcggggctt ccgaccttca agcgcccaaa
      121 ccggagcgga tcaaagacct caaatacagc ggcgaaaccc gcagcgccac gggagtgcgg
      181 gaattcgacc tggtgatcgg aggcgggatc gtgggcggaa tgctggccct gatcggcggt
      241 gagccgggca tcggcaaatc aaccctcatg cttcagcttt cggaatggat gggcaagcag
      301 agtaaaaaag ttctctacgt ttcgggcgag gaaagcgccg aacaaatcca tctgcgcagc
      361 accagactga acatcggcag cgagaacatc tggctgctct gcaccaacga cgcggaactg
      421 atgctcaaag cggcggagga aagccggccg gacatcatga tagtggattc catccagtcg
      481 gtgagcctgc caagcctgga ttcgcttccg ggcagcatca ctcagttgcg cgagaccagc
      541 aaccgcatcc tgcgcagcgc gaaaatccta aggatgccgg tgttcatggt cggccacgtg
      601 accaaggaag gctttgtggc ggggcccaaa atcatcgagc acatggtgga caccgtcctc
      661 tacttcgagg gtgaaccgcg cgggcaatac aagattttaa gggcggtgaa gaaccgcttt
      721 gggcccacca acgaaatcgg catcttcgag atgaccaacc tgggcctggt ggaggttccg
      781 aatccaaacc acatcttcct ctccaatgac ggcgagcaca acggcaccgc catcggctgc
      841 gtgatggagg gaacgcggtc cttcatcgtg gaagtccaat ccctggccac cacatcgaac
      901 tacgggacgc ctcagcgtgt ggtggtgggg ctggagcaga aaaagctggc catcctgctg
      961 gccattctgg aaaaaaacct tgccctgtat ctgaggtcca gcgacgtttt catcaacctc
     1021 gcaggaggca tccggtcttc cgatcccggc ctggatttgg ccatattggc ggcaataatc
     1081 tccagcctca aagacaagcc cctgcccgaa aaatcggtat tcatcgggga agtggggctg
     1141 aacggcgaag taaggccggt ttcgcagctt gacgcccggg ttaacgaggc ggtgaaactc
     1201 ggctatgaaa aagtgttcgt ttccggacac gcgaagctta ggcaaaaggg gaaggcgatc
     1261 agaatccgcg atgtgaggga actttacgga gcgctggaca aatgacggag aggcctaccc
     1321 gggcggcgga acgaacggtg tttcagactt ctggcgatgc cccttgacag aaataccggg
     1381 cagcgaaaac taatcccatg aactcaaagt tcctattact ggtcatcatc gtcacactct
     1441 gcgtcttggg gctttatcag cttaccaagg cgggcaggca cgcctacccg gaatttcagt
     1501 ttactgaagg gcagattgcg gaatacgata tcttggcacc cttcgacttt ccggtttaca
     1561 agaccgagga acaggtgcag gaagagtatg ccgccaaact ggcccaggtg ggaaagccat
     1621 actggatgag tccggacgtc gagtttgagt cctacagcag cctggatcgg ctgttcgccc
     1681 tcatatatga agcggccgac agttccgacc ccggagcagt ggccctcagc gccagacaac
     1741 agggtttcct actggacaat ccggtcctga tgctcactgg agacaagcag cgcatctctt
     1801 cttcccacgc caggattaaa gccgcgctcg aggatttgta caaggcgggc atttactcag
     1861 gcatcagcgg agacagcatc cgggtggaaa cagagagcgg cacgcagcag cgcagcctgg
     1921 cgcgctattt tgaactggcc gaggccaaac gcctcatcct gcaaaagctg gagccttccc
     1981 tggcactgct ggtggacaaa aacgccagca agctcatcca agccaatctg gtgctggaca
     2041 ctgaaagcta tgaggaacag aaaaaacaga tatttgattc catcgacagg gtgagtggca
     2101 tggtaaagca aaacgaggtg ataatccgcc tcaatcaaag gctgaagcag gaagacatca
     2161 acaaggtgaa ctccctttcc ctggagtacc aggcccgggg agagcgcaaa tccaccctga
     2221 tgcagtggct gggtttcatg gggctgctgc tcttcatctt ggttgtcgtt tacgccttca
     2281 atacgcatta ctggctttca accgcgcggg aagaacacgg cttcgccggg gcggttgtcc
     2341 tcaatgccgg ttttgtggtg ataatgctgg ctgccctgct cacgaacgac gttctgggcc
     2401 tgccggtgag cctgattccc ctggccttgg cgataatccc ggcagccgta ctgctggggt
     2461 tctctgtggg atatttctat accgtggggg ctgtcctttt gctggggccc ttcataaact
     2521 gggatgcttc cggcatgact ttgctgctgt tatccaccct gataaccctg gttttgataa
     2581 acagattcca ggcacgacac aatttcatca aaatctggct attccttttc ctttccgtga
     2641 acctcatcaa tggtgccctt accatgattt cctataccgg gggcgacttt ttggaaaaac
     2701 tgcaaggcct tttccggaat accggatact cgctggtttc cacaaccatc tcggtgatag
     2761 gcagcctggc cttggtcacc tattttgagc gcaaatggaa ccgggcgacc aaacaggtgc
     2821 tgatggaact gcaggaattc aaccatcccc tgcttaaaag gttggccacc gacgccgtgg
     2881 gaacatatca ccacagcctg atggtgggga acctggccga gcgggccgct gcagccattg
     2941 gcgccaacgc ctcactggcc agggtgggaa gttactttca cgacataggc aaatccgtga
     3001 atccggagat tttcacggag aacaatgaag attcggcaga attctacacc aagttcactc
     3061 cggaagagag cgccgaaatc attcgcgacc acgtgaagga aggggccgta ttggcggaaa
     3121 agcaccgcat acccaaagag gtggtagata tcatctggca gcatcacggc accagctaca
     3181 tccgttattt cctggacgcc gcccagcgcc aggggaaggt cgaggacctt gacgctttcc
     3241 gctatccggg ccccctgccc cagagcaagg aagccgcttt ggtgatgttg gcggacgtgg
     3301 tggaatcaac cagcaaatcc aaaagcgccg ccaccgacgc tgaaatcgcc aaactgatag
     3361 acgacaccat ccagcgcctt atcagggacg gccagttcga cgaagcgcct atcacgataa
     3421 aggaccttgc tatcgccaag gaagtgatgt gcccggtgct ggaaagcgtt ttccgcaaac
     3481 gcctggaata tcccgaagaa aaacaacctt gagccccctg accttcactt tcgaaaacga
     3541 gaccgatata aatgtggacc cccaaccact gcgggcggtg gcagaaatca tctgctctga
     3601 ggaatcgccc cagggtgaat gtgaagtcgg gctgataatc tgcactgatg aacaaatccg
     3661 caaatacaac cagctttacc ggggggacga ttccgtgaca gacgtgcttt gcttcaacgg
     3721 cacggaaggc cttcccggtg ggataacacg aggacccgac acggttatct gcgatattct
     3781 gattgacata aatcaactcc agcggcaaaa gggttccaaa agcataaaca aggaactgat
     3841 ggagattttt atccatggcc tgctgcacgg gtttggctat gaccacatcc gcgcgggaga
     3901 ctctaagcag atgaaagcaa aagaagaata ctacattaag aaaatggaag gtacacagca
     3961 acgtggatga tggtagtata ttacctttga ttccggtcat atttattctt ttgctgctct
     4021 cagccttttt ctcaggatcc gagatcgcct atttttccct ttcccgcatt tacctgaaga
     4081 agctggaaaa cagcaaacag gcaagcgcga aaagaatcct gagattgttg aggcagcccc
     4141 ggcggcttct gatcacggtt cttttgggca atacattcgt gaacatgggc ttttcgtctc
     4201 tgaccgccct catcgccctg aagattgcca aagaatatgg attcaatact tccctgacag
     4261 tcacgataca ggtgatagca tccaccctga tcatcatcac cttcggcgaa atcatcccca
     4321 agctgatggg tttggctaca gcgaatgatt ttgcccgaat cgtaagctta cccctccaac
     4381 tcattagtta catcctttat ctgcctgtat ggctgataga taaattcagc cttctgctct
     4441 ccagcaagca gggttctgac aggcacatca tgtccaggct gaccacggag gagtttcaca
     4501 acctgatcca gtccgagcat tccaagcaca gcctcgatga aaacgagaag cagatgctgg
     4561 tgggcctctt ccgcttccgg gaagcc
//
LOCUS       DAOOWR010000119         4568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_18989_length_4568_cov_3.600931, whole genome shotgun sequence.
ACCESSION   DAOOWR010000119 DAOOWR010000000
VERSION     DAOOWR010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18989_length_4568_cov_3.600931"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PLB85_03955"
     CDS             complement(<1..133)
                     /locus_tag="PLB85_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU72514.1"
                     /translation="MKLRPIEEHLVVKPDSAIAEKTVGGIIIPDTAKEKPQIAEVIAV
                     "
     gene            complement(268..894)
                     /gene="udk"
                     /locus_tag="PLB85_03960"
     CDS             complement(268..894)
                     /gene="udk"
                     /locus_tag="PLB85_03960"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU72515.1"
                     /translation="MSHKAHLILIGGGTCSGKTTIAKAIGRRIGSLKTVILSHDNYYR
                     DLSHLSLEERAKVNFDHPDSIDQDYLVHDLKLMLAGEAVNVPDYDFATHSRTEGKLCI
                     AGAEVIILEGIFALYYNELLNLSDLKIFLDSDSDIRLARRLQRDILQRGRDAESVLNQ
                     YLETVKPSHQAFIEPTKKNADIIVPGEKEFDRVLYLLNGYLQHVIGSR"
     gene            complement(912..1961)
                     /gene="wecB"
                     /locus_tag="PLB85_03965"
     CDS             complement(912..1961)
                     /gene="wecB"
                     /locus_tag="PLB85_03965"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOU72516.1"
                     /translation="MKIAQIVGARPQFVKLAPLSRLIRERHTEIIIHSGQHYDIKMND
                     VFFRDMQIPAPDHNLNIGSGSQGVQTAEILSALEAVLEKENPDWVIVYGDTNTTLAGA
                     LAAAKLGLRTAHVEACLRSFNRAMPEEINRVVADHLADLLLCPTPTAMENARREGLQD
                     KARLVGDIMTDSLKLGLELAEGFSDILADLSLQAGSFSLLTLHRPYNVDDPARLEHIL
                     KGLNSLGKTIVFPVHPRTRNVLAQLNPKNFSCLRFIEPLSYLDFISLMGASDMVLTDS
                     GGIQKESYIIGKPCVTLRSETEWTETVDTGWNLLLPVDSSSFPNAILDFQPPSERPDL
                     YGSDVSRKILDVLEQ"
     gene            2146..2991
                     /locus_tag="PLB85_03970"
     CDS             2146..2991
                     /locus_tag="PLB85_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU72517.1"
                     /translation="MDLTNLFSQYATPERMNVVLRVLLTLAIGIPLIVLLKKLTKRIV
                     TNRFSPQSELLIVRFVYYLSMLILLVSVLNEFGFKLSALLGAAGVVGIAIGFASQTSV
                     SNIISGIFLISEKPFVAGDFIEVAGIRGTVESIDLLSLKLKTPDNQFVRVPNETMIKT
                     EVINLTRFPSRRINIKVSVAYKEDLNKVCRLLEEIAASEPLALKEPAPLIAVDDFGPY
                     SVDFLFGVWGKTDDYIDLKSSLMIRIKETLNTNNVEIPFPHVSLYAGEASKPIIINNE
                     GSANT"
     gene            2988..3689
                     /locus_tag="PLB85_03975"
     CDS             2988..3689
                     /locus_tag="PLB85_03975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU72518.1"
                     /translation="MKKKHKIKAVIFDLDGTLLDSLQDIADSMNKALADSGLPEHPLE
                     AYKDFVGDGLFKLTERVLPASRRKKADIEAVAEKFWVNYEESWFLHTKTYPGILYLIQ
                     LCVSHKIKVAILSNKVHYFTKKMIRHYFRGAMVNLGKNPFGIYSGEQPDKPMKPDPTR
                     ALELAERLKCKPENIAFMGDSPVDVQTAKNAGMISIGAAWGFSGREALEKAGADYVFD
                     TAAEFATEIEKNPIN"
     gene            3689..4105
                     /locus_tag="PLB85_03980"
     CDS             3689..4105
                     /locus_tag="PLB85_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU72519.1"
                     /translation="MRSYRKELWFHTDKRREFVNITPQVEDCLRESGIREGLCLVNPM
                     HITASVFINDDEGGLHQDFERWLERLAPEKPHSQYAHNGYEDNADAHLKRQIMGREVV
                     VAVTEGKLDLGTWERIFYGEFDGMRRKRLLVKIIGE"
BASE COUNT         1124 a         1137 c         1236 g         1071 t
ORIGIN      
        1 caacggcaat cacttcggcg atctggggct tttccttggc ggtgtcggga atgatgatgc
       61 caccaactgt cttttcagcg atggcgctat ctggcttcac cacaagatgt tcttcaattg
      121 gtctgagttt catttggttc atccataatg tatttttcaa tctgcatata tgataacgcg
      181 agagacgtaa ttagcaaacc tcttgttcga gtgctaagat tttgtcaagt cctaatccga
      241 atcattgagc ccagccaggt gcatgattca tctggagccg atcacgtgct ggagatagcc
      301 gttgagcaag taaagcacgc ggtcgaattc cttttcgccg gggacgatga tgtcggcgtt
      361 cttttttgtt ggttcgatga aagcctggtg tgagggtttc acggtttcta gatactggtt
      421 gagcacgctt tcagcgtcgc gcccacgttg caagatgtcc ctctggagac gtcgggcgag
      481 gcggatgtcg gaatcagaat ccagaaatat cttaaggtcg gagaggttta gcagctcgtt
      541 gtaatagaga gcgaagatgc cttcgaggat gatcacctcg gctccggcga tgcagagctt
      601 gccctcggtg cggctgtggg tggcaaaatc gtagtccgga acgttgaccg cttcgccggc
      661 cagcatcagc ttgagatcgt ggaccaggta atcctggtcg atggagtcgg ggtgatcgaa
      721 attgaccttc gcgcgctctt ccaggctcag atgggaaagg tcgcggtaat agttgtcatg
      781 ggacagaata actgttttaa ggcttccgat acggcggccg atggctttgg cgatggttgt
      841 cttaccgctg caggtgccgc ctccgatcaa gatcagatgt gctttatgag acatgggtca
      901 ctccctggaa ttcattgctc cagcacatcg aggatttttc tcgaaacatc ggagccgtag
      961 aggtcgggac gctctgaagg gggctgaaaa tccaggatgg cattaggaaa agagctggaa
     1021 tccacgggca ggaggagatt ccagccggtg tccacggttt cggtccattc ggtttcggaa
     1081 cgcagggtca cacagggttt gccaatgatg taggactctt tctggatgcc gccggaatcg
     1141 gtcaggacca tgtccgacgc gcccatcagg ctgatgaagt ccagataaga gagaggctcg
     1201 atgaagcgca ggcaggaaaa gtttttggga ttaagctgcg ccaaaacgtt gcgggtgcgc
     1261 ggatgaactg gaaacacgat ggttttgccc agagagttca ggcctttcag gatgtgctcc
     1321 agacgggcgg gatcgtcgac attgtaagga cggtgcaggg ttagaagcga aaacgaacca
     1381 gcttggagcg aaagatcggc tagaatgtca gaaaagccct ccgccagttc cagacccagt
     1441 ttcaggctgt cggtcatgat atcgcccacc agacgggcct tgtcttgcag tccttcacgg
     1501 cgggcatttt ccatagccgt gggggtgggg cataaaagca ggtcagcaag atggtcggcc
     1561 accacgcggt tgatctcttc aggcatggcc cggttaaaac tgcgcagaca ggcttccaca
     1621 tgggctgtcc tcaacccgag tttggcagcg gccaaagcgc cggcaagggt ggtgttggta
     1681 tcaccgtaaa caattaccca atcggggttt tccttctcca aaactgcttc cagggcggat
     1741 aggatttccg cagtttgaac cccctggctg ccggagccaa tgttcagatt gtggtcaggt
     1801 gcgggtatct gcatgtcccg gaagaaaaca tcgttcatct tgatgtcgta atgctgacca
     1861 ctgtggatga ttatctcagt atggcgttct cggatcaagc gcgaaagcgg ggccaatttc
     1921 acaaattggg gcctggcgcc aacgatttgg gctattttca ttggttgctc cttggctggg
     1981 ggatggatgc aaagttctca aaagcggcct cgatgtcaag tttagtttgt ttttgtctgg
     2041 aaatggagta acctattaaa aaaagataga caaataacag cctttcaaaa tcgtggcttt
     2101 gtgaaattaa caaattgcac aaacaaagga gcctaaattc attagatgga cttaacaaac
     2161 ttattcagcc agtatgctac tccggaaagg atgaacgtgg ttttacgtgt tttgctcacc
     2221 ctagccatcg gcatacctct gatcgttttg ctgaagaaac tcaccaaacg gatcgtcacg
     2281 aacagatttt cgccccagag cgaacttctg atcgtgcgct ttgtgtatta tctcagcatg
     2341 ctgatcctgc tggtgagcgt cctgaacgaa tttggcttca agctttcagc tctactgggc
     2401 gcggctggtg tggttggcat agccatcggt tttgcctccc agaccagcgt gtcgaatatc
     2461 atcagcggaa tcttcctgat ctctgaaaag ccctttgtgg cgggtgactt tatcgaggtt
     2521 gcgggaatcc gcggaaccgt tgaaagcatc gaccttctct cgctgaagct gaaaaccccg
     2581 gacaaccagt tcgtgcgggt accaaatgag accatgatca agacagaggt aatcaacctc
     2641 acaaggtttc cctcgcgcag gatcaacatc aaggttagcg tggcctacaa ggaagacctg
     2701 aacaaggttt gcaggctgct ggaagaaatc gcggcatctg aaccgctggc cctgaaagag
     2761 ccggctccac tgatcgcggt ggatgatttt ggcccataca gcgttgattt tctttttggc
     2821 gtctggggca aaactgatga ttatattgat cttaaatctt ccctgatgat ccgcatcaag
     2881 gagactctaa acaccaacaa tgtcgaaatt cctttcccgc atgtatcgct ttacgcggga
     2941 gaggcatcca aacctataat tattaacaac gaaggaagtg ccaacacatg aaaaagaaac
     3001 acaaaatcaa agccgtaatc ttcgacctgg acggaaccct gttggacagt ctgcaagaca
     3061 tcgcagacag catgaacaag gccctggccg acagtggctt acccgaacac cctctggagg
     3121 catacaagga ttttgtgggc gatgggctgt ttaaactgac cgagagagtt ttacctgcgt
     3181 cacggcggaa aaaagccgac attgaagcgg tagcggaaaa attctgggtc aattatgagg
     3241 aaagctggtt tctgcacacc aagacctacc cgggaatcct gtatctgatc cagctctgcg
     3301 tttcccacaa gatcaaggta gccatacttt caaacaaggt ccactatttc accaaaaaga
     3361 tgatccgcca ttatttccgc ggcgcgatgg tcaatctggg caaaaacccg tttggcatct
     3421 acagcgggga gcagcctgac aaacccatga aaccggatcc cacccgcgct ctggaactgg
     3481 ctgaaaggct gaaatgcaaa cccgagaaca tcgccttcat gggcgattcc cccgtggatg
     3541 tgcaaacagc caaaaacgcc ggaatgatct ccatcggcgc agcctgggga ttcagcggcc
     3601 gggaagcgtt ggaaaaagcg ggcgcggact acgtgttcga caccgcggct gaatttgcca
     3661 ctgaaataga gaaaaatccg atcaactgat gcgcagctac cgcaaggaac tgtggttcca
     3721 caccgacaaa cgcagggagt ttgtgaatat caccccccaa gtggaggact gcctgcgcga
     3781 aagcggcatc agggaaggtt tgtgcctggt gaatcccatg catatcaccg cctccgtgtt
     3841 cataaatgat gacgaaggtg gcctgcatca ggattttgag agatggctgg aacgccttgc
     3901 ccccgaaaaa ccacactcgc aatatgccca caacggctat gaggacaatg ccgacgcgca
     3961 cctcaagcgc cagataatgg gtcgtgaggt ggtggtggcg gttacggagg gaaagctgga
     4021 cctcggcacc tgggaaagga tcttctatgg tgaattcgac ggcatgcgcc gcaagcgcct
     4081 gctggtgaaa atcatcggtg aataaataat gccccgccac ccggcggggc tttttttgct
     4141 ttcattgtaa ccaagccaat aaagactgga gggcaacaga ttttgaggga attctgtcgc
     4201 cctccaaact gctttctggt tgggcaccaa catgatataa aggagagtca tacccgctta
     4261 cccaccgcaa tatcctaccg gtctggctct tagcgctcgc ttccattcct ctcccacgcc
     4321 cctcgaacaa ttctcgtatc aaatgcgggc attgtgcggg aggcgtgttt gaaacacaag
     4381 acagggcctt aacggccaga ccacaccgct tcgttcttcc gaggagtgac aggtctcttt
     4441 acagtccctc tatcgcacac agttccagga cgttacctgc ccgatttgaa aaagagccgt
     4501 atttgcctgg cgctttggct gatggacctt ttcccttgac acatatcctc cccagcgcat
     4561 aatgttac
//
LOCUS       DAOOWR010000120         4540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19163_length_4540_cov_3.065775, whole genome shotgun sequence.
ACCESSION   DAOOWR010000120 DAOOWR010000000
VERSION     DAOOWR010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19163_length_4540_cov_3.065775"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLB85_03985"
     CDS             complement(<1..258)
                     /locus_tag="PLB85_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72520.1"
                     /translation="MKKIITLLMLSVCVFLAAQGQPEQQFMLPGDSLRVSHNDYKATL
                     DRLAPLVEKGIRNADLYYDLGVCHHHLGNEGQAVLNFLRALN"
     gene            complement(255..2087)
                     /locus_tag="PLB85_03990"
     CDS             complement(255..2087)
                     /locus_tag="PLB85_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOU72521.1"
                     /translation="MVVKKYLTVIFVLVCCAALTAQNVKVSATLRDGTIGLSDQMQLE
                     LKISSDKKLDMAAPAAPQVPGFSYRNVLSSTGVQSSFINGKFSSSHTKTFTYIYLPQK
                     KGMFKVPGFRLRIGGKDYATPDLSVTVEDSATTTKQPQQYSFDPYYDPFGGIYPDRSR
                     SQGETVLLCLPESQSVWLGEPAIISYYIYTDQRMDSFYSENENDYPGYGKSVYEQPQN
                     LNYEDVQYKGRRFQRALIKRSAIFPQTTGRLQMPTLTGKIQFSGFYSYMNRKVDSSPA
                     YINVKPLPAGKPAGFTGAVGKFSVNQVYSASKVTLGEAITCTVQVSGKGNFSQFTSPL
                     FPSVDKFQVSEPSVDDKLRNPLEGTRHINYTLLPRETGEFTLPSVTFSWFDTSSGTYK
                     TFRGQPQTVKVKQGNVLSYFSGLLEADAPKAMNPLLNRASHSDFRPYVYRTWFWLVLA
                     VLLFSLIVSGIISHNNRLSREDPAAYAIKTANRVLNKYWRQATEAASRLSPEFYPLAE
                     SGLSQYLAKKFGISRSLGTGELLNELWQKELPPRLIKQMEEFLLLCQEARYMPGGTEA
                     TDLHGALLKLRLLVQALSRLRFGNGKPRNRFSANTGNSLPEEKQ"
     gene            complement(2078..2716)
                     /locus_tag="PLB85_03995"
     CDS             complement(2078..2716)
                     /locus_tag="PLB85_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72522.1"
                     /translation="MKLPRLSLLAKLILALSLLILAFLLFELLFRPGVIRHALASASY
                     NRGNCRKAEKTWSGLRDPKDNDPIPENSLGKAQFRNGKYDESINSQEDALKEQDKVAQ
                     FHYDRGNALYRGKKLDEALKEYRSAMLLDPDDQDAKSNYELVLKRQGYKPPPPEPSPE
                     KQEDEEQRPAPKPPSEEDQEQYRNLLDALDQQEARNRQQKGHPSPKGSDKWW"
     gene            complement(2713..3687)
                     /locus_tag="PLB85_04000"
     CDS             complement(2713..3687)
                     /locus_tag="PLB85_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU72523.1"
                     /translation="MNVFYPQNLWLLLVLIPLVLLVTVLERRRRKRFSRFAEAQFYSE
                     YQKHHSPFFQALKAALTTLALVFIIFALLRPQWDYETRDYDSSGLDIMICLDISRSMD
                     ATDITPSRLERAKLQISALIDRLKGDRVGLISFAGVATMECPLTDDYESALLMLGGLN
                     TNSAARPGTDIGGALALAEKGFLGAGGSNVLLLITDGEDLGNNTLTQAQRLSSLGVKI
                     YTMGVGKEEGSLVRNPFSGEQILSKLDARRLREIAATGKGKYFSATPGQSEIDSILQD
                     VYSAEKGWGRSRHLTTLKDQYSLPAAAALCLLLAESFILPQRRTRKRK"
     gene            complement(3692..>4540)
                     /locus_tag="PLB85_04005"
     CDS             complement(3692..>4540)
                     /locus_tag="PLB85_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU72524.1"
                     /translation="KFWRYLFPVLRALTLLCLILALAQPRWGRGTRDITQKGVDIVLA
                     VDISGSMLALDFAPQNRLAAAVKVARDFISKRPNDRFGLVAFSEYALTQSPLTYDHIS
                     MLEQLGRLEVNQTASATAIGLGLAKSVARLKDSNAKSKVVILITDGVSNTGEIDPETA
                     ARMASSFKIKVYPIGVGTNGFVDFPITDPRFGTFYSKTFVELDMETLNRVAQITGTGK
                     AAQASDSDQLQQIMDNIDTLETTEYKTKVRYIWTEQFMLLLWTAFALLLLELILRLLV
                     MPVLPE"
BASE COUNT          947 a         1190 c         1292 g         1111 t
ORIGIN      
        1 gttcagggcg cgcaggaagt tgagcacggc ctggccttcg ttgcccaggt ggtggtgaca
       61 gacgcccaga tcataataca gatcggcgtt tcggatgcct ttttccacga gtggggccag
      121 cctgtcaaga gtggccttgt aatcattgtg ggaaacccgg agggaatcgc ccggaagcat
      181 aaactgctgt tccggttggc cttgcgctgc cagaaacacg cagacggaca gcatcagcag
      241 ggtaatgatt tttttcactg cttctcctcc ggcaagctgt tacctgtatt tgccgagaat
      301 ctgtttcggg gttttccgtt gccaaagcgc aggcggctga gggcctgaac cagcaggcga
      361 agcttcagca aagctccgtg caggtcggtc gcttcggttc caccgggcat gtagcgcgct
      421 tcctggcaca gcagcagaaa ttcttccatt tgcttgatca atcgtggagg aagttccttt
      481 tgccacaact cgttaaggag ttcgccggtg cccaggctgc gggaaatgcc gaatttcttg
      541 gccagatact gtgaaagtcc gctttcggca agtggataaa actctggcga aagcctggag
      601 gcggcttccg tggcctggcg ccaatacttg ttcaataccc ggttggcggt ttttatagca
      661 taagccgcag ggtcttccct gctcagacga ttgttgtggg atatgatgcc ggacacgatc
      721 agtgaaaaca gcagaactgc caaaaccagc cagaaccagg ttcggtaaac gtagggccga
      781 aaatcggaat ggctggctcg gttcagcagt gggttcatgg ctttcggggc atccgcttcg
      841 agcaatccgg agaaatatga cagcacgttg ccctgcttca ctttcaccgt ttgaggttgg
      901 ccccggaagg tcttgtaggt gccgctggaa gtgtcaaacc agctaaaggt tacgctgggc
      961 agggtgaatt ccccggtctc gcggggcagt agggtgtagt tgatatggcg tgtgccttcc
     1021 agcggattgc gcagtttgtc atccacgctt ggttccgaga cctggaattt atccacagag
     1081 ggaaaaagcg gcgaggtgaa ctggctgaaa ttgcctttgc cggatacttg gacggtgcag
     1141 gtgatcgcct cacccaaagt gacttttgag gctgaataga cctggtttac gctgaatttt
     1201 cccactgccc cggtaaatcc ggccggtttg ccggctggca agggtttcac gttaatatat
     1261 gccggcgagg aatccacctt gcggttcata tagctgtaaa atcccgaaaa ctggatcttg
     1321 ccagtgagag tgggcatttg caagcgtccc gtggtttggg ggaaaatggc cgaacgcttg
     1381 atcagagccc gctgaaagcg gcggcctttg tattgcacgt cctcataatt gagattctga
     1441 ggctgctcgt aaaccgattt gccatagccg ggataatcat tctcgttttc cgaatagaag
     1501 gaatccatcc gctgatcggt gtagatgtaa taggaaatga tagccggctc gcccagccag
     1561 acgctctggc tttccggcaa acagagaagc accgtctcac cctggctgcg gctgcggtct
     1621 ggataaatgc cgccaaaagg atcgtaatag gggtcaaaac tatactgctg aggctgttta
     1681 gtggtggtgg cggagtcttc gacggtaacg gaaaggtcag gcgtggcgta atcctttccc
     1741 ccgatccgga gcctgaagcc cgggaccttg aacatgcctt ttttttgagg cagatagatg
     1801 taggtgaagg ttttggtatg cgagctggaa aacttcccat tgatgaaaga actctgaact
     1861 ccagtgctgc ttagcacatt gcggtaactg aagccgggaa cctgtggggc ggcaggggca
     1921 gccatatcca gctttttatc gctgctgatc ttcagttcga gctgcatctg gtcggaaagc
     1981 ccaatggtgc cgtcgcgcag agtggctgag actttcacat tctgggctgt caaagccgcg
     2041 cagcagacga gcacaaatat taccgttaga tacttcttca ccaccattta tcagatcctt
     2101 tgggactcgg gtgccccttc tgctgtcggt ttcgggcttc ctgctggtcc agcgcgtcca
     2161 gcaggttgcg atactgctcc tggtcttcct cagagggtgg tttaggggct ggccgctgct
     2221 cttcgtcctc ctgcttttcc ggtgaaggct ctggaggggg cggtttgtag ccctggcgtt
     2281 ttaggaccag ttcatagttg gatttggcgt cctggtcgtc cgggtccagc agcatggccg
     2341 atctgtattc cttaagggcc tcatccagtt ttttgccccg gtagagggcg ttgccacggt
     2401 catagtgaaa ttgcgccacc ttgtcctgtt ctttcagcgc atcctcctgg ctgttaatgc
     2461 tctcgtcata tttgccgttg cggaattgcg ccttgccaag gctgttttcc gggatggggt
     2521 cattgtcctt gggatcgcgc aagcctgacc aggttttctc cgccttcctg caatttcccc
     2581 tgttgtaaga ggctgaagcc agggcgtggc ggatcactcc gggacggaac agcagttcga
     2641 aaagcaggaa cgccaggatt agcaggctta atgccaaaat cagctttgca agcaggctga
     2701 gacgcggcag cttcattttc gtttcctcgt tctgcgttga ggcaggatga aactttcagc
     2761 cagcagcagg catagtgccg ccgccgctgg aagggaatac tggtccttga gcgtggtcag
     2821 gtggcggctg cggccccaac ccttttcagc actgtaaacg tcttgaagga tgctgtcaat
     2881 ctcactctgc cccggagttg cggaaaagta tttccccttg cctgtggccg cgatttcgcg
     2941 cagcctgcgg gcatcgagct tggatagtat ctgctcgccg gagaaggggt tgcgcaccag
     3001 cgatccttct tcttttccca cgcccatggt atagatcttc actcccagag agctcagacg
     3061 ctgagcctgt gtgagggtgt tgttacccaa atcctcaccg tcggttatca acagcagcac
     3121 gttgctgccg cccgcgccca gaaagccttt ttccgccagg gccagcgccc cgcctatatc
     3181 cgttcccggg cgcgctgccg agttggtgtt caatccacca agcatcaaaa gggcgctttc
     3241 ataatcgtcc gtgagggggc attccatagt ggcgaccccg gcaaaggaaa tcaaacccac
     3301 cctgtctcct ttgagtctgt caatcaaagc gctgatctgc agtttggccc tctccagcct
     3361 tgagggtgtg atgtctgtgg cgtccatgct tctggaaata tccaggcaga tcatgatatc
     3421 cagcccgctg ctgtcgtaat ctcttgtttc gtaatcccat tgcgggcgca gcagcgcgaa
     3481 tataatgaag accagagcca gagtcgtcag cgcggctttt aatgcctgaa agaaaggtga
     3541 atggtgcttc tgatattcag aatagaactg cgcttcagcg aagcgggaaa aacgcttgcg
     3601 cctcctccgc tccaataccg tgaccaacag gactaggggt atcaatacca gcagcaacca
     3661 gagattttgg ggataaaata cgttcatctt ttcattccgg caatactggc ataaccaaaa
     3721 ggcgcagaat tagttccagc agcagcagag cgaacgccgt ccacaacagg agcatgaact
     3781 gctctgtcca tatgtagcgc accttcgttt tgtattctgt ggtttccaga gtatcgatat
     3841 tgtccatgat ctgttgcaac tggtcgctat cgctggcctg ggctgctttt ccggtgcctg
     3901 tgatctgtgc cacgcggttc agcgtttcca tgtccagttc cacaaaagtt ttgctgtaaa
     3961 aggttccaaa gcgtggatca gtgatgggaa aatccacaaa tccgttggtg ccgacgccga
     4021 tgggatacac cttgattttg aaagagcttg ccatcctggc tgcggtttcc ggatcgatct
     4081 caccggtgtt gctcaccccg tccgttatca ggatcaccac cttgcttttg gcgttgctgt
     4141 ctttcaggcg ggccactgac ttggccaggc caagcccgat ggctgttgca gaagccgttt
     4201 ggttcacctc cagtcggcca agctgttcca gcatagagat gtgatcatag gtgagcgggc
     4261 tctgcgtgag ggcgtattct gaaaaagcca ccaggccaaa cctgtcattg gggcgtttgg
     4321 agatgaagtc tcgcgccact ttcacggcag ccgccaatct gttttggggg gcaaaatcca
     4381 gcgccagcat ggaaccgctg atatccacag ccaaaacgat gtccacgcct ttctgggtga
     4441 tgtcccgagt tccgcgtccc cagcggggtt gggctaaagc caggatcagg cagagaaggg
     4501 tgagggcccg caacacggga aacaggtatc tccagaattt
//
LOCUS       DAOOWR010000121         4457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19608_length_4457_cov_3.650386, whole genome shotgun sequence.
ACCESSION   DAOOWR010000121 DAOOWR010000000
VERSION     DAOOWR010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19608_length_4457_cov_3.650386"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLB85_04010"
     CDS             <1..483
                     /locus_tag="PLB85_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72525.1"
                     /translation="ATALTVSDMVAATNTSQQQSYVFTDREINADGTYWYWLQNSDLD
                     GTVDYHGPISIAFTTVGGEIPSIPLVTALNPAYPNPFNPSTTLSYSLAEPSQVEIDIY
                     NQRGQIVRSYSKEHSAPGHYSFSFDGRDHSGVPLSSGVYLYRMRAGTFSQFRKMVLCK
                     "
     gene            complement(647..1930)
                     /locus_tag="PLB85_04015"
     CDS             complement(647..1930)
                     /locus_tag="PLB85_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU72526.1"
                     /translation="MKKVFELALLLALLLTLASCGSKKSPTGGPEDLDRPAILSSLPA
                     QFGQITNGRIEINFSKPLDKSSVTQAVYIYPPVRNKKISVDKSTLLIRFNEGLLPDTN
                     YYVTVSTRLKDIRGNALAENQTLVFARGRLNQLQLSGTVSFEEEEDASLPVQLRLLSA
                     DSLNVISTVSRGASYSLETLNPASYLLRAYIDKDRNNRHDPDREPFFSASVNLRHSQN
                     LDIALAYSDSTRPVMKLAQARHQREVEILFSEPLAGYDSVKLQHLDTGGDLPILITSL
                     AGDKLTLLTEVQEAQEYRVEVRGARDARGNVTPVDRADFRSSKIVDTTAPTVLSSSPR
                     NGTSMNTLEPTLVIRFSETIPASALKATLKAAESNAEIDFTLGGTNTDTYTFKPTRPL
                     QNYRSYVLTVTAADISGNKLAGEYKLNFLPLLRNR"
     gene            complement(1914..2540)
                     /locus_tag="PLB85_04020"
     CDS             complement(1914..2540)
                     /locus_tag="PLB85_04020"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOU72527.1"
                     /translation="MFTGIIQATARILRVVPSGGVRKVSIQRPAAFSELREGASIACD
                     GICLTVTGFNQASFEVEVMAETLAKSTAGAWNTNTLLNLEPALKLGERLDGHWVQGHI
                     DRVTKLLETSTKADTTRLRFSLDSRDRELLVPQGSIAVNGVSLTVSELGPSSFCVALI
                     SHTLKHTNLSQLVPGAAVNLEYDVLGKYVRALHQSGSLSLESLHEKGF"
     gene            complement(2545..3141)
                     /gene="recR"
                     /locus_tag="PLB85_04025"
     CDS             complement(2545..3141)
                     /gene="recR"
                     /locus_tag="PLB85_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU72528.1"
                     /translation="MLVSPALERLIQTLNRFPGIGRKTAQRLAWFLVSQNKDFALELA
                     ETITKTVETFTSCGLCNMLSESDPCPFCASAERSDALLCVVESTADIQIIENMNFYTG
                     RYFVLGHLLSPIEGFGPEQIRSGHLLARIKALRPEELVLALKPSPEGEATIHYLSELL
                     KDKGVQVTRLSTGIPFGGDLEYSSQLTLANAWKRRYPV"
     gene            complement(3142..3453)
                     /locus_tag="PLB85_04030"
     CDS             complement(3142..3453)
                     /locus_tag="PLB85_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOU72529.1"
                     /translation="MQNMNQLMKQAQKMQQEMLKSQQELESRVFEISSGGGVVKLEMT
                     GKFEVKSLTIDPEAVSPDDVEMLQDLILAALKEAHGQVSAAAEEIMGKVTGGMRIPGL
                     M"
     gene            complement(3567..>4457)
                     /locus_tag="PLB85_04035"
     CDS             complement(3567..>4457)
                     /locus_tag="PLB85_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72530.1"
                     /translation="GGSLDPGSASSLRLYPRMTFDNYDWEYFADASSAPSGTPVLQIS
                     RLNGVPDTHGVLAGQYDLSPLTPAWSLSVSGSSDFYSTQLPWLRFRQNSRAQNLLFSI
                     YEGEFYRIYPYSQSASADPWHFTFADGHVGFYLSSDAVYAPMIDVNPHLSVSTQVNSS
                     AADHIVSLYQAQLNLPANLIGSAVPLNSRIILREAVDYSVPHLSAYFLDFRTAAGTQY
                     DPGFYSLPGAPSLPYIYIPIPDYVPGQSHSLFFRSVDGTLTEFSLVDDFGENGLGEYI
                     MIGNCAVALVDGPGWFYTKN"
BASE COUNT          934 a         1146 c         1387 g          990 t
ORIGIN      
        1 gccaccgccc tcactgtcag cgatatggtg gcggccacca acacctccca gcagcagagc
       61 tacgtgttca ccgaccgcga gatcaacgcg gacggcacct actggtactg gctgcaaaac
      121 tccgatctgg acggaacagt ggactaccac ggccccattt caattgcctt cacgaccgtg
      181 ggtggcgaga ttccctccat tcctttggtc acggcgctca atccggccta ccccaacccc
      241 tttaacccca gcaccacgct gtcatacagc ctggcggaac cttcgcaggt ggagatcgac
      301 atctacaacc agcgcggcca gatcgtgcgt tcttattcca aggaacacag tgcccccggc
      361 cattacagct tcagctttga cggccgcgac catagcggag tgccgctctc cagcggggtt
      421 tatctctacc gcatgagagc aggaactttc tcccaattcc gtaaaatggt gctctgcaaa
      481 tagggcaagt cttctcataa aacactcctg aagcccggga cccatgttcc gggttttttt
      541 atttttgggc tgtcaaagca ggaggcaagc gtagcgagga ttgtcccttg acggttttcc
      601 gctcaacgga taatcaattt caagcttccg tgttatgaat gagcagctac cggtttctca
      661 gcaggggcag gaaattcagc ttgtattccc cggcgagttt gtttccgctg atatccgcgg
      721 cggtgactgt aagcacgtag gagcggtagt tttgcagggg gcgtgtgggt ttgaaggtgt
      781 aggtgtctgt attggtgccg ccaagggtaa aatcgatttc cgcgttggat tccgcggctt
      841 tcagcgtagc tttcagcgcc gaggcgggga tggtttcgga aaaacgtatt accagcgtgg
      901 gctcgagggt attcatagaa gtgccgttgc ggggcgagct ggacaaaact gtgggcgcgg
      961 tggtatccac tattttggag gagcggaaat cggccctgtc caccggagtg acgttgcccc
     1021 gggcgtcacg agcgccgcgg acctccacac ggtattcttg ggcttcctgg acctcggtga
     1081 gcaaggtgag tttgtccccg gcaaggctgg tgatcaggat gggaagatcg ccaccggtat
     1141 ccagatgctg cagtttaacg gagtcgtagc cggccagggg ttcggagaaa agtatctcca
     1201 cctcgcgctg atgcctggcc tgggcaagtt tcatcacagg tcgggtgctg tctgaataag
     1261 ccagagcgat atccaggttt tgggaatgcc gcaggttcac actggcggag aaaaagggct
     1321 ctctatccgg gtcgtggcgg ttgttgcggt ctttgtctat gtaagcgcgc agcagatagg
     1381 acgcgggatt gagggtttcc agggagtagg aggcgccccg cgaaacggtg ctgatcacgt
     1441 tcagcgaatc ggcggaaagt agccttagct gcaccggcag ggacgcgtcc tcttcctctt
     1501 caaaactgac tgtgccggaa agctggagtt ggttgagccg ccctctggca aaaacgaggg
     1561 tctggttttc cgccagcgcg ttgccgcgga tgtctttcag cctggtgctc acggtcacat
     1621 agtagttcgt gtccggcaaa agcccttcgt tgaagcggat gagcagggtg cttttgtcca
     1681 cgctgatttt cttgttcctc accgggggat agatgtaaac cgcctgggtg acagagcttt
     1741 tgtccagggg cttggaaaag ttgatttcga tcctgccgtt ggtgatctgg ccaaactgcg
     1801 cgggcaggga actgaggatg gcaggacggt ccaggtcttc tggaccgccg gtggggcttt
     1861 ttttgctgcc gcaggaagcc agggtcagca gcagcgccag tagcagggcg agttcaaaaa
     1921 cctttttcat ggaggctctc caggcttagt gagccgcttt gatgcagtgc gcggacgtat
     1981 ttgcccaaga cgtcgtattc aaggttgacg gcggctccgg gcaccagctg gctgaggtta
     2041 gtatgcttaa gggtgtggga aatcagggcc acacagaacg aggaggggcc aagttccgaa
     2101 acggtgaggc tgacgccatt gacggcgatt gagccttgcg ggacaagcag ttcgcggtcg
     2161 cgggaatcga gagaaaaacg cagcctggtg gtgtcggctt tggtgctggt ttccagaagc
     2221 ttggtgacgc ggtcgatatg gccctgaacc cagtgcccgt ccaggcgttc gccgagtttt
     2281 agcgcaggct ccagattgag cagggtgttg gtgttccaag ccccggcggt gcttttggcc
     2341 agggtttctg ccatcacctc cacttcgaag ctggcttggt tgaagccggt cacggttagg
     2401 cagatgccgt cgcaggcgat gcttgcgccc tcccggagtt ccgaaaacgc ggcaggacgc
     2461 tggatggaaa cctttctaac tccgccggag ggaaccacac ggaggattct ggcagtggcc
     2521 tggatgatgc ctgtgaacat gatctcagac agggtagcgc cgcttccagg cgttggcgag
     2581 ggtcagctgg ctgctgtatt ccaggtcgcc gccgaagggg atgccggtgg agaggcgggt
     2641 aacctgaacg cctttgtctt tcagcaattc ggagaggtaa tggattgtgg cctcgccttc
     2701 cggagagggt ttgagggcta aaaccagttc ctcggggcgc aaagccttga tcctggctag
     2761 cagatggccg gagcgaatct gctcggggcc gaatccttcg atgggggaga gcaaatgccc
     2821 cagcacaaaa tagcggcctg tgtaaaagtt catgttttcg atgatttgga tgtcggccgt
     2881 gctttccacc acgcagagga gggcatcaga acgctcagcg gaagcgcaga aagggcaggg
     2941 atcgctttcg gagagcatat tgcagagacc gcaactggtg aaggtttcca ccgttttggt
     3001 gatggtttcc gccagttcca gggcgaaatc cttgttttgg ctcaccagaa accaagccag
     3061 gcgctgcgcc gttttgcggc cgatgccggg aaagcggttc agggtctgga tgaggcgttc
     3121 cagagcgggg gaaacgagca tctacatcag cccgggaatt ctcattccgc cggttacctt
     3181 gcccatgatc tcttctgcgg ccgcggacac ctgtccgtga gcttccttaa gcgctgccag
     3241 gatgaggtcc tgcagcattt ccacatcgtc cgggctcacc gcttcgggat cgatggtgag
     3301 ggatttaacc tcgaatttgc cggtcatttc cagcttgacc acaccgccgc cgctgctgat
     3361 ctcgaagacc ctggattcca gctcctgctg ggatttcagc atctcctgct gcatcttctg
     3421 ggcttgtttc atcagttgat tcatgttttg catatctgat cctttgtcat tagtcattta
     3481 ttaaggccaa aaagaccacg aaaatcacga ttccccgcgg gctgaattta gtcaagcgtt
     3541 tagtggctga tcctgccgaa gctatctcag tttttggtat agaaccagcc ggggccatcc
     3601 accaaagcaa cggcgcagtt tccgatcatg atatactcac cgaggccatt ttcgccaaaa
     3661 tcgtccacca ggctgaattc cgtcaaggtt ccgtccacgc ttcggaagaa gaggctgtgg
     3721 ctttggccgg gaacgtaatc cgggatggga atgtagatgt agggcaggct gggggctcca
     3781 ggcagggagt aaaagcctgg atcatactgc gttccggcag cggtgcggaa atccaggaaa
     3841 taggcgctga ggtggggaac agaataatcc accgcctcgc ggagaatgat ccgtgaatta
     3901 aggggaacgg ctgatccaat caggtttgcg ggcagattca actgagcctg gtagagggaa
     3961 acgatgtggt ctgccgcgct ggaattgacc tgcgtggaaa cgctcagatg tggatttaca
     4021 tctatcatgg gagcgtaaac cgcgtcgctg gaaagataga agcccacgtg gccgtcggcg
     4081 aaggtgaagt gccagggatc agcgcttgct gattgagaat atggataaat cctgtaaaac
     4141 tcaccttcgt agatggagaa aagcaggttc tgcgcccggg agttttgccg gaagcggagc
     4201 cagggcaact gcgttgaata aaaatcgctg gagccgctca cggaaagcga ccaggctggc
     4261 gtcaacgggc tgaggtcata ttggccagcc agcacgccat gtgtgtcggg aacaccgttc
     4321 agacgggaga tttgcaaaac cggagtgcct gaaggtgcgc tgctggcgtc agcgaaatat
     4381 tcccaatcgt aattgtcgaa ggtcatgcgt ggatagagac gcaggctgct ggcggagccg
     4441 ggatcaagtg aaccgcc
//
LOCUS       DAOOWR010000122         4450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19647_length_4450_cov_4.294881, whole genome shotgun sequence.
ACCESSION   DAOOWR010000122 DAOOWR010000000
VERSION     DAOOWR010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19647_length_4450_cov_4.294881"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /gene="ftsH"
                     /locus_tag="PLB85_04040"
     CDS             complement(<1..2098)
                     /gene="ftsH"
                     /locus_tag="PLB85_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU72531.1"
                     /translation="MKHTVIIIALLALNLTLSAADSLSFPAQRARDVAQSNQVAQQFS
                     DLLLWFVIALVAITVISAVRIFLLKRKKPGERDSYKQPQTPPRTPRDLQPDQQLRQPA
                     RAPVSWTFVIVILALVALMVFSWFSGQEHIGEANYSQFAAALEKQQVKSVTFTEQDIV
                     YQTREGKKFHTLLPPVDDPSLIEQLRAQNVVVITKKPPRWTGILSYLIPFVLLIGFWW
                     FLMRGMNNQNARAFSFGKSRARMHEGSRSNITFKDVAGVDEAKEELQEIVEFLKDPKK
                     FQRLGGRIPRGVLLMGRPGTGKTLLAKAVSGEAGVPFYSISGSDFVEMFVGVGAARVR
                     DLFEQAKKNAPCITFIDEIDAVGRHRGTGLGGGHDEREQTLNQLLVEMDGFEPNEAVI
                     IIAATNRPDILDPALLRPGRFDRQVTVDLPDIKGRTEILKVHSAKVPLAEDVHLELIA
                     RGTPGFSGADLANIVNEAALIAARQNKQKINMSDFEEAKDKLTLGKEKKSRVIPDEDK
                     KLTSIHEIGHVLTSIFQDKTEPVHKVSIIPRGFTAGATHFLQTDKTGYSRGYLEQLMT
                     KILGGRAAEEIVFGELTTGAGNDLDRVTEIAKKMVCSWGMSEAFGPMTIGKEQSEVYL
                     GKELIGRDVHSNETAHLVDSEIRGFITRAYDKALDILKKRRDLLEKLAAELFEKETLG
                     TDEIFEFVLANIDDADR"
     gene            complement(2095..2640)
                     /gene="hpt"
                     /locus_tag="PLB85_04045"
     CDS             complement(2095..2640)
                     /gene="hpt"
                     /locus_tag="PLB85_04045"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU72532.1"
                     /translation="MNEMNRDLAEVLLDEYRIQARVREIGSQIASEYKDTVPVLIGIM
                     KGGFIFLADLCRSITIPLEIDFLAISSYGAETSSSGVVKIRKDIDVDITGRHVIVVED
                     IVDSGLSLQYIKDYLAKHEPASLKICVLLDKPAAHKQEVKFDYVGFEIGNEFVVGYGL
                     DFKSQYRNLPYIGILKEEIYS"
     gene            complement(2633..4066)
                     /gene="tilS"
                     /locus_tag="PLB85_04050"
     CDS             complement(2633..4066)
                     /gene="tilS"
                     /locus_tag="PLB85_04050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU72533.1"
                     /translation="MPKLKQALARLDSYVRNQQIFSAGAKLLLCVSGGADSVALLLLF
                     SRLRNLRQLTLLAVHVDHQLRGTESDADAELVKQHCQELSVPLIIRKICLEAGGDLEN
                     RARQKRLGVFEEVLDAYRFDLIVTAHHNNDQSETMLLNLFRGAGLSGLAGIRPRSGRI
                     AHPLLCFDKRELVELLEVRKLAWREDASNADQTFKRNWVRHTLLPLVERELNPRIASS
                     LGEQAQIFAAAEELVLQRVKTLVKRITLEQEPERITVSIPALKRCTRLEQYYVLKELT
                     SVLSGSGKDFFSRHFQDILDLLESDGSKQIQLGRGLIARKVYAELSLELESEAPPVPE
                     PVVVTEDRARAVWGDHRFTFKLLKVLPTRRDEDALNVYLDADKINWPFNIRSRRPGDR
                     FMPLGMKNLVKLKDFFINAKVGKFERDLVPVLDDGEKIIWIAGQRLDARVALDEGSTR
                     FLKIAAENLKAKPRRAANRKKTGEENE"
     gene            complement(4202..>4450)
                     /locus_tag="PLB85_04055"
     CDS             complement(4202..>4450)
                     /locus_tag="PLB85_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU72534.1"
                     /translation="PLHNIKEGTRYPDTMVLTADHDDRVVPGHSFKFAATMQDKADPD
                     GMAILYTQTSSAHGPSSLAKSLEYTADTYSFICMCLGV"
BASE COUNT          872 a         1332 c         1168 g         1078 t
ORIGIN      
        1 cccggtccgc gtcatcgatg ttggccagca cgaattcaaa aatctcgtcg gtgcccaggg
       61 tttccttctc aaaaagttcg gcagccagtt tttccaacaa atcgcgcctc tttttcaaga
      121 tgtccaatgc cttgtcatag gcgcgggtga tgaaaccgcg gatctcgctg tccaccaaat
      181 gcgccgtttc gttgctgtgc acatcgcggc cgataagttc cttgcccaga taaacttcac
      241 tctgctcctt gccgatggtc atcggaccga aggcttcgct catgccccag gagcagacca
      301 tctttttggc gatctccgta acccgatcca gatcgttgcc tgctccggtg gtgagttccc
      361 cgaaaactat ctcctccgcg gcgcgtccgc ccaggatctt ggtcataagc tgttccagat
      421 agccacgcga atagccggtc ttgtccgtct gcaagaagtg ggtagcgcct gctgtgaagc
      481 cgcgcgggat aatagaaacc ttgtgcaccg gctctgtctt gtcctggaaa atggaggtaa
      541 gtacatgccc gatctcgtgg atggaagtga gctttttatc ttcatcgggg attaccctgc
      601 tcttcttttc cttgcccaag gtcagtttgt ccttggcttc ctcgaaatcg ctcatattga
      661 ttttttgctt gttttgtctg gctgcgatca gcgccgcctc gttcacgata ttcgccaaat
      721 cggctccgct gaagcccggt gtcccgcgcg cgatcagctc cagatgcaca tcttcagcta
      781 gcggcacctt ggcagagtgc actttcagga tttcggtgcg gcctttgatg tcgggcaggt
      841 caaccgtcac ctggcggtcg aagcgtcccg gacgaagcaa cgcgggatcc aggatatcag
      901 gccggttagt ggcagcgatg atgatcacgg cttcgtttgg ctcaaagccg tccatctcca
      961 ccagcagttg gttcaacgtt tgttcgcgtt cgtcgtgacc gccgcccagc ccggttcccc
     1021 ggtgccggcc cacagcgtcg atttcatcaa taaaagtgat gcagggcgcg ttcttcttcg
     1081 cctgctcaaa aaggtcacgc accctcgcgg cgccgacgcc cacaaacatt tccacaaagt
     1141 ccgagccgct gatgctgtag aacggcaccc ccgcctcgcc ggaaacggcc ttggccagaa
     1201 gcgtcttgcc cgtcccagga cgtcccatca gcaatactcc gcgcgggatg cgtcctccca
     1261 gccgctggaa tttcttcggg tccttcagaa actccacgat ttcctgcagt tcctctttgg
     1321 cctcgtccac cccagccaca tccttgaaag tgatgttgct gcggctgcct tcatgcatgc
     1381 gggcccggct tttgccaaag ctgaaggccc tggcgttctg gttgttcatg ccccgcatca
     1441 ggaaccacca aaatccgatc agcagcacaa agggtatcag gtaggaaagg attcccgtcc
     1501 agcgcggagg ttttttggtt atcaccacta cattctgcgc ccgcaattgt tcgatcaggc
     1561 tgggatcgtc aaccggaggc agcagggtgt ggaatttctt gccttcccgg gtttggtaaa
     1621 cgatgtcctg ctctgtgaag gtcaccgatt ttacctgttg cttttccagc gcggcggcga
     1681 attgtgaata gtttgcctcg ccaatatgct cctgtccact gaaccagctg aacaccatca
     1741 gcgccacaag tgccaggatc actatcacaa aggtccagga aacaggagct ctggctggct
     1801 ggcgtagctg ctggtcaggc tgcaggtcgc gcggtgttcg tggcggcgtt tgcggctgtt
     1861 tgtagctgtc tctttcgccc ggttttttcc gcttcagcag aaagatgcgc accgcgctga
     1921 tcactgtgat ggccaccaag gctatcacaa accacagcag cagatctgaa aactgttgcg
     1981 ccacctggtt gctctgagcc acatccctgg ccctctgggc cggaaaggac aggctgtcgg
     2041 ctgctgacaa agtcagattc agcgccagta gcgctattat gatgactgta tgtttcatga
     2101 atagatctct tcctttagga tgccgatgta gggcaggttg cggtattggg atttgaaatc
     2161 cagcccgtat cccaccacaa attcattgcc gatttcgaag cccacatagt caaatttaac
     2221 ctcctgcttg tgcgccgcag gcttatccag caggacgcaa atcttcaggc tggcgggctc
     2281 gtgcttggct agataatctt taatgtattg cagcgaaagc ccgctatcca cgatgtcttc
     2341 tacaacgatc acgtgccggc cggtgatatc cacgtcgata tccttgcgaa tcttcaccac
     2401 tccggaactg gaggtctcgg cgccatagct agagatggcc agaaagtcga tttccagggg
     2461 gattgtgata ctgcggcaga ggtcggccag aaatatgaag ccgcctttca tgatgccgat
     2521 cagcaccggc accgtgtctt tatattcgct ggcgatttgg ctgccgattt cgcgcaccct
     2581 tgcctggatg cggtattcgt ccagcagcac ctctgccagg tcacgattca tctcattcat
     2641 tttcctctcc tgtctttttg cggttggccg cccgccgggg cttggccttc aggttttccg
     2701 ccgctatctt aagaaagcgc gtgctgcctt cgtccagcgc cacccgcgcg tccagacgct
     2761 gccccgcgat ccagattatc ttctccccgt catccagcac gggtaccaaa tcgcgctcaa
     2821 atttgcccac tttggcgttg atgaaaaagt ctttcagttt caccaggttt ttcataccca
     2881 gaggcatgaa cctgtcgcca ggcctgcggc tgcggatgtt gaagggccag ttgatcttgt
     2941 ccgcgtccag atagacgttc agggcgtctt catcgcgccg ggtcggcaat actttcagca
     3001 gcttgaaggt aaagcggtgg tctccccaaa ctgcccgggc ccggtcttcc gtcaccacca
     3061 ccggctcagg aaccggcggt gcctcgcttt caagctccaa actcaattca gcgtaaactt
     3121 ttctggctat caacccccgc cccagttgga tttgcttgct gccgtcagac tccagcaggt
     3181 ccaggatgtc ctggaagtgg cggctgaaaa aatccttccc gctaccgctg aggacgctgg
     3241 tgagctcttt caaaacgtaa tattgttcca atcgcgtgca gcgcttcaaa gccgggatgg
     3301 aaacggttat ccgctccggc tcctgctcca gcgtgatccg cttcaccagg gttttaaccc
     3361 tctgcagaac aagctcttcc gctgccgcga atatctgggc ctgttcgccc aaactgctgg
     3421 ctatccgcgg attcagttca cgctcaacca aaggcaacag cgtgtgccgc acccagttgc
     3481 gcttgaaagt ctggtcagcg ttgctggcgt cctcgcgcca ggcgagcttc cgcacctcca
     3541 gcaactccac cagttcccgc ttgtcaaagc agagcagcgg atgcgcgatc ctgccgctgc
     3601 gggggcggat tcccgccaga ccgcttaaac ccgcgccgcg gaagagattg agcagcatgg
     3661 tctcgctctg gtcgttgttg tggtgcgcgg ttacgatcaa atcgaagcgg taggcgtcca
     3721 gcacctcttc aaacaccccc aggcgctttt gccgcgcccg gttttccagg tcgccgccag
     3781 cctccaaaca tatcttgcgg atgatgaggg gaacgctcag ctcctggcaa tgctgcttta
     3841 caagttcagc gtccgcgtcg ctttccgtcc cgcgaagctg atggtccacg tgcacagcca
     3901 gcagtgtgag ctggcgcagg ttgcgcaacc gcgagaacag caatagtaga gccactgagt
     3961 ccgccccgcc agaaacgcag agcaatagct tcgccccggc ggaaaagatc tgctgattcc
     4021 gcacataact gtctaatctt gcaagggctt gcttcagctt gggcattagc gctcctgtcg
     4081 ctttattctt ggtaagttag ctttttacga ccgctttgct tgtcaagtcc ctttttctgt
     4141 ccgccgcatt gaaaaagggt ctacatattc tatggaccct ctctgaaaac tacaaattac
     4201 ctcacacccc caaacacatg cagataaagc tgtaggtatc cgcggtgtat tccaggcttt
     4261 tggcaaggct gctggggccg tgcgcggaag aggtttgtgt atagagtata gccatgccat
     4321 ccggatctgc cttgtcctgc atggtggcgg caaacttaaa ggaatggccg ggcaccacgc
     4381 ggtcgtcgtg atcggcggtc agaaccattg tatccggata acgtgttccc tctttgatgt
     4441 tgtgcaaggg
//
LOCUS       DAOOWR010000123         4449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19655_length_4449_cov_3.400091, whole genome shotgun sequence.
ACCESSION   DAOOWR010000123 DAOOWR010000000
VERSION     DAOOWR010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19655_length_4449_cov_3.400091"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLB85_04060"
     CDS             <1..971
                     /locus_tag="PLB85_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOU72535.1"
                     /translation="AREHIFNVVGDLLSGYPGLDGLHLDYIRYPNSSLGYHPISMSRY
                     NDVKAQQNLDWNEWRILQVTSLVETIRQLVDKISPGLILSAAVMPDPDSAARYYAQDW
                     KDWLERGLVDYVCPMNYSTDSVKFRESLDAADRTGLSEKIVMGVRAWNNNGDSLVPNG
                     LARNYNIMDVVERIGEIRKHNFAGIALFSYDGLLKDSALQHLSRVAYSDEIIANLSAL
                     DPLGESTARTRFAADVKVSTEGRLYSLELLVPLEGRWTLELRDPFNRVHYRRVRYYLK
                     GPNRDHWNGVLANGERIVPGTYLVSLFRDMDLFEYVIPVTFPGLTR"
     gene            968..2434
                     /locus_tag="PLB85_04065"
     CDS             968..2434
                     /locus_tag="PLB85_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU72536.1"
                     /translation="MNTDRSKQAGMLTTVEFIRFGLKSLVGILLARILVPAELGSYRQ
                     LFLIYSTFSTLLLLGIPQSVLYFLPKLRHIDSKREFISRTVNLVSALAFVFGLGIFLL
                     RGFIARLFNNPQLELLLILYAVYPLFMFITQIYSQIMLGLKQPRKTAVFTLFSVATDF
                     VLILGTALITKNLYYIVLALMVSALLQWGFAQFQLRHWLTKVSFDPGFYQEVFRYSLP
                     LGLSSIIGMLSIQLDKLVVSGFFTPAQYAVFSIGAMELPFISILNNSVNAILLPHISS
                     EPAKMTEVYRAAVRKNALIIFPFAVLGLIFATPLITFLYTETYLGSVPFFRVYLLNLP
                     LRVATYGIIFMALRKTRYIMINSLVTLGLNLVLNLVLVRLIGMMGAVIATVIVTWLSV
                     ALYLYWMRHKLGLDLTELFPLKALLKTLLVAGISALPAYAVFWIFGRGWAFQFVALFV
                     YLVTFVVLGKLSNAILPYDIEYAQKLWAQFKSRFVKAR"
     gene            2493..3827
                     /locus_tag="PLB85_04070"
     CDS             2493..3827
                     /locus_tag="PLB85_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72537.1"
                     /translation="MTSVSRHILYIAYYFPPLGGPAAMRSLKNVKYLAQLGYQVQVLT
                     VQPALIRFPKDPSLLKETPAGVKVWRAFCPDATWLFKLLWGLKLNSLVRFIQHKVLIP
                     DHEVLWLPFAKRILHRIVRRDPQISVAVISSGPPSSLSLGLLLKCRHRIPFICDFRDE
                     WTNNPERINIDFPATTQSRELVMESRVLSAAGGISYLSQLMRNNFCRSYPFLENKPSA
                     IIPNGFDDSDFEGLTASTDANVFRLVYCGSFYDRRQPSPLWQAILSLVEAKKLDPNKF
                     RVDIYGRNTRSFVLDRFASNPVLERIVNLHPFLGYRDSLQELLRATALLLYIPSGKNT
                     ESVLTGKIFDYLRTGKPVLAIVPPAGLAAELLTKAGIGYIADHQDLPGIAERLMELYW
                     LWENRALGELKVNHDYITQFSRPNLARKLAKLIDAVATGQPAGLPVHTGEEQ"
     gene            3824..>4449
                     /locus_tag="PLB85_04075"
     CDS             3824..>4449
                     /locus_tag="PLB85_04075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72538.1"
                     /translation="MKVLFIPSWLPYRDDPLAGKFFLDQALAIADAEVAEIFLLNWGQ
                     NEFQLAVRRPLSSLKKLARLIPKRRGLSQIAPRLTEIRVPHLSWTSLVAKGNIDSLIP
                     KIKLPEKPDLIHAQVTFPAGYVAMRLAEKHGLPYIITEHSGPFPFPEFLKRGAISPLI
                     TEPLKAAERIVAVSSHLKQEIRTKTGLEALVIPNPVNTDFFIPSANPRP"
BASE COUNT          952 a         1336 c         1106 g         1055 t
ORIGIN      
        1 aggcgcggga acacatattc aacgttgtcg gagacctgct cagcggctat cccgggctcg
       61 acggcctgca tcttgattac atccgctatc ccaattccag cctcggctat caccccattt
      121 ccatgagccg ctacaacgat gttaaagccc agcaaaacct ggactggaac gaatggcgca
      181 tcctgcaggt gacctccctg gtggaaacca tccgccagct cgtggataag atatctcccg
      241 gcctcatctt aagtgccgcc gtgatgccgg atccagacag cgccgcccgc tattacgccc
      301 aggactggaa agactggctg gaacggggtt tggtcgatta cgtctgccca atgaattact
      361 cgacagacag cgtcaaattc cgcgaaagcc tcgacgcagc cgacaggaca ggcttaagcg
      421 aaaagatcgt gatgggcgtc cgcgcctgga acaacaacgg agattcgctc gttccgaatg
      481 gcctggcccg caattacaac atcatggatg tagtggaaag gattggcgag atacgcaaac
      541 acaattttgc cggtatcgcc ctcttcagtt atgacggatt gctgaaggac agcgccctcc
      601 agcatctttc ccgggtggcc tattccgatg aaattatcgc taatctctcg gcgctggacc
      661 ctctgggtga aagcaccgcc agaacaaggt tcgcggcaga cgtgaaggtc agcaccgaag
      721 gccgactcta ctcgctggag ctgctggtgc cgctggaagg cagatggacg ctggaactta
      781 gggatccctt caaccgggtg cattaccgcc gcgtgcgtta ttatctgaaa gggccgaatc
      841 gggaccattg gaacggagtg ctggccaatg gcgagcgaat cgttcccggc acctatctgg
      901 tcagcctctt ccgggatatg gacctctttg aatatgtgat tccggttacc tttccggggt
      961 tgacgagatg aacacagacc gcagcaaaca ggccggaatg ctcactacag tggagttcat
     1021 ccgctttggg ctcaaatcgc ttgtgggcat cttacttgcc cggattttgg tgccggccga
     1081 gttgggttcc tatcgccagc ttttccttat ctacagcact ttttccactc tcctgctgct
     1141 gggaattccc cagagtgtgc tctattttct gcccaaactg cggcacatcg attccaagcg
     1201 cgaattcata agccgcacgg ttaatctagt gagcgcactg gccttcgttt ttggcctggg
     1261 catctttctg ctgcggggtt tcatcgcccg cctcttcaac aatccccagt tggaactgct
     1321 gctcatcctt tacgccgtct atccgctgtt catgttcatc acccagatct acagccagat
     1381 catgctgggc ctcaagcagc cgagaaagac agcggttttc accctcttca gcgtggcgac
     1441 cgattttgtg ctgatcctgg ggacggcttt gatcaccaaa aacctctatt acattgttct
     1501 ggccctgatg gtctccgctt tgctgcaatg gggcttcgcg cagttccagc tgcggcactg
     1561 gcttacaaag gtcagcttcg atcccggctt ttaccaagag gttttccggt attcgctgcc
     1621 cctgggcctg tcctccatca tcggcatgct ttcgatccag cttgataagc tggtggtctc
     1681 aggattcttc acgccagccc aatatgccgt gttctcgatc ggcgcgatgg aactgccctt
     1741 catctctatc ctgaacaact ctgtgaacgc catcctgctg ccgcacatca gttctgagcc
     1801 agccaaaatg accgaggtct atcgcgccgc ggtgcgcaaa aacgccctta tcatcttccc
     1861 ctttgccgtg ctgggactga ttttcgccac accgctcatc accttcctct atacggagac
     1921 ctaccttggc tcggtgcctt ttttccgggt ttacctgctc aatttgccgc tgcgcgtggc
     1981 cacctacggc atcatcttca tggccctgcg caaaacccgc tatataatga ttaattcact
     2041 tgtcacacta ggactgaacc tggtgctgaa ccttgttttg gtgcggctga ttgggatgat
     2101 gggagccgta atcgccaccg tgatagtcac ctggctttcc gtggccctct atctgtattg
     2161 gatgcggcac aagctggggc ttgacctgac agagcttttc ccgctcaaag ccctgctgaa
     2221 aaccttgctg gtggcaggca tttcagccct gccggcctac gctgtgttct ggatctttgg
     2281 caggggttgg gcttttcagt ttgtcgcgct atttgtgtat ctggtcacgt ttgttgtttt
     2341 gggaaaactc agcaacgcca tcttgcccta tgacattgaa tatgcgcaaa aactctgggc
     2401 ccagttcaaa tcccgttttg tgaaggcacg ctgatggaca tgtatgcggc cccatccagc
     2461 cggaataaag gctccgaggg agtttgaacg ccatgacatc agtttcccgg cacattcttt
     2521 acatcgccta ttatttccca cccctgggtg gcccggctgc aatgcgcagc cttaaaaacg
     2581 tcaaatacct ggcccagctt ggttatcagg tgcaggtgct aacagtgcag ccagccctca
     2641 tccgctttcc caaagaccca tccctgctga aagagacacc tgctggtgtg aaagtctgga
     2701 gagctttttg cccagacgcc acctggcttt tcaagctgct gtgggggctg aaactcaact
     2761 ccctggtccg cttcatccag cataaggtgc tcatccccga tcacgaggtt ctgtggctgc
     2821 cttttgcgaa aaggatttta caccgaatcg tccgccgcga tcctcagatc agcgtggccg
     2881 tgatctcctc cggaccgcct tcctccctgt ctttggggct gctgctgaaa tgccgccacc
     2941 gcatcccttt catctgcgat ttccgcgacg aatggaccaa caacccggaa cgcatcaaca
     3001 ttgatttccc agccaccacc caaagccgtg aactggtgat ggaatcgcgc gttctatcag
     3061 ccgccggcgg aatctcctac ctgagccagt tgatgcggaa caacttctgc cgcagctacc
     3121 cctttctgga aaacaaaccc tcggccatca tccccaacgg atttgacgac agcgatttcg
     3181 aggggctcac cgcttcaacc gatgccaatg tctttcgcct tgtctattgc ggcagttttt
     3241 acgaccgccg ccagccaagc cccctctggc aggcaatttt gagcctggtc gaagccaaaa
     3301 agctggaccc aaacaagttc cgggtggata tctacggtag aaacacccgc tccttcgtgc
     3361 tggataggtt cgcctcaaat cctgtcctgg agaggattgt gaaccttcat cccttcctcg
     3421 gttaccggga cagcctgcag gaactgttgc gcgccacggc cctccttctc tacatcccat
     3481 cgggaaagaa cacagaatcc gtgctaacgg gaaaaatctt cgattatctc aggacgggca
     3541 aaccggtgct tgccatcgtt ccgccggcag gtttggcagc ggaactgctc accaaagccg
     3601 gaattggcta cattgccgac catcaggacc ttcccggcat cgcggaaagg ctgatggaac
     3661 tctattggct ctgggaaaac cgcgcccttg gagaacttaa agtgaaccat gattacataa
     3721 cacagttttc ccgtccaaac ctagcccgca agctggctaa actcatagat gcagtggcaa
     3781 caggacagcc tgccggtttg ccagtccaca caggtgaaga acagtgaaag tcctttttat
     3841 accctcctgg ctgccttacc gggatgaccc cctggccggc aagttctttc tggatcaggc
     3901 tttggccatc gcggacgcag aggtggccga gatttttctt ttgaactggg gccaaaacga
     3961 gtttcaactg gctgtcaggc gtcccctctc cagcctgaaa aaattggcac ggcttattcc
     4021 taagcgccgc ggactgagcc agatagcgcc ccgactcacg gaaatccgcg ttccccatct
     4081 aagctggacc agtttggtgg caaaaggcaa catcgactcg ctgattccca agatcaagct
     4141 gccggaaaaa ccggacctca tccacgccca agtcactttc cccgcgggat atgtggccat
     4201 gcggctggcg gaaaagcacg gtcttcccta cattataaca gagcacagcg ggcctttccc
     4261 ctttcccgaa ttccttaaac gcggcgccat ttcgcccctt atcactgaac cgctgaaagc
     4321 agctgaaaga attgtggccg tcagcagcca tctaaaacag gaaatcagaa ccaaaacggg
     4381 gctggaagct ctggtgatcc ccaatccggt caacacggat tttttcattc catcggcaaa
     4441 tcctcgtcc
//
LOCUS       DAOOWR010000124         4437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19723_length_4437_cov_3.504336, whole genome shotgun sequence.
ACCESSION   DAOOWR010000124 DAOOWR010000000
VERSION     DAOOWR010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19723_length_4437_cov_3.504336"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PLB85_04080"
     CDS             complement(<1..1118)
                     /locus_tag="PLB85_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOU72539.1"
                     /translation="MPKGSVFHVNLGEDLPPDPIFEPQYRRAPHRGMPLSAHETKLAL
                     MNALRYIPTHLHSRLAPEFLQELKDHGRIYGYRYRPAGKIWGRPIDEYKGTVAGRAMQ
                     VMIDNNLDFDVALYPYELVTYGETGQVCQNWMQYRLIKKYLEVITDEQTLVVQSGHPL
                     GLFPSRKEAPRVISTNGLVIGKWDNPEEFQRLTALGVANYGQMTAGGWMYIGPQGIVH
                     GTFITLLNAGRKYLGIPADRDLAGVLYISSGLGGMSGAQSKAVEIAGGIGIIAEVDPS
                     RIETRYQQGWVSRVSKSPEEIFRWVEEFRTSRKPLSIAYHGNIVDLLEYVDKHEIKAE
                     LLSDQTSCHAVYEGGYTPAGYSFEEGRQLLANDPMKFRA"
     gene            1712..3766
                     /gene="fusA"
                     /locus_tag="PLB85_04085"
     CDS             1712..3766
                     /gene="fusA"
                     /locus_tag="PLB85_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU72540.1"
                     /translation="MKEYKLSKLRNLLLMGASGAGKTTLAEQIFHLTHTTNRLGKIDE
                     GNTVMDFDAEEIAKKTSLSLSLGWLNYKDHKINILDTPGTPDFIGDAIVAIPAVENAV
                     LVANAASGFEVGLELAIEHLENRKVGKVVVVNRMDNEHADFHKTLESIHENAGFNPIP
                     VHLPIGRENTFEGVVDIIRQKAIRPSGEGEVPADMQSAVEDARLHLMEAVAETDEELL
                     NAFLENMELSEEQLLKGLKNAVARGEVCPAFACSANTGVGVLALLDAVCSYLPSPEEA
                     NPMQVLMKGEPAEFIAKADGELLGYVFKLYADPNMGDYAYVRMFSGSLKSGMEFYIPE
                     KDAKDKAGNMYYMLGKNRSDTQEIRAGEIGALVKLKNAKALNSIVAVGSDLSLPVPEL
                     PTPTYWQMIRAANQSDEDKISTSLQRVIAEDPTIGYELNPETHENVISGMGEQQLQLV
                     LKKLKNRYKVEAIMKEPRIPYKETITASAESQYRHKKQSGGRGQYGEVYFRIKPTERG
                     EGFEFINAIVGGVIPSNFIPAIEKGLVETMEKGIVAGYQVVDLSVEVYYGSYHDVDSS
                     EMAFKIASSMALKEGFKKCRPILLEPIHDLTVIVPSEYMGDVMGDISTRRGRIMGMEQ
                     RGKKQYLNAQMPVAEMYFYYPALKSFTQGRGRFTQKFSHYEKVPDEIAAKVIAAWQDS
                     EA"
     gene            3915..>4437
                     /locus_tag="PLB85_04090"
     CDS             3915..>4437
                     /locus_tag="PLB85_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU72541.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQLFTRIVKEIILAAKNGGGDAE
                     MNPRLRTAVLTAKAANMPRENIERAIKRGTGEIEGAAYEEIVYEGYGHNGVGIVVEVM
                     TDNKNRTVADVRHIFSKYGGNLAESGSVAWNFEQKGFFNVPSAGLDEDEFMMQALEAG
                     AEDIELSDEYFDIY"
BASE COUNT         1101 a         1194 c         1210 g          932 t
ORIGIN      
        1 gcacggaact tcatggggtc atttgccagc agttgccggc cttcttcaaa gctgtatcca
       61 gccggggtgt agccaccttc atacacggcg tggcaggagg tttggtcgga gagcagttca
      121 gccttgatct catgcttgtc cacgtattcc aaaaggtcca cgatgttgcc gtgataggca
      181 atggaaaggg gtttgcggct ggtccggaac tcctccaccc agcggaagat ttcctcggga
      241 gatttggaca cgcggctcac ccagccctgt tggtaccggg tttcaatgcg gctggggtca
      301 acttcggcga tgatgccgat gccgccggcg atttccaccg ctttggattg cgcaccgctc
      361 atgccgccca aaccggatga gatgtaaaga accccggcca ggtcacggtc ggcggggatg
      421 cccagatatt tgcggccggc gtttaaaagc gtgatgaagg tcccatgcac gattccctgc
      481 ggcccgatgt acatccatcc tccggccgtc atctgcccgt aattggcaac gcccaaagcg
      541 gttagacgct ggaattcctc cggattgtcc catttgccga tcaccaggcc gttggtggaa
      601 atcacccgcg gagcctcctt gcgcgaggga aaaagcccca ggggatggcc ggactgcacc
      661 accagcgtct gttcgtcggt gataacttcc aaatatttct tgatcagtct atactgcatc
      721 cagttctggc aaacttggcc agtctcgcca taagtaacca gttcataggg atagagggca
      781 acatcaaaat ccaggttgtt gtcgatcatc acctgcatgg cgcggcctgc cacggtgcct
      841 ttgtattcat ctatggggcg gccccaaatc ttgccggcag gacggtagcg gtagccgtaa
      901 atcctgccgt gatccttcaa ttcctgcaaa aactccgggg ccagccggga atgcaggtgc
      961 gttggaatgt agcgcagcgc gttcatcagc gccagttttg tctcatgcgc gctcagaggc
     1021 atgccgcgat gcggcgcgcg tcggtattgg ggttcaaaaa tggggtccgg aggcaaatcc
     1081 tcgcccagat tcacgtgaaa aacactgcct tttggcatcg taaacctcat cattataata
     1141 tggtaagcgc ccggattggg atttctggcc atcacctgac tgcaggcgat acctgctcaa
     1201 attgaagcgc tttttttatg ttagctaata atgtccataa gcttgttttt gtcaagctaa
     1261 aagtctctcc agcctgttca ggatcgggac ggggcactgg tgaaagccgc ttatcctatg
     1321 cttttactga gtaatgtatc ggctgttaat catcaacatt actatatgga tagctcatat
     1381 tcaaattgag cgggttaaac gattataaac gttagccctg acaatatgag gaagcttttc
     1441 cgtctgtttg gccctttgcc ccttcccatt cgcctccttt tcgcctctcg ctgacttccc
     1501 gtttggaagg cgggaactct acgggaagtg aatgtgatgc agaagggata gggcaaagga
     1561 ggaatcacgg ctgtgacagg ggaaaaccta ttggggaaaa aagcgtttga caactatggg
     1621 caccggaaaa tactgactta ttcatggcgg gaattagtcc caagaatagc taatacgctt
     1681 ttatataaaa agatagagaa atgaggacca aatgaaagaa tacaaacttt ccaaactgcg
     1741 caaccttctt ttgatggggg cgagcggcgc gggcaagacc accctcgcgg agcagatttt
     1801 ccatttgacc cacaccacca accgcctcgg caaaatcgac gaaggcaaca ccgtgatgga
     1861 ttttgacgcg gaagagatcg cgaaaaagac atccctgagc ctgagcctgg gctggttgaa
     1921 ctacaaagac cacaagatca acattctgga cactccaggc accccggatt tcatagggga
     1981 cgcgatcgtt gccatcccgg ccgttgagaa cgctgtgctg gtggcaaacg cggccagcgg
     2041 ttttgaagtt ggcctggaac ttgccatcga gcatctggag aaccgcaagg tcggcaaggt
     2101 cgtggtggtg aaccgcatgg acaacgagca tgccgatttc cacaagaccc tggaatccat
     2161 ccacgaaaac gccggattca acccgattcc ggtgcatctc cccatcggca gggaaaacac
     2221 ctttgaaggc gtggtggaca tcatccgcca gaaagccatc cgcccttccg gagagggtga
     2281 agttcccgcc gacatgcaaa gcgcggttga agatgcccgc ctgcatctga tggaagcggt
     2341 ggcggaaacc gacgaagaac tgctcaacgc ttttctggaa aacatggaac tcagcgaaga
     2401 gcaactgctg aaaggcctta aaaacgccgt ggctagaggc gaggtctgcc cggcatttgc
     2461 ctgttccgcg aataccggag tgggcgttct tgctttgctg gacgcggttt gcagctacct
     2521 gccctcaccg gaggaagcca atcccatgca ggtgctgatg aaaggcgaac cggctgaatt
     2581 catcgccaaa gccgacggcg agcttttggg ttatgtgttc aaactttacg ccgacccgaa
     2641 catgggtgac tatgcctatg tgcgtatgtt ttcaggcagt ttgaagtccg gcatggagtt
     2701 ttacatcccc gaaaaagatg ccaaggacaa agccggcaat atgtattaca tgctgggcaa
     2761 aaaccgcagc gacacccagg aaatccgggc cggtgagatc ggcgctctgg tgaagctgaa
     2821 aaacgccaag gcgctgaaca gcatcgtggc tgtgggcagc gacctgagcc tgcccgttcc
     2881 ggaacttccc accccaacct actggcagat gatccgcgcc gccaaccaga gcgacgaaga
     2941 caagatcagc accagcctgc agcgcgttat tgcggaagat ccgacaatcg gttacgaact
     3001 gaatccggaa acccatgaga acgtgatttc cggcatgggt gagcaacaac tccagttggt
     3061 tctcaagaaa ctgaaaaacc gctacaaagt cgaagccata atgaaggaac cccgcattcc
     3121 gtataaagag accattaccg ccagcgcgga atcgcaatac cgccacaaaa agcagtccgg
     3181 tggccgcggg cagtatggcg aagtctattt ccgcatcaaa cccacagagc gcggcgaggg
     3241 cttcgagttc ataaacgcca tcgtgggcgg cgtgatcccc tctaacttca tccccgccat
     3301 cgaaaaaggc ttggttgaaa caatggagaa gggcatcgtg gccggctatc aggtcgttga
     3361 tttgagtgtg gaggtctatt atggcagcta tcacgatgtg gacagctctg aaatggcttt
     3421 caagatcgcc tcgtccatgg cgctgaagga aggcttcaaa aaatgccgcc ccatcctgct
     3481 ggagcccata catgacctaa ccgtcatcgt tcccagcgaa tacatgggcg atgtgatggg
     3541 cgatatcagc acccggcgtg gacgcatcat gggaatggag cagcgcggca agaagcagta
     3601 tctgaacgcc cagatgcccg ttgccgagat gtatttctat taccccgccc tcaagtcctt
     3661 cacccagggc cgcgggcgat tcacgcagaa attctcccac tacgagaagg ttcctgacga
     3721 gatcgcggcc aaagtgatcg ccgcctggca ggattccgaa gcataaactt actgcttttc
     3781 caagcagttt cagattccgg ggaaggttgg tcacacagtc ttcccctttt tacgcttgac
     3841 attttttcgc cccggccaga gactggtttg gattgaataa ataacgtaat tcaataagac
     3901 acaaagaggt ttgtatgtcc ggacacaata aatggagttc gatcaaacac aaaaaaggcg
     3961 cggccgacgc caaacgcggc caactgttca cccgcatcgt taaagagatc atcctggccg
     4021 ccaaaaacgg cggcggcgat gccgaaatga atccccgcct gcgcacagcc gtcctcaccg
     4081 ccaaagcggc caatatgccc cgggaaaaca ttgagcgtgc catcaagcgc ggcaccggcg
     4141 aaatcgaggg cgctgcctac gaagagatcg tctatgaagg ctacggccac aacggcgtgg
     4201 gcatcgtggt ggaggtgatg acagacaaca agaaccgcac tgtggctgat gtgcgccaca
     4261 tcttttccaa atacgggggc aacctggcag aaagcggctc cgtggcttgg aacttcgagc
     4321 aaaaagggtt tttcaacgtt ccttctgccg gcctggatga agacgaattc atgatgcagg
     4381 ccctggaagc cggtgcagag gacatcgagc tcagcgacga gtatttcgac atttaca
//
LOCUS       DAOOWR010000125         4428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_19769_length_4428_cov_4.273039, whole genome shotgun sequence.
ACCESSION   DAOOWR010000125 DAOOWR010000000
VERSION     DAOOWR010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19769_length_4428_cov_4.273039"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLB85_04095"
     CDS             complement(<1..304)
                     /locus_tag="PLB85_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72542.1"
                     /translation="MKRLILIFSILCLAWNVWAAADLYSGGEARPHYANDLVKVKLSL
                     AAAQRSNLPQQLMEETSRFGLADLDQILTETGGTAVIRAHRRLKNKAWEAAQGFDRW"
     gene            complement(781..2685)
                     /gene="thrS"
                     /locus_tag="PLB85_04100"
     CDS             complement(781..2685)
                     /gene="thrS"
                     /locus_tag="PLB85_04100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOU72543.1"
                     /translation="MPIKVTLPDGSARLYEQPVSALKVAEDISPRLAEAAICAELDGV
                     LVDLGHDIDRDAKLVLHTFKTDAGKDIYWHSTAHLMAQAVKQLFPDVKVTIGPSIEQG
                     FYYDFDRDKPFTEEELEQIEERMVSLSKQNLPYTRRELSKAEAMEIFSAMGEDYKLEI
                     LNEIPDGDIISTYTQSDFIDLCRGPHIPHTGKIKAVKLLKSSGAYWRGDEKNKMLQRI
                     YGISFPTKKELEEYLDFLKQAALRDHRKLGKDLDLFSINEDIGPGLVLWHPNGAMIRH
                     LIETYWKEEHLRHGYKLVYSPHVGRSNLWETSGHLGFYKENMYAAMDVEGQDYYIKPM
                     NCPFHIAIYNSNLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTP
                     DQLNSEVEKLIVFSLDMLKHFGFREFLIYLSTKPEESVGDPADWDTATASLRSSLEKI
                     GLEYQVDEGGGAFYGPKIDIKIKDALGRAWQCTTIQFDFNEPVRFDMSYIGADNAQHR
                     PYMVHRAILGSVERFFATLLEYHSGNLPHWLAPVQMMILPITEAQMDYARKLEEDFRL
                     QGLRCEVDTRNEKIGYKIRDAELKKIPFMCVIGKKEEESGQITLRQHTVGDLGSMSFA
                     DALSRIRSES"
     gene            complement(2749..>4428)
                     /locus_tag="PLB85_04105"
     CDS             complement(2749..>4428)
                     /locus_tag="PLB85_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOU72544.1"
                     /translation="SKQLFSEQLLMSIATIGAVILGEYIEAAAVMVLYEFGQWLESRA
                     LEHSRRSIRGMLSLKPDKTHLKTTAGVVETRLGEVLVGATIIVNPGERVPLDGVILKG
                     VSTVDTSTLTGEAEPLYVNPGAEIFAGFLNHSGRLEIKVTSSEAESTVTRILNLIENA
                     GTRKSQQEKFITRFARIYTPVVVGIAILVFLIPTILGHPAAVWFRRSLVFLIVSCPCA
                     LVISIPLSYYIGIGVAAKKGIIFKGSEYLDVLRRVKTVVFDKTGTLTTGEMKVEKVFV
                     REGSEPVELMHSLWLAEHSSSHPFAKAIRSAYEGDFDPALVNAYSEYPGRGVLLQYGQ
                     DRLLAGSEAFLKEFGFIDPIPAKSHSAVHAAKNDFYLGCVTFSDTLKPGIKDTLAQLK
                     TQGVERAIMLSGDRREKADKVSRETGLDEFHAELLPSHKLERLEEIMAREQGKTAFVG
                     DGMNDAPALARADAGIAMGAIGNQASVETADIVLLNDKPEQLAAAFSISRRTGMLVAQ
                     NIAIALGVKALVMVLGVGGISGLWEAIIADVGVTLIVIFNSLRLLRSERRA"
BASE COUNT          913 a         1162 c         1296 g         1057 t
ORIGIN      
        1 accagcggtc aaagccctga gcggcttccc aggctttgtt tttcaagcgc cggtgggccc
       61 ggatcacggc tgttccaccg gtttcagtca ggatttggtc aaggtcggcc aggccgaagc
      121 gggaagtttc ctccatcaac tgctgtggca ggttggaccg ctgtgccgcg gccaggctga
      181 gtttcacctt cacgaggtca ttggcatagt ggggacgcgc ttcgccgcca gaatatagat
      241 cagccgccgc ccagacattc caggcgaggc agagtatgct gaagatcagg atcaatcgtt
      301 tcaccgcgct gctccaatcg ttttttgcgt gataaagtgc aagattcatt ccctaaatgg
      361 gattatctgt cggttggctt atttgagtaa gaattattgg tccatgtagt gatgataaag
      421 ccttccaagc cgctttccgg ttgagcgttc gcaaccactt ggtttgaaaa atagccaaaa
      481 atacttgcca actgccatct cccgtctgcc agacccttag cggttgagtc catccctctc
      541 ccatacctcc cgcacaattc tcgcattcaa tgcgggtatc atacgggagg cgcgtcaggt
      601 tcactggaca gggccttaac ggccaacctg catcgcttcg ctcatttgca agaatttctc
      661 gatgcaagca tatctgacca tccctcagta aatcaaggct tccgatgcca gtgccaaaaa
      721 agccccgaag ccatttgact tcggggcctt atatcatctt atgacagtct tcggaaaggg
      781 tcaggactcg cttcgtatgc ggctgagggc atcggcgaag ctcatggaac ctaggtcgcc
      841 aacggtatgc tggcgcaggg tgatctgtcc ggattcctct tcctttttac cgatcacaca
      901 catgaagggg attttcttga gttccgcgtc gcggattttg tagccgatct tttcgttgcg
      961 ggtgtcaact tcgcagcgca ggccctggag gcggaaatct tcttcgagct tgcgggcgta
     1021 gtccatttga gcttcggtga tgggcaggat catcatttgc acgggagcca gccagtgggg
     1081 caggtttccg gagtggtatt ccagcagggt ggcgaaaaag cgttccacag agccaaggat
     1141 ggcgcggtgc accatataag gccggtgctg ggcattgtcg gcgccgatgt agctcatgtc
     1201 gaagcggacg ggttcgttga aatcgaactg gatggtggtg cactgccagg cgcggccgag
     1261 ggcgtctttg atcttgatat cgatcttggg accgtaaaaa gcgccgccgc cttcatccac
     1321 ctgatattca aggcctatct tttccagcga ggagcgcagg ctggcggtgg ctgtatccca
     1381 atcggcggga tcgcccacag actcttccgg cttggtgctc aggtagatga ggaattcgcg
     1441 gaaaccgaag tgcttcagca tgtccaggga aaagacgatg agtttctcca cctcggaatt
     1501 gagctggtcc ggggtgcaga tgatgtgggc atcgtcttgg gtgaagcctc taacccgcat
     1561 caggccatgc aaaacaccgg agcgttcata acggtaaacg gtgcccagtt ccgctaagcg
     1621 gatgggaagt tcgcggtagc tgtgcaggtt tgaattgtag atggcgatgt ggaaggggca
     1681 gttcatcggt ttgatgtagt aatcctggcc ttccacgtcc atggccgcgt acatgttttc
     1741 cttgtaaaaa cccaggtggc cgctggtctc ccaaaggttg gaacggccca cgtggggaga
     1801 gtaaacaagc ttatagccgt ggcgcagatg ctcttccttc caatatgttt cgatcaggtg
     1861 acggatcatg gcaccgtttg gatgccagag aacgagaccg gggccgatgt cttcgttgat
     1921 ggaaaagagg tcgaggtcct tgcccagttt gcggtggtcg cgcagggcgg cctgttttag
     1981 aaaatccaga tactcttcca attccttctt agtggggaag ctgatgccgt agatgcgttg
     2041 gagcatcttg ttcttttcat ctccgcgcca gtaggcgccg gaggatttga gcagcttcac
     2101 ggccttgatc ttgccggtgt gggggatatg cggtccgcgg cagaggtcga tgaaatcgct
     2161 ttgggtgtag gtgctgatga tgtcgccgtc aggaatttcg ttcaggattt ccagtttgta
     2221 atcctctccc atggcactga aaatctccat cgcctctgct ttggatagtt ccctgcgggt
     2281 gtagggcaga ttctgtttgg agagggagac catgcgctct tcgatttgct ccagttcctc
     2341 ttcggtaaag ggtttgtcgc ggtcgaaatc gtaataaaaa ccctgctcga tggacgggcc
     2401 gatggtcacc ttcacgtctg ggaagagctg tttcacagct tgtgccatca gatgcgcagt
     2461 gctatgccag tagatgtctt ttccggcgtc tgtcttgaag gtgtgcagca ccagtttggc
     2521 atctcggtca atgtcgtgcc ccaagtccac caaaacgccg tcgagttcgg cgcagatggc
     2581 ggcttctgcc agcctggggc tgatgtcttc agcaaccttg agggcgctta caggctgctc
     2641 gtaaagcctg gcggagccgt cgggcaaagt aacttttatc ggcatggtat ctccaacaat
     2701 tatagaatta agttcaacaa ggcaaggcag tttaggcggg gatttttgtc aagcgcggcg
     2761 ttcggaacgc agcagcctca gcgagttgaa gatcacgatc agggtgacgc ccacatcagc
     2821 gatgatggct tcccaaagcc cggatatgcc gcccacgcca aggaccataa ccaaggcttt
     2881 cacacccaga gcgatagcga tgttttgtgc caccagcatt cccgtgcggc gggaaatgct
     2941 gaaggcggca gcaagctgtt ctggtttgtc gttcagcaac acgatgtcgg ctgtttccac
     3001 ggaagcctgg ttgcctattg cgcccatggc gattcctgca tccgcgcggg ccagggccgg
     3061 agcgtcattc atgccgtctc ccacaaaggc ggttttgccc tgttcgcggg ccatgatctc
     3121 ttccagacgc tcaagtttat gagacggcag cagttccgcg tggaattcgt ccagcccggt
     3181 ctcgcggctc accttatccg ctttttcacg gcggtcgccg gagagcataa tggctcgttc
     3241 cacaccttgc gttttcaact gtgccagggt gtctttgatg ccgggtttga gggtgtcgga
     3301 aaacgtcacg caacccagat agaaatcgtt cttcgcggca tggacggcgc tgtgtgactt
     3361 agccgggatg gggtctatga agccgaattc cttcaaaaag gcctccgaac ctgccagcag
     3421 gcgatcctgg ccatattgga gcagaactcc gcggccgggg tattcagaat aagcattcac
     3481 caatgcagga tcgaaatcac cttcgtaggc gctgcggatg gctttggcaa agggatgaga
     3541 gctgctgtgt tccgccagcc aaagactgtg catgagttct acgggctcgc tgccttcgcg
     3601 gacaaaaacc ttttccacct tcatctcgcc agtggtgagg gttccggttt tgtcaaacac
     3661 cactgttttc acccggcgca acacgtccag atactcgctg cccttgaaga tgatgccctt
     3721 tttggccgcc acgccgatgc cgatgtaata ggaaagcggg atggaaatca ccagcgcgca
     3781 ggggcaggaa acgatgagga aaaccaggct gcgccggaac cagacggcag ccggatgtcc
     3841 tagtattgtg gggatgagga agaccaggat ggcgatgccg accacgaccg gggtatagat
     3901 ccgggcgaag cgggtgatga atttttcctg ctgcgatttg cgtgtgccgg cgttttcgat
     3961 caggttcagg atgcgggtta cggtgctttc cgcctcgctg ctggttacct tgatctccag
     4021 tcggccgctg tggttcagga atccggcgaa gatttccgcg ccggggttca cataaagcgg
     4081 ctctgcctcg ccggtgagag tggaagtgtc aaccgtgctt acgcccttca ggatcacgcc
     4141 gtcgagaggg acccgttcgc caggattgac gatgatggtt gcgcccacca gcacttctcc
     4201 caggcgggtt tccaccactc cggcagtggt tttgaggtgg gtcttatccg gtttgagtga
     4261 cagcatgccg cggatcgagc gacgtgaatg ctccaacgcg cgggattcca accattggcc
     4321 aaattcgtag agaaccatca ccgcggctgc ttcaatatat tcacccaaaa tgacagcacc
     4381 aattgtagct atgctcatca gtagctgttc ggagaaaagc tgcttgga
//
LOCUS       DAOOWR010000126         4385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_20010_length_4385_cov_3.653118, whole genome shotgun sequence.
ACCESSION   DAOOWR010000126 DAOOWR010000000
VERSION     DAOOWR010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20010_length_4385_cov_3.653118"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLB85_04110"
     CDS             complement(<1..1106)
                     /locus_tag="PLB85_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72545.1"
                     /translation="MNKLMRSFLGLALLAVLVSCGRSGPTAPQPTAGTLVFSEDKLPC
                     DGDEYIYRQGISAGPAVPENALFAWRVETLSGELPQGWFADPEGWLWFRAPGADLEVS
                     LAEEGPHRSIWTTRGSLSFDFASSEGKISNLVKKVDLRVKSKDSQINTYSSGFKSDRL
                     IGSLINTAIEPGANTGTGIEFALREVIGDIYVDGLYADHFMFRLNILNKDLEVISEGV
                     WHSSLEMADLRKVRLNATTDPALGENAHNQYTQFESYVVSRQGIEEATPQSVYFRVRG
                     NFKPKALIYTQALAALGEHHYSVNPLEQLYYKELIPPAALHNNRSLWETDAGWEAINS
                     PDLKLHLQWGYLGQYGSTNPPWSGMEGFIPGGPF"
     gene            complement(1594..2196)
                     /locus_tag="PLB85_04115"
     CDS             complement(1594..2196)
                     /locus_tag="PLB85_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72546.1"
                     /translation="MSPKAFLLLLLLLPGLLLAQPQFAFEGEVLDFSLEKTSPESDTL
                     LWIVSGRFYLSNLHHEALSRLIWFPVPASDSVGVAEDVSISLIEPVDSMSVELLYQNQ
                     RGFGFRLDLPARSFAGVKISYRQKISGSEARYVLLTANSWGRPLPSSEINLTLGQGIT
                     LTDLPYPDPIASLDAQNSNYRWTFLDFVPDRDFVVRFSCL"
     gene            complement(2193..3245)
                     /locus_tag="PLB85_04120"
     CDS             complement(2193..3245)
                     /locus_tag="PLB85_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72547.1"
                     /translation="MKKLLMLMAVMLFATALTANPITPRNLARLWFDSGDLFLQFAED
                     HGYYGYLDRIPELTFVTSAGTWQFPDSFVPPTSLPFTVNISEAIPGFSIDPLNDQLVL
                     QDSISWNSQWQESVSWGLGDAVDLRSLQPGQSAVQVMVGTYEGEVAAWAKDIGTEGGF
                     NPVHCYTLSVHTQNHQGIPVSGVPVYLSFTGYISTYYTPYTTDADGNWQLASSAVPTR
                     VRVLDPLTQEIVLDTRFYPDPEETVSLTAVISSAAADDPVQPSASHVLSISPSVLNSA
                     SGNTVSLKYGQGISLSESAELKLYDLRGRVLASTGMPSVAEIQWRLPELPSGIYFIAL
                     EQGSRQLGRGRISVVK"
     gene            complement(3253..4323)
                     /locus_tag="PLB85_04125"
     CDS             complement(3253..4323)
                     /locus_tag="PLB85_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72548.1"
                     /translation="MDVKKMVSAAALLLVISALAANIQIPRAVERVWFDDSDNFWVRF
                     GPEADAWDVGSLGFATSSGTYNFPCDYTLPLELPFEINLSQAIPGFTINRAEDFFTVD
                     TDPYDDPLDETVSWGPQNDTSVNIHPLTAGQSVVQYSVGYQIYMGEWESMHVWAKDNG
                     TAYVHPYCPATLCTLSVQVNDLSGQPASSVPIHLNYGTYPDTVWPDAHTNSNGWWQKQ
                     YFYAQRIRVIIKDPVTGTWVYNQLHFPEPGEHIHIEAIVTSSGVVPEELMPKTLELSP
                     SVLGTSSGNTLHVEYRSKQPLERAARLDLYDLRGRWMAGREMPREGYTDWVLPDLANG
                     IYFIRLSGSRGPLVGGRITIIK"
BASE COUNT          985 a         1148 c         1269 g          983 t
ORIGIN      
        1 tcgaagggac cgccggggat gaagccttcc atgccagacc agggcggatt ggttgaacca
       61 tactggccca gatagcccca ctgcagatgc agcttcaggt ctggggagtt gatcgcctcc
      121 cagcctgcat ccgtttccca gaggcttctg ttattgtgca gggcggcagg cgggatcagc
      181 tccttgtaat agagttgttc caacgggttg acactgtagt gatgctcccc cagcgcggcg
      241 agggcttggg tataaatcag ggctttgggt ttgaaattgc cccgcacccg gaaatagaca
      301 ctctggggag tggcctcctc aatgccttgg cggctaacca catagctttc gaactgggtg
      361 tattgattat gggcgttttc gccgagcgca ggatcagtgg tggcgttcag cctcactttg
      421 cgcaaatccg ccatctccag cgaactgtgc cagacgcctt ctgaaatcac ttccaggtct
      481 ttattcagta tgtttagccg aaacatgaag tggtccgcgt aaaggccgtc cacataaatg
      541 tcgccgatca cttcacgcaa agcgaattcg attccagtgc cggtgttggc gccgggttcg
      601 atggcggtgt tgatgaggct gccgatcaag cggtcgctct tgaagccgga gctgtatgta
      661 tttatttgag agtcttttga cttgaccctt aaatccactt ttttaacgag gttgctgatt
      721 ttgccctcgc tggaagcaaa gtcgaagctg aggctgccgc gtgtggtcca aatgcttcgg
      781 tgcggccctt cctccgccag ggaaacctcc aaatccgctc cgggcgcgcg aaaccacaac
      841 caaccctcgg gatcggcaaa ccaaccttgt gggagttcac cgcttaaagt ctctacccgc
      901 caggcgaaga gggcgttctc cggcactgcg ggtcccgcgg aaatgccctg ccggtagatg
      961 tattcatccc catcacaggg cagcttgtcc tctgaaaaaa ccagtgtccc ggcagttggc
     1021 tggggtgctg ttggcccgct cctgccgcag gaaacgagta cagccagcaa agccagaccc
     1081 aaaaaagatc tcattagttt gttcattact cactcccgct atcttttctt gtcttccatt
     1141 cgcggttgtc attctaactt tatcaaagta cttgtcaagc attattttgg gctctctttc
     1201 aaattggatg gataaagctc tctggactgc tttctgattg aatgcccgca accacttgat
     1261 ttaatagaga gccataaatg ccaacttacc gcaatgtcct gtcggtcagg ccctgagcgc
     1321 tcgagaccgt ccctctcttt tgtcccccgc ccaatactcg catcaaatgc gggcatcatg
     1381 cgggaggcgc gacagagtta cataaaaggg ccttaacagc cagtcaggat cgcttcttcc
     1441 tggccgcttc aaacacctgt tatcgtatgc cataccaagg cgttacccgc ccgggtttaa
     1501 tgaacatcat ggatttcagg gatggaaagg atgtttggtc gggaagttca gattctggat
     1561 ttgtttctga ttcacctgcc ggataggatc aattcatagg caactgaaac gaaccacgaa
     1621 atcacggtcc ggaacgaaat ccaaaaacgt ccagcggtag ttggagtttt gagcgtcgag
     1681 gctggcgatg gggtccgggt aaggcagatc ggtgagcgta attccttgtc cgagagtaag
     1741 gtttatctcc gaagaaggca gcggccggcc ccaggagtta gcggttagca gcacatagcg
     1801 ggcttccgag ccgctaatct tttgccggta gctgattttc actcccgcaa agctgcgcgc
     1861 gggcaaatcc agcctgaagc caaagccgcg ctggttctgg tatagcagtt ccaccgacat
     1921 gctgtccacg ggttcgatga ggctgatgga tacatcctcc gcgaccccga cgctgtcgga
     1981 agcgggaacg ggaaaccaga tcaggcggct tagtgcttca tggtggagat tggaaaggta
     2041 gaaccggcca gagacaatcc agagcagagt gtcgctttcc ggggaggttt tctccagaga
     2101 aaaatccagc acctcgcctt caaaggcgaa ctgcggctgg gccagcagca gccccggcaa
     2161 cagcaagagc agcagcaaaa aggccttggg gctcatttga cgacgctgat ccggcctcgt
     2221 cccaactggc gggaaccctg ctccaaagca ataaaataga tgccgctggg cagctccggc
     2281 aagcgccatt gaatctcagc tacagacggc atcccagtac ttgccaaaac acggccgcgc
     2341 aggtcgtaaa gcttaagttc ggcagattct gagagggaga ttccctggcc gtatttaagc
     2401 gagacggtgt tcccgctggc agagttcagg actgaagggc tgatacttaa tacatggctg
     2461 gcagagggct ggacaggatc gtctgcggct gcgctgctga tcacagcggt gagcgaaacg
     2521 gtttcctccg gatctgggta aaacctggta tcaagcacta tttcctgggt gagcggatcc
     2581 agaacccgta cccgggtggg tactgcggaa gaggctagct gccagtttcc atcggcatcg
     2641 gtggtgtatg gtgtgtaata tgtggaaata tagccggtaa aactcaaata gactggcact
     2701 ccagacaccg gtattccttg atggttctgt gtgtgcacgc tgagagtgta gcagtgaacg
     2761 gggttgaatc cgccctcggt gcctatgtcc ttggcccagg cggcaacctc gccttcatag
     2821 gtgcctacca tcacctgcac agcggattgg ccaggctgca gggaacgcag gtccacagcg
     2881 tctcccagcc cccagctgac cgattcttgc cactgcgaat tccacgagat gctgtcctgc
     2941 agcaccaatt ggtcattgag gggatcaata ctgaaaccgg gaatggcctc agatatgttc
     3001 acagtgaagg gcagggaagt gggtggaacg aaagagtctg ggaattgcca ggttccggct
     3061 gaggtgacaa aggtaagttc cggaatgcgg tccagatagc catagtagcc atggtcttcg
     3121 gcgaactgga gaaagaggtc gccggaatca aaccaaagcc gggccaggtt tcggggtgta
     3181 atgggattgg ccgtcaaagc tgtggcgaac agcattacgg ccataagcat aagcagcttt
     3241 ttcatggtta cctcacttta tgatggtgat acggcctcca accagagggc ctctggagcc
     3301 gctgagacgg atgaagtaaa tgccgttggc aaggtccggt aaaacccagt ctgtgtatcc
     3361 ctctctgggc atttccctgc cggccatcca tctgccgcgc aaatcgtaga ggtccagacg
     3421 cgcggctctt tccagcggct gttttgatct gtattccacg tgcagggtgt tgccggacga
     3481 ggttcccaaa acggaggggc tgagctccag agttttaggc attagttcct cgggcacaac
     3541 accactgctc gtgactatgg cttcgatgtg aatatgctcg ccgggctcag ggaagtgaag
     3601 ttggttgtaa acccaggttc cggtgacggg atctttgatg atgacgcgga tgcgctgagc
     3661 atagaaatac tgcttctgcc accagccgtt ggaattggtg tgcgcgtccg gccagacggt
     3721 gtcagggtag gtgccgtagt tcaggtggat gggcaccgag gaagcaggtt ggccgctgag
     3781 atcgttcacc tgtacgctca gggtgcagag ggtggcggga caatagggat gcacataagc
     3841 tgtgccgttg tctttagccc agacgtgcat tgattcccat tcccccatgt agatctggta
     3901 gccgaccgaa tactgcacca cagactggcc ggccgtgagc ggatggatgt tcacgctggt
     3961 atcgttctgg ggcccccagc tgacggtttc atccaaggga tcatcataag ggtccgtatc
     4021 cactgtgaaa aagtcttccg cgcggttgat ggtgaaaccg gggatggcct ggctgagatt
     4081 gatttcgaac ggcaattcca gcggcagggt gtagtcgcag gggaaattgt aagtgccgga
     4141 agaggttgcg aagccgaggc tgcccacgtc ccaagcgtct gcctcagggc cgaagcgcac
     4201 ccagaaattg tcagaatcgt caaaccagac gcgctccacc gcccggggga tttggatgtt
     4261 ggcagccaaa gcgctgataa cgagcagcag ggccgcagcg gagaccatct ttttcacgtc
     4321 catttctcct cccaaggaaa gagtaagtca ttgatcagta aaggcaaatc cttttcatgg
     4381 gaata
//
LOCUS       DAOOWR010000127         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_20048_length_4380_cov_3.737341, whole genome shotgun sequence.
ACCESSION   DAOOWR010000127 DAOOWR010000000
VERSION     DAOOWR010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20048_length_4380_cov_3.737341"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLB85_04130"
     CDS             <1..629
                     /locus_tag="PLB85_04130"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72549.1"
                     /translation="ELPPGWLNPGSWDFPPDQYYPYHGTKCAQVRAGAAGNLLATPKL
                     QLTSESTLEFMALSNAAPGSAGFRVKRSSDGTNWNEVAILPAISAANVWQRFELDLGS
                     LAGQSYHLGIEAYNAGSGSNPLVFLDHVVGPRPVGVYPSPEVSISRRDNGFRLSWESP
                     GGVTGYRVLAADDVDSFGEEALAELSASQTFFDVPTGNRKFFRVTAIY"
     gene            complement(1166..1468)
                     /locus_tag="PLB85_04135"
     CDS             complement(1166..1468)
                     /locus_tag="PLB85_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOU72550.1"
                     /translation="MLSKRDRVRLRQFGRRFGSRPKTCHCHSGDCISLADLEPGVEAL
                     ITCNNDLKTIERGLYHGKRVMVQRNESGEPNLVVAVGDARYVLDRRVARMIRVKVV"
     gene            complement(1458..3557)
                     /gene="feoB"
                     /locus_tag="PLB85_04140"
     CDS             complement(1458..3557)
                     /gene="feoB"
                     /locus_tag="PLB85_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOU72551.1"
                     /translation="MSQDNPTIALAGNPNVGKTTLFNALTGSHQTVGNWPGVTVERKE
                     GFFIHNGRRYEVIDLPGIYSLAAGSPDELVARNYLVQKKPNLVINIVDASNLERNLYL
                     TLQLMELKVPLLIVFSMLDIAEQNGVEIDFDHLKSHLDCPIQPLTLCRRFDPSQLFQD
                     IDACLAKAPVPISPRYDEVVEKHLHNLHNFMLQQRDDPAWPEAWRKLPLDWVALKLLE
                     RDPYFHAELPQAWFDPVDREIRGLEKHRNQAAATALADDRYGFIRGLVKDVVRHKTKP
                     GATFSDRLDRVALSSLWGLPVFFAVMYLVFLLAVQASEPLIGWIENGLNWLLVDQWGA
                     LLRSWNIPGWLSYLLSDALGGGIATIATFIPPIFFIFLCLSLLEDSGYMARAAFIADR
                     FMRRVGLPGKAFIPLLVGFGCTVPAIMATRTLESRRDRVFASLLTPFMSCGAKLPVYT
                     YLGMLFFPGRADLAVFGLYFFGVVMGLLFALALKKTLFKTQPGNFVMELLPYHLPTIN
                     AIGMHTWHRLKDFILRAGKTILLVTVAVNILQALTLPVKGGQASVLELAGKLLTPLMK
                     PMGIVAENWQATVALISGLFAKEAIVGTLQSLYPDPSVIPSAFGGSSSGIAFLIFVLL
                     YSPCAASLAALNKEHGWRWSLFSFFYLTLLAWIVATVAYQILAFNRLSWLWLGLCALL
                     VVAFGASLKLIGRKNAI"
     gene            complement(3561..4001)
                     /locus_tag="PLB85_04145"
     CDS             complement(3561..4001)
                     /locus_tag="PLB85_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU72552.1"
                     /translation="MDNQVTVFEKFLFRKGLKLTTPRREILDAVFSLHEHFDAQQLHA
                     MLKDQKRRISLATVYRTLPLLEEAGLIQHSLRSSSRDVFERIYGHPRHVHWICSCCGW
                     VQETPLDGLKRSLLKEASSLKFRLDEVNIQVNGLCWKCCENENQ"
BASE COUNT         1049 a         1139 c         1222 g          970 t
ORIGIN      
        1 atgagcttcc cccaggatgg ctcaatccgg gaagctggga tttcccacca gatcaatact
       61 atccctatca cggcaccaaa tgtgcccagg ttagagccgg agcggcgggg aacctgctgg
      121 ccacacccaa gctgcagttg acttcggaat caacattgga gttcatggcc ttgagcaatg
      181 ctgcaccggg cagcgcaggt tttagggtca aacgctcatc tgacggcaca aactggaacg
      241 aggtggctat tttacccgct atcagcgccg ccaatgtctg gcaacggttt gagttggacc
      301 taggttccct ggcggggcag agctatcatc tgggcattga ggcttataat gcaggaagcg
      361 gaagcaaccc cctggtcttt cttgaccatg ttgtggggcc ccgtcccgtg ggcgtgtatc
      421 catctccgga agtgagcatc agccgccggg ataacggttt tcggctgagt tgggaatctc
      481 cgggcggggt caccggctac cgggtcttgg ctgcagatga tgtggattcc tttggagagg
      541 aggcgctggc tgaactgagc gcatcccaaa catttttcga tgtgccaacg ggtaacagga
      601 agtttttccg ggtgacggca atctattgat ccaacagatt tgatggcggg tgctgcttta
      661 gctgattctc cagcgccggg agccttgagg tcttgttgtg tctttgaaac tggcaataat
      721 atagggctct ctttcagatc gagcgggtaa ttcggtttga aagagagcca aataatattc
      781 ccaaaccccc atttgcctga tctgctcctc cttttcctcc tttgccccat ccctttcaca
      841 tcccattcac ttcccgctga gttcccgcct ttcaaacggg aactaaacgg gaggcgagag
      901 agaggcgaaa aagataggga aaaggaagaa tcatccagct ggatttcaag agtaaagcat
      961 tggggattat gcgattggct tatgttcaga cagggaggga aagactgtag tcatcccagt
     1021 gcaggctgga tagtgtcgtt ctgctgtggt cgaagttccc gcatcgttcg actccagaaa
     1081 agcttaggcc cttttacggt attcctccgt ttttcctctg ttttttcctg cttagctctg
     1141 tgaaaaaaac ttgtggataa aagcctcaga ccactttaac cctgatcatc ctggccaccc
     1201 ggcggtccaa aacatagcgg gcgtcgccaa cagcgaccac gaggttgggc tctccggact
     1261 cgttgcgttg gaccatcacc cttttaccgt ggtagagtcc cctttcaatg gttttgaggt
     1321 cgttgttgca tgttatcagg gcctccacgc cgggttcaag gtctgccagg ctgatgcagt
     1381 cgccactatg gcagtggcag gtttttggcc ggcttccgaa tctacggccg aactggcgaa
     1441 ggcgcacgcg gtcacgttta gatagcattt tttcttccaa tgagtttgag gctggctcca
     1501 aaggccacaa ccagcaaggc gcagagcccc agccagagcc aggacagccg gttaaaggcc
     1561 agaatctggt aagcgacggt agccacgatc caagccagca gggttagata gaaaaaggag
     1621 aagaggctcc agcgccagcc atgttccttg tttagggccg ccagggaagc ggcgcagggc
     1681 gaatagagca gcacgaaaat cagaaaggcg attccggaag atgacccgcc aaaagcgctt
     1741 ggaatgacgg acggatcggg ataaaggctc tgcagagttc ccacaatcgc ctccttggcg
     1801 aacagcccgg aaatcagggc aaccgtggcc tgccagtttt ccgcgacaat ccccatcggc
     1861 ttcatcagtg gagtgagcaa cttgccagcc agttccagaa cactggcctg gcctcctttg
     1921 actggcagtg taagcgcctg cagtatattc acagccacag ttaccagaag gatggtcttg
     1981 ccggcgcgca ggatgaaatc cttcaggcgg tgccaggtgt gcattccgat ggcgtttata
     2041 gtaggcagat ggtaagggag cagctccatc acgaagttgc cgggttgggt cttgaacagc
     2101 gtttttttca gcgccagcgc gaacagcagg cccataacca ccccgaaaaa gtagagtccg
     2161 aaaacagcca ggtcagccct tccgggaaag aacagcatac cgagatatgt gtaaaccggc
     2221 agcttggcgc cacaactcat aaaaggcgta agcagcgagg cgaaaacgcg gtcccggcgg
     2281 ctttccaggg tgcgggtggc catgatagcc ggaactgtgc agccaaaacc gaccaaaagc
     2341 gggatgaagg cctttccggg cagacccacc cgacgcataa acctatcagc gataaaagcg
     2401 gcgcgggcca tgtagccgct gtcttcaagc agggacaagc acaaaaagat gaagaaaata
     2461 ggtgggatga acgtggcaat ggtagcgatc ccgcctccga gggcgtctga aagaaggtag
     2521 ctgagccagc cggggatgtt ccagctgcgc agtagcgcgc cccattgatc caccagaagc
     2581 cagtttagcc cgttttctat ccatccgatt aagggttcgc tggcctgcac ggccaacagg
     2641 aaaaccagat acatcaccgc gaaaaagacc ggcaggcccc aaagactgct cagcgccaca
     2701 cggtcgagtc tgtccgaaaa agtggcgccc ggcttggttt tgtggcgcac cacgtctttc
     2761 accaaaccgc ggataaagcc atagcggtcg tcagccaagg ccgtggcagc agcttggttg
     2821 cggtgttttt ccaagcctcg aatctcgcgg tccaccgggt cgaaccaagc ttggggcaat
     2881 tccgcgtgga aatagggatc gcgctccaac agcttcaggg ctacccagtc cagagggagt
     2941 tttctccagg cctccggcca ggcagggtca tccctttgct gcagcatgaa gttgtggagg
     3001 ttgtgaaggt gtttttccac cacctcgtcg taacgagggc taattgggac tggagctttg
     3061 gccaggcagg cgtcgatatc ctgaaaaagc tggctgggat cgaagcggcg acagagagtc
     3121 agtggctgga tggggcagtc cagatggctt ttcaggtggt caaaatcgat ttccacaccg
     3181 ttctgctccg caatgtcaag cattgagaac acgataagca acggcacttt cagttccatc
     3241 agctgcagcg tgagataaag gttgcgctcc agatttgagg catccacaat gttgatcacc
     3301 aagttcggtt tcttttgcac taggtaattt cgggccacca gctcatccgg agatccagcc
     3361 gccagtgaat agatgccggg taggtctatc acctcataac gccggccatt atgtatgaaa
     3421 aaaccttcct tgcgctcaac cgtcacgcca ggccagttac caacggtctg gtgcgagccg
     3481 gtgagggcat tgaaaagagt ggttttgccc acgttggggt ttcccgcaag ggcgatggtg
     3541 gggttatctt gactcatagc ttattgattc tcattctcac agcatttcca acagaggcca
     3601 ttcacttgga tgttcacttc gtccagacgg aatttcaggc tggaggcttc tttcagcagt
     3661 gatcttttca agccgtccag cggcgtttcc tgcacccagc cgcagcagga acaaatccag
     3721 tgaacatggc gggggtggcc gtagatgcgt tcaaacacgt cacgggaaga ggaacgcagg
     3781 gaatgctgga taaggcctgc ctcctccagc agaggcagag tgcggtaaac cgtggccagg
     3841 gaaatccggc gtttctgatc tttcagcatg gcgtgcaact gctgggcatc aaaatgctcg
     3901 tgcaggctga ataccgcgtc cagtatctcc cgacggggag ttgtgagctt cagccctttg
     3961 cggaacagaa acttctcaaa aacagtgact tggttgtcca tggcatattc ctttaatgag
     4021 aatcagtcgc atttgtttca agcactgaat tcctgtcaag gactattgca agtctttccg
     4081 agttgctatt gaaagtataa atacgaagtg caaaccctca ctgaaaactt caaatccaat
     4141 caccaaaact gcaaatgtcc aagtccaaca aaacagtgtg tgaatgtcat atattgcctg
     4201 ccagtaatgt gatagcatca attgtccgag tgatagcaat ttgaaattgt cagttagggt
     4261 ttacagcgga cgcagtctaa accgcgggga tcaataccgg ggctcatttt tccttgccgg
     4321 gattcatgct tacgatgtcg cagagggaaa gccggtcctt gctgccggaa aaggaactga
//
LOCUS       DAOOWR010000128         4341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_20280_length_4341_cov_4.327811, whole genome shotgun sequence.
ACCESSION   DAOOWR010000128 DAOOWR010000000
VERSION     DAOOWR010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20280_length_4341_cov_4.327811"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..695
                     /locus_tag="PLB85_04150"
     CDS             144..695
                     /locus_tag="PLB85_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72553.1"
                     /translation="MFEFIKSLPGLYLSWVKAWPLLSAMIQFAILGTLGEIISKWVIR
                     KSFRYPFTFGLTLWKMLVWALLGVAIKYAFQGFTGFVEHLEAHSYLPPLNTFGRAFAI
                     SAFMNLQFGPFMVIVHRLLDNLGERKQNWKGIHKSMLALLWFWIPAHTVTFLQTPDLR
                     IGLAALLSLALGLILGIFNWRQA"
     gene            755..2968
                     /locus_tag="PLB85_04155"
     CDS             755..2968
                     /locus_tag="PLB85_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOU72554.1"
                     /translation="MAWCAGIGIARFLDPPFRPLLGLGALLLAAAVVLKNARPVLALL
                     LCLVLGALRWNAANEPSALDVVFRAKRHIQQDAEFTVTKLLSREAGIYEIRLEELAGV
                     KLREPLSLFSEEELIPGQAYTALLEVLPGKQDPILDMFPARHRAYVRQSLTPVDKPPR
                     MFPVALWRVRLLKNLDDKLGADSEFAKAFLFSDTSAKGQYRDQLTRSGMIHLIVVSGL
                     HIWFIYGICMVLLNALFPKRVAELAFLVLIAFYAALNHWSPPVTRAILMIGLMILGRW
                     RSIPLGGAQLLALSLLIITAVSPEQLFNIGLQLSYLCIGVIILGLPKIVWISEQRLPS
                     DALRGRLNRFFDLLLMNLVVGLAVLPLTLHYLGTASLNGILGNLLGIPLSGALLTVSF
                     IVLLIPGGNWLCNIYIASYRLLLCAFNRWTEFVARLPLWLENTWISGLQLFGAMLLVV
                     SMLYLLRRLEFSWKVLPVAVLGLALLFLPQFRSKPEGGIYLFACGTGDCILASFPGGE
                     TLLVDTGPKFHNAEKSWAAGKLLPWLARKNICRIDWMVLTHLDSDHSGGFPDLAKALS
                     INNLAVTDETIQDPKWVEWSESGWLDGIRVHTISDTISYRMGEARLKFLHPDRDYFTE
                     SSNSASLVFRLDYLNTSYLFTGDADIDAEAHLLASYPEELKADFLKAGHHGSRSSSSQ
                     EFVRAVMPDEVWITVSDRNQWGFPHPEPLNAFRRYARRISSTSSGSIHQPFAQKD"
     gene            3027..>4341
                     /locus_tag="PLB85_04160"
     CDS             3027..>4341
                     /locus_tag="PLB85_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72555.1"
                     /translation="MPTPSARALGLPAVFALIELAGEFPAEVKKQELKRPPLKFADAK
                     HRYAKLEQVIALLRGEDGALRDLRELFARIPKLSLPQRGGKAVFALHELFLLKEHLYH
                     YGRLHAWLRRKGWLDTFILPDLGKVFALLDPDGTELPAFHLSAAYSAKLGEILSAQFE
                     TANRLKHARAQYLQEAREELDFPGLKEEFTLSRAESDLAERILHSPYFVLSAESVANY
                     SFVLADDELCLNLKKQLALHQQKREQEEARVLKKLSEKLSSELPCLRDAQDLLASACW
                     QFMLADFALRYCCAIPKLTRKKAIRVKGAVHLPLKLHLEELGRRWQSVDYYFSGEVSL
                     LTGPNMGGKTTVLRTAGQLCWLARLGIPLPCDEAELPLFDNIWYNQSETDSSADLSSF
                     GKEVVAFSEALRLEGRTLFLLDEFARGTNPAEGERLASAVLRHLAM"
BASE COUNT          871 a         1218 c         1247 g         1005 t
ORIGIN      
        1 ctttctgcac tttctggaac tcaagcgggc ggagaacatc ctgatcagct tcgacaccca
       61 aacggcgaaa acctcgcctg attatccgga ttcagccgcc gtggcaagcg gttttaagag
      121 agttgcagac ataaggagta tccatgtttg agtttattaa atcccttccc ggtttgtatt
      181 taagctgggt caaggcttgg ccgctgctgt cggcgatgat ccagttcgcg attttgggca
      241 ccttgggcga aatcatttcc aagtgggtca tccgaaagag tttccgctat cctttcacgt
      301 tcggattgac gctgtggaaa atgctcgtct gggcgcttct aggcgttgcg attaaatacg
      361 cctttcaggg cttcacgggt tttgtggaac atctggaggc gcattcctat ctgcctccat
      421 tgaacacatt tggcagggct tttgccatct ccgccttcat gaacctgcag tttgggccgt
      481 ttatggtgat cgtgcaccgg cttctggaca acctgggaga gcggaagcaa aactggaagg
      541 gtatccataa aagcatgctg gcgctgctct ggttctggat cccggcccac acggtgacct
      601 ttttgcagac accggacctg cgcatcgggc tggccgcgct gctgtcgctg gcgttgggac
      661 tgatcctggg catcttcaac tggcgccaag catagccttt ttcacttacc cgatgcgtcc
      721 caatccaccg cccgccccac ttttggtagc ggtgttggct tggtgcgcgg gtatcggcat
      781 cgccaggttt ctggacccac ccttccggcc cctgttggga ctgggggccc tgctgctggc
      841 cgcggcggta gttctgaaaa atgcccggcc agtattggcg ctactgctct gcctggtttt
      901 gggggctttg cgctggaacg cggcaaatga gccctccgcc ctggatgtgg ttttccgggc
      961 taagcgccac atccagcaag acgcggaatt cacggtcacc aaactgcttt cccgggaggc
     1021 ggggatatac gaaatccgct tggaagaact tgccggcgtg aaattgcgcg aacccctgag
     1081 cctgttctcg gaggaggaac ttataccggg acaggcatat acggcacttc tggaagtatt
     1141 gcccgggaaa caggacccca tactggatat gtttcccgcc cgacacagag cttatgtgcg
     1201 gcagtcactt acgccagttg acaaaccacc gcggatgttt ccggtagccc tctggagagt
     1261 gcggctgctg aaaaaccttg atgacaaact gggagcggat tccgaattcg ccaaagcgtt
     1321 tctgttttca gatacttccg ccaagggcca ataccgcgac caactcaccc gtagcggcat
     1381 gatccatctg atcgtggtaa gtggactgca tatctggttt atctacggaa tctgcatggt
     1441 gctgcttaac gctctgttcc ccaagcgggt agcagagctt gccttcctcg tgctgatcgc
     1501 cttttacgcc gcgcttaacc actggtcgcc accagtgacc cgggccatcc tgatgatcgg
     1561 cctgatgatc ctgggacgct ggcgcagcat acctttgggt ggcgcgcaac tgctggcgct
     1621 gagcctgctg atcatcaccg ccgtcagtcc tgaacagctc ttcaatatcg ggctgcagct
     1681 ctcgtatctc tgtatcggcg tgatcatcct gggcctgcct aaaatcgtct ggatcagcga
     1741 acagcggctt ccttcagacg ctctgcgggg aaggctgaac agatttttcg acctgctgct
     1801 tatgaatctt gtggtcggct tggcggtcct gccgctgacc ctgcactatc tcggaacggc
     1861 ctcacttaac gggattctgg gcaacctgct gggaattccg ctctcaggcg cactgctcac
     1921 cgtgagtttc atcgtgctgc tgattcccgg aggcaactgg ctgtgcaaca tctacatcgc
     1981 ctcataccgc ctgttattgt gtgctttcaa ccgatggaca gaatttgtgg cccggctgcc
     2041 cctttggctg gaaaacacct ggatcagtgg gctgcagctt tttggggcta tgctgttggt
     2101 ggtcagtatg ttgtatctgc tgcgtcggct tgagttcagt tggaaagtat tgcccgtcgc
     2161 agtgctggga ttggcgctgc ttttcctgcc ccaattccgc tcgaagccgg agggaggcat
     2221 ctacttattt gcgtgcggga caggggattg catcctggct tcatttccgg gtggcgagac
     2281 cctgctggtg gatacggggc ccaaattcca caacgctgaa aaaagctggg ctgccggcaa
     2341 actgctgccc tggctggccc gtaagaacat ttgtcgcatt gactggatgg tgctaacgca
     2401 tctggattcg gatcattcgg gaggttttcc cgatttggcc aaggcactga gcatcaacaa
     2461 cctggctgta accgacgaaa ccatccaaga cccaaaatgg gtggagtgga gcgagtccgg
     2521 atggcttgac gggatcaggg ttcacacgat ttccgacacc atctcatacc gaatgggtga
     2581 agccaggctg aagtttttgc atccggaccg cgattacttc acggagagct caaacagcgc
     2641 ttcgctggtc ttccgcctgg actatctgaa caccagctat ctctttacgg gagacgccga
     2701 tatagacgcg gaagcgcatc tgctggcttc atatcctgag gaactgaagg cggattttct
     2761 gaaagccgga caccacggca gccgcagttc cagcagccag gagtttgtgc gcgcggtgat
     2821 gccggacgag gtttggataa cggtttcaga ccgcaaccaa tggggatttc cccatccgga
     2881 accgctgaac gctttccgcc gttacgccag gcgtatcagc agcacctctt cgggcagtat
     2941 ccatcagcct ttcgcacaaa aagattgaca gggagggggc ggttcagctt tgctgaccgc
     3001 agagtctatt ataaagaagg atgtccatgc ccacacccag cgcccgggcc ttgggattgc
     3061 ccgcggtttt tgccctgatc gaactggcgg gggagttccc ggccgaggtg aagaaacagg
     3121 aattgaagcg cccgccgctg aagtttgcgg acgcgaagca ccgctatgcc aaattggagc
     3181 aggtgatcgc gctgctgcgg ggagaagatg gtgccctgcg tgacctgagg gagcttttcg
     3241 cccggattcc caagctcagt ctgccccagc gcggtggaaa agccgtcttt gccctgcacg
     3301 aactcttttt gctcaaagaa cacctttacc attacggtag gctgcatgcc tggctgcgcc
     3361 gcaaaggctg gcttgacacc ttcattctgc cggacctggg caaagttttc gcccttctgg
     3421 accccgacgg caccgaattg cccgcttttc acctttcagc ggcttattct gccaagctgg
     3481 gggaaatcct cagcgcccag ttcgaaacgg cgaaccgtct caaacacgca cgggcccagt
     3541 atctgcagga ggcgagggag gaactggatt ttcccggcct gaaagaggaa ttcacccttt
     3601 cccgtgctga aagcgacttg gcggagcgga ttttgcactc accatacttt gtcctgagcg
     3661 cggaaagcgt tgccaattac agctttgtgc tggccgatga cgaactctgc ctgaacctga
     3721 agaaacagct tgccctgcat cagcaaaaac gggagcagga ggaagcccgc gtcttaaaaa
     3781 agctctcgga aaaactcagc tccgaactgc cctgtttgag ggatgcccag gatttgctgg
     3841 ccagcgcctg ctggcaattc atgctggctg atttcgccct gcgttactgc tgcgccatcc
     3901 ccaagctaac acgcaaaaag gcgatccggg taaaaggggc ggtgcatctg ccgctcaaac
     3961 tgcatctgga agagctggga cgccgctggc agagtgtgga ttattatttc agcggtgagg
     4021 tttcgctcct tacggggccg aacatgggag gcaaaaccac tgtgctccga acagcggggc
     4081 aactttgctg gctggcgcgc ctgggcattc ccctgccctg tgatgaagct gagttgccgc
     4141 ttttcgacaa tatctggtac aatcagagcg aaacggattc cagcgccgac ctcagcagct
     4201 tcggcaagga agtggtggcc ttcagtgaag ccctgcggct tgaaggcagg accctgttcc
     4261 tgctggacga atttgcccgg ggcaccaacc ccgccgaggg agaaaggctg gcttcggcgg
     4321 tgttgcgtca cctggctatg g
//
LOCUS       DAOOWR010000129         4308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_20485_length_4308_cov_4.509523, whole genome shotgun sequence.
ACCESSION   DAOOWR010000129 DAOOWR010000000
VERSION     DAOOWR010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20485_length_4308_cov_4.509523"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PLB85_04165"
     CDS             <1..733
                     /locus_tag="PLB85_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOU72556.1"
                     /translation="IFVYDATVTIRDLDADLEFQLTPALHEFKFKYIDLAENIIQPGH
                     RYRIEVLVPGADSLIWAETTVPIAIEAAPDPYGTNPPGTGYSVDPNTENTMPFSLIDS
                     HYPVFASTGANAGKFNLMAEFYCREPFSTDLEFTSELMPTAHPDSSLADSYNAGQPPR
                     RLSIMYRFVSAPLPGLQDNYLVLENYSSAFIFYGRYRIRLLIVDDNYFNYNFMEEGYL
                     HGGIHNALGYFGSASGVDMYARIVK"
     gene            903..2477
                     /locus_tag="PLB85_04170"
     CDS             903..2477
                     /locus_tag="PLB85_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72557.1"
                     /translation="MKAIWKNWPTILLLGALAFAAKTLGAVDVMNVYFTGSSQLSHGF
                     QACQTVGDSLRLYDCEIMNDRVLIKLATVGPDGLCGPQQTIHQIIAESDWGDLSDTPL
                     AFSCKAGKLLSAFRAANRVIVCCTDDSGTSQHVFNTSGLNVEDSSFGPVLYIVDETLA
                     YLCSTRGAYPQINHEVYILNLSNNSQHLLYQGPGWLSHQVQSFADEYFLLWTLDGPDL
                     LVQNNNIIQTFPQGWFPASEGGGAGFTQTRKLCGDYFQTLAIDLIEGGPTSAYLVWLE
                     GSELHRQWVDSSIWPWQTPHFREVITLSDTSFSAISYQYNEWSFCHKSLVNGVFSDLA
                     GFPDLTGYPNAAFLRKMDADYTLAINRPTDTQYGFHLVDHPLGLVRDYSFDGMGVWAR
                     YCFNSERSVYLLGRAAYDAPWQVYAFHLELGLSTPDEEAPPVSAGITAFPNPFRSSCQ
                     IEIKTGVPQEASLEVFNLRGQKVARIFSGKLDRGESHHIWDGRDDDLRPVSSGIYLLR
                     LTTPEGSSSRRIMLAK"
     gene            2780..4024
                     /locus_tag="PLB85_04175"
     CDS             2780..4024
                     /locus_tag="PLB85_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72558.1"
                     /translation="MKAYFEHSLLLLALLLPALLAGQDYPQTYYLSGIDRHASGGGFG
                     GPDWWSVDCYTYTQAPGNTLLITQIGHLHRGWMYDFPPSEYYEVEDFDGELTLYPEYY
                     VITSPLTLQSNINFTRTRKMDYQGYCHEDDWYDYYSNRYHAWHSYNADMKLTRTVIKK
                     ARQNQTIEWLDCVCTLDSLGRRVEELTLSSADSLNWVNHRRRQYIYSGEQLPPGYQFE
                     KHNHQLPDYILNLVTVRVPYLNDGWIISQVTEQTANAQGMWYNPETWSLNIEVDTETG
                     EVTAEYCCTFSAEGLLTRIVIIDGDGIPSYDFHFAHTSDTAADDGVPQVTETLRAWPN
                     PAREKATLSLPGKLSGPVTLSTYNTRGQLIKEESRAVDAMNRDLGWQALDREGRPLDS
                     GIYLIRVRGTDFCQTARVMVLR"
BASE COUNT          975 a         1301 c         1125 g          907 t
ORIGIN      
        1 aatcttcgtt tacgacgcca ccgtcaccat ccgggacctg gatgccgacc tggagttcca
       61 gctcaccccc gccctgcacg aattcaaatt caaatacatt gaccttgctg aaaacatcat
      121 ccagcccggc caccgctacc ggattgaggt gctcgttccc ggcgcggatt cgctgatttg
      181 ggccgaaact accgttccca tcgccattga ggcagcgcct gacccctacg gaaccaatcc
      241 ccccggaacc ggctattcgg tggaccccaa taccgaaaac acaatgcctt tcagcctcat
      301 cgacagccat taccctgtct tcgcctccac cggggccaac gccggcaaat tcaacctgat
      361 ggcggaattc tactgccggg aacccttcag cacagacctc gaattcacgt cggaattgat
      421 gccgacggcg catcccgact ccagcctggc agacagctac aacgccggcc agccaccccg
      481 ccgcctcagc atcatgtacc gctttgtctc agcgcccttg cccggtttgc aggataacta
      541 cctcgtgctg gaaaactact cctctgcctt catcttctac ggacgctacc gcatacgcct
      601 gctgatagtt gacgacaact acttcaacta caatttcatg gaggaaggct acctccacgg
      661 cggaatccac aacgccctgg gctatttcgg ctccgccagc ggagtcgata tgtatgcccg
      721 catcgtgaaa tagcagacgc ccagaaagcc cgatagggct tcactccggg atttcctgac
      781 agctttggcg gcctgcctgt cggttacaaa aagacttcca ttcctttccc gactgatatt
      841 ctttgaataa tggcctgctt attgctatat tggatggata gatttcccag ggaggtgaac
      901 aaatgaaagc tatttggaaa aactggccca ccatccttct tctgggggcg ctggctttcg
      961 cggccaagac cttgggagcg gttgatgtga tgaatgttta tttcaccggc agttctcagc
     1021 taagccacgg attccaggcc tgccagaccg tgggggacag cctgcgcctg tatgactgtg
     1081 aaatcatgaa tgaccgcgtc ctcatcaagc tggccacggt cggccctgac ggactctgcg
     1141 ggccccagca gacaatccac caaatcatag ccgaatccga ttggggcgat ttatctgata
     1201 ctcccctggc cttttcctgt aaggccggca agctgctttc cgccttccgc gcggccaacc
     1261 gggtcattgt ctgctgcact gatgacagcg gcaccagcca gcatgttttc aatacctccg
     1321 ggctgaatgt cgaggattcc agcttcggcc ccgtcctcta catcgtggat gaaacgctgg
     1381 cctacctctg ctccaccagg ggagcctatc cccagatcaa ccatgaggtg tacatcctga
     1441 acctgagcaa caacagccag catctcctgt accaaggccc gggctggctt tcccaccagg
     1501 tccaatcctt tgcagatgaa tactttctcc tctggacttt agacggaccg gacctcctgg
     1561 tgcaaaacaa caacatcatc caaaccttcc ctcagggctg gttcccagcc agcgaaggcg
     1621 gaggagcggg cttcacgcag accaggaaac tctgcggcga ctactttcag accctggcca
     1681 tcgacctcat tgaaggcggg cccacctcag cctacctggt ctggctggaa gggagcgaac
     1741 tgcatagaca atgggtcgac agctccatct ggccctggca gactccgcat ttcagggaag
     1801 taataaccct cagcgacacc agcttttcag caatatctta ccagtataac gagtggtcat
     1861 tctgccacaa atcccttgtt aatggcgtat tcagcgacct ggctggcttc cctgacctga
     1921 ctggataccc gaacgcggct tttctgagaa agatggacgc agactacact ctggccatca
     1981 accgaccgac cgacacgcag tacggcttcc atctggttga ccatcccctg ggcctggtga
     2041 gggattacag ttttgacgga atgggtgttt gggcaagata ttgctttaat tcagaacgca
     2101 gcgtctatct gctgggcagg gccgcgtatg acgctccctg gcaggtttac gccttccatc
     2161 tggaacttgg cctctcaacc ccggatgaag aagccccgcc agtttcggcg ggaatcaccg
     2221 ccttccccaa ccccttcagg tcatcctgcc aaatcgaaat caaaaccggg gtcccgcagg
     2281 aagcgagcct ggaagtcttc aacctcaggg gtcagaaagt ggcgcggatt ttctctggca
     2341 aactggacag gggcgaaagc catcatattt gggacggccg cgatgacgac ctgcgaccag
     2401 tttccagcgg gatatacctg ctccggctga caactccgga aggctcttcc agccgcagga
     2461 taatgctggc caagtgattt acacaaggta aagccgctcc ctaacaaggc cacgagcctt
     2521 ttccctgctc cccgttagcg cctcctgatt cgggttcggg cagcctctcg cataatgccc
     2581 gcaccggatg cgggcatctt ccgggaggcg ggcaagaggc aactttccgg gcgttaagga
     2641 ggctcggtct gacatttgcc gggcggatgg aatggtgttt gcatataatt atattactga
     2701 gaaaatgatg ccaacaagcc gggatgccac cgagcgatga actctggcct ggaagcggga
     2761 catccggcgg ggaggaacga tgaaggcata ttttgaacac agcctgttgc tgctggcgct
     2821 gctgctgccg gcattgctgg cagggcagga ttatccccag acctattacc tgagtggcat
     2881 tgaccgccat gcttcgggcg gaggattcgg cggtccggac tggtggtcgg tggattgtta
     2941 cacctacaca caagccccgg gcaacactct gctgattacc caaatcggcc acctccaccg
     3001 gggctggatg tatgactttc cccccagcga atattatgaa gtagaggatt ttgacggcga
     3061 actgaccctg tacccggaat attatgtcat caccagcccc ctgaccctgc agtcgaacat
     3121 caacttcacc cggacccgca agatggatta tcagggctat tgccacgagg atgactggta
     3181 cgattactac tccaaccgct accacgcctg gcacagctac aatgccgata tgaagctgac
     3241 ccgtaccgtg ataaaaaaag cgcgccagaa ccaaaccatt gagtggctgg actgcgtttg
     3301 caccctcgac agcctggggc gacgggtgga ggaactcacc ctcagctctg ccgattcact
     3361 caactgggtg aatcaccgcc gccggcaata tatctattcc ggtgagcagc ttccccccgg
     3421 ttaccagttc gaaaaacaca accatcaact gcccgactac atcctcaatt tagtgaccgt
     3481 tcgcgttccc tatctcaatg acggctggat catctcccaa gtgacagagc aaacagccaa
     3541 tgcccagggc atgtggtaca atccggagac ttggagctta aacatcgagg tggataccga
     3601 aacaggagaa gtcaccgccg aatattgttg caccttcagc gcggaggggt tgctgacaag
     3661 aattgtaatt atagatgggg acggcattcc cagctatgat ttccatttcg cccacacttc
     3721 ggacaccgca gcggatgacg gcgtgcctca ggtgaccgaa accctgcggg cctggcccaa
     3781 cccggcgagg gaaaaggcga ctctgagcct gcccggcaag ctatccggcc cggtaaccct
     3841 gagcacctac aacacccggg gacaactaat caaagaggaa agccgcgctg tggacgccat
     3901 gaaccgcgac cttggctggc aggcgctgga cagggaaggc cgacctttgg acagtggaat
     3961 atacctgatt cgcgtccggg gcacggattt ctgccagacg gccagggtga tggtcctgcg
     4021 gtagcgttta gggccccgct ttccgtcagc ctcagcggaa gaacgttggg cggaataatt
     4081 ttccggactg aacgagtcca taaggcgaca taacgattgc gagaggagcg taagcccctt
     4141 gtacactgga ttccgggtca atcctggaat gacgtgcaag acgttggccg attgaacata
     4201 agcctaaaaa aggcgggagt ggatcccgcc tttcatattg cctttatttg gctgcgtcag
     4261 tgggcttcgt gcttttgggt gacctgttcg accacctggc cgctgctg
//
LOCUS       DAOOWR010000130         4274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_20682_length_4274_cov_3.602275, whole genome shotgun sequence.
ACCESSION   DAOOWR010000130 DAOOWR010000000
VERSION     DAOOWR010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20682_length_4274_cov_3.602275"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLB85_04180"
     CDS             <1..518
                     /locus_tag="PLB85_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU72559.1"
                     /translation="VPLGKSSSVHWVKATRDRMYAETGLKPSNEELSEKMNITEKSIE
                     QLQDQMVETTSYDELISNGDFQEFTTRDMIADDDLVDPQQIYHRERLQHKINRAIEKL
                     DQREADIIRTYYGLNAGQETQNFARIAEKMGLSRERVRQIQKEALKKILSDLKPEEDA
                     LVDEFIEKYST"
     gene            546..1448
                     /locus_tag="PLB85_04185"
     CDS             546..1448
                     /locus_tag="PLB85_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOU72560.1"
                     /translation="MYKNFDEMVSAVKASRRGTVIISAAQTESVLDAAILAKQEDLAD
                     CLLVGDKPKILALLEKMAPQHTNSFEIVDIGPDLVKAAKVSVQLAREGKGDIILKGKT
                     DSGQLLRAVLDKENGLRVSDVISDVLAYEHPEGLKLLTDGGVNLVPDLKEKVTIVKNA
                     VQVAHSLGSELPKVAMITAVEKVNPKMPATLDAAIISRMNARNQITGCVVEGPLAFDI
                     AVDINAARIKGIESPVAGVADVFVVPNIEAGNVFGKMLTYYCGFRVAHVVVGTKAPIL
                     IPSRADRGELKMLCMAMALACMNK"
     gene            1445..1909
                     /locus_tag="PLB85_04190"
     CDS             1445..1909
                     /locus_tag="PLB85_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOU72561.1"
                     /translation="MILRVLQVGKTKEGWLKEGISEYMKRLAPMMRLEVTDIADVSLK
                     TAGSPDAVKAREAALCLKRLAPEDFVILMDERGEAKTSLEFAEFLNNLSSEKSVVFLI
                     GGVYGAHESLKTRANACLSLSALTFTHQMARLVLVEQIYRALMINNGRSYHY"
     gene            1928..2083
                     /locus_tag="PLB85_04195"
     CDS             1928..2083
                     /locus_tag="PLB85_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOU72562.1"
                     /translation="MTYTMLATTMQNFTQSLLILLQGMGGIFLFMGLFYGLIVALEKI
                     FQGKKEP"
     gene            2080..>4274
                     /locus_tag="PLB85_04200"
     CDS             2080..>4274
                     /locus_tag="PLB85_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72563.1"
                     /translation="MNKFYAVLAFLLIACSAFASVRVLERSANQILLEYRLESYKLSE
                     VDDMLRVDVAGMDYDIRSGAPLLPLEEARIALPPGGEVNAVLISSSQSQATLSKRLLP
                     VPEVRQNEDISSHVYKIDESLYLNGAKSLLTTSVSTFRGLDFCALHIRPFAYDGRFGL
                     TVTDQAIIRIDISGDTSHQSLPQSDELMDLFSETLINPGQARQWQKQTSNAVFYSDFS
                     RADYWFRLETDREGMFRIDPDQLTGFPLADIDPRSFRLFSNGGKLLPFTIINPGNEFV
                     EVPIRVVGEEDGSFDPWDYIVFYGTTRDGTEKNISLQNNQTYYNPYSGNTVYWLTFAG
                     EFGGDPLRMVTLPQEHSWYAQTSKSRELTRLETETHRREIIGFDWYMTRMFGNSNSEY
                     EFELQLPDLDPSADQALSFIIRQEDTSIDSLWHNINVFVNGVPVQADTLGSTNFKWRG
                     LGDYLFYKNVSSFVSGQNTVRIRVLRNSTDNLFLNWITVDYTRTINKGSGQNLVNQMA
                     LNYEVPIRYNLSGSADTRVYRVNGLADVKMVPLMSSDGQNYFVSTGFSNTKYWLSNDH
                     ELHSPVIVGLVETNDLARIQSQYDNVIITADEFQTQALSLADMYLQDYGKRSLVVKQS
                     DIFNQFNGGHPDPVALRQFLRYAWYNYPTPKISSVTLIGLGTSDWRNNSKQAQGKNKL
                     IVFQRGSIASDDYFVMLTQSLYPELTIGRYPVTNSNELAIMLSNRENYVR"
BASE COUNT         1060 a         1183 c         1113 g          918 t
ORIGIN      
        1 gcgtgcctct gggcaaatca agctctgtcc actgggtgaa agccactcgg gaccgtatgt
       61 acgccgagac cgggctcaag ccatccaatg aggaactgag cgaaaagatg aacatcacgg
      121 agaaatccat cgaacagctt caagaccaga tggtcgaaac gacctcatac gacgaactta
      181 tctccaacgg tgatttccag gaatttacca cccgcgacat gatcgctgac gacgatttgg
      241 tcgaccctca gcagatttat caccgcgaac gcctgcagca taagatcaac cgggccatcg
      301 aaaagctgga ccagcgcgaa gctgacatca tccgcactta ttatggcctg aacgccgggc
      361 aggaaaccca gaactttgcc cggatcgctg aaaagatggg gctttcgcgg gaaagggtgc
      421 gccaaatcca gaaagaagcg ctgaagaaaa ttctatccga cctcaagcca gaggaggatg
      481 ccctggtgga cgaatttata gaaaaataca gcacctgagc ctgtctcggg tagcggagaa
      541 cagacatgta taagaacttc gacgaaatgg tgagcgccgt gaaagccagc cggcgcggca
      601 ccgtgatcat ttcagccgcg caaacggaat ccgttctcga cgcggcaatt ctggccaaac
      661 aggaagacct ggccgactgc ctgctggtgg gcgacaaacc caaaatccta gccctgctgg
      721 aaaagatggc gccccagcac actaactcct ttgagatcgt ggatatcggc cccgacctgg
      781 tgaaagccgc caaagtgagc gtccagcttg cccgggaagg caaaggcgac attatcctga
      841 aaggcaagac tgactccggc cagcttctta gggctgttct ggacaaggaa aacggcttgc
      901 gcgtgagcga cgtgatctct gacgttctag cctacgaaca tcccgaaggc ctgaaactgc
      961 tcaccgacgg cggggttaac ctggtcccgg acctcaagga gaaggtgacg atcgtgaaaa
     1021 acgctgtcca ggtggcccac agcctgggtt ccgaactgcc aaaggtggcg atgatcaccg
     1081 ctgtggaaaa ggttaacccc aaaatgccgg ccacgcttga cgccgccatc atctcaagga
     1141 tgaatgcccg gaaccagatc acaggctgtg tcgtggaagg ccctctcgct ttcgacatcg
     1201 cggtggacat caacgccgcc aggataaagg gcatcgaatc ccccgtcgcc ggggtcgcgg
     1261 atgtcttcgt tgtgcctaac attgaggctg gaaacgtctt cggtaagatg ctcacctact
     1321 attgcggatt tcgcgtggcg cacgtggtgg tgggcaccaa agctcccatc ctcatcccct
     1381 cccgggcaga tcgcggtgag ctaaaaatgc tctgcatggc gatggccctg gcctgtatga
     1441 acaagtgatc ctgcgcgttc ttcaggttgg caagaccaag gaaggctggc tgaaggaagg
     1501 aatctccgaa tacatgaaac ggctggcccc gatgatgaga ctggaggtga cggatatagc
     1561 tgacgtgagc ctcaaaacag ccggcagccc cgacgccgtg aaagccaggg aagccgctct
     1621 gtgcctgaaa cgccttgcac cggaagattt cgtgatcctg atggacgaac gtggcgaagc
     1681 taaaacttcg cttgaatttg cagagttttt gaataacttg tcttcagaga aatcggtcgt
     1741 tttcctgatc ggcggagtgt atggcgcgca cgaaagcttg aaaacccggg ccaatgcttg
     1801 cctgagcctg tcggctttga ctttcacgca ccagatggcg cggctggttc tggttgaaca
     1861 gatctaccgc gcgctgatga tcaacaatgg tagaagctac cactattaaa agtttcagag
     1921 gcctccgatg acctatacaa tgcttgccac gacgatgcaa aacttcacgc agtccctact
     1981 gatccttctg caggggatgg gtggaatctt cctgtttatg ggcttgtttt acggcctgat
     2041 cgttgccctg gagaaaatct tccagggaaa gaaggaacca tgaataagtt ctatgccgta
     2101 ctggctttcc tgctgattgc ctgctcagct tttgcctccg tgcgggtgct ggaacgttca
     2161 gccaaccaga ttctgctgga gtatcggctg gaaagctata aactgagtga agtggatgat
     2221 atgcttcggg tggacgtggc cgggatggat tatgatatcc gctccggcgc tccgttgctg
     2281 ccgctggaag aagctcggat tgcgcttcct cccggaggcg aggtcaacgc cgtgctgatc
     2341 agctccagcc agagccaggc cacactttcc aagcggttgt tgcctgtgcc ggaagtaagg
     2401 cagaacgaag acatctcctc gcatgtttac aaaatcgatg aaagcctata tctgaatggc
     2461 gccaaatcct tgcttacaac ctccgtctcc acttttagag gtttggattt ttgcgctctg
     2521 cacattcgcc cctttgccta cgacgggcgc ttcggactca ccgtcaccga tcaggccatc
     2581 atccgaatcg acatttccgg cgatacatcc caccagagcc tgccccagag cgacgaactg
     2641 atggatttgt tcagtgaaac cctgatcaat cccgggcaag cccgccaatg gcaaaaacag
     2701 accagtaacg cggtttttta ctctgatttt tcccgggcgg attactggtt tcggctggaa
     2761 acagaccgcg aaggaatgtt ccgcatcgac ccagatcaac tgacgggatt tccacttgcg
     2821 gatatcgacc cccgttcgtt ccgcctcttc agcaacggag gcaagctgct tcccttcacg
     2881 atcatcaacc ccggcaacga gtttgtggag gtgcccatcc gcgttgttgg cgaagaagac
     2941 ggcagcttcg acccctggga ctacatcgtt ttttatggca caacccggga tggcacagaa
     3001 aagaacatca gcctgcaaaa taaccagaca tattacaacc catattccgg aaacactgtt
     3061 tattggctca cctttgccgg ggaattcggc ggagaccctc tgcgaatggt aactttgcca
     3121 caggaacata gctggtatgc ccagacctcc aaatcccgtg aattgacccg cctggaaact
     3181 gaaacccatc gccgtgagat aataggcttc gattggtata tgacccgcat gttcggcaac
     3241 tccaattccg agtacgagtt cgagcttcag ttgccagacc tggatccctc cgctgaccag
     3301 gcccttagtt tcatcattcg ccaggaagat acaagtatag actccctctg gcacaatatc
     3361 aatgtctttg tgaacggagt gcccgtgcag gcggatactc tgggctccac caacttcaaa
     3421 tggagaggct tgggagatta tctgttttac aaaaatgtct ccagcttcgt cagcggccaa
     3481 aacaccgtcc ggataagagt gctgagaaat tccaccgaca accttttcct gaactggatc
     3541 accgttgact acacccgaac cattaataaa ggcagcggtc agaatttagt gaaccaaatg
     3601 gccctcaatt atgaggtccc catccgctac aacctttccg gcagcgccga cacccgtgtg
     3661 taccgggtaa atggcctggc tgatgtgaaa atggtgccgc tcatgagttc cgatggacag
     3721 aattactttg tgagcactgg cttcagcaat accaaatact ggctcagtaa tgatcatgag
     3781 ctccacagtc ccgtgatcgt tggtttggtg gaaaccaacg atcttgccag gatccagtct
     3841 caatatgaca atgtgatcat cacggcggac gaatttcaaa cccaggccct gtccttggcg
     3901 gacatgtatc ttcaggacta tggcaaacgc agtctggtgg tgaagcagag cgacatcttc
     3961 aaccagttca acggcggcca tcctgacccc gtggccctgc gccagttcct gcgctacgct
     4021 tggtacaact accccacgcc caagataagc tccgtgaccc tgatcggcct tggcaccagc
     4081 gactggcgca acaattccaa gcaagcccag ggaaagaaca aacttatcgt cttccagcgt
     4141 ggttcgatcg cttcggacga ctattttgtg atgctcaccc agtcattgta tcccgaactt
     4201 acgattgggc gctatcccgt aaccaattcc aacgaactgg cgattatgct atcgaatagg
     4261 gaaaactatg tacg
//
LOCUS       DAOOWR010000131         4203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21187_length_4203_cov_3.916345, whole genome shotgun sequence.
ACCESSION   DAOOWR010000131 DAOOWR010000000
VERSION     DAOOWR010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21187_length_4203_cov_3.916345"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PLB85_04205"
     CDS             complement(<1..684)
                     /locus_tag="PLB85_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72564.1"
                     /translation="MKRFLLPLAFLLVLIPLWGQYNERDILNQQAYQMLGQRQFAEAE
                     KIFLQVLEKYPDDANSVLQLLNIYFQTSQLDKAENLLRQHRRILPANQATEQEILLLV
                     MQGRPDDAWNLGQTQLSRMNHSESTYRLLASYFERRGFYDHVLRLYEEARARRGNPDL
                     FRLEIANAALNYRRFEQALREYLSFLEKNPSNIFFVNNQCKTILREDPELIATIGEFA
                     ETSANPIIKE"
     gene            complement(681..3899)
                     /locus_tag="PLB85_04210"
     CDS             complement(681..3899)
                     /locus_tag="PLB85_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72565.1"
                     /translation="MNEFLSKLHNFKRGLNLRILARALVISLLCLVVGLHAYFLVWLN
                     TPAQSPTLVYLNYALRALMVGLVLFLIYKGIRGFYDNRSAARWLDRQTEHEDDLYQNL
                     WELKQQKESEPVLDALARQAQSRLKASSYRLPRLFQPSQWWLILFVLAGIGSVWALGW
                     NEFRYALNQFRAVKADAIEYKKTVELSPGNVTIGKGQQLLIRVLEPDTRLKHRLFYRW
                     DKQWRELGMTDNSYSFPALEYSIEYYVKNEVASSPVYKVVCLDEPFARSWSLYYKYPA
                     YTGLGSRSDSLSYGNIEAYKHSEVIISLGTNIPVEKAVMRFSDGTSQALQRVEETSFS
                     GRITVTAPKTWYLELTDALGRKSRPEEKTICVIPDNAPEIRIIYPGEDVLLDQSLLLP
                     LIISASDDFGLRELSLHYQINDRPAQQTTLRSLIGSKLFTLDHTLDLKGFGLLPGDTV
                     TYWAQVWDNSPDRQSAQSVKFKARFPSIEEIYREIERQAQSSTQDLQSTLKEAKDLQK
                     DFEQKRRELMKNDKLDWEDKKQLEHMLDTQQQLTEQVENVAQDFQRLIDQMQRNDAVS
                     SETLQKMQKIQELMEEISTDELREAMSKFEQALMNVKPEDLRKAMENMKFSMEDFSKK
                     IDQTLALLESIKKEQALDKALQISKEMEQMQSALKDKTGDPKQNTDRLAQEQENISDK
                     LDNLQKALDEADKLLDPAKDKQLKQEMKDLKQDMKSGQLQKNMRQSQLQLQQNQRSQS
                     GQSQSQALEKMRQYTRRLSEMKESMGGGSMQEVTSAMQKAIRELLIFSKQHEELRGRY
                     ANDPYPIVNDLIAQYEGVQVSLNRLFSVPQVTMFLPPKFYIDLNDTNRGYRDVFINVN
                     EMQYAQIPAQMEIIQKGLNLMIYDLMQTLNNPSGGGGGGGMQSLMQMLGQMGEEQMAL
                     NMLSEQLMMQLQQQGGRMDAGMQQQIQKLASDQERLAENLKRALQNNPEAQKQGNAIK
                     KIIEEAEAVSRQLRNNQLSQDILNRQENIISRLLDAQRSINKRDQSERRQAQSADPTI
                     RGADVNTDYDTLRRKAMLEDSYRLFPASYQQVIIKYLKLLNE"
BASE COUNT          740 a         1094 c         1247 g         1122 t
ORIGIN      
        1 ctctttgatg atgggattgg cgctggtttc ggcgaattca ccgatggtgg cgatgagttc
       61 ggggtcctct ctgaggatgg ttttgcactg gttgttcacg aaaaagatat tggaggggtt
      121 tttctccaga aaagagaggt attccctcag cgcttgctcg aaccggcgat agttgagcgc
      181 ggcgttggcg atttccagcc ggaaaaggtc aggattgccg cggcgggccc tggcctcttc
      241 gtaaagccgc aaaacatggt cataaaaacc ccgacgctcg aaataggatg ccagcagccg
      301 ataggtgctt tccgaatggt tcatgcggga aagctgcgtc tgaccaaggt tccaggcatc
      361 gtccggcctt ccctgcatca ccagcagcag gatttcctgt tccgtggcct ggttggctgg
      421 caaaatgcgt cggtgttgtc gaagcaggtt ttccgctttg tccagttgcg aggtttggaa
      481 atagatgttc agcagttgca gcacgctgtt ggcgtcgtcg gggtattttt ccagcacttg
      541 caggaagatc ttttccgcct ccgcgaactg gcgctgtccc aacatctggt aggcctgctg
      601 gttgaggatg tcacgctcgt tgtactgtcc ccaaagcggt atcagcacca gcaggaaggc
      661 aagcggtagc aggaaacgct tcattcgttg agcagcttga ggtatttgat gatcacctgc
      721 tggtaggaag ccgggaagag gcggtatgaa tcctccagca ttgctttgcg gcgcagggtg
      781 tcataatccg tgttcacgtc cgcgccccgg atagtggggt cagcgctctg ggcctggcgg
      841 cgttcgctct ggtcgcgttt attgatgctg cgctgggcgt ccagcagccg cgaaatgatg
      901 ttttcctgac ggttcaggat gtcctgggaa agctggttgt tgcgcagttg gcgagaaaca
      961 gcctccgctt cctcgatgat ctttttaatg gcgttgccct gtttctgggc ttcgggattg
     1021 ttttgcaggg cccgtttcag gttttcggcc aggcgttcct ggtccgaagc cagtttctgg
     1081 atttgctgct gcatcccggc atccatgcgt ccgccctgtt gctgaagctg catcataagc
     1141 tgctccgaaa gcatgttcaa agccatctgt tcctcaccca tttgccccag catctgcatc
     1201 agcgattgca tcccgcctcc gcctcctccg ccggaagggt tgttcagggt ctgcatcagg
     1261 tcgtatatca taaggttcag ccctttctgg atgatttcca tctgggcggg tatctgcgcg
     1321 tactgcattt cgttaacgtt aatgaagaca tcgcggtagc cgcggttggt gtcgttgagg
     1381 tcgatataga atttcggcgg caggaacatt gtcacctggg gaacgctgaa cagccggttc
     1441 agagagacct gcacgccttc atactgggcg atgaggtcgt tcacgatggg gtaggggtcg
     1501 tttgcatagc ggccccgcag ttcctcatgc tgtttggaaa agataagcag ttcgcggatg
     1561 gccttttgca tggcggaggt cacttcctgc atgctgccgc cgcccatgga ttctttcatt
     1621 tcgctcagcc gccgcgtgta ttggcgcatc ttttccaggg cctgggattg tgattggccg
     1681 gactgggagc gctggttctg ctgaagctgt agctggctct gccgcatgtt tttctggagc
     1741 tggccgctct tcatgtcctg cttgaggtct ttcatttcct gcttcagttg tttgtctttg
     1801 gcggggtcca gcagtttgtc agcttcatct agggcctttt gcaggttgtc taatttgtcg
     1861 ctgatgtttt cctgttcctg ggccaggcgg tccgtgttct gtttaggatc gccggtcttg
     1921 tccttcaggg cgctttgcat ctgttccatc tctttggata tctgcaaagc cttgtccagc
     1981 gcctgttctt tcttgatgct ttccagcagg gcgagggttt ggtcgatttt cttgctgaaa
     2041 tcctccatcg agaatttcat gttttccatc gccttgcgca ggtcctccgg tttcacgttc
     2101 atcagcgcct gctcgaattt gctcatcgcc tcacgcagtt cgtctgtgct gatctcttcc
     2161 atcagttcct ggattttctg cattttctgc agggtttcgg aggaaaccgc gtcgttgcgc
     2221 tgcatctggt ctatcaagcg ctgaaagtcc tgcgccacgt tctccacctg ctcggtgagc
     2281 tgctgctgcg tatccagcat atgttccagc tgttttttgt cctcccaatc cagtttgtca
     2341 ttcttcatca gttcgcggcg tttctgctca aagtcctttt gcaggtcctt ggcttccttc
     2401 agcgtgctct ggaggtcctg agtgcttgac tgggcctggc gttcgatttc gcggtagatc
     2461 tcctctatgg aggggaaacg ggccttgaat ttgacggatt gcgcgctctg acggtcgggc
     2521 gaattgtccc aaacctgcgc ccagtaggta actgtgtcgc cagggagtag gccgaagcct
     2581 ttcaggtcga gggtgtggtc cagcgtgaag agcttggagc cgatgaggga acgcagcgtc
     2641 gtctgctgtg cagggcggtc gtttatttga tagtggaggc tcagctcacg caggccgaaa
     2701 tcgtcgctgg cggagattat cagcggcagc agcaggcttt ggtccagaag cacgtcctcg
     2761 ccgggataga tgatgcggat ttccggggcg ttgtccggta tcacgcagat ggttttttct
     2821 tccggccggg atttgcggcc cagggcgtcg gtgagttcca gataccaggt tttgggtgca
     2881 gtaacggtga ttctgccgct gaaggaagtt tcctccaccc gctgcaaggc ctgggaagtt
     2941 ccgtcggaaa agcgcatcac ggctttttcc acggggatgt tggtgcccag ggagatgatg
     3001 acctcgctgt gcttgtaagc ctcgatgttg ccgtagctga ggctgtcgga acggctgccg
     3061 aggcccgtgt aggcgggata tttatagtaa agcgaccagc tccgtgcaaa gggctcatcc
     3121 aggcaaacga ccttgtaaac ggggctggag gccacttcgt ttttcacgta gtattcgatg
     3181 ctgtattcca gcgcaggaaa gctgtagctg ttgtccgtca ttcccagttc gcgccattgt
     3241 ttgtcccagc ggtaaaagag acggtgcttc agccgggtgt ccggctccaa aaccctgatc
     3301 aagagctgct ggcctttgcc gatggtcacg ttgccagggc tgagttcgac ggttttttta
     3361 tactctatgg cgtccgcttt caccgcccgg aactggttca gagcatagcg aaactcgttc
     3421 cagccgaggg cccaaacgct gccgatgccc gccagcacaa agaggataag ccaccactgg
     3481 ctgggctgga aaagcctggg gaggcggtag gatgaagctt tgagccggct ctgcgcctgt
     3541 ctggccaggg cgtccagaac cggttcgctt tccttttgct gcttcagttc ccagaggttt
     3601 tggtaaaggt cgtcctcgtg ctcggtctgg cggtccagcc agcgggcggc ggagcggttg
     3661 tcataaaaac cccggatgcc tttgtagatg aggaaaagga ccaaacccac catcagcgcc
     3721 ctgagcgcgt aattcaggta aaccagcgtc ggcgactgcg cgggggtgtt caaccacacc
     3781 agaaagtagg cgtgcaaacc cacaacgagg cagagcagcg aaatcaccag cgcccgcgcc
     3841 agtatccgca ggttcaagcc ccgcttgaag ttgtgcagtt tgctcaagaa ttcgttcatt
     3901 cgaccttatc cataacagag atcaaatctg caggcgccgg ttttcgtcaa tcacaatttg
     3961 gcctgggggc gtgtttcatc tccgcgaaga aagggattcg ccgttggggc gtttttgatt
     4021 tttggcttga ccggtagcgg atttgttacc ctctataaat ggatgcggca gggaaggaag
     4081 ccggaagatt tgggggttga aggaggtttt ttgatagatt cagcttggtt tcgccctttg
     4141 cccctttcca tgcgcctcct tttcgcctcc cgctgacttc ccgtttggaa ggcgggaact
     4201 cta
//
LOCUS       DAOOWR010000132         4185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21325_length_4185_cov_3.462470, whole genome shotgun sequence.
ACCESSION   DAOOWR010000132 DAOOWR010000000
VERSION     DAOOWR010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21325_length_4185_cov_3.462470"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="PLB85_04215"
     CDS             <1..1101
                     /locus_tag="PLB85_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72566.1"
                     /translation="GLNGDLHAVDHNGIPVPGFPVNLGSAYFSEMAAADLDGDCLMEI
                     IVGTNNGNLHVLDGTGNYKPGFPISIFGAISGAPTVLDNGRIALGTNTRMLLVSPTGE
                     ILVDKQIPSAMASSPVLADLNGDGALEIVFVTLGGILCAVDQNGDYLSGFPLNVGAVC
                     NTPPLIANLDDDPAPEILVSSNINSVYAYKTDGSMLDGFPFITSYNGTTPGTICDLDS
                     DGILKLVSGYSTGVLVLNLRRAAGGCMPWVTYRGSLKRQGSHAAAILPSSDPTVPALS
                     DKLLQNYPNPFNPSTTIAFQTAQNGEARLTVFNLKGQFVRELNSGYLPKGDHSVLWDG
                     RDDCGNAVASGLYFYRLQTREASITKRMLLMK"
     gene            1174..2094
                     /locus_tag="PLB85_04220"
     CDS             1174..2094
                     /locus_tag="PLB85_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU72567.1"
                     /translation="MPKVLIATEKPFAAAAVQKIKAELDAAGYAHAWLENYTDPKDLH
                     TAVADTDALIIRSDKVDEAVFAAAPKLKIVVRAGAGYDNIDLAAATAHNVVAMNTPGQ
                     NSNAVAELALAMMIYMARGKFNGKSGSELAGKKLVLYGFGFVPRFLAKMAKGVGMEVF
                     AYDPYISPEIMAKEGVTTLNTVQEIFSTGDYISLHIPATAETKKSINWDLLSLMKDNC
                     IMVNTARKEVIDEDSLLKAFEEKAKFRYVSDVEPDCSAKLAELYPDRSYWTPKKMGAQ
                     TGEANVNAGVAAAKQIIAFLEKGDTTFKVN"
     gene            complement(2180..2449)
                     /gene="rpmA"
                     /locus_tag="PLB85_04225"
     CDS             complement(2180..2449)
                     /gene="rpmA"
                     /locus_tag="PLB85_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOU72568.1"
                     /translation="MAHKKGVGSSRNGRDSNPKYRGVKKYGSEYVVAGNIIVRQKGTK
                     IHPGANVGMGRDYTLFSLIEGHVKFETRGASNRKFVSVIPISSEA"
     gene            complement(2461..2775)
                     /gene="rplU"
                     /locus_tag="PLB85_04230"
     CDS             complement(2461..2775)
                     /gene="rplU"
                     /locus_tag="PLB85_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOU72569.1"
                     /translation="MYAIVDIKGFQYRAEANAILRVPLLNTAEPGQSLEFDRVLLVRN
                     EDEIMVGQPVVEGAAVLAEVIEHGKGKKKVIYHAKRRKGYRVKKGYREQYTEIKVTGI
                     RI"
     gene            3169..4161
                     /locus_tag="PLB85_04235"
     CDS             3169..4161
                     /locus_tag="PLB85_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72570.1"
                     /translation="MSPLHLVGEGHFAGKAAVHSELGLTLGTINDSTGERYFLKPQLK
                     RTHCKAFTRSENRALYSVNDLLLYAEGDNVADWSASTKGQGLIRSLDLASDGSAFLLQ
                     ETAGPDTRQLKVRVLEAGTGDLDYEIAYQVDTGLNATTVLPLGHRIFALYEVGTLKVY
                     QVYSGTSTLLASWPLNISDPGQYDLSNSTLALVPADDDVLDIYLWLRAIGTEPNQSGQ
                     VLHARTILTVPNDDPGLPPVLQPSLTAWPNPAFGNVNIKLDHASGSQPVLEVYNLRGQ
                     KVRVLQPEKTASGSSFECIWNGLDDSGQPAAKGVYFLRATLPGLNIPMKRICLY"
BASE COUNT          947 a         1232 c         1054 g          952 t
ORIGIN      
        1 gggctcaatg gcgatctgca tgccgttgat cataatggca taccggttcc gggtttcccc
       61 gtaaacctcg gttccgccta cttttcagag atggcagctg ccgacctgga cggcgactgc
      121 ctgatggaaa tcatcgtcgg caccaacaac ggcaatttgc acgtcctgga cggcactggc
      181 aactacaaac ccgggttccc catttctata ttcggcgcca tatccggtgc accaactgtg
      241 ctggacaacg gcagaatcgc cctgggaacg aacactcgga tgctgttggt ttcacctacc
      301 ggagagatcc tggtggacaa gcaaatcccc tccgcgatgg cctccagtcc agttttggcg
      361 gatctcaacg gcgacggggc cctggaaata gtattcgtga cccttggcgg aatcctctgc
      421 gcggttgacc aaaacggcga ttacctgagc ggatttcctt tgaatgtcgg agcagtctgc
      481 aataccccgc ccctcattgc gaacctggac gacgatcccg ccccggaaat cctagtctcc
      541 agcaacatca actccgttta tgcctacaaa accgacggat ccatgctgga cggattcccc
      601 ttcattactt cctataacgg caccaccccg ggcaccatct gcgatttgga cagcgacgga
      661 atcttgaagc tggtgagcgg ctactccacc ggtgtcttgg tgttgaatct gcgccgcgcc
      721 gccggaggtt gcatgccttg ggtcacctat cgcggctctc taaaacgcca gggcagccac
      781 gcagctgcaa ttcttccgag ttctgacccc actgtaccgg cgctttcgga caaactgctg
      841 cagaactatc ccaatccctt caatcccagc accaccatcg ctttccagac tgctcagaat
      901 ggtgaggcgc gcctgacagt gttcaacctg aaaggccagt tcgtgcgcga actcaactcc
      961 ggatatctgc ccaaaggcga tcatagcgtg ctttgggacg gacgagacga ttgcggcaac
     1021 gctgtggcca gcggtttgta cttctaccgg ttgcaaaccc gtgaagcctc gatcacaaag
     1081 cgtatgctac taatgaagtg aaaaagggct tgacttgaaa acagccataa ccattgtaag
     1141 attatataat aaatacaaac aaggagacca aaaatgccca aagtactgat cgccacggaa
     1201 aaaccctttg ccgcggccgc cgtgcaaaaa atcaaagccg agcttgacgc cgcaggctat
     1261 gcccatgcct ggctggaaaa ctacaccgat cccaaagacc ttcacaccgc cgtcgcggat
     1321 accgatgccc tcatcatccg cagcgacaag gttgacgaag ccgtcttcgc agcagctccg
     1381 aaactcaaaa tcgtggtgcg cgccggcgcc ggatatgaca acatcgacct cgccgctgcc
     1441 acagcccaca acgtggtggc catgaacact cctggccaga attccaacgc agtggcagag
     1501 cttgccctgg ctatgatgat ctatatggcg cggggcaaat tcaacggcaa aagcggatcc
     1561 gaactggccg gcaagaagct ggtgctctac ggtttcggtt tcgttccccg ttttctggcc
     1621 aagatggcca aaggcgtagg aatggaagtt ttcgcctacg atccctacat cagccccgaa
     1681 ataatggcca aggaaggcgt caccacccta aacaccgtac aggaaatctt cagtaccggc
     1741 gactacattt ccctgcacat tcccgccaca gccgaaacca agaaatccat caactgggac
     1801 ctgctttccc tgatgaagga caattgtatc atggtgaaca ccgcgcgcaa ggaagttatc
     1861 gacgaagatt ctctgctgaa ggcctttgaa gaaaaagcga aattccgcta tgtgagcgac
     1921 gtggaacccg attgctccgc caaactggct gagctctacc ccgaccgcag ctactggacc
     1981 cccaagaaaa tgggagccca gaccggtgaa gccaacgtca atgccggcgt ggccgctgct
     2041 aagcagatca tcgccttcct cgaaaagggc gatacaacct tcaaggtaaa ttaattacgc
     2101 ctccggacct cagttcggaa cgcaaaagat aacccccggt tttttgccgg gggtttttca
     2161 atatgggtga cagcgccgct tatgcttcgc tgctgatggg gatcacagag acgaattttc
     2221 tgttgctggc cccgcgggtc tcaaatttta catggccttc gatgaggctg aacagggtat
     2281 agtctctgcc catgccgacg ttggcgccgg gatggatttt cgtgcccttc tggcgcacga
     2341 tgatgttgcc ggccacaaca tactcactgc cgtacttttt cacgccgcgg tatttgggat
     2401 tgctgtcccg tccgttgcgg ctgcttccga ctcctttctt atgtgccatt tctcatctcc
     2461 tcaaatccgg ataccggtga ctttaatttc tgtgtattgc tcacggtaac cttttttaac
     2521 acggtaacct ttcctgcgtt tggcatggta gatcaccttc tttttgcctt taccgtgctc
     2581 gattacttct gcaagtactg ctgcgccttc tactacgggc tggccgacca tgatctcgtc
     2641 ctcattgcgg acaagcaaca cccggtcaaa ctccaggctc tggcccggct ccgccgtatt
     2701 cagcagggga acgcgcagga tggcgtttgc ttcagccctg tactgaaatc ctttgatatc
     2761 aacgatggcg tacatcttga ctccttattt ttgtatggaa aatcacaatt tttcgagacc
     2821 cgctatccgt caaggggaaa attagctggt gcttaacccc ctccatttca tccccctgtt
     2881 gcctcccgca taattcccgt attcggtgcg gccattatgc gggaggcgat gtagaggcgg
     2941 aagttcgggg gctaaggttg gagtaaggtt catgtgccca aacataatat acatcaggct
     3001 tacagcgtta gccccggcag cggcgcctgt tttttgcttg aagattggaa cgattcatgc
     3061 aacccttaca atttgcctta aatacttgca agcaacacgg gaggaaaaat gcgtaaagca
     3121 atgttactgt tgacgttggc cttggggctt ggagccctga cggcgcagat gagccccctc
     3181 catctggtgg gggagggtca ttttgccggt aaggccgctg tacattccga gctgggactg
     3241 accctgggga ccattaacga ttcgacgggc gaacgctatt tcctgaaacc acaactgaaa
     3301 cgcacccact gcaaagcttt cacccgctct gagaaccgcg ccctctacag cgtgaacgac
     3361 ctgctgctct acgctgaggg cgacaatgtg gcagactggt ccgcttccac aaaaggccag
     3421 ggcctgatcc gcagcctaga tctggcttca gacggctctg ccttcctgct tcaggaaacc
     3481 gcgggaccag atacacgcca actgaaggtc cgagtccttg aggcgggtac cggtgatttg
     3541 gactatgaga tagcctacca ggtggatact ggcctcaatg ccacgactgt tttgcccttg
     3601 ggacacagaa tctttgccct gtatgaagtc ggcactctca aggtttatca ggtctattcc
     3661 ggcacgtcca ccttgcttgc cagttggccc ctgaacatca gcgacccggg ccagtatgac
     3721 ctgtccaatt ccaccctggc tcttgtgcct gccgacgatg atgttttgga tatctatctg
     3781 tggctgcgtg ctatcgggac agaaccgaac cagtccggcc aggttttaca cgccagaact
     3841 atcctgaccg ttcccaacga cgatcccggt cttcctccgg tgcttcagcc aagtctgaca
     3901 gcctggccca atccagcctt tggcaacgtc aacatcaagc tggatcacgc ttctggcagc
     3961 cagcccgttc tggaggttta caaccttcgc ggccagaagg tccgcgtgct gcagccagag
     4021 aaaacggcct ccggcagcag cttcgagtgc atctggaacg gcttggacga cagcggtcag
     4081 ccagccgcca aaggtgtcta tttcttgcgg gcaactctgc ctggcctgaa tattcccatg
     4141 aaaaggattt gcctttactg atcaatgact tactctttcc ttggg
//
LOCUS       DAOOWR010000133         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21455_length_4161_cov_3.519971, whole genome shotgun sequence.
ACCESSION   DAOOWR010000133 DAOOWR010000000
VERSION     DAOOWR010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21455_length_4161_cov_3.519971"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3241
                     /locus_tag="PLB85_04240"
     CDS             <1..3241
                     /locus_tag="PLB85_04240"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72571.1"
                     /translation="PFLISFDRNIPAFHNCRFTLELTDSAANSYQVLFYLGGFNADLS
                     VINHSVSAGGDNVLDPSENGILTLAVKNSSIFGASDIYGELTALNDLVEVTDNLSYFG
                     SIPAGMNASSIEGYGLFARPLLIPGMMIPFRLRLYNADGFEQIAHFSLPIGTVAQNTP
                     LGPDEYGYFIYDVSDTAYPDCPSYEWVEIVPSLGGSGTQITGLHDPGVSDDEGDQNDS
                     DVLETIDLPFTFRFYGVDYDQITVCVNGFIAFGVTNDGEFRNGRMPGGQGPSPMVAPF
                     WDDLVILSGGGIYQWYDSNEHRFIIQYNNLKNGYNRTSEETFQVIFYDPLYYPSSLGD
                     GIIKIQYKVFNNVDIGSSGYSPTHGKYATVGIRDHTNLRGLEYTFANQYPPAAQPLDN
                     GKALLITTVPVLHQNAYLVVGELILNDANGNSWLEPGETAEIGLKLNNLGINAATNVN
                     ISVSSQSPFLTIANPQSACPDIPGSGHAFNVVPFTVTASPDCTDGLVIPLQFVVSIDG
                     NEWTYPLNLTIRKPAIVVNDIYISDLQGNVNGLADPGETFNLIVNYLNNGFVDAYNLT
                     SNIMCLSEDVTLVNSEQLIPTIPAGAMSQAVYEVSLSPNVVVGNNLTFYLTYLGEAVD
                     AQNDTVMLNVGTTGMSEDFEEDNGAFEAQPATNGWEWGTDSTAGAHSGTKVWGTRINQ
                     QYSNNANWTLTSPPVFIGSNFVLEFYHKFDTEQNYDGGNVKISTNNGVTWTLLTPENG
                     YTHQNLSALNGPGYSGNSGGWTQARFSLAAYPNQTVRFRWTFASDGSVQRQGWYLDDV
                     QTTGFIPFAGKVSGTVQTTRPDDDYSTLFVQNDQAISIRPNSAGAYTLYLPEGEHNLT
                     ASGPGYQSQSVFPVNLSVSDPSFTQDYLLTWFAPATDLEYSVANDSLSLFWAAPAAPP
                     SPQLSYKVFRKQHSGPFELVAIVTDVAWGEIPAINGEYRYYVLACYDAGESVATNIIS
                     VVWPPVDADDPHTPPLVSCLYQNYPNPFNPSTTVMFDLAEATSVTLSVYNVKGQLVRR
                     LLQAPLGAGTHRILWDGTDESRRPVSSGIYFFRIESPALTASRKAVLLK"
     gene            complement(3642..>4161)
                     /locus_tag="PLB85_04245"
     CDS             complement(3642..>4161)
                     /locus_tag="PLB85_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72572.1"
                     /translation="LPGQPSGHVFDALYSDSTSNWVIVELNAAALDEHLPLPSVEADI
                     RDFLAHDKREQLALQISQRIISGQDPVPANSTIRNIEGLSPASQALGKHTSVIFHGIL
                     RRHQQNLLQEAFIMDGFVWLPRVISVSRDASLKVDEAQVQAVFAASLPNNWFDIWMEQ
                     QLRKANIQKYMD"
BASE COUNT          890 a         1312 c         1112 g          847 t
ORIGIN      
        1 ccccttccta atcagctttg accggaacat ccccgctttc cacaattgcc gcttcaccct
       61 ggaactcacc gacagcgccg ccaacagcta tcaggtgtta ttctacttgg gcggcttcaa
      121 tgccgacctc agcgtgatca accacagcgt gagcgccggc ggcgacaacg tgctggaccc
      181 ctccgaaaac ggcatcctca ccctcgcggt gaaaaactcc tccatctttg gcgccagcga
      241 catttacggt gaactcaccg ccctcaacga cctggtggag gtaacggaca acctctccta
      301 tttcggcagc atcccggcag gcatgaatgc ctcttccatc gaaggctatg gcctcttcgc
      361 ccggcccctg ctgatacccg ggatgatgat ccccttcaga ctcaggctct acaatgcaga
      421 cggattcgag cagatcgccc acttttcact gcccatcggc acagtggcgc aaaacacgcc
      481 cctgggtccc gacgaatacg gctatttcat ctacgacgtc tcggacaccg cctatccgga
      541 ctgccccagc tacgaatggg ttgagatagt gcccagcctc ggaggcagcg gcacccaaat
      601 caccgggctc cacgaccctg gcgtctctga cgacgaaggt gaccagaatg acagcgatgt
      661 cctcgagacc atcgacctgc ctttcacttt ccgtttctac ggagtggact atgaccaaat
      721 cacggtctgc gttaacggtt tcatcgcctt tggcgtaacc aacgacggcg aattccgcaa
      781 tggcaggatg cctggcggac agggcccctc gccgatggtc gcgcctttct gggacgacct
      841 cgtgatcctc tccggaggcg gaatctacca gtggtacgat tccaacgaac acaggttcat
      901 catccagtac aacaacctca aaaacggcta caaccgcacc agcgaggaaa ccttccaggt
      961 gatcttctac gatccgcttt actatccctc cagcctgggc gacggcatca tcaagatcca
     1021 gtacaaggtc ttcaacaacg ttgatatcgg cagcagcggc tatagcccca cccacggcaa
     1081 atatgccacg gtgggcatcc gcgaccacac caacctccgc ggcctggaat acacttttgc
     1141 caaccagtat ccgcctgccg cgcagccgct tgacaacgga aaggccctgc tcatcaccac
     1201 cgtgcccgtg ctgcatcaaa acgcctatct ggtggtgggg gaactcatcc ttaacgacgc
     1261 caacggcaat tcctggctgg aaccgggcga aaccgccgag atcgggctca agctgaacaa
     1321 cctcggcatc aacgccgcca ccaacgtaaa tatttccgtt tccagccaga gcccgttcct
     1381 caccatcgcc aatccccaaa gcgcctgtcc cgacatcccc ggcagcggcc acgcctttaa
     1441 tgtggtgcct ttcacggtca ccgcctcgcc cgactgtact gatggcctcg tgattccact
     1501 ccagtttgtg gttagcatcg acggcaacga gtggacttat cccctcaacc tcaccatcag
     1561 gaagccggcc attgtggtca acgacattta tatcagcgat ctgcagggca atgtgaacgg
     1621 actggccgat cccggcgaaa ccttcaacct gatcgtgaac tatctgaaca acggatttgt
     1681 ggacgcctac aacctcacca gcaacatcat gtgcctcagt gaagacgtca ccctggtgaa
     1741 ttctgagcag ctcatcccca ccatccccgc cggcgccatg agccaggccg tttacgaggt
     1801 aagcctttcg cccaatgtgg tcgtgggcaa caacctcact ttctacctca cctatctggg
     1861 cgaagcggtg gatgcgcaga acgatactgt catgctcaat gtgggaacca caggcatgtc
     1921 cgaggatttt gaagaagaca acggcgcatt tgaagcccag cccgccacca acggctggga
     1981 gtggggcacg gattcgactg ccggagccca ctccggcacc aaggtatggg gcacccgcat
     2041 caaccagcag tattcaaaca acgcaaattg gaccctcact tcgccgcccg tcttcatcgg
     2101 cagcaatttc gtgctggagt tttaccacaa gtttgacacc gagcagaact acgacggcgg
     2161 caacgtgaag atttccacca acaacggcgt gacctggacc ctgctcactc cggagaacgg
     2221 atacacccac caaaacctgt ccgcactcaa cggtccggga tattccggca acagcggcgg
     2281 ctggacccag gcccgcttct ccctggctgc ctatcccaat cagaccgtga ggttccgctg
     2341 gacctttgcc tcggacggct cggtccagag gcagggctgg tatctggatg acgttcagac
     2401 cacaggcttc attccctttg ccggcaaggt gagcggcacg gtgcaaacca cacgcccgga
     2461 tgacgattat tccaccctct tcgtgcaaaa cgaccaggcc atcagcatcc gtccaaactc
     2521 cgcaggggcc tacaccctct atctgccgga gggcgagcac aacctgaccg cttccggccc
     2581 cggataccag tcccagagcg tgtttcccgt gaatctgagc gtgagcgacc ccagcttcac
     2641 gcaggattat ctgctcacct ggttcgcgcc ggccacggat ctggaataca gcgtggccaa
     2701 tgattcgctt tcccttttct gggcggctcc agccgctccg ccttctccac agctaagcta
     2761 caaggtcttc cgcaagcagc attccggtcc ctttgaactg gtggccatcg ttacggatgt
     2821 cgcctggggc gaaatcccgg ccattaacgg cgaatatcgc tattacgtgc tggcctgcta
     2881 cgacgctggg gaaagcgtgg ccacgaatat catcagcgtc gtctggcccc ctgtggatgc
     2941 agacgatcca cacaccccgc cgctggtctc gtgcctctac caaaactatc cgaacccctt
     3001 caaccccagc accactgtca tgttcgacct cgcggaggca acctcggtca ccctgtccgt
     3061 ttacaacgtg aagggccagc ttgtgcgccg tctgctgcag gctcccctcg gcgcaggcac
     3121 ccacaggata ctctgggacg gcaccgacga atcacgccgc cccgtatcct ccggaattta
     3181 cttcttccgc atcgaatccc ccgcgctcac agcctcgcgc aaggcggtgc tgctgaagtg
     3241 attctctcct tatagagtgg ataaggccgg agttttcgct ccggcctttt tgggttctct
     3301 ttgaaggtgg gtgggttgcg aacagcaccg aaagtgaggg gatgccctgg agtccagcga
     3361 acgcagtgag catggactac aggaagtgtt gttgctgcta tccatgctcc ttcctcgttc
     3421 gacgccagcg tgtggcttgc tgactggccg ttaaggccct atgctggatt tgtgatacgc
     3481 ctcccgcacg atacccgcat tagatgcgag aatcatacga gaggcatggg agggggataa
     3541 acgcaggggc tcagggtctg gctaaggggg aatcgcggcg ggcaaaatgt tccaccgcca
     3601 tccgggtttt attctcttgt gtctgaaact gcctgtgtct gtcagtccat gtatttctgg
     3661 atgttggcct tgcgcagttg ctgctccatc cagatgtcga accagttgtt aggaaggctg
     3721 gcggcaaaaa cggcctggac ctgggcctcg tccactttga ggctggcatc gcgggagacg
     3781 gaaatcacgc ggggcagcca gacgaagcca tccatgataa aggcttcctg gagcaggttt
     3841 tgctggtggc ggcgaaggat gccgtggaat atgacggaag tgtgtttgcc aagggcctgg
     3901 gaggcggggg agaggccttc aatgttgcgg atggtggaat tggcagggac ggggtcctgt
     3961 ccggagatta ttctttggga gatttgcagg gcgagttgtt cgcgtttgtc gtgagcaagg
     4021 aaatccctga tatccgcttc cacagagggc aggggaaggt gttcgtcgag cgcagcggcg
     4081 ttgagttcca ctataaccca attgctggtg gaatctgaat agagggcgtc gaaaacatga
     4141 ccggagggct gaccgggcaa c
//
LOCUS       DAOOWR010000134         4157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21499_length_4157_cov_3.246221, whole genome shotgun sequence.
ACCESSION   DAOOWR010000134 DAOOWR010000000
VERSION     DAOOWR010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21499_length_4157_cov_3.246221"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLB85_04250"
     CDS             complement(<1..651)
                     /locus_tag="PLB85_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU72573.1"
                     /translation="MRRSLQLFPVLIAITLALMLACGACKAKPEPVAEKPAPTSSLTI
                     FALQHIRSSGFEAVVLKDFADKNNTALKVVVFPDLVSLLDSLSVSDSLANADIVLGLD
                     SAFALSDSILKPFAPLTEISLIEVSLDIPRDPGQRLIPYATANLSFICDTRKYPDPPK
                     SFGELQDARYYNQMGLCDPSTSGLGRSSLLWTVALFGERGYDQLWNSFRKTSATPTG"
     gene            complement(654..1397)
                     /locus_tag="PLB85_04255"
     CDS             complement(654..1397)
                     /locus_tag="PLB85_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU72574.1"
                     /translation="MKLDFLAGVGAFTIFIGRVLKSLPRLGKRREEFVIQLKRIGIDS
                     LPLIALTSSFTGLVTALQLVYQGRGYIPDHLFSVLISKSTMIELAPVLTALVMTGKVG
                     AAIAAEIGSMKVSEQLDALRSMSVEPEEFLYLPRIAAGVVAFPLLTVFANMVGIGSAW
                     FFNWVQNGIHYHTFFNNIRSYFQTFDLLSGIVKSIVFGFVITSLACFFGDRTRGGAEG
                     VGRSTMQTVVYSSVWVLVMDFIVAFLILG"
     gene            complement(1387..2766)
                     /gene="dnaB"
                     /locus_tag="PLB85_04260"
     CDS             complement(1387..2766)
                     /gene="dnaB"
                     /locus_tag="PLB85_04260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU72575.1"
                     /translation="MAKKTESVPKDIIQISDRALPADVNAEAGVLSAMIIDSGIVSKG
                     IETIKEEYFSRAANKLVFRSICELLYEGVETDPVTLASRMERNNTLEKAGGIPYINDL
                     ADFVVSSANFDYHLTILTEKALLRHLIVACNGIIESCYSSPKAVKDIVDEAEQAIFKI
                     AELPHQIGFQRFDEISHEVLQTIDAIATKKIPVIGIPSGYGDLDRLTGGFRPGQFIII
                     AARPAMGKTSLALNIASHAAVNMGKKVAIFTMEMAADEVIMRMFSSASEVNMDAMLKG
                     YGMNEEKMIRIMQASEVLSAKPIYIDESGTNTPLEVRAKVRRLAAEIGGVDLIIIDYL
                     QLMTVPKDRDSRQQEISEISRALKILAKDMKIPVVALSQLNRMLETREDKRPRLADLR
                     ESGAIEQDADLVMFIYRDEYYYGEKSEKTGIAEIIVGKNRHGSTGSVDLGFVKEFTLF
                     RSIDTVHET"
     gene            complement(2759..3244)
                     /gene="coaD"
                     /locus_tag="PLB85_04265"
     CDS             complement(2759..3244)
                     /gene="coaD"
                     /locus_tag="PLB85_04265"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU72576.1"
                     /translation="MTCKAIYPGTFDPITLGHVNILGKAARLFEKVILAVADYTGKEN
                     LFSFEERIRLCTEAVKHIPNVEVLGFEGLAVEFARKQECRVMIRGLRAVSDFEYELAL
                     ALTNTKLDPGIETIFLVPSLRYMYLSSSMIRQLAELGSELNDSVPENVALALVEKHKD
                     G"
     gene            complement(3241..>4157)
                     /locus_tag="PLB85_04270"
     CDS             complement(3241..>4157)
                     /locus_tag="PLB85_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOU72577.1"
                     /translation="PTLSGMCPLIKVIHANLGFAGLNQPPTLAMHMLQPIRDRQPQIQ
                     IKQLNDLITPLRKIKDNWEVKQLQKAIDITGQGIEDIVSSAAAGMMEYELEATLFYRM
                     QVNGLQAWGFAPIIAAGINAATLHYEKNNCRIGDNELVLMDVGADCNGYSADVTRCFP
                     IGKKFTARQKQIYSLVLDVQKKVIAAVKPGVKLSELNDITKKGLAQGLIALNLIDSED
                     QVGRYYMHSVSHFLGLDTHDLGGREAVLEEGNVITVEPGLYLPEENLGVRIEDDVLVT
                     KTGHKVLSAKIPKEIKDIEKLRRRVTVP"
BASE COUNT          852 a         1064 c         1258 g          983 t
ORIGIN      
        1 cccggtaggg gtggcggacg ttttacggaa gctgttccat agctgatcat aaccgcgttc
       61 cccaaacagg gccacggtcc agagcaaaga agacctcccc agccccgaag tggaggggtc
      121 gcagagtccc atttggttgt agtagcgggc atcctgaagc tctccgaacg acttgggcgg
      181 gtctggatat ttgcgggtgt cgcagatgaa gctgagattg gccgtggcat aggggatgag
      241 gcgctggccg gggtcgcggg ggatatcgag gctgacctca atgagtgaaa tctcggtcag
      301 gggagcaaat ggtttcagga tcgagtcgga gagggcgaaa gcgctgtcca gccccagcac
      361 gatgtcggca ttggcaaggc tgtccgaaac gctgagggaa tccagcagcg agaccaagtc
      421 cgggaaaacg accaccttca gggcggtgtt gtttttgtcc gcgaaatcct ttaggacaac
      481 tgcctcgaag ccggaggagc ggatgtgctg gagggcaaag atagtcaggg aactggtcgg
      541 ggctggcttt tccgcaaccg gttccggttt ggctttgcag gctccgcaag ccagcatcaa
      601 tgcaagggtg atggcaatta agaccggaaa aagctggagg gaccgcctca caactacccc
      661 aggatcagga aagccacgat gaaatccatc accagcaccc acacggagct atagaccacg
      721 gtttgcatgg tgctgcgtcc cactccctcg gcgccgcctc tggttcggtc gccgaaaaaa
      781 caggccaggg aggtgatcac gaagccgaac acgatggact tcacgatgcc gctgagcagg
      841 tcgaaggttt gaaagtagct gcggatgttg ttgaaaaagg tgtggtagtg gatgccgttt
      901 tgcacccagt tgaaaaacca ggcactgccg atcccgacca tgttggcaaa gacagtgagc
      961 aggggaaagg ccaccacgcc tgccgcgatc cggggaaggt aaagaaattc ctccggttcc
     1021 acgctcatgc tgcgcagggc gtccagttgt tcgctaacct tcatggagcc gatctccgcg
     1081 gcaattgcgg cgcccacttt accggtcatc accaaagccg tgagcaccgg cgccagctca
     1141 atcatggtgg atttgctgat gagcacgctg aagaggtggt ccggaatgta gccgcggccc
     1201 tgatagacaa gctgcagcgc ggtcacgagg ccggtgaaag atgaggttag ggcgataagc
     1261 ggtagtgaat caatgccgat gcgcttgagc tggatgacga attcctcgcg gcgcttcccc
     1321 aaacgcggca ggcttttcag caccctgcca atgaagatgg tgaaggcgcc aactccggcg
     1381 aggaaatcaa gtttcatgca cagtgtcgat gctgcggaag agggtgaatt cctttacgaa
     1441 gcccaggtcc accgaacctg tggagccatg gcggtttttt cccacgatta tttccgctat
     1501 gccggttttt tcggattttt ctccataata atactcatcg cggtagatga acatcaccag
     1561 gtcggcgtct tgctcgatgg cgcctgactc acgcaggtcg gccaggcggg ggcgcttgtc
     1621 ctcacgggtt tccagcattc ggtttagctg ggagagggcc accacgggga ttttcatgtc
     1681 cttggccagg attttcagcg cacgggatat ttctgatatt tcctgctggc ggctgtcgcg
     1741 gtctttgggc acggtcatga gctgtaaata gtcgatgatg atgagatcaa cgccgccgat
     1801 ttcagcggca aggcgccgga cctttgcccg gacttccagg ggggtgttgg tgccggattc
     1861 atctatgtag atgggtttgg cggagagcac ttcggaagcc tgcatgatgc ggatcatctt
     1921 ttcctcgttc atgccgtaac ccttcagcat ggcgtccatg ttcacttccg aagcgctgct
     1981 gaacatcctc attatcactt cgtccgcagc catttccatg gtgaagatgg ccactttttt
     2041 gcccatgttc accgcagcgt gggaagctat attcaatgcc agggaggttt tacccatggc
     2101 ggggcgcgcg gcgatgatga tgaactggcc aggcctgaaa ccgccggtga ggcggtcaag
     2161 gtcgccgtag ccgcttggaa ttccaatcac cggaatcttc tttgtagcaa tggcgtctat
     2221 ggtctgcagc acctcgtggc tgatctcgtc aaaacgctgg aagccaattt ggtggggaag
     2281 ttcggctatc ttgaaaattg cctgctcggc ttcgtcaacg atgtctttca ccgctttggg
     2341 cgagctgtag caggattcta tgatgccgtt gcaggccact atcagatgac gcagcagggc
     2401 tttttcggtg aggatggtga gatggtagtc gaaattggcg ctggagacca caaagtcggc
     2461 cagatcgttg atatagggta ttccgccggc tttttccagg gtgttgttgc gctccatacg
     2521 gctcgccagg gtcacgggat cggtttccac gccttcgtat aatagctcgc agatggagcg
     2581 gaagaccagt ttgttcgctg cgcgggaaaa gtattcttcc ttgatagtct ctatgccttt
     2641 gctcacaatg cccgaatcta tgatcatggc ggaaagaacg ccggcttcgg cattcacgtc
     2701 tgcgggcagg gcgcggtcgg aaatctggat tatgtctttt gggacagatt ctgttttctt
     2761 agccatcttt atgcttctct acaagggcca gggccacgtt ttccggcaca gaatcattga
     2821 gttcggagcc gagttccgcc aattgccgta tcatagaaga agaaaggtac atatagcgca
     2881 ggctgggcac caggaaaatg gtttcgatgc cgggatcgag cttggtgttt gtgagggcca
     2941 aagccagttc gtattcaaag tcagaaacgg cacgcaggcc gcggatcata acccggcatt
     3001 cctgtttcct ggcgaattcc accgccagtc cttcaaaacc taacacttcc acgtttggga
     3061 tgtgtttaac agcttcagtg caaagacgga tgcgctcttc gaagctgaaa agattctctt
     3121 tgccggtgta gtccgccacg gccaggataa ctttttcaaa aagtctggcg gcttttccca
     3181 gaatgttcac gtgacccagg gtgatggggt cgaaggtgcc gggatagatg gctttgcagg
     3241 tcatggcaca gtcaccctgc ggcgcagctt ctcaatgtct tttatttctt tggggatctt
     3301 ggcggaaagc actttgtggc cggtttttgt gaccagcacg tcgtcttcga tgcgcacgcc
     3361 gaggttttcc tctggaaggt acaggcctgg ctcgacggtg atcacgttgc cctcttccaa
     3421 gactgcttca cggccgccga ggtcgtgggt gtccaaaccc aggaagtggc tcacgctgtg
     3481 catgtaatat ctgccgacct ggtcctcgga atcgatcagg ttcagggcga ttaggccttg
     3541 cgccaatccc tttttggtga tgtcgttgag ctcggaaagc ttcacgcccg gtttcacagc
     3601 cgcgatcacc tttttctgca cgtccagcac taggctgtag atctgtttct gtcgggctgt
     3661 gaatttcttg ccgataggaa agcagcgtgt aacgtctgcg ctgtagccgt tgcagtctgc
     3721 gcccacgtcc atcaacacca gttcgttgtc tccgatccgg cagttgttct tttcgtagtg
     3781 gagagtggcg gcgttgatgc ccgcggctat gatgggggcg aacccccagg cttgcaggcc
     3841 gttcacctgc atgcggtaaa agagggttgc ctccagctcg tattccatca tgcctgccgc
     3901 ggcggaactt acgatgtctt caatgccttg gccggtgatg tcgatggcct tttgcagctg
     3961 cttcacttcc cagttgtctt tgatcttgcg cagaggggtg atgagatcgt tcaattgttt
     4021 aatctggatc tgtggctggc ggtcacgaat gggctggagc atgtgcatgg ccagggtggg
     4081 cggctggttc aggccggcga agccaaggtt ggcatggatc actttgatga gggggcacat
     4141 gcctgacagg gtggggt
//
LOCUS       DAOOWR010000135         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21532_length_4153_cov_4.324061, whole genome shotgun sequence.
ACCESSION   DAOOWR010000135 DAOOWR010000000
VERSION     DAOOWR010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21532_length_4153_cov_4.324061"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..986)
                     /locus_tag="PLB85_04275"
     CDS             complement(30..986)
                     /locus_tag="PLB85_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOU72578.1"
                     /translation="MKKNKLVLLAVFLLMLASVVILVLTRRDRESSRAIFSADSTAVA
                     AIEIASPDTSISFALENGRWRITKPVAWGVEEGHFQLFMRDVILKQYSTEPIASGKEA
                     LKQYRLTKDKALRIKAFDARNKLLREVWFGDLGNPFDYFCFAGGSNVFQIRSKVNSFY
                     GPKLESWRSPYALSLFSDQLLTINVKHQKNSYELTRNGDIWHYSDKIEDFDIPPGNLV
                     MGKLLNALSHLGSHTMLSGETLPPASSVPAPECEVDLMLNDNSRVKLSFHAWEDKNYL
                     LKIDRYPDSYFVVLFDTVFRFTRHAALFRAVVGDPSSIEPQN"
     gene            complement(983..2548)
                     /locus_tag="PLB85_04280"
     CDS             complement(983..2548)
                     /locus_tag="PLB85_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOU72579.1"
                     /translation="MNTRSQIFGTYAVKILIAVLALILASFLYLRLDASSRRAHSLSK
                     ATKASLRSLQEKVVVKVFASNDLPQQLSSLNRDLKDMLEEFQRNSRGKLKYEYVRAKS
                     TEELIDEARQYNIPPLNATIYEEDKMVSKEIVFGVSFEGGGKSSSMYLRPGMETMLEY
                     QLLKEVNKLQPETLPKVAFFADSLALMYQYAYYQDNVATFFLELTENYNLEVTRLDSL
                     PQAPVMLCMGVVDSLSTLQLYHLDQYLMRGGEVVMLQDRALVTYSSQGTGVSEVNSNV
                     FRLLGHYGIFIKPNIVLDEECELGRGSGLGTQVPYPFFPRLKPNPDVPYALGFDPIVM
                     NIASELVTMPGSKLKRLPVLTTSGKSNSLIGPYFNIEEAINRSQEPGWLSMPPLTVAA
                     EFSGPLKSFFSQALTDSTFHPSNPRGRVILFGDSEFNLEFGSGAYMVQNAIDHLLGRA
                     EMVKLRSQKTVYNRLGVDVYMGRNQMRPAEPAKTISNLTLGFKLTAILLPLLLLAFVG
                     FAQAYRRSNRGTL"
     gene            complement(2550..3272)
                     /locus_tag="PLB85_04285"
     CDS             complement(2550..3272)
                     /locus_tag="PLB85_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU72580.1"
                     /translation="MNPALTIAKKEYSLALRSLGTYIIFGVFLLAAGIWFALTALKLG
                     VADMRETLARMHSLFLFFIPAITMGSIAKERSSGTLELISTLPIRLGHIVWGKFLAAW
                     AQLATVLAFSLVFAALIFIFGVGVDAGAIFTGYLGLLCAGAAYIAIGIFASSLTENQV
                     LAFIIALAISGVLFLLGRLGELIPLKLFAALEYFGFDYHLNSFIKGVIDTRDLIWFAA
                     VTFIFMLLAQFRLQSANLMQER"
     gene            complement(3269..>4153)
                     /locus_tag="PLB85_04290"
     CDS             complement(3269..>4153)
                     /locus_tag="PLB85_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU72581.1"
                     /translation="DISTDPVAVKKRIGYLPEHNPLYPEMSVGEFLIYLGHLRRMKEA
                     KLGERLEFVREACGLDEVWNQRIATLSKGYRQRTGLAGAILHDPEVLILDEPTGGLDP
                     NQIMEIRELIRDLGQAKTVLLSSHIMQEVQALCSRVIIINKGRVIVDDRIENLGAHIS
                     GFQRVILELEAVEPDFEPWLSQYPEVKLDSQSQNGEACLLHFLAPPETDIRRELSAFV
                     AAQSWHLLSIYQEKQSLEHIFHELTSQEKIPVEEEPQLPQEEIGKLVARHDPIDSDLI
                     CPGEDLPPENGNNNKEQQ"
BASE COUNT          846 a         1108 c         1306 g          893 t
ORIGIN      
        1 agaggtcggg atcgagttcc cggcagcggc taattttgag gttcaatcga gctggggtcg
       61 cccacaactg cccggaacag cgcggcgtgg cgagtgaagc ggaacacggt gtcgaaaagc
      121 accacgaaat agctgtccgg atagcggtct attttgagca ggtagttttt gtcctcccag
      181 gcatggaagc tgagcttcac ccgggaattg tcgttcagca tcaggtccac ctcacattcc
      241 ggggcgggga cggaagaggc cggaggcagg gtctcgccgc taagcatggt gtgcgaaccc
      301 agatgggaaa gggcattcag cagtttgccc atcaccaggt tgcccggcgg aatgtcgaaa
      361 tcctcaatct tgtcgctgta gtgccagata tcgccgttgc gggtcagctc gtagctgttt
      421 ttttggtgct ttacattgat ggtgagcaac tggtcggaaa agaggctgag cgcatagggc
      481 gagcgccagg attccagctt ggggccatag aaggagttca ccttgctccg gatctggaaa
      541 acgttggacc cgccggcgaa acagaagtaa tcgaagggat tgcccaaatc gccgaaccag
      601 acctcgcgca gcagcttgtt tcgggcgtcg aacgccttga ttcgcagggc cttgtccttc
      661 gtgagtcgat actgtttgag ggcctctttg ccggaggcga tgggctctgt ggaatattgc
      721 ttcaggatca catcccgcat gaaaagctgg aaatggcctt cctccacgcc ccacgcgacg
      781 ggtttggtaa tgcgccatct gccgttttcc agggcgaagg agatgctggt gtccgggctg
      841 gcgatttcga tggcggccac cgccgtgctg tcggcgctga agattgccct gctgctttcc
      901 cggtcgcggc gggtgagcac tagtatgacc accgacgcca gcatcaacag gaaaacagcc
      961 agcagaacca gcttgttctt tttcacagcg tcccccggtt cgagcgcctg taagcctgcg
     1021 cgaagcccac aaaggccaga agcagcagag gcagcaggat agcggtgagc ttgaagccca
     1081 aggtgaggtt ggatatcgtt ttggcaggtt cggcggggcg catctggttc ctgcccatgt
     1141 aaacgtccac gcccaggcgg ttatagaccg tcttttgcga gcgcagcttc accatttcgg
     1201 cgcgtcccag caggtggtca atggcgtttt gcaccatgta ggcgccggag ccgaattcca
     1261 ggttgaactc actgtctccg aagaggatga cgcggcccct ggggttggag gggtggaagg
     1321 tgctgtccgt aagtgcttgg gagaaaaagc ttttgagcgg gccgctgaat tccgctgcta
     1381 cggtcaatgg cggcatgcta agccagccgg gttcctggct gcggttgata gcttcctcga
     1441 tgttgaaata aggccctatc agggaatttg actttccgga ggtcgtcaaa acaggcaagc
     1501 gcttcagctt cgagccaggc atcgtgacca gttccgaggc tatgttcatc acgatgggat
     1561 cgaaaccgag ggcgtaaggc acatcgggat ttggtttcag ccgggggaag aagggatagg
     1621 gcacctgggt gccgaggccg gagcccctgc caagctcgca ttcctcatcc agaacgatgt
     1681 ttggcttgat gaaaatcccg tagtgtccca gcagccggaa gacgttgctg ttcacctccg
     1741 aaacgcccgt gccctgggag ctgtaggtca ccaaagccct gtcctgaagc atcaccacct
     1801 ccccgccccg catcaggtat tggtccagat ggtagagctg aagggtggaa agcgaatcca
     1861 ccacgcccat gcagagcatc acaggggcct ggggcaggct gtccagcctg gtgacttcga
     1921 ggttgtagtt ctccgtcagt tccaggaaaa aggtcgccac gttgtcctga taataggcat
     1981 actgatacat cagggccagc gaatcggcga agaaagcaac cttgggcagc gtttccggct
     2041 gaagtttgtt aacctctttg aggagttggt attccagcat tgtctccatg cccgggcgca
     2101 gatacatcga tgaggatttg cccccgcctt caaagctcac accgaagacg atttccttgc
     2161 tcaccatctt gtcttcctca tagatggtgg cattcagggg cgggatgttg tattgccggg
     2221 cctcgtcgat aagctcttcg gtgcttttgg cccgtacata ctcatatttt agcttgccgc
     2281 gggaattgcg ctgaaactct tcaagcatgt ccttcaggtc gcggttcagg ctgctcagtt
     2341 gctggggcag gtcgttagag gcgaagactt taaccaccac cttttcctgc agggagcgca
     2401 ggctggcttt ggtggccttg ctgagggaat gggcgcggcg gctgctggca tccaggcgca
     2461 aatagaggaa gctggccaga atgagcgcga ggacggcgat gaggattttg acggcgtagg
     2521 ttccgaaaat ttggctgcgg gtgttcatgt tatctctcct gcatcaggtt tgcgctctgg
     2581 aggcggaatt gggccagcag cataaagatg aaggtgaccg cggcaaacca gatgaggtcg
     2641 cgggtgtcga tcacgccttt gatgaagcta ttcagatggt agtcgaagcc gaaatactcg
     2701 agggcggcaa agagcttcag gggaatcagt tcgcccaggc ggcccagcag gaacaggact
     2761 ccggagatgg ccagggcgat gatgaaggcc agcacctggt tttccgtgag actgctggca
     2821 aagatgccga tggcgatgta agccgccccg gcacagagca ggcctaggta gccggtgaaa
     2881 atggcgccgg catccactcc gactccgaag atgaagatca gggcggcaaa aacgaggcta
     2941 aaagccagaa cagtggccag ttgcgcccag gcagccagga attttcccca aacgatgtgc
     3001 cccagcctga tgggcagggt ggaaatcagc tccagggtcc cgctgctgcg ctctttggcg
     3061 atgctgccca tggtgatggc cgggatgaaa aagaggaaca gggagtgcat cctggccagg
     3121 gtttcacgca tgtccgccac gcccagcttg agggccgtga gggcaaacca gatgccggca
     3181 gcgaggagga aaacgccgaa gatgatgtag gtgcctaggg agcgcagggc cagcgagtat
     3241 tcctttttgg cgatggtgag ggcgggattc attgctgctc cttgttgttg ttgccgtttt
     3301 ccgggggcag gtcctccccg gggcagatga ggtcactgtc gatggggtca tgcctggcca
     3361 ccagtttgcc aatctcctcc tggggaagct ggggctcctc ctccaccggg attttctcct
     3421 gcgaggtaag ctcgtggaag atgtgttcca ggctttgctt ttcctggtag atgctcagca
     3481 agtgccagct ctgggctgcc acgaaggctg aaagctccct gcggatgtcg gtttctggcg
     3541 gggcaaggaa atgcagcaag caagcctctc cgttttggga ttgcgaatcc agcttcacct
     3601 ccgggtattg actcagccag ggttcaaaat ccggttccac cgcctcgagt tccagaatca
     3661 cgcgctggaa gccggagatg tgggcgccca ggttttcaat cctgtcgtca actatcacgc
     3721 ggcccttgtt gatgatgata acgcgcgaac acagggcctg cacttcctgc atgatgtggc
     3781 tggagagcag cacggtcttg gcctgtccca agtcgcggat gagttcgcgg atttccatga
     3841 tttggttggg gtccagtccg ccggtgggct catccaggat gagcacttct ggatcgtgga
     3901 gaattgcccc cgccagcccg gtgcgctgcc ggtagccctt ggaaagcgtg gcgatgcgct
     3961 ggttccagac ctcgtccagg ccgcaggctt cgcgaacgaa ttccagcctt tcccccagct
     4021 tcgcctcttt catccggcgc agatggccca ggtagataag gaattccccc accgacattt
     4081 ccgggtaaag gggattgtgt tccggcagat agccgatgcg ttttttcacc gccaccgggt
     4141 cggtgctgat atc
//
LOCUS       DAOOWR010000136         4130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21696_length_4130_cov_3.300613, whole genome shotgun sequence.
ACCESSION   DAOOWR010000136 DAOOWR010000000
VERSION     DAOOWR010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21696_length_4130_cov_3.300613"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLB85_04295"
     CDS             <1..674
                     /locus_tag="PLB85_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72582.1"
                     /translation="PYHQTASANNIMITPADPVHQVDFTLINLPQPANAAFDVDNLTG
                     EVVLSWEPPIDPVLPVMNYRVYKKFNTGPFELIQQTTGLGYTDVISLDGDYAYLIIAV
                     YLNIEGCPSDTLAFEFPYVVENSDENAPQLVTRLNSNYPNPFNPSTTISFDLARPGKA
                     RLSVYNVKGQLVKELVDGDMASGQHRVIWNGLDSSGRSVSSGVYFYRLEAGDYVSTRK
                     MLMIK"
     gene            1057..2724
                     /locus_tag="PLB85_04300"
     CDS             1057..2724
                     /locus_tag="PLB85_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72583.1"
                     /translation="MISIFIDYKLARYQHEIKYAFSFIFQTLGYGYCFISDTGQLKDN
                     DILFIYGYTDPTVEELKGIARHFITIFIQSEPDLFDPKNMNPEKLRRSLKTIKLLSQT
                     PVISARSFSYPAENYSESEVHAGKINFDLVGNLFFHLAGLEAQIDPTRDADGCFPDEA
                     SQFNPHRDTPYVDNLLWLIDSMIKEHTRAKGIHIVQKQYWPQAQEGAALLSHSVDDLQ
                     KWNYSELFLSVGSDLAMLFSLSWRQLLHSIGSKFRFLFTNVELYWNFAEYRQLEEDAG
                     FRSVFYIAPEKNEYINYNLDDADLQEEIQQLTRAGHDVGLLLAPDKPTRDEFVSRKQI
                     MLHQLRKDQIGIRQLHYRSSDVMRDLHNKLGPSHSQCTARRDTPGFVSGISVPYQPWI
                     GGLKTNWWELPTVYHDAHLRVGRYKTLQLEQAKHLLKKYFQAALRTRGVFGLDLSLAS
                     YADIPYVKKLYAYALALLKAGRVWVPTPAELTAWWDKRNRVTIEESDYEISIYFPDEM
                     EHFALQVLNEDKIREVDGLPARVEGNMVYFTGVPAEAIAVIRLNREP"
     gene            2721..3854
                     /locus_tag="PLB85_04305"
     CDS             2721..3854
                     /locus_tag="PLB85_04305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72584.1"
                     /translation="MTKLLHLQLLPLLSGVQNFSLHLLDGLPRDEFEIYVASAPGGDL
                     VDAVRQRGYKHIPLRFLRHPISPLDATALAELLLMLGRHRFDIVHTNASKPGLLGRLA
                     ARLCHVPLILHTEHGTAFQEDQPAFQQYFFRQLEKVGNSLGHKVVFVNHSDRENCLSL
                     GLLPEEKALTINNALPPVQAEKLAQVAADRKFDPDKQDFVIGSTFRFTTQKNVVNITG
                     AACQACAREPGLRFILLGDGEYLQLCRQIVSSHGLNSRVLLPGWEADIGKWLPLFDAF
                     LLYSRWEAQPFSIIEAMHAGLPILGSDIPSIRELVGPDCGWLVPLDDQTALINCLVEV
                     SRDRADAFQKGRTAAQTISGICSYEKMVAGYLKLYREGAGVRK"
     gene            3857..>4130
                     /locus_tag="PLB85_04310"
     CDS             3857..>4130
                     /locus_tag="PLB85_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HOU72585.1"
                     /translation="MLIIFLSLGIFLLTHLLVPLNIRLSKRFGILALPNERKIHTQAI
                     PEAGGLSFALPMMLAQLVLGLTLLPDLSGRMMLQLAGVEAIALILGV"
BASE COUNT          919 a         1273 c         1065 g          873 t
ORIGIN      
        1 tgccttacca ccaaaccgcg agcgccaaca acattatgat cactcccgct gatccggtgc
       61 accaagtcga tttcaccctc atcaacctgc cccagccggc caatgccgcc ttcgacgttg
      121 acaacctcac cggtgaagtt gtgctaagct gggaaccgcc catagacccc gttctgccgg
      181 tgatgaatta ccgcgtgtac aaaaagttca acaccggtcc tttcgagctg atccagcaaa
      241 ccaccgggct tggctacacc gacgtgatct cgttggatgg agattacgcg tatctgatca
      301 tcgcggttta cctcaacatt gaaggctgcc cctcagacac ccttgccttc gagtttccct
      361 atgtggtgga aaacagcgac gaaaacgcgc cgcaactggt tacgcgcctc aattccaact
      421 accccaatcc cttcaatccc agcaccacca tctccttcga cctcgcgcgg ccgggcaaag
      481 ccagactcag cgtgtacaac gtcaaaggcc agctggtgaa ggaacttgtg gacggagaca
      541 tggcctccgg acagcaccgg gtcatctgga acggactgga ttcctccggc cgcagcgttt
      601 cctcaggcgt ttatttctac cggcttgagg ctggagacta tgtttccacc cgcaagatgc
      661 tgatgatcaa ataagcaaac gttatcccgc ctttgccaat cgtggcggcg gacaagaatc
      721 aaaaccccgg caggtgccgg ggtttttctt aggggattat tgttcgcctg ccccaaacct
      781 ccctcaagat ggccgcatct catgcgggga tcatgcggga ggcagaccag gaacttggta
      841 tcgggggcta acgaggaatg cggttccttc cttatttgct gtctttgcct ttcaacctgc
      901 gtcctgattc cacaaatcag cagcttcaag tatgtggcca aagcgcacaa aactgaggca
      961 agttcatgcg aaaaaagtgg caacggacga ttttgcttga cacatagtaa aaaaaggaat
     1021 ctctggcttt aaaaagaaat tggcggtaag ctgcgcatga tctcgatctt catagattac
     1081 aagctggcga ggtatcagca cgagataaag tacgccttca gcttcatatt tcagaccctg
     1141 ggatacggat attgcttcat ttcggacacg ggccagctga aggacaacga catcctcttc
     1201 atctacggtt acaccgatcc tacggtggaa gaactgaagg ggatagcgcg ccatttcatc
     1261 accatcttca tccagagcga gcccgacctc ttcgatccca aaaacatgaa tccggaaaaa
     1321 ctccgccgca gcctgaaaac catcaagctg ctctcgcaga ccccggtgat cagtgcccgc
     1381 agcttttcct atcccgctga aaactattcg gaaagcgaag tccacgccgg caaaatcaat
     1441 ttcgaccttg tgggcaacct tttcttccat ctggccggcc tggaagcgca gatcgatccc
     1501 acccgagacg cggatggctg ctttccggat gaggccagcc agttcaatcc ccatcgcgac
     1561 acaccctacg tggacaacct gctctggctg atcgacagca tgatcaagga acacacccgc
     1621 gccaagggca tccacattgt tcaaaagcaa tactggccgc aagcccagga aggcgcggcc
     1681 ctgctttcgc acagcgttga cgacctgcag aaatggaact actcggaatt gttcctctcc
     1741 gtgggcagcg acctcgcgat gcttttttcc ctcagttggc gccagctttt gcacagcatc
     1801 ggcagcaaat tccgctttct cttcaccaat gtggaactct actggaattt cgcggaatac
     1861 cgccagttgg aggaggatgc cgggttccgc agcgtattct acatagcccc ggaaaagaac
     1921 gaatacatca attacaacct ggatgacgcc gacctccagg aagagattca gcagcttacc
     1981 cgcgcagggc atgacgtcgg cttgctgctt gctcctgaca aacccacccg cgacgagttc
     2041 gtttcccgca agcagatcat gctgcaccaa ctgcgcaagg accagatcgg catccgccag
     2101 ctccattacc gctccagcga cgtcatgcgc gatctgcaca acaaactggg cccctcccac
     2161 agccaatgca ctgcccgcag ggacactcct ggcttcgtga gcgggatcag cgttccttac
     2221 cagccctgga tcggcggcct caaaaccaac tggtgggagc ttcccaccgt ctatcacgac
     2281 gcacatctgc gcgtggggcg ctacaaaacc ctgcagctcg agcaggccaa gcatctgctt
     2341 aaaaagtatt ttcaggccgc cctccgcacc cgcggcgttt ttggcctcga cctgagcctc
     2401 gcctcctatg ccgacatccc ctatgtgaaa aagctttacg cctacgcgct ggcactactc
     2461 aaggccggcc gagtttgggt cccgactccg gcagaactca ccgcctggtg ggacaaacgc
     2521 aacagggtga ccatcgaaga gagcgattat gaaatcagca tctatttccc ggatgaaatg
     2581 gaacattttg ccctgcaggt gctgaacgag gacaaaatcc gcgaagtgga tggcctgccg
     2641 gcccgggtgg aaggaaacat ggtttacttc accggcgttc cggctgaggc gatcgcggtt
     2701 attaggctga accgggaacc gtgaccaaac tgctccatct gcaattgctg ccgctgctct
     2761 caggtgttca gaatttcagc ctgcaccttc tggatggctt gccacgtgac gaatttgaga
     2821 tttacgtggc aagcgccccc ggcggggatc tggtcgatgc cgtgcgccaa aggggctaca
     2881 aacacattcc gttgcgcttc ctgcggcatc ccatctcgcc tctggacgcc accgccctgg
     2941 cggaattgct gcttatgctg ggtcgccacc gcttcgacat cgtgcacacc aatgcctcca
     3001 aaccaggtct gctgggacgt ttggcggcca ggctctgcca tgtcccgcta atcctgcaca
     3061 ccgaacacgg cactgccttt caggaagacc aacccgcctt ccagcaatac tttttccggc
     3121 aactggaaaa agtgggtaat tcgctggggc acaaagtggt tttcgtgaat cactccgacc
     3181 gcgaaaactg cctgtcgctg ggacttttgc ctgaagagaa ggcccttaca atcaacaacg
     3241 ccctgccacc cgtccaggcg gaaaaactgg cccaggttgc cgccgaccgg aagtttgatc
     3301 ccgacaaaca ggatttcgtg atcggctcca ccttccgttt caccacccag aagaacgtgg
     3361 tgaacatcac cggggccgct tgccaggcct gcgcccggga acccggtctg cgcttcatcc
     3421 tgctgggcga tggcgaatac ctgcagcttt gccgccagat agtgagctcg cacggtctca
     3481 attcgcgggt tctgcttccc ggctgggaag cggacatcgg caaatggcta ccccttttcg
     3541 atgccttcct tctctattcc agatgggagg cacagccttt cagcatcatc gaagccatgc
     3601 acgcgggcct gcccatcctg ggttcggaca ttccctccat ccgcgaattg gttggcccgg
     3661 attgcggctg gctggtcccg ctggatgacc agaccgcttt gatcaactgc cttgtggaag
     3721 tttcccgcga ccgcgccgat gccttccaga aagggcgaac ggcggcccaa accatcagcg
     3781 ggatatgctc ttacgaaaag atggtggccg gctatctgaa gctttaccgg gaaggcgcgg
     3841 gggtcaggaa atgacgttgc tcatcatctt cctcagcctg gggatttttc tgctcacgca
     3901 tctgctggtg ccgctaaaca tcaggctctc caagcgtttc ggcatccttg ccctgcccaa
     3961 cgaacgcaag atccacaccc aggccatccc cgaggcaggg gggctgtctt ttgccctgcc
     4021 catgatgttg gcgcagttgg tgttgggatt gaccttgctg ccggacctct caggcaggat
     4081 gatgctgcaa ctggctgggg tggaggcgat agcgctgatt ctgggcgtta
//
LOCUS       DAOOWR010000137         4110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21821_length_4110_cov_4.158076, whole genome shotgun sequence.
ACCESSION   DAOOWR010000137 DAOOWR010000000
VERSION     DAOOWR010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21821_length_4110_cov_4.158076"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /gene="nifJ"
                     /locus_tag="PLB85_04315"
     CDS             <1..2210
                     /gene="nifJ"
                     /locus_tag="PLB85_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU72586.1"
                     /translation="IIGDNTDLYVQGYFQYDSKKSGGITRSHLRFGKKPIQSQYFVTK
                     ENFVACHNQAFIGRFDLLGGIKENGVFLLNSNWEREEAFNNLTSDMQQIIIDRKIKFY
                     NIDGLKIAEAVGLGGRVNTVMQTAFFLISGILERGEAIQLIKESVRKTYGRKGEEVVQ
                     MNLKAIDHVNEALKEVEIPATLPDQCAPRKKLVPDGSDAFVTGVIEPIMREEGDRIKV
                     SQMPIDGYVPSGTAKLEKRRVAPTVPHWIAENCIQCNQCSFVCPHAAIRAKLVKDEDL
                     GNAPASFNTLKATGAEGYQYKVQVYIDDCQSCRVCVNECPKAALEMRPIEDERDAGEQ
                     ANYEYFEALPSDVLATFKETNVKGSQFKQPLLEFSGACAGCGETPYIKLLTQLYGDRM
                     IIANATGCSSIWGGTFPTIPYAVNKDGKGPAWANSLFEDNAEYGFGMRLAVNSHRKLL
                     RHYMEQLREKSIDPALKEAFGYALEHWADVDAAAKDNAAKIKTLLPTALDKACEGCKP
                     MLKQVSELQDYLLERSIWAIGGDGWAYDIGYGGLDHVMASNQNVNVLVLDTEVYSNTG
                     GQASKATPLGSIARFAEAGKETNKKDLGMMMMNYGYVYVAAIAMGANKAQALNAILEA
                     EAYPGPSIVIAYAPCINHGIDMSMSQKRQKLAVDAGYWLLYRYNPLNILQGKNPLTLD
                     SKEPSVSVQEFFKGETRYSALERIFPERSERFQGEAEVFFKERYLQYKKLTEG"
     gene            2674..>4110
                     /locus_tag="PLB85_04320"
     CDS             2674..>4110
                     /locus_tag="PLB85_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU72587.1"
                     /translation="MKAMKYFMLLVLAAATSLGALPLSIFYTGDSHGVFESKWDEASG
                     MNRGGYLVLEEILTAARSANPRSVYLDSGDQQTGSIFASLVDDNVHGGAVIEVFNRLK
                     LDASTFGNHEFDFSYSNTRDLTARANYPFVCTNLLDKSTRQPVGGHPYAIIEKDGLRI
                     GVFGITLELLPEKVKAQNVSSVRILPPADAINMYLDEVDRKSDLIVVLSHQGFEADSL
                     LAESLDDRVDIIIGGHDHIKAETPFFINGKYLLYSGSHLNWLGQAELEVEDDRVASLS
                     NQLIPLETSSKVFISPLAEYISQKIALVQVQMQRVIGYLPETWVPDKYQSTALSRWVA
                     NALRNEYMDVYKPDLAIINNGGLRKKIPAGAVTLRDLHELAPFNNTVTVFSCRGSDLI
                     LLDEINLRHAVEKPHDICEVAGLGFDTQVIKSQSSDDDLPWQNRYTVNGEPLVPERVY
                     RVVSHDYVAGQWDKYLGFKPFDVYDTGEL"
BASE COUNT         1006 a         1253 c         1069 g          782 t
ORIGIN      
        1 agatcatcgg tgacaacacc gatctctatg tgcagggcta tttccaatac gattcgaaga
       61 agagcggcgg catcacccgc agccacctcc gcttcggcaa aaaacccatc caaagccagt
      121 atttcgttac caaggaaaac tttgtagcct gccataacca ggctttcatc ggtcgcttcg
      181 acctccttgg cggcatcaag gaaaacggcg tcttcctgct caattcaaac tgggaacgcg
      241 aagaagcctt caataacctc acctccgaca tgcagcagat catcatcgac cgcaagatca
      301 agttctacaa catcgacggc ctcaaaatcg ccgaagcggt gggcttgggc ggacgcgtta
      361 acacagtgat gcagacggcc tttttcctta tatccggcat cctggagcgc ggcgaggcca
      421 tccagctgat caaggaatcg gtgagaaaaa cctacggccg caagggagag gaagtggttc
      481 agatgaacct aaaagccatt gaccacgtga acgaggccct caaggaagtg gaaatcccag
      541 cgacccttcc agaccagtgc gctccgcgga aaaaactggt cccggacggc tcggatgcct
      601 ttgttacagg cgttatcgag cccatcatgc gcgaagaggg agaccggatc aaggtttccc
      661 agatgcccat cgacggctat gtccccagcg gcacagccaa gctggaaaaa cgccgcgtgg
      721 ctccgaccgt gccccactgg atcgctgaaa actgcatcca gtgcaaccag tgctccttcg
      781 tttgccccca cgccgccatc cgtgccaaac ttgtcaaaga tgaagacctg ggcaacgccc
      841 cggccagttt caacactctt aaagccaccg gcgctgaagg ctaccaatac aaggtgcagg
      901 tctatatcga cgactgccag agctgccgcg tctgcgtaaa cgaatgcccc aaagccgctt
      961 tggaaatgcg ccccatcgag gacgagcgcg acgccggcga acaggccaac tacgaatact
     1021 ttgaagccct gccttccgat gtgctggcca ctttcaagga aaccaacgtc aagggttccc
     1081 agttcaagca gcctctgctg gaattctcag gagcctgcgc tggctgcggt gaaacgccct
     1141 acatcaagct gctcacccag ctttacggcg accgcatgat cattgccaac gccaccggct
     1201 gctcctccat ctggggcggc accttcccca ccatccctta cgccgtcaac aaggacggca
     1261 aaggtcccgc ctgggcgaac agcctctttg aggacaacgc cgaatacggt tttggcatgc
     1321 gtctggccgt gaattcccac cgcaagctgc tacgccatta catggaacag ctgcgcgaaa
     1381 aatccatcga ccccgcgctg aaagaggctt tcggctacgc cctagaacac tgggcggatg
     1441 tggatgccgc cgccaaagac aacgctgcca agatcaaaac cctgcttcct accgctctgg
     1501 acaaagcttg cgaaggctgc aaacccatgc tgaaacaggt ctccgaattg caggattacc
     1561 tcctggaacg ctccatttgg gcgatcggcg gtgacggctg ggcttatgac atcggctacg
     1621 gcggactcga ccacgtgatg gcctctaacc agaacgtgaa cgtcctggtg ctggacaccg
     1681 aggtctattc caacaccggc ggccaggctt caaaagccac gcccctgggc tccatcgccc
     1741 gtttcgccga ggccggcaag gaaaccaata agaaagacct tggcatgatg atgatgaact
     1801 atggctacgt ctatgtggct gccatcgcca tgggcgcaaa caaagcccag gccctgaatg
     1861 ccatccttga agctgaggcc tatcctggac cttcaatcgt catcgcctac gcaccctgca
     1921 tcaaccacgg catagatatg tcgatgagcc agaaacgcca gaaactggct gtggatgccg
     1981 gttactggct gctctaccgc tacaatccgc tcaacatcct ccagggcaaa aaccccctca
     2041 ccctagacag caaagagccc agcgtcagcg tgcaggaatt cttcaaaggc gaaacccgct
     2101 actcagctct ggagaggatc ttcccggaac gctcggaaag attccagggc gaagcggagg
     2161 tgttcttcaa ggaaagatac ctccaataca agaaactcac cgagggataa tcccctcaca
     2221 agataagaaa atagctggcg gaagcaattc cgccggcttt cttataggct ctctttcaaa
     2281 tcgagcgggt agcctgtagg ccaggccctt agcgctcttt accatccctc tcccacgcct
     2341 cccgtcaaat ccccgcatca aatgcgggca ttatacggga ggcgaatccg aagcacagga
     2401 aagggcctta acggccggac tgggtcgctt ctttcttctg ctggtccctg gccatgcatt
     2461 gagcaagaga agaggcggaa cagctgcctc ctgtacaaga accggctccc gacagaagct
     2521 tcggcacaaa gacaagctcc cttttccggg gaacgccgaa tttcagcttg acacaatcaa
     2581 gctgggggat atcaggtaaa tgtccgtata aaaagacaga agcgcccccg catttggggg
     2641 ttggcttgat ctgcataaat caaggaatcc gggatgaaag caatgaaata ctttatgcta
     2701 ctggttttgg ccgccgccac cagtttgggc gccctgcccc tgagcatttt ctacaccggg
     2761 gacagccatg gtgtttttga atccaagtgg gatgaggcca gcgggatgaa ccgcggcggc
     2821 tacctcgttc ttgaggaaat cctgacggcg gcgaggtccg caaatccgcg ctccgtctat
     2881 ctggacagcg gggaccagca gaccggctcc atcttcgctt cgctggttga tgacaacgtg
     2941 cacggcggcg ccgtcatcga ggtcttcaac cgcctgaagc ttgacgccag cactttcggc
     3001 aaccacgagt tcgatttctc ttattccaac acccgcgacc tcaccgcgcg ggccaactat
     3061 cccttcgtct gcaccaacct gctggacaaa agcacgcgcc aacccgttgg cgggcatccc
     3121 tatgccatca tcgaaaagga cggcctgcgg atcggggtgt tcggcatcac cctggagctg
     3181 ctgccggaaa aggttaaagc acaaaacgtt agcagcgtgc gcatcctgcc cccggccgat
     3241 gccatcaata tgtatctgga cgaggtggac cgtaagagcg acctcatcgt ggtgctgagc
     3301 caccagggtt ttgaggccga cagcctgctg gcggagagcc tcgacgacag ggtggacatc
     3361 atcattggcg ggcacgacca catcaaagct gaaacacctt ttttcataaa cggcaaatac
     3421 ctgctctaca gcggctcgca tctgaactgg ctgggacagg ctgagttaga ggtcgaggat
     3481 gaccgcgtgg cttccctttc caaccagctc atccccctgg aaacgagctc caaagtgttc
     3541 atcagccccc tggccgagta tatttcccaa aagattgctt tggtgcaggt gcagatgcag
     3601 agggtaatcg gctatctgcc agaaacatgg gtcccggaca aataccagtc cacggccctt
     3661 tcgcgctggg ttgcgaacgc cctaagaaac gagtatatgg atgtttacaa acccgacctg
     3721 gccattatca acaacggcgg cctgcgcaaa aaaatccccg ccggggctgt gaccctgcgc
     3781 gacctgcacg aactggcacc cttcaacaac accgtcaccg tcttttcctg ccggggaagc
     3841 gacctcatct tactcgacga aatcaacctc aggcacgcgg tggaaaaacc gcatgacatc
     3901 tgcgaagtgg ccggtttggg cttcgacacg caagtgataa aaagccagtc ctccgacgac
     3961 gacctgccct ggcaaaaccg ctataccgta aatggcgaac cgcttgtccc cgaaagagtt
     4021 taccgcgtgg tttcacacga ttatgtggca ggacaatggg acaagtatct gggcttcaag
     4081 ccgttcgacg tgtacgacac aggcgagctg
//
LOCUS       DAOOWR010000138         4093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21949_length_4093_cov_3.779099, whole genome shotgun sequence.
ACCESSION   DAOOWR010000138 DAOOWR010000000
VERSION     DAOOWR010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21949_length_4093_cov_3.779099"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..695
                     /locus_tag="PLB85_04325"
     CDS             30..695
                     /locus_tag="PLB85_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72588.1"
                     /translation="MICLAGHSLYKTYADKGQNIRVLIDASLEVEDGELVCITGKSGC
                     GKSTLLHILGLLDDPDSGKVFINGAEVSARHVNAHQIRNWDIGFVFQFHYLIEDLTAR
                     ENVALPMLIAGTGRQKALAEADNFLVTLRLSERGNHYPNQLSGGEQQRLALARALINR
                     PRIVLADEPTGNLDPQHSAEVWDLFLQLNRDLKQTFVIVTHDRELARQSPKTYELRDG
                     KLF"
     gene            700..>4093
                     /locus_tag="PLB85_04330"
     CDS             700..>4093
                     /locus_tag="PLB85_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72589.1"
                     /translation="MRRSRKFLSFIVVLLAINTLFFVSWYVFDVQGRVKGIAENEAGK
                     ALKGEFRIGFFTISSQQVYAEKISFAAADSLLNFTVDNARVRFNLLRFIFSGFKMRNL
                     MDHIEIDKADVQLAVVPKAKKAKKKFEIPDLKPIFNKLTITNSSFKADVKVPLKLGEE
                     GLLTVMEELSQVDLTIHNTNVSNLGLSATTSSRGTLNLNGILDKGRIASSRVELRNYE
                     PLYLSHPQIRDFRGNLDLAANVSQPKKGSDPVLNAHLVLKDASAFVWQQYPVNIPLLD
                     ITSDGSRLNASLAQSTVGSSSISGDIRARDLFGDPIIDSGQVDLRVDLSMLGANFSGW
                     VDGTLNAEGKLSDPVLQLAAASNKLSFSGQTVENVDLIATYKFKEVDLALRDLIWQNQ
                     RVDLTGSFGTEERKLTAKLDTEPARRGIADLKVTASADVELDFYSAVPEIRALIHGLD
                     FAQNKLDYRGLSGYANLVPLITDNGQNYLVDAELRSPLGIELSLVGDINDRNFLLEGE
                     LNSVQLASVYPDKSIEQFRPLLSGGISAFLAGSKAVLSGALSVDLTKDLNIDADIDLI
                     ASYDLKTKQGDLVLDTPYFTINGQPLTLGLVADIQDQELKISSFNLNKQIMASGGLDL
                     KDIQDMQFQVVLADIGSEMLQSFFPNLQLPQISGVNLTASYSSRGTDFLDAVLSVGEV
                     NIPGLKPMSAELTLLGDPRQVNIQGAIRNESTQLVDLVGDAVLDKGWNLRLNALATEL
                     NTAELMYSPPVEGLVSGNIGLFIADVLTGEREFSFDARLLSSRLSIPNVADLDDVLVS
                     VAQTKNLLIVDTLSVRSSEFGSLSGSGALDYNILNRTIFEGNHTLNLRAEGMLFEWLD
                     KEYEYVREASGKANLECSIKTLDDQLMVQSGVLRISEGRLVLDDQPEDIRNIELSADI
                     EENRVTIRNLTAQMGNGVLHAINHFDGDPESHLKAAMLDLGTLLVKVDEPGALLYVPD
                     ITAPRSRARIVLRGQNAEYATITGPFDDMMITAEALVSEASIVYPPRTNNLLNLIYSF
                     RGSLGMDSEPKREAIPLPFNLDLKVFVQDNVKYATYPTNFMIMPGGYMHILFDGSVWH
                     PQESSITSEQGSMDFFGTKFAMKELNFSITGAQKMVTIEGALT"
BASE COUNT          960 a         1158 c         1089 g          886 t
ORIGIN      
        1 cctgttgcgt aatacttagg aggtgagaat tgatctgttt agccggccat tcgctgtata
       61 aaacctatgc cgataaaggc cagaacatcc gcgtgctgat tgatgcctcc ctggaagtgg
      121 aggatggcga actggtctgc atcacgggga aatcgggctg cggcaaaagc actctgctcc
      181 acattttggg gcttctggat gatcccgaca gcggcaaggt gttcatcaac ggcgccgaag
      241 tctccgcccg tcacgtcaac gcccatcaga tccgcaactg ggatatcggc tttgtttttc
      301 aattccatta ccttatcgaa gacctcactg ccagggaaaa cgtggccctt ccgatgctta
      361 tcgccggaac tggtaggcaa aaagctttgg ctgaagctga caactttctg gttaccttga
      421 ggttatcgga acgcggcaac cactatccga accaactcag cggtggtgaa cagcagcgcc
      481 tggccctggc ccgggctctt ataaaccgtc cccgtatcgt cttggcggac gagcccacgg
      541 gcaacctgga tccccaacac agcgccgagg tctgggacct tttcttacag ctaaaccgcg
      601 atttgaagca gacctttgtg atcgtaaccc acgatcggga actggccagg caaagtccca
      661 aaacttatga actgagggac ggcaaactgt tctgacgcca tgcgacgctc ccgcaaattc
      721 ctgagcttca tagttgtcct actggccatc aacaccttgt tttttgtatc ctggtatgtc
      781 tttgacgttc agggcagggt gaaggggata gcggaaaacg aggccgggaa agctctgaag
      841 ggagagttcc ggatcggatt cttcaccatc agcagccagc aggtttacgc ggaaaagatc
      901 agtttcgcgg cggcggatag cttgctgaat tttacggtgg acaatgcacg ggtgcgtttt
      961 aatctgctgc gcttcatctt ctccgggttc aagatgcgca atttgatgga ccacatcgag
     1021 atagacaaag cggatgttca gctggccgtg gtgcccaaag caaaaaaagc caaaaagaaa
     1081 ttcgaaatcc cggacctcaa acccattttc aacaagctga ccatcactaa ttccagcttc
     1141 aaggcggatg taaaagtgcc gctcaaactc ggcgaagagg gtctgctgac cgtgatggag
     1201 gaactcagcc aggtagattt gaccatacat aacacaaacg tttccaacct tggcctttcc
     1261 gccaccacct ccagcagagg gaccctgaat ctgaacggga ttctggataa ggggcggatt
     1321 gcctcttccc gggttgaatt gaggaattat gaacccctct accttagcca tccccaaatc
     1381 cgcgatttcc gcggcaacct agacctggca gccaatgttt ctcagccgaa gaaaggctcg
     1441 gatccggtcc tgaacgccca tctggtgctg aaggacgctt ccgcctttgt ttggcagcaa
     1501 tatccggtga acatacccct gctggatatc acctcagacg gcagcaggct gaatgccagc
     1561 ctcgcccaaa gcacggttgg ctccagcagc atcagcggcg acatccgcgc ccgcgacctt
     1621 ttcggcgacc ccatcatcga ttccggccaa gtggacctgc gggtcgattt gtccatgctg
     1681 ggcgccaatt tcagtggctg ggtggatggc accctgaacg cggaggggaa actttcggac
     1741 cccgtgttgc aactggcggc ggcttcgaac aagctttcct tctccgggca aaccgtggaa
     1801 aacgttgacc ttattgctac ttacaaattc aaagaggtcg acctggcctt gcgggaccta
     1861 atttggcaaa accagcgggt ggacctcacc ggctcgttcg gcacagaaga acgcaaactg
     1921 accgccaagc tggatacaga accggcacgg cggggcattg ctgacctcaa agtgactgcc
     1981 tcagccgatg tggaactgga tttttacagc gcggtgccgg agattcgggc cctgatccac
     2041 ggtctggatt tcgcccagaa caaacttgat tatcgcgggc tctccggcta cgcgaatctg
     2101 gttccgctca tcacagataa cggccagaac tacctggtgg acgcggaact cagatcgccg
     2161 ctgggcatcg agctcagcct ggtgggagat atcaatgacc gcaatttcct gctggaaggc
     2221 gagttaaact cggtgcagct tgcctccgtc tatcctgaca aaagcatcga acaattcagg
     2281 ccgttgctga gcggcggaat ttccgccttc ctcgccgggt ccaaagccgt gctgtcaggt
     2341 gctttgtcag tcgatttgac caaggacttg aatattgacg cagacatcga cttgatcgcg
     2401 tcttacgacc tcaaaacaaa acaaggcgat ttggttctgg acacgccata ctttactatc
     2461 aacggacagc ccctcaccct gggtctggtg gccgatatcc aagaccagga gcttaaaatc
     2521 agctctttta acctgaacaa gcagatcatg gccagtggag gactggacct caaagatatc
     2581 caggacatgc aattccaagt ggttttggcc gacattggca gcgagatgct ccagtctttt
     2641 ttcccgaacc tgcaattgcc ccagattagc ggtgtgaacc tcaccgccag ctattccagc
     2701 cgtggcaccg atttcctgga tgctgtgctg agcgtcgggg aggtcaatat tcccggcttg
     2761 aagcccatgt cggctgaact gacgctgctg ggggacccaa gacaggtaaa cattcagggc
     2821 gcgatcagaa atgaatccac ccaactggtc gatctggtcg gagatgccgt gctggacaag
     2881 ggctggaatc tgcgcctgaa cgccctggcc accgaactga acacagcaga gctgatgtat
     2941 tcgccgccgg tggaaggttt ggtatccgga aacatcgggc ttttcattgc tgacgtgctg
     3001 accggggagc gtgagttctc tttcgacgcc agactactgt cttcgcggct ttcgatcccc
     3061 aacgtcgcgg atctggatga tgtgcttgtc tctgtggccc aaaccaaaaa cctgctgatc
     3121 gtggacaccc tctccgtgcg cagcagcgag ttcggcagct taagcggttc cggcgctttg
     3181 gattacaata tcctgaaccg caccattttc gaaggtaacc acaccctcaa ccttcgcgct
     3241 gaaggaatgc tgttcgaatg gctggacaaa gaatatgagt atgttcgcga agccagcggc
     3301 aaagccaacc tggaatgcag catcaagacc cttgatgacc agttgatggt gcagagcggg
     3361 gttctgcgga tcagcgaagg caggctggtt ctggatgacc agcctgagga tatccgcaac
     3421 atcgagcttt cagcggacat cgaagaaaac cgcgtgacga tcaggaacct caccgcccag
     3481 atgggtaacg gggtgctgca cgccatcaac cacttcgacg gcgatcccga atctcatctc
     3541 aaagccgcca tgctcgatct gggcaccctg ctggtaaagg tggacgagcc cggcgcgctg
     3601 ctttacgtgc cggacatcac cgctccccgt agccgcgcac gaatcgtttt gcgtgggcaa
     3661 aatgctgaat acgcgacgat tacagggcct ttcgacgaca tgatgataac ggctgaggct
     3721 ttggtgagcg aagccagcat cgtttatccg ccccgcacca acaacctgtt gaacctgatt
     3781 tacagcttcc gcggttcttt gggcatggat tccgagccca agcgcgaagc catacccctg
     3841 cctttcaacc tggacctcaa agtgtttgtg caggataacg ttaaatacgc cacctatccc
     3901 accaacttca tgatcatgcc cggtggttac atgcacatcc ttttcgatgg cagcgtctgg
     3961 catccccagg aatccagcat cacttccgag cagggcagca tggatttctt cggcaccaag
     4021 ttcgccatga aagagctgaa cttcagcatc accggagccc agaaaatggt gaccatcgaa
     4081 ggcgccctca ccc
//
LOCUS       DAOOWR010000139         4089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_21976_length_4089_cov_4.088250, whole genome shotgun sequence.
ACCESSION   DAOOWR010000139 DAOOWR010000000
VERSION     DAOOWR010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21976_length_4089_cov_4.088250"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="hutI"
                     /locus_tag="PLB85_04335"
     CDS             <1..918
                     /gene="hutI"
                     /locus_tag="PLB85_04335"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOU72590.1"
                     /translation="GTIRTTRDASEDLLFELAAKRIRKMIALGATTVEAKSGYGLDTA
                     SELKQLRVIARLNKELPVTVVPTFMGAHEFPEEFKDNHAAYVKILCEDMIPAVAEQGI
                     ARFCDIFTEAHVFGIEESRIVLNCARDHGLSLKMHADEIEPIGGAELAAELGCVSADH
                     LGAASDAGIQAMKEAGVIPVLLPATLYSLASKKYARGRHMIDCGLPVAIATDYNPGSC
                     NCDSLPFTMSLACLQMGLNPVEALCACTINAAHALGLGAETGSLEPGKLADFCIWDIP
                     SLNFIPYHLGSSYITAVYARGKLVHTSSE"
     gene            915..2870
                     /locus_tag="PLB85_04340"
     CDS             915..2870
                     /locus_tag="PLB85_04340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOU72591.1"
                     /translation="MNGNSGENAYTIIETLHKSRQFCVYKARNYFSGRIVTIKTNEQR
                     FRSDADQVGQLRDEAETGLRLRHPSIRETIGLFEDGGTVYMVSEYLEGDPLNDILRIP
                     RVDISYSQAIKWTQQLLDALEHAHKHLILHLNLNPTNIIITSDYDLKVLGFGKSPHAW
                     KNADPEQHAFHPVLFTSPEVFSEKNPDERSDIWSAAVISWLLLRGRLPWELDRRDSPS
                     IQKEQTLAQYPLSPDQPGRHIPPWLFSILNKALQPDPARRFASAAEMREAIANQEEVA
                     LEIDGVGPASSKPGAIFPGDNQFSPLGVPAINSEPSLSDTQPGIGRQSRGVDPEPVKR
                     PGDVTVEGPAHKIPIKSEAASAEIKKLQKTFRVLGIISACIFVYIILKYYVIRDGAIF
                     SRARHGDSPAQVADAKQKVANEPLEMISVLGETAVIGNNGPDAKSDEYPLLQVKLSSF
                     MISPREITREQWAMANPGYKVCGNDKDLPVTGITFDEVVAFCNEKSRLDNLEPCYEYL
                     GNGVSCDFNASGYRLPTEAEWEYVAKARRTDAFTPYSGSSVANVVAWYSENSGGSLHP
                     VGQKQPNQIGIYDLSGNAAEWVWNWYSSYASVTDLAFAGPDQGTDKVIRGGSFKDPAE
                     NIRVTSRAHGKPYAKADHIGFRVVRKK"
     gene            complement(3274..>4089)
                     /locus_tag="PLB85_04345"
     CDS             complement(3274..>4089)
                     /locus_tag="PLB85_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72592.1"
                     /translation="NLEPYEILDRFDEYVVVGEISHTLRTILRLKQRQGKRLVSVASC
                     GSKALMFADNVHSSIDSLSPSPRQLFIYNLNRISEQSVARVLNAAHKVDPALGNVLET
                     SDYQNYRGLLLTQPDFSLPEQTDFLLAWGAQTSMATCPGFRVEIQQFADPDSEADLLL
                     PGPSYLEIEGTALSNNGGITRFKNPARSNLSGELLRLFYELGWISPATADINHWNDAA
                     EQLLKAASANEPKAYDPAGIEINALRDMVLPLDNLLQQRIIMLYEKRKIPTGF"
BASE COUNT          948 a         1211 c         1092 g          838 t
ORIGIN      
        1 ggcacgataa gaaccacccg cgacgcttct gaggacctgc tcttcgagct tgccgccaaa
       61 agaatccgca aaatgatagc gttgggcgcc accaccgtgg aagccaaaag cggatacggc
      121 ctcgatacag ccagcgaact gaaacaactg cgcgttatcg cccggctgaa caaggaattg
      181 cccgtaaccg ttgttcccac cttcatgggc gcgcacgaat ttcccgagga attcaaagat
      241 aaccacgcgg cctatgtaaa aatcctctgc gaagatatga tccccgccgt ggctgagcaa
      301 ggcatagccc ggttttgcga catcttcacc gaagcccacg ttttcggcat tgaagagagc
      361 aggatcgtgc tcaattgcgc cagagaccac ggtttgtcgc tcaaaatgca cgctgatgag
      421 atcgagccca tcggcggagc ggaactcgcg gctgagctgg gctgcgtgtc agccgaccac
      481 ctgggagccg cttccgacgc gggtatccag gccatgaaag aagccggcgt gatccccgtt
      541 ctgctgcccg ccacgctcta ttcgctggcc agcaagaaat atgcccgcgg ccgccacatg
      601 atcgattgcg gcctgccggt ggcaatcgcc acggattaca atcccggcag ctgcaactgc
      661 gattcgctgc cttttaccat gagcctggcc tgcctgcaga tgggcctgaa tcccgtggaa
      721 gcgctctgcg cctgcaccat caatgccgcc cacgcccttg gcttgggggc agagacaggc
      781 tcgctggagc cgggaaaact ggcggacttc tgcatctggg acatcccttc cctgaacttc
      841 attccctacc atctgggatc atcgtatatc acagcagttt acgccagggg aaagctggtc
      901 cacacctctt cagaatgaac ggcaatagcg gcgaaaacgc ttacaccatc attgaaaccc
      961 tgcacaaatc gaggcagttt tgcgtctata aagcccgcaa ctatttcagc ggcagaatag
     1021 taaccatcaa aaccaacgaa cagcgcttcc ggagcgacgc tgaccaggtg ggccaattgc
     1081 gcgacgaagc ggaaaccggc ctccggctga gacatccaag catacgtgaa accataggtc
     1141 tgtttgagga tggcggcacc gtttacatgg tcagcgaata ccttgagggc gatcctctca
     1201 acgatattct gcgcatcccg cgcgtggaca tcagctacag ccaggccatc aagtggaccc
     1261 agcagttgct ggacgctctt gaacacgcgc acaaacacct tatcctgcat ctgaacctta
     1321 accccaccaa catcatcatc acatcggact atgacctcaa ggtgctgggc ttcggcaaat
     1381 cgccccacgc gtggaaaaat gccgatcccg agcagcacgc tttccatccc gtgcttttca
     1441 cctccccgga agttttttcg gagaaaaatc cggacgaacg ctcggacatc tggtccgccg
     1501 ccgtgatttc atggctgctg ctgcggggca gactgccttg ggaacttgac cgtcgtgaca
     1561 gcccctccat ccagaaagag caaacattag cccaataccc gctcagtcca gaccagcccg
     1621 gaaggcacat ccccccctgg ctcttcagta ttctcaacaa ggctttgcag cccgatcccg
     1681 ccaggcgttt tgccagtgcc gcggaaatga gggaggcgat cgccaaccag gaggaagtgg
     1741 ctctcgaaat tgacggagtc gggcccgcat catcaaaacc aggggcaatc tttcctggcg
     1801 acaaccaatt ctccccgctc ggcgttcccg ctattaattc tgagccttcc ctgtctgata
     1861 cccagccagg aatcggcaga cagtcccggg gtgttgaccc cgagccggtc aagcgtcccg
     1921 gagatgttac agttgaaggt ccagcccata aaatccccat taaaagcgag gctgcctctg
     1981 cggaaatcaa gaaactgcag aaaaccttcc gcgtgctggg aatcatttca gcctgcatct
     2041 ttgtttacat tatcttgaaa tactacgtga tcagggatgg ggcgattttc agtcgcgccc
     2101 ggcatggcga ctctccagca caggtggccg acgcgaagca gaaggtggca aacgagccac
     2161 tggaaatgat ctcagtcttg ggagaaacag cggtcatcgg caacaacggc cccgacgcca
     2221 aaagcgacga gtatccgctg ctgcaggtga agctgtcttc gttcatgatc tcaccccgtg
     2281 aaatcacgcg tgaacagtgg gccatggcca atcccggcta caaggtttgc ggtaatgaca
     2341 aggacctgcc agtcactggc atcacctttg atgaggtggt ggctttctgc aacgaaaaga
     2401 gccgccttga caatttggag ccctgctatg aatatcttgg caacggcgtg agttgcgatt
     2461 tcaacgccag cggataccgc ctgcctactg aagcggagtg ggaatacgtc gccaaagcca
     2521 gacgcacaga tgctttcacc ccttacagcg gttcctctgt cgcgaatgtg gtggcctggt
     2581 attccgaaaa cagcggcggc agcctccatc ccgttggcca aaaacaaccc aaccaaatcg
     2641 gaatctacga cctcagcgga aacgcggcgg aatgggtctg gaactggtat tcgtcctacg
     2701 cgagcgtaac ggacctggct tttgccggac ctgaccaggg caccgacaaa gttatccgcg
     2761 gcggttcgtt caaagacccc gctgaaaata tccgcgtcac ctcacgcgcc cacggcaaac
     2821 cctatgccaa agcagaccat atcggctttc gcgtggtgcg gaaaaagtag gtctttcatt
     2881 caaaccggat aagcaaagtc cttcggtcct ctttctgttt gagcgtccgc attttcttaa
     2941 tttgatggag agccataagc ccaccccaat atcctgccag ccagaccctt agcgcctgtg
     3001 tccatccctc tcccccgccc ctcgccaaat cctcgcatca aatgcgggca tcatgcggga
     3061 ggcgcatccc aggcacagga tagggcctta atggccagac tacatcgctt ctcctcagca
     3121 acttcacacc ctcgctatcg tctatcgttc caaagcgtta tcgacccgat ttgaaagaga
     3181 gccaaaagca gtctgcgcca aacccgggtt acaaaaaagc ccggcatcag gccgggcttg
     3241 tgatataggg ccgggttcat ccgcaaggat gcttcagaat ccggtgggaa ttttgcgctt
     3301 ttcgtaaagc atgataatgc gttgctggag caggttgtcc agaggcaaca ccatatctcg
     3361 cagagcgtta atctcgatcc cggcaggatc gtaagccttg ggctcatttg cggaagctgc
     3421 tttcagcagt tgctccgccg cgtcgttcca gtggttgatg tccgccgtgg cgggtgaaat
     3481 ccatcccagt tcgtaaaaga ggcgcagcaa ttcgccgctg aggttggaac gggcggggtt
     3541 tttgaacctg gtgatgcctc cgttgttgct cagcgcggtg ccctcgattt ccagatatga
     3601 ggggcccgga agcaacaggt cagcttcgga atcggggtcc gcgaactgct ggatttcaac
     3661 cctgaagcct ggacaggtgg ccatagatgt ttgagcgccc caggcaagca aaaagtccgt
     3721 ttgttccggc aggctaaaat ccggttgcgt cagcagcaag ccgcggtagt tctggtaatc
     3781 cgaggtttcc aacacgttgc ccagagccgg atcgaccttg tgggcggcgt ttagcaccct
     3841 ggcaacgctt tgctcgctga tcctgttcag gttatagata aagagctggc gtggcgatgg
     3901 gctgagcgag tcgatagagg aatgaacgtt atccgcgaac atcagagctt tggagccgca
     3961 actggctacg ctgacaagcc tcttgccctg acgctgtttt aggcgcagaa tggtgcgcag
     4021 ggtgtggctg atctctccca ccaccacata ttcatcaaag cggtcgagaa tttcgtaggg
     4081 ctcgagatt
//
LOCUS       DAOOWR010000140         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22098_length_4073_cov_3.459433, whole genome shotgun sequence.
ACCESSION   DAOOWR010000140 DAOOWR010000000
VERSION     DAOOWR010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22098_length_4073_cov_3.459433"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1835
                     /locus_tag="PLB85_04350"
     CDS             <1..1835
                     /locus_tag="PLB85_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72593.1"
                     /translation="RLKVNYQVNKTDNQNISNIYFEFFEPGKDTAFAKVNEFHQDNII
                     YVNIPKKYSLKNGGKVPEDSLQVKITMAVKPYKINPKNGRITYGKKELLKDYTMVQYI
                     KPDPAMMAYMPTRGLYLNGVDVSQVQNVVYRKGYRSSTYSLVKSSTSYENNLDVTIGY
                     ISTVTKPIAGFNFVKGDSLYTYMDGKRFSRKLAAGEKARDLALVSSEIDFTYDQESKT
                     ILNTSIYLDEAKKIISDLKTDLDDAKKELAAYGDSLAQAKVPEAAMVYQGAVARLTQK
                     VEGLEEKVKAYSGNKLFQEALKQKSDRGLMRLVSSIREPESRQHSYMIVRTNGEGLFS
                     ETAPDTLKTGEYVNYAPISNWFDWNKLITLFAVLIFGIMVVVFVNLAKKGKDLYMRPI
                     AGLQEIDNAIGRATEMGRPMLYCMGNGGLSDVATLASMGILSLVAKKAAEYDTKLIVP
                     CYDYIVMPIAQEIVREAHYAVGRPDSYDKNNVFYLTSVQFAYVAGVNGIMIREKMATN
                     FFMGYFAAEALLMTETGNAVGAVQIAGSDAITQIPFFITTCDYTLIGEELYAASAYLN
                     REPMLLGTLKAQDYFKFLILVFVIVGALTATFQLTGIMQAFPLK"
     gene            1848..2474
                     /locus_tag="PLB85_04355"
     CDS             1848..2474
                     /locus_tag="PLB85_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72594.1"
                     /translation="MKKTIPLLIVIIGGFIWLAWVFVPHRLLQDVFYYEFYVPWARAM
                     APFGMLLGVMSLSMMHYGKITRKAPKWQYSFFFFAGFIVTSLAGFIGGTQKGGLYMWL
                     FENMQMPMSATMFSLLAFYMASAAYKAFRARSPEATVLLVAAIVVMLAQVPLGVKISK
                     FLPDISQWILDVPNLASKRGIMLGVGLGSVATTLKLLLGIERSYLGGD"
     gene            2477..3670
                     /locus_tag="PLB85_04360"
     CDS             2477..3670
                     /locus_tag="PLB85_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72595.1"
                     /translation="MTEKLNFFERLQKLDRRWIYIVVALAIIIPLMLPYNSDNVTSPP
                     TENVYQMVDSFAGREDRAILMSFYHDASTMPELYPMQIAILRHCFERNVKVFFLSFLP
                     TGAPIIDMAINTVKEEYPNIKSGTDYCNFGYIPSALIMPTIIGMGDNIATAVPTDAEG
                     RKVENLPIMKGINNYTEMNLVIEFSGSVAGGYWLTYARPKFGTNIAVGVTAVMAADEY
                     PYLQTGQFIGMLAGLKGAAEYEKLVEVFAAYRAPGDVMHPYTDEEGNKILPGRSFSDD
                     ILEKPEVQDLVKITTQTQAKFTPEEFQKFVVNYPEEDRVKLTQAKQEADGKIVIDVSD
                     LEENDFAPTTWDALNRMTRNTIYKFKVARIGMNAQSVAHIMIIVFILIGNIGYFIQKA
                     RLRKQ"
     gene            3683..>4073
                     /locus_tag="PLB85_04365"
     CDS             3683..>4073
                     /locus_tag="PLB85_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72596.1"
                     /translation="MTFELFSNLVGAFFTLCIFSFLYKDNPFYQMSEQLLVGISLGYS
                     LVLTYERVFIPYFYQPIFLKHEWILIIPSIIGMFYLLRFSRKLSWLSRYPIAFSMMGV
                     GASVALAMHNSILVQMRQAMVPIENVNL"
BASE COUNT         1019 a         1175 c          997 g          882 t
ORIGIN      
        1 ccaggctgaa agtgaactac caggtgaaca agaccgacaa tcagaatatc agcaacatct
       61 atttcgagtt cttcgagccg ggcaaagaca ctgcctttgc taaggtaaac gaatttcacc
      121 aggacaacat catctatgtg aacattccca aaaaatactc cctgaaaaac ggcgggaaag
      181 ttcccgaaga cagtctgcaa gtgaagatca ccatggccgt gaagccctac aagataaatc
      241 ccaaaaacgg ccgcatcacc tacggcaaaa aagagctgct caaagattac acgatggtgc
      301 aatacatcaa accggacccc gccatgatgg cctacatgcc aacgcggggg ctgtatctga
      361 acggcgttga cgtctcccaa gtgcaaaatg tggtttaccg caaaggatac aggagttcta
      421 cctacagtct ggtgaagagc agcacctcct atgaaaacaa cctcgacgtg accatcggct
      481 acatatccac agtaaccaaa cccatcgccg gattcaactt tgttaaaggc gattcattat
      541 atacatatat ggatgggaaa cgcttctccc gcaaactggc ggcaggagaa aaagcccgcg
      601 acctcgctct ggtctcctcc gaaatcgatt tcacctacga ccaggaaagc aaaaccatac
      661 tgaataccag catctatctg gacgaggcga agaagataat cagcgatctc aagaccgatc
      721 tggacgacgc caaaaaagaa cttgccgcct atggagattc cctggcccaa gccaaagtgc
      781 cagaagccgc tatggtctat cagggcgctg tcgcccgcct cactcaaaag gtggaaggcc
      841 tagaggaaaa ggtaaaggca tacagcggca acaagctttt ccaggaagcc ctgaagcaga
      901 aaagcgaccg cgggctgatg cgcctggtgt cctccatccg cgagccggaa tcacgccagc
      961 acagctacat gatcgtgaga accaacggcg aaggcctttt ctccgaaacg gcaccggaca
     1021 ctctcaagac cggggagtat gtaaactacg cgccgatctc caactggttc gactggaata
     1081 agctcatcac cctatttgcg gtgctcatct tcgggatcat ggtagtggtg tttgtgaacc
     1141 tggccaagaa gggcaaagac ctctatatgc gtcccattgc cggcttgcag gaaatcgaca
     1201 acgccatcgg ccgcgccacc gaaatgggac gcccgatgct ttactgcatg ggcaacggcg
     1261 ggctttcaga cgtggcgacc cttgcttcca tgggcatcct gagtttggtt gccaaaaagg
     1321 ccgctgaata tgacactaaa ctgatagttc cctgctacga ctacatcgtg atgcccatcg
     1381 cccaggaaat cgtacgggaa gctcactacg cggtggggcg cccggacagc tatgacaaaa
     1441 acaacgtttt ctacctcacc tccgtgcagt tcgcctatgt ggcgggagtg aacggcatta
     1501 tgatccgtga gaagatggcc accaacttct tcatgggtta tttcgccgcc gaagctctgc
     1561 tgatgacgga aacgggtaac gccgtcggag ccgtgcagat cgccggctcg gatgccatca
     1621 cccagatccc gttctttatc accacttgcg actacaccct catcggagaa gagctttacg
     1681 ctgcctccgc ctatctgaac cgtgaaccga tgctgctggg aaccctcaaa gcccaggact
     1741 acttcaagtt cctgatcctg gtttttgtga tagttggcgc tttaacggct acattccagc
     1801 tgactggcat aatgcaagct ttccccttaa aataaaagag gtcttagatg aagaagacaa
     1861 ttcccctgtt gatagtgata atcggaggat tcatctggct agcctgggtg ttcgtgcccc
     1921 acaggctgct gcaggatgtt ttctactacg agttttacgt tccctgggca cgggcaatgg
     1981 cgcctttcgg catgctgcta ggcgtaatga gcctttccat gatgcactat ggtaagatca
     2041 cacgcaaagc tcccaagtgg cagtacagct tctttttctt cgccggattc atcgtcactt
     2101 cgctggccgg attcatcggc ggaacccaga aaggcgggct ctacatgtgg ctgttcgaaa
     2161 acatgcagat gccgatgagc gccacaatgt tctcgctgct ggccttttac atggcctccg
     2221 cggcttacaa ggccttccgc gccagatcgc ccgaagccac cgtgctgctg gtggcggcca
     2281 tagtggtgat gctggcccag gtgccgctgg gagttaagat cagcaaattc ttgccggata
     2341 tttcccaatg gatcctggac gtgccaaacc tggcctccaa acgcgggatc atgcttggcg
     2401 ttggcctcgg cagcgtggcc acaaccctga agctcctgct gggaattgaa cgttcctatc
     2461 tgggaggtga ttgatcatga cagaaaaact gaacttcttc gaacgcctgc aaaagcttga
     2521 ccgccgctgg atctacatag ttgtcgccct ggctatcatc attcctttga tgttacccta
     2581 taattcggac aacgtgacct ctccgcccac ggaaaacgtg tatcagatgg tcgattcctt
     2641 cgcaggacgc gaagaccgcg ccatcctgat gagcttttac catgacgcct ccaccatgcc
     2701 ggagctctat cctatgcaga tagccattct gcgccactgc ttcgagcgca acgtgaaggt
     2761 gtttttcctt tccttcctgc ccaccggcgc gcccatcatt gacatggcca tcaacaccgt
     2821 aaaggaagag tatccgaaca tcaagagcgg caccgactac tgcaacttcg gttacatccc
     2881 ctccgccctc atcatgccaa ccatcatcgg catgggcgac aacatcgcca ccgcggttcc
     2941 caccgatgct gagggccgca aggtggaaaa cctgcctatc atgaaaggca ttaacaatta
     3001 cacggaaatg aacctggtga tcgagttttc cggctccgtc gcaggtggct actggctcac
     3061 ctacgcccgt cccaaattcg gcaccaacat cgcggtgggc gttaccgccg tgatggccgc
     3121 tgatgaatat ccctacctgc agaccggcca gttcatcggc atgctggcag gcttgaaggg
     3181 cgccgccgaa tatgaaaaac tggtggaagt ctttgccgcc taccgcgctc ccggcgacgt
     3241 gatgcacccc tacaccgacg aagaaggcaa taaaatactg cccggccgtt ctttcagcga
     3301 cgacattcta gaaaaacccg aggtgcagga tttggttaaa atcaccaccc agacccaggc
     3361 caagttcacc ccagaagagt tccagaaatt cgtggtgaac tatcctgagg aggaccgcgt
     3421 taagctgact caggcgaaac aggaagccga cggcaagatc gtgatcgacg taagcgacct
     3481 tgaagaaaac gattttgccc ccaccacctg ggatgccctg aaccgcatga cccgcaacac
     3541 catctacaag ttcaaggtcg cccgcatcgg catgaacgcc caatccgtag cccacatcat
     3601 gatcattgtg ttcatcctga tcggaaacat cggctacttc atccagaaag cccgtctccg
     3661 caaacaataa agggaggaaa aaatgacatt tgaactcttt agcaaccttg tgggtgcctt
     3721 tttcaccctc tgtatcttct ccttccttta caaggacaac cctttctatc agatgtccga
     3781 acagcttctg gtgggaatat cgctgggcta ttcgctggtg ctcacctatg aaagggtgtt
     3841 catcccctat ttctaccagc ccatcttcct caaacacgag tggatactga tcataccaag
     3901 catcatcggt atgttctacc tgctgcgttt ttcccgcaaa ctcagctggc tgtcgcgcta
     3961 tcccatcgct ttttcgatga tgggtgtggg cgcttccgtc gcgctggcca tgcacaacag
     4021 cattctggtg cagatgcgcc aggccatggt gccgatcgag aacgtgaacc tgg
//
LOCUS       DAOOWR010000141         4051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22263_length_4051_cov_3.310310, whole genome shotgun sequence.
ACCESSION   DAOOWR010000141 DAOOWR010000000
VERSION     DAOOWR010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22263_length_4051_cov_3.310310"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLB85_04370"
     CDS             complement(<1..999)
                     /locus_tag="PLB85_04370"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU72597.1"
                     /translation="MEQLIKQTLNKLAQPDISFADVRITRTDNEWLYFMNGFMRSYGS
                     SMDSTAIGIRVLINGCWGFAGSRDLSAAAIDRLVRTAVRNARQGGSFIRNRVEFPALP
                     ATRAEYHHQPEIDPFKMPKEEKTACLRGLAEAIKPQGKIVHSMVMGQFMRQEKYYANS
                     EGTYSHTSYYNTLPVMEVVASDGGQIQSRTWPGHMSAGRGGFELFKQQKFAENTDRIM
                     KEAIDLLNAPTIEDDRADIIIGNGHLALQLHESVGHATEADRIFGMEISYAGKTFIKP
                     EMLGSFQYGSPLVNIYSDSTDPKGLGYHPVDDEGVPGQKVDIIKDGRIIDTMKINEL"
     gene            complement(1011..2336)
                     /locus_tag="PLB85_04375"
     CDS             complement(1011..2336)
                     /locus_tag="PLB85_04375"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOU72598.1"
                     /translation="MDQLEYLKSVLDQHPELQYSITQSNWSTDFLRFYHSETNYNISK
                     DNTTIGAEIYKGKKSFNFAIENPERGKIDAALRDALEVIDKLPEDPDFVDLESDMTLA
                     EPRVVKNNIEAIPLDLKTKILSNISAAASMYDFDIFGTFICNYQSFRLINSNGLDKSS
                     QSSPIYLEVKAVHNKSQVTVLETFGGEDFGYFNERAFTESLMQKIKYCSSEIVDVEPG
                     YYDVILAPRCLAEFIQYLSHGMSARVLDQHSSWFEGKLNKQVFPAHISIVDDPADPRM
                     IRADYGSDGHIYRRLPLVEKGVFRAFLCNNYYAHKTGLPKNGNTASCLKIEPGDKTLE
                     EMIGGIKRGLYISSLHYMNFINPRETSLTGLTRDGTFLIENGRITKVVNNLRFTERIE
                     RILNNVVGLEDHCATIPFSGNYESFDIETVKAPHAHVRDFNVTSSTKTI"
     gene            complement(2357..3472)
                     /locus_tag="PLB85_04380"
     CDS             complement(2357..3472)
                     /locus_tag="PLB85_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU72599.1"
                     /translation="MPGAVDPHSHIITEQDPKKSIARISKAALSGGWTTLAELSFLNP
                     RPIFSLSDLKRLRDQIDQSSFVDMAVWGNVDITDYPYHAEAAQELWANGVVGIALANP
                     SPNPEIQALSFTEIMDLFMDIYESDTAFAFQGYDTDTAKKFSFDAQSDAIKKLLRRMQ
                     ENPIHITRVNAFPTVEFINSVHKRSDISFAMCVADMMLLFSQIDSGQRMDLADYQQEL
                     FDLLRTNKIYLLSNNVESASVPKDLDEAFHGTSEQLLPWSYLWVLSELWKKRRVPLAT
                     VIKMTSENAAKRLGIYPAKGCIEAGSDADFVIYDPNKDTDFVAPDGSSHTLKGSIDSV
                     WLRGKKVVNKGKVASAAGRALSRSTNPKRRHNSTTWI"
BASE COUNT          774 a         1001 c         1230 g         1046 t
ORIGIN      
        1 cagttcattg attttcattg tatcaatgat ccggccgtct ttgatgatgt ccactttctg
       61 gccgggaacg ccttcatcgt ccacaggatg gtaacccagg cctttggggt cggtgctgtc
      121 gctgtagatg ttcaccagcg gcgagccgta ttggaaggag cccagcattt cgggcttgat
      181 gaaggttttg ccggcgtagg agatttccat gccgaagatg cggtccgcct ctgtggcatg
      241 tcctaccgat tcatgcagtt gcagcgcgag atgcccgttt ccgatgatga tgtcggcacg
      301 gtcgtcctct atggtaggag cgtttagcag atcgatggcc tctttcataa tgcggtcggt
      361 gttttcggcg aatttttgtt gcttgaagag ctcgaagccg cctcggcccg cgctcatgtg
      421 gccaggccag gtgcgggact ggatctggcc tccgtcggag gcgaccacct ccatcaccgg
      481 tagggtgttg taatatgatg tgtgggaata agttccctcg ctgttcgcgt aatacttttc
      541 ctggcgcatg aattgcccca tcaccatgga gtggacgatt ttgccctggg gtttgatcgc
      601 ctcggccagg ccgcgcaggc aggcagtctt ttcttccttg ggcattttga aggggtcgat
      661 ctcgggctgg tggtgatatt ctgccctggt tgcaggcaaa gcggggaact ccaccctgtt
      721 gcggataaaa ctgccgccct ggcgtgcatt tcttaccgct gtcctgacaa ggcggtcgat
      781 cgcggcggcg ctcaggtcgc gcgacccggc gaaaccccag cagccgttga tgagcaccct
      841 gatgccgatg gcggtggaat ccatgctgct gccgtaactg cgcatgaatc cgttcataaa
      901 atacagccat tcattgtcgg tgcgggtgat cctgacatcg gcgaagctga tgtcaggctg
      961 ggcgagttta ttgagggttt gttttatcag ttgttccatg ttgcgctccc ctaaatcgtt
     1021 ttggttgatg aggttacgtt gaaatcccgc acgtgggcgt gaggcgcttt cacggtttcg
     1081 atgtcgaagc tctcatagtt tcccgaaaac gggatcgtgg cacagtggtc ttcaaggccc
     1141 accacattgt tgaggatgcg ttcgatgcgc tcggtgaaac ggaggttgtt cacgaccttg
     1201 gtgatgcgtc cgttctcaat caggaaagtg ccgtcgcggg taagcccggt gagtgaggtt
     1261 tcgcgggggt tgatgaagtt catgtagtgg aggctggaga tgtaaaggcc gcgtttgatg
     1321 ccgccgatca tctcctcgag ggttttgtcg ccgggctcga tcttgaggca ggaagcggtg
     1381 ttgccgtttt tgggcaggcc ggtcttgtgg gcgtagtagt tgttgcagag gaaagcccgg
     1441 aaaacgccct tctccaccag cggcaggcgg cggtagatgt ggccgtcgga accgtaatca
     1501 gcgcggatca tgcgcgggtc ggcgggatcg tcaacaatgc tgatgtgtgc gggaaaaacc
     1561 tgtttgttca gcttgccctc aaaccaactg ctgtgctggt ccaggactct ggcgctcatg
     1621 ccatgggaca ggtattggat gaattccgcc aggcagcgcg gggccaggat cacgtcataa
     1681 tatcctggct caacgtccac gatttcggag gagcagtatt tgattttctg catcaggctc
     1741 tcagtgaagg cgcgttcgtt aaaatagccg aaatcctcgc cgccgaacgt ttccagcaca
     1801 gtcacctggg atttgttgtg cacggctttc acctccagat agataggcga gctctggctg
     1861 cttttgtcca ggccgttgct gttgatgagg cggaaggact gataattgca gatgaaggtg
     1921 ccgaagatat cgaaatcgta catggaggcc gcggcggaga tattggacag tatttttgtt
     1981 ttcaggtcca gcggaatggc ctcgatgttg tttttcacca cgcggggttc ggccagagtc
     2041 atgtcgcttt ccaaatccac gaaatccggg tcttcgggaa gtttgtctat cacctccaga
     2101 gcgtcacgca gggcggcgtc gatcttgccg cgctcggggt tttcgatagc gaagttgaag
     2161 gatttcttgc ctttgtagat ttccgccccg atggtggtgt tgtctttgct gatgttatag
     2221 ttggtctcgc tgtggtaaaa gcgcagaaaa tcggtggacc aattgctctg ggtgatgctg
     2281 tattgcagct cgggatgctg gtccaggacg cttttcaaat attcaagctg gtccatttat
     2341 cctcctcgga atgagtttag atccaggtgg tgctgttgtg gcggcgtttg ggattggtgc
     2401 tgcgggagag ggctctgccc gccgcggaag cgaccttgcc tttgttcacc acttttttgc
     2461 cgcgcagcca gacagaatcg atgctgccct tcaaggtgtg ggaactgccg tcgggggcga
     2521 cgaaatcggt gtctttattt gggtcgtaga tcacgaaatc cgcgtccgaa cccgcttcga
     2581 tgcagccctt ggcggggtaa atccccagac gcttggccgc gttttcgctg gtcatcttta
     2641 tcactgtggc cagggggacc ctgcgctttt tccagagttc tgaaagcacc cagaggtagc
     2701 tccagggcag aagctgttcc gacgtgccgt ggaaagcctc gtccaggtct tttgggacgg
     2761 aagcggattc cacgttgttg gaaagcaggt agatcttgtt ggtgcgcagc aggtcaaaaa
     2821 gctcctgttg gtagtcggct aggtccatcc gctgtccgct gtcgatttgg ctgaacagca
     2881 gcatcatgtc ggccacgcac atggcaaagg atatgtcgga gcgcttgtgc acgctgttga
     2941 tgaactcaac tgtgggaaag gcgtttacgc gcgtgatgtg gatggggttt tcctgcatcc
     3001 ggcgcagcag ttttttgatg gcgtcgcttt gcgcgtcgaa gctaaatttc tttgcggtat
     3061 cggtgtcgta tccctgaaag gcgaaggcgg tgtccgattc gtagatatcc atgaagaggt
     3121 ccatgatctc ggtgaagctg agggcctgaa tctcgggatt gggagaagga ttggcgagcg
     3181 cgatgcccac cacgccgttg gcccagagtt cctgcgcagc ttctgcgtga tagggataat
     3241 cagtgatgtc cacgttgccc cagaccgcca tgtccacgaa agaggactgg tcgatctggt
     3301 ctcgcaggcg cttcaaatcg gacaggctga agattggcct gggattcagg aaactcagct
     3361 cggccagggt ggtccagccg ccggagaggg cggctttgct gatccgggcg atactcttct
     3421 tggggtcctg ctccgtgatg atgtggctgt gggggtccac cgcgccgggc aggaggatat
     3481 ttcccttcag gtcgatgact tgggtgattt catcggatat gatctcgtcc gcgctgatct
     3541 tgatgatggt ctagtcaaag agcacattca cccttttcag ggcgaccgag gatgtgggta
     3601 aggcggcatt tgtgaatagc ttcatatagt tccaattatg gggcacggcc acagatgatg
     3661 gctaacacgc aaagcgttac gcatcctgga gccgcgcggt atctgcctgg gggatatggt
     3721 cttgcgtcac gataaaccat actttcccaa actgatgtga tgattttaca actctcaaaa
     3781 tctgtcaagc ttttgcttgc ggcccctgca aaaccaagcc ctccacaaag gctatgacac
     3841 ctctccgatc ccttttgcga cggggttttc agctacagac aacccctttg ccaaagaaaa
     3901 aagacaaaaa agcccttcac cggacttttt tgtcttcgtg tgaatcactg aatggtttat
     3961 gtcatctgag gataagagtc ttcttggtgg atgtgtagcg accctcagtc accgtcacgg
     4021 tgtatttccc ttcaggagtg tagctgccgt c
//
LOCUS       DAOOWR010000142         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22331_length_4042_cov_4.289190, whole genome shotgun sequence.
ACCESSION   DAOOWR010000142 DAOOWR010000000
VERSION     DAOOWR010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22331_length_4042_cov_4.289190"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /gene="rimI"
                     /locus_tag="PLB85_04385"
     CDS             <1..454
                     /gene="rimI"
                     /locus_tag="PLB85_04385"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOU72600.1"
                     /translation="KDVMSEPPVIRPLRETDLPAILGIENLAFSPPWPEEAFRHTEFT
                     QSWVISTDGDLRGYIIYHVVPDEAVIVNFAIAPDFWRHGLGSLLLQHSLDIMQESGIN
                     AVYLDVRRSNLAALKLYNKYGFKPLGVRKNYYSKPREDALVMVRYNNG"
     gene            459..2057
                     /gene="nadB"
                     /locus_tag="PLB85_04390"
     CDS             459..2057
                     /gene="nadB"
                     /locus_tag="PLB85_04390"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOU72601.1"
                     /translation="MRDYDFLVLGSGIAGLVYALQVSRLGRVAVVTKRGLSDCNTNYA
                     QGGIAAVLDEQDTFVNHCEDTFAAGAELGKKQVIRQIITEGPKLIQYLIDLGTDFTLR
                     NESYDNRLENLSLTLEGGHTHRRVAYAADSTGHQIMEVLIERCRENSNIDIFENSIAI
                     DLITQHHVVQTHGFVPGISCWGAYVMDETTNEVHIFRARKTMLATGGASQIYAHNTNP
                     AVSTGDGMAMARLAGARLANMEFVQFHPTAFWSPGGKTFLISEALRGEGAVLRLQDGT
                     EFMKRYHPKGNLAPRDIVSRAIDAEIKKRGEKYCWLDATGIETEKLHNHFPYIDKRLL
                     EQGIDFTRDPIPVAPAAHYFCGGVLSTIDGITDIRNLFAAGEVACTGLHGANRLASNS
                     LLEALVIGWRAGNHPSNQETVVFPELPPWKQMDVFNENEWVVISHNREIIGTIMDGYV
                     GIRRSRRLLKYALSRIENIYNEVNNFYQYNAVRREVVETRNLAIIAIAVIRSALMRRE
                     SRGAHFLIDNPERDDAHYKIDTII"
     gene            2066..2698
                     /gene="tmk"
                     /locus_tag="PLB85_04395"
     CDS             2066..2698
                     /gene="tmk"
                     /locus_tag="PLB85_04395"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU72602.1"
                     /translation="MTGLFITFEGIEGSGKSTQMVLLSKKLTERGLPVLLTREPGGPP
                     IAETIRSLLLDPERREMLPETELLLYCASRAQHTGEWILPALKEGKTVLCDRYFDSTF
                     AYQGAARAQKLDFIRLLTDFATFSTVPHLTFLIDLPVVEGLSRIKYRQLDRLEQEDIS
                     FHERVRNEFLDLARIHSDRFVVLDGSLPIEDIHAKIYSRVLSQLGEPIEQ"
     gene            2688..>4042
                     /locus_tag="PLB85_04400"
     CDS             2688..>4042
                     /locus_tag="PLB85_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU72603.1"
                     /translation="MNNKKQKTALITIAAVWVLSAVMLFTATSVFAQSQNKGTDLYSQ
                     LGLFSEVLSKLKQSYVTELNDEELIKAAIIGMLGSTDPHTNYFTKSEYEDFTTSTKGS
                     FGGLGIQIDKIGDYVTVISPIEGTPAYKMGITAGDKIIRVDDKNIVGVTTDEAIKYMR
                     GEVGTNVTITISRPGVAKPLEFRITRETIKIKSVPYSFKLDNGAGYLRLSQFNENTSQ
                     ELRTALAALEAEGINGLIIDLRWNPGGLLDQAVETVNEFIGANKLVVETKGRMPSANR
                     QYFTRNATRTRDYPIIVLVNEASASASEIFAGSLQDWDKGLVVGKTTFGKGSVQQLIP
                     LINGNGIKITTAYYYIKSGRCIHKKSNDKILLGKEISTSDITAEEELNLKQIYKTVKN
                     REVYGGGGITPDIEIQGEPMTNLAMELRRHNTFFNFAVDYMVEKDHSIDRNFVVDDAL
                     M"
BASE COUNT          998 a         1236 c         1015 g          793 t
ORIGIN      
        1 aaaagatgta atgtccgaac cgcccgtgat ccggccccta cgtgaaactg accttcccgc
       61 catattgggg atcgagaacc tggcgtttag tccgccctgg ccggaagagg ctttccgcca
      121 taccgaattc acgcaaagct gggtgatctc cacagatggg gacttgcgcg gctacatcat
      181 ctaccacgtg gttccggatg aggccgtgat tgtcaatttc gccatcgctc ctgatttctg
      241 gcgccatggc ctgggttccc tgctactgca gcattctctg gacataatgc aggaaagcgg
      301 tatcaacgct gtatatttgg atgtaaggcg ttccaacctt gccgccctca agctgtataa
      361 caaatacggc ttcaaacccc tgggcgtgag aaaaaactac tattccaagc cgcgggagga
      421 cgccttggtt atggtgcggt ataacaatgg atgagagaat gagagattac gattttctgg
      481 tactgggtag cgggattgct ggtctggttt acgcccttca ggtttcccgt ttaggcaggg
      541 tggctgtggt caccaaacgc ggtttgtccg actgcaacac caactacgcc caaggcggca
      601 tcgcagctgt tctggacgag caggacacct ttgtcaacca ttgcgaggac accttcgccg
      661 cgggggccga gctgggcaaa aaacaggtga tccgacaaat catcaccgaa ggtccaaagc
      721 tgatccaata cctcattgac ctgggcacag atttcaccct gcgtaacgaa agctacgaca
      781 accgcctgga aaacctctcc ctcaccctgg aaggcggcca tacacaccgc cgggtggctt
      841 acgcggctga ttccaccggc catcagatca tggaagttct aatcgaacgc tgccgcgaaa
      901 acagcaacat agatatcttt gagaacagca ttgccatcga cctcatcaca cagcaccatg
      961 tggtgcaaac ccacggcttc gtgcccggca tttcctgctg gggagcctat gtgatggacg
     1021 aaaccacaaa cgaggtgcac atcttccgcg cccgcaaaac catgctggcc accggcggcg
     1081 cttcccagat ctacgcccac aacaccaatc cggcggtttc gaccggcgac ggcatggcca
     1141 tggcccgcct ggctggagcg agactggcaa acatggaatt tgtccagttt caccccaccg
     1201 ctttttggag cccgggcggc aaaaccttcc tcatcagcga ggccttgcgc ggggaaggtg
     1261 ctgtcctccg acttcaggac ggcaccgaat tcatgaaaag ataccatccc aagggcaatc
     1321 tggcccccag ggacatagtg tcccgggcca tcgacgctga gatcaagaaa cgaggcgaga
     1381 agtattgctg gctcgacgcc accggcatcg agaccgaaaa actccacaac catttcccct
     1441 acatcgataa gcgccttctg gaacagggga tagatttcac ccgcgacccc atccccgtag
     1501 ccccggccgc ccattatttc tgtggcggag tgctttccac catagatgga ataacggaca
     1561 tccgcaacct ctttgccgcc ggggaggtgg cctgcacggg gctgcatggc gccaacaggc
     1621 tggcctcgaa ctcgttgctg gaagccctcg tgattggctg gcgcgccggc aaccatccct
     1681 caaaccagga aaccgtggtt ttcccggaac tgccgccctg gaagcaaatg gatgtgttca
     1741 atgaaaatga atgggtagtg atctcccaca accgcgaaat catcggcact atcatggacg
     1801 gctatgttgg catccgccgc tcccggcgcc tgctcaaata tgccctttcc cgcatcgaaa
     1861 acatctataa cgaagtgaac aatttttacc agtacaatgc cgtccgccgc gaagtggtgg
     1921 aaacgcgcaa ccttgccatc atcgccatcg ccgtgatccg cagcgcactg atgcgccggg
     1981 aaagccgcgg tgcccatttt ctgattgaca atcccgagcg cgacgacgcc cattacaaga
     2041 tcgacaccat catctaaagg atatcatgac aggacttttc ataacctttg aaggcatcga
     2101 gggaagcggc aaaagcaccc agatggtgct tttgagcaaa aaactcactg aacgagggtt
     2161 gcctgtactg cttacccgcg agcctggagg tcctcccatc gctgaaacca tccgttcttt
     2221 gctgcttgat cccgagcgcc gcgagatgct gccggaaaca gagcttctgc tctattgcgc
     2281 cagccgcgcc cagcacaccg gcgaatggat actgccggct ttgaaagaag gcaaaaccgt
     2341 cctctgcgac cgctacttcg attccacttt tgcctatcag ggtgccgccc gggcccaaaa
     2401 acttgatttc atccgcctgc ttactgactt cgcaactttc agcaccgtgc cccatctcac
     2461 ctttctcatc gacctgcccg ttgtcgaagg gctaagccgc atcaagtacc gccagcttga
     2521 ccgcctcgaa caggaggaca tttctttcca cgaacgggtc cggaacgaat ttctggacct
     2581 cgccaggatc cattccgacc gttttgtggt gctggatggc tccctcccca ttgaagacat
     2641 ccatgccaaa atatattcaa gagttttaag ccagttagga gaacccattg aacaataaga
     2701 aacaaaaaac cgccctgatc accatcgccg ccgtgtgggt cctctccgcg gtgatgcttt
     2761 tcaccgccac cagcgttttt gcccaaagcc aaaacaaggg caccgacctc tattcccagc
     2821 ttggtctctt cagcgaagtg ctaagcaaac tgaagcaaag ctacgttacc gagctcaacg
     2881 acgaagagct gatcaaggcc gcgatcatcg gcatgctggg ttccaccgat ccccatacga
     2941 actacttcac caaaagcgag tatgaagact tcaccacctc caccaaaggc tccttcggcg
     3001 gactaggcat ccaaatcgat aaaatcggcg actacgttac cgtgatttca cccatcgagg
     3061 gcacccccgc ctacaagatg ggcatcaccg ctggtgacaa gatcatccgc gtggacgaca
     3121 agaacatcgt gggcgtaacc accgacgagg caataaaata catgcgcggt gaagtgggca
     3181 cgaacgtgac catcaccatc agccgtcccg gcgtggccaa gcctctggaa ttccgcatca
     3241 cccgcgagac catcaagatc aagagcgtgc cctacagctt taagctcgat aacggcgccg
     3301 gctatctgcg gctaagccag ttcaatgaaa acacatccca ggagctgcgc accgcgctgg
     3361 ccgcccttga ggctgaaggc ataaacggac tcatcatcga tttgcgctgg aatcctggcg
     3421 gactgctcga ccaggcggtg gaaacggtca acgagttcat tggcgccaac aaactggtgg
     3481 tggaaaccaa gggccgcatg ccctcagcca accgccagta ctttaccaga aacgccacca
     3541 ggacacgcga ttatcccatc atcgttctgg tgaacgaggc ctccgccagc gcctccgaaa
     3601 tcttcgctgg ttccctgcag gattgggaca aaggcctcgt ggtgggaaaa accaccttcg
     3661 gcaaaggcag cgtgcagcag ctgattccgc tcatcaacgg caacggcatc aagatcacaa
     3721 ccgcctacta ctatatcaaa tccggccgct gcatccacaa gaagagcaac gacaagatcc
     3781 tgctgggcaa agagatatcc acctcggaca tcaccgccga ggaggaactc aacctcaagc
     3841 agatctataa aacagtgaag aaccgcgaag tttatggcgg aggaggcatc acccccgata
     3901 tcgagatcca gggtgagccc atgaccaatc tcgccatgga actccgccgc cacaacacct
     3961 tcttcaattt cgcggtggat tacatggtgg agaaagacca cagcatcgac cgcaacttcg
     4021 tggtggatga cgcccttatg aa
//
LOCUS       DAOOWR010000143         4034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22399_length_4034_cov_3.065594, whole genome shotgun sequence.
ACCESSION   DAOOWR010000143 DAOOWR010000000
VERSION     DAOOWR010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22399_length_4034_cov_3.065594"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLB85_04405"
     CDS             <1..276
                     /locus_tag="PLB85_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72604.1"
                     /translation="MGKHMCLAATHFTAPALLAGLAHYSIAGLDLSALEGKRFASPSQ
                     RLRALNEAMDYSLRRLDRNQAPPLNAIGVARVLGLPEEILKLTETDE"
     gene            276..1067
                     /locus_tag="PLB85_04410"
     CDS             276..1067
                     /locus_tag="PLB85_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOU72605.1"
                     /translation="MATINIREATEISLEIGRMLLQSGATANRVERMMHKVSEVFGYQ
                     HTETYVTPTGIFISVSDSQTKLNTSIRRIDNRRTDLGKITRISHFVNELEQHYCRDGQ
                     CRLSAEEFLQKLAQIDKAKVYPAWFSVFCGGLTSGCFCLLFGGSWAEFAVAYAVGVLV
                     SLSLRLLGLLQINNFLLNILGAAMVVTFAKLIDIVVPNIKLDNIIIGGIMLLVPGLNF
                     VNAIRDTMSGDLVSGTARAVEAVFIAVAIAAGSGVMLKLWELWGF"
     gene            1069..1521
                     /locus_tag="PLB85_04415"
     CDS             1069..1521
                     /locus_tag="PLB85_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOU72606.1"
                     /translation="MRPFDHLTLMQFTWAALSILGFSIRVNLRGWKILLLALGGGLSW
                     GLYLIFLYYSNSLLFSIFCAVIVVCIYSEIVARLIKVPVSVFVTCAIIPLVPGSSLYY
                     AMQGFIAGNSELASEHTYKVLLISGTIAMAIAVVSSATNLIFRFRNRF"
     gene            1536..2342
                     /locus_tag="PLB85_04420"
     CDS             1536..2342
                     /locus_tag="PLB85_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOU72607.1"
                     /translation="MNKSVKQNGLERNILKMLKGKPLQAAQMLFADPELQAMQEYANI
                     VSIKRLGYNDHGPVHMRKATLNAIKMFKLLHDAGIKMSLEKEGAGTDGDSLMGVLFAS
                     LLHDLGMSVARHNHELISIQLAIPFIERILDAILPPDDHMTKAAIRSIAIEGIFGHMA
                     SQQIHSLEAGLVLIGDGSDMEKGRARIPTILSSEPRIGDIHRTSAGAIQKVVIAKGAE
                     KPISITVEMSASVGFFQVEEVLFPKINKSPVKPYIELYARVIDRELLRYL"
     gene            2539..>4034
                     /locus_tag="PLB85_04425"
     CDS             2539..>4034
                     /locus_tag="PLB85_04425"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72608.1"
                     /translation="MKTSINKLVLLLVFTVLAISAWALVSEYSFTGVTGTYTEITGGT
                     VLGTNANDQECFNAIPLGFTFTYNGADYTQISVMANGFLALGEVVTPNNLPISSTTNA
                     TNNVVSAFSRDLKSRDDGELSYLLSGTAPNQVFTVQWKNYRRSPTSCSNDILNFQIQL
                     HQNGNKVIFAYGSFTAVDAVTAQTVQVGLRGDDNTDFNNRTTTTDWTATTAGIASNAS
                     CRISSTVYPPLGLVFTYSPPQQGTPPNPAQNPVPANNATNVAAGTNLSWVTGGGTVDG
                     YKLYFGTNNPPTNLVNGTIQTGNVYDHPTDLLYSTTYYWKVVPFNAAGGDALDCPLWQ
                     FTTLADPTVTTFPYVQNFDDVTPNALPLGWSTINANGDGYTWETYAGNAQTDPNSVRI
                     RYNQNEAMDDWLVAPPLNLTADTAYIVKFWYRANSATYPEALSVYWGNAGNAAGLTNL
                     LFENTNIVNTDYQMGQGTITPTANGTYYLGFHGHSAMNMFYLYLDSVS"
BASE COUNT          917 a         1271 c         1012 g          834 t
ORIGIN      
        1 atgggaaagc atatgtgcct ggccgccact catttcacgg ctcccgccct cctggctgga
       61 ctggctcatt acagcatcgc gggactggac ctctccgcgc tggagggcaa gcgcttcgcc
      121 agccccagcc agcgtttgcg ggcgcttaat gaggccatgg attacagcct caggcgtttg
      181 gacagaaacc aggcgccgcc cctcaacgcc atcggcgtgg cgagagtcct gggccttccg
      241 gaggagattc taaagctaac ggagacagac gaataatggc gacaatcaat atcagggaag
      301 ccaccgagat cagcctagag atagggcgca tgctgctcca aagcggcgcc accgcaaacc
      361 gcgtggaacg catgatgcac aaggtgagcg aagtttttgg ctaccagcat actgagacat
      421 acgtaacccc caccgggatc ttcatctcgg tctctgattc ccaaaccaaa ctgaacacca
      481 gcatccggcg tatcgacaac cggcgcaccg acttgggcaa gataacccgc atcagccatt
      541 tcgtgaatga actggaacag cattactgcc gtgacggcca gtgccgcctc agcgcggagg
      601 agtttctgca aaaactggcc cagatcgaca aggccaaagt ctatccagcc tggttctctg
      661 tcttctgcgg cggcctcacc agcggctgtt tctgcctgct cttcggcggc agctgggccg
      721 agttcgcagt agcctatgcc gtgggggtgc tggtgagcct ttccctgcgc ctcttggggc
      781 tgctgcagat caacaacttt ttgctcaaca tcctcggagc ggcgatggtg gtaacctttg
      841 ccaagctgat cgacatagtt gtgccgaata tcaagctgga caacatcatc ataggcggaa
      901 tcatgctgct ggtgccgggc ttgaactttg tgaacgccat ccgcgacacc atgagcggcg
      961 acctcgtttc cggaacagcc cgggcagtgg aagcggtatt catagccgtg gcaatcgccg
     1021 ccggcagcgg tgtgatgctg aagctctggg aactctgggg gttttaagat gcggcctttc
     1081 gaccacctaa ccctgatgca gttcacttgg gcggcgctgt ccatcctggg tttttccatc
     1141 cgcgtgaacc tgcggggctg gaagatcctg ctgctggctt tgggcggcgg cttgtcctgg
     1201 ggattgtatc tgatcttcct ctattacagc aattcgctgt tgttttccat tttctgcgcg
     1261 gttattgtgg tatgtatcta ctcggagatc gtagcccgtc tgatcaaggt gccggtttcg
     1321 gtctttgtta cctgcgcgat catccccctg gtgccgggaa gcagtctcta ctatgctatg
     1381 cagggattca tcgcgggaaa ctcggaactg gcttcggagc atacttacaa ggttttgctt
     1441 atctccggga ccatcgccat ggccatcgcc gtggtgagca gcgcgaccaa ccttatcttc
     1501 cggtttagga acagatttta ggagaataag ataacatgaa taaatcagtc aaacagaacg
     1561 gactcgagcg caacatcctc aaaatgctga aaggcaaacc cctgcaggca gcccagatgc
     1621 tcttcgccga tcccgaactc caggccatgc aggaatacgc gaacatcgtt tccatcaaac
     1681 gcctcggcta caacgaccat ggaccagttc acatgcgcaa ggctaccctc aacgccatca
     1741 agatgttcaa gctgctgcac gacgccggca tcaagatgag cctggagaaa gaaggcgccg
     1801 gcacggacgg ggattccctc atgggggttc ttttcgcctc tctgctgcac gacctgggga
     1861 tgtccgtcgc ccgccacaac cacgaactga tctccatcca actggccatt ccctttattg
     1921 aacgcatcct ggacgccatc ctgcctccgg acgatcacat gaccaaagca gccatccgct
     1981 ccattgccat cgaaggcatt ttcggacaca tggcctccca gcagatccac tcgctggaag
     2041 ccgggctggt gctgatcggt gacggcagcg acatggaaaa aggccgcgcc cgcatcccca
     2101 ccatcctttc cagcgaaccg cgcatcggcg acatccaccg cacctcggca ggagccatcc
     2161 agaaagtggt catcgccaaa ggcgcggaaa aaccgatctc gatcacggtg gagatgagcg
     2221 cctcggttgg tttcttccaa gtggaggaag ttttattccc caagatcaac aagagccccg
     2281 tcaagcccta tatagagctg tatgccaggg taatagaccg cgagttgctc aggtatctct
     2341 aaggcgaagg gctcataggc tatctcacgg tgggatatgt atctccaaag aaaaggtggc
     2401 gaaagccaag ctaacttttc cttgacgaat tttatccttt agtttttctg acagtttgag
     2461 cgatgttggg agtttcctgc ttccagcttg gctatcatag tagtgtacta agttagaata
     2521 ccaattcaag gagatgttat gaaaacaagc atcaacaaac ttgtcctcct gctggtcttt
     2581 acggtattgg ccatcagtgc ttgggcactg gtgagcgaat acagcttcac cggcgtcacc
     2641 ggcacctata ccgagatcac cggaggcacc gtcctgggaa ccaacgcgaa cgatcaggaa
     2701 tgtttcaacg cgattcccct gggtttcaca ttcacctaca acggagcaga ctacacgcaa
     2761 attagcgtaa tggccaacgg cttcctggcc cttggcgagg tagttacccc gaacaacctg
     2821 ccgatcagct ccaccacgaa cgccacaaac aacgtggtct ccgctttctc gcgtgacctc
     2881 aagagccgcg acgacggcga gctcagctat ctgctcagcg gaaccgcccc caaccaagtc
     2941 ttcaccgtgc agtggaagaa ctatcgccgc agccctactt catgctcaaa cgacatcctc
     3001 aacttccaga tccagctcca ccaaaacggc aacaaggtca tctttgccta cggttcgttt
     3061 accgccgtgg atgctgtgac cgcccaaacc gtgcaggtgg gcctgcgggg tgatgataac
     3121 actgatttca acaaccgcac caccaccacc gattggactg ccaccaccgc tggcatagcc
     3181 agcaacgcct cctgccgcat cagttcaacg gtctatcccc ctctcggcct ggtcttcact
     3241 tatagccccc cccaacaagg cactcccccc aaccccgccc agaaccccgt tcccgccaac
     3301 aatgccacca acgtggcagc cggcaccaac ctgagctggg tgacaggcgg cggcaccgtt
     3361 gacggataca aactctattt cggcactaac aaccctccca cgaacctggt caacggcacc
     3421 atccagaccg gcaacgttta cgatcatccc accgaccttc tttacagcac cacctactat
     3481 tggaaagtgg tccccttcaa cgcagccggc ggcgatgccc tggattgccc cctgtggcag
     3541 ttcaccaccc tggccgaccc caccgtcacc accttcccct atgtgcaaaa cttcgatgac
     3601 gttaccccca acgccctgcc cctgggctgg tcgaccatca acgccaacgg cgacggctat
     3661 acttgggaaa cctatgccgg aaacgcgcag accgatccca actccgtgcg catccgctac
     3721 aaccagaacg aggctatgga tgactggttg gttgctccgc ccttgaacct caccgcggac
     3781 accgcctaca tcgtcaaatt ctggtatcgc gccaacagcg ccacctatcc ggaagccctt
     3841 tccgtttatt ggggaaatgc cggaaatgcg gccggcctca ccaacctgct cttcgaaaac
     3901 acgaacattg tgaacaccga ttaccagatg ggccagggca ccatcacccc caccgccaat
     3961 ggcacctatt acctgggctt ccacggccac agcgccatga acatgttcta cctctatctg
     4021 gacagcgtct ccat
//
LOCUS       DAOOWR010000144         4032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22422_length_4032_cov_2.924315, whole genome shotgun sequence.
ACCESSION   DAOOWR010000144 DAOOWR010000000
VERSION     DAOOWR010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22422_length_4032_cov_2.924315"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..979)
                     /locus_tag="PLB85_04430"
     CDS             complement(158..979)
                     /locus_tag="PLB85_04430"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOU72609.1"
                     /translation="MAIGKRKIILILIIAVLALAAAFILLRHLLPASLDKPESIAWND
                     STGTFMVSNVGSGKILSLEEEGRYTVFAKGLKAPRGLKVVDELLYVTDDTSIVALDLK
                     TGEQKANIPIKGAKMLNDIEKDHLGKLYITDTKGNCVHVFDPGTNLTIKLSSPLLKAP
                     NGIVYDGPRRQMFIVGLTEESPILALDVNTQSFSVFKNTLYNDLDGIAIDDLGRIYYS
                     SWGEKCLFMIPQEQNRTLIWQSDLQSPADIYYHKPTNEILVPLMEKNQILRFKVD"
     gene            complement(989..2371)
                     /gene="cysS"
                     /locus_tag="PLB85_04435"
     CDS             complement(989..2371)
                     /gene="cysS"
                     /locus_tag="PLB85_04435"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU72610.1"
                     /translation="MLLYNTAKRAKEEFVPITPGQVKMYVCGPTVYNYFHIGNARAFL
                     TFDVLRRYFEFRGYKVDYVQNITDIEDKIIAQSLEEGVSYKEVADKYATAFLDDSAAL
                     GIRRPTHQPRATEVLPEIIAAIAALEKSGHAYEVNGDVYFNTASLPEYGQLSGKKTEE
                     QLPGARVLENLQKRNPADFTLWKAGKPGEPVWNSPWGEGRPGWHTECVVMGQKYLGET
                     FDIHGGGADLIFPHHENELAQALALTGKPLARYWMHNGFLNVDGDKMSKSLNNFFTAR
                     DILARHSAEAIRFFFLSKHYRSPIDFNREIIEESERAVKNFYAALRDIDFKSISSAPD
                     GSYESSEQAFVEAMDDDLNTAKALAVLFDLVRHCKNGQLPRPQREQAALMLVRLGEVL
                     GFFSDLSEKLNTDLPDLSRQLVELLLTYRREARENKDWKLSDRIRDDLASLGVELRDT
                     PSGCDWKIKD"
     gene            complement(2382..>4032)
                     /locus_tag="PLB85_04440"
     CDS             complement(2382..>4032)
                     /locus_tag="PLB85_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72611.1"
                     /translation="RNPQGGWWRNSAVLLADDLYNSSATIETIHSVQTEAAGNNIHPS
                     ILTDKIFAWEYEYDEFQNKPGARDDMMAAINEGRLIWLYTGHGAYDKLGAEDYFNGAA
                     DMGRFNNQDRLPFFVASSCSVCHFDYWGFESLGQKTVMMDNLGAIASFAASRSSSPTS
                     NGPMMNHLLDKLTNGRNSLGQSIMIAKVLNTQSNDNDATYMLMGDPLLNVVPPGRDSL
                     MTVSGQAAQGDTLHARDLAIVSGSFASSPVSGTFEIKAFNTKREYDLGWHTFVTQRGA
                     PLFSGSATVTGGAYQTEFIVPDDVTPGNNGFIVSYVWDPAQKQDYTSYHHPLALSDDA
                     VTAENPDAPEIKLYIGSYDFRPGDTVGTNTTLYARISDSNGINVTGSFGHNIMLILDN
                     SLQPVPVTDYFIYDLDSYTTGLLTFPLTGLSEGPHTLQLIAFDNFNLPAVASVEFNVK
                     ESGELAIERFLIYPNPMQSSTSFTFTLSRDCDLTFDIYSVTGKKIHSFQTSGRQGFNA
                     IPWDGRDKRGDRFANNTYFVKVRASVDGLKAEATECLVIYK"
BASE COUNT          806 a          976 c         1259 g          991 t
ORIGIN      
        1 aggccgccaa caaggttcag gaagtcaaga aatgtcatgg ctggaggcta ctccgattta
       61 gatatttgtg gacatgttac gggcagggga aatcggtcag gtagatttct ctggccccag
      121 agaagataaa aactctggat tggtttcaaa acctggctca atccaccttg aagcgcagga
      181 tctggttctt ttccatcagc ggcacgagga tttcgttggt gggcttatgg taatagatgt
      241 cagccgggga ctggagatca gattgccaga tcagagtgcg gttctgttcc tgggggatca
      301 tgaacaggca tttttcgccc caggaactgt aatagatgcg gcccaggtcg tcgatggcga
      361 tgccgtccaa atcgttgtag agggtgtttt tgaaaacgct gaaagactgg gtgttcacat
      421 ccagggccag gatgggcgat tcttcggtga gaccaacgat gaacatctgg cggcgggggc
      481 cgtcatacac gatgccgttg ggtgctttca gcaggggaga actaagcttg atggtgagat
      541 tggtgccagg atcgaacaca tggacgcaat tgcccttggt atctgtaatg tagagcttgc
      601 caagatggtc cttttcaatg tcgttgagca tcttagcgcc tttgatgggg atgttggctt
      661 tttgttctcc ggtcttgagg tccagggcca cgatgctggt gtcgtcggtt acatatagca
      721 gttcatctac aaccttgagg ccgcgggggg ctttgagtcc cttggcgaaa acggtgtagc
      781 gtccctcttc ttcgagactt aggattttac cgctgccgac gtttgagacc atgaaggttc
      841 cggttgaatc gttccaggcg atgctttccg gcttgtccag gctggcgggc agcagatgcc
      901 gcagcaggat gaaagcggct gccagcgcca ggacggctat gatgaggatg aggattattt
      961 tgcgtttacc gatggccatt tctgcctcct aatcttttat tttccagtcg cagccgcttg
     1021 gggtgtcgcg caactccaca cccaatgaag ccaggtcgtc cctgatccgg tccgaaagtt
     1081 tccaatcctt gttctcgcgg gcttcccggc ggtaagtgag cagcagttcc accagttgtc
     1141 gggaaaggtc tggcaaatcc gtgttcagct tttcggaaag atctgagaag aagcctaaaa
     1201 cctctcccag cctcaccagc atcaacgctg cctgctcccg ctgtggcctt ggcagttgcc
     1261 cgtttttgca gtgacggacg aggtcgaaga gcacggcgag ggctttggcg gtgttcaggt
     1321 cgtcgtccat tgcttccacg aaagcctgtt cggaagattc ataactgcca tcaggggcgg
     1381 agcttatgct cttgaaatcg atgtcgcgaa gggcggcata aaagttcttc accgcgcgtt
     1441 cggattcctc gatgatttcg cggttgaaat ctatcgggga acggtagtgc ttcgagagga
     1501 agaagaagcg aatggcctcg gcgctgtgcc gggccaggat gtcccgcgcg gtgaagaaat
     1561 tattgaggct cttgctcatc ttgtcgccat caacgttcag aaaaccattg tgcatccagt
     1621 atctggccag gggtttgccg gtgagggcga gggcctgggc gagttcattc tcgtgatggg
     1681 ggaaaatgag gtcggcgccg ccgccgtgga tgtcgaaggt ttcgcccaga tacttctggc
     1741 ccatcaccac gcattcggtg tgccagcctg gccgtccttc gccccagggg ctgttccaga
     1801 cgggctctcc gggttttccg gctttccaaa gcgtgaaatc ggccggattg cgtttctgga
     1861 ggttttccag cactctggcg ccgggaagct gttcttctgt ctttttgccg gagagctggc
     1921 catattcggg cagggaggcg gtattgaaat agacgtcgcc gtttacctca taggcgtggc
     1981 cgcttttttc cagagcggcg atggcggcga tgatttccgg aagcacttcc gtggcacggg
     2041 gctgatgggt cggcctgcgg atgccgaggg ctgcgctgtc atccagaaag gccgtggcat
     2101 acttgtccgc gacctctttg taggataccc cctcttcaag tgactgggct atgatcttgt
     2161 cttcgatgtc ggtgatgttt tgcacgtaat caactttgta gccgcggaac tcgaagtagc
     2221 ggcggaggac gtcaaaagtg agaaaggcgc gggcgttgcc gatgtggaag tagttataga
     2281 cagttggtcc gcagacatac attttcacct ggcccggagt gatgggaaca aattcctcct
     2341 tggcgcgctt ggcggtgttg tacaaaagca tgggcaaatc cttatttata gatcaccagg
     2401 cattcggtgg cctcggcctt gagaccgtcg acggaggcgc gtaccttcac gaaataggtg
     2461 ttgttggcga aacggtcacc ccgtttgtcc cggccatccc agggaatggc gttgaaaccc
     2521 tggcgtccgc tggtctggaa gctgtgtatc tttttcccgg tgactgagta gatgtcgaag
     2581 gtgaggtcgc aatcgcggga gagcgtgaaa gtgaagctgg tggagctttg catggggttg
     2641 gggtagatga ggaagcgttc gatggccagt tcgccgcttt ccttcacgtt gaactcaacg
     2701 ctggccacgg cgggtaggtt aaagttgtcg aaagcaatca gttggagggt gtgggggcct
     2761 tcgcttagcc cggtcagggg gaaggtgagt aggccggtgg tgtaggaatc caggtcatag
     2821 atgaaataat ccgtcactgg cacaggctgg agcgagttgt ccaggatcag cattatattg
     2881 tgtccaaaac tacccgtgac gttgatcccg ttgctgtccg aaatcctggc gtaaagcgtg
     2941 gtgttggtgc caacggtgtc gccggggcgg aaatcgtaac tgccaatata gagttttatt
     3001 tccggcgcat cgggattttc ggctgtcacg gcatcgtcac tgagggccag gggatggtgg
     3061 tagctcgtgt agtcttgttt ctgggcggga tcccagacat aggaaactat gaagccattg
     3121 ttgcctgggg tcacatcgtc tggaacgatg aattcggtct ggtaggctcc tccggtcaca
     3181 gtggcgctgc cggaaaaaag aggggcgccg cgttgggtga caaaggtatg ccagcccaag
     3241 tcgtactccc tcttggtatt gaaggctttg atctcaaaag tgccgctgac aggcgaggaa
     3301 gcaaaagatc cggacacgat ggccaggtcc ctggcgtgca gggtgtctcc ctgtgcagcc
     3361 tggccagaca cggtcatcag actatcgcgc cctggcggaa ccacattcag caggggatcc
     3421 cccatcagca tgtaggtggc gtcgttatcg ttgctttgcg tgttgaggac cttggctatc
     3481 atgatggact ggcccaatga gttgcgcccg ttggtgagtt tatcaagcaa atgattcatc
     3541 atggggccgt tgctggtagg cgagctgctg cggctggcag caaatgaggc tatggctccg
     3601 aggttgtcca tcatcacggt tttctgtccc aggctttcaa aaccccagta gtcgaagtgg
     3661 caaaccgagc aggaggatgc tacgaagaag gggagcctgt cctgattgtt gaatcggccc
     3721 atgtccgcag cgccgttgaa atagtcttct gcgcccagtt tatcataagc gccatggccg
     3781 gtgtagagcc agatcagccg gccttcgtta atggcggcca tcatgtcatc cctggccccg
     3841 ggtttgttct gaaactcgtc gtattcgtat tcccaggcaa agatcttgtc ggtgaggatg
     3901 ctgggatgga tattattccc cgctgcctct gtttggacgc tgtggatggt ttcgatagtg
     3961 gcggaactgt tgtagaggtc gtcagccagc agtacggcgg aattccgcca ccagccgccc
     4021 tgggggttgc gt
//
LOCUS       DAOOWR010000145         4014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22566_length_4014_cov_3.281384, whole genome shotgun sequence.
ACCESSION   DAOOWR010000145 DAOOWR010000000
VERSION     DAOOWR010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22566_length_4014_cov_3.281384"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLB85_04445"
     CDS             complement(<1..630)
                     /locus_tag="PLB85_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU72612.1"
                     /translation="MQYRTMPKSKDRLSALGFGLMRLPQTKSGKIDEDKALAMLHFAL
                     DNGVNYFDTAWGYHGGASEPLLGEFVTQTDRSKLFIATKLPCWLVKTRADMDSYLNQQ
                     LERLKTDHIDYYLLHALNARSWKEMLHLGVLDWLDNSRADGRIRHIGFSFHDEYSVFK
                     QIVERYDWDFCQFMLNFLDTQYQAGMKGYKLASAKGLGIIAMEPLRGGKL"
     gene            831..2072
                     /gene="rocD"
                     /locus_tag="PLB85_04450"
     CDS             831..2072
                     /gene="rocD"
                     /locus_tag="PLB85_04450"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOU72613.1"
                     /translation="MSEKLKYGTISSKESMELEEKYGAHNYHPLPVVLAKGEGVFLWD
                     PEGNKYYDFLSAYSAVNQGHCHPKIIQALIDQAKELTLTSRAFFNNKLGDYEKFITEY
                     FGYDMVLPMNTGAEGVETALKLARKWAYEVKGVEKDGAIIIFAANNFHGRTIAIVSAS
                     TDPDCYEGFGPFLPGIVTIPYNSVDALKEYLEKHGKNVAAFIVEPIQGEAGVFVPDEG
                     YLKSCFDLCKEHNVLFIADEIQTGIARTGKLLACDYEDVRPDILILGKALAGGVLPVS
                     AVLADREIMLTIKPGQHGSTFGGFPLACVVAKAALEVVKEENLAARAFELGEKFRKAL
                     KAIKNPMIKLVRGKGLLNAIVVEPKNGYEAWDVCIKLKERGVLCKPTHRHIIRLAPPL
                     VITEEQLDEVAKIIEEVFNSI"
     gene            2124..3011
                     /gene="era"
                     /locus_tag="PLB85_04455"
     CDS             2124..3011
                     /gene="era"
                     /locus_tag="PLB85_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOU72614.1"
                     /translation="MNDRPDFRSGFVAIIGKPNTGKSTLMNRILGEKISITSPKPQTT
                     RYAIKGILNRDDCQIIFIDTPGYLKPRYELQERMQKIWSDAFKDVDLVLFMSDVKRFP
                     TQYDTEVLEHLKDLRTPQIAVFNKLDLEPNVNPDMFLNQLPDSFDKAHFISASTGEGI
                     PELLDSLIQHIPYHAPYYMEDQLSDLPLRFFVQEVIREAIFHHFAQEIPYATAVLIEK
                     YIEKPDRVVIDAVIWLERQSQKPIIIGKKGENLAKIRKYSETQLSAWLETEVQVHLWV
                     KIKPGWRKKSTALKELGFD"
     gene            3126..3914
                     /locus_tag="PLB85_04460"
     CDS             3126..3914
                     /locus_tag="PLB85_04460"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOU72615.1"
                     /translation="MHSNRDEKATLVVDIGNTNITCGIYQRDQMTWFARFHSTCSRTA
                     DEYFALLKPLLPDVSEEYIGSIALASVVPELTRIWQHLFQKYLKASVWELNALSVPGL
                     SYKVPDPSFIGADLIANAYGAWQKYQSSCLILDLGTATKVQLVLAEGCFEGTAIAPGL
                     KTGAAQLFEKAALLSPIEITAPAVLLGTNTRDALLSGIVHSHAFMLENYVRQVKLHYF
                     DYKDIRVILTGGIADLVKPLVPSADIVDKTLTLDGIHLAWKNSH"
BASE COUNT         1007 a         1100 c         1032 g          875 t
ORIGIN      
        1 aagtttccct ccgcgcagag gctccatggc tatgatgccc aagcccttgg ctgaggcgag
       61 cttgtagcct ttcatcccgg cctggtattg ggtgtccaga aagttgagca tgaactggca
      121 gaaatcccag tcgtagcgtt ccactatttg cttgaagacg gaatactcat catggaagga
      181 aaagccgatg tggcgaatcc tgccgtcggc gcgggagttg tcaagccagt ccagcacgcc
      241 gaggtgcagc atctctttcc aggaacgcgc gtttagggcg tggagcaagt aatagtctat
      301 atgatccgtc ttcagccgtt caagttgttg attcaggtag gaatccatgt cagcgcgggt
      361 tttcaccagc cagcagggga gtttggtggc gatgaacaat ttggagcggt cggtttgggt
      421 cacaaattca cccaataggg gttcgctggc gccgccgtga taaccccagg cagtgtcaaa
      481 ataattcact ccgttatcca gcgcgaaatg cagcatggcg agggctttat cttcatctat
      541 cttgccggat ttggtctggg gcaggcgcat caggccgaaa ccgagggccg aaagcctgtc
      601 tttggatttg ggcatggttc tgtattgcat aatcaattcc tttaattgcc atactggggg
      661 cagggaaaat cctgtcaatg gaatttcatg ctgccacata attgccggct ggtttctcgc
      721 gagcctctga actcagatcc gggcttgccg ggaaaagaat gccttgacac aaagatgagt
      781 tataggattt ggcttgtgac aaagaaaaaa ataccttaat ggaggaaaaa atgtccgaga
      841 aactcaaata cggaactatc agctctaaag aatctatgga gcttgaagag aagtacggag
      901 cgcataacta ccatccgctg ccagtggtgc tggccaaggg cgagggagtc tttctctggg
      961 acccggaggg aaacaaatac tacgatttcc tgtcggcata ttcagccgtg aaccagggac
     1021 actgccatcc caagatcatc caagccctca ttgaccaggc caaggaactg accctcacct
     1081 cccgggcttt tttcaacaac aagctgggtg actacgaaaa gtttatcaca gagtattttg
     1141 gctacgatat ggtgctgcca atgaacaccg gtgcggaagg agtggaaact gccctcaagc
     1201 tggcccgcaa atgggcttat gaggtgaagg gcgtggaaaa agacggcgcg atcatcatct
     1261 ttgccgccaa taacttccac ggacgcacca tcgcaatagt ttccgcttcc accgatcccg
     1321 actgctacga aggattcggc cccttcctgc ccggcatcgt cacgattccc tacaactcag
     1381 tggatgccct caaagagtat ctggaaaagc acggcaagaa cgtagccgcc ttcatcgttg
     1441 aaccgataca gggtgaagct ggcgtcttcg ttccggacga aggctacctg aaatcctgct
     1501 ttgacctctg caaagagcac aacgtgctgt tcatcgccga cgagatccag acaggcatcg
     1561 cccgcaccgg caagttgctg gcttgcgatt atgaggatgt gcgccccgac atcctgatcc
     1621 taggcaaggc cctggctggc ggtgtgctgc cggtttcagc cgttttggcc gaccgcgaga
     1681 tcatgctcac catcaagccc gggcagcacg gttccacctt cggcggcttc ccgctcgcct
     1741 gcgttgtggc caaagcagcg ctggaagtgg tgaaagaaga aaacctggcc gcgcgcgcct
     1801 ttgaactggg tgagaaattt cgcaaagccc tgaaagccat caagaacccc atgatcaaac
     1861 tggtgcgggg caaaggcctg ctgaacgcca tcgttgtgga acccaagaat ggctacgagg
     1921 cctgggacgt ttgcataaaa ctgaaggaaa gaggcgtgct ctgcaaaccc acccaccgcc
     1981 acataatccg tctggcacca ccgcttgtca tcaccgaaga acagcttgac gaagtggcga
     2041 agatcattga agaagtgttt aactcaatct aaaacaatgc aactgccaaa gagggcggac
     2101 atcagctccg ccctcaggtt tttatgaacg acagacctga tttccgcagc ggcttcgtgg
     2161 ccatcatcgg caagccaaac acgggcaaat caaccctgat gaaccgcatc ttgggagaaa
     2221 agatatccat cacctccccc aaaccacaaa ccacgcgcta cgccatcaag ggcatcctca
     2281 accgcgacga ctgccagata atcttcatcg acaccccggg ctacctcaaa ccgcgctatg
     2341 agcttcagga aaggatgcag aaaatctgga gcgacgcctt caaggacgtg gacctggtgc
     2401 tgttcatgag cgatgtgaaa aggtttccaa cccaatacga caccgaggtc cttgagcatc
     2461 tgaaggattt gcgcaccccg cagatcgctg tattcaataa gctggacctc gagcccaacg
     2521 tgaacccgga catgtttttg aaccagcttc ccgattcatt cgataaggcc catttcatct
     2581 cagcctccac cggcgaaggc attccggagc tgctggattc gctgattcaa cacataccct
     2641 atcatgcgcc ttattatatg gaggaccagc tatccgatct gccgctacgc tttttcgtgc
     2701 aggaagtgat tcgggaggcc atattccacc attttgccca ggagattccc tacgccacag
     2761 ctgtgctgat cgaaaaatac attgagaagc cggacagggt ggtgattgac gctgtgatct
     2821 ggcttgagcg gcaaagccag aagcccatca tcattggcaa aaagggtgaa aacctggcca
     2881 agatacgcaa atactctgaa acacagctct cggcttggct ggaaaccgaa gtgcaagtgc
     2941 atctttgggt gaaaatcaag ccgggttggc gaaaaaaatc cacagctttg aaggaattgg
     3001 gttttgattg aggaagcagg gggctgaaat tatattgaca caaatcaagc cacgccaatt
     3061 ctagtctccc aagcgaacca gtccttgttt tttgtttttc caaacctatg caacgcgtgg
     3121 agaatatgca ctcaaacaga gacgagaaag ccactctggt agtggacatc ggaaatacga
     3181 acatcacctg tggtatctac caacgcgacc aaatgacttg gttcgccaga ttccacagca
     3241 cttgcagccg caccgcagat gaatattttg cgttgctaaa gccgctgttg ccggacgtca
     3301 gtgaagaata catcggctcg atcgcccttg ccagcgtggt tccggaactg acccgcatct
     3361 ggcagcacct ctttcagaaa tatctgaagg cctcggtttg ggaactaaac gctctcagcg
     3421 ttcccgggct cagctacaag gtgccggatc cctcctttat tggcgccgac cttatcgcca
     3481 atgcctacgg ggcctggcag aaataccaat cttcctgcct catcctggac cttggcacag
     3541 ccaccaaggt gcagttggtc ttagcggaag gctgttttga gggcaccgcc atcgcgccgg
     3601 ggcttaaaac cggcgcggcc cagctctttg aaaaagcggc cctgctatcc ccgatcgaga
     3661 tcacagcgcc ggcagtgctg ctgggaacaa acacccgtga cgccctgctt tcggggatag
     3721 ttcatagcca cgccttcatg ctggaaaact atgtcaggca ggttaaactc cactacttcg
     3781 attacaagga catccgcgtg atccttaccg gcggaattgc tgatttggta aagccgctgg
     3841 tgccctctgc agacatcgtg gacaaaacac ttacccttga cggcatccat ctggcctgga
     3901 agaacagcca ctgatttaca ccgcatgaaa aaacccctcg tcctgatctg cctcctttta
     3961 attgtatccg ggcttttcgc cagcccggag ttctatcccc gcctttggat cgag
//
LOCUS       DAOOWR010000146         4000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22665_length_4000_cov_3.690748, whole genome shotgun sequence.
ACCESSION   DAOOWR010000146 DAOOWR010000000
VERSION     DAOOWR010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22665_length_4000_cov_3.690748"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLB85_04465"
     CDS             complement(<1..770)
                     /locus_tag="PLB85_04465"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOU72616.1"
                     /translation="MHIFKRCLVPCLLLLIGWGSLFAAGETIYDIIVRGNKAVSPQMV
                     TTAITLRTGDLLNLEEVARSIRQLYKMRMFSDVRISTEPYRTGVNLIVDVVENPVLAF
                     IEYEGMKAVKKEKLDELVTVRVGSFWSDFQKHELVSKLTAEYNSKGYNNAKVMLDEQT
                     DAEGKIRVKVSVVEGGRLAINAVTFVGNENIEDSALLKRMKTKPANLFRSGRFEQDKF
                     DQDLIKLADYYKKNGYIDVIVGPHELISTGEKTQELVI"
     gene            954..3842
                     /locus_tag="PLB85_04470"
     CDS             954..3842
                     /locus_tag="PLB85_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOU72617.1"
                     /translation="MKLSPALFAVLILALAVCAWAEPALARKPWAAASGRSFEARADS
                     LTGFDVLSYDLTLNINHTAQHISGNVLATVLAEQNLTSIPYNLVGLSVSQVLVNGNPA
                     SYTHSGGIINISLNIPSGQTFTTQVFYSGNPQLSPNVYHIGMIFNANTVFTISDPDAA
                     RYWWPCYDHPWDKAVVNLSVTLRSDWKVAANGIRASIVDHGDGTATTYWTGSHPMTTY
                     LVCVTCGPYVEINQNAGDLPIQNFVMQNQHNNALTDFANLPDMIDYFSQVFGPYPFEK
                     YGNTVVNMSTYGAMEHQTMTTLGSYIITGTGAHELIIAHELAHQWFGNAVSFLTFADV
                     WLSEGFATYSEHLWTDKTEGWQAACDYVANYYHQYYINWEGSSPQTIYNPSFNNYFAP
                     PSYEKAASVLHMLRLRLGDAQFFQLLQSWFNTYKYGNAITPEFQAMAQNISGQDLSQF
                     FNQWIYSAGIPSLDYSVWNNPVCNSPLKIIAKTVSNTSTPFHIDVPFRFVDGAFTDSL
                     LVKASPEGTLNHFWPELTPNTVLTPNYHNWTLLRGITELSPALAECLPSNSSVLLAWD
                     SFLNDGSLGYNIYRRVSGGSEDWLLLNPEPISSLSFTDFTAQNGVCYEYALSAVDTGG
                     WISKMSSPVSATPVSFSFSHNLLVVDETRDGNGASINPDDQMVDDFYDTVLHPIPHDD
                     WDVATQGLPPLDVLGQYRLLLWHADDFSQNQLIDHQNTLGGYLHGGGKLILSGWKTAS
                     VLSASFLNRFGGIIELIYDNSPSLISAQSSSYATLNIDPEKLIPAWNGMLPYIYTFNK
                     VAYPIYTANMLPTAQGNGACVALRSGDPDTGSVFVLFGFPLYFAQASGVRAFLQEIIP
                     ELIDLTPVDDPVTPPASAALEIWPNPFSASASLRISLPAKGPLSLVLYSLRGQALRRI
                     HAGEKDSGIHSFTLEGRDDSGNPLADGIYLLKMEYAGKTLVRKVSLIK"
BASE COUNT          863 a         1245 c          934 g          958 t
ORIGIN      
        1 ttgatcacaa gttcctgggt tttctctccg gtggagataa gttcgtgcgg tccaactatc
       61 acgtcaatat agccgttctt cttataatag tcagccagtt tgatcaggtc ctggtcaaat
      121 ttatcctgtt cgaagcgccc ggagcgaaag aggttcgcgg gtttggtttt cattcgtttc
      181 agcagggcgc tatcttcaat attctcgttt cccacaaagg tcacggcgtt tatagccagg
      241 cgtccgccct ccaccacgct caccttcacc cttatcttgc cttcagcatc cgtctgctcg
      301 tccagcatca ccttggcgtt gttgtagcct ttgctattgt attccgcggt tagcttgctt
      361 accagttcgt gcttctgaaa gtcgctccag aaagaaccga cgcgcactgt taccagttcg
      421 tccagctttt cctttttaac tgctttcatt ccttcatatt cgatgaaagc cagcaccggg
      481 ttttccacta cgtccacgat taggttcacg cctgtccggt agggctctgt ggagatccgg
      541 acatccgaaa acatccgcat cttgtagagc tgccggatgg aacgggcgac ttcctccaga
      601 ttgagcagat cgcctgtgcg cagggtgatg gcggtggtca ccatctgggg tgagaccgcc
      661 ttgtttccgc gcacgatgat atcgtagatc gtttcgcctg ccgcgaacag gctgccccag
      721 ccaatcaaca gcaacaggca gggcaccagg caccttttga aaatgtgcat ttctcctccc
      781 ttgggaattg acgcctattt ctggcaaggc cgctttttcg taaagagatt tttttgctac
      841 ctcgccatct ctggtcccta tcaggctgtt attgacagaa atctaccgtt ttggaaactt
      901 tattgcatga ctttggggca gtgcccaaac cattttgggt aaggagatca gaaatgaaac
      961 ttagtccggc tttgtttgcc gttttgattc tggccttggc tgtctgcgcc tgggcggagc
     1021 ccgcgctggc ccgcaaaccc tgggctgccg cttccggccg gagttttgaa gcccgcgccg
     1081 attccctcac aggattcgat gtgctatcct acgaccttac cttgaatata aaccacactg
     1141 cccagcacat cagcggaaac gtgctcgcca cagtgctcgc ggagcagaac ctaacttcca
     1201 ttccttacaa tctggtgggg ctcagcgtca gtcaagtgct ggtcaacgga aatcctgcct
     1261 cctacaccca cagcggggga atcataaaca tcagtctgaa tatcccgtca ggccagactt
     1321 tcaccaccca ggtcttctat tcaggcaatc cacagctttc acctaacgtt taccatatcg
     1381 gcatgatctt caatgccaac accgtgttca ctatctccga tcctgatgcg gcccgctatt
     1441 ggtggccctg ttacgatcat ccttgggaca aggccgtcgt caatttgagc gttaccctgc
     1501 gcagcgactg gaaagtggct gccaacggca tccgcgcatc catcgtggac cacggcgacg
     1561 gcaccgccac aacttactgg accggctcac atccaatgac cacttatctg gtttgcgtca
     1621 cctgcggccc ttacgttgag ataaatcaaa acgccggcga cctgcccatc cagaatttcg
     1681 tgatgcaaaa ccaacacaac aatgccctga cagacttcgc caacctgccg gacatgattg
     1741 actatttttc ccaggtcttc ggaccctatc cctttgaaaa atatggcaac acggtggtga
     1801 acatgagcac ctacggcgcc atggaacacc aaaccatgac caccttgggc agttacatca
     1861 tcaccggaac cggtgctcac gaattgataa tcgcccatga gcttgcccac caatggtttg
     1921 gcaacgccgt ttctttcctc actttcgccg atgtctggct ttcagagggc ttcgcaacct
     1981 actcagaaca tctctggacc gataaaaccg agggctggca agccgcctgc gattatgtgg
     2041 ccaactatta ccaccagtat tacataaact gggagggcag cagcccgcaa accatctaca
     2101 atccgtcttt caacaactac ttcgcccctc cttcctacga aaaagcggca tccgtgctcc
     2161 atatgctacg cctcaggctg ggcgacgccc agttcttcca attgctccaa tcctggttca
     2221 acacctataa atacggaaac gccatcaccc cggaattcca ggccatggca cagaacatca
     2281 gcgggcagga cctcagccag tttttcaacc agtggattta cagcgccggc attccctctc
     2341 tggactacag cgtctggaac aaccccgtct gcaattcccc tctgaaaatt atcgccaaaa
     2401 ccgtttccaa cacatcaacc ccattccata tcgatgtgcc tttccgcttt gtcgacggag
     2461 ctttcaccga ttcccttctg gtgaaggcat cccccgaagg gacactaaac cacttctggc
     2521 ctgaattgac acccaacacc gtgctcacgc ccaattacca caattggact ttgctgagag
     2581 gcatcacaga actcagcccc gctttggcgg aatgcctgcc ctccaactct tccgtgcttc
     2641 tggcttggga ttctttcctg aatgacggca gccttggcta taacatatat cggcgtgtgt
     2701 ctggcggttc ggaagactgg ctgctcctca atccggagcc gatctcaagc ctcagcttca
     2761 ctgacttcac cgctcaaaac ggcgtttgct acgaatacgc cttgtcggct gtggacacag
     2821 gcggctggat cagtaaaatg tcctctccgg tctcggccac tccggtgtct ttcagctttt
     2881 cccacaatct cctggtggtg gatgaaaccc gcgacggcaa cggcgccagc atcaaccccg
     2941 acgaccagat ggtggatgac ttttacgaca cggtgctgca ccccatccca cacgacgact
     3001 gggatgtggc cacccagggc cttcccccgc tggatgtttt gggacagtac agactgctgc
     3061 tctggcacgc ggatgacttt tcccaaaacc agttgataga ccatcagaac acactcggcg
     3121 gctacctcca cggcggcgga aagctgatcc tttccggctg gaaaaccgct tccgtgctgt
     3181 cagcaagctt ccttaaccgc ttcggcggaa tcatcgagct catctatgac aattcaccca
     3241 gcctgatcag cgcccagtcc agttcctacg cgacgctcaa catcgacccg gaaaagctga
     3301 tccctgcctg gaacggcatg ttgccataca tttacacttt caacaaggtt gcctacccca
     3361 tctacaccgc gaacatgctt cccacggcac agggcaacgg cgcttgcgtg gctttgcgca
     3421 gcggcgatcc ggatacaggc tctgtgttcg tccttttcgg ttttccgctc tatttcgctc
     3481 aagcttccgg cgtcagggcc tttttgcagg aaatcatccc ggaactcatt gacctcacgc
     3541 cggttgacga tcccgtcacc ccgcccgctt ccgcggctct ggaaatctgg cccaatccgt
     3601 tcagcgcctc cgcttccctg cgaatctcgc tgcctgccaa aggcccgctc agccttgttc
     3661 tatacagcct tcgcggccag gctctgcgcc ggatccatgc cggggaaaag gattcaggca
     3721 tccacagctt tacactcgaa ggccgtgatg acagcggaaa cccgctcgca gacggcatct
     3781 atttgctgaa gatggaatac gccggaaaaa ccctggtgag gaaggtcagc ctgataaaat
     3841 aacccacgga cagaccgaaa ggccctgatg ggagctgaac atgcccgaaa tcgccaatcc
     3901 acgcaggata ttcctgctgc tgttctgcct gctgtccggg ctggcttggg cgcagccgga
     3961 gttctttttc cttaggggac tgcagttcca cagttccggc
//
LOCUS       DAOOWR010000147         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22726_length_3992_cov_3.804420, whole genome shotgun sequence.
ACCESSION   DAOOWR010000147 DAOOWR010000000
VERSION     DAOOWR010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22726_length_3992_cov_3.804420"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLB85_04475"
     CDS             <1..474
                     /locus_tag="PLB85_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72618.1"
                     /translation="YRIYRDGNSISSLSDPGILSFSDTGVSLGTHEYWVRSVFDDPPM
                     LSGPSNTVSVMVSVATDDQLAPVEVGVRVSPNPFHGGTGISIKSEAGARVEFAIYNIR
                     GQLVKNVNLTADNAGKAGFTWDGTDAQGSPVNSGVYQYRMSSAGRFRTGRVVLIR"
     gene            849..1658
                     /locus_tag="PLB85_04480"
     CDS             849..1658
                     /locus_tag="PLB85_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOU72619.1"
                     /translation="MLTIDWKERLRKDTIEYLQKKLPQNDFDFEIIFIAYPERVNGKI
                     PPQVIVHVANSIVKKLGKNHEKYLHFYKHLWDKKGETGRTAFIAIMAKLVQKKPAVYL
                     PMVQKAVNNAHDADLASIFEKVMLPLLRKQPEEYLSYAYDWTRSGKELVRKQAVNLLI
                     KLLKRNPELSGEVVQHFVNQWLQPLDDLASNHVTLLKAVQKLDWELYLDVWRQHDQSR
                     DPQSAEILCASLQSYHPEIEETVENWTKSGNARLKKAAVAAQRILLKKKPA"
     gene            1659..2138
                     /gene="greA"
                     /locus_tag="PLB85_04485"
     CDS             1659..2138
                     /gene="greA"
                     /locus_tag="PLB85_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU72620.1"
                     /translation="MDLSILITPAGMEAIQRRIQKLMGERPEVVKAVMVAREFGDLSE
                     NAEYKAAREKQRQIDGEIDYLRRRCSHLKVIDIDSIPKDKARFGCVCQTRDTANGEEI
                     TFQIVGVDELNFHNREGIQPVSVLSPIGNALLGKKAGELAVVKAPMGERTLEILNIS"
     gene            2159..3577
                     /locus_tag="PLB85_04490"
     CDS             2159..3577
                     /locus_tag="PLB85_04490"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOU72621.1"
                     /translation="MKATKDKAKNTLTYERKNFWKEASAAKIKEAQAFAGPYKEFLNK
                     AKTERETVLWTEALLKANKFQPLTAAKAGKRVYSIFRGKTIAMAVIGSEPVSHGVNMV
                     AAHVDAPRVDLKQNPLYEDKNSDMACMRTHYYGGIKKYQWVSTPLALHGVIVKADGSL
                     LNVCIGEDESDPVFIIPDLLPHLARKEQYTKNLADAIDASRMNLIFSGMQEPGKEEKE
                     ALKAYALKLLNKKYGIVEQDFISAELQLVPATPARDAGFDASMVVGYGQDDRICAYAG
                     LQAMLANLDAKPKRTMVVYFSDKEEVGSQGNTGAQSVFIQDFVASLLRHNGENDSSSN
                     LRQTFMNSMILSGDVTAAVDPNYPNVHERQNAVLFNHGVGISKFTGSNGKYSCNDANA
                     EFTAKVIRIFDTAGVFWQMGELGKVDEGGGGTIAYILANLGAEVIDCGTGLMGMHSLY
                     ELCSKADLYSTFAGYKAFLIAK"
     gene            3607..>3992
                     /locus_tag="PLB85_04495"
     CDS             3607..>3992
                     /locus_tag="PLB85_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72622.1"
                     /translation="MRKTLFALLIVMLALSACVSNKTFKAQQAKVQIMEARQNNQDAE
                     LEMARKDIINNREKLDQLIIRLNSVDEQLQVLEPMQDEIVNSATALVNLQGEVAAISG
                     QLNEAIAANAKLEQKLTALTTDTNET"
BASE COUNT         1062 a         1145 c         1045 g          740 t
ORIGIN      
        1 taccgcattt accgtgacgg caatagcatc tccagcctct cagatccggg catcctctct
       61 ttcagcgaca ccggggtttc cctcggcacc catgaatact gggtccgctc ggtctttgac
      121 gatcccccga tgctttccgg cccgtccaac actgtctccg tgatggtcag cgtggccaca
      181 gacgaccagt tggcccccgt ggaagttggg gtgagagttt ccccaaatcc cttccacggc
      241 ggcaccggga taagcatcaa aagcgaagcc ggggccagag tggaatttgc catttacaac
      301 atccgcggcc agttggtgaa aaacgtgaac ctcaccgcgg acaatgcagg aaaggcaggc
      361 ttcacctggg acggaactga tgcacaaggc agccctgtga acagcggtgt ttaccaatac
      421 cggatgtcca gcgccggcag gttcaggacc ggccgcgtcg tccttatcag gtaagtcttg
      481 cgggccgctt tctgattgag cacccacttg attctttaga tagaaatgtt tagccgccaa
      541 gaggccctgc ctgtcaggcc ctgagcgctt tcaaccagcc ctctcccccg cctcccgcaa
      601 gatactcgca tcaaatgcgg gcattatgcg ggaggcgtat tagatccact ggacagggcc
      661 ttaacggcca gaccgcctcg cttcattcct ccaaagggtc actaaccaat acacagtccc
      721 accatcgtgt tcagcatccc gggtaaaacc acctgatttg tgtgagaacc gaacgatttt
      781 cccttgacat tttcctgccc tgattgcctt tggcattttg agaagaaaac cattgaaaag
      841 gagaaaagat gcttaccatc gactggaagg aaaggctcag aaaggacacg atcgagtatc
      901 tgcagaagaa gctgccccaa aacgactttg actttgaaat aatcttcatc gcctatcccg
      961 aacgggtcaa tggaaagata cctccccaag tgattgtgca cgtggccaat agcattgtta
     1021 agaagctggg caagaatcac gagaaatacc tgcattttta caaacacctt tgggataaaa
     1081 aaggcgaaac cggcagaacc gcgttcatcg ccatcatggc caagctggtg cagaaaaaac
     1141 cggcggtgta cctgcccatg gtgcaaaaag ccgtgaacaa cgcccatgat gccgatctgg
     1201 catcgatttt tgaaaaagtg atgctgccgc tgctgcgcaa gcaaccggaa gaatacctca
     1261 gttatgccta cgactggacc cgcagcggca aggaactggt gcgcaaacaa gccgtgaacc
     1321 ttttgataaa gctattgaaa aggaacccgg aactgagcgg agaggtggtg cagcacttcg
     1381 ttaaccagtg gctgcaacct ctcgacgatc ttgcctccaa ccacgtgacc ttgctcaaag
     1441 cggttcaaaa gctggactgg gagctttatc tggacgtctg gcgccaacat gaccagagcc
     1501 gcgatcccca aagcgctgaa atcctctgcg cctccctgca aagctatcat ccggaaatcg
     1561 aggagaccgt cgagaactgg accaaaagcg gaaacgcccg gctcaaaaag gccgccgtgg
     1621 ccgcccaacg catcctgcta aaaaagaagc ctgcctgaat ggacctcagc attctgataa
     1681 cccccgctgg catggaagca attcagcgcc gcatccaaaa gctgatgggt gaacgccccg
     1741 aagtggtgaa ggccgtgatg gtcgcccgtg aattcggcga tttgagcgag aacgcagaat
     1801 acaaggctgc gcgtgaaaaa cagcgccaaa tcgacggtga aatcgactat ttgcgccgtc
     1861 gctgttcgca cctgaaagtt atcgacatcg actccatccc aaaagacaaa gcccgcttcg
     1921 gctgcgtttg ccaaacccgc gacaccgcca acggcgagga aatcaccttc cagatcgtgg
     1981 gtgtggatga gcttaacttc cacaaccggg aaggcatcca gccggtttcc gtgctgtcgc
     2041 ccatcggaaa cgccctgctt ggcaaaaagg ccggcgaact cgccgtggtg aaagcaccta
     2101 tgggggaaag gacccttgaa attctgaaca tatcctgata agcataaacg gagaataaat
     2161 gaaagcaacc aaagacaaag ccaaaaacac tctaacgtac gagcggaaaa acttctggaa
     2221 agaagcttcc gccgcgaaaa tcaaagaagc ccaggcattc gcagggcctt acaaggaatt
     2281 tctgaacaaa gccaagaccg agcgcgaaac cgtgctctgg acggaagccc tgctcaaggc
     2341 gaacaagttt caacccctga ccgccgccaa ggccggaaaa cgcgtttatt ccatctttcg
     2401 cggcaagacc atcgccatgg ccgtgatcgg cagcgagccc gttagccacg gagtgaacat
     2461 ggttgccgcg cacgttgacg cacccagggt ggacctcaag caaaacccgc tctacgagga
     2521 taaaaacagc gacatggcct gcatgcgcac ccactattac ggcggaatca agaaatacca
     2581 gtgggtatcc acaccgctgg cgctgcacgg cgtcatcgtg aaagccgatg gctcgttgct
     2641 gaatgtctgc atcggcgaag acgaaagcga cccggtcttt atcattccgg acctgctgcc
     2701 ccatctggcc cgcaaggaac agtacaccaa gaatctggca gacgccatcg acgcctcgag
     2761 gatgaacctg atcttcagcg gcatgcagga accgggcaaa gaggaaaaag aagccctcaa
     2821 agcctatgcc ctcaagcttt tgaataagaa gtacggaatt gtcgagcagg acttcatctc
     2881 cgccgaactg cagttggtgc cggccactcc agcccgggac gctggctttg acgcctcgat
     2941 ggtggtgggc tatggccagg acgaccgcat ctgcgcctac gccgggctgc aggccatgct
     3001 ggccaatctg gacgccaaac ccaaacgcac catggtggtc tatttctctg ataaggagga
     3061 agttgggagc cagggcaaca ccggagccca gtccgtcttc atccaggatt tcgtagcctc
     3121 ccttctccgg cacaacggcg aaaatgacag cagttcgaac ctgcgccaga cctttatgaa
     3181 ctccatgatc ctctccgggg acgtgaccgc ggccgtggat cccaactatc caaacgtgca
     3241 cgagcgccag aacgctgtgc ttttcaacca cggcgtcggc atctccaaat tcaccggcag
     3301 taacggcaaa tacagctgca acgacgccaa cgctgaattc accgccaaag ttatccgcat
     3361 cttcgacacg gccggggttt tctggcagat gggcgaactg ggcaaggtgg atgaaggcgg
     3421 tggcggcacc atcgcttaca ttttggccaa cctgggcgca gaagtgatag actgcggcac
     3481 aggcctgatg ggcatgcatt cgctttacga gctgtgcagc aaagccgacc tgtattccac
     3541 tttcgcagga tacaaagcat ttctgattgc taaatagatc aactatctaa aagtaaggag
     3601 gaaaagatga gaaaaacgtt attcgcgctg ctgatcgtga tgctggcatt gtcagcctgc
     3661 gtaagcaaca agactttcaa ggcgcaacaa gccaaggtcc agatcatgga ggcccggcag
     3721 aacaatcagg acgccgaact ggaaatggcg cgcaaagata tcatcaataa tagggagaag
     3781 ctcgaccaac tgatcatcag gctaaacagc gtggacgagc agttgcaggt gctggaaccg
     3841 atgcaggacg aaatcgtgaa ctcagccacg gctctggtga acctccaggg tgaagtggca
     3901 gccatcagcg gccagctaaa cgaggccatc gcggccaacg ccaagctgga gcagaaactc
     3961 accgccctca ccaccgatac caacgagacc tt
//
LOCUS       DAOOWR010000148         3970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22876_length_3970_cov_4.288378, whole genome shotgun sequence.
ACCESSION   DAOOWR010000148 DAOOWR010000000
VERSION     DAOOWR010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_3970_cov_4.288378"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLB85_04500"
     CDS             complement(<1..335)
                     /locus_tag="PLB85_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU72623.1"
                     /translation="MLTGIQIRNYLFVPEQKLSFGPGMTVLSGETGAGKSILVGSVSL
                     IFADPAPALEAFDPQQPIYLEASFDLSHNFEVRDWLAEQGYEPEDELVLAREVSTAGK
                     SSYFLNGRKV"
     gene            complement(336..1193)
                     /locus_tag="PLB85_04505"
     CDS             complement(336..1193)
                     /locus_tag="PLB85_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU72624.1"
                     /translation="MKNFAIYIKPSFADKQAIFAKLEALAAEHGVRFHGIREQRHLMP
                     KGFSVLDPEGKTAKKLDCILVFGGDGTILRAKDLALSSGAPILGINMGWLGFLSETTL
                     GELNKSICDLLKGKYRLLPRMTVQCQLRRQGEIIFKGLALNDAVIHKADTPRLIGVRI
                     FSGGRFVFDTRCDGVVASTPTGSTAYSLAAGGPILAPELRAIVLTPLNPHLLTIRPMV
                     FSSRDRILMRVHGLEEPAALQLDGVNCSPLQENDEVLITAAKQQVQFVKLSNRTFYQI
                     LRRKMHLGK"
     gene            complement(1190..2203)
                     /gene="rsgA"
                     /locus_tag="PLB85_04510"
     CDS             complement(1190..2203)
                     /gene="rsgA"
                     /locus_tag="PLB85_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU72625.1"
                     /translation="MRVPNLDALDDVREVAHYKSQRTAEKQNQTFKQSSELIPGRILE
                     VRTNYNYLVEAEGKFYEATLSGRLKQFSYSSRALSAVGDRVELDISGDPHFRIENILP
                     RVNTLSRYASGNFQKEIIVAANIDQVVITASWRMPLIKPGLIDRYICAATLENIQPVI
                     VINKIDLCEDREELADTVDYYRESGYPLLYTSVVSGEGMEDLRAILIDRDSVFSGQSG
                     TGKSSLINWLEPGLELRTAEVSDFNEKGKHTTTQAILFPWSFGGHLLDTPGIKTVNLH
                     RSAISEIPRVFPGFAKLAPNCFFRDCTHTHEDDCAVLDAQEAGKIPLERYESYLRILE
                     SLE"
     gene            complement(2265..>3970)
                     /locus_tag="PLB85_04515"
     CDS             complement(2265..>3970)
                     /locus_tag="PLB85_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HOU72626.1"
                     /translation="YAWTMYTHWIHGGDPLEALGFEESMKELRRGLSEPYYEKLIEQT
                     ILENPHASQVIYTPVPGLGNQWDSKLKEELAAVKAKMTPQEIEELIKFNHAFLDWQNE
                     EPVPEELEKIPMLSLDDIDPEAERYPTEIEKSREFTLLKHELNTNGVLYLSAYFDLSH
                     ASEANLPWISLYTMLAGQVDSRDQGYAELSNEINIHTGGISLGLLLRSNYQDPDEVLP
                     KLLLNGKAVRNKTGKLIELAAELALRPVFTDRDRLKQIIRETKSKMESMLFYNGHQIA
                     VNRMLAPFSQAHRYSDMISGLEYYHFLNNLEKSLDTEIDKIVAELGWVRDTFFTRNNL
                     LISLTADEAGIAEAFGQLKPFVESLSSEAPSPVERRFQPKDLNEAILAPVQVQYCVKG
                     GNFFRNGYSYSGKLRVLNNILSTEFLHREIREKGGAYGAWSNFSPYGSMYFCSYRDPN
                     LQETLETYDKVPEFIRNFNCSRREMVKYIIGDISSQDYPLTPERKAAQANDDYITGFS
                     HEDRQQIRDEVLSTKVEDIRAFADLVEATMACNHYCVFGNEAKLKEAEKLFDRLTPVF
                     S"
BASE COUNT          757 a         1079 c         1170 g          964 t
ORIGIN      
        1 actttgcgcc cgttcaggaa atagctggat ttccccgcgg tactcacctc ccgggccagc
       61 accagttcat cctccggttc gtagccctgc tccgccagcc agtcccttac ttcgaagtta
      121 tgggaaagat caaaactggc ttccagatag atgggctgct gggggtcgaa ggcttccagc
      181 gcgggtgccg gatcggcaaa gatgagggac acggagccca ccaatatgga ttttcccgcc
      241 cccgtctccc cgctcaggac ggtcatgccc gggccgaaac tcagcttctg ctcgggcacg
      301 aaaaggtagt tgcggatttg gatccctgtg agcatttact tacccaggtg catcttgcgc
      361 cgcagaatct ggtaaaaggt gcggttggaa agcttcacga actgcacctg ctgtttggct
      421 gcggtgatca gaacctcatc gttttcctgc agcgggctgc agttcactcc gtcaagctgc
      481 agcgcggcag gttcctccaa gccgtgaacg cgcatgagga ttctgtcgcg ggaggaaaag
      541 accattggcc ggatggtgag cagatgagga ttaagcggag tgagcacaat ggcgcgcagt
      601 tccggggcga gaatgggccc tccggcggcc agtgaatagg ctgtggaacc ggttggagtg
      661 ctggccacaa cgccatcgca gcgtgtgtcg aagacgaagc gtccgccgct gaagatgcgc
      721 accccgatca ggcgcggggt gtctgccttg tggataaccg cgtcgtttag ggcaaggcct
      781 ttgaagatta tctccccctg ccgccgcaac tggcattgga ccgtcattct gggcaatagc
      841 cggtatttcc ccttcagcag gtcacagatg gatttgttca gctcgcctaa agtggtttcg
      901 gaaagaaagc ctagccagcc catattgatg cccaggatcg gggctccgga actgagggcc
      961 aggtctttgg cccgcaggat agtgccgtcg ccgccaaaga caaggatgca gtccagcttc
     1021 ttcgcggtct tcccttcagg gtccaaaacg ctgaatcctt ttggcattaa gtgccgctgc
     1081 tcgcgaatgc cgtggaagcg gaccccgtgt tcggcagcca gggcttctag cttggcgaat
     1141 atggcctgtt tatccgcgaa agagggtttg atgtagatgg cgaagttctt cattccagcg
     1201 attccagtat cctcagatac gattcatagc gctccaacgg gattttgccc gcctcctggg
     1261 catccagaac ggcgcagtca tcctcatggg tgtgggtgca atcacggaaa aagcagttcg
     1321 gagccagttt cgcgaaaccc ggaaacactc tcggaatctc gcttatcgcg ctgcggtgca
     1381 ggttcactgt cttgattccg ggggtgtcga gcaagtgtcc gccgaaactc cagggaaaca
     1441 ggatcgcctg ggtggtggtg tgcttccctt tttcattgaa atcgctcacc tcggccgtgc
     1501 gcagctccaa ccccggttcc agccagttga tcagcgagct tttgcccgtg ccggattgcc
     1561 cggaaaacac gctgtccctg tcgatcagga tggcgcgcaa atcctccatc ccttcgccgc
     1621 tcactacgga ggtgtagagc agcggatagc ctgattcgcg gtagtaatca accgtgtcag
     1681 ccagttcctc gcggtcttcg cagaggtcga tcttgttgat cacgattacc ggctggatgt
     1741 tctccagggt ggcggcgcag atatagcggt cgatcaggcc cggcttgatc agtggcattc
     1801 gccaggaagc tgtgatcacc acctggtcga tgttggcggc tacaatgatc tctttctgga
     1861 agttgccgct ggcgtagcgg gaaagggtgt tcacccgggg caggatgttt tcgatgcgga
     1921 aatggggatc gcctgagatg tccagctcaa cgcggtcgcc cacagcagaa agcgctctgc
     1981 tggagtagga gaactgcttc agccggccgg agagggtggc ttcgtagaac tttccttccg
     2041 cctcaaccag atagttgtag ttggtcctca cttccagaat tcgcccaggt atcagttccg
     2101 agctttgctt gaatgtctgg ttctgctttt ccgccgtgcg ctgagatttg taatgtgcca
     2161 cctcacgcac gtcgtccagt gcgtcaaggt tcggaacacg cacgtttctc agccgtctcc
     2221 cggcgtgggg ccgctgtttt ttcctgtcct tttgcttcat cgcctcagct gaaaaccggt
     2281 gtcagcctgt caaacagctt ttccgcctct ttcagtttcg cttcgttgcc aaacacgcag
     2341 taatggttgc aggccatggt cgcctccacc aggtcggcaa aggcgcggat gtcctctacc
     2401 ttggtggaaa gcacctcatc acgtatctgc tggcggtctt cgtggctgaa accggtaata
     2461 taatcgtcat tggcttgggc agcttttctt tctggggtca ggggataatc ctggctggag
     2521 atgtcgccga tgatgtattt taccatctca cggcgactgc agttgaaatt gcggatgaat
     2581 tccggcacct tgtcgtaggt ttccagggtt tcctgcagat tgggatcgcg ataggagcag
     2641 aaatacatgc tgccgtaggg cgagaaattt gaccaggcgc cataagcgcc gcccttttcc
     2701 cgaatctcgc ggtgcaggaa ttcggtggac aggatgttgt tcagcaccct caacttgccg
     2761 gagtaggaat agccgttgcg gaagaagttt ccgcctttca cgcaatactg cacctgcacc
     2821 ggagccagga tcgcctcgtt gaggtctttg ggctggaagc gccgttccac aggggagggt
     2881 gcttcggagg aaaggctttc cacaaatggc ttcaactggc cgaaggcttc cgcaatgccc
     2941 gcttcgtctg cggttaggct gatcagcagg ttgttgcggg tgaagaaggt atcgcgcacc
     3001 cagccaagct cggcaacgat cttgtcgatc tccgtatcca ggctcttttc caggttgttg
     3061 aggaaatggt aatattccaa tcctgaaatc atgtcgctgt agcggtgggc ctggctgaac
     3121 ggagccagca ttcggtttac cgcaatctgg tgcccgttgt agaacagcat ggattccatc
     3181 ttggatttgg tttcgcggat gatctgcttc agccggtcac ggtccgtgaa aacgggacgc
     3241 agggcaagct cagccgccaa ttcaatcagt ttgccggtct tattgcgcac cgcctttccg
     3301 ttgagcagca gttttggcag cacctcgtcc ggatcctggt aattgctgcg gagcagcagg
     3361 cccaggctga tgccgccggt gtggatgttg atttcgttgg aaagctccgc gtagccttgg
     3421 tcgcggctgt ccacctgtcc cgccagcatg gtgtagagcg aaatccaggg caggtttgcc
     3481 tcactggcat ggctgaggtc aaaataggct gagaggtaca gcacgccatt ggtgttaagc
     3541 tcatgtttca gcagggtgaa ttctctggat ttctcgattt cggtggggta gcgctccgcc
     3601 tcgggatcaa tgtcatccag gcttaacatg gggatttttt ccagttcttc cgggaccggc
     3661 tcttcatttt gccagtcgag gaatgcgtgg ttgaatttga tcagttcctc tatctcttgg
     3721 ggggtcattt tggccttgac cgcggccagt tcttccttga gtttgctgtc ccactggttg
     3781 cccaggccgg gcacaggggt atagatgacc tggctggcgt gtgggttttc gaggatggtc
     3841 tgctcgatca gcttttcata gtaaggctcg ctgaggccgc ggcgcagttc tttcatcgat
     3901 tcttcaaaac ccagcgcttc cagcggatcg ccgccgtgga tccagtgcgt atacatcgtc
     3961 caggcgtaat
//
LOCUS       DAOOWR010000149         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22891_length_3968_cov_3.353693, whole genome shotgun sequence.
ACCESSION   DAOOWR010000149 DAOOWR010000000
VERSION     DAOOWR010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22891_length_3968_cov_3.353693"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLB85_04520"
     CDS             <1..568
                     /locus_tag="PLB85_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72627.1"
                     /translation="PHGKPPSPELVSQGMVLSNLRLELKQPDYPDLLWYIVKPRPWVE
                     GSQAIFAEPEFGQKTFFVYVPVSGPGWLEIEAVNPWGAISDSLYVAYDYEEPGLELLV
                     APNPVRSRSLASAKWYLPEGSTVMVELKLFNSRGQKVLSRSFEQTEPGEGIWLLSAEP
                     DFRKLGRGIFILSLKVGKRSCLVKLAIL"
     gene            731..1468
                     /locus_tag="PLB85_04525"
     CDS             731..1468
                     /locus_tag="PLB85_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU72628.1"
                     /translation="MNTTAPEISKNRIRELSKLQQKKQRLSKGKVVVEGLRTLKQLRD
                     WGIKPLEQYCLADSSPVWTDVPSFVLHDWELGKICDSEHPQDVAALFDLPRERKIEFK
                     LAFYLDGIKDPGNLGTIFRLCVAFGIDALLLSPDCVEVSSPKVVRASLGSVFKVPFQI
                     LDAEQLCSIQAELVLTDASSGEALRSFVPNADRATMIALGSEAHGLSEELRALADRTL
                     RIEMGEWMESLNVAVSAGIIAHHLFGT"
     gene            1543..2106
                     /locus_tag="PLB85_04530"
     CDS             1543..2106
                     /locus_tag="PLB85_04530"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU72629.1"
                     /translation="MVNIINQKTGWIEVICGSMFSGKTEELIRRIRRAEYARQGILVF
                     KPTIDNRYDATSIVSHSQMQAPSVPISQPEEIHQYLKEEIKIVAIDEAQFFDESIVDV
                     CNDLADKGYRVIVAGLDQDYKGQPFGSMPQLMAVAEYVTKILAICVKCGNPANRTQRT
                     VQKGEQILVGSTEAYEARCRNCHEVLE"
     gene            2103..2351
                     /locus_tag="PLB85_04535"
     CDS             2103..2351
                     /locus_tag="PLB85_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOU72630.1"
                     /translation="MRIASSFAGVIAALLDLYSLLIIIRALMSWFMQDQSSGIYRWLV
                     KITEPVLSPIRKVLPRMRVDLSPVVAVILIGIVIRIIK"
     gene            2374..2955
                     /locus_tag="PLB85_04540"
     CDS             2374..2955
                     /locus_tag="PLB85_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOU72631.1"
                     /translation="MSLFPTDIRHQEFSSSLFGYSKKEVKEYLEELAAEVEEYQNRQE
                     KELQRRELAQLEVRQEAVQAGNAVEELKRREELISRTLVFAEKTKADIISNARKEAEN
                     IIHEAELRAKRAIQEAKQYLGMLEQQYIQLKEQKRQFLMQFRSELKTFQDRIDKDPLL
                     SKPPEHILDSDFKQIKEEIAPNAKAPVEDNQLS"
     gene            2952..3767
                     /locus_tag="PLB85_04545"
     CDS             2952..3767
                     /locus_tag="PLB85_04545"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU72632.1"
                     /translation="MSAAYRQTAEWLRQRLPEVPKVAMILGTGLNGIAEKGRILVQIP
                     YTEIPGFASSTAPSHKGNLILGELGGQKVLYLQGRFHHYEGHPFSTVVFPTRVLACLG
                     IETLIVTNASGSLREELSPGSIVMLKDHINFMGGNPIIGENDESLGERFPSLNHPYDP
                     EYREACAAIAARAGIPMREGVYIAVTGPSLETRAECAAFASWGADLVGMSTVPEVIAA
                     RHAGMKVLAFSVVTNYSNLLHDQAHSQEEIRANADKASAWLISIITEFVTGLK"
BASE COUNT          991 a         1055 c         1135 g          787 t
ORIGIN      
        1 gccacacgga aaaccgccct ctccggagct ggtgagccag ggcatggtgc tatccaacct
       61 gcggctggag ctgaaacagc cggactaccc tgatttgctt tggtatattg tgaagccccg
      121 accctgggtg gaaggcagcc aggccatctt cgctgaaccg gagttcgggc aaaaaacctt
      181 cttcgtctat gttccggtca gcggtccggg ctggctggag atcgaagccg tgaatccctg
      241 gggcgcgata tccgattcac tgtatgtggc ttatgattac gaggaaccag gcctggaact
      301 gctggtggcg ccaaaccccg tgcggagcag gtctctggcc tccgcgaagt ggtatctgcc
      361 ggaggggagc accgtaatgg tggagctgaa gcttttcaac tcacgcggac aaaaggtttt
      421 gagccggagt ttcgagcaaa cggaacccgg agaaggaatc tggctgctct cggcggaacc
      481 tgatttccgc aaactgggca gggggatctt catcctcagc ttaaaagttg gtaaaaggtc
      541 atgtctcgtg aaactcgcaa tcctataaga aatgccgttt tgcgcgcttt gtggataaac
      601 tgtggataac tttgtggacg gacggcgaca gcttgataac aggttgcctg acaataagat
      661 agaaaatgtg gataatccac tccttttgtg gataagtgcg tagctaactg agaatgagga
      721 gattggagat ttgaatacga cagcgccgga aatcagtaaa aacagaattc gggaactgag
      781 caaattgcag caaaagaaac agcgcctgag caagggcaaa gtggtggtgg aggggctgcg
      841 cacgctcaag cagttacggg attggggcat caagccgctg gagcagtatt gtctggcgga
      901 ttccagcccc gtctggacag acgttccgag ctttgtgctg catgactggg aacttggcaa
      961 gatctgtgac agcgagcatc cgcaggatgt ggcggcgctt tttgacctgc cgcgggagcg
     1021 taagattgaa ttcaagctgg ccttttacct cgacggaatc aaggacccgg gcaacctggg
     1081 caccatcttc aggctctgcg tcgctttcgg gatagacgct ttgctgctgt cgccagattg
     1141 cgtggaagtg tcctcgccaa aagtggtcag ggcctcccta ggcagcgtgt tcaaagttcc
     1201 gttccaaatc ctggacgcgg agcaactgtg ttctatccaa gcggaactgg tgctcaccga
     1261 cgcttcttcc ggcgaggctt tgcgttcttt tgtccccaat gccgacaggg cgaccatgat
     1321 cgcgctcggt tccgaggcac acggcctctc agaagagctc agggctttgg cggacaggac
     1381 cttgcgcatc gagatgggag agtggatgga gtcgctgaac gtggcggtgt ccgcgggaat
     1441 catagcgcac catcttttcg gaacctgaga cactttagag attgacaaaa acccggctcc
     1501 ggcattactg acatcttcgt caatttatgt atttaaggag tcatggtgaa catcattaat
     1561 cagaagaccg gctggatcga agttatctgc ggaagcatgt tcagcggaaa aacggaggaa
     1621 ctgatccgcc gcatccgccg ggccgaatac gcgcggcagg gcatattggt gttcaagccc
     1681 accatagaca accgctatga cgccaccagc atcgtttccc attcccagat gcaggcgccc
     1741 tccgtgccta tcagccagcc ggaggaaatt caccagtatc tgaaggaaga gataaagatt
     1801 gtggccatcg acgaagccca gtttttcgac gaatctatcg tggatgtttg caatgatctg
     1861 gcggacaagg gttaccgggt gatcgtggct ggactggacc aggattacaa aggccagccc
     1921 ttcggcagca tgccgcaact tatggcggtg gcggaatacg tgaccaaaat tctggctatc
     1981 tgcgttaaat gcggcaatcc ggccaaccgc acccaacgca cggtgcaaaa aggggaacag
     2041 atactggtag gcagcaccga ggcttacgaa gcgcgctgtc gcaactgcca cgaggtgctg
     2101 gaatgagaat cgcctccagt ttcgctggcg tgatcgcggc tctgcttgac ctctactcgc
     2161 ttttgatcat catccgcgcc ctgatgagct ggttcatgca agaccagagc agcggcatct
     2221 acaggtggct ggtgaagatc acggaacctg ttctgtctcc catccgcaag gtgctgcccc
     2281 gcatgagagt ggacctttcg ccggtggtgg ctgtcatcct gatcggcatc gtgatccgca
     2341 tcataaaata gaatttcaat cctgggagga ttcatgtcat tgtttcccac agacatacgc
     2401 catcaggagt tttcgagctc cctgttcggc tatagcaaga aagaagttaa ggaatacctg
     2461 gaggaactgg ctgcggaagt ggaggaatac cagaaccggc aggaaaagga attgcagcgc
     2521 cgcgaactgg ctcagcttga ggtgaggcag gaagccgtcc aggccggaaa cgcggttgaa
     2581 gagctgaaac ggcgcgaaga gctgatcagc cgcactctgg tcttcgcaga gaaaaccaaa
     2641 gctgacatca tctccaacgc ccgcaaggaa gccgaaaaca tcatccacga ggccgaactc
     2701 agggccaagc gcgccatcca ggaagcgaag cagtatctgg gcatgctgga acagcaatac
     2761 atccagctca aggagcagaa acgccagttc ctgatgcagt tcaggagcga gctcaaaacc
     2821 tttcaggacc gcatagacaa ggacccgctg ctcagcaagc cgccggaaca cattctggat
     2881 tcggatttca agcagatcaa ggaagaaatc gcgcccaacg ccaaagcgcc ggttgaggat
     2941 aaccagttat catgagcgcg gcataccggc aaaccgctga atggctgagg cagcgccttc
     3001 cagaggtccc gaaagtggcc atgatcctgg gcaccgggct gaacggcatc gccgaaaagg
     3061 gaagaatcct ggttcagatt ccctataccg aaatacccgg attcgccagc agcaccgccc
     3121 cttcgcacaa gggaaacctg atcctaggcg aactcggcgg acaaaaagtg ctctatctgc
     3181 aaggccgttt ccatcattac gaaggccatc cgttttccac agtggtcttt cccacgcggg
     3241 tgctggcctg cctgggaatc gaaaccctga tcgtcaccaa cgcctccggc agcctgcggg
     3301 aagaactcag ccccggctcg atcgtaatgc tgaaggacca catcaacttc atgggcggca
     3361 accccattat cggagaaaat gacgaaagcc tgggcgaacg ctttcccagc ctcaaccatc
     3421 cttacgatcc agaatacagg gaagcctgtg ccgccatcgc cgcccgcgcc ggaatcccca
     3481 tgcgcgaagg ggtttacatc gctgtgaccg gtcccagcct ggaaaccagg gcggaatgcg
     3541 cggctttcgc cagttgggga gcggatctgg tgggcatgtc caccgttccg gaagtgatcg
     3601 cggcccggca cgccggaatg aaggtgctgg ccttttccgt cgtgaccaac tacagcaatc
     3661 tgctgcacga ccaggctcat tcccaggaag aaatccgcgc caacgcggac aaagcctccg
     3721 catggctcat cagcatcatc acagaattcg taactggcct gaaatgacgg cgctggatat
     3781 tttcattgac acctggggca gggtcagata tgagggtaaa ctaacgaaaa tgcaaggttc
     3841 acaggagtga cagatggccg ctaaaccaat gtcagaaaag aaactgaaga aatttgaaga
     3901 gctcattcag aaggaactta aagaaagcgt tgcctacatt gaagacgtaa ccaaacacca
     3961 gagcattg
//
LOCUS       DAOOWR010000150         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_22903_length_3966_cov_3.831501, whole genome shotgun sequence.
ACCESSION   DAOOWR010000150 DAOOWR010000000
VERSION     DAOOWR010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22903_length_3966_cov_3.831501"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..2354
                     /locus_tag="PLB85_04550"
     CDS             234..2354
                     /locus_tag="PLB85_04550"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOU72633.1"
                     /translation="MFSKIRKRNGNIVSFDKQKIVQAIRKAGEATGEFEGEAAEVLTA
                     RVVNLLAQIIDSDIPEVERIQDIVEEVLLASPYKKAAKAYIIYRDQHARIREMFSTGG
                     VQLIDKYLEKLDWQVNENSNMAYSLQGLNNYIASEISKTYWLNKIYPPEIRRAHIEGD
                     IHIHDLGQLSVYCVGWDLMDLLVTGFCGAEGKVESSPARHFRSALGQIVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDKLSYEDVKQALQEFIFNINVPTRVGFQTPFTNITLDL
                     QTPDIYKDTPVIIGGEPQTLTYSDFQPEMDMLNKAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFDWDDPKLNYLWEATAKYGIPYFSNFVNSDMDPNDARSMCCRLRLDTRKLEARG
                     GGLFGANPLTGSIGVVTLNLPRIGYRAHSEEEFFTLLEENLQIAKNSLEIKRKILERY
                     TLNGLYPYTRFYLKNIYERFMQYWKNHFSTIGIIGMNEACVNYLGKNLGTSEGRAFAL
                     KVMDYLRERLVDFQEETECNYNLEATPAEGTSYRLAILDKKFYPDIISANCGGETPFY
                     TNSSQLPVNFSDDIFEVLDLQDELQTKYTGGTVLHIFGGERVEHGESIRKLVRTICSS
                     YRLPYFTFSPTFSICPQHGYLNGEQASCPQCQRNTEVFSRIVGYLRPVSQWNEGKKAE
                     FKLRTTFDTIKNSRAKDPAKHHAL"
     gene            2354..3064
                     /locus_tag="PLB85_04555"
     CDS             2354..3064
                     /locus_tag="PLB85_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOU72634.1"
                     /translation="MRIGGFQKFSLLDYPGQLAAIIFTQGCNFRCPYCHNPELVDPGR
                     FEKPLATGRVLDFLRRRRGKLGAVVITGGEPTVQPDLTGFVKKLKFMGFLVKMDTNGS
                     HPDVLQSLVEQKLVDYWAMDIKAPADLYRLVSRSEVPVEDILRSMDILRGSGMEYEFR
                     TTFFERLFDWKDIARVQELLRPGDRFYLQECRYEGTLEDLSSSAADVRKHLQDNPACK
                     TLIRWGDKHQVNIRIRSL"
     gene            3068..>3966
                     /locus_tag="PLB85_04560"
     CDS             3068..>3966
                     /locus_tag="PLB85_04560"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72635.1"
                     /translation="MQEVLIEPWLPLVEKPSRYIDHELNAVRKAWRDVNFCFVYPDVY
                     EVGVSHLGLKILYSIVNRLDGVMADRAYLPWLDMIDIMRRERLPLFGLESRRPLRDFD
                     LVGITLQTELTYSNIPETLSLSDIPVLAKDRAESDPIVMAGGPCATNPLPLSRFIDVF
                     FLGEAEAGIVEIAQIFSRVRDREGRLQELAKLESCYVPALHTGQRVSSRKFTGFGAGG
                     TLHSPQLLSWQLATHNRCVAEIMRGCSRGCRFCHAGYFYRPVRERDAAEVRDEILQEV
                     ALTGWDEAGLLSLSSSDYSRVKEL"
BASE COUNT          903 a         1233 c         1055 g          775 t
ORIGIN      
        1 aaaggcggtg cggcgaccgc ctgatcaggg ccgccaaatc ttttctctgc gggctttgga
       61 ctgctttggg aaaaataaat ccaaaaacgc tcaagtttcg cttgacagaa atgctaactt
      121 cctattatta tacaactcgc agcattggcg cggctatggt taaagtgaga tcaaaactta
      181 taacctgatg cgcgcgagca gattatcgat acgtatcgca agggaggatt gtgatgtttt
      241 cgaagattcg gaaacgcaac ggcaacatag tcagttttga taagcaaaaa atagtgcagg
      301 ccatacgcaa ggcaggcgag gccactggag agtttgaagg cgaagctgct gaagttttaa
      361 cggcgcgggt ggtgaacctc ctggcgcaga tcatagattc cgacatccct gaagtggagc
      421 ggatccagga catcgtggaa gaggttctgc tggcatcccc ctacaaaaag gcggcgaagg
      481 cctacatcat ctaccgcgac cagcacgcgc gcatccgcga aatgttttcc accggcggcg
      541 tgcagctcat cgacaagtat ctggaaaaac tcgactggca ggtgaacgag aattcgaaca
      601 tggcctattc cctgcaggga ttgaacaact acatcgccag cgagattagc aaaacctact
      661 ggctgaacaa gatatacccg ccggaaatcc gccgggcgca tatcgagggc gacatccata
      721 tccacgacct tggccagctt tccgtctatt gcgtgggctg ggacctgatg gacctgctcg
      781 tgacaggctt ctgcggcgcc gagggcaagg tggaaagttc gcccgcgagg catttccgca
      841 gcgccctggg gcagatcgtg aacttctttt acacgctgca gggagaggcg gccggagcgc
      901 aggccttttc caatttcgac acgctgctgg cgccctttat ccgctatgac aagctctctt
      961 acgaagacgt gaagcaggcg ctgcaggaat tcatcttcaa catcaacgtg cccacccgtg
     1021 tcggtttcca gacgcccttt acgaacatca cgctggacct gcagactccc gacatctaca
     1081 aggatacgcc cgtgatcatc ggcggagagc cgcaaaccct tacctacagc gactttcagc
     1141 ccgaaatgga catgctgaac aaggccttcc tcgaagtgat gatcgaaggc gacgccaagg
     1201 gacgcgtttt cacctttccc attcccacct acaacatcac caaagacttt gactgggatg
     1261 accccaagct caactatctc tgggaagcga cggccaaata cggcatcccc tatttctcaa
     1321 acttcgtgaa ttccgacatg gaccccaacg acgcgcgctc gatgtgctgc cgcctgcgcc
     1381 tggacacgcg caagctggaa gcccgcggcg gaggcctgtt cggcgccaat ccgctcacag
     1441 gctccatcgg cgtggtgacg ctcaacctgc cccgcatcgg ctaccgcgcc cacagcgagg
     1501 aagaattctt cactctgctg gaagagaacc tgcaaatcgc caaaaacagc ctcgagatca
     1561 agcgcaaaat ccttgaacgc tataccctga acggacttta cccctatacg cgcttctacc
     1621 tgaagaacat ctacgaacgc ttcatgcagt attggaaaaa ccacttttcc accatcggca
     1681 tcatcggcat gaacgaagcc tgcgtgaact acctgggcaa aaacctcgga acctcggaag
     1741 ggcgcgcctt cgcgctgaaa gtgatggact acctgcgcga gcgtctggtg gatttccagg
     1801 aagagacgga atgcaactac aaccttgagg caacgccagc cgaaggcacc agctaccgcc
     1861 tcgccatcct cgacaaaaag ttctatccgg atatcatcag cgccaactgc ggcggcgaaa
     1921 cgcctttcta taccaacagt tcgcaactgc cggtgaactt ttccgacgac atcttcgagg
     1981 tgcttgacct ccaggacgag ttgcagacca aatacaccgg cggcaccgtg ctgcatatct
     2041 ttggcgggga gagggtcgaa cacggcgaaa gcatccgcaa gcttgtgcgc accatctgtt
     2101 ccagctaccg cttgccctac ttcacttttt cgcccacctt cagcatctgc ccccagcacg
     2161 gctacctcaa cggcgaacag gccagctgcc cgcaatgcca gaggaatacg gaggttttct
     2221 cgcgcatcgt tggctacctg cgccccgtct cgcaatggaa cgagggcaaa aaggcggaat
     2281 tcaagctgcg caccactttc gacaccatca agaacagccg cgcgaaagac cccgcgaagc
     2341 atcacgcgct ctgatgagga ttggcggttt tcaaaagttc tcgctgctcg actatcccgg
     2401 gcagttggcc gccatcatct tcacgcaggg ctgcaatttc cgctgtccct attgccacaa
     2461 ccctgaactg gtcgatcccg ggcgctttga aaagcccctg gcgacggggc gggtgctgga
     2521 tttcctgcgc agaaggaggg gcaagctcgg cgctgtcgtc atcaccggcg gcgaacccac
     2581 ggtgcagccc gatttgaccg gatttgtgaa gaaactgaag tttatgggct tcctcgtgaa
     2641 gatggacacc aacggctccc atccagatgt gctgcaaagc cttgtggaac agaagctcgt
     2701 tgattactgg gcgatggaca tcaaggcccc ggctgacctc taccgcctcg tttcgcgctc
     2761 cgaagtgcct gttgaagaca tcctccgcag catggacatc ctccgcggca gcggaatgga
     2821 atacgaattc cgcaccacct ttttcgagcg cctcttcgac tggaaagaca tcgcccgcgt
     2881 tcaggaactg ctcaggcccg gcgaccgttt ctacctgcag gaatgccgct acgaaggcac
     2941 ccttgaagac ctcagcagca gcgccgccga cgtcaggaaa cacctgcagg acaatcccgc
     3001 ctgcaaaacc ctcatccgct ggggtgacaa acaccaggta aacatccgca tccgttccct
     3061 gtaagctatg caagaggtcc tcatcgagcc ctggctgccc cttgtggaaa agccctcgcg
     3121 ctacatcgac cacgaactca acgcggtgcg caaagcctgg cgggatgtga atttctgctt
     3181 cgtctatccc gacgtttacg aggttggcgt ctcgcatctg gggctgaaga tcctctattc
     3241 catcgtcaac cgcctcgacg gtgtgatggc agaccgcgcc tacctgccct ggctggacat
     3301 gatagacatc atgcgccgtg agcggctgcc cctcttcggc ctcgaaagcc gccgcccgct
     3361 gcgggatttc gacctcgtgg gcatcaccct gcaaaccgaa ctcacctatt caaacatccc
     3421 cgaaacgctg tccctttcag atattcccgt gctggcgaag gaccgcgcgg aaagcgaccc
     3481 catcgtgatg gcaggcggcc cctgcgccac caatcctctg ccgctttccc gcttcatcga
     3541 cgtctttttt ctcggcgagg cggaggccgg catcgttgag atcgcccaaa tcttcagccg
     3601 cgtgcgcgat agggaggggc ggctgcagga actggcaaag ctcgaaagct gttacgttcc
     3661 ggccctgcac acggggcaaa gggtaagttc gcgcaaattc accggcttcg gcgccggcgg
     3721 caccctgcac agcccgcagc tcctttcctg gcagcttgcc acccacaacc gctgcgtggc
     3781 cgaaatcatg cgcggctgct cccgcggctg ccgtttttgc catgccggct atttctaccg
     3841 ccctgtgcgg gaacgcgacg ctgccgaagt gcgcgacgaa atcctgcaag aggtggcgct
     3901 cacgggatgg gacgaagccg gcctgctctc cctttccagc agcgattaca gccgcgtgaa
     3961 ggaact
//
LOCUS       DAOOWR010000151         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23008_length_3952_cov_3.342828, whole genome shotgun sequence.
ACCESSION   DAOOWR010000151 DAOOWR010000000
VERSION     DAOOWR010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23008_length_3952_cov_3.342828"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1490)
                     /locus_tag="PLB85_04565"
     CDS             complement(342..1490)
                     /locus_tag="PLB85_04565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU72636.1"
                     /translation="MSKSYDIVIIGAGSIGVPAALELASKGLKVLVLEAESAPGQGNN
                     KKAIGGVRATHSDFGKMSVCLRSIQILSGWKEQYGEDIGWMSNGYSYPAYTEADEKIL
                     KDLMRVQLGFGLHIRWVSPQEYNELVPGINMDGLRGSTYSAEDGSCSSLLTGSAYYFH
                     ALRAGVDFRFGEKVLGFEMSGDRIAKVRTDKDSYTAGTVINAAGNHAREIGAMAGLDL
                     PVHPDNHEAGITEPVAPFMGPMVVDMRKRPGSANFYFYQNHEGQVVFCITPDPPVLGI
                     DNRSTSKFLPLCSKRMLEVYPRLRHLKVRRTWRGQYPMTPDGFPIVGRVGANLVNAVG
                     MCGQGFMLGPGLAELLARICLDELTPEDLRELESFDPNRDFSGMEAFK"
     gene            complement(1483..3561)
                     /locus_tag="PLB85_04570"
     CDS             complement(1483..3561)
                     /locus_tag="PLB85_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU72637.1"
                     /translation="MENHRLDKHPILSVPEREDVLFTFNGRPLTAKQGEMISSALIAN
                     GISVFGHHHKDGSAQGIFCANGQCAKCTVIANGVAVKSCMTLVTPGMEVRSAEGLPAL
                     PESPVIVQRPAIEQVACEVLIIGGGPSGLAAAIELGKHGIDTLLIDDKHTLGGKLVLQ
                     THKFFGSEEDSHAGVRGHDIGKLLADEVATHPSVKIWLNSTALFIFSDKKVGILKDGI
                     YKIVSPQRILNAAGAREKFLRFTGNNLARIYGAGAFQTLVNRDLIRPTNRLFIIGGGN
                     VGLIAGYHALQAGIEVVGLAEAMPRCGGYKVHADKLKRLGVPIYTSHSILSANGTETV
                     KSVTIAEVDDKFRIKPGTEKSFACDTILIAVGLDSLSEFTQEAMEAGIPVYAAGDALE
                     IAEASSAMFNGKIAGLTIAKDCGHEEIGPVPADWLAKAEVLKSHPGQIKGYQQGLPES
                     GVFPVIHCLQEIPCNPCTTVCPTNSIRTEDGSLLSLPLYDGACIGCNKCLLICPGLAI
                     TLVDFRQDPENPTITVPYEIFNLTKQTGDTIELVDIDGNPLGEYLINAVLDMPNRHTQ
                     LIKVKVPKAIAKRVASFGVQPAEVGQALPQPVIPEKLADDSMICLCERVSVGEVRRLI
                     QSGITNLNQIKAITRAGMGPCGAKTCDTLIRGVLREEGIPVENVVPNTKRPVFVEVPL
                     DKFPDGGSDE"
BASE COUNT          825 a         1032 c         1186 g          909 t
ORIGIN      
        1 gtcaaaccta attctactcc cagcaacgga aagagcggct cggttgagaa agccaagccc
       61 aacgaaaagg agaagcccga aaaagaagaa aagcaggagc aacccctgga tattttccgc
      121 aagctaacag gacgcatcag aaagtaagca agtaacagag ccagataata gcacagagcg
      181 gcccaatccg gccgctcttt ttcatttggc cgttaaggcc ttgtctttag tcacttgcgc
      241 gcctcctgta taatagccgc attcgatgcg agtatctcgc gggaggcggg ggagagggat
      301 ggacgcgacc gctaaggacc agtaaagcct cgcggagagc cctatttgaa ggcttccatg
      361 ccactgaaat cacggttagg atcaaagctc tccagttcgc ggaggtcttc gggagtgagt
      421 tcatccaggc agattcgggc gagcagttct gccagtccgg ggcccagcat gaatccctgg
      481 ccgcacatgc ccacggcgtt gacaaggttg gcgcccaccc tgcccacgat ggggaaaccg
      541 tcaggggtca tgggatactg tccacgccag gtgcggcgca ctttcagatg cctgaggcgg
      601 ggatagactt ccagcatgcg tttgctgcag aggggcagaa acttggaggt gctgcggttg
      661 tcgatgccca ggacgggtgg atcgggggtg atgcagaaaa ccacctgccc ttcgtgattc
      721 tggtaaaagt agaagttggc agagccgggg cgcttgcgca tgtccaccac catcggaccc
      781 atgaaggggg cgacgggttc agtgattccc gcttcatggt tgtctggatg cacgggcaga
      841 tcgagcccgg ccatggcgcc gatttcccgg gcgtggttgc ccgcggcgtt gatcacggtt
      901 ccagccgtgt agctgtcttt gtcggtgcga actttggcaa ttctatcgcc ggacatttcg
      961 aagcccagca ccttttcgcc gaaacggaag tccactccgg ctctgagggc atggaaatag
     1021 taagcggaac ccgtcaacaa ggacgaacag cttccgtcct ctgctgaata ggtggaaccg
     1081 cgcaggccat ccatgttgat gccaggcacc agttcgttgt attcctgcgg gctgacccag
     1141 cgtatgtgca ggccgaatcc aagctggacc cgcatcagat ccttgagtat cttttcgtcc
     1201 gcttcggtgt aggcgggata gctgtagccg ttgctcatcc agccgatgtc ttcgccgtat
     1261 tgttccttcc aaccgctcag gatctggatg gagcgcaggc aaacgctcat cttgccaaaa
     1321 tcgctgtggg tggccctcac gccgccgatg gcttttttat tgttgccctg gccgggggcg
     1381 ctttcagctt ccagaaccag caccttcagg cctttggagg caagctccag agcggctgga
     1441 actccaatag agccagctcc gatgatcacg atgtcatagc ttttactcat cgctgcctcc
     1501 atcaggaaac ttatccagcg gaacttccac gaaaacgggg cgtttggtgt ttgggaccac
     1561 attttccacc gggatgccct cttcgcgcag gacgccccgg atgagggtat cgcaggtttt
     1621 ggcgccacag ggccccatgc ccgcgcgggt gatggctttg atctggttga ggttggtgat
     1681 gccgctttgg atgaggcggc gcacttcgcc cacgctcacc ctttcgcaga ggcaaatcat
     1741 ggaatcgtca gccaacttct ccggaatcac cggctggggc agggcttggc cgacttccgc
     1801 aggttgcacc ccgaaggagg cgacgcgttt ggcgatggcc ttcgggacct ttacctttat
     1861 cagttgggtg tggcggtttg gcatgtccag aacggcgttt ataagatatt cacccaacgg
     1921 attgccgtcg atatccacaa gttctatggt gtcgccggtt tgtttggtca ggttgaatat
     1981 ttcgtaggga acggtgatgg tagggttttc cggatcctgg cggaaatcaa ccagcgtgat
     2041 ggccaggccg gggcagatca gcaggcattt gttgcagccg atgcatgcgc cgtcgtaaag
     2101 tggcagggac agcaggctgc cgtcctcggt gcggatggaa tttgtgggac agaccgttgt
     2161 gcagggattg caggggattt cctgaaggca gtggataacg gggaaaacac cgctttccgg
     2221 caggccctgc tggtagcctt tgatctggcc gggatgggat ttgagcacct cggctttggc
     2281 aagccagtcc gctggaacag gcccgatttc ctcgtggccg cagtctttgg cgatggtgag
     2341 tccggcgatt ttgccgttga acatggcgga ggaagcttcc gcgatttcca gcgcgtctcc
     2401 ggcagcgtaa accggaattc cggcttccat ggcttcctga gtgaattcgg aaagcgaatc
     2461 gaggcccacg gcgatcagga tggtatcgca ggcgaagctc ttttccgtgc cgggtttaat
     2521 ccggaactta tcatccacct cggcgatggt tacgctcttc acggtttcgg tgccattggc
     2581 gctgaggatg ctgtgggaag tgtagatggg cacgccgaga cgcttgagct tgtccgcgtg
     2641 gactttgtag cctccgcatc ggggcatggc ttcagccagg cctaccacct cgattccggc
     2701 ttgcagggca tgatagccgg cgatgaggcc gacgttgccg ccaccgatta tgaagagccg
     2761 gttggtgggg cggatgaggt cgcggttcac cagggtctga aaagcgcccg ctccgtaaat
     2821 ccgggccaga ttgttgccgg tgaagcggag gaacttttcc ctggccccgg cggcgttcag
     2881 aatcctctgg ggtgaaacta tcttgtaaat gccatccttg aggatgccaa cctttttgtc
     2941 gctgaaaatg aagagggcgg tgctgttcag ccagatcttc acggagggat gggtggccac
     3001 ctcgtcggcc agaagcttgc cgatgtcgtg tccgcgcact cctgcgtggc tgtcttcctc
     3061 gctgccgaag aatttatggg tctgtaaaac cagtttgccg cccagggtgt gtttatcgtc
     3121 gataagtaaa gtgtcgatgc cgtgtttgcc tagttcgatg gccgcggcaa gtccggaagg
     3181 gcctccgccg atgatcagaa cctcgcaggc gacctgttcg atggcgggac gctgcacaat
     3241 gacagggctt tccggcaagg ctggcaggcc ttctgcggaa cggacctcca ttccgggggt
     3301 gaccagggtc atgcaggatt tcacggccac gccgtttgcg ataactgtgc atttggcaca
     3361 ctggccgttg gcgcagaaaa tgccctgggc gctgccgtcc ttgtggtgat ggccaaaaac
     3421 gctgatcccg ttcgcaatga gagcggagga gatcatctca ccctgcttgg cggtgagggg
     3481 cctgccgttg aaagtgaaaa gaacatcctc ccgctccggg acggagagga tcggatgctt
     3541 gtcgagccga tggttttcca tcaatggaaa agctccttgg aaagattaat tgtgtttcaa
     3601 aaagtttcag aggccgttcg tgtcaagcaa aagctggttc tggcatttat gggtatgttc
     3661 attatgcggc ttaacgacat gatattccag acgaaggaaa aacaatgcca tatgtttcaa
     3721 ctggttattc tgaattcata atcccggact gggtgtgatg acacaacttt ctttctcgcc
     3781 ttgaaatcgg gaagatggga aaaccgactt tataaattta tcagtctatc agtttctttc
     3841 cacggcccct tcccgacctt ccagtccagg acctggataa tatgtatcta atataccgat
     3901 atatatacta gatagatata atatatacta atatagtagg tattagtagt ta
//
LOCUS       DAOOWR010000152         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23018_length_3950_cov_3.797689, whole genome shotgun sequence.
ACCESSION   DAOOWR010000152 DAOOWR010000000
VERSION     DAOOWR010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23018_length_3950_cov_3.797689"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="PLB85_04575"
     CDS             complement(<1..2331)
                     /locus_tag="PLB85_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72638.1"
                     /translation="MKRTLLLIGLLALCGLAFGLSLHLNVDPAGATGATRGAETAPLL
                     DPGVPALNYLPVRVLLPFGEKVLNVSVNLDEPLLHDDRAELDFVRAQQKISQPHPDTT
                     QPDPAIWRVDAAWPSQDFSYLGVQSMRGYQIAVINVYPFKYNPVKKHLLASTQVQIEI
                     ETTFDEATARASANFFAPARGNSDLQDLVLNPDAATSYLSSASYRDHVPQSRLIDLSV
                     PKKMIIITSDSCTSNFTNYIQWRNTQNISTGLFSTADIYDAYLGADNAEKVRNFIIDA
                     YQTWATSATPLEYVILGGDDEIVPERGCYGQVGDTVDNRMPTDIYFSNLDGNWNANGN
                     NIWGEPSDDVDMLPEVHIGRFPAETQSEFANIFRKTQYYVNNNTFSNNISIMFGENLN
                     YNPLTWGGDYKDDVAIHIPDDYFLRTMYQRDGTYSAEGVWNAINQGANVMNHMGHANE
                     TYLMGQSNNTIQQLQNTEYGFLYSQGCYPAAFDQRTSGISECIGEHLSFANGGVFAFI
                     GNTRYGWYMPGSIDGPSQFYDREFFIGLYETPNTRLGQALSYSRLQNLNAALSIDVMR
                     WCYYQMVLFGDPSIEIKYPDTSLPFLTLEDYSISDIEGDGDGGINPGEILRIFPVIRN
                     HQDWATANNVSVTLQNVPAGVEVLTGTQSISQILPGETSPDGFFIRVQLSPDIGYGIY
                     QMQLALDAVHPTTGLSIGIRRFDISVEVTMVDNRYPWDCQIQTKSAPMVHDFNRDGSS
                     DIMYLDVYGRAYYIDGYGENFDGWFAPPQQDIMSSAA"
     gene            3069..>3950
                     /locus_tag="PLB85_04580"
     CDS             3069..>3950
                     /locus_tag="PLB85_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72639.1"
                     /translation="MVGIVKTYTTIGIDGQLLTVECDSAGQMYSVNIVGMASNAVKES
                     KDRIFAALSNSGFEAAIKRFTINLAPADIRKDSAALDLPIAISVLQSIKQLPVEYLSQ
                     TAIIGELSLDGNLRPVKGVLPIALAAKRDGMDALVLPYENAEEAAIIEELKVIPVTSL
                     REAVLYLRGEIEIKPATVDRDKIFSVLNDFPVDMHDVKGQYQVKRALEVSAAGGHNLL
                     MIGPPGSGKTMLARRVPTILPELSLDESLEATKIHSVAGYSKHFKNGILTTRPFRAPH
                     HSCSENGLIGGGTFPRPG"
BASE COUNT          932 a         1027 c         1092 g          899 t
ORIGIN      
        1 ggcggcgcta ctcatgatat cctgttgcgg aggagcgaac cagccatcga aattctcgcc
       61 atacccatca atgtaatagg cccggccata gacatccaga tacatgatat cagagcttcc
      121 gtcgcggttg aaatcgtgca ccatgggcgc ggatttggtt tggatctggc aatcccaagg
      181 atatcggtta tccaccatgg tgacctctac actgatgtca aacctgcgaa taccgatgct
      241 gagaccggtg gtaggatgca ccgcatccag cgcaagttgc atctggtaga ttccatagcc
      301 gatgtcgggg cttaattgca cgcggatgaa aaatccatcc gggctggtct cgccaggcag
      361 gatctgggag atgctctgtg taccggtcag cacctctact ccagccggga cattttgcag
      421 ggtcacgcta acattattgg ctgtcgccca gtcctgatgg tttcggatca caggaaagat
      481 gcgcagaatt tctccggggt taatgccgcc gtcgccatca ccttcgatat cggagatgct
      541 gtaatcttcc aaagtaagga aaggcaggga ggtatcggga tacttgatct ctatggaagg
      601 atcgccgaac agcaccatct ggtagtagca ccagcgcatt acgtctatgc tgagcgcggc
      661 attgaggttt tgcagccggg aatagctgag cgcctggccc aggcgggtgt tcggggtctc
      721 gtacaggcct atgaaaaact cgcgatcata aaactgcgag gggccatcaa tgctgcctgg
      781 catgtaccag ccatagcgcg tgttaccaat gaaggcgaaa accccgccat tcgcgaagga
      841 caggtgttcg ccgatgcatt cgctaatccc actggtccgc tggtcaaaag cggcgggata
      901 gcatccctgg ctgtaaagaa atccgtattc cgtgttctgg agttgctgga tggtgttgtt
      961 gctttgcccc atcaggtagg tttcgttggc gtggcccatg tggttcatga cgttggcgcc
     1021 ctggttgatg gcgttccaga cgccctccgc gctgtaggtg ccgtcacgct gatacatggt
     1081 gcgcaggaaa taatcatctg gaatatggat ggccacatca tccttgtaat cgccgcccca
     1141 agtgaggggg ttgtagttaa gattttcgcc gaacatgatc gagatgttat tgctaaatgt
     1201 gttgttgtta acgtagtatt gggttttacg gaagatgttg gcaaattcag actgagtttc
     1261 ggctgggaaa cgtccgatgt gcacttcagg cagcatgtcc acgtcgtccg atggttcgcc
     1321 ccagatattg tttccgttgg cattccagtt cccatccaga ttgctgaaat agatgtcggt
     1381 gggcatccgg ttgtccacag tgtcgcccac ctgaccgtag cagccgcgtt ccggcacgat
     1441 ctcgtcatcg ccgcccagga tcacgtattc cagcggagtg gctgaggtag cccaagtttg
     1501 ataggcatcg atgatgaagt tgcgcacctt ctcggcgttg tcagctccga ggtaggcgtc
     1561 atagatatcc gctgtgctga acaggccggt acttatgttc tgtgtgttgc gccactggat
     1621 atagttggtg aaattcgagg tgcaggaatc gctggtgatg atgatcatct ttttgggcac
     1681 gctgaggtct atcaaccggc tttgcggcac gtggtcgcga tagctggctg aactcaggta
     1741 actggtagcg gcatccggat tgagcaccaa atcctgtaag tcagaattgc ccctggctgg
     1801 ggcaaaaaag ttggctgagg cacgcgccgt ggcttcgtca aaggtggtct cgatctcgat
     1861 ctggacctgt gtactggcca gaagatgctt cttcacggga ttgtatttga agggatagac
     1921 gttgatcacg gcaatctgat aaccgcgcat ggactgcact cccagatagc tgaaatcctg
     1981 gctgggccag gcagcatcga ctctccagat agcggggtcc ggttgcgtgg tgtcaggatg
     2041 gggctgcgaa atcttttgct gcgcgcgaac gaagtcgagt tcagccctgt cgtcatggag
     2101 cagtggctcg tccaggttca cactcacgtt cagcactttt tcgccgaagg gcagcagcac
     2161 ccgcaccgga agatagttca gcgccggaac gcctggatcc agcaggggcg cggtctcagc
     2221 accccgggtg gcgcctgtgg cgccggcagg atcaacattc aggtgcagac tcagcccgaa
     2281 agcgagcccg caaagggcca aaagcccgat cagtaaaagc gtccttttca tatctatcat
     2341 ccttggctca gataaattac acgtttttgg caatgcaacc cataaactac aaacggccgg
     2401 gaaacgtcaa gcattttttc tcttcgcact tgcacacaca aatcagaaac tcaacgtaag
     2461 caaaacccat acagatattc ttctttcccc tgcataaaac aaaccagctc ctgctcagta
     2521 tagccattca ttaacttaga taatgcatta gatatctcag acacaaattc aggactgaaa
     2581 ttatcagctc atattcaaag agtggcgtaa tcgcagtaat cccagcttag tggtcctcgc
     2641 aaacgagcat agcgaattcg tgggatacga agcggaaaga aaacccgcta ggaagtgaag
     2701 cgatgttgac tggccgttaa gaccctttac caaatccctg gtgcgcctcc cgtatgatac
     2761 ccgcatttga tacgagaata atgcgggagg catgagagtg ggatggactc aaccgctcag
     2821 gacatggcag acaggacatg gcagtagata agcatttctt tatccctcaa gactttacta
     2881 aaacggtttc aacgagagcc aaatagaaac ttttcaagca tatgggccag gctattgctg
     2941 tctgatgact acttattttt ccgagaaaag gtgctactga cttggaggaa acagattttt
     3001 ccttgaccgc taaggcgaat gttgattagt gtgaccggag ctgacaaaca gagcaaggac
     3061 tcaagtaaat ggtaggtatt gtaaagacat acaccacgat cggaatcgac ggccagctgc
     3121 tgacggtgga atgtgacagc gccgggcaga tgtattccgt gaacatcgtg ggcatggcat
     3181 ccaacgccgt gaaggagagc aaagaccgga ttttcgcggc actcagcaac tccggtttcg
     3241 aggccgcaat aaagcgtttc accatcaatc tggccccggc cgacatcagg aaagacagtg
     3301 ccgcgctgga ccttcccatc gccatttccg tgctgcagag catcaaacaa ctgccagtgg
     3361 aatacctcag tcaaaccgcg attatcggcg agctctcgct ggacggcaat ctgcgccctg
     3421 tgaagggcgt tttgcccatc gccctggccg ccaagcgaga tggcatggat gctttggtgc
     3481 tgccttacga aaatgccgag gaagccgcca tcatcgagga attgaaagtg atcccggtga
     3541 cctcgctgcg agaagcggtg ctctatctgc gtggcgaaat cgagatcaaa ccggcgacgg
     3601 ttgacaggga caagatattc tccgtgctta acgattttcc cgtggatatg cacgacgtga
     3661 aaggccagta tcaggtgaag cgcgccttgg aggtctcagc cgcaggaggc cacaacctgc
     3721 tgatgatcgg ccctcccggc agcggcaaga ccatgctggc gcgccgggtg cctacgatat
     3781 tgccggagct ctctctggac gagtctttgg aggccaccaa gatccattcc gtggccggct
     3841 attccaagca cttcaaaaac ggcatcctca ccacccggcc tttccgcgct ccgcaccact
     3901 cctgcagcga aaacgggttg atcggtggcg gaactttccc ccggcccggg
//
LOCUS       DAOOWR010000153         3898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23421_length_3898_cov_3.750195, whole genome shotgun sequence.
ACCESSION   DAOOWR010000153 DAOOWR010000000
VERSION     DAOOWR010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23421_length_3898_cov_3.750195"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLB85_04585"
     CDS             <1..542
                     /locus_tag="PLB85_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72640.1"
                     /translation="GRIDEDYILPSEISLGLNYQPRNMLMRTRFNLETQWVKFSDIST
                     HYEDCLNFSAGVEHHITNRLPLRLGFSSENSYLRFVEPDGAIIARKIITPTVTGGSSI
                     ILADNLSLDLGIGYSWREYEALDLFKDSFYNDKHYTGNNTYLLWPNQYIVLSDRGWEN
                     PDKVRESNVSLSASLSVSW"
     gene            547..2205
                     /locus_tag="PLB85_04590"
     CDS             547..2205
                     /locus_tag="PLB85_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU72641.1"
                     /translation="MKKIILLTALSLILTVGLLAEDLRLDIVFSNDIHGGIDRSEATF
                     MNPDFPPQLGGGASAATLIKHIRSLSGTKRDMLLFDAGDFFQGRPVGTVTKGRAVIEY
                     MNAIGYDAMTIGNHEFDIMNDELMETLDYANFPILSCNIIDRRTGDMPWYVVPYRIIS
                     RLGLRIGVIGFTTTDTEKMSFPEHIKNIEFLDEKDTVAKYVKLVREQERADIVIVLGH
                     AGLPYEVEATYLSRYDAAGNPLQQERYAAWGWDAQEIAREVPGIDLFVGGHIHKGWPE
                     PWIDPHTHTMVVQGYAYGSNLGWITLTVDPATRSVSGYELPALREGMMITLFEDQFIP
                     DREISATIEAEVAKAEEGMDEIIGIAGVHLNRTNVDAQSLMGNTIVDAMRNEVNADFA
                     FLNLGGVRADIKNGPVTYRDIFQVMPFDNMVVSFRCTGEFLRRIIETRVEGGRHGLVV
                     SGVNVVYSKKRPNFDRVTSLKIGGQPWDRNKIYTCTTTDFLMQGNAGLTLLTQVPQED
                     IVFHQINLRDAIVNYFRQKSPIRTKIDDRWKRDDNAKPTKEMLQ"
     gene            2202..2687
                     /locus_tag="PLB85_04595"
     CDS             2202..2687
                     /locus_tag="PLB85_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOU72642.1"
                     /translation="MNVQLVICSHSGNTQKLGELVKAKLTEAGHRVDLTRLACDPPLD
                     QSKPTEAKNIRITNLPDVASADIVLIGGPVWAFRPYPLLLKAMADLGPQLRGKRVMPF
                     VTHSFPWAWLTGNSSLKTLRELASRAGAQVLPGVVLSGASSPKTAKYEEAANRICAQV
                     K"
     gene            complement(3101..3460)
                     /locus_tag="PLB85_04600"
     CDS             complement(3101..3460)
                     /locus_tag="PLB85_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72643.1"
                     /translation="MIRIFTLEYDPIKKSFDDSALSTFLAGKSVISIEKRFFKQNSRF
                     FWTFAIEYETEKSGNTKDIEFDADDQKGLFLALKNGVMNWLPKEESRPICFLPITNSS
                     KLPLNSQRPEKNLKTSR"
BASE COUNT          914 a         1150 c          991 g          843 t
ORIGIN      
        1 ttggcagaat cgacgaagac tatatcctgc cctccgagat cagtctgggc ctgaactatc
       61 agccgcgcaa catgctcatg cgcacccggt tcaacctgga aactcagtgg gtgaagttct
      121 ccgatatctc cacccattat gaggactgcc tcaatttctc ggccggggtt gagcatcaca
      181 tcacgaaccg cctgcccctg cgcctgggct tcagctcgga aaacagctat ctccgctttg
      241 tggagccgga cggggccatc atcgccagaa agataatcac ccccaccgtc accggcggca
      301 gcagcattat ccttgcggac aacctgagcc tcgacctcgg cataggctat tcctggcgcg
      361 aatatgaggc gctggacctc ttcaaggatt ccttttacaa tgacaaacac tacaccggca
      421 acaacaccta tctgctctgg ccgaaccagt acatcgtact ctccgaccgc ggctgggaaa
      481 acccggacaa ggtgcgtgaa agcaatgtca gcctgagcgc cagcctcagc gtatcctggt
      541 aagcttatga aaaagataat cctgctaacg gcgctgagcc tcatcctcac ggttgggctg
      601 ctcgccgaag acctccggct ggatatcgtt ttttccaacg acatccatgg cggcattgac
      661 cgttcagagg caaccttcat gaacccggat ttcccgcccc aactgggcgg aggggcttcc
      721 gcagccaccc tcatcaagca tatccgctcc ctctccggga ccaagcgtga catgctgctg
      781 ttcgacgccg gcgacttctt ccaggggcgc ccggtgggca ccgtgaccaa aggccgcgcc
      841 gtgatcgaat acatgaacgc catcggctac gacgccatga ctatcggcaa ccacgaattc
      901 gacatcatga acgatgaact gatggaaacc ctggactacg ctaatttccc catcctttcc
      961 tgcaacatca tcgaccgccg caccggcgac atgccctggt atgtggtgcc ttaccgcatc
     1021 atcagccgac tcggcctgcg gataggagtg atcggcttca ccaccacgga tacggaaaag
     1081 atgagcttcc ccgagcatat caaaaacatc gagttcctgg atgaaaagga cacggtggcc
     1141 aaatatgtga aactcgtgcg ggagcaggaa cgggcagata tcgttatcgt tctgggccat
     1201 gccggcctgc cctatgaggt tgaggcgacc tacctttccc gttatgacgc ggcgggaaat
     1261 cccctgcagc aggaacgcta cgccgcctgg ggttgggacg cccaggaaat cgcccgcgaa
     1321 gttcccggca tcgatctctt cgtcggcggc cacatccata aaggctggcc cgaaccctgg
     1381 atcgatcccc acacccacac catggtggtg cagggctatg cctacggctc aaacctcggc
     1441 tggataacgc ttaccgtcga tccggccacc aggagcgtga gcggctacga actgcccgcc
     1501 ctgcgcgaag ggatgatgat aaccctgttt gaggaccagt tcatcccgga ccgggaaatc
     1561 tccgccacca tagaggccga ggtcgccaaa gccgaggaag gcatggacga gatcatcggc
     1621 atcgccggtg tccacctcaa ccgcacaaac gtggacgccc aaagcctgat gggaaacacc
     1681 atcgtggacg ccatgcgcaa tgaagtcaac gcggatttcg cctttctgaa cctcggcgga
     1741 gtacgcgccg acattaaaaa cggacctgtc acttaccggg acatctttca ggtgatgccc
     1801 ttcgacaaca tggttgtcag tttccgctgc accggcgaat tcctgcgccg catcatcgaa
     1861 acccgcgtgg agggagggcg ccacggtttg gtggtctccg gcgtcaatgt ggtgtattcc
     1921 aaaaaacgcc ccaatttcga ccgcgtcact tcgctcaaga tcggcggcca gccctgggac
     1981 aggaacaaga tctacacctg caccaccacg gacttcctga tgcagggaaa tgccggcctg
     2041 acgctgctga cccaagtgcc ccaggaagac atcgtcttcc accagatcaa cctgcgtgac
     2101 gccatcgtga actacttccg acaaaaaagc cccatccgca ccaaaatcga cgaccgctgg
     2161 aaacgcgatg acaacgccaa acccaccaag gagatgctgc aatgaatgtt caactcgtga
     2221 tttgttccca ttccggcaac acccaaaaac tgggggagct ggtgaaagcc aaactgacgg
     2281 aagccggaca ccgggtcgat ctgaccaggc tggcttgcga tccgcccctg gaccagagca
     2341 agcccacgga agccaaaaac atccggatca ccaacctgcc tgatgttgct tcggcggata
     2401 tcgttctgat cggcggcccc gtctgggctt tccggcccta tccgctgctg ctgaaggcga
     2461 tggcggacct gggcccccaa ctcaggggaa agcgggtcat gcctttcgtg acccactctt
     2521 ttccctgggc ctggctgacg ggcaactcct ccctgaaaac cctgcgcgaa ctggcttccc
     2581 gggccggagc ccaggtgctg ccgggagtgg tgctgtccgg cgcctccagc cctaaaaccg
     2641 ctaaatacga agaggccgcg aacaggatct gcgctcaagt caaataaccg ttggcgtcgg
     2701 gcgttgatat gcttgtatgg gcggttgccg aaccgccttt tcctctttcc agtccgtctg
     2761 atccacagat aaaaaagttg gtgtggagga gtggtcggaa aaacgggacc gtgttatgta
     2821 cacttgggca tacttcccca gccggttatt actttcatac aagatatata ttgacaagcc
     2881 ataaagcttt attcttttga tgatggctgt aatggtagat gtcttcagag acttgcataa
     2941 caataccgtt gctttattat gccgctgtat tcaaaatcca gtccagggta tcaagccatt
     3001 ggacatagat cggatactgc tgattcttgc tcatcactaa tgttctcact gattatcttc
     3061 aacacggtct caccatattc ctgcaccttc tttgcgctta tcatctggat gtttttaagt
     3121 tcttctctgg tctttggctg tttaatggca atttgcttga gttggttatt ggtaaaaagc
     3181 agatagggcg ggactcctct ttcggcagcc agttcattac gccattcttg agggcaagaa
     3241 acaagccctt ctgatcatcg gcatcaaatt caatgtcttt ggtatttcca gatttctcag
     3301 tttcgtactc gatggcaaag gtccagaaaa aacgcgagtt ctgcttgaaa aagcgctttt
     3361 cgatgctgat tacactcttg cccgccagaa aagtgcttag cgcgctgtcg tcaaaactct
     3421 tcttgatcgg gtcgtattca agcgtgaaga ttctgatcat gatgcatggc tccttgcgct
     3481 gatcttcttt tctttacaaa agaaaagaag caaaagaaaa cctgatctac gcgtttaacg
     3541 catagccacc ttcgctccct tgctgcgctg aggggagctt aaggtggcta tgcttaaccg
     3601 ggttctaaac tttaacattt tcttaaggta aaaaggtaag aaacctaaaa tcaagggact
     3661 gccctgcaga ggcggaagcc gatagcgtcg ctgctgtacg gggtgctgct gaaccgatac
     3721 gcaacccggc agtagtaggc actaccgccc cagccaccgc cccgtagcac acggtacgac
     3781 ccgctcgcag gaccagtcgg attgttctgc aggctgctat tgtaataaga gctggaatac
     3841 cagtcccagc accattcata gacattaccg ctcatgtcgt aagtgccgat gccgttgg
//
LOCUS       DAOOWR010000154         3870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23618_length_3870_cov_3.207339, whole genome shotgun sequence.
ACCESSION   DAOOWR010000154 DAOOWR010000000
VERSION     DAOOWR010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23618_length_3870_cov_3.207339"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="PLB85_04605"
     CDS             <1..1694
                     /locus_tag="PLB85_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU72644.1"
                     /translation="TSFASADYIGTAIPNTPGDLVCLVGWYNAAQVTPRNLADFLNFT
                     TAPYMEVDPAILDGFSYVVGSGPSTAQSFTVSGADLTADIILTAPTHYEISLSETTGY
                     TSPLTLTQTGGAVPTTTIYARLKSGLAVGEYNNEVINITSTGATALTVTCSGEITAPP
                     PPDAPVALEETNVGLTSFKANWEASTGATGYYLDVFTITPAIATDLIISEYIEGSNNN
                     KALEIYNGTGVDVDLSDYTIHLYSNGSSTPSTTNILEMTGTLANGDVYVVAHASAAPE
                     VQANADTTSTVTYYNGDDAIALYKESTASNVDIFGCIGEDPGAAWTSGDHSTVDKTLV
                     RKLTVTGGVSVNPSAGFPTLTTEWDVYPVNTFSYLGYHVMGTFNYVAGYQNLNVGNVT
                     SYTVSGLTAGTTYYYVVRAYNAQGSSDNSNTIEVNSVGGMITISPIQENELFNLPGYP
                     SDEYNTYQVVGSNLIDPITVAAPTHFEVSTSANIGFAQTIELPYNFSGNIYVRLNSLV
                     VGTHDGLLTLVSDGVQASQQLKARLLSLLSLGISSITLPPSPTRWELPPPIKPTL"
     gene            1658..2677
                     /locus_tag="PLB85_04610"
     CDS             1658..2677
                     /locus_tag="PLB85_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU72645.1"
                     /translation="MGTPSAHQTYTLSATGTTVGATLNLTMSGPFEISTSASGPWSAT
                     LELAYNFNGSVYVRMNSNVDGYFTGSILHETANASPATVELEGTAIPAVGYLASELFF
                     SEYIEGSGNNKAIEIFNGTGAPVDLSDYQFENWYNGLLTPSTVPLSGTLANGSVIVLA
                     NPAASAEILALAAITSGNVSFNGDDALALRKISTDTLVDIFGRIGEDPGTEWTADGGY
                     STLNKTLVRKPTVTGGVTENPATGFPTLSTEWNVYDVDTFTYLGSHDFNPGSGLDSPV
                     VQISQAGGIITLTWDAITGATAYEIWSSTDPYGTYTLAQGNWTSTTWTESSAAMKFYK
                     VIAKN"
     gene            complement(3051..3467)
                     /gene="mce"
                     /locus_tag="PLB85_04615"
     CDS             complement(3051..3467)
                     /gene="mce"
                     /locus_tag="PLB85_04615"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU72646.1"
                     /translation="MIKHISHIGIAVKDLEEGIAFYEKLGLTLEGTEEVASQKVKVAF
                     FPCGDTRIELLAPTSEDSPIAKFLEKKGEGIQHIAFAVDDLPKELQKAEENGIRLIDK
                     EPRPGAHHADIAFLHPKSTLGVLIEFCQEAEHKEEK"
     gene            complement(3472..>3870)
                     /locus_tag="PLB85_04620"
     CDS             complement(3472..>3870)
                     /locus_tag="PLB85_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU72647.1"
                     /translation="SRADVIISDEEIYFPEATNFDCLLALTQEACDKYLFDLRERGIL
                     IIDTTYVKNLALAAENTYELNFTEIAIEKLGSPITTNILSLAFLVKVTGIVKEASLKQ
                     SISETVKPAFVDLNLKAMELGFELAKNYGK"
BASE COUNT          855 a         1114 c         1000 g          901 t
ORIGIN      
        1 ggacatcctt tgcaagtgcc gactatatag gtaccgcgat cccaaacacc ccgggcgact
       61 tagtctgcct ggtaggctgg tataacgctg cgcaagttac tccccgcaat ctagcggact
      121 tcctcaattt caccaccgct ccttatatgg aagtcgatcc agccatacta gacggcttca
      181 gctatgttgt cggttcgggc ccttccaccg cccagagctt caccgtcagc ggagcggacc
      241 tgactgccga catcatccta acagcgccta cccactatga aatctcactg agtgagacca
      301 caggttacac ttcacccctc accctcaccc agactggcgg agcggttccc accaccacca
      361 tctacgcgcg cctgaaatca ggtctggcag ttggcgaata caacaacgag gtgataaaca
      421 tcacctccac tggtgccact gccctgacag tcacctgtag cggcgaaatc actgccccgc
      481 cgcctccgga tgcccctgtg gccttggaag agacaaatgt tggcctcacc agcttcaaag
      541 ctaattggga agcatcaacc ggtgccactg gttattatct ggatgttttc acaataaccc
      601 ccgcgatagc tactgacctc atcatatctg aatacattga aggcagcaac aacaacaaag
      661 ctctcgagat ttataatggc actggtgtgg atgttgacct gagcgattac accatacacc
      721 tctatagcaa tggttccagc actccctcca ccaccaacat actcgaaatg acaggtacgc
      781 tggccaacgg tgatgtgtat gttgttgcgc atgcttctgc tgcgccagag gtgcaggcca
      841 atgcggacac cacatcaacc gtaacctact acaacggtga tgacgctatt gctctgtaca
      901 aagaatccac agcctccaat gtcgatatct ttggctgcat cggtgaagat cccggcgcag
      961 cctggacttc aggtgaccat tccacggtcg acaagacttt ggtgagaaaa ctcacggtta
     1021 ccggtggcgt ttctgtgaat ccctcggcag gatttcctac tttgacaact gaatgggatg
     1081 tttacccggt caacacgttc tcatatctgg gctatcatgt catgggcacc tttaattacg
     1141 tggctggcta ccagaacctc aacgtaggca acgtgacttc ttacaccgtt tccggcctca
     1201 ccgccggcac cacctactat tatgtggtgc gggcttataa tgcccaaggc tccagcgaca
     1261 actccaatac gatcgaagtc aactccgtgg gcggaatgat cactatctca ccgatccagg
     1321 aaaacgagct cttcaacctg cccggatacc cttccgacga atacaacacc tatcaggtgg
     1381 tgggcagtaa cctgatcgat ccgatcacag tggctgcacc cacccatttc gaggtatcaa
     1441 catccgcgaa cattggcttt gcccaaacca ttgagctgcc ttacaacttc agcggcaata
     1501 tctacgtcag gctgaacagc ttggtagtcg gcacgcatga cgggctcctg acccttgtca
     1561 gtgacggcgt tcaggcatcc caacagctga aggcgagact attgagccta ctgtcacttg
     1621 gaatatcatc gataacctta ccgccttctc ccacgaggtg ggaactccct ccgcccatca
     1681 aacctacact ctgagcgcga cgggcactac tgtaggcgcc acgcttaacc ttactatgag
     1741 cggccccttc gagatcagca cttcagcttc cggcccttgg tctgccacgc tggaactggc
     1801 ctacaacttc aatggcagtg tgtatgtgcg catgaattcc aacgtggacg gctatttcac
     1861 cggttccatc ctgcacgaga cggccaatgc cagtcccgcc acagtagagc tggaaggtac
     1921 cgccatacca gctgttggat accttgccag cgaactcttc ttctccgaat acatcgaagg
     1981 ctcaggcaac aacaaggcca tcgagatatt caatggcacc ggcgctccgg tggacctgag
     2041 cgactatcag tttgaaaact ggtacaatgg tctgctaacc ccgagcactg tgccactttc
     2101 aggaaccctt gccaatggga gtgttattgt cctggctaac ccagcagcct ctgcagaaat
     2161 cttggctttg gcagctatca cttccggcaa tgtttccttc aatggagacg acgcgctggc
     2221 tctgcgcaaa atcagcacgg acacattagt ggacatcttc ggccgcatcg gcgaagaccc
     2281 tggcacagaa tggactgctg acggcggcta ttccaccctg aacaagacac ttgtccgcaa
     2341 acctaccgtc accggtggcg ttacagagaa tccagcgaca ggcttcccca ccctctcaac
     2401 tgaatggaat gtatacgatg tcgacacctt tacctatctg ggcagccacg actttaatcc
     2461 cggcagcggt ctcgattctc ccgtggtaca gatcagccag gctggcggca tcatcaccct
     2521 cacctgggat gctatcactg gcgcaaccgc ctacgagatt tggtcctcca ccgaccccta
     2581 cggcacttac accctcgccc agggtaactg gaccagcacc acttggacgg aaagctctgc
     2641 ggcgatgaag ttctacaagg tgatagccaa gaactaaccc aataaccaac aaccacataa
     2701 gcatacccgg gagtctgctc ccgggttttt tgtcagctcg tcctttgccc tgtccctttc
     2761 accccctgtc tgcctcccgt ttagttcccg tttgaaaggc gggaactaaa cgggaagtga
     2821 atgggatgtg aatgggaagg ggcaaatgga gtagggacgg tttgaggttt tggtgtgggg
     2881 cgatgcccaa caatctttca ccctatctcg ctgtggttga tgctgttgcg tcgctctacc
     2941 ccagcctttc tgcccagaaa ggcgggtttg gtgttcggca cagaaaaagg ccggcgttga
     3001 ggccggcctg gatattgtgt cccggaggca gaaaccgccg ggctatgggg ttacttttct
     3061 tctttgtgct ccgcttcctg gcagaattcg atcagcacgc cgagggtgga tttgggatgc
     3121 aggaaggcga tgtcggcatg atgggccccg gggcggggtt ccttgtcgat gaggcggatg
     3181 ccgttctctt ccgctttctg tagctctttg ggcaggtcgt ccacggcgaa ggcgatgtgt
     3241 tggattccct cgcctttttt ctccaggaat ttggcgatgg ggctgtcttc ggaggtgggg
     3301 gcaagcagtt cgatgcgggt gtcgccgcag ggaaagaaag ccactttcac cttttgggaa
     3361 gccacttcct cggtgccttc gagggtgagg cccagctttt cgtagaaggc gatgccttct
     3421 tccaggtcct tcaccgcgat gccgatatgg gaaatgtgtt tgatcatggc atcatttccc
     3481 gtagtttttg gccagttcga agccgagttc cattgccttg aggttcaggt ccacaaaggc
     3541 tggtttcacg gtttcggaga tggattgctt cagcgaggcc tccttcacga ttccggtgac
     3601 cttcaccagg aaagcgaggg agaggatgtt cgtggtgatg gggctgccga gtttctcgat
     3661 ggcgatttcg gtgaagttca gctcataagt gttctccgcg gcgagagcga ggttttttac
     3721 gtaggtagtg tcgatgatga ggatgcctct ttcgcggaga tcgaagagat acttgtcgca
     3781 agcttcctgg gtgagggcca ggaggcagtc gaagttcgtg gcttcgggga aatatatctc
     3841 ctcgtcgctg atgatcacgt cggcgcggga
//
LOCUS       DAOOWR010000155         3869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23622_length_3869_cov_4.383325, whole genome shotgun sequence.
ACCESSION   DAOOWR010000155 DAOOWR010000000
VERSION     DAOOWR010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23622_length_3869_cov_4.383325"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLB85_04625"
     CDS             complement(<1..197)
                     /locus_tag="PLB85_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOU72648.1"
                     /translation="MLVPDELYYTESHEWVSVKDGIATIGITDFAQHELGDIVFLELP
                     DAGAKVSAGEPCGTIEAVKSA"
     gene            407..1264
                     /locus_tag="PLB85_04630"
     CDS             407..1264
                     /locus_tag="PLB85_04630"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOU72649.1"
                     /translation="MTTNTASKRILAVHDLSSFGHTSLMAFIPIMYRLGIRVCALPTA
                     ILSANTDFPNPLWIDFSGQIEPFADHWEALELRFDAVSTGFLASAEQAELVGKAIDRL
                     KQLGTLVAVDPVMGDQGSLYSCFGPPLVDAMRRLVAKAELITPNFTEAALLAGADPTP
                     RAEPDEVRRWCQAIAASGPEQIVVTSVPTADPSQLEVLHYNAPEDSLSRLPFAVSPGH
                     KPGAGDCFAALLLAGLVNGHSLPTSLKATVRIMSRAILEELPPGADWREGIAMENMIQ
                     WDLQAYYRE"
     gene            1267..1596
                     /locus_tag="PLB85_04635"
     CDS             1267..1596
                     /locus_tag="PLB85_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOU72650.1"
                     /translation="MKTIILLTISNIFMTFAWYGHLKYKNTPLFTVILISWGIAFFEY
                     CFQVPANRIGHGVFNAYQLKTIQEVITLLVFSVFSVLYLKEEFRWNYLVGFLFLIGAV
                     FFIFKKW"
     gene            complement(1604..2692)
                     /locus_tag="PLB85_04640"
     CDS             complement(1604..2692)
                     /locus_tag="PLB85_04640"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU72651.1"
                     /translation="MIHLPTRIFFGEGALLKAADQLLSLGSRPLVVTGRSSARLSGAL
                     DELSLVLDRAGLAWELFDQVEENPSLDTVLAGARLLRESGCDFLISIGGGSPIDAAKA
                     ISLAAANSLGRDEIYQTGKFRAALPLVAIPTTSGTGTEVTQYSVLTDPLSGIKAGFGH
                     DLAFPRLAVCDPRHTLSLNPAVTLNTALDALSHLLEGIYSNKRDPLLYPLIHSGAKAI
                     RDNLQAVLDNPDGLPGRTELMRASLYGGIVIAQTSTTLQHSLGYPLTTAFGLPHGQSN
                     AMVMRQVLELYYPALEAELNALFRALGGSRKDFEAWLGELPFGRKLELDDAFIKKSVG
                     EVLASRNMANNPIEVSADQIRDIYRGLK"
     gene            2914..3858
                     /locus_tag="PLB85_04645"
     CDS             2914..3858
                     /locus_tag="PLB85_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU72652.1"
                     /translation="MTTFPRAGSEGTLIYPVFLGMRGCPGRCVYCDQDKLAGSGDFDP
                     EQAVEGVRKFVERHRGRAKQVAFYGGTFTAWEEHFREGLLRAVMAVCDVSTSFRISTH
                     PLYVSDEILAHCKRWNIRTIELGVQDFNDVVLAASGRGYSGREALEAALRVKNQDFEL
                     GVQLMPGLPGWTEASLSENRKALAELRPHLLRLYPLIVIRGTALEKLYNSGAYLPLSL
                     EEAVSQCADYHPVAESAGIRIIKEGIPSNIDPAEIAGGPWHPAFGELVKAELVARKVL
                     AIDPQEQAVNISRDEIRLLKAHGGAALKKIQDWFETRS"
BASE COUNT          764 a         1133 c         1133 g          839 t
ORIGIN      
        1 tcggcggatt tcactgcttc gatggtgccg cagggctctc cggcggaaac ctttgccccg
       61 gcgtcgggca gttccaaaaa gacgatatcc cccagttcat gctgggcaaa atcggtgatg
      121 ccgatggtgg cgatgccgtc tttgacgctc acccattcgt ggctctcggt gtagtatagt
      181 tcatcgggta caagcattgt atcctcctgt atttgtgctt tgaaagctgt tccacgccaa
      241 atcccagtat ggcgtttttg tgtcaagcac aagtttgccc gctcgggctg ccattaattt
      301 actgatcagg gcttgggcga atcccatttt ttcgggcggg gatattccgc ttgaccaaaa
      361 ttgtcgccgc cgcctgaatg gcgcaactcc ctgtccgagg atagatatga ctacaaacac
      421 agcaagcaag aggatcctgg cggtccacga cctttccagc ttcggccaca cttcgctgat
      481 ggccttcatc cccatcatgt atcggctggg gatcagggtt tgcgccctgc ccacggccat
      541 cctttccgcc aatacggatt tcccaaaccc gctctggatc gacttcagcg gccaaattga
      601 gcccttcgcg gatcactggg aggcgctgga actgcgtttc gacgccgtca gcacaggttt
      661 cctggcttcg gccgaacagg cggaactggt gggcaaagcc atcgaccggc tgaagcagct
      721 cggaaccctg gtggctgtgg atcccgtgat gggcgaccag ggcagccttt actcctgttt
      781 cggccctccc ctcgtggatg ccatgcgccg cctggtggca aaagcggaac tcatcacccc
      841 gaatttcacc gaagccgcgc tgctggccgg ggccgacccc actccacggg cagagccgga
      901 tgaggtgagg cgctggtgcc aggccattgc cgcttctggg ccggaacaaa tcgtggtgac
      961 ctccgttccc accgccgacc ccagtcaact tgaagtgctg cattacaacg ctcccgagga
     1021 cagcctcagc cgccttccct tcgccgtgag cccaggccac aaacctgggg cgggcgactg
     1081 ctttgccgcg ctgctcctgg ccggactggt gaacgggcat tccctgccta cttctctcaa
     1141 ggcaacggtg aggatcatga gccgcgccat cctggaagag cttccccccg gcgccgactg
     1201 gagggagggc attgccatgg aaaacatgat acagtgggac cttcaggctt attataggga
     1261 gtaaaagtga aaaccatcat cctcctcacc atttccaata tcttcatgac tttcgcctgg
     1321 tatggccacc tcaaatacaa gaacaccccg ctcttcactg tgatcctcat ctcctggggt
     1381 atcgctttct ttgagtattg cttccaagtg ccggccaacc gcatcgggca cggggtcttc
     1441 aacgcctacc agctcaaaac catccaagag gtcatcacgc tgctggtttt cagcgtcttt
     1501 tccgtgcttt acctcaagga ggaattccgt tggaactatc tggtgggctt cctgttcctc
     1561 attggggcgg tgtttttcat tttcaagaag tggtgagccg ccgtcacttc aggccgcggt
     1621 agatatccct gatttggtct gcgcttacct cgatggggtt gttcgccatg ttgcggctgg
     1681 ccaaaacctc gcccacgctc ttttttatga aggcgtcgtc cagctccagt ttcctgccga
     1741 aaggaagttc ccccagccag gcctcgaaat cttttcggct accgcccaaa gccctgaaga
     1801 gggcgttcag ttccgcttcc agggcgggat aatagagctc cagaacctgg cgcataacca
     1861 tggcgttgct ctgtccgtgg ggcaggccaa acgctgtggt gagcggataa ccaagcgaat
     1921 gctgcagggt ggtgctggtc tgggcgatga cgatgcctcc gtagagcgag gccctcatca
     1981 gttccgttct tccgggcaag ccgtccggat tgtccagaac ggcttgcaga ttgtccctga
     2041 tggctttggc gccgctgtgg atgagggggt aaagcagggg gtcgcgcttg tttgaataaa
     2101 tgccttccag caggtgggaa agtgcgtcca gggcggtgtt gagggtaacg gccgggttca
     2161 ggcttagcgt gtggcgcgga tcgcaaaccg ccagccgggg aaaggccaga tcgtgcccaa
     2221 agccagcttt gatgccgctg agggggtcgg tgagcacgga atactgcgtc acctccgtcc
     2281 cggttccgga agtggtggga atggccacca ggggcagggc tgccctgaat ttgcctgtct
     2341 ggtagatttc gtcgcgcccc aaggaattcg ccgccgccag ggaaatggct ttcgccgcgt
     2401 cgatggggct gccgccgccg atgcttatca gaaagtcgca gccgctttcc cgcagcagcc
     2461 tggcccccgc cagaacggta tccaggctgg ggttttcctc cacctggtca aaaagttccc
     2521 aagccagacc ggccctgtcc aataccaggc tgagctcgtc caaagcgccg ctgagtctgg
     2581 cgctggaacg ccccgtgaca actaggggtc ggctacccag gctcaaaagc tggtccgccg
     2641 ctttcagcag ggcgccctcg ccgaaaaaga ttcgtgtggg aagatggatc ataatgtgta
     2701 cctcgctgtg gcgtttctaa gctttcgcac ctttgctgtc aaggtaattc tggcagcaat
     2761 ccccaaaaaa ggctctcttt ccaatcaggc gggtgatccg gctactctag cccagagggc
     2821 ttaccatccg tattgaaaca aagcctgaaa aacatattgc cggcattggt ctggattttg
     2881 ctcaatatat ttgacaactg agggaggtta caaatgacaa catttccccg ggcaggttct
     2941 gaaggaacgc tgatctatcc ggtgtttctg ggtatgcgcg gctgtccggg cagatgtgtg
     3001 tactgcgacc aggacaagct tgccggcagc ggggatttcg acccggagca agcggtggag
     3061 ggggtacgta agtttgtgga gcgccatcgg ggccgggcca agcaggtggc cttttacgga
     3121 ggcactttca ccgcctggga agagcatttt agagaagggc tgctgcgcgc ggtgatggcg
     3181 gtttgtgacg tctccaccag tttccggatt tccacccatc ccctttatgt gagcgacgaa
     3241 atcctggccc actgcaagcg gtggaatatc cgcaccatcg aactgggggt gcaggatttt
     3301 aacgatgtcg tgctggctgc ttccggccgg ggctacagcg gcagggaggc cctggaagcc
     3361 gcgctcaggg tgaaaaacca ggattttgaa ctgggcgtgc aactgatgcc cggcctgccg
     3421 ggatggacgg aagccagcct gtcagagaac cggaaagccc tcgcagaact gaggccccac
     3481 ctcctgcggc tctatcctct catcgtgatc cgcggcaccg ctttggaaaa gctttacaat
     3541 tccggcgctt accttcccct cagcctggaa gaggccgtca gccagtgcgc ggattaccat
     3601 cccgtggccg agtcagccgg aattcgcatc atcaaagagg ggattccctc gaacatcgac
     3661 ccggccgaaa ttgccggagg accctggcat cccgccttcg gcgaactggt gaaggcggaa
     3721 ctggtggcca ggaaagtgct ggctatcgac ccgcaggagc aggcggttaa tatcagccgc
     3781 gacgaaatcc gacttctcaa agcccacggc ggggcagccc tgaaaaaaat ccaggattgg
     3841 tttgaaacgc gctcctgaac tggctttgg
//
LOCUS       DAOOWR010000156         3823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_23972_length_3823_cov_3.437898, whole genome shotgun sequence.
ACCESSION   DAOOWR010000156 DAOOWR010000000
VERSION     DAOOWR010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23972_length_3823_cov_3.437898"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLB85_04650"
     CDS             <1..969
                     /locus_tag="PLB85_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOU72653.1"
                     /translation="PLCLNGDELGFSGEFVLKCVEGRLVLTHLLPLEDYVAGVIANEI
                     GSKAPLEAMKAQAVAARTHAVSLLLSNSHKKDGYDLCNATHCQVYKGKYRQNETVRLA
                     ASSTAGEILIREGRIADATYHSCCGGRTDSSKAIWDGAPLPHLGGVLCDEAAAGLNLA
                     DEADARRWILKETAEKCNSAWEALALAWGRQLKRRELAKNVELADIDHLVINRRGASG
                     RITDITFHGEKAVRLTSEYRIRQAFGGAKSSFFYIEGGFVTTDNGGVVIYPPETLSIK
                     GRGSGHGVGMCQVGALRMARAGDDHLQILSHYYPGTEISTQWMQHE"
     gene            962..1840
                     /locus_tag="PLB85_04655"
     CDS             962..1840
                     /locus_tag="PLB85_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOU72654.1"
                     /translation="MNDQLWRGTAFSGQFRVLAVSSTQTAQTMRDLHDLSPVNTLLLG
                     KMIPAVAMLSMDLKLAEGEVSLRVDGDGPLKGALVICSGSGDLRGYALEPRLWLDDAE
                     ANLFPVRALGTGTLSVIRGFPNKKPVTGTTVLSEGELALNLAHYFDQSEQIPSVVNLG
                     VLIDKTAAVRASGGFIIQQMPDADPKLADELTAALSRTPNVSDLMDMGLSLPDILKRF
                     VVKESELELTPAGPIQYRCNCSRERFERALLLLGEEELSTMREGIEPQCHFCNATYCF
                     SAEDMNTLIATLKDKP"
     gene            1837..>3823
                     /locus_tag="PLB85_04660"
     CDS             1837..>3823
                     /locus_tag="PLB85_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOU72655.1"
                     /translation="MKHCLILVLLFAAALGAISTENFSWLLATENFDVLRKHSGKVEE
                     LICGPETEVRLALEFALRTQEPELELLCRERLALGHHSLADALEWLRLADQIKIDNAA
                     FEKVRQQLVEEFTSPDEQIVLDHYLYGTSDEDFLHELRHVRGYNPVIESLAREMIDRI
                     SVESSDSLALALIDTFERDYPLSEWGQVAYYYKLYHLSSVGAYDEMEALVQEKGFQSD
                     LHLYISAIYMLSPAYRRSRPDNSLLLNQVAYMLDSALKDYSREEKVRVLYDLLTPAEW
                     NTRLRFTRLKAEYYSLLAKHNLYGDETNLAALIPSAGPAFQQLLSDIQTLSFANNDRG
                     DLAELSYWQGRIEALPNQDFFLLRAARSFTRSLILGSPRRKYDEACLAALEELRQKLG
                     IEAGLITWARSLAGYRGIIFEEHPFPDKRYTRVAIGDYNNDGYNDLLFNGNALYRNEQ
                     GRNFVALSDSMNISRLNSNGGLWADFNLDGWLDFVAISHNSEGLGEALMKNQQGSRFV
                     KVNERAGEIDDRFPTEGAAWIDAGRTGYPSLYTANYETWQVQAGYPDFFWQNNDGYFS
                     DKSVELGFRTPGYTREPGLAGRGVAPADYDNDGEQEILVTNYRLTRNFCWDHADSLYL
                     DVAARDGLSGRYKDGWYGHSIGADWGDFDNDGDLDLFI"
BASE COUNT          843 a         1144 c         1090 g          746 t
ORIGIN      
        1 cccctctgcc tgaatgggga tgaacttggc ttcagcggag aatttgtgct gaaatgcgtg
       61 gagggcaggc tggtgctaac gcatctgctt cccctagaag attacgtggc cggggtgatc
      121 gccaacgaga tcggcagcaa ggcgcctctg gaggcaatga aagcccaggc ggtggcggcc
      181 aggacccacg ccgtgagcct gcttctgagc aacagccata aaaaagacgg ctacgatctc
      241 tgtaacgcca cccactgcca ggtttacaaa ggcaaataca ggcagaatga gacagtccgt
      301 ctggctgcca gttccaccgc aggcgagatt ctgatccgtg aaggccgcat cgccgacgcc
      361 acctaccaca gctgctgcgg cggcagaacc gactcctcca aagccatctg ggacggggct
      421 cccctacctc acctcggcgg agtgctgtgt gatgaggcgg ccgcaggttt gaaccttgcc
      481 gatgaagcgg acgcgaggcg ctggatcctg aaagaaacag ccgaaaaatg caacagcgcc
      541 tgggaagctt tggctctggc ctggggcaga caactgaagc ggagagaact ggccaaaaac
      601 gtggaactgg ctgacatcga ccatctcgtg atcaaccgcc gcggagcttc cgggcggatc
      661 acagacatca cctttcacgg tgagaaagct gtgcgactga ccagcgaata cagaatccgc
      721 caggcttttg gcggtgccaa atcatccttt ttctacatcg agggcggttt cgtgacaaca
      781 gacaacggcg gggtggtaat ctatcccccg gaaaccctct ccatcaaagg ccggggctcg
      841 ggacacggcg tgggaatgtg ccaggtgggg gctttgagga tggcccgggc cggcgatgac
      901 catctgcaga tactttcaca ctattacccg ggcacggaaa tctccaccca atggatgcaa
      961 catgaatgac caactttggc ggggaaccgc tttttcaggg caattccggg tgctggctgt
     1021 gtcgtccacc caaacggccc aaacgatgcg cgacctgcat gatctttctc cggtgaacac
     1081 gctgctgctg ggcaaaatga tccctgctgt ggcgatgctg agtatggacc taaaactggc
     1141 tgagggcgag gtttcgctgc gggtagatgg ggacgggccg ctgaaaggtg ctctggtgat
     1201 ctgctccgga agtggcgacc tgcgtggcta cgctttggag ccccggctgt ggctggacga
     1261 cgccgaagcc aaccttttcc ccgtacgcgc actgggaacc ggcaccctga gcgtgatccg
     1321 cggctttccc aacaaaaaac cggtgaccgg caccactgtc ctctcggaag gcgaactggc
     1381 gctcaatctg gcacattatt tcgaccagtc ggagcaaatt ccctccgtgg tgaacctcgg
     1441 cgtgctgatc gataaaaccg ccgccgtgcg ggcttccggg ggcttcatca tccagcagat
     1501 gccggatgcc gatcccaaac ttgccgacga actgaccgcc gctctgagcc ggacacccaa
     1561 cgtttcagac ctgatggaca tgggcctctc cctgccggat atcctcaagc gctttgtggt
     1621 gaaggaaagc gagctggagc ttacgccggc tggccccatc caatacagat gtaactgctc
     1681 gcgggaacgc tttgaacggg ctttgctgct tcttggcgaa gaagaactct ccaccatgcg
     1741 ggaaggaatc gaaccccagt gccatttctg caacgccacc tactgcttca gtgctgagga
     1801 tatgaacacc ctcatcgcga ctttgaagga taagccatga aacactgtct tatcctcgtc
     1861 ctgctgtttg ctgcggcgct tggcgctatc agcaccgaga atttctcctg gttgctggcc
     1921 accgaaaact tcgatgtatt gcggaagcac tccggcaagg tggaggaact gatctgcggg
     1981 cctgaaacgg aagtccgcct ggctttggaa ttcgcgctac gaacccagga accggaactg
     2041 gaactgctct gccgcgaacg gctggctttg ggccatcact ccctggctga cgcgctggaa
     2101 tggctccgtc ttgccgatca aatcaaaatc gacaacgctg ctttcgaaaa agtgaggcag
     2161 caactggtgg aagaattcac ctcaccggac gagcaaatcg tactggacca ctatctctac
     2221 gggacatctg atgaagactt tttgcatgag ctgcgccatg ttcgcggcta caatccagtg
     2281 atagaaagcc tggccaggga aatgatcgac cggatcagcg tggaaagttc cgattcgctg
     2341 gctttggccc tcattgacac tttcgagcgc gactatcccc tctccgagtg gggccaggtg
     2401 gcctattact acaagcttta ccacctttcc agcgtcggcg cctacgacga gatggaagcc
     2461 ctcgtccagg aaaagggatt ccaatccgac ctgcatcttt acatttccgc catctacatg
     2521 ctcagtccag cctacaggcg ttcccggccg gacaactccc tgctgctcaa ccaggtggcc
     2581 tacatgctcg attccgccct gaaagactac tcacgcgaag aaaaggtgcg cgtgctttat
     2641 gaccttctca ccccggcaga atggaacacc cggctgcgct tcaccaggct caaggctgaa
     2701 tactactcgc ttctggccaa acacaacctc tatggcgacg aaacaaacct cgccgcgctg
     2761 attccctcgg ccggacccgc cttccagcag ctcctcagcg atatccagac cctttccttc
     2821 gcgaacaacg accggggcga cctggcggaa ctgagctatt ggcagggcag gatcgaagcg
     2881 ttgcccaacc aggatttctt tcttctgcgg gctgccagga gcttcacgcg gtcgctgata
     2941 cttggttcgc cgcgccggaa gtatgacgag gcctgccttg cggccctgga agagctacgc
     3001 cagaagctgg gcatagaggc cgggctgata acctgggcgc gttccctggc cggttaccgg
     3061 ggcatcattt tcgaggaaca tcctttcccg gataagcgct acaccagggt ggccatcggc
     3121 gactataaca acgacggcta caatgatctg ctcttcaacg gcaacgccct gtaccggaat
     3181 gaacaggggc ggaacttcgt ggccctgagc gattccatga acatcagccg gctcaattcc
     3241 aacggtgggc tttgggcaga tttcaacctc gacggctggc ttgatttcgt ggccatttcc
     3301 cacaactccg aaggcctcgg cgaagccctg atgaagaatc agcaaggcag ccgcttcgtg
     3361 aaggtaaacg agcgcgccgg cgagatcgat gaccgttttc ccaccgaagg cgccgcctgg
     3421 atcgacgccg gccgcacagg ctatccctca ctttacaccg cgaactacga aacctggcag
     3481 gtccaggccg gatatcctga ctttttctgg caaaacaacg acggctactt cagcgacaaa
     3541 agcgtggaac tgggtttccg aacacccggc tacaccaggg aaccgggcct ggcgggacgg
     3601 ggtgtggcgc ccgctgatta cgacaacgac ggagagcagg aaatcctggt gaccaattac
     3661 cgcctcaccc gcaatttctg ctgggaccac gccgacagcc tttatttgga tgtggcagcc
     3721 cgggacggcc tgagcggacg ttacaaggat ggctggtatg gccacagcat cggcgctgac
     3781 tggggggatt ttgacaacga cggcgacctc gacctcttca tcg
//
LOCUS       DAOOWR010000157         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24171_length_3800_cov_3.594126, whole genome shotgun sequence.
ACCESSION   DAOOWR010000157 DAOOWR010000000
VERSION     DAOOWR010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24171_length_3800_cov_3.594126"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLB85_04665"
     CDS             complement(<1..202)
                     /locus_tag="PLB85_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU72656.1"
                     /translation="MPSIADNLNRVREKIEQTLQRCGRTGSEVTLVAVTKTHSVETVD
                     EALSLGIRHIGENKVQEALRKIP"
     gene            434..2905
                     /locus_tag="PLB85_04670"
     CDS             434..2905
                     /locus_tag="PLB85_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72657.1"
                     /translation="MKKILVLLVGLVALSIPVFRYHQHFNTHELSPGQTIRRKNVSSR
                     WIFADLAGTNYDYMLSAAPQGKNTYMLQVRDQIGKDISQINYSHPLRGITVLSDPRSK
                     APWMFLSINDQKATVVHGFHYIWEPMLKREERQFDAIARTDTLIAYEDYDWSGTLHPK
                     LLEDIDNDGSPELVCLAFDSFTINPRGLVVYDFDSGGLKWRFDLSTCISSLLCDDFDG
                     DGEKELVCGTIAYKNTDQEMRGMDDAHSWLMVIDARGRLLHHEMVNEGFSQVLLASDD
                     MDGDAQKEILAVCSTKGNAELPNSVKWLNWTGKRFIQKESWLLHGNLEFNNPETIYSL
                     MDGEGRKLVLLAAMNSPLIVLDSQLNKVNHGFNEPVSSVWGVEDLDLDGRKEILLETR
                     DDRLVVLSSDLKSKAELANPFNLDDNYSVHIVYTGFGKPPKIALAIGAEVRYYQYRRL
                     PLWEQVTRFIWLNLDYLCLILLLALLLLLIYVYRRRRIIMMDINNLGQGMVLMASKDR
                     ILHINDYMLDFLKDEYGNLPPGNLKSLSRLYPDLAALIPDFEASKASVFNQTMLLGRQ
                     QMRHNVQIQKLGGVTSKFLITAQPDLPAPGDAAATLAWADTARRLSHNVRRHITNIIL
                     ALKPLQTGGLDDKQLGYTDIIRSEIEKIRIFTHAFQRFTELKDYELKLQDVIPSLEHC
                     LERLTIPTGIKLIKNWDLASVEAWIEPIRFEEALGNVIANALDAMVEGGILHLSVKKF
                     PNHSGLNGRQSVMIEVEDSGKGIPAKYLEEVWQPFFTTKNDGTGIGLPETRKIITSMG
                     GTVLVQSEEGVGTVVTFWLKGSADG"
     gene            2898..>3800
                     /locus_tag="PLB85_04675"
     CDS             2898..>3800
                     /locus_tag="PLB85_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU72658.1"
                     /translation="MDKTRILLADDDSVFCRLVGDLLQQNGYEVTLALDVQGARQALQ
                     HSVFDIMMLDLCFPALMDGFNLLDEVRERYPSITVLMISGSGHIPDVVRAIKQGAYDF
                     IEKPIEPEHLLLRIGNLNTKILQERQMRHLEQAAIGMVGVSAQMGKVFSAISAAANFD
                     SPVLVTGETGVGKELAVRAIHRLSKFGTQDLMSINCASVPKELFEAELFGYEKGAFTG
                     AEKAFKGYFEFARNSSLFLDEVGELPAVVQAKLLRVLSEGEIQRIGGKVGISNARILS
                     ASNQDLQAAIQAGTFREDLYYRLNT"
BASE COUNT          904 a         1024 c          981 g          891 t
ORIGIN      
        1 ttgggatttt gcgcaaggct tcttgcacct tgttttctcc gatgtgccgg attcccaagc
       61 tcaaagcctc atcaacagtt tccaccgaat gggtttttgt aacggctacg agtgtcactt
      121 cagagccagt tcgcccgcat ctctgcagcg tttgctctat cttttcccga accctgttca
      181 gattgtccgc aatggagggc attcaggctc ctaatcttat ttgtgtattt ttatcaacaa
      241 tccaggcgcg ttcccaaaac cgactgggat aatgatccag cgcgcatcca gattaacttt
      301 cctatttttg ttgtggcgca tctctgtcaa gcagaatctc ccgttggcat ccaacaccga
      361 aaactgacca attttttact tgacaggctg agactaagat tagtattgca caaaccgatt
      421 tatttgggga agtatgaaaa agatattggt tcttttggtt ggactggtcg ctctgagcat
      481 tccagtgttt cgttaccacc aacatttcaa tactcacgag ctgagccccg gccagactat
      541 ccgcaggaag aatgtatcct ccagatggat ttttgccgac cttgccggta ccaactatga
      601 ctatatgcta tccgcagcgc cgcagggcaa aaatacctac atgctgcagg tgcgggacca
      661 gatcggcaaa gacatctcgc agataaatta ttcccatccc ctgcgcggca tcaccgtgct
      721 ctccgacccc cgcagcaaag ccccgtggat gtttctcagc atcaacgacc agaaagcaac
      781 agtcgtccat ggttttcatt acatctggga accaatgctc aagcgcgagg aaagacaatt
      841 cgacgccata gcccgcacgg atactttgat cgcctacgaa gactatgatt ggagcgggac
      901 cctgcatccc aaattgctcg aggatatcga taatgacggc agtccggaac ttgtttgcct
      961 ggccttcgat tcctttacca taaacccccg cggcctagtg gtctatgact tcgacagcgg
     1021 cgggttgaaa tggcgcttcg acctcagcac ctgcatctct tccctgctct gtgacgattt
     1081 tgacggggat ggcgaaaagg aactggtctg cggaaccatc gcctataaaa acactgacca
     1141 ggaaatgcgg ggcatggacg acgcccattc ctggctgatg gtgatcgatg cccggggccg
     1201 gcttcttcac catgaaatgg ttaacgaagg cttcagccag gtgttgcttg cttccgacga
     1261 catggacggg gatgcccaaa aagaaatcct ggccgtttgc tccaccaaag gcaacgccga
     1321 gctgcccaat agcgtgaaat ggctgaattg gacaggaaaa cggtttatcc agaaggaaag
     1381 ttggttactg cacggcaatc tggaattcaa caaccccgaa accatataca gcctgatgga
     1441 tggggaaggt cgcaagcttg tccttctggc agctatgaat tcacccctga tcgttctcga
     1501 cagccagctc aataaggtta accacggctt taacgagcct gtgagcagtg tttggggtgt
     1561 tgaagatctc gatctggacg gccgcaagga gattctgctg gaaacccggg acgaccgtct
     1621 tgttgttctg agcagcgatc tcaagtccaa agctgagttg gcaaaccctt tcaaccttga
     1681 tgacaattac tccgtccaca tcgtgtacac cggttttggc aagccaccca agatcgcgct
     1741 ggccattggg gctgaggttc gttattacca ataccggcgc ctgccgctgt gggaacaggt
     1801 cactcgtttc atctggttga acctggacta tctgtgtctt atcctgttgc tggcattact
     1861 gcttctgctg atctatgtct accgccgccg ccgcattatt atgatggaca tcaacaatct
     1921 ggggcaggga atggtcctga tggcttccaa agacaggatc ctccacatca acgattatat
     1981 gctggacttt ttgaaagacg aatacggcaa tctgcctcct ggtaatctga aatcgctttc
     2041 ccgcctctat ccggatttgg ctgctctgat acccgatttc gaggccagca aagcttcggt
     2101 ttttaaccag accatgctgt tgggccgcca gcagatgcgt cacaacgttc aaatccagaa
     2161 actgggaggg gtgaccagca agtttctgat cacggcccaa cctgacctcc ccgcgccagg
     2221 tgatgccgcc gccaccctgg cctgggcaga taccgcgcgc aggctttccc acaatgtaag
     2281 aaggcatatc accaatatca tcctggccct gaagcccttg cagactggtg ggttggacga
     2341 caaacagctt ggatacactg atatcatccg cagcgagata gaaaagatcc gcatcttcac
     2401 tcacgccttc cagcgtttta ccgagctgaa ggactatgag ctgaagttgc aggatgttat
     2461 cccctctttg gagcattgcc tggaacgcct caccatacca accggcatca agctgatcaa
     2521 gaattgggac cttgcctcgg tggaagcctg gatcgagccg atccgctttg aagaagcgct
     2581 gggaaacgtg attgccaacg cgctcgacgc catggtggag ggcggaatcc tgcatctgag
     2641 cgtgaaaaaa ttccccaacc actctggcct caatggaagg cagagcgtga tgatcgaggt
     2701 ggaggacagc ggcaaaggca ttcctgccaa atacttggag gaggtttggc agccattctt
     2761 caccaccaaa aacgacggca ccggaatcgg cctgcccgaa accagaaaga tcatcacctc
     2821 gatgggcggc actgtgctgg tgcagagtga agagggggtg ggaacggtcg tcactttttg
     2881 gctaaaggga agcgctgatg gataaaacca ggattctatt ggctgacgac gactccgttt
     2941 tttgccggct ggtgggtgac ctgctgcagc aaaatggcta tgaagtcaca ctcgccctgg
     3001 atgttcaggg agcgcgccag gccttgcagc acagcgtttt cgacatcatg atgctcgacc
     3061 tctgctttcc cgcgctgatg gatggtttca acctgttgga cgaggttcgg gaaagatacc
     3121 cttcgataac cgttctgatg atttcaggct cgggccatat tcccgatgtc gtgcgcgcta
     3181 tcaaacaggg agcttacgat ttcatcgaaa agccgatcga gccggaacat ctgctgctgc
     3241 gcatcggaaa cctgaacacc aagatattgc aggaaaggca gatgcgtcac ttggagcaag
     3301 ctgccatcgg tatggtgggg gtatccgcac agatgggaaa agttttctcc gccatcagcg
     3361 ccgcggccaa tttcgattcc cccgtgctcg tgactggcga aaccggtgtg ggcaaggaac
     3421 tggctgtgag ggcgattcac cgcctgagca aattcggaac ccaggacctc atgagcatta
     3481 attgcgcttc cgtgcccaaa gaactcttcg aagctgagct tttcggctat gaaaaaggcg
     3541 cttttacagg ggctgagaaa gcctttaagg gctattttga atttgcccgg aactcctcac
     3601 tttttctgga tgaagtgggg gaattgccgg ctgtggttca ggccaaactg ctccgcgttc
     3661 tttccgaagg ggaaatccaa aggatcggag gcaaggtggg aatctccaac gcccgcatcc
     3721 tgagcgcctc caaccaggac ttgcaggccg caatccaagc cggaactttt cgcgaagacc
     3781 tctattaccg cctcaacacc
//
LOCUS       DAOOWR010000158         3783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24305_length_3783_cov_3.773337, whole genome shotgun sequence.
ACCESSION   DAOOWR010000158 DAOOWR010000000
VERSION     DAOOWR010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24305_length_3783_cov_3.773337"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..812
                     /gene="pyrE"
                     /locus_tag="PLB85_04680"
     CDS             21..812
                     /gene="pyrE"
                     /locus_tag="PLB85_04680"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU72659.1"
                     /translation="MDPVLVTIAKFRDNFEADLAREFLADNGIQAFLQNELVYSMLPG
                     ILPGKFDIELQVASSDEAHARELLAQQQNTPEIRNILIANGAILEGHFLLTSGKHSGT
                     YVEKIRLLQDPAAAAKVCRLLAELLEPFDFDTVVGPAYGGIVLAFEVARLLGRNFIFS
                     QRKDERMTIRSGFDLSGVKKAVVVEDIITTGGSVREVIACLSERGIEVQSVAGIVDRS
                     GGEADFGCPFLSLLKMDIPAWNAESCPLCASQVPLTRPGASDKLL"
     gene            817..2289
                     /locus_tag="PLB85_04685"
     CDS             817..2289
                     /locus_tag="PLB85_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU72660.1"
                     /translation="MNDIAVIALSRWERQQLAAIWKFFPFAADISWHYLYTGPEDPEE
                     LPIHPGFRPQAFSAADPVSVMRDHIEKEGITRLCFASLGTLRRWTLATGSSPITLYEG
                     ARLSLADLSALEEGFTARIDELCQALGRHPAILLAEAETLGQPKDGSVYADFEAFRRA
                     RYGELLCTGNRDLVSAVCHSSLPSFTVRHIGEGWQALNLALDEEKVDFLTALNRLRHA
                     SADAVENTPGCKSGSPSAYQTSYSLFAQYYDSYMSHVNYEQWVDLILSWHKRVAQETP
                     KRVLELACGTANASEILVFRGMEVDACDISPYMLHVADAKPFKPSLFLNSLTDPIPGG
                     GYDLIFCLFDSFNYLVKKADAIQLLNHAHKALKPGGTFVFDISTLKNSLENFCDTTSF
                     NRVRDGYLVQISTYEPLTYRQITHLLLFRKNLNAYERFEERHVQRVYRTPELVELCAA
                     SKLRLKAIFSPDTRPNLISRDGNDLDNRYFRLFFLLQKPL"
     gene            2286..2888
                     /locus_tag="PLB85_04690"
     CDS             2286..2888
                     /locus_tag="PLB85_04690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU72661.1"
                     /translation="MNALYAHDLGIANLQGRLIGVDEAGRGALAGPVVVAAVALDYSH
                     PIPDINDSKKLSPRRREQLFAEITSSASAWFIVEVDAAWIDSHDILQATLKGMREAVT
                     AIAAENDLCLIDGNQLPPGLICPANSLIHGDSLSACVAAASILAKVHRDRLMAGYEPQ
                     YPLYGFAQHKGYGTAKHLQAISDHGPCPIHRRTFAPLSQI"
BASE COUNT          785 a         1235 c          982 g          781 t
ORIGIN      
        1 cctcaatgta aggagcggaa atggatcccg tgcttgtaac catcgccaag ttcagagaca
       61 actttgaagc cgacctcgcc agggagttcc tcgcggacaa cggcattcaa gcttttttgc
      121 aaaatgaact ggtttactcc atgcttccag gcatcctgcc aggcaaattc gacatcgaat
      181 tgcaggttgc ctcaagcgat gaggcccacg ccagggaact tctggcccaa cagcagaaca
      241 cgcctgagat aaggaatatc ctcatcgcca acggcgccat cctcgaagga cattttttgc
      301 tcacctccgg caaacacagc ggtacctacg tggaaaagat caggctgctg caggatcccg
      361 ccgccgctgc caaggtctgc agactgctgg ccgaactttt ggaacccttt gatttcgaca
      421 ccgtggtggg ccccgcttac ggcgggatcg tactggcctt tgaagtggcc cggctgctgg
      481 gcaggaactt cattttcagc cagcgcaagg acgagcggat gacgatccgc agcggctttg
      541 acctctccgg ggtgaaaaag gccgtggtgg tcgaggatat aatcaccacc ggaggttccg
      601 tgcgtgaggt gatcgcttgc ctcagcgagc gcggaatcga agtccagtcc gtagccggaa
      661 tcgtggaccg ctccggcggc gaggctgatt ttggctgtcc cttcctgtcc ctgctgaaaa
      721 tggacatccc cgcctggaac gctgaatcct gcccgctttg cgcgtcccag gtcccgctca
      781 cccgtcccgg cgccagcgat aagctgctct gaagctttga acgacatcgc cgtcatcgct
      841 ctcagccgct gggagcgcca gcaactggcc gcgatctgga aattctttcc tttcgccgct
      901 gatattagct ggcattacct ttacacggga ccggaggacc cggaagaact gcccatccac
      961 ccaggtttcc gcccccaggc cttttccgcg gccgatccgg tgtccgtgat gcgtgatcac
     1021 attgagaaag agggaattac gcggctttgc tttgccagcc tgggcacact gaggcgttgg
     1081 acccttgcca caggatcatc ccccatcacg ctttacgaag gtgccaggct ctctttggcc
     1141 gatctgagcg cgctggaaga gggtttcacg gcgcggatag atgaactctg ccaggccctg
     1201 ggccgccatc cggccatctt gctcgccgaa gcggaaaccc tgggtcagcc caaagacggc
     1261 agcgtctatg cggattttga agccttccgc cgcgcccgct acggcgaact gctctgcacc
     1321 ggaaaccgcg atctggtctc tgccgtctgc cattcaagcc tgccctcgtt cacagtgcgc
     1381 catatcggtg aaggctggca ggcgctgaac ctcgcattgg atgaggaaaa ggtggatttc
     1441 ctgaccgccc tgaaccgcct gcgccatgcc tcggccgatg cggttgaaaa cactcccgga
     1501 tgcaaatcag gaagcccctc cgcgtatcag acctcctatt cgcttttcgc gcaatattac
     1561 gattcctaca tgtcgcacgt gaactacgaa cagtgggtgg acctgatcct gtcctggcac
     1621 aagcgcgtgg cgcaagaaac ccccaaacgc gttctggaac ttgcctgcgg aaccgccaac
     1681 gcctccgaga tcctggtttt ccggggcatg gaagtggatg cctgcgacat ttcaccctac
     1741 atgcttcacg tggccgatgc caaacccttc aaaccctccc tctttctcaa ttcgctcacc
     1801 gatcccatcc ccggaggcgg ctacgacctc atcttctgcc tcttcgacag cttcaactat
     1861 ctggtgaaaa aagccgatgc catccaactg ctcaaccacg cgcataaggc cctcaaaccc
     1921 ggcggcacct tcgttttcga catctccacc ctcaaaaaca gcctggagaa tttctgcgac
     1981 accacctcct tcaaccgcgt ccgtgacggc taccttgtcc agatttccac ttatgaaccc
     2041 ttaacctacc gccaaatcac gcatttgctc ctcttccgca agaacctcaa cgcttatgag
     2101 cgttttgagg aacgccacgt gcagagggtg taccggaccc cggaactggt tgagctctgc
     2161 gccgcttcca aactgcgtct caaggccatc ttctctccgg ataccaggcc caatctgatt
     2221 tcccgcgacg gcaacgacct cgacaaccgc tatttccgcc tcttctttct gctccaaaaa
     2281 ccgctgtgaa cgccctctac gcccacgacc tgggcattgc caacctccaa ggccgcctga
     2341 tcggggtgga cgaagccgga cgcggcgcgc tggccgggcc cgtggtggtg gcagccgtgg
     2401 cgctggatta cagccatccc atcccggata tcaacgacag caaaaaactc tccccccgcc
     2461 gccgggaaca gcttttcgcc gaaatcacct cctccgcatc ggcctggttc atcgttgagg
     2521 tggacgccgc ctggatagac agccacgaca tcctgcaggc cacgctgaaa gggatgcggg
     2581 aagccgtgac cgccatcgcc gcggaaaacg acctctgcct tatcgatgga aaccagcttc
     2641 cgccagggct tatctgcccg gcaaacagcc tgatccacgg agattcgctc tccgcctgcg
     2701 tcgcagccgc ctccatcctc gccaaggtcc accgggatcg cctcatggcc ggctacgaac
     2761 cccaatatcc gctctacggc ttcgcccaac acaagggtta cggaacggca aagcaccttc
     2821 aggccatttc agaccacgga ccctgcccca tccaccgcag gacttttgcg cccctttccc
     2881 agatttgacc gcgttttcag acacgcaatc ctgtctgtaa cccgccatcc cactttgcga
     2941 acaagcaaag cgatgcctac tggccgttaa ggccctgtcc cgtgttcctg aatcgcctcc
     3001 cgtatgatgc ccgcatttga tgcgggaatt gtgcgagtgg agtgcgaggg ggatggactc
     3061 gggcgctaag gacgcggcta gcaggattcg atggtggtat ggcccgcggg gcggaagaaa
     3121 gatagcgcgg gtgaggagaa aacgtagcga tcggatagca gactggagga ctttaggctc
     3181 tctttcaaag ctggtgggta gcccgaaggg cagcagaacg atagcgaggg tgtgaagtga
     3241 gcaaagcgaa cggaacccct cgacagcgat ttcccaaacc tatcaaagcc tcgcagaggc
     3301 gacagaaccc cggagtttga tttctgtcgc ccgcaagggg ctttccacct tgttgttgcc
     3361 cttgtacaat gaggttacgc accctcgcta tcgttatgtc gccctacggg cttgacctgg
     3421 aatccagaaa caaccaccgc ccagccccca acccacgtca ttccgggctt gacccggaat
     3481 ccagtccttt tcctgtcatg gatcccggat caatgtcacc tcgcgagctc ggtgacgtcc
     3541 gggatgacgt atgttcggct ctcccgacca atgtcttgca cgtcattcca ggcttgccct
     3601 ggaatccaga aacaaccacc gccagctccc aacccacgtc attccgggct tgacccggaa
     3661 tccagtccat ccctgatcca ttctcgtttt ccgcgttttt cggtgacgaa tgccccattt
     3721 gttacctgca atatggaggc tcaatttgcc ctgtcccact cttcagaatg ccgtcgcgga
     3781 ata
//
LOCUS       DAOOWR010000159         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24395_length_3773_cov_3.309575, whole genome shotgun sequence.
ACCESSION   DAOOWR010000159 DAOOWR010000000
VERSION     DAOOWR010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24395_length_3773_cov_3.309575"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /gene="glnA"
                     /locus_tag="PLB85_04695"
     CDS             <1..1194
                     /gene="glnA"
                     /locus_tag="PLB85_04695"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOU72662.1"
                     /translation="DPFFETPTLRFICSICDAETRIGVKKDPRSVALRAHEYLKSTGI
                     ADHSTWIPELEFHLFDTVEYNSDEFSSGYTVTSAECKDALPFDFEDDDAVAQQGRKGY
                     HMDTPFDRYYEIRQEMVDRMEEQGIKVRYHHHEVGLSSQQEIETELLEFPKICDDTMV
                     MKDIIRRTALEYGLTATFMPKPVYNQAGNGMHFHMMLHKNGKNIFYKKGGYADLSDEA
                     IWFIGGILKHGRALVAFTNPSTNSFKRLLPGFEAPVKLFYGLANRSAAIRIPKYTNTP
                     DTKRFEFRTGDATCNPYFAMSALLLAGLDGIINKIDPAKHKLGPYDDNVFAWSEKQKA
                     KLLPIPANLAEAMESLEKDHDFLLQGNVFNEELIESHIKHKIAEHHAVMSRVHPHEML
                     LYYNL"
     gene            1259..>3773
                     /locus_tag="PLB85_04700"
     CDS             1259..>3773
                     /locus_tag="PLB85_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72663.1"
                     /translation="MKHFLLLLLAGLAVSFAFAAASDQTPIQSGQAFAIMDKAASHTD
                     IRFTLPEFEIEQTEGGGVNYQRIRLPGSGSTLEDGLPELPTLSMNLAIPRLGGVEVRV
                     LSQEQSQIQQFLAYPVQQGLNEEAPKSFVIDSAFYTGGSSYPTSTVQWSDPMILRDFR
                     LVNVVVNPFSYDPQTKTLTVNQQIDLRISYTNAPGLNELEGELTSVSPSFAKIYESMI
                     LNFDDYRNLMYSNVPPRYLIIYGNNSDPAFVTALNEYVLWKKQKGADVDVASTASNEA
                     GNTTTTIKNYIQNAYNNPATRPDFVILLGDTSGSYTIPAYMVSNGGGDYPYTHLAGND
                     ILGDCFIGRISVENLSQLHNLLAKIYLYERDVNLNTADWLNRMLLVGDNAPSGISTMY
                     ISKYIKERALFVNPDYTFTEIYNGSPSTSEINAAINQGVGFYSYRGYIDFSPPAESAL
                     FNAYRLLHAINITCGSNNYWNGTSEMEQFIRYGTPAVPKGAVTGIGMSTSSTHTNFNN
                     VLHGGIFGGIFQYGMRTIGEALLNGKLYMSQTYGVASPSNVTNFTHWCNLMGDPTMET
                     WTGIPGHFNVTVIPSIPLGLSLMDVNVLDGTNLPVEGAAVTLTQNSSIISRGYTDTEG
                     NIILTLPQSMTPNNCVLTVSKHDFKPLQQTLTVDNSGTLVPASIVIDDDNSGASSGNN
                     DGLAHGGETLEILFGLTNTSANPISGLSGQVHTNSPWVTFVDSLVTYGEIAGSGLGFN
                     ASPVVLQIAPGAPDGAMIRIHVLLTDSQNQSYDISEFIPIHNARIMYNTSLVTNGGNQ
                     VLDPGETAGFTVTVSNTTATGVTDIWGKLFSLNDLVSVTD"
BASE COUNT          930 a         1167 c          927 g          749 t
ORIGIN      
        1 gatcctttct tcgagactcc caccctgcgc ttcatctgct caatctgcga cgctgaaacc
       61 cgcatcggcg tgaaaaagga cccccgcagc gtagccctga gggcgcatga atacctgaaa
      121 tccaccggca tcgcggacca cagcacttgg attccggagc tggaattcca ccttttcgac
      181 accgtggaat acaacagcga cgaattttcc agcggctaca ccgtaacctc cgcggaatgc
      241 aaggacgccc tgcccttcga ctttgaagac gacgacgcgg tggcccagca aggacgcaaa
      301 ggctatcaca tggatacacc tttcgaccgc tactacgaaa tccgccagga aatggtggac
      361 cgcatggagg agcaggggat caaggtgcgc taccaccacc acgaggtggg gcttagctct
      421 cagcaggaaa tcgagaccga actgctggaa ttccccaaga tctgcgacga caccatggtg
      481 atgaaggaca tcatccgccg caccgctctg gaatacggac tcacggcaac ctttatgccc
      541 aaacccgttt acaaccaggc cggaaacggc atgcactttc acatgatgct gcacaagaac
      601 ggcaagaaca tattctacaa aaagggcggc tacgcggacc tctccgatga agcgatctgg
      661 ttcatcggcg gcatcctgaa gcacgggcgc gcgctggtgg ccttcaccaa tcccagcacc
      721 aacagcttca agcgcttgct gcctggcttt gaagcgccgg tgaagctctt ctacggcctg
      781 gccaaccgca gcgcggccat ccgcattccc aaatatacca acacaccgga taccaagcgt
      841 ttcgaatttc gcaccggcga cgccacctgc aacccttact tcgccatgag cgccctgctg
      901 ctggcgggcc tggacggcat catcaacaag atcgaccccg ccaagcacaa actggggccc
      961 tacgacgaca acgtatttgc ttggagcgaa aagcagaaag cgaaactgct ccccatcccc
     1021 gcgaatctgg ccgaggccat ggaaagcctg gaaaaggacc acgacttcct gctgcaaggc
     1081 aacgttttca acgaagagct gatcgaatcg cacatcaagc ataagatagc tgaacaccat
     1141 gcggttatga gcagggttca tccccatgaa atgctgttgt attacaatct ctaaggcttt
     1201 ggaacacata ttgcttaatc cttaggcaaa ccatattcac acctaatgag gtaagttaat
     1261 gaaacacttc ctgctcctgt tgctggcagg gcttgccgtg agcttcgcgt ttgccgcggc
     1321 cagcgaccag acccccatac aatccggcca ggcctttgcc ataatggata aagccgcgag
     1381 ccataccgat atccggttta ctttgcccga gtttgaaatt gaacaaactg agggcggcgg
     1441 cgtgaactac cagcggatca ggcttcccgg ttccggaagc accctggaag acggcctgcc
     1501 cgaattgccc acactgagca tgaacctggc cattccccgc cttggcggag ttgaagttag
     1561 agttctaagc caggagcaga gccagataca gcagtttctg gcgtatccgg tgcagcaggg
     1621 cttgaatgag gaagctccca aatcctttgt gatcgattcc gccttctaca ccgggggttc
     1681 ctcttatccc accagcaccg tgcagtggag cgatcccatg atcctgcgcg atttccgcct
     1741 tgtgaatgtg gtggtgaatc ccttcagcta cgatccccaa accaagaccc tgaccgtgaa
     1801 ccagcagatc gatctgcgga tcagttatac caacgccccg gggctcaatg aactggaagg
     1861 cgagctgacc tcggtttcac catcctttgc caagatctat gaatcgatga tcctcaactt
     1921 tgacgattac cgaaacctta tgtacagtaa tgttccgccc cgttatctga ttatctacgg
     1981 caacaactcc gacccggcct tcgttacagc cctgaacgag tatgtgctct ggaagaagca
     2041 gaagggcgcg gacgtcgatg tagccagcac ggcatccaac gaggccggca acaccaccac
     2101 caccatcaag aactacattc agaacgctta caacaacccc gccaccaggc ctgactttgt
     2161 gatcctgctg ggcgatacct ccggcagcta taccatcccg gcctacatgg tcagcaatgg
     2221 cggcggggat tatccctaca cccatctggc tggcaacgac atcctcggcg actgctttat
     2281 cggccgcatc tcggtggaaa acctctccca actgcataac ctgctggcca agatttacct
     2341 ctatgagcgc gacgtcaacc tgaacacggc ggactggctt aaccggatgc tattggtggg
     2401 ggacaacgcc ccctccggca tatccaccat gtacatcagc aaatacatca aggaaagagc
     2461 gctgtttgtc aacccggact acactttcac ggaaatctac aacggctcac cctcaacctc
     2521 cgaaatcaac gctgccatca accagggcgt tggtttttac agctaccgcg ggtacatcga
     2581 cttctcaccg cccgctgaat cggcactttt caacgcctac cggctgctac acgcgataaa
     2641 catcacctgt ggctcgaaca actattggaa cggcacttcc gaaatggaac agtttatccg
     2701 ttacggcact ccggccgttc ccaaaggcgc cgtaaccgga atcgggatga gcacctccag
     2761 cacccatacc aactttaaca atgtgctgca cggagggatt ttcggcggta tcttccaata
     2821 cgggatgcgc acaatcggcg aagccctgct gaacggtaaa ctctatatgt cacaaactta
     2881 cggggtggct tctccgagca acgtgaccaa tttcacccac tggtgcaatc tgatgggtga
     2941 ccccactatg gaaacatgga ccggcatccc aggtcacttc aacgttacgg tcattcccag
     3001 cattcccctg ggcctcagcc tgatggatgt gaatgtgctg gacggaacca accttccggt
     3061 ggagggagca gccgtaaccc ttactcagaa tagctcgatc atcagcagag gctatacgga
     3121 cacagagggc aacattatcc ttaccctgcc gcagtcgatg acccccaaca attgtgtgct
     3181 caccgtttcc aagcacgatt tcaagcccct gcagcagact ctgaccgtgg acaacagcgg
     3241 caccctggtg ccggccagca ttgtgataga cgacgacaat agcggcgctt cctctggcaa
     3301 caacgacggt ttggcccacg gcggtgaaac actggagatc ctctttgggc ttaccaacac
     3361 ctccgccaac cccatctcag ggctaagcgg gcaggtccat accaacagtc cctgggtaac
     3421 atttgtggat tcgctggtta cctatggcga gatcgcgggc agcgggcttg gcttcaacgc
     3481 cagtcccgtg gttctgcaaa tcgcgccggg agcccccgac ggcgccatga tccggatcca
     3541 cgtgctgctt acggattccc aaaaccaaag ctatgacatt tcggaattca tacccatcca
     3601 caatgcccgg ataatgtaca acaccagcct ggttaccaat ggcggcaacc aggttctcga
     3661 tccaggcgag accgcgggct tcacggtaac tgtaagcaac accacagcca caggggtgac
     3721 tgacatctgg ggcaagcttt tcagcctaaa cgacctggtt tcggttaccg ata
//
LOCUS       DAOOWR010000160         3745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24619_length_3745_cov_3.878049, whole genome shotgun sequence.
ACCESSION   DAOOWR010000160 DAOOWR010000000
VERSION     DAOOWR010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24619_length_3745_cov_3.878049"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PLB85_04705"
     CDS             complement(<1..1585)
                     /locus_tag="PLB85_04705"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOU72664.1"
                     /translation="MHKHGILEYSQMRYEADRPKAANPIKIQDLSFRDGHQSLFATRG
                     RTEDLLHVAELMDQVGYYSMEVWGGATFDTMHRYLGEDPWERIRVLKKHIKNTPFSML
                     LRGQNLVGYQNYADDVVEAFVQRACDNGIDIFRVFDALNDFRNFQTAVKIIKKNNKHF
                     QGSICYSLTEQRMGGDIYNIEYYTKKAKQLEEMGADTVCIKDMAGLVAPYDAFNLITA
                     LKETVKVPVHLHTHFTSGMGDLALFKAIEAGVDIIDTCVGPYAYRTSHPAVEPFVIAL
                     LGTNRDTGFDIRLLNKIGKEMEKDIPKYLQFADNTKFNIIDTDVIIHQTPGGMLSNLV
                     NQLRQMDALDRLEDVFKEIPKVRKELGQIPLVTPTSQIVGIQAVNNALFDTKDGEYAR
                     ITEQVKDLCYGLYGKTTLPIDPEVQKKALKGYPRGEKPITVRPGDVIEPALEQVKKET
                     EGLAKDLDDALIVAIYNLTGKRFLKIKYGHEPLPAEMKATTLEEVKRQEELCRKAKAG
                     LLVEKPVKEPVPKPEGLRQF"
     gene            complement(1597..1785)
                     /locus_tag="PLB85_04710"
     CDS             complement(1597..1785)
                     /locus_tag="PLB85_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72665.1"
                     /translation="MTDRKKELAAIAGVLALISADESIPYPQRPLENAPSAWQHYGRQ
                     QTMLYRELSQRRMIKRNR"
     gene            complement(1794..3347)
                     /locus_tag="PLB85_04715"
     CDS             complement(1794..3347)
                     /locus_tag="PLB85_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOU72666.1"
                     /translation="MLSTRDAIQKLLDKKEKTLQMGGAKAVDKQKAGGKLTARERLDL
                     LFDPGTFRELDMFVRHRCDNFGMEKTEIASDGVITGHGLVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKVMDMALKSGVPLIGIQDSGGARIQEGVDSLKGYGEIFYRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRAVTGEETTQEELGGAM
                     THNSRSGNAHFACENDADAIQQIKILLSYLPANNMEDAPRTECGDDPWRLCPNLDSII
                     PDSPKESYSMHEVIKTVLDDGIFFEPHLYYAQNVIVGFGRLNGRVVGVVANQPKVLAG
                     CLDIDASDKATRFIRFCDAFNIPLLTFVDVPGYLPGTQQEHNGIIRHGAKLLWCYAEA
                     TVPKLTVVTRKDYGGSYIAMSSRHLGADMVLAWPSAEIAVMGAQGAANIIFRKEIEAA
                     EDKAAKRAEMIQIYEEEFNNPYVAASRGYVDAVILPSETRKRLIDALEILSSKSESLP
                     PKKHGNIPA"
BASE COUNT          652 a         1020 c         1128 g          945 t
ORIGIN      
        1 caaactggcg cagaccttcg ggcttgggaa ccggctcttt gaccggcttt tccaccagca
       61 ggccggcttt ggctttcctg caaagctctt cctggcgttt cacctcttcc agggtggtgg
      121 ctttcatctc ggccggcagt ggttcgtggc cgtattttat cttcaggaat ctctttccgg
      181 tgaggttgta gatagccaca atgagggcgt cgtccaaatc cttggccagg ccttcggttt
      241 ccttcttcac ctgctccagg gcgggttcga tcacgtcgcc ggggcgcacg gtgatgggct
      301 tttcgccccg cgggtaaccc ttcagcgctt ttttctgcac ttcgggatcg atgggcaggg
      361 tggtcttgcc gtaaaggccg tagcagaggt ccttcacctg ttcggtgatg cgggcgtatt
      421 cgccgtcctt ggtgtcaaag agagcgttgt tcacggcctg gatgcccact atctggctgg
      481 ttggagtcac cagagggatt tggcccagtt ctttgcgcac tttgggaatc tctttgaaaa
      541 catcttccag cctgtccaaa gcgtccatct ggcgcagttg gttcaccagg ttggagagca
      601 ttccgcccgg ggtttggtga attatcacgt cggtgtcgat gatgttgaat ttggtgttgt
      661 cggcgaactg gaggtatttg gggatgtctt tttccatttc cttgccgatc ttgttaagca
      721 ggcggatgtc gaagccggtg tcgcggttgg tgccaagcag cgcgatcaca aaaggttcca
      781 cggccgggtg cgaggtgcgg taggcgtagg gccccacgca ggtgtcgatg atatcgactc
      841 cggcctcgat ggccttgaac agcgccagat cgcccatgcc gctggtgaag tgggtgtgca
      901 ggtgcaccgg aaccttcacg gtttctttca gcgctgttat caggttgaag gcgtcatagg
      961 gcgccaccag gcccgccatg tccttgatgc agactgtgtc cgcgcccatc tcttccagct
     1021 gtttggcttt tttggtatag tattcaatgt tgtagatgtc gccgcccatc cgctgttccg
     1081 tgaggctgta gcagatgctt ccctggaagt gtttgttgtt ctttttgatg atcttcaccg
     1141 cggtctggaa attgcggaaa tcgttgaggg cgtcaaacac cctgaagatg tctattccat
     1201 tgtcgcaggc acgctggaca aaggcttcca ccacgtcgtc ggcgtagttc tggtagccca
     1261 cgaggttctg tccgcgcagc agcatggaaa agggtgtgtt cttgatgtgc tttttcagca
     1321 cgcggatgcg ttcccagggg tcctcgccaa gatagcggtg catagtgtca aaagtggcgc
     1381 cgccccacac ttccatggag taatagccca cctggtccat cagttcagcc acgtggagca
     1441 ggtcttctgt gcggccacgg gtggcgaaga gggattgatg cccgtcgcgg aaggacaggt
     1501 cctgaatttt gatcggatta gccgccttgg gacggtcggc ttcatagcgc atttggctat
     1561 attccaagat gccatgtttg tgcatggtgt ctccttctat ctgtttcttt ttatcattct
     1621 gcgttggctg agctcgcggt agagcatcgt ttgctggcgg ccgtaatgct gccaggcgct
     1681 gggggcgttt tccagcgggc gctggggata ggggatgctt tcgtcggcgc tgatcagggc
     1741 cagcactccg gcgatggcgg cgagttcttt tttccggtct gtcatggcgt tcctcaggcc
     1801 gggatgttgc cgtgtttttt gggcggtagg ctttcgcttt tgctggaaag tatttccagg
     1861 gcgtcgatga gccgcttgcg ggtttcggag ggtaggatca cggcgtccac atatccccgg
     1921 gaagctgcca cataggggtt gttgaattct tcttcgtaga tctgaatcat ctcggcgcgt
     1981 tttgccgcct tgtcttccgc cgcttctatc tctttgcgga agatgatgtt ggcggcgcct
     2041 tgggctccca tcacagcgat ctcggcgctg ggccaggcca ataccatgtc ggcgcccaga
     2101 tgccggctgc tcatggcgat gtagctgccg ccgtaatcct tgcgggtaac caccgtcagt
     2161 ttgggaaccg tggcctcggc atagcaccac agcagcttgg cgccgtggcg gatgatgcca
     2221 ttatgttctt gctgcgtgcc gggcaggtag ccgggaacgt ccacgaaggt gagcaggggg
     2281 atgttgaagg cgtcgcaaaa acggatgaag cgggtggctt tgtccgaagc gtcgatgtcg
     2341 aggcatccgg ccagcacttt cggttggttc gccaccaccc cgaccacgcg tccgttcagg
     2401 cgtccgaagc ccactatgac gttctgggca tagtaaagat gcggctcgaa aaagatgccg
     2461 tcgtccagca cggttttaat cacttcgtgc atggaatagc tttccttggg gctgtccgga
     2521 atgatgctgt ccaggttggg gcaaagccgc caggggtcgt ccccgcattc ggttctggga
     2581 gcgtcctcca tattgttggc gggcaggtag gagagcagta tctttatctg ctgaatggcg
     2641 tcggcgtcgt tctcacaggc aaagtgggcg ttcccgctcc tggaattgtg ggtcatagcc
     2701 ccgcccagtt cttcctgggt agtttcctcg ccggtgaccg cgcggatcac gtccggccct
     2761 gtgatgaaca tgaaactggt attcttcacc atgaacacga aatccgtcat cgccggggaa
     2821 tagaccgccc cgccggcgca gggccccatg atggctgtga tctggggaat aacgccgctg
     2881 gcgcgggaat tgcggtaaaa gatctcgccg tatcccttga gggaatccac gccttcctgg
     2941 atgcgcgctc cgccgctgtc ctggatgcca atgagcggaa cgccgctctt cagcgccatg
     3001 tccatcacct tgcagatctt ggcggcgtgc atttcgccca gggaaccgcc ccgggaagtg
     3061 aaatcctgcg aaaaggcgaa gaccggacgc ccgtccacca ggccgtggcc ggtgatcacg
     3121 ccgtctgagg cgatctcggt tttctccatg ccaaagttgt cgcagcggtg cctcacgaac
     3181 atatccaact cccggaaagt gccgggatcg aagagcagat caagcctctc gcgggcggtc
     3241 agtttgcctc cggccttctg tttgtcaacg gctttggcgc cgcccatctg cagggtcttc
     3301 tcttttttgt ccagaagttt ctggatcgcg tccctggttg atagcatgtt tatatcccct
     3361 ttctaaggtg tattttaagc tgtctgagcc ccggcctggc agccaggacc gactattttt
     3421 ccaaaatctt ttgccagcct cttttcgtca agaaaaagag cgccacgctt gcccggggaa
     3481 tcaagttact atgcatcgga acggtggaaa gagcgctaat ttcaccattt cgcccgacgc
     3541 gcggggaaca agcctgagcg agtttccgag ggaacggaca accgggattc agccaacaaa
     3601 tctggcaggt ttcacttgat actccatagc ttaaccgtcc gactgttttc cccggctgct
     3661 cccggttccg cttgacagga tgcggtattg cgtaaggtgt ggccacactc agaaacgcct
     3721 tggtttggag agaatacatg acagc
//
LOCUS       DAOOWR010000161         3736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24695_length_3736_cov_3.792991, whole genome shotgun sequence.
ACCESSION   DAOOWR010000161 DAOOWR010000000
VERSION     DAOOWR010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24695_length_3736_cov_3.792991"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PLB85_04720"
     CDS             complement(<1..1192)
                     /locus_tag="PLB85_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOU72667.1"
                     /translation="MRVAIGGFFHESNTFSPLVTGAEDFLIFRREEIYAHQDAYLLAK
                     GIISYFQAQAGYELVPLVFARAVPNGEVDAELYGRLKSEFFNYLQEAGPVDACVLALH
                     GSMRVRGIGSAESDLLLALKSAFPGLPVFCGLDMHATITRDLLRCSEALVGFKTAPHT
                     DAWETGWQTARLADLALSTKTRLRMGAARLDCLIAGEKSETDFEPMRSLIAELREIEA
                     FPEVCSASLILGFPWADAEENGVTALVVTQNDQALADACARRIRDSFSARRGEFRFSV
                     PAYPPEQALTLALRDTCKPVLVSDSGDNPTAGSTSDNTAVISLLANELKYLTRGKSIT
                     VAGIYDPEAVRVCAGRMGQRISLRVGGNFDTQYCQPLRLLGVPLRVVENFGLYAATLV
                     LFRTE"
     gene            1718..2314
                     /locus_tag="PLB85_04725"
     CDS             1718..2314
                     /locus_tag="PLB85_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU72668.1"
                     /translation="MANEIKGQKVTLRDLIDADLNDYRKWFTKGLEWTKWDAPWEEMS
                     DTFAQSFIQKLSERLSKGAQEKRMRFEIEVDGTHIGWVSSYFIENNPDFLAVGIVVPE
                     EDFWGKGIGKEALQLWVGYLFENPGLEYIYCETWSGNERMVRLALGVGFEIIEDGKVL
                     EHNGIKYHKLKFRIGNIRTSRQLRTGKEIDFCYPTPAE"
     gene            2467..3627
                     /locus_tag="PLB85_04730"
     CDS             2467..3627
                     /locus_tag="PLB85_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU72669.1"
                     /translation="MRKKWKIVIIIVAALLLLFLGLRQCGKAAQGKVAALGAAPSHKV
                     AKGEIVARVEITGELQPETVVSIKSKVSGRIVKLYADENDFVKKGDVIADIEPDYNQA
                     NTLFSTKAQLSRAELRLKNARDDLADKKILLDKGYIPRESYDAARDELSSAEIEFRQA
                     QSQYEAIQDMDVPGKVVHVIAPASGTVIERTVNEGEMVQSSLTSFGEGTVVMKIADLS
                     RMVVKSNINEVDIGKFHLGQEAKIKLDALPYEDFSGSIVKIAPAAIKENNAKVFPVEI
                     SINATGETAKPGLTAAVTIIGESRQNVLVIPIRAVFTDDKNQDIVYLKPKEDKAAKDQ
                     KNVPPSQPLPIPVTLGANDLQNVEVISGLKEGDEILLAEPPGQNNFMRMMMR"
BASE COUNT          914 a          914 c         1145 g          763 t
ORIGIN      
        1 cctccgtccg gaaaaggaca agggtggcgg cgtagaggcc aaagttttcc accacccgca
       61 gcggaacacc tagcagcctc agcggctggc aatactgggt gtcaaagttg ccgcctaccc
      121 ggagggagat tctttggccc attcgcccgg cgcagacacg gaccgcctcc ggatcataga
      181 taccggcaac cgtgatgctt ttgccgcgcg tgaggtactt cagttcgttt gccagcaacg
      241 agatgacggc ggtgttgtcg gaagttgagc cggcggtggg gttgtcgccg gaatcggaga
      301 ccagcacagg cttgcaggta tcccgcagag ccagggtgag agcctgttcg ggaggatagg
      361 ccggaacgga gaagcggaat tcgccgcgcc gggcgctgaa actgtcacgt atccggcggg
      421 cgcaggcatc cgccagggcc tggtcgtttt gggtgactac gagcgcggtg acgccgtttt
      481 cctccgcgtc cgcccaggga aagcccaaaa tcagggaggc cgagcagacc tctgggaatg
      541 cttcgatttc tcgcaattcg gcgatcaggg agcgcatcgg ctcaaagtcg gtttcggatt
      601 tttccccggc gatgaggcaa tccaggcggg cagcgcccat ccgcagcctg gttttggtgc
      661 tgagtgccag gtcggccagc cgggcggttt gccatccggt ttcccaggca tcggtgtgcg
      721 gggctgtttt gaaaccgacc aaggcttccg agcagcgcag aaggtcccgg gtgatcgtgg
      781 cgtgcatgtc caggccgcaa aagacgggaa ggccggggaa cgcggacttg agggcgagga
      841 gcaggtcgct ttccgcggaa ccgatacccc gaacccgcat cgaaccgtgc agggcgagca
      901 cgcaggcgtc cacgggcccg gcttcctgta gatagttgaa aaactcgctt ttgagcctgc
      961 cgtaaagctc cgcgtccact tcgccattcg gcaccgcccg ggcaaagacc agcggcacaa
     1021 gttcatatcc cgcttgcgct tggaaataag agatgatccc tttggcaagt aaataggcat
     1081 cctgatgagc gtatatttct tcgcggcgaa agatcaggaa atcctccgcc ccggtgacga
     1141 gggggctgaa agtgttagat tcgtgaaaga atccgccgat ggcgacacgc atgacattcc
     1201 tctgcagttg gttccttttg ggataaatct gtcggctgtg tgattatgtc aagcaggatg
     1261 tattcttttc ggagggagat cggccaataa tttttcaagg caggtaaagg ttggcagaga
     1321 atgaagcggc ggagacttgt ccgcttttgc ctgtcgctga caatctgaca gattcatcgg
     1381 gaggagccta tttcgctcga catgtttgac aaaatctggc cagagtgttt tattgtaaat
     1441 atccggcctc agggaggaaa aatgaaggcg gttcaaagct ttgtgatgtg cattgtcctg
     1501 ctcttcggag cggcggtttt gacagcgcag gcgcccgaat ggaagtgggg ggttgccgcg
     1561 agaggggttt ttcgcgttga aggccagtcc gtagccctcg acacagccag gttaaccagt
     1621 atgtcgccgg aaagtttgga gacacagccg ctttcggcag ccacaacctg acatgaagtg
     1681 gatacggaat accgatttgc tattggagag gtttaacatg gccaacgaga taaaaggaca
     1741 aaaggttact ctgcgagacc tgatcgatgc tgacttgaat gattatcgga aatggtttac
     1801 aaaagggctc gaatggacca aatgggatgc accttgggag gaaatgagtg atacatttgc
     1861 ccagagcttt atccaaaagc tatccgaaag actgtcaaaa ggagcacaag agaaaagaat
     1921 gcggtttgaa atagaagtgg atggcactca tattggttgg gtttcttctt acttcattga
     1981 gaataatccg gacttcttgg cagttggcat tgtcgtcccg gaggaagatt tctggggtaa
     2041 gggcataggc aaagaagcat tgcaactatg ggttggatac ctgtttgaaa accctggatt
     2101 ggaatacatt tactgtgaaa cttggtcagg taatgaaaga atggtgcgtt tggcccttgg
     2161 tgtaggtttt gaaatcattg aagatggcaa ggtgctggaa cataacggga taaagtatca
     2221 caaactcaaa ttcaggattg gaaatatccg cacctcaagg caactaagga ccggaaaaga
     2281 aattgacttc tgttacccga ctccagcgga ataaccgcaa tgctggagag tcttttccaa
     2341 ctgcgcggcg ctattttggc cccaagccct tcctctgccg ggagccggag ctttttcatt
     2401 gacagggaaa cagccgccaa atcagatgtc cggaaaaggc aaatagagat tggcggtaag
     2461 attcccatgc gcaaaaaatg gaagatagtc ataatcatag tggcggcatt gctgctgttg
     2521 tttctggggc tgaggcagtg cggaaaggct gcccagggca aggtggcggc tttgggcgcg
     2581 gctcccagcc acaaggtggc gaagggcgaa atcgtggcgc gggtggaaat cacgggtgaa
     2641 ttgcagcctg agacggtggt ttctatcaaa tccaaggttt cgggccggat cgtgaaactc
     2701 tacgcggacg aaaacgactt cgtgaagaaa ggggacgtga tcgcggacat cgagccggac
     2761 tataaccagg cgaacacgct gttcagcacg aaagcccagc tcagccgggc ggaactgcgg
     2821 ctgaaaaacg cccgggacga tctggcggac aaaaagatcc tgcttgacaa gggttacatc
     2881 ccccgggaaa gctatgacgc tgcccgggac gaactgagct cggctgaaat cgagtttcgc
     2941 caggcgcaaa gccagtatga ggctatccag gacatggacg tgcccggcaa ggttgtccac
     3001 gtcatcgcgc ccgcttccgg aacagtgata gagcgcacgg tgaacgaagg cgaaatggtg
     3061 cagtccagcc tgaccagctt tggcgaggga actgtggtga tgaagatcgc ggacctttcg
     3121 cgcatggtgg tgaagagcaa tatcaacgag gtggacatcg gtaaattcca tctggggcag
     3181 gaagccaaaa tcaagctgga tgcgctgcct tacgaggact tctccggctc gatcgtgaag
     3241 attgctcccg cggccatcaa ggaaaacaac gccaaggtct ttcccgtgga aatcagcatc
     3301 aacgccaccg gggaaacagc aaagcccgga ttgaccgcgg cggtgaccat catcggcgaa
     3361 tcgcgccaga acgtattggt gatccccatc cgcgcggtgt tcaccgacga caagaatcag
     3421 gatatcgtgt atctgaagcc caaagaggac aaggccgcca aagatcaaaa gaatgtcccg
     3481 ccaagccagc ctttgcccat cccggtgacc ttgggcgcca atgacctcca gaacgtggaa
     3541 gtgatctctg gtctgaagga aggcgacgaa atcctgctcg cggagccccc ggggcagaat
     3601 aattttatgc gcatgatgat gaggtaacga gatgatagag atcaaaggtc tgtcgcgcag
     3661 ctttggccag atcaaggccg tagacagggt tagcttcgct gtgaaaagcg gcgaaatagt
     3721 gggttttctg ggcccc
//
LOCUS       DAOOWR010000162         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24811_length_3722_cov_3.779111, whole genome shotgun sequence.
ACCESSION   DAOOWR010000162 DAOOWR010000000
VERSION     DAOOWR010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24811_length_3722_cov_3.779111"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2286)
                     /locus_tag="PLB85_04735"
     CDS             complement(<1..2286)
                     /locus_tag="PLB85_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72670.1"
                     /translation="MSNSKLVISIELSGKEKLEDIIEKLSKKYKLHADADFADIGTSL
                     KTQISALDDFKRRAKELGLVMNGISSAIGAVVGAYNATVRKFVESAAEFEQLQLRLES
                     LYQDADKAAAAFEKFRQVAAQTPATLKGVVEAGATLKAFGLDAENVLDSVADLAAYMG
                     MDIVEAAQAVGRAFAGGVGAADVLRERGVLELIKSFKGVEDLTKLTLPQFREAMLETF
                     SDAAAGIAGSSDRMAESYTGAVANMQDAMESLYAAIGNKITPIIGAAANALAKLADKL
                     SGTKSGLDAVKEGLVNQRVEFEKLVLVYKELHFRQNRSKEENEKYRKTINDLMTKYPN
                     YLGNIDLEKGAWEDIAAALNSARSKLQEWTNAKIKQAIIDDKTDDYVKLYKKLNEKEN
                     QLKTLQAEFEAGTKKREAYRQAAPDPLSSSPNMPRLILVSSKWAKQEEKLEKDIKKLK
                     AEIKKTDKEIDQLVSRLGEDTSLAPSNEESNKNKGKGKGSGKTAASKKTELDQVISEI
                     TEYRILRENKFNEEQTEIELLKREYTEKLKLVAKDKAATADLSEKRDREVGEIQKKYQ
                     EEREEEEAAHYEKLKFFAVGYYDWKLEQINKEAEDPNVPAAWKDEQIEQLNREREEWD
                     QRAIAAFEEKYDSEMSHLAELRDLGLATYGEIAAASWDYYGKLKEIVEGDGELTEAEK
                     ELLNIYLRRAQAAQLAVNRESDTARYYEEVKFLDANYYEWKKARIIEDVNELKLSLDQ
                     KEMLLAERLGELYQELAEFESQ"
     gene            complement(2444..2581)
                     /locus_tag="PLB85_04740"
     CDS             complement(2444..2581)
                     /locus_tag="PLB85_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72671.1"
                     /translation="MKLRYILYRIGAEPRRVELDEALQLAEIYAEEASARFCPFSGET
                     A"
     gene            complement(2578..2931)
                     /locus_tag="PLB85_04745"
     CDS             complement(2578..2931)
                     /locus_tag="PLB85_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72672.1"
                     /translation="MKIHTDVTTLSKLYPIFQEAGIEGMLTGDLEKIRGYGYPELFAA
                     LLQTGGIPEVCALITRSETCVQPASKEKKPWDQCSREETMAVILAFFFSLIGLQAESQ
                     HLMNALQTALRQISS"
     gene            complement(2933..3313)
                     /locus_tag="PLB85_04750"
     CDS             complement(2933..3313)
                     /locus_tag="PLB85_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72673.1"
                     /translation="MSMAGNRLHINAKDAAGFGLSYVAEILFDNTANTSLAGSEAGLI
                     HLYSKTPIFCYDIPITAASMSYAHARIHTIDTQGQKAVTTTLHSAVVFDSNVISDNFV
                     NYLAGRIANLLETAGSEAIVYKGN"
     gene            3306..3614
                     /locus_tag="PLB85_04755"
     CDS             3306..3614
                     /locus_tag="PLB85_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72674.1"
                     /translation="MLIVGSVTVPGRLLPTRSPHANVLTPNQLRQAIATLKPYASKAA
                     HEAKKKLRASIMSHVSRHGQDIDWLHQNMISWGFGDSLRACSHNQTLQIYSLVKKALP
                     "
BASE COUNT          624 a         1069 c          976 g         1053 t
ORIGIN      
        1 ctgggattcg aattccgcca gctcctggta tagctcgccc agccgttctg ccagcagcat
       61 ttctttctgg tccaggctca gtttcagctc gttcacgtcc tcgatgatcc gcgctttttt
      121 ccactcgtaa tagttcgcgt cgaggaattt cacttcctca taatatcgcg ccgtgtcgga
      181 ttccctgtta accgccagtt gtgctgcctg agcccgtctc aggtaaatgt tcaaaagctc
      241 cttctcggcc tctgtcagtt ctccatctcc ctcaacgatt tcctttagtt tcccatagta
      301 atcccagctt gccgcggcga tttcgccgta agttgccagc cccaggtcac gcagttcggc
      361 caggtggctc atttctgaat cgtacttttc ttcaaaggcc gcaatggccc gttgatccca
      421 ctcttctcgt tctcgattca gttgctcgat ttgctcatct ttccaggctg cgggaacatt
      481 cggatcctct gcctctttat tgatctgttc cagcttccag tcataatatc ccacggcaaa
      541 gaacttgagc ttttcatagt gtgcggcctc ttcttcctct cgctcttcct gatacttctt
      601 ttgaatttcg cccacctcac ggtctcgctt ttccgacagg tctgctgtag ccgccttgtc
      661 ttttgcgacc agtttgagct tctcggtgta ttcccgcttc agaagctcta tctctgtctg
      721 ttcctcattg aatttgtttt cccggaggat gcggtattct gttatctcgg agatcacttg
      781 gtcgagttcg gttttcttgg aagcggcggt tttccctgag cctttgcctt tccccttgtt
      841 cttgttgctt tcttcgtttg atggtgccaa agatgtgtct tcacccagcc tggaaaccag
      901 ttgatcgatt tccttgtccg tctttttgat ctcagcctta agcttcttga tgtctttctc
      961 taatttttct tcctgtttcg cccatttcga cgaaactaaa attaaacgtg gcatatttgg
     1021 actgctcgaa agcgggtctg gagctgcctg tctataagcc tcgcgctttt ttgttcctgc
     1081 ctcgaactcc gcctgcaggg tttttagctg attttctttc tcattgagct ttttgtagag
     1141 ctttacgtaa tcgtccgtct tgtcgtcgat gatggcctgt ttgatcttgg cgttggtcca
     1201 ttcctgaagc ttgcttcttg cggagttcag ggctgccgcg atgtcttccc acgccccttt
     1261 ttcgagatcg atgttcccga ggtagttcgg atacttggtc atcaggtcat ttatggtttt
     1321 ccgatacttt tcgttttctt ctttgctgcg gttctgcctg aagtgcagtt ctttatatac
     1381 caggacaagc ttttcgaatt ccacccgctg gttcacgagc ccttctttta ccgcatcgag
     1441 gccgcttttg gtccccgaaa gcttgtctgc gagcttggca agtgcgtttg cggctgctcc
     1501 gatgatgggg gttatcttgt ttccgatggc tgcatagagg ctctccatgg cgtcctgcat
     1561 gttggcgacg gctccggtgt agctttcggc catgcggtcg gaggagccag cgattccggc
     1621 agcggcatcc gagaaggtct ccagcatggc ttcgcggaac tggggcagcg tgagcttggt
     1681 gaggtcttca acgcccttga agcttttgat gagttcgagg acgccgcgtt cgcggaggac
     1741 gtctgcggct ccgacgcccc cggcgaaggc cctgcccacc gcctgggcgg cctcgacgat
     1801 atccatgccc atgtaggcgg cgagatcggc gactgaatcg aggacgtttt cggcatcgag
     1861 tccaaaggct ttgagggtgg ctccggcctc gaccacccct ttgagcgtgg cgggggtctg
     1921 ggcggccacc tggcggaatt tctcaaacgc cgctgcggcc ttgtcggcat cctggtaaag
     1981 ggattcgagg cggagctgga gctgctcgaa ttccgctgcg gattccacga acttgcgcac
     2041 ggtggcgttg taggctccaa cgaccgcgcc gatggcggag gagatgccgt tcatgaccag
     2101 ccccagttcc ttggctctgc gtttgaaatc gtcgagggcg ctgatctgtg ttttgaggct
     2161 ggttccgatg tccgcgaagt cggcatcggc gtggagcttg tattttttgg agagcttttc
     2221 gataatgtct tccagctttt cctttccgga aagctcgatg ctgatcacca gtttggaatt
     2281 actcatttgc tttttctccg ttttcgctta tgctggtttt agaggtaaac ccaatgctta
     2341 taatagccgc gctcatcctt ctgctgccgc ttctggcggt gctgggcttt ctgcttgtgc
     2401 tctttttcaa gggcaagcct ggcgagactg accccaccaa gctttaggct gtttcgccgc
     2461 tgaaggggca aaagcgggcg gaggcttcct ccgcataaat ctccgcaagc tggagggctt
     2521 catccagttc cacccgccgg ggctccgctc cgatgcggta gaggatgtat cttagtttca
     2581 tgaagatatt tgtcttaggg ctgtctggag cgcattcatc agatgttggg attctgcctg
     2641 caatccgatt aaactgaaaa aaaaagccag gatgacggcc atcgtttcct cacgggagca
     2701 ctggtcccag ggcttttttt ccttggaagc gggctgcacg caggtttcgc tgcgggtgat
     2761 gagggcgcag acttccggga tgccgccggt ttgcaggagt gcggcaaaga gctccggata
     2821 gccgtagccc ctgatcttct ccaggtctcc ggtgagcata ccctcgatgc ccgcttcctg
     2881 gaagatggga tagagtttgg agagggtggt tacgtcggta tgtatcttca tcttagttcc
     2941 ccttgtaaac gattgcctca ctgccggccg tctcaagcag gttggcgatc ctgccggcca
     3001 ggtagttcac aaaattgtcg ctgatcacgt tgctgtcaaa gaccaccgcg ctgtgcagtg
     3061 ttgtggtcac ggctttctgt ccttgcgtgt cgatcgtgtg gattctcgcg tgggcgtagc
     3121 tcatgctggc cgcggtgatg ggtatgtcat agcaaaaaat gggcgtcttg ctgtagaggt
     3181 ggatcagccc cgcctcactg ccggccaggc tggtgttcgc tgtgttgtcg aaaaggattt
     3241 ccgccacgta gctcagcccg aatccggccg cgtccttggc gttgatgtgc agccggttgc
     3301 cggccatgct cattgtcggc tcggtcaccg ttccgggcag gctgctgccc accaggtcgc
     3361 cccatgccaa cgtgctcacc cccaaccaac tccgccaggc catcgccacc ctcaagccct
     3421 atgcctcaaa agccgcccac gaagcaaaaa agaagctccg cgcatccatc atgagccacg
     3481 tctcacgcca cgggcaggat atcgattggc tccaccaaaa catgatctcc tggggcttcg
     3541 gcgattccct gcgcgcctgc tcccacaacc agactctgca gatctactcc ctcgtcaaaa
     3601 aggctctccc atgaaattct caactctcaa ctatcaactc ttaactctcc cacgtcattc
     3661 cgggctcgac ccggaatcca gcccctccct ggcgtccagc gaacgtagtg agcatggacg
     3721 gc
//
LOCUS       DAOOWR010000163         3722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24813_length_3722_cov_2.738478, whole genome shotgun sequence.
ACCESSION   DAOOWR010000163 DAOOWR010000000
VERSION     DAOOWR010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24813_length_3722_cov_2.738478"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLB85_04760"
     CDS             complement(<1..213)
                     /locus_tag="PLB85_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72675.1"
                     /translation="MMVRKILIISLALLLCLVLSACKHKEKESVPETMPGTEFRHDAT
                     LTVSAPNGSVKARFEVEIAATPEATMQ"
     gene            complement(210..>446)
                     /locus_tag="PLB85_04765"
     CDS             complement(210..>446)
                     /locus_tag="PLB85_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72676.1"
                     /translation="PSTRIEFSLPKDQEAWLGIYNTRGQLMKTLLNGQTAKGSHQTVW
                     NGLDDNGLPVASGIYLCKLVSGGKSQVRKMILMK"
     assembly_gap    447..456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<457..3138)
                     /locus_tag="PLB85_04770"
     CDS             complement(<457..3138)
                     /locus_tag="PLB85_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU72677.1"
                     /translation="MKKHSLILFLVLQSALAIAFPARINSWNVDRDLKTLNAYNINVD
                     NVNRATGTIVVYLKDYSEMALLLDEGFSAEKLPDLARENAARLHQNSSKDAPKDDYYT
                     ITQYNQFMLDTAAQYPNICSLSQIGTSVQGRPLYFMKITDNPSLEEAEPEFKFISSIH
                     GDEVVGYDMCIRLIQLLTSEYGNNTRISNLVNNTEIWICPMLNPDGFVLGQRFNATGI
                     DLNRNFPMPFGGNQHPDGNPWAHETIAVMDFGNLHNFVLSANYHGGALVANYPWDYTF
                     ALAPDNALLIEAALTYARHNLPMYNSTEFNQGITNGAEWYVITGSMQDWNYGFTDCMD
                     ITLEISNNKWPPASQLPTFWAQNQESMLSFMEFAQRGIHGLVTSQSGSPLSATITVQG
                     NAKVMHTDPAVGDFHRLLLPGTYTVTASAPGYLPQTVDITVPVSGSVEYNFVLDEAQA
                     VDLIGQLRDIDGFGIPGVSVSIDTVPASNATADALGCFVFSGIPEGQYHITFRSGNQL
                     VHEQDILLTMFNNRLVFVQIEPQQSFYDPCESITNWTANGPWAAVTHMGESVITDSPS
                     GNYANDTLRILRITNPISLQNIIEPTLSFKAVYDLENGYDFVLVQASTTTSNWTELGR
                     ITGTQADWQDFSYSLQQFSGQNVYIRFALDSDWSVNGDGIYLDDITVAGIGADQIVFG
                     DVDSDRLISVKDAQAVIDYTVGFDPLPDIDPLPWSAERVTAADVDLSLVLDSEDAWLI
                     GQYLFDPEFRFQAQSGEEAVLPSVSLNAEHIDDPSFIYYLVSGTPAGEFYCIDWQLFP
                     QDGVTYSEITPSGDIFDGISAYNPHQLKYAHAQLGGSGNLSFALAYNTSLPGINLAYS
                     VNGQSDVLNLPSSVANDDLVESPAVFALQQNYPNPFNP"
     gene            complement(3199..3297)
                     /gene="ffs"
                     /locus_tag="PLB85_04775"
     ncRNA           complement(3199..3297)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLB85_04775"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3396..>3722
                     /locus_tag="PLB85_04780"
     CDS             3396..>3722
                     /locus_tag="PLB85_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72678.1"
                     /translation="MKTYTSLIILLLLTAYLSAGAWQMATGFGSPAKESAWDLAVQPN
                     NFNPQYFWVCGEFTDSLSIGGVNYLSNGLSDCFVAKIAVDGEPHWVRTFGSNDGDVAL
                     SVAADMD"
BASE COUNT          870 a          945 c          997 g          900 t
ORIGIN      
        1 ctgcatggtg gcttccggag tggcggctat ctccacttca aacctggcct tgacgctgcc
       61 gttcggggct gaaaccgtca gggttgcgtc atggcggaat tctgtgccgg gcatggtttc
      121 gggcacggat tctttctcct tgtgcttgca ggcgctcaga accaggcaaa gaagcaaagc
      181 aagggatata ataaggatct tgcgtaccat catttcatca ggatcatttt acggacctgg
      241 cttttgccgc cacttacaag tttgcagagg taaatgccgc tagccacagg caggccattg
      301 tcgtccagtc cattccagac tgtttgatgg ctgcccttgg ccgtctgtcc attaagcaat
      361 gttttcatta actgcccgcg ggtgttgtag atacccagcc aggcctcttg gtctttaggc
      421 agggaaaatt cgatccgggt gctcggnnnn nnnnnncgga ttgaagggat tgggataatt
      481 ctgctgaagg gcaaaaacag ccggagactc taccaagtca tcattcgcca cgctggatgg
      541 caaattcagc acatcggact ggccgttgac gctgtaagca aggtttattc caggcaggga
      601 agtgttgtaa gcaagagcga aactcaggtt tccgctgcct ccaagctgcg catgggcgta
      661 tttcaactga tgggggttgt acgctgaaat accgtcgaaa atgtccccgc ttggggtgat
      721 ctccgaatac gtaacgccat cctggggaaa cagttgccag tctatgcaat aaaactctcc
      781 tgccggagtc ccactcacca aataataaat gaaacttggg tcgtcgatat gctctgcatt
      841 cagcgaaacg gacggcagga cagcctcctc cccactctgg gcttgaaagc ggaactctgg
      901 gtcaaacaaa tactgaccta tcaaccaggc gtcctcggaa tccaaaacca ggctcaggtc
      961 aacatccgcg gcggttaccc gttccgctga ccaaggcagg gggtcgatat cgggcagggg
     1021 atcaaaacca acagtgtaat cgatcacggc ctgggcgtcc ttcacactga tcagcctgtc
     1081 gctgtccacg tcgccaaaaa caatttggtc tgccccaatg ccagccacgg ttatgtcatc
     1141 gaggtagatg ccatcaccgt tcacgctcca gtcagaatcc aaagcaaagc ggatgtagac
     1201 attctgacct gaaaattgct gcagggaata gctgaaatcc tgccagtcag cctgggtgcc
     1261 ggtaatccgg cccagctccg tccagttgga ggtagtggtc gaagcctgca ccagcacgaa
     1321 atcgtagccg ttttcaagat cgtaaacagc cttgaagcta agcgttggtt caattatatt
     1381 ttgcaaggag ataggattag tgatgcgaag tattcttagc gtatcattgg cgtaattacc
     1441 agagggtgaa tctgttatca cgctctcacc catgtgggtc accgctgccc aggggccgtt
     1501 ggcggtccag ttagtgatgc tttcgcaggg gtcgtaaaag ctttgctgcg gttcgatttg
     1561 cacaaaaaca agacggttgt taaacatcgt gagcaggatg tcctgttcgt gcaccaactg
     1621 gtttccagac ctaaaagtta tatgatactg cccctcgggg atgccgctga aaacgaagca
     1681 gcccagggca tcggcggttg cgttgctggc tggcacagta tcaatgctca cactcactcc
     1741 gggtattcca aagccgtcta tgtccctaag ctgacctatg aggtcaactg cctgggcttc
     1801 gtcaagcaca aagttgtatt caacagaacc tgagacaggc acagtgatat ccacagtttg
     1861 aggcaggtag cccggagcgg aggccgtaac agtataagtc ccaggcaata acaagcggtg
     1921 aaagtctccc acagcgggat cggtgtgcat caccttggcg ttgccctgaa ccgtgattgt
     1981 ggcgctcagc gggcttccag actgcgaagt aacaaggccg tgaatgccgc gttgggcgaa
     2041 ctccataaag ctcagcatcg attcctgatt ttgcgcccag aaagttggca gttgcgatgc
     2101 tggcggccat ttgttgttgc taatctccaa ggttatgtcc atgcagtcgg tgaagccata
     2161 gttccaatcc tgcatggagc cggtgatgac ataccattca gctccgttag tgatgccctg
     2221 gttaaattcc gtgctgttgt acatgggcag gttgtggcgg gcgtaggtaa gcgcggcttc
     2281 gatcagcaga gcgttgtcgg gggcaagtgc gaaggtgtaa tcccaagggt agttcgccac
     2341 cagcgctccg ccgtgataat ttgctgaaag cacgaagttg tgcaggttgc caaagtccat
     2401 cacggctatg gtttcatgcg cccaggggtt gccgtcggga tgctggttcc ctccgaaagg
     2461 catgggaaaa ttgcggttaa ggtcgatccc cgttgcgttg aagcgttgcc ccagcacgaa
     2521 tccgtctgga ttcagcatcg ggcaaatcca gatctcggtg ttgttcacta gatttgagat
     2581 tctggtatta ttgccgtatt cggaggtgag cagttggatc aggcggatgc acatatcgta
     2641 gcccacaact tcatcgccgt ggatggaaga gatgaatttg aactctggct cagcttcctc
     2701 gagggaggga ttgtccgtaa tcttcataaa gtaaagcggc ctgccctgca cagaggttcc
     2761 tatctggctc agggagcaga tgttgggata ctgcgcggcg gtatccagca tgaactgatt
     2821 gtattgggtg atggtgtaat aatcatcttt gggtgcgtct ttggagctgt tctgatgcag
     2881 gcgggcggcg ttttcccgcg ccagatctgg aagtttctca gccgagaaac cctcatccaa
     2941 caacagtgcc atttcgctgt agtcctttaa atagacaacg atggtcccgg tggcacggtt
     3001 cacattgtcc acgttgatat tatacgcgtt gagggtcttt aggtccctgt caacattcca
     3061 gctattgatc cgggctggaa aggcaatggc cagcgcgctc tgcaacacca gaaagaggat
     3121 gagtgagtgt tttttcatct ataaactcct tgattaacca aacaataatg caaaaatcaa
     3181 gccataacca gccttgtaaa aagccaggct gacagcggca cacgactcgc gttgcttaat
     3241 gctgcttcat tcctgatctg acatggttca caagctgccg ttgcactgcg cctggccaag
     3301 ccacaatttc cctcccgcct cttttgtcaa ctgatttcca actttttcct tgtcaaaata
     3361 accatgcctt aaaagtggca tcaggagcaa taacaatgaa aacatatacc agtctgataa
     3421 tcttactgct gcttacagca tatctgagtg ccggcgcttg gcaaatggcc accggattcg
     3481 gctcgccagc caaagaatcc gcctgggacc ttgctgttca gcccaacaac ttcaaccccc
     3541 agtatttttg ggtatgcggg gaattcacag attctctaag catcggcggc gtgaattacc
     3601 tttccaatgg cctttcggac tgctttgtgg caaagattgc cgttgacggc gaaccgcatt
     3661 gggtgaggac tttcggctcc aacgacgggg atgtggccct ctcagtcgcg gcagacatgg
     3721 ac
//
LOCUS       DAOOWR010000164         3705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_24945_length_3705_cov_3.735616, whole genome shotgun sequence.
ACCESSION   DAOOWR010000164 DAOOWR010000000
VERSION     DAOOWR010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24945_length_3705_cov_3.735616"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLB85_04785"
     CDS             <1..310
                     /locus_tag="PLB85_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU72679.1"
                     /translation="RHIQRTSLLLFLIDIGTPDLVDTFRTLRSELYFYDPLMSKKPSL
                     VAFSKLDTIPAEERDELVSTVNSQFQKEFGVETIPISSVSHINLDELKYRLFKLLQNL
                     "
     gene            307..1398
                     /gene="dprA"
                     /locus_tag="PLB85_04790"
     CDS             307..1398
                     /gene="dprA"
                     /locus_tag="PLB85_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU72680.1"
                     /translation="MTEQLQAWLCLKSAPGLKHRAALDLLSRYPDPLAFVGRKDHPLY
                     ADNRLDPALLEHLRNATPHPRLQQIAKLCEHYSIEAVFYGTDNYPPGLANILAPPLIL
                     WHRGDLAPALERVCLAVVGTRKPTAYGRGSCAKLLAPVCKQGTTIISGLAIGIDTVAH
                     YAALENGSQTVSVLASGLETIYPPSNRDLANQIITRGALVSEYEPSTKLDPWNFVARN
                     RIISALARNVFIVEGPLDSGAMITAKHAIEQNRDLMALPGEINHPNAQGPNYLIKNGA
                     QCVTSPEDILSALGLDVTDNQQLEILPELSADEQKLLDLFTSEQRELSFDDLLIRTGH
                     KFGKLSTLLLNLELKGCISKTGGNSFILG"
     gene            2109..>3705
                     /locus_tag="PLB85_04795"
     CDS             2109..>3705
                     /locus_tag="PLB85_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72681.1"
                     /translation="MKKVLFFTVILAAFAFSMSAQYTEVYTQPCFSDVGNLYTSALDT
                     ADNINYEIADNFVNVTDPIKKVEFYGACGSFAGDWYPGTPNAQEPFIIRFYQQVTGWT
                     VPPEPLTAPETGTYTVKLYDSYGDGWNDGKLDVLVNGVVVLDDITLASGAGPAAYTFS
                     ANAGDIISTIFTAGSWANENWYEILDPNNVVIATDGVGTNPPTGIDVPQPPQPLIAPA
                     TGTYTVNLYDDYGDGWDTSLLSVYVNGAIVLENITLATGAGPEAYTFTANTGDEVCTV
                     FKAGAWPGENWYEILDPDSVVIATDGPNPQGIGFIPEEILYEPTWGTPDYEFNVTCTT
                     SYYMDAWDTWEVYKFEAVLPQEVVLENGWFSAQIDVDSGASNLFLFAPGTGLDQFSIQ
                     HVYAKSRLDKEIQKKLMMVSSGNYRDPIANDIAFTLYKEDVVAPEITFILDPPTPGVP
                     VYLNGELLGYTNDNGVFTCPLPQGEGPFVYTFGAPEDGWDSWQFSPQDNYELIITQDQ
                     NPTPVVLSSFTATVTAVNDVKLSW"
BASE COUNT          874 a         1071 c          883 g          877 t
ORIGIN      
        1 gcgccacatt cagcgcacca gcttgttact ctttctcatc gacattggca cacctgacct
       61 tgttgatacc tttcgcaccc tgcgttccga gctgtatttc tatgacccct taatgagcaa
      121 aaaacccagt ttggtggcat ttagcaagct tgacaccatt cccgccgaag agcgcgatga
      181 acttgtttct accgttaact ctcagttcca aaaagagttc ggggtggaga ccatccccat
      241 ctcctcagtg agccatatca acctggatga gctgaaatac cggctcttca aattgctgca
      301 aaacctatga ccgaacaact tcaggcctgg ctctgcctca aatctgcgcc cggattgaaa
      361 cacagggccg cgctggacct tctgtccaga taccccgatc cgctggcttt tgtggggaga
      421 aaagaccatc ccctctatgc cgacaatcgc ctcgatccag ctctgctgga gcatttgcgg
      481 aacgcaacac cacatccccg gctgcagcaa atcgccaagc tgtgtgagca ttacagcatc
      541 gaagctgtgt tttatggtac cgacaactat ccccctggcc tggccaacat ccttgccccg
      601 ccactgattc tctggcatcg gggtgatttg gccccggcgc tggagcgggt ttgcctggct
      661 gtggttggca cccgcaaacc cacagcttac ggacggggaa gctgcgccaa gctgctggct
      721 cccgtttgca aacaaggcac caccatcatt agcggactcg ccatcggcat cgatacagtg
      781 gctcactatg ccgccctcga aaacggttca cagaccgtct ccgtgctcgc cagcggcctc
      841 gagaccatct atcctcccag caaccgcgac ctggccaatc agataatcac ccgcggcgcc
      901 ctggtttccg aatacgagcc aagcaccaaa ctcgacccct ggaactttgt cgcgcgcaac
      961 agaatcatct cagccctcgc gcggaatgtc ttcatcgtgg aaggccctct ggacagcggc
     1021 gccatgatca ccgccaagca tgccatcgag caaaaccgcg atctgatggc cctccctggc
     1081 gaaatcaacc accccaacgc gcaggggccg aattacctga tcaaaaacgg cgcccaatgc
     1141 gtcacctccc cggaggacat cctttccgcc ctgggcctgg atgtgactga taatcagcag
     1201 ttggagatat tgcctgaact cagcgccgac gagcaaaagc tcttggacct cttcacaagc
     1261 gaacaacgcg agttgagttt cgacgatctg ttgatccgca ccggccacaa gttcggcaaa
     1321 ctttccaccc tccttctaaa ccttgaacta aagggctgta tcagtaaaac cggaggcaat
     1381 tccttcatcc tgggctgatg accgtgccca cataaagcac aactttaggt cttcccataa
     1441 aatcctcttt ttcggcgttt cccgttctca tttttcttgt cccacccaag ttgtcaactc
     1501 cttcctccat ttcaaacaca tctcccctaa ctacctgctc tcttacaaat cgtgtggttt
     1561 ggccccggag agcagcatag aatagcggag gtgtgaagtg gcctgggaga agcaatgcta
     1621 tcaggccgtt aaggccctgt cccggttcac ggacgcgcct cccgcatgat acccgcattt
     1681 gatgcgagaa tcgtgcggga ggcatgggag tgcgatggac tcgagcgttc agggcctgac
     1741 tgacaggata ctgctgtgac taagcatttg tggctttcca ttgaatcagg ccctctttca
     1801 aaccgggtcg gtaatccgtt tggaagggag atcagtaata tgtgatggga aggtaaattc
     1861 gaaagcttga gaaagttctt gacaaatttt cagactggct tgatgaggca cataattgca
     1921 tttatgggtg ttgcgggtaa aaatatgaaa tctcgtcagt cttgtttgaa ttttttttca
     1981 ggcaaagctg aaaacataga atgtgattgg gaaggcttgg cgaatccgct ttaagtcttt
     2041 ggttttaacg aaaaaagatt gccagggctc accgatgagt cccggcgact acaaaggaga
     2101 attcacaaat gaagaaagtt ctttttttca ccgtgatatt ggcggcattt gccttcagca
     2161 tgtcggctca atacacggaa gtttacaccc aaccctgttt cagcgatgtc ggaaacttgt
     2221 acaccagtgc cctggacact gccgataata tcaactatga gatcgccgac aacttcgtta
     2281 acgttaccga tcccatcaag aaagtcgagt tttatggcgc ttgcggatcc tttgccggag
     2341 actggtatcc cggcaccccc aacgcgcaag agcccttcat catccggttc tatcaacagg
     2401 taactggctg gactgttccg cccgaacccc ttactgcccc tgaaaccggc acctacaccg
     2461 tgaaacttta cgacagctat ggtgatggct ggaacgatgg taaactggat gtcctggtga
     2521 acggagtcgt tgttttggac gacatcaccc ttgcctccgg cgctggtcct gcagcttaca
     2581 ctttcagcgc caatgccggc gacattatct ccactatctt cactgccggc agctgggcca
     2641 atgagaactg gtatgagatt ctcgatccga acaatgttgt tatcgccacg gatggcgtag
     2701 gcacgaaccc acccaccggt attgatgttc cccagccgcc ccaacccctg atcgctcctg
     2761 caaccggcac ctataccgtg aacctttacg acgactatgg tgatggctgg gacaccagcc
     2821 tactgagtgt gtatgtgaat ggagccatcg ttctggagaa catcacactt gccactggcg
     2881 ctggtcctga agcttacact ttcaccgcca ataccggcga cgaggtctgc accgttttca
     2941 aggctggcgc ttggcctggt gaaaactggt atgagattct cgatccggac agtgttgtca
     3001 tcgccaccga tggccccaat cctcagggaa tcggattcat cccagaggag atcctgtatg
     3061 agcctacctg gggaaccccg gattatgagt tcaacgtgac ttgcaccacc tcctattata
     3121 tggacgcatg ggatacatgg gaggtttaca agttcgaagc tgtccttccc caagaagtgg
     3181 tactggaaaa tggctggttc tcagcccaga tcgacgttga cagcggagcc tccaatctgt
     3241 tcctctttgc ccccggtaca ggccttgacc agttctccat ccagcacgtt tacgcgaaga
     3301 gccgtctcga taaagaaatc caaaagaagc tgatgatggt ttcttccggc aactaccgtg
     3361 acccaatagc gaacgacata gccttcaccc tctataaaga ggacgttgtt gctccagaaa
     3421 tcaccttcat tcttgatcct cccacccctg gcgtgccggt ttacttgaat ggtgaactcc
     3481 ttggttatac caatgacaat ggcgtattta catgccctct ccctcaaggt gaaggaccct
     3541 ttgtttatac cttcggagca cctgaagatg gatgggatag ttggcaattc agtcctcagg
     3601 ataactatga actgataatt actcaggacc agaatcctac ccccgtggta ttgagcagct
     3661 tcaccgccac cgtgaccgcg gtcaacgacg ttaaactgtc gtggg
//
LOCUS       DAOOWR010000165         3689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25098_length_3689_cov_3.729774, whole genome shotgun sequence.
ACCESSION   DAOOWR010000165 DAOOWR010000000
VERSION     DAOOWR010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25098_length_3689_cov_3.729774"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLB85_04800"
     CDS             <1..280
                     /locus_tag="PLB85_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOU72682.1"
                     /translation="GYGVANAFRRTGLFPPTLLQMIGTGEETGDMDKLLGKAAQFYEK
                     LVDAVIERLTSLIEPFLIIIMAAVVGGIIVVVYLPIFSLGEAMTQGIK"
     gene            complement(811..2910)
                     /locus_tag="PLB85_04805"
     CDS             complement(811..2910)
                     /locus_tag="PLB85_04805"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOU72683.1"
                     /translation="MTQSSNPLLRPKSSHRLQAIPFDEIRTEHFMPAIEETLKTAKAE
                     IEALKNNPAAPDFENTILALDLYGEQLDHVAGIYFNLLSAESDSEFKALAQRISPLLA
                     EFNSSISTDEAIFARVKKVWEDEVEGKPKPELPKDMNDRDALKQAERYRLIDRAYQGF
                     IRGGALLSTEDKQKLTAIAMESSKLSPQFSDNVLGATNAWELHITDPADVEGMPQGVL
                     AGAAHLAKSKGKDGGWLFNLQPSSMVPLLTYCKNRELRRQSQTAYASRAFNDDFDNQE
                     LIKRALDLRQQRADLLGYPTHADYVLSDRMAESVQTATEFLEKIYSVAYPAAKQEYQE
                     VVDYAKQTDGISDFKPWDMGYYSNKLKEARYAYDPEELRPWFKVENVLTGLFTVAKHI
                     YGIEVKQVTDVPTWHKDVSTWEVYDRAGDFLGLMYMDLFPRETKRGGAWQTSFQRQGL
                     HSDGLRRPHVAIVASLTPSTEDQPSLLRLDEARTVFHEFGHALHSLLADGYYKGLSGT
                     SVLWDFVELPSQIMENWLLEEEALNLFARHYETGEPLPSELLKKVIDAKNFQAGLANI
                     TQLRYAMLDLAWHTTHPSKITDVDAFEKQATARFQIAPPIEGANISCAFAHIFAGGYS
                     AGYYSYKWAEALEADAWSLFDEKGIFNPEVSEAFRKYILARGNAFHPMDLFVAFRGRK
                     PDPDALLKRDGLIPTPA"
     gene            3273..>3689
                     /locus_tag="PLB85_04810"
     CDS             3273..>3689
                     /locus_tag="PLB85_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72684.1"
                     /translation="MLKKLLFSLTLILALGFVWGQGLETFDNMPATGSAYGTGTFTGQ
                     DGSTWNYVQCRSDVDVTGKSCMLGRNRTPQSNVYSGTISGGIGTLSFNYMQAYSTNVN
                     LNVLVNGNVVGNVTSSGQQNVILNSGTITVNVPGDFV"
BASE COUNT          735 a         1004 c         1030 g          920 t
ORIGIN      
        1 aggctatggc gtggccaacg cctttcgccg caccggattg tttccgccca ccctcctgca
       61 gatgatcggc accggagagg aaactggcga catggacaag ctgctgggca aagctgccca
      121 gttctacgaa aaactggtcg atgccgtgat cgagcgcctc acttcgctca tcgagccctt
      181 ccttatcatt atcatggccg ctgtggttgg cggcatcatc gtggtggttt acctgcccat
      241 cttcagcctc ggcgaagcga tgacccaagg catcaaataa tccattcaac cataaagtct
      301 tgcaaggccg gttcaccccg gccttttttg attgccggcc tcccccttgc ctcctcgccc
      361 ggccaattag tgttcctgcg cagcctccag ctgttttggc tccggagcca ggccgttccg
      421 cttctcactt cgaccgtcac ggtcactggc gagcctggac atgtattcca tcaaatcgat
      481 agaccgtctc tccagcaggt aagaaagaga gccagaattc ctcaaccact tcatttgaac
      541 gtcataactg cttagccacc gcggaatcct gccagtcacg ccctgagcgc tcgtgtccat
      601 ccctctttca tgcctcccgt atgatactcg catcaaatgc gggcatcagg cgggaggcat
      661 atccgtgacc aaggacaggg cgttaacggc catactatgt cttttcgctt gttcaaggca
      721 ccaggatgtc gcatccataa tatatggaca taagccaaaa aaaacgccgg cgatagcacc
      781 ggcgttccat tactctctat tttcaggggt tcaggctggt gtggggatca gcccgtcgcg
      841 cttgagcagg gcgtcggggt ccggtttgcg tccccggaag gccacaaaga ggtccatggg
      901 gtggaaggcg tttccgcgag ccaggatgta tttgcggaag gcttcactca cttcggggtt
      961 gaatatgccc ttttcatcga agaggctcca ggcgtcggct tccagcgctt cggcccattt
     1021 ataggaatag tagccggcgg aatagcctcc ggcgaagatg tgggcaaagg cgcaggagat
     1081 gtttgctccc tcgatggggg gtgcgatctg gaagcgcgcg gtggcttgct tttcaaaggc
     1141 gtccacgtcc gtgatcttgc tgggatgagt ggtgtgccag gccaggtcga gcatggcgta
     1201 gcgcagttgg gtgatgttgg ccagtcccgc ctggaagttt ttggcatcga tcactttctt
     1261 cagtagttca ctgggaagcg gctcaccggt ttcatagtgt ctggcaaaga ggttcagcgc
     1321 ctcctcctcc aggagccagt tttccatgat ttggctgggc agttccacaa aatcccaaag
     1381 cacgctggtg ccggagagtc ccttgtaata cccgtctgcc agtagggaat gcagcgcgtg
     1441 gccgaattcg tgaaagacgg tgcgggcttc gtccagacgc agcagcgagg gctggtcctc
     1501 cgtggagggg gtcagcgaag ccacgatcgc cacatgcggg cggcgcaggc cgtcggaatg
     1561 cagtccctgt ctctggaagg aggtctgcca ggccccgccg cgtttggttt cccgagggaa
     1621 gaggtccatg tacatcaggc ccaaaaaatc cccggcccgg tcgtaaactt cccaggtgct
     1681 cacatccttg tgccaggtgg gaacatctgt aacctgcttc acttcgatgc cgtagatgtg
     1741 tttcgccacg gtgaagaggc cggtcagcac gttttccacc ttgaaccagg ggcgcagttc
     1801 ctccggatcg taggcgtagc gtgcttcctt gagtttgttg gaatagtagc ccatgtccca
     1861 gggcttgaaa tcgctgatgc cgtctgtctg tttggcgtag tcaaccactt cctggtattc
     1921 ctgtttggcg gcgggatagg caacgctgta aatcttttcc aaaaactccg tggcggtttg
     1981 cacggattcc gccatgcggt ccgaaagcac gtagtcagcg tgggtggggt agcccagcag
     2041 gtcagcgcgt tgctggcgca ggtccagggc gcgtttgatc agttcctggt tgtcaaaatc
     2101 gtcattgaag gcgcgcgagg cataggctgt ctggctttgg cggcgcagtt cgcggttttt
     2161 gcagtaggtg agcagcggta ccatgctgga tggctgcagg ttgaagagcc agccgccgtc
     2221 ctttcccttg cttttggcca gatgagccgc cccggccagc acaccctggg gcatgccttc
     2281 cacatccgcg ggatcagtga tgtgcagttc ccaggcattg gtggcaccta atacgttgtc
     2341 ggaaaactgg ggcgagagct tggaagattc catcgcgatg gcggttagct tttgcttgtc
     2401 ttccgtgctc agcaaggcgc caccccggat aaagccttga taggccctgt cgatcaggcg
     2461 gtagcgttct gcttgcttca gcgcatcgcg gtcgttcatg tctttcggca gttcaggttt
     2521 cggtttgccc tcaacctcgt cttcccaaac ctttttcacc cgggcgaaaa tcgcctcgtc
     2581 ggtggaaatg ctgctgttaa attctgccag cagcggtgat atccgctgcg ccagggcctt
     2641 gaactcggag tcggattccg cggaaagcag gttgaaatag attccggcca catggtccag
     2701 ttgttcgccg tacaggtcca aagccaggat ggtgttctca aaatcaggcg cggcgggatt
     2761 gttcttcagc gcctcgatct ccgccttcgc ggttttaagg gtttcctcta tcgccggcat
     2821 gaaatgctcg gtccggattt cgtcgaaggg tatggcctgc aggcggtggc tgctttttgg
     2881 cctcaacagg gggttgctgg attgtgtcat ctggatgtcc ttttcattat tctcatttga
     2941 aaacacgaaa atccaaagcc cccgctctgt caatccgttt ttgccatttt ccgccctgtt
     3001 ccgtcgcttg cgggattccg gcaagatcgc tgatttttct tccccgaccc cggaaaaaaa
     3061 cattgacgga tatacacgct ttttagattg taactttagg gggatatcgc ccccgctttg
     3121 ttattgcagt ccataggttt gtgttatgtt atggcttggc ttgggtcttc tgacccgcca
     3181 aaacaccccc tctttacatc gaaatgtatc acgctgccgg tcagagaccc cggcaccata
     3241 cggtttttaa tgacttaagc aaggagatga acatgcttaa aaaacttcta ttttcactaa
     3301 ccctgattct cgcattgggc tttgtctggg gtcagggttt ggaaaccttc gataacatgc
     3361 ctgcaactgg ttctgcatat ggcacaggca ccttcacagg acaggatggg tcaacctgga
     3421 actatgtcca gtgccgcagc gatgttgacg taacgggaaa atcttgcatg cttggaagaa
     3481 acagaacacc gcaatccaat gtttattccg gaactatttc tggtgggatt gggacgctgt
     3541 ctttcaacta tatgcaagcc tattcaacta atgttaacct gaatgtgctg gttaatggca
     3601 atgtagtcgg caacgtgacc tcttccggac agcaaaatgt catactgaac tcaggaacta
     3661 taactgtcaa tgtgcccgga gatttcgtt
//
LOCUS       DAOOWR010000166         3659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25365_length_3659_cov_3.619312, whole genome shotgun sequence.
ACCESSION   DAOOWR010000166 DAOOWR010000000
VERSION     DAOOWR010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25365_length_3659_cov_3.619312"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLB85_04815"
     CDS             complement(<1..588)
                     /locus_tag="PLB85_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72685.1"
                     /translation="MKTRLFFVFSVAVLVFLFVLSGCHKPTDPNKVSKPVFDPPGGTY
                     DSAVEVVITTSTLGTTILYTTDGSDPDSNTPVYAGPILVSETITLKAKAYRTGMQDSA
                     IATASYTIELPPPPENFVLVTGGTFTMGDTRGQGDPDDELPTHTVTLNSFYIGKYEVT
                     QAEYSQYMQPGSGWTSSFGLGDNYPAYNVSWYAILK"
     gene            complement(637..1077)
                     /locus_tag="PLB85_04820"
     CDS             complement(637..1077)
                     /locus_tag="PLB85_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU72686.1"
                     /translation="MRTDRMNLTDHEIQNSLVRLQHFLLQLDENINWDRVKKTLEPLD
                     PSRKNVAGHDSYEPLKMFRIMLLQSWYDLSDPEMEFYLNTNLLFMSFCRFINASVMLA
                     PCFRCDQRSRARKALPCMLTNQDGRGWQMIFELTEFTESDIAFK"
     gene            1334..1621
                     /locus_tag="PLB85_04825"
     CDS             1334..1621
                     /locus_tag="PLB85_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72687.1"
                     /translation="MQITLSGNSAVITWDAVTENILDQPITLSGYYIYKSTDPLGGFT
                     YQGSSSVNQYILPTTAISHSRMFYHVTAFKQYVKGGFDPAAPGPDSGSSQE"
     gene            complement(1743..3020)
                     /gene="eno"
                     /locus_tag="PLB85_04830"
     CDS             complement(1743..3020)
                     /gene="eno"
                     /locus_tag="PLB85_04830"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOU72688.1"
                     /translation="MSEILYIKGREILDSRGNPTVEADIHLESGVVARAAVPSGASTG
                     EREAIELRDGDKSRYGGKGTLKAVDNIDRFIAPALCGLESTHQPELDQVMLNLDGTHN
                     KEKLGANAILAVSMAAARASAIEMDIPLYRYLGGVGAVTLPVPMSNIINGGSHADNNV
                     DIQEFMIMPLGARSFREAIRVNAEVFHALKKILNDRGLATGVGDEGGFAPNLENNEAA
                     LKAIMDAITAAGYKPGEDVFIALDAAASSFWIDGKYAFEGKLASNDEMIAYWVDMVAK
                     YPIVSIEDGLAENDWEGWIKLTAALGDKIQLVGDDLYVTNPAIIKRGIAEKASNSVLI
                     KLNQIGTVMETIDAINTAHKAGWTCVVSHRSGETGDTFIADLAVAMNTGQIKTGSISR
                     SERVDKYNQLIRIEEELGAAAIFPGKAVIKQLG"
     gene            complement(3047..>3659)
                     /locus_tag="PLB85_04835"
     CDS             complement(3047..>3659)
                     /locus_tag="PLB85_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HOU72689.1"
                     /translation="LYFQVTDPYKAVYEIGNLPEAIEKLTQTSLRNVIGELTLDFTLT
                     SRDTINAKLRDILDDATDKWGVKVNRVELQEILPPEEIRTAMEKEMRAERDKRAKILQ
                     ADGEREYQVRVADGERLAKIARAEGEAQAKLLVADAEKKSIALIAEAVKGSGTDPAQY
                     LVALKYVSAFREVTKQGDKTVVVPYETSALLGSVKTLANIFQK"
BASE COUNT          763 a          899 c         1082 g          915 t
ORIGIN      
        1 tttgaggatg gcgtaccagg aaacattata ggcgggatag ttatccccga ggccaaaact
       61 gcttgtccag cctgaacccg gctgcatata ctgggaatac tccgcctggg taacctcata
      121 tttgccgatg tagaaagagt tcagggtgac cgtatgggtt ggcagttcat catccgggtc
      181 tccctgccct cttgtatcac ccatggtgaa ggtgccgccg gtgactaaga caaaattctc
      241 gggtggtgga ggcagttcaa tggtgtagct tgctgtggcg atggcgctgt cctgcatgcc
      301 ggttctataa gctttggctt tcaaggttat ggtttctgaa accaggatgg gaccagcgta
      361 aactggtgta ttcgagtcag ggtcgctgcc gtcggtggtg taaaggatgg tcgtgccaag
      421 ggtagaggtg gtgatgacaa cttcaaccgc gctatcataa gtgcctccgg gaggatcgaa
      481 gaccggcttg gacaccttat ttggatcagt cggtttgtgg cagccagaca aaacaaacag
      541 aaagacgaga actgctacag aaaaaacaaa aaacaaacgt gttttcattt tatactccta
      601 ataggttagt ctatacttaa catgctttat catttttcac ttgaacgcga tatctgattc
      661 agtaaattct gtcaactcaa aaatcatttg ccatcctcgc ccatcctgat ttgtcagcat
      721 acagggcagt gcttttctgg ctctggatcg ttgatcacat cgaaagcaag gagcaagcat
      781 gaccgaagcg ttgatgaacc ggcaaaaact catgaacaaa agattggtgt tgagatagaa
      841 ctccatctcg ggatcagaaa gatcatacca tgattgcagc agcatgatcc tgaacatctt
      901 gaggggttcg tatgaatcgt ggccggcaac attcttacgg ctgggatcaa gaggctccag
      961 ggtcttcttg accctatccc aattgatgtt ttcatccaac tgaagaagaa agtgttgcag
     1021 tctgaccagg ctgttctgga tctcatgatc ggtcaggttc atcctgtctg ttctcatgct
     1081 aaactccatc aacgtaatag agtgacaata tatagggaca actatcctgt caggaattaa
     1141 ttacactatt cctgacctat catctatgtc gttcgcttca tggttgggat ttaacttccg
     1201 tgacttatgt gcaaaggtct ttaatagtgc cactttcggc agccatagac taacggcaag
     1261 gctatggaag gatatctttg tcgccaagtt atctcccata gatgaagagg ctccgcccaa
     1321 agcgccggag aatgtgcaaa taacgttgag cgggaacagc gccgtgatca cttgggacgc
     1381 ggtgacagaa aacatcctcg accagcccat cacgctaagt ggttactaca tttacaaatc
     1441 cacagatccc ctgggagggt tcacatacca agggtcgtcc agcgtcaatc aatacatcct
     1501 tcccacgaca gccatttccc attccaggat gttctaccac gtgacggcgt tcaaacaata
     1561 cgtcaagggt gggtttgacc ccgcggcgcc gggaccggat tccgggtcga gccaggaatg
     1621 acgtggatga aaactgtctt gacgccgtaa tgacaggggg aaaacagaaa gggcgggaag
     1681 aataatcccg cccctatggc aaaggcttga tccgcaggct gaaagggcca gcctagctgg
     1741 aatcagccga gttgtttgat cacggctttg ccggggaaga tggcggccgc gccgagctct
     1801 tcctcgatgc ggatgagctg gttgtatttg tccacgcgtt cgctgcggga aatggagccg
     1861 gttttgatct ggccagtgtt catggccacg gcgaggtcgg cgatgaaggt gtctccggtt
     1921 tcgccgctgc ggtggctgac cacgcaggtc cagccggctt tgtgggcggt gttgatggcg
     1981 tcgatggtct ccatcacggt gccgatctgg ttgagtttga tgagcacgct gttggaggct
     2041 ttttcggcta ttccgcgctt gatgatggcg gggttggtta cgtagaggtc gtcgcccacg
     2101 agctggattt tgtcgcctag ggcggcggtt agcttgatcc agccttccca gtcgttttcc
     2161 gcgagtccgt cctcgatgga gacgatagga tatttggcca ccatgtccac ccagtaggcg
     2221 atcatttcgt cgttggaggc gagttttccc tcgaaagcgt atttgccgtc aatccagaag
     2281 ctggaagcgg cggcgtcgag ggcgatgaag acgtcttcgc cgggtttgta gccggcagcg
     2341 gtgatggcgt ccatgatcgc tttgagggcg gcttcgttgt tttccaggtt gggcgcgaat
     2401 ccgccttcgt caccgactcc agtggcaagg ccacggtcgt tgagtatctt tttgagggcg
     2461 tggaagacct ccgcgttcac gcggatggct tcgcggaagg atctggcgcc caggggcata
     2521 atcatgaatt cctggatatc gacgttgttg tcggcgtggg aaccgccgtt gatgatgttg
     2581 ctcatgggca cggggagggt cacggcaccc actccgccga ggtagcggta gaggggaatg
     2641 tccatttcga tggcgctggc gcgggcggca gccatggaaa cagccaggat ggcgttggcg
     2701 ccaagctttt ccttgttgtg ggtcccgtcg aggttcagca tcacctggtc gagttccggc
     2761 tggtgggtgg attccagccc gcagagggcc ggagcgatga atctatcgat gttatccacg
     2821 gctttaaggg tgcctttgcc gccgtagcgg gatttatcgc catcccgcag ttcgatggct
     2881 tcgcgttcac cggtggaggc accgctgggc acggcggcgc gggcgaccac accgctttcc
     2941 agatggatgt cggcttccac ggtggggtta ccgcgtgaat ccagtatttc ccttcccttg
     3001 atgtaaagaa tttctgacat tgggttatgc tccttggatg tgttatttac ttttggaaaa
     3061 tgttggccag ggtcttcacg gaaccgagca gggcggaggt ttcgtaagga accacgacgg
     3121 tcttgtcacc ctgtttggta acttcccgga aggctgacac atacttgagg gccacgaggt
     3181 attgggcggg gtcggttccg ctgcccttca ccgcttcggc gatgagggct atggatttct
     3241 tctcggcatc ggccaccagc agcttagcct gggcttcgcc ttcggctcgg gcgatcttgg
     3301 caagtctttc accatcggcc acgcgcacct ggtattcgcg ttcaccgtcg gcctggagga
     3361 ttttggcacg tttgtcgcgt tcagcgcgca tctctttttc catggcggtg cgaatctctt
     3421 ccgggggcag gatctcttgc agttccacgc ggttgacctt cactccccac ttgtcggtgg
     3481 catcatcaag gatgtcgcgc agcttggcgt ttatggtgtc gcgcgaggtg agggtgaaat
     3541 cgagggtgag ttcgccgatg acgttacgca gcgaggtttg ggtgagtttt tcgatggcct
     3601 cggggagatt gccgatctcg taaacagcct tgtaggggtc ggtgacctgg aaatagagg
//
LOCUS       DAOOWR010000167         3627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25674_length_3627_cov_3.281635, whole genome shotgun sequence.
ACCESSION   DAOOWR010000167 DAOOWR010000000
VERSION     DAOOWR010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25674_length_3627_cov_3.281635"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLB85_04840"
     CDS             <1..924
                     /locus_tag="PLB85_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72690.1"
                     /translation="QVWPIFSSLVEEDLVCLIAGYPSAPFFAQRVSPQAAFPWNEAGV
                     LIHEPHHLTVGCAIDDNTLNLIYDHTEGSGTRSIRLKRVILNGGNLGYPSPGLQLNTS
                     TPLELGKVSLARLECDALLGVWSEQSNYQDDGRDLYYRLIDPENELVGDSQSSFGSHQ
                     GDADNPRISTFGDEAIVCWSDERTGIPAVGHLQTGIYAQKLAYLSSSLPQEPDTPSAG
                     LAFVNCYPNPFRGSVQVNWSTKAAQPAELLICNIHGQVVKRFRYMLLQSGEHSLYWDG
                     NDEQGRQVSSGVYLLRLRSGNESRTVKILRW"
     gene            complement(1209..3104)
                     /gene="alr"
                     /locus_tag="PLB85_04845"
     CDS             complement(1209..3104)
                     /gene="alr"
                     /locus_tag="PLB85_04845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU72691.1"
                     /translation="MAKEAFIERSWVEIDLAAFRANLRALKAKMGAEQGFIQIVKADA
                     YGHGAPEIARISLSEGAVALGVANPEEGKLLRVQGFKEPILILSPSLPCEIPGILDHE
                     LWPTLSDTDFARELDHACAERDIRAKVCLKMDSGMHRSGILADGFTELLATLDKLPNL
                     KLQSVFSHFAASESDPDFSQAQEKEFLAILKKHKVNVPWIHLANSAAVVNGFGKSLPL
                     CRLGILSFGIYTHPSQKEKIDLKPVMTFKSTVAQLKEIPAGATVGYNRSWKATRLTRY
                     AVIPVGYADGYDFLLSNRGTVALKGTLCPVIGRVSMDMICLDVTDAGRVEIGDEVVLL
                     GNSAEEIRAENLAAAYSGSAYELLCQVGRRAKRYYYDGQRLVTSSPLSRREFVSSDYP
                     DSKLNQIIKAAISQRVNSEEMGDLISRDILRVFFFNKDREILYRRDFRHHISFSDSGD
                     LHFWRAATTLSFSKTLQNDYFTVACANSVAALKAYFKRRDVEYRWLMDSNFELSPEVF
                     SLSSVRIDGIELETKLHFRNACMEIRCSHPRLRDLVGHEVLFEIDTLTLYPRNSHQLS
                     VFITELTHGVKISFSHPAELEPIECVPIFAGQNKYPRVSTGKTNITVSTKAEEWVFPQ
                     SGVVFAY"
     gene            complement(3116..>3627)
                     /locus_tag="PLB85_04850"
     CDS             complement(3116..>3627)
                     /locus_tag="PLB85_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU72692.1"
                     /translation="TRLVTSRPIFPLGLDLGYLPGTKSDKFNPWMQPIYDNMDILLAL
                     HEDFKSDSMKGKRKPSLEDFLDYGFLELEPLTYIRGRSLPDQFIIIDEAQNLTPHEMK
                     TIITRAGENTKVVLTGDPYQIDIPYLDSSSNGLSVAVEKLKNEDIVGHITLEKGERSQ
                     LADLAAKFF"
BASE COUNT          760 a          987 c         1058 g          822 t
ORIGIN      
        1 caggtttggc cgatcttctc aagcttggtg gaagaagacc tggtctgcct gatcgcgggc
       61 tatcccagcg ctcccttctt cgcccaacgg gtctcacccc aggcggcctt cccctggaac
      121 gaagcgggtg tcctgatcca cgaaccgcac cacctcaccg tcggctgcgc catcgatgac
      181 aacaccctga acctgatcta tgaccatact gaaggatcag gaacgcgcag catacgcctg
      241 aaaagggtga ttctgaacgg tgggaatctg ggctatccat ccccaggcct gcagctcaat
      301 acatctaccc cgcttgagct tggcaaggtg agcctggcca gattggaatg cgacgccctg
      361 ctgggtgtgt ggagcgaaca aagcaactat caggacgacg gccgtgacct gtattaccgc
      421 ttgatagatc cagagaatga actggtgggc gattcccaat cgagctttgg ctcccatcag
      481 ggcgacgcgg acaatcccag gatcagcact ttcggcgacg aggctatagt ctgctggagc
      541 gacgagagga cgggaattcc cgctgtcgga caccttcaga caggtatcta tgcccaaaag
      601 ctggcttacc tgtccagttc ccttccccag gaaccggata ccccctcagc tgggctggcc
      661 tttgtcaact gctatcctaa ccccttccgc ggcagcgtgc aggtgaactg gtcaaccaaa
      721 gccgcccagc cagcggaatt gctgatctgc aacattcacg gacaggtggt gaaacgcttc
      781 cgctatatgc tgttgcagtc gggagaacat tccctctatt gggatgggaa tgacgagcaa
      841 ggccgccagg tgagttccgg agtgtatctg ctgcgcctca ggagcggcaa tgaaagccgc
      901 actgtcaaga tcctacgctg gtagccactt cgaaaggaca ttattacaag agggaagcgc
      961 ctgctttcct ctttttcatc ttgctgtttt tcggctctcc atcaagctaa acgggtttat
     1021 catcggttgc gtatatttgc tttatgcaat tactcaaccc cgggcttggg tcgaatgaga
     1081 aaaggggcgg cacagcgacc gtctgtcaga tgctaccact cgcctgaagc accaccaaaa
     1141 ggaaaaagga ggaaagagct tcgtcggcag caaacccatc tgtagcccgt gctcgccaga
     1201 ctccaggatc aatacgcgaa caccaccccg ctttggggaa agacccattc ttcggctttg
     1261 gtggagacgg taatattggt tttgcctgtg gaaacccgcg gatacttgtt ctggccagcg
     1321 aatatgggca cgcactctat cggttccagt tctgcagggt gggaaaagct gatcttcaca
     1381 ccgtgggtga gttcagtgat gaaaacgctg agttgatgcg agttgcgggg atagagagtg
     1441 agggtgtcga tctcgaacag gacctcgtgg ccaaccaggt cccgtagtcg ggggtgggag
     1501 cagcgaatct ccatgcaggc gtttctgaag tggagtttgg tctccagttc gattccatcg
     1561 attctcactg aggagaggga aaaaacctcg gggctgagct cgaaattgct gtccatcaac
     1621 cagcggtatt ccacatcacg gcgcttgaaa taagctttca gggctgccac ggaattggcg
     1681 caggccacgg tgaaatagtc attttgcagc gttttgctga agctgagggt ggtggcagcg
     1741 cgccagaagt ggaggtcgcc ggaatccgag aagctgatgt ggtggcggaa gtcgcgccgg
     1801 taaaggattt cgcggtcttt attgaagaaa aacaccctca ggatatccct tgagatcaga
     1861 tcgcccattt cctcgctgtt cacgcgctgg ctgatggcgg cctttatgat ttggttgagc
     1921 tttgaatccg gatagtccga agagacgaat tcccgccgcg agagggggga ggaggtcacc
     1981 agccgctggc cgtcataata atagcgcttc gcgcggcgcc ccacctggca gagcagctca
     2041 taagccgatc cggaatatgc agcagcgagg ttttccgccc ttatttcctc agcggaattc
     2101 cccagcagca ccacctcgtc gcctatttcc accctgccgg cgtcggtgac gtccaggcag
     2161 atcatgtcca tggaaacccg cccaataaca gggcagagcg tgcctttgag ggccacggtc
     2221 ccgcggtttg agagcagaaa gtcatagccg tcagcgtagc ccaccggaat tacggcgtag
     2281 cgtgtgagcc gggtggcttt ccaggagcgg ttgtagccaa ctgtcgcgcc ggcgggtatc
     2341 tccttaagct gcgccaccgt cgatttgaaa gtcatcacgg gtttaaggtc tatcttttcc
     2401 ttctgggagg ggtgagtgta aattccaaag ctgaggatgc ccagacggca gaggggaagc
     2461 gatttcccaa agccgttcac cactgccgcg ctgttagcca gatggatcca gggtacgttc
     2521 actttgtgct ttttgaggat ggccagaaat tctttttcct gagcctggct gaaatccggg
     2581 tcgctctcgc tggcagcaaa gtgggaaaac acgctctgca acttgagatt gggcaacttg
     2641 tcaagcgttg ccagcagctc tgtgaaacca tctgccaaaa tcccgctgcg gtgcatcccg
     2701 ctgtccattt tgaggcacac cttcgccctg atgtcgcgtt cagcgcaggc atggtcaagc
     2761 tcgcgggcaa agtcagtatc gctcagggtg ggccagagtt catggtccag tatccccgga
     2821 atctcgcagg gaagcgaagg tgagaggatc aggatgggtt ccttgaagcc ctgcacccgc
     2881 aacagttttc cctcttctgg attggccacg cccagcgcca cagctccctc ggagagggag
     2941 atccgggcga tttctggcgc gccgtgcccg taggcgtcag ctttcacgat ttggatgaag
     3001 ccctgttccg cgcccatttt ggctttcagg gctctcaggt tggcgcggaa agccgccagg
     3061 tcgatttcca cccaactgcg ttcgataaat gcctcttttg ccatttcgcg cgctcctaaa
     3121 agaacttggc agcaaggtcc gccagttgcg aacgctcgcc tttttccagg gtgatatggc
     3181 ccacgatgtc ctcgttcttc agtttctcca cggctacgga aagcccgttg gaagaggaat
     3241 ccaggtaggg gatgtcgatt tggtaggggt cgcctgtgag caccactttg gtattttccc
     3301 cggcgcgggt gatgatggtt ttcatctcgt gcggggtgag gttttgggcc tcgtcgatga
     3361 tgatgaactg gtctggcagg ctgcgtccgc ggatgtaggt gagcggttcc agttccagaa
     3421 atccgtaatc caggaaatct tccaggctgg gcttgcgttt gcctttcatg ctgtcgcttt
     3481 tgaagtcctc gtgcagcgcc agcaggatat ccatgttgtc atagattggc tgcatccagg
     3541 ggttgaattt gtcgcttttg gtgccgggaa ggtagccgag gtccagtccc agcgggaaaa
     3601 tcggccgcga tgtcaccagc cgggtgt
//
LOCUS       DAOOWR010000168         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25876_length_3606_cov_3.798648, whole genome shotgun sequence.
ACCESSION   DAOOWR010000168 DAOOWR010000000
VERSION     DAOOWR010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25876_length_3606_cov_3.798648"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLB85_04855"
     CDS             <1..549
                     /locus_tag="PLB85_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOU72693.1"
                     /translation="VPVGERMLWLHLFPFLWKVNLGQANWEFGSKLVTYESGGYSFSP
                     SICYELAFPEVFHRMAMPRDSLSGSYSKCDYLANITNDAWFGTSYGLWLHAMMSRFRA
                     VENRIQIYRSANTGISMVVDPKGRILAKTGLNEVTTITAPLYTTPKIPLIRRIYRYPF
                     LFVALAAILALTARIRKPGGKP"
     gene            546..926
                     /locus_tag="PLB85_04860"
     CDS             546..926
                     /locus_tag="PLB85_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU72694.1"
                     /translation="MKKYFYTIGEVSNLLGVKPYVIRYWESEFSFLRPRREEGRIRKY
                     SEENIELLRRIQDMLHNQRYTIEGARQKLKEERRSSRSARPVATAQTQEIRHPDELKQ
                     LLRGLRSSLDQIRCACKQLRREAK"
     gene            923..1765
                     /locus_tag="PLB85_04865"
     CDS             923..1765
                     /locus_tag="PLB85_04865"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOU72695.1"
                     /translation="MNFQDIILTLQTYWAGQGCSLMQPYDTEMGAGTFHPSTFFGALG
                     PKPVSVAYAQPCRRPKDGRYGKNPNRLQHYYQFQVIIKPSPDDIQNLYLRSLEAIGIE
                     IAKHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVAGIDCFPVSVELTYG
                     LERLAMYIQNVDDYRQLRWNDTLLYGDYFFAQEQEYSDYNFNAADTALLFKLFEDYEK
                     ECRKLLEKMLVYPAYDNLLRCSHTFNLLDARGVISVTERAAYIGRIRNLARETAGTYM
                     GKYN"
     gene            1879..2586
                     /locus_tag="PLB85_04870"
     CDS             1879..2586
                     /locus_tag="PLB85_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72696.1"
                     /translation="MKKSLLLCLLPLLLLASCHVHVVDTPFLAKFENMLYTDIRVEVD
                     GYGSAVIAPGESVTFEIARDDRSYHYEAETHGKSLSGEQIGLVVEWSRTRNISGDSYT
                     TYLITNEDLFFLKMRNTGHHGLHPLYVNYGLTDQTMDDITIPGNGVLYNTGYYKAFTN
                     TRVQANWLDMPSDYTYWEQGHHFNFPWKENQSVTLLNTFKKDAAPEQGFKVESNLVDP
                     RSDEPFGIDAGTPRGNN"
     gene            2591..3388
                     /locus_tag="PLB85_04875"
     CDS             2591..3388
                     /locus_tag="PLB85_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOU72697.1"
                     /translation="MLRSSITLGNSDLPLMATAIHNGHHLPPELLAISGISEADRLRE
                     EDPFTGEIAALFPNHIIVESSRFAIDLNRNPGKAVYLAPEDCWGLPVRAEPVPEPVLA
                     RLREDYASWYKLLRYQIDRMLEFHPFLVVLDIHSYNHRRGGPNAEPDPQDENPDIIIG
                     RGNLLQKHYPAAEALCQMLNGKDYLNKALDCRQDVKFTGGYMSRWLNSRYPDRLICLA
                     IEFKKIFMDEWTGELDQPAWSELKLLFHSQTRDWISAQYGWTLSPLA"
BASE COUNT          886 a         1065 c          913 g          742 t
ORIGIN      
        1 gtcccggtgg gcgaacgcat gctctggctg catctttttc cctttttgtg gaaagtgaac
       61 ctcggccagg ctaactggga atttggctct aagctggtca cttatgaaag cggcggctac
      121 agcttttccc cctccatctg ctacgagctg gcctttccgg aggtctttca tcgcatggcc
      181 atgccgcggg acagtctcag cggaagttac agcaaatgcg attacctggc caatatcacc
      241 aacgacgcat ggttcggcac ttcctacggt ctctggctac acgcgatgat gtcacgtttc
      301 cgtgcggtgg aaaacaggat ccagatctac cgtagcgcca acaccggaat ctcgatggtt
      361 gtggacccaa aaggcagaat cctggccaaa accgggctga acgaggttac tacaatcacc
      421 gcgccgctct acaccacacc caaaataccc ctcatccgta gaatttaccg ctaccccttc
      481 ctctttgtgg cgctcgcggc cattctggcc ctgaccgcca ggatcagaaa accgggaggc
      541 aaaccatgaa aaaatacttt tacaccatcg gcgaggtgag caacctgctg ggcgtaaaac
      601 cttatgtgat ccgctattgg gaaagtgaat tcagctttct gcgcccgcgc cgggaggaag
      661 gccgcatacg caagtattct gaagaaaaca tcgaacttct gcgcagaatc caggacatgt
      721 tgcacaacca gcgttacacc atagaaggcg cgcgccagaa gctgaaagag gagcgccgtt
      781 cctcacggtc agcccggcca gttgcgacgg ctcaaactca agaaatcagg cacccagatg
      841 agctaaagca gcttctgcgg ggcctgcgtt cctcgctgga ccagatcagg tgcgcttgca
      901 aacaactcag gagggaagcc aaatgaactt tcaggacatc atcctcaccc tgcaaactta
      961 ctgggcaggc caaggctgca gcctgatgca gccatacgac accgagatgg gggctggcac
     1021 cttccatccc tcaacctttt ttggcgccct gggtcccaaa cccgtatcag tggcttacgc
     1081 ccagccctgc cgccgtccaa aggacggccg ctacggcaaa aatcccaacc gacttcaaca
     1141 ctactaccag ttccaggtga tcatcaaacc cagtccggat gacatccaaa acctctatct
     1201 gagaagcctg gaggccatcg gcatcgagat agcaaaacac gatatccgct ttgttgaaga
     1261 cgactgggaa tcccccaccc tcggtgcctg gggcttagga tgggaggttt ggctggacgg
     1321 catggagata agtcaattca cgtatttcca gcaggttgcg ggcatcgatt gcttcccggt
     1381 gagcgtggaa ctcacctacg gtttggaacg cctggcgatg tacattcaga acgtggacga
     1441 ctaccgtcaa ctgcgctgga acgacacgct gctttacggg gattactttt tcgcccagga
     1501 gcaggaatat tccgactaca acttcaacgc ggcagacact gccctgcttt tcaaactttt
     1561 cgaggactac gaaaaggaat gccggaaact gctggagaaa atgctggtgt atccagccta
     1621 cgacaacctt ttgcgctgtt cccacacttt caatctgctg gacgcccgcg gcgtaataag
     1681 cgtaaccgaa cgcgccgctt acatcggcag gatcaggaac ctggccaggg aaaccgccgg
     1741 aacctacatg ggcaaataca actgagacat tccgaaaatt cggatacagg atttgccccg
     1801 cggcctggta ttaataatga gcagcttgta accagcgctt gatagaacgc ttggattttg
     1861 gctgccaagg agtccaaaat gaagaaatct ttgcttctct gcctgttgcc actgctactg
     1921 ctggcttcgt gccacgtcca tgtggttgac accccttttc tggccaaatt tgaaaacatg
     1981 ctttacaccg atatccgtgt ggaagttgac ggttacggtt cggctgtgat cgcccccggg
     2041 gaaagtgtga ctttcgaaat cgcccgggac gaccgaagct atcattacga agctgaaacc
     2101 cacggcaaga gcttaagcgg cgagcagatc ggcctggtgg tggagtggtc gcgcacccgc
     2161 aacatcagcg gagattccta caccacctat ctgatcacca atgaagacct ctttttcctg
     2221 aaaatgcgca ataccggcca tcacggcctg catcccctct acgtgaacta cggactgacc
     2281 gaccagacca tggacgacat caccatccca ggcaacggag tgctctacaa caccggctac
     2341 tacaaggctt tcacgaacac ccgcgtacag gctaactggc tggacatgcc ctccgattac
     2401 acctactggg aacagggcca ccatttcaac tttccgtgga aagaaaacca gtctgtaact
     2461 ctactgaaca cgttcaagaa agacgccgcc ccggaacagg gattcaaagt ggaatccaat
     2521 ctggtggacc cccgctcgga cgagcccttc ggcatcgacg ccggaacccc aagaggcaac
     2581 aactgatccg gtgctgagat cttccataac gcttggcaac agcgacttgc cgctgatggc
     2641 cacggctata cacaacggcc atcacctgcc cccggaactg ctggctatca gcggcatcag
     2701 cgaagccgac cgcctccgcg aggaagaccc cttcaccggc gaaatcgctg cgctttttcc
     2761 caaccacatt attgttgaaa gctcccgctt cgccatcgac ctgaaccgca acccgggaaa
     2821 ggcggtgtac cttgctccag aggactgctg gggcctgccg gtacgcgcag aaccggtgcc
     2881 ggaaccagtt ttggcaaggc tgcgtgagga ttacgcctcc tggtacaaac tcctccgata
     2941 ccaaatcgac cggatgctgg aattccaccc ctttctcgtg gtgctggaca ttcacagcta
     3001 caaccaccgc cgcggcgggc ccaacgctga gccagacccc caggatgaaa atcccgatat
     3061 tatcatcggc cgcggaaacc tattacagaa gcactacccc gcggcggaag ccctttgcca
     3121 gatgctcaac ggcaaagact acttgaacaa agcgctggat tgtaggcagg atgtgaagtt
     3181 caccggtgga tacatgtccc gttggctaaa ttcccgctat ccagaccggc tcatctgcct
     3241 tgccatagag ttcaaaaaga tcttcatgga cgaatggacc ggagaactgg accagccggc
     3301 ctggtcagag ctcaagctac tgttccatag ccagacgcgg gactggattt ccgcccagta
     3361 cggttggacg ctgtcccctc tggcatgact gggattcttc cgataaggca gcagaattgt
     3421 ttgcatagag gaagttggct aaaacaggct taattggtga tgataccata ctgtctataa
     3481 gcagccaagg agtctgccta cgttatcaag agcaggctcc cgactcgggg tggataattt
     3541 tcattgacag atttcccctc ccaaggattt tgcccctacg atgcgttccg gaatagctca
     3601 gcggta
//
LOCUS       DAOOWR010000169         3604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25896_length_3604_cov_3.571429, whole genome shotgun sequence.
ACCESSION   DAOOWR010000169 DAOOWR010000000
VERSION     DAOOWR010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25896_length_3604_cov_3.571429"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..1476)
                     /locus_tag="PLB85_04880"
     CDS             complement(193..1476)
                     /locus_tag="PLB85_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU72698.1"
                     /translation="MDKKETQYYHALRILEDNCTGCTACVRVCPTEAIRVRNRKARID
                     ANRCVDCGRCLETCEFHAIIPRSDPLDIIHRFKYKVAVVSTSYFGQFTEDVSYEVAKQ
                     AIYELGFDEVQEEAMVTEFMIGMIRDYIRHKRNTRPILSSNCPTVVRLIQLKYPSLLP
                     NLFHLEAPMSILTSYLRDRIKAETGLAEEDIGIFLIVPCPAHVTAVHQPEGAYKHLQD
                     GAFSIGMIYGRVHEHIKKLQNHPPRIQTFPKGLTWALSGVQADLVDCEDIRALSVNGV
                     DNVMEILSRVEDHYLDQYDYIVLRSCTNGCVGGSFNVENPFVAMSRIKKMIKESTNSE
                     ADVHELDNLYRAGEFDVVPLTPRSIMELDKDIKTAIFKMKRINEILTSLPGLNCSACG
                     SPTCYALAEDIVQGKATIDDCVVLLKRHSKEEEQE"
     gene            complement(1469..1969)
                     /locus_tag="PLB85_04885"
     CDS             complement(1469..1969)
                     /locus_tag="PLB85_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72699.1"
                     /translation="MAEFWHIHEGFEEAVKKHFAEPPEADVSLDSLIPEKDFNSAGKG
                     SADVKNLLKRLGVNPEILRRVAVAAYEAEINVAAHSSGGAMLSHVYPDLIHMIFQDNG
                     PGIGNIEQAMVPGYSTADDMVREMGFGAGLGLPNIKKNTDALHISSAAGDTTTLEFLI
                     FFRQDG"
     gene            complement(1971..2318)
                     /locus_tag="PLB85_04890"
     CDS             complement(1971..2318)
                     /locus_tag="PLB85_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU72700.1"
                     /translation="MTLKDIVQLLDAEVLTPEADLDVEVPCAFSSDLISDILMCTKEP
                     TLLLTGLTNNQVIRLSDMIDIVGIVFVRGKRPLDEVIEMARERSLPLFCSNLTMYRSS
                     GLLFNAGLRSCKI"
     gene            complement(2773..>3604)
                     /locus_tag="PLB85_04895"
     CDS             complement(2773..>3604)
                     /locus_tag="PLB85_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72701.1"
                     /translation="QYGDDGAGGFYPMMFSDWPFPHWDTSAHNDAMMFHYNGVKVSEP
                     NDQGMMVCIWQDSQRARMYNYYSDTDYINFAQTPEIYISVTPDNGNTWSEPIILNNVE
                     TPQLAGIKPMYVYPADKVIYTGMQGPNKVGKIGVMFYNDFTWGSNVNSPPYHNTPDGG
                     EVMFMELKIVFPLGSAADDPTMPSVTRLLLQNYPNPFNPETTICFDMPASGPARLEIY
                     NVRGQMVKTLAEGNHEFGRHSYVWNGTDNGGNSVSSGIYFYRLTTSSHSESRKMMLMK
                     "
BASE COUNT          706 a          914 c         1091 g          893 t
ORIGIN      
        1 cggtgttcag gtgcttcatg atggttatcc aggcctggcc ttcccgggcg gagcccatca
       61 cgtcgctgag catgtcgcaa acgtagccgc cgctcaggga aacctgttcg gggtcgactt
      121 ggggggtgtg gttggtgcct tcgatggcgg tcatgaaatc tgccagtgtt atcgtcatct
      181 ctgtatcctg tgtcattcct gttcttcttc tttggagtgg cgcttgagca gcaccacgca
      241 gtcgtcgatg gtggctttgc cctgcacgat gtcctctgcc agtgcgtagc aggtcggcga
      301 gccgcaggcg ctgcagttca ggcctggcag ggaggtgagt atctcgttta ttctcttcat
      361 cttaaagatg gctgtcttga tgtctttgtc cagttccatg atggagcgcg gggtcagggg
      421 caccacgtcg aattccccgg cgcggtaaag gttgtccagc tcgtgcacgt ctgcctcgct
      481 gttggtgctt tcctttatca tctttttgat gcggctcatc gccacgaagg ggttttccac
      541 gttgaaggaa ccgcccacgc agccgttggt gcagcttcgg agcacgatgt agtcgtattg
      601 gtccagatag tggtcttcca cgcgggaaag gatttccatc acgttgtcca cgccgttcac
      661 ggaaagcgcc ctgatgtcct cgcagtcaac cagatcggcc tgcacgccgg aaagcgccca
      721 ggtgaggcct ttgggaaaag tttgtatccg gggcggatga ttttgcagtt ttttgatgtg
      781 ttcgtgcacc ctgccgtaaa tcatgccgat ggagaaggcg ccgtcctgaa ggtgtttgta
      841 ggctccctcc ggctggtgca cggcggtcac gtgggcgggg cagggcacga tgaggaaaat
      901 tccgatatcc tcttctgcca ggccggtttc cgccttgatc ctgtcgcgca ggtaactggt
      961 gaggatgctc atcggggctt cgaggtggaa gaggtttggc agcagcgagg ggtatttgag
     1021 ctggattagc cgcaccacgg tggggcaatt gctgctcagg atcggccgcg tgttccgttt
     1081 gtggcggatg tagtcgcgga tcatgccgat catgaattcc gtgaccatag cctcctcctg
     1141 cacttcgtca aagcccagtt cgtagatggc ctgcttggcc acttcatagg aaacgtcttc
     1201 ggtgaactgc ccgaaatagg aggtggaaac caccgccacc ttatacttga acctgtgtat
     1261 tatgtccaga gggtcgctgc ggggaattat agcgtggaat tcacatgttt ccaggcatct
     1321 gccgcagtca acgcagcggt tggcgtcgat tcgcgccttg cggttgcgca cccggatggc
     1381 ctctgtgggg caaaccctca cgcaggcggt gcagccggtg cagttgtctt ccagtatccg
     1441 gagcgcgtgg tagtattgtg tttccttttt atccatcttg cctgaaaaag atcaaaaact
     1501 ccagcgttgt ggtgtcgccg gctgccgaac tgatatgcag cgcgtcggtg ttctttttga
     1561 tgttgggcag gcccagcccg gctccgaatc ccatttcccg gaccatgtcg tccgcggttg
     1621 aatagcccgg aaccatcgcc tgttcgatgt ttccaatccc cggcccgttg tcctggaaga
     1681 tcatgtggat gaggtctgga tagacgtggc ttagcatcgc cccgcccgat gagtgtgccg
     1741 cgacgttgat ttcggcttca taggccgcaa cggccacccg gcgcaggatt tccgggttca
     1801 cgcccaggcg cttgagcagg tttttcacat ctgccgaacc ttttccggcg ctgttgaaat
     1861 ccttttccgg aatcagcgaa tccagggaaa cgtcggcttc gggaggctcc gcgaaatgtt
     1921 ttttcacggc ttcctcaaag ccttcgtgga tatgccagaa ttctgccatc tcagattttg
     1981 cagcttcgca gcccggcatt gaacagcaag ccgctggaac ggtacatggt taggttgctg
     2041 cagaatagag gcaggctgcg ttccctggcc atctctatta cttcgtccag ggggcgtttg
     2101 ccgcgcacga acacgatgcc cacgatgtcg atcatgtccg aaagcctgat gacttggttg
     2161 ttggtaaggc cggtcaaaag cagggtcggt tctttggtgc acataagtat gtcgctgata
     2221 aggtcggacg agaaggcgca gggcacctcc acatccaaat cggcttcggg ggtcagcact
     2281 tccgcgtcga ggagctgtac gatatccttt aatgtcattc actatcacct gttcatattg
     2341 atgacgccac tacaattcaa aggccataca agtcaagcaa ataattgcgc cccagatttg
     2401 gcgtagcgga caatgctgtc ggtcgctgaa tcgcttttct tccccatttt aaccaggtgt
     2461 atgcagaaaa cagctcggag ggcagtagaa accaatcccc gcgaaaaagc gaagcgacgc
     2521 cgactggccg ttaaggccct gtcatgtgta tcgggtgtgc ctcccgcacg atgcccgcat
     2581 ttgatgcgag tatttgtcgg gaggcgtggg agtgggatgg tagtgagcgc taagggcgtg
     2641 acagtcagga cgggtcgcgg aaagtacgca atgaacggac actgaagctg acattggaaa
     2701 agcaagaaaa aagggacggc cgaaatggcc gtccccggat atttgaaccg gataaattcc
     2761 ggttggttgg atttatttca tcagcatcat tttgcggctt tcgctatggc tgctggtggt
     2821 gaggcgatag aagtagatgc cgctggagac gctgttgccg ccgttgtcgg ttccgttcca
     2881 gacatagctg tggcggccga attcatggtt gccttcagcc agggttttca ccatctggcc
     2941 tctgacgttg tagatctcga ggcgggcggg cccggaagcc ggcatgtcga aacagatggt
     3001 ggtttcaggg ttgaaggggt tgggatagtt ttgcagcaga agccttgtca cggagggcat
     3061 ggtaggatcg tcggcagcgg aaccaagcgg gaaaacgatc ttgagttcca tgaacatcac
     3121 ttcgccgccg tccggggtgt tatggtaggg gggagaattc acgtttgagc cccaagtgaa
     3181 gtcattgtag aacatcacgc cgatcttgcc aaccttattc gggccttgca tgccggtgta
     3241 gatcactttg tccgcgggat aaacgtacat cggtttgatg ccggccagtt gaggggtttc
     3301 cacattgttc aggatgatgg gttcgctcca ggtgttgcca ttgtcagggg taactgaaat
     3361 gtagatttcg ggggtctggg cgaagttgat ataatcggta tcggagtagt aattgtacat
     3421 ccgggcgcgt tgggaatcct gccagatgca aaccatcatg ccctgatcgt tgggctcgga
     3481 gactttcaca ccattgtagt ggaacatcat cgcgtcgttg tgggcagatg tatcccagtg
     3541 ggggaaaggc cagtcagaga acatcatcgg atagaatccg cctgctccgt catcgccgta
     3601 ttgg
//
LOCUS       DAOOWR010000170         3601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_25923_length_3601_cov_3.158770, whole genome shotgun sequence.
ACCESSION   DAOOWR010000170 DAOOWR010000000
VERSION     DAOOWR010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25923_length_3601_cov_3.158770"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLB85_04900"
     CDS             complement(<1..283)
                     /locus_tag="PLB85_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72702.1"
                     /translation="MTRSGTTSILLACLLACVCCVPAAAKHSDKFLLGTYSYLRNSRN
                     SAQRVVLYRQMKELGYNSNLVETFEDNADLATMLKELDSYGLDVWISDKA"
     gene            complement(703..1653)
                     /locus_tag="PLB85_04905"
     CDS             complement(703..1653)
                     /locus_tag="PLB85_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOU72703.1"
                     /translation="MKKLSGKQLFFREAKHIVGIAIGALLLAIGYSWFLMPYNMAPGG
                     VGGLAQVINYYFRIPVGVSMIMLNIPLFIISFIFIGKSFGAKSVIGMLLSSIFTDLVN
                     IKNIASLDILNIAEHTFNFNGRVIYAYLGPEDILLSAIAGSVVLGLGLGIIFKSRGST
                     GGTDIPVALIKQKAGLSIGTGYYIVESGIILLVAILLKDPKILIWGNINLFITSKITD
                     LASEGLPYIKGVYVISPKVDEIKEDIYSELDRGVTFFKALGGYKKKETEVLFCVINRR
                     QVPLLTDIVKDIDPDAFMIVTDVYDVLGYGFRSRKINLSE"
     gene            1775..3436
                     /locus_tag="PLB85_04910"
     CDS             1775..3436
                     /locus_tag="PLB85_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOU72704.1"
                     /translation="MIIDLRFDGNRHSHIELDAAIPIRKILKGTDVPAERILSYKIDH
                     VHYVNDEYVPTQNTMVDCISYNHNEGSRIYQDSVIFILAKAMNAILGAGHNLVIEHSI
                     GDGVFCEVFGTQSWTPDDCQRVKEEMLRIVDSDLPIDKIRVKTSEALDIFNTMGRKDV
                     IKNLSYRYSDEVFVYRCGKYYDRFLRPLADRTGRVREFDVVYKETGFVLRFPTGRECR
                     LAHPFVLPEKLFQLHQEHDKWLDILRVHNIIDINKLTDNYEISEFILVEEALHEKKIA
                     EIAADIVKRQEVKLILIAGPSSSGKTTFAKRLSVQLQASKAKPIVIGLDDYFIDRDKT
                     VRKRNGEIDFESLHALDLEYLNKQLSQLLNGEEVELPHFDFARGKRRRSNRFIKLHEE
                     DVIVMEGIHGLNDELTAAIPANRKAKIYVSALNQLNIDNHNRVATTDCRLLRRMIRDH
                     QYRGYTATETMLRWPSVREGEEKNIFPFQENADYMFNSSLTYELGVLRKHAWKLLLSV
                     PKSSSAYTEAQRLLALLSNVNDIPDALVPYNSIIREFTNGSIFRY"
BASE COUNT          929 a          913 c          945 g          814 t
ORIGIN      
        1 aggctttatc cgatatccag acatcgaggc catagctgtc aagttccttc agcatagtag
       61 ccagatcagc gttgtcctcg aaagtttcca cgaggttgga attgtatccc agttctttca
      121 tctgccgata gagcacaact ctttgggcgg agtttctgct gttgcgcaga taggaatagg
      181 tacccaagag aaatttgtct gaatgcttgg ctgccgctgg cacacagcaa acgcaggcca
      241 gcaggcaggc cagcaggatc gaggttgttc ccgatctggt catcgtcggt ctccttgttt
      301 tggttcgatc atcagcccga agggcaacac tgttttttcg gcttatgttc atatagaatg
      361 ggtgtaactg ccgtcatccc agcgaaagaa tagcgaagca acaccatctg gccgttaagg
      421 ccctgtttcg ggttcccgag gtgcctcccg catgataccc gcatttaatg cggggataat
      481 tcaaggggcg tgcgagaggg acaaatgcga gcgctaaggg tctggctggc aggagatagc
      541 agggttagaa tctatgacgc tatttcaaat caggtggctg cagtcgctca accggaaagc
      601 agaccggcgg gctgtatcca tcctaattga acataaacct ttattagttt atcccagctt
      661 tcggtcaagt gatttttaat gcttaaggcc gggtggcttg attcactcgc tgaggttgat
      721 cttgcgcgaa cggaaaccat aacccagcac gtcataaacg tcggtaacga tcatgaacgc
      781 atcagggtcg atgtccttca cgatatccgt gagcaggggc acctggcggc ggttgataac
      841 gcagaaaaga acctcggtct cttttttctt gtaaccgccc aaggctttga aaaaggtaac
      901 gccgcggtca agttcggagt aaatgtcctc tttgatctca tcgaccttcg gcgaaatcac
      961 atacacgcct ttgatgtagg gaagaccttc tgaggcgagg tcggtgattt tggaggttat
     1021 gaacaggttg atattgcccc agatgaggat cttaggatct ttgagcagaa tggccaccag
     1081 caggatgatc ccactttcca cgatgtagta gcccgtgccg atggataatc ccgccttctg
     1141 tttgatgagg gcgacgggga tgtcagttcc gccggtggag ccacgggatt tgaagatgat
     1201 tccaagccca aggcccagca ccacggaacc cgcgatggct gaaaggagta tgtcctctgg
     1261 gccgaggtag gcatatatga ccctgccatt gaaattgaaa gtatgttcgg caatgttaag
     1321 gatgtctaaa ctggcgatgt ttttgatgtt caccaaatcg gtgaaaatgg aggaaagcag
     1381 catcccgatc actgacttgg caccgaagga cttaccgata aagatgaaac tgatgatgaa
     1441 cagggggata tttagcatga tcatggacac gccgacgggg atgcgaaaat agtagtttat
     1501 cacctgcgcg agaccgccta cgccgccagg ggccatattg tagggcatca gaaaccaaga
     1561 gtagccgatg gccagcagta aagccccaat cgcgatcccg actatgtgtt tggcttcccg
     1621 gaagaagagc tgtttaccac ttaatttctt catttttcat ttctcctaat agcttgacgg
     1681 attggcgcgc ttcagaatga tggcattttc gtgcggggtt tgtttcgggt atgagtttaa
     1741 gcccccgctg gcactattat gacaaggaat tcagatgatc atagacttga gatttgatgg
     1801 caatagacac agccatatcg agcttgacgc ggcaataccg attcgcaaga tcctgaaagg
     1861 cacggacgtc cctgccgaga ggatcctgtc ctataaaatt gaccacgtcc attatgtcaa
     1921 tgacgaatat gttcccaccc agaatacaat ggtggactgc atctcttaca accacaacga
     1981 aggcagccgc atctaccagg attcggtgat ctttatcctg gccaaggcaa tgaacgcgat
     2041 tttgggagcc gggcacaacc tggtgatcga acattccata ggagacggcg ttttttgtga
     2101 agttttcggc actcagagct ggaccccgga cgactgtcag cgagtgaaag aggaaatgct
     2161 acggatcgtg gacagcgacc tgccgataga caagatccgg gtgaaaacca gtgaggcgct
     2221 ggatatattc aacaccatgg gcagaaagga cgtgatcaag aacctgagct accgctacag
     2281 cgacgaggtt ttcgtttacc gctgcggcaa gtattatgac cgctttctgc ggcctttggc
     2341 agaccggacc ggcagggtga gggaattcga tgtggtgtac aaggaaaccg gattcgtgct
     2401 gcgtttcccc accggcaggg aatgccgcct agcccatcct ttcgtgctgc cggaaaagct
     2461 gttccaactc caccaggaac acgataaatg gctggatatc ctgcgcgtac acaacatcat
     2521 agacatcaac aaactaaccg acaactacga aatctcggaa ttcatactgg tggaggaagc
     2581 cctgcacgaa aagaaaatcg ccgaaatcgc cgcggatatc gttaaacgcc aggaagtgaa
     2641 gctgatcctg atcgccggtc cttcctcctc cggcaagacc acctttgcca aacgcctttc
     2701 agtccagctg caagcctcca aagccaaacc catcgtgatc ggccttgatg actatttcat
     2761 cgaccgggac aaaacagtgc gcaaacgcaa tggcgaaatt gactttgaat cccttcacgc
     2821 cctggacctt gagtatctga acaagcagct aagccagttg ctcaacggtg aggaggtgga
     2881 attgccgcat tttgatttcg cccgcgggaa acgtcgccgt agcaacagat tcatcaagct
     2941 gcacgaggaa gacgtgatcg tgatggaagg catccacggc ctaaacgatg aactcacagc
     3001 cgcgattccg gccaaccgca aagcaaagat ctacgtgagc gccttaaacc agctcaacat
     3061 tgacaaccac aaccgcgttg ccaccaccga ctgccgtctg ctgcgacgga tgatccggga
     3121 ccaccagtat cgtggctaca cagccacgga gactatgctg cgctggccca gcgttcgtga
     3181 aggtgaagaa aagaacatct ttccctttca ggaaaacgcg gattacatgt tcaacagcag
     3241 cctcacctac gagcttggtg tgctgcgtaa acacgcctgg aaactgcttc tttcggttcc
     3301 caaaagctcc tcggcttaca ccgaggctca gcgccttctg gctttgcttt ccaacgtcaa
     3361 tgacatcccg gatgccctgg taccgtacaa ttctatcatc cgggaattca ccaacggctc
     3421 tatcttcagg tattgaaagt gaaaagaatc catattctgg ctgatgacgt acgcaacaag
     3481 atcgccgcgg gcgaagttat cgaacggccg gcctcggtgg tgaaagaact ggtggaaaac
     3541 gccattgatg ccggagccac acaaatcacg gtggctgttg agaatggcgg caaggacctg
     3601 a
//
LOCUS       DAOOWR010000171         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26045_length_3588_cov_3.928673, whole genome shotgun sequence.
ACCESSION   DAOOWR010000171 DAOOWR010000000
VERSION     DAOOWR010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26045_length_3588_cov_3.928673"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1834)
                     /locus_tag="PLB85_04915"
     CDS             complement(110..1834)
                     /locus_tag="PLB85_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72705.1"
                     /translation="MRKLTRRLIITGLAALLLSACATSGTVAEVEPGQPNFRSIYYFL
                     AGAYHQSGADHFTADILLRKAAAEDPGAQIIRRQLLLNSIGRWQVGELPNAGLRQEFL
                     AYEKDFGLDAELLYAAIDLYDGEGDIPAAEKALARLRELYPSARADLRLFVHGLRSGK
                     GADLGLLDAARTKADEDPAILRMLAGIWFYYDPLKEKEALLRAHELDPNEASYAYLAD
                     YCVRNGDLELTRRYFSELKWPADRERMLYLAGSDQVPERNKLLIDQAETLLATGDTEL
                     LGYLAFATLMEKKAGIMDAIAAIVDTLSAPDKDKQDLYAILIAQALLRPEVKVAPERV
                     ALLTESGYFDDIVSYYVCGLSSRSPESWELEDPEAWPAFLAELDCRFPPGPAAAYLTA
                     FVQTIRDSSFTGLADAKHRLILSLGESHTLCEADYSFLLGYYQLKNNQELYLQTLRAA
                     LKDWPDNPSYCNDMGYGMLMRGEDPEQAAGFIRHALVFEPDNIYFLDSLAWYHYLTGE
                     FEEALLLMVLPQQQADLPAEIAWHIGAIYLALEDYPSARYWLQKCLETGNDPASENEA
                     REVLKLLP"
     gene            complement(1831..2898)
                     /locus_tag="PLB85_04920"
     CDS             complement(1831..2898)
                     /locus_tag="PLB85_04920"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOU72706.1"
                     /translation="MKKYILPMLALLLALAGCGRESAASNRYEKHDRLINPTKPIAYG
                     EDYEVHIFADGANLDAVKRPLESSLGREVTVVVEEKYFSVNFQEAKALNDFKPYKNLI
                     FCGTLAGKDPVSLHITKTLAPKLVSAAEASGAELFVISNHYSRDQLILYLLAKDPSTL
                     AKLAEDRSDQIFGYLLERYRRRLAMAAYQNPVIREEFFANRPFTIRIPNIYRLWKDEK
                     SGRFLSFVFQPTKPSRTKPDKYISVYYEPMPFNQVTPEWIYKTRQELGQIFMQGDYIL
                     DNKYRTEPDSIAGFKGLRMMGHWGNKEGGGFGGGFQTWAFWHAPTKTAWLVDNIVFFP
                     DGWKLPTLLELGMISQSLEIK"
     gene            complement(2895..3467)
                     /gene="pgsA"
                     /locus_tag="PLB85_04925"
     CDS             complement(2895..3467)
                     /gene="pgsA"
                     /locus_tag="PLB85_04925"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOU72707.1"
                     /translation="MRNLPNLLTLLRVLLVPVFVWLLFFSDAEHRVAWALLVFAVASF
                     TDWLDGHLARKWQVTSDFGKIADPLADKLLVLAALAALTWLEPFRLVWPIFAIIAARE
                     FGITVLREVYKHRGIIMLADKLGKLKTVLQMAGIILALSIWAWKPGLVASPAVRTTAH
                     VWFGAVALLTLASGLNYLRPGKFTPAREGK"
BASE COUNT          791 a          936 c         1091 g          770 t
ORIGIN      
        1 atcaataaaa ctgttgtatt agaagaagtg ggctgacggg gatgtcaacc ttccatgctc
       61 caccataatc gacaaggtgt aggctaccgt ccagcctttg ccaagctgtt taggggagta
      121 gtttcagcac ttcgcgggcc tcattttcgg aggcagggtc gtttccggtc tccaagcatt
      181 tttgcagcca gtaacgggca gagggatagt cttctagagc aaggtagatg gctccaatgt
      241 gccaggcgat ttccgcgggg aggtcagcct gttgttgggg cagcaccatc aggagaaggg
      301 cttcctcaaa ttcgcccgtg aggtaatgat accaggccag gctgtccagg aagtagatgt
      361 tgtcgggctc gaaaacgaga gcgtggcgga tgaatcctgc cgcctgttca gggtcctcgc
      421 ccctcatcag catcccgtat cccatgtcgt tgcagtagga agggttgtcg ggccagtcct
      481 tcagggcggc gcgcagggtt tgcagataga gttcctggtt atttttgagc tgataatagc
      541 cgagcaggaa ggaatagtca gcctcgcaaa gagtgtggct ttcgcccagg ctgagaataa
      601 gccggtgctt ggcatccgcg agaccggtga aggagctgtc gcgaatggtt tggacgaagg
      661 ctgtaagata agcggcggcc ggcccaggag gaaaacggca gtccagttct gccaggaagg
      721 cgggccaggc ctcggggtcc tccagttccc agctttcggg agagcgggaa ctgaggccgc
      781 aaacataata gctgacgatg tcatcgaaat atccgctttc cgtcagcagc gccacccttt
      841 ccggagcaac cttcacctcc ggccggagca gtgcctgggc gatgagtatt gcgtaaaggt
      901 cctgtttgtc tttgtccgga gcggaaagag tgtccacaat ggcagctatc gcgtccatga
      961 taccggcctt tttctccatc agcgtagcga aagcgagata gcccaaaagc tccgtatcgc
     1021 ccgtggccaa aagggtttcg gcttggtcga tgagcagctt attgcgctcc ggaacctggt
     1081 cggagcctgc cagatagagc atgcgttcgc ggtctgccgg ccattttagt tcggaaaaat
     1141 agcgccgggt gagttccaaa tcgccgttgc gcacgcaata gtcagccaga taggcatagg
     1201 aagcctcgtt tgggtccaat tcatgggcgc gcagcagggc ctccttttcc ttcaggggat
     1261 cgtaatagaa ccagattcca gccagcatcc tgaggatggc ggggtcttca tcagccttgg
     1321 tccgggccgc gtcaagcagg ccgaggtcgg ctccttttcc agagcgcaag ccgtgcacga
     1381 agagcctcaa atcggcacgg gcggagggat aaagttcacg caggcgggcc agtgccttct
     1441 cagcggcggg aatatcgccc tccccatcgt aaaggtcaat ggcggcgtag agaagctccg
     1501 cgtcgaggcc gaaatccttt tcataagcaa ggaattcctg gcgcagcccg gcgtttggaa
     1561 gctctcccac ctgccagcgg ccgatggagt tgagcaggag ctggcggcgg attatttgag
     1621 caccggggtc ttcagcggca gcttttcgca gcaggatgtc cgcggtgaag tggtccgctc
     1681 cgctttgatg ataggcacct gccaaaaaat agtagatgga gcggaaattc ggctggccgg
     1741 gttcgacttc cgcgacggtt ccgcttgtgg cgcaggcact tagcagcaag gccgccaggc
     1801 ccgtaatgat cagcctccga gtgagttttc tcacttaatt tccaaggatt gggaaatcat
     1861 ccccagttcc agcagggtgg gcagtttcca accatccggg aaaaagacaa tattgtcaac
     1921 aagccaggct gttttggtgg gggcgtgcca gaaagcccag gtttggaatc cgcctccaaa
     1981 gccgcctccc tccttgtttc cccagtgccc catcatgcgc aggcccttaa agccagctat
     2041 gctgtctggt tccgtgcggt atttgttatc caggatatag tcgccttgca tgaatatttg
     2101 ccccagttcc tgcctagttt tgtaaatcca ttccggggtg acctggttaa agggcatggg
     2161 ttcgtaatag acggagatgt atttatccgg cttggtgcgg ctgggttttg tgggctgaaa
     2221 gacgaaggag aggaagcgcc cggatttttc gtccttccat aggcggtaga tattaggaat
     2281 cctgattgtg aaaggccggt tggcaaagaa ttcttcccta ataacggggt tttggtaagc
     2341 ggccatggcc aggcgccggc ggtagcgttc cagcagatag ccaaagatct ggtcgcttct
     2401 gtcctcagcc agttttgcga gagtggaagg gtctttggcc aacaggtaga ggatgagttg
     2461 gtcgcgggaa tagtggttgc tgatcacgaa aagctcagcc ccgcttgcct cggcggcgct
     2521 cacgagtttc ggggcaagag tcttggtgat gtgtagcgaa acggggtcct tgccggcgag
     2581 ggtgccgcag aaaatgaggt ttttataggg cttgaagtca tttagggctt tggcttcctg
     2641 aaagttgacg gagaaatact tctcttccac cacaacggtc acctcgcggc caaggctgga
     2701 ttccaaaggc cgcttcaccg cgtctaaatt ggctccgtca gcgaaaatgt ggacttcgta
     2761 atcctctccg taggcgatgg gcttggtggg attgataagc cggtcgtgtt tttcgtagcg
     2821 gttcgaagcg gcggattccc tgccgcaacc cgccaaagcg agcagtaaag ccagcatagg
     2881 caatatatat ttcttcattt gccctccctg gccggagtga actttcccgg cctcagatag
     2941 tttaacccgc ttgccagagt aagcaaagca accgccccga accagacgtg ggcggtggtc
     3001 cggactgctg gagacgcgac cagaccgggt ttccaagccc agatggagag ggcgagaatg
     3061 attccggcca tctgcaatac tgttttcagc ttgcccagtt tgtcagcgag catgatgatt
     3121 ccgcggtgtt tgtaaacctc gcgcaggacg gtgatgccaa attcgcgcgc cgcgatgatg
     3181 gcaaagatgg gccagacgag gcgaaagggc tccagccagg tgagcgcggc cagagcagcc
     3241 agcaccaaaa gcttgtctgc caagggatcg gcgatctttc caaaatcgct ggtcacctgc
     3301 catttccggg ccagatggcc atccagccaa tcggtgaagg aagccacggc aaaaaccagc
     3361 agagcccaag ccacgcggtg ttccgcgtcg ctgaagaaga gcagccaaac gaacacggga
     3421 accagcagca cccgcagaag cgtcagcaaa ttgggcagat tacgcattgg gtggggtgga
     3481 aaccgtgatg ccacgcgttt cttgactttc cggctcgcgc atgctgttga tgagaacaga
     3541 caccaacacg gcggccagaa cagagacgaa ctgcaccgcg aagaaaga
//
LOCUS       DAOOWR010000172         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26067_length_3587_cov_3.996602, whole genome shotgun sequence.
ACCESSION   DAOOWR010000172 DAOOWR010000000
VERSION     DAOOWR010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26067_length_3587_cov_3.996602"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLB85_04930"
     CDS             <1..434
                     /locus_tag="PLB85_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72708.1"
                     /translation="LFSLPQGLCDMKKEYQEIKTTLTSDEANQMIEKVARFFVEKQMG
                     SAGILLLESLHPLHGIASQAMFFILPFAEMVFDSEKYQHFALMIQEEVHLKRLIKRID
                     ELDEELNRERRAEAKLRRQRRKNQYRAAIRKLFKKDDKSAE"
     gene            446..2308
                     /locus_tag="PLB85_04935"
     CDS             446..2308
                     /locus_tag="PLB85_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOU72709.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWVDGEYRQTIRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYNNPNIRFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMVTGVVASMTGESAERAALGAGAIVMDLIASNDKRMNHEKIERIRELRPDM
                     ILMAGGEDGGTVKHVVEMAELVSGADPKPRLGAGYKLPVIYAGNKEAQQHIKDSLADK
                     VDLIVTDNIRPKLETENLGPARDKIHDLFMEHVMKQAPGYNKLMAWCQGPDHESVPIM
                     PTPAAVGSIMQAIAKEENIEVVGVDIGGATTDVFSVFTEDFIFNRTVSANLGMSYSIS
                     NVLASAGLDNIMRWVPLDIDENYLRNMIKNKMIRPTTIPSLLEELVLEQAIAIEALRL
                     AFEQHKEFASSLKGMQRQRDISEAFSQSTSGATIVNMMTLDLLVGSGGVLSHAPRRHQ
                     TVMLLINAFLPEGVTRLAVDSIFMMPHLGVLSEISTKAATEVFRKDCMVYLGSCVAPV
                     GKSKVGKPALHARLELPDGKTWVEDIPFGEVRLIPCGVGEVAKATLRTHGGLVLDEHK
                     SKELTIDLHGGIVGIVLDTRGRQPFVIPTDRAERIATLKKWMTDFKAYPDKILK"
     gene            2318..3448
                     /locus_tag="PLB85_04940"
     CDS             2318..3448
                     /locus_tag="PLB85_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72710.1"
                     /translation="MGQAYSAGLTVTDNIILRKERILPLKGQVLVKKGDHVKAETVVA
                     ETLLPGKVVPFNLANKLGVTPAQLMNFIKIEAGQQIDTNTVLAETKGLFGTGLFKNDV
                     RSPVKGEVESISSVTGQVLLREPRIPVQVKAFIDGVVTDVMPEEGVIIENKSAYIQGI
                     FGIGGETIGEIMVISKSPDAEVTPAEITEACAGKIIVTGSFVSFDVIEAARKHGAKAI
                     ITGGIDDQDIKKLLGYDIGVAITGHENIGITIVVTEGFGKITMARKTFELLNRFNGKK
                     TSIHGRTQIRAGVIRPEIIVPLPFDETELVVKESSMPVLEPGTLIRVIRQPNFGKIAK
                     VTGLPEELTKVESETLVRVLEAQFEDGSKMILPRANVEVIET"
BASE COUNT          911 a         1043 c          976 g          657 t
ORIGIN      
        1 ggctgttctc actgccacaa gggctgtgtg acatgaaaaa agagtatcag gaaatcaaga
       61 ccacgctcac ttccgatgaa gcgaaccaga tgattgaaaa ggtggcgcgc ttctttgtgg
      121 aaaagcagat gggctcggct ggaattctgc tgctggaatc cttgcatccc ctgcacggca
      181 tcgccagcca ggctatgttt ttcattttgc cctttgctga aatggtcttc gattcggaga
      241 aatatcagca tttcgccctg atgatccagg aagaagttca cctcaaacgc ctgatcaaac
      301 gtatcgacga gctggacgaa gagctcaacc gcgaacgccg ggccgaggca aaacttcgcc
      361 gccagcgtcg taaaaaccag taccgggcgg ccatccgaaa acttttcaaa aaagacgata
      421 aatcagctga ataagcggag gtataatgca atacgacgaa aaacgcctga cccggatcgt
      481 ggccacggac tgcggcagca cgaccaccaa ggcaattttg atcgaatggg tggacggcga
      541 atatcgccaa accatccgcg gcgaggcacc cacaactgtg gaggccccgc tcaacgacgt
      601 tacaaagggc gtgatcaacg ccactcagga attggaagag ctggcccggc tcaagtataa
      661 caaccccaat atccgcttca tggaaaacgg tgaattcgtg attccgcgaa aaggcgacgt
      721 gggcgtggac gcctatgttt ccacctcctc ggccggcggt ggactgcaga tgatggtcac
      781 cggcgtggtg gcatccatga caggtgaaag cgccgagcga gccgcccttg gcgcgggcgc
      841 cattgttatg gacctcatcg ccagcaacga caaacgcatg aaccacgaaa agattgaacg
      901 catccgcgaa ttgcgtccgg acatgattct catggccggg ggcgaggacg gcggcactgt
      961 gaagcacgtg gtggagatgg cggagctcgt cagcggagct gatcccaagc cccgtctggg
     1021 tgccggttac aagctgccgg tgatatatgc cggcaacaag gaagcccagc agcacatcaa
     1081 ggattccctg gccgacaagg ttgacctcat cgtcaccgat aacatccgcc caaaactgga
     1141 aaccgagaac ctcggccccg cccgagacaa gatccacgac cttttcatgg agcacgtgat
     1201 gaaacaggcc ccgggctaca acaagctgat ggcttggtgc caaggccctg atcacgaaag
     1261 cgttcccatc atgcccaccc ctgcggcggt tggcagcatc atgcaggcta tcgccaaaga
     1321 ggaaaacatt gaggtggtgg gtgttgatat cggcggtgcc accacggacg tcttttccgt
     1381 cttcacggag gattttatct tcaaccgcac cgtgagcgcc aacctcggca tgagctacag
     1441 catatcaaac gttctggctt ccgcaggctt ggacaacatc atgcgctggg ttcctctgga
     1501 catagatgag aactatctgc gcaacatgat caagaacaag atgatccggc ccaccacgat
     1561 tccctccctg ctggaggaac tcgttttgga acaggccatc gcgatcgaag ctctgcgcct
     1621 ggctttcgag cagcacaaag aatttgccag cagcctcaaa ggcatgcagc gccagcgcga
     1681 catctccgaa gccttcagcc agtcaacttc cggcgccaca atcgtgaaca tgatgaccct
     1741 tgacctcctc gtgggcagcg gtggtgtgct ttcccacgct ccgcgcagac atcagaccgt
     1801 gatgctgctc atcaacgctt tcctgccgga gggcgtaacc cgcctggcgg tggacagcat
     1861 cttcatgatg ccgcatctgg gcgtgctctc cgagatcagc accaaagcgg ccaccgaggt
     1921 gttccgcaag gattgcatgg tctatctggg ctcctgcgta gccccggtgg gcaagagcaa
     1981 ggtggggaaa cccgctctcc acgccagact cgagcttccc gacggcaaga catgggttga
     2041 ggatattcct ttcggcgaag tcagattgat cccctgcggc gtgggcgaag tggccaaagc
     2101 cacactgaga acccacggcg gcctcgtcct cgacgagcac aaaagcaagg aactcacaat
     2161 cgatctgcac ggcggcatcg tcggcatcgt tctggacacc cgcggacgcc agcctttcgt
     2221 gattcccacc gaccgcgccg aacgcatcgc caccctgaag aagtggatga ccgatttcaa
     2281 ggcctatcct gataagatac tgaagtagga ggaaagaatg ggacaagcat actctgccgg
     2341 actcacagta actgacaaca taattctgag aaaagaacgt atcctgcccc tgaagggcca
     2401 ggtgcttgtg aaaaagggcg atcacgtaaa agccgagacc gtggtggcgg aaaccctgct
     2461 tcctggcaag gtggtgcctt tcaacctggc caacaaactt ggcgtcacgc cagcccaact
     2521 gatgaatttc atcaagatcg aagccgggca gcaaatcgac accaacaccg ttttggcaga
     2581 aaccaaaggc ctctttggca ccgggctgtt caaaaacgat gtccgctcac ccgtgaaggg
     2641 agaggtggaa agcatctcct cggtcaccgg acaggtcttg cttcgagaac cgcgcatccc
     2701 cgtgcaagtg aaagccttca tcgacggcgt ggtaaccgac gtgatgccag aggaaggcgt
     2761 catcatcgaa aacaagagcg cctacatcca gggcatcttt ggaatcggcg gcgaaaccat
     2821 cggcgagatc atggtcattt ccaaatcccc ggatgccgag gtcacaccgg ctgaaatcac
     2881 cgaagcctgc gccggcaaga tcatcgtcac cggctccttc gtgtctttcg atgtgatcga
     2941 agccgcccgc aaacacggag ccaaagccat catcacaggc ggaatcgatg accaggacat
     3001 caagaaactc ctgggctacg atatcggcgt ggccatcacc ggacacgaaa acatcggcat
     3061 caccatcgtg gtcacggaag gctttggcaa gataaccatg gcccgcaaga cctttgaact
     3121 gctgaacaga ttcaacggca aaaagacctc catccacggc cgcacccaga tccgcgccgg
     3181 cgttatccgc cccgagataa tcgtgccgct gcccttcgac gaaactgaac tggtggtgaa
     3241 agaaagctcc atgccagtgc tggagccggg aacgctcatc cgcgtgatcc gccaaccgaa
     3301 ttttggcaag atcgcaaagg tcaccggcct gccggaagag cttacaaaag tggaatccga
     3361 gactctcgtc cgtgtgctgg aagcccagtt cgaggacgga agcaagatga tcctgccccg
     3421 cgccaacgtg gaagtgatcg aaacctgaac cctgaattaa ccttcaatgt gatacaggcg
     3481 gtcttcggac cgcctttttt cgaagctgga aaaccccgcc gcattgtctg ggaagaccgt
     3541 tgcacgcaaa agtggacata aagtagtcgt aataacttct tgacaaa
//
LOCUS       DAOOWR010000173         3559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26343_length_3559_cov_3.263413, whole genome shotgun sequence.
ACCESSION   DAOOWR010000173 DAOOWR010000000
VERSION     DAOOWR010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26343_length_3559_cov_3.263413"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLB85_04945"
     CDS             complement(<1..306)
                     /locus_tag="PLB85_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOU72711.1"
                     /translation="MNQKPRFETSLVHGGQKPDPLKAVVTPIYQTSTFAFENAQNGAD
                     CFAGESDGYIYTRLGNPTVRALEDAVAILEGGFGGIALASGMGAVTTIYMSFLSAGAH
                     "
     gene            complement(331..3204)
                     /locus_tag="PLB85_04950"
     CDS             complement(331..3204)
                     /locus_tag="PLB85_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72712.1"
                     /translation="MNKIIKLGLLLLLAAALLLTACSGGDTRAAQIEEFLALDMPHDD
                     GSGVLLKWKPLGGEHRIIQYNIYRGVSPDSLFLLSTQEADPKAGASGNWLNFEDKSFN
                     PLIEFETAPGRLKKEKQQVAEGTLYKGVPRDAKILGEMLKHYDVIGDIKASKYYNSSK
                     RVEIGQGDSKEIYAGYRLNQFNNLYANPKAEHSYYYCVVPVTETGRFLPATPVRKVVP
                     VNNRPDSTAVLHTSYLTDRNELRFEWSPPLGSNNIVAWEAWLMPRSLLPQFREDQIAN
                     AAAPDSVFIANWQRGSILVHQMEPDYWSQSFYDKVDLTEAGTNIPSPEELVNWVPVLT
                     YANYWSSEDGLRQESFQAASLGEKLQIKSSADLPKLPDYQVVEKVYDKGDNIIFSFGR
                     PFAFVTQASWLNKARTRLRVNYEIAENGHQKVSRLKLRFLDADGKVLNEVRESYPDKI
                     IRVKFPKGRNPEIGFNVEVFTEIQGEKNFPGPPISQQVNYDPQAFRFNGGKVTSAGQE
                     LNHLYYDIFSKNNLSGSYSPGMRIGALSRFYEHLVPYVDTQNPLISKVDPKTGLVLTD
                     PHFTVAVDPASGVALSPSLYKKDTEDYLKDLAASIAKLKQTVAPGDSLSEKAQELQAS
                     EAELAYIKGHPAYLAGVKAGSDKAWRRVMLNELDRNSRAYQYKMLATNGKGLWADETD
                     QPELKPTGPGIETPLHPKGEWFDKTKLATLIASIILGLMVVYAIFQARRKDLYIRPIA
                     GLEELDNAVGRATEMGRPVMFVPGWGTLGDPCTISAMMILNKIGGKTAEYDIRLISPQ
                     VDYLVLPLAQEMVLTAYNEAGRPDAYNQDDIFFVSDTQFAFCAAVNGITVREKVATIF
                     YMGYFYAEALLMTETGNQSGAIQIAGSDAITQIPFFITTCDYTLIGEEFYAASAYLSR
                     NLELVAMLKAQDYFKILIVISVLIGTVISTLGINAFLNFLPVE"
     gene            complement(3303..>3559)
                     /locus_tag="PLB85_04955"
     CDS             complement(3303..>3559)
                     /locus_tag="PLB85_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72713.1"
                     /translation="MVPIENVNLALIFIGTIAVLLYFFFSKAHTGVYGKFVSIGKWFM
                     MVGFGASFGLTVMARISLLIGRIQFLVNDVFMAIKQAIAG"
BASE COUNT          721 a          924 c         1087 g          827 t
ORIGIN      
        1 gtgcgccccg gcggagagga aagacatata gatagttgtg accgccccca ttccgctggc
       61 cagggcgatt cctccgaaac ctccttccag gatggccacg gcgtcttcca gagcccgcac
      121 ggtcggattt cccagacggg tgtagatgta gccgtcactt tcgccggcaa agcaatcggc
      181 gccattctgg gcgttttcga aagcgaaggt cgaggtttgg tatatcggcg tcaccaccgc
      241 tttcagggga tcaggtttct ggccgccatg gaccagggag gtttcgaatc ggggtttttg
      301 gttcatcata attccttact atgcttgagc ttattccacc ggcaggaaat tgaggaaggc
      361 gttgatgccc aaggtggaga tgacggtgcc gatcagcaca ctgatgacga tgaggatctt
      421 gaaatagtcc tgggctttga gcatggccac cagttccagg tttcgggaaa ggtaggccga
      481 ggcggcataa aactcttcac cgatgagggt ataatcgcag gtggtgatga agaacgggat
      541 ttgggtgatg gcgtcggaac cggcaatttg gatggcgccg ctctggttgc cggtttctgt
      601 catcagcagg gcttccgcgt agaaatagcc catgtagaag atggtggcaa ccttttcgcg
      661 gaccgtgatg ccgttcacgg ccgcgcagaa agcgaactgg gtgtctgaaa cgaagaagat
      721 gtcgtcctgg ttgtaggcgt cgggccggcc ggcttcgttg taggcggtca gcaccatttc
      781 ctgggccagc gggagcacca gatagtccac ctggggtgag atgagccgga tgtcatattc
      841 cgcggttttg ccgccgatct tgttgaggat catcatggcc gagatggtgc agggatcgcc
      901 cagagtgccc cagccgggca caaacatcac gggacggccc atttcggtgg cgcggcccac
      961 ggcgttgtcc agctcttcca aacctgcgat gggacggatg tagaggtcct tgcgcctggc
     1021 ctggaagatg gcgtaaacca ccatcaggcc cagaatgatg gaggcgatca gggtggccag
     1081 tttggttttg tcgaaccact cccctttcgg gtgcagcgga gtttcgatcc ccggtccggt
     1141 gggcttgagt tcaggctggt cggtctcatc ggcccagagg cctttgccgt tggtggccag
     1201 catcttatat tggtaggcgc gggaattgcg gtccagctca ttcagcataa cgcggcgcca
     1261 ggctttgtcg ctgccggctt taacgccggc gagataggcc ggatgtcctt tgatgtaagc
     1321 cagttcagcc tcgctggcct gaagttcctg cgccttttcc gaaagcgaat cgcccggggc
     1381 cacggtctgt ttcagcttgg cgatgctggc ggccaggtct ttcagataat cctccgtgtc
     1441 ctttttatag aggctggggg acaaagccac gccggaagcg gggtccacgg ccacagtgaa
     1501 atgcgggtcc gtgagcacca ggccggtttt ggggtcaacc ttgctgatca gcggattctg
     1561 ggtatccacg tagggcacca gatgctcata aaaacggctc agggcaccga tacgcatgcc
     1621 agggctgtat gatccgctca ggttgttttt gctgaaaatg tcgtagtaaa ggtggttcaa
     1681 ttcctgcccg gcactggtga ccttgcctcc gttgaaacgg aaggcctggg gatcgtagtt
     1741 cacctgctgg ctgatgggcg ggccggggaa attcttttcg ccctgaattt cggtgaaaac
     1801 ctccacattg aagccaatct cgggattcct gcccttgggg aatttcacgc ggatgatctt
     1861 gtccggatag ctttctctca cttcattcaa aaccttgcca tcggcatcta ggaagcggag
     1921 cttgagccgg gaaacctttt gatggccatt ttcggcgatt tcatagttca cccgcagcct
     1981 ggttctggcc ttgttcagcc aggaagcctg ggtaacgaag gcgaaggggc ggccgaagga
     2041 aaagatgatg ttgtcgccct tgtcataaac tttttccacg acctggtaat caggcagctt
     2101 gggcaggtcc gccgagcttt ttatctgcag cttttcaccc agggacgcgg cctggaagga
     2161 ttcctggcgg aggccgtcct ccgacgacca gtaattggca taggtgagaa ccggcaccca
     2221 gttgaccagt tcctccgggg aaggaatgtt tgtcccggcc tcggtgaggt ccactttgtc
     2281 gtaaaagctt tgggaccaat agtcaggctc catctggtgc acgaggatcg agcctctttg
     2341 ccagttggcg atgaacacgc tgtccggagc ggcggcgttg gctatttggt cctcgcggaa
     2401 ctggggcagc aggctgcggg gcatcagcca ggcttcccag gccacgatgt tgttgctacc
     2461 caaaggcggt gaccactcga agcgaagttc gttcctatca gtcagatagg aggtgtgcaa
     2521 caccgcggtg gaatcgggac ggttgttcac cggcaccact ttccgcaccg gggtggcagg
     2581 caggaacctg cccgtttcgg taacgggaac cacgcagtag taatagctgt gttcagcctt
     2641 gggattggca tagaggttgt tgaactggtt aagccggtag ccggcgtaga tttctttgga
     2701 atcaccctgg ccaatttcca cgcgtttgga gctgttgtag tatttggaag ctttgatatc
     2761 gccgatgaca tcgtagtgtt tgagcatttc gcccaggatc ttggcatccc ggggcacacc
     2821 tttgtaaagc gtaccttcgg caacctgctg cttttccttt ttcagcctgc caggggctgt
     2881 ctcaaactcg atgagggggt tgaagctttt gtcctcgaaa ttgagccaat tgcctgacgc
     2941 accggctttg gggtcagctt cctgggtgga tagaagaaaa aggctgtccg ggctgacgcc
     3001 gcggtagatg ttgtattgga tgatcctgtg ttcgccgcca aggggcttcc atttcagtaa
     3061 aacgccggag ccgtcgtcgt gcggcatgtc gagggcgaga aactcttcta tctgtgctgc
     3121 cctggtgtca ccgcccgagc aggcggtgag gagcaacgct gccgccaaaa gcagcagcag
     3181 tccaagcttt atgattttat tcatgcgttc ttcccatggg ccttgcccac cggttcaagt
     3241 taaaactaac aaggagggga agcccggtta agcttccccc atgtttataa acgctgatcc
     3301 ggtcagccgg cgatggcctg tttgatggcc atgaagacgt catttaccag gaactggata
     3361 cgtccgatca gaagcgaaat acgagccatc acagtgaggc cgaaggaagc gccgaagccc
     3421 accatcatga accatttgcc gatgctcacg aattttccgt aaaccccggt gtgggctttg
     3481 gaaaagaaga aatagagcag cacggcaatc gttccgatga agatcagggc caggttcaca
     3541 ttctcgatcg gcaccatgg
//
LOCUS       DAOOWR010000174         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26515_length_3541_cov_4.450660, whole genome shotgun sequence.
ACCESSION   DAOOWR010000174 DAOOWR010000000
VERSION     DAOOWR010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26515_length_3541_cov_4.450660"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..905)
                     /locus_tag="PLB85_04960"
     CDS             complement(12..905)
                     /locus_tag="PLB85_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOU72714.1"
                     /translation="MEKELQSYLEFLRAEGKSACTVTAYGSDLRQFLGYVTRFFPGEP
                     VAAELSVQMIRDWLRELHDSRVGNRSLARKAAALKSFFLWLKLTGKISENPMNKVKRP
                     KFEKALPHFFTEEEILTLLRIPDLDSVYGVRNRAILELLYSSGLRISELAELRLVDID
                     LKRQLLRVFGKGSKERLVPFGSEAAEAIRAWLPLREKLLRPESSDRIFLTKSGKDFDG
                     RQLYAILGRYFRLVAQKKGYSPHTLRHSFATHLLNRGADLRAVQEMLGHANLETTATY
                     THVTLDDVKKAYKKGHPRGKE"
     gene            986..1741
                     /locus_tag="PLB85_04965"
     CDS             986..1741
                     /locus_tag="PLB85_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOU72715.1"
                     /translation="MNQHIASSAKIGANTVLGVNVVILDDVRIGENCLIGHNVVIHAG
                     CEVGNNVRVDDNTIIGKAPLSSPRSIFKLPEGLKPTVIGDGCQIGANVVIYMQCKIGP
                     RNLIADLATLRENVVIGELNIIGRGVSIENFVTIGSRNKFETNCYITAYSQIDDYCFV
                     APCVATSNDNYMARDPERFKHFKGVTLERGARIGVNATILPGKVLAEDACVAGGAVIS
                     RDVPAGMIVLGSPAKPLREVPEEQLLKNNLDKS"
     gene            1738..2454
                     /locus_tag="PLB85_04970"
     CDS             1738..2454
                     /locus_tag="PLB85_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOU72716.1"
                     /translation="MKALLIIPTYNEIDNIAKIIPAALKQSPDLHILVMDDNSPDGTA
                     AAVKGIMATEPRVHLIERTHKMGLGSAYVTGFKYALKEGYDYVLEMDADFSHNPDDLP
                     RLLEAAAKKDLVIGSRYCHGVNIINWPFKRLLISYFASKYVRLITRMPVKDPTGGFKC
                     FNRRVLEAIDLDSILSDGYAFQIEMNFRAWVKGFRIQEIPIIFTERVDGVSKMSRNIV
                     WEAAWMVWRLHFLRLLGKLR"
     gene            2464..3480
                     /gene="ruvB"
                     /locus_tag="PLB85_04975"
     CDS             2464..3480
                     /gene="ruvB"
                     /locus_tag="PLB85_04975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOU72717.1"
                     /translation="MLERINTPLERPEDTEFDRALRPRTLNEFIGQNQLKQLLDISIK
                     AARLRKEPLDHVLFYGPPGLGKTTLASIIARELGVEITVSSGPVMEKPGDLAGILTNL
                     QRHEVLFIDEIHRLSHVIEEYIYPAMEDFQMEIILDSGPSARTWRISVEPFTLIGATT
                     RAGLLTPPLRDRFGIVLRLDYYDFDSISRIIQRSARILEIPADPEGVAELARRSRGTP
                     RIANRLLRRVRDFAQIMGDGVITLDIALKALEMLQVDHAGLDEMDKRLLFTIMENYRG
                     GPVGLKTLATAVGEDAGTIEEIFEPYLVQQGFLERTPQGRKATFKAYKHLGVKPSDYQ
                     PEIF"
BASE COUNT          768 a         1045 c         1011 g          717 t
ORIGIN      
        1 agggttgccg atcactcttt tccccggggg tggccttttt tgtaggcttt tttcacatcg
       61 tcgagggtga cgtgggtgta ggtggcggtg gtttcaaggt tggcgtggcc gagcatttcc
      121 tgcacggcgc ggaggtctgc tccgcggttg agcaaatggg tggcaaagct gtggcgcaga
      181 gtgtggggcg agtatccctt tttttgtgcc acgaggcgga aataccggcc cagaatggca
      241 tagagctggc gtccgtcgaa atcttttccg cttttggtga ggaaaatcct gtctgagctt
      301 tccggccgga gcagtttttc ccgcaggggc agccaggccc ggatggcctc cgcggcttcg
      361 gagccaaagg gcacgaggcg ttccttgctt cctttgccga aaactctcag cagctgtcgt
      421 ttaaggtcga tatcgaccag gcggagctct gccagttccg agatacgcag tccgctggag
      481 tagagcagtt ccaggatggc gcggttgcga acgccgtaaa cgctgtccag atcggggatg
      541 cggagcaggg tcagaatttc ctcctcggtg aagaaatggg gcagggcttt ttcaaatttg
      601 gggcgcttga ccttgttcat gggattttcc gagattttgc cggtgagttt cagccagagg
      661 aagaaacttt tcagggctgc ggctttgcgg gccagactgc ggtttccaac ccggctgtca
      721 tgcagctcgc gcagccagtc gcggatcatc tgcacgctca gttccgcggc caccggctcg
      781 ccggggaaaa acctcgtcac gtagcccaga aactggcgca ggtcgctgcc gtaagctgtg
      841 accgtgcagg cggattttcc ctccgcccgc aggaattcca ggtagctttg tagttctttt
      901 tccattattt aaataccatg cagatcattt tttgcttgcc tgataacttg tcgagaaaaa
      961 aatagcactt agccaaggag gtttgatgaa tcagcatatc gccagcagcg caaaaatagg
     1021 cgccaacacg gttttgggcg tgaacgtggt cattttggat gacgtccgga tcggggaaaa
     1081 ctgcctgatc gggcacaacg tggtgatcca cgccggctgt gaggtcggaa acaatgtgcg
     1141 cgtcgatgac aacaccatca tcggcaaagc accgctttcc tcgccgcgca gcatcttcaa
     1201 gctgcccgag ggcctcaagc ccacagtgat cggcgacggc tgccagatcg gggccaacgt
     1261 cgtgatctat atgcagtgca agatcggccc gcgcaacctg atcgcggacc tggccaccct
     1321 gcgcgaaaac gtggtgatag gagaactgaa catcatcggc cgcggcgtgt ccatcgaaaa
     1381 cttcgtcacc atcggcagcc gcaacaaatt tgagaccaac tgctacatca cggcctattc
     1441 acagatagat gattactgct tcgtggcgcc ctgcgtggcc accagcaacg acaactatat
     1501 ggcccgcgat ccggagcggt tcaaacattt caagggcgta acgcttgaac gcggtgcccg
     1561 aatcggcgtt aacgccacca tcctgcccgg aaaagtgctg gctgaagatg cctgtgtggc
     1621 cggcggggcg gtgatttcgc gcgatgtgcc cgctggcatg atcgtgctgg gcagtccggc
     1681 caaacccctg cgtgaagtgc cggaggaaca gcttttaaaa aacaacctgg acaagtcatg
     1741 aaagccttac tgatcattcc cacttacaac gagatcgaca acatcgccaa aatcataccc
     1801 gcggcgctga aacaatcgcc agacctgcac atcctggtga tggacgacaa ttccccggac
     1861 ggaaccgccg ccgccgtaaa aggaataatg gccaccgaac cgcgggtcca cctgatcgaa
     1921 cggacccaca aaatggggct tggctctgcc tatgtcaccg gcttcaagta tgccctcaaa
     1981 gaaggctacg actacgtttt ggagatggat gccgattttt cccacaaccc ggacgacctg
     2041 ccccgtctgc tggaagcggc cgccaaaaag gacctggtga tcggctcgcg ctactgtcat
     2101 ggcgtgaata tcatcaactg gcctttcaag cgcttgctga tcagctattt cgcctccaaa
     2161 tacgtgcggc tcatcaccag gatgccggta aaggacccca cgggcggctt caagtgcttc
     2221 aaccgcagag tgttggaagc catcgacctg gacagtatcc tctccgacgg ctatgccttc
     2281 cagatcgaaa tgaacttccg cgcctgggtt aagggcttcc gcatccagga gataccgatc
     2341 atcttcacag aacgcgtgga cggagtatcc aaaatgagcc gcaacatcgt ttgggaggcc
     2401 gcctggatgg tttggaggct gcatttcctg cgcctactgg gtaagcttcg ctagggaaag
     2461 tccatgctgg aaaggataaa cacgcctctg gaacgcccgg aggacacaga gtttgaccgc
     2521 gcgttgcgcc cgcgcaccct caacgaattt atcggccaaa accaactcaa acagcttctg
     2581 gacatctcca tcaaagctgc caggctgcgc aaggaaccac tggaccacgt gcttttctac
     2641 ggcccgcccg gcctgggcaa aaccactctg gccagcatca tcgcgcgcga actgggggtg
     2701 gaaatcaccg tcagcagcgg cccggtgatg gaaaaaccgg gcgacctggc cggcatcctt
     2761 accaacctcc agcgccacga agtgcttttc atcgacgaaa tccaccgcct cagccacgtg
     2821 atcgaggaat acatctaccc cgccatggag gatttccaga tggaaatcat cctggacagc
     2881 ggccccagcg cccgcacctg gcgcatcagc gtggaaccct tcacccttat cggcgccacc
     2941 acccgcgccg gactgctcac cccgcctctg cgcgaccgct ttggcatcgt gctgaggctg
     3001 gattattacg attttgactc catctccagg atcatccagc gctccgcccg catcctggag
     3061 ataccggccg atcctgaagg cgtggctgaa ctggcgcgcc gcagccgcgg cacgccgcgc
     3121 atcgccaacc gtctgctgcg tcgagtgagg gatttcgccc agatcatggg cgatggcgtg
     3181 atcactctgg acatagcgct gaaagccctg gagatgctgc aggttgacca cgccggcctg
     3241 gacgagatgg acaagcgcct gctcttcacc atcatggaga attaccgcgg cggacctgtc
     3301 ggcctcaaaa ccctggccac agcggtgggt gaggatgccg gcaccatcga ggagatattt
     3361 gagccctacc tggtgcagca gggctttctg gaacgcacgc cccaaggccg caaggccacc
     3421 ttcaaggctt ataagcacct gggtgtgaaa ccctctgatt accagccgga gatcttctga
     3481 accttgcggg atgccttaag ctgagaccgg cgcagtccgc agacctctga aaccatggga
     3541 t
//
LOCUS       DAOOWR010000175         3526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26664_length_3526_cov_4.149525, whole genome shotgun sequence.
ACCESSION   DAOOWR010000175 DAOOWR010000000
VERSION     DAOOWR010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26664_length_3526_cov_4.149525"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /gene="leuS"
                     /locus_tag="PLB85_04980"
     CDS             complement(<1..1448)
                     /gene="leuS"
                     /locus_tag="PLB85_04980"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOU72718.1"
                     /translation="MEYPFAKIEPKWQKIWRDKRIFNPGEVAGKPKYYVLAMFPYPSG
                     VLHCGHFSNYSISDAIARLKMMEGFSVMQPMGYDSFGMPAENFAIQHNSHPRITTEEN
                     IAAMRKQFDPMGFGLDWEREVSTCRPDYYVWGQYLFKKLYEKGLVYRKKSWQNWCDNC
                     QTVLANEQVENGLCWRCHGEVDQKELEQWFFRITAYAEELLDFSKLIDWPERVITMQK
                     NWIGKSAGTLIDFPLEDGSEAISVFTTRPDTVYGVTFMALPPEHPLVQKWLAEEPDNH
                     ALHDFCHKVINADKFQRGADDTTKEGVFSGRCCLNPLNGNKVQIWITNYVLMDYGTGA
                     VMAVPAHDQRDFEFAKKYGIPMLLVIQNPEQSLVLDEMAEAYIEPGILVNSAPFDGRD
                     SEEAKAAISALIEEKGCGKVTHTYRLRDWGISRQRYWGNPIPVIHCPKCGVVLVPDED
                     LPVLLPDNVQVGRTTSNPLLSVPEWFNVACPQ"
     gene            1779..2669
                     /gene="htpX"
                     /locus_tag="PLB85_04985"
     CDS             1779..2669
                     /gene="htpX"
                     /locus_tag="PLB85_04985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOU72719.1"
                     /translation="MKALIKRWGLFLTVNCLVLAVLGVIMHLVGVPSESWIQLLAICA
                     VYGFAGAIISLLSSKALAKSSYKITLLTRGTASGKALYLYDTIEKMAAHQGIKMPEVG
                     IYQSADNNAFATGASKNKSLIAFSSGIMQNLDEEELAAVAGHEMTHITEGDMVTTTLL
                     MGTMNTFVLFLANVIASILSARRGQRDENRGSAGVMNNFGQSAIAIILQNVLMVLANI
                     VLAAHSRRREYVADAGSARLTSPGHMITALEKISNAHVRTVRKDAFAIAKINSKSVMS
                     LFATHPPIEKRIAALREMMV"
     gene            2666..>3526
                     /locus_tag="PLB85_04990"
     CDS             2666..>3526
                     /locus_tag="PLB85_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOU72720.1"
                     /translation="MKKNFKLTITKLALEGYGIGFQEGKAVFVPYTAAGDEIEAELLR
                     ERKDVAFARVKEYLRRGEGVIPSSCSSFAADLPCGGCDWLCIDYPTQLKYKTDLLREL
                     LQPLAPELEIPATTPSPEIRHYRNKAFMPVGAGEKGLSFGIYARWSHQIVPHEACQLH
                     PAIFDTIARRCLQIMDQTGVKPYNEMNHTGTLRHIGFRCSRDRKKILLVLVTRSGKLP
                     FTQLLVKQLTAEFPAISGIVQNINRERGNVILGPEEKLLFGEPWLQENLAGLRFRVHY
                     RSFWQVNTGTL"
BASE COUNT          765 a         1023 c          938 g          800 t
ORIGIN      
        1 cattgggggc aggctacatt gaaccattcc ggcactgaaa gcagcggatt gctggttgtg
       61 cggccaacct ggacgttgtc aggcagcagc acaggcagat cttcgtccgg aaccagcacc
      121 acaccgcatt tcgggcaatg gatcaccggg atggggttgc cccagtagcg ctggcgggaa
      181 attccccagt cgcgcagacg gtaggtgtgg gtcaccttgc cgcagccttt ttcctctata
      241 agagcggaaa tggcggcttt ggcttcctcg ctgtcccggc cgtcgaaggg cgcggaattc
      301 accaggattc ccggctcaat gtaagcttcg gccatctcat ccagcaccag gctttgctcc
      361 gggttttgga tcaccagcag catggggatg ccgtattttt tggcaaactc aaagtcgcgc
      421 tggtcgtggg ctggcacagc catgacggcg ccggtgccgt aatccattag cacatagttt
      481 gttatccaga tctgaacctt gtttccgttg agagggttaa gacagcatct gccgctgaac
      541 acaccctctt tggtggtgtc atcggcgcca cgctggaatt tatcggcgtt gataacctta
      601 tggcaaaaat catgcagggc gtggttgtca ggctcttccg ccagccattt ctgcaccagc
      661 ggatgttccg gtggcagggc catgaaagtc actccataaa cggtgtccgg gcgcgttgtg
      721 aacacgctga tggcctcgga gccatcttcc agcggaaaat cgatcagggt gccggcgctt
      781 ttgcctatcc agttcttttg catggtgata acgcgttccg gccagtctat aagcttggaa
      841 aaatcgagca gttcctcggc ataagcggtg atgcggaaaa accactgttc cagttccttt
      901 tggtccactt cgccgtggca gcgccagcag aggccgtttt ccacctgttc gttggccagc
      961 acggtctggc agttgtcgca ccagttctgc catgattttt tgcggtaaac cagtcccttt
     1021 tcataaagct tcttgaagag atactggccc caaacatagt aatcgggacg gcaggtgcta
     1081 acctcgcgtt cccagtccag gccaaaaccc atgggatcaa actgcttgcg catggcagcg
     1141 atgttttcct ctgtggtgat gcggggatgg gaattgtgct ggatggcaaa gttttcagcg
     1201 ggcatgccga aagaatcata acccatgggc tgcatcacgc tgaagccctc catcatcttc
     1261 aggcgggcga tggcgtcgct gatggaatag tttgagaagt gcccgcaatg cagcaccccg
     1321 gaaggatagg gaaacattgc cagcacgtaa tatttgggct tgccggcaac ctcgccgggg
     1381 ttgaagatcc gcttgtcccg ccagatcttt tgccacttag gctctatctt cgcaaagggg
     1441 tattccatca tgtctccttc cggcccggca ttaatgatgc tttgggcctt gttacagcat
     1501 ttgttatgct gatttttgca agggcgggta tttgtcaagc acaagctgac tcaatcccca
     1561 tcccgcgaga ttattcaagc ctcatcccgt cagggctttt ccgttccggt aagagtctga
     1621 tgccgaatcc tcttttatat ggcgaagatt ttccttgcca tttttacgcc tttcagaatt
     1681 gtgaaataac ggaaatttgt ttccctgccg tctgcaggcg gggtattgat ttacaaagta
     1741 aaatgcttat acaatattta ggaggagata actcactcat gaaagcattg atcaaacgct
     1801 ggggactctt cctcaccgtc aactgcctgg tcctggcagt gctgggcgta atcatgcatc
     1861 tggtaggcgt tcccagtgaa agctggattc aactgctggc catctgcgcc gtgtatggct
     1921 tcgccggagc catcatctcc ctgctttcca gcaaggcttt ggccaaatcg tcatacaaga
     1981 tcacgctgct cacccgcgga accgcttctg gcaaagccct ttatctctat gacaccatcg
     2041 agaaaatggc cgcccaccaa ggcatcaaga tgccggaagt cggcatctac cagtctgccg
     2101 acaacaacgc tttcgccacc ggcgcttcca agaacaaatc gctcatcgcc ttctccagcg
     2161 gcatcatgca aaacctggac gaggaagagc tggccgccgt ggccgggcat gaaatgaccc
     2221 acatcaccga aggcgacatg gttaccacca ccctgctgat gggcaccatg aacacctttg
     2281 tgctgttttt ggcaaacgtc atcgcctcca tcctcagcgc ccgccggggc caaagagacg
     2341 aaaacagggg cagcgccggg gtcatgaaca acttcggcca gagcgccatc gccattatcc
     2401 tgcaaaacgt gctgatggtc ctggccaaca tcgttttggc tgcccattcc cgccgccgtg
     2461 agtacgttgc agatgcaggc tccgccaggc tgacgagccc gggacacatg atcaccgccc
     2521 tggaaaagat cagcaacgcc cacgttcgca ccgtgcgcaa ggacgctttt gccattgcca
     2581 agatcaacag caagagcgtc atgtcgctct ttgccaccca ccctccgatc gaaaagcgca
     2641 tcgcggctct ccgcgaaatg atggtttgaa aaagaacttc aagctcacga tcaccaagct
     2701 ggccctggaa ggctacggga tcggatttca agaaggcaag gccgtttttg tgccatacac
     2761 tgctgccggc gacgagatcg aagccgaatt gctgcgggag cgcaaagatg tggctttcgc
     2821 ccgggtgaaa gaatacctgc gccggggaga gggagtgatt ccctcgtcct gttcctcttt
     2881 cgcggcggat ttgccctgcg gcggctgcga ctggctctgc atcgactatc ccacccaact
     2941 gaaatacaaa accgacctgc tccgcgaact gctgcagccc ctggcgccgg aactggaaat
     3001 ccccgcgacc acgccctctc cggaaatccg gcattaccgc aacaaagcct tcatgcctgt
     3061 gggggctggc gaaaagggcc tcagcttcgg tatttatgcc cgctggtccc atcaaatcgt
     3121 gcctcacgag gcttgccagc tgcaccctgc catctttgac accatcgccc ggcgctgcct
     3181 gcaaatcatg gaccagaccg gcgtgaaacc ctacaatgag atgaaccaca ccggcaccct
     3241 gaggcatatc ggtttccgct gttcaaggga ccgtaaaaag atcctgctgg tgctggtgac
     3301 ccgttccggc aaacttccct ttacccaact tctggtgaag cagcttacag ccgaatttcc
     3361 agccatttcc ggcatcgtgc agaacatcaa ccgcgagcgt ggaaacgtaa ttctcggtcc
     3421 cgaggaaaag ctgctttttg gcgagccctg gctgcaggaa aatctggccg ggctgcgctt
     3481 ccgggtgcat tatcgctctt tctggcaggt gaatacaggc accctg
//
LOCUS       DAOOWR010000176         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26672_length_3525_cov_3.508646, whole genome shotgun sequence.
ACCESSION   DAOOWR010000176 DAOOWR010000000
VERSION     DAOOWR010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26672_length_3525_cov_3.508646"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLB85_04995"
     CDS             complement(<1..623)
                     /locus_tag="PLB85_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOU72721.1"
                     /translation="MQYSQKVLDHFMRPRNVGKMEDPDAQATEGSPACGDQVTVYLKI
                     DEESKTIEDISFLSYGCASNIATASIITELAKGKTLEEAKKLSWRDAMEALDGLPPVK
                     VHCSVLAADTLQTAISNYEIAHGLKEVPNFGKDTIVEELKKIIYPQVGEDIVSLKMVK
                     YVGFDNGDVLIDMNMMSFDEWRENVAEEIREHLNKYTEVKNIQINFP"
     gene            complement(624..1817)
                     /locus_tag="PLB85_05000"
     CDS             complement(624..1817)
                     /locus_tag="PLB85_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOU72722.1"
                     /translation="MNPGKIYLDNCVTTPLAPEVLEAMRPYFSERFWYPSPFVSTGEA
                     TSSALSGWHEAIAKTLNATGPEIHFTSGGTMANNIAIKGLAWAHSSAGKHAIVSVVDY
                     PDLLTNAAWLEKQGFEVTYLEPDSEGLVSADKLKAAIRPDTILFLSTIVNHVVGTIQP
                     IEAYGRILAEARHRIFWHADAGQAYGKMPLDVRALGVDTLSISAHKIHGPQGIGALYV
                     KSGIKLAQVIHGVNRLDPLQTGGLSLALIAGFVKAAELTFADLDATTNKLRELSDHLL
                     RRLETTIPEIELNGPRGEGRACHNINVSIAYIEGEAITMMLDMQGITVATGSACASQG
                     LKASYVLMAMGRNHVQSHGSMKFTLSRYNTKEEIDFAVERLAEITASLRQRSPLYRST
                     HPEEK"
     gene            complement(1814..2221)
                     /locus_tag="PLB85_05005"
     CDS             complement(1814..2221)
                     /locus_tag="PLB85_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU72723.1"
                     /translation="MAVSTRTEYALRALLEIPAKSSVSANLICERQQLPKKYVEHLLR
                     ALKKAGLIASSHGSRGGYTLARPAGQISLYDIMKAVKDRNLELDCGMGKQFCLGDDCT
                     LQPLFSDLAARQRDLFRKYSLSRISKIFKQEKE"
     gene            2353..>2808
                     /locus_tag="PLB85_05010"
     CDS             2353..>2808
                     /locus_tag="PLB85_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72724.1"
                     /translation="MIEVTKLGCAYDDKMVLRELDLSLAGDEFTVLVGPNGAGKSTLV
                     YALLGFLPALEGEIRLQGKPLNAYKRSELARLIAFVPQESVFQFDYPVRDIVLMGRYP
                     YLGLMQSWLPADLEAVEEVLEQLQLSDLADRFYSQLSGGEKQRVLIARAL"
     assembly_gap    2809..2818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2819..3144
                     /locus_tag="PLB85_05015"
     CDS             <2819..3144
                     /locus_tag="PLB85_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72725.1"
                     /translation="LAQNTPYIFLDETLSQLDINHQVEIMALLQRIVRDNGKGILLIS
                     HNLNLGANFADRMVFLARGKVLGSGTPEEMMRPELLRELFGMELQTMLNPASGKPNLL
                     YPNSL"
     gene            3189..>3525
                     /locus_tag="PLB85_05020"
     CDS             3189..>3525
                     /locus_tag="PLB85_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72726.1"
                     /translation="MNSAVIEGQVVNPSGKGISSVLISDGLIQIYSSENGMFRISTKA
                     DSLSFNRLGYQSRILAVAKVGKRVTLKPEPVVLPKIIVSESAWDIFSPPVDRVALPVD
                     PDRHYYLAGD"
BASE COUNT          704 a          911 c         1024 g          876 t
ORIGIN      
        1 caggggaagt ttatctggat gttcttaact tccgtatact tattcagatg ctcgcgaatc
       61 tcctcggcaa cgttttcgcg ccattcgtcg aaactcatca tgttcatgtc gatgagcaca
      121 tcgccgttgt cgaagccgac gtatttgacc attttgaggg aaactatgtc ctctcccacc
      181 tggggataga tgatcttttt gagctcttcg acgatggtgt ctttgccaaa gttgggaact
      241 tccttgaggc cgtgggcgat ttcgtagttc gagatggcgg tttgcaatgt gtcggctgcc
      301 agcacggaac aatggacctt caccggcggc aggccgtcca aggcttccat ggcgtcgcgc
      361 cagcttaatt ttttcgcttc ttcgagggtt ttgcctttgg cgagttctgt gatgatggac
      421 gcggtggcaa tgttggaggc gcagccgtag gaaaggaaac taatgtcttc tatcgttttg
      481 gattcttcat ctatcttcag ataaaccgta acctggtcgc cgcaagccgg gctgccctca
      541 gtggcctggg cgtccgggtc ttccatttta ccaacgttgc gggggcgcat gaagtgatcc
      601 agaaccttct gtgaatattg catttatttc tcctcgggat gggttgagcg gtagaggggg
      661 ctgcgctggc gcagcgaggc cgtgatttcg gcaaggcgtt ccaccgcgaa atcgatttcc
      721 tctttggtgt tgtatctgga tagggtgaat ttcattgaac catgtgactg cacgtggttt
      781 ctgcccattg ccataagcac atagctggct ttcaggccct gggacgcgca ggctgaacct
      841 gtggcaaccg tgatgccctg catgtccagc atcatggtga tggcctcgcc ctcgatgtag
      901 gcaatggaga cattgatgtt gtggcaggcg cgcccttcgc cgcgggggcc gttcagctct
      961 atctccggga tggtggtttc caaacgccgc aacagatggt cagaaagctc tctgagtttg
     1021 tttgtggtgg cgtccagatc ggcgaaagta agttcggcgg ctttcacaaa cccggcgatc
     1081 aaagccaggc tcaggcctcc ggtctgtaga ggatcgaggc ggttcacgcc gtggatgacc
     1141 tgggccagtt tgatgccgct tttcacgtag agggcgccaa tgccctgcgg accgtggatt
     1201 ttgtgcgctg aaatgctgag ggtgtccacg cccagggctc taacgtccag aggcatcttg
     1261 ccataggcct gtccggcatc ggcatgccag aatatccggt gcctggcttc cgccagtatc
     1321 ctgccgtagg cttcgatggg ctggatggtg cccacaacgt ggttcacaat ggtgctcaaa
     1381 aagaggatgg tatccggacg gatggccgct ttcagtttgt ccgcgctgac caggccttcg
     1441 gaatccggct ccagataggt cacttcgaag ccctgttttt ccagccaggc ggcgttggtg
     1501 agcaggtccg gataatccac cacggaaaca atggcgtgtt tgccggcaga gctgtgagcc
     1561 caggccaggc ctttgatggc aatgttgttg gccatggttc cgccagaggt gaaatgaatc
     1621 tcaggaccgg tggcgttcag ggttttggca atcgcttcat gccagcccga aagcgccgag
     1681 ctggtggcct cgccggtgct gacaaatggg gatggatacc agaatcgctc actgaagtaa
     1741 ggacgcatgg cttccaaaac ttccggagcc agtggtgtgg ttacgcaatt atcaagatat
     1801 atctttccgg gattcattct ttctcctgtt tgaagatttt ggagatccgg gacaggctgt
     1861 atttcctgaa caggtcgcgc tgcctggctg ccagatcgct gaacagcggc tgcagggtgc
     1921 agtcatcccc caaacagaac tgcttgccca taccgcagtc cagttccaga ttgcggtctt
     1981 tcacggcttt catgatgtcg taaaggctta tctggcctgc cggtcgggcc agggtgtagc
     2041 cgccgcgcga tccatggctg ctggcgatca gtccagcctt tttgagcgct ctaagcaaat
     2101 gctccacata ctttttgggc aactgctggc gttcgcaaat cagattcgcg gaaacgctgg
     2161 atttggcagg tatctccaac agggccctca gggcatattc tgtccttgtg ctaacggcca
     2221 tttttttctc cttggcatgt tcttctgaac atatagtaat ttgctaggct atccaaggca
     2281 aggaaaatcc ccgggctttt ctccgccatt gacagaatta gcctccggca gaaactggct
     2341 ttggaagatg atatgatcga agtaacaaag cttggctgcg cctacgatga caagatggtg
     2401 ctcagggaac tggacctgag cctggcgggc gatgagttca cggttttggt ggggccaaac
     2461 ggtgcgggga aatccacctt ggtttacgcg ctactgggtt ttcttccggc gctggaaggc
     2521 gaaatacgcc tccagggcaa gcctctcaat gcttacaagc gttccgagct ggcccgcctg
     2581 atcgctttcg tgccgcagga aagcgtgttt cagttcgatt atccggtgcg cgacatcgtg
     2641 ctgatgggac gctatcccta tctgggcctt atgcagagct ggcttccggc tgatctggag
     2701 gctgtggagg aggtgctgga gcaattgcaa ctgagtgatt tggccgacag attctattcc
     2761 cagctcagcg gcggcgaaaa acagcgggtg ctgatcgccc gggcgctgnn nnnnnnnnca
     2821 ctggcacaga atacgcctta catttttctg gacgagactc tttcccaact ggacatcaac
     2881 caccaggtgg agatcatggc cttgctgcag cggattgttc gcgacaacgg aaaaggcatt
     2941 ttgctcatct cccacaacct caatctggga gccaattttg ccgaccggat ggtctttctg
     3001 gcccggggga aagtgctggg cagcggaacc ccggaggaaa tgatgcgccc ggagctgctg
     3061 cgagagctgt ttggcatgga actgcagacc atgctcaacc cggccagcgg caaacccaac
     3121 ctcttatatc ctaactcatt atgaaaagaa ttctatttcc tctcctttta cttgtgatgg
     3181 tgacaggttt gaactcggct gtgatcgagg gtcaagtcgt caacccttcc gggaaaggca
     3241 tttcctcggt gcttatatca gacggcctga tacagattta cagctccgaa aatggaatgt
     3301 tcagaatctc cacaaaggcg gattccctgt cttttaaccg tttgggctac cagtcccgga
     3361 tcctggcggt ggcgaaagtg ggaaagcggg tcacgttgaa gccggagccg gtggtgttgc
     3421 ccaagataat cgtcagcgaa agcgcctggg acatcttctc gccgccggtg gacagggtgg
     3481 ctttgcccgt ggaccctgac cggcattatt acctggctgg ggata
//
LOCUS       DAOOWR010000177         3509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26837_length_3509_cov_2.911407, whole genome shotgun sequence.
ACCESSION   DAOOWR010000177 DAOOWR010000000
VERSION     DAOOWR010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26837_length_3509_cov_2.911407"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1611
                     /gene="dacB"
                     /locus_tag="PLB85_05025"
     CDS             118..1611
                     /gene="dacB"
                     /locus_tag="PLB85_05025"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOU72727.1"
                     /translation="MTKFSISRLMHFRLRKGYSVRLLLLVFACFSLAGVPLWGQVLDT
                     QGLKSALDGLMDSSLVNGASVAVAFYAPEDSSLIYGYGAGKELMPASLQKLYTGIGAL
                     ETYGPGHRFQTEIGIRGEVRDGVLEGDLVIKGGGDPTWMEAFYPEGPHRVFEMWADSL
                     KARDIRRVRGNLVGDISLYPTYPYNPSWEAHNLPYVFSPGVGALSFNANMVRFVLKGS
                     SQAGEKAKAYPRHGYNYFELDNQITTVAEKGSAGIWLQVSEDNSRVTLKGKLGVDTPE
                     YLTAAVRNPPLFTLRMLRNTLSEKGLSISGKTVLEEDPAIHDSLETLFVFDSFPLSDI
                     LGVMLKTSSNMIAENLLCNIGAGVENGAVRVEELLAAKGIPGEGFNVIDGSGLARQNS
                     CSASHLGLTLSHAYHQDWFDIFLNSLALPGEDGTLRSRFTELKGKGRLYGKTGYLRDV
                     SNLAGYLRAQDGELYAFVIICNNVSNVANAKKWQASVCDTLLRYSGD"
     gene            complement(1985..>3509)
                     /locus_tag="PLB85_05030"
     CDS             complement(1985..>3509)
                     /locus_tag="PLB85_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOU72728.1"
                     /translation="ADNIVLGNRRKYCGSINTAIAKIFDITVAAAGISKKALEQEGIY
                     HYTSIVHSSSHAGYYPDALPLTVKIAFSSEDGRLYGAQVVGFDGVDKRIEMLAHIIKH
                     KETVHDLVELEHAYAPPFSSSKDPVNLAGMVAENILTGVCKVIHWDELEQLDPANSVL
                     IDARTAMEFGLGSIKGAINLPVDELRGRLAEVPRGKKIVVFCGTGHRSYFACRILTQN
                     GFDEVYNLSGGYTTYEQAHKPQTNEDIFSSDYIDKDDQMYKKEDTSTAGGRTVEADAC
                     GLQCPGPIVRLKIEMDKLLEGDKLRISATDPGFVKDVPAWCKMTGNTLIGIEEIGKKY
                     VATVLKGGGEEVGASGGGRNKTIIVFSDALDRALASFVIANGAATTGKKVTMFFTFWG
                     LNVIKRHHKPKIQKDLMGKMFGMMLAGSSRSLGLSKINFGGMGPVLMRKRMKEKNVDQ
                     LETMILQAQKAGVEMIACQMSMDIMGVSPEELMDGITIGGVATYLSEAEQANINLFI"
BASE COUNT          729 a          946 c         1018 g          816 t
ORIGIN      
        1 cgatccgatc aaggccattt actatttctc gtaaaccaat ttgccccgat gcggggtgtg
       61 gacctggcag ccgggatttg gcttgacaca ctgttaaaga tttacactat tgcgaccatg
      121 actaagtttt cgatttcccg gttgatgcac ttccggctgc ggaagggtta cagcgtcaga
      181 ctgttgctgt tggtttttgc ctgtttcagc ctggctggtg ttccgctgtg gggacaggtt
      241 ctggatactc agggcctgaa atccgctctg gacgggctta tggattcgtc gctggtgaat
      301 ggcgccagcg tcgcggtggc tttctacgct ccggaggaca gcagcctgat ttacggatac
      361 ggggcgggca aggaactcat gcctgcctct ctgcaaaagc tttatacagg catcggcgcg
      421 ctggagacct acggccccgg acaccgcttc cagacagaga tcggcatccg cggcgaagtc
      481 cgcgacggcg ttttggaggg ggatttggtt atcaagggcg gcggagaccc cacctggatg
      541 gaggctttct atccagaggg gccgcaccgg gtgttcgaaa tgtgggctga ttctttgaaa
      601 gcgagggaca tcaggcgggt cagaggcaac ttagtcggag atataagcct atatcccaca
      661 tatccttaca acccaagctg ggaagcccat aacctgcctt acgttttttc acctggagtt
      721 ggtgccctct ctttcaacgc caatatggtg cgcttcgtcc tcaaaggctc ttcccaggcg
      781 ggggaaaaag ccaaggcata tccccgccac ggctacaact attttgagct cgacaaccag
      841 ataaccacgg tcgcggagaa gggctccgcc ggcatctggc tgcaagtttc cgaagataac
      901 agcagagtta ctttgaaggg aaaactaggt gtggacaccc cggaatacct caccgctgca
      961 gtgcgtaatc cgcctctttt cacgctgcgg atgctgcgga acaccctctc ggagaaagga
     1021 ttatccattt ccgggaaaac cgtgctggaa gaagaccccg ccatccacga cagcctcgaa
     1081 actctgttcg tgttcgattc ttttccgcta agcgacatcc tgggggtaat gcttaaaacc
     1141 agctccaaca tgattgccga aaacctgctc tgcaacatcg gcgcaggcgt ggaaaacggc
     1201 gcggtccggg ttgaggaact cctcgcggcg aaaggcatcc cgggcgaggg tttcaatgtc
     1261 atagacggct ccggtctggc ccgccaaaat agttgttccg cctcgcatct cggcctgacc
     1321 ctcagccacg cttaccatca ggattggttc gacatttttc tcaacagtct ggctttaccc
     1381 ggtgaggacg gaactttgag aagcaggttc accgaactca aaggcaaggg tcgcctctac
     1441 ggaaaaaccg gctatctgag ggacgtgagc aacctggctg gatacctacg cgcccaggat
     1501 ggcgaactct acgccttcgt tatcatctgc aacaatgtga gcaacgtcgc caacgccaaa
     1561 aaatggcagg ccagcgtctg cgacaccctg ctgcgttatt ccggagatta aactccggtt
     1621 accaaaactt ttcaaatcag ggcatagcgg agggaaagtg tctcagagac acagacttta
     1681 tctacaaagg cagggcgaag atagctggtt tttctgaaaa aaagctggta actcactaca
     1741 cgccaaccta ttcgtccttt gccctatccc actagcttct aacttacatc ccgttgactt
     1801 cccgtttgac gagtgggaac ttagcgggaa gtgaatgaga tgtgtatggg atggggtaaa
     1861 gcacggatca agtcacgaaa accgggccgg acagggaatt ctgcccagat cctgtgagtc
     1921 gggtttgaaa gagagcaaaa aaagcccgga tcgcgccggg ctttataatc ggatggggtc
     1981 tatctcagat gaagaggttg atgttggcct gctcggcttc ggagaggtaa gtcgccactc
     2041 cgccgatggt tatgccatcc atcagctctt ccgggctcac gcccatgata tccatggaca
     2101 tctggcaggc gatcatttcc acgccggctt tctgggcctg gaggatcatc gtttcaagct
     2161 ggtccacgtt cttttctttc atgcgcttgc gcatcaggac gggacccatg ccgccgaaat
     2221 tgatcttgga aagccccagc gaacggctgc tgccggccag catcatgccg aacatcttgc
     2281 ccatcaagtc tttctggatt ttgggcttgt ggtgcctctt gatcacattc agtccccaga
     2341 aggtgaagaa catggtcacc tttttgccgg tggtggcggc gccgttggcg atcacgaagg
     2401 aggccagggc gcggtccaga gcgtcgctga agacaatgat ggtcttgttg cggccgccgc
     2461 ccgaggcgcc aacttcctcg ccgccgccct tgagaacggt ggccacgtat tttttgccga
     2521 tttcctcgat gccgatcagg gtgtttccgg tcattttgca ccaggcggga acgtctttca
     2581 cgaagcccgg gtcggtggct gagatacgca gtttgtcgcc ttcgagcaat ttgtccatct
     2641 ctatcttgag gcgcacaatg ggcccggggc actgcagccc gcaggcgtcg gcttccacgg
     2701 tgcgtcctcc ggctgtggaa gtatcttctt ttttatacat ttggtcgtcc ttgtcgatgt
     2761 agtcgctgga aaagatgtcc tcgttggttt ggggtttgtg ggcctgctcg taggtggtgt
     2821 agccaccgga gaggttgtaa acttcgtcaa agccgttctg ggtaaggatg cggcaggcga
     2881 aatagctgcg atggccggtg ccgcagaaga caacgatctt ctttccccgg ggaacctctg
     2941 ccaatctgcc gcgcagctcg tccacaggca gattgatggc tcctttgatg ctgcccaggc
     3001 caaattccat ggcagtgcgc gcgtcgatca ggacggagtt ggcggggtcc agttgctcca
     3061 attcgtccca gtggattacc ttgcaaactc cggtgaggat gttttccgcc accatgccgg
     3121 ccaggttcac gggatccttg gaactggaaa atggtggcgc gtaggcgtgt tcgagctcca
     3181 ccaggtcgtg cacggtctct ttgtgcttga tgatgtgggc cagcatctcg atgcgtttgt
     3241 ccacaccgtc gaaacccacc acctgagcgc cgtagaggcg tccgtcctcg ctggagaaag
     3301 ctatcttgac ggtgaggggc agggcgtccg gatagtagcc cgcgtgtgag gaggaatgga
     3361 cgatggaggt ataatggtag atgccttcct gctccagagc ctttttggag attcccgccg
     3421 cggccaccgt gatgtcgaag atcttggcga tggcggtgtt gatggagccg cagtatttgc
     3481 gccggttgcc cagaacgatg ttgtcggcg
//
LOCUS       DAOOWR010000178         3500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_26925_length_3500_cov_2.846154, whole genome shotgun sequence.
ACCESSION   DAOOWR010000178 DAOOWR010000000
VERSION     DAOOWR010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26925_length_3500_cov_2.846154"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PLB85_05035"
     CDS             <1..1431
                     /locus_tag="PLB85_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72729.1"
                     /translation="KGYLLLQCNLPGVKYQINGREYTEVQYLPLTPGMYQVRAFGANP
                     VTGPFAEERQMEIRQGETTVGKFDFPHGTLSLFSNETYARFTIDNAKLGKVENLPLPP
                     GTYTVTATLPKAVTGFGDYLITRPITIQAGMHLDQSMDFRYGTVTINSSLDDTRYLID
                     GVSKKKVQKLKLLEGEHSYTAFPPAPHTARSGTFTIRAGEDLPLELEFAKTRQISDAE
                     KRREYTWRFGLMPAAVLEANHYFSFAGAGQSGYTPLCNGFSVSGLRFKAINLWQKELT
                     QPYTSRYPNYMIGGSLLDQAVFTWEKDNKHSGILFDLASVSTGLAHTSPAGFLHAQIE
                     LIGHFSYQKPLVSEIGYYSYVTAFKEGSGRTYRVWDFQWGGETRLQLGVRLAKYNYLN
                     LHLGARYQQPLGGDWYSNNDIAAWMTEDAELPQPDWPAGVPARNAAFDGFSLYAGIGL
                     ETNLIPSLLGLLLDKADKTEGSDLLN"
     gene            complement(1829..2416)
                     /locus_tag="PLB85_05040"
     CDS             complement(1829..2416)
                     /locus_tag="PLB85_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72730.1"
                     /translation="MPRGGIALARFLAGRFAVSEPKAHPAFGTAKLTLEGIQLEFVMT
                     RSEIYRSGNRFPQVRFAPLSADCLRRDFTVNALYQRISDADILDPCTLGQQDLHNRLI
                     RCVRNPHTSFKEDPLRLLRALRFAAVLDFELEAQTLAAIRDLAPLVRQLSQARCQSEK
                     ERLNSVASPQQLDRWQDLAKSTGIGDHLPNTIAST"
     gene            complement(2559..>3500)
                     /locus_tag="PLB85_05045"
     CDS             complement(2559..>3500)
                     /locus_tag="PLB85_05045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HOU72731.1"
                     /translation="GGGTPSLLEADQIKSLCGLFDLAQEAEVTLEINPIQITAPYLQK
                     LGETPVNRLSIGLQSMDDKDLAWLGRRHRSDRIPAQIRLCREHGLDNLSLDLIYGLPG
                     QDVDSIRRNLDYYLALSPDHISAYLLTPDPDTPLGRDLTSGAQTPLPDEDDLAAQYDT
                     LRETLIAAGFEHYEISNFCRPGMASRHNLTYWKSKPWLAVGASASGWLPPLRYTNPAD
                     LDQYYHNIDTQKMPEEAGECSLEQACADHIMMGLRLLEGLDLNELQSLYQYDLRSAKA
                     AEIAKLSASGLLALEGDRLRLTDTALFVSNAVIGELL"
BASE COUNT          795 a          988 c          984 g          733 t
ORIGIN      
        1 aaaggttatc tgcttctgca atgtaacctt cccggtgtga aataccagat caacggccgt
       61 gaatatactg aggtgcagta tttgcctctg accccaggaa tgtatcaggt ccgcgctttc
      121 ggagcaaatc cagtcacagg cccctttgcc gaggaacgcc agatggaaat ccgacaaggc
      181 gaaaccaccg tcgggaaatt tgattttccc cacggtaccc tgagcctctt cagcaacgaa
      241 acctatgccc gtttcaccat cgacaacgcc aaactcggca aagtggaaaa cctgcccctc
      301 ccgcccggaa cctacacagt gacagccact ttgcccaagg ccgtcaccgg gtttggggat
      361 tacctgatca cccgtccgat aaccatccag gccggcatgc acctcgacca gagtatggat
      421 tttagatacg gcaccgttac catcaactct tctctggatg acacacgcta cctgatcgac
      481 ggcgtctcga agaagaaggt gcagaaactc aaacttttgg aaggtgagca cagctacaca
      541 gcctttccac ccgcgcccca caccgcgcga tcgggaactt tcaccatccg tgccggggaa
      601 gacctgcccc ttgaattgga attcgccaaa acccgccaga tcagcgacgc ggaaaaacgc
      661 cgcgaataca cctggcgctt tggcctgatg cctgccgcgg tgctggaagc gaaccactat
      721 ttcagcttcg ccggcgccgg ccaatcaggt tatactcctt tatgcaacgg attctctgtc
      781 agcgggttgc gcttcaaggc catcaacctc tggcaaaaag aactcaccca accctacacc
      841 tcacgctatc cgaactacat gatcggcggc agcctgctcg accaggcggt tttcacctgg
      901 gaaaaagaca acaagcattc cggcattttg ttcgacctcg ccagcgtcag cacaggattg
      961 gcacatactt ctcccgccgg ttttctgcac gcgcagattg agctgatcgg ccatttttcc
     1021 tatcaaaagc cgctggtgag cgaaatcggc tattactcct atgtgactgc cttcaaagaa
     1081 ggtagcggac gcacatacag agtctgggac ttccagtggg gcggcgaaac caggctgcaa
     1141 ctgggcgtcc gcctggccaa gtacaactac ctgaatctac acctcggtgc ccgttaccag
     1201 caacccctgg gtggggactg gtattcaaac aacgatattg ccgcctggat gaccgaagac
     1261 gcggaattgc cccaacctga ctggccagcc ggcgttcccg cccgcaacgc cgctttcgat
     1321 ggattcagcc tctacgcagg catcggcctg gaaaccaatc tcattccctc cctgctgggt
     1381 ttgctgctcg acaaagcgga taaaaccgaa ggaagcgacc tgctgaactg aatcccgggc
     1441 tcttatcccg ttcatcccgc tttacccggt ccagacatgc tccctgaagg ttttggctca
     1501 agctcaatta ggatgaataa agtccgtttt gaaaaagagc catcaatgct aatccatcgc
     1561 aatgtcctgc cagtctggcc ctgagcgctc gaggtcatct ccctcatacg cctctcgtat
     1621 aatcctcgca tcaaatacgg gcatgatgcg ggaggcgcgt ccgggacgca ggaaaaggcc
     1681 ttaatggcca gacggtattg cttctctcgt tcgtggggaa caagagttgg aatgacggca
     1741 gtcgaaccct ctttctataa aaatgagagt aagataggtc ttggatgatg aatgccggtc
     1801 gggatgggga ttataggcag atgctatttc aggtgcttgc gatagtattg ggcagatggt
     1861 cgccgatgcc tgtggacttg gccagatcct gccaacggtc cagttgttgg ggcgaagcaa
     1921 ctgaattgag ccgctccttt tcgctttggc agcgagcctg gctcagttgg cggaccagcg
     1981 gagccagatc gcggatggcg gcaagggttt gggcctccag ttcgaaatcc agcacggccg
     2041 cgaaacgcag ggcacgcagc aggcgcaggg gatcttcctt aaacgatgtg tggggatttc
     2101 tgacgcagcg gatgaggcgg ttgtggaggt cttgctggcc aagggtgcag gggtccagaa
     2161 tatccgcgtc cgaaatcctc tgatagagcg cgttcacagt gaaatcgcgg cggaggcagt
     2221 cagcactgag aggggcgaaa cgaacctggg gaaagcggtt tccagaacgg tagatttcgc
     2281 tccgggtcat cacgaattcc agctggatgc cttccagagt gagtttggcg gttccgaagg
     2341 caggatgggc cttgggttca gagacagcga accgacctgc cagaaagcgg gccagggcga
     2401 tgccgccacg gggcaattca cggcaatgtc ggcgtccaaa acctgatcag acctgccgag
     2461 caattggtcg cgcacgcatc cgccggcaaa gtagcatttt ccccagaatt ttgagtttac
     2521 ggtggcttca gccagaagca accgcagctc ttccagcttc acagcaattc cccaatcacg
     2581 gcattggaaa caaagagagc cgtgtccgtc aggcgcaatc ggtctccctc gagggcgagg
     2641 agtccggaag cgctcagttt ggcgatctcg gccgcttttg cgctacgcag gtcatattgg
     2701 tagaggcttt gaagttcatt caggtccagt ccctcaagca gccttagccc catcatgatg
     2761 tggtcagcgc aagcctgctc cagagagcat tccccggcct cttccggcat cttttgcgtg
     2821 tcaatgttgt gatagtattg gtccaaatca gccggattgg tataacgcaa aggcggcagc
     2881 cagcccgacg ccgaggcgcc cacagctagc caaggcttgc ttttccagta agttagatta
     2941 tgacgcgaag ccattcccgg gcggcagaaa ttggaaatct cgtagtgttc gaaaccggcg
     3001 gcaatcaagg tttcacgcag ggtatcgtat tgcgcggcaa ggtcatcctc atccggaagg
     3061 ggtgtttgcg ccccggaggt aaggtcgcgt cccaaaggcg tgtccggatc gggggtgagc
     3121 agataggcgg aaatatggtc tgggctaagg gcgaggtagt agtcgaggtt gcggcgaata
     3181 ctgtccacgt cttgtccagg cagcccatag atcaggtcca gggagagatt gtctaatcca
     3241 tgttcgcggc aaagcctgat ctgggcggga atccggtctg agcggtggcg ccggcctagc
     3301 caggccaggt ctttgtcatc catgctctgc aagccgatgg aaagccggtt cacgggtgtt
     3361 tcgccaagtt tttgcaggta gggtgcagtg atctggatgg gatttatctc cagggtgacc
     3421 tcggcctcct gagccaagtc gaacaggccg caaagggatt tgatctgatc cgcttccaac
     3481 agcgaaggcg tgccgccacc
//
LOCUS       DAOOWR010000179         3483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_27090_length_3483_cov_3.377771, whole genome shotgun sequence.
ACCESSION   DAOOWR010000179 DAOOWR010000000
VERSION     DAOOWR010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27090_length_3483_cov_3.377771"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLB85_05050"
     CDS             <1..596
                     /locus_tag="PLB85_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72732.1"
                     /translation="MSAKGENPNATLKIVNKNIDFDTVLSYTHGKIYYGGDYELLNMD
                     QPVAYEIVSGGKTYTGNITLDTWPQMVNVPTWPEFHQDTNYSPSWTISADPKFHIIEA
                     GAYGAETVMTVIRQIPGTEKTFTLDQTHWSKIKPIEEFSFGINAVGYEMMNKNKVLIT
                     GISHNYYYWSQYELKTSHQTQRNPLRFMDQIQADMDK"
     gene            669..1661
                     /locus_tag="PLB85_05055"
     CDS             669..1661
                     /locus_tag="PLB85_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HOU72733.1"
                     /translation="MSKKSALALFSGGLDSILAVAWMQKMGHKVHPVCFTAPYITADR
                     AIESARANGMEITVVDISASHLKMLENPIHGFGKNHNPCIDCHGLMFAEAAKLLPRFG
                     ADFLISGEVLGQRPMSQRRNALQAVDKLSGHASLIIRPLSQKLLPDTLPITEGWVDKD
                     QLLDISGRGRSRQIALAKELGLSYPGPGGGCLLTDRSYSLRLRELIKHGQDSEANIRL
                     LRWGRHFRLNPSLKFIVGRDRADNASLEAEDYPGLWFLLRDLEGPLGLLTDPEPSPDL
                     ISLAASILLYYSKKAPSPAWVKYGVDRNRWREICVEKCPESVFRPLLISLDKDL"
     gene            1661..2290
                     /locus_tag="PLB85_05060"
     CDS             1661..2290
                     /locus_tag="PLB85_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU72734.1"
                     /translation="MLKIEDHCLFEEFGTNTWLLWDEDSREAWLIDPAAPSEALLARI
                     RELGLKVTLLVNTHGHGDHIGGNAWFKEALGAPVLIHPADAPLLTDNRRNFSEYLGNP
                     LRLDPPDLLAEDGQILKLGDHSIRVIHTPGHTLGGICLLANGFLLSGDTLFQQSIGRT
                     DLPGGNHAQLITSIREKLFSLPDDTVVFPGHGPRTSIGLEKKSNPFVRP"
     gene            2290..>3483
                     /gene="guaA"
                     /locus_tag="PLB85_05065"
     CDS             2290..>3483
                     /gene="guaA"
                     /locus_tag="PLB85_05065"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU72735.1"
                     /translation="MNRHDTILILDFGSQYTQLIARALRSLSVYCEIHPYNIAPEKIR
                     ELAPKAIILSGSPFSLLDPNSPQPSEMIWSLGLPLLGICYGQQLIADHFGGTIERHTN
                     REYGRAVLKPSLPHPLLEGIPAGAEVWMSHGDSISRLPEDFQALASTATVPYAAIGHL
                     SRPVYALQFHPEVVHTPQGKTLLANFALRIAGCVPDWTPENFISSSQEKIRATVGDAK
                     VVLGLSGGVDSSVAALLIHRAIGDHLIPIFVDNGCMRFQEARRVQEAFSAVPDINIRS
                     VDASELFLTRLAGIEDPEKKRRIIGHTFIEVFEREAAKYPGVAFLAQGTLYPDVIESV
                     SFRGPSATIKSHHNVGGLPDKMGLKLIEPFRELFKDEVRAVGRELGLPETLISRHPFP
                     GPGLAV"
BASE COUNT          760 a         1145 c          850 g          728 t
ORIGIN      
        1 ggatgtcagc caagggcgag aatcccaacg ccaccctcaa gatagtaaac aaaaacattg
       61 acttcgatac agttctttcc tacacccatg gcaaaatcta ttacggtggt gactacgagc
      121 ttcttaatat ggaccagcca gtggcttacg agattgtgag cggcggcaag acctacaccg
      181 gcaacatcac cctggatacc tggccccaaa tggtgaatgt tcccacctgg cctgaatttc
      241 accaggatac gaactactcc ccctcctgga ccatctcagc cgatcccaaa ttccatataa
      301 tcgaagccgg cgcttacggc gcggaaacgg taatgactgt catccgccag attcccggca
      361 cggaaaagac cttcaccctg gatcaaaccc actggagcaa gatcaagccc atcgaagagt
      421 tctctttcgg catcaatgcc gtcggttacg agatgatgaa caagaacaag gtcctcatca
      481 ccggcatttc ccacaactac tattattgga gccagtacga gctcaagacc tcccatcaga
      541 cccaacgcaa ccctctccgc tttatggacc agatccaggc ggacatggac aaataatctc
      601 gctttagagt caaatagtcc tatgggcctg cttttgacgg caggcccttt ttgatggaaa
      661 aggatgccat gagcaaaaaa tctgccctcg ccctcttctc cggaggcctg gacagcatcc
      721 tggccgttgc ctggatgcaa aaaatgggac acaaggtgca tcccgtctgc ttcaccgcac
      781 cctacatcac ggcagacagg gcaatcgaaa gcgcgcgggc caacggcatg gaaatcaccg
      841 ttgtcgacat ttccgcctcc catctgaaga tgctggagaa ccccatccac ggcttcggca
      901 agaaccacaa tccctgcatc gactgccacg gcctgatgtt cgcggaagcg gcgaaactgc
      961 tgccccgctt tggcgcggat ttcctcattt ccggtgaagt gctgggccaa aggcctatgt
     1021 cccagcgccg aaacgccctc caggcagtgg acaaactcag cggccacgct tccctgatta
     1081 tccgtcccct atcccagaaa ttgctgccgg acacccttcc catcactgaa ggctgggtgg
     1141 ataaggacca gttgctcgat atcagcggac gcggacgctc ccgccagatc gctttggcaa
     1201 aggaactcgg cctctcctat cccggccccg gcggtggctg cctgctcacc gaccgaagtt
     1261 attccctccg cctgcgcgaa ctcataaaac acggccagga cagcgaagcc aacatccgcc
     1321 tcctccgctg gggacgccac ttccgcctca atccaagcct caaattcatc gtgggacgtg
     1381 accgggctga taacgccagc ctcgaagcag aggattatcc cgggctctgg ttccttctgc
     1441 gcgatctgga aggacctctg ggcctgctca ccgatccgga accctccccc gacctcatat
     1501 ccctggccgc ctccattctg ctctattaca gcaaaaaagc tccctctccc gcctgggtga
     1561 aatatggtgt tgacaggaat cgttggcgtg agatttgtgt ggaaaagtgt cctgaatctg
     1621 tcttcaggcc gcttctgatc tctctcgaca aggatttgtg atgctcaaga ttgaagacca
     1681 ttgcctgttt gaagaattcg gcaccaacac ctggctgctc tgggacgagg attcccgcga
     1741 ggcctggctg atagaccccg ccgccccttc ggaagctctg ctggcacgga tcagggaact
     1801 gggcctcaag gtcacccttt tggtgaacac ccacggccac ggcgaccata tcggcggaaa
     1861 cgcctggttc aaagaggccc tcggcgcccc ggtgctcatc catccagccg acgcccccct
     1921 gctcactgac aaccgccgca acttcagcga atatctggga aatccactgc gccttgaccc
     1981 gccagacctc ctggctgaag atggccaaat cctgaaactg ggcgatcaca gcattcgggt
     2041 gattcacact cccggccaca ctcttggcgg gatctgcctt ttggcaaatg gattcctcct
     2101 cagcggcgac actcttttcc agcaaagcat tggccgcacc gatctacccg gcggaaacca
     2161 tgcccagctc ataacctcaa tccgcgaaaa gcttttctcc ctgccagacg acacggtggt
     2221 ctttcccggt cacgggccca ggacgtccat cggactggaa aagaagtcca atccatttgt
     2281 gaggccttga tgaaccgcca cgacaccatc ctcatcctcg atttcggttc tcagtacact
     2341 cagctaatcg cccgcgccct acgttccctc agtgtctatt gcgaaatcca tccctataat
     2401 atagcgccgg aaaagattcg cgaactcgcc cccaaggcca tcattctttc cggcagccct
     2461 ttcagtttgc tcgatcccaa ctctccccag ccctctgaaa tgatctggtc gctcggtctg
     2521 cctctactcg gcatctgcta cgggcagcaa ttgatagctg accattttgg cggcaccatc
     2581 gagcgccaca caaaccgaga atacggccgc gcagtgctga aaccttccct cccccatccc
     2641 ctgttggaag ggattcccgc cggcgccgag gtctggatga gccacggaga ttctatttcc
     2701 cggcttccgg aggattttca ggccctcgcc tcaaccgcca ccgtgcccta tgccgccatc
     2761 ggccatctca gccggcctgt ttacgcgctc cagtttcatc ccgaggtggt ccacacgccc
     2821 caggggaaaa cccttttagc caatttcgca ctcaggatcg ctggctgcgt tccggattgg
     2881 acgcccgaaa actttatctc ctccagccag gagaaaatcc gcgccacggt tggcgacgcg
     2941 aaagtggtgc tcggcctttc cggcggggtg gattcctctg tagccgcctt gcttatccac
     3001 cgtgccatcg gagaccacct catacccatt tttgtggata acggctgtat gcgctttcag
     3061 gaagcccggc gcgtgcagga ggccttttct gccgttccag acatcaacat cagaagcgtg
     3121 gacgcctctg aactctttct gacgcgcctc gccgggatcg aggatcccga aaagaagcgc
     3181 cggatcattg gccacacctt catcgaggtt ttcgaacgtg aagccgccaa ataccccggt
     3241 gtggctttcc tggcccaggg cacactctac cctgatgtga tcgaaagtgt ctctttccgc
     3301 gggccttccg ccaccatcaa aagccaccat aacgtcggcg gattgccgga taagatgggc
     3361 ctcaaactga ttgagccttt ccgcgagctt ttcaaagacg aggttcgcgc cgtgggcagg
     3421 gagctcggtc tgccggaaac cctcatcagc cgccatcctt tccccggtcc cggcctggct
     3481 gtg
//
LOCUS       DAOOWR010000180         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_27145_length_3477_cov_3.521333, whole genome shotgun sequence.
ACCESSION   DAOOWR010000180 DAOOWR010000000
VERSION     DAOOWR010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27145_length_3477_cov_3.521333"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3477
                     /locus_tag="PLB85_05070"
     CDS             <1..>3477
                     /locus_tag="PLB85_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU72736.1"
                     /translation="TPTFPGIEYIQPNYLSTFHIEPNDPLYSEQFHTMVANPQAWNYS
                     TGSSQVVVGIIDSGCLINHPDLRANIYINPGEDPALGLHDNGIDDDGNGYIDDWCGWD
                     FSDAPELSDIAVGDYIDQDNDVEDENFHGTHVAGIVGAVGNNGIGISGVCWNVKLMPI
                     RAGFRTTAGQGYLQDDDAAAAIIYAADNGCNVINLSWGDPNYSPIIGDACNYAHSKGV
                     TIVASAGNDPGPVLSYPAKLSNVISVGAVNRNRNIAGFSSYGPDLDLVAPGEMVLSTY
                     KTDLGEEYFLQSGTSMSSPFVAGAAALLLSLHPNLSPDEVRARLLTSTDDLGAPGFDI
                     YYGHGMLNTKKLLENTQPPLVYIDQPLDHTGITGSMDITGTVTGSDFFRYSVMYTNAE
                     VPTILDWYDVQTHQNYPSHHQQQVQNGVLAHFDIPEAFAEGKYTLRVQYESVSGQKFN
                     YFRTFIYDRTPPILREESVMGFKRYDGQNLRYYVSAVFNEYVRTELLITDSAGGLYTV
                     YGALLDSTHVWAIPSQLPEGPIDYQIRATNVCNIEYLSPVWQDALDIRYEIIPSYGYN
                     WQMIGEPRVPLNHFHDYDGDGYKEYLAMDLPKTGYGEVFVYQPQAGGHVLKHSFNDSF
                     WLLSAGNTNDIGQEILMLKGDTAVLMESQLTSTYPNLPLWEETSVTGGVIAKYSRDNV
                     EGLLLVKILPTKRVIQAYKRSGNTFEKKNQLENNSETDFYNTFVPTIIVKNFDNDNYP
                     DILTADTDGDVMIYEIRNDNMHELSWSHRMPVGNTYSITSGDFDGNGRQDFFIGGYYK
                     DNLDPNQNFWYFEGFRNVSNNNYTSMGSIMFNEVTSQSAILAHDLDNDGKDEIILAIA
                     PNLYTLKYDGGKFKPQFYGKSFRNYALMAYKDANNRSYFLTNYEVEPDSVIFVEWTSE
                     DPFTGPGTPANFQAQALDGNTVQLSWIDNGAQFYRLYRRDEDGQTIVLDDVSDVSYMD
                     LQVETGTRYSYAVTACDPAYSPMESIPTVWKDVVPYETPQLKSVTMTGSNTLLISFDQ
                     PMSGQILNPSLYHVSHGMGRPSSVNSTATHYGAQLFFNSQFPAIEEDFTLTLSNIISA
                     QNIPLDQTQFTFSWQQDTVAPGVVEALVLEDQQTVRIQFTESIAPLNPNPEIIGNYIL
                     HNHSNDPDNSIV"
BASE COUNT          888 a         1067 c          806 g          716 t
ORIGIN      
        1 aactccaacc ttcccgggca tcgaatacat acagccgaac tatctttcca ctttccacat
       61 cgaacccaac gatccactgt attcagagca attccacacc atggtggcga atccccaggc
      121 ctggaattac tccaccggca gttcccaagt ggtggtgggg atcatagact ccggttgcct
      181 catcaaccat ccggacctga gagccaacat ctacatcaac cccggcgaag atccagccct
      241 ggggcttcac gacaacggca tcgacgatga cggcaatggc tacatcgacg actggtgcgg
      301 ctgggacttt tccgacgccc cggagctttc agatatcgcc gtgggtgact atattgacca
      361 ggataatgac gtggaggacg aaaacttcca cggaacccac gtggcaggaa tcgtcggcgc
      421 ggtgggcaac aacggcatcg gtataagcgg cgtttgctgg aacgtgaagc tgatgccgat
      481 cagggctgga ttccgcacca ccgccggcca gggttacctg caggatgacg acgcagcagc
      541 cgcaattatt tacgccgccg acaacggctg caacgtgata aacctgagct ggggcgatcc
      601 aaactattca cccatcattg gcgatgcctg caattatgcc cattccaagg gtgtaaccat
      661 cgtggcttcc gctggcaacg atcctgggcc tgtgcttagc tacccggcca aactttcaaa
      721 tgtgatctcc gttggagccg tcaaccgcaa ccgcaatatc gccggcttct ccagctatgg
      781 cccggatctg gacctggtag cccccggtga aatggtgctc agcacttaca aaacggattt
      841 gggtgaagaa tacttcctgc aaagcggcac ctcgatgtcc tcaccctttg tggcaggtgc
      901 cgcggccttg ctgctgtctt tgcacccaaa cctttctccg gatgaggtgc gtgcccggtt
      961 actcacctca accgacgacc ttggcgcacc tggttttgac atctattatg gacacgggat
     1021 gctgaacacc aaaaaactgc tggaaaacac ccaaccacct ttggtttaca tcgaccagcc
     1081 tctagaccac acaggcatca caggcagcat ggacatcacc ggaacggtga ccggtagcga
     1141 tttcttccgc tacagcgtga tgtacaccaa tgccgaggtg cccacaattc tggactggta
     1201 tgatgtgcaa acccaccaaa actatccttc ccaccatcag cagcaggttc aaaatggcgt
     1261 tttggcacat ttcgacatcc ccgaggcctt cgctgaggga aaatacacgc tccgggtgca
     1321 gtatgaaagc gtttcgggcc agaaattcaa ctacttccgc accttcatct acgaccgcac
     1381 gcctcccatc ctgagagaag aatccgtgat gggtttcaag cgctatgacg gccaaaacct
     1441 gcgttattat gtgtctgccg tgttcaatga atatgtgcgc accgaactgc tgataaccga
     1501 ttccgccggc ggactataca ccgtttacgg cgccttgctg gacagcaccc acgtttgggc
     1561 catcccctcc caactgccgg aaggccccat cgactatcag atcagagcca ccaacgtttg
     1621 caacatcgaa tacctttccc ccgtctggca ggatgctctg gacattagat atgaaatcat
     1681 acccagttac ggttacaatt ggcaaatgat cggcgaaccg cgagtcccat tgaatcactt
     1741 tcatgattac gacggagacg gctacaagga ataccttgcc atggacctgc ccaaaacagg
     1801 atacggcgag gtcttcgtct atcagcccca agccgggggg catgtgctca agcactcctt
     1861 caacgattcc ttctggcttc tgagcgcggg caataccaac gacatcggcc aggaaatact
     1921 gatgctcaag ggcgacaccg ctgtgctgat ggaaagccag ctcaccagca catatccaaa
     1981 cctaccgctc tgggaggaaa cctcagtcac aggaggtgtg atcgcaaagt attcacgtga
     2041 caatgtcgaa ggcctcctgc tggtgaaaat acttcccacc aaacgagtta tccaagctta
     2101 caagcgctcc ggaaacacct tcgagaaaaa aaaccaactc gaaaacaatt ccgaaacgga
     2161 tttctacaat accttcgttc ccactatcat tgtaaagaat tttgacaatg acaactatcc
     2221 tgacatcctc acagccgata ccgatggcga cgtgatgatc tacgaaatcc ggaacgacaa
     2281 tatgcacgaa ctatcctgga gccacagaat gccggtcggc aacacctata gcatcaccag
     2341 cggcgacttc gacggcaacg gccgtcagga tttctttatc ggcggatatt acaaggacaa
     2401 tctggacccc aaccagaact tctggtattt tgaaggcttc cgaaacgttt ccaacaacaa
     2461 ctacaccagc atgggctcca tcatgttcaa cgaagttaca tcccaaagcg ccatcctggc
     2521 ccacgatctg gacaacgacg gaaaggacga gatcatcctc gccatcgccc ccaaccttta
     2581 caccctgaag tatgacggag gcaaattcaa accccagttt tacgggaaaa gtttccgcaa
     2641 ctatgcgctc atggcttaca aagatgccaa caaccgctcc tatttcctga ccaattacga
     2701 ggtggaaccg gattccgtta tctttgtgga atggacttcc gaagaccctt tcaccggacc
     2761 cggcacacct gccaatttcc aggcccaggc attggacggc aacactgtcc agctctcctg
     2821 gatagacaac ggggcgcagt tttatcgcct ctaccgcagg gatgaggatg gtcagaccat
     2881 tgttttggac gatgtttcgg atgtttctta catggacctg caagtggaga cggggaccag
     2941 atacagctac gccgtaaccg cctgtgaccc agcctactcg cccatggaga gcatccccac
     3001 cgtctggaag gacgttgtac cctatgaaac gccccagttg aaaagcgtca ccatgaccgg
     3061 ttcgaacacg ctgctgatca gctttgacca gcccatgtcg ggccaaatcc taaaccccag
     3121 cctctaccac gtaagccacg gcatgggccg tccatcctcc gtgaacagca ccgccaccca
     3181 ttatggcgcc cagcttttct tcaacagcca gttcccggcc atcgaagagg atttcaccct
     3241 caccctcagc aacatcatca gcgcccaaaa catcccgctt gaccagaccc aattcacttt
     3301 cagctggcag caggataccg tggcccccgg tgtggtggaa gcccttgttc tggaggacca
     3361 gcagaccgtg cggatacaat tcaccgaatc catcgccccg ctgaacccaa atccggaaat
     3421 catcggcaac tacatccttc acaaccattc caacgacccc gacaactcaa tcgtgag
//
LOCUS       DAOOWR010000181         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_27251_length_3468_cov_3.186053, whole genome shotgun sequence.
ACCESSION   DAOOWR010000181 DAOOWR010000000
VERSION     DAOOWR010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27251_length_3468_cov_3.186053"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..2093)
                     /locus_tag="PLB85_05075"
     CDS             complement(117..2093)
                     /locus_tag="PLB85_05075"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72737.1"
                     /translation="MRRIGLFLLLLGNVCLTAESIQIGNGSLLNQHLPIEPFVSYSYS
                     QQLYRAADIGFTGQVSAVSFQYNIPSTYFIGSSAVWKLYLGHTQREQLDAWVPLDSLV
                     LVFDGTLSEDQFSNGIPGQGWLNINLDTVFHFNGSDNLLIAVDENDPDFGSNADDFIC
                     TETAEVRGIVFASNTLNPDPASPPPGPENLFTRLAYPNLRLEITPFSLTPWHPQPEDQ
                     ATGIPTSTDLRWQSNASSFDVWLGTDPDELQLMAQGIASQQWTPPQPLGLLTSYCWQV
                     VAHADGETYPGPVWSFSTAGEGIGPPQNLSAYHITDHVQLNWQPPLEGEPVMYRVIRN
                     GVFLAATQEIGYQDYEVGPGQVLYYYLLAQNHLGELSDPSNTVTIHIPDIIPNLILQQ
                     SFEACSPFSQNIPGWQNLDMDNSPTWSPWPMQGFGEPLAWLVFPPAQLSPPQTGLEAH
                     SGAAMAASFNVQTPPNNDWLITPRLQLGSAPSLNFWARSHTVDYGLERLRVLISTTDA
                     DPASFTSLNSGNWLSVPAEWTEYSFDLAAWQGQSVHLAFNCVSWDALALYLDDIVVTG
                     EGGYVPVGEDIAVPDCFKVFPNPARGTFSVETTGKSPFNLALYDLRGRELFSIRNLSS
                     FHSAEHALNLAAGVYFLRLEAEGGSQLKRLAVIK"
     gene            2419..>3468
                     /gene="hydF"
                     /locus_tag="PLB85_05080"
     CDS             2419..>3468
                     /gene="hydF"
                     /locus_tag="PLB85_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOU72738.1"
                     /translation="MSSTPRGERLIITLLGNRNSGKSSLINALTNQEIAIVSDTPGTT
                     TDPVDKRYELLPLGPVTFYDTAGIDDTGELGAKRVQATFRVLFRTDIAIFVNNGNPFS
                     ASELEFLQRIREMKVPVLVAFNKSDLRQPDPANLEYCRQHGLEWVSVSASQKENIPEA
                     KEKLIALAPAHLKEDRPLVSDLVRAGERVILVTPIDSAAPKGRLILPQVQVLRDLLDS
                     GTVPVVVREFELKQALAALKEDPDLVITDSQAIELVVRELSPSVKLTTFSILFARYKG
                     DLGILANGIRQIDKLKEGDRILIAEACSHHVQKDDIGRVKLPRWLKEYTKKNLIFETY
                     AGHDFPPNLEEFALCV"
BASE COUNT          808 a          930 c          998 g          732 t
ORIGIN      
        1 agcccagatg gcttccaccg gttccaggga ctggcgggcg acgatatttt ctctgaattt
       61 cttgtcctgg agaagtccct cgaggaaagc ctccacatct ggcagaacat agcttttcat
      121 ttgataacag ccaggcgctt caactggctg ccgccttccg cttccaagcg taggaaatag
      181 acgccggcgg ccaggttcag ggcgtgttcc gcgctgtgga aactactcag attacggatg
      241 gaaaaaagtt cgcggccccg caggtcgtag agggcaaggt taaaaggcga tttgccggta
      301 gtctcaacgc tgaatgttcc gcgggcgggg ttgggaaata ccttgaagca atccgggacc
      361 gcaatatctt cccccactgg aacataacca ccctctccag tgaccacgat atcgtccaga
      421 tagagcgcga gggcgtccca ggaaacgcaa ttgaaggcca gatgaacgct ttggccctgc
      481 caggcggcga gatcgaagct gtattcggtc cattcagccg gcacggacag ccagttgccg
      541 ctattcaagc tggtaaagct ggcaggatcg gcgtccgtgg tggaaatcag cactctcagc
      601 cgctccagcc cataatccac agtgtgggag cgggcccaaa agttcaggct gggagcagaa
      661 cccagttgca gtctgggagt gatcagccaa tcgttgttgg gtggggtttg cacgttgaaa
      721 gacgctgcca tcgccgcgcc gctgtgggct tcgagaccag tttgcggcgg ggatagttgc
      781 gctggaggaa aaaccagcca ggcaagcggc tcaccgaaac cctgcattgg ccagggagac
      841 caggttgggg agttgtccat gtccagattt tgccagccgg gaatgttctg gctgaacggg
      901 gaacaggcct caaaactctg ctgcaggata aggtttggga tgatgtccgg aatgtgtata
      961 gtaacggtgt ttgagggatc agaaagctcg cccagatggt tttgggccag cagataatag
     1021 tataagacct gcccgggccc aacttcataa tcctgatagc caatttcctg tgtggcagcc
     1081 agaaacacgc cgttccgaat aacgcggtac atcaccggtt cgccttcaag tggcggctgc
     1141 cagttcagtt gaacgtggtc tgtgatgtga taggcgctta ggttttgggg cggaccaatg
     1201 ccttcgccag cggtgctgaa gctccagacc ggaccggggt aagtttctcc gtccgcgtga
     1261 gccaccacct gccaacaata agaagtcagc aatcccaaag gctgcggtgg tgtccattgc
     1321 tgtgaggcaa tcccttgcgc cataagctgt aactcgtccg gatcggtgcc caaccaaaca
     1381 tcgaatgacg aggcgttgct ctgccaccgc aaatcggtgg aggtaggaat tccggtggcc
     1441 tggtcttcgg gttggggatg ccagggtgtg aggctgaagg gtgtgatttc cagccggaga
     1501 ttgggatagg ccaggcgcgt gaacaggttt tccggacctg gcggcgggga ggcaggatcg
     1561 ggattcaagg tattgctggc aaatacgata cctcgcactt ccgcggtttc tgtgcagatg
     1621 aaatcgtcag cgttggaacc gaaatcggga tcgttttcat ccacggcgat cagcaggttg
     1681 tcgctgccat tgaagtggaa caccgtgtcc agattgatat tcagccaacc ctggccgggt
     1741 atcccgttgg aaaactgatc ctcggacagg gtgccgtcga acaccaggac cagactgtcc
     1801 agaggcaccc aggcatccag ttgttcgcgt tgggtgtgcc ccagatagag cttccagacc
     1861 gcgctgctgc cgataaaata tgtgctgggg atattgtact ggaaagagac agcgctcacc
     1921 tggccggtga aaccgatatc cgcggcccgg tagagttgtt gagagtagct gtagcttaca
     1981 aaaggctcga tgggcagatg ctggttcagc agcgacccat tgccgatctg gatgctttct
     2041 gctgtcaggc agacgtttcc aagcaacagc agaaacagcc cgattcggcg cattaactcc
     2101 ccttatccca tacaattgga tgtagcctgt tgaggttgtg gaggagctgt tcgcgcagct
     2161 tgtccggatc agcttcccga tagtttatgg tatgaggtcc cttaatgtag tccatgctcg
     2221 gttcctgtaa ctattgtaat gcaaaaaaca agctttccat aagtttgcaa gttgtcaagg
     2281 acgaattgcg cagattcact ttgcttcatg aacactgatg ccaaaccatc actgccaacc
     2341 ggaacagaac ttgccaaagc caggaggaag cgttattctg tttaataaag agaaatatgc
     2401 aaccaagaac gaggaaaaat gagcagcacc ccccgcggcg aacgcctgat catcaccttg
     2461 ctggggaacc gcaacagcgg caagtccagc ctcatcaacg cccttacgaa ccaggaaatt
     2521 gccatcgttt ccgacactcc gggcaccacc accgacccgg tggacaagcg ttacgagctt
     2581 ctgcctctgg gccccgtgac cttttacgac acggctggca tcgacgatac cggagaattg
     2641 ggcgccaagc gtgtccaagc cactttccgt gtgcttttcc gcacggatat cgcgatcttt
     2701 gtaaataacg gcaatccctt ttccgccagc gagttggagt ttctgcaacg cattcgcgaa
     2761 atgaaggtcc cggtattggt ggcattcaat aaaagcgacc tccgccaacc tgaccccgcc
     2821 aatctggaat actgccggca acatggtctg gaatgggttt ccgtctccgc cagccaaaaa
     2881 gaaaacatcc cggaagccaa ggaaaagctg attgccctgg cgccggcgca tttgaaagag
     2941 gaccggcccc tggtgagcga cctggtgcgg gctggcgagc gagtgatttt ggtgacgcca
     3001 atcgactccg ctgccccgaa aggccggctg atcctgcccc aggtgcaggt gctgcgcgac
     3061 cttttagaca gcggcacggt gcctgtggtg gtgagggaat ttgaactgaa gcaggccctg
     3121 gccgccttga aagaggatcc cgacctcgtt atcacagatt cccaggccat cgaactggtg
     3181 gtgcgtgagt tgtcgccaag cgtgaaacta accacctttt caatcctttt tgcccgctac
     3241 aagggcgatt tgggcatcct ggccaacggc atcaggcaaa tcgacaaact taaggaaggc
     3301 gatagaatcc tgatcgcgga agcctgctct caccacgttc agaaggacga cataggccgg
     3361 gtgaagctgc cccgctggct gaaggaatac accaaaaaga acctcatttt cgaaacctac
     3421 gccggacacg atttcccgcc aaacctggag gaattcgcgc tctgtgtg
//
LOCUS       DAOOWR010000182         3369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28288_length_3369_cov_3.529873, whole genome shotgun sequence.
ACCESSION   DAOOWR010000182 DAOOWR010000000
VERSION     DAOOWR010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28288_length_3369_cov_3.529873"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLB85_05085"
     CDS             <1..262
                     /locus_tag="PLB85_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72739.1"
                     /translation="IYRGSHILLRCFRKTTPPDELISLCCSLAAWYSKARFSQNVPVD
                     YTQIRYVRKPRKSPPGFVVYTEHRTVFADPRDLRSVREELKQ"
     gene            259..1011
                     /gene="recO"
                     /locus_tag="PLB85_05090"
     CDS             259..1011
                     /gene="recO"
                     /locus_tag="PLB85_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOU72740.1"
                     /translation="MKTKLKDRAFLIKSIPYSNSSLILKAFGHEHGQLSLLAKGIRSK
                     PQASLLNPLCLYEFSFYEPRESGLCLLAELSLVKEFDFTDRIEVWTAAECALELYSQL
                     IIPAEESVAYFELLRNYLVYLDSLQTNAVLIWWRFLLRVFRMLGIPCDTQACSSCGGK
                     ALKAWDKGSGKLFCDECWNALPDLQNCELLSPVSAMVLSLMPVIGEHLSTLKLDRNSV
                     SSLNELFARYYQAHFNKALRLRGLDVLEQFYT"
     gene            1343..1909
                     /locus_tag="PLB85_05095"
     CDS             1343..1909
                     /locus_tag="PLB85_05095"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOU72741.1"
                     /translation="MKPKLRALILVSVLLLSFSLLGAESFGSRTAASLRARGVSPWLI
                     VVIISMLPVVELRGAIPVAIALFGMSWQEAVLLSILGNMIPIPFLLLFLEWFLKLISR
                     FRWGKKFTDWLYARTRSKGKVVERYAELGLIIFVGIPLPGTGGWTGAVAAKIFGLDFW
                     KSFVCVFLGVLLAAIAITLITLLGTTIF"
     gene            1913..3061
                     /gene="lpxB"
                     /locus_tag="PLB85_05100"
     CDS             1913..3061
                     /gene="lpxB"
                     /locus_tag="PLB85_05100"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU72742.1"
                     /translation="MGSSAFHIFWLAGENSGDLHASLVMKSLNAAIPGLRHSGIGGPR
                     MQREGLKPLFPFSKFNVMGFAEVLGRLGFFWKVERFLDTFFRDDRPDLAILVDYPGFN
                     LRIARLADSHRVPVLYYICPQFWAWKHNRVFQLRDHVRHVACILPFEPELLARHNVAA
                     TYVGHPIAEEISFELDRDSFASLYGLDPAKRWIGFLPGSRDNEISRMLPVYLKAARKL
                     ASQGFEILVSKALTVSRSLFTSLLENTRTAGLKVIDGYRYDLMRHSDLLVCTSGTATL
                     EAAFIGTPMLICYKTSAFSYAIGRRLVQIKRVGLPNILLDQNLLPELIQSACTPENIV
                     RTALSLLSDQDRLHEIKLELLKLQTSLSEPYPSLEMPRLVKDLLDVHV"
     gene            3054..>3369
                     /locus_tag="PLB85_05105"
     CDS             3054..>3369
                     /locus_tag="PLB85_05105"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOU72743.1"
                     /translation="MSELLLRLEGVLGALLLRLWRRTLRVVALNQPPIGHPCVYALQH
                     RDLLLLTLQRIDHDIAVMVSRSRDGELIARPLARLGFFPVRGSTSRSGSQALKVMVRL
                     ARE"
BASE COUNT          679 a          983 c          832 g          875 t
ORIGIN      
        1 gatttaccgc ggttcccaca tcctgcttcg ctgtttccgc aaaaccactc ccccggatga
       61 gcttatcagc ctttgctgtt cactcgcggc ctggtattcc aaagcccgat tttcgcaaaa
      121 cgttcccgtg gattacactc aaatccgcta tgtacgcaag ccccgtaaaa gtccaccagg
      181 attcgtcgtt tatacagagc acaggacggt ttttgccgat ccccgcgatc ttcgttccgt
      241 gcgtgaggaa ctgaagcagt gaaaaccaag ctcaaggacc gcgcttttct gataaaaagc
      301 attccctaca gcaacagcag cctgatcctg aaagcttttg gacacgaaca cggacaactt
      361 tcactcttgg ccaaaggcat ccgctccaaa ccccaagcca gcctcctcaa cccgctctgc
      421 ctctatgaat tcagctttta tgagccgcgc gaatccggtc tttgcctgct tgctgaactt
      481 tcacttgtga aagagtttga cttcacggac cggatcgagg tctggacagc tgctgagtgc
      541 gctttggagt tatattcaca gcttattatc cccgctgagg aaagcgtcgc gtattttgag
      601 ctgctgcgga actaccttgt gtatttggat tcactgcaaa cgaacgccgt gcttatctgg
      661 tggcgttttc tgcttcgggt tttcaggatg ctgggtattc cctgtgatac acaagcctgc
      721 tcctcctgcg gcggcaaggc cctcaaagcc tgggataaag gcagcggcaa actgttctgc
      781 gacgaatgct ggaacgcctt gcccgacctt caaaactgcg agctgctcag ccccgtctca
      841 gctatggttc tgagcctgat gccggtgatc ggggaacacc tgtccaccct gaaactggac
      901 cggaacagcg tgtccagcct caacgaactc tttgcccgtt attaccaggc ccatttcaac
      961 aaagccctca ggctgcgagg gctggacgtt cttgaacaat tctacacctg ataatcaatc
     1021 ccaactgact cgcaagcact acgttaccca ttcgctttgt aagtgattca tttaaaagtg
     1081 agccttactt gtttactcac aatcatatca tgccggtcac acccttagcg ctcgagtcta
     1141 tacctctccc acgcctcccg cactattccc gtatcaaatg cggggataat gcgggaggcg
     1201 cattcgcggc ccatgacaag gccttaacgg cccgacagga tcgtaagctc aatttcaggc
     1261 tgtgcgctgg aatttccctt gtcaaaatct ccacatagcc aaagatggat tctcgctata
     1321 aaatgatgaa taggaagacc gcttgaaacc gaaacttcgc gctttgatcc ttgtgtcagt
     1381 cctgctgctc agcttcagcc tgctgggcgc tgaatccttc ggctcgcgca ccgccgcttc
     1441 attgcgggcc cgtggtgtga gtccctggct aattgtggtg attatctcaa tgttgcccgt
     1501 tgttgaactg cgtggggcca tccctgttgc cattgcgctt tttggcatgt cctggcagga
     1561 ggcagtgttg ctgtcgatat tgggcaacat gattcccatt ccctttctgc tgctctttct
     1621 ggagtggttt ctgaagctga tttcccgctt ccgctggggc aagaagttca ccgactggct
     1681 ctacgcccgc acccgcagca agggcaaggt ggtggaacgc tacgcggagc tcggtctgat
     1741 catatttgtg ggtatccccc tgcccggcac gggaggttgg accggagcgg tggctgccaa
     1801 aatctttgga cttgatttct ggaagtcctt tgtctgtgtc ttcctcggcg tgctgctggc
     1861 tgcaatcgcc attacgctaa tcactttgct gggcaccaca atattctgaa tcatgggctc
     1921 ctccgctttt cacatcttct ggctggctgg tgaaaactcc ggcgacctgc atgccagctt
     1981 ggtgatgaaa agcctcaacg ccgccattcc cggcctgcgc cacagcggca tcggcgggcc
     2041 cagaatgcaa cgcgagggct taaaaccgtt atttcccttc tccaaattca acgtgatggg
     2101 ctttgcggaa gtgcttggcc gtctgggctt tttctggaaa gtggaacgtt ttctggacac
     2161 ttttttcagg gacgatcggc ctgatttggc cattttggtg gattatcccg gcttcaactt
     2221 acgcatcgcc cgtctggcag attcacatcg cgtgcctgtg ctttactata tctgccccca
     2281 gttttgggct tggaaacaca accgcgtttt ccaactgcgt gaccacgttc gccacgtggc
     2341 ctgcatcctg ccttttgagc cggaattgct ggccaggcac aatgttgctg ccacctatgt
     2401 gggccatccc atcgcggagg aaatcagttt tgaactcgac cgtgacagct ttgccagttt
     2461 atatggtctc gatcctgcca aacgctggat cggctttttg cccggcagcc gcgacaatga
     2521 aataagccgg atgctgcctg tctatctgaa agcggcacgg aaactggcat cccaaggttt
     2581 cgaaattctt gtttccaaag ctctcaccgt ttctcgcagt ctcttcacgt cactgcttga
     2641 aaacacccgg accgcgggac tgaaggtgat cgacggctac cgctacgatt tgatgcgcca
     2701 ttctgatttg ctggtctgta cttctggcac agccaccctg gaagctgcct ttatcggcac
     2761 tccaatgctg atctgctaca aaacttccgc cttctcctat gccatcgggc gtcgcctggt
     2821 gcaaatcaag cgcgttggac ttcccaacat cctgctggac caaaacctct tgcctgagct
     2881 gatccaatcc gcctgcacac ccgaaaatat cgtccggaca gcactcagcc tgctgtctga
     2941 ccaagaccgg ttacatgaaa taaagcttga actgctcaag cttcagactt cattgtctga
     3001 accgtatccc agcctggaaa tgccgcgctt ggtgaaagac cttctggacg tccatgtctg
     3061 agctgctatt gaggctggag ggtgttctgg gcgctttgct gctgcgtttg tggcgccgta
     3121 ccctgcgtgt agtagcactg aatcaacctc ccattggcca tccttgcgtt tacgcgcttc
     3181 agcaccgaga tttgctgctg ctcaccttgc agaggataga tcatgatatc gccgtgatgg
     3241 tctctcgctc ccgggatggt gaactcattg ctcgccctct ggcccgcctg ggttttttcc
     3301 cggtgcgcgg ctcaacctcc agaagcggct cccaggcttt gaaagttatg gttagactgg
     3361 cccgtgaac
//
LOCUS       DAOOWR010000183         3364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28342_length_3364_cov_4.061046, whole genome shotgun sequence.
ACCESSION   DAOOWR010000183 DAOOWR010000000
VERSION     DAOOWR010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28342_length_3364_cov_4.061046"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLB85_05110"
     CDS             complement(<1..773)
                     /locus_tag="PLB85_05110"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOU72744.1"
                     /translation="MQDAIIALYKNKPEVYTPADRSLFEDFIQALNEGRIRACEPTEQ
                     GWKVNQWIKMGILIGFRMGELAEIPWSERKSFFDKDTLPEKVFTLKDRVRLVPGGSSA
                     RSGCFVAPGVAVMPPSFINIGAYVDSGTLVDSHALVGSCAQIGRNVHLSAGAMIGGVL
                     EPIGSRPVVIEDDAFIGGNTGIYEGILVQKRAVIASGTIITASTPIWDSVRQEFLQRD
                     PGGSFTVPEGAVVVPGSRQMKNDPSFQVYCPVIVKYRDA"
     gene            complement(777..1943)
                     /locus_tag="PLB85_05115"
     CDS             complement(777..1943)
                     /locus_tag="PLB85_05115"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HOU72745.1"
                     /translation="MSYDFDRITNRRGSGCFKYDGLNMIYGRDDLISLWVADMDFPVA
                     PAIMEALQKRLDHGIFGYNLRLPVFYDTVLNWVEKQYGYKADGDWMLSTPGVMPAVSL
                     AVTVLTEPGDGVLIQTPVYRPFHNAALDQGRVLLTSPLLLRDGRYEIDFDDLESKLKG
                     AKLFILCSPHNPVGRLWSEAELRKMGQLCRQHGVTVISDEIHADLVFDGAKAVSIAAL
                     DDFADITICCMSAAKSFNLAGLATSVVLVKNPSLRQPLASAIEEYHLFMGNSFGIEAT
                     IAAYRDSEVWLQALLTYLEGNRAFLLDAFESELPQLKMLKPEACYLAWIDFRALGLSD
                     KAIADLLVNKARLALDPGLKFGDEGAGFQRLNFGCPRSVLAEAVDRLKKAISEV"
     gene            complement(1940..2479)
                     /locus_tag="PLB85_05120"
     CDS             complement(1940..2479)
                     /locus_tag="PLB85_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72746.1"
                     /translation="MPRELNINSVRGRMPVRYRVPVLSRVLGIVAHILISGYCIFVLF
                     SVIRATSPFLMKVLPLLVLFVSLDALFRHFTTLNSVIFTPECLWFRYLLLPSVPIEYE
                     KISSMELRKVITYYMFLEFTDRRGKTRVLKSPASFPRMMEIMYNIADLAPQARLNDEL
                     DKMVGVIRRMKEKQSEVEQ"
     gene            complement(2464..>3364)
                     /locus_tag="PLB85_05125"
     CDS             complement(2464..>3364)
                     /locus_tag="PLB85_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOU72747.1"
                     /translation="DFWGARNTSIEKEIMIVKNIDPIKLINLHLHTNVSDGLLSPAQL
                     VRRAQQIGLDLISITDHDTADAYRMLPKDIAPLRILPGMEISSQHEGSDVHVLAFGVN
                     LASTSLVEMMEMYLIGRRERAIRMIEKLADLGMEITLEEVVAISGSRELIVRPHIAQI
                     MVDRGYVASKNEAFDKYIGNDRPAFSPKPEFSVPDAIRVIHEADGFAIIAHPGKLEKE
                     SYIEEFIAMGIDGLEVWHPDHYQYQIQAYTEICQNNGLYMTAGSDFHGDPDRHNHFDA
                     VPADSFILESVNRLWREYLCRVS"
BASE COUNT          761 a          947 c          924 g          732 t
ORIGIN      
        1 ttggcgtcgc ggtatttcac gatcacgggg caatagacct gaaagctggg atcattcttc
       61 atttgtcggc ttccgggaac aaccacggcg ccttccggca cggtgaagga gccgccaggg
      121 tcacgctgga gaaactcctg gcgcactgaa tcccagatgg gcgtggaagc cgtgatgatg
      181 gttccggagg caatcacagc ccgcttctgg accaggatgc cttcgtagat gccggtgttt
      241 ccgccgatga aagcatcgtc ttctatcacc acagggcggg aaccgatggg ctcaagcaca
      301 ccaccgatca tggccccggc tgagagatgg acgtttctac cgatctgcgc gcaagacccc
      361 accaaggcgt gggaatcaac cagggtgccg ctgtccacat atgccccgat gttgatgaag
      421 gaaggcggca tcaccgccac tccgggagca acgaagcagc cgctgcgcgc ggaacttcct
      481 cccggcacca ggcgcacccg gtccttgagt gtgaaaacct tctccggcag ggtatccttg
      541 tcaaaaaagc tcttgcgttc gctccagggg atttcagcaa gctcgcccat ccgaaaacca
      601 atcaggatgc ccatcttgat ccactggttc actttccagc cttgttcagt aggctcgcag
      661 gccctgatcc gtccttcgtt cagagcttgg atgaaatcct cgaagaggga cctgtccgcg
      721 ggagtgtaaa cctctggctt gtttttgtat agggcaatga tggcgtcttg catggtttaa
      781 acctcgctga tggctttttt aagccggtcc actgcttcgg ctaggaccga cctggggcaa
      841 ccgaaattca gcctttggaa gcccgcgccc tcgtctccga atttcagccc gggatcgaga
      901 gccagcctcg ctttgttgac cagcaggtcc gctatcgcct tgtctgagag gcccaaagcg
      961 cgaaaatcta tccaagccag atagcatgcc tccggcttga gcatcttcaa ctggggaagc
     1021 tcgctctcga aagcatcaag cagaaaggcc ctgttgcctt ccagataggt cagaagcgct
     1081 tgcagccaga cttccgaatc ccggtaagcg gcgatggtgg cttcgatgcc gaagctgttg
     1141 cccataaaga gatggtattc ctcgatggcg gaagccaaag gctggcgcag agaagggttc
     1201 ttcaccagca ccaccgaggt ggcgagtccg gccaggttga aggatttggc cgctgacata
     1261 cagcagatgg tgatatccgc gaaatcatcc aaagccgcga tggaaacggc ttttgccccg
     1321 tcaaagacca gatcggcatg aatttcgtcg ctgatcacag taacgccgtg ctgacggcaa
     1381 agctggccca ttttccgcag ctccgcctcg ctccagagcc tgcccacagg attgtggggg
     1441 ctgcagagga tgaaaagctt cgcgcctttc agcttgcttt caagatcgtc aaaatcaatc
     1501 tcgtagcgcc catccctcag cagcagcggg gaggtgagca agacccgccc ctgatccaaa
     1561 gccgcgttgt ggaaaggacg gtaaaccggg gtttggatca gaactccgtc gccaggctca
     1621 gtcagcacag tgaccgcaag gcttacagcc ggcatcactc caggcgtgga aagcatccag
     1681 tcgccgtccg ccttgtagcc atactgcttt tccacccagt tcagaaccgt gtcgtaaaac
     1741 acaggcaggc gcagattgta gccaaagatg ccgtgatcga gccgcttttg caaggcttcc
     1801 atgatagctg gcgccacggg aaaatccatg tccgccaccc aaagcgaaat cagatcgtcc
     1861 cgcccataga tcatgttcag gccgtcatac ttgaagcaac cgctgccgcg ccgattggta
     1921 atccggtcaa aatcatagct cattgttcca cctcgctctg cttttccttc attctgcgga
     1981 tcacgcccac catcttgtcc agttcgtcat tcagccttgc ctgcggggcc aggtccgcga
     2041 tgttgtacat gatctccatc atcctgggaa acgaggcggg gcttttcagc acacgcgttt
     2101 ttccccggcg gtcggtgaac tccagaaaca tgtaataggt tatcaccttg cgcagttcca
     2161 tgctgctgat cttttcgtat tcgatgggca cagagggaag caacagatag cggaaccaga
     2221 ggcattccgg ggtgaaaata actgaattca gcgtggtgaa atgcctgaag agagcgtcca
     2281 gcgacacgaa cagcaccagc agcggaagaa ccttcatcag aaaagggctg gtggcgcgga
     2341 tcacggagaa cagcacgaaa atgcagtagc cggagataag gatgtgggca acaatgccca
     2401 aaaccctgct caacacaggc acccgataac ggacgggcat gcgcccccga acggaattga
     2461 tattcagctc acgcggcaaa ggtattccct ccaaaggcgg ttaacgcttt ccaggataaa
     2521 gctgtcggct ggaacggcat cgaagtggtt gtggcggtcg gggtcgccgt ggaaatcgct
     2581 tccggcagtc atgtagaggc cgttgttctg gcaaatctcg gtataggcct ggatttgata
     2641 ctgatagtgg tccggatgcc agacctcaag gccgtcgatg cccatggcga tgaactcctc
     2701 gatgtaactt tccttttcca gcttgcccgg atgcgcgata atcgcgaagc cgtcagcttc
     2761 gtggatgacc cggatcgcgt ctggaacgga gaattccggc ttgggcgaga aggcgggtcg
     2821 atcgttgccg atatatttgt cgaaagcttc attcttgctg gccacataac ctcggtccac
     2881 catgatttgc gcgatatgcg gacgcacgat cagttcacgg cttccggata tggcaaccac
     2941 ttcttcgagg gtgatttcca tgcctaggtc ggccagtttt tcaatcatcc ggatagcccg
     3001 ttcgcgccgg ccgatcagat acatttccat catctccacc agagaagtag aggccagatt
     3061 gaccccgaag gcgagaacat ggacatctga gccttcatgt tgtgaactga tctccatgcc
     3121 gggcaggatc cgcagcggag ctatgtcctt cggcagcatc cgataagcgt ctgcggtgtc
     3181 gtgatctgtt atggagatca ggtccagccc gatttgctgg gcacgcctga ccagctgtgc
     3241 tggactgagc aatccatctg aaacgttggt gtgcaaatgt agattgatga gcttgatggg
     3301 atctatattc ttaactatca taatctcttt ttctattgac gtatttctag ctccccaaaa
     3361 atcg
//
LOCUS       DAOOWR010000184         3343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28585_length_3343_cov_3.223540, whole genome shotgun sequence.
ACCESSION   DAOOWR010000184 DAOOWR010000000
VERSION     DAOOWR010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28585_length_3343_cov_3.223540"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1439)
                     /locus_tag="PLB85_05130"
     CDS             complement(24..1439)
                     /locus_tag="PLB85_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU72748.1"
                     /translation="MKGKVMILEDDLLLAGRIAKLLKVHEYSVLHSKNSDSFFEELRG
                     YRPDVILLDVFLVGSRLNGIQVLKYLKENLDFNYKVIVISGEVTNELVREIRSIGAYH
                     FIEKGANFSINQLLLHIENAVTLKRQEEYNLDLQIEYLNLKKQFTRTFPFIGESEGIN
                     NVRSQLMKFAEVDEDILILGENGTGKEVAANYYYVNSKRFGMPFHTILCSSLNEALIE
                     RELFGRERGTESVHDRGTVGLFERCSEGILFLDEVTNLSLQSQAKILRAVENKEIQVV
                     GGAIKQVNTRLIYTSNAELKTLADSTRIRRDFFYRIEGNVIRIPPLRERGDDILLLMS
                     YFITSYANSYHISEQLDLQALKDELLAYKWPGNVRELKNFCRYISINEREINNKTILK
                     HLHNKILHSHEDSAVGMDRYFKIKNIKDSSAAFERDYILHFLKENDWMVSKTAQMIGL
                     ERTTLYKKIKQLGLASMLENH"
     gene            complement(1450..2646)
                     /locus_tag="PLB85_05135"
     CDS             complement(1450..2646)
                     /locus_tag="PLB85_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOU72749.1"
                     /translation="MLNVVFYFQVHQPYRLKHLNVLDIGKNTDYFDDRLNAQVMRKVA
                     EKCYLPTNKLLLELIQKLEGRFKVAYSITGTAIEQFKAWSPETLDSFKALVDTGCVEL
                     LGETYYHSLAFLYDTNEFLDQIALHRELMQREFGYYCETFRNTELIYQDSLSDLIYEI
                     EGFKTIITEGADRILEWRTPLYAYKNYSKNINLLLKYYQLPDDIAFRFSNRDWPEYPL
                     TVDKFVNWIDHLTLAETEGRNQFLNLFMDYETFGEHQWADSGIFDFMQQFPGEVLKRQ
                     HLGFATPKETFGLANYQQEALSFPTPVSWADAERDLSAWLSNDMQRNAIETLYRLLSL
                     VKRKGDQRLLETIRKLSTSDHFYYMCTKYFTDGDVHKYFSPYDSPDQAYIYYMNAMAD
                     LEERLI"
     gene            complement(2660..>3343)
                     /locus_tag="PLB85_05140"
     CDS             complement(2660..>3343)
                     /locus_tag="PLB85_05140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU72750.1"
                     /translation="FRYKTIYQYFGMSRYRIDTAKIRIVHNAFTLSEESVTSKKRIFK
                     GPTVLFLGRITLQKGPDYFLEVAARVLKVHPEARFIMAGSGDMGRKLLRDSASRKLQN
                     RFLFTGFLNRAQVEDILRASDIYMMPSISEPFGISPLEAMAFGLTSIISKQSGVAEVV
                     NHAFKIDYWDVELMANTVNHLIENPDKCSKMGMDGMREVNQIHWKEAAEKIRLVYSST
                     LAEYIKSNS"
BASE COUNT          682 a          820 c          962 g          879 t
ORIGIN      
        1 taggccaatg ttgtttttca tcatcagtgg ttttccagca tactggcaag gcccagttgt
       61 ttaatctttt tgtagagtgt ggtgcgctcg agtccgatca tctgggcggt tttactcacc
      121 atccaatcgt tttccttcag gaaatgcagg atgtaatcgc gctcgaatgc cgcgctgctg
      181 tccttgatgt ttttgatctt gaaatagcgg tccatgccca cggcgctgtc ttcatggcta
      241 tgcaggatct tgttgtgcag gtgctttagg atggttttgt tgttgatttc gcgctcattg
      301 atgctgatat agcggcagaa gttcttgagt tcgcgcacgt tccccggcca tttgtaggcc
      361 agtagctcgt ctttcagcgc ctgcaggtcc agttgctcgg aaatgtggta gctgttggca
      421 taactggtga tgaaatagct catcagcagg aggatatcgt cgccccgttc cctcagcggc
      481 gggatgcgga tgacgttgcc ttcgatgcgg tagaagaaat cgcggcggat gcgggtggaa
      541 tcagccagag tctttaattc ggcattggat gtgtaaatca ggcgtgtgtt cacctgtttt
      601 atcgctccgc ccaccacttg gatttccttg ttctccacgg cgcgcaggat tttggcttgt
      661 gactggaggc tcaggttggt cacctcgtca aggaagagga taccttccga gcaccgttca
      721 aacaagccca ccgtgccccg gtcatgcacg ctttccgtgc cccgttcccg gccgaaaagt
      781 tccctttcaa tcagggcttc gttcagggaa ctgcataaaa tcgtatggaa gggcatgccg
      841 aagcgtttgg aattcacata gtagtagttg gcagcgactt ccttgcctgt gccgttttcg
      901 ccaaggatga gaatgtcttc gtccacctcg gcgaatttca tcaactggct gcgaacgttg
      961 ttgatgccct cgctttcgcc gatgaaggga aaggtgcgcg tgaactgctt tttcaggttc
     1021 agatactcga tttgcaggtc caggttatat tcttcctggc gcttgagggt caccgcgttt
     1081 tcgatgtgca gcaagagttg gttgatgctg aaattggcgc ctttttcaat gaagtggtaa
     1141 gcgccgatgg aacggatttc gcgcaccagt tcgttggtca cttccccgga aatcacgatg
     1201 accttgtagt tgaaatccag gttctccttc aggtatttca gcacctggat gccgttcagc
     1261 cggctgccca ccagaaatac gtccagcagg atcacatccg gtctatagcc cctcaactct
     1321 tcgaaaaagg aatccgagtt tttggaatga agcacggagt attcatgcac tttcagcagt
     1381 ttggcgatcc ggcctgccag cagcaggtcg tcctcgagga tcatcacttt gcctttcatg
     1441 ctaaggtcct cagatcagcc tttcctcgag gtcggccatg gcattcatgt aatagatgta
     1501 agcctggtcg ggagagtcgt agggtgagaa atacttgtgc acgtcgccat ccgtgaagta
     1561 tttggtgcac atgtaataga aatggtcgga ggtgctcagt ttgcggattg tttcaagcaa
     1621 ccgctggtcg ccttttctct tcaccagtga cagcagcctg tagagggttt caatggcgtt
     1681 gcgctgcatg tcgtttgaaa gccaggcgga aaggtcgcgt tcagcgtcgg cccaggaaac
     1741 aggggtgggg aaggaaaggg cttcctgctg gtagtttgcc agcccgaagg tttctttggg
     1801 ggtggcaaag cccagatgct ggcgcttgag cacttcgccg gggaattgtt gcatgaagtc
     1861 gaagattccg gaatcggccc actggtgctc gccgaaggtc tcatagtcca tgaagaggtt
     1921 caggaattgg ttgcgcccct cggtttcagc cagggtgagg tggtcgatcc agttcacgaa
     1981 tttatccacc gtaagcggat attcgggcca gtcacggttc gagaagcgga aggcgatgtc
     2041 atctggaagc tggtagtatt tcagcagcag gttgatgttt ttggaatagt ttttataggc
     2101 gtagagcggg gttcgccatt ccaggatgcg gtctgcgcct tcagtgatga tggtcttgaa
     2161 accctcgatt tcataaatca ggtcggaaag gctgtcctga tagataagct ccgtattgcg
     2221 gaaggtttcg cagtaataac cgaactcccg ctgcatcagc tccctgtgca gggcgatctg
     2281 gtccagaaac tcgttggtgt catagaggaa agccagggaa tggtagtagg tttcgcccag
     2341 aagctccacg cagccggtat caaccagggc tttgaacgaa tccagcgtct ccgggctcca
     2401 ggccttgaat tgctcaatcg ccgttcccgt gatggaatag gccaccttga agcgtccctc
     2461 cagcttttgg atcagttcca gcaggagttt gttggtgggc aggtagcatt tttccgccac
     2521 tttgcgcatc acctgggcgt tcaggcggtc gtcgaaataa tccgtgttct tgccgatgtc
     2581 cagcacgttc agatgtttga gccggtaggg ctggtgcact tgaaagtaaa aaaccacgtt
     2641 tagcatattg tcacacctgt caggaattgc ttttgatgta ttcagcgagg gtggaggaat
     2701 agaccaggcg gatcttttcc gccgcttcct tccagtggat ctggttgacc tcgcgcatgc
     2761 cgtccatccc cattttactg catttatcgg gattctcgat aagatggttc accgtgttcg
     2821 ccatcagttc cacgtcccag tagtctatct tgaaggcgtg gttcaccact tccgccacgc
     2881 cgctttgctt ggagatgatg gaggtgaggc cgaaggccat tgcctccaga ggcgaaatcc
     2941 cgaagggttc ggaaatcgag ggcatcatat agatgtcgga agcccggagg atgtcctcga
     3001 cctgggcgcg gttcaggaag ccggtgaaaa ggaacctgtt ctgcagttta cgggaggcgg
     3061 aatcgcgcag cagtttgcgt cccatgtctc cgcttccggc catgatgaaa cgtgcctcgg
     3121 gatggacttt cagaacccgc gcggccactt ccaggaagta atccggccct ttctgcaggg
     3181 ttatccggcc caggaagagc accgtggggc ctttgaagat gcgcttcttg gaggtcaccg
     3241 attcctcgga aagcgtaaag gcgttgtgca cgatgcggat cttggctgtg tcgatgcggt
     3301 agcgcgacat gccgaagtac tggtagatcg tcttgtagcg gaa
//
LOCUS       DAOOWR010000185         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28765_length_3325_cov_4.921713, whole genome shotgun sequence.
ACCESSION   DAOOWR010000185 DAOOWR010000000
VERSION     DAOOWR010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28765_length_3325_cov_4.921713"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1265)
                     /locus_tag="PLB85_05145"
     CDS             complement(126..1265)
                     /locus_tag="PLB85_05145"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOU72751.1"
                     /translation="MKCVHCGAEIEADSSFCIVCGKPVSPSRQLRIGRDAGNDIVVNH
                     DRISRLHAVITKEGSHYRITDQNSLNGILVNGQKVTSALIRPGDKVSLGGIVELDWNA
                     VAVAFSGVKSGTRPRPAPVFYHQEASQPRKSPSALLISLIVLGVIGLLAGAYFLWDSI
                     SAGSGKSFNIQSEYSYSVKSVEDPEMASRRSEVETLEDLLARAPAVFGREMSLLLSYC
                     LLDVKVSEPQTLRSSGTEEGKILRTASTSITEREFNARYEYLRGREDHLAMISEAENQ
                     LAQARAELKLREAELSLASKAPEGRDLINQGIGAINVLAKKGDKPPLDSLKDNRIDSS
                     VNQALRDYDEALGNLEGVYASLNDIKAEVAAVKADSSKKGGRVLK"
     gene            complement(1287..2795)
                     /locus_tag="PLB85_05150"
     CDS             complement(1287..2795)
                     /locus_tag="PLB85_05150"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOU72752.1"
                     /translation="MQKNTVRLRSANISDIGRNPARTKNEDYFGHFQGEFGDLFLVCD
                     GMGGHEGGEIASRLAVESIRKHFETNYVPGEELATITQSIDFAQQKITERVELEPELA
                     GMGTTLVLLLIQGVSYYIAHCGDSRIYLSRGGAINQLTKDHSEVQMMVESGIITQEQA
                     ATHPRRNFITKAIGHTSFAPDVSGPYILQQDDVFLLCSDGLTEYVKEDELHQQMEEEP
                     QIACQNLVDMANDRGGNDNITIQIVQVQQCSPFTLREELPTPRRKKILPPLALVVTLL
                     VFLGAVFWYARSIQVPPSPEIAATRDSLRAARAEAKRLAKEEKKRLKEERKKKGKKAS
                     EKAIDPKLAVADSTIANRLQSYAAGNPYLEAFNAMSNKTAQAKNFKFISKSEANQVIY
                     IVPGQTIYLDYAKLAYQAGHNLKSEDITALMAIAVLRSSGQFNLAKEGWESKFIASGT
                     SPIDDQTWNAARELYKKYDSVNADQLFNNPNRFARLRARILVGPYGISLNKG"
     gene            3167..3301
                     /locus_tag="PLB85_05155"
     CDS             3167..3301
                     /locus_tag="PLB85_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72753.1"
                     /translation="MAHGTRAKNPGAADRSGVGIVIDLTDYFIRIIFLVLLYLPANIL
                     "
BASE COUNT          614 a          933 c          974 g          804 t
ORIGIN      
        1 gagcattttg atggcgacct tgcgccccag cagctcttcc tcggcggagt aaaccttgcc
       61 catgccgcct tcgccgatgt aatccagcac acggtagtta cggatgaaat cgccgggttt
      121 gatgatcatt tcaggacgcg gccgcctttc tttgaactgt cggctttaac ggcagccacc
      181 tcggctttga tgtcgttcag ggaagcgtaa acaccttcaa ggttgcccaa ggcttcatcg
      241 taatctctga gggcctggtt caccgagctg tcgatgcggt tgtctttcag gctgtccagg
      301 ggcggcttat cgcctttttt ggccaaaacg ttgattgcgc cgataccctg gttgatgagg
      361 tccctgcctt caggcgcctt gctggccagg ctcagttccg cctcgcgcag cttcaattcc
      421 gccctggcct gggcaagctg attttccgct tcggagatca tggccagatg gtcttctctg
      481 ccccgcaaat actcatagcg ggcgttgaat tccctttccg tgatgctggt gctggccgtg
      541 cgcaggatct ttccctcctc agttccggag gatcgaaggg tctgcggttc gctcactttc
      601 acgtccagca ggcaatagga caggagcagg gacatttccc tgccaaacac ggccggagcc
      661 ctggccagca agtcctccag cgtttccacc tccgagcggc gggaggccat ttccggatcc
      721 tccaccgatt tgacgctgta gctgtattcg gactggatat tgaatgattt gccgctgccg
      781 gcggagatac tgtcccaaag gaaataagcg ccggcaagca gtccaatcac ccccagcacg
      841 atgaggctga ttagcagcgc ggacggggat ttcctgggct gggacgcttc ctggtggtaa
      901 aagaccggtg caggacgggg tcgggtgccg gacttaacgc cggaaaaagc gaccgccacg
      961 gcgttccagt ccaattccac gatcccgccc agggaaacct tgtcgcccgg gcggatcagg
     1021 gcgctggtca ctttctgtcc gttcaccagg atgccgttca gcgagttctg gtcggtgatc
     1081 cggtaatgtg agccctcttt ggtgatcacg gcgtgaagcc gcgaaatgcg gtcatggttc
     1141 acaactatgt cgtttccggc gtcacggcca atccgcagtt gccgggaagg gcttacaggc
     1201 ttgccgcaga caatgcagaa actgctgtcc gcctcgattt ccgctccgca atggacgcat
     1261 ttcatggttt cgttctctcc gtaaggtcag cctttgttca ggctgatgcc gtagggccct
     1321 accaggatgc gggcgcgcag gcgggcgaaa cggtttgggt tgttgaaaag ctggtcggca
     1381 ttgaccgaat cgtatttttt ataaagctcc cgggccgcgt tccaggtctg gtcatcgata
     1441 ggggaagtgc cagaggcgat gaacttgctt tcccagccct ctttggcaag gttgaactga
     1501 ccggaggagc gcagcacggc gatggccatc agggcggtga tgtcctcgga tttcagattg
     1561 tgcccggcct ggtaggccag cttggcataa tccagataga tggtctggcc gggaacgatg
     1621 tagatcacct gattggcttc ggatttgctg ataaatttga agtttttggc ctgcgcggtc
     1681 ttgttgctca tggcgttaaa ggcttccaga taagggtttc ccgcggcgta agactgcagc
     1741 cggttggcga tggtggaatc cgccaccgcc agtttggggt cgatggcctt ttccgaggct
     1801 tttttccctt ttttcttccg ctcttctttc agccgcttct tttcttcttt ggccagacgt
     1861 ttggcctcag ccctggcggc tctcagggaa tctcgggttg ccgcgatttc cggagagggt
     1921 ggaacctgga tggaccgggc ataccagaac acggcgccca gaaagaccag cagcgtgaca
     1981 accagagcca aaggaggcag tattttcttc ctgcgcggag tgggaagctc ttctctgagt
     2041 gtgaagggcg agcactgctg cacctgcacg atctggatgg ttatgttgtc gttcccgccg
     2101 cggtcgttgg ccatatccac caggttctgg caggcgatct ggggttcctc ctccatctgc
     2161 tggtgcagtt catcttcctt cacgtattcc gtaaggccgt ccgagcagag caggaaaacg
     2221 tcgtcctgct gcaggatgta gggtcccgag acgtccggcg cgaagctggt gtggcctatg
     2281 gctttggtga tgaaattgcg ccgaggatgg gtagctgcct gttcctgggt gatgataccg
     2341 ctttccacca tcatctgcac ctcggagtgg tctttggtga gttggttgat cgcgccgccg
     2401 cggctaaggt agatgcggct gtcaccgcaa tgggcgatgt aatagctaac tccctgaatc
     2461 agcagcagaa ccagggtggt gcccatgccc gcaagttccg gctctagttc cacccgttcg
     2521 gtgatctttt gctgggcgaa atctatgctt tgggtgatcg ttgccagctc ttctccgggc
     2581 acgtaattgg tctcaaaatg tttgcggatg ctctccactg ccaggcgcga agcgatttcg
     2641 ccaccctcgt ggccacccat gccgtcacag accaggaaaa ggtcgccgaa ttcgccctga
     2701 aaatggccaa aatagtcttc gttcttggtg cgagccggat ttctgccaat atccgagatg
     2761 ttcgcgctgc ggaggcggac ggtgttcttt tgcataaatc gctccctgcg tattctattc
     2821 cccatgcaga ggggtttttc catgttaaat caaatggccg ggcgttccgc aatatgtcaa
     2881 gttttaattt ttcccctctt tcagacaggg tgggtaaagc aaggaggaca gctttccatt
     2941 gggatgcctc cgctccctcg gtttgaacca aagccatata tgcctgtccg cctcaatgtc
     3001 ctgccagctt gaccctgagc gcccgagtcc attccactcc cctgcctccc gcacaatacc
     3061 cgcaccaaat gcggccattg tacgggaggc gcgtccggga gacaggaaaa ggccttaacg
     3121 gccagaccat gccccttcgt tcgttcgcgg ggaatccggc gcggggatgg cgcatggaac
     3181 gcgggcaaaa aaccccggag cggcggaccg ttccggggtt gggatcgtta tcgacctgac
     3241 ggattacttc atcaggatca tctttctggt gctgctgtac ttgccggcga acatcttgta
     3301 gaaatagacg ccgctggaga caggc
//
LOCUS       DAOOWR010000186         3318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28838_length_3318_cov_3.576770, whole genome shotgun sequence.
ACCESSION   DAOOWR010000186 DAOOWR010000000
VERSION     DAOOWR010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28838_length_3318_cov_3.576770"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLB85_05160"
     CDS             complement(<1..599)
                     /locus_tag="PLB85_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72754.1"
                     /translation="MRTALIFKVALALCLALSLTGLAAAEYRNGQLYIEVLLCSGASI
                     ELEAESSAATKVVVSERESPLSLGIDTPIRISLDNSATLSSWSWLESSRPWDTDSNGV
                     LRESFAWEDSSLVIKRENLVYSEHSFADKATALRFAREAGVSEEKVQGIPLLHATVRV
                     TGAKGAARYLETPLHLSSADPLCLNGDELGFSGEFLLKCV"
     gene            complement(610..2625)
                     /gene="metG"
                     /locus_tag="PLB85_05165"
     CDS             complement(610..2625)
                     /gene="metG"
                     /locus_tag="PLB85_05165"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU72755.1"
                     /translation="MKYLVTSALPYANGKLHIGHVAGAYLPADIFVRWLKLHHEDVIY
                     ICGTDEHGTPISISADQEGVSPAEVVKRYHESIRDAFAALEIEFDNFSGTSRPAHYQL
                     SQDFFSALYDKGHIIPRVTRQFYCKHDKRYLPDRYVEGTCPRCQDPGARGDQCDACGQ
                     IYETTTLIEPRCKICGNKPEIRETTHWYLQLNDFKEHLERWIATKDYWKENVRNFMLN
                     LLEQGLVERPITRDLSWGVPVPLADADDKVLYVWFEAPIGYISSTVEWAERIGQPERW
                     KDYWLDPETRLVHFIGKDNIIFHALIWPAVLMGQNQSYCLPYDIPANEFLNLEGQKLS
                     TSHNWAIWADEIAAKFPAEYIRYYLACIAPEKGDSDFSFRDFQQKVNGELNNVLGNLA
                     NRVFTFSGKNFEGRIAHPQLSPAAKAVLDEALALFNQIDSGYQDYQVKANTRLVMEIA
                     RLGNRFFDERKPWAGIKTDPDAVKETLWTCLTLLRLVSVALFPIMPVSMDRLRDMMGL
                     EPLQEWEQPDLSADYILREAKPLFAKLDDATIEAEIALLHEKAAKAGKPNNAEPIKAQ
                     ISYEDFDRLDIRVATVLEAAPVPKTNKLLRLKVDLGSEQRELVAGIAEHYKAEDVAGK
                     QVLMLVNLEPRSIRGIVSQGMVLAVNTPDALLLVHPAQACPPGSVVK"
     gene            complement(2622..>3318)
                     /locus_tag="PLB85_05170"
     CDS             complement(2622..>3318)
                     /locus_tag="PLB85_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU72756.1"
                     /translation="ACYLQAGRCEIFTDVDGVFTADPRVVPEARLLPQIGYEQMLALA
                     YNGSKVMHPRAVEFASKYQIPVEVKNSRSFAPGTLIHTEKASKEEMMEERKITAIAHK
                     ENLIRLELARDAKLTQLLQDWNNEIFKYSVGENSIELMIEEKYRGEAEYLLRQHDIEA
                     LSWEDGFGYVTLVGLGINLDPGWLARTLELCAPFGIRRVQHSDMTIELMLPSEQVRPC
                     VLKLHQAFIGEEK"
BASE COUNT          647 a          879 c          989 g          803 t
ORIGIN      
        1 acgcatttca gcaggaattc tccgctgaag cccaactcat ccccattaag gcagaggggg
       61 tccgcggaac tgagatgcag cggtgtttcc agataccggg cagccccttt ggcacctgtg
      121 acccggaccg tggcatgaag caagggtatg ccctgaacct tctcctcgct gactccggct
      181 tcccgggcga atctcagcgc cgtcgctttg tccgcgaaac tgtgttctga atagactagg
      241 ttttcccgct tgatcaccag gctggaatcc tcccaggcaa agctttccct caaaactccg
      301 ttggaatcag tgtcccatgg tctgctgctc tccagccagc tccaactgga tagcgtggct
      361 gagttatcca ggctgatcct gatgggggta tcgataccga gtgagagggg tgattccctt
      421 tctgatacaa ccactttggt ggctgcactg ctttcggctt ccaattcgat gcttgccccg
      481 gaacagagca gcacctcgat gtataattgt ccattccggt actcagcagc ggccaagcct
      541 gtcagcgata aagccaggca caaagcaagc gccactttaa atatcagggc agttctcatt
      601 attgtagatc tatttaacca ctgatccagg tggacaggcc tgggcggggt ggaccagcag
      661 cagggcgtcc ggggtgttca ccgccagcac catcccctgc gaaacgatgc cgcggatgct
      721 gcggggttcc aggttcacca gcatcagcac ttgttttccg gctacgtctt cagctttgta
      781 atgttcggcg attccagcca ccagttcgcg ctgttcgctg ccgagatcaa ccttcaggcg
      841 cagcagcttg ttggttttgg gaactggggc ggcttccagg actgtggcca cacggatgtc
      901 caggcggtcg aaatcttcat aggagatctg ggccttgatg ggttctgcgt tattgggttt
      961 tccagccttg gcagctttct catgtaagag ggcgatttcg gcctcgatcg tggcatcgtc
     1021 cagcttggca aagaggggct tggcttcccg caggatgtag tcagcgctga gatccggctg
     1081 ttcccattct tgcagaggtt ccaggcccat catatcgcgc aggcggtcca ttgagacagg
     1141 cataatgggg aagagggcca cggaaaccag ccttagcagg gtgaggcagg tccagagagt
     1201 ttccttcacc gcatcgggat cggtcttgat cccggcccag ggcttgcgtt cgtcgaagaa
     1261 gcggttgccc aggcgggcga tttccatcac cagccgggtg ttggctttca cctgatagtc
     1321 ctggtagccg gagtcgattt ggttgaacaa ggctaaagcc tcgtccagaa cggctttggc
     1381 ggcgggactt agctgcggat gggcgattct gccctcgaag ttcttgccag agaaggtgaa
     1441 aacgcggttg gccaggttgc cgagcacatt gttgagttca ccgttcacct tttgctggaa
     1501 atcgcggaag gagaagtcac tgtctccctt ttccggagcg atgcaggcca gatagtagcg
     1561 tatgtattca gcggggaatt tggccgcgat ttcgtcagcc cagatagccc agttgtggct
     1621 ggtggaaagt ttttgccctt ccaggttcag aaattcgttg gcggggatgt cataaggcag
     1681 gcaatagctt tggttttgcc ccataagcac cgctggccag ataagggcgt ggaagatgat
     1741 gttgtctttt ccgatgaagt ggaccagcct ggtttcggga tccagccagt agtctttcca
     1801 gcgctccggc tggcctatgc gttctgccca ttccacagtg gaggagatat agccaatggg
     1861 ggcctcaaac catacataga gcactttgtc gtcggcatcg gccagaggca cgggcacgcc
     1921 ccagctcagg tcacgggtga tgggacgctc caccagtccc tgttccagca ggttgagcat
     1981 gaagttgcgg acattttcct tccagtagtc ctttgtggcg atccagcgtt ccagatgctc
     2041 tttgaaatcg ttcaattgca aataccagtg ggtggtttca cgaatttccg gcttgttccc
     2101 gcagattttg cagcggggtt cgatgagggt ggtggtttca tagatttggc cgcaagcatc
     2161 gcattggtcg ccacgggcgc cggggtcctg gcagcggggg caagtgcctt ccacatagcg
     2221 atctggcaga tagcgtttgt cgtgtttgca atagaattgt cgcgtcactc tggggatgat
     2281 gtgtcccttg tcatagaggg cgctgaagaa atcctgtgaa agctggtagt gggctggccg
     2341 tgaggtcccg gaaaagttgt cgaattcgat ttccagggcg gcaaaagcgt cccggatgga
     2401 ttcgtgatag cgtttcacca cctcggcggg gctgactcct tcctggtcgg cggagatgga
     2461 aatcggggtg ccgtgttcgt ccgtgccgca gatgtagata acatcctcgt gatgcagttt
     2521 cagccagcgg acgaagatgt cggccggaag ataggccccg gccacatggc cgatatggag
     2581 tttgccgttg gcatagggaa gggcgctggt caccagatat ttcatttttc ttctccaatg
     2641 aaggcctgat ggagtttcag gacgcagggg cgaacctgct ccgaaggcag catcagttcg
     2701 atggtcatgt cgctgtgctg aacacggcgg atgccgaagg gggcacagag ttcaagggtg
     2761 cgggccagcc agccgggatc gaggttgatt cccaaaccca ccagggtaac gtaaccgaag
     2821 ccgtcttccc aggacagcgc ttcaatatca tgttgccgca gcaaatactc cgcttcgccg
     2881 cggtattttt cctcaatcat cagctcgatg ctgttttcgc ccacggagta tttgaatatc
     2941 tcgttgttcc agtcctgcag cagttgcgta agtttggcgt cgcgggccag ttccaggcgg
     3001 atcaggtttt ccttgtgcgc gatggccgtt atcttgcgtt cttccatcat ctcttccttg
     3061 cttgcttttt ctgtgtggat cagggttcca ggcgcgaagc tgcgcgaatt tttcacctcg
     3121 actggaatct ggtatttgct ggcgaattcc accgcccggg ggtgcatcac tttggagccg
     3181 ttataggcca gagccagcat ctgttcatag ccgatttgtg gcagcagacg ggcttcagga
     3241 accacccgcg gatcggcggt gaaaaccccg tccacgtcgg tgaaaatctc gcagcgcccg
     3301 gcttggaggt aacaggcc
//
LOCUS       DAOOWR010000187         3309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_28940_length_3309_cov_4.552858, whole genome shotgun sequence.
ACCESSION   DAOOWR010000187 DAOOWR010000000
VERSION     DAOOWR010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28940_length_3309_cov_4.552858"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..970
                     /locus_tag="PLB85_05175"
     CDS             59..970
                     /locus_tag="PLB85_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOU72757.1"
                     /translation="MKPIRSLEELARHVRGLGTKTRIAVAAAEDPNTISSIARAVSEG
                     FAQGILLGDEERIRAVCAAERIDPTLFEIRPVADPGDAAIEAVKMTRSGEADVLMKGL
                     VGTDKFLKAVLNKETGLLPPKAVMSYVCALELPKYSKLLFISDTAVLPQPDLEQKVAM
                     VRYSVEMARRFGIEKPKVALISATEKISPGMESTLHSALISKMAERGQIKNCVVDGPL
                     DVFLACDPTASKIKGVDSPIAGEADILIFPSLESANSFYKGLMLFGGGELAGLIQGTV
                     KPVVVMSRSESAASKYYCVALSCLMAG"
     gene            972..1412
                     /gene="rpiB"
                     /locus_tag="PLB85_05180"
     CDS             972..1412
                     /gene="rpiB"
                     /locus_tag="PLB85_05180"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU72758.1"
                     /translation="MKIAIASDHAGYELKEAIKAAFPEHEFEDFGTHSVDSMDYPDTG
                     APAAQAVAAGKAERGILICGSGIGMSITANKVRGIRAALCTNTDLARLSRIHNDANVL
                     CLAGRFTAVPYALEIVNNWLNSAFEGGRHQNRIEKIKLLEGDKQ"
     gene            1409..2704
                     /gene="glyA"
                     /locus_tag="PLB85_05185"
     CDS             1409..2704
                     /gene="glyA"
                     /locus_tag="PLB85_05185"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU72759.1"
                     /translation="MKHIQRQDPELYAAIAAELKRQRENLELIASENFTSMAVMEAQG
                     SVLTNKYAEGYPYRWSKKTGKINYNLYGRYYGGCEYIDEVERLAIERAKQLFGSEHAN
                     VQPHSGSQANMAAYFTLVKPGDTVLSLELAHGGHLTHGHPLSFSGQFYNIIHYNVNKD
                     TEQFDYDELEALAKEHKPQMILAGASAYPRKMDFARFREIADMVGAKLMVDMAHIAGL
                     VAAGLHMNPVPYSDVVTSTTHKTLRGPRAGIILCKEEYAKEIDGKVFPGIQGGPLMHA
                     IAGKAAALYEALQPEFKAYQKQVIDNAQALAAALTQHGLKLVSGGTDTHLMLMNMGTE
                     EEGGPSGKKMEEALDKAGITANKNTVPFDTRSPFVASGVRLGTPSVTTRGMGVDEMKL
                     VADFIKRVRDNHTDEEYLAAMKAEVRELTDKFPLYPELG"
     gene            2968..>3309
                     /locus_tag="PLB85_05190"
     CDS             2968..>3309
                     /locus_tag="PLB85_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72760.1"
                     /translation="MNINLAELPGKLGAFGIKLAIALVIFIVGNWIAKAVSKALCGLM
                     LKRKADKTIATFIGNIVYAVLLLIVVLTALNRLGVQTSSFMVIVSAAVLAIGMSMQGT
                     LGQFASGVMLIG"
BASE COUNT          779 a         1002 c          925 g          603 t
ORIGIN      
        1 aacgctccaa actctattcc atcgccctgg cggccctgct ggcgggagcg cggcagggat
       61 gaaacccatc cgctctctgg aagagctagc ccgccacgtc cggggactgg gcaccaaaac
      121 ccggatagcc gtggccgccg cggaggaccc aaataccatt agttccattg ccagagcggt
      181 ttccgaaggt ttcgcccagg gcatcctgct gggcgatgaa gagcggatca gggcagtttg
      241 cgcggctgag agaatcgacc ccaccctgtt tgagatcagg cccgtggccg atcccggcga
      301 cgccgccatt gaggcagtaa aaatgacccg tagtggggag gcggatgtgc tgatgaaagg
      361 cctggtggga acggacaagt tcctgaaagc tgtgctgaac aaggaaacag gcctgctgcc
      421 ccccaaggcg gtgatgagct acgtctgcgc gctggagctg cccaaatatt ccaaactcct
      481 cttcatcagc gacacggcgg tgctgcctca gcccgacctg gagcaaaagg tcgccatggt
      541 gagatacagc gtggagatgg cccggcgctt cgggatcgaa aaacccaaag tcgccctcat
      601 ttccgccacc gaaaagattt caccgggcat ggaaagcacc ctgcacagcg ccctcatctc
      661 caaaatggcc gagcggggcc agatcaaaaa ctgcgtggtg gacgggccgc tggacgtttt
      721 tctggcctgt gaccccaccg ccagtaagat caagggtgtg gacagcccca tcgccggaga
      781 agcggacatc ctcatctttc ccagcctgga aagcgccaac agcttttaca aaggcctgat
      841 gctcttcgga ggcggcgaac tggccgggct gatccaggga accgtgaaac ctgtggtcgt
      901 gatgagcaga agcgaaagcg cggcctcaaa atattattgc gtggcacttt cgtgcctcat
      961 ggcaggttaa aatgaaaatc gcgattgcga gcgaccacgc cggctacgaa ctcaaggaag
     1021 ccatcaaagc ggcctttccg gaacacgagt ttgaagattt cggcacccac tcggtggatt
     1081 cgatggatta tcctgacacc ggcgcccccg ctgcccaagc cgtggcagcc ggcaaagccg
     1141 aaaggggcat cctgatctgc ggcagcggaa tcgggatgag catcacggcc aacaaggtcc
     1201 ggggcatccg tgccgctctt tgcacaaaca ccgatctggc ccgcctttcc cgtatccaca
     1261 acgacgccaa cgttctctgc ctggcagggc gcttcacggc agtcccctac gccctggaga
     1321 tcgtgaacaa ctggctgaac tcggcctttg aaggcggccg gcaccaaaac agaattgaaa
     1381 aaataaagct actggaagga gacaaacaat gaaacacatc caaaggcaag atcccgagct
     1441 ctacgcggct atagcggcgg aactgaagcg ccagcgcgag aatctggaac ttatcgcctc
     1501 cgaaaacttc acctcaatgg ctgttatgga ggcccagggc agcgtcctga ccaacaaata
     1561 cgctgaagga tacccctacc gctggagcaa aaagaccggc aagatcaatt acaacctcta
     1621 tggccgctac tatggcggct gcgaatacat cgacgaggtg gagcgcctgg ccatcgaacg
     1681 cgccaaacag ctttttggca gcgaacacgc caacgttcag ccccacagcg gttcccaggc
     1741 gaacatggct gcctatttca ccctcgtgaa acctggcgac actgtccttt ccctggaact
     1801 cgcccacggc ggacacctca cccacggcca cccgctttcc ttttcgggac agttttacaa
     1861 catcatccac tacaacgtga acaaagacac cgagcagttc gactatgacg aactggaggc
     1921 cctggcgaag gagcacaaac cgcagatgat cctggccggc gccagcgcct atccccgcaa
     1981 gatggatttc gcccgcttcc gggaaatcgc cgacatggtg ggcgccaagc tgatggtgga
     2041 catggcccat atcgccggtc tggtcgccgc cggcctgcac atgaacccgg ttccctactc
     2101 ggacgtggtt acctccacca cccacaagac cctgcgcggg ccccgggcgg gaatcatcct
     2161 ctgcaaggag gaatatgcca aagagatcga cggcaaggtg tttcccggca tccagggcgg
     2221 cccgctgatg catgccatcg ccggcaaagc cgccgcgctt tacgaagctc tccagcctga
     2281 attcaaggcc tatcagaaac aggtcatcga caacgcccag gctctcgccg cggctttgac
     2341 ccagcatggg ctgaaactgg tttccggcgg cacggacacg catctgatgc tgatgaacat
     2401 gggcacagag gaagaaggtg gccccagtgg caaaaagatg gaggaagccc tcgacaaagc
     2461 cggaatcacc gccaacaaaa acaccgtccc ctttgacacc cgcagcccct ttgtggcttc
     2521 cggagtgcgt ttgggaaccc cctccgtgac caccaggggc atgggcgtgg acgagatgaa
     2581 gcttgtcgcg gatttcatca aacgcgtgcg cgacaaccat accgacgaag aatacctggc
     2641 cgcgatgaag gccgaggtgc gcgaactaac agataaattt ccgctttatc cggaactggg
     2701 ctgagcccgg gttttatata ggcaagggcg gactctgctc cgcccttttt cttttcgctg
     2761 gagtcaagtg aggaacgagc atcgactcca gcaggtcatc ctgccgtcca tgcccacttc
     2821 ttccgctgga cgccaggctg gcgtgaaaag ctgaacctgt gtgacagtta tccgctgaat
     2881 atcttggcaa tgaagcaaat ccggattata tttgcaatat atcacagata attccgcggc
     2941 gacaatgccg cattaaggag tataagaatg aatatcaacc tggcagaact tcctggaaaa
     3001 ctgggagctt tcgggatcaa gctggccatc gccctggtca tcttcatcgt cggcaactgg
     3061 atagccaaag ccgtgagcaa ggcgctttgc ggcctgatgc tcaagcgtaa ggcggacaag
     3121 accatcgcta ccttcatcgg caacatcgtc tatgccgtcc tgctgctcat cgtggtttta
     3181 accgcgctca accgcctggg cgtgcaaact tcctcattca tggtcatcgt cagcgccgcc
     3241 gttttggcca tcggtatgtc catgcagggc acgctgggcc agtttgccag cggggtgatg
     3301 ctgatcggg
//
LOCUS       DAOOWR010000188         3299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29066_length_3299_cov_3.195438, whole genome shotgun sequence.
ACCESSION   DAOOWR010000188 DAOOWR010000000
VERSION     DAOOWR010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29066_length_3299_cov_3.195438"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLB85_05195"
     CDS             <1..1033
                     /locus_tag="PLB85_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOU72761.1"
                     /translation="AQQLQKLGHHTLFIGNRRGMEETLLTAEGYPFHGIRVQKLYRKL
                     SLANLLFPFLLVSSTFACVRVLRKTRPAAVICTGGFVSGPVGIAAALLRLPLYFHECN
                     SYPGITTKYLARYTRVVFTGFSGTAKYLPKAKVCTIGIPLPKREDLPQTFSAAKFSLT
                     EDKPVLLVTGGSQGSLAINKAVADALPHLTQQGWQIVWQTGKTGYDEFAARFKNIAGV
                     YIFAFSPQLKDFYKLARVAVTRAGAMTITELVENRLPAVLIPLPTAAENHQHYNALEQ
                     QQKGLALLLKQSQLSGDTLAEAVFKVDKEHAAYLLNLNQTPPNTAAEDIAREILSDLN
                     KEQNNAGKN"
     gene            1017..>2127
                     /gene="murC"
                     /locus_tag="PLB85_05200"
     CDS             1017..>2127
                     /gene="murC"
                     /locus_tag="PLB85_05200"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU72762.1"
                     /translation="MLGKTKKIHLIGIGGIGMSGIAEFLHNQGLEISGSDLKKTDVTR
                     HLESLGIRIEEGHNPALVKDVDVVVKSSAVKDDNPEVQAAKTLRIPLIRRAEMLAEIT
                     RMSFSIGISGTHGKTTTTSMTGLVLEAAGLEPTIIVGGKVKNYGSNNVMGSGKYIVVE
                     ADEYDHSFLSLAPCIAGITNIDTDHLDCYRNLDDIKGAFIEYANKVPFFGSVIACLDD
                     PGVQAILPSINKKVVTYGFSRQADIQGKNISMKDFVSEYDVSYKGYRLGRVKMNVTGR
                     HNIQNSLQAISIGLELDIPFKHIREGLMQYSGVYRRFELKGEARGITVYDDYAHHPTE
                     ISATLEGFKDSTKRRIVTLFQPHLYSRTRDFYVQFG"
     assembly_gap    2128..2137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2138..2423
                     /gene="murC"
                     /locus_tag="PLB85_05205"
     CDS             <2138..2423
                     /gene="murC"
                     /locus_tag="PLB85_05205"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Catalyzes the formation of
                     UDP-N-acetylmuramoyl-L-alanine from UDP-N-acetylmuramate
                     and L-alanine in peptidoglycan synthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOU72763.1"
                     /translation="LILAPIYPAREEPIPGVSSKLIADIAVQSGHPDVVLIENDADIV
                     PKTLSLLKKDDILITMGAGNIWQYGEEILAKLKKPVDKNIKPKKVRNLET"
     gene            2463..3242
                     /locus_tag="PLB85_05210"
     CDS             2463..3242
                     /locus_tag="PLB85_05210"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOU72764.1"
                     /translation="MRDNKLPINQNTRKRRGNSRYFVYFVLILLGLAALGFGAFHLLS
                     SLDVLNVRKIEISGNSAVADSLLRSTLRPYIGKNLLKVDKDEILKEVSGFARVKDVKV
                     RRKLFSTLQIKITERKASLYVKSLDGDLFPVDDEGVVLERYGKVYTENLPLVNLLLNN
                     SSLKSGFKLKNAALDKVLATHKRIMKEAPEFAANVSEYYTIDNTVYIIDARNGLRLIP
                     SEKNLAKQLSRYEFVRDNGNISQSSILDLRTDNQVVVKAGS"
BASE COUNT          865 a          965 c          797 g          662 t
ORIGIN      
        1 cgcgcagcaa ctgcagaagc ttggccatca caccctcttc atcggcaacc ggagaggcat
       61 ggaggaaacg ctgctcaccg ctgaaggcta tcctttccac ggcatccgag tgcaaaagct
      121 ttaccgcaag ctcagcctcg ccaatttgct ctttccgttc ttgcttgtgt cctccacttt
      181 tgcctgtgtc agagtgctac gcaagaccag gcccgcggct gtgatctgca ccggcggctt
      241 cgtttcggga cccgttggca tcgccgcggc tttactccgc cttcccctct attttcatga
      301 atgcaacagc tatccgggca tcaccaccaa atatctggcc agatacaccc gcgtcgtttt
      361 cactggcttc agcggcactg ccaaatacct gcccaaggcg aaagtgtgca ccatcggcat
      421 tccgttgcct aaaagggagg atttgcccca aaccttcagc gcggcaaaat ttagccttac
      481 ggaggacaag ccggtattgc tcgtcaccgg tggcagtcag ggctcgctgg cgatcaacaa
      541 agccgtggcg gatgccctgc cccacttaac acagcaaggc tggcaaattg tatggcaaac
      601 cggaaagacc ggctacgatg aattcgcggc acgtttcaaa aacatcgccg gagtgtatat
      661 cttcgcattt tcaccccaac tgaaagattt ttataaacta gccagagtgg cagtaacccg
      721 tgctggcgcg atgaccatca ctgaactggt cgaaaaccgt ctgcccgccg tgctgatacc
      781 cctcccaacc gcggctgaga accatcagca ctacaacgcc ctcgaacagc agcaaaaagg
      841 cctggccttg ctgctgaagc aaagccagct cagcggagac acgctggcgg aagcagtgtt
      901 caaagttgac aaagagcacg ctgcctatct gctgaacttg aatcaaacac cgcccaacac
      961 cgcggctgaa gacattgcgc gggagatatt gagcgacctc aacaaggagc aaaacaatgc
     1021 tgggaaaaac taagaagatc catttaatcg gcatcggcgg catcggcatg agcggcatcg
     1081 ccgaattcct gcacaaccag ggcttggaaa tcagcggttc cgacctcaag aagaccgacg
     1141 tcacccgcca tctagaatcg cttggcatca ggatagaaga aggtcacaac cccgccctgg
     1201 tgaaggatgt ggatgtggtg gtaaaatcct cggccgtcaa ggacgacaat cccgaggtcc
     1261 aagccgcaaa aaccttgcgc atacccctca ttcgccgtgc ggaaatgctg gcagagatca
     1321 cccgcatgag cttctccatc ggcatttccg gaacccacgg caaaaccaca accacctcca
     1381 tgaccggact cgtgcttgag gcagcggggc tcgaacccac catcattgta ggcggaaagg
     1441 tgaaaaacta cggttccaac aatgtgatgg gttccggaaa atacattgtg gtggaggccg
     1501 acgaatacga ccattccttc ctctcccttg caccctgcat cgccggcatc acaaacatag
     1561 acacagacca tctggattgt taccgtaatc tggatgacat caaaggcgcc ttcatcgaat
     1621 acgcgaacaa agtgcccttt ttcggcagcg tcattgcctg tcttgacgac ccaggtgtgc
     1681 aggccattct gcccagcatc aacaagaagg tcgttaccta tggcttttcc cgccaggcgg
     1741 acatccaggg caaaaacatc agcatgaaag actttgttag cgaatacgac gtcagctaca
     1801 aaggctaccg cctcggccgc gtgaagatga atgtaactgg ccgccacaat atccagaact
     1861 ccctgcaggc catcagcatc ggccttgagc tggatatccc cttcaagcac atccgcgagg
     1921 ggctgatgca atacagcggg gtttaccgcc gctttgaact caaaggcgag gctcgcggca
     1981 tcaccgttta cgacgattat gcccaccacc ccaccgaaat atccgccacc ctcgagggat
     2041 tcaaggacag caccaaacgc cgcattgtaa ccttgttcca accccatctg tattcccgca
     2101 ccagagattt ctacgtgcag ttcggaannn nnnnnnncct gattctggcg ccgatttatc
     2161 cagctcggga agagcccatc cccggtgttt cctccaaact cattgccgac atcgccgtcc
     2221 aaagcgggca tccagacgtt gtgttgatag aaaacgacgc cgacattgtg cccaaaaccc
     2281 tctccctgct taagaaagac gacattctca tcaccatggg agccggcaac atctggcaat
     2341 atggcgaaga aatcctggca aaactgaaaa aacctgttga caaaaacatt aaacctaaaa
     2401 aagttaggaa cttagagact taacacagga atgcgcgata ctgcacaaaa gcatacatca
     2461 ttatgagaga caataagttg ccgatcaacc agaatacccg caaaagaagg ggaaatagcc
     2521 gttatttcgt ctatttcgtc ctgattttgc ttggtcttgc tgcccttggt ttcggcgctt
     2581 tccatctcct ctccagtttg gatgtgttga atgtgcgcaa aatcgagata agcggcaatt
     2641 ccgctgtagc ggacagcctc ttgagaagca ctctgcggcc ctacatcggg aagaacctcc
     2701 tcaaagtgga taaggatgag atcctgaagg aagttagcgg ctttgcccgc gtgaaggatg
     2761 tgaaggtccg ccgcaagctt ttcagcaccc ttcagatcaa gatcacggaa cgcaaagcca
     2821 gtctgtatgt gaaaagcctc gatggcgacc tctttcccgt ggatgacgaa ggcgttgtgc
     2881 tggaacgcta cggcaaagtt tacacggaaa acctgccctt ggtgaatctg ctgttgaaca
     2941 actccagcct gaaaagcggt tttaagctaa agaacgcagc gctggacaag gttttggcca
     3001 cccacaaaag gataatgaag gaagccccgg aattcgcggc caatgtctca gaatactaca
     3061 ccatcgacaa cacggtctat atcatcgacg cgcgcaacgg cctgcgcctc atccccagcg
     3121 aaaagaacct cgccaagcag ctttcgcgct acgaattcgt gcgtgacaac ggtaacataa
     3181 gccaatcctc aatcctggac ctgcgcaccg acaaccaggt ggtggtgaag gcgggaagct
     3241 gacatgcggc aaaacctgat caccgccctc gacatcggct ctgccaacat ccgctgcat
//
LOCUS       DAOOWR010000189         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29188_length_3290_cov_4.029057, whole genome shotgun sequence.
ACCESSION   DAOOWR010000189 DAOOWR010000000
VERSION     DAOOWR010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29188_length_3290_cov_4.029057"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLB85_05215"
     CDS             complement(<1..658)
                     /locus_tag="PLB85_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72765.1"
                     /translation="MNFRFLLILLLAILTLLGWTFVHHQYLSSRQARLEQIAELPVYV
                     YMNEPARLDSLYYQLKHEVPEIDSLVRETGLQAVEELLQNYPDLGIPANAQQELPNIL
                     TLWFKPSDAAFRGREKVLQILTAKGLKGEDVDSQELAWGHARQELDALSSRWSNSTLF
                     VALLVFLMLVYARLFLYLSDAAANRGMRATVLESIRAGEAAKWQNSLLIVVPVLVSFG
                     L"
     gene            complement(660..1262)
                     /locus_tag="PLB85_05220"
     CDS             complement(660..1262)
                     /locus_tag="PLB85_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU72766.1"
                     /translation="MELWPGGPRIRQHQQAQPVSHASVALQQAALDIFGPDPRRVLDL
                     GSGCGIVALMLALRRPLWQVEGLEVQAELNDLACGNAALCRVNILFHSGDMRSFTAFL
                     PYDLIVSNPPWLPPGSGKTSPIQARNLSRFELLGGLADVLACVKRNLAPAGQALLLYP
                     RSRSGELGKAADKTFLDIISLFPASGLQEHIICRIRHKGQ"
     gene            complement(1274..2176)
                     /gene="nadA"
                     /locus_tag="PLB85_05225"
     CDS             complement(1274..2176)
                     /gene="nadA"
                     /locus_tag="PLB85_05225"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOU72767.1"
                     /translation="MNIAEKIDKLRKEKGALILAHNYQIAPIQDLADHRGDSLHLSMV
                     AREAKNRLIVFCGVRFMAETASILNQKAKILLPVADAGCPMADMITAEQLVAFKAEYP
                     GAKVVCYVNSSAAVKSESDVCCTSANAVKILSSFPKNETILFVPDQNLGTWAAKQAKR
                     KVIVWPGYCPIHQWGFSFRNLEKIRAANPGYRLIAHPECDEDIVKEADAVLSTGQMMR
                     FVENNDRLIIATDSTFVDYLKHIYPNKELVPLNRRARCSNMRKITLNEVLLALEEEQF
                     EVRVPDDMASRAKLCLDRMLELSI"
     gene            complement(2193..2627)
                     /gene="dut"
                     /locus_tag="PLB85_05230"
     CDS             complement(2193..2627)
                     /gene="dut"
                     /locus_tag="PLB85_05230"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOU72768.1"
                     /translation="MIIRFKLLSPNALPPQRMTENSSGWDLSADLSEPEILKPGARAA
                     IPTGITVEIPRGFEGQVRPRSGLALHQGLGVLNSPGTIDADYRGEVKVILFNSSAETV
                     TIQPRMRVAQLVICPVVEASFVESGELGESGRSDGGFGHTGH"
     gene            complement(2624..>3290)
                     /locus_tag="PLB85_05235"
     CDS             complement(2624..>3290)
                     /locus_tag="PLB85_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU72769.1"
                     /translation="AIEMLHTFSLIHDDMPDLDNDEYRRGKKSTHALFGEGHALLAGD
                     ALLVGAFELITYADIKPKIRVQMVRELAHETGVTGLIGGQMVDLESEGRKVDKKTIDY
                     IHENKTAKLIKLCLRFGALAGGAPEEEQKALEEYGHKIGVAFQIIDDLLDIEGDPDEM
                     GKTVGKDAHVQKATYPSVHGMAESRRKAQELISQAREAIAPLGEKAEVLDTLAEYMLT
                     RKS"
BASE COUNT          661 a          919 c          981 g          729 t
ORIGIN      
        1 acaatccgaa ggacaccagc acgggcacca cgatcaggag actattttgc cacttggcgg
       61 cctcaccagc cctgatgctt tccagcactg tggcccgcat cccgcgattg gctgcggcat
      121 cggaaaggta gaggaagaga cgggcgtaaa ccagcattag aaagaccaac agcgccacaa
      181 agagggtgga attgctccag cgggagctga gggcgtccaa ttcctgccgg gcatggcccc
      241 aggcaagttc ctggctatcg acgtcttctc ccttcaggcc tttggcagtg aggatttgca
      301 gcactttttc tcggccccgg aaagcggcgt ctgagggctt gaaccagagg gtgaggatgt
      361 tgggcaattc ctgctgggcg ttggccggga ttcccagatc gggatagttt tgcagcaatt
      421 cctcaacggc ctggagaccg gtttcgcgga ccagggaatc tatctcgggt acctcatgct
      481 tcaactggta atagaggctg tcgagacggg cgggttcatt catgtaaaca tagaccggca
      541 gttccgcgat ctgttccagc cgggcctggc ggctgctaag gtattgatgg tggacgaaag
      601 tccagcccag tagggtcagg atcgccaaca gcagtatcag caggaatctg aaattcattt
      661 cactgtcctt tatgccggat acggcatatg atgtgttctt gcaaaccaga ggctggaaag
      721 agggatatta tgtcaagaaa agtcttgtct gcggctttgc caagctcgcc ggagcgggag
      781 cgcggataga gcagaagggc ctggcctgcc ggggccagat tgcgcttcac gcaggccagc
      841 acatcagcca gcccgccgag caactcaaag cggctgaggt tgcgggcttg gatgggactg
      901 gtttttccgc ttcccggcgg cagccaggga gggtttgaaa cgatgaggtc gtaaggcagg
      961 aaagctgtga atgagcgcat atcgccagag tggaaaagga tattgacacg gcacaaagca
     1021 gcgttgccac aggccaggtc gttcaattcc gcctgcacct ccaggccttc tacttgccag
     1081 aggggacgcc tgagagccag catcagggca acgattccgc agccgctgcc aagatcgaga
     1141 acccgccgag ggtcagggcc aaagatgtcc agcgccgcct gctgcagcgc gacggaggca
     1201 tgggaaacag gctgcgcctg ctgatgctgc cgaatcctgg gccctcccgg ccacagctcc
     1261 accagcaaag aactcagatt gaaagctcca gcatgcggtc caggcagagc ttggctcgac
     1321 tggccatgtc gtccggcacc cgcacctcga attgttcttc ctccagcgcc agcagcactt
     1381 cgttcagggt gatcttgcgc atattgctgc aacgggcgcg gcggtttagc ggcaccagtt
     1441 ccttgttggg ataaatgtgt ttcagataat cgacgaaagt ggagtccgtg gcgattatca
     1501 gcctgtcgtt attctccacg aagcgcatca tctgcccggt ggagagcacc gcgtcggctt
     1561 ccttaactat gtcctcatcg cactccggat gggcgatcag acggtatccg ggattggcgg
     1621 cacggatttt ttcgaggttg cggaagctga aaccccactg gtgaatgggg cagtagcctg
     1681 gccagactat caccttgcgt ttggcctgtt tagccgccca agtgcccaga ttctggtccg
     1741 ggacgaagag gatggtttca ttcttgggaa aagaggaaag gatcttcacg gcgtttgcgg
     1801 aggtgcaaca gacgtcactt tccgatttga cagcagcgct cgaattcacg tagcagacaa
     1861 ccttcgcacc cggatactcg gctttgaagg caaccagttg ctcggccgtg atcatatccg
     1921 ccatggggca gccggcatct gcaaccggca gcaatatttt cgccttctgg ttgaggatgg
     1981 aggcggtttc cgccatgaag cgcactccgc aaaaaacgat aagtctgttc ttggcttccc
     2041 tggccaccat ggagaggtgc aaagaatccc cccggtggtc agccaggtct tggatggggg
     2101 ctatctgata gttatgcgcc aggatgagag cccctttttc cttgcgcagt ttgtcgattt
     2161 tttccgcgat attcacctta tctccctttc cttcaatgcc ctgtgtgtcc gaagcctccg
     2221 tcggacctgc cactttcccc gagttcccca ctttccacaa agcttgcctc aaccaccggg
     2281 caaatcacca gttgcgccac gcgcatgcgg ggctggatgg tcaccgtttc ggcggagctg
     2341 ttgaaaagga tcaccttcac ttcaccgcgg tagtccgcgt cgatggttcc agggctgttc
     2401 aaaacgccca gtccctgatg cagggcaagt ccgctgcggg gacgcacctg gccttcaaag
     2461 ccgcggggta tttccacagt gattcccgtt gggatagcag cccgggcgcc cggcttcaaa
     2521 atctctggtt cactcaggtc cgcgctcagg tcccagcccg agctgttctc agtcatgcgc
     2581 tgaggcggca gggcattggg gctgagcagt ttgaagcgga ttatcatgat ttgcgggtga
     2641 gcatatattc agccagggta tccaggactt cggctttttc gcccagggga gcgattgcct
     2701 cacgggcttg ggaaatcagt tcctgggcct tgcggcgaga ttccgccatg ccgtgcaccg
     2761 aggggtaggt ggctttctgg acgtgggcgt ccttgccaac cgtttttccc atttcgtcgg
     2821 gatcgccttc aatatcgagc aggtcatcga ttatctggaa agccacgcca attttgtgtc
     2881 cgtattcttc caaagccttc tgctcttcct ccggagcgcc accggccaga gcgccgaagc
     2941 gcaggcagag cttgatcagt ttggcggtct tgttttcgtg gatatagtcg atggttttct
     3001 tgtccacttt cctgccttcg gattctaaat ccaccatctg gccgccgatg aggccggtca
     3061 ccccggtttc gtgggcaagt tcgcgaacca tctgcacgcg gatcttcggc ttgatatcgg
     3121 cgtaggtgat gagctcgaaa gcccccacca gcaaagcgtc tccggccagg agggcgtggc
     3181 cttcgccaaa gagggcatgg gtggatttct tgccgcggcg gtattcgtcg ttgtcgaggt
     3241 ccggcatgtc gtcgtgaatg agggaaaacg tgtgcagcat ctcgatggcg
//
LOCUS       DAOOWR010000190         3285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29240_length_3285_cov_3.435604, whole genome shotgun sequence.
ACCESSION   DAOOWR010000190 DAOOWR010000000
VERSION     DAOOWR010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29240_length_3285_cov_3.435604"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..567)
                     /gene="rplQ"
                     /locus_tag="PLB85_05240"
     CDS             complement(160..567)
                     /gene="rplQ"
                     /locus_tag="PLB85_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU72770.1"
                     /translation="MRHRHEGRKFGREKDARRLMICNLVRSMIEHGQITTTLAKAKEL
                     RGFVERVVTYGKTDTVHSRRLAYSVLGSRTLVKKLFTEIAPAFATRQGGYTRVLKAGF
                     RRGDSAPMAIIQFVEEAAVKPKKDNIKAKDLNK"
     gene            complement(557..1690)
                     /locus_tag="PLB85_05245"
     CDS             complement(557..1690)
                     /locus_tag="PLB85_05245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU72771.1"
                     /translation="MLYLEPLQMPENVDYDRETYSHQYGRFEIGPLEPGFGTTIGNTL
                     RRVLLSSIQGSAVRFVRIEGLHHEFTPIPGTNSDYIDLILRLKQLVIYSSSVDEVNLV
                     LEHKGKGVVTAAKIQDNPNVRIINKDLYLLEVTEDVDFKLEMIVGNGRGYVSADRQNP
                     EGKPVGFIPIDSVYSPILKVNFSVTHQRVKERMNFDKLILEIHSNGSVEPIIALFLSA
                     KILRDMVGKICRFETEPEYIRDVHIDPELEEKERILSLPVREIELTVRAANCLDAANI
                     QTIGELVSKTEAEMLKFRNFGKKSLEEIVTKLKKYDLHLGMDVEGIYSQIRDARSRGI
                     KSEDTPEEQKVEETPAPAEPQDEVPAPPRKPVPPSKKKVKNAT"
     gene            complement(1715..2332)
                     /gene="rpsD"
                     /locus_tag="PLB85_05250"
     CDS             complement(1715..2332)
                     /gene="rpsD"
                     /locus_tag="PLB85_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU72772.1"
                     /translation="MARYTGPRAKLCRKFGENIFGTAKYDRILNRRKFPPGQHGRNMR
                     RKPSDYFIHLREKQKLRNIYCLLEKQFSNYFVKAAKMGGVTGDNLLQMLERRLDNTVY
                     RLGFATTRMQARQFVNHGHFLVNGKKVDIPSYLLKDGDIVEVRPKSKGIKPLAEAWNN
                     SEASSPFPWLTVDKENMRGQFVNIPLAAEIPNTVDLRLIVEFYSK"
     gene            complement(2345..2734)
                     /gene="rpsK"
                     /locus_tag="PLB85_05255"
     CDS             complement(2345..2734)
                     /gene="rpsK"
                     /locus_tag="PLB85_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU72773.1"
                     /translation="MAKKTRIKKKRVRLSFDEGIVFVHSSFNNTIVSLADRSGNILTW
                     SSGGRVGYKGSRKATPFAAQLAATEVSKTGLEMGLTRVGVIVRGPGSGRESAIRAVNA
                     SGLKVTMIKDATPIPHNGCRPPKTRRI"
     gene            complement(2749..3117)
                     /gene="rpsM"
                     /locus_tag="PLB85_05260"
     CDS             complement(2749..3117)
                     /gene="rpsM"
                     /locus_tag="PLB85_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU72774.1"
                     /translation="MAHIAGIEIPKDKRLFIGLTYIYGIGRSIARDICRKANVDEMKK
                     VEDLSIEEEKIIRDIVQNEYVVEGALRTQVAMNIKRLMEIGSYRGLRHKRGLPVRGQR
                     THTNARTRKGPKPGAIKKKK"
     gene            complement(3129..3245)
                     /gene="rpmJ"
                     /locus_tag="PLB85_05265"
     CDS             complement(3129..3245)
                     /gene="rpmJ"
                     /locus_tag="PLB85_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOU72775.1"
                     /translation="MKVRSSVRVVCKDCRIIKRHGVIRVICSSNPKHKQRQG"
BASE COUNT          623 a          790 c          993 g          879 t
ORIGIN      
        1 ggaggttgaa aaggggccgt ggaaagggaa ttgatgaatt gatggaattg atgaaatgaa
       61 aatttgggct cgccagtgac catgacggtc ggggtaaagg cggaaacaaa aaacccggaa
      121 accaagttcc gggttatatg cgttcaggcc agaacgggct tacttgttga ggtccttggc
      181 cttgatattg tcttttttgg gtttcacagc cgcttcctcc acaaactgga taatggccat
      241 gggggcggag tcgccgcggc ggaaaccggc tttcagcact ctggtgtagc cgccctggcg
      301 ggtggcaaag gcaggggcga tctctgtgaa gagcttcttc acgagggttc ggctgccaag
      361 gacgctgtaa gccagacggc gggagtggac ggtatcggtt ttgccgtagg tgaccacgcg
      421 ttcgacgaag ccgcggagtt ccttggcttt ggcgagggtg gtggtgatct ggccgtgttc
      481 gatcatggat ctcacaaggt tgcagatcat caggcggcgg gcgtcctttt cgcgcccgaa
      541 ttttcttcct tcatgtctat gtcgcatttt tcaccttctt ttttgaggga ggaaccggtt
      601 tgcggggcgg ggcgggaacc tcgtcctggg gctcggcggg agcgggtgtt tcctccactt
      661 tctgttcttc gggggtgtct tcagatttga tgccacggct tctggcgtca cggatctggc
      721 tgtaaattcc ttccacatcc atacccaggt gcaggtcgta tttcttcagc ttcgtcacaa
      781 tctcttcaag ggatttcttg ccgaaattgc ggaacttgag catctccgcc tcggttttgg
      841 agaccagttc gccgatggtt tggatattgg cggcgtcgag gcagttggca gcccggacgg
      901 tgagttcgat ttcacgcaca ggcagggaaa ggatgcgttc cttttcctcc agttctggat
      961 cgatatggac gtcacggatg tactccggct cggtttcgaa gcggcaaatc ttgcccacca
     1021 tgtcgcgcag gatcttggct gacaggaaga gggcgatgat gggctcgacg gagccgttgg
     1081 aatggatctc gaggatgagt ttgtcgaagt tcatgcgttc tttcaccctc tggtgggtga
     1141 cgctgaaatt gaccttgagg atgggcgaat agactgaatc gatgggaatg aagccaactg
     1201 gctttccttc cgggttctga cggtctgcgg aaacataacc gcggccgttg ccgacgatca
     1261 tttccagctt gaaatcgaca tcctcggtga cttccagcaa atagagatcc ttgttgatga
     1321 tccggacgtt tggattgtcc tgtatcttgg cggcggtgac cacgcctttg cccttgtgtt
     1381 caagcaccag attgacctca tccacggaag aggagtagat caccagttgc ttgaggcgca
     1441 ggatcaggtc gatgtaatcc gagttcgtgc cgggaatggg cgtgaattcg tggtgaagac
     1501 cttcgatgcg gacaaagcgc acggccgagc cttggatcga agagaggagc acgcggcgga
     1561 gggtgttgcc gattgtggtc ccgaatcccg gttccagcgg gccgatctcg aacctgccgt
     1621 attggtggga ataggtttcc ctgtcgtaat ccacgttttc gggcatttgc aggggttcca
     1681 agtatagcat aatgactcct tgctggatca gcgattattt ggagtagaac tcaacgatga
     1741 ggcgcaggtc aacagtattg ggaatttccg cagccagagg gatgttcaca aactgcccgc
     1801 gcatgttttc cttgtccaca gtaagccagg ggaagggaga gctggcttcg gaattgttcc
     1861 aggcctcagc aaggggtttg atgcccttgc ttttggggcg gacttcgact atatcaccat
     1921 ctttgagcag gtatgaaggg atgtccactt tcttgccgtt gaccaggaaa tgaccgtggt
     1981 taacgaactg gcgggcctgc atccttgtgg tggcaaagcc gaggcgatag acggtgttgt
     2041 caagtctgcg ctccagcatc tgcaggaggt tgtcgccggt gacgccaccc attttggcgg
     2101 ctttcacgaa gtagttgctg aattgctttt ccagcaggca atagatgttt cttagtttct
     2161 gtttttcgcg caggtggatg aaataatcgc tgggcttgcg gcgcatgttt ctgccatgct
     2221 gaccgggcgg gaatttgcgc cggttcagaa ttctgtcgta tttggccgtg ccaaagatgt
     2281 tttcgccgaa tttccggcag agtttcgctc ttggtcctgt atatcttgcc atgattttcc
     2341 tcgcttatat ccttctggtt ttgggtggac ggcagccgtt gtggggaatg ggggtggcgt
     2401 ctttgatcat ggtgactttg agtcctgaag cgttgaccgc ccggatggct gattccctgc
     2461 cgctaccagg cccgcggacg atcacgccca cgcgggtgag ccccatttcc aagccggttt
     2521 tggagacttc ggtggcggca agctgggccg cgaacggggt ggctttgcgg gaacccttgt
     2581 agccaactct tccgccgctg gaccaggtga ggatgtttcc ggaacggtcg gcgagagaaa
     2641 cgatcgtatt gttaaagctg gagtggacaa acacaatgcc ttcgtcaaac gagaggcgga
     2701 cgcgtttttt cttgattctg gtttttttgg ccatttatct gatcactcct atttcttctt
     2761 ttttatcgcg ccgggtttcg ggcccttgcg agtgcgggca ttggtgtggg tgcgctggcc
     2821 gcgcactggc aagccgcgtt tgtgacgcaa gccgcgatag ctgcctattt ccatcaggcg
     2881 cttgatgttc atggccacct gggtgcggag ggcgccttca accacatatt cgttctgaac
     2941 aatatcgcgg atgatctttt cctcttctat ggagaggtcc tccaccttct tcatctcatc
     3001 gacatttgcc ttgcggcaga tatccctggc gatggagcgg ccgattccgt agatatatgt
     3061 gaggccaatg aacaaacgtt tgtctttggg gatttctata cctgcaatgt gtgccaagtt
     3121 ggtcctcctt aaccctgtct ctgtttgtgt ttggggttgg aactgcagat aacacggatc
     3181 acgccgtggc gtttgattat gcggcagtct ttacagacta cccggactga tgatctcact
     3241 ttcattatcg tctccttgca agagtgcagt gcttcctgca atgta
//
LOCUS       DAOOWR010000191         3263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29476_length_3263_cov_3.482232, whole genome shotgun sequence.
ACCESSION   DAOOWR010000191 DAOOWR010000000
VERSION     DAOOWR010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29476_length_3263_cov_3.482232"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLB85_05270"
     CDS             <1..210
                     /locus_tag="PLB85_05270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU72776.1"
                     /translation="LYRLKNEAEFLDLGITDLIDSGVTLIEWPELAARYLPDRIMNLS
                     LRFVFEGRQRSVEIIAKGRFSDYFV"
     gene            235..1014
                     /locus_tag="PLB85_05275"
     CDS             235..1014
                     /locus_tag="PLB85_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOU72777.1"
                     /translation="MSFNPLWIALVAIVVMAVWEAMGKDKSPHRGWLMAGVCFVLGLA
                     VFGYFHMESRLENDNLQVVGYASKTFESDLVKWTLSMQKNTGVDGLMQTYTSLNRDVS
                     DFKALLLSKGLTAKDINIQPPTSAPHYDNYGNMTGYSVDQMLYVISNDIHKVEEIALD
                     PEFFAERSIVLQQSNLEYLYSKLPELKKEMIGAATADAMDRAKEIVGATKARLGKLSS
                     ARSGVFQITEPYSTDVSDYGYYNTDTRTKSISVTVTTKFRL"
     gene            1486..>3263
                     /locus_tag="PLB85_05280"
     CDS             1486..>3263
                     /locus_tag="PLB85_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU72778.1"
                     /translation="MRKLLIAALLVFVAALWAQGGTLPECYYTYAQISQMLIDHQTQH
                     PDIAKIVQIGFTQQDHLPIYAMRISDNVDQDEEEPALLFVGQVHAEEVLGVQITMNNI
                     AEILANRDQMPYMQWINFLDMWFVPTLNPEGHNVVTANLDTSYRKNKRDNNMNGVFDY
                     SVLVGYDVDGVDINRNFDFNWCHGDTLLQPPTVDQIEPYDYYRGPGEMSESEIQAIKN
                     LCDQYPFVYSICWHSSRRGTLSEKCYVPFNWKNVRPSPDFNFAQTICSGVANQIVNEA
                     GTGTYDAFPNLSRKGAFHDWMYKQYGTFQILIECGTANLQPNEALMQNTVQRCRSAVY
                     WMLNRTLPYSQAVPSSSMLTGNISDAVTGAPLEAEIIVEGFHAPWFVPRTSNPVTGRF
                     YKPLSTGTYNLRFRKKGYWDTVIPNQMVYNNNWTQIQVQMQPREPASLSGRVLSGGAD
                     IAAQIVIGDVFPDTLAVNGHFVYNGFEGEYPVRVYAEGYYPWLGTVELSPGVNNLLLE
                     LSPAEVLFAEDWESGTDGWTIEGPWVLQNELSTAGYAITDSWGGNGFYAVNCDVWIKP
                     THRLTLPSGTSAMLTFDSHLYTEWNF"
BASE COUNT          780 a          918 c          838 g          727 t
ORIGIN      
        1 ctttaccggc tgaaaaatga agccgagttc ctcgatttgg gcataaccga cctcatcgat
       61 tccggcgtaa ccctgatcga atggccagaa ttggcggcga ggtaccttcc ggacagaatt
      121 atgaatcttt ccctgcgctt cgttttcgaa ggccggcaaa ggtcagtgga aataattgcc
      181 aagggccgtt tttcggatta ttttgtttaa caataacgtc atttggaggc tcagatgagt
      241 ttcaaccctc tctggatagc tcttgtcgcc atcgttgtga tggcagtttg ggaagccatg
      301 ggcaaagaca aatccccgca ccggggctgg ctgatggccg gcgtctgttt tgtgctggga
      361 cttgctgtct ttggatattt ccacatggaa agccggctgg aaaacgacaa cctgcaggta
      421 gtgggttatg ccagcaaaac ctttgaaagc gacctcgtga agtggaccct ctccatgcag
      481 aaaaacaccg gcgtggacgg gctgatgcaa acctatacca gcctcaaccg cgatgtgagc
      541 gacttcaagg cgttgctgct ttccaaaggc ctcaccgcca aggacatcaa catccagcca
      601 cctaccagcg caccgcatta cgacaactac ggcaacatga ccggctacag cgtggaccag
      661 atgctctatg ttatcagcaa cgacatacac aaggttgagg aaatcgccct cgatccggag
      721 ttctttgccg aacgctccat cgtgctgcag cagtcgaact tggaatatct ctattccaag
      781 ctgcccgaac tcaagaagga gatgatcggc gccgccacag ccgatgccat ggaccgcgcc
      841 aaagagatcg tgggcgccac caaagccagg ctgggcaagc tcagcagcgc ccgaagcggc
      901 gtgttccaga tcaccgagcc ctactccacc gatgtttcgg actacggcta ttacaacacc
      961 gacacccgca ccaaaagcat atcggtgacc gtcaccacaa aattccgcct ctgatcccct
     1021 ttgatcattg cattatccat acggaattgt tactgatgaa gaccgtaatg attccgtatg
     1081 gttcttttcg tgttgggttg gcatagcctt ccgggctgcc ccctgcttgg gtgcccacac
     1141 ccacttgatt ggaaagagaa ccttaactgc ttggccaccg caatgtcctg ccagtcaggc
     1201 cctgaacgct cgcttccatc cctctcccac gccccgcgca tgatactcgc atcaaatgcg
     1261 ggcattgtgc gggaggcgag tccgggacac tcgacagggc cttaacggcg agctgacatt
     1321 gcttcgcttg ttcgcgggga gccttgcgct ggggtaacgg cagccgcggc aactcctgcg
     1381 caacatgagc catcctgttg atgctgtaat gatggggaga gccgctccta aaatggaata
     1441 agaattgcat taattgaaaa ctaccaaaat gtttggagac caataatgag aaaactgctg
     1501 attgcagcgt tgctcgtctt tgtggccgcg ctctgggccc agggcgggac tttgccggaa
     1561 tgctactaca cctatgctca gatatcgcag atgctgatcg atcatcaaac ccagcatccg
     1621 gacatcgcca aaatagtgca gataggtttc actcagcagg atcatctgcc gatatacgcc
     1681 atgcgcattt ccgacaacgt tgaccaggac gaggaggagc cggcgctgct ctttgtgggg
     1741 caagtacacg cggaggaagt tctgggcgtg cagatcacga tgaacaacat tgccgaaatt
     1801 ctggccaatc gcgaccagat gccctatatg cagtggatca actttctgga catgtggttc
     1861 gtgcccacat taaatcccga ggggcataac gttgtaacag ccaatctcga cacctcatat
     1921 cgcaagaaca agcgggacaa caatatgaac ggggtcttcg attacagtgt cctggtgggt
     1981 tatgacgtcg acggagtcga tattaatcgt aacttcgatt tcaactggtg ccatggcgac
     2041 acgcttttgc aaccgcccac ggttgaccag atcgaacctt atgactatta cagggggccc
     2101 ggagagatga gcgaaagcga aatccaggca ataaaaaacc tctgtgacca atatcccttt
     2161 gtttactcaa tttgttggca ttcctcccgc aggggcaccc tcagcgagaa atgctatgtt
     2221 cctttcaact ggaagaacgt gcgcccctcc ccggacttta attttgccca aacaatttgt
     2281 tccggagtcg ccaaccagat agtcaatgag gctggaacgg gcacttatga tgcgtttccc
     2341 aatctcagcc gcaaaggcgc gtttcacgat tggatgtaca agcagtatgg caccttccag
     2401 attctgatcg aatgcgggac cgcgaatctc caacccaacg aagcgctgat gcaaaacacc
     2461 gtccagcgct gccggagcgc ggtttactgg atgctgaacc gcactttgcc ctattcccag
     2521 gcagttcctt ccagttccat gctcaccgga aacatcagcg atgccgttac cggcgcgccc
     2581 ctggaagccg agataatcgt ggaaggcttt cacgcgccct ggttcgtacc ccgcacttca
     2641 aatcctgtta caggcaggtt ttacaagcca ttgtcaaccg gaacctacaa cctccgcttc
     2701 cgtaaaaagg gctattggga caccgtgatc ccgaaccaga tggtttacaa caacaactgg
     2761 acccagatcc aggtgcagat gcagccgcgc gagccagcca gcctaagcgg cagggtgcta
     2821 agcggcggag ccgacatcgc tgcccagatc gttatcgggg atgttttccc ggacaccctg
     2881 gcggtgaacg gacactttgt ttataatggt tttgagggcg aatatcccgt gcgggtttac
     2941 gccgagggct actatccctg gctgggcaca gtggaacttt ctcccggcgt gaacaacctt
     3001 ctgctggaac tcagccccgc cgaagtgcta tttgctgaag actgggaatc cggcactgac
     3061 ggttggacca tcgaaggtcc gtgggtgctg caaaacgagc tttcaaccgc tggttacgcc
     3121 atcaccgaca gttggggcgg aaacggattc tacgccgtca actgtgatgt ctggataaag
     3181 cccacacatc ggcttacctt gccttctgga acctctgcca tgcttacttt cgattcccat
     3241 ctctacaccg agtggaattt cga
//
LOCUS       DAOOWR010000192         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29923_length_3224_cov_3.659514, whole genome shotgun sequence.
ACCESSION   DAOOWR010000192 DAOOWR010000000
VERSION     DAOOWR010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29923_length_3224_cov_3.659514"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1042
                     /locus_tag="PLB85_05285"
     CDS             152..1042
                     /locus_tag="PLB85_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU72779.1"
                     /translation="MSLVIVGSVALDTIETPHGKVEDALGGSAVYASLAASYFGPTYI
                     VGVVGEDYPARGVELLEAHKVNLDGLETKPGKTFRWSGAYRQWSQADTLSTDLNVFAD
                     FSPQLPHSCRSCRSLLLANIHPQLQLQVLNQIESYKWAACDTMNYWISLCPELLSEVI
                     RKVDIVFINEDEIRQYTGLDSIFAAGRHLLEMGVKAVVIKRGEYGSVTILPDDLFFAP
                     AYPVERIQDPTGAGDSFAGGFMSYLATQDELNKYVIRKAVRFGTVLAAKNVSAFSVDG
                     LVNLEFSELQQKVHQLRNWA"
     gene            1059..2093
                     /gene="purM"
                     /locus_tag="PLB85_05290"
     CDS             1059..2093
                     /gene="purM"
                     /locus_tag="PLB85_05290"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOU72780.1"
                     /translation="MNKDTMDYRGSGVDIKAGEQAVDSIRGIVARTYNANVLSELGSF
                     GGLYRFPKDSYREPVLVSSTDGVGTKLRVAIKARRFDTIGQDLVNHCVNDILVQGALP
                     LFFLDYIGLGKMEPDMIVQIISGMAKACSENGCALIGGEMAEMPGIYQGGDFDLVGTI
                     VGVVDKEHLLPAARIKKGDILIGIPSSGLHTNGYSLARRIVFDRLGLDVADRVPELDA
                     TVAELLLSVHRSYLPMLKPLLRDERLHGLAHITGGGIPGNLKRIIPKGLCGVVSYAEH
                     EIPPLFTWLQTSGGLSRETMLETFNLGLGMIAAVDPAFSTEFISATSSLVIGEIDTSR
                     DPDEKVLIID"
     gene            2568..>3224
                     /locus_tag="PLB85_05295"
     CDS             2568..>3224
                     /locus_tag="PLB85_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOU72781.1"
                     /translation="MRKTALILLILLIGCATFLEAATTGRLAVRVRDNAGKPLEYVNI
                     IVMRGNQRITGGQTDAKGSRILINIPPGMYTVKFSLIGYDSVTYTDVRIQVDQTASLS
                     PVMKKAGVKIASVTVTAEQDVVGKDRTASERQIEMDRMSDVSVSDVAGIMALQAGVSN
                     IGGELHVRGSRANEINFTVDGQSVTDPVDGGSALQVDTDAILDMKVMTGGFPAEYGNA
                     Q"
BASE COUNT          759 a          919 c          890 g          656 t
ORIGIN      
        1 caatcatggt gctggcgctc atcgcaatct tcatcgtgct gaacaggctg aacaaagtgg
       61 gtgagaaaga attgcccagc atcgaataca cagcgcccga cagttcccag gcgccccaaa
      121 ctcagcccca gccggacgga gaagcccttt aatgagcctg gtaatcgtcg gctcggtcgc
      181 cctggacacg atcgagactc cgcacggcaa agttgaagat gccttgggcg gttctgccgt
      241 ctatgcttcc ctagccgcct catattttgg ccccacctac attgtgggtg tcgtgggcga
      301 agattacccc gcacgcgggg tagaactgct tgaggcccac aaggtcaacc tggacggtct
      361 cgaaaccaag cccggaaaaa ccttccgctg gagcggcgct taccgccagt ggagccaggc
      421 ggataccctt agcaccgatc tgaacgtctt cgcggatttt tcgccccagc ttccacactc
      481 ctgcagaagc tgccgcagcc tgcttctggc caatatccac ccccaactgc agttgcaggt
      541 gctgaaccag atcgaaagct acaagtgggc ggcctgcgac accatgaact actggatatc
      601 actgtgtccg gagcttcttt ccgaggtgat ccgcaaggtg gacatcgtct tcatcaatga
      661 ggacgaaatc cgccagtaca caggcctgga cagcatcttt gccgccgggc gccaccttct
      721 ggaaatgggt gtgaaagccg tggtgatcaa acgcggcgaa tacggcagcg taaccatttt
      781 gccagacgat ttgtttttcg ccccggccta tccggtggag cggatccagg accccaccgg
      841 cgccggagac agctttgccg gaggcttcat gagctatcta gccacccagg atgaattgaa
      901 caaatacgtg attcgtaaag ccgtccgttt cggaacggtg ctcgcggcca agaacgtgtc
      961 cgccttcagc gtggatggct tggtcaatct ggagttttcg gaattgcagc aaaaagtcca
     1021 ccagttgcgc aactgggctt gatcgaggca aaaccagcat gaacaaagac actatggact
     1081 accgcggctc gggagtggac atcaaggccg gcgaacaggc cgttgattcc atccggggaa
     1141 ttgtagccag aacctacaac gccaatgtgc tcagtgagtt gggcagtttc ggcggcttgt
     1201 accgctttcc caaggattca taccgcgaac cggtgctggt gtccagcacg gacggcgtgg
     1261 gcaccaaact ccgcgtggcg atcaaagccc ggcgcttcga caccattggt caggacctgg
     1321 taaaccactg cgtaaatgac attctcgtcc agggcgccct gcccttgttt tttctcgatt
     1381 acatcggcct gggcaaaatg gagccggaca tgattgtcca gatcatctct ggcatggcta
     1441 aagcctgttc ggaaaacggc tgcgccctca tcggcggtga gatggcggaa atgccgggca
     1501 tctatcaggg tggcgatttt gacctcgtgg gcaccatcgt gggcgtggtg gacaaagagc
     1561 atctgctgcc cgcagccagg atcaaaaaag gcgacatcct catagggatt cccagcagcg
     1621 ggctgcacac caacggctat tccctggccc gcaggatcgt gtttgaccgc ctcggcctgg
     1681 acgtcgcgga cagagttccg gaactggatg ccacagtcgc ggaactgctg ctcagcgttc
     1741 accgcagtta tctgcccatg ctgaagccgc tgctccgcga tgagcggctg catggcctgg
     1801 cccacatcac aggcggaggc attcccggaa acctaaaacg catcatcccg aaggggctct
     1861 gcggagttgt cagttatgct gaacacgaga tcccgccgct cttcacctgg ctgcaaacct
     1921 ccggcggcct gagccgggaa accatgctgg aaaccttcaa tctgggcctg ggcatgatcg
     1981 cggctgtcga tccggctttc agcacggaat tcatctccgc cacctcgtcg ctggtgatcg
     2041 gagagataga tacatcccgc gacccggatg aaaaagtcct catcattgac tgaccccggc
     2101 ccatcaaacc acttttaccc cctgccgcga atgctgttag gccggtttgg ccaacctgac
     2161 cggccctttg ccctatccaa ttagcctctc acttacttcc cgttgacttc ccgtttgaca
     2221 ggcgggaact cagcgggaaa tgaatgggtt gtgagtagga cagggcaaag ggcctaaccc
     2281 cgggaaaggc agaagcagac agccatccag cccttaatag ggcggattcc tcagaaatat
     2341 ccatagaaac ggaaagacaa agagagggga attttatccg gattttagag cgccaagatt
     2401 aagggtgaaa aacagtcccg caaaacattt cgcgaaaaaa tgcttgacga ttttggctga
     2461 gccagataat cgcatcctac gtagaaatac tgtgttgaga atgaatgtat ttctttacag
     2521 agcgtgaatt aagagtaaaa aagccataca agaagggagg tttttcgatg cgaaaaactg
     2581 cattgatcct gcttatactg ttaatcggtt gcgccacttt tttggaagca gccacgaccg
     2641 gacgcctcgc tgttcgcgtg cgagacaacg caggcaaacc acttgaatac gttaacatca
     2701 ttgtcatgag aggtaaccag cggattaccg gtggacagac cgacgccaag ggaagcagaa
     2761 ttctcatcaa catcccgccc gggatgtaca cagtgaagtt ttctctgatt ggctacgact
     2821 ccgtcaccta caccgatgtc cgcatccagg ttgaccagac tgccagcctc tcgcccgtga
     2881 tgaaaaaagc cggcgtgaaa atcgcctccg tcacggtaac cgcagaacag gacgtggttg
     2941 gtaaagaccg cacggcgtct gaacgccaga tcgaaatgga ccgtatgagc gatgtctctg
     3001 tgagtgacgt ggccgggatc atggctctgc aggccggtgt ttccaacatc ggcggagagc
     3061 ttcacgtccg tggcagtcgc gccaatgaaa tcaacttcac ggttgacggc caatcggtta
     3121 ccgatcccgt ggacggcggc agcgcgctcc aggtggatac ggatgccatc ctcgacatga
     3181 aagtgatgac cggcggtttc cctgctgaat acggaaacgc ccag
//
LOCUS       DAOOWR010000193         3222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_29950_length_3222_cov_3.883486, whole genome shotgun sequence.
ACCESSION   DAOOWR010000193 DAOOWR010000000
VERSION     DAOOWR010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29950_length_3222_cov_3.883486"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2193
                     /gene="mfd"
                     /locus_tag="PLB85_05300"
     CDS             <1..2193
                     /gene="mfd"
                     /locus_tag="PLB85_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU72782.1"
                     /translation="LLADFGSVPDSHIGLLHEGFTLLDCGLALWTDHEIFNRYRRKRY
                     APRFSPGEEIIDYESLSPGDYVVHIDHGIGIFEGLQIIQLDGQATECLTLRYAGDDRV
                     YVPTWQLKLITKYVAEESAAPTLSRIGSAKWQNTKRKATEQIELIAADIVKLYAERSS
                     RVGIAHQPDTDWQRDLEESFIYDDTPDQVRSTNEIKADMESPAPMERLLCGDVGFGKT
                     EVAIRAAFKAVCSGYQVAVLVPTTLLAEQHCRVFRERLAQYPVRVSMLSRFRSNAQIQ
                     DDISGIISGKVDIAIGTHRLLSRDISFPRLGLLIIDEQHRFGVRHKERLRKLQSNVDT
                     LYMSATPIPRTMNMALARLKEISLMQTSPKERLPIRTIITPRDSEVIRDAIRREIDRG
                     GQAFFIHNRVQTIETVAEELRGLMPEVKFAVGHAQMPERILEEVMEAFIKREFQVLIS
                     TTIVENGIDIPNANTILVDRADTFGLAQLYQMRGRVGRSNRRAYAYLLIPRGTTDVAR
                     KRLEALTQYDYLGAGFQVALRDLELRGAGTILGTKQSGIIQSIGFNYYNSLLERAITA
                     VEAGKSISRELDTPPTRRKLTTEIDLYFPPEYITDDEQRLSIYRRLSGFESFADIADF
                     EAELLDRFGPLPEKAGWLLMYFKLNILAKQADLLNCHVRSHTLTMEFKPELLPPREKV
                     LRFSSQVKQELRFDAAKGLRVTISLDKEMAYHDQFEEALRLLELYVQS"
     gene            2301..>3222
                     /locus_tag="PLB85_05305"
     CDS             2301..>3222
                     /locus_tag="PLB85_05305"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU72783.1"
                     /translation="MIEKMKEMLGKVEADYADLRYETKQVARVTLSKKEVRNYGSTTG
                     DGYVLRVLRRGGLATVCFTKPEQADQAVRRAVENADVLSAHQKEKKHIAPAEVVRATV
                     KAPLLEDPRQISLQEKLTLVKHYSDLLFAQPDIANVDLYYNDVYRDKYFVNTEGSEIN
                     EELVTTKLVGAIISQVGELTQTARMAFGGSDGFQRVRNREEEALRQAKIASDLLKAEP
                     VKAGAYNMVLNPDIAGVFTHEAFGHFSEADIIRDLPAMKEKMKLGTKLGTDILNITDD
                     ATMPGKLGYYKYDDEGVAVRKVKLMTAGVLT"
BASE COUNT          735 a         1020 c          845 g          622 t
ORIGIN      
        1 ctcctagctg atttcggctc cgtcccagac agtcacatcg gcctgctgca cgaaggcttc
       61 actttgctgg attgcggtct ggccctttgg accgaccatg agattttcaa ccgctaccgc
      121 cgcaagcgct atgcccccag attctctccc ggggaggaga taatcgatta cgaaagcctc
      181 agtcccggcg actacgttgt ccacattgac cacggcatcg gcatcttcga gggcttgcaa
      241 atcatccagc tcgacggaca ggctaccgaa tgcctaaccc tgcgctacgc cggcgacgac
      301 cgggtttatg ttcccacctg gcagctcaag cttatcacca aatacgtggc ggaggaaagt
      361 gccgccccca ccctcagccg catcggcagc gccaaatggc aaaacaccaa acgcaaggcc
      421 actgagcaaa tcgagctcat cgccgcggac atcgtgaagc tctacgcgga acgcagttcc
      481 cgagtgggaa ttgcccacca gcctgatacc gactggcaga gggacctcga ggaatctttc
      541 atttacgacg acacgccgga ccaggtgcgc tccaccaacg aaatcaaggc tgatatggaa
      601 agccctgccc cgatggagcg tctgctgtgc ggcgacgtgg gtttcggcaa gaccgaggtg
      661 gcgattcgcg ccgcttttaa ggctgtctgc tccggttacc aggtggcagt gctagttccc
      721 accacccttt tggcggaaca gcattgtcgc gttttccgcg agcgcctggc ccaatatcca
      781 gttcgggttt cgatgctgag ccgcttccgt agcaacgcac aaatccaaga tgacatatcc
      841 ggcatcatct ccggaaaggt ggacatcgcc atcggcaccc acagattgct ttccagggac
      901 atcagctttc cccgcctcgg cctgctcatc atcgacgagc agcaccgctt cggggtgcgc
      961 cacaaggaac gcctgcgcaa gctgcaatcc aacgttgaca ccctctatat gagcgccacg
     1021 cccatcccca gaacaatgaa catggccctc gcccgcctca aggaaatttc cctgatgcag
     1081 acttccccca aggaacgtct gcccatccgc accatcatca cccctcggga cagcgaggtc
     1141 atccgcgatg ccatccggcg cgaaattgac cggggcggcc aggctttctt catccacaac
     1201 cgtgttcaga ccattgaaac ggtcgctgaa gagctgcgcg ggctgatgcc tgaggtaaag
     1261 ttcgccgttg gccacgctca gatgcccgaa cgcattctgg aagaggtgat ggaggccttt
     1321 atcaagcgcg aattccaggt gctgatttcc accaccatcg tggaaaacgg catcgacatc
     1381 cccaacgcca acaccatcct tgttgaccga gccgacacct ttggcctcgc ccagctttac
     1441 cagatgcgcg gacgcgttgg ccgctccaac cgtcgggctt acgcctacct gctcatcccc
     1501 cgtggaacca cagacgtggc ccgcaaacgc cttgaagccc tcacccaata cgattatctt
     1561 ggcgccgggt tccaggttgc cctgcgggat ttggaactgc gcggagccgg aaccatcctc
     1621 ggcaccaagc agagcggcat catccaatcc atcggattca actattacaa cagcctcctg
     1681 gagcgcgcca tcaccgccgt tgaggcaggg aaaagtatct ctcgcgaact ggatacccct
     1741 cccacccggc gcaaactcac caccgaaatc gacctctatt ttcctccgga atatatcacc
     1801 gacgacgagc agcgcctctc catctaccgc cgcctgagcg gatttgaaag ctttgccgac
     1861 atcgcagatt ttgaggcgga actgcttgac cgcttcggcc ccctgcccga aaaggctggc
     1921 tggctgctga tgtatttcaa gctcaacatc ctcgcgaaac aggccgacct gctcaactgc
     1981 cacgtccgca gccacaccct cactatggaa ttcaagcccg aactgctgcc cccgcgcgaa
     2041 aaggtgctcc gcttcagctc ccaagttaag caggaactgc gttttgatgc tgccaaaggc
     2101 ctcagagtga ccatcagtct ggataaggaa atggcttacc atgaccagtt cgaagaggcc
     2161 ctgcgccttc ttgagctcta tgtccagtcc tgatttccct tctgccagac agatttggat
     2221 agagggctga atctcccggc cttccgccga gcgtttcagc cgatgaatta aacaaccgct
     2281 tacaaacaca aggagttgcc atgattgaga aaatgaagga gatgctgggc aaggtcgaag
     2341 ccgattacgc cgacctgcgt tacgagacca agcaggtcgc tcgcgtgacg ctcagcaaga
     2401 aagaggtccg taattacggt tccaccaccg gcgacggcta tgtgctgagg gtattgcgcc
     2461 ggggcggcct cgccacggtc tgcttcacca agccggagca ggcggaccag gctgtccgca
     2521 gggctgtgga aaacgctgat gtcctcagcg cgcaccaaaa ggagaagaag catatcgctc
     2581 ctgccgaagt ggtccgcgcg accgtgaaag cccctctgct tgaggacccg cgccagattt
     2641 ccctgcagga aaagctgacc ctggtgaagc actattccga ccttctcttt gcccagccgg
     2701 atatcgccaa cgtggatttg tattataatg acgtctatcg tgacaaatat ttcgtcaaca
     2761 ccgaaggcag tgaaatcaac gaagaactgg tcaccaccaa gcttgtcggt gccatcatca
     2821 gccaggttgg ggaactcact cagaccgcgc gaatggcttt tggcggcagc gacggctttc
     2881 agagggtgcg aaaccgcgaa gaggaggctt tgcggcaggc gaagatcgct tccgatctcc
     2941 tgaaggcgga gcccgtgaaa gccggcgctt acaacatggt gctcaatccc gatatcgcgg
     3001 gagtctttac ccatgaggct ttcggccatt tttccgaagc cgacatcatc cgcgaccttc
     3061 ccgccatgaa ggaaaagatg aagctgggaa cgaaacttgg aacggatatc ctgaacatca
     3121 ccgacgacgc caccatgccc ggcaaactgg gatattacaa atacgacgac gagggagtcg
     3181 ctgtccgcaa ggtgaagctt atgaccgcgg gcgtgctcac cg
//
LOCUS       DAOOWR010000194         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_30049_length_3213_cov_2.899937, whole genome shotgun sequence.
ACCESSION   DAOOWR010000194 DAOOWR010000000
VERSION     DAOOWR010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30049_length_3213_cov_2.899937"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLB85_05310"
     CDS             <1..982
                     /locus_tag="PLB85_05310"
                     /inference="COORDINATES: protein motif:HMM:NF020468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HOU72784.1"
                     /translation="AHRIDFSYPLYGALNLRAIRVQVPYQKMLYTLTCSAEETWYFEQ
                     VEADFEAIITSFKIQRPNQDYLDNYIRTRADLSGEECVIHWQGKAYSYIPGERRKDLF
                     DVEGYNIVRAVPDERGYLLLGKEVMLFLHSQSGEILSSWQNPVSGRNVPVLHVFNDPA
                     NMDLRFTDEYFQLLHMVLPSSLVGDQFVWHNDLFPYYPNALPRREYPLFSQSDTYQAA
                     DLSQYLVKQSDLEDPLLKSVPALYTFTRIYPWLPFMRMGERPGNIIITGRGCKLEGGF
                     EDLPQYLKDYVLTNAPEFARAPDAYSQPNATIWTNFKKMAESGLIEPEQP"
     gene            1017..2501
                     /locus_tag="PLB85_05315"
     CDS             1017..2501
                     /locus_tag="PLB85_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72785.1"
                     /translation="MKTKLVLCLLLLGTALLGAQGLNPYLNLIHSSRCADGNLRLRWT
                     DPTGLGLDTQCWYSPDGAVWQQASSGIYSDQQMQALIPYEFGQNLRYRLRTELSLMNE
                     NLVYMHTPYLNADLFPPQLGQLGQIGTDAVGDFITIYMPHLDLTESWFGATQAKLYSA
                     LANVTNSFPTLNSIFSYNVYLTTISNPDTIMDSLSYAMVYSFYVADLIQPGLYKLRMS
                     DTGIPSFTRIGNVQSMVMDGKLFLSCNLADLTNDPDFGVWPNSVNALVAASVTMNISV
                     NPSTQEFSFGIGDYSTPGILFFEDHNYQVAQNTLPICSDICFDTTEFRFSYNDAEGDF
                     PLVMELELPDGQILEPMPQSYNYSQPVIYSVQLPYLPDSVTLRYSDNAIDVVEDFHIL
                     VSADDPVQVPAAELSCRLPNPLRGGEPVTISLSGLTKDLLSVRVYNLRGQKLGTLFEG
                     NSAQTNLDLPWNGTMNEIGLDSGVYFLRITQGGRTANQRFVITK"
     gene            2520..>3213
                     /gene="obgE"
                     /locus_tag="PLB85_05320"
     CDS             2520..>3213
                     /gene="obgE"
                     /locus_tag="PLB85_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU72786.1"
                     /translation="MFIDYARISVKPGNGGDGVVSFRREKYIPKGGPDGGDGGRGGSV
                     IAVGDENVNTLLDYRYNKIFRAENGKPGAGARKSGAGGANVYLRLPLGTEIFHVGEDG
                     KKLKIADITTHNEEIILARGGRGGKGNCNFATPTDQAPRRATLGKPTKEMELELVLKL
                     MADVGLVGFPNAGKSTLLSALSSARPKIADYEFTTLEPMLGVVRVSDYQSFVMADIPG
                     IIEGAHLGKGLGH"
BASE COUNT          753 a          945 c          799 g          716 t
ORIGIN      
        1 agcccaccgc attgatttca gctatccact ctacggagcc ctgaacctta gggccatccg
       61 ggtccaagtg ccttatcaga agatgcttta caccctaacc tgcagcgccg aggagacttg
      121 gtatttcgag caggtggaag cggatttcga ggccatcatc acctcattta aaatccagcg
      181 ccccaatcag gattacctgg acaactatat ccgcacccgg gccgacctct ctggcgagga
      241 atgcgttatc cactggcagg gaaaagctta cagctatatt cctggcgaac ggcgcaagga
      301 cctcttcgac gtggagggtt acaatattgt gcgcgctgtt ccagacgagc ggggctacct
      361 gctattgggc aaagaggtta tgctatttct gcacagccag agtggcgaga tcctctcctc
      421 ttggcagaac cctgtttcgg gcaggaatgt gcctgtgcta cacgtgttta acgaccccgc
      481 gaatatggat ctacgcttca ccgacgagta tttccaattg ctgcatatgg tgctgccttc
      541 cagcttggtg ggcgatcagt ttgtctggca caatgacctc ttcccctatt atccaaacgc
      601 gcttccccgt cgggaatatc ctctcttttc ccaaagcgac acctatcaag cggcagacct
      661 ttcccagtat ttagttaagc aaagcgatct ggaagatccg ctactaaaga gcgttccagc
      721 cctgtacacc ttcaccagaa tctatccctg gctgcctttc atgcggatgg gcgagcgtcc
      781 gggaaatatc atcatcacgg gccgcggatg caagctggaa ggcggtttcg aagatttacc
      841 ccaatatctt aaagactacg ttttgaccaa cgcgccggaa tttgcgcggg ccccggatgc
      901 ttacagccag cccaacgcca ccatctggac aaactttaaa aagatggccg agtccggcct
      961 gatcgagcct gaacaaccat aactcaaaaa catcttgatt caccaaggaa actgatatga
     1021 aaaccaaact cgttctttgc ctgctgttgc tcgggaccgc cctgctgggc gcccaaggct
     1081 tgaaccctta tctaaacctt atccacagtt cacgttgtgc ggatggcaat ctgcgcctgc
     1141 gctggaccga tcccaccggt ctgggcttgg acacccagtg ctggtacagc cccgatggcg
     1201 ccgtctggca acaagcctcc agcggaatat acagcgatca gcaaatgcag gccctgattc
     1261 cctacgaatt tgggcaaaac ctgcgttacc gcctgcgaac ggagctgagt ctcatgaacg
     1321 agaacctggt ttacatgcac acaccgtatc tgaatgccga tctctttccc cctcaattgg
     1381 ggcagttggg ccaaatcggc actgacgcgg tgggggattt catcaccata tatatgcccc
     1441 acctcgacct cacggaaagt tggtttggcg ccacccaagc caaactctac tcggctttag
     1501 ccaatgttac gaacagcttt ccaaccctca acagcatttt ttcctacaat gtttacctga
     1561 ccaccatttc caacccggat acgattatgg actcactttc ctacgctatg gtctattcct
     1621 tttacgtggc tgacttgatc cagcctggac tctacaaatt aaggatgagc gacaccggca
     1681 ttccctcttt tacccgcatc ggcaacgtcc agagcatggt gatggacgga aagctttttc
     1741 tgagctgcaa tttggccgac ctgaccaatg accctgattt tggcgtctgg cccaattccg
     1801 tgaacgccct cgtggctgcc agcgtcacca tgaacatttc cgtgaatcca agcacccagg
     1861 agttcagctt cggcataggc gattacagca ctcctggcat tctcttcttc gaagaccaca
     1921 actatcaggt ggcgcaaaat accctgccta tctgcagcga tatctgtttt gacacgactg
     1981 agttcagatt cagttataac gacgcggaag gcgattttcc cctcgtgatg gagctggagc
     2041 ttcccgacgg ccaaatcctc gagcccatgc ctcaaagcta caactacagc cagcctgtca
     2101 tatactctgt ccagcttccc tatcttcctg attctgtaac gcttcgctac agcgacaacg
     2161 ccatcgacgt ggtggaggat ttccacatct tggtaagcgc tgatgacccc gtccaggtcc
     2221 ccgctgctga actcagttgc cgcctcccca atccgctccg gggcggagag ccggtaacga
     2281 tcagcctttc agggctcacc aaggatttgc tcagcgttcg ggtttacaat ctgcgcggcc
     2341 agaaactggg caccctcttt gaaggaaaca gcgcgcaaac caatctggac ttgccctgga
     2401 atggcaccat gaacgaaatt gggctggaca gcggtgtcta ttttcttcgg ataacgcagg
     2461 gcggcaggac tgccaaccag cgtttcgtga tcacaaaata aaggataaca tagctttaga
     2521 tgttcatcga ttatgcccgg ataagcgtca aacccggtaa cgggggcgat ggtgttgtga
     2581 gtttccgccg ggagaaatac atccccaagg gcggaccgga tggcggtgac ggtggccgag
     2641 gaggttcagt gatcgccgtt ggcgatgaga acgttaatac cctgctcgac taccgctaca
     2701 acaagatctt ccgcgccgaa aacggcaaac ccggcgccgg agccagaaaa tccggtgctg
     2761 gcggagccaa tgtctatctg cgcctgcctt tgggcaccga gatcttccac gtgggtgaag
     2821 acggcaaaaa gctgaaaatc gccgacatca ccacccacaa cgaagagata atcctggcca
     2881 ggggcggcag aggcggcaag ggcaattgca atttcgccac ccccacagac caggccccgc
     2941 gacgcgccac cctgggcaaa cccacaaagg aaatggagct ggagcttgtg ctcaaactca
     3001 tggcggatgt gggtttggtt ggatttccca atgctggtaa atcaaccctc ctgagcgcgc
     3061 tctcctccgc ccggcccaag atagcggatt acgaattcac caccctggaa cccatgctgg
     3121 gcgtggtccg cgttagcgat taccagtctt ttgtgatggc agatattccc ggcatcatcg
     3181 aaggcgcgca tttgggcaaa gggctcggac acc
//
LOCUS       DAOOWR010000195         3166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_30607_length_3166_cov_3.899711, whole genome shotgun sequence.
ACCESSION   DAOOWR010000195 DAOOWR010000000
VERSION     DAOOWR010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30607_length_3166_cov_3.899711"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLB85_05325"
     CDS             <1..750
                     /locus_tag="PLB85_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOU72787.1"
                     /translation="EPEVMARAAEFCLSYQPDFMDINMGCPVKKVVKRGAGSALMRDP
                     ARAEAIVKEVSAVLKGRCLLGVKFRSGWNSGSLNYLEFGQMLEEAGADFLCLHARTQS
                     QMFSGQADWSHIAQLKGKLNVPLIGNGDIHSPEDAAKMLAVTNCDSVMIGRGALGRPW
                     IFAQISQLWEGGDYRPVTRELLLQTIFSHIDLALRCKPERVVYKEMRSQLCFYTKGLA
                     GSAELRQKINRAESTAEIKELVLASEAFRLL"
     gene            complement(759..1160)
                     /locus_tag="PLB85_05330"
     CDS             complement(759..1160)
                     /locus_tag="PLB85_05330"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU72788.1"
                     /translation="MSRQTLLLIKPNAFRNRHVGHIISILEEAGFNLLRIKDIQFTPE
                     SAAVFYDIHRGKEFFDRLVEFMCSAPSVALLLEKENAVEELRELIGDADPEKRRPGTI
                     RDLYAEGVTENAVHASDSEASAERETRLIFG"
     gene            complement(1157..1480)
                     /locus_tag="PLB85_05335"
     CDS             complement(1157..1480)
                     /locus_tag="PLB85_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72789.1"
                     /translation="MTNYDDFQINYENLLKLGYAVMDIRYKEYDICNDSQKLVIARVD
                     TDRDEFYQDMLKQYTSVEFKPGEIYEIWTEILMHKVKMSRVLNRDISIKVAALDYIET
                     VYRAR"
     gene            1676..>3166
                     /gene="uvrB"
                     /locus_tag="PLB85_05340"
     CDS             1676..>3166
                     /gene="uvrB"
                     /locus_tag="PLB85_05340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU72790.1"
                     /translation="MGIFKLQTDYLPSGDQPEAIAELEAGIRAGKRFQVLLGVTGSGK
                     TFTIANVISRLDRPVLVLSHNKTLAAQLYGELKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDADINAQIEKLRLRATMSLMERRDVIIVASVSCIYGLGVPEEYREALIRL
                     ETGMKMDRDELLAKLVAAHYSRNDIAFERGAFRVRGDIVDIYPAYLEHCLRVEFFGDE
                     ICRLEKLHPISYQSLGEVTQYPVYPAIHFIMSQEKLNQALSSIENEMNERVAWYLANQ
                     RYVEADRLQSRTKFDLEMLAELGYCSGIENYSRHLTGAKPGEPPNCLLDYFPEDFLFI
                     IDESHVTIPQVHGMFGGDYTRKKNLVDYGFRLPSAFDNRPLRFEEFESYMRSVIFVSA
                     TPADYELELTQGEIVEQVIRPTGLLDPEIEIKPIRNQVDDLIAQIRERLAKNHKVLVM
                     TLTKRMSEDLSVYLQNAGIKAKYLHSDIDSIERAQIIRSLRLGEYDV"
BASE COUNT          746 a          924 c          831 g          665 t
ORIGIN      
        1 gaaccggagg tgatggcccg ggcggcggaa ttctgcctca gctaccagcc cgatttcatg
       61 gatatcaata tgggctgccc ggttaaaaaa gtggtgaaac gcggagcggg ttctgccctg
      121 atgcgtgacc ccgcgagggc ggaagcgatt gtgaaagagg tgagcgctgt gttgaaaggc
      181 agatgcctgc tgggagtgaa attccgcagc ggctggaaca gcggaagcct caactacctg
      241 gaattcggac agatgctgga agaggccgga gcggattttc tttgcctcca tgcccgcacc
      301 cagtcccaga tgttctccgg acaggctgat tggagccaca tcgctcaact gaaggggaaa
      361 ctgaatgtcc ccctgatcgg caatggcgat atccacagcc ccgaagacgc cgccaaaatg
      421 ctggcagtga caaattgcga ttccgtgatg atcggacgcg gagccctcgg caggccctgg
      481 attttcgccc agatcagcca gctttgggaa ggcggcgatt accgccctgt aacccgcgaa
      541 ctccttttgc aaaccatttt cagccacatc gaccttgccc tccgctgcaa accggaaagg
      601 gtggtgtata aggaaatgcg ttcccaactc tgcttttaca caaaaggatt ggcgggcagc
      661 gcggaattgc gccagaagat caaccgggca gaaagcacgg ccgaaatcaa agagctggtg
      721 ctcgccagcg aagccttcag acttttatag aaaggcggtc agccgaaaat cagccttgtt
      781 tcgcgttccg cgctggcctc gctgtccgag gcgtggacag cgttttcagt caccccttcg
      841 gcatagaggt cgcggattgt tcccggcctg cgtttctccg ggtccgcgtc tccgataagc
      901 tcacgcagtt cctccaccgc gttttccttt tccaaaagca gggccacgct gggcgcggaa
      961 cacatgaact ccaccaggcg gtcaaagaac tccttgcccc tgtggatgtc gtaaaataca
     1021 gccgcggatt caggcgtgaa ctggatatcc ttgatccgca gcaagttgaa acccgcctcc
     1081 tccaggatgg agatgatgtg cccaacatgg cggttcctga aggcgttggg cttgatcagc
     1141 agcagggttt gccggctcat ctggcgcgat agactgtctc gatgtaatcc agggcggcca
     1201 ctttgatgga gatgtcgcgg ttgagcactc tgctcatttt cactttgtgc atcaggattt
     1261 ccgtccagat ctcatagatt tcgccgggct tgaattccac cgatgtgtat tgtttcagca
     1321 tatcctggta aaattcgtcc cggtcggtat cgaccctggc gatcacgagc ttctggctgt
     1381 cgttgcagat gtcgtattcc ttgtagcgga tgtccataac cgcgtaaccc agcttgagca
     1441 ggttctcgta gttgatctga aaatcatcgt aattggtcat tttatctctc cagtgttaat
     1501 ttactcgaca aaaacagggg tcctgctaat ctggtgtcca tgctttcggg gaaaagctaa
     1561 aagtcaagca aaaactactg ccggaaacgc ccggaaaagc ggcgggaagg cctcaaagcc
     1621 ttgatccgcc aagcactgag ccattttaag ccatgccaaa atgaggacct aagagatggg
     1681 aatctttaaa ctacagaccg attacctgcc cagcggagac cagccggaag ccatcgccga
     1741 actcgaagcc ggaatccgcg ccggaaaacg ctttcaggtg cttctgggcg tcaccggaag
     1801 cggcaagacc ttcactatcg cgaacgtgat ttcccggctt gaccggcccg tgctggtgct
     1861 atcccacaac aaaaccctcg ctgcgcagct ctacggcgaa ctgaagcagc tttttccgga
     1921 aaacgcagtt gaatacttca tcagttatta cgattattac cagcccgaag cctacatccc
     1981 cggcaaagac atctacatcg aaaaagacgc cgacatcaat gcccagatag agaaactgcg
     2041 gctccgggcc accatgagcc tgatggagcg gcgcgacgtt atcatagtgg cctcggtttc
     2101 atgtatttac ggacttggcg tgcctgagga atacagggag gccctcatcc gtctggaaac
     2161 agggatgaag atggaccggg atgaacttct cgccaaactg gtcgcggccc actacagccg
     2221 caacgacatc gccttcgagc gaggcgcctt cagggttcgg ggcgacattg tggatattta
     2281 tccagcctat ctggagcatt gcctcagggt ggaattcttt ggcgacgaaa tatgccgcct
     2341 ggagaagctg catcccatat cctaccagag tttgggcgaa gttacacaat atcctgttta
     2401 tcccgccatc catttcatca tgagccagga aaagctgaac caggccctta gctccatcga
     2461 gaacgagatg aacgaacgcg tggcctggta tctggccaac cagcgctatg tcgaggcaga
     2521 ccggctgcaa tcccgcacca aattcgacct tgagatgctg gccgagcttg gctactgctc
     2581 cggcatcgaa aactactccc gccacctcac cggcgccaaa cccggcgagc cgccaaactg
     2641 ccttctggat tactttcccg aggatttcct cttcatcatc gacgaatccc acgtcaccat
     2701 tccccaggtg cacggaatgt tcggcggcga ctacacccgc aagaaaaacc tggtcgatta
     2761 cggcttccgg ctgccctccg ccttcgacaa ccgtcccctc cgtttcgagg aatttgaaag
     2821 ctatatgcgc agcgtcattt tcgtttccgc caccccggcg gattacgaac tggaactgac
     2881 ccagggcgaa attgtggagc aggttatccg ccccaccggc ctgctggacc ccgaaattga
     2941 gatcaaaccc atccgcaacc aggttgacga cctcatcgcc caaatccgcg aacgcctggc
     3001 caaaaaccac aaggtgctgg tcatgaccct caccaaacgt atgagcgaag acctcagcgt
     3061 ttacctccaa aacgccggga taaaggccaa atacctgcac agcgacatcg attccatcga
     3121 acgcgcccag atcatccgct ccctacgcct cggcgaatac gacgtt
//
LOCUS       DAOOWR010000196         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_30640_length_3164_cov_4.589900, whole genome shotgun sequence.
ACCESSION   DAOOWR010000196 DAOOWR010000000
VERSION     DAOOWR010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30640_length_3164_cov_4.589900"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLB85_05345"
     CDS             <1..435
                     /locus_tag="PLB85_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72791.1"
                     /translation="VPFLLNSWHSNNFNYQVEHCLTVCAFDGVNESPRTNEVLVSHVS
                     VSDPGAVPPTLVVGPNPASIGAGGGVEVSIKSSAPLNGRLGVYNLRGQLVETVPVASE
                     GDFAWHWGLLDAAGERCASGIYLFRAELEGQPTLQKRLVLIR"
     gene            complement(817..2622)
                     /locus_tag="PLB85_05350"
     CDS             complement(817..2622)
                     /locus_tag="PLB85_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72792.1"
                     /translation="MDLRKLILTALALLFALRAAAVTIEELQDLFYQIEDAEQFSREA
                     AGFIEASEDLADLDHAFNLWESFAPDDCGEWLEARAAEASDEPKYQYLLLNLTEDMQS
                     KISKAKELVSAHPDFAGGYRALLLTFVVNFSPEELDDPESPFYQQLRESLPLLAHFGE
                     NFNGDELFLMAQVYYLVQTGDPEAAKLPFRQALEAEEDWIDEMGLGYMFTPDKYHPLM
                     AYQIELLRPKDADPYTKYRIAELAGDLVDYYFDQKQDYDAVIGFFGAEPWYWENQYVI
                     FALAQSYLVKDQPEKAVNLLNGNGDLPSALLFQDSWLAFDRSEAASAYARVLEPVSAD
                     PVNAYLLARSLSDNGEKLARAHALIASHPKEEHGYSLAAEVYLDYFSKYKADDPQIAV
                     MEASLKNDTQILRNYYFRFPDNNLATVCYYLVNVFERDENKSMRAFQELHKAGLGEMT
                     SNVFARFTLDHCDARFLLRIKEYELRQNEAYGELSEAETAAQTAKEFCVTLSENELFE
                     DLIEEVAKHPEWLDDTETQYMVLNAYYMRDDIASTIATLRLMVDRGTIGTTMLNGLDD
                     PDLTGHADWQALLDFAATRPDPDAEALGNTTEDER"
     gene            complement(2729..>3164)
                     /locus_tag="PLB85_05355"
     CDS             complement(2729..>3164)
                     /locus_tag="PLB85_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU72793.1"
                     /translation="QQRIDGMSVESSSRHWQAIFCGGYTVLAAETEGRVVGFAAGGKK
                     FEYQNCETGLANACDCELEALYLLKKFHGRGMGKALFHRFANLMRQEKKHVMTVWVAE
                     KNPSTGFYEAMGGELVDRMALVVCKVPVPAICYRFTLGIQPV"
BASE COUNT          656 a          907 c          878 g          723 t
ORIGIN      
        1 gtgcctttcc tgcttaatag ctggcactcg aacaacttca attaccaggt tgaacactgc
       61 ctcactgtct gcgctttcga cggcgtgaac gaaagcccgc gcaccaatga ggtcttggtc
      121 tctcacgttt ccgtcagcga ccccggggca gtgcccccaa cgcttgtggt cggccccaat
      181 cccgcttcca tcggcgcagg cggcggggtg gaggtcagca tcaagagttc cgctccgctg
      241 aacggcaggc tgggcgttta caatctgcgc ggacaactgg ttgaaacggt gccggtggcc
      301 tcggaaggtg atttcgcctg gcactggggc ctgcttgacg ccgctgggga gcgctgcgct
      361 tccggtatct atttattcag ggcggagctg gaaggccagc ccaccctgca aaaaaggctc
      421 gtcctgatca gataatccct gcgagtatca aaaagcccgt agggcgacat aacgatagcg
      481 gcgaggggtt acgttcgctg cgctcactac acaccctcgc tatgatctgt cgccctccgg
      541 gctatccact cagttggaaa gagcgctgta actgcttacc cactaccagg ccctgtcagc
      601 caggtcctta gctccctcaa ccatccctct cccatgcctc ccgtacaata ctcgcatcaa
      661 atgcgggcat tatgcgggag gcgtgaccgg agcatgggga aaggccttaa cggccaggca
      721 gcatcgtttc gcttttcccc ggggataccg gagaaaggat tacgcaagtc gctcctgctc
      781 tttggaaatg agccttattg tcgggtataa aagtttttac cgctcgtctt cggtggtgtt
      841 tcccagggct tcggcatccg gatcgggccg ggtggcagcg aaatccagca gcgcctgcca
      901 gtccgcgtgc ccggtcaggt cggggtcgtc caggccgttg agcatggtgg ttccgatggt
      961 gcctctatcc accatcaggc gcagggtggc gatggtgctg gcgatgtcgt cccgcatata
     1021 ataggcgttc aaaaccatgt actgggtttc cgtatcgtcc aaccattcgg ggtgtttggc
     1081 cacttcctca atcaaatcct cgaagagctc gttttcgctg agggtgacgc agaattcttt
     1141 tgccgtctgg gcggcggttt cggcttcgct gagttcgcca taagcctcgt tttgccgcag
     1201 ctcatattcc ttgatcctga gcaggaaccg ggcgtcgcag tggtcgaggg tgaacctggc
     1261 aaaaacgttg cttgtcatct cacccagacc agctttgtgg agttcctgaa atgccctcat
     1321 ggacttgttt tcatcgcgtt cgaacacgtt cacgagatag tagcagaccg tggccagatt
     1381 gttgtccgga aagcggaaat agtagttgcg caggatttgg gtgtcgtttt tgagggaggc
     1441 ctccatcacg gcaatctggg gatcgtcggc cttatatttg ctgaaatagt ccagatagac
     1501 ctccgcggca aggctgtaac cgtgttcttc cttgggatgc gaggcgatca gggcgtgggc
     1561 gcgggctagt ttttcaccgt tgtcggaaag ggagcgagcc agcagatagg cgtttacggg
     1621 gtccgcggaa acgggttcca gcacgcgggc gtaagcgctg gcagcctcgc tgcggtcaaa
     1681 ggccagccag gaatcctgaa agagcagcgc ggagggcagg tcaccgtttc cattcagcag
     1741 gttcacagcc ttttccggct ggtccttgac caagtaactc tgcgcgaggg caaagatcac
     1801 atactggttt tcccaatacc aaggctccgc gccaaaaaaa ccgataacag cgtcgtagtc
     1861 ctgcttctgg tcaaagtaat aatccaccag gtctccggcc agttcggcga tccggtattt
     1921 ggtgtagggg tccgcgtctt tgggacgcaa aagctcaatt tgataggcca ttagggggtg
     1981 atatttgtcc ggagtgaaca tgtatcccaa gcccatttcg tcgatccagt cctcctccgc
     2041 ctccagggcc tgccggaaag gaagctttgc cgcttcagga tcaccagttt gcaccagata
     2101 gtaaacctgg gccatcaaga agagttcgtc cccattaaaa ttttccccga aatgggccag
     2161 caggggcagg ctttcccgca actgctgata gaatgggctt tcggggtcat caagctcttc
     2221 gggggaaaaa ttcacaacaa aggtgagcag cagggcacgg taacccccgg caaaatccgg
     2281 atgggcggaa accagctctt tcgccttgct gattttggat tgcatgtcct ctgtcaggtt
     2341 cagcagcagg tattggtact tgggttcgtc agaagcctcc gcagccctgg cctccagcca
     2401 ctccccgcaa tcatccgggg cgaagctttc ccagaggttg aaagcgtggt ccagatcggc
     2461 aaggtcttcg ctggcctcga tgaagccggc agcctcgcgg gaaaactgtt ccgcatcctc
     2521 tatctgatag aagaggtcct gcagttcttc gatcgtgact gccgccgccc gcagggcgaa
     2581 taacagggcc agagcggtca gtattagctt gcgcaaatcc atttcatcct cccgaatgac
     2641 ttcgtgtatg aaaacgcaga aacccccgca ggcagagaaa tgtcaagtga aatctgccgg
     2701 atttgttggc tggctcccac tggaaaattc agacaggctg aatcccgagt gtgaatctgt
     2761 agcaaatcgc cggaacaggc actttgcaga ccacaagcgc cattctgtcc accaattccc
     2821 cacccatggc ctcatagaat cctgttgacg ggttcttttc cgccacccac acggtcatga
     2881 catgcttctt ttcctgcctc ataaggttgg cgaagcgatg gaacagggct tttcccatcc
     2941 ctctgccatg aaacttcttc agcagataca atgcctccag ttcgcagtcg caggcattgg
     3001 ccagcccggt ttcacaattc tggtattcga atttttttcc gccagcggca aaacctacga
     3061 cccggccttc ggtttccgcc gccagaaccg tgtatccgcc gcaaaagatc gcctgccaat
     3121 gcctggagct gctctcgacg ctcatgccat caatccgctg ttgc
//
LOCUS       DAOOWR010000197         3156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_30757_length_3156_cov_4.071267, whole genome shotgun sequence.
ACCESSION   DAOOWR010000197 DAOOWR010000000
VERSION     DAOOWR010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30757_length_3156_cov_4.071267"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1833)
                     /locus_tag="PLB85_05360"
     CDS             complement(259..1833)
                     /locus_tag="PLB85_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOU72794.1"
                     /translation="MKRAKQMKILWVDDEIDLLQPFVLFLEERNYLVDTCNNGNDAID
                     KALENKYDLVIMDEMMPGLDGLATLQEIKRVNPSLPIIMVTKSEEEGLMDKAIASQIA
                     DYLIKPINPNQIIMAIKKIFQADQIRANQIGEQYTRYIAQLNQRLFDNPGWEDWASIY
                     REMAYWEIQIDEVNDDGLRQTHFLEKRNCNTEFTNYVERNYRDWLQTDDRPNLSFDLV
                     SQYVAPHFETRVPVYFIIIDCMRLDQYLSIQPYIKELFDEELDLYYSILPTATPYSRN
                     SIFSGLLPQDIAKRFPEYWVGSGEMDNSRNRNEHQLLDEHIAELGYNLDPSSKYVKIF
                     NMDEGNFVLRKIETWNKEKLVVLVYNFLDLLAHHRSRDQILQETIPHEEGLRAFTKHW
                     FLHSALYEALKLIAKQDAICVISTDHGSIKVNRATQVIGDRDTSITVRYKEGKNLSAN
                     DRHAIYVKKPSEYGLPIKSIVDNFIFAKDDYYFVYPNSYHQYQRQYNGTFQHGGVSME
                     EMILPVATCRSKRLKI"
     gene            complement(1830..2453)
                     /locus_tag="PLB85_05365"
     CDS             complement(1830..2453)
                     /locus_tag="PLB85_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72795.1"
                     /translation="MIAKYEHLETMTPAEKFSAVANLKEKLEENFVSLGQLLSEIKRS
                     KIFRMKGYESFKDFVEAEYSLSGSLAGKLAAVFDVFIDEMDVDEGTIREIGFDRLQMI
                     QPLVRKADWALRDEWVQKAEEMPTKDLRNHIKELKKEEKEKEIDPKKVFIDQYLERMT
                     GILNCSRTELNYKLALFFQDADPEHVKNVVRERQRSFENELNKEESP"
     gene            complement(2466..>3156)
                     /gene="panB"
                     /locus_tag="PLB85_05370"
     CDS             complement(2466..>3156)
                     /gene="panB"
                     /locus_tag="PLB85_05370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU72796.1"
                     /translation="VGDSLGMVVLGYENTLKVTMDDMVSHTAAVRRGAPQSFVIADLP
                     YMSYHLGLDDTKANAAALIIEGGADAVKLEGGSEERLEAIAAILECEIPVCAHIGLTP
                     QSVRRFGGFRVQGKSPEAHETLLRQALALEKAGVFMLVLEGIPEGLGKEISKNVSIPT
                     IGIGAGRYTDGQVLVYHDLLGHSDLQPKFVRSYASLDETITSALNKYCAEVRSGAFPG
                     EENVYYPISES"
BASE COUNT          627 a          754 c          923 g          852 t
ORIGIN      
        1 gccaggtccc gggtgtcctg ctctgaatca agtttgatgg ttttgggttt ggtcactggt
       61 ggctccatat atcattttga aacataagaa agcctgttgg cggtgaagga tccaatcgcg
      121 gttttcttga ggggcggaga aggttttgac cgcatatttt cagaaaagaa agtcccaaaa
      181 gagcctgcgg gcatcttttg ggacaatcat caggcttaat cttgaagaca gggtgattct
      241 cagcgggacg cgcgtcaatc agattttaag gcgtttgctc cggcaggttg ccacaggcag
      301 gatcatttcc tccatggaaa cgccgccgtg ctggaaggtg ccgttgtatt gcctttgata
      361 ttggtggtag gaattgggat agacgaaata atagtcgtcc ttggcaaaga tgaagttgtc
      421 cactatgctc ttgatcggca gtccgtattc ggagggtttt ttcacataga tggcgtggcg
      481 gtcgttggcg ctgaggttct tgccttcctt gtagcgcacg gtgatggagg tgtcgcggtc
      541 tccgataacc tgggtggcgc ggttcacctt gatggagccg tggtcggtgc tgataacgca
      601 gatggcatcc tgtttggcga tcagcttcaa tgcctcgtag agcgcggaat gcaggaacca
      661 gtgtttggtg aaggctcgca ggccttcctc gtgggggatg gtttcctgga ggatttggtc
      721 gcgggaacgg tggtgggcca gcaggtcgag gaagttgtaa accagcacca ccagtttttc
      781 cttgttccag gtctcgatct tgcgaagcac aaagttgcct tcatccatat tgaagatctt
      841 tacgtatttg gagctgggat caaggttgta gccaagttcg gcgatatgct catcgagcag
      901 ttggtgttcg ttgcggttgc gggaattgtc catttcgccg ctgcccaccc agtattcagg
      961 gaagcgtttg gcgatgtcct gcggcagcag gccgctgaaa atggaattgc ggctgtaggg
     1021 cgtggctgtt ggcaggatgg agtaatagag gtcaagctct tcgtcgaaga gttccttgat
     1081 gtatggctgg atggacagat actggtcgag gcgcatgcaa tctatgatga tgaaatagac
     1141 cggcacgcgg gtttcaaagt ggggcgcgac gtactgggaa accaggtcga atgaaagatt
     1201 gggacggtca tccgtttgca gccagtcgcg gtaattgcgt tccacgtagt tggtgaattc
     1261 ggtgttgcaa ttgcgctttt ccaaaaaatg ggtctggcgg aggccgtcgt cgttaacttc
     1321 gtcgatctgt atctcccaat aggccatttc gcggtagatg ctggcccaat cttcccagcc
     1381 ggggttgtcg aagaggcgct ggttgagctg ggcgatgtaa cgggtgtact gttcgccgat
     1441 ttggttggcg cgtatctggt cggcttggaa gatctttttg atggccatga tgatctggtt
     1501 ggggttgatg ggcttgatca ggtaatcggc tatctgcgag gcgattgcct tgtccatcaa
     1561 tccttcttcc tcgcttttgg tgaccataat gatcgggagg gagggattta cacgtttgat
     1621 ctcctgcagg gtggcgagcc catccaggcc gggcatcatc tcgtccatga tcaccagatc
     1681 gtatttattt tcaagcgcct tgtcgatggc gtcattacca ttgttgcagg tatccaccag
     1741 gtaattgcgt tcttccagaa aaagcacgaa gggctgcaga agatcgattt cgtcatcgac
     1801 ccagaggatt ttcatttgct tggcacgttt catggggatt cctccttgtt gagttcattt
     1861 tcgaaactgc gctggcgttc gcggaccacg tttttcacgt gttcggggtc ggcgtcctga
     1921 aaaaacaggg ccagcttgta gttgagttca gttctggagc agttgagaat gccggtcatc
     1981 cgttccagat actggtcgat gaacactttt ttgggatcaa tttccttttc cttttcttcc
     2041 tttttgagct ctttgatgtg attgcgcagg tctttggtgg gcatttcctc agctttttgc
     2101 acccattcgt cgcgcaaggc ccagtcagct ttccgcacca ggggctggat catctggaga
     2161 cggtcgaaac cgatttcgcg gatggtgccc tcgtcaacgt ccatctcatc gataaaaacg
     2221 tcaaacactg ccgccagctt gccggccagg gaaccgctga ggctgtattc ggcttccaca
     2281 aagtctttga aagactcgta tcctttcatg cggaagatct tgctgcgctt gatttccgat
     2341 aacaactggc cgaggctgac gaagttttcc tccagctttt ccttgaggtt tgccacggcg
     2401 gagaattttt cggccggggt catggtttca agatgttcat attttgcaat catctatttt
     2461 agtccttatg attctgaaat tgggtagtaa acgttttcct cgccggggaa ggcgccggaa
     2521 cgcacctcgg cgcaatactt attcagggct gaagtgatgg tctcgtccag gctggcatag
     2581 gatcgaacaa actttggctg caggtcagaa tggcccagca ggtcgtgata aacaagcacc
     2641 tggccgtcgg tgtaacgtcc cgcgccaatg ccaatggtgg ggatggatac gtttttggat
     2701 atttctttgc ccaagccttc agggatgcct tccagcacca gcatgaagac gcctgccttt
     2761 tccagggcca gggcctggcg cagcaaggtt tcatgggctt cgggggattt gccctgcacc
     2821 ctgaaacctc cgaagcggcg cacgctttgc ggagttaggc caatgtgggc acagacgggg
     2881 atttcgcatt ccaggatggc agctatggct tccaaccgct cttccgaacc cccttccagc
     2941 ttcacggcat ccgcgccgcc ttcaatgatc agggcggcag cgttggcctt ggtgtcgtcc
     3001 aatcccaaat ggtagctcat gtagggaagg tccgcgatca caaaggattg cggcgctccc
     3061 cggcgcactg cggcggtgtg gctgaccatg tcgtccatag ttaccttcag cgtgttttca
     3121 tagcccagaa ccaccattcc caggctgtcg ccaacc
//
LOCUS       DAOOWR010000198         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_30999_length_3138_cov_3.642232, whole genome shotgun sequence.
ACCESSION   DAOOWR010000198 DAOOWR010000000
VERSION     DAOOWR010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30999_length_3138_cov_3.642232"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1032)
                     /locus_tag="PLB85_05375"
     CDS             complement(55..1032)
                     /locus_tag="PLB85_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOU72797.1"
                     /translation="MNNKRIGFTTSFPVEVVFAAGHTPVDLNNIFVEGDSATHVRNAE
                     LKGFPRTICAWIKGNYDAALSADLDEVVGIVQGDCSNGASLSAMLAEAGLPVWHFSFP
                     LERTWEALDAEIIKLEAHFEVSRDEVMKVKRRLDAIRAKLKTLDEWTWRERLVTGKEN
                     HIWLVNSSDFWGDPDRFEEELDSFLRDAANRDPIPYGLRCAYLGVPPIYRDIYDSIVQ
                     QGADVLYNEVQRQFAMPSLLPDIVDQYLAYTYPYSVFERLEDILPQLSQRRIDVVISY
                     TQSFCHLQIDNILLKKHIDLPFLTLEGDQPEALDNRTLLRLESFFEVHG"
     gene            complement(1022..2932)
                     /locus_tag="PLB85_05380"
     CDS             complement(1022..2932)
                     /locus_tag="PLB85_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU72798.1"
                     /translation="MSLIRARDLSLEFGGNYILDQVDCSLEQNSRVGLIGANGSGKTT
                     LIRLFLGLLQPSSGEVLRARNCRVAWLPQDFKLEPDITLLEHVRASRADLISLGDEID
                     RLSRELAATHSAETEARLNLCVERYTSLGGYEFENELKYVLTSLNLPPDTWNKCCGDF
                     SGGEQTRICLAAILLLEHELLILDEPTNHLDLAMMAWLERYLLKQAKPFLIVSHDRQF
                     LDNTVSSIWCLREGQISVTKGNYSSFKAADEIARLSQERQYERQQKFIAEIQAYIQKF
                     IAGTRTSSARSRQKMLERMEIVKKPGQQKQLRLPVAAGKRSGNDVYTLTNLCFGIPPE
                     LELARELDLKAHYRDRICVIGPNGCGKTTLLNILLGEHEIMGGSLKTGASLDIGYYDQ
                     HQVLLDDSLTVMETIWQLVPQAPQGYVLGWLARFGFRGDNVDKKVHVLSGGERSRLYL
                     CQMIHENPNLMILDEPTNHLDIDMTDALLEALKAFSGTIIFVSHDRWFLSQLATKFWV
                     FRKKTSPEGIYTTVEEPDCDWQRAIELSFEEPELTKAPPPPRERKKKVNPWYLEQIHL
                     SIEQGGQRLGELQNELESIHIELSRSETYAEPGRLSTLQTRMAELESEIGTLESQIDE
                     WEEQYLRQSYEQ"
BASE COUNT          658 a          856 c          890 g          734 t
ORIGIN      
        1 aaacggcgct cacagcggaa tcgataccac cgctcatggc cagcagaacc cgcttcatcc
       61 gtgcacctcg aaaaaactct ccaaacgcag cagagtcctg ttatccagcg cttccggctg
      121 atctccttcg agagtgagga agggcaggtc gatgtgcttt tttagcagaa tgttgtcgat
      181 ctgcagatgg caaaaagact gggtgtagct aatcaccacg tcgatgcggc gctggctgag
      241 ctggggcagg atatcctcca gacgctcgaa aacgctgtag ggatatgtgt aagcgaggta
      301 ttggtccacg atgtccggca atagagatgg catcgcgaac tggcgctgca cctcattgta
      361 gagcacatcc gcgccctgtt gaacgatgct gtcatagata tcacggtaga tgggaggcac
      421 gccaagatag gcacagcgca gcccgtaggg tatgggatcg cggttggcgg cgtcgcgcag
      481 aaaggaatcg agctcttctt cgaagcgatc aggatcgccc caaaaatcag acgaattcac
      541 cagccagata tggttttcct tgccggtcac aaggcgttcg cgccaggtcc attcatccag
      601 cgttttaagc ttggcgcgga tggcatccag gcgtcttttc accttcatca cctcatccct
      661 gctaacttcg aaatgcgctt ccagtttgat tatctcggca tccaacgctt cccaggtgcg
      721 ctccagcgga aaggaaaaat gccagaccgg caatcctgct tctgccagca tcgcgcttag
      781 cgaagcgcca tttgagcaat ctccctgcac aatgccgaca acttcatcaa ggtcagcgct
      841 gagggcagcg tcgtaattac cctttatcca ggcacagatg gtgcgcggaa agcctttcag
      901 ctcagcgttg cggacgtgcg tagctgaatc accctccaca aaaatgttgt tgaggtcaac
      961 cggagtgtgc cccgccgcga aaaccacttc cacggggaag gaggtggtga atcctatccg
     1021 cttattgttc atagctttgc cgcagatact gctcttccca ttcgtctatc tggctttcca
     1081 aggtcccgat ctcgctttcg agctcagcca tccgcgtttg cagggtgctg agacgtcccg
     1141 gttccgcata agtttccgag cgcgaaagct ctatgtggat ggattcaagc tcgttctgca
     1201 gttctccgag gcgttgcccg ccctgttcaa tactcagatg tatctgctcc agataccagg
     1261 gatttacctt ctttttgcgc tcacggggtg ggggaggggc cttggtcagt tccggttcct
     1321 cgaaagacag ctcgatcgct ctctgccagt cgcagtcggg ttcctccacg gtggtgtaga
     1381 ttccttctgg tgaagttttc ttccggaaaa cccagaactt ggtggccagt tggctgagga
     1441 accagcggtc gtgggaaacg aagatgattg tgccggaaaa ggctttcagc gcttccagca
     1501 gggcgtcggt catgtcaata tccagatggt tggtgggttc gtcaagtatc atcagattgg
     1561 ggttttcgtg gatcatctgg cagaggtaaa gcctggaacg ctcgccgcca ctaagcacgt
     1621 gaaccttttt atccacattg tcgccgcgga agccgaagcg cgccagccag cccagaacat
     1681 agccctgcgg tgcctgaggc acaagctgcc agatggtttc catcacggtg aggctgtcat
     1741 ccagcaatac ctgatgctgg tcgtaatagc caatatcgag gctggcaccg gttttgaggc
     1801 tgccgcccat gatctcgtgt tccccgagaa ggatgttcaa aagggtggtt ttaccgcatc
     1861 cgtttggtcc tataacacag atacgatcac ggtaatgggc tttgaggtca agctcccggg
     1921 caagctcgag ctccggcggg atgccgaaac agaggttggt gagggtgtaa acgtcattgc
     1981 cgctgcgttt tccggccgcc acgggcagtc gcagttgctt ctgctggccc ggctttttca
     2041 ctatttccat ccgctcaagc atcttttggc gcgagcgggc tgaactggtg cgggttccgg
     2101 cgatgaattt ctggatgtag gcttggattt ccgcgatgaa cttttgctgg cgctcatact
     2161 ggcgttcctg gctgaggcgg gcgatttcgt cagcggcctt gaaagagctg taattgccct
     2221 tggtaacgga tatctggcct tcgcgcaggc accagatgct gctcacggtg ttatccagga
     2281 actggcggtc gtgtgatacg atcagaaagg gtttggcttg cttcagcaga tatctttcca
     2341 accaggccat catcgccagg tcgaggtggt tggtgggctc gtccaggata agcaactcgt
     2401 gctccagcaa caggatggcg gccaggcaga tacgggtttg ctcgccgccg ctgaaatctc
     2461 cgcagcactt attccaagta tccggcggca ggttaagcga ggtgagcaca tatttaagct
     2521 cgttttcaaa ctcgtagccg cccaggctgg tgtagcgctc cacgcaaaga ttcagccggg
     2581 cttcggtctc cgcgctgtgg gtggcagcaa gttcccggga gaggcggtct atctcatctc
     2641 ccagggaaat caggtcggct cgggaggcgc gaacatgctc cagcagtgtg atgtccggtt
     2701 cgagcttgaa atcctgcggc agccaggcca cgcggcagtt cctggcgcgc aaaacctctc
     2761 cggaacttgg ctgcagtaag ccaagaaaca agcggatgag ggtcgtctta ccggaaccgt
     2821 tggccccgat cagccccacc cggctgttct gctccaaact gcagtccacc tggtccagga
     2881 tgtaattgcc gccaaactcc agcgacaaat cacgcgcacg gatcaaactc actgtatctt
     2941 cgtcttcctt tctgaaactt ggcgcttgcg gcttcaatgc acacttgcgc tagcgaatag
     3001 accatccttt ccaaaggggc ggggatgtca aatacaaatt ttgcttgtca aaaacggggt
     3061 atgttttttt taggttccac ggtccgggat gtagcgcagt ccggctagcg tacttgaatg
     3121 gggttcaagt ggtcgcag
//
LOCUS       DAOOWR010000199         3107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31420_length_3107_cov_2.936763, whole genome shotgun sequence.
ACCESSION   DAOOWR010000199 DAOOWR010000000
VERSION     DAOOWR010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31420_length_3107_cov_2.936763"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..370)
                     /locus_tag="PLB85_05385"
     CDS             complement(41..370)
                     /locus_tag="PLB85_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72799.1"
                     /translation="MEFRKESNIKKIRELLDLSENHKIELRHYKNPVLVWAVADGTAY
                     YTIWEEELLERYGLANVDGWDFEEDLLFCPDWMEDEDGFDDDLDDDDDDDADLICNFK
                     MPPPSKK"
     gene            complement(380..1513)
                     /locus_tag="PLB85_05390"
     CDS             complement(380..1513)
                     /locus_tag="PLB85_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72800.1"
                     /translation="MTEPRKHEHPEPSSDPGIEPSANKDRQPDHNTRQPVKPPVKPPR
                     NIVPIIMGLAILLLLMPRPLSAQLDIGVSMSTTYSDNVFQLSEYDITRWDHSHPNLEY
                     ANTTDDLNLGVRFDLAYPLRYQWWKFIPSVTATVSQNIFNPNKQRQDALFRLRVERYY
                     WNFTALYGYYPENYVRSYVDTDGTQHLEPYSYARNLYRGDLNLKPFKKTTLQFHGRYE
                     QYYYNQFFTEFDANATTLGIGARYAFPTFSLGANYRYRIYDNYRHDAEDGSYKSNRYS
                     GNIRLKSTPLNDEKPASPTFYPELTLSYEERFFQSQDPWYGGRADFIYNTSAALNFDF
                     GPSWNIKLDYSHAYRNVESPVEEVCRAREYSENKVSATVKYSF"
     gene            complement(1522..2337)
                     /locus_tag="PLB85_05395"
     CDS             complement(1522..2337)
                     /locus_tag="PLB85_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72801.1"
                     /translation="MFKRLILAFFCLAALSALAAKVKPLSFTDPGNRRLLKTDAGNHW
                     YYRSLPERSMTLGVEGISAIQLRSFGLDNLRKPQVTTIIGKEKKTWDLSLEQRLNGYY
                     IYRELSIPIPAGTKSIELLCYERGIYFRAFHTVKTAPKPKPAKPANLAIKAHGGVITV
                     SHNGTDSPYYVFNSSQGMKFTLNNGRSANLYVRARLLDRSLPAFQIYRNGVLLDTKEF
                     TLRRSTKYKAMGVEHLTIGMKIELAGQSGPAEYELRAVSDHMFFARPVLRKAN"
     gene            complement(2351..>3107)
                     /gene="pgsW"
                     /locus_tag="PLB85_05400"
     CDS             complement(2351..>3107)
                     /gene="pgsW"
                     /locus_tag="PLB85_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOU72802.1"
                     /translation="AVAITGSNPGVNLALYAALKTMKLEPAIIVALSSASYGANREEY
                     TWLDMEAILKKKDLIDFSSQYASLGGKDDLGIGLSDPGIKALFDAMSRTGTAQLIGAT
                     LADNVKARESAYNQLLPEGQRYRLFVNIGRGLANVGSIPNANQIREGLNPKLAEEQFD
                     PEGVMMVMAREGVPVFSMQHPRRWIRRYKLEDASEHMPEPGVGPAFSQKKHNVTVASI
                     CLALLVAAIVAVIVLDRHDRNFMANIVDPDEEL"
BASE COUNT          645 a          712 c          963 g          787 t
ORIGIN      
        1 gcttcaccaa taaccgcgac ccgcatagaa tcttccgggg ttatttctta ctcgggggtg
       61 gcatcttgaa attgcagatc agatccgcgt catcgtcgtc atcgtcatcc aggtcatcat
      121 cgaagccgtc ttcatcctcc atccagtccg gacaaaagag caggtcttcc tcgaagtccc
      181 agccgtccac gttggcgagg ccgtatcttt ccagcagttc ctcttcccag atggtgtaat
      241 aggctgtgcc gtccgccacc gcccagacca gaacgggatt tttgtaatgg cggagctcga
      301 tcttgtggtt ctcggaaaga tcgaggagtt cgcggatctt tttgatgttg ctttcttttc
      361 tgaattccat tgttctcacc taaaagctgt atttaactgt ggcagaaacc ttgttctcgc
      421 tgtattccct ggcgcggcag acttcttcca caggtgattc tacattcctg taggcgtgag
      481 agtagtcaag ctttatattc caactagggc caaaatcgaa attgagggcg gcagaggtgt
      541 tgtagataaa atcggcgcgg cctccatacc agggatcctg ggattggaaa aagcgttcct
      601 cgtaggatag ggtgagttca ggataaaagg tggggctggc tggtttctca tcgtttaggg
      661 gagtgctttt gaggcggatg ttgccactgt agcggttgct tttataactg ccgtcttctg
      721 cgtcatggcg gtagttgtcg taaatccggt agcggtagtt ggcgcccagg ctgaaagtgg
      781 gaaaggcgta acgggcaccg atgccgagag tggtggcgtt ggcgtcgaat tcagtgaaga
      841 actggttgta ataatattgc tcgtagcgac cgtggaactg aagcgtggtt tttttaaagg
      901 gtttgagatt aaggtctccg cggtagaggt tgcgggcata gctgtatggt tcaaggtgtt
      961 gggtgccgtc ggtatccacg tagctgcgca cgtagttttc aggataatag ccgtagaggg
     1021 cggtgaaatt ccagtaatac cgttccacgc ggaggcggaa aagtgcgtcc tggcgttgtt
     1081 tgtttgggtt gaatatgttt tgggaaacgg tggcggtaac ggagggaatg aatttccacc
     1141 attggtagcg gagagggtaa gccagatcga aacggacacc gagattgagg tcgtcggttg
     1201 tgttagcgta ttcgaggttg ggatggctat ggtcccagcg ggtgatgtca tattcggaga
     1261 gttggaacac attatcggag tatgtggtgc tcatagagac gccgatgtcg agttgcgcgg
     1321 agagcggccg aggcatcagg agcagtaaga tagccaagcc catgatgatg ggaacgatgt
     1381 tgcggggtgg tttcaccggc ggtttaactg gttggcgtgt attatgatcc ggctgccggt
     1441 ccttgttagc tgagggttct attccaggat cggaggaggg ttcgggatgt tcatgtttgc
     1501 gtggttcggt catgctgttc ctcaattggc cttgcgcagc accggccggg cgaaaaacat
     1561 gtggtcgctc acggcgcgca gttcatattc agcaggcccg ctttgccccg caagttcgat
     1621 tttcatgcct atcgtgaggt gttccacacc catagctttg tatttggtgc tccggcgcaa
     1681 agtgaattcc ttggtatcca gaagaacacc gttgcggtag atttgaaaag ccggcaggct
     1741 gcggtccagc aatctggctc gcacatagag gttggcagag cggccgttgt tgagggtgaa
     1801 tttcatccct tgggaagagt tgaaaacgta ataagggctg tccgtgccgt tgtggctgac
     1861 cgtgatcacg ccgccgtggg ccttgatggc aaggttggct ggttttgcgg gcttgggctt
     1921 gggtgcggtt ttaacggtat ggaaagctcg gaaatagata cctcgctcat aacaaaggag
     1981 ttcgatgctt ttagttccgg cggggatggg gatgctgagt tcgcggtaga tgtaatagcc
     2041 gttgagacgc tgctccaggc ttaggtccca agtttttttc tccttaccga tgattgtggt
     2101 gacctgaggt ttgcgcaggt tgtccagacc gaaggaacgc agctgaatgg cggaaattcc
     2161 ctcaactccc aaggtcattg atctttccgg cagggaacgg tagtaccagt ggtttccggc
     2221 atccgtttta agcaaacggc ggttgccggg gtctgtgaaa ctgaggggct tgaccttcgc
     2281 tgccagagcg gaaagtgccg cgagacaaaa aaaggcaagg atcaggcgtt tgaacattta
     2341 tgctccaaaa tcataattct tcatctgggt ccacgatgtt ggccatgaaa ttgcggtcat
     2401 gccggtccag gacaataacc gccacaatgg cagccacaag cagggcaagg cagatggaag
     2461 ccacggtgac attgtgtttt ttctggctga aagcggggcc gacacctggc tccggcatat
     2521 gttctgaggc gtcttccagc ttgtagcggc gaatccagcg ccggggatgc tgcatgctga
     2581 agaccggaac gccttcgcgg gccatgacca tcatcacgcc ctcgggatcg aattgctctt
     2641 cagccagctt ggggttaagg ccttcccgga tctgattggc gtttgggatg ctgcccacgt
     2701 tggccaggcc gcggccgatg ttcacgaaca ggcggtagcg ttgtccctcc ggcagcaact
     2761 ggttgtaagc gctttcgcgg gctttgacgt tgtctgccag ggtggcgccg atgagctggg
     2821 cagtcccggt tcgggacatg gcatcgaaca aagctttgat accagggtct gaaagcccta
     2881 tgccaaggtc atccttgccg ccaagtgagg cgtactggct gctgaaatca atcaggtctt
     2941 tctttttgag aatggcttcc atgtcaagcc aagtgtactc ttcccgattg gcgccgtaag
     3001 aagcggaaga gagggccacg atgatggcgg gttccagctt catcgttttc agcgcagcat
     3061 aaagggccag gttcacgccg gggttcgagc cggtgatggc aacagct
//
LOCUS       DAOOWR010000200         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31456_length_3104_cov_3.661856, whole genome shotgun sequence.
ACCESSION   DAOOWR010000200 DAOOWR010000000
VERSION     DAOOWR010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31456_length_3104_cov_3.661856"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLB85_05405"
     CDS             <1..321
                     /locus_tag="PLB85_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72803.1"
                     /translation="IVEYGSAADDDVIPAYTLDAKNYPNPFNPETTIAFSVPKTGPAS
                     LKVYNTKGQLVRTLVNDVREAGNHSVVWNGMDEQGNSVSSGLYFYRLSSDGNTVTRKM
                     LLAK"
     gene            482..2809
                     /locus_tag="PLB85_05410"
     CDS             482..2809
                     /locus_tag="PLB85_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU72804.1"
                     /translation="MRKHILPLLLMLVAGRLFSATVSGFVTREDSGEPLQYVSVRVAG
                     TKIGAQTNKQGYYVLTVNQTGSFKLEFSLVSHQRESKSFSIRSQSDEIVLNAQIKKSA
                     VKLNKIVVTADAESGLEGPVIRTSSIHRGREDIQAMVSVVEADVFRAVLTLPGVMPIS
                     DFFSGLYVRGGSPDQNLTLLDDTDVYNPSHLGGLFSTFNTDAVENVELIKGGYPAKFG
                     GRLSSVLDVTNRQGNRVKSQGVARVSLISSSATLEGPWSVGSADGSYMASFRRTYLEL
                     IKTIYDKLPDYYFYDGHAKFNLDADNSNKLSFSGYIGRDDLSYDLGSVLDVDWGNSNI
                     SARWLHLFSPRLYSNFTLAGSQFSSGMSQTAAEGDETIFRTDNSIKDLTAKGILNWKP
                     NNSHEVESGFEVKWNDTALKMETSYQIDPDGLPDGAISSFTSSLFLQDTWVINPLWTL
                     QPGLRLNWYRTGRIIPDQIPAANYLNLEPRLSFRWTLDAGESVYTNFGVYNQYLTLLA
                     MDVNTPFDVWLPLDGSLRPARSLHYILGYQRRLGKHLALETEAYYKDYDNLAQLDFNA
                     FFNWNNSTGNLGSAMRVGIGHAYGAELLLRNDWNGLEGFIGYTFSRTRRKIEGLNLDP
                     QTGEASYFFPRFDRSHSVSMVESYNLSANTGWQPLGADFKLGLNFSYNSGQPVEVPER
                     IYFDGDSFQIIYSYKDGHRLPYYIRLDLSAKLQWNTRWGSVEPYLDVINVFGRKNVSF
                     RTYQLVPQEDLTLRLETQDGSQFPFLPFIGVNVTW"
     gene            2803..>3104
                     /locus_tag="PLB85_05415"
     CDS             2803..>3104
                     /locus_tag="PLB85_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72805.1"
                     /translation="MVGKHLFRLMPLLLLALLLASCENTSGPRFEGDVFTVAGLLKAG
                     QPVNFEYPIYVTRSSAIEDFNPLEIFVYDATVIIRDLDADLEFQLTPALHEFKFKY"
BASE COUNT          712 a          968 c          790 g          634 t
ORIGIN      
        1 atagtggaat acggcagcgc cgccgatgac gacgtgatcc cggcttacac cctggacgcg
       61 aagaactatc ccaacccctt caatcctgaa accaccatcg ccttcagcgt tcccaaaacc
      121 ggccctgcca gcctcaaggt ttataacact aaaggccagc ttgtccgcac cttggtgaac
      181 gatgtccgcg aagccggcaa ccacagcgtt gtctggaacg gaatggacga acagggcaac
      241 agcgtttcca gcggactgta tttctatcgc ctcagcagcg acggcaatac cgtcacccgc
      301 aagatgctac ttgccaaata atccacaaca atctggctga aaagtcacaa catactggga
      361 gcgacccctc gtggccgctc cctttttgct tgagcggcgg gagctgggaa atttgtggtt
      421 gacagctttg aagccccgaa actctttaat cgcactcgaa aaccaaatca tgggagaatt
      481 gatgcgtaaa cacatactgc cgctactgct gatgcttgtg gccgggaggc ttttcagcgc
      541 caccgtgagc ggatttgtga cccgcgagga cagcggcgag cccctgcagt atgtgagcgt
      601 gagggtggct ggcaccaaaa tcggcgcgca gacgaacaaa cagggatatt acgtgctcac
      661 ggtcaaccag accggcagct tcaagctgga gttttccctg gtctcccacc agcgggaaag
      721 caaaagcttc agcatccgct cccagagtga cgaaatcgtc ctgaacgccc agataaaaaa
      781 gtccgcggtg aaactcaaca aaatagtggt gacagccgac gccgagtccg gcctcgaagg
      841 ccccgtgatc cgcaccagtt ccatacaccg gggccgggag gacatccagg caatggtttc
      901 cgtcgtggag gcagacgtgt ttcgcgcggt gctcactctc cccggcgtga tgcccatttc
      961 tgacttcttt tcaggacttt acgtgcgggg cggttcgccc gaccagaatc ttaccctgct
     1021 ggacgacacc gatgtttaca accccagcca tctgggtggg ttgttcagca ctttcaatac
     1081 cgatgcagtg gaaaacgtag aactcatcaa gggcggctac cctgccaagt tcggcggcag
     1141 gctgtcttcc gtgctggatg tcaccaaccg ccagggcaac cgggtcaaat cccaaggcgt
     1201 ggctcgggta agcctcattt catcttccgc cacgctggaa gggccttgga gtgtcggttc
     1261 cgcagacggg tcctacatgg cttcgttccg ccgcacctat ctggaattga taaaaaccat
     1321 ctacgacaaa cttccagact actatttcta cgacggccat gccaagttca acttggacgc
     1381 ggacaacagc aacaagctca gtttcagcgg ctacatcgga cgcgacgacc tttcttacga
     1441 tttgggaagc gttctggacg ttgactgggg caacagcaat atctccgcgc gctggctgca
     1501 tctcttttcc ccgcgcctct actccaattt cacgctcgcc ggaagtcagt tcagcagcgg
     1561 aatgagccag accgccgcag aaggggacga aaccatcttc cgcaccgaca attccatcaa
     1621 agacctcacc gccaagggga tactgaactg gaagccgaac aacagtcacg aggtggaaag
     1681 cggtttcgaa gtgaagtgga atgacaccgc cctgaagatg gaaacctcct atcaaatcga
     1741 tccggacggg cttccggacg gcgccatctc ttcgttcaca tcctcccttt tcctgcagga
     1801 tacatgggtg ataaaccctc tctggacctt gcagccgggc ctgcggctga attggtatcg
     1861 cacgggaagg atcataccgg accagattcc cgccgcgaac tatctgaacc tggaaccccg
     1921 cctttccttc agatggaccc tcgacgccgg agaaagcgtc tataccaatt ttggcgttta
     1981 caaccagtat ctaaccctgc tggcgatgga cgtgaacacc cccttcgacg tctggctgcc
     2041 gctggacggc tcgctgcgcc cggcccgctc gctgcattac atcctcggct accagaggcg
     2101 cctgggcaag catctggccc tggaaaccga agcctattac aaggattacg acaacctagc
     2161 ccaactggat tttaacgcct ttttcaactg gaacaacagc accggcaacc tcggcagcgc
     2221 catgcgtgtt ggcattggcc acgcctatgg ggcggaattg ctgctccgca acgactggaa
     2281 cggcctcgaa ggctttatcg gctacacctt cagccgcacc cgacgcaaga tagaaggcct
     2341 caaccttgat ccccaaaccg gcgaggccag ttatttcttt ccccgatttg accgcagcca
     2401 cagcgtctcc atggtggaaa gctacaacct cagcgccaac accggctggc agcccctcgg
     2461 cgcggatttc aagcttggcc tgaacttcag ctacaattcc ggccagcccg tggaggtgcc
     2521 ggagcgtatc tatttcgacg gcgacagttt ccaaatcatc tacagctaca aagatggcca
     2581 ccgcctgccc tattacatac ggctagacct gagcgcaaaa ctgcagtgga acacccgttg
     2641 gggcagcgtc gaaccctatc tagacgtcat caatgtcttc ggccgcaaaa acgtaagctt
     2701 ccgcacttac caactcgtgc cgcaggaaga cctgaccctg cggctggaaa ctcaggacgg
     2761 ctcccagttc cccttcctgc ctttcatcgg agtgaacgtc acatggtagg caagcatctt
     2821 ttccgcctta tgcccctgct gcttctggcg ctgctgcttg cctcctgcga aaacaccagc
     2881 gggccccgct ttgagggcga cgttttcacc gtggcgggcc tgctgaaggc cggtcagcct
     2941 gtgaactttg agtatcccat atatgtgacc cgcagttccg ccatcgagga tttcaacccc
     3001 ctggaaatct tcgtttacga cgccaccgta atcatccgcg acttggatgc cgacctggag
     3061 ttccagctca cccccgctct gcacgaattc aaattcaaat acat
//
LOCUS       DAOOWR010000201         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31592_length_3094_cov_3.057256, whole genome shotgun sequence.
ACCESSION   DAOOWR010000201 DAOOWR010000000
VERSION     DAOOWR010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31592_length_3094_cov_3.057256"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PLB85_05420"
     CDS             <1..671
                     /locus_tag="PLB85_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOU72806.1"
                     /translation="DVYDGYKRNYDEMMAPGMVLDAGAPSFEEKAFHDFHMYTLDQKV
                     SFANNQTKQLELYPLQNIKASQSYEYSTWDSGVNSVIKFKNTEENGIGKPLPKGTVKV
                     YKQDSDGNLEFIGEDSISHTSKNEEVKINTGTAFDLVASTLVRDHKDLGSRRSERTIQ
                     VTLRNNSAEAKTINVLHQLGGNDRITESNFSHTEDTNMKSTFEVPIQSDKEVILSFTV
                     RTEY"
     gene            696..1136
                     /locus_tag="PLB85_05425"
     CDS             696..1136
                     /locus_tag="PLB85_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU72807.1"
                     /translation="MPDKIYSNLNGIRREMLKILGEVSSLTKSPLAIEDAIDDFWHPK
                     CDVYQTETQWVVLVELAGVNKDEINISASSEYLRVSGTRVLHSEDCQTTYYSMEIDTG
                     RFDRRIFFPDLNLDKEHPKVQYVNGILRIAFTIQAMVERIIPIE"
     gene            1233..2171
                     /locus_tag="PLB85_05430"
     CDS             1233..2171
                     /locus_tag="PLB85_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72808.1"
                     /translation="MKRTLILALALGAWLLCAAAEVESVRSTDTSKTSNTVCIELSEP
                     AQYTTQNLPGGKGLRLTIKNVSSLGGKPQYPRLSEVIDVVSARIEGRDAIIDIKTMGD
                     FDISHSSNSDRDRITVAVNSAPKTNIPPSAMPKKVALPENVSNRPKQASPAPSVKAPG
                     SKSPQEQPSAQTAPPSQMPLPASEEEQLEAKALPETTTAGTSGLEANAVNDDKTTKGN
                     LPLWLIIAAAALLILLVVLKFFLAKRPEAVSGTSETEGDENPAEGRTLLLDPETRQRM
                     VQKLLDQGWSSSEIAREMRLGVREVEEIVAKLKGLK"
     gene            2168..>3094
                     /locus_tag="PLB85_05435"
     CDS             2168..>3094
                     /locus_tag="PLB85_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72809.1"
                     /translation="MMGRADWRAVFNRLALLLLLALAVGTGHAQTEFTNTAFYPDVRS
                     QLLPSPLAENETYILDDPLILGSGVHAPFESWKAFKSSLLELFLPGSPQRALLQYAYR
                     DSSLSYRLETNLFTGWDQRWDPEGVYGLHSLGLRVNSTFNDRFRARGIYWNGKFYGDR
                     SAARHSPLIDGSCSASTNNLFVDNINGELSYGGEHLSAALGRGRFQIGNSLSGSIVLS
                     DQVNEYDYALLEQRVGQFSFSWLHASLVADTLVAGLHPAKYTDIQQLTWQPRPFLDMF
                     YGGTVTYGNRIDLSYLLPTVFIRASGFSNAKVD"
BASE COUNT          785 a          885 c          820 g          604 t
ORIGIN      
        1 ttgacgtgta tgacggctac aaacgcaatt acgatgaaat gatggcgccg ggcatggtcc
       61 tggatgccgg agcgcccagc ttcgaggaaa aggccttcca cgacttccat atgtacaccc
      121 tggaccagaa ggtgtccttc gcgaacaacc agacaaaaca gttggaactc tatcccctgc
      181 agaacatcaa ggccagccag tcttatgaat acagcacctg ggacagcggc gtgaacagcg
      241 tgatcaagtt caaaaacacc gaggaaaacg gcatcggcaa gcccctgccc aaaggcaccg
      301 ttaaggttta taagcaagac agcgacggca acctggaatt catcggcgag gacagcatct
      361 cgcacaccag caagaacgaa gaggtgaaga tcaacaccgg caccgccttc gacctggtgg
      421 cctccaccct ggtgcgggac cataaggacc tgggctcccg cagaagcgaa cgcaccatcc
      481 aggtgaccct gcgcaacaac tccgccgagg cgaaaacaat caatgtgctg caccaactgg
      541 gcggtaacga caggatcacc gaaagcaatt tcagccatac cgaggacacc aacatgaaat
      601 ccaccttcga ggttcccatc cagtcggaca aagaggtgat cctcagcttc accgtgagaa
      661 ccgaatacta atgtaactaa cattggggat ggaagatgcc tgacaaaata tattcgaacc
      721 taaacggcat ccgccgtgaa atgctgaaaa tcctgggcga agtttcctcg ctcacaaaaa
      781 gccccctggc catcgaggac gccattgacg atttctggca tcccaaatgc gacgtttacc
      841 agaccgaaac gcagtgggtg gtgctggtgg aactggccgg ggtgaacaaa gacgagataa
      901 acatctcagc cagcagtgag tatctgcgcg tcagcggaac ccgcgtactg cattcagagg
      961 actgccagac cacatattac agcatggaaa ttgacactgg cagattcgac cgccggatct
     1021 ttttccccga cctcaacctg gataaagaac atcccaaggt gcagtacgtg aacggtatcc
     1081 tgcggatcgc cttcaccatc caggccatgg tggaaagaat cataccaata gaatgaagca
     1141 cagactccat ccctccggtt ttggctggat ggctggagat tttatactga tttactgagc
     1201 ggacggccaa gcgctgtctg gatggaggaa aggtgaaaag aacattgatt ctggcgctcg
     1261 ctttgggcgc ctggctgctc tgcgcggccg cggaggtgga atcggtacgc tcaaccgata
     1321 cttccaaaac ctcaaacacc gtgtgcatcg aactaagtga accagcccaa tacacgacgc
     1381 agaacttgcc cggaggcaaa ggcttgcgcc tcaccatcaa gaatgtaagc tctctgggcg
     1441 gcaaacccca gtatccgcgg ctgagtgaag tcatcgatgt agtttcggcc cgcattgaag
     1501 gcagagacgc catcatcgac atcaagacca tgggcgattt tgacataagc catagcagca
     1561 attcagaccg ggaccgcatc acggtcgctg tgaactccgc gcccaaaaca aacatccctc
     1621 cctcggccat gcccaaaaag gtggccctgc cggaaaacgt cagcaaccgt cccaaacagg
     1681 catcccctgc gccttcggtg aaagctccgg gttccaaatc cccacaggaa cagccttccg
     1741 cgcaaaccgc cccgccgtcc cagatgccgt tgcccgcgag cgaagaagaa cagctcgaag
     1801 ccaaagccct gccggaaaca accacagccg gaacttccgg gcttgaggcg aatgctgtga
     1861 acgatgacaa aacaaccaag ggcaatctgc cgctttggct gatcattgcg gctgcggcac
     1921 tgctgatcct gctggtggtc ttgaagttct ttctggccaa aaggccggaa gccgtttccg
     1981 ggacttccga aacagaaggg gacgaaaatc ccgcagaggg caggactttg cttctggacc
     2041 ccgaaaccag gcagcgcatg gtgcagaagc tgctggacca gggctggtct tccagcgaaa
     2101 tagcccgcga gatgcgtttg ggagttcggg aagtggaaga aatcgtggca aaactcaaag
     2161 gactgaaatg atgggacgcg cggattggag agccgttttc aaccgcctgg ctttgctgct
     2221 gttgctggcg ctggcagtcg ggacgggcca cgctcaaaca gaattcacga ataccgcctt
     2281 ctacccagat gtgcgcagcc agcttttgcc cagccccctg gccgaaaatg aaacctatat
     2341 actggatgat ccgctcatcc tgggttcagg cgttcacgcg ccttttgaaa gctggaaggc
     2401 gttcaagtcc tccctgctgg agcttttcct gccgggttca ccccaacggg ctttgctcca
     2461 gtatgcatac agggattcca gtttgagcta ccgccttgaa accaatttat tcacaggctg
     2521 ggaccagcgt tgggaccccg agggcgttta cggcctccat tcactgggct tgagggtcaa
     2581 ttccactttc aacgacagat tcagggccag aggtatctat tggaacggca agttttacgg
     2641 cgatagatcc gctgccaggc attccccttt gatcgatgga tcatgttccg ctagcaccaa
     2701 caatcttttc gtcgataata tcaatggcga gctcagctat ggcggagagc acctgagcgc
     2761 cgcgctgggc cggggacgtt tccagatcgg caattcactt tccggttcca ttgttctttc
     2821 agaccaggtg aatgaatacg attatgcgct actggaacaa agggttggcc agttcagttt
     2881 ttcctggctg catgcttccc tggtggcgga taccctggtg gctggcctgc atcctgccaa
     2941 atataccgac atccaacagc tcacctggca gccaaggccc tttctggata tgttttacgg
     3001 cggaacggtg acctacggca accggatcga cctcagctac ttgttgccga ccgtattcat
     3061 ccgcgcgagc ggattctcga acgccaaagt ggac
//
LOCUS       DAOOWR010000202         3074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31834_length_3074_cov_3.613779, whole genome shotgun sequence.
ACCESSION   DAOOWR010000202 DAOOWR010000000
VERSION     DAOOWR010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31834_length_3074_cov_3.613779"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /locus_tag="PLB85_05440"
     CDS             complement(<1..1892)
                     /locus_tag="PLB85_05440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU72810.1"
                     /translation="MIEVTLNGTQVQTEAGITILELARRNGIEIPNLCHDEELKPFGS
                     CWVCAVEVKGRRGFVTSCGTKVLPGMEIITDSEDIRAARKMALELLISDHYADCEAPC
                     KIACPDHVDIQSYVSLIANGQYHDAVKVIKETLPMPLSIGRVCPAFCEKECRRQIIEE
                     PIAIRQLKRFAADEDLGDVWNYVPEKAEATGKKIAIIGAGPSGLTCGFYLSNLGHEVT
                     VFESAPAAGGWLRYGIPEYRLPKDILDREIELMCANGMKIEYNVQLGRDLQLEKLSID
                     YDAVYLAIGAQKAVPMPVKGSELAGCYLGVDFLKAHSLGNTPVLGKKVAIVGGGNTAI
                     DCARTAIRLGCEVSVIYRRTKAEMPAEPFEIEAAGHEGVVFHYLCNPVEYFGENGALR
                     EVKIERMRLGEPDASGRRRPEPTGEFFTESYDSIIAAISQVPEVDIFAEEANRVEGKE
                     LPISRWQTVVVDESTMHTGLANVFAGGDFRRGAATAIEAIADGRLAAEAIHRYLAGEE
                     IKVDHFRFDAKKGHKVQDISQEEFAQFEMIARKVMPEIPLDEAKSTFNEVETGFDADE
                     AKAEAARCLECGCQVNETCKLREYCTDFRVDALHFPGSINKHPIDYSHPYIVRDANKC
                     INCGRCV"
     gene            complement(1902..>3074)
                     /locus_tag="PLB85_05445"
     CDS             complement(1902..>3074)
                     /locus_tag="PLB85_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU72811.1"
                     /translation="HSVVEGMMIAAAAVGADEGYVYVRMEYPLACTRIRKAISVAEEL
                     GILGDNVFGSGKSFRISVVEGAGAFVCGEETALLASIEGKRGMPNPKPPFPAESGLYG
                     KPTVINNVETLALVPVVMREGVEAFRKVGVAKSPGTKTFALTGHVANTGLIEVPFGTT
                     LRQIIFEIGGGVLDKDGKINNEAFKAVQIGGPSGGCLTREHLDMSLDYDSLGAVGAMV
                     GSGGLVVMNDSSCMVKIAHFFMSFIQNESCGKCTFCRIGTRRMLEILTRITEGKGVLE
                     DIDRLEELSVNINKASLCGLGQTGPNSVLTTLRYFKHEYLAHIEEKRCPAGVCTALLR
                     YEIIPEKCIGCTACARKCPAQCITGTVKQPHVIDQDKCIKCSSCYKACKFNAISKG"
BASE COUNT          631 a          883 c          838 g          722 t
ORIGIN      
        1 acacagcggc cgcaattaat gcacttgttg gcgtcgcgca ctatgtaagg gtggctgtaa
       61 tcgatgggat gtttgttgat gctgccaggg aagtggagag cgtcgacccg gaaatcggtg
      121 cagtattcac gcagcttgca ggtttcattc acctggcagc cgcattccag gcagcgcgcg
      181 gcttcggctt tggcctcgtc tgcgtcaaag ccggtttcca cctcgttgaa agtgctttta
      241 gcctcatcca aaggtatctc tggcatcact ttccgcgcga tcatttcgaa ttgggcgaat
      301 tcttcctggg atatatcctg caccttgtgg cctttcttgg cgtcgaagcg gaagtgatcc
      361 actttgattt cctcgcccgc cagatagcgg tggatggctt ccgcagccag acgcccgtcg
      421 gctatggcct cgatggctgt agcagccccg cggcggaaat cgcccccggc aaaaacgttg
      481 gccaaacccg tgtgcatggt gctttcgtcc accacgaccg tttgccagcg gctgatggga
      541 agctcttttc cttccacgcg gttggcttcc tcagcgaaga tatcaacctc cggcacttgg
      601 gagatggcgg caatgatgga gtcgtagctt tcggtgaaaa actcgccggt gggttcggga
      661 cgccttcttc cgctggcatc gggttctccg agcctcatcc tttcgatctt cacttcacgc
      721 agggcaccgt tttcaccgaa atactcaacc gggttgcaga ggtagtggaa aaccacaccc
      781 tcgtgtccgg cagcctctat ttcaaagggt tcggcgggca tttcagcctt ggtgcggcga
      841 tagataacgc tcacctcgca accgaggcga atggcggtgc gggcacagtc tatggcggtg
      901 tttccgccac caacgatggc cacttttttg cccagaaccg gggtgttccc caagctgtgg
      961 gctttgagga aatccacgcc cagataacag ccggcgagtt ctgagccctt aaccggcatg
     1021 ggcactgctt tttgggcccc gatggccaga tagacggcgt cgtaatcaat actcagcttt
     1081 tccagctgta gatcacgtcc caattgaacg ttgtattcga tcttcatgcc gttggcacac
     1141 atcagctcga tctcacggtc cagtatgtct ttgggcagcc tgtactcagg aatcccatag
     1201 cgcagccagc ctccagccgc gggggctgat tcaaagaccg tgacctcatg gccgaggttg
     1261 gaaagataga atccgcaggt gagccctgaa ggcccggctc cgataatggc gatcttttta
     1321 cccgtggctt cagccttctc gggaacgtag ttccagacgt cgccgagatc ctcatccgcg
     1381 gcaaagcgtt tcagctggcg gatggcgatg ggttcctcaa tgatctggcg gcggcattcc
     1441 ttttcgcaga aagccgggca gacccgtcct atggaaaggg gcatgggcag ggtttctttg
     1501 atcaccttca cggcatcgtg atactggccg ttggcgatca gcgaaacgta actctggatg
     1561 tccacatggt ccgggcaggc gatcttgcag ggggcttcgc aatcggcata atggtccgag
     1621 atcagcagtt ccagcgccat cttgcgggcg gcgcggatgt cctcgctgtc cgtgatgatc
     1681 tccattccgg gaagcacttt ggtgccgcag gaagtcacaa atccgcggcg gcccttcact
     1741 tcaactgcgc agacccagca ggagccgaat ggtttcagtt cttcatcgtg gcaaaggttg
     1801 ggtatctcga tcccgttgcg gcgagccagt tccaggatgg tgatcccggc ttcggtctgc
     1861 acttgggttc cgttcagcgt tacttcaatc atctgattca cctagccttt gctgatggca
     1921 ttaaacttgc aggctttgta gcaggagctg cattttatgc atttgtcctg atctatcaca
     1981 tgcggttgtt tcactgtccc ggttatgcat tgcgcagggc acttgcgcgc gcaagctgtg
     2041 cagccgatgc acttttccgg tattatctca tagcgcagca aggctgtgca aacaccggcg
     2101 gggcagcgct tttcctcgat gtgcgccaga tattcgtgtt tgaagtagcg gagggttgtg
     2161 agcaccgaat ttggccccgt ctggccgagg ccgcagagcg aagctttgtt gatgttcacg
     2221 gaaagttcct ccagacggtc gatatcttcc aaaacccctt tgccttccgt gatgcgggta
     2281 agtatttcca gcatgcgcct ggtgccaatg cggcagaaag tgcatttccc acaggattca
     2341 ttttggatga agctcatgaa gaaatgagcg atcttaacca tgcaactgga gtcattcatc
     2401 acaaccagac caccggaacc aaccatggcc ccaactgccc ccagagaatc ataatccaga
     2461 gacatatcaa gatgttcgcg tgtgaggcaa ccaccggaag gaccgccgat ctgaaccgcc
     2521 ttgaaggctt cgttattgat cttgccatcc ttatccagca ctcctccacc gatctcaaag
     2581 atgatctgac gcagagtggt gccaaagggg acttctatca aacctgtatt ggcaacatgt
     2641 cccgtgagcg caaaagtctt ggttcccgga gatttggcaa cgccaacttt ccggaaagcc
     2701 tcaacgcctt cccgcatcac caccggcacc aaagccagag tctcaacgtt attgatcaca
     2761 gttggtttgc catagagacc gctttcagca gggaaaggcg gcttgggatt gggcatgccg
     2821 cgcttgcctt cgatggaagc cagcagagcc gtttcctccc cacagacgaa agctccggca
     2881 ccttcaacaa cgctgatgcg gaagctcttg ccgctgccaa agacgttgtc gcccagaata
     2941 ccgagttctt ccgccacgct gatagctttg cgaatgcggg tgcaggccag aggatactcc
     3001 atgcgcacat agacatagcc ctcatcggcc ccaactgccg ccgcggctat catcatgcct
     3061 tccaccacac tgtg
//
LOCUS       DAOOWR010000203         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31856_length_3073_cov_2.786282, whole genome shotgun sequence.
ACCESSION   DAOOWR010000203 DAOOWR010000000
VERSION     DAOOWR010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31856_length_3073_cov_2.786282"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..1811)
                     /gene="pruA"
                     /locus_tag="PLB85_05450"
     CDS             complement(192..1811)
                     /gene="pruA"
                     /locus_tag="PLB85_05450"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOU72812.1"
                     /translation="MFFKVPTPKNEPVKTYAPGTKERDLLKAKLDELGRQTMEIPAII
                     DGKEVFSGNTANCIEPHNHQHVIAKYHKCGEAEVNLAIQAAMKAKKDWEQMSFYHRAA
                     IFLKAAELLSTKYCALLNAATMMGQSKSAFQAEIDSTCELTDFWRFNPYFAQQIYEQQ
                     PLISPKGEWNTSEYRALEGFVFAVTPFNFTAIAGNLPTAPALMGNTVVWKPASTAVYS
                     GYFVMKILMEAGLPAGVINFVPASGAAIGNIITDSPHLAGVHFTGSTEVFNQIWLRTA
                     NNISRYKSYPRIVGETGGKDFIVAHASADVKALAVACLRGAFEYQGQKCSAASRLYIP
                     DSLYDQWFQEMSSMLATVKMGEVQDFSNFVNAVIDKSSFDKIKGYIDKARNSSDAEIV
                     WGGKCDDSRGYFIEPTIIKATNPRFCTMQEEIFGPVLTVHAYPEKNFVETLELCDTTS
                     PYALTGSIFAQDRTAINIASEKLRHSAGNFYINDKPTGAVVGQQPFGGSRVSGTNDKA
                     GSALNLQRWISARSIKENFVPATDYRYPFMD"
     regulatory      complement(1883..1998)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(2166..2241)
                     /locus_tag="PLB85_05455"
     tRNA            complement(2166..2241)
                     /locus_tag="PLB85_05455"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2206..2208),aa:Thr,seq:cgt)
     gene            complement(2241..2921)
                     /locus_tag="PLB85_05460"
     CDS             complement(2241..2921)
                     /locus_tag="PLB85_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72813.1"
                     /translation="MRRKYFMFVLLFLLAAGLMAQESTAALRFKLLEADEYELDMFYS
                     TELQRLYMQVDFELPKSSLDPDYYYGIFLHKDAQIQAITVSGSFEAHYWANNLQPEHF
                     EPHLPQPELLVWNSPARFFGIHLNSLKKYPETPHFRIWYYIEVPPFRLDESNKLTTGL
                     DAADFWYPRNISRESTVKVTMTTTPYISLRVGNSFASKLDKQYARTHKNTYVEIPAQP
                     CGFRLIRD"
BASE COUNT          707 a          699 c          837 g          830 t
ORIGIN      
        1 cgagcgctca gggtctgact ggcaggacat tgctgtggat aaatcctccg gactcgaaac
       61 aacactatgc ccactcgatt tgtcagagtg tctttgttct tttacactgg ggcggataca
      121 gcattatttg ggtttgtaaa aaaagacggg atgattggat catcccgtct ttagaggtat
      181 ttgatctgtg tttaatccat aaaggggtaa cggtagtcag tagctggaac gaagttttct
      241 ttgatcgacc ttgccgaaat ccaacgctgc aggttcaatg ccgatcctgc cttgtcgttg
      301 gtaccggaaa ccctgcttcc gccaaaaggc tgctgaccga ccacagctcc tgtgggcttg
      361 tcgtttatat agaagttgcc agcgctatgt ctcagctttt cggatgcgat gttaatagcg
      421 gttctgtcct gtgcgaaaat ggagccggtg agggcgtagg gggaggttgt atcgcaaagc
      481 tcaagggttt caacaaagtt cttttccggg taggcatgga cggttagcac cgggccgaaa
      541 atctcttcct gcatggtgca gaatcgagga ttggtggcct tgatgatggt cggctcgatg
      601 aaatagccgc ggctgtcatc acacttgcca ccccaaacga tctctgcgtc tgatgagtta
      661 cgggctttgt cgatgtatcc tttgatttta tcaaagctgc tcttatcgat cacggcattt
      721 acgaaattcg agaagtcctg aacttcgccc atcttgaccg tggccagcat ggagctcatt
      781 tcctggaacc attggtcata gaggctgtcc ggaatgtaga gcctggatgc ggcactgcat
      841 ttctgtccct gatattcgaa ggcgccgcgc aagcatgcca ctgccagggc tttgacgtct
      901 gcggaagcgt gcgcaacgat gaagtccttg ccgcccgttt caccaacgat tctgggatag
      961 gatttgtagc ggctgatgtt gttggcagtg cgcagccaga tttggttgaa cacctctgtg
     1021 cttcctgtga aatggacgcc ggcaagatgg ggggaatcgg tgattatatt tccaatagca
     1081 gctcccgaag cggggacgaa gttgataaca cccgcgggta agcctgcttc catcaatatc
     1141 ttcatgacga aatagccgct atacactgcg gtacttgccg gtttccacac aacggtgttt
     1201 cccatgaggg ccggggcggt gggaagattg ccggcaatgg cagtaaaatt gaaaggtgtg
     1261 acggcaaaga caaagccttc caaagcgcgg tattcagagg tgttccattc acctttgggg
     1321 gaaatgaggg gttgctgttc gtaaatctgt tgggcaaaat agggattgaa acgccaaaag
     1381 tcagtcagtt cacaggttga gtcaatttcg gcttgaaagg cacttttgct ttgccccatc
     1441 atggtggcgg cattcaacaa agcacagtat tttgtggaca gcaattcggc agccttgagg
     1501 aagatggccg cgcgatggta gaaagacatt tgctcccaat ctttttttgc cttcatggcc
     1561 gcttgaatgg caagattaac ttccgcttcg ccgcatttgt ggtattttgc gatgacatgc
     1621 tgatggttat gcggttcgat gcagttggcg gtgttgccgc taaacacttc tttgccgtca
     1681 atgattgctg gtatttccat agtctggcgg ccgagttcat ccaatttagc cttcaacaag
     1741 tctctttctt tggttccggg ggcataggtt ttcacgggtt cgtttttggg tgtgggtact
     1801 ttgaagaaca tttctatctc ctatgttttg ttatttcagg atgttaaaag gaaacgcact
     1861 gcatcctcca caatttaccg cgggtttgct tgcatatcgc cttttcacac tggaaggcat
     1921 gtctgtttcc ggatttaccc tgagggcgaa caatcttaaa aaatgtttag attcggtcgc
     1981 ttgatgataa tgccaaactc caaatgacta atcttttgtc aagcacaatg ttctcgggct
     2041 tgaccacgtg gcggaagtcg ggggcctgat gctcaggctg agcttggagc tggatggtta
     2101 agcgggcagc aaaaagagaa ggcccgatcc ccgaggtgga gctcggaaag accaggccgc
     2161 atttctggag ctaaatgggg gactcgaacc cccgacctgc tgattacgaa tcagctgctc
     2221 taccaactga gctaatttag ctaatctctg atcagccgga agccgcaagg ctgggctgga
     2281 atctctacat aggtgttttt gtgggtgcgg gcatactgtt tgtccagctt ggaggcaaaa
     2341 ctgttgccca cgcgcaggct gatataaggc gtggtggtca tggtgacctt cacggtgctt
     2401 tccctgctga tgttgcgggg gtaccagaaa tccgcggcgt ccagcccggt ggtgagtttg
     2461 ttggattcat ccaggcggaa aggcggcacc tcaatatagt accagatgcg gaaatgggga
     2521 gtttcgggat attttttcag actgttcaga tggatcccga aaaagcgggc gggactgttc
     2581 cagaccagta attccggctg gggaaggtga ggctcaaaat gctccggctg gaggttgttt
     2641 gcccaatagt gggcttcaaa actgcctgag accgtgattg cctgtatctg ggcgtccttg
     2701 tgcaggaaaa ttccatagta atagtcggga tcgaggctgc ttttgggaag ctcaaaatca
     2761 acctgcatat acaggcgctg aagctcggtg gagtagaaca tatccagctc gtattcgtct
     2821 gcttccaaga gtttaaatct caatgcggca gtgctttcct gcgccataag gccagccgcg
     2881 agcaggaata gtaagacgaa catgaaatat tttctgcgca tgataaactc cttttataca
     2941 tagtttagct gtttcaatca gaaatacaag cgggatttag tcaagctatt tcttgtcgtt
     3001 caatccccct gactgccaaa attttccctt gacgcgaaag gctctcccaa aaatctgcct
     3061 ttactggaat att
//
LOCUS       DAOOWR010000204         3065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_31955_length_3065_cov_3.604651, whole genome shotgun sequence.
ACCESSION   DAOOWR010000204 DAOOWR010000000
VERSION     DAOOWR010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31955_length_3065_cov_3.604651"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /gene="recG"
                     /locus_tag="PLB85_05465"
     CDS             complement(<1..1767)
                     /gene="recG"
                     /locus_tag="PLB85_05465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU72814.1"
                     /translation="MEKAEFVKDTRTPVKFIKGVGEVRSRQLAKLGINTVLDLMEHFP
                     RAYIARKLNPSLRELKPGDNIALTAMISWVDERRTRKGKNLLEVGISDGRAAIVCSWF
                     SYPKSFPGLFKPGAMLWVAGNLTEYNGQLQLIHPEVEFVDDSEELDFWKKREFLPVYR
                     LSGDLTQKFLRRIVYSAFELFTSQIEENLPEELIAKRGFLPRKVALQKMHFSLHPEEL
                     DKVRKRFVYEEFFYSQLLWARHKRFHAQDVKGIRFENRRDLTSRLYRELPFKLTQAQK
                     RVINEIFGDMCSDRQMSRLLQGDVGSGKTIVTLFAMLLAVENGLQAALMAPTEILADQ
                     HYGNITRLLKDFPLNVVLLKGGSYKGKAQTKNAISTGEANLIIGTHALLQSDISFEKL
                     GFVAVDEQHRFGVQQRATLARKDKHPDLLYLSATPIPRSLSLTVYGDLEVSIINELPP
                     DRIPVKTLVRSNQKIDLVYREVAKDLAAGSQVYVVCPLIEESDKMDLLDAQRMHNHLS
                     TNIFPQYSCVLLHGKMPSREKDAIMLRFKAGEIRILVSTTVIEVGLDIPNASVMIVEH
                     AERFGLAQLHQLRGRVGRGGQQA"
     gene            2007..>3065
                     /locus_tag="PLB85_05470"
     CDS             2007..>3065
                     /locus_tag="PLB85_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOU72815.1"
                     /translation="MKVFKRIFTLLAVLSLMSCAVAQTKDLRSDDVPRFRQTAGSSAT
                     VWGSEPNPVVEVVRQVRQSVVQIKVESKVTVQNFRNPFFDDDFFRYFFPQQPRETQRP
                     VTSMGSGFVYEYNPQTREAFIMTNNHVVEKGKEGTITVTLADKVNYTATVVGLDPNTD
                     VAVIKIIVKEGEKITIAPLGDSANLEIGSWAIAIGNPFGDVGLDRTVTLGVISAIGRS
                     NLNFGSNSPIYQDYIQTDAAINPGNSGGPLLNIHGEVIGINSAITSTSGGNIGIGFAI
                     PVNLAKRVVEDLVANGKVTRAYIGIAPQEITADLMEALNLPEVSGVLVAKVEIDSPAH
                     KAGLSVGDVILEFNGEKVP"
BASE COUNT          664 a          863 c          855 g          683 t
ORIGIN      
        1 tgcctgctgt cctccgcgcc ccacccggcc acgcaactgg tgaagctggg ccaggccgaa
       61 gcgttcagcg tgttccacga tcatcacaga ggcgttgggt atgtccagcc ccacctcgat
      121 caccgtggtg gaaaccagaa tccggatctc gccggctttg aagcgcagca tgatagcgtc
      181 cttctccctg gagggcattt tgccgtgcag cagcacacag ctatactggg ggaagatatt
      241 ggtggaaaga tgattgtgca tcctctgggc gtcgaggagg tccattttgt cggattcctc
      301 gatgaggggg cagacaacat agacctggct gcccgcagcc aggtcctttg ccacttcgcg
      361 gtaaacgagg tctatctttt ggttagagcg gaccagggtt ttcacgggaa tcctgtcagg
      421 tggcagttcg ttgatgatgc tcacttcaag atcgccatac acggtgaggg agagggatct
      481 gggaattggt gtggcactga ggtagagcag gtccggatgc ttgtccttcc tggcaagggt
      541 ggcacgctgc tgcacgccga agcggtgctg ttcgtccaca gccacaaagc ccagtttctc
      601 aaaggaaata tcactctgca agagggcgtg ggtgccgatg atgaggttgg cttcacctgt
      661 ggaaatcgcg tttttggttt gggctttgcc tttgtaggaa ccgcccttga gcagcactac
      721 attcagggga aaatctttca gcagacgggt gatgttgcca taatgctggt ccgccagaat
      781 ctccgtgggc gccatcagag cggcctgtaa accgttttcc accgccagca gcatcgcgaa
      841 gagggtcacg atcgtctttc cggaacccac atcgccctgc agcagcctcg acatctgcct
      901 gtcggaacac atatctccaa agatttcgtt gataacgcgt ttctgggcct gggtgagttt
      961 gaacggcagt tcgcggtata gcctggaagt aagatcccgc ctgttttcga agcggatgcc
     1021 cttaacatct tgggcgtgaa agcgcttgtg gcgcgcccag agcaactggg aatagaagaa
     1081 ttcctcatag acaaagcgtt tgcgcacctt gtccagttcc tccgggtgca gggagaaatg
     1141 catcttctga agcgcgacct tgcggggcag gaaaccgcgt ttggcgatca gctcttccgg
     1201 aaggttttcc tcgatctggg aagtgaaaag ctcgaaagcg ctgtaaacta tccggcgcag
     1261 gaacttctgg gtaaggtcgc cgctgagcct gtaaaccggc agaaactcgc gcttcttcca
     1321 gaaatccagc tcttcgctgt cgtccacgaa ttcaacttca ggatgtatta gctgcaattg
     1381 gccattatat tcggttaagt ttccagcaac ccagagcatg gcgcccggct tgaacagccc
     1441 cggaaaactt tttggatagc tgaaccagga acagacgatg gcggcgcggc cgtcgctgat
     1501 gcccacctcg agcaggtttt ttcccttgcg ggtcctgcgt tcgtccaccc aacttatcat
     1561 cgccgtaaga gcaatgttgt cgccgggctt gagctcccgc agagagggat tcagcttgcg
     1621 ggcgatataa gcccggggga aatgctccat caggtccaag actgtattga tgcccagttt
     1681 ggccagttgg cgcgagcgga cctcgcccac ccccttgatg aatttcactg gggtgcgtgt
     1741 atcctttaca aattcagctt tttccatgtg agggccattc tccccgaggg taaaatgttg
     1801 tcaatatccg cgtcggggat ttcgcctttc ccaaaaacag gctggcttgg gaggcggagg
     1861 gcaccgcggc aattttttcc ttgaccgcgg cgttggggtc gattattatg ttcgggaaca
     1921 gcacttgaag cgctatgtga ttggttttta tttttttaca ccccaatatg cccgggaact
     1981 gttggtttcc ggtttcagga gaaaagatga aagtattcaa acgtatattc accctgttgg
     2041 cggtcttgag cctgatgtcc tgcgccgttg cccagaccaa agacctccgc tcagacgatg
     2101 tgccccggtt ccgccagact gccggcagca gcgccactgt ttggggcagt gagcccaatc
     2161 cagtggtcga ggtggttcgc caggtgcgcc aatccgtggt gcagatcaag gtcgaatcca
     2221 aggtcaccgt ccagaatttc agaaatccct tcttcgacga cgatttcttc cgctacttct
     2281 ttccccagca acccagggag acgcaacgcc ccgtaacctc gatgggcagc ggcttcgtgt
     2341 atgaatacaa tccccaaacc cgcgaagcct tcatcatgac taacaaccac gtggtggaga
     2401 aaggcaagga aggcaccatc accgtcaccc tggccgacaa ggtcaattac accgccacgg
     2461 tggtgggact ggaccctaac accgatgtgg ccgtgatcaa gatcatcgtc aaggaagggg
     2521 aaaagatcac cattgccccg ctgggcgact ctgccaacct ggaaatcggc tcctgggcca
     2581 tcgccatcgg aaatcccttt ggcgacgtgg gcctggaccg caccgtaacc ctcggcgtga
     2641 tctcagccat cggcagaagc aacctcaatt tcggcagcaa ttcccccatt tatcaggact
     2701 acatccaaac cgacgccgcc atcaaccccg gcaacagcgg cggccccctt ttgaacatac
     2761 acggcgaggt gataggcatc aactccgcca tcaccagcac ctccggcggc aacatcggca
     2821 tcggcttcgc gattccggtg aacttggcca aacgagtggt ggaagacctt gttgccaacg
     2881 gcaaggtgac ccgagcctac attggcatcg cgccccagga aatcaccgct gacttgatgg
     2941 aagccctcaa cctgcctgag gtttcgggag ttctggtggc caaggttgaa atagattcac
     3001 ccgcccacaa ggccggcctc tccgttggcg acgttatttt ggaattcaac ggtgaaaagg
     3061 tgccc
//
LOCUS       DAOOWR010000205         3055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_32079_length_3055_cov_3.961333, whole genome shotgun sequence.
ACCESSION   DAOOWR010000205 DAOOWR010000000
VERSION     DAOOWR010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32079_length_3055_cov_3.961333"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2016)
                     /locus_tag="PLB85_05475"
     CDS             complement(43..2016)
                     /locus_tag="PLB85_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOU72816.1"
                     /translation="MSGLSFLNSGLLLFTAATVLPLLIWLLAKKKPKQVVFPSLRFIK
                     AGQEEEKKRTRLKNILLLIIRMLIILLVTLAATRPLLRSSRLKPSSSHPPTAIAILVD
                     TSYSMDYVEESRSDLDRAKDALKKIAALCDPEDRVILVTSEDKWNRLHAQIYAGSPPP
                     ELIDQIATTYTPLPLQNMLELAEAKLAETQLANRELYFITDGQKQDYPSSPETRLNVI
                     NIAPNREPANLSCANARALPQLVEKSRRQTIEFQLTNHGNQPREEVLVKVVLGDAKVA
                     EKFVALPARQTVNQSIAIDLRQDGWQSGYVEVVDDRLEHDNRSYFSFPFQLSPRVAVI
                     SGAQGLPLSLRSLLSVYTGSGGSVDRLDPSQLNLSLLDSYQTFVFVGAVPYSPKLRDI
                     VAKLGESGRGALFCLDGTLSAEYRSLLAGTFGISFGTWQSEPKTISHVNPHHYTTSLL
                     AGKNIRYNTLAKYWKLSGTGATTLLSGGGDIFALAKDNFVLWNFDPGDQHSTFLLDAA
                     FAVFAYRCLEHAGTLIASGENYLLGYTVNASGIVLPNGQSIQLASRTHLLEQPGIYSL
                     LQAEGPAKVVAVNAPLQESEFQAMDYKGLSNTRLLGPNWIKTLFHTRLGHDLWKILLA
                     AALALVILELVLVKSEELRPATAVPNPATGSET"
     gene            complement(2020..>3055)
                     /locus_tag="PLB85_05480"
     CDS             complement(2020..>3055)
                     /locus_tag="PLB85_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72817.1"
                     /translation="TLLIFTITLLCVELAAYGEVLPITSFDYGTPENSVSPIAIGMGS
                     MNVTNASDFYASFSNPALLGGNEYSLASASFRVKGDEAMTIADAVQFSNVLRPSQFKY
                     VTLVAKQTAFTYQPVASVNIVESPDPAWPNNPSGYRYLDYQLDKWQISLAGSDNSWRP
                     IRFGLNAKYLSGRLVYMRSARPDTTFIDTKVKGFSTDLGLTLQTGSFTIGLVSTDLLS
                     RLWWDWGYESVPLQSRMAFGAQYGTEYLTVSGGVLSRLSRNPDTTYHLGFQYLWDWQG
                     TSSEPNNSQAFTARIGLYSRDFNGTDNINYTLGAGYNYKIFRFDFALNNKGFKLSDSE
                     YLFSLGVGLP"
BASE COUNT          639 a          769 c          954 g          693 t
ORIGIN      
        1 tgaagtcttt tattacggat gggtctttaa tcttttccaa tatcatgttt cactcccggt
       61 tgcggggttg ggcaccgcgg ttgctggacg aagttcctcg cttttcacga ggaccagttc
      121 caggatcacc agcgccagcg cagcggccag caatatcttc cagaggtcat gtcccaagcg
      181 ggtgtggaag agggttttga tccagttcgg ccccagtagc ctggtattgc ttaggccttt
      241 gtagtccatt gcctgaaact cgctctcctg caggggggcg tttaccgcga ccactttggc
      301 ggggccctca gcctggagta gcgagtagat gcccggctgt tccaaaaggt gggtcctgct
      361 ggccagctgg atgctttgcc cgttgggaag gacgattccg ctggcgttca cggtatatcc
      421 cagcaggtag ttttccccgc tggcgatgag agtgccggca tgttccaggc agcgataggc
      481 gaaaaccgcg aaagcggcgt ccagcaggaa ggttgagtgt tggtcgccgg ggtcgaaatt
      541 ccaaagcacg aagttgtctt ttgccagggc aaagatgtcc ccgcccccgc tcaacagggt
      601 ggttgcgccg gttcctgaga gcttccagta tttcgccagc gtgttgtagc ggatgttttt
      661 tcccgccaac aacgaggtgg tgtagtgatg gggattcacg tggctgatgg ttttgggctc
      721 agattgccac gtgccaaagc taatgccgaa ggttcccgcc agcagagagc ggtattccgc
      781 ggaaagggtg ccgtccaggc agaaaagcgc gccgcggccg ctttcaccga gttttgctac
      841 gatgtcgcgc agttttggtg aatagggtac cgctcccaca aacacaaaag tttggtagct
      901 atccaacagg gaaagattga gttggctggg atctagccgg tcaacgctgc ccccgctgcc
      961 ggtatacaca ctgagcaacg acctcaggga aagaggcagg ccctgtgccc cggaaatcac
     1021 ggccacccgg ggtgaaagct ggaaggggaa agaaaaatag ctgcggttat catgctccaa
     1081 acggtcatcc accacttcca catagccgct ttgccagccg tcctgcctca ggtcgatggc
     1141 gatgctttga tttacggtct gcctggctgg caaagccacg aatttctccg caaccttggc
     1201 atcgcccaga accactttca ccagcacctc ctcccgcggc tggttgccgt ggttggtgag
     1261 ctggaattca atggtctggc ggcgggattt ctccaccagt tgtggcagcg cgcgggcgtt
     1321 ggcgcagcta aggttggcag gttcccggtt tggggcgatg ttgatcacgt tgagccttgt
     1381 ttccggcgag gaggggtagt cctgtttctg gccgtctgtg ataaagtaga gttcgcggtt
     1441 cgcaagctgg gtttcagcca gcttggcttc ggcgagttcc agcatgttct gcaggggcag
     1501 gggagtatag gtggttgcaa tttggtcgat aagttcgggg ggcgggcttc cggcatagat
     1561 ctgggcgtgg agacggttcc atttgtcctc ggatgtgacc aggatcacgc ggtcctcagg
     1621 gtcacaaagg gcggcgatct ttttcagagc atccttggcc cgatccaaat cgctgcgcga
     1681 ttcctccaca tagtccatgc tgtaggaagt gtccaccagg atcgcgatgg cagtgggagg
     1741 atggctggat gacggtttga ggcgtgaaga gcgcagcagc ggacgggtgg cggccagggt
     1801 caccagcagg atgatgagca tacggatgat gagcaggagg atgtttttta ggcgggtgcg
     1861 ctttttttcc tcttcctggc ccgctttgat gaagcgcaag gagggaaaga ccacctgttt
     1921 gggcttcttt ttggccagaa gccagatcag cagtggcaaa acggtggccg ccgtgaaaag
     1981 caacaagccg gaattgagaa agctgagccc gctcatggat tatggcaatc ctactcccaa
     2041 ggaaaaaaga tactcgctgt cgctcagttt gaagcctttg ttgttcaagg caaagtcgaa
     2101 gcggaaaatc ttgtaattat aaccggcgcc gagggtgtag ttgatattgt cggttccgtt
     2161 gaagtcacgg ctgtaaaggc ctatcctggc tgtaaaagcc tgggaattgt ttggctcgga
     2221 agatgtgccc tgccagtccc agagatactg aaagcccaga tggtaagtgg tgtccggatt
     2281 cctggacagc cggcttagaa caccgccgct gacggtgagg tattccgtgc cgtattgcgc
     2341 cccgaaagcc atccggctct gcaagggaac ggattcgtag ccccagtccc accacaggcg
     2401 ggagagcaga tcggtggaaa ccagaccgat agtgaaactc ccggtttgca gggtcagccc
     2461 cagatcggtg gaaaaacctt tcactttggt atcgatgaac gtggtgtccg gcctcgcgga
     2521 acgcatatac accagccggc ctgacagata tttggcgttc agtccaaagc ggattggccg
     2581 ccaggaattg tcgctcccgg caaggcttat ctgccacttg tccaactgat agtccagata
     2641 gcggtaacca gatgggttgt tcggccaggc gggatcaggg ctttcgacga tgttgacgga
     2701 ggctacgggc tgataggtaa aagcggtttg tttggctacc agggtgacgt atttgaactg
     2761 gctgggcctc aacacgttac tgaattggac tgcatccgca atggtcatgg cctcgtcgcc
     2821 cttcaccctg aacgaagcag aggcaaggct gtactcgttg ccgccaagca gggctggatt
     2881 tgaaaagctg gcgtagaaat cactggcgtt ggtcacgttc atgctgccca taccgatggc
     2941 gatgggggaa acgctgtttt ccggggttcc gtagtcgaaa gaggtgatgg gcagaacttc
     3001 cccgtaggca gcaagttcca cgcagagcag ggttatagtg aaaataagaa gggtc
//
LOCUS       DAOOWR010000206         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_32134_length_3052_cov_4.066400, whole genome shotgun sequence.
ACCESSION   DAOOWR010000206 DAOOWR010000000
VERSION     DAOOWR010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32134_length_3052_cov_4.066400"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /gene="lgt"
                     /locus_tag="PLB85_05485"
     CDS             <1..487
                     /gene="lgt"
                     /locus_tag="PLB85_05485"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU72818.1"
                     /translation="LIYMKRQKLSFFKLADPAMPLVAVGLGLGRLGNFINAELWGKVT
                     TLPWGMVFPGTDAGSLPRHPTQLYEMFLEGVVLFTVSFILLKKSRREGIVFWTFIGLY
                     GIFRFLVEFVREPDQLDIYDKYGYFLGFMTIGQILSLLMVIAAAIGIWKLYQKKPEAK
                     P"
     gene            484..1140
                     /locus_tag="PLB85_05490"
     CDS             484..1140
                     /locus_tag="PLB85_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72819.1"
                     /translation="MKRLIPVLLIVSALLLSSCTSIKPVDKLSLLRAELARWQNFSAD
                     GIVRVTYAGLTLHKMFVLAKSVDSARLDIVDGGAFGISPSPLISVYLADYLAMESAFL
                     KLPDFSKAQMDTSSYLALLADPEALLAKYGDEIAANEEVSAGDLRVTFTPKMQLAKVE
                     DATSGAVVSITYNSKGDPDKVLFRIDSNKSVELLVDSISYGEAEAVPLPRPEESSEQP
                     "
     gene            1137..2432
                     /gene="purB"
                     /locus_tag="PLB85_05495"
     CDS             1137..2432
                     /gene="purB"
                     /locus_tag="PLB85_05495"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU72820.1"
                     /translation="MISRYCLPEMERIWTQQNRYECWLEVELAAARAMFELGIIPEED
                     WRSISEKADFSCERIDEIEAVTRHDVIAFLTNVAEYVGEPARWIHFGLTSSDTLDTAT
                     ALQLKQSGELILTELHRLAETLKLKAREHRNTICMGRSHGIHAEPTCFGLKFALWHDE
                     TKRNIERMQNAIETVAVGQFSGAVGNFAHLDPQVEELACKHLDLRPANVSTQVLQRDR
                     HAFFLGELALIGSLVEKIALEIRHLQRTEVQEAEESFAAGQKGSSAMPHKRNPIQSEQ
                     FCGLARLLRSNAQAALENNALWHERDISHSSVERVILPDSCILTHYMLAKCCRLISNL
                     VVYPKNMKANLELTNGLVFSQAVLLHLVKQGLPREEAYALVQEAAMQCWESGTPLLDH
                     ILANSRITALLPASEIKDIFSYDRYLRHVDAIFKRCGII"
     gene            2464..2904
                     /locus_tag="PLB85_05500"
     CDS             2464..2904
                     /locus_tag="PLB85_05500"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOU72821.1"
                     /translation="MKKLIFVATLLAMLLPIALIAQTYNIEGLWYVQDKAGKIQIYKN
                     SSGSYEGKIVWQKEGFEDGKPKMDKKNPDKSLRSRPLTNLVVIKNLKSQGNNKYDGGT
                     IYDLTTGNTYSVKVELTGPNTMKLRGFIGFSLLGKTLTWTRASS"
BASE COUNT          741 a          914 c          754 g          643 t
ORIGIN      
        1 cctcatctat atgaagcggc agaaactcag tttcttcaaa ctggccgatc ccgccatgcc
       61 tctggtggca gtaggtttgg ggctcggacg cctgggtaat ttcatcaacg cagagttgtg
      121 gggaaaagtg accaccctgc cttggggcat ggtattcccc ggcaccgacg ccggctctct
      181 gccccgccac cccacccagc tctacgagat gtttctggaa ggggtcgtgt tgttcacggt
      241 cagctttatc ctgcttaaaa aaagccggcg ggaaggaatc gttttctgga ccttcatcgg
      301 cctttacggg attttccgct ttttggtgga attcgtacgg gagccggacc agcttgacat
      361 ttatgacaaa tatggttatt tcttgggctt catgaccata ggccagatcc tcagcttgct
      421 gatggtgatc gctgctgcca tcggcatctg gaaactttac caaaagaaac cggaggccaa
      481 gccttgaaac gtcttatccc cgtcctgctg attgtcagcg cgctgctgct aagctcctgc
      541 acctcaatca aacccgtgga caagctttcc ctgctgcgcg ccgaactggc ccgctggcaa
      601 aacttcagcg ccgacggcat cgtgcgcgta acctatgcag gactaaccct gcacaagatg
      661 ttcgtgctgg ccaagtccgt cgattccgcc cggctcgaca ttgttgacgg cggcgcgttc
      721 gggatttcgc cctctccatt gatttcggtc tatctggcgg attatttagc catggaatca
      781 gcgttcctca agctgcccga tttttccaaa gcacagatgg acacttcctc ttatctggct
      841 ttgctggccg atcccgaagc cctgttagcc aaatacggag acgagatcgc agccaatgaa
      901 gaagtatctg caggcgacct tcgtgtaaca ttcaccccca agatgcagct ggccaaggta
      961 gaggatgcca cttccggtgc cgtggtgtcc ataacctaca attccaaagg cgaccccgat
     1021 aaagtgctgt tcaggatcga cagtaacaaa tcggtggagc ttttggtgga cagcatcagc
     1081 tatggtgaag cagaagccgt tcctttgccc cgtcctgaag agagttctga acagccatga
     1141 ttagccgcta ctgcctgccg gaaatggaac gcatctggac ccagcaaaac cgctatgaat
     1201 gctggctgga ggtggaactc gccgccgccc gggccatgtt tgaactgggc atcataccgg
     1261 aagaggactg gcggtctatc tctgaaaaag ccgatttttc ctgcgagcgt atagatgaga
     1321 tcgaagccgt cacccgtcac gacgtgatcg cttttctcac caatgtggcg gaatatgtgg
     1381 gcgaaccagc ccgctggatt cacttcggcc ttacctcctc agacaccctg gataccgcca
     1441 ctgcgctaca actaaagcaa tccggagaac tgatcctaac agaactgcac cgcctggctg
     1501 aaaccttgaa actgaaagcc cgcgaacacc gcaacaccat ctgcatgggc cgttcgcacg
     1561 gcatccatgc tgaaccgact tgtttcggac tcaaattcgc cctctggcat gacgagacca
     1621 aacgcaacat cgaaaggatg caaaacgcca ttgaaactgt ggccgtaggc cagttcagcg
     1681 gcgctgtggg caacttcgcc catctcgatc cccaagttga ggaactggcc tgcaagcatt
     1741 tggacctgcg ccccgccaac gtttccaccc aagtgctgca gcgtgatcgg cacgcctttt
     1801 tcctcggtga acttgccctc atcggcagcc tcgtggaaaa aatcgctttg gaaatccgcc
     1861 accttcagcg cactgaggtg caggaagcgg aggaaagctt tgccgcaggc cagaaaggct
     1921 cttccgcgat gcctcacaaa cgcaatccca tccaaagcga acagttttgc ggcctggccc
     1981 ggctgttgcg ctcaaacgcc caagccgcgc tggaaaacaa cgccctctgg cacgagcgcg
     2041 acatctccca ttcgtcggtg gaacgcgtga tccttccaga ttcatgcatc ctcacacatt
     2101 acatgctggc caaatgctgc cgcctcattt ccaacctcgt cgtttacccc aagaacatga
     2161 aggccaatct ggaactcacc aacggcctcg ttttctccca ggcagtgctg ttgcatctcg
     2221 tgaagcaagg cctgccccgt gaggaagcct acgccctggt gcaggaagcc gcaatgcagt
     2281 gctgggaaag cggcacaccc ctgcttgacc acattctggc gaacagccgg atcaccgcgc
     2341 tgctcccggc ttccgagatc aaggacatct tttcttacga caggtatcta cgccacgtag
     2401 atgccatttt caaacgctgc gggatcattt gaacccgccc aaaacccaga taaggagaac
     2461 aagatgaaaa agctgatatt cgtcgcaacc ctgctggcaa tgctgctccc catcgccctg
     2521 atcgcccaaa cctacaatat agaaggcttg tggtatgttc aggacaaagc gggaaagata
     2581 caaatctata aaaactcgtc aggaagctat gaaggcaaaa tcgtctggca gaaagagggc
     2641 tttgaagacg gcaagcccaa aatggacaag aaaaaccctg acaaaagcct ccgttcccgc
     2701 ccactgacca acctcgtggt catcaagaac ctcaaatcac agggcaacaa caaatacgac
     2761 ggcggcacca tctacgacct cacaaccgga aacacctatt ccgtaaaggt ggaactcacc
     2821 ggccccaaca ccatgaaact ccgtggtttc ataggctttt ccctgctggg caaaacgcta
     2881 acttggaccc gcgcctcatc ctgaaccatt ttcgcgaaga gctgccagga tcgcaagatc
     2941 ctggctggtt cttcagcacc tgaactttga tagaccagcc aggccggagc ctgggacaag
     3001 aggaatgaat gcctgatacc acaagcaaat acctgctctc cgatgcgaac tc
//
LOCUS       DAOOWR010000207         3041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_32306_length_3041_cov_3.762224, whole genome shotgun sequence.
ACCESSION   DAOOWR010000207 DAOOWR010000000
VERSION     DAOOWR010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32306_length_3041_cov_3.762224"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLB85_05505"
     CDS             <1..297
                     /locus_tag="PLB85_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU72822.1"
                     /translation="FLFGGLPGTGYSLTLPIFHLFSGGLLLGAFFMATDYTTSPITKN
                     GRVVYAIGCGLLTVIIRLVGGYPEGVSYSILFMNVMTPLIDILTRPKAFGRSKK"
     gene            294..860
                     /locus_tag="PLB85_05510"
     CDS             294..860
                     /locus_tag="PLB85_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOU72823.1"
                     /translation="MKMFLKLGLILLAFCVVATALLAYVNSVTKPKIDELKVKEAEEA
                     RSSLIPGSTFEEQSFKAGEESHTYYVAKDTTTGQIKGYTFTAAKNGYSSNVKTMAAID
                     PDFKLIAIKVIEQAETPGLGANSTQSLFTDQFKGLAADQLLVDKDGGPIKALTGATIT
                     SRAVANSLKEQITLLRADIESRQGEAQP"
     gene            857..1450
                     /locus_tag="PLB85_05515"
     CDS             857..1450
                     /locus_tag="PLB85_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOU72824.1"
                     /translation="MSFIKELTKGIIKENPTFVIVLGMCPTLATSTSVNNAVGMGVAA
                     TFVLVCSNIMISLIKNITPDKIRIPVYVVVIAAFVSVVDMAMAAFIPDLHKSLGLFIP
                     LIVVNCIILGRAEAFANKNNVLNSMADGIGMGLGFTLSLSVMATFREILGNGTWAGMR
                     VMPHSYNPMLIAILAPGAFFTLGLLMATINLLKGRKQ"
     gene            1447..2049
                     /locus_tag="PLB85_05520"
     CDS             1447..2049
                     /locus_tag="PLB85_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOU72825.1"
                     /translation="MNFLGQLFVMAVSAIFIQNFVLSRFLGLCPYLGVSKKMDSAFGM
                     GMAVIFVMTMASAICFLIYKYLLVPYGLTYLSTLAFILTIAALVQFVEMVIQKNAPAL
                     YKALGVYLPLITTNCAVLGVAILNITPLADGSTYNFLFATLNGFLSGVGFTFVLLAMA
                     GIRQRLELVDMPKSMEGFASGMILAGTMALAFYGFMGLKI"
     gene            2069..>3041
                     /locus_tag="PLB85_05525"
     CDS             2069..>3041
                     /locus_tag="PLB85_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOU72826.1"
                     /translation="MTPFIILAASMTSQIVTPVIILGAMGLIFGLVLAFAAKVFTVTV
                     DPRVEQIISVLPGANCGACGSAGCSGYADSVVNGGAPCNLCAPGGAAVAKAIAGIMGT
                     EAGAVEKKVAVIHCSSGGRDNTKWKYAYKGIESCLSAASIAGGPNSCSWGCIGFNDCV
                     KACMFGAISIDESGMRVIDPEKCTACGACVKACPRKLIELVPLKNSVHIKCSSQDKGP
                     LARQSCGNEKPCLGCGICSRKCPVQAITIENNLARIDYAKCVNCGICATVCPSGAILD
                     LLGGRRKKAEINGELCIGCTICAKSCPVEAISGEVKQAHKIDRGKCVG"
BASE COUNT          669 a          899 c          803 g          670 t
ORIGIN      
        1 ttcctgttcg gcgggttgcc cggaactggc tattcgctta ccctgccgat ctttcacctt
       61 ttctccggcg gactgctgct gggcgctttc ttcatggcga cggactacac caccagccca
      121 ataaccaaaa acggcagggt cgtttatgcc atcggttgcg gcttgctcac tgtgataatc
      181 cgtttggtgg gtggatatcc tgaaggggtt tcctactcca ttctgttcat gaatgttatg
      241 acccccctca tcgacattct cacccgtccc aaagccttcg ggaggtccaa gaaatgaaaa
      301 tgttcctcaa actcggcctc atcctgctgg cgttctgcgt tgtggccacg gctcttctgg
      361 cctatgtgaa ttcggtaacc aaacccaaaa tcgacgaact gaaggtaaaa gaagccgagg
      421 aagccagaag ttcgctgatc ccgggctcga cctttgaaga gcaatccttc aaagccgggg
      481 aggaatccca cacttattac gtggccaaag acactacaac cggccagatc aaaggctaca
      541 ccttcaccgc tgccaaaaac ggatattcaa gcaacgtgaa gacaatggcc gccatcgacc
      601 ctgattttaa gctgattgcc atcaaggtga tagagcaggc cgaaaccccc ggcctcggcg
      661 cgaacagcac ccaaagctta ttcacagacc agttcaaagg tctggcagcc gaccagttgc
      721 tagtggacaa ggacggcgga ccgatcaagg cgctgacagg cgccaccatc acttcccgcg
      781 cggtagcgaa ctcgctgaag gagcagataa ccttgctgcg cgcagacatt gaatcccggc
      841 aaggggaggc gcaaccatga gcttcataaa agaacttacc aaggggatca tcaaagaaaa
      901 tcccaccttc gtcatcgtgc tgggcatgtg ccccaccctg gcgacatcga cttcggtgaa
      961 caacgcagtc gggatgggcg tcgcggccac tttcgtgctg gtttgctcga acatcatgat
     1021 ctcgctgata aagaacatca cgccggacaa aatccgcatc cccgtctatg tagtcgtgat
     1081 agcggccttc gtgtcagtcg tggacatggc gatggcggct ttcattccgg acctgcacaa
     1141 gtccctgggc ttgttcatac ctcttatagt ggtgaactgc atcatcctgg gccgcgctga
     1201 agccttcgcc aacaagaaca acgttttgaa ctccatggcc gacggaatcg gcatggggct
     1261 gggcttcacc ctttctctct cggtgatggc cacgttccgc gaaatcctgg gcaacggcac
     1321 ctgggccggg atgagggtaa tgcctcatag ctacaatcca atgctgatag ccattctcgc
     1381 gcctggagcc tttttcaccc tgggcctgct gatggcgacg atcaatctgc tgaaggggag
     1441 gaaacaatga actttctggg ccaacttttc gtgatggcgg tgagcgccat cttcatccag
     1501 aacttcgtgc tgtctcggtt tttgggactc tgcccctatc tgggagtttc caagaaaatg
     1561 gattccgcct tcggtatggg catggccgtt atctttgtga tgaccatggc ttcggccatc
     1621 tgcttcctga tttacaaata cctgctggtg ccatacgggc tcacctatct gagcacgctg
     1681 gccttcatcc tcaccatcgc ggccctggtg cagttcgtgg aaatggtcat ccagaaaaat
     1741 gcccccgccc tctacaaagc tctgggcgtc tatctgcctc tgatcacaac caactgcgcc
     1801 gttctgggag tggccatcct gaacatcacc ccgcttgcag acggctccac ctacaatttc
     1861 ctctttgcca ccctgaacgg attcctttcg ggcgtgggct tcacctttgt cctgctggca
     1921 atggccggca tccgccagcg tctggaactg gtggacatgc ccaaaagcat ggaaggtttc
     1981 gccagcggca tgattctggc cggaaccatg gccctggcct tctacggctt catggggctg
     2041 aaaatctgag gaagggaaaa tgacgccaat gacaccgttc atcatcctcg ccgcctccat
     2101 gacatcccag atagtgaccc cagtcatcat tttaggtgcc atgggcctga tctttgggct
     2161 cgtcctggct ttcgccgcca aggttttcac cgtgactgtt gacccccggg tggagcaaat
     2221 catttccgtg cttcctggcg ccaattgcgg tgcctgtgga tccgcaggtt gctctggcta
     2281 tgccgattcc gtggtcaacg gaggagcgcc ctgcaacctt tgcgcccctg gaggcgccgc
     2341 tgtcgccaag gccatagccg gcatcatggg cacggaagcc ggcgcggtgg agaaaaaagt
     2401 cgcggtgatc cactgcagtt ccggtggccg cgacaatacg aagtggaaat atgcctacaa
     2461 gggcatcgaa agctgccttt ctgcggctag cattgccggc ggcccgaact cctgttcctg
     2521 gggctgcatt ggcttcaacg actgcgttaa agcctgcatg tttggcgcca tcagcattga
     2581 tgaaagcgga atgcgcgtga tagacccgga aaaatgcacc gcctgcggag cctgcgttaa
     2641 agcctgcccc cgcaagttga tagagttggt tccgcttaaa aactccgttc atatcaaatg
     2701 ctcctcccaa gacaagggcc cccttgccag gcaaagctgc ggcaacgaaa aaccctgcct
     2761 cggctgcgga atctgttcac gcaaatgccc ggtgcaggcc atcaccatcg aaaacaacct
     2821 cgcccgcatc gactatgcga aatgtgtcaa ctgcggcatc tgcgctacag tctgccccag
     2881 cggtgctatt cttgacctgc ttggcgggcg ccgcaagaaa gctgaaatca acggtgaact
     2941 ctgcatcggc tgcaccatct gcgcgaaaag ctgtcccgtg gaagccatca gcggagaagt
     3001 gaaacaggcg cataagattg acagaggcaa atgcgtcggc t
//
LOCUS       DAOOWR010000208         2998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_32889_length_2998_cov_3.497112, whole genome shotgun sequence.
ACCESSION   DAOOWR010000208 DAOOWR010000000
VERSION     DAOOWR010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32889_length_2998_cov_3.497112"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLB85_05530"
     CDS             <1..909
                     /locus_tag="PLB85_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU72827.1"
                     /translation="RQQAVEWFRRAANQNVNSAQYSLGVCFFMGEGIAQNQREAVKWY
                     DLAASGGNLNAMFSLARCYYYGTGVNQDKAAAVRIFRQAADKNHIPSINDLAFCYKYG
                     EGVQTNASESAAWYAKAAAAGDAFAQFNLAQAYAKGEGVPQNNEEALKWYRSAADRNY
                     PEAHYQIALTNLSGPGRNAGEAIKWLTSAAELGHREAQYQLGQIYFGGEMIPKDMTEG
                     AKWYLRAADQGHPSAQYEIGNCYAFGEGVKENATEAFFWYSLAAANGEADAEQARGLL
                     SSMFTPQDIDNLLLRAESWYRQHYSD"
     gene            1608..2180
                     /locus_tag="PLB85_05535"
     CDS             1608..2180
                     /locus_tag="PLB85_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72828.1"
                     /translation="MKNNSSGWAPTITLAKLFEEQNQFFEALSAYEMISQTDSSAAVR
                     ESIEALHQRILNDPANRYDPRIEKLFTPEELAYLRILDHQGFENLARAREKLNEGSLG
                     ADIYIEEDEDWLDGDEAETDALTQILQEIEQQTQLNLAETVPDAREFTVKDLLMALLS
                     RFDKDQKLTDVKLSDLVAVVLEMQEPKDKP"
     gene            2363..2905
                     /gene="rsmD"
                     /locus_tag="PLB85_05540"
     CDS             2363..2905
                     /gene="rsmD"
                     /locus_tag="PLB85_05540"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU72829.1"
                     /translation="MRIITGKYKGRNLLTVDGRTTRPTTAYNRAMIFNVYSDLAGKRV
                     LDLFAGTGFYGLEALSRGAEWVDLVEFATPAVAVILKNVALLGCGDDCHVWRKRADAF
                     LKGTDTRWDVIFLDPPYAKNLLNPCLDLIYQRGLLLPGGVAIAEHSPQEPVAEAYQEL
                     VLKVKTGRSSCFTLLRAEEK"
BASE COUNT          738 a          830 c          783 g          647 t
ORIGIN      
        1 aggcaacaag ctgtggaatg gttccgccgt gccgccaacc aaaacgtgaa ctcagcccaa
       61 tacagcttgg gagtgtgctt tttcatgggt gaagggatag cccaaaacca gcgtgaagca
      121 gtgaaatggt atgaccttgc tgccagcggc ggaaacctca acgccatgtt cagcctggcc
      181 cgctgctatt attacggcac cggggtcaac caggataaag cagccgccgt gcgcattttc
      241 cgtcaggcag ccgacaaaaa ccacatcccc tccatcaacg acctcgcctt ttgctataaa
      301 tatggagaag gggtgcaaac caacgcctcc gaatccgctg cctggtatgc caaagctgcc
      361 gcggccggtg acgcttttgc ccagttcaac cttgcccagg catacgccaa aggcgaaggg
      421 gtgccccaaa acaacgaaga agccctcaag tggtatcgca gcgcggcgga taggaattat
      481 cccgaagccc attaccagat cgcgcttacc aatctcagcg gacccggacg caatgccggc
      541 gaagccatca aatggctcac cagcgcggcc gaacttggtc accgggaagc gcagtatcag
      601 cttggccaga tctatttcgg cggggaaatg atccccaagg acatgaccga aggcgccaaa
      661 tggtatctcc gcgccgctga ccagggacat ccaagcgccc agtacgaaat cggcaactgc
      721 tacgctttcg gcgaaggcgt caaggagaat gccactgaag ctttcttctg gtattcgctg
      781 gcagccgcca acggcgaagc cgatgccgaa caggcccgcg gcttgctttc ctcaatgttt
      841 acacctcagg atatcgataa tcttctcctg agggcggaat cctggtacag acaacactat
      901 tctgattgag cccagaactt tacggacggg attccctgtc ctgcatattc gattttttaa
      961 gccggtttca aggccggctt tttactgccc tgatgccaaa ccatgttaag cagttgataa
     1021 tacgtcgtct aaaagacagg gaatatgaga aaggaaaatt tagcttgaca taaatcatag
     1081 tcttaacaat gtgacctata cagaccctac atagaatgcc ctccgagtcc gtggggacgc
     1141 cgcccggcga tccccacggt tttttctgct tggaaaacct ttcatgcttt gggttctctt
     1201 tcaaatcaaa cggctgttgt agcgcaatca gaaagcgtcc ctctgggctt tacccaccca
     1261 gtttgaaaaa gagccataca tatttaccta cctcaatgcc ctgtctgtca gaccctgagc
     1321 gctcatttcc atcccactcc catgcctccc gcctaatcct cgcaccaaat gcgggcataa
     1381 tacgggaggc gaatccggga ttcttgaaag ggccttaacg gccaaaggac tttgctccgt
     1441 tcaagatgtt tccgttcccg gcgaccatga aaccgaagcc tttcctttgg tacggaacgg
     1501 gaagcggttt tgatgtgttt ccaacgcctt tgacccggca aaggttttca ttgacacaaa
     1561 tgcgcccctg tccagattgg tgccgatgat tcaacaaggg agttcacatg aagaacaata
     1621 gctcaggctg ggcgccaacc atcactctgg caaagctttt tgaagaacaa aaccagtttt
     1681 ttgaggccct gtccgcttat gaaatgatca gccagaccga ttcttcggcg gccgtgaggg
     1741 aaagcattga agcgctgcat cagcgtattc tgaacgatcc cgcaaaccgc tatgaccccc
     1801 gcatcgagaa acttttcact ccggaggaac tggcttacct gagaatcctg gaccatcagg
     1861 gttttgaaaa cctggcccgg gccagggaaa agctgaatga aggctctttg ggcgcagaca
     1921 tctatattga ggaagatgag gattggttgg acggagacga agccgagacg gacgctttga
     1981 cgcaaatcct gcaggaaatt gaacagcaga cccaactgaa tctggccgaa acggttcccg
     2041 atgcccggga attcacggtg aaagacttgc tgatggcgct tttaagcagg tttgacaagg
     2101 accagaagct caccgacgtc aagttaagcg acctcgtggc agtagttctg gagatgcagg
     2161 agcccaaaga caaaccctga tgccggccaa agcggagacc aaatacaaca agctttgcca
     2221 gcgctgccgc aataagtgca agcagtcgga gagcgttacg gtggtttcat gtccacattt
     2281 tgaggcaaag ccggtacaac tggaaatccc gctaaagttt ccgcggggcc gtcccaaaaa
     2341 gctccgctcc tgaggttttt tcttgcgcat catcaccggc aagtacaagg gtcgcaatct
     2401 cttaactgtg gatggcagaa ccacccggcc caccaccgct tacaaccggg ctatgatctt
     2461 caatgtctat tccgatctgg cgggaaaaag ggtgctggac ctctttgccg gaaccggttt
     2521 ctatggcttg gaagctttgt cacgcggggc ggagtgggtt gacctggtgg agtttgccac
     2581 ccctgctgtg gctgtgatcc tcaaaaacgt ggcgttgctg ggctgcggag atgattgcca
     2641 cgtctggcgc aagcgcgcag atgcttttct gaagggcacc gataccagat gggatgtgat
     2701 ctttctggac ccaccctatg ccaaaaacct gctcaatccc tgcctggacc tgatctatca
     2761 gcgtggatta ttgctgccgg gcggagtggc tatcgccgag cacagccctc aggaaccggt
     2821 ggctgaagcc tatcaggaat tggtgctgaa ggtgaaaacc ggccgctcca gctgtttcac
     2881 gctgctcagg gctgaagaaa aataggtccg ccccaagtcc cgttcggttg tttccaagcc
     2941 gctcaggagc ttagaacttg gtatccagcc aaatctgcac ccgggtttga accctggc
//
LOCUS       DAOOWR010000209         2990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_33005_length_2990_cov_4.252811, whole genome shotgun sequence.
ACCESSION   DAOOWR010000209 DAOOWR010000000
VERSION     DAOOWR010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33005_length_2990_cov_4.252811"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLB85_05545"
     CDS             <1..842
                     /locus_tag="PLB85_05545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU72830.1"
                     /translation="VGIGVSVLLTFIAGRLLDIREESFRLILVVTGIVGFASTAVLAM
                     IRIQEPVVKRNCQRLNWKQALLEPIGGTLRLLKENKAFAAFERSFSIYGMGFIMMQPI
                     IPIYLVDKLQLTYTANFLAKGIVSQLGMLLLSPLIGKLHDRMHPFRFMAAAFGLLMVF
                     PLLFVASSLVQGEAVLPVVIVFLAYLIFGIAMAGVNITWNMGSIYFAGDSDASIYQSV
                     HVTMTGIRGLIAPVLGFVLLKVFSLTMVFVVAAGFFLAASLVSLRDYRRWKLAAQVGV
                     AQS"
     gene            1047..1352
                     /locus_tag="PLB85_05550"
     CDS             1047..1352
                     /locus_tag="PLB85_05550"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU72831.1"
                     /translation="MRRKNMTKADLVKLVSENTGIIRKDVAVVVDSLLQAIKDSLGKG
                     HHIEIRGFGTFRLKTRKPRMGRNPKTEEKVPVPARTVPTFKFSREFKNMVVDLKINE"
     gene            1493..2662
                     /locus_tag="PLB85_05555"
     CDS             1493..2662
                     /locus_tag="PLB85_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOU72832.1"
                     /translation="MNFDREDHNLELRQLIRDTLSATLEGDFSTHPDYPYFFTRAFYD
                     MFTEVEGLPLFPQEMPEVFLRWAGLEAGKGISPEEILVLDLETTGLGRGQTIAFMIGL
                     GYYEEGRYVVEQLFLPEPEAEASSFDRLIELLEKKSVLITFNGKTFDVPILESRLLYN
                     HIWLDLRSKLHIDLLHIARRLWKNKIPSCALETIEFYILGHIREKELDIEGGLIPQTY
                     FQFLISGDAELMRRVFIHNQLDILHTAALFTLICDSVGYPPALGFDHRIDYHALARLY
                     LSQGESEAARAILTDLLAQNIVSGDIACDLGLINKKEGDLEASESCFALAAALDHPTG
                     TLEYAKLLEKRREFDSALQQARKLLDWHLARPRTNARQVEELEKRVLRLRGKLGI"
BASE COUNT          680 a          890 c          772 g          648 t
ORIGIN      
        1 gcgtgggaat cggggtttcg gtattgctca cattcatcgc cggacgcctg ctcgacatcc
       61 gggaagaaag tttccgcctc atccttgtcg tgaccggaat cgtgggattt gccagcactg
      121 ccgttctggc catgatccgc atccaggaac ccgtggtgaa acgcaactgc cagcggctga
      181 actggaaaca ggccctgctg gagccgatcg gagggacgct gaggctgcta aaggaaaaca
      241 aggctttcgc cgccttcgaa cgcagctttt ccatctacgg aatgggcttc ataatgatgc
      301 agcccataat ccccatctat ctggtggaca agctgcagct cacctatacc gccaacttcc
      361 tggccaaggg catcgtttcc caactgggca tgctgctgct ctcacccctg atcggaaaac
      421 tgcacgaccg catgcaccct ttccgcttca tggcagccgc ttttggactg ctgatggtat
      481 ttccgctgct ctttgtcgcc tcatccctgg tgcagggcga agcagtgctg ccagtggtga
      541 tagtgttttt ggcctatctt atcttcggca tcgccatggc tggtgtcaac atcacctgga
      601 acatgggctc catctacttt gccggcgatt ccgacgcctc catctaccag agcgtgcacg
      661 ttaccatgac cggaattcgg ggcctgatcg ctccggtgct ggggtttgtg ctgttaaagg
      721 ttttcagcct caccatggtt ttcgtggtgg cagccggatt ctttcttgcc gcctcgctgg
      781 tgagcctgcg cgattaccgc agatggaagc tggcggcgca agtgggtgtc gcgcaatcct
      841 aatgtatttc atgccgggca aaaagcacag ttttaagcca gcgccagacc cgcctgcggg
      901 cacagcctcc gccatcttgt ctgcgctatg tgacgcggtc tccggcggtt cccggctttt
      961 ttttcttgac acaaacctaa gcttataatt tagagtatct tattaacgaa atcaatatcg
     1021 cttcggcgac gcatagctta cactatataa ggagaaaaaa catgacaaaa gcagatctgg
     1081 taaaactcgt ctctgagaac accggcatca tccgcaaaga cgttgcggtg gttgtcgatt
     1141 ccctgctgca ggctatcaaa gacagcctgg gcaagggtca ccacatcgaa atccgcggtt
     1201 ttggcacctt ccgcttgaaa acccgcaaac cccgcatggg acgcaatcct aagactgaag
     1261 agaaagtacc ggttccggcc aggaccgttc ccaccttcaa gttctcccgc gaattcaaaa
     1321 acatggtcgt tgacctcaaa atcaatgagt agagtgagcc aaaatgccct gcggtaaaaa
     1381 gagaaagaga cataaaatcg ccactcacaa gcggaaaaaa cgcctacgta aaaaccgtca
     1441 taaaaagaaa taaatcgtag ccacccttcg gggtggcttt tttggcttgg gcttgaattt
     1501 cgaccgcgaa gaccacaacc tggaactgcg ccagttgatc cgagacaccc ttagcgcgac
     1561 gctggagggg gatttttcca cgcatccgga ttatccatat ttcttcaccc gcgcgtttta
     1621 cgatatgttc accgaggtgg aagggctgcc cctctttccc caggaaatgc ctgaggtctt
     1681 tttgcgctgg gccggactgg aagcgggaaa agggatcagc ccggaggaaa tcctggttct
     1741 ggacctcgaa acaacggggc tgggacgcgg ccagaccatc gcctttatga tcggattggg
     1801 ttattatgag gaggggcgct atgtggtgga gcagctcttc ctgcccgaac cggaggctga
     1861 ggccagctct ttcgaccgcc tgatagagct tttggaaaag aaatccgtgc tgatcacctt
     1921 caacggcaaa accttcgacg tgcccatcct ggaatcccgc ctgctctaca accacatctg
     1981 gctggacctg cgttccaagc tgcacatcga cctgctccac atcgcccgga ggctctggaa
     2041 aaacaaaatc ccctcctgcg ccctggaaac catcgaattc tacatcctgg gccacatccg
     2101 ggaaaaggaa ctggacatcg agggcggcct catcccccaa acctatttcc agttcctgat
     2161 cagcggggat gccgaattga tgcgtcgcgt tttcatccat aaccagcttg acatcctcca
     2221 caccgccgct cttttcaccc tgatctgcga cagcgttggt tatccacccg ccctgggttt
     2281 tgaccaccgc atcgactacc acgccctggc ccggctctat ctcagccagg gcgaaagcga
     2341 agctgcacgc gccatcctaa ccgacctgct ggcccaaaac atcgtttccg gggacatcgc
     2401 ctgcgatctt ggtctgatca ataaaaagga gggcgatttg gaggcctcgg aaagctgttt
     2461 cgcccttgca gcagccctcg accatcccac cggaacgctg gaatacgcca agcttctgga
     2521 aaaacgccgg gagttcgaca gcgcccttca gcaagcccgg aagctgctcg actggcatct
     2581 ggcccggcct cggaccaatg ccaggcaggt ggaggaactg gagaaaaggg ttctgcgcct
     2641 gcgcggaaaa ctgggcatct gaccccaaaa gctccaaacc ggattgataa agcccggaga
     2701 gtagcagaaa tgtctgccca tcatcacttc ctgccagcca gacccttagc gccttcgttt
     2761 atctccctcc ctttcctccc gtataatcct cgcatcgaat gcgggcatca tgcgggaggt
     2821 gcgactggga aacaggaaag cgccttaacg gccagactgc atcgcatcta cacggtcaca
     2881 cgctggcgtc gagcgaggaa cgagcatcga cgccagcaac acttcctgta gtccatgctc
     2941 gcaggctcgc tggactccag gctacatacc gatgtcggaa acggtatccg
//
LOCUS       DAOOWR010000210         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_33346_length_2967_cov_2.896635, whole genome shotgun sequence.
ACCESSION   DAOOWR010000210 DAOOWR010000000
VERSION     DAOOWR010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33346_length_2967_cov_2.896635"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..674)
                     /locus_tag="PLB85_05560"
     CDS             complement(72..674)
                     /locus_tag="PLB85_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72833.1"
                     /translation="MTPNFFFKINLNKYGEIKLQTERENKAFRNAVIGFLLGALIMFA
                     GLLYLNNNLKRKVKNRKAYYTKIDSQLSEYETSGEYLSVNDVTRLAETFNDRIFWTKK
                     LEALSQEIDKQLAVRKFQFSNGVLTLNGIIEVDKRIQEGDVTFDFVQRLKANPEISND
                     FPEIKSGSKNRQIAKDTEILEFLIECYSRNSSELSRKAVQ"
     gene            complement(685..1755)
                     /gene="pilM"
                     /locus_tag="PLB85_05565"
     CDS             complement(685..1755)
                     /gene="pilM"
                     /locus_tag="PLB85_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOU72834.1"
                     /translation="MKKAKTTKIKESVGIDIGSHSVKAVHLKKLHEGFRLLNCEIRPT
                     VPIGVEYVPSDLRPDRFAPVIVEIMKAMHINPKKIQHLVTSVGGDNTSIKQIKTIFLP
                     DDELESALFFEAKKHIPISGTDMVLDFQVIDVEEKTNNMNILLAATSKELLNEHTNTL
                     VTAGLNPNIVDIDSLAVANSFALNAFVEEGVYVLLNLGAHRTNMVIWGPDSRLFARDI
                     PFGGYNFTRDIMRKRQMEWEEAENYKLEFGLMDDPGAVSEQTISVLDISEKSTEDVIV
                     EELRRSLRFYVKEAGNSDFRKLYLMGGTAKLKGLREYIEERVSVPTDIFMPFINVEMP
                     EKFADKKDPQLALAIGLAMRQE"
     gene            complement(1791..2753)
                     /locus_tag="PLB85_05570"
     CDS             complement(1791..2753)
                     /locus_tag="PLB85_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72835.1"
                     /translation="MYNNQYTVRDTVWSFFHNGTAMNTSKWVNNKLWIKGNFGTYQTW
                     GCGDTLFLLDDITLMNTAVGTDPIGNRTDVVGLVSEKSIVIKYGYRDPIDSTRVHGTS
                     GPDTGNGGIWIYAAMAALGDDGPDNSHGDGVFTFEYQHPHPSVPDVTVNNVTYTRIDL
                     HRYRFPHRPGQLWASTGPGNRNLRIDYPWYNPLWPERTPYLERGYINVYGSISQRRRG
                     FVHRSYYDYEWPSNNNWDQPIDHCGPTSSPQAVEHIDPVFGFTLTNVNFPGASGTGTG
                     YKKNYYYDNRFYRISPIDFPEVNRKDETPFAAVNWVIKRPPEYL"
     gene            complement(2769..>2967)
                     /locus_tag="PLB85_05575"
     CDS             complement(2769..>2967)
                     /locus_tag="PLB85_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72836.1"
                     /translation="RSLPWSGAAAPASSIRRRNNVVGPQYNNPNVIMYVDVDGAGYAA
                     YMGEVMEPYQQITPVYNPYPP"
BASE COUNT          625 a          749 c          823 g          770 t
ORIGIN      
        1 accggccagc atgaaaaaca gcacagagat caggaccatt cccaagatga agacagtata
       61 tttttctttc attattgcac cgccttccgg ctcagttccg aggaattgcg gctgtaacat
      121 tcgatgagga attccaaaat ctcggtgtcc ttggcaatct ggcggttttt agagccggat
      181 ttgatctcgg gaaagtcgtt gctgatctca gggttggcct tcaggcgctg gacgaaatcg
      241 aatgtaacat cgccttcctg aatgcgcttg tccacttcga taatcccgtt tagggtgagc
      301 acaccgttgc tgaactggaa cttacgcaca gccagttgct tgtcgatctc ctggctgagg
      361 gcttccagct tcttggtcca gaaaatgcgg tcgttgaagg tttcagccaa tctggtgacg
      421 tcattcaccg agaggtactc accgctggtc tcatactcgc ttagttggga atcgatcttg
      481 gtatagtagg ccttgcggtt tttaactttc cttttgaggt tgttattcag ataaaggagc
      541 ccggcgaaca tgatcagtgc acccagcaga aagccaatga cggcattgcg gaaggctttg
      601 ttttcccgct cggtctggag cttaatctcg ccgtatttgt tcaggttgat cttgaagaaa
      661 aagtttggtg tcatactcac gtacctattc ctgccgcata gccaggccta tggccagggc
      721 cagctgcgga tcctttttat ctgcgaattt ctccggcatt tccacattga tgaagggcat
      781 gaaaatgtcc gtgggaacgc tcactctttc ctcaatgtat tcgcggaggc ctttaagctt
      841 ggctgttccg cccataaggt aaagcttgcg aaagtcgctg ttgcctgctt ccttgacgta
      901 gaaacggagc gaacggcgca gttcttccac gatcacgtct tcggtggatt tttccgatat
      961 gtcaagcacg ctgatggttt gctcgctgac cgcgccggga tcgtccataa ggccgaattc
     1021 cagcttgtag ttctcggcct cctcccactc catctggcgc ttgcgcatga tgtcccgggt
     1081 gaaattatag cccccaaagg ggatgtcgcg ggcaaacagc ctggaatcag gcccccagat
     1141 caccatgttc gtacggtgcg cccccagatt cagcaacaca tacaccccct cctctacaaa
     1201 ggcgttgagg gcaaagctgt tggccactgc cagggagtcg atatccacaa tgttgggatt
     1261 caggccggct gtgaccaggg tgttggtgtg ctcgttcagc agttccttgg aggtggcggc
     1321 caggaggatg ttcatgttgt tcgttttctc ctcaacatcg atcacctgga aatccaggac
     1381 catgtccgtg ccgctgatgg gaatgtgttt cttcgcttcg aagaacaggg cggattccag
     1441 ttcgtcatca ggcagaaaga tggtcttgat ctgcttgatg ctggtgttgt cccctcccac
     1501 agaggtaacc agatgctgga tctttttggg attgatgtgc atggctttca tgatctcgac
     1561 gatcaccggg gcgaagcgat cggggcgaag atcgctgggc acgtattcca caccaatcgg
     1621 gacggttggc cggatttcgc agtttagcag cctgaagcct tcgtgcagct ttttcagatg
     1681 caccgccttg acgctatggc tgcctatgtc gattccgacc gattctttga tcttggtcgt
     1741 tttcgctttc ttcatcgttc ctcttatctc tgacgtttaa tatataagta tcacaggtat
     1801 tccggcgggc gcttgataac ccagttcacc gcggcaaaag gagtctcgtc tttgcggttg
     1861 acttccggaa aatctatcgg cgaaatccgg taaaacctgt tgtcgtaata atagttcttt
     1921 ttatagccag tgcctgttcc cgaagccccg gggaaattga cgttcgtgag ggtaaacccg
     1981 aaaacgggat cgatgtgctc caccgcttgt ggcgagctgg tagggccaca gtgatcgatg
     2041 ggctgatccc agttgttgtt cgaaggccat tcataatcgt aatagctgcg gtgcacgaat
     2101 cccctgcggc gctgggaaat ggaaccgtaa acgttgatat agcctcgttc caggtagggt
     2161 gtgcgctcag gccagagggg attgtaccag ggatagtcga tcctcaggtt gcggtttcca
     2221 ggtcccgtgg aggcccaaag ctgcccggga cggtggggga agcggtagcg atgcaggtcg
     2281 attctggtgt aggttacgtt gttcaccgta acatccggca cggagggatg cggatgctgg
     2341 tactcaaagg taaaaacccc atcgccgtgt gagttgtcgg gtccgtcatc acccaatgcg
     2401 gccatggccg cgtagatcca gatgccgcca ttaccggtgt ctggaccgga agtcccatgc
     2461 acgcgcgtgg agtcgatcgg gtcacggtac ccgtatttga tcacgatcga tttctcggag
     2521 accaagccaa ccacatcggt gcggttgcct atggggtcgg tgcccacagc cgtgttcatc
     2581 agcgtgatgt catccagcag aaagagggtg tcgccacatc cccaagtctg gtatgtacca
     2641 aagtttccct taatccagag tttgttgttc acccacttgg atgtgttcat ggctgtgccg
     2701 ttgtggaaaa aagaccagac ggtgtcccgc acggtatatt ggttattata caaaaatgtg
     2761 gcagggtttt aaggaggata ggggttgtac acaggcgtga tttgttgata gggttccata
     2821 acttcgccca tataagctgc atagccggcg ccgtccacat ccacgtacat aataacgttt
     2881 ggattattat attgagggcc cacaacgttg tttctgcggc ggatgctgga ggccggggcg
     2941 gcggcacccg accacggcaa gctgcgg
//
LOCUS       DAOOWR010000211         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_33568_length_2951_cov_3.054213, whole genome shotgun sequence.
ACCESSION   DAOOWR010000211 DAOOWR010000000
VERSION     DAOOWR010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33568_length_2951_cov_3.054213"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLB85_05580"
     CDS             complement(<1..870)
                     /locus_tag="PLB85_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOU72837.1"
                     /translation="MKKLLLLAALLTALLPVFGQWHIDEDFEGITTLPDGWTFHDDGD
                     GMTWRNINNDSHAHSGTRVAFCDNYFPNQNSDWLITPQITVAAGDSLIFWTRSWISTE
                     PLKVFVSTTGTAVGNFNTLLADISEIGTSYQEVRLSLAPWTGQAIYIGFLWECENYGI
                     LIDDVRAGQPVIIQPELDLPESITFYQGESLTMDFTPYITTTDLNTASLTCSGAVNVS
                     VSINRLSAEFSCANWNGTENITFTLHDGTSGLGDSDILSVIVLPPLSVDLAVTEVHSP
                     MEFQFLNLPFTPMV"
     gene            complement(884..2251)
                     /locus_tag="PLB85_05585"
     CDS             complement(884..2251)
                     /locus_tag="PLB85_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72838.1"
                     /translation="MIRNLQLIQRHAQLKELGSQLKLALLKELIHAPATCQQLANLFD
                     QSKQKIHYNLNQMLKEGLLQIVELPSGGGREVYYRATARTYVLDLSIGLENNGNVLNS
                     RAIINGIMERDHHIDLAGVAAKLIDQAFKLVPGERLLIVSGKFNFPLAEKLKLEAGRR
                     GIFCTLIYQDLDALKAKYEQFSLAAFKADYEELNRHLKTADVYLNLNGEARFLQLKDP
                     KKLALRNRMLERSKQIIRERGVRVAMMPGLLQDTLSEGAIEGEVQFWRALDIDYKRQC
                     CQTLAKCRELSGKDTVRLCGRNSSLKFRIEKIVAECGSFGQNEFQSPVINLPGGEILM
                     IPKAGSMNGTLASERIHVFGEEVLRPVLEIGNNEIVGYQAQKGAEHLARALESGGADG
                     RKAALICLGTNENIHLGDIDMALKHKSSGNLSVFWGDNRSLGGDVSGAYEWFMQIEEP
                     LLSFN"
BASE COUNT          620 a          802 c          793 g          736 t
ORIGIN      
        1 caccataggt gtgaagggca ggttcaggaa ttgaaactcc atcggactgt ggacttcggt
       61 cacagccaga tcgacactca gcggcggcag cacaattacg ctcagaatgt cgctgtcgcc
      121 gagcccggaa gtcccgtcgt gcagggtgaa agtgatgttt tcggtgccgt tccagttcgc
      181 gcagctaaat tcagcggaaa gcctgttaat gctcaccgag acgttcactg ctccactgca
      241 ggtgagcgaa gccgtgttca ggtctgtcgt ggtgatatag ggcgtgaagt ccatggtcag
      301 gctttctccc tgatagaacg taatgctttc cggcagatcg agttccggct gtattatcac
      361 aggttggccc gcgcgcacgt cgtcaatcag gatgccgtag ttttcgcatt cccagaggaa
      421 gccgatatag attgcctgtc ctgtccaggg cgccaggctg agccggacct cctgatagct
      481 ggtgccgatt tcgctaatat ccgccagcag ggtgttgaaa ttgccaaccg ccgtgccggt
      541 ggtggaaacg aagaccttta ggggttcggt acttatccaa ctgcgggtcc agaagatcag
      601 gctgtcgccc gctgccacag tgatttgcgg cgtgatcagc cagtcggagt tctggttggg
      661 gaaatagttg tcgcaaaaag cgacccgcgt tccgctgtgg gcgtgggagt cgttgttaat
      721 gttgcgccag gtcatgccgt ctccgtcgtc gtggaaggtc cagccatcgg gcagggtggt
      781 gatcccttca aaatcctcgt cgatgtgcca ttgtccaaaa accgggagca gcgctgtcag
      841 cagggccgca agcaataata gtttcttcat gttgacctcg tttttaattg aagcttagca
      901 gcggttcttc gatttgcatg aaccattcgt aggcgccgga aacgtctccc cccagggagc
      961 ggttgtcgcc ccaaaagacg ctcagattgc cgctgctttt atgcttgagc gccatgtcga
     1021 tgtcgcccag atggatgttt tcattggtgc cgaggcagat cagtgcggct ttgcgtccgt
     1081 ccgcgccacc gctttccaaa gcccgggcca ggtgttccgc tcccttctgg gcctggtagc
     1141 cgactatctc gttgttgccg atttccagca cgggacgcag cacctcctcg ccaaaaacgt
     1201 ggatcctttc actggccaga gttccgttca tgctgccggc tttgggaatc atcaggattt
     1261 cgccgccggg aaggtttatc accggcgact ggaattcgtt ctggccaaaa gaaccgcatt
     1321 ccgccacgat tttctcgatg cgaaacttta gcgaactatt tctgccgcac agccttacag
     1381 tgtcttttcc gctcagctcg cggcacttgg ccagggtctg gcaacattgc cttttataat
     1441 cgatgtccag agcccgccag aattgcactt cgccctcgat ggcaccctcg gaaagtgtgt
     1501 cctgcaaaag gccgggcatc atggcaacgc ggacaccgcg ttcgcggatt atctgtttgg
     1561 accgctccag catgcggttg cgcagagcca gctttttcgg gtctttcagt tgcaggaaac
     1621 gcgcttcgcc attgaggttc agatacacgt cggctgtctt cagatgccgg ttcagttctt
     1681 cgtaatcggc cttgaaagcg gctagggaaa actgctcgta tttggctttc agcgcgtcca
     1741 gatcctgata gatcagggtg cagaaaatcc cgcgccgccc ggcttccagc ttcagctttt
     1801 ccgccagggg gaagttaaat ttgccgctca cgatcagcaa acgctcaccg ggaaccagct
     1861 tgaaggcctg gtcgatcagt ttggccgcta ccccggccag gtcgatgtgg tggtcgcgct
     1921 ccatgatgcc gttgatgatg gctcggctgt tcagaacgtt gccgttgttt tccaggccga
     1981 tggaaagatc cagcacatag gttctggccg tggcccggta ataaacctca cggccgccgc
     2041 cggaagggag ctcaactatt tgcagcaggc cctctttgag catctggttg aggttgtagt
     2101 ggattttctg tttgctttgg tcaaaaagat ttgccagttg ctggcaggtc gcgggggcgt
     2161 ggataagctc tttgagcaga gccagcttca actggctgcc cagttccttc aattgcgcgt
     2221 ggcgctggat cagttgcagg tttcggatca tctgaaacac cttgttgctt gcatgatttg
     2281 catcctgaaa ccagattgca caggctgtgt tattagtcaa gaactttttg accatttttt
     2341 cctaagaacc ctttagcctt ccttaaatca atacggaatc aagggaggaa agatgcccaa
     2401 tttcttcaat ggtgaaaaaa ctagagcgcc tttccctttc aagttctctt tgaaccggga
     2461 agctgccctc aatcatccaa aaagcaacct ggagagctta aatgttagtc caccacaata
     2521 taatgccagt caggccctga gcgctcgcgt ccatgcctct cccccgcctc ccgtaatata
     2581 cccgcatcaa atgcggccat tatgcgggag gcatgtccgg gaactgggaa agggccttaa
     2641 cggcctgtcg gcaccgcttc tttcctgcta cacgccagcg taaagccagg aacgaacacc
     2701 gacggcggca atgaataata tgcccagcac acttattagt tcattgccgc agattatcct
     2761 gtcgtcaata cttactacgt tcgctgtact ccaagccgtc tctgggcttt acccatccga
     2821 tttgaaagaa agcataatca ttccgaccag aacttggatg tgtttgattg ttttgccaaa
     2881 cgcaacagac ccatagttgt taacgaaggc gaaattgttc aggtggtgat tcccatgcat
     2941 tctgtaaact c
//
LOCUS       DAOOWR010000212         2946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_33638_length_2946_cov_3.755448, whole genome shotgun sequence.
ACCESSION   DAOOWR010000212 DAOOWR010000000
VERSION     DAOOWR010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33638_length_2946_cov_3.755448"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLB85_05590"
     CDS             <1..483
                     /locus_tag="PLB85_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU72839.1"
                     /translation="GTAVVIIKKSLLEKAIPGAPSMLDYNLMAKNGSMFNTPATFTIY
                     MIGLVLKWIEDFGGLAKIEQNNIVKAKYIYDAIDASEGYYKGTVEKEDRSLMNITFRL
                     ASEELEAMFIAEAKKNGMIGLKGHRNVGGCRASTYNSLPLPAAHALGEFMTEFAKKHG
                     "
     gene            562..1197
                     /locus_tag="PLB85_05595"
     CDS             562..1197
                     /locus_tag="PLB85_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU72840.1"
                     /translation="MSIMNLLLRGGILMYVLVVVSIAVLAIVLEKYRQVSRLRRANEK
                     LRYYLSQQDKLDNIRAVLRIHGPNSPLGMMLDKLYNSQTDDFRLIENSMESTANLELR
                     KLEKGMGWLATLSAIAPLIGFLGTVMGMVSVFMNIQGQGQNSVDINTLAGGIWEALLT
                     TVGGLVVGIPAIIFHNDLVEHMTNIAKDMQNQAIEHEIRYKKALDKEAQRP"
     gene            1194..1598
                     /locus_tag="PLB85_05600"
     CDS             1194..1598
                     /locus_tag="PLB85_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU72841.1"
                     /translation="MKLEISRQKISSTVLISMTDVIFLLLIFLLIASNFAAQTGLPFK
                     LPGSVSQERQTRQVLHIQYLNEKNIIFLDKSYNLDTLGEALKSEFNNPEQVVRLSAEK
                     TTPLQSVINLMDVVRGAGFERVFVATEPVTGP"
     gene            1595..2302
                     /locus_tag="PLB85_05605"
     CDS             1595..2302
                     /locus_tag="PLB85_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72842.1"
                     /translation="MISFRAGNHRSLALSLLIHALLLVMLTMYIIKPMQAPRWYEFEL
                     APPELHPDAVVTTPGPGASQPLIQTETGKPTTTKPNQTKPQTSQPEKPAVASEQPQAS
                     ARSELLETPAITTTIPAKPTALPTNPLNPLRGIPTNRPGSTAPGGSVNYSLGGGGVRF
                     QLPDDYKHNLGAAGRVVITFRLDQNARPIMSSVESSEQSGPRYFDAAKKMLQDGKFSY
                     TGSPNPGREYSLTINFL"
     gene            2353..>2946
                     /gene="folP"
                     /locus_tag="PLB85_05610"
     CDS             2353..>2946
                     /gene="folP"
                     /locus_tag="PLB85_05610"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU72843.1"
                     /translation="MGILNVTTDSFSDGGRFIDPANALAHARKLVDDGADILDIGGES
                     TRPGSLPVSPSEELRKVLPVLEKIRENWPDLPISVDTRNSELAEAAIELGATYINDIS
                     ALNHDPRMAGLVAANPQVKLVLMHMRGQPETMQTDPVYTDLLGEIDDFFRERIEFAVS
                     QGIDRANLVLDPGIGFGKTAEHNFKLLGHLADFHKYGL"
BASE COUNT          753 a          857 c          741 g          595 t
ORIGIN      
        1 ggaacagcag tcgtgatcat caaaaagagc ctgctggaaa aagcaatccc tggcgccccc
       61 tccatgctgg attacaatct gatggccaaa aacggctcca tgttcaacac acccgccacc
      121 ttcaccatct atatgatcgg cctggtgctg aagtggatcg aagacttcgg cggactcgct
      181 aagatcgagc agaacaacat cgtcaaggcc aaatacatct acgacgccat cgacgcctcc
      241 gaaggctatt acaaaggcac cgtggaaaag gaagaccgtt ccctgatgaa catcaccttc
      301 cgtctcgcga gcgaggagct ggaagccatg ttcatcgcgg aagccaaaaa gaacggcatg
      361 atcgggctca agggacaccg caacgttggc ggctgccgcg ccagcaccta caattccctg
      421 cccctgcccg ccgcccacgc tttgggtgaa ttcatgaccg aattcgccaa gaagcacggt
      481 tgataataaa gacaacacat aatgtatgca aggcgggaga aaacagcctc ccgccttgtt
      541 taaagcagat ccgggagtga tatgtccata atgaacctct tgctgcgcgg cggcatactg
      601 atgtatgtcc tggtggtggt gtccatcgcc gtgctggcca tcgttctgga aaagtaccgc
      661 caggtttccc gcctccgcag agccaacgaa aagctccgct attacctgtc ccagcaggac
      721 aagcttgaca atatccgtgc cgttttgcgc atccatgggc caaacagtcc cctgggcatg
      781 atgctggaca agctttacaa ctcacagacg gatgatttca ggctgataga aaacagcatg
      841 gaatccaccg ccaatcttga gctgcgcaag ctggaaaaag gcatgggatg gttggctacc
      901 ctttctgcga tcgcgcccct gatcggcttt ctgggcactg tgatgggcat ggtcagcgtg
      961 ttcatgaaca tccagggcca gggccagaac agcgtggaca tcaacaccct tgccggcggt
     1021 atctgggaag cgctgctcac cacggttggc ggtctggtgg tgggcattcc ggccatcatt
     1081 ttccacaacg acttggtgga gcacatgacc aacatcgcca aggatatgca aaaccaggcc
     1141 atcgaacatg agatcaggta taaaaaagcc ctggacaagg aagcgcagcg gccatgaaac
     1201 ttgaaatctc gcgccagaaa atcagcagca ccgtgcttat ctcaatgacc gacgttatct
     1261 tcctgctgct gatcttcctg ctcatcgcct ccaatttcgc cgctcagacg ggactgcctt
     1321 tcaagctgcc gggatcggtt tcccaggaac gccagacgcg ccaggttctg cacatccagt
     1381 atttgaatga gaaaaacatc atctttctgg acaaatcata caacctggac acgctgggag
     1441 aagcgctgaa aagcgaattc aacaatccag aacaggttgt gcgcctgtcc gcggagaaaa
     1501 caacgcccct gcagagcgtc atcaacctga tggacgtggt gcggggagct ggtttcgagc
     1561 gggtgttcgt tgccacggaa cccgtgactg ggccatgatc tcatttcgcg caggcaacca
     1621 ccgttctctg gcgttatcac tgctgataca cgccctgctt ctggtgatgc taaccatgta
     1681 tattatcaag cccatgcaag cgccgcgctg gtatgagttc gagctggcgc cgccggaatt
     1741 gcatcctgac gcggtggtta ccactccagg ccccggagcg tcccaacccc tgatccagac
     1801 tgaaaccggg aagcccacta ccaccaaacc aaatcagacc aaaccgcaaa ccagccagcc
     1861 tgaaaaaccg gccgtggcct ctgagcaacc ccaagcttcc gcgcgcagcg aacttcttga
     1921 aacacctgcc attacaacaa ctatcccagc caaaccgacc gcccttccca ctaatccact
     1981 aaatcccctg cgcggaatac ccacaaaccg ccctggcagc acagctcccg ggggtagtgt
     2041 aaactattcc ctcggcggag gaggggtgcg ttttcagttg ccagacgact ataaacacaa
     2101 tttgggcgcc gcaggcaggg tcgttatcac tttcaggctt gaccaaaatg cccgtcccat
     2161 catgagtagc gtggaaagtt ctgagcaaag cggccccaga tacttcgatg ccgccaaaaa
     2221 gatgctgcag gatggcaaat tcagttatac aggctcacca aatcccggca gggaatatag
     2281 cctcacaatc aattttctct aattatgccc caaaataccc tacttcccaa aaacttcaaa
     2341 gtcccacaat tgatgggcat cctgaacgtt accaccgact cgttttccga tggcggaagg
     2401 tttatagacc ccgcaaatgc tttggcccat gccaggaaac tggttgacga tggagccgac
     2461 attctggata tcggaggtga atcaacccgc cccggatcgc ttcctgtgtc tccatcagag
     2521 gaactgcgca aagtattgcc ggtgcttgaa aagataaggg aaaactggcc ggacctgccc
     2581 atttcggtgg acacccgcaa cagcgagctg gctgaagccg ccatagaact gggggctact
     2641 tacatcaacg atatttcagc cctgaatcac gatccgcgca tggccgggct tgtggctgcc
     2701 aatccacagg ttaagctcgt tctcatgcac atgcggggac agcctgaaac gatgcagacc
     2761 gatccggttt acactgattt gctgggcgaa atcgacgatt tcttcaggga gcggatagaa
     2821 tttgctgtct cacagggaat agaccgcgcc aacctcgttc tcgatccagg tatcggtttc
     2881 ggtaaaacgg cagagcacaa tttcaagttg ctgggacatc tggcggactt ccacaaatac
     2941 ggattg
//
LOCUS       DAOOWR010000213         2891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_34483_length_2891_cov_3.566291, whole genome shotgun sequence.
ACCESSION   DAOOWR010000213 DAOOWR010000000
VERSION     DAOOWR010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34483_length_2891_cov_3.566291"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLB85_05615"
     CDS             complement(<1..480)
                     /locus_tag="PLB85_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72844.1"
                     /translation="MPKTILIILALLVAALPLTALRSIMDSYQAEARGDYNDALAIAR
                     ELLDAAPNDAFYQVRVAWLLYLQGNYGDAASAYEASIRLQDSLDAQLGRLNSLLALGR
                     YTEALQHSRAQIVLHPENTVLLGKGAYAAYMLKDYRTAAEFFGRIAVLYPWDMENREY
                     "
     gene            complement(473..2680)
                     /locus_tag="PLB85_05620"
     CDS             complement(473..2680)
                     /locus_tag="PLB85_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOU72845.1"
                     /translation="MSKIAAAALPFLKAIPHSYATVMFSDNLWLGLALLALTLASPIV
                     GMAGLLGLLVALAATRLTGFEDWHSGSGVLAFNSLLISLTIGYYYPLSGVREQPLPFV
                     CLIVIASLATLLLYVVLNYTTMNALKLPSMSLAFSIMGTLIWYYLVRSGNFNGEGFLK
                     PLLFNLKLDLPWFWRNYFLSMGSIMFVPDIAVGIGVALILLMITRIGSLLSLLGWAIC
                     WWLLGFASIGTTYGMFFPGFNLILISISVGSVYLIPGKSSYLMAALATIFGFGISFAL
                     SGKYFYADYMPGRGAYLMVPMFAFPFNLVVLTTIFALRQRLAHRSPVLNEVGILHPEK
                     ALDAYMSRYKRFSSTGIPQLLLPVTGEWTITQGHNGAHTHQKEWANAWDFEIEDVNGK
                     IYSEDPTSLKDHYAFGKPVHAAAAGYVAKVVNGIADNPIGITNTQDNWGNYVSIYHMA
                     GYYTLYAHLKEGSIKFSEGDYVKQGEKIGQVGNSGRSPVPHLHFQAQSGAEAGTKSIY
                     CHLLNYKVHGADGAQRFIGSGIPKERERISPLIPEKELANLLQLGYHQKQSFLVQNGG
                     TEHTENWEVELDLPGIHRLNSDSGCSLEFSIYNGIYNSLNLSSRRLCALSAFALGASR
                     IPWAENAELAWEDEPSLSVVMNSFWKNLTLFLIPFFQPIRARTVSKLAPADKDLVQTS
                     ETTLKVLGITVRSHQSRIILSRRDGLKSIELAHNGKTLLTATKIKTQEENELA"
     gene            complement(2677..>2891)
                     /locus_tag="PLB85_05625"
     CDS             complement(2677..>2891)
                     /locus_tag="PLB85_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU72846.1"
                     /translation="AVPFPDLYTGEQVLVHPIGAYNNTQWMQFIEYRPKIVLISETGK
                     PELIRDKEELSDIIEREYIPEHLKQI"
BASE COUNT          628 a          746 c          875 g          642 t
ORIGIN      
        1 gtattccctg ttttccatat cccaaggata gagcacagcg atgcggccga agaattcagc
       61 cgcggtgcgg taatccttta gcatgtaagc cgcgtaagca ccttttccca gcagaacggt
      121 gttttccgga tgcagaacga tttgagcgcg gctgtgttga agggcttcgg tgtagcggcc
      181 caaggccagc agggaattca gccggcctag ttgggcgtct aggctgtcct gcaggcggat
      241 ggatgcttca taagcgctgg cggcatcgcc gtagtttccc tgcagatata gcagccaggc
      301 aacgcgcacc tgatagaaag cgtcatttgg agctgcatcc agcagttcgc gcgcaatggc
      361 cagggcgtcg ttataatcgc cgcgcgcctc ggcctggtaa gaatccatga tgctccgcaa
      421 ggctgtaagt ggcaaggccg caaccagcag ggcaaggatg atgaggatgg ttttaggcaa
      481 gttcgttttc ctcctgggtt ttgattttgg tggcggtcaa cagggttttg ccgttatgcg
      541 ccagttcgat tgatttgagg ccgtcccggc gggagaggat gatccgcgat tggtggcttc
      601 ggaccgtgat ccccagtact ttcagggtgg tttcgctggt ctggacaagg tctttgtccg
      661 ctggtgccag tttggaaacc gttcgcgcgc ggatcggttg gaaaaagggt atcagaaaga
      721 gagtgaggtt tttccagaag gaattcatca ccacggagag gctgggttcg tcctcccagg
      781 ccagttctgc gttttccgcc caggggatgc gtgaagcccc aagggcaaag gcgctcagcg
      841 cgcagaggcg gcggctggaa aggttgaggg aattgtagat gccattgtag atggaaaact
      901 ccaggctgca gccgctgtcg gaattaagac ggtggatgcc cggcaggtcg agctcaacct
      961 cccagttttc ggtgtgttcc gttccgccgt tttgcaccag gaagctctgt ttttgatggt
     1021 atccaagctg gagaaggttc gccagttcct tttccgggat caggggggaa atcctctccc
     1081 tttctttggg gatgccgctg cctatgaatc tttgggcacc gtccgctccg tggaccttgt
     1141 agttcagaag atggcagtaa atgcttttgg tgccggcttc agcgccggat tgggcctgaa
     1201 aatgcagatg gggcacaggt gagcggccgg aatttcccac ctggccaatc ttttcgccct
     1261 gcttcacgta gtcgccttcg ctgaacttga tcgagccttc tttcagatgg gcgtaaagcg
     1321 tatagtaacc agccatgtgg tagatgctta cgtagtttcc ccagttgtcc tgggtgttgg
     1381 tgatgccgat tgggttgtcc gcgatgccgt tcacaacctt ggccacatag ccagccgcgg
     1441 cagcatggac aggttttccg aaggcgtaat ggtctttgag gctggtggga tcttctgaat
     1501 atatttttcc gttcacatcc tcgatctcaa agtcccaggc gttagcccat tccttttggt
     1561 gggtgtgagc gccgttgtgg ccctgggtta ttgtccattc acctgtcaca ggcaggagaa
     1621 gctgcgggat gccggtgctg gaaaagcgct tgtagcggct catataggcg tccagtgcct
     1681 tttccggatg caggataccc acctcgttga gcacggggga acggtgtgcc aacctctgcc
     1741 gcagagcgaa gatggtggtg agcaccacga ggttgaaggg aaaggcgaac atcggcacca
     1801 tcagataggc tccgcgcccg ggcatatagt cggcgtagaa atacttcccg gacagcgcga
     1861 aagagatccc gaacccgaaa atggtggcca gcgccgccat cagataggag gattttcccg
     1921 ggataagata gacagagccc acgctgatgc tgatcaggat gaggttgaag ccgggaaaga
     1981 acatcccgta agtggtgccg atgctggcga aacccaacaa ccaccagcag atagcccagc
     2041 ccagaagcga aagcagagag ccgatccggg tgatcatcag caggatcagc gcaacgccga
     2101 tccccacggc gatatccggc acgaacatga tgctgcccat tgagaggaag taattcctcc
     2161 aaaaccacgg cagatcaagc ttcaggttga agagcagcgg ctttaggaag ccttcgccgt
     2221 tgaaattgcc gctacgcacc agatagtacc agatcagggt gcccatgatg gaaaaagcaa
     2281 ggctcattga gggcagcttc aaagcgttca tggttgtgta attaagcacc acatagagca
     2341 gtagtgtggc cagcgaagcg atcacaatca ggcagacgaa gggcagaggc tgctcccgaa
     2401 ccccgctcaa cggataatag tagccgatgg ttagtgagat cagcaggctg ttgaaggcca
     2461 gaaccccact gccgctgtgc cagtcctcaa aacccgtcag ccgcgttgcc gccagcgcca
     2521 ccagtagccc cagcaaaccg gccataccca cgatcggtga cgccagggta agggccagca
     2581 acgccagccc cagccacaga ttgtccgaaa acatgactgt ggcatagctg tgtggtatcg
     2641 ctttcagaaa gggcagggca gccgcggcga tcttgctcat atctgtttca ggtgttcggg
     2701 gatatattca cgctcgatga tgtcgcttag ttcctctttg tcgcggatta gctccggttt
     2761 gcccgtttcg ctgatcagca caattttggg ccggtattcg atgaactgca tccactgggt
     2821 gttgttgtag gcgccaatgg gatgcaccag cacctgttct ccggtataga ggtcagggaa
     2881 aggtacggcc t
//
LOCUS       DAOOWR010000214         2857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_35031_length_2857_cov_3.613847, whole genome shotgun sequence.
ACCESSION   DAOOWR010000214 DAOOWR010000000
VERSION     DAOOWR010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35031_length_2857_cov_3.613847"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLB85_05630"
     CDS             <1..214
                     /locus_tag="PLB85_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72847.1"
                     /translation="LITALGGVLGIILGFSILKAVGSYLDLQVIASVQMIWVALLVSV
                     GVGLIFGISPAMRASKLDPVEALRED"
     gene            217..1293
                     /locus_tag="PLB85_05635"
     CDS             217..1293
                     /locus_tag="PLB85_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOU72848.1"
                     /translation="MNDNGKKGFFTNLWALIVTYVKAVWIDLVKLRSYLTEPAKRRQV
                     LSDLWDFLKRWFRLIIRERVMREAGSLTYITILGFVPFVVFLLLIVPDLPFLNLQEKV
                     FNLISNNLMPTSALAVNNVIEGMLEGRAGFNIINFVVMIISSYSLFRTIRDSFDRILK
                     MEYHPKQDLLSQVIKFLGTIILGVFILVVLISSSSLPLISRLLRMPMLRWLTYLVPFV
                     LQFLLLVFLYTLMPSIKVQRISLVRGAFWTALIWMVVKSGFDTYILRMTSYQKFYGTL
                     SALPIFLLWIYVNWLIVLSGIVMVAVFENKKRGEEPPNGSKEMIRLTLEIYGDGKMRR
                     NLDRYISRNELKEILDPPEEEEEG"
     gene            1290..2834
                     /gene="purH"
                     /locus_tag="PLB85_05640"
     CDS             1290..2834
                     /gene="purH"
                     /locus_tag="PLB85_05640"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU72849.1"
                     /translation="MKKYALISVSDKSGAETLASGLAKLGYGILSSTGTANHLRNRAL
                     EVVEVEDVTGFPEILDGRVKTLHPHIHAGILADRSNPEHMATLDRLGIQRIDLVAVNL
                     YPFRETLAETDSTRQQIIENIDIGGPSLLRAAAKNHAGVIVLCDPADYSGVLRELQAG
                     GELPLEKCIHLARKAFAHVAAYDAAIANWLRCQKHADSEPELPDQLEVSMPRAAHLRY
                     GENPHQRAALYASGQNGLSLLHGLELSFNNQMDIDATLRALRLFEAPTVVITKHCNPC
                     GIGCAERLQDAYKKALAADTESPFGGIVGLNRALDLETARLINQIFTEIIVAPDYENG
                     VLEFLRKKKNRRLIRWDEEVFNPGQNPWELKQLLQGWLLQERDLVNEDPSTWRCVTRR
                     EPSTEEIQALGFAWKVAALLRSNAIALTGNDRVFGLGAGQTSRIDALQLAVMKARKFG
                     HSLSGAVCASDGFFPFRDSIDALHDAGVRAVIQPGGSKGDLEVIAACDELGLAMVFTG
                     CRHFRH"
BASE COUNT          631 a          776 c          805 g          645 t
ORIGIN      
        1 cctgatcacc gcgctgggcg gggtgctggg cataattctt ggcttttcca tactcaaagc
       61 cgtgggttcc tatctggacc tgcaggtgat cgcctcggtg cagatgatct gggtggcgct
      121 gctcgtttcc gtgggggtgg gacttatttt tggcatttcg ccggcgatgc gggccagcaa
      181 acttgacccc gttgaagccc tgagggagga ttgaacatga atgacaatgg caaaaaaggt
      241 tttttcacca atctctgggc gttgatagtg acttacgtca aagcagtatg gatcgatctg
      301 gtgaaactca gaagctatct gaccgagcct gccaaacgcc ggcaggtgct ttcggacctc
      361 tgggacttcc tgaaacgctg gttccgcctt atcatcaggg aacgcgtgat gcgcgaggcc
      421 ggatccctaa cctacatcac cattctgggc tttgtgcctt tcgtggtgtt cctgctgctg
      481 atcgtgccgg acctgccctt cctgaacctg caggaaaagg tgtttaacct catatcgaac
      541 aacctgatgc ccacatccgc gctggctgtg aacaacgtga tcgaaggcat gctggaaggc
      601 cgtgccggct tcaacattat caacttcgtc gtgatgatca tatcatccta ttcgctgttc
      661 agaaccatcc gcgattcctt tgaccgtatc ctgaagatgg agtatcaccc caaacaggac
      721 ctcctcagcc aggtcatcaa gtttctgggt accatcatcc tgggtgtgtt catcctggtg
      781 gtgctgatct caagttcttc gctgccgctg atatcgcggc tgctcagaat gccaatgctg
      841 cgctggctca cctatttggt gccgttcgtg ctgcaattcc tgctgctggt gtttctttat
      901 acgttgatgc cgtcgatcaa ggtgcagcgg atttcactgg tgcgcggggc gttttggacc
      961 gccctgatct ggatggtggt gaaaagcgga tttgacacct atatcctgcg aatgaccagc
     1021 tatcagaagt tttacggcac cctctcagcc ctgcccatct tcctcctctg gatctatgtg
     1081 aactggctga tcgtgcttag cggaatcgtg atggtggcgg tgtttgagaa taaaaaacgt
     1141 ggcgaagagc ctcccaacgg gtccaaagaa atgatacgcc tcaccctgga gatttacggt
     1201 gacggcaaga tgaggcgaaa tctggacaga tacatcagcc ggaacgaact caaggagatt
     1261 ctggaccctc cggaggaaga ggaggaaggg tgaaaaaata cgcattgatc tcggtttctg
     1321 ataaatccgg agcggagacc ctggcgagcg gactggcaaa gctgggctat ggcatcctgt
     1381 ccagtaccgg cacggccaat catctgcgga acagagcatt ggaagttgta gaagtggagg
     1441 atgttacggg atttccggaa atcctggatg ggcgcgtgaa gactttgcat cctcacattc
     1501 acgcaggcat cctggccgac cgctccaatc cagagcacat ggcaaccctg gaccggcttg
     1561 gcatccagcg gatcgacctg gtggccgtga atctctatcc ctttagagaa accctggctg
     1621 agaccgactc cacgcggcag caaatcatcg agaatatcga cattggcggc ccctcgctat
     1681 tgcgggcggc ggccaaaaac catgccggag ttatagtcct ctgtgatccg gcagattaca
     1741 gcggcgtatt aagagagctt caagctggag gcgaactgcc gctagagaag tgcatccacc
     1801 tggcgcgcaa ggctttcgca cacgttgcgg cttacgatgc tgccatcgcg aactggctac
     1861 gctgccagaa gcatgctgat tcggaaccgg aactgccaga ccaactggaa gttagtatgc
     1921 cccgagccgc acatttgcgc tatggggaaa acccacatca aagagcagca ttatacgcca
     1981 gtggccaaaa cgggctcagc ttgctgcacg gcttggaact ttcctttaac aaccaaatgg
     2041 acatcgatgc cacgctgagg gcgttgaggc ttttcgaagc gcccacagtg gtaataacca
     2101 aacactgcaa tccctgcggc attggctgcg ctgagcggct gcaggacgct tacaaaaaag
     2161 cgctggcggc cgacacggaa tcccccttcg gtgggattgt tggcctcaat cgcgctctgg
     2221 accttgaaac cgcgaggctg atcaaccaga tattcaccga gatcatcgtc gccccggatt
     2281 atgagaacgg ggtgctggaa tttctgcgca aaaagaagaa ccgacgcctc atccgctggg
     2341 atgaggaagt tttcaacccc gggcaaaatc cctgggaact gaaacagctg ctgcagggct
     2401 ggctgctgca agaacgcgat ctcgtgaacg aagatccctc cacttggcgc tgcgtgaccc
     2461 ggcgcgaacc ctcaactgaa gagatacagg ctcttggttt tgcctggaaa gtggctgcgc
     2521 tactgcgttc caacgccatc gccctcaccg gaaatgaccg cgttttcggc ttgggcgcgg
     2581 gacagaccag ccgaatcgac gcactgcaac tggcggtgat gaaggcccga aagtttggcc
     2641 atagccttag cggtgctgtg tgcgcctccg acggcttttt ccctttccgc gattccatcg
     2701 acgctttgca tgatgctggc gttcgcgctg taatccagcc gggcggctca aaaggcgacc
     2761 tggaggtgat agcggcctgc gacgaactgg gcttggccat ggttttcaca ggttgccgcc
     2821 actttcggca ctgatatttg atttgacaga ttacgag
//
LOCUS       DAOOWR010000215         2846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_35195_length_2846_cov_3.360444, whole genome shotgun sequence.
ACCESSION   DAOOWR010000215 DAOOWR010000000
VERSION     DAOOWR010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35195_length_2846_cov_3.360444"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1970
                     /locus_tag="PLB85_05645"
     CDS             <1..1970
                     /locus_tag="PLB85_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU72850.1"
                     /translation="LLALMPAIHASESTLTIEGGVGIPLDDIEGTNKMKGAWALSWDA
                     WMIKDWLGVGISPWFANVEVEGDDPNESYFSSLEGINLYLKLRPTKAGALNFQEKALV
                     RRISPFVALGGGWSTHGSRDENGVGSNPSVPAGFRGHFTLPHAAAGISFLSKWNTTLD
                     LGVKYDLFTKDNIDLTDNAGNHFENDALLTPYVGLGIHFGTPKAVPELIYDGKLDAFS
                     TVVGTPSAAQSYKLKGKNLKGDIDLVAPEGFVLSIDGGKVWAKSVRVPADFDGDIWVR
                     LTGEKAGTYDGSIGHQSKGTGGLSWPVNGVVREPLLPTLSLVSSGLGTFSTELGTPSA
                     PQSYKIKAKDLTGDIVINAPAGFEFSLDGGKTYASSMRVPAGFDKDILVRLTGSNPGD
                     FSGNISHTSPGVPTLNVPVTGTVTAPEVPSIILQTGKLNTFSTEVGTPSAAQSYKIKG
                     NNLTGDIMIDAPKGFEISIDGGKTWKTSVNVPAEFDKDILVRLTGAQTGKFEGNISHA
                     SPGAGTFNLPVNGTVKEAVKPEEPEQGNPILDQLEQDLLKHTVHFDTNLYTIRDGDKP
                     FLDRVAEALKKLPEIMIEIQGHTDSTGSDKINEPLSYNRAKVVKDYLVARGVDAEQIE
                     SKGYGSKVPVASNETADGKQENRRAQMVIIK"
     gene            2618..2692
                     /locus_tag="PLB85_05650"
     tRNA            2618..2692
                     /locus_tag="PLB85_05650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2650..2652,aa:Thr,seq:tgt)
     gene            2701..2786
                     /locus_tag="PLB85_05655"
     tRNA            2701..2786
                     /locus_tag="PLB85_05655"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2735..2737,aa:Tyr,seq:gta)
BASE COUNT          718 a          798 c          742 g          588 t
ORIGIN      
        1 tgctgctggc tctaatgccg gcgatccatg cttctgaaag caccctcaca atagaagggg
       61 gagttggcat tccattagat gacatcgagg gaaccaacaa aatgaaaggc gcctgggcgc
      121 tcagttggga tgcctggatg atcaaagact ggctgggcgt aggtatcagc ccatggttcg
      181 ccaatgtcga agttgagggt gacgatccca acgaatccta tttttcatct ctggaaggaa
      241 tcaatctata tctgaagctg cgccccacca aggcaggcgc gcttaacttt caggaaaagg
      301 ccctcgtaag gcgcatttct ccgttcgtgg ccctgggcgg aggctggtcc acccacggca
      361 gcagagacga aaatggcgtt ggcagcaatc ccagcgtccc ggcaggcttc aggggccatt
      421 tcaccctgcc ccacgccgca gccggcattt ccttcctctc caagtggaat accactctgg
      481 acctgggagt caaatacgac cttttcacta aggacaacat agaccttacc gataatgcgg
      541 gcaaccattt tgaaaatgac gccctgctca cgccctacgt tggcctcggc atccatttcg
      601 gcaccccgaa agccgttccg gagctgatct atgacggcaa gctggacgca ttcagcaccg
      661 tggtgggaac tccttccgcc gcccaatcct acaagctgaa aggcaaaaac ctcaaaggcg
      721 acattgatct ggtagctccc gaaggtttcg ttctttccat cgatggcggc aaggtttggg
      781 ccaaatcggt ccgcgtgccg gctgacttcg acggtgatat ttgggtgcgc ctgaccggtg
      841 aaaaagccgg gacttacgat ggcagcatcg gccaccagag caaaggcacg ggcggtctgt
      901 cctggccagt gaacggcgtg gttcgcgaac ccctgttgcc caccctgtcg ctggtttcat
      961 ccggcctggg cactttctcc accgagctgg gaactccctc cgcgccccag agctacaaga
     1021 tcaaggccaa agacctcaca ggtgacatcg ttatcaacgc ccccgcgggc tttgagtttt
     1081 ccctcgacgg cggcaagacc tacgccagtt caatgcgcgt tccggctggc ttcgacaagg
     1141 atatcctggt tcgtctgacc ggttccaatc ctggcgattt cagcggaaac atcagccaca
     1201 ccagccctgg cgtgccaacc ttaaacgtcc cggtgacggg caccgtgacc gcgccggaag
     1261 ttccctccat catcttgcag acaggcaagt taaacacgtt ttccaccgaa gtgggaactc
     1321 cctccgctgc ccaaagctac aagataaagg gcaacaacct cactggcgac atcatgatcg
     1381 acgcgccgaa gggatttgag atctcgatcg acggcggcaa gacctggaag acttctgtca
     1441 acgttccggc agaatttgac aaagatatcc tagttcgcct caccggagcc caaaccggta
     1501 aatttgaagg taatatctct cacgccagcc ctggcgccgg gactttcaac ctccctgtga
     1561 acggaacggt caaagaagcc gtcaaaccgg aagaaccaga acagggcaac cccatcctcg
     1621 atcaattgga gcaggacctg ctgaaacaca ctgtgcattt tgacaccaac ctctacacta
     1681 tccgcgacgg cgacaaaccc ttcctggacc gcgtggctga agccctcaaa aagctgcccg
     1741 agatcatgat cgaaatccag gggcacaccg actccacagg tagcgacaaa atcaacgaac
     1801 cgctttccta caaccgggcc aaggtggtga aagactacct cgtggccaga ggcgtagacg
     1861 ccgaacagat cgaaagcaag ggctacggct ccaaggttcc cgtcgcttcg aatgaaacgg
     1921 cggatggcaa acaagagaac cgccgcgccc agatggtgat cattaagtaa cgaacacttc
     1981 cacaataata aatcccaatc gggacccgtt tcacgcgggt cccttttttg gatatcttca
     2041 aaaccgaatg ggtaaagcca ggaggcaaaa gaaatgctga cccatcctaa tgtcctgtca
     2101 gccaggccct gagcgctcgt atccttccct ctcccatgcc tcccgcaaga tgctcgcatc
     2161 aaatgcgggt attatacggg aggcgcatcc gtaacatagt atagggcctt aatggccaga
     2221 ccgcatcgat ccgcttgtta gcaaggacct cgggctggga tgatggcagc gcaatccgct
     2281 ctatctgaat ataggctatc cagataagtg attcctgtca attccgctca acataccact
     2341 atcaaaaggg atttgcccac caggtgaaag caagggatgt tgggcggatt cgcgcagctt
     2401 gagaaattgg catgggtgta tcttcggtaa aagcttcctt tcattttctc agcgggcagg
     2461 ggagaggaat ttcggcaaag cagaaaaagg tcttcttgac aaaaaatggg gtggcaaatt
     2521 gtttgaactg aaccgggatt ttcggttaca acatagagtg cagcaagaaa agtgattgac
     2581 aaaaaacagg tgctgaaatt gcatggaaaa tcttgatgcc gatgtagctc aatggcagag
     2641 caattgattt gtaatcaata ggttgggggt tcaactccct tcatcggctc cagatatttt
     2701 ggacaggtgg tcgagtggtt aatggcactg ggctgtaaac ccagcgacga aagtctacgg
     2761 aggttcgaat ccttccctgt ccaccattta agcgggaata gctcagttgg ctagagcatc
     2821 agccttccaa gctgagggtc gcgggt
//
LOCUS       DAOOWR010000216         2827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_35494_length_2827_cov_3.276696, whole genome shotgun sequence.
ACCESSION   DAOOWR010000216 DAOOWR010000000
VERSION     DAOOWR010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35494_length_2827_cov_3.276696"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLB85_05660"
     CDS             complement(<1..456)
                     /locus_tag="PLB85_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72851.1"
                     /translation="MRKIILLGLSLVLALGMFSGLFGQIVLYTWDFSSSTPNGWAVVN
                     AGSGNNWSLIGSGNAYSGTNHMQYQSHSTYAANTWAFTRSISMTAGKFYYLTFYQKVA
                     DSSRPENLKVTVGNAQTVASQTTTLLTLSSVTNTTYTQRTTAYYRPTTSG"
     gene            1116..1796
                     /locus_tag="PLB85_05665"
     CDS             1116..1796
                     /locus_tag="PLB85_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72852.1"
                     /translation="MIISTKNLIKDYSLGKVKVRALNGIDMNIEEGEFVAIMGPSGSG
                     KSTLMHIIGCLDSPTEGEYQLDGVLVSDMDKNELAMIRNRKIGFVFQSFNLLPHLNIL
                     KNVELPLMYAGIKPKERNLKAREILDSVGLSDRLKHKPAELSGGQRQRVAIARAIVND
                     PAILLADEPTGNLDSQAGGDILEIFSKLHAQGNTVIIVTHDRAVADRANRVIRIMDGK
                     IEDGDFAG"
     gene            1762..>2827
                     /locus_tag="PLB85_05670"
     CDS             1762..>2827
                     /locus_tag="PLB85_05670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU72853.1"
                     /translation="MERSKMAISLAESLSLGLADILSRKVRSIVTIIGIILGVMSIMV
                     VLAIVNGMNKTTLDWMGQRGGLNKVEVRQNWDWDLSRGGEPTFSLREIQQIRELLPPV
                     EAFNPQTQLQPQEMLYGELGYVVSMLGVFPDMEKVEDWQVAKGRFISSLDIDNHNNVV
                     VLGSTTAKELFSSRDPMGQYVGFAGQKLLVVGVMEEKYMKAQGGGSAFTDNALEYMNQ
                     RAFVPLSTLLSKIDPNQKVNSLSLQAASPEAAKELRKKVEDVVLNIKQGKKVFEVSAA
                     QEQMDQMKQSSMIFSAIFVLIAVISLLVGGIVIMNIMLASVKERTREIGVRIAVGARR
                     RDIFAQFMVQTILITALGGVL"
BASE COUNT          701 a          690 c          795 g          641 t
ORIGIN      
        1 gcccgatgtg gtgggccggt aataggctgt ggttctttga gtgtaggtag tgtttgtcac
       61 tgaagaaaga gttagcaacg tggtggtctg ggatgccacg gtctgggcat ttccaactgt
      121 gaccttcagg ttttcgggac gcgatgaatc tgcaactttc tgataaaatg ttagataata
      181 gaatttacca gcggtcatag agatgctgcg cgtgaaagcc caagtatttg ccgcgtaagt
      241 tgaatgagac tgatattgca tgtggttagt gccactatat gcatttccac tacctatcag
      301 actccagtta ttaccgctgc cggcgtttac aaccgcccag ccgttaggtg ttgaggatga
      361 aaaatcccac gtatagagta ctatttggcc aaacagacca gaaaacatcc cgagtgccaa
      421 gactaaggat aaacctaaca atatgatctt tctcataagt ttctcttttc ttaattccta
      481 atttattttc gattcaaaac atgcttcgca ttcccgttgc tgggactgga aactttgttt
      541 gtccttacat tcatggcttg ctccatgaat ctcccacaat ctacttttta gtagaattat
      601 tattggggaa aaaccagaat cgaaccaggc attcagttgt caagagaaaa atatagctct
      661 ctttcataag aggtgattac aagctgctct ctggtttagc aatgcacccg cttgatctaa
      721 aaaagagcca tatctgttta cctgtcgcaa tatcctgcca ggcaggccct gagcgcccga
      781 ttccatctct ctcgccttcc taccgtatga tcctcgcatc aagtgcgggc atcatgcggg
      841 aggcgcgact tgggcacaag aaagggcctt aacggccaga cagaaacgct tcattgctcc
      901 gcgtggtgtt cagccagacg ctggcgtcga gcgaggaact ggaatcgatg gcaacaaaga
      961 tatatggccc cgatgtatcc aagctgtagt cgaacaagga aagaggtgtt acggggaaca
     1021 cctttacaag tatcggcgga tcgaggcctt ggtgcttgtt ttttgattga cattttggag
     1081 agatatggat ttctggcttt attgagtgaa aaaatatgat aattagcacg aaaaacctca
     1141 tcaaggatta cagcctgggc aaggtgaagg tccgcgccct gaacggcatt gacatgaaca
     1201 tcgaggaagg cgagtttgtg gccatcatgg gtccttcggg ctccggaaag agcaccctga
     1261 tgcacatcat tggctgcctg gattcgccca cggagggaga ataccagctt gatggcgtgc
     1321 tggtgagcga catggacaaa aatgagctgg ccatgatccg caaccgcaag ataggcttcg
     1381 tgttccaatc tttcaacctg ctgccgcatc tgaatatcct caagaacgtg gaactgccgc
     1441 tgatgtatgc tggcatcaag cccaaagaga ggaacctcaa ggctcgggaa atcctggaca
     1501 gcgtgggact tagcgacagg ctgaaacaca agccggcaga gctttccggc ggtcagaggc
     1561 agcgcgtggc gatcgccagg gcaatcgtga acgatcccgc catcctgctg gcagatgagc
     1621 ccactggcaa cctcgattcc caggctggag gcgacattct ggagattttc agcaaactgc
     1681 acgcgcaggg caacaccgtt atcatcgtca cccacgacag agcagtggcg gaccgcgcca
     1741 accgcgtgat ccgcatcatg gatggaaaga tcgaagatgg cgatttcgct ggctgaatcc
     1801 ctttccctcg gcctggctga catccttagc cgcaaggtgc gcagcatcgt gaccatcata
     1861 ggcatcatcc tgggcgtgat gagcattatg gtggtgctgg ctatcgtgaa cgggatgaac
     1921 aaaaccaccc tcgactggat gggacagcgc ggaggcttga acaaggtgga ggtgaggcaa
     1981 aactgggact gggacctcag ccggggcggc gaaccgactt tcagcctgcg cgaaatccag
     2041 caaatccgcg agcttttgcc gccagtggag gccttcaatc cacaaaccca gttgcagcct
     2101 caggaaatgc tttacgggga actgggctac gtggtgagta tgttgggcgt tttcccggac
     2161 atggaaaagg tggaggactg gcaggtggcc aaaggccgct tcatcagctc gttggacatc
     2221 gacaaccaca acaacgtggt ggttctgggc agcaccaccg ccaaagaact tttctcctcg
     2281 cgggacccga tgggtcagta tgtgggcttc gccggtcaga aactgctggt ggtgggggtg
     2341 atggaggaaa agtatatgaa ggcgcagggc ggtggcagcg ctttcaccga caacgctttg
     2401 gaatacatga accaacgggc ttttgtgcct ctttccacgc tgctcagcaa aatcgacccc
     2461 aaccagaaag tgaacagcct cagcctgcag gcagccagcc ccgaagcggc caaggaactg
     2521 cgtaaaaaag tggaggacgt ggtgctgaac atcaagcagg gcaagaaggt ttttgaggtt
     2581 agcgcggcgc aggagcagat ggaccagatg aagcaaagct cgatgatctt ttccgccatc
     2641 tttgtgctca ttgcggtgat ttcgctgctg gttgggggca tcgtgatcat gaatatcatg
     2701 ctggcttcgg tgaaggagcg aacgcgcgaa atcggggtgc gcatcgccgt aggagccagg
     2761 cggcgggata ttttcgctca attcatggtg caaaccatcc tgatcaccgc gctgggcggg
     2821 gtgctgg
//
LOCUS       DAOOWR010000217         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_35694_length_2815_cov_3.892391, whole genome shotgun sequence.
ACCESSION   DAOOWR010000217 DAOOWR010000000
VERSION     DAOOWR010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35694_length_2815_cov_3.892391"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLB85_05675"
     CDS             complement(<1..228)
                     /locus_tag="PLB85_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72854.1"
                     /translation="MSLVTPEQVGQAYERLRELIADPAELKSKLVLVRQLLEGLYKKL
                     SADSQMSFSGLFARIQYVHERLEALPELSAQI"
     gene            complement(228..1190)
                     /gene="ftsY"
                     /locus_tag="PLB85_05680"
     CDS             complement(228..1190)
                     /gene="ftsY"
                     /locus_tag="PLB85_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU72855.1"
                     /translation="MGLISSLRKKLARTKSGFLGKIAEAVSLRGKVDEELMEQIEEIL
                     LKCDTGVEMTELIMDRLTERVRIDRITEASEVQAALQEIMEGILLKDYAEAVSLFDEI
                     DRKPYIIVFVGVNGVGKTTSIGKVADALVKAGKKVMVIAGDTFRAAAIEQLAIWTERA
                     GATLIRSKQDADPASVIYDGISSALARSFDIVLIDTAGRQHTRENLMKELTKIDRTIK
                     KLIPEAPHQALLVVDATTGQNAISQAKNFHQAMALTGLVLSKYDGTSKGGIIFNLKHN
                     LDLPVRFIGVGEGIGDLEVFDVNAFVQAFFSNTEDPEENVPGLE"
     gene            1354..2037
                     /locus_tag="PLB85_05685"
     CDS             1354..2037
                     /locus_tag="PLB85_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72856.1"
                     /translation="MNNAKFKPLMLLALLFPLLGAGLGYMLYGPGIQKGSPVVSSVPA
                     SKPGDPVLVYGPEDQLPVREARAPGSLELGIYLALVCGAVALLYHYLNRPLAWIAVIL
                     LALAFALLFHKPTGLPLTGFFLINLVLGAMLTLLVKYLFFLKALVRWRMIITTMLGAG
                     LIALYFRGLFWLTKTEFESGFWSAYFVNGILLFVFIAFGLSLADMLVQRAEIAQLRAS
                     QVSEEDEDA"
     gene            2030..2638
                     /gene="trmB"
                     /locus_tag="PLB85_05690"
     CDS             2030..2638
                     /gene="trmB"
                     /locus_tag="PLB85_05690"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU72857.1"
                     /translation="MLEDRDFFVLETPEGILDVEEPFGNRHPLHIEIGSGKGEFLSQY
                     APLHPDWNFLGFELAEKRIRNILKKLSPEKHPNVRLLKLKVDACIAELLPPVSVQSVY
                     IQHPDPWPKRRHFKRRLFQPDFLAALATVMEPEAELHIATDHEEYAHWIAEMLDRQPE
                     FVSLQSEPIQKTPSLQDHVSTWYEIEQRRQGFEPLYMLFKRI"
     gene            2652..>2815
                     /locus_tag="PLB85_05695"
     CDS             2652..>2815
                     /locus_tag="PLB85_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOU72858.1"
                     /translation="MVSLADLTRRKLWLAPLAGYTDSAFRQLCKQNGADVLVSEMVSA
                     DGLIGDSRKT"
BASE COUNT          549 a          846 c          753 g          667 t
ORIGIN      
        1 tatctgggcg gaaagttcag ggagagcttc cagccgttca tgcacatact gtatcctggc
       61 gaacagaccg ctgaagctca tctggctgtc tgcgctcagc tttttataga gcccttccag
      121 aagctggcgc accagcacca gcttgctttt caactccgcc gggtccgcga tcagttcacg
      181 caggcgttca taagcctggc caacctgctc cggggtcaca aggctcatta ttccaaaccg
      241 gggacgtttt cttccgggtc ttcggtattg ctgaaaaagg cctggacaaa ggcgttcacg
      301 tcgaaaacct caaggtcgcc gatgccctcg cccaccccga taaagcgcac gggaaggtcc
      361 agattgtgtt tcaggttaaa gatgattccg cctttggagg tgccgtcata tttgctgagc
      421 accagcccgg tgagcgccat cgcctggtga aagttcttcg cctgcgaaat cgcgttctgg
      481 ccggtggtcg cgtccactac aagcagggcc tggtgtggcg cttcgggaat gagtttcttg
      541 atggtgcggt caatcttggt gagttccttc atcaggtttt cccgcgtgtg ctggcgtccg
      601 gccgtgtcga tcagcactat gtcgaaagac ctggcgaggg cggaggagat gccgtcgtag
      661 ataactgagg ccgggtcggc atcctgtttg gagcggatca gtgttgctcc cgcccgctcg
      721 gtccagatgg ccagttgttc aatggcagcg gcgcggaaag tgtctccggc gatcaccatc
      781 actttttttc cggctttcac caaagcatcg gcaaccttgc ctatgctggt ggtcttcccc
      841 actccgttca cacccacgaa cacgatgata tagggcttgc ggtctatctc gtcgaagagg
      901 ctcaccgcct ccgcgtaatc cttgaggagg atgccttcca tgatttcctg cagggcggcc
      961 tgaacctccg aagcttccgt gatgcggtcg atcctgacgc gttcggtgag gcggtccatg
     1021 atcagttctg tcatctccac cccggtgtcg catttgagca ggatctcctc gatctgctcc
     1081 atcaactctt catccacttt gccgcgcaaa ctgaccgctt ccgctatctt gcccagaaat
     1141 ccgcttttgg tgcgggcgag tttcttcctc aaactgctga tgaggcccat tttattactc
     1201 caatcatttt caatttacct ggcctttcca gcctgcaagc aagctccgaa agcggctcag
     1261 gcgccatctg ccatatttgt gattgacagc agcgccgctt gccataaaat agctcctttg
     1321 cttagagtca ataaaaatct acgtgaggac cagatgaaca acgcaaaatt caagcccttg
     1381 atgctgcttg ccctgctgtt tccgcttttg ggggcgggtc tcggctatat gctttacgga
     1441 cccggcatcc agaagggttc ccctgtcgtt agctcagtgc ctgccagcaa accgggtgac
     1501 cctgttctcg tttacggacc ggaggaccag ctgccggtgc gggaagcgag ggcgccgggc
     1561 agccttgaac tggggatcta cctggccctg gtctgcggag cggtggcctt gctttatcat
     1621 tatctgaaca ggcctctggc gtggatagcg gtgatcctgc tcgcgctggc tttcgcgctg
     1681 ttgtttcata aacccacagg cctccccctc accggctttt tcctcatcaa cctggtcttg
     1741 ggtgcgatgc tgaccctgct ggtgaaatac ctctttttcc tcaaggcgct ggtccgctgg
     1801 cgcatgatca tcactacaat gctgggggcg ggcctcatcg cgctctattt ccgcgggctc
     1861 ttctggctca ccaaaactga gttcgaaagt ggcttctgga gcgcttactt cgttaacggc
     1921 attctcctct tcgtgttcat cgctttcggg ctgtccctcg ccgatatgct cgtccagcgc
     1981 gctgaaatcg cccaattgcg ggccagtcaa gtctcggaag aggatgagga tgcttgaaga
     2041 ccgggatttt ttcgttctgg aaaccccgga aggcatcctg gacgtggaag agccgtttgg
     2101 taaccgccac ccgcttcaca tcgagatagg ttccggcaag ggggaattcc tctcccaata
     2161 cgccccgctg catccggact ggaacttcct cggttttgaa ctggctgaaa aacgcatccg
     2221 caacatcctg aaaaagcttt ccccggagaa gcatcccaac gttcgcctgc taaagctgaa
     2281 agtggatgcc tgcatcgcgg aactgctgcc ccctgtttcc gtgcagagcg tttacatcca
     2341 gcatccggac ccctggccca agcgcaggca tttcaagcgc cgcctcttcc agcctgattt
     2401 cctggccgcc ctggccacgg tgatggagcc ggaagccgag ctgcacatag ccaccgacca
     2461 tgaggaatac gcccattgga tagctgaaat gctcgacagg cagccggaat ttgtgtctct
     2521 gcaatccgaa cccatccaaa aaacgcccag cctccaggac cacgttagca cctggtatga
     2581 gatagaacag cgccgtcagg gctttgagcc cctctatatg ctgttcaagc gcatttgaga
     2641 aaatccccct catggtcagc ttagccgacc tcacccggcg caaactctgg ctggcccccc
     2701 tggccggcta cacagacagc gctttccgcc agctttgcaa acaaaacggc gctgatgtgc
     2761 tcgtgagcga gatggttagc gccgacggac tcatcggcga ttccaggaaa acgga
//
LOCUS       DAOOWR010000218         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36137_length_2789_cov_3.643014, whole genome shotgun sequence.
ACCESSION   DAOOWR010000218 DAOOWR010000000
VERSION     DAOOWR010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36137_length_2789_cov_3.643014"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..1531
                     /locus_tag="PLB85_05700"
     CDS             425..1531
                     /locus_tag="PLB85_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOU72859.1"
                     /translation="MQALFGGILAFQWQQAVMILIGLVLIWLAIAKKYEPTLLLPIGF
                     GTILTNIPFSAAVGEHGPLQILFNAGIATELFPLLIFIGIGAMIDFTPLLKNPFMLLF
                     GAAAQLGIFVTFSAATLLGFSLKQAGAIGIIGAADGPTSIYVANRFAQDLLPAISVAA
                     YSYMALVPIIQPPVIKLLTSKEERKIRMDYQAGEVPKVVKIIFPIAITLIAGIFVPAS
                     AALIGFLMFGNLIRECGVLESLSKSAQNELANLVTIFLGISIASTMRGEAFLRPQTLL
                     IFILGLVAFIFDTAGGVLFAKFINLFRKNKINPMIGACGISAFPMSARVIHQMGQKED
                     PFNFLLMPAVSVNVGGQIGSVIAGGLILALLGNA"
     gene            1528..1920
                     /locus_tag="PLB85_05705"
     CDS             1528..1920
                     /locus_tag="PLB85_05705"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOU72860.1"
                     /translation="MSIDLDALLEQAQAAMEKAYAPYSGFRVGAALLCADGSVVTGCN
                     VENSSYSLTICAERNALFAAVNQGKRDFLAIAIYVDSDEMFPPCGACRQVLAEFSPRL
                     QILYANRLGSISSDLESLLPQAFSLREE"
     gene            1917..>2789
                     /locus_tag="PLB85_05710"
     CDS             1917..>2789
                     /locus_tag="PLB85_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU72861.1"
                     /translation="MISSHPELLHCRVCPQNCGVNRYEATGFCGAGASIKVNSAHLHH
                     GEEPVLSGTGGSGTIFLSYCNLRCVFCQNHQISHLGWGSEISEEDCAGMMLRLQEAGA
                     HNVNLVSPTQYTPQLATAIKLARAGGLSIPVVWNSNAYETVESLQTLAGLVDIYLPDL
                     KYAHPAYSLTYSQARDYPAISLKAVQVMQSQVGKLVISNSGLALRGLLVRHLVLPNRL
                     AGTRDLLYTLRETLGEGVSLSLMAQYYPTAAAQNIPELARGLHPEEYAEALDLAMNLG
                     FTDIYAQELDPSPDW"
BASE COUNT          603 a          795 c          742 g          649 t
ORIGIN      
        1 tggaatagga tgaattcatc tcaattcaaa tgcgggtgta ttattcaacg gtcttggata
       61 tgttgtgaaa atcgtccact gctgccgtcg atgcttgtca cttgctcgac tccagcgtat
      121 aatcagggag aagcgacgcc gtctggccgt taaggccttg ccctgtgttc ctgaagcgcc
      181 tcccgcacca tgcccgcatt tgatgcgggt atcatgcgag aggcgtggga gagggacgga
      241 cgcaggcgct cagggtgtaa ccgacggaag attacgaaga atgagcagga aaagcaacat
      301 ctgccggtaa tactcatcga aggagccaaa ctgaccgcga gagcgtgtgg acggatatat
      361 tcattgacaa agaggacagc gccggaattt atggattttt gagtgaaaat atctggagtt
      421 attgatgcaa gcattatttg gtggaatcct ggccttccag tggcaacagg cggtgatgat
      481 cctgatcggc ttggtgctga tctggctggc catcgcgaaa aaatatgagc cgaccctgct
      541 gctgccaatt gggtttggga ccattctcac gaatataccc ttctccgcgg ctgtgggaga
      601 gcacggacca ctgcagatcc tgttcaatgc aggcatcgcc acagagcttt tcccgctgct
      661 gatcttcatc ggcatcggcg ccatgataga cttcacgccc ctgctcaaaa accctttcat
      721 gctacttttc ggcgcggcgg ctcaactggg tatcttcgtg accttttccg ctgccaccct
      781 gcttggcttc agcctgaaac aggctggcgc aatcggcatc atcggcgcgg ctgacggccc
      841 cacctcgatt tacgtggcca accgtttcgc gcaggacctg ctgccggcga tttccgtggc
      901 tgcctattcc tacatggctt tggtgcccat catccaaccg cctgtgatca aactgcttac
      961 ctccaaggaa gaacgcaaga tccgcatgga ctaccaggcc ggagaagttc ccaaagtggt
     1021 aaaaatcatc ttccccatcg ccatcacgct gatcgccggg atttttgtgc cggcttcagc
     1081 ggcgctgatc ggctttttga tgttcggtaa cctgatccgc gaatgcggcg tgctggaaag
     1141 tctgtccaaa tcagcgcaga atgaactggc caacttggtg acgatctttc tgggcatcag
     1201 catcgcctcc acgatgcgcg gagaggcttt cctgaggccg caaacccttc tgatattcat
     1261 acttgggctg gtggccttca ttttcgatac cgccggcgga gtgctcttcg caaaattcat
     1321 taaccttttc cgcaagaaca agataaaccc gatgatcgga gcctgcggga tatcggcctt
     1381 tccgatgtcc gcgagggtta tccatcagat ggggcaaaaa gaagacccct tcaacttcct
     1441 gctgatgcca gccgtgagcg ttaacgtggg cggccagatc ggttctgtga tcgccggcgg
     1501 cctcattctg gcgctgttgg ggaatgcatg agcattgatc ttgacgcctt gctggagcag
     1561 gcccaggccg cgatggaaaa ggcttacgcg ccttattccg ggttccgggt gggggccgct
     1621 ctactctgcg ctgatggcag cgtggtcaca ggctgcaacg tggaaaattc atcttattcc
     1681 ctgaccattt gcgccgagcg caacgccctc ttcgccgctg tgaatcaagg caagcgggat
     1741 ttccttgcca ttgccatcta tgtggattct gatgaaatgt tccctccctg cggtgcttgc
     1801 cgtcaggttt tggcggaatt cagcccccgc ctgcaaatcc tctatgccaa tcgccttggc
     1861 agcatcagca gcgacctgga atccctcctg ccccaagcat ttagcctaag agaagaatga
     1921 tttccagcca tcccgaattg ctccattgcc gggtctgccc tcaaaactgt ggcgtgaacc
     1981 gttacgaagc cacaggattc tgcggagcgg gagcatcaat caaggtaaac agcgcgcacc
     2041 tgcatcatgg cgaggaacct gttctcagcg gcaccggagg cagcggcacc attttccttt
     2101 cctattgcaa ccttcgctgc gttttttgcc agaatcacca gatatcacat ctgggctggg
     2161 gcagtgagat cagcgaagag gattgcgccg ggatgatgct gcgtttgcaa gaagccggag
     2221 cccacaacgt aaatctggtc tcaccaacgc aatacacacc tcagcttgct acagcgataa
     2281 agctggctcg ggccggaggc ctctctattc cagtggtttg gaacagcaac gcttatgaaa
     2341 ccgtcgaatc ccttcaaacc ctggccgggc tggtggacat ctatttgcct gacctcaaat
     2401 acgcgcatcc agcctattcc ctaacatact cccaagcaag ggattatcca gccatatcct
     2461 tgaaagccgt gcaagtaatg cagagccagg taggtaagct tgtgatatcc aatagcggat
     2521 tggcccttcg cgggcttctg gtacgtcatc tggtgctgcc caaccgtctg gccggcacgc
     2581 gtgacctgct ctacaccctg cgcgaaaccc tgggcgaggg tgtttctctc agtctgatgg
     2641 cccaatacta tcctacagcc gcggctcaaa acatccccga actggcccgc ggtcttcatc
     2701 ccgaggaata cgccgaggcg ctggatctgg ccatgaacct tggttttacc gatatttacg
     2761 ctcaggaact cgacccctca ccagactgg
//
LOCUS       DAOOWR010000219         2771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36437_length_2771_cov_3.783505, whole genome shotgun sequence.
ACCESSION   DAOOWR010000219 DAOOWR010000000
VERSION     DAOOWR010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36437_length_2771_cov_3.783505"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLB85_05715"
     CDS             <1..289
                     /locus_tag="PLB85_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOU72862.1"
                     /translation="YFSERQQLRGIIVLVDIRHEADPKDVQLLELLRLREIDHCVVAT
                     KADKIPKTKVARTLDLLGKGLGLQGMPLFPVSALKNTGLAPLYSWLGRHLN"
     gene            294..2138
                     /gene="mnmG"
                     /locus_tag="PLB85_05720"
     CDS             294..2138
                     /gene="mnmG"
                     /locus_tag="PLB85_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU72863.1"
                     /translation="MPPSFDVIVVGGGHAGVEAALAAARRGAKTALFILKLESLARLS
                     CNPSLGGPAKGHLAREIDALGGELGYVADLSGIHFRMLNRGKGPAVWAPRSQNDRQLY
                     SRLMLRHAEAQDGLLLIEADVAEILVERGRALGVRTVVGTEYRAPQVILATGTFLRGL
                     IHVGKVSIPGGRSGEPSSEALSGSLDRLGFRLARFKTGTPPRVDMRSLDYSCLEEQPG
                     DPEPEGFSWYRDVNLSNQVSCYLTRTTPETHRIIRENLQLSSLYSGIIKGIGPRYCPS
                     IEDKIVKFPQRESHQIFIEPEGLNTFEGYVNGVSTSLPPEVQEAVVHSIPGLEQARIM
                     RHAYAIEYDFIEPSELSPSLESRRVKGLWLAGQINGTSGYEEAAAQGIMAGINATLAL
                     EGQPPLTLSRGEAYIGVLIDDLVTCGTNEPYRMFTSRAEYRLLLRQDNCDERLMPLGY
                     KLGLVSDQRWRRFQRMLELKERELERLRSEKSSSHPELREPQKFAALLKRPDTNWARL
                     AEFGYTPSPEVTPDILRRCELEIKYEGYLKRAEEEMRRFQASEELALDEDIDYRAIET
                     IAWEAREKLQRVKPRSLGQANRIPGVNYTDTAALLVWLRKRQKTAPDG"
     gene            complement(2148..>2771)
                     /locus_tag="PLB85_05725"
     CDS             complement(2148..>2771)
                     /locus_tag="PLB85_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72864.1"
                     /translation="YESGFFSRLHLDSRFVLLANASLARIPDLADNLYDVYNGMLGCT
                     WARLSNLVIYAGYMYGNNLVYQLSGTYPQHGVNINAWAAPLKNMSLSLVGSWNKFGYD
                     KLTDPDTGQVRLDDEYIIANATLEYTPTAVFKVTLGSGFSSYELAGSSATLNYYGNLR
                     YEFRPEWFAFVGIKSAQTQTEASTWNNPTGEFQRNLHTAYAKLSVTF"
BASE COUNT          565 a          850 c          840 g          516 t
ORIGIN      
        1 ctatttcagc gagcgccagc aactgcgcgg aatcatcgtg ctggtggaca ttcgccacga
       61 agccgacccc aaggacgtcc aactgctgga attgctgcgc ctgcgcgaga tcgaccactg
      121 cgttgtggcc accaaggcgg acaagattcc caaaaccaaa gtggccagga ccttggattt
      181 gctgggcaaa gggctgggcc tgcaaggaat gccgctgttc ccggtctcgg ccctcaaaaa
      241 caccgggctg gctccgcttt actcctggct tggcaggcat ctgaactaag gccatgcccc
      301 ccagcttcga tgtgatcgtg gtcggcggag gccacgccgg ggtcgaggcg gccctggccg
      361 cggcgcgcag gggggcgaaa acagcgctct ttatcctcaa gctcgaatcc ctcgcccgct
      421 tgagctgcaa tccctcactc ggcggtcccg ccaaaggcca tctggccagg gaaatagacg
      481 ctctgggcgg tgaattgggc tatgtggccg acctttccgg catccatttc cgcatgctca
      541 accgcggcaa aggccccgct gtttgggccc cgcgtagcca gaacgaccgc cagctctatt
      601 cccggctgat gctccggcac gcggaggcgc aggacgggct gctgctgatc gaggcggacg
      661 tggcggaaat cctggtggag cggggacgcg cgctgggagt cagaaccgtt gtcggcaccg
      721 aataccgggc tccacaggtg attctcgcca ccggcacctt tttgcgcggc ctcatccacg
      781 tgggcaaggt ttccatcccc ggcggacgca gcggcgaacc ctcttccgaa gcgctttcgg
      841 ggtcattgga cagactgggc ttccggctgg cacgcttcaa gacgggcact ccgccccggg
      901 tggacatgcg cagcctggac tattcctgcc tggaggaaca gcccggcgat cccgaaccag
      961 agggattttc ctggtaccga gacgtgaacc tttccaacca ggtgagctgt tacctgaccc
     1021 gcacaacccc ggaaacgcac agaattatcc gggaaaacct gcagctttcg tcgctttaca
     1081 gcggcatcat caagggcatc gggccccgct actgcccctc catcgaggac aagatcgtca
     1141 aatttcccca gcgcgaaagc caccagatct tcatcgaacc ggagggcctg aacacctttg
     1201 agggttatgt gaatggcgtt tccacctccc tgccgcccga agtgcaggaa gccgtggtcc
     1261 acagcatccc ggggctggaa caagcccgga tcatgcgcca cgcctatgcc atcgaatacg
     1321 atttcatcga gccctcggaa ctcagcccca gcctggaaag caggcgcgtc aaggggcttt
     1381 ggctggcagg gcagataaac ggcacctcgg gatacgaaga ggctgccgca cagggcatta
     1441 tggccggcat caacgccacc ctcgccctgg agggccaacc tccgctcacg ctcagccgcg
     1501 gcgaagccta cattggcgtt ctgattgacg atttggtcac ctgcggcacc aacgagccct
     1561 accggatgtt cacctcccgg gctgaatacc gcctgttgct gcgccaggac aattgcgacg
     1621 aacgcctgat gcccctgggt tacaaattgg gtttggtttc tgaccagcgc tggcggcgct
     1681 tccaaaggat gctggagctg aaggaaaggg aattggagcg cctgcggagt gaaaaatcct
     1741 catcccatcc cgaactgcgc gaaccccaga aattcgccgc cctgctcaag cgtccggata
     1801 caaactgggc taggctggcg gaattcggct acaccccctc gccggaggtc acgccggaca
     1861 tcctgcggcg ctgcgagctg gaaatcaaat atgagggcta cctcaagcgg gcggaagagg
     1921 aaatgcggcg ctttcaggcc tctgaggaac tggctttgga cgaggacatc gactaccgag
     1981 ccatcgaaac catcgcctgg gaggcccgcg aaaagctgca gcgcgtgaaa ccccgcagcc
     2041 tgggccaggc caaccgcatt cccggggtga attacaccga cacggcggcc ctgctggtct
     2101 ggctgcgcaa aagacagaaa actgctccgg acggataacc cggccgctca gaaagtcacg
     2161 ctaagcttgg cgtaggcggt gtggaggttc cgctggaatt ccccggtcgg attgttccag
     2221 gtggaagctt cggtctgcgt ttgggcggat ttgatgccga cgaaggcgaa ccattccggc
     2281 cggaattcgt agcgcaggtt tccatagtag ttcagggtcg cgctgctccc agccaattca
     2341 tagctgctga acccgctgcc cagggtgacc ttgaaaacgg ctgtgggcgt gtattccagg
     2401 gtggcgttgg cgattatgta ttcatcgtcc agccgcacct gcccggtatc gggatcggtc
     2461 agtttgtcgt aaccgaactt gttccagctt cccacaaggc tcagggacat gtttttcagg
     2521 ggggcggccc aggcgttgat gttcacaccg tgctggggat atgtgccgct gagctggtac
     2581 accaggttgt ttccatacat atagccggca tagatcacga ggttgctcag tctggcccag
     2641 gtgcagccca gcatgccgtt gtaaacgtcg taaaggttgt ccgcgaggtc ggggatccgg
     2701 gccaaacttg cgttcgccag taaaacgaag cgggaatcca ggtgaagtct ggagaaaaac
     2761 cctgattcat a
//
LOCUS       DAOOWR010000220         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36568_length_2764_cov_3.479513, whole genome shotgun sequence.
ACCESSION   DAOOWR010000220 DAOOWR010000000
VERSION     DAOOWR010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36568_length_2764_cov_3.479513"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLB85_05730"
     CDS             complement(<1..842)
                     /locus_tag="PLB85_05730"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOU72865.1"
                     /translation="MQTEFKQIDPTTKEIVLTVEAEKVDAAYQKYLQKAANSLEVPGF
                     RKGKAPLSMVTRLHGDRIKDYFEKDYVDEAFSEAAKEHDIRFLLYPEVKELEWQQGSE
                     MKITLEIEHEPPVEFRQIDNLQVPFKPLVLEEEVEKFIQKLSTEHATFQHIDVAETGD
                     LVLGHLSFELNGTAHTMETTFYADDKETEGFPGKIVGASTGDRLEAELDGKNLKLMLP
                     NDHGLDVDEEAKYACSFETSDITRNVVPAIDDEFAKDMDFADLTEMRAKIGEDMKTRI
                     EHVN"
     gene            complement(860..945)
                     /locus_tag="PLB85_05735"
     tRNA            complement(860..945)
                     /locus_tag="PLB85_05735"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(909..911),aa:Leu,seq:tag)
     gene            complement(1011..1850)
                     /gene="panC"
                     /locus_tag="PLB85_05740"
     CDS             complement(1011..1850)
                     /gene="panC"
                     /locus_tag="PLB85_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU72866.1"
                     /translation="MRVCKTVAEVRQALAEARQGRKVGFVPTMGALHRGHLSLVERCA
                     METDLTVVSIYVNPAQFGPKEDLGKYPRDLEGDLELLSQHRTDLVFFPNNEQMYPQGY
                     RTWVEVEGLSNILCGASRPGHFRGVATVVLKLTQIVKPDLMFMGLKDFQQIVVLERML
                     QDLNSETRIARCPIVREEDGLAMSSRNAYLSPEERVQARCLSQAWKQARETVAQGCRN
                     SLELLDQAEKTILEAGGQIDYIKIVDSDTLSDVDIVDENSRMLMAVKIGNTRLIDNSA
                     LLA"
     gene            complement(1850..2200)
                     /locus_tag="PLB85_05745"
     CDS             complement(1850..2200)
                     /locus_tag="PLB85_05745"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU72867.1"
                     /translation="MLRYKLLSKLHRATVTECDLNYNASIQIDSELLQLSGMHEHERV
                     EIWDVNNGARFSTYIIAAEPGSRKISINGAAARMVHTGDKIIVVNYGLMDEKEMETIQ
                     PKIIILGEGNEPVK"
BASE COUNT          562 a          758 c          680 g          764 t
ORIGIN      
        1 taattgacat gctcgatcct ggttttcatg tcttcaccga tcttggcacg catttccgta
       61 aggtcggcaa agtccatgtc ctttgcaaat tcgtcatcga tggctggcac cacgttgcgg
      121 gtgatgtcgc tcgtttcaaa gctgcacgcg tatttggctt cttcgtccac atccagccca
      181 tggtcattag gcagcatcaa tttcaggttt ttgccgtcca attccgcttc cagcctgtca
      241 cctgtactgg cgcccacgat tttgccgggg aacccctccg tctccttgtc atctgcgtaa
      301 aaggttgttt ccattgtatg tgcggtgccg ttcagctcaa agctcagatg ccccaacacc
      361 aagtctcctg tttcggccac atcaatgtgc tggaaggtgg cgtgttcggt gctcagtttc
      421 tggatgaact tctccacctc ttcttccaga accagcggct tgaagggcac ctgcagattg
      481 tctatctgcc tgaattccac cggcggttcg tgctcgatct caagggttat cttcatttcg
      541 gagccctgct gccactccag ttctttcact tccggataga gcaggaagcg gatgtcatgt
      601 tccttcgctg cttcggagaa agcttcgtcc acatagtctt tctcgaaata gtccttgatc
      661 ctgtcgccgt gcagccttgt caccatgctc agcggggctt tgcctttgcg gaatcccggc
      721 acttccagag agttggctgc tttctgcagg tatttctggt aggccgcgtc cactttttcg
      781 gcttccacgg tgagcactat ctctttggtg gttgggtcga tctgtttgaa ttctgtttgc
      841 actttgtctc ctgaatatat ggtgcgaaag aggggacttg aacccctacg ggattatccc
      901 actggatcct aagtccagcg cgtctgccta ttccgccact ttcgcgaatg aataatagaa
      961 ttaatgaatt gcatgtataa tgggtcaagt ttttccggcc cgacccgctg tcaggcaagt
     1021 agtgccgaat tgtctatcag ccgcgtgttg cctatcttca cagccatcaa catgcgtgaa
     1081 ttttcgtcca caatgtccac gtcggacagg gtgtcggagt ccacgatttt gatgtagtct
     1141 atctggccac cggcttccag aattgttttt tcagcttgat ccaataattc aaggctgttc
     1201 cggcagccct gtgccacagt ttctctcgcc tgtttccagg cttggctaag gcatctggcc
     1261 tggacgcgtt cttcggggct taaatacgcg ttgcgcgaac tcatcgccag tccgtcttcc
     1321 tctctaacga tgggacagcg cgcgattcgg gtttcgctgt tcaggtcctg cagcatgcgc
     1381 tccagcacca ctatctgttg aaaatccttc aaacccatga acatcaggtc aggtttcact
     1441 atctgggtca gcttcagcac cacggttgcc acaccccgga aatgaccggg ccggctggcg
     1501 ccgcagagaa tattggaaag gccctccacc tcaacccagg ttctgtaacc ctggggatac
     1561 atctgctcgt tgttgggaaa gaataccaga tccgtccggt gttgggaaag cagttccaga
     1621 tcgccttcca ggtcacgggg atatttgccc agatcttctt tcgggccgaa ctgggcggga
     1681 ttcacgtaga tcgaaaccac tgtcaggtcc gtctccatcg cgcaacgctc caccagggaa
     1741 agatggccgc ggtgcagggc gcccatcgta ggcacaaagc cgactttccg gccttgcctg
     1801 gcctctgcca gagcctggcg gacttccgcg accgttttgc aaacccgcat tatttaaccg
     1861 gttcgttgcc ctcgcccaaa ataatgatct tgggctggat ggtttccatt tccttctcgt
     1921 ccatcaggcc gtagttgacc acgatgatct tgtcgcctgt gtgcaccatc cgggcagcgg
     1981 cgccgttgat gctgatcttg cggctgccgg gttccgcggc gatgatgtag gtggaaaacc
     2041 tcgccccgtt gttcacgtcc cagatctcca cgcgctcgtg ctcgtgcatg ccgctaagct
     2101 gcaaaagttc gctgtctatc tggatgctgg cattgtagtt cagatcgcac tcggtcacag
     2161 tagcccggtg cagcttggaa agaagtttat acctgagcat tttcacactc cctaatttgt
     2221 ttagaataat cccaaaaaat acaggcagta tatatgtcaa tgaatttcgt taacctttgg
     2281 gtggtccaaa gcctcccgat ctggattcct gcctccttct tagcggtctc gaccattccc
     2341 ctcgcatgcc tctcgccaaa tccccgtatc aaatgcgggt atcgtgcggg aggcgagact
     2401 ggtgcctggg aaaaggcctt aatggccagt aggcattgct tcactctttc aaggatcgcc
     2461 agcttgggat gacaatagtc gcgccactct atttgaactc aagccatatt ttctttccat
     2521 tctttaagca tactgcagat aagcagataa aatgagaggc tgtcacaggg gcggaatatt
     2581 ttcaaaaatt tgtgttgaca caaaaaccgc tgtttttttc atggctatcc tgcgcggttg
     2641 agatgctgcg caaattgagt tctttaaaag catatagagt gccatagaga tttgtgcgag
     2701 ccaacagaag tcaattcatt catattatcg atatgatgga gagtttgatc ctggctcagg
     2761 acga
//
LOCUS       DAOOWR010000221         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36663_length_2759_cov_3.476701, whole genome shotgun sequence.
ACCESSION   DAOOWR010000221 DAOOWR010000000
VERSION     DAOOWR010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36663_length_2759_cov_3.476701"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLB85_05750"
     CDS             complement(<1..829)
                     /locus_tag="PLB85_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72868.1"
                     /translation="MKKPALILLVSALASLSAAPRGLTSREIVIEDAVVVVQPEAVLT
                     IPAPAPANPGSGLRENWCPLLEMSLGFVLPAGEQFGIYFDNKEQFANPYFTGGSVVNE
                     SHYRQAVAKAPTWIQAELHKTLLQLDYYPRMHFSTLIINAPDPIVDEIAFCVATASPQ
                     YLNSSFAYPELFTENAEWIYSVASQLPYVEIVDVGSAATGGDYYSTTRYWKKDANGQF
                     QQVTVPREIYYWYLVNPKITDEIQAYIDPNIVENNSTHSNNIAAPWNGGKFWRSYFYD
                     "
     gene            complement(1145..2362)
                     /locus_tag="PLB85_05755"
     CDS             complement(1145..2362)
                     /locus_tag="PLB85_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU72869.1"
                     /translation="MSVFGILRIALKSLTRNKTRTFLTMLGIIIGVAAVITMIAIGQG
                     AKKVVDDQISTMGTNVVSVMSNFTNTQSTARQAAGSGNTLEEEDVDAILDKVDGVLYA
                     SPVYNTFGQLKYGNNNWRGRIMGVDVDYFLIRDMKMESGDFFYPSEVESGAKVCVLGK
                     TVADNLFVGEDPVGKIMRIRNIPFTVKGVLKSKGQGTMGNDQDDIVLAPFTTTSTRLI
                     GHRWRFLTIIVSAVSQERIPIVQEDISNLMQARHSGTTAADFMVSSQTDVAEAANSVS
                     NTMTVLLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGAGKRDVLLQFIIEA
                     ITISILGGLIGIVLGWGLAVILGKTMGWSIVVTSWSILLSVGFSCVIGIFFGWYPARK
                     AANLNLIDALRYE"
     gene            complement(2362..>2759)
                     /locus_tag="PLB85_05760"
     CDS             complement(2362..>2759)
                     /locus_tag="PLB85_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU72870.1"
                     /translation="DRRAMALKALETVGLHDRARHFPNQLSGGQQQRVAIARAIVNDP
                     VFLLADEPTGNLDTRTSVEVMEFFQKLHAEGKTIILVTHEFDISQYATRIITFRDGRI
                     ISDLPNPDPRNATEDLKKLPRLDDSEEVA"
BASE COUNT          604 a          726 c          820 g          609 t
ORIGIN      
        1 agtcgtaaaa atatgagcgc cagaattttc ccccattcca gggagctgcg atattgttgg
       61 aatgggtgct gttgttttcc acaatgttgg gatcgatata ggcctggatt tcgtcagtta
      121 tcttgggatt caccagatac cagtaataga tctccctggg gacggttacc tgctgaaatt
      181 ggccattggc gtcctttttc cagtagcggg tggtggaata gtaatccccg ccggtggcgg
      241 cggaacccac atccactatt tccacatagg gcagttggga ggcaacggaa tagatccact
      301 cggcgttttc ggtgaacagt tccgggtaag cgaaactgga gttcagatac tggggcgagg
      361 ctgtcgccac gcagaaagcg atctcgtcca cgatggggtc cggcgcgttg attatcaggg
      421 tggagaaatg catccgggga taataatcaa gctgcagcag cgttttgtgc agttcagcct
      481 ggatccaggt cggagccttg gccacggctt gtctgtaatg cgattcgtta accacactcc
      541 cgcccgtgaa atagggattg gcaaactgtt ccttgttgtc aaaatagatg ccaaactgct
      601 ctccggccgg aagcacaaaa cccagcgaca tttccagcag tgggcaccag ttctcgcgga
      661 gcccgctccc cggattagca ggcgctggag cggggatagt caaaaccgct tccggctgaa
      721 ccacaaccac cgcgtcctcg ataactattt cccggctggt gaggccgcgc ggagcggcac
      781 tcagagaggc cagggcggaa accaaaagaa tcagggcagg ctttttcatc gaatcacctc
      841 gatcaaaata ctcttatgct cataattatt tcccgcccaa tgctgtcaat gaaaaagggt
      901 ttgaaccaac ttaatgcctc cttagcgccc gaacctatcc ctctcactcg cccctcgcca
      961 aatccccgca tcagatgcgg ccatcatgcg ggaggcacat ctggagccca atacagggcg
     1021 ttaacggcga ggtggtatag attcaatcct ctgaggattt cccagccgct ttatatcctc
     1081 gcaacggagt ggcgcccttc gggttgaacc attcgatttg aaaaagagcc taaaaaaaga
     1141 ggcactattc gtagcggagg gcgtcgatga ggttcaggtt cgcggctttg cgggcaggat
     1201 accagccaaa aaagatgccg atgacacagg aaaagccgac ggagagcagg atcgaccagg
     1261 aggtcaccac gatgctccag cccatggttt tgcccaggat cacggccagt ccccagccca
     1321 ggacgatgcc gattaacccg ccgaggatgc tgatggtgat ggcttcaatg atgaattgga
     1381 gcaatacatc gcgctttccg gcgcccacgg ccatgcggat gccgatttcc ttgatccttt
     1441 cagtaaccga gaccagcatg atgttcataa tgccgatccc gcccacgagc agcgaaatcc
     1501 cggcgatgct ggccagaagc acggtcatgg tgttggaaac gctgttggcg gcctcagcca
     1561 cgtcggtttg ggaactcacc atgaaatctg cggcggtggt gccgctgtgg cgggcttgca
     1621 tgaggttgga gatatcttcc tgaacaatgg ggatgcgttc ttgcgaaacg gcggaaacga
     1681 tgatggtaag gaagcgccag cggtgtccga tgagccgggt ggaggttgtg gtaaaaggag
     1741 ctaggacaat gtcgtcctga tcgttgccca tagtgccctg gcccttggat ttgagcacgc
     1801 ctttcacggt gaaggggatg ttgcggatgc gcattatctt gcccactggg tcctcaccca
     1861 cgaacaggtt gtcggccacg gttttgccca gcacacagac cttggcaccg ctctccacct
     1921 cggaggggta gaagaagtcg cccgattcca tcttcatatc gcggatcagg aaataatcca
     1981 cgtccacacc catgatgcgt ccgcgccagt tgttgttgcc gtatttaagc tggccgaaag
     2041 tattgtaaac cggtgaggcg taaagaactc cgtccacttt gtcgaggatg gcgtccacgt
     2101 cctcctcttc gagggtgttg ccggaaccgg cagcctggcg cgcagtgctt tgggtgttcg
     2161 tgaaattgga catcacggag accacattgg tgcccatggt actgatctgg tcgtccacca
     2221 cctttttggc gccctggccg atggcgatca tggtgatcac ggcagccacg ccgatgatga
     2281 tgcccagcat ggtcagaaag gtgcgggttt tattgcgggt tagggattta agggcgatgc
     2341 ggagaattcc gaaaacagac atcaggccac ctcctcgctg tcatccaggc ggggcagctt
     2401 cttcaggtct tcagtggcat tgcggggatc gggattgggc aaatcggaga tgatgcgccc
     2461 gtcgcggaaa gtgatgatcc gggtcgcgta ttgggagatg tcgaattcgt gggtcaccag
     2521 gatgatggtc ttgccctcgg cgtgcagttt ctgaaaaaat tccatcactt ccacgctggt
     2581 gcgggtgtcc agattgcccg tgggctcatc cgcgagcagg aacacggggt cgttgacgat
     2641 ggcgcgggcg atggcgacgc gctgctgctg tccgccggaa agctggttcg ggaaatggcg
     2701 ggctcggtca tgcaaaccta ctgtttcgag agctttgagg gccatggcgc ggcggtctt
//
LOCUS       DAOOWR010000222         2746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36896_length_2746_cov_4.905983, whole genome shotgun sequence.
ACCESSION   DAOOWR010000222 DAOOWR010000000
VERSION     DAOOWR010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36896_length_2746_cov_4.905983"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLB85_05765"
     CDS             complement(<1..222)
                     /locus_tag="PLB85_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72871.1"
                     /translation="MFSARRVIDFSVVGKWHISAEDHWYIVGEDLWRSVGEYFWYIVG
                     ECIWYIIGRLVTPDRSDGQDPGDYGFAGPF"
     gene            240..1787
                     /gene="istA"
                     /locus_tag="PLB85_05770"
     CDS             240..1787
                     /gene="istA"
                     /locus_tag="PLB85_05770"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOU72872.1"
                     /translation="MSERRLNLMDIREMIRHIREERSDRQIGKDLGVDRRTVKRYRIW
                     AQEQGLLTGELPDHEKLLKLLDETMPEKVPPQNSSKADPYRGRIETLLKEKVKVTAIF
                     DRLREQGYQGSYASVLRLARQIVPKPVEAFTRKECQPGEEAEIDFGYVGMMLDTEGNL
                     RKAWAFVMVLGWSRYAYVEFVFNQKAETWLRCHRNALEFFGGVPKRLVIDNLKSGITQ
                     AIWDDPVVQMAYRECAEHYGFLILPCRPVTPEHKGKVEGGVGYVQGRFMGGRGPMSLT
                     QANQEVRKWCQGEAGMRIHGTTKEQPRRQFETVEKGHLKPLPTTPYDLAIWKKAKLHR
                     DCHVVFEGSYYSAPFRLVGQHLWVCAGTQQVRLFNAKYELVATHERAAKPGTRRTHRD
                     HYPPEKLPGLERTREGCLEQAAALGPAIRQTVEQILADPVLDRIHQAGRLLRLSEKYG
                     CQRLNDACQRALDFGDPAYKTVKGILKQNLDQEEPPIPVQLSPALTFSRQPDELVGAL
                     AEVPTWN"
     gene            1778..2551
                     /gene="istB"
                     /locus_tag="PLB85_05775"
     CDS             1778..2551
                     /gene="istB"
                     /locus_tag="PLB85_05775"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOU72873.1"
                     /translation="MELKPQLSHQLRQLRMSGVMETLDARNRQAIDGQWSYQEFLSRL
                     LEDETERRAQKQLSMRLRRSTLNATKTIEGFDFQFNPNLNRQQILQLAACEYLREHRN
                     LLICGPTGVGKTHLAQALGQEAARQGFDVLFVSVQKMLQHLNGGRADGSWERRLGNYL
                     RPDLLILDDFGLKPIQPPGPEDLYDVINERYERGSILLTSNRAPNEWPDLFLDPLLAS
                     AGLARLLDRAEVVIIRGASYRAQGRARLTEEVPTAQKQP"
BASE COUNT          674 a          751 c          808 g          513 t
ORIGIN      
        1 aaatggacct gcaaagccat aatccccagg atcctggcca tcagaacgat caggtgtcac
       61 taaacgccct ataatgtacc agatgcattc acccacgatg taccagaaat attcacccac
      121 actgcgccac agatcttcac caacgatgta ccagtgatct tcagccgaaa tgtgccactt
      181 tccgacgacg gaaaaatcga ttacccttct ggcagaaaac aaaacagcca ggaggaacca
      241 tgtcagaaag aaggctgaac ctaatggaca tacgagaaat gattcggcac atccgggaag
      301 aacggagtga ccggcaaatc ggcaaagatt taggggtaga ccggcgaacg gtgaaacgct
      361 accggatatg ggcgcaggaa cagggattac tgaccggcga actgccggat cacgaaaagc
      421 tgctgaaact actggatgag accatgccgg agaaagtacc gccgcaaaac agctcaaaag
      481 cggatccgta tcgtggacgg atagaaacgc tgctcaaaga gaaagtgaaa gtgacagcca
      541 tctttgatcg gctgagagaa caagggtacc agggcagtta tgcttcagtg ctgaggctgg
      601 cgcgccaaat tgtgccaaag ccagtggagg catttacgcg caaggaatgc cagcctggag
      661 aagaagcgga aattgacttt ggctatgtgg ggatgatgct ggacacagaa ggtaatttgc
      721 gcaaagcctg ggcatttgtg atggtgctgg ggtggagccg gtatgcgtat gtggagtttg
      781 ttttcaacca aaaagcggaa acctggctgc gctgccaccg gaatgccctg gaattctttg
      841 gcggagtgcc gaagcggctg gtaattgaca acctcaaaag tgggatcacc caggcaattt
      901 gggatgatcc agtagtacag atggcctacc gggagtgtgc ggagcactat ggatttctga
      961 tcctgccctg ccgcccggtc acacccgaac acaaaggcaa ggtggaaggt ggtgtcggct
     1021 atgtgcaggg ccggttcatg gggggccgtg ggccgatgag cctgacccag gccaaccagg
     1081 aagtgcgcaa gtggtgtcaa ggggaagctg ggatgcgtat tcacggcacg accaaagaac
     1141 agccgcgccg ccagttcgag acggtagaaa aagggcatct aaaacccctg ccaacgaccc
     1201 cgtatgatct ggcaatctgg aaaaaggcca aactgcatcg cgattgccat gtggtttttg
     1261 agggttccta ctactcggcg ccgttccggc tggtggggca acacctgtgg gtgtgcgctg
     1321 ggacgcaaca ggtgaggctg ttcaacgcca aatacgaact ggtggccacg catgaacggg
     1381 cagccaaacc aggaactcgc cggacacacc gtgaccacta cccgccggag aaactaccgg
     1441 gcctggagcg cacgcgcgaa ggttgtcttg agcaggctgc cgcacttggc ccggcaatcc
     1501 gccaaactgt tgagcaaatc ctggctgacc cggtgctgga tcgtatccac caggcgggca
     1561 ggctgctgcg tctgagcgaa aaatatggct gtcaacggct gaatgatgcc tgccagcgag
     1621 cattggattt cggggatcca gcctacaaga cggtgaaagg tatcctcaaa caaaacctgg
     1681 atcaggaaga accaccgatc ccggtccagc tttccccggc gctgactttc tcacgccagc
     1741 cggatgagct ggttggggct ttggcggagg tgccgacatg gaactgaagc cgcagctttc
     1801 tcaccaactg cgccaattgc gcatgtccgg cgtgatggag accctggatg cccgcaaccg
     1861 ccaggcgatt gacggccagt ggtcgtacca ggaatttctc tcccgtctgt tggaagatga
     1921 gactgagcgc cgtgcccaga agcagctcag catgcgtctg cgccgcagca ccttgaatgc
     1981 cacaaaaacc attgaaggat ttgatttcca gttcaatccc aacctcaacc gccagcaaat
     2041 tctgcaacta gctgcctgcg aatatctgcg tgagcaccgc aacctgctga tctgcggccc
     2101 gaccggggtc gggaaaacgc atttagccca ggcgcttgga caagaagcgg cccgccaggg
     2161 gtttgatgtg cttttcgtga gcgtccagaa gatgctccag cacctcaacg gcggacgggc
     2221 ggatggttcc tgggaacgca ggctgggcaa ctacttgcgc ccggacttgc tcatcctgga
     2281 tgattttggc ctcaagccga tccagccgcc tggcccggaa gacctgtatg acgtgattaa
     2341 cgagcgctac gagcgcggca gcatcctgct caccagcaac cgagcaccca atgagtggcc
     2401 cgatctgttc ctcgatccgc tcctggcctc agcgggcctt gcccgcctcc ttgaccgtgc
     2461 cgaagtggtg attatccggg gcgccagtta ccgtgctcag ggccgcgctc gcctgactga
     2521 ggaggtgcca acagcccaga aacaacctta acaaaatacc cgctttacaa ccagactcgt
     2581 cgcgaaagtg gcgcggtgtt gccgaagatt actggtgcag tgtgggtgaa tatgggtgga
     2641 gcatttacgt gtgtctattg acaaccttga aggcgatggc tttcagcgcc tgcagctctg
     2701 ccaggcgctc ggcagcccac acattctgcc cggcaaacag gtattg
//
LOCUS       DAOOWR010000223         2744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_36936_length_2744_cov_3.835627, whole genome shotgun sequence.
ACCESSION   DAOOWR010000223 DAOOWR010000000
VERSION     DAOOWR010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36936_length_2744_cov_3.835627"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLB85_05780"
     CDS             complement(<1..643)
                     /locus_tag="PLB85_05780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU72874.1"
                     /translation="MIKVAINGFGRIGRLVLRAIYKYHPEMKVVAINDLTDAKTLAYL
                     LKYDSVHKIFSSEVKHTDDSIIVDGNAIRVFAEKDPEALPWKELGVEYVIESTGVFNT
                     KEAASKHLKAGAAKVILTAPAKDEVDATVVMGVNHTSLKPEHAVVSNASCTTNCLAPV
                     VKVLQDRFGIAGGLMTTVHSYTNDQRILDLPHKDLRRARAASMSMIPTSTGAAK"
     gene            complement(654..1628)
                     /locus_tag="PLB85_05785"
     CDS             complement(654..1628)
                     /locus_tag="PLB85_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOU72875.1"
                     /translation="MFLTGKQLGEVFLKAKKQRFGIIASNVVFDTQIRALVQGYAAVN
                     SDGLMQMSSGACKYAAGESQDIKVGAALISTMIKTFASQFPKSGVGLHIDHATPNYFD
                     FIVHCIEQNLVTSVMIDASKEDLEENIRITKEVVDIAHKHGVLVEGEIGHIKGAEDEI
                     VSDIELYTRPEEALEFVQKTNVDLFAASVGTNHGVSKGENIVLRLDLIKEIDDLLIQH
                     GVERGLVLHGASGLTDEQQRVAIANGVVKINKDTHYQMDMAEAIQAYWEKEKYAIVCP
                     PDVSPEDYIPNKGKFDPRKWLIKGEEKMKNTVMEFCRVTGSADNSILL"
     gene            complement(1690..>2744)
                     /locus_tag="PLB85_05790"
     CDS             complement(1690..>2744)
                     /locus_tag="PLB85_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOU72876.1"
                     /translation="LARKLQDLVNTQQQQFEALKLALEGRLEQIRANNETKLEEMRKT
                     VDEKLHDTLEKRLGESFKQVSERLELVHKGLGEMQNLASGVGDLKKVLTNVKSRGMMG
                     EFQLEAILEQVLTPEQYTRNFKPHKRRDEVVEFAIRLPGRDEDQESVYLPIDAKFPIE
                     DYSRLVEAWDAGDPGATETARKALYNRILGCARDIRDKYLNPPLTTDFALLFLPVEGL
                     YAEVLRDPALFEQLRRQYQVVVVGPTTVAAILNSLQLGFRTLAIEKRSGEVWKILSAV
                     KNEFGKFGKALEHTQKKLQEASNSIDTASHRSRQIQKKLNKVQELPVDETAQVLELED
                     DTGISAGTGAEEEDLA"
BASE COUNT          497 a          750 c          772 g          725 t
ORIGIN      
        1 ctttcgcggc gccggtggaa gtgggtatca tgctcatgga ggcggcgcgg gcacggcgta
       61 agtccttgtg gggcaggtca aggatgcgct ggtcattggt gtaggagtgc accgtggtca
      121 tcagcccgcc ggctatgccg aacctatcct gcagaacctt gaccacaggg gccaggcagt
      181 tcgttgtgca ggaagcattt gaaaccacag cgtgttcagg cttcagggaa gtgtggttca
      241 cacccatcac gacggtggcg tccacctcgt ccttggcagg agcggtgagg ataaccttgg
      301 ctgcgccggc tttgaggtgt ttggaagcgg cttctttggt gttgaaaacg ccggtggatt
      361 caatcacata ttccacgccc agctctttcc agggcagggc ttcggggtct ttttcagcga
      421 aaacccggat ggcgtttccg tccacgatga tgctgtcgtc ggtatgtttc acttcgctgg
      481 agaagatctt gtgcacgctg tcgtatttca gcaagtatgc cagggtcttg gcgtcggtga
      541 ggtcattgat ggccaccacc ttcatttcgg ggtggtattt atagatggct cggagcacga
      601 ggcgtccgat gcggccaaat ccattgatgg ctacttttat cattcacact cctttagagt
      661 aatatgctat tatcggcgct gccagtgacg cggcagaact ccatgactgt gtttttcatc
      721 ttttcctcgc ctttgatcag ccacttgcgg ggatcaaatt tgcccttgtt cgggatgtag
      781 tcttccggcg acacgtcagg tgggcagacg atggcgtatt tttccttttc ccaataggct
      841 tggatcgctt ccgccatgtc catttggtag tgggtgtcct tgtttatttt caccacaccg
      901 ttggcaatgg ccacgcgctg ctgctcgtcg gtgagtccag aagcgccgtg cagtaccagg
      961 cctcgttcca ccccgtgctg gatcagcagg tcgtcgatct ccttgatcag gtccaggcgc
     1021 aacacgatgt tttcgccttt ggaaacgccg tggttggtgc ccacggaggc agcaaaaaga
     1081 tccacgttgg ttttctgtac gaattccagc gcctcttccg gccgggtgta aagctcaata
     1141 tctgaaacaa tctcgtcttc ggctcccttg atgtggccga tttcgccttc caccaaaacc
     1201 ccgtgtttgt gggctatatc caccacttct ttggtgatgc ggatgttttc ttccaagtct
     1261 tctttcgaag cgtcgatcat cacggatgtc accaggtttt gttcgatgca gtggacgatg
     1321 aaatcaaaat agttgggggt ggcgtggtcg atatgcagac ccacaccgct tttggggaat
     1381 tgcgaggcaa aggttttgat catggtggag atcagcgccg cgccaacctt gatgtcctgc
     1441 gactcgccgg cggcatattt gcaggcgccg ctgctcatct gcatcaggcc gtctgaattc
     1501 accgccgcgt agccctgcac aagggcgcgt atctgggtgt cgaacacaac gttcgaggcg
     1561 atgatgccga aacgctgttt tttggctttg agaaatactt ctcctagttg cttgccagtt
     1621 agaaacattt ataacctccc tggtcagttg ttttattgct gtcatggttt tttggtttgg
     1681 gagtggctgt caagctaaat cctcctcttc cgctcccgta ccggcagata tgccggtatc
     1741 atcctccaat tcaaggactt gggcagtctc atcaacgggc agttcctgca ctttgttcag
     1801 ctttttctgg atctggcggc tgcggtggct ggccgtatcg atagaattgg aggcttcctg
     1861 cagctttttc tgggtgtgtt ccagcgcttt tccgaatttg ccgaattcgt tctttacagc
     1921 actgaggatc ttccagacct cgccagagcg tttttcgatg gccagggtgc ggaaaccgag
     1981 ctggaggctg ttcaggatgg ccgccaccgt ggttgggccc acaacaacaa cctgatactg
     2041 gcggcgtagc tgctcaaaga gggccggatc acgcaaaacc tcggcgtaga gcccctccac
     2101 gggcagaaat agcagcgcga aatcggtggt caggggcgga ttcagatatt tgtcgcggat
     2161 gtccctggca cagcccaaaa tgcggttgta gagcgctttg cgagcggttt ctgtggcccc
     2221 ggggtcgccg gcatcccagg cttccaccag gcgggaataa tcctcgatgg gaaacttggc
     2281 gtcgattggc aggtaaacgc tttcctggtc ttcatcccgg cccggcaggc ggatggcgaa
     2341 ttccaccacc tcgtcgcgcc gcttgtgggg cttgaagttg cgcgtgtatt gctccggcgt
     2401 gagcacctgc tccagaatcg cttccaactg gaactcgccc atcatgccgc ggctcttcac
     2461 gttggtgagc acttttttaa ggtcacccac cccgctagcc aggttctgca tttcacccaa
     2521 acctttgtgc accagctcca gccgctcgct cacctgcttg aaggattcgc ccaaccgctt
     2581 ttccagagtg tcgtgcagct tctcgtccac cgttttgcgc atttcttcca gcttggtttc
     2641 gttgttggcg cgaatctgtt ccaaccgccc ttccagagcc aacttcaagg cttcgaactg
     2701 ctgctgctgg gtgttcacca agtcctgcag tttgcgggcc aggg
//
LOCUS       DAOOWR010000224         2732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_37154_length_2732_cov_3.403810, whole genome shotgun sequence.
ACCESSION   DAOOWR010000224 DAOOWR010000000
VERSION     DAOOWR010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37154_length_2732_cov_3.403810"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..395)
                     /locus_tag="PLB85_05795"
     CDS             complement(108..395)
                     /locus_tag="PLB85_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU72877.1"
                     /translation="MAFSPVSSLLKQVLTQIGGDKYQPFILLYQGWKDVVGELLASRS
                     HPFRFHDSVLYVAVQNNSWLQELYLRKAEILRQCRAMIPQEIRDIIFLIRS"
     gene            complement(445..1182)
                     /locus_tag="PLB85_05800"
     CDS             complement(445..1182)
                     /locus_tag="PLB85_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72878.1"
                     /translation="MKNTLFNITRVLAVVVMIGALSLIGAGNPKVLPFSVLFFVAVFA
                     IVYLLNSYAKKNEREGKAGAGAKPIVQLIVVILFVAIVSVLGYLRHGILGYLVMFAFI
                     AAVFGLIYLAIKNRQRHFELTSSNPMTKKVLTAVTGILAIALPVIMVLASKLFKPDAI
                     SVIVTIIATLVFIALNVLALILINRKGSLSASVIGYILVILAAILPGIMVLMVTNDSG
                     AFAKTYLAALVAAVLAYFSLTQFEKVD"
     gene            complement(1197..1763)
                     /gene="rplI"
                     /locus_tag="PLB85_05805"
     CDS             complement(1197..1763)
                     /gene="rplI"
                     /locus_tag="PLB85_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU72879.1"
                     /translation="MKVILLNHIEKLGKKGEVVSVKRGFARNYLIPRKLAIYATPQNM
                     KHLSAIQNQAAEEEEKLIEELKKLDAKIRTLSLVFVRKVDEHDSMFGSVSEIDIVNEL
                     AAQDVNIHKSAVLMEKNIKSLGETVVQIRLHKDIVSELKVLVEKEARDEAVEEQVAAP
                     VEPEIPAEPDAVAEVVETEEPKPTEDEI"
     gene            complement(1760..2566)
                     /locus_tag="PLB85_05810"
     CDS             complement(1760..2566)
                     /locus_tag="PLB85_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72880.1"
                     /translation="MATISPFMGLLLMLVSLRSLVLRSGGKILSRALLWLVFPVLLGS
                     FTLRQPGSLLIVSDAVFGAGLVSLLYLYSLTRQGKPAVALVHGAILVIVYGALRFFLF
                     RTQLIAAHDLALAEMNKLVPQMMQLAKTQNTLAITRLLLPASWTVTQLLALTAGHLAF
                     LHLSGVRSILREFSLPAWYNLLILAVLPLYFIPPLREVLVNTLLALCFLPLVQGIGVI
                     LNWFSRLVSNPLVTLLVIALALLNLILVALLGFADTWLDFRKIRIKGTYA"
     gene            complement(2596..>2732)
                     /gene="rpsR"
                     /locus_tag="PLB85_05815"
     CDS             complement(2596..>2732)
                     /gene="rpsR"
                     /locus_tag="PLB85_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU72881.1"
                     /translation="RQFISDVGKIDPARMTGTCAKHQRKLTTEIKRARQMALLPFVIE
                     "
BASE COUNT          696 a          702 c          722 g          612 t
ORIGIN      
        1 tatatcaaga tgaagcatat cagcgctgct caccgcggcc aactccctct ccagattgcc
       61 aaaatcacag gataataccg aggccgcgat tcgcacttcc gccattatca actcctgatt
      121 agaaaaataa tgtccctgat ctcctggggt atcattgccc tgcattggcg caggatttcc
      181 gctttgcgca gataaagttc ctgcagccag gaattgtttt gcacagccac gtagaggact
      241 gaatcgtgaa aacggaaggg gtgggaacgt gaggccagga gttcgcccac aacgtccttc
      301 cagccctggt aaagcagaat gaaaggctgg tatttatcac cgccgatctg ggtcaatacc
      361 tgctttagca agctggaaac agggctgaag gccacgtttt accaccttcc ttggggtaaa
      421 aagaccgatc cggccgaggc cggatcagtc aactttttca aactgggtaa gcgagaaata
      481 tgccaggacc gcggctacca aagccgccag ataggtcttg gcaaaagctc cggaatcgtt
      541 tgtgaccata agcaccatga tcccggggag aattgccgcc aggatgacca gaatgtaccc
      601 aatcacagag gcagacagac tgcccttgcg gttgatcaga atcagggcca gcacattcag
      661 ggcgatgaac accagggtgg ctataatggt aacgatcaca gaaatggcgt caggcttgaa
      721 gagcttgctg gcgagcacca taatgacagg cagggcgatt gccagaatgc cggtgaccgc
      781 agtcagaacc tttttcgtca taggattgga cgaggtgagc tcaaagtggc gctgtcggtt
      841 tttgatcgcc agatatataa gcccaaaaac ggctgcaata aaggcgaaca taaccaaata
      901 gcccaggatg ccgtgacgca gataacccaa aacggagact attgcgacaa agaggatcac
      961 cacgatcaat tgcacaatcg gtttggctcc cgccccggct tttccctccc gctcgttttt
     1021 cttggcgtag ctatttagca ggtaaactat cgcgaacacc gcgacaaaga aaagaacgga
     1081 aaagggtagc actttggggt tgccggctcc gatcagggat aaagctccga tcatcacgac
     1141 caccgccagg accctggtga tgttaaacag agtgtttttc atgggtttct cccagtttat
     1201 atttcgtctt cagttggttt cggttcttct gtttcgacca cttcagcaac agcatcgggt
     1261 tcagccggga tttcaggttc caccggagcg gcaacttgtt cttccacagc ttcatccctg
     1321 gcttctttct cgaccagaac tttcagttcc gacacgatat ccttgtgcag acgaatctgg
     1381 acaacagttt cgcccaggct cttgatgttc ttttccatca ggaccgcgga tttgtgaata
     1441 ttaacatcct gcgcggccag ttcattcaca atgtctatct cagacacgga gccgaacatg
     1501 ctgtcatgtt cgtccacttt acggacaaaa accaaactaa gggttctaat cttggcgtca
     1561 agctttttca gttcctcgat cagcttttct tcttcttccg cggcctgatt ctggatggca
     1621 ctgaggtgtt tcatgttctg gggagtggca tagatggcca gcttgcgggg aattaggtag
     1681 tttctggcga atcctctttt tacggagacg acttcacctt tcttgcccaa cttttctatg
     1741 tgattcaaga ggatgacttt catgcgtatg ttcctttaat tcttattttc ctgaagtcga
     1801 gccaggtatc ggcaaatccc aaaagggcga ccaggatcag gtttaataat gccagcgcga
     1861 tcaccagcaa agtgacaaga ggattggaga cgaggcgtga aaaccagttc agaatcacgc
     1921 caatgccctg cacaagaggc aaaaagcaca atgcgagcag agtgtttacc aatacttcgc
     1981 gcagcggagg gatgaaatac agcggcaaaa ctgccaggat caagagatta taccaggcag
     2041 gtaggctgaa ttccctaagg attgatctta ccccgctcag atgcagaaag gccagatgcc
     2101 cggctgtgag agccagtagc tgagtgacgg tccaactggc cggcagcagc aaacgggtga
     2161 tggccagggt gttttgggtt ttcgccagtt gcatcatctg gggaacgagt ttgttcattt
     2221 cggccaaagc caagtcgtgc gccgcaatga gctgggttct aaacaggaag aacctcagcg
     2281 cgccatagac gatgaccagt atcgctccgt ggaccagcgc caccgccggt tttccctgcc
     2341 gggtgaggct gtagagatac agcagggaaa ccaatccggc accgaacact gcatcggaaa
     2401 cgatcagcaa ggagcccggt tgccgcaggg taaaagagcc cagcaggacc gggaaaacca
     2461 accagagcaa agccctggat aagattttgc ccccgctccg cagcaccagg ctacggaggc
     2521 tgaccagcat cagaagcagg cccatgaagg gactgatggt tgccatggca cccaggagca
     2581 aggcgataat gggcactatt cgatcacgaa tggaagcagc gccatttggc gggcgcgctt
     2641 gatttcggtg gtcagcttcc tttggtgctt ggcacaggtt ccggtcatgc gggcgggatc
     2701 tatcttgccc acatcggaga tgaattggcg ca
//
LOCUS       DAOOWR010000225         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_37307_length_2723_cov_2.758996, whole genome shotgun sequence.
ACCESSION   DAOOWR010000225 DAOOWR010000000
VERSION     DAOOWR010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37307_length_2723_cov_2.758996"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="PLB85_05820"
     CDS             complement(<1..1507)
                     /locus_tag="PLB85_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU72882.1"
                     /translation="MLKDTRREIRPNEYDSVRIKLASPETIINEWSHGEITKPDTLNY
                     RTLKPEKDGLFCERIFGPERDYECACGKYKKKRFQNTVCDRCNVLVTTSRVRRTRMGH
                     IALAVPIAHIWFVKSAPSKIGTLLDMTVKDLERILYYESFIVIDPGDSPYEKYELIEV
                     DEYYEIRDKVGDNFIAMMGAEAIRELLARIDLHNEALNIRSRLKMEKATLHKQKLINK
                     LKIVDAFIKSGNQPEDMIMQALPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNR
                     LKQLLDIRAPEVILRNEKRMLQEAVDALIDNSRKPRPVRGRGNRPLKSLTDQLKGKQG
                     RFRQNLLGKRVDYSGRSVITVGPELKLYQCGLPKDMAVELFKPYLIERLQKMGEVDKV
                     KNAKKLIEKKQPEIWSILEEVIRDYPVLLNRAPTLHRLGIQAFMPVLTDNKAIQLHPM
                     VCVPFNADFDGDQMGVYVPLSHEAQIEARVLMLSTRNLLLPSNGRLAMAANQ"
     gene            complement(1510..>2723)
                     /gene="rpoB"
                     /locus_tag="PLB85_05825"
     CDS             complement(1510..>2723)
                     /gene="rpoB"
                     /locus_tag="PLB85_05825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU72883.1"
                     /translation="FTNSSLKAKPGMEGVVIDVKMFSRLEEGMEQEEDKDEKYNKLKS
                     DLNLRRRKVEEFKEEKLSAALLGQVAQTIWDEKTNVYYIAPGKKITKADIARINFKKL
                     DLDAHLVQDTEKNEQIYSDIILKIKQSLEQSENIYRKSRERIKHGDELQYGVRKMVKV
                     YVAKKRKIEVGDKMAGRHGNKGVISIVAPIEDMPFLEDGTPVDIVLNPLGVPSRMNIG
                     QIMETHLGMAAQTLGFEVETPIFDGASVEEIESALQEAGLPTDGKQVLYDGKNGEPFK
                     ERVTVGIIYMMKLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEA
                     YGAAHLLEEMLTIKSDDVDGRNNAFKAITRGENPPPPGIPESFNVLISELKSLGFDIE
                     FLKDNEKGEVR"
BASE COUNT          520 a          704 c          723 g          776 t
ORIGIN      
        1 cctggttggc agccatagcc agcctgccgt ttgaaggcag gagaaggttt cgggtggaaa
       61 gcatcagcac cctggcctcg atctgcgcct cgtgcgaaag aggcacataa acacccatct
      121 ggtcaccatc gaagtcagca ttgaagggaa cgcagaccat gggatgcagt tgaatggcct
      181 tgttgtctgt aagtacgggc atgaaagcct ggatacccaa gcggtgcagg gtgggcgcgc
      241 ggttcagcag tacaggatag tcacgtatca cttcttccag gatggaccag atttccggct
      301 gtttcttctc gatgagcttc ttggcgttct taaccttgtc cacttcgccc attttttgca
      361 ggcgctcgat cagataaggt ttgaagagtt ccactgccat gtctttggga agtccgcact
      421 ggtagagctt cagttctggg ccgactgtga tcacagaacg gcctgaatag tcaacgcgtt
      481 tgcccagcaa attctgacgg aaacggccct gttttccttt taactggtca gtgagggatt
      541 tcaggggccg gttgcctctg cccctcactg ggcgcggttt gcgggaattg tcgatcaggg
      601 cgtccaccgc ttcctgaagc atgcgttttt cgtttcgcag gatgacctcg ggggcacgga
      661 tgtccagaag ctgtttaagc cggttattgc gcgtgatcac gcgtcggtag aggtcattga
      721 aatccgcggt ggcaaagcgt ccaccttcca ggggcaccaa tggcctcagg gtaggcggca
      781 atactggcaa agcctgcatt atcatgtctt cgggctgatt accggatttg atgaaagcgt
      841 ccacgatctt gagcttgttg atcagctttt gcttgtgcag ggttgccttc tccattttca
      901 gccgggagcg gatgttcagg gcttcattat gcaggtcgat ccgggcaaga agttcccgaa
      961 tggcctcggc gcccatcatt gcgatgaagt tgtcccccac cttgtccctg atctcatagt
     1021 attcgtcaac ttcgatcagt tcgtactttt cgtaggggct gtcacccggg tcgataacta
     1081 tgaaggattc gtaataaagg atgcgttcca agtctttgac cgtcatgtcc agcagtgtgc
     1141 caatcttgct gggcgcactt ttcacgaacc agatgtgggc aattggcaca gccaacgcga
     1201 tgtggcccat gcgggtacgg cgcacgcgtg atgtggtcac cagcacgttg cagcggtcgc
     1261 agaccgtgtt ttggaagcgt tttttcttgt atttgccgca ggcgcattcg taatcccttt
     1321 ctgggccgaa gatcctttcg cagaaaaggc cgtccttctc gggtttaagg gtgcggtagt
     1381 tgagagtgtc tggttttgtt atctctccgt gcgaccattc gtttataatc gtttccggtg
     1441 atgctagctt gatgcgcaca gaatcgtatt cgttggggcg gatttcacgt cgggtgtctt
     1501 ttaacatctt tacctcactt cgcccttttc attgtcttta aggaattcga tatcgaatcc
     1561 cagtgacttg agctcgctga tgagcacgtt gaatgattcc ggaatgcctg gagggggtgg
     1621 gttctcacct cttgtgatgg ctttgaaggc gttattgcgc ccatctacat catcgctctt
     1681 aatggtgagc atttcttcca gcagatgcgc ggctccgtat gcttccagag cccaaacttc
     1741 catttctccc aaacgctgtc cgccgtgctg ggctttccca cccagaggct gctgcgtgat
     1801 cagggagtaa ggtccggtag agcgagcgtg catcttatcc gccaccaagt ggttgagttt
     1861 catcatataa atgataccca ctgttacacg ttccttgaaa ggttcaccgt ttttcccgtc
     1921 gtagaggacc tgttttccgt cggtgggcaa tccagcctct tgcagcgcgg attctatttc
     1981 ttccactgaa gcgccgtcga agatcggggt ttccacctca aagcccaaag tctgcgcggc
     2041 catgcccaga tgggtttcca tgatttggcc gatgttcatg cggctgggaa cgcccagagg
     2101 gttaagaacg atatccactg gtgttccatc ttccagaaac ggcatatctt cgattggggc
     2161 cacgatggag atcacgccct tgtttccatg gcgtcctgcc atcttgtccc caacctcgat
     2221 tttgcgtttc ttggccacgt agaccttcac catcttgcga acaccgtatt gcagttcgtc
     2281 accgtgtttg atgcgttcgc gggacttgcg ataaatgttc tcgctctgtt ccagtgattg
     2341 cttgattttg agaatgatgt cgctgtatat ctgttcgttc ttctcggtat cctgaaccag
     2401 atgggcgtcc aagtccagtt ttttaaaatt gatccgggct atatccgcct tggtgatctt
     2461 tttgccaggg gctatataat agacatttgt cttttcatcc cagatggttt gggcaacttg
     2521 gcccagaagg gctgcggaaa gcttttcttc cttgaattcc tccacctttc tgcggcgcag
     2581 gttcaggtcg cttttcagct tgttgtattt ttcgtccttg tcctcttcct gttccatgcc
     2641 ctcttccagc cgggaaaaca ttttcacgtc aatcaccacg ccttccatgc cgggtttagc
     2701 cttgagcgag ctgttggtga aat
//
LOCUS       DAOOWR010000226         2722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_37319_length_2722_cov_3.735283, whole genome shotgun sequence.
ACCESSION   DAOOWR010000226 DAOOWR010000000
VERSION     DAOOWR010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37319_length_2722_cov_3.735283"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..121
                     /locus_tag="PLB85_05830"
     CDS             11..121
                     /locus_tag="PLB85_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HOU72884.1"
                     /translation="MYITDTLWPDFDKAAFIQAMINFQGRKRRFGGVKNL"
     gene            118..915
                     /locus_tag="PLB85_05835"
     CDS             118..915
                     /locus_tag="PLB85_05835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOU72885.1"
                     /translation="MTALQKRIAVSVVFIPLILAALWFGGPWLIIFFALAVLIGSGEY
                     ISMLRRAKYPVPWLWIAASLASYAQIVLLPGFELVTVWVLLLLAFLEALLRWDAQKSV
                     LQAALELFGVLYAAFLPALCVRLGLEHQESRLLLGLVVLIWCADSLAYFVGMKWGKRR
                     GIVKISPNKSLEGFLAGALAPFVIVTILYIGKLYGDLTLLLLTAFAAGIVGQLGDLAE
                     STLKRFCQVKDSSNLIPGHGGILDRSDSILMAGSFLYCATQIIQQVR"
     gene            921..2648
                     /locus_tag="PLB85_05840"
     CDS             921..2648
                     /locus_tag="PLB85_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU72886.1"
                     /translation="MIKRTLLPLLMLGLISLMFAQAADLFISEYVEGSSNNKAIEIFN
                     GTGAAVDLSNYSMKLASNGSPTWSTTNSVTLSGTLANNDVFVIANAQANPTILGVADM
                     THTVTYFNGNDCLGLFHGDTLIDIIGVLGTDPGTAWPVAGTEGATLNHTLIRKPEVDS
                     GNTDWIAGAGTNMDNSEWIVHPQDYVDDLGSHTFDPGGSEHAATPTFNPPAGVYAQPI
                     SVTISSTTPGATIRYTTDGSNPTETSTQYTSPISLDTNTTLKAIAFASGFDPSYVATA
                     SYIFPQVVQNMTQLRNQTVGDGTVYMVAGEVILTFKQNFRNQKYVQDNEAGVLIDDTA
                     GMITTNYNLLDGITGLTGTIAFYNNMLQLTPVADPGPATSTNNTIVPPTVTIAQINAN
                     VASYQARLVRIANAHFVESGTFETGHNYTLEDDTGTIVFRTTFYDADYIGEPIPVGNF
                     NVRVLVNQFNQTPQVTARALSDWSGVPNEDNVATPNRLLGNYPNPFNPSTTISFTTAK
                     AAPVQITIYNLKGQAVRTWNLETEAGGNHSVQWDGLDDNGLSLSSGVYFYRMFSGAYS
                     STRKMVLMK"
BASE COUNT          606 a          864 c          657 g          595 t
ORIGIN      
        1 gtattcggaa atctacatca cagacaccct ctggccggat tttgacaaag ccgccttcat
       61 ccaggccatg atcaatttcc agggccgaaa acgccgcttt ggaggggtta agaacctatg
      121 accgcgctgc aaaagagaat cgctgtttcc gtggtcttca tccctctgat cctggccgcc
      181 ctgtggttcg gcggcccctg gctgataatc tttttcgccc tggcggtgct gattggttca
      241 ggcgaataca tctccatgct gcgccgggcc aagtatccgg tgccctggct ctggatagcc
      301 gcctcgctgg cctcatacgc ccagatagtt ttgttgcctg gctttgagct ggtcaccgtc
      361 tgggttctgc tgctgctggc ttttctggag gccctgctgc gctgggacgc ccaaaagtct
      421 gtcctccagg ccgctctgga actcttcggg gtactctacg ccgcctttct gcccgctctc
      481 tgcgtgaggc tggggctgga acatcaggaa tcccgcctcc ttttgggact ggtggtccta
      541 atctggtgtg ctgacagcct tgcctacttt gtgggtatga aatgggggaa gaggcgcgga
      601 atagttaaga taagcccaaa caagtctctg gagggtttcc ttgctggagc gcttgcccct
      661 tttgttatag tgactatatt atatattgga aaattgtatg gcgatttgac gcttttgctg
      721 ctgacggctt tcgctgccgg aatcgtcggc cagcttggcg atttggcgga aagcacgttg
      781 aagcgttttt gccaggttaa agacagctcg aacctgatac ccggccacgg tggaattctg
      841 gatcgcagcg acagcattct gatggccgga tcctttttat actgtgcaac tcagattata
      901 caacaagtga ggtaaacaac atgatcaaaa gaacactact gccccttctg atgctgggat
      961 taatctccct gatgttcgcc caagcggctg acctttttat ctctgaatat gtggagggaa
     1021 gctcaaacaa caaagccatc gagatcttca acggcaccgg agccgccgtc gatctgtcca
     1081 attattccat gaagctggcc tccaacggtt ccccgacctg gtccaccacc aacagcgtga
     1141 ccctttccgg aaccctggct aacaatgatg tattcgtgat cgccaatgcc caagcaaacc
     1201 ccaccatcct tggtgtcgcg gacatgaccc acaccgtcac ctatttcaac ggcaatgact
     1261 gcctgggcct cttccacggt gataccctca ttgacatcat cggcgttttg ggaaccgatc
     1321 ccggcaccgc ctggccagta gctggaaccg agggcgcaac cctcaaccac accctcatcc
     1381 gcaaaccgga agtggattcc ggaaacaccg actggatcgc gggagcgggc acgaatatgg
     1441 acaactctga atggatcgtc catcctcaag attacgtaga cgacctcggc tcccatacat
     1501 ttgatccagg tggttccgag catgccgcca cacctacctt caatcctcct gccggcgttt
     1561 atgcccagcc catctctgtg accatctctt caaccactcc cggtgccacc atccgctaca
     1621 ccaccgacgg cagcaaccca accgaaacct ccacccaata caccagcccc atttcgcttg
     1681 atacgaatac caccctgaaa gccattgctt ttgccagcgg tttcgacccc agctatgtgg
     1741 ccaccgcgtc ctacattttc ccccaggtgg tgcagaacat gacccaactg agaaaccaga
     1801 ccgttggtga cggaaccgtt tacatggtgg ccggcgaagt gatcctcacc ttcaagcaga
     1861 acttccgcaa ccagaaatac gtgcaggaca atgaagccgg ggtgctcata gacgacacag
     1921 ccggaatgat aaccacgaac tataacctcc tggacggcat caccgggctc accggaacca
     1981 tagccttcta caacaacatg ctccagttga ctccagtggc cgatccgggc cccgccacct
     2041 ccaccaacaa caccatagtc ccgcctacgg tgaccatcgc ccagatcaac gccaacgtcg
     2101 cttcctatca ggcccggttg gtcaggattg ccaacgccca tttcgtggaa tccggcacct
     2161 ttgaaaccgg ccataactac actctggaag acgacaccgg caccatagtc ttccgcacca
     2221 ccttctatga tgcggattac attggcgaac ccatccccgt cggcaatttc aacgtccgtg
     2281 tgctcgtgaa ccagttcaac cagacaccgc aggtgaccgc ccgcgccctt tctgactgga
     2341 gtggagtgcc aaacgaagac aacgtcgcca cccccaaccg gctgctgggc aactatccca
     2401 accccttcaa tcccagcacc accatctcct tcaccactgc caaggccgct ccggttcaga
     2461 tcaccatcta caatctcaag ggccaggctg tcaggacctg gaacctggaa actgaagccg
     2521 gcggaaacca cagcgtccag tgggacggcc ttgacgacaa cggactttcc ctgagcagcg
     2581 gagtctattt ctaccgcatg ttctccggcg cctattcatc cacccgcaag atggtgctga
     2641 tgaaataatc acctgccctg acgtccgccc ggactggatt ccagcgggct tgcgagcatg
     2701 gatcgcgagt gaagctggcc at
//
LOCUS       DAOOWR010000227         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_37936_length_2689_cov_3.940395, whole genome shotgun sequence.
ACCESSION   DAOOWR010000227 DAOOWR010000000
VERSION     DAOOWR010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37936_length_2689_cov_3.940395"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PLB85_05845"
     CDS             <1..1374
                     /locus_tag="PLB85_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72887.1"
                     /translation="LSVFNRDGSPGDAIRAITWWINQNMSFTSNNERPHQPVRIFAKR
                     FGRCGEYADFSSAVARTALIPCTNISSVSTDHTWNEFWEDGWVSWEPVNGYLNNPLVY
                     ENGWGRVFGSVFEERSDGLFSPVTERYSEGVATINIQVVDQDMQPVDAARVVLAIFES
                     SPRFDCEAYTDNNGMVSFKVGENRDYRARTETMFGQYPAIPGTYTQLVANTVNGETYN
                     YQFQIAAPMPQPTIEALATPPDPEQDYRFTVDFQSLGYFVTGRTLWDDIDVLGAPARY
                     YQHVDDPSTVSFLVVDADNAIFLDLDNFCSAYSYTGPVASGNAVFDIPAGQNWYALLD
                     NSQRHGNAVLLSGTLAFEYWNTAVSDPQLPAAVFSLDAIAPNPFSEKTRVGLQLKEPA
                     EMGLSIYNLRGEKVREFGAQKLSSGQQWLEWDGRNDHAEALPSGVYFLKASAGKDVQV
                     RKLLLLK"
     gene            1554..2027
                     /gene="nuoE"
                     /locus_tag="PLB85_05850"
     CDS             1554..2027
                     /gene="nuoE"
                     /locus_tag="PLB85_05850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU72888.1"
                     /translation="MEQDFSRLDALLQEYEGRKGSLIPFLQAAQELEGYLSRPALKYI
                     SERMKVPAAEICGVVTFYSMFRLEPQGKHIIRVCKGTACYVSEADGIKDALIENLKLE
                     DGKLTTDDMLFTLMEVSCLGCCSLAPVIMIDDKTFGKLTPEAIPRILEKFRNGEA"
     gene            2070..>2689
                     /locus_tag="PLB85_05855"
     CDS             2070..>2689
                     /locus_tag="PLB85_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU72889.1"
                     /translation="MLNLDQIKKDYQEALAHYKKRIVVCAGTGCIANGSLKIYSALKN
                     SLAMHSLDVSIELKAHDEHDHDVLISGSGCQGFCQVGPLVTVEPDGILYAHVKPEDAD
                     EIVGQTIVQGKLIDRLLYVNPADGRHCKGAGDIPFYTRQKRTALKECGHLDPENVNEY
                     ISHGGYFAARDAYQKYSAEELCKLYQDSGLRGRGGGGFSTGLKWELT"
BASE COUNT          636 a          721 c          755 g          577 t
ORIGIN      
        1 ctgagcgtgt tcaaccgcga cggatcaccc ggagacgcca tccgcgccat cacctggtgg
       61 ataaaccaga acatgagttt cacctcaaac aacgagcgtc cgcatcagcc ggtgcgcatt
      121 ttcgccaaac gatttggacg ctgcggcgaa tacgccgact tcagttccgc cgtggcacgg
      181 accgccctta tcccctgcac caatatcagt tccgtctcca ccgaccacac ctggaacgag
      241 ttttgggaag acggctgggt ttcctgggag ccggtgaacg gatatctgaa caacccgctg
      301 gtgtatgaaa acggatgggg cagggtgttt ggcagcgtgt tcgaggaaag gtccgacggc
      361 ctgttctctc cggtcacgga gcggtattcg gaaggcgtgg ccaccatcaa cattcaggtg
      421 gtggatcaag acatgcagcc ggtggacgcc gcaagggtgg ttctggccat ctttgagtcc
      481 tcgccccgct tcgattgcga ggcctataca gacaacaacg gcatggtgag cttcaaagtg
      541 ggcgaaaacc gtgattaccg tgcccggacg gaaaccatgt ttgggcaata tcctgccatt
      601 cctggaacct acacgcaact ggtggcaaac acggtgaacg gcgaaaccta taattaccag
      661 ttccagatcg ccgcgccgat gccccagcca acgatcgagg cgttggccac accgcctgat
      721 ccggagcagg attaccgttt cacggtggat ttccagagcc tgggctattt tgttaccggc
      781 agaacgcttt gggacgacat cgatgtgctg ggcgcaccgg ccagatatta ccaacatgtg
      841 gatgatccct ccaccgtttc ctttttggtg gttgacgctg acaacgccat cttcctggat
      901 ttggacaatt tctgcagcgc ctactcttat acgggcccgg ttgcttccgg caacgcggtt
      961 tttgatattc ccgcgggcca gaactggtat gccctgctgg ataattcgca acggcacggc
     1021 aacgccgttt tgctctccgg aacgctggcc ttcgaatatt ggaacaccgc cgtcagcgat
     1081 ccgcaactgc cggctgcggt tttcagcctg gacgccatcg cgcccaatcc tttcagcgaa
     1141 aagaccaggg ttggcctgca actgaaagag ccggcggaga tggggttgag catttacaac
     1201 ctccggggag agaaggttcg cgagttcggc gctcagaaac tctcctcggg ccagcaatgg
     1261 cttgagtggg acggaaggaa tgaccacgcc gaagccttgc ccagcggggt atatttcctg
     1321 aaagcgtctg cgggaaagga tgtccaggtt cgcaaactgc tgctgctgaa ataagccgcc
     1381 tacagcctgg gtagtccaga accatctgtc tgacaggaag atggagcaag catctgagaa
     1441 ttgttccaca ttctatttga caaagaccgc ctctctgtaa atgtgttcat ttcgtgcccg
     1501 cggagagaca gtcccgctgg gcgtgacact gaaatcaaag tcaaggagtc gcgatggaac
     1561 aggacttcag ccggctggac gcgttgttgc aagaatacga aggcaggaag ggatcgctca
     1621 tacccttttt acaagccgcg caagaacttg agggatacct ctcccgaccg gcactcaaat
     1681 atatatctga acgaatgaaa gtgccggctg ccgaaatctg cggtgtggta accttctatt
     1741 caatgtttcg cctggaacca cagggcaaac acatcatccg ggtttgcaaa gggacagcct
     1801 gctacgtgtc agaggcggac gggatcaagg acgcgttgat cgaaaacctg aagctggagg
     1861 acggcaaatt gaccactgac gacatgctgt tcacgctgat ggaagtgtcc tgcctgggct
     1921 gctgctcgct ggcgccggtg atcatgatag acgacaagac ctttggcaaa ctcactccgg
     1981 aagccatacc cagaatcttg gaaaaattcc gcaacgggga agcctgatgg gagcgatcaa
     2041 gcttatagat gctttattag agatggataa tgcttaattt ggatcaaatc aaaaaggact
     2101 atcaggaagc ccttgctcac tacaagaaac gcatagttgt ctgcgccgga acaggttgta
     2161 tagccaatgg ttccctgaag atttacagtg ccctgaagaa cagccttgcc atgcactccc
     2221 tggatgtatc catcgagctg aaagcccatg atgaacacga tcacgatgtg ttgatctcgg
     2281 gaagcggctg ccagggcttt tgtcaggttg gtccgctggt cacggtcgag ccggatggca
     2341 tcctctatgc tcacgtgaaa cccgaagacg ccgatgagat cgtgggtcag accattgtgc
     2401 agggcaagct gatcgaccgt ttgctctatg tcaatcccgc tgatggcaga cactgcaagg
     2461 gcgcgggaga cattcccttt tacacccggc aaaagcgcac cgcgcttaaa gaatgcggtc
     2521 acctggaccc tgaaaacgtg aacgaataca tcagtcacgg tggttatttt gctgctcgtg
     2581 acgcctatca gaaatacagc gctgaagaac tctgcaaact ttatcaggat tctgggttgc
     2641 gtgggcgtgg cggtggtgga ttctccaccg gcctgaagtg ggaacttac
//
LOCUS       DAOOWR010000228         2686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_37987_length_2686_cov_3.383884, whole genome shotgun sequence.
ACCESSION   DAOOWR010000228 DAOOWR010000000
VERSION     DAOOWR010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37987_length_2686_cov_3.383884"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2580
                     /locus_tag="PLB85_05860"
     CDS             <1..2580
                     /locus_tag="PLB85_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72890.1"
                     /translation="DWQHDVYGDTPSDIPSFFIRVGEGKEAVVDGGSVLISHACNVPS
                     TISQDFSSQTSGEDTIHSGYGALNAVLCHEFGHSLGFVDLYNVHTWQPMVGVFDIMDS
                     GGSGILMDILDDGSYVLLEGALPGLPGAWSRELVFGDDLRSRGLLRDVEYVNLFREMP
                     LAASSHRQQGDKLIPQILRIPLSPIEYILVENRSVDPDGDGATALKASDDGRVILYPT
                     PIDDPENIPSYEYDYLLPSFLKEDGSSVGGGLLVWRVNEDVIYSQGVTGDDGEFLSNY
                     ANNTINTLYTKRGVEIIEADNLADIGNQWSWFWTGTQYEYFHAKKPLQDGSGHFVSWS
                     LEPWKPVLNGTTEPPLKDSWGMGSQYWLSGIGNPAAIMNLTVNSGWFDETSVTAYGIQ
                     GLLPGPFINSSYSNLDLPLIGPQSITLLSHYDGRWTDLMGPFAWDGETLDQPVISADQ
                     DGDGYKELILSHANTLEIVDFSQDTLQSKTINFPDPISTAPIAIRDTVFVSTATCLSS
                     VVDNMVKDMVQLPQILRLGSYGTDLVTLSQDLLHIIDTRDLYIKCEVELPEPFGDYEP
                     VTFTGAASGLRMLFMTANSGNIYKFQDNRLQMIFQNTQAEKPTQLGIAWYMDSSMQGA
                     VNPTIFWGCGNRIFAIKHDGTLLGGYPHNAFPLTFSAREHVYALISYPQVLLYLPVPQ
                     RGHVAWLADEGILWQRSLLTDDTVFGSQLAFGPINLESHNLFWYYTDADGRLFIHRKA
                     LGMLNNGIYWNGFRNGCDGSFEGHGLYDDIPPVQDFTAFVFPNPVRDSQFRVRIENFD
                     TTVELRLYDINGSLLQSHSLPANGILRRDVALDSGKLASGVYILLVRKGAENKRIKFA
                     VEK"
BASE COUNT          613 a          782 c          685 g          606 t
ORIGIN      
        1 gattggcagc atgatgtgta tggcgacact ccttccgaca tcccctcctt tttcatcagg
       61 gtgggcgaag gcaaggaagc cgtggtggat ggcggctctg tgctcatcag ccatgcctgt
      121 aacgttcctt caaccatttc gcaggatttt tccagccaaa cctcaggcga ggataccatc
      181 cacagcggct acggtgccct aaacgccgtg ctctgccatg aatttggaca ttccctcggc
      241 tttgtggacc tctacaacgt ccatacctgg cagccgatgg ttggggtttt cgatatcatg
      301 gacagcggcg gttctggcat cctgatggat attttggacg acggctctta tgtgcttctg
      361 gaaggcgctt tgccgggatt gcccggcgct tggtcccgcg aactggtttt cggagacgac
      421 ctccgctccc ggggcctgtt gagagacgtg gaatatgtta acctgttcag agaaatgccc
      481 ctcgccgcca gcagccaccg gcaacaggga gacaaactca tccctcagat cctgcgcatt
      541 cccctatccc ccatagaata catattggtc gaaaaccgca gcgttgatcc agacggcgat
      601 ggagccaccg cgctcaaggc cagtgacgac ggccgggtta tcctctaccc taccccgatt
      661 gacgatcccg aaaacatacc cagttatgaa tacgactatc tgctgccctc ttttctgaag
      721 gaagacggct cctccgtggg tggcggtctc ttggtctggc gtgtgaatga agatgttatc
      781 tactctcaag gcgtaaccgg cgatgacggg gagttcctct ccaattatgc caacaacacc
      841 ataaacacac tctacaccaa gcgtggcgtg gagatcatcg aagcggacaa tctggccgac
      901 attggcaacc aatggtcatg gttctggact ggaacccagt atgagtattt tcacgcgaaa
      961 aagcccttgc aggatggatc cggtcacttc gtgtcatgga gcctggagcc ctggaagcct
     1021 gttctgaacg gcaccacaga accacccctc aaagacagct ggggaatggg ttcgcagtat
     1081 tggctcagcg gaatcggcaa ccccgccgct atcatgaatc tcaccgttaa cagcggttgg
     1141 ttcgatgaaa cctcagtcac tgcctacggt attcaaggac tcctgcccgg gcccttcatc
     1201 aattccagct acagcaacct cgatctacct ctgatcgggc cccaatccat cactctgcta
     1261 agccactatg atggccggtg gaccgacctg atggggccat tcgcctggga cggtgaaacg
     1321 cttgaccagc ctgtgataag cgcggaccag gatggcgacg gctacaagga actcattctc
     1381 agccacgcca atactctgga aatcgtggat ttctcacagg acaccctgca aagcaaaacc
     1441 atcaactttc ccgatcccat cagcaccgcg cccatcgcaa tcagggacac ggtgttcgtg
     1501 agcaccgcca cctgcctaag ttccgtggtg gacaatatgg tgaaggacat ggtccagctt
     1561 ccccaaatcc tgcgcctggg cagctacgga accgacctgg tgacactcag ccaggacctt
     1621 ctgcacatta tcgacacccg agacctttac atcaagtgcg aggtggaact gccggaaccc
     1681 tttggcgatt atgaacctgt gactttcact ggagcggcga gcgggctcag aatgctcttc
     1741 atgacggcta acagcggcaa tatctacaag ttccaggata accgcctcca gatgattttc
     1801 caaaacacgc aggcggaaaa gcccactcaa ttgggaatag cctggtatat ggacagctca
     1861 atgcagggag cggtcaaccc cactatcttt tggggttgcg gcaaccgtat cttcgccatc
     1921 aaacatgacg gcaccctgct cggcggctat ccccacaatg ctttcccgct cactttttct
     1981 gcccgggaac atgtttacgc gctaatatcc tatccacaag tgctgctcta tctgcctgtg
     2041 cctcaaagag gccatgtggc ctggcttgca gatgaaggca tcctctggca gagatccctg
     2101 ttgacggacg atacagtttt cggctcacag ctcgcgtttg ggcccattaa cttggaatcc
     2161 cacaatctct tttggtatta tactgacgcc gatggcaggc tgttcattca ccgaaaagct
     2221 ttgggaatgc tgaacaacgg aatatactgg aatgggttcc gcaatggctg cgatggcagc
     2281 tttgaaggcc acggcctgta tgacgacatc ccgccagttc aggatttcac agcctttgtt
     2341 tttcccaacc ccgtgcgcga ttcccaattc agggtccgca tcgagaattt tgacactact
     2401 gtggaactgc gtctttatga catcaacggc tcgctgctgc aaagccactc tctgcccgcc
     2461 aacggcatcc tgcgccggga tgtggccctg gacagcggca aacttgcatc tggcgtttac
     2521 attctcttgg tgcgtaaagg cgctgagaac aagaggataa aattcgctgt ggagaaatga
     2581 cccaggaggc caatgtatga aaaaaaccat cttgctcata ttgggcctgg cgctgctaac
     2641 cggcgcctat gccgatggct gggccggatg ctgccttaac atccaa
//
LOCUS       DAOOWR010000229         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_38041_length_2683_cov_3.191781, whole genome shotgun sequence.
ACCESSION   DAOOWR010000229 DAOOWR010000000
VERSION     DAOOWR010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38041_length_2683_cov_3.191781"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLB85_05865"
     CDS             <1..437
                     /locus_tag="PLB85_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOU72891.1"
                     /translation="LDEEDSSWLGFEPEASTLVNITEANIIVSGGRGLKDAKNFALLE
                     ELAAALGGAVGASRAAVDAEWIPYSHQVGQTGKTVKPTVYIACGISGAIQHLAGMSSA
                     DYIIAINRDPDAPIFKVADLGIVGDLFEVLPALTKKVKELRG"
     gene            462..1085
                     /locus_tag="PLB85_05870"
     CDS             462..1085
                     /locus_tag="PLB85_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU72892.1"
                     /translation="MSLSVCEIFYSLQGESSFAGRPCVFVRLSGCNLRCSYCDTQYAW
                     GPGKEMEVGEILCEVRKFPTRLVEVTGGEPLWQEDTYALLRDLCKEGYACLLETNGSL
                     WLEDMPDEVIRIIDVKCPGSGCGDSFMKWNLKRLKPRDELKFVLTSPWDYRFALDFIA
                     ANTLQDRTIHFSSVTSVLKPETLARWMLRDGVTAKLQPQLHKILDLK"
     gene            complement(1255..>2683)
                     /locus_tag="PLB85_05875"
     CDS             complement(1255..>2683)
                     /locus_tag="PLB85_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU72893.1"
                     /translation="MLEATELKKIADEIAAAREASREPDFSVCPLARELYKLSQTQTG
                     SISEACRALTDLCLPTRKGAAAEEDNPQAFERDYLNIHAFLNLVRDFELAPAQKDVSI
                     PAFLDYLEDNEGQDSLKQVSVEGGGSLQLLTVHKSKGLQFDRVFLFYNLSSQGGSDHD
                     KLKAYTSFEGADFQDLRDFGLTYHYADVIKASSHKALAKNSEHREMLEEINNLYVAFT
                     RAKTALHLCFAFQGKDGWDKYVEDRKTGKLNLPVLVAAAAEEFFKDETVVPKENGTYV
                     WPATPIQPKKGESREPKAEGVTVEALTRVLPIRETDRFAGLQRNEIDEHKNWKKIWLE
                     DRKNLFGDLAHHYLSFVKQDQPAEHEYAGKQCLARFGSLLTRETIQTRIEILRSNLPT
                     KQVFPSGYDKVFTEFSLWHRGRELRIDRLLLDTANKRALILDYKTGTGREEGQLERYK
                     QALLALPVIRDNGYQVETDFIDLKI"
BASE COUNT          563 a          734 c          754 g          632 t
ORIGIN      
        1 aactggacga ggaagacagc tcctggctgg gttttgagcc ggaagcctcg acgctggtga
       61 acattacgga agccaacatc atcgtctccg gcggacgcgg actcaaagac gccaaaaact
      121 ttgccctgct ggaagaactg gctgccgccc tgggtggagc tgtgggagct tcacgcgccg
      181 cggttgacgc cgagtggatt ccctattccc atcaggtggg gcaaaccgga aagaccgtga
      241 agccaactgt ttacatcgcc tgtggaatat ccggggccat tcagcacctg gccggaatgt
      301 cctccgccga ctacatcatc gccatcaaca gggaccccga cgcccccatt ttcaaggtgg
      361 cggacctggg catcgtgggc gacctctttg aagtgctgcc cgccctcacc aaaaaggtta
      421 aggaactgcg cggatagacc ttagccagag cgctcgaagc cgtgagcctg tccgtctgcg
      481 agatatttta cagcctgcag ggcgaatcca gcttcgctgg ccgtccctgc gtttttgtgc
      541 gcctcagcgg ctgcaatctg cgctgttcat actgcgacac ccaatacgct tggggcccgg
      601 gcaaagagat ggaggttggg gaaatacttt gcgaggtccg aaaattcccc acccggctgg
      661 tggaagttac cggaggcgaa ccgctttggc aggaagatac ttacgccctg ttgcgggacc
      721 tctgcaaaga aggctatgcc tgcctgctgg agaccaacgg ctcactctgg ctggaggaca
      781 tgccggatga ggtgatccgg atcatcgacg tcaaatgccc cggcagcggc tgtggcgatt
      841 ccttcatgaa atggaacctg aagcggctca aaccccgcga tgaactgaag ttcgtgctca
      901 ccagcccctg ggattaccgc ttcgccctgg atttcatagc agctaatacc ctgcaggaca
      961 gaaccatcca cttttcgtcc gtcacctccg ttctgaagcc ggaaaccctg gcccgatgga
     1021 tgctgcggga tggcgtgacg gcaaaactgc aaccgcaact acacaagatt ctggacctga
     1081 aatagcattc tgcaatcaga gagtcgcgct gttttgttac ccactatggg tggaagcagc
     1141 catcatgatt tgctcaattc cttgaaaaat tgtcagatat ggcagggttt gacaatatca
     1201 tagcgtacct ctttcaccgg tatgggatac gctttcttgg cagggtattt gtcctcagat
     1261 tttcaagtcg ataaaatccg tttccacctg atagccgttg tccctgatca ccggaagcgc
     1321 cagcaaggct tgtttgtatc tttccaactg gccttcctcc ctgccagtgc cggtcttgta
     1381 atccaggatc agggcgcgtt tattcgccgt atccagcaag aggcggtcga tccggagttc
     1441 tctgcccctg tgccagagcg agaactcggt gaaaaccttg tcgtagccgg aagggaacac
     1501 ctgcttggtg ggcaggtttg agcgcaggat ctctatccgg gtctggatgg tttctctggt
     1561 gagtagcgaa ccgaagcgcg ccaggcactg tttgccggcg tattcatgct cagcgggctg
     1621 gtcctgcttc acaaagctga gatagtggtg ggcgaggtcg ccgaaaaggt tcttgcggtc
     1681 ttccagccaa atctttttcc agtttttatg ctcgtcgatc tcgttgcgct gaaggccggc
     1741 aaagcggtcg gtttcacgaa ttggcaaaac acgggtcagc gcttccacgg tgactccttc
     1801 ggcctttggt tccctgcttt cccctttttt gggttgaata ggagtggccg gccaaacata
     1861 ggtcccattt tcctttggga ccacggtttc atccttgaag aattcctccg cggcggccgc
     1921 caccagcacg ggaagattca gttttccggt ttttctatcc tccacatact tatcccagcc
     1981 atccttgccc tgaaaggcaa aacagagatg cagtgcggtt ttggcgcggg tgaaagccac
     2041 atagaggttg ttaatttcct ccagcatttc gcgatgttcg ctgttcttag ccagggcttt
     2101 gtggctggaa gccttgatca catcagcata gtggtaggtg agcccaaagt ccctaaggtc
     2161 ctggaaatcc gccccttcga aactggtgta ggccttgagc ttgtcatgat cgcttccgcc
     2221 ttgggaactg agattgtaga aaagaaacac ccggtcgaac tgcaagcctt tggatttgtg
     2281 gacggttagc agttgcaatg aacctccgcc ctccacagag acctgcttga ggctgtcctg
     2341 gccttcattg tcctccagat aatccaggaa cgcggggatg ctcacatctt tctgcgcggg
     2401 ggctaattca aagtcgcgca cgaggttgag gaaggcatga atgttcagat aatcgcgctc
     2461 aaaggcctgg gggttgtctt cctcagccgc ggcgccttta cgggtcggca gacagaggtc
     2521 tgtgagcgcc cggcaggctt cggagatcga tccagtctgc gtttggctaa gtttgtaaag
     2581 ctcgcgcgcg agaggacaga cagaaaagtc aggttctcga gaagcttcac gggcggcggc
     2641 aatttcgtcc gcgattttct tgagctcagt tgcttcgagc ata
//
LOCUS       DAOOWR010000230         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_38696_length_2648_cov_3.191284, whole genome shotgun sequence.
ACCESSION   DAOOWR010000230 DAOOWR010000000
VERSION     DAOOWR010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38696_length_2648_cov_3.191284"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLB85_05880"
     CDS             <1..863
                     /locus_tag="PLB85_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU72894.1"
                     /translation="LKRISYPNAVQDDKNRLLVGAAIGVTGDYLERAKELAKQGVNLL
                     VIDTAHGHHTNIRSAINKVKAACGVEILAGNIATAKAAQFLIDNGADAIKVGIGPGSI
                     CTTRVIAGIGVPQLSAIMDCSEVAAKAGIPLIADGGIKYSGDIVKAIAGGADAVMIGS
                     LLAGTDESPGEYIIYNGRRFKSYRGMGSIGAMRKGSSDRYFQEGTEANKLVAEGIEGM
                     VPYKGPLKDFLFQLTGGLRSGMGYCGAENIAALREKAEFIEISSAGLKESHPHDVNIT
                     KESPNYQARD"
     gene            817..1752
                     /locus_tag="PLB85_05885"
     CDS             817..1752
                     /locus_tag="PLB85_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOU72895.1"
                     /translation="MSTSPRNRPTTRPEIDLGPSLTSLLNAFVYYIRVEKGMAENSVE
                     SYRRDIFDYLGWNGRPIEEHQAEQVSKYLVELMEAGLVNTSIARKRIALNQFFGFLVD
                     SGKDVNVDFDLVPKIKLSKHLPDFLDVETMLRFLDGLPNTTVLQRRNRAMLELLYCTG
                     LRVSELLGLTTHDFNFTENVILVRGKGSKQRFVPFVESVSDIIRDYVDQTRPALLKLA
                     NTDIMFLNNRGGKLSRMGFWKILRQAALEAGLKQEITPHTFRHSFATHLLEGGVNLRI
                     VQVLLGHASLNTTQIYTHVDTRYLVETHRGYHPRA"
     gene            1783..>2648
                     /locus_tag="PLB85_05890"
     CDS             1783..>2648
                     /locus_tag="PLB85_05890"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU72896.1"
                     /translation="MKKFFIAITTVVALLLVFAGCGQKQERADLRKVRVVLDWTPNTN
                     HAGVYVARDLGFYKEQGLDVEIIQPGENTAEKIVALGQAEFGFSYQESVTIARSQGVQ
                     VKSLAAVIQHNSSGFASLKEADVASPKDFSGKRYGSSGWPSELGILKQVMESSKANFD
                     SVEVVHGIADFFTTIGKNADFEWIYYGWDGVQARLKGIDINFIPVRDIDPIFDFYTPV
                     LIASDLMVNSEPELMKKFLAATSRGYELCIKDPARGAEVFSKAVPELDRELVKASLDH
                     LSGEFQADAPRWG"
BASE COUNT          662 a          721 c          731 g          534 t
ORIGIN      
        1 tcctgaagcg gataagctac ccaaacgccg tacaggacga taagaaccgc ctgctggtcg
       61 gagccgcgat cggagtcacc ggcgattatc tggaaagggc aaaggaactt gcgaaacagg
      121 gtgtgaatct gctggtgatc gacaccgccc acggccacca tacaaatatc cgcagcgcga
      181 tcaataaggt taaggccgcc tgtggcgtgg aaattctggc cggcaacata gcgaccgcca
      241 aagccgccca atttctcatc gacaacggcg ccgacgccat caaggtgggc atcgggcccg
      301 gctccatatg caccaccagg gttatcgccg gaataggcgt tcctcagctt tcggccatta
      361 tggactgctc cgaggttgcc gccaaagcgg gaattccgct tattgccgac ggcggtatca
      421 aatactccgg cgacatcgtg aaggcaattg ccggaggagc ggatgctgta atgatcggct
      481 cgttgctggc aggaaccgac gaaagccctg gcgaatatat catttacaac ggccgccgct
      541 tcaaaagcta ccggggcatg ggctccatcg gcgcgatgcg caaaggcagc agcgaccgct
      601 atttccaaga aggtaccgag gcaaacaagc tggtcgccga aggcatcgag ggcatggtgc
      661 cctacaaagg gccactgaaa gactttttgt tccaactcac gggaggcttg cgctccggca
      721 tgggctactg cggcgcggaa aacattgcag ctctgcgcga aaaagccgag ttcatcgaaa
      781 tcagtagcgc cgggctcaaa gaatcgcatc cccacgatgt caacatcacc aaggaatcgc
      841 ccaactacca ggccagagat tgatctcggg cccagcctga ccagcctgct gaacgctttc
      901 gtttactaca tccgggtgga aaaaggcatg gcggaaaaca gcgtagaaag ctatcggaga
      961 gatatctttg actatctggg ctggaacggg cggccgatcg aagagcatca ggcggagcaa
     1021 gtgagcaaat accttgtgga gcttatggaa gcgggtttgg taaatacttc catcgcccgc
     1081 aaaaggatcg ccctgaatca gttttttggg ttcctggtcg atagcggcaa agacgtgaac
     1141 gtggatttcg acctagtgcc caagataaag ctgagcaagc atctgccgga tttcctggat
     1201 gtggagacca tgctgagatt tctggatgga ctgcccaaca caaccgtgct gcagagacgc
     1261 aaccgggcaa tgcttgaact cctttactgc acgggattgc gcgtctcgga actgctgggg
     1321 ctcaccaccc acgacttcaa ttttaccgaa aacgtaatcc tggtgcgcgg caaggggtcc
     1381 aaacagcgct ttgttccttt tgtggaaagc gtttctgata tcatcaggga ctatgttgac
     1441 caaacccgcc ccgccctgct gaaactggcc aacaccgaca tcatgttcct taacaaccgc
     1501 gggggcaagc tttcccgcat gggattctgg aaaatcctgc gccaggctgc tctggaagcc
     1561 ggcctcaaac aggaaataac gcctcacacc ttccgtcatt cctttgccac gcatctgttg
     1621 gaagggggcg tgaatctgcg catcgtgcag gttttgttag gccatgccag cctgaacacc
     1681 acccagattt atactcacgt ggatacgcgc tatttggttg aaacgcatag agggtatcac
     1741 cccagagctt aacaatcaga tcataccaaa gggagcaata aaatgaaaaa attttttata
     1801 gctatcacaa ccgtcgtcgc cttgctgctc gtctttgccg gatgcggtca gaaacaggaa
     1861 agggcggacc tgcgcaaagt tcgcgtggtt ctggattgga ctccgaacac caaccacgcc
     1921 ggtgtttacg tagcgcgcga cctgggattt tacaaagagc agggcctgga cgtggagatc
     1981 atccaaccgg gagagaacac ggcggaaaag atagtggcac tgggacaggc ggagtttggc
     2041 ttcagctatc aggagagcgt caccatcgcc cgctcccagg gtgtacaggt gaaatcactg
     2101 gccgcggtga tccagcacaa cagctccggc ttcgcttcgc tcaaagaagc cgatgtcgct
     2161 tcccccaagg atttcagcgg aaaaaggtat ggcagctccg gctggccttc tgaactggga
     2221 atattgaaac aagtgatgga aagcagcaaa gcgaatttcg acagcgtgga agtggttcac
     2281 ggaattgccg atttcttcac caccatcggc aaaaacgccg atttcgaatg gatctattac
     2341 ggctgggacg gcgttcaagc ccggcttaaa ggcattgata taaacttcat ccccgtccgg
     2401 gatattgacc ccatcttcga tttctacact ccggtgctta tcgccagcga cctgatggtc
     2461 aattctgagc ctgaacttat gaaaaagttc ctggcagcga cctccagggg ctatgaacta
     2521 tgcatcaagg acccggccag aggagccgag gttttttcca aggcggttcc cgaactggac
     2581 cgcgaactgg taaaagccag cctggatcat ctcagcggtg aattccaggc cgacgctccc
     2641 cgctgggg
//
LOCUS       DAOOWR010000231         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39257_length_2617_cov_3.542545, whole genome shotgun sequence.
ACCESSION   DAOOWR010000231 DAOOWR010000000
VERSION     DAOOWR010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39257_length_2617_cov_3.542545"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..449
                     /locus_tag="PLB85_05895"
     CDS             24..449
                     /locus_tag="PLB85_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72897.1"
                     /translation="MKKQVGERFSGIVVSAKSAGLIIRLNEIPISAILRTADLPGGRW
                     QYRDREMRFVSTSSNNYFQLMDKVLVQIMDVSDDIYLELQNVPNAHQHIAMALPKIAT
                     ASSDKSLGQKDRSRQRNAIDYNGGRKKMVKTSKKRKRKR"
     gene            446..1246
                     /gene="murI"
                     /locus_tag="PLB85_05900"
     CDS             446..1246
                     /gene="murI"
                     /locus_tag="PLB85_05900"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU72898.1"
                     /translation="MKKPIGIFDSGVGGLTVYKAIRSAFPKEDLIYFGDTARVPYGPK
                     SHNTIIEYSVQNARFLLQKGIKILIVACNTSSAVALQDLEALTDIPIIGVIGPGAEMA
                     AKLTRSKKIGVIGTEGTVRSEAYSRAISEILPEAEVFSQACPLFVPLVEEGWLEHPTT
                     RQIVHEYLDPYKEVDIDTLVLGCTHYPLLKEMIGEVLGPKVSLVDSADAIANYLRSLL
                     PVDNDGLGGTDKFFVSDNEHKFAQIAGRILGTEPSQLHRVRLFESWFE"
     gene            1269..2102
                     /locus_tag="PLB85_05905"
     CDS             1269..2102
                     /locus_tag="PLB85_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOU72899.1"
                     /translation="MEPLILTAAITGAETTRADQPNLPITPEEQAADALACYEAGARV
                     IHLHVRDDEGNPTQSMERFKAAIEAIKAAAPELVIQISTGGAVGEAFEKRLAPLELKP
                     DMGTLNAGTLNFGDDVFINYPNDIVRLAEAFKVYGVVPEIEVYESGMIDYVAKLVKKG
                     IITHTPLHVQFVLGVPGGMNGTPKNVLYMMHHLKELIPSATWAVAGIGRFHIPASLTA
                     MVNGGHIRVGFEDNIFYHKGVIAESNAQLVARMARIAAEIGRPLATPAQTRSLLGLPE
                     R"
     gene            2102..>2617
                     /locus_tag="PLB85_05910"
     CDS             2102..>2617
                     /locus_tag="PLB85_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOU72900.1"
                     /translation="MVLSDNAKVVLERRYFRKNEAGETIEDWNALISRVAKNIAGEDV
                     EKEKAYKELLDSGYFLPNSPTLMNAGNDLQQLSACFVLPIEDSMESIFETVKNAALIH
                     KSGGGTGFSFSRLREANARVRSTNGVSSGPISFMKVFNAATDAVKQGGTRRGANMAIL
                     NVDHPQILEFIT"
BASE COUNT          694 a          711 c          667 g          545 t
ORIGIN      
        1 aacgcaacta cagcatggcc tacatgaaga aacaagtcgg cgaacgtttc agcggcatag
       61 tggtttccgc caaatccgcc ggccttatca tccggcttaa cgaaatcccc atcagcgcca
      121 tcctgagaac agctgatctt cctggcggca gatggcaata ccgtgacagg gaaatgcgct
      181 ttgtaagcac ttccagtaat aactacttcc agctaatgga taaagtattg gttcagataa
      241 tggatgttag tgacgatata tatttggaat tacagaatgt gccaaacgcc catcagcaca
      301 ttgccatggc attgccaaaa atcgccaccg catcctctga caaaagctta ggacaaaaag
      361 atagatcccg gcaaagaaat gccattgact ataatggcgg gcgcaaaaag atggttaaaa
      421 catcaaaaaa gcgcaagaga aagagatgaa aaaaccgatc gggatattcg attccggagt
      481 gggaggcctc acagtttaca aggcgatccg atccgcgttt ccgaaagaag atctgatcta
      541 ttttggcgac acggcaagag ttccctatgg ccccaaatcc cacaatacca tcattgagta
      601 ttcggttcaa aacgcccgtt tcctgcttca gaaaggcata aagatcctga tcgtagcctg
      661 caacacctct tccgcagtag ccttgcagga ccttgaagcc ttgaccgaca tacccatcat
      721 cggtgtaatc ggccccggcg ccgaaatggc agccaagctc acacgttcaa aaaagattgg
      781 agtgattgga accgaaggca cggtgcgttc tgaagcttac agtcgggcca taagtgagat
      841 cttgccggaa gcggaggttt tttcccaagc ctgtcccctc ttcgttccgc tggtggagga
      901 aggctggctg gaacacccca caacgcgcca gatcgtgcac gaatatcttg atccctacaa
      961 ggaagtggat atcgacaccc tggtgctcgg atgcacccac taccctctgc tgaaggaaat
     1021 gatcggcgaa gtgctcggcc ccaaagtttc cctggtggac agcgccgatg ccatagccaa
     1081 ctatctgagg tcgcttctgc ccgtggataa tgacggcttg ggcggcacag acaagttttt
     1141 cgttagcgac aacgaacata aattcgccca gatagcggga cgcattctgg gcacagagcc
     1201 aagccagctg catcgtgtac gcctgttcga aagctggttt gagtaaaata aaggataaag
     1261 gattttgtat ggaacccctc attttaacag cagcaatcac cggagcggaa acaacccgcg
     1321 ctgaccaacc caacctaccc atcacgcctg aagaacaggc ggcagacgcc ctggcttgtt
     1381 atgaagccgg cgcgagagtg atccacctcc acgtccgtga cgacgaaggc aatcccaccc
     1441 agagcatgga gaggtttaag gcggctatcg aagccattaa ggcagctgcc cccgaactgg
     1501 ttattcagat cagcacagga ggtgctgtgg gcgaagcttt cgaaaagcgt ctggcgcccc
     1561 tggaactgaa accggacatg ggaacgctta acgcaggcac tctgaatttc ggcgatgacg
     1621 tgttcatcaa ctatcccaat gacattgttc gcctggcaga ggctttcaag gtttacggtg
     1681 tggtgccgga gattgaagtt tacgaatccg gcatgatcga ctacgtggcc aaactggtga
     1741 aaaagggcat catcacccac actccgctgc acgttcagtt cgtgctgggt gtacccggcg
     1801 ggatgaacgg aaccccgaag aacgtgctct acatgatgca ccatctaaaa gaactgatcc
     1861 catctgcaac ctgggcggtg gcgggaattg gccgcttcca catccctgcc tcgcttactg
     1921 caatggtcaa tggcggacac atccgcgtcg gttttgaaga caacattttc tatcacaaag
     1981 gcgtgattgc ggaatccaat gcccaattgg tggcacgcat ggcccgcatc gccgcagaga
     2041 tcgggcgtcc ccttgccact ccggcgcaaa cgcgctcctt acttggcttg ccggagaggt
     2101 gatggtccta agcgataatg ccaaagtggt tttggagcgg cgatatttcc gcaagaacga
     2161 agccggcgag accattgaag actggaacgc cctgatcagt cgcgtggcaa agaatatcgc
     2221 cggtgaagac gtggagaaag aaaaagccta taaggaactg cttgattcag gttactttct
     2281 acccaactcc cccactttga tgaacgctgg caatgatctg caacaacttt ccgcctgttt
     2341 tgtgcttccc atcgaagaca gcatggaaag catcttcgaa acggtgaaaa acgccgccct
     2401 tatccacaaa agcggcggtg gaaccggctt ttccttcagc cggctgcgcg aagcaaatgc
     2461 ccgggttcgt tccaccaacg gcgtttccag cggccctatt tctttcatga aagtcttcaa
     2521 cgccgccaca gatgccgtga agcaaggcgg aacccggcgc ggggccaaca tggccatcct
     2581 caatgtcgat catcctcaga tactggaatt catcacc
//
LOCUS       DAOOWR010000232         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39272_length_2616_cov_4.433034, whole genome shotgun sequence.
ACCESSION   DAOOWR010000232 DAOOWR010000000
VERSION     DAOOWR010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39272_length_2616_cov_4.433034"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..365
                     /locus_tag="PLB85_05915"
     CDS             66..365
                     /locus_tag="PLB85_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72901.1"
                     /translation="MNILFRDVQTGIVEARAIMEIADGVFLNEITILNIDGEIVVEFP
                     RKSFLGKNRRTYYMDVITFEDNDKRIVWELEIKSAYREWRKANKKVLVYEDKEQK"
     gene            384..1604
                     /locus_tag="PLB85_05920"
     CDS             384..1604
                     /locus_tag="PLB85_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU72902.1"
                     /translation="MITDLQEILSTNIKGMKRSAIRELLKYLGQPGLISFSGGFPAPA
                     SFPVEELKGIISDVMNNEAAYVLQYGATEGDNLLRELMVERYKKAGFKLGVENIIITT
                     ASQQALDLIAKMFINRGDTVIVGLPSYLGGLSAFNSYGAEMIGIPMDDEGEIPEIMEA
                     KIKEAIARGKKPKFIYVIPDFQNPAGMTMSAKRRREIIDIAHRYDILIVEDSPYRELR
                     YVGEDQPTLFELDGTGHVILLGTFSKIFCPGFRIGWVIGHPDVLDKIVVGKQATDLCT
                     PPFTQRIAARYIEKGYLDPKLDQIRAMYKSKQKVMLDALQEFMPEEFTWIKPEGGLFL
                     MAYGPEGIDTNELLLPCIKEQNVAYVAGTSFFCDGKGHHTMRLNFSYESEENNREGCK
                     RLGTFYKNVLAKKH"
     gene            1651..1950
                     /locus_tag="PLB85_05925"
     CDS             1651..1950
                     /locus_tag="PLB85_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU72903.1"
                     /translation="MPKKVEKDRMGMIIREEDALEKMEVELTEVKAPGDDDSPVLIYY
                     NWCKKCGICVAFCPTGCLGRKPDGSPYVAAPEKCIHCETCDRLCPDFAITGAKDR"
     gene            1961..>2616
                     /locus_tag="PLB85_05930"
     CDS             1961..>2616
                     /locus_tag="PLB85_05930"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU72904.1"
                     /translation="MQKKQITKPAPKAKPTKTASKPAKPETKLGELQSTRERKTVLMQ
                     GNEAVAYGALDAGCNFFAGYPITPSTEIAEILAAELPKRGGVFVQMEDEIASICAITG
                     ASLAGAKALTATSGPGFSLMQEGIGFAKITETPCVVVNVQRVGPSTGMPTSCAQGEIQ
                     QSRWGSHGDSPAIVLYPDSVKECYELTIRAVNLSEKYRTCVILLLDEVLGHMREAVRL
                     "
BASE COUNT          667 a          782 c          672 g          495 t
ORIGIN      
        1 aaaaaaaacc ttgaccaata tagcctgcct tttttaatgc ggtttaatct aagattggag
       61 ttcagatgaa catacttttt cgtgatgtgc aaacgggtat cgtggaagcc cgcgccatca
      121 tggagatcgc cgacggcgtt ttcctcaatg agatcaccat cctcaatatc gatggggaga
      181 tcgtggttga atttccccgc aaaagctttc tgggcaaaaa cagacgcacc tactacatgg
      241 atgtgatcac ttttgaggac aacgacaagc gcatcgtttg ggaactggag ataaagagcg
      301 cttaccgcga atggcgcaag gcgaacaaga aagtactggt ttatgaagat aaagaacaaa
      361 aataaaataa ctggaggatc atcatgatca ccgacctgca agaaattctc tcgaccaaca
      421 tcaagggaat gaagcgctcc gcgatccgcg agctgctgaa gtatttgggc caacccggcc
      481 tgatctcctt ttccggcggt tttcctgccc ccgcctcttt cccggtggaa gaactgaaag
      541 gcatcatctc cgatgtgatg aacaatgaag ccgcctatgt gctgcaatac ggcgccacgg
      601 aaggcgacaa cctgctgcgc gagctgatgg ttgaacgcta caagaaagcc ggatttaaac
      661 taggcgtcga aaacatcatc atcaccaccg cctcccagca ggctctggac ctgatcgcca
      721 agatgttcat caaccgcggc gacaccgtca ttgtgggcct gccctcatat ctgggtggcc
      781 tcagcgcttt caattcctac ggagcggaaa tgatcggcat tccgatggat gacgaaggtg
      841 aaatccccga aatcatggaa gcaaagatca aggaagccat cgcccgtggt aagaagccaa
      901 aattcatcta cgtgatcccg gatttccaaa accccgccgg gatgaccatg agcgccaaac
      961 gccgccgcga aatcatcgac atcgcccacc gctacgacat cctgatcgtg gaagacagcc
     1021 cttatcgcga gctgcgctac gtgggcgaag accagcccac cctctttgaa ctggacggca
     1081 ccggccacgt aattctgctg ggcaccttct ccaagatttt ctgccccggt ttccgcatcg
     1141 gctgggtgat tggccatccg gatgttctgg acaagatcgt ggttggcaag caagccaccg
     1201 acctctgcac cccgcccttc acccagcgca tcgccgcccg ctacatcgaa aagggctatc
     1261 tggatcccaa gctggaccag atccgcgcca tgtataaatc caaacagaag gtgatgctgg
     1321 atgccctgca ggaattcatg cccgaggaat tcacctggat aaagcctgaa ggcggcctct
     1381 tcctgatggc ctatggcccc gagggcatcg acaccaacga gctgctgctt ccctgcatca
     1441 aagagcaaaa cgtggcctat gtggctggaa cctccttctt ctgtgacggc aagggacacc
     1501 acaccatgcg cctcaacttc tcctacgaat ccgaggagaa caaccgcgaa ggctgcaaac
     1561 gcctgggaac cttctacaaa aacgttctgg ccaaaaagca ctgagccgct cccgagactt
     1621 gaaatgaaaa attcacatcc tggaggaaaa gtgcctaaaa aagtcgaaaa agaccgtatg
     1681 ggcatgatca tcagggaaga agacgcgctg gaaaagatgg aggtggaact caccgaagtc
     1741 aaagccccgg gcgacgacga ttcccccgtt ctgatctatt acaactggtg caagaagtgt
     1801 ggcatctgcg tcgccttctg ccccaccggc tgtctgggcc gcaaaccaga cggctcgcct
     1861 tacgtagccg cgccggaaaa atgcatccac tgcgagacct gcgaccgtct ctgccctgat
     1921 ttcgccatca ccggcgccaa agaccgctga ggagagccgc atgcaaaaga aacaaattac
     1981 caaacccgct ccgaaagcca agcccaccaa aaccgcttcc aagccagcga agcctgagac
     2041 caagctcggg gaactgcagt caacgcgcga acgcaaaacc gtgctgatgc agggcaacga
     2101 ggccgtggct tacggtgccc tggatgccgg ctgcaacttc ttcgccggct atcccatcac
     2161 cccttccaca gagatcgccg agatcctggc tgccgaactg cccaagcgcg gcggcgtctt
     2221 tgtgcagatg gaagacgaga tcgccagcat ctgcgccata accggcgctt cccttgccgg
     2281 cgccaaagcc ttaaccgcca cttccggccc cggcttttcg ctgatgcagg aaggcatcgg
     2341 cttcgccaag atcaccgaaa caccctgtgt ggttgttaac gtgcagaggg tggggccctc
     2401 caccggcatg cccacaagct gcgcccaggg cgagatccag caatcccgct ggggcagcca
     2461 cggtgacagc ccggccatcg tgctctatcc tgacagcgtg aaggaatgct acgaactcac
     2521 catccgtgcc gtgaaccttt ctgaaaaata ccgcacctgc gttatcctgc tgctggatga
     2581 ggttctgggc cacatgcgcg aagcagtgag gctgca
//
LOCUS       DAOOWR010000233         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39317_length_2614_cov_3.515045, whole genome shotgun sequence.
ACCESSION   DAOOWR010000233 DAOOWR010000000
VERSION     DAOOWR010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39317_length_2614_cov_3.515045"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="PLB85_05935"
     CDS             complement(<1..1894)
                     /locus_tag="PLB85_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72905.1"
                     /translation="MRRKLWVALLLLFALFAASCNLRENALLPEHLDPLQYVTSNTIK
                     VYKDHLIGSENDKSYLYIPKESIHDYKLWYNDVVRLRRVDSLLERDSLAFAEGVEAIT
                     NCYRISIERNGTEIILDSIPAFATLYTDLKGSKNTSDAWVVRSQYMLYADKFPLETYG
                     KNRVFFEIDGNGDMEVQNPGNAGVFNFPAANTDMEALYYSDTEKLQIWFPQAYLASAG
                     QTRLEILPQLGDADLSAVQAVYPGFALNTKVLDVRTKSAPDASSPPILHWTQPPARKF
                     VQEWVRLQGGRVFGWTQGPETWVVRNNVLISFLKGSGKYFLLSPLGEQEVLELPLDGG
                     FDQLYLQDLWLDLRDTALPNHSLRLNTRPNQASVVSAYFSGAPFTLGSERQAFQLDFS
                     SGNNIVETLPGNNWIEFGFRNQLADTANARLFRVFRSSGSDHIDFKTRGDTYDAHHYS
                     LSSGFVYSGVNSSGTYLLGQVGESSQQQSFPFLKPETSIQTSRGYVSWNDPGTAWGSL
                     LLEYGVAMPSHPWLSGQPYVFSAQNALLRITAYTRGKKRSASLPANTFLVYKHANPLE
                     NLINFSPDADHPRFVWYKAAAAFAHNTFMYHDGALRVSPAWSGYLFSGGSLDPGSASS
                     LRLYPRM"
     gene            complement(1884..>2614)
                     /locus_tag="PLB85_05940"
     CDS             complement(1884..>2614)
                     /locus_tag="PLB85_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72906.1"
                     /translation="APWLGKPRPAVSAGFGYKQNGDTYWLGEASLRLRTYSAALGFEE
                     YRLARQHFRTAYSLDLGKQFTPLDLKLGAKAFSGADAGSYPAWQGSASIAGKAYISSL
                     RLSPLLLFHHTYAPVFSAAQADLGFKISTDRLNLLLMYSGLYRDYLSVGTDQTARVFS
                     GIADLRLYRFLRLSFSASHGDMAWWTNPYGVTLDTMYPNTTNLGLGISIPLGKGFGLS
                     LFSQLGLIDDTRNHLLQGVLSYAP"
BASE COUNT          585 a          643 c          838 g          548 t
ORIGIN      
        1 tcatgcgtgg atagagacgc aggctgctgg cggagccggg atcaagtgaa ccgccactga
       61 agaggtagcc agaccaggcc ggtgaaacgc gcaaagcacc atcatgatac ataaaggtat
      121 tgtgcgcaaa cgcggccgcg gccttgtacc agacgaagcg tggatggtcg gcatccgggc
      181 tgaaattgat taggttttcc agaggattgg catgcttgta aactaggaag gtgttggcgg
      241 gcaggctggc tgagcgtttt ttgccccggg tgtaagctgt gatccgcagc agggcgttct
      301 gcgcggaaaa aacgtagggc tgtccgctca gccagggatg cgaaggcatc gccacaccgt
      361 attctaggag cagcgaaccc caagctgtgc cgggatcgtt ccaggaaaca tagccacggc
      421 tggtttgtat cgaggtttcc ggtttgagga agggaaagct ttgctgctgg ctggattcac
      481 ccacctggcc caacaaatag gtgccggagg aattaacgcc gctgtaaacg aaaccgctgc
      541 tgaggctgta gtggtgagca tcataggtat caccgcgggt tttaaagtcg atgtggtcgc
      601 tgccggaaga gcggaaaacc cggaagagtc tggcgttggc ggtgtctgca agttggttgc
      661 ggaagccaaa ttctatccag ttgttacctg gcagggtttc cacgatgttg tttccgctgc
      721 tgaaatccag ttggaaagcc tggcgttcgc tacccagcgt aaagggcgcg ccgctgaaat
      781 aggcagagac cactgaagcc tgattgggac gggtattcag gcgcagggaa tgatttggca
      841 gggctgtgtc gcgcaggtcc agccacaggt cctgcaggta aagctggtcg aaaccgccgt
      901 ccagagggag ttcaagcacc tcctgttcac ccagcggcga gagcaggaaa tacttgccac
      961 tgccctttaa aaagctgatc agaacgttgt ttctcactac ccaggtttcc ggaccttgag
     1021 tccagccgaa cactcgcccg ccctggagcc gaacccattc ctgcacgaat ttgcgagctg
     1081 gaggctgcgt ccaatgcagg atggggggag aggaggcatc aggcgcagat ttggtcctca
     1141 catccagtac tttggtgttc agcgcgaaac cgggataaac agcctggacg gcgctcaaat
     1201 cagcgtcacc aagctggggc aagatctcca gccgggtctg tccagccgaa gccaggtagg
     1261 cttggggaaa ccagatctgc agcttctctg tatcggaata gtaaagtgct tccatatcgg
     1321 tgttggcggc tggaaagttg aacacgccag cgtttccggg attctgcacc tccatgtcgc
     1381 cgttgccgtc gatttcgaag aaaacgcggt ttttgccata agtctcaagt gggaatttat
     1441 ccgcgtagag catgtactgg ctgcgcacca cccaggcatc cgaagtgttt tttgagcctt
     1501 tgaggtcagt gtagagggta gcgaaggcgg ggatggaatc cagaatgatc tcggtgccgt
     1561 tgcgctcgat gctaatcctg tagcagttgg tgatggcctc cacgccttcc gcgaaagcca
     1621 ggctgtcccg ttccagaagg ctgtccaccc ggcgcaggcg gaccacgtca ttgtaccaga
     1681 gtttataatc gtggatgctt tccttgggga tgtagagata ggacttgtcg ttctcggagc
     1741 cgatcagatg gtccttgtaa accttaatcg tgtttgaggt tacgtactgg agagggtcca
     1801 gatgttctgg caggagggcg ttctcgcgca ggttgcaaga ggcagcgaac agcgcaaaaa
     1861 gcaggagcag ggctacccag agcttacggc gcatagctga ggacgccttg gaggagatgg
     1921 ttgcgggtgt catcgatcag tcctaactgg ctgaaaagtg agaggccaaa ccctttgccc
     1981 aggggaatgc tgatgcccag gccaagattg gtggtattgg gatacatggt gtcgagggtt
     2041 accccgtaag gattggtcca ccaggccatg tccccatggc tggcgctgaa actcaggcgt
     2101 aggaagcggt agaggcgcaa gtccgcgatt ccgctgaaaa cccgggcggt ttggtctgtg
     2161 cccacggaaa gatagtcccg atagagcccc gaatacatga gcagaaggtt gagccggtca
     2221 gtcgagattt tgaagccaag atcggcttgg gcggccgaaa agaccggagc ataggtgtga
     2281 tgaaacaaca ggagcggact gaggcgaagg ctggaaatgt aggctttgcc agcgatggag
     2341 gcgcttccct gccaggccgg gtagctgccg gcatcggctc cgctgaatgc tttggctcca
     2401 agtttgaggt ccaggggggt aaattgtttg cccagatcga gagaataggc ggttcggaag
     2461 tgctgtctgg cgagccggta ttcctcgaaa cccagagccg cggagtaagt tctcaggcgc
     2521 agggaagctt cgcccagcca gtaagtgtca ccattttgtt tgtaaccgaa tccggctgaa
     2581 acagcggggc ggggttttcc cagccagggt gccg
//
LOCUS       DAOOWR010000234         2610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39383_length_2610_cov_3.769863, whole genome shotgun sequence.
ACCESSION   DAOOWR010000234 DAOOWR010000000
VERSION     DAOOWR010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39383_length_2610_cov_3.769863"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1055)
                     /locus_tag="PLB85_05945"
     CDS             complement(99..1055)
                     /locus_tag="PLB85_05945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOU72907.1"
                     /translation="MERITVLKGGDSSERKISLLSGTAVAEGLRVKGYEVTELDPADF
                     STLGQLIERMQELETELVFLGLHGGSGENGQLQAALDLVGLRYTGSGFQACALTMDKY
                     VSKLMAREEGVPVADWVLLRGDQLGDYNDPRDLQGIVDKLGLPLIVKPNDGGSSVGIS
                     KVECVEQLREASQHALKYSSSALLERFIPGRELTATVIDGQALPLVEIKPLSGWYDYD
                     NKYNAGRTEYLAPAPIEESVAQLMQTYAVRLWQVFGLRGYARIDFRYDGEKPYFLEVN
                     TLPGMTTLSLTPMAAKAVGISFVDLVDKIAHLALDQGNTEAT"
     gene            complement(1089..1952)
                     /locus_tag="PLB85_05950"
     CDS             complement(1089..1952)
                     /locus_tag="PLB85_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72908.1"
                     /translation="MPAPPIWIDPRFMLAVQELHQLEALQLLCHKGEQLVAVLPLYEK
                     KSLGLRRLICPMSSYYQGLWFFWEPEREPNRNLLDELKVTSEVAKWLKSRYKRMQFNL
                     SPHNYDVRGFTWQGLKVLPYYTFSYNSAKEMKMLRDEREKLRSARKHDYKLVGEFRPE
                     EFITLIKDLYDRKEKSLGLSFKAFQTWMERLHRDGLLAQFNLMEQDRIVSTNIVLGGI
                     NDQRGYSIMRSTLPSSFKSGASTLHSLLLVEMLKDRFACLDFCGANYPDVARFKAALG
                     LDLEMFFKIVR"
     gene            complement(1984..2346)
                     /locus_tag="PLB85_05955"
     CDS             complement(1984..2346)
                     /locus_tag="PLB85_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU72909.1"
                     /translation="MNTRILLVDDDRLLREVIGDFLRSRGYVVDIAKNRKEAEEHLQK
                     EQYGLALIDHGHRQASGIRLMREIRQAYPNVFCLIMTGYSGVKTVSEAMEEGFTDYIL
                     KPFQLEELINILKKYSYV"
BASE COUNT          595 a          727 c          636 g          652 t
ORIGIN      
        1 gtgttgcctc ccgccaaatg cccgcataaa atgcgggaat catgcgagtg tcatgctaga
       61 tgggaactag caggggttaa tgagggacgg atggtttctt atgtcgcttc tgtgttacct
      121 tgatccaaag ccaggtgggc tatcttgtcc accaggtcca caaagctgat ccccacggcc
      181 ttggctgcca tcggggtcag gctgagcgta gtcatcccgg ggagggtgtt cacttccagg
      241 aaatacggtt tttctccgtc atagcgaaag tctatccgcg cgtagccccg caatccgaaa
      301 acctgccaca gtcgcacagc gtatgtctgc atcaattggg ccactgattc ttcgattggg
      361 gccggggcca gatattcagt gcgtccggcg ttgtatttgt tgtcataatc ataccaacca
      421 ctcaggggct tgatctcaac caaaggcagg gcctggccgt caatcacggt agccgtaagc
      481 tcgcggccag ggatgaagcg ctccaaaagc gcgctggagc tgtatttaag ggcatgctga
      541 gatgcttccc gaagctgctc gacacactcc accttgctga tgccgaccga ggagccgcca
      601 tcgttcggct ttacgatcag gggtagcccc agcttgtcca ctatgccctg aaggtcgcgg
      661 ggatcgttat aatcgcccaa ttggtcaccg cgaagcaaca cccagtccgc aacaggcacc
      721 ccctcctccc tagccatcag ttttgacacg tatttgtcca tggtgagggc gcaagcctgg
      781 aaccccgacc ccgtgtagcg caatccaacg aggtccaggg cggcttgcaa ctgcccgttt
      841 tcaccagaac cgccgtgcaa accaagaaag acgagctcgg tttccaattc ctgcatccgc
      901 tcgatcagtt ggcccaaggt tgaaaaatca gccggatcaa gttccgtgac ctcataaccc
      961 tttactctca gcccctccgc gaccgccgtg ccgcttaaca gcgaaatctt ccgctcggaa
     1021 gagtcgccgc ctttcaaaac tgttattctt tccatgcttc ctctgttttt cagcttatat
     1081 aaaccgcctc agcgcactat cttgaagaac atctccaaat caagacccag agctgctttg
     1141 aaccttgcca catcaggata gttcgcgccg cagaaatcca ggcaggcaaa cctatccttg
     1201 agcatctcca ccaacagcag gctgtgcaag gtgctcgccc cacttttaaa gctactggga
     1261 agggtgctgc gcatgatgga atagccgcgc tggtcgttta tgccgcctaa cactatgttg
     1321 gtgctgacaa tgcggtcctg ctccatcagg ttgaactgag ccaacaggcc gtcccggtgc
     1381 agccgctcca tccaggtctg gaaggctttg aaggacaatc ccaggctctt ttctttgcgg
     1441 tcgtagaggt ccttgatcaa agtgatgaat tcctccggac ggaattcccc caccagcttg
     1501 taatcgtgtt ttctggcgga acgcagtttc tcgcgttcgt ctctcagcat tttcatttct
     1561 ttagcgctgt tgtaggagaa agtgtaatag ggtaagactt tcaatccctg ccaggtgaat
     1621 ccgcggacgt cataattgtg cggcgacagg ttgaattgca tccgtttata gcgggatttc
     1681 agccacttcg ccacttccga ggttaccttg agttcgtcca gcagattgcg gttcggctca
     1741 cgctccggtt cccagaaaaa ccacaaaccc tgatagtaag aactcatggg acagatcagc
     1801 cgcctcaggc ccaggctttt tttttcatac agcggcaaca ccgcgaccag ttgttcgcct
     1861 ttgtggcaaa gcaattgcag cgcttccagc tgatgcagtt cctgaaccgc cagcataaag
     1921 cgcggatcga tccagatggg gggagctggc aaaccctggt cggaaggagc acggaggctt
     1981 atatcataca tatgagtact ttttcaggat gttgatcagt tcctcaagtt ggaagggttt
     2041 gaggatataa tccgtgaaac cttcctccat cgcttcggaa accgttttca cgccggaata
     2101 gcctgtcatg atcagacaaa acacgttcgg ataggcctga cgaatctcac gcatcagtct
     2161 aatcccgctg gcttgcctgt ggccgtggtc gatcagggcc agcccatatt gttctttttg
     2221 caaatgctcc tccgcctcct tgcggttctt ggcgatgtcc acaacatatc ctcttgagcg
     2281 caaaaagtcg cctataactt ctcgcagaag ccggtcgtcg tcaaccagca ggattcttgt
     2341 gttcatttca ccgccttggc ttgggttgat ttttccacac cgcgtcctgt ccttttcaag
     2401 caactgtcgg agtggtgtct ttagtctagc ttgcattttc agaaatcccc acaaaaatgt
     2461 caactaaaat aatccagacc catctgcttt tccccttgct ctgaatttca tttttatatt
     2521 gactgaattc ggactatatt attagtggaa tcacaaatac tgatgaggta ccaatgagag
     2581 gaaaagtcaa gtggtttaac aagaataaag
//
LOCUS       DAOOWR010000235         2609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39402_length_2609_cov_3.850039, whole genome shotgun sequence.
ACCESSION   DAOOWR010000235 DAOOWR010000000
VERSION     DAOOWR010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39402_length_2609_cov_3.850039"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..149
                     /locus_tag="PLB85_05960"
     tRNA            74..149
                     /locus_tag="PLB85_05960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:107..109,aa:Val,seq:tac)
     gene            181..1149
                     /locus_tag="PLB85_05965"
     CDS             181..1149
                     /locus_tag="PLB85_05965"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU72910.1"
                     /translation="MKIAVTGANGFVGSNFANHFHHQGHEVVAIVRPKSPADLLDPAI
                     AIRRTDYQNGLESALEGVEILIHNAGAIRARSFAQMVAANVETTRRVMEAFNRSETAK
                     RLVYISSQAASRPSRANEEVTESEPSAPVDWYGRSKLLAERIIRAECSREWVVVRPVS
                     VYGPGEKDFLQVFKAVKAGISFRIGRREQYLNLIHIDELCEFTELCCSHPGAVGEVFF
                     ASNGKTYTHHEFMAACAKALGRKKTLDIAIPVPLAVLGFHAGELFETLTGKVTLLNKQ
                     KMKEIIGVNWVCSIAKARSILGWDPHPDLEAQLGKTFAWYREHGWL"
     gene            1146..>2609
                     /locus_tag="PLB85_05970"
     CDS             1146..>2609
                     /locus_tag="PLB85_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72911.1"
                     /translation="MKKLVLLAVLILLATALCAQDGPLRLGGKAFVLPEGWPQSYDPP
                     EVLLRVKVNPDSTLGLVAVLDGKTELEPLLSQHLMLYEYISSSDSLAEFDAILEILSP
                     PEIPAQASTREREQLLEEIEETIAQERESLNFRSPLHPPAEQTALNSIGEPYRSGYYF
                     YGLPENTARLSLRGFEQRSSLFSRGFQDLFALGFLNRQESSLEQAYGRIDHPYQVTLS
                     AIEVGIGGYEQLFGRGLLRKNRLFGIDRLQLGFGFLIQDGAWLQCDSGREAVVLDLSL
                     PLGKTTLDLAVTDHRDILSQRYLRYEYWRSQDFSVERRHRTIFAAWRSPWLDLALLHE
                     HDLATAAVFTDTLRDDAFRLRAWKNLRIGALILTPLYERMFVWRDFTLANDDHSDLLG
                     LDIGLETGRIGAEAIVRLKDFQRLQASADLGYSLGQFRFGALGSFRYREPELVLRVPS
                     PYSPGDSLSRVEIRDDAHLGLFLNCLWDQNSLNLSGGR"
BASE COUNT          523 a          810 c          746 g          530 t
ORIGIN      
        1 gcgcactggt tttgggaacc aggagtcgga ggttcaaatc ctctcacccc gaccactttg
       61 gatagattca agtgggcgct tagctcagct ggaagagcgc atgccttaca cgcatgatgt
      121 caggggttca agtcccttag cgcccaccat atctttttat tacagcattt gggaagtact
      181 atgaaaatcg ccgtgactgg ggccaatggc tttgtgggca gcaacttcgc caaccatttc
      241 caccaccagg ggcatgaggt tgtggccatc gtgcgtccga aatcacctgc tgacctgctc
      301 gatcccgcca tcgccatccg ccgcacggat tatcagaacg gtctggaatc tgctttggaa
      361 ggcgtggaaa tccttatcca caacgccgga gccatccgcg cccgcagttt cgcccagatg
      421 gtggcggcga atgtggaaac cacccggcgg gtcatggagg ccttcaaccg ttcagaaacg
      481 gcgaaaaggc tggtctatat tagttcccag gcggcttccc gcccttcccg ggcgaacgag
      541 gaagtgaccg aatccgaacc ctccgcgccg gtggactggt atggccggag caagctgctc
      601 gcggagcgga tcatccgggc tgaatgctcc agggaatggg tcgtggtgcg tcccgtatct
      661 gtttacgggc ctggggaaaa ggatttcctg caggtcttca aagcggtgaa agccgggatc
      721 agcttccgga tcggacgccg ggaacagtat ctgaacctca tccacatcga cgaactctgc
      781 gaattcaccg agctttgctg ctcccatcca ggggctgtgg gcgaagtttt tttcgcctca
      841 aacggcaaaa cctacaccca ccacgaattc atggctgcct gcgccaaagc tttgggcaga
      901 aagaagaccc tggacatcgc catcccggta cccctggccg tgctgggctt ccacgccggt
      961 gaactcttcg aaaccctcac cgggaaagta actttgctga acaaacagaa gatgaaggaa
     1021 attatcggcg tcaactgggt ctgcagcatc gccaaagcca ggtcgatcct gggctgggac
     1081 ccgcatccgg acctcgaagc ccaattgggc aaaacctttg cctggtaccg ggaacacgga
     1141 tggctgtgaa aaagctggtc ctgctggccg tgcttatcct gctggcgaca gccctctgcg
     1201 cccaagacgg gcctctgcgc ctgggcggca aagcttttgt gctgccggaa ggctggcccc
     1261 aaagctacga tccgccggaa gttctgctcc gggtgaaggt gaatcccgac agcaccctcg
     1321 gcctggtggc tgtcttggac ggtaaaacag agctggaacc cctgctcagc cagcatctga
     1381 tgctttacga atacatctcc tccagcgatt ccctggccga attcgacgcc atcctggaga
     1441 tcctctcccc accggaaatc cccgcccagg caagcacgcg ggagcgggaa caactgcttg
     1501 aggaaatcga ggaaacgata gcgcaggaaa gggaaagcct gaatttccgc tctcccctcc
     1561 accctccggc tgaacagacg gccctgaaca gcattggcga gccttaccgc tccggttact
     1621 atttttacgg attgccggaa aacacggcga ggctttccct gagaggcttt gagcagcggt
     1681 cctccctctt ctcaaggggg tttcaggact tgttcgccct gggctttctg aacaggcagg
     1741 aaagctcgct ggaacaggca tacgggcgca tcgaccatcc ctaccaggtg accctcagcg
     1801 ccattgaggt gggcatcggg ggttatgaac agcttttcgg gcgcggactg ctaaggaaaa
     1861 accgcctctt cgggattgac aggctccagc tcggtttcgg cttcctcatc caggacggag
     1921 cctggctgca gtgtgattcc ggccgggaag ccgtggtgct cgacctcagc ctgcctctgg
     1981 gcaaaaccac cctggacctg gcagttaccg atcaccgcga catcctctcc cagcgttacc
     2041 tgcgttacga atactggcgg agtcaggatt tcagtgtgga acgccgccac cgcaccattt
     2101 tcgcggcctg gcgctcgccc tggctggacc tggccctgct ccacgaacac gacctggcta
     2161 cagcggctgt tttcacggac accctgcgcg acgacgcctt ccgcctccgg gcctggaaaa
     2221 acctgcggat cggcgccctg atcctcactc ctctctacga gcggatgttc gtctggcgcg
     2281 atttcaccct ggccaatgat gaccacagcg accttttggg cctggatatc ggccttgaaa
     2341 ccgggcggat cggcgctgag gcgatagtca ggctgaaaga tttccagcgc ctgcaagcct
     2401 ccgccgatct tggctacagc ctgggccagt tccggttcgg ggctctgggt tcctttcgct
     2461 atcgcgagcc cgagctggtg ctgcgggtcc ccagccccta cagccccggt gattctctaa
     2521 gccgtgtgga gatccgggac gacgcccatc tgggcctgtt tctgaactgt ctttgggacc
     2581 aaaactccct gaacctgtcc ggcggccgg
//
LOCUS       DAOOWR010000236         2598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39640_length_2598_cov_3.119151, whole genome shotgun sequence.
ACCESSION   DAOOWR010000236 DAOOWR010000000
VERSION     DAOOWR010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39640_length_2598_cov_3.119151"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..2285
                     /locus_tag="PLB85_05975"
     CDS             201..2285
                     /locus_tag="PLB85_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU72912.1"
                     /translation="MFSGGGARGFAQLGMLKVLEEEGVYPDHVMGSSMGSILGGLYSL
                     GYTAAEVESLMVTLEYSDIIDERQHRPDLYPGDRRWPSYGNLRLALSPKGMPQAPAGL
                     LSGAKIDLGLARLFMPASAHRDFSKLPINFAGLTIDLHTGQLVLHRDGSLGQAVRGAA
                     SIPAIIAPFTYDGRTHIDGGLLQNLPAPQLIDLGADRILALKINTSMRGANPKNFYEV
                     LNHILNIAMHTSIDANVELCDLILEPDLKGVGNLDYPNAHAIAKIGEDYARKNVEVIR
                     AFRDSLLAEGYVFTKPERITELTSVPVREIVTKGNRRVPAARIIALSGLRDDVEYHQD
                     DIVKACVHVWNSCDFHTVYPVLEPLESGYRLVLYVREREPRYLHLNTSYSSDERFSLG
                     AVAEFNDIFMPDSRLLAGFTLGGRNELNLDFVKNFGDFNAPYLRLYPYLRQSTALRYN
                     EAGQRTASLDSLEYGIVPGLGFFADKLFSLEAFGYFSQAKSFAKIPSGAPSSAWQKDA
                     GLGLKIYHESLDNDVFPRSGACFFAKTNIAPWSNLSSKNYARLTADLDVYAPLARKVS
                     LRAGFAYGSHFGGLADNATDLISFGGAETFSGYQKDESPSAEYKYGTLSAVFEPLSNI
                     FLETGAQALNTAGNSDWSLDGSLLFSYYASLGFRSPAGPLGVKFALREDGKANFFFNI
                     GYTTDLFWFSRK"
BASE COUNT          582 a          745 c          666 g          605 t
ORIGIN      
        1 caaaccaacg atcggcaagg taaagattga ggccaccgcg gtgttcggca aggtggagtt
       61 cgtttacaag gaaccaaccc ggccccgtcc tgctaaagca cctggagcgc catctgacag
      121 tctctaaaaa gcttctggcc gcccttccgg ccatcctttt actcaatatc gcctcgctgc
      181 ccgggaaggg gctgggctat gtgttcagcg gcggcggagc gaggggtttt gcgcagttgg
      241 gcatgctcaa agtccttgaa gaggaaggcg tttatccgga ccatgtgatg ggttccagca
      301 tgggctcaat tttgggcggg ctttactctt tgggctacac cgccgctgag gtggagtcgc
      361 tgatggtcac cctcgaatat tctgatatca tcgatgaacg ccagcaccga cccgacctct
      421 atcctggcga caggcgctgg cccagttacg gcaatttgcg cctcgctctc agcccaaaag
      481 gaatgccgca ggctccggca ggccttctca gcggggccaa aatcgatctc ggactggccc
      541 ggctcttcat gcccgcctca gctcaccggg atttttccaa actgcccata aacttcgccg
      601 ggcttaccat cgatctgcac accgggcaat tggtgctcca ccgcgatgga tcattgggac
      661 aggctgttcg cggcgccgcc tccatcccgg ccatcatagc gcctttcact tacgacggac
      721 gcacacacat tgacggcggc ctcctgcaaa accttcccgc gccgcagctt attgatttgg
      781 gagccgacag aatcctggct ctgaaaatca acacctccat gcggggagcc aatcccaaaa
      841 acttttacga ggttttgaac catatcctca acatcgccat gcacaccagc attgatgcca
      901 acgtcgaact ctgcgacctc atcctggagc ctgatctgaa aggcgttggc aacctggatt
      961 atcccaatgc ccatgccatc gccaaaatag gggaggacta tgcccgcaaa aatgtagaag
     1021 tgatcagggc ttttcgtgat tccctgctgg ctgagggcta tgttttcacc aagccggaac
     1081 gaataacaga actcacctca gtgcctgttc gcgaaatcgt caccaaaggc aaccgccggg
     1141 ttccggcggc caggatcatc gccctgtcag gtttgcgcga tgatgttgaa taccatcagg
     1201 acgatatcgt aaaagcctgc gtccatgttt ggaattcctg cgacttccac acagtttacc
     1261 ccgtgctgga acctctggaa agcggttacc gccttgtcct ttatgtaagg gaacgagaac
     1321 cccgctatct gcatctgaac accagctatt cctcggatga aaggttcagc ctgggcgcgg
     1381 tggcggaatt caacgatatt ttcatgcccg attccaggtt gctggccggt ttcaccctcg
     1441 gcggcaggaa cgagctcaac ctggatttcg tgaagaactt cggcgatttc aacgcccctt
     1501 acctgcgctt gtatccttat cttcgccaga gcacggcctt acgttacaat gaagccggac
     1561 agaggaccgc cagccttgat tcccttgaat atgggattgt tcccggtttg ggctttttcg
     1621 ctgacaagct tttcagcttg gaagcctttg gctacttttc ccaggccaaa agcttcgcca
     1681 aaatcccgtc gggcgcacct tccagcgcct ggcaaaaaga tgccggactc ggcttgaaga
     1741 tttaccatga gagcctggac aatgatgtct ttccccgttc cggagcgtgc tttttcgcca
     1801 aaacgaatat cgcgccctgg tccaacctca gttccaaaaa ctatgcccgg cttaccgctg
     1861 acctggatgt gtatgcccct ctggccagga aagtctcact gcgcgcaggt tttgcctacg
     1921 gttcacattt cggcggactg gcggacaatg caacggacct gatcagcttt ggcggggcgg
     1981 aaactttctc cggctatcag aaagacgaat ccccctccgc cgaatacaaa tacggcaccc
     2041 taagcgctgt tttcgagcct ctttccaata tcttcctgga aaccggcgcc caagctctca
     2101 acactgccgg aaactctgat tggagcctcg acggaagcct cctcttcagt tattatgcca
     2161 gtctgggctt tcgcagtccg gcaggacctc tgggagttaa attcgccctg agggaagacg
     2221 gaaaggcaaa cttctttttc aatatcggat acaccacaga cctgttctgg ttctcgcgca
     2281 aataggcttc agacacttag ttccgcccgg gccgcccttt actccagcct attcacctct
     2341 ttttcacaac ccattaagtt cccaatggct cattgggaac ttaatgggtt gtgaattgga
     2401 tgtaagtggg tcagggcaaa tggggtttca cgcagatttc aaaggaagtg gtgctcgctg
     2461 aagcgccgtc aaagaggaag ggaaaaaggt tgatggaagg ctggcgtcga gcgtagcatc
     2521 gacgacagta aagaagtttg gctcctgtgt tatatggaaa ccaaaatgag ttgcatagct
     2581 atttagtcat gaagttgt
//
LOCUS       DAOOWR010000237         2587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_39863_length_2587_cov_3.240916, whole genome shotgun sequence.
ACCESSION   DAOOWR010000237 DAOOWR010000000
VERSION     DAOOWR010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39863_length_2587_cov_3.240916"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLB85_05980"
     CDS             <1..370
                     /locus_tag="PLB85_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU72913.1"
                     /translation="GAAKAIGLVIPELAGKLDGIAIRVPTPDGSLVDLCVNLERETTR
                     EEVNAAVKEAAETYLKGFLQYSEEQLVSIDIVGNPHSSIFDAPSTNVKGKMVKIFSWY
                     DNEWGYSCRVVDLLDYMSKL"
     gene            416..1189
                     /locus_tag="PLB85_05985"
     CDS             416..1189
                     /locus_tag="PLB85_05985"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOU72914.1"
                     /translation="MAKYYYTYEGKTLGPVSPKEVLDLILDDVLSMDSSVMESSSPQW
                     LKIRDIPELMRYLHESEVQITDWAEDKGFASIDKENVPLFFHIPVSRLVWLSLITFGF
                     YEIYWIYRNWRFLRFHRKGRTAAYFGRDLRNPVDLVGVFSQISTDQELGGRYSGRDFT
                     LNGWLWIVALLTQVARSILGFTPFLSVWVDFALTFGLLALSILCLVPVQKHINEGNAK
                     AGKPLSRPTFGHYASIVVGLLVWLLVLLSLIPRLVRLFT"
     gene            complement(1223..2089)
                     /locus_tag="PLB85_05990"
     CDS             complement(1223..2089)
                     /locus_tag="PLB85_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72915.1"
                     /translation="MKRSLSLLVLTALLLLLSGCVEYDEELWLNSNGSGRARMRLVHQ
                     SYYANTQEIMNKLDKPGIHLENITRKQSGPKMIYEVELKFDSIEAFNSVNDQLWSADF
                     WGNITIAKNAKGNIEFKRLISLGSQNAAPEGTGFPGEPGEYNAAGEDSSMFDSDDNLV
                     YDEDYINSLPDDNDILQGIYMQQQTEHPMWTYKLHLPWKIIHAGDNSQSIDHANRVVT
                     WKFDTLNMWNKKELMTVEMKKGLNWMLYALIGLVGTLVLVFVIWMIRINRRSYLQDAI
                     KHQQESEHQQQQ"
     gene            2251..>2587
                     /locus_tag="PLB85_05995"
     CDS             2251..>2587
                     /locus_tag="PLB85_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOU72916.1"
                     /translation="MTELRQIYHCPICGNLVEVLFTGAGALVCCGEPMKLLEGNTVDA
                     ALEKHVPVLTEMGDKVKVVVGSTPHPMEEKHYISMIEVCTKNKVHRHELKPGDAPEAI
                     FRVSADKVVC"
BASE COUNT          537 a          680 c          704 g          666 t
ORIGIN      
        1 cggcgccgcg aaagccatcg gcctggtgat cccggaactg gcgggaaagc ttgacggcat
       61 cgcgatccgc gtgcccactc cggatggttc gctggtggac ctctgcgtga atctggaacg
      121 cgaaaccaca agggaggagg tcaacgccgc cgtgaaagag gccgccgaga cctatctgaa
      181 aggctttctg cagtattccg aggaacagtt ggtctccatc gacatcgtgg gcaatcccca
      241 ttcctccatc ttcgacgccc cttccaccaa tgtgaagggc aagatggtga agatcttcag
      301 ctggtatgac aacgagtggg gctactcctg ccgcgtggtc gatctgctgg actacatgag
      361 caaactatga tttgatccgg gggtggcctt atccgccacc tccgggtttt tatctatggc
      421 gaaatactac tacacttacg agggcaagac tctgggcccg gtaagcccca aagaggttct
      481 ggatctgatc ctggacgatg tgctgagcat ggattcatcc gtgatggaaa gtagttcacc
      541 gcaatggctg aagatacgtg acattccgga actgatgcgt tacctgcatg aatcggaagt
      601 gcagataacg gattgggccg aggacaaagg ttttgccagt atcgacaagg aaaacgtccc
      661 gctgtttttc cacattccgg tgtccaggtt ggtctggctg agccttatca cctttgggtt
      721 ttacgagatt tactggattt acaggaactg gcgttttctg cgctttcacc gtaagggtcg
      781 caccgctgcc tatttcggga gggatttgcg gaatcctgtt gacttggtgg gggtattttc
      841 acaaatttcc acggaccagg agttgggcgg aaggtacagc ggacgggatt tcacactcaa
      901 cggctggctc tggatagtgg cccttctgac tcaggtggct cgctccatcc tggggttcac
      961 gccgttcctg agcgtctggg tggatttcgc gctgaccttc ggcctgctgg ctttatccat
     1021 cctctgcctt gtgccggtgc aaaagcacat caacgagggc aacgccaaag ccggtaaacc
     1081 gctttccagg cctacctttg gacactatgc ctcgatcgtg gtgggattac tggtctggtt
     1141 gctcgtgctg ctttccttga taccccgtct tgtcaggctt tttacctgaa acttcagccc
     1201 tcagcctctt taaaactctt ttctattgtt gttgctggtg ctcgctttcc tgctggtgtt
     1261 tgatggcgtc ctgcagatat gagcggcggt tgatcctgat catccaaatg acgaagacca
     1321 gcaccagggt gcccacgagg ccgatcagcg cataaagcat ccagttcagg ccctttttca
     1381 tctccaccgt catcagctct tttttgttcc acatgttcag cgtgtcgaac ttccaggtta
     1441 ccactctgtt ggcgtggtct atgctctggg agttgtcgcc cgcgtggatg attttccagg
     1501 ggaggtgaag tttgtaggtc cacatcgggt gttcggtttg ctgctgcata tagatgccct
     1561 ggaggatgtc gttgtcgtcc ggcaggctgt tgatgtagtc ttcatcatag acaaggttgt
     1621 cgtctgagtc gaacatggag ctgtcttcac cggcggcatt gtattcccca ggctcgccgg
     1681 gaaatccggt accttccgga gcggcgttct ggctgcccag agaaatcagg cgcttgaatt
     1741 caatgttgcc cttggcgttt ttcgcgatgg tgatgttgcc ccagaaatcc gctgaccaaa
     1801 gctggtcgtt cacgctgttg aaagcctcta tgctgtcgaa ctttagctcc acctcataga
     1861 tcattttcgg cccgctctgc tttctcgtga tgttttccag gtgaatcccc ggtttgtcga
     1921 gcttgttcat gatctcctgg gtgttggcat aataggactg atggaccagg cgcatcctag
     1981 cccggcccga gccgttggag ttcagccaaa gctcttcatc gtattccacg catccggaca
     2041 gaagcaggag cagcgcggtc aataccagta gcgacagcga tcttttcatg gatatcttgt
     2101 tcctcatgca acaaaccttg tgtttccact aatagaataa tcgctttttt ggcaagcaaa
     2161 atatttcccg cgcgaaggct cagatttcta ttgacaaaag atgccgcctt ccggactctt
     2221 gaatttcatc atcaatgtaa ggagttaaac atgacagaac tgcgtcaaat ataccactgc
     2281 ccgatctgcg gcaacctggt ggaagtcctt ttcaccggtg ccggagcgct ggtttgctgc
     2341 ggtgaaccga tgaagctttt ggaaggaaac accgtggatg ccgcgctgga gaaacacgtt
     2401 ccagtgctca ctgaaatggg cgacaaggtc aaggtcgttg tgggttctac accccatccg
     2461 atggaggaaa agcactacat ctccatgatc gaggtctgca ccaagaacaa ggtccaccgc
     2521 cacgaactca agcccggaga cgcccctgag gcaattttcc gcgtgagtgc agacaaagtg
     2581 gtctgcg
//
LOCUS       DAOOWR010000238         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40280_length_2567_cov_3.457404, whole genome shotgun sequence.
ACCESSION   DAOOWR010000238 DAOOWR010000000
VERSION     DAOOWR010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40280_length_2567_cov_3.457404"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2567)
                     /locus_tag="PLB85_06000"
     CDS             complement(<1..>2567)
                     /locus_tag="PLB85_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOU72917.1"
                     /translation="NQLRIACNKAAHEEDYRVSQSTWQSALLAIRDLLLWLAPDQRDE
                     GTDAWLEQHHAEPFRLGDHAPKRSFQCVVRRWELIRKGDRVSGIKIIATDDDGMDCTI
                     FLNDMGGDGRQWSVLNKVLWRFCTLNCLNLSQIHGRPGWYQSNPSTMIVVEPDFLIDA
                     SSVAECFTQKGSRPEFSILNRMVKEAASAKMVLGIMVNSILDDLVFEPNADYQNLFRL
                     AMARQPISLVSLGIDEAQDIYKNIRDNHFPVISKFTGSLQKESVQLEPSYVCPTYGLQ
                     GRLDILHERDGKRHIVELKSGSPPRNNAWQQQLMQVVAYNLIIRDCGGPIPQGYSSIL
                     FSAARENPLRHVGSSVQFEQDLIMCRNRMVGIMHNLALDPNFFFKWLRKNHFTAEPAF
                     LQEKIGNIVKMLNSLQEYEYQWFLEHLRLLMREIWYEKTGGLGPNSIYGYNALWQESR
                     EVKQERYRVLCDLKIEELSVNLLRFKLSGDGAITDFRAGDIIVLYRQDLAVNRQEILR
                     GKIVSIGGDCLEVSIRGGLRQIDARYADHLWSLEHDILESSLYSPLSSIVTFLSAGPE
                     KRSLFLGLSQPRYDDIPELADEPLDQVVQRMFAARDYHIVQGPPGTGKTSGLLTRYIR
                     KIYTETDKTLLILSFTNRAVDEICQNLRKHGIRFIRTGQSEEVEAELLDNLIEGRKFH
                     EIAEVLKSCRVWVATVQSCNAWLSDLLKVVKINELVIDEASQIMENTILGIIARIGKT
                     ILIGDQNQLPPITRQSDEGFSFKHEKLAGLCYDSYGQSLMERLDRVCKEKGWLGSTTM
                     LHQHYRMHDEIADLIQPYYNGRLISALARQKEPLPATGNSLLDSRLVWINFPPSRFA"
BASE COUNT          515 a          687 c          784 g          581 t
ORIGIN      
        1 aggcaaagcg ggaaggagga aagttaatcc agaccagccg ggaatccagc aaagagttgc
       61 ccgtggccgg caaaggctct ttctgcctgg ccagcgcgga aatcaggcgg ccgttataat
      121 agggctggat gaggtccgcg atttcgtcgt gcatgcggta atgctgatgc agcatggtgg
      181 tggacccgag ccagcctttt tccttgcaaa cccgatccag ccgctccatc agcgactggc
      241 cgtaggaatc gtaacatagc ccggccagct tttcgtgctt gaaggaaaag ccttcatcgg
      301 actgccgggt gatgggagga agctggttct ggtcgccgat gaggatggtt ttaccgatcc
      361 tagcgatgat gcccaggatg gtgttttcca taatctgtga ggcttcgtcg atcaccagtt
      421 cgttgatctt caccactttc agcaggtcgc tgagccaggc gttgcagctt tgcacggtgg
      481 ccacccagac gcggcaggat ttcagcactt cggcaatttc atggaatttc ctgccctcga
      541 tgaggttgtc cagcagctcc gcctccacct cttccgactg gccggtgcgg atgaatctga
      601 taccgtgttt gcggagattc tggcaaatct catccaccgc ccggttagtg aagctgagga
      661 taagcagggt tttatccgtt tcggtgtaga ttttgcggat gtagcgggtg agaaggccgg
      721 aggttttgcc ggtgccggga gggccttgca cgatgtggta atcgcgggcg gcgaacattc
      781 tctggaccac ctgatccagg ggttcgtcag cgagctccgg aatgtcgtca taccgcggct
      841 gggagagacc cagaaagagg gaacgctttt ccgggccggc cgaaaggaag gtgacgatcg
      901 agctgagggg gctgtaaagc gaggattcca ggatgtcgtg ttcaagggac cagagatggt
      961 cggcatagcg ggcatcgatc tgcctcaaac cgccccggat ggatacttcc agacagtccc
     1021 cgccgatgct gacgatcttg ccgcggagta tttcctggcg gttcacagcc aaatcctgcc
     1081 tgtaaagcac gatgatgtcg ccggcacgga aatccgttat ggcgccgtcg ccggagagct
     1141 tgaagcgcaa gaggttgacg ctgagttcct cgattttgag gtcgcacagc acccggtaac
     1201 gttcctgctt cacctctcgg ctttcctgcc agagcgcatt gtagccgtag atgctgttgg
     1261 ggccaagccc gccggttttt tcataccaga tttcgcgcat cagcaggcgc aggtgctcca
     1321 gaaaccactg gtattcgtat tcctgcaggg aattgagcat cttcacgatg ttgccgatct
     1381 tctcctgaag gaaggcaggc tccgccgtga aatggttttt gcgcagccac ttgaaaaaga
     1441 aattggggtc aagggcaagg ttgtgcatta tgcccaccat gcggttgcgg cacatgatga
     1501 ggtcctgctc aaactgcaca gagcttccca cgtgacgcag ggggttttcc ctggctgccg
     1561 aaaaaaggat gctgctgtag ccttggggga ttggaccgcc gcaatcgcgg atgatcagat
     1621 tgtaagccac cacctgcatc aactgctgct gccaggcgtt gttgcgcggg ggcgaaccgc
     1681 tcttcaactc cacgatatgg cgtttcccgt cgcgttcgtg caggatgtcc agcctgcctt
     1741 gcagtccgta tgtgggacag acgtagctgg gttccaactg aacggattcc ttttgcagcg
     1801 aaccggtgaa tttggaaatc actggaaagt ggttgtcgcg gatgttctta tagatgtctt
     1861 gcgcttcgtc aatgcccagg gagaccaggc tgataggctg acgggccatg gcaaggcgga
     1921 agaggttctg ataatccgca ttcggttcga agacgaggtc gtccaaaatg ctgttcacca
     1981 ttatccccag caccattttg gccgaggccg cttccttgac catgcggttc aggatgctga
     2041 attccgggcg tgagcctttt tgggtgaagc attccgccac ggaggaggcg tcgatcagga
     2101 aatccggctc caccacgatc atggttgagg gattgctctg ataccagcca ggacggccgt
     2161 ggatttggga aaggttaagg cagttgaggg tgcaaaagcg ccagagcacc ttgttcagca
     2221 cgctccactg ccggccatcg ccacccatgt cattcagaaa gatggtgcag tccatgccgt
     2281 catcgtcggt ggcgattatc ttgatcccgc ttacccggtc ccctttgcgg atcagctccc
     2341 atctccgcac cacgcactgg aagctgcgtt tgggcgcatg gtccccaagg cggaagggtt
     2401 cggcatggtg ctgctccagc caggcgtccg tcccttcatc ccgctggtcc ggggcaagcc
     2461 agagcaagag gtcgcggatg gcgagcaaag cgctttgcca ggtgctctgg ctcacgcggt
     2521 aatcctcttc gtgagctgct ttgttgcagg cgatacgaag ctggttt
//
LOCUS       DAOOWR010000239         2560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40409_length_2560_cov_3.879840, whole genome shotgun sequence.
ACCESSION   DAOOWR010000239 DAOOWR010000000
VERSION     DAOOWR010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40409_length_2560_cov_3.879840"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PLB85_06005"
     CDS             complement(<1..576)
                     /locus_tag="PLB85_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU72918.1"
                     /translation="MERVHNFNAGPAVLPEEVLREAQADLFNYKGMGLSVMEMSHRSK
                     EYQAIIDETNAAVKRIYGLGDDHEVLFLQGGASLQFLMVPMNFAFEGKEANFINTGVW
                     SKKAIAEAKMLGKTVHEAASSADRNFCYIPKTWQLSANPAYLHITTNNTIYGSEWKTD
                     PDVPADVPLIADMSSNFMSKPIDVLKYDLIYA"
     gene            718..1311
                     /locus_tag="PLB85_06010"
     CDS             718..1311
                     /locus_tag="PLB85_06010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOU72919.1"
                     /translation="MIHKLLCDKKIVLASASPRREALLRMLELKPLIIPARVDEPVTG
                     EKPYLQAMRNARSKACAIAEKMDPETLVIGADTVVVLEGAILGKPANSRQAVEYLKTL
                     SGKTHKVYTGLCLCWRKSCVTRYERSLVEFAPLSEAEIQAYVETKEPLDKAGAYGIQG
                     YGSQFIRRVQGCYFNVMGFPIHLFYKMLKELFGDITL"
     gene            1334..>2560
                     /locus_tag="PLB85_06015"
     CDS             1334..>2560
                     /locus_tag="PLB85_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU72920.1"
                     /translation="MKYLYNARFYCEDGFSDRVNALLVDSDKILKLLGPEDPVGSDWE
                     RIDLRGGWVYPGFIDAHTHSFSGGLYENGVDLSNCCSIDEVLSLLFVAARQNPELVIG
                     WRFDEGLIREKRFPTLKELDSVCPGSRLLLRRVDGHSCVLNSKARQAVPGLSSQEEVL
                     IAQDNDLAVNWLQDNCSEETILQAYHSAAQTALRGGFSTIHTMIGDAQQSVQHYSFIQ
                     NRLPEFAINFVLYPQCFNLEAALELGVRRIGGCILADGSIGSRTAALSEAYSAGEGRG
                     NLYQSDQFWREFVMKAHQKGLQVCVHCIGDAAIRQINQAYANLPPDEVRELRHQLIHC
                     EFTPDPLVEEIAASGSVPVMQPAFDMLWGGPDGLYAQRLGNRHQSMNRFRTIAGKGIR
                     VCGSSDWYVTDLNIAMS"
BASE COUNT          563 a          705 c          703 g          589 t
ORIGIN      
        1 ggcgtagatg aggtcatatt tcagcacgtc gatgggtttg ctcatgaagt tggaggacat
       61 gtcggcgatg aggggcacgt cggccggaac gtccggatcg gttttccatt cactgccgta
      121 gatggtgttg ttggtggtga tgtgcagata ggccgggttg gcggaaagct gccaggtttt
      181 tgggatgtag cagaaattgc ggtcagcgct ggaggcggct tcatgcacgg tcttgccaag
      241 catcttggct tcggcgatgg cttttttgga ccacacgcca gtgttgatga agttggcttc
      301 cttaccttca aaggcaaaat tcatcggcac catcaggaac tgaaggctag cccctccctg
      361 aaggaagagc acctcatggt cgtcaccgag gccgtagatg cgcttcacag cggcgttggt
      421 ctcgtcgatg atggcctggt attccttgct gcggtggctc atttccatca cggaaagtcc
      481 catgcctttg tagttgaaca ggtctgcctg ggcttcgcgg agcacttcct ccggaagaac
      541 cgccggacct gcgttgaagt tatgtacgcg ttccattttt acctcctgga acagatattt
      601 ttttactgaa tacaagcttt tcagagggcg tgttttcagg caagcctttt tttcgccttc
      661 aagaaaaatg ttgacgtaat cggctgcccc agcatctgga gattaaggaa caagcacatg
      721 atccacaagc tgttatgcga taagaaaatt gtactggcct ctgccagtcc ccggcgcgaa
      781 gccctgctga gaatgctgga gctaaagccc ctgatcatcc cggccagggt ggatgagcct
      841 gtcaccgggg aaaagccata cctgcaggcc atgcggaatg cccgcagcaa agcctgcgcc
      901 atagccgaaa agatggatcc cgagaccctg gttatcggag ccgataccgt cgtggtgctc
      961 gaaggcgcca ttctgggcaa gcctgccaat agtcgccagg cggttgaata tctcaaaaca
     1021 ctttctggca aaacccacaa ggtttacacc ggcctctgcc tctgctggcg caaatcctgc
     1081 gtcacccgct acgaacgcag cctggtggag ttcgcgcctc tatcagaagc ggaaatccaa
     1141 gcctacgtag aaaccaagga accgcttgac aaagccggcg cttacggcat ccaaggctac
     1201 ggttcccagt tcatccgccg cgtccagggc tgctatttca acgtaatggg attccccatc
     1261 catctcttct acaagatgtt gaaagagctt tttggagaca tcactttata atacatgact
     1321 aaactgattg acaatgaaat acctatataa tgccaggttc tactgcgagg acggtttttc
     1381 cgaccgggtg aacgcccttt tggtcgattc cgacaaaatc ctgaagttgc tcggcccgga
     1441 agaccctgtc gggtcagatt gggagaggat agatctgcgg ggcggctggg tttatcccgg
     1501 ttttatagat gcccacacac acagcttttc cggcggtctt tacgaaaacg gggttgacct
     1561 gtccaactgc tgcagtatcg atgaagtgct ttcactgctt ttcgttgctg cccgtcagaa
     1621 tccggaattg gtgatcggat ggcgcttcga cgaaggcctg atcagggaaa aacgcttccc
     1681 caccctgaag gaactggatt ccgtctgtcc cggttcccgg ctcttgctgc gccgggtgga
     1741 cggtcattcc tgcgtgctca attccaaagc ccgccaagcc gtgccgggcc tttccagcca
     1801 ggaagaagtg ctaattgcgc aggacaacga tctggccgtg aactggctac aggacaactg
     1861 ctccgaggaa accatcctcc aagcctacca cagcgccgcc caaacagcct tacggggagg
     1921 cttttccacc atccatacca tgatagggga cgcgcagcaa agcgttcagc actattcctt
     1981 tattcagaac cggctgccgg aattcgcaat caatttcgtg ctttaccccc aatgcttcaa
     2041 ccttgaggca gctttggaac tgggcgtccg caggattgga ggctgcatcc tggctgacgg
     2101 ttccatcggt tcccgcaccg ccgcgttgag cgaagcttat tccgctggcg aaggccgggg
     2161 aaatctttac cagagcgacc agttctggcg tgaattcgtg atgaaggcgc atcagaaggg
     2221 ccttcaggtc tgcgttcact gcatcggcga cgccgccatc cgccaaatca accaagccta
     2281 cgcgaatctg cctccagatg aggttcggga actgcgccac cagctcatcc actgtgagtt
     2341 tacacctgat cctttggtgg aagaaatcgc cgcttcgggc tcggttccgg tgatgcagcc
     2401 ggcttttgac atgctctggg gcggtccgga tggcctctac gcacagcgtt tggggaacag
     2461 gcaccaaagc atgaaccgct tcagaaccat tgcaggcaaa ggaatccggg tctgcggctc
     2521 cagcgactgg tatgtaacag atctgaacat cgccatgtcg
//
LOCUS       DAOOWR010000240         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40519_length_2556_cov_3.369052, whole genome shotgun sequence.
ACCESSION   DAOOWR010000240 DAOOWR010000000
VERSION     DAOOWR010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40519_length_2556_cov_3.369052"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PLB85_06020"
     CDS             <1..1419
                     /locus_tag="PLB85_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOU72921.1"
                     /translation="FRLALETEDLILAAVDRVRSLPLFYALKNGRLLLSMNAHDLRSR
                     LGDPPLDEQALAEFLLAGYTCLGDTLVSDIKQLQAGQYLIWNKLSQSLRIGEYYVYRQ
                     QFEPLHDSLEALEQMHLRVFERLIQSANGRVLVVPLSGGLDSRLIAVMLKRLGYPKVI
                     CYTFGNSLHDEAVTSRKVAKFLNLPWLIVQQNRHMWYKAYRSEEMRRYFRFGTNLSSL
                     PHVHDWLAIKTLLEREQIPSDAIIVPGHSGGFSQGAGLPLILANREELTRRELLDLIL
                     RDHYDLWPCANDQRHKLFGARVQESLQLPETIQTPIAASFYDEWDWRQRQSRLYVNSL
                     RIYDFFGLDWRMPLWDMDYIGFWTRVPLDLRLGRELMNQYTRKYQSVPLPAYNDYPLG
                     KKLHRRYQRAVSGNLSDQRYGRFLDYRDRNGYLHTKVSTLLAPNLAYPDFVNPDINIL
                     DANINAVQTLTYIRALASGELN"
     gene            complement(1428..2138)
                     /locus_tag="PLB85_06025"
     CDS             complement(1428..2138)
                     /locus_tag="PLB85_06025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU72922.1"
                     /translation="MDYKEHYRQDALVFDYFRRERLTPGEIRRTQYTLSLCEIRPGMR
                     VLDVGSGRGWFSLAAAKLGAEVTALDLSEENLDRIKRENPSIRTVCADACAIPDMGKF
                     DLIVTLEVLEHLIEPELALRSILERLSLDGTLLVTVPYKEVIRYSLCIHCNKKTPMNA
                     HLHSFDRESLSALLVKSGFRTKATKRFYHRALELFKINALCRRFPLGLWKILDRLAGI
                     SGDRYSYLAIKSCPGIQR"
BASE COUNT          542 a          766 c          641 g          607 t
ORIGIN      
        1 tttcgcctgg ccttggaaac tgaggactta atcctcgccg cggtggatag ggtccgctcc
       61 ctgcctctct tttacgccct caagaacggc aggctgctgc tcagcatgaa cgcccacgat
      121 ctccgttccc gcctgggcga tccgcctctg gatgaacagg ccctggcaga gtttttgctg
      181 gccggttaca cctgcctcgg cgacaccttg gtaagcgata tcaaacagtt gcaggccgga
      241 cagtatctaa tctggaacaa gctcagccaa agcctccgga tcggcgaata ttatgtttac
      301 cgtcagcaat ttgagcctct tcatgattcc ctcgaggcgc tggaacagat gcacctgcgg
      361 gtcttcgaac gcctgatcca gtctgcgaat ggccgcgttc tggtggttcc gctcagcgga
      421 gggctggatt cgcgcctgat cgcggtgatg ctcaagcgcc tgggctatcc caaggtgatc
      481 tgctacactt ttggcaattc tctgcatgac gaagctgtaa catcgcgcaa ggtagccaaa
      541 ttcctgaacc tgccctggct gatcgtgcaa caaaaccgcc acatgtggta taaggcctac
      601 cgatcagagg agatgcgccg ttatttccgc ttcggcacca acctcagttc cctgccccac
      661 gtgcatgact ggctggccat aaaaaccttg ctggaacggg aacaaatccc atcagatgcc
      721 attatcgtgc ccggtcattc cggcggattt tcccagggcg ctggcctgcc cttgattctt
      781 gccaaccgtg aggaactcac ccggcgtgaa ctcctggatc taatcctgcg cgaccactat
      841 gacctttggc catgtgccaa cgaccagcga cacaagctct tcggcgcccg ggtgcaagaa
      901 agcctccaac tgccggagac catccaaacc ccgatagcgg cctcattcta cgacgaatgg
      961 gactggcgcc agcgccaaag ccggctctac gttaattctc tacgcatcta cgatttcttc
     1021 ggcctcgact ggcgcatgcc gctttgggac atggattaca tcggtttttg gactcgcgtg
     1081 cccctcgatc ttagactggg ccgtgaactg atgaaccaat acacaagaaa ataccagagc
     1141 gtgcccctgc ccgcctacaa cgactatccg ctgggaaaaa agctgcaccg aagataccag
     1201 cgcgccgtgt ccggaaacct ctccgatcag cgttacggac gctttctgga ttaccgtgac
     1261 cggaacggct acctccacac caaagtgtcc accctgctgg cgccaaacct cgcctacccc
     1321 gattttgtga accccgatat aaacatcctg gacgccaaca tcaacgccgt ccaaaccctc
     1381 acgtacattc gtgccctagc ttccggagaa ttaaactaac ttactgatta tctttgtatt
     1441 ccgggacagg atttgatggc cagatagctg tatctgtcgc ctgagattcc cgccagccgg
     1501 tccaggattt tccacaggcc caggggaaac cggcgacaga gggcattgat tttgaaaagc
     1561 tccaaggccc tgtggtaaaa gcgtttcgtt gccttcgtcc ggaagccgct tttcaccagc
     1621 aaggcactca gggattcccg gtcgaagctg tgcagatgcg cgttcatggg agtttttttg
     1681 ttgcagtgga tgcagagcga atagcggata acttctttgt aaggcactgt caccaaaagc
     1741 gttccgtcca gactgagcct ttccaggatg ctgcgcagag ccaattccgg ctcaatcaga
     1801 tgttccagaa cctccagggt aacgatgagg tcgaattttc ccatgtccgg gatcgcgcag
     1861 gcgtcagcgc aaactgttct gattgatggg ttttccctct ttattctgtc caggttttcc
     1921 tcgctgaggt ccaaagcggt cacctctgcc cccagctttg ccgcggccag actgaaccag
     1981 cccctgccgc tgcccacatc caaaacacgc atccccggtc tgatctcgca tagcgaaagg
     2041 gtgtactgtg tcctgcggat ttcccccggg gtgagtcttt cgcggcgaaa ataatcgaaa
     2101 accagcgcgt cctggcggta atgttccttg tagtccatat tttgttccct ataaaggtgt
     2161 tgcgttcatt gttcagctag cctcaggttg ctggaactac ctcaggctgg ctcgggtact
     2221 ttcccggttg agtcttgctt gattttcaag ctggatgtag cctgccgacg attggtggtg
     2281 cggtcaagga aaatattcaa ccggactcgt attatcctta ttcaacagca cgatagctga
     2341 agaaatgatt tacttttcgg ctcattttca attagagtgg gcaaatccct ccgggctttt
     2401 ttgggtcgag cacagcgctc acttgatttg aaagatagcc ctaattgccc gcccgcagcg
     2461 ctgtcctgcc agccacaccc ttagcgcctt cgtttatccc tctcccacgc ctcccgtatg
     2521 atcctcgcat cgaatgcggg catcatgcgg gaggcg
//
LOCUS       DAOOWR010000241         2555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40533_length_2555_cov_3.326000, whole genome shotgun sequence.
ACCESSION   DAOOWR010000241 DAOOWR010000000
VERSION     DAOOWR010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40533_length_2555_cov_3.326000"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="PLB85_06030"
     CDS             <1..1989
                     /locus_tag="PLB85_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU72923.1"
                     /translation="SALLYASNLGSEELRGRSSSHFTELNLSPMRITASKILPPWTGE
                     FRADVAQLVASVSADSILARIQHLQDFGTRYAYTNTRLPVAQWIQQQFLSYGISDVQL
                     QQFSYNNYQQYNVVATLPGTEYPDQYIIIGGHHDSTSNDKYNFAPGADDNASGAAAVM
                     EIARVMQQNNHQPKTSIRFITFACEELGLWGSYNDSAITYAESLDVRLMINFDMIGNN
                     EHDPSEWMVRQIPYDGSLNFTDYAIGITEQYTSLDSYADMGWYNSSRSDSYPYWQKGY
                     NIVYFFEVDMDSGIYHTIYDVVDSLEIDYCAEVIKAGVACAAIFADLEELSSIPVAIP
                     ATNVSTESFTANWEALPGATGYWLDVYQTGDNGPASDLFFSEYLEGTGSNKALEIYNG
                     TGSAVDLSDYRVEFFPNSYTVPYQSLDLSGELENGEVYVIANPGSFAAIRDRSDLLNS
                     SVTSFDGDDQIALKKLSTGTYVDIFGRIGTDPGSYWGSGTLVTKDRTLVRKSSVTGGV
                     TSNPVSGFPTLVTEWVSYPVNSYHHLGSHSLETVVWVPGYEGLSAGLATSYEVDGLSP
                     GETYCYVVRADLGYGSFGNSNEISVTTGIAAPQPTLTRSANALLLSWPAVNGANAYRV
                     EATDGPGGEYSLVTETTALELEIVSDEPRGFFRVTAIH"
     gene            2173..>2555
                     /locus_tag="PLB85_06035"
     CDS             2173..>2555
                     /locus_tag="PLB85_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU72924.1"
                     /translation="MEQKLIIAIDGPAASGKSTAAQRLALRLGYIYLDTGAMYRACAL
                     QAQISGVSLEDEDAVAAMLKDIDVRIETSGEKNVILLGEKDVSEDIRANAISKLSSDI
                     SALPAVRYRMVELQRQMGARGGVIL"
BASE COUNT          617 a          696 c          654 g          588 t
ORIGIN      
        1 tcagccttac tctacgccag caatctgggc agcgaagaac tgcggggcag atcatccagc
       61 cacttcacgg aattaaacct ctcccccatg cgtatcacag cttcaaaaat cctgccgccc
      121 tggactgggg aatttcgagc cgacgtggct caactggttg ccagcgtcag cgcggattct
      181 attctggccc ggatccagca tctgcaggat ttcggcaccc ggtacgcgta tacgaacacc
      241 cgtctgccgg tggcacaatg gattcagcag cagtttcttt cctatgggat cagcgacgtg
      301 caactacagc agttcagcta caataactac cagcagtaca acgttgtcgc taccctgccc
      361 ggaaccgaat atccggacca gtacataatc atcggcggac atcatgattc caccagcaac
      421 gacaagtata atttcgctcc cggcgctgat gacaacgcca gcggggctgc tgccgtgatg
      481 gagatcgcga gggtgatgca gcaaaacaac catcagccca aaacttccat ccgcttcata
      541 accttcgcct gtgaggaatt gggtttgtgg ggttcttaca acgattctgc aatcacttac
      601 gcggagagtt tggacgtgcg gctgatgatt aatttcgata tgatcggcaa caacgagcat
      661 gacccctctg agtggatggt gcgccaaatc ccttacgacg gttccctgaa tttcacagac
      721 tatgccatcg gcatcaccga gcagtacacc agcctggatt cctatgctga catgggttgg
      781 tacaatagca gccgcagcga ttcctatccc tactggcaaa aaggctacaa catcgtctat
      841 ttctttgagg tggatatgga tagcggaatc tatcatacaa tttacgatgt ggtggacagc
      901 ctggaaatcg attattgcgc ggaagtgatc aaagctgggg tggcctgcgc tgccatcttt
      961 gccgatttgg aggagctttc ctcgatacct gtggcgatcc cggccacaaa tgtcagcacc
     1021 gagagcttta cagccaattg ggaagcgctc cctggcgcaa ccggctattg gctggacgtt
     1081 tatcaaaccg gggataacgg ccccgccagc gatctattct tctcggaata tctggaggga
     1141 acaggctcca acaaagcgct tgaaatatac aacgggacag gatcagctgt ggatctgagc
     1201 gattaccgcg tagagttttt ccccaattca tacaccgtcc cctatcaaag cttggacctt
     1261 tcgggagaac tggaaaacgg tgaagtttac gtgatagcaa accccggttc gtttgcggct
     1321 attcgtgacc gtagcgactt gctaaacagc tcggtcacat cttttgacgg ggacgaccag
     1381 atcgcgctaa agaaactttc caccggcaca tatgttgaca tctttggccg cattggcaca
     1441 gacccgggat cctattgggg atctggaaca ttggtcacca aagaccggac cctcgttcgt
     1501 aaaagcagtg tcaccggcgg cgttacttca aatcctgtca gcggtttccc cacccttgtc
     1561 actgaatggg tcagttatcc cgtcaacagt taccatcact tgggctcgca ctctttggag
     1621 acggttgttt gggtgccggg atatgaagga ctcagtgcag gacttgccac cagttatgaa
     1681 gttgatggtc tctcccccgg tgagacctac tgttacgttg tccgcgccga tctcggttac
     1741 ggcagtttcg gcaattccaa cgaaatcagc gtaaccaccg gcattgccgc tccgcaacct
     1801 accctcacca gatcagcaaa cgctctactg ctcagttggc cggctgtgaa cggcgccaac
     1861 gcttaccggg tcgaagccac ggatggacct ggaggtgaat attcccttgt gacagagacc
     1921 acagcgttgg aactggaaat cgtttcagac gaaccccgag gatttttccg tgtgaccgcc
     1981 atacactgaa acaaagcctc ccagtctgcg ttaactctgt tcgttacaag ttaacagcta
     2041 cccattgttc ctcttggttc cgagcctatt tcttggctat ttcacaaaga atctgcccat
     2101 tggatgaatg ccaaaaaaag gattgacgca atattggggg cgtaaaaata tggtaaaata
     2161 ggataatttg gtatggaaca gaaactcatc atcgctattg acggtcccgc tgcttcggga
     2221 aagagcaccg ccgcacagcg tttggcgctc aggctgggat acatttatct ggatacaggg
     2281 gcaatgtacc gtgcctgcgc tttgcaagcc cagattagcg gtgtgagtct ggaagatgaa
     2341 gatgccgtgg ccgccatgct gaaggacatc gacgtccgca ttgagacctc cggcgagaaa
     2401 aacgttatcc tgctgggaga aaaagacgtt tcggaagaca tccgcgccaa cgccatatcc
     2461 aagctttcct ctgatatttc agccctcccc gccgtgcgtt accgcatggt ggaactccag
     2521 cgccagatgg gagccagggg cggcgtgatc ctgga
//
LOCUS       DAOOWR010000242         2552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40592_length_2552_cov_3.122547, whole genome shotgun sequence.
ACCESSION   DAOOWR010000242 DAOOWR010000000
VERSION     DAOOWR010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40592_length_2552_cov_3.122547"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1286
                     /gene="buk"
                     /locus_tag="PLB85_06040"
     CDS             207..1286
                     /gene="buk"
                     /locus_tag="PLB85_06040"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOU72925.1"
                     /translation="MSQLILAINPGSTSTKIAVYDGDRPVFEKTLRHDPAELEPYPNI
                     IDQYEFRKKLVLEAMQENQIDPHDLAAVVGRGGLVKPVPGGTFKINDAMKRDLRDPSL
                     WGRTHASALGAFIADAISQELDIPGFIVDPVVVDEFDDLARVSGIPEIERKSLLHALN
                     IRYIARLMAAELGKKLDEVNLIGVHMGGGISIAAIKNGRVADVNNALLGMGPFSPQRA
                     GALPIGDLLELAYSGKYTHSQLIKYLTKTAGWMAYLGTDSGIEVNERIQAGDAKAKLI
                     TDAMCYQVAKEVGACATVLKGKVDAIFFSGGLVYNEYIVQQIRSRVEFIAPIKLYPGE
                     REMEALCQGGLRVLNGLEEAMDYNY"
     gene            1310..>2552
                     /locus_tag="PLB85_06045"
     CDS             1310..>2552
                     /locus_tag="PLB85_06045"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOU72926.1"
                     /translation="MKRLLLLALWSVLLFPLSAADWTVLVYMAADNNLWQNAVADVNS
                     MESVSLPANLNLIVQTDMPADSGYPGGQRRKIRPDNSPSITSPLLESLGTIDSGDPQT
                     LASFANWGFQKYPSQRRMLVIWGHGDNWFKADEGKWICPDEGAQSLISVSDGELKEAL
                     SGLPRLDILLFDACSMQSLEVLAEVGQAADIVIASEELVPAAGFPYQTIVPLFADGGV
                     EEIAGQIVEEYLESYLPGGIQNPYGFTNPITCSAVRTSSLGVFFSGFRDFFLSKSHYW
                     PTSMLPIRAKCWEMGTGYNDIDVGELLFRMDEAWDDLLEPGLAPLKDKWKACVVASGS
                     LNILHDVGSAAIWFPRTQQYYDGLWRRYAKLEFARYRWFQILHRVFGPHGKPPSPELV
                     SQGMLLSNLRLELKQPDYPDLL"
BASE COUNT          572 a          688 c          753 g          539 t
ORIGIN      
        1 ggaacttaag gggaggttta cgggatgtta gtgggaaggg gcaaacggcc tgtcgggttt
       61 ctacagcaat acatattatg aggctctctt tcgaacctat ggggtaacac tgaggttttt
      121 agccaggagg gcaaaggaaa agattgacaa aaggaggctg atgccagagc ttgcaagaaa
      181 acaaaaaaca gaggacagga ggataaatgt cccagctaat ccttgccatc aatcccggct
      241 ccacatccac gaaaatcgct gtttatgacg gcgacaggcc ggtctttgag aaaacgctgc
      301 gccacgatcc ggctgagctt gagccctatc ccaacataat cgaccagtat gagtttcgca
      361 agaaactggt gctggaggcg atgcaggaaa accagattga tccgcatgac ctggcggccg
      421 tggtaggccg tggcggactg gtgaaacccg ttccgggcgg aactttcaag atcaacgacg
      481 cgatgaaaag ggacctgcgc gacccttcac tctggggccg cacccacgct tcggcgctgg
      541 gcgctttcat tgccgacgcc atctcccagg aattggacat acccggtttc atcgtggacc
      601 cggttgtggt ggacgaattt gacgatctgg cgcgcgtttc cggcattccg gaaatcgagc
      661 gcaaatccct gctgcacgcc ctcaacattc gctacatcgc gcgtttgatg gcggcggaac
      721 tgggcaaaaa actggatgag gtgaacctta tcggcgtgca catgggcggc gggatttcca
      781 tcgcggcgat caaaaacggc cgtgttgctg acgtgaacaa cgccctgctt ggtatggggc
      841 ctttttctcc tcagcgggct ggcgccctgc ccatcggcga tttgctggaa ctggcttaca
      901 gcggcaaata cacgcattcc caactgatca aataccttac caagaccgcg ggctggatgg
      961 cctatctggg cacggacagc ggcatcgagg tgaacgaacg catccaggcc ggtgacgcca
     1021 aagcgaaact gatcacggac gccatgtgct accaggtggc caaggaagtg ggggcctgcg
     1081 cgaccgtatt gaaaggcaag gtggacgcca ttttctttag cggcggactg gtctataacg
     1141 aatacatagt gcagcagata cgctcccggg ttgaattcat cgcgccgatc aagctctatc
     1201 ccggcgaacg ggagatggaa gccctctgcc agggcggttt gagggttctg aacggcctgg
     1261 aagaggcaat ggattacaac tattgagtga aaggacgcgg ggtgaaactg tgaaacgctt
     1321 gctgctgctg gcactgtggt ccgttttgct gtttcccctc agcgccgcgg actggaccgt
     1381 gctggtctat atggctgcgg acaacaacct ctggcaaaac gcagtggcag atgtcaattc
     1441 gatggaatca gtgtccctgc cggcgaacct caatctgatc gttcagacag acatgcccgc
     1501 ggattcaggc tatcctggtg gccagcgcag aaagatccgt ccggacaatt cgccgagcat
     1561 cacctctccg ctgctggaaa gcctcggcac catcgacagc ggcgaccctc aaaccctggc
     1621 cagctttgcc aactggggtt ttcagaagta tccctcccag cgcaggatgc tggtgatttg
     1681 ggggcacggg gacaactggt tcaaagccga cgagggaaaa tggatttgcc ctgatgaggg
     1741 tgcccaatcg cttatcagcg tgtctgatgg ggaactgaaa gaagccctga gcggccttcc
     1801 gcgtctggat atcctactct tcgacgcctg cagcatgcag agtttggagg tattggcgga
     1861 ggtggggcaa gcggcagaca ttgtgatcgc ttccgaagaa ctggtgccgg cggctggatt
     1921 tccctatcag actattgttc ctctgttcgc ggacggaggg gtggaagaaa tcgccggcca
     1981 gatagtcgaa gaatatctgg aatcctacct cccgggcgga atccaaaacc cgtatggctt
     2041 caccaatccc atcacctgtt cggcggtcag gacctcctct cttggcgtct ttttcagcgg
     2101 cttccgggat ttcttcctga gcaaatccca ttactggccg acatccatgc tgccaatccg
     2161 ggccaaatgc tgggaaatgg gaaccggcta caacgacatc gatgtgggcg aactactttt
     2221 caggatggac gaggcctggg acgatttatt ggagccaggt ttggccccgc tgaaggataa
     2281 atggaaagcc tgtgtggtgg cttccggcag cctcaacatc cttcacgatg taggttctgc
     2341 cgcgatctgg tttccgcgca cccagcagta ttacgacggt ttgtggcgcc gctatgccaa
     2401 gctggaattc gcccggtacc gctggttcca aatcttgcac cgggtttttg ggccacacgg
     2461 aaaaccgccc tctccggagc tggtgagcca gggcatgctg ctatccaacc tgcggctgga
     2521 gctgaaacag ccggactacc ctgatttgct tt
//
LOCUS       DAOOWR010000243         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_40826_length_2541_cov_3.005632, whole genome shotgun sequence.
ACCESSION   DAOOWR010000243 DAOOWR010000000
VERSION     DAOOWR010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40826_length_2541_cov_3.005632"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLB85_06050"
     CDS             <1..506
                     /locus_tag="PLB85_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU72927.1"
                     /translation="DKEGLKAADAPFKIMTYTEAVDYLRSQGSEIEHGSDLGAADEVL
                     LTAGSPVPVFIEKWPRQIKAFYMKRDPHNPDLVLGSDLIAPEGFGEIIGGSQREDDHN
                     LLLWRMQAENMPIEDYQWFLDLRKYGSVPHSGFGVGLERVVAWMSGVHHIRETIPFPR
                     MIYRIYP"
     gene            668..1870
                     /locus_tag="PLB85_06055"
     CDS             668..1870
                     /locus_tag="PLB85_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOU72928.1"
                     /translation="MNYTNTFSKVTKAMKSSLIRELVASTKNIPNLISFAGGFPSPKT
                     FPYAQLSEIFSDVVQNEGRDVLQYGASEGDTLLKQQLMKWEGYDISPDEMIITVGATN
                     ALYYYGKTLVDPGDVVLCEAPTFLGSLVAFDALGADCQTVPFDNDGIIMEQMDAKVRA
                     LRQQGKKIKFLYIIPDFHNPGGISYSIERRRELIRFCLTNEIPIVEDNPYSRLRYSGE
                     PLPTFYRLAHEEFSGTSIVTEIVSFSKILGPGMRIAFAKGDKDLITKMVSWQQKVNIT
                     PDCVAQRVVARFLEQGLMDPHIESIRAFYAPYLAKMLSDLEAFMPPSVSWTKPKGGIF
                     LWMTLPEQVNADELFVKAAQNKVSFIPGSKFYPEGQERFNTLRLNYSFATPEQIETGI
                     RRLAELLA"
     gene            1894..>2541
                     /locus_tag="PLB85_06060"
     CDS             1894..>2541
                     /locus_tag="PLB85_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU72929.1"
                     /translation="MDKFPATRRMFITLLALLALLPASLVAEMLTHRVVSGDTLYNIS
                     KRYGVSIEDIKALNNLRDNTISLNQVLKIKEVDRYAAIVAQPEPQSGFAAGAKLEADA
                     NIPMFTASIDAEGNWLNIRRLPANALHAEDAAGNRYYAGAFSQKTEFGQITLQARTAS
                     SGLTDTWLACQDSAGKWLWAKNLAFVKDEGEPQLALAVGSSGSVCVAGAFSGKTEL"
BASE COUNT          599 a          773 c          660 g          509 t
ORIGIN      
        1 gggacaagga agggctcaag gccgcggacg ccccctttaa aataatgacc tacaccgagg
       61 ccgtcgatta tctgcgctcc caaggcagcg agatagaaca cggcagcgat ttgggtgccg
      121 cggacgaagt tttgctaacc gccggttcgc ctgtgcctgt cttcatagag aaatggccca
      181 ggcaaatcaa agctttttac atgaaacgcg atccccataa ccccgatctg gtgctgggca
      241 gcgacctcat cgcccccgaa ggtttcggag aaatcatcgg cggctcgcag cgcgaggacg
      301 accacaacct cctgctctgg aggatgcagg ccgaaaacat gcccattgaa gactatcagt
      361 ggtttctgga cctgcgtaaa tacggcagcg tgccccacag cggttttgga gtgggtttag
      421 aacgcgtggt cgcctggatg agcggagtcc accacatccg cgagaccata cctttcccca
      481 ggatgatata caggatatat ccctgagcta taacgttttg gagtggccgg ctccgccgct
      541 ggccaaagcc cgtggttttt ttgctgaagc aaaactcctg cgtggctccc atcacgaaag
      601 ctggcgcgac ggatattctc cagcctgatg aatgatatta aataaacaga taaatggagt
      661 aattaggatg aactacacca ataccttctc caaggtgaca aaagcgatga aatcctcgct
      721 catccgtgaa ctagtggcct ccaccaagaa cattcccaac ctgatctctt tcgccggagg
      781 ctttccctcc cccaaaacct ttccctacgc ccaactgtcg gaaatattca gcgatgtggt
      841 ccagaatgaa ggccgcgacg tgctgcaata cggcgccagc gaaggcgaca ccctgctcaa
      901 gcagcagctt atgaaatggg agggctatga catcagcccg gacgagatga tcatcaccgt
      961 gggcgccacc aacgcccttt attattacgg caaaaccctg gtcgatcccg gcgatgtggt
     1021 cctctgcgaa gcccccacct ttttgggctc gctggtggcc tttgacgccc tgggagcaga
     1081 ttgccagacc gtcccctttg acaatgacgg catcatcatg gaacagatgg acgcaaaggt
     1141 ccgcgccctt cgccagcaag gcaaaaaaat caagttcctc tacatcattc cggatttcca
     1201 caatcccggc ggcatcagct acagcatcga gcggcggcgg gaactcatcc gtttctgcct
     1261 gaccaacgaa atccccatcg ttgaagacaa cccctattcc cgcctcagat acagcggcga
     1321 acccctgccc accttttacc gcctggccca tgaggaattt agcggaactt ctatcgttac
     1381 ggaaattgtg tccttctcga agattctcgg cccaggaatg cgcatcgcct ttgccaaagg
     1441 agacaaagac ctgatcacga aaatggtatc ctggcagcaa aaggtgaaca tcaccccgga
     1501 ttgcgttgcc cagagggttg tcgcccgctt tttggagcag ggcctgatgg acccccacat
     1561 cgaaagcatc cgcgctttct atgcgcccta tctggcaaaa atgctttccg acctcgaagc
     1621 cttcatgcct cccagcgtga gctggaccaa gcccaagggc ggcattttcc tctggatgac
     1681 cctccccgaa caagtcaacg ccgatgagct gttcgtcaag gccgcccaga acaaggtttc
     1741 cttcatcccc ggctccaagt tctatcccga ggggcaggaa cgcttcaaca ccctgcggct
     1801 caattacagc ttcgccactc cggagcagat agaaacaggc atccggcgcc tggctgaact
     1861 gctggcttaa tatatctagg tttgtccatg agaatggata agttcccggc cacgcggcgg
     1921 atgttcatca ccctactggc gctgcttgcc ttgctgcctg cgtcattggt tgcggagatg
     1981 ctcacccacc gcgtggtttc cggcgacacc ctttacaaca ttagcaagcg ctacggcgtt
     2041 agcatagagg acatcaaggc cctgaacaat ctgcgcgaca acaccatcag cctcaaccag
     2101 gtgctcaaga tcaaggaagt ggaccgctac gcggcgatag tggcccagcc ggaaccccaa
     2161 agcggatttg ccgccggggc aaagcttgaa gccgacgcga acattcccat gttcaccgcc
     2221 agcatagatg ccgagggcaa ctggctgaac atccgccggc taccggccaa cgccctgcac
     2281 gctgaagacg cggccggaaa ccgttactac gccggggctt tctcccaaaa gacggaattc
     2341 gggcaaatca cactgcaggc acgaaccgcc tccagcggcc ttacggatac ctggctggcc
     2401 tgtcaggaca gcgccggaaa atggctctgg gccaagaacc tggcctttgt gaaggatgaa
     2461 ggcgaaccgc agcttgcttt ggctgtgggg tcttcaggct ctgtttgtgt agcgggtgcc
     2521 ttcagcggca aaaccgagct c
//
LOCUS       DAOOWR010000244         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41071_length_2530_cov_3.216162, whole genome shotgun sequence.
ACCESSION   DAOOWR010000244 DAOOWR010000000
VERSION     DAOOWR010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41071_length_2530_cov_3.216162"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLB85_06065"
     CDS             complement(<1..859)
                     /locus_tag="PLB85_06065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU72930.1"
                     /translation="MKRIAMLGPAPPFRGGISQFALMLAREFQDQGHEVRMFTFVRQY
                     PKLLFPGGEQTTSFAEMPELPVERLFTPYLPQTWPRAVKRIRDYKPDLLLTSYFLPWF
                     APSHAWICKRLPDTRRICLAHNIDFHEKWPGADILSRRLFQHNDRIVLLSQACLEDLK
                     RKMPKDISRKGLLGFHPIYHCYGTGRSESGAEKLEEPTALFFGLIKAYKGLDILLAAL
                     KQARRQVPDLKLIVAGEVYGKKEKYEHLIRELGLSEAVDARFHYITDTEVAEVFRRAR
                     VCVLPYKSAT"
     gene            complement(860..1852)
                     /locus_tag="PLB85_06070"
     CDS             complement(860..1852)
                     /locus_tag="PLB85_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOU72931.1"
                     /translation="MQSETPKKNILIILTGGTISMSSKGSLGVVPSSELADLLREFPQ
                     LDSVANVDVMEYLNLPSPYITPQMMLELAKLIDLKIIDYDGVVITHGTDTLEETSFLC
                     DLVLTTRKPVVFTAAMRSGSDIGLDGPRNIIGAVRVASHHDSADKGVLVVMNDEIHTA
                     RDVVKSDTGKVDAFRSVGYGPLGSVDPDTIVYHRDSLFREYVWTDKLDTAVDLIKAVA
                     GMDGKHIHSSIEGGAKAIVIEAFGRGNLPQSLIPDIQAALDKNILVVISSRTYTGRVL
                     SEYGYEGGGKHLADLGCILAGDLKGVKVRLKLMTLFGKYNDPDVVKRFFMQSRN"
     gene            complement(1852..>2530)
                     /gene="queC"
                     /locus_tag="PLB85_06075"
     CDS             complement(1852..>2530)
                     /gene="queC"
                     /locus_tag="PLB85_06075"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOU72932.1"
                     /translation="KKRMKRAIVLVSGGMDSLVSTAAATRDCEELNFLHVSYGQRTQE
                     RELKSFEAISAHYGPRRSEVLNWDWLSRIGGSALTDPNKEIPNQDPGKELPSTYVPFR
                     NANLLCAAVSWAEAIHADAIYIGAVEEDSSGYPDCREVFFKAFEEVIASGSRNAWPIR
                     IVTPVLHLSKAEIVKLGMELKAPFELSWSCYADNTAACGVCESCRLRLKAFAEAGYKD
                     PITYRGQ"
BASE COUNT          506 a          742 c          674 g          608 t
ORIGIN      
        1 gggtggcgct tttgtagggc agaacgcaaa cccgcgcccg ccggaaaact tctgctacct
       61 cagtgtctgt gatatagtgg aaacgggcgt ccacagcctc gctcaaaccc agttcgcgga
      121 tcaggtgttc gtatttttcc ttcttgccat agacctcgcc ggcaactatc agtttcaggt
      181 ccggaacttg cctgcgtgcc tgtttgagtg ccgcaagcag tatgtccaaa cctttgtagg
      241 ctttgatcag tccaaaaaac agggcggtcg gttcttccag cttttcagcg ccggattcac
      301 tcctgcccgt tccgtagcag tggtagatgg ggtgaaatcc cagcagtcct tttcgggaga
      361 tatctttggg catttttctt ttcaggtctt caaggcaggc ttgggaaagc agcacgatcc
      421 tatcgttgtg ttggaacaac ctgcgggaca ggatgtcggc cccgggccat ttctcgtgga
      481 aatcgatgtt gtgggccagg cagattctgc gcgtgtccgg caaacgctta cagatccagg
      541 catgggaggg cgcgaaccaa ggcaggaaat acgaagtgag caaaagatcg ggcttataat
      601 cccggattct cttcaccgcc ctgggccagg tttggggcag gtagggcgtg aatagccttt
      661 ccaccgggag ttccggcatt tcggcaaaac tcgtggtctg ttctccgcct gggaacagca
      721 gtttgggata ctgcctcaca aaagtgaaca tccgcacttc gtgcccttgg tcctgaaact
      781 cccgcgccag cattagcgcg aactgcgaaa tcccgccccg gaaaggcggc gccggcccca
      841 gcatcgcgat tctcttcatc taattcctgc tttgcatgaa aaagcgtttc accacgtccg
      901 gatcgttgta tttgccaaag agcgtcatta gtttcagcct tactttcacg cctttgaggt
      961 cgcccgcgag gatgcagccg aggtcggcaa gatgcttgcc cccgccttca tatccatatt
     1021 ccgaaagcac tctgccagta taggtccgcg aactgatcac caccaggatg tttttatcca
     1081 gggcagcctg aatgtccgga atcaggctct ggggcaggtt tccgcgccca aaagcctcga
     1141 tcacgatggc cttggcgccc ccctctattg agctgtggat gtgctttccg tccatgcccg
     1201 ccaccgcttt gatcaggtcc accgcggtgt ccagcttgtc cgtccacaca tattcccgga
     1261 acagcgaatc gcggtggtaa acgatggtgt caggatccac gcttcccagt ggaccgtaac
     1321 ccacgctgcg aaatgcgtcc accttcccgg tgtcggattt cacaacgtcc cgggcggtgt
     1381 ggatctcgtc attcatcacc accaacaccc ctttgtccgc agaatcgtga tgtgaagcca
     1441 ctctcacggc cccgatgatg ttgcgcgggc catccaggcc gatgtcactg ccgctgcgca
     1501 tggcggctgt gaagaccacc ggtttgcggg tggtgagcac caaatcgcac aaaaaggaag
     1561 tttcttccag cgtgtcagtg ccgtgtgtga tcaccacgcc atcataatct atgattttca
     1621 agtctataag tttggccagt tccagcatca tctgcggcgt tatgtaaggg ctcggcaggt
     1681 tcagatattc catcacgtca acgtttgcca cgctgtccag ctggggaaac tcacgcagca
     1741 ggtcggccag ttcagagctg ggaaccactc ccagcgaacc tttggaggac atcgaaatgg
     1801 tgcctccggt gagaattatg aggatgtttt ttttgggtgt ttctgattgc attattgtcc
     1861 ccgataagtg atggggtctt tgtatcccgc ttcggcaaag gctttcaggc gcagtctgca
     1921 actctcgcaa accccacaag ccgctgtgtt gtccgcgtaa cagctccagc tcagttcaaa
     1981 cggcgctttc agctccatgc ccagcttcac gatctccgct ttgctgaggt gcagcaccgg
     2041 agtgacgatt cggatcggcc aggcattcct gctgccgctg gcgatcactt cctcaaaagc
     2101 cttgaaaaac acttcccggc aatccggata gccgctgctg tcttcttcca ctgccccgat
     2161 gtagatggca tctgcgtgga tcgcttcagc ccaggatacc gccgcgcaga gcagattggc
     2221 gttgcggaag ggaacatagg tggaggggag ttccttgcct ggatcctgat ttggtatctc
     2281 tttgttcgga tcggtcaaag cagagccgcc gatgcgggac agccagtccc agttcagcac
     2341 ttcgctgcgg cggggaccgt agtgagcgct gatcgcttca aagctcttca attcacgctc
     2401 ctgtgtccgc tgaccgtaac tcacgtgcag gaaattcagc tcttcacagt cccgcgtggc
     2461 cgccgccgtg ctaaccaggc tgtccatccc tccgctcacc aaaactatcg ctctcttcat
     2521 ccttttcttc
//
LOCUS       DAOOWR010000245         2513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41423_length_2513_cov_3.958096, whole genome shotgun sequence.
ACCESSION   DAOOWR010000245 DAOOWR010000000
VERSION     DAOOWR010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41423_length_2513_cov_3.958096"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1005)
                     /locus_tag="PLB85_06080"
     CDS             complement(91..1005)
                     /locus_tag="PLB85_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72933.1"
                     /translation="MKKCLALLIALWMMLTTAAGLSQKLVEADDLSVGDRFVLEIRHD
                     TDLKSVAVPDTLTRFHVLGWQLQKVPRGNDWLKVTIVPLYPGSHSFPALTVRTAEAAS
                     ELLFTDRFRLNIIPVRAEADTLLVDLKPLRKYPLQLPWWLYPLLLALAVAGILIVLLR
                     KPRPTKTPDPEPVAAQPAPPDPAWRMALARLDELRAEGLMLHGHYKLHHYRLSEILRQ
                     FLERKYRFGALEMSTTEINWVTQRIRVDSAAEVIDFLRLCDRVKFAKYVPGFEETANA
                     EEWLRSWLRSFEVEEAQRKLAQAGGGNA"
     gene            complement(1002..1898)
                     /locus_tag="PLB85_06085"
     CDS             complement(1002..1898)
                     /locus_tag="PLB85_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU72934.1"
                     /translation="MPARTPAEILSRVERIEIRARGSVSETFRGEYHSSFRGQGLEFA
                     EVREYQSGDNYRDIDWNVSARLGIPYIKKYQETRELNVFFIVDVSASQNFGTSAAFKQ
                     ERVAEIIALLSLSALSNNDKVGLIMFSEQLEKYLPARKGRNKALEILRDVLYLEPKHP
                     GTNLGEAFTFASRILKKRSVIFILSDLFDSGYQKSLRLLAQKHDVVAVQVLDEAELSL
                     PDAGVLNLRDPETGATLYVNSSHGSVRKAYEAGVKKRQQVLAHELKSMQVDHILIRNR
                     DSAVKALRSFFEERKLRRRTRG"
     gene            complement(1907..>2513)
                     /locus_tag="PLB85_06090"
     CDS             complement(1907..>2513)
                     /locus_tag="PLB85_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOU72935.1"
                     /translation="LLEAMQERQVTIGDTTYPLPKPFFVMATQNPIEQEGTYPLPEAQ
                     IDRFLMKLKIKYPSFEEEKLILDRMMVERKLPVKQVLRPQDLETMRGVIAELHMEDRL
                     KDYILHLIQATRHPQRYPRVSALNGLIQYGASPRATIYLARAAKAHAYLQHRGYVIPD
                     DIKAVGKDILRHRIILSYEAEAEAMNTDEIITRIFEEIEVP"
BASE COUNT          525 a          683 c          720 g          585 t
ORIGIN      
        1 cgcgttttcc tggcgttctg ccagcgggtt tgccacagcc agtatagagg tatcagcagc
       61 aaggctagca accaccaggg atgggcgaat ttaagcattt ccacctcctg cctgggccag
      121 ctttctctgt gcttcctcaa cttcaaagga acgcagccag ctacgcagcc actcttccgc
      181 gttcgcggtc tcctcaaatc cgggaacgta tttggcgaat tttaccctgt cacagaggcg
      241 cagaaaatca ataacttccg ctgccgaatc caccctgatg cgctgtgtga cccagttgat
      301 ctcagtagtg gacatctcca gggcgccaaa gcggtacttg cgttccagaa actgtctcag
      361 gatttctgaa aggcggtagt gatgaagctt gtaatggccg tggagcatca gtccctcagc
      421 gcggagttca tcaagcctgg ctagggccat cctccaagcc ggatcgggcg gggcgggctg
      481 agcggcaacc ggttctgggt ctggtgtttt ggtgggccgg ggtttgcgaa gaagaacgat
      541 aaggattccc gccacagcta gcgccagcaa caacggatag agccaccagg gcagttggag
      601 aggatatttg cgcaaaggtt tcagatccac cagcagcgta tccgcctcag ccctcaccgg
      661 gatgatgttc agccggaagc ggtcggtgaa aagaagctct gaagcggctt cggcggttct
      721 aaccgtcagg gcgggaaagc tatgcgaacc cggatacagc ggcactatcg ttactttcag
      781 ccaatcattg ccgcggggga ctttctgaag ctgccatccc aaaacgtgga atctggtcag
      841 cgtatcgggc accgccacgc tcttgaggtc ggtatcgtgc ctgatttcca gcacaaagcg
      901 gtcgcccacg ctcaggtcat cggcttcaac tagtttctgg ctgagtcctg ccgcggtggt
      961 gagcatcatc cagagcgcga tcagcagtgc caggcacttc ttcatccccg cgttctccga
     1021 cgcagttttc gttcttcaaa gaacgagcgc agcgctttca ccgccgaatc gcggttgcgg
     1081 atgaggatat ggtccacctg cattgatttg agttcatggg caagaacctg ctgtcgtttt
     1141 ttcacacccg cctcataggc tttgcgcaca ctgccgtggg aactgttcac atacaaggtg
     1201 gctccggttt cgggatcgcg cagattcaaa actccagcgt ccggcaggct aagctcggcc
     1261 tcgtccaaca cctgcacggc gaccacatca tgcttttggg ccaataggcg caaggatttc
     1321 tggtagccgg aatcaaacag atcggagagg ataaagataa ccgagcgttt tttgaggatg
     1381 cggctggcga aggtgaaagc ctcgcctaga ttggttcccg ggtgtttcgg ttccagataa
     1441 aggacgtccc tgagaatttc cagcgccttg ttgcgacctt tgcgcgccgg cagatatttc
     1501 tctagttgtt cggaaaacat gattagcccc accttgtcgt tgttcgaaag cgcggaaagg
     1561 gaaagtaggg cgatgatttc ggcaacgcgt tcctgcttga aggcggccga ggtgccgaaa
     1621 ttctgggagg cggaaacgtc cacgatgaaa aacacgttca gttcccgggt ttcctgatat
     1681 ttcttgatgt aagggatgcc cagacgggcg gaaacgttcc agtcaatgtc acggtagtta
     1741 tcaccgcttt ggtattcacg cacttcggca aattccagtc cctgtccccg gaagctggaa
     1801 tggtattcgc cgcggaaagt ttcgctaacg ctaccccggg cgcgaatttc gatccgttcc
     1861 accctggaaa ggatttccgc cggtgtgcgg gctggcatgg ctttccttag ggaacctcta
     1921 tttcctcaaa gatgcgggtg atgatctcat ctgtgttcat ggcttcggct tcggcttcgt
     1981 aagaaagtat gatgcgatgc ctcaaaatgt ctttgcccac ggccttgata tcgtccggga
     2041 tcacgtagcc ccggtgctga agataggcat gcgccttggc cgcgcgggcc agatagatcg
     2101 tggcgcgggg cgaagcgcca tactggatga gcccgttcaa ggctgaaacc cgcggatagc
     2161 gctgcggatg ccgcgtggcc tggatcaggt gcaggatgta gtccttaagc ctgtcttcca
     2221 tgtgcagctc tgcgatcacg ccacgcatgg tttccaggtc ctggggccgc agaacctgtt
     2281 taacaggtag tttgcgctcc accatcatcc ggtccaggat cagtttttct tcctcaaaac
     2341 tgggatattt tatcttcagc ttcatcagaa aacggtcgat ctgcgcttcc ggcaagggat
     2401 aggtgccttc ctgttcgatc gggttctggg tggccatcac gaaaaagggt tttggcaggg
     2461 gataagtggt atcgccgatg gtcacctggc gttcctgcat cgcctccagc aga
//
LOCUS       DAOOWR010000246         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41488_length_2510_cov_3.271283, whole genome shotgun sequence.
ACCESSION   DAOOWR010000246 DAOOWR010000000
VERSION     DAOOWR010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41488_length_2510_cov_3.271283"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1099
                     /locus_tag="PLB85_06095"
     CDS             332..1099
                     /locus_tag="PLB85_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU72936.1"
                     /translation="MIEVQSLVKEFPKKDGTRFRAVDEVSFEAAHGEIVCLLGVNGAG
                     KTTTMRILSTIFQPQSGTARIEGYDVVTQGDMVRQNLGFLSGDTGLYMRLTPREFVTY
                     FGRLYGVGEDDIKAHLNEMAEILDMHDFLDKKMEFLSSGMKQKVSITRSIIHDPPVMI
                     FDEPTAGLDILTARNIISFIRSCKERGKCVLFSTHIMREAERLADRIVMIHQGRVLAQ
                     GTLEQMRSESGFVDLDDIFVFYVNQAGTGTEVAAHEF"
     gene            1089..>2510
                     /locus_tag="PLB85_06100"
     CDS             1089..>2510
                     /locus_tag="PLB85_06100"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOU72937.1"
                     /translation="MNFKKALVIFRKEILEMLRDRRTLFATIVLPVVLYPVLFIGFSA
                     IMSRQTEVLEKRGSTIALVDSLSLRNEASREAYNLILEGLKSTPYLSTLEAPPELEKL
                     YANKDIQAVVSLTDSLASTGTVTYKAGIRFDGSGDQGQMLYSKLEKSLLQTAQTILAK
                     RLSNIKVEQDYIKPLVIRQIDSSSDTKKMGSVLGMVLPYLVILMLVTGASVVAADLVA
                     GEKERRTLETLLVSSATRGEIVLGKYLTIFTMAMINVVINLVSISFSVSYLVSQMDLE
                     TGFVKMPVGSFLILLLAMLPLATLFSALLLSVSTFSRNMKEARTYEQPIMIVSMLMGM
                     VSFIPSVQISNLLALVPVINIALLFKAVLIGEWQLSHLLITVGSTLLLDVFAIWLTVR
                     LFRSEAVLFRTEDDSGGIKTVKTNKRGFFNPFYGLVYYSLALAALYYLGGKWQGQNMV
                     KGLVQTQVFLIGLPVLVLLSILKI"
BASE COUNT          555 a          678 c          684 g          593 t
ORIGIN      
        1 atgcgggcat tattcgagag gtttattggt tgcggaacag aaggccttaa cgaacaatgg
       61 gttgcatgat agggttttgt ccttagattt ttgcaaattg aatagaacat ggatgatgcg
      121 gattgttgtg ttttacggat cagcgggcct gaagctcaga cgtggagagc attgcctgtg
      181 aatgtactat acactgatat aaagcataga tacgatatat tttatggagc atcgcgttaa
      241 cagtcccgac tcggttaagg ttatttaact tgacagggaa gcggcgcgac aaaatcctct
      301 gccgattgaa taataagcat aaggtgataa tatgatcgag gtccagtcat tggtcaagga
      361 attccccaag aaggacggaa cccgcttccg ggctgtggat gaggttagtt ttgaggccgc
      421 ccacggtgaa atcgtttgcc ttttgggggt gaacggcgct ggcaagacca ccacgatgcg
      481 catcctctcc accatcttcc agccccagag cggaaccgcg cggatcgagg gttacgacgt
      541 ggttacccag ggtgacatgg ttcgtcagaa cctgggtttt ctctccggcg acaccggttt
      601 gtacatgcgc ctcactccac gggagtttgt gacctatttt ggccgcctct atggagtcgg
      661 ggaagacgac atcaaggccc atttgaacga aatggcggaa atcttggaca tgcacgattt
      721 cctggacaag aagatggaat tcctctccag cggcatgaag caaaaggttt ccatcacgcg
      781 ctccatcatc catgatccgc cggtgatgat tttcgacgaa ccaaccgcgg gcctggacat
      841 cctcacggca cgaaacatca tctcgttcat ccgcagctgc aaggaacggg gcaaatgcgt
      901 gcttttttca acccacatca tgagagaagc ggagcgcctg gcagaccgta tcgtgatgat
      961 ccaccagggc cgcgtgctgg cgcagggcac cctggagcag atgcgctcgg aaagcggttt
     1021 tgtcgatctg gacgatatct ttgttttcta cgtgaaccaa gccggaaccg gcacggaggt
     1081 ggctgcccat gaattttaag aaagccctcg tcatcttccg caaagagata ctggaaatgc
     1141 tgcgcgaccg gcgcaccctc tttgccacca tcgttttgcc cgtggttctt tatcccgtgc
     1201 tgttcatagg cttcagcgcc atcatgagcc gccagaccga agttctggaa aagcggggct
     1261 ccacaatcgc cctggtggac agcctgagcc tccgaaacga agcttcccgc gaggcctaca
     1321 acctcattct ggaggggttg aagtcaactc cctacctcag cactctggaa gcgccgcccg
     1381 agctggaaaa actctacgcc aacaaggaca tccaggccgt cgtgagcttg accgattcgc
     1441 tggcttccac cggaaccgtg acttataaag ccggcatccg cttcgacggt tcaggtgatc
     1501 agggccagat gctttacagc aagctggaaa aatcgctgct gcaaaccgcc cagaccatcc
     1561 ttgccaaacg cctcagcaac atcaaggtgg agcaggacta tatcaagcct ctggtaatca
     1621 ggcaaataga cagttcctcc gacaccaaga aaatgggcag cgtgctgggt atggtgctcc
     1681 cctacctcgt tatcctgatg ctggttaccg gggctagtgt ggttgccgcg gaccttgtgg
     1741 ccggggaaaa ggaacgccgc accctggaaa ccctgctggt gtcttccgcc acccgcggag
     1801 agatagtttt gggcaaatac ctcaccatct ttaccatggc catgatcaac gtggtgatca
     1861 acctggtgag catcagtttt tccgtgagct atctggtcag ccagatggac ttggagactg
     1921 gatttgtgaa gatgcccgtt ggttcctttc tgatcctgct gctggcgatg ttgcccctgg
     1981 ccacactgtt ttccgctttg ctgctttccg tctccacttt ctcgcgcaac atgaaggaag
     2041 cccgaaccta cgagcagcct atcatgatag tttccatgct gatgggcatg gtgagcttca
     2101 tcccctccgt ccagatcagc aacttgcttg ccctggtgcc ggtaatcaac atcgccttgc
     2161 tcttcaaggc tgtgctgatc ggcgagtggc agctatccca cctgctgatc acggtgggtt
     2221 ctaccctgct tttggacgtt tttgccatct ggctcaccgt gcgcctgttc cgttccgaag
     2281 cggtgctttt ccgcaccgag gatgacagcg gcggcatcaa aacagttaag acgaacaagc
     2341 gcggcttctt caaccccttc tatgggctgg tctattactc gctggccctg gccgcgctct
     2401 attatctggg tggcaagtgg caggggcaga acatggtgaa aggcttggtg cagacccagg
     2461 tattcttgat cgggctgccg gtgctggtgc tgctgagcat cctgaagatc
//
LOCUS       DAOOWR010000247         2500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41718_length_2500_cov_3.602863, whole genome shotgun sequence.
ACCESSION   DAOOWR010000247 DAOOWR010000000
VERSION     DAOOWR010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41718_length_2500_cov_3.602863"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PLB85_06105"
     CDS             <1..1558
                     /locus_tag="PLB85_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72938.1"
                     /translation="VATSATNNIWRVPSWRSGEYYLLEYRRPSENYDHNVPGTGLLVY
                     RLDSSESGNASGPPDELYIYRPNANNTTTNGTLNNAEFSLQVGRTEMNEATVPSGFMS
                     NNSPGGLNLYDVGEAGETITFKIKISDVQLTFPRGGETWFSGSNKVLTWKAKSMTGTV
                     TVDYSTDGGVNWTVLNADAPNNGSYAWNNIPVLNSNQVFVRITQNSSGQADSNIHPLA
                     IASEMATPQGTWPPDGATEIPTNPLLAWTATPGVTGYQLQVSTDPNFNSYLVNAIDHP
                     DNHYQLQGLTPFQTYYWRVCGLADIGPGPFCLDLSFTTGDITELPDPPVLTYPVHQAE
                     DLPLELDFSWTPMPLADSYSLQISQSFWFVDPEIDLPNLTTTSQHVSGLQPGRTYFWR
                     VSAGNVAGNSNFSHINQFTTLQSTPVEDDTAPVVINELKQNYPNPFNPTTTISLSLKD
                     ASLPASLQIFDLRGRLVRTLFQGIPSGQRMELVWDGKDSQGRACGSGIYHYRLRSGDF
                     VQTRKMILMK"
     gene            1687..2124
                     /locus_tag="PLB85_06110"
     CDS             1687..2124
                     /locus_tag="PLB85_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU72939.1"
                     /translation="MKITPYRKGQELRPMGTMLSIFDDFFNRAFEEESSEDNFRSMAM
                     DIVEHEKEFELLANLPGFKKENVKISVHDNQLMIEAKCEQSKEETKGTVYRCERYSGS
                     YRRNLLLPENSDLAKISAKMEDGVLKLIIPKKEPSPQKEIVID"
     gene            complement(2193..>2500)
                     /locus_tag="PLB85_06115"
     CDS             complement(2193..>2500)
                     /locus_tag="PLB85_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU72940.1"
                     /translation="CYADRDGREIKAQRKATAWPADETNAKLKVQFFWPIKADYWIVK
                     LDQAKYSYTVVSEPTREHLWILTRDKAIDKVLYDEIVNWLKDNGWDTGKLVFTGSFK"
BASE COUNT          607 a          732 c          630 g          531 t
ORIGIN      
        1 ggtggctacc tccgccacaa acaacatctg gcgcgtgccc agctggagaa gcggcgaata
       61 ctatctgctg gaataccggc gcccttccga gaattacgat cacaacgtcc ccggcaccgg
      121 cctcctggtt tacaggcttg attcctccga aagcggcaat gccagcgggc cccctgacga
      181 gctctacatc tatcgcccca atgccaataa caccaccacc aacggcaccc tcaacaatgc
      241 tgagttttcg ctccaggtag ggcgaaccga gatgaacgaa gccacggttc ccagcggctt
      301 catgagcaac aactccccgg gcgggctgaa tctctatgat gtgggcgaag cgggtgaaac
      361 catcaccttt aaaattaaga tttcggatgt ccagctcacc tttccccggg gcggcgagac
      421 ctggttttcc ggcagcaaca aggtgctaac ctggaaagcc aaaagcatga ccggaaccgt
      481 aaccgtggat tacagcaccg atggcggcgt gaattggacg gtattgaatg ccgacgcgcc
      541 caacaacggc agctatgcct ggaacaatat ccccgtgctc aattccaatc aggttttcgt
      601 gcgcatcacc cagaactcca gcggccaggc ggacagcaac atccatcctt tagcgattgc
      661 cagcgaaatg gccacgccgc aggggacctg gccacccgac ggcgccacgg aaattcccac
      721 caacccgctg ctggcctgga ctgccacgcc gggagtgacc ggatatcaat tgcaggtttc
      781 aaccgacccc aacttcaaca gctacctggt gaacgccatt gaccatccgg acaaccacta
      841 tcagttgcag ggccttacgc ctttccaaac ttactattgg cgggtctgcg ggcttgccga
      901 catcggaccc gggcccttct gtctcgacct cagcttcacc accggcgaca tcaccgaact
      961 gcctgaccca ccagtgctta catatccggt acatcaggcg gaagacctgc ccctggaact
     1021 cgatttttcc tggactccca tgcctctggc agatagttac tctctccaga tttcgcagag
     1081 cttttggttc gtggatcctg aaattgactt gcccaacctt accaccacca gccagcatgt
     1141 ttccggcctg cagccagggc gcacctactt ttggagggtg agcgcgggga acgtggccgg
     1201 aaacagcaac ttcagccaca tcaaccagtt taccactctc caatccactc ccgtggaaga
     1261 cgatacggct ccggttgtga ttaacgagct gaaacagaat taccccaatc ccttcaatcc
     1321 cacaaccacc atcagcctta gcctgaaaga cgcctccctg cccgcctctt tgcagatttt
     1381 cgacctccgc ggcaggctgg ttcgcaccct tttccaggga atcccttccg gacagcgaat
     1441 ggagctggtc tgggacggca aggacagcca gggcagagct tgcggcagcg ggatttatca
     1501 ttatcgcctg cgttccgggg atttcgtcca aactcgtaag atgatattaa tgaagtaaat
     1561 aagcccgcct atcggcggac ccggaaaaca aaattagcag ttccaccacg ggactgctaa
     1621 ttttttcttg cctttttggg ttatgtgatt atattgactg tagagattca ttaaaggagg
     1681 tttgaaatga agatcacacc ttatcgcaaa ggacaagaac ttagacccat gggcaccatg
     1741 ctcagtatat tcgacgattt cttcaaccgc gctttcgaag aggagagcag cgaggacaat
     1801 ttccgctcca tggcgatgga tatcgtggag cacgaaaagg aatttgagct tctggccaat
     1861 ctgcccggct tcaaaaaaga aaacgtgaag atctcggtgc acgacaacca gttgatgatc
     1921 gaggccaaat gcgagcagag caaagaggaa accaaaggca ccgtgtaccg ctgcgaacgc
     1981 tatagcggca gctaccggcg caacttgctt ctgcccgaga attccgatct ggcaaaaatc
     2041 tcagccaaga tggaagacgg cgtactgaag ctgatcattc ccaagaaaga accctcaccg
     2101 cagaaagaaa tcgttatcga ttaagcaaat gtccaactaa ataggcggcg cgtaccatga
     2161 ggtgcgcgcc gcctatttag tattgggaaa acttacttga agcttccggt gaagacaagc
     2221 ttgccggtat cccagccatt gtctttcagc cagttcacga tttcgtcgta aagaaccttg
     2281 tcgatggctt tgtcgcgggt aaggatccac aggtgttcgc gggtgggttc actgaccacg
     2341 gtgtagctgt atttggcctg atcgagcttt acgatccagt aatccgcctt gatgggccag
     2401 aaaaactgga ctttcagctt ggcgttggtc tcgtccgcag gccaggctgt ggctttgcgc
     2461 tgggccttga tttccctgcc gtccctgtcg gcgtagcagg
//
LOCUS       DAOOWR010000248         2498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41760_length_2498_cov_4.268113, whole genome shotgun sequence.
ACCESSION   DAOOWR010000248 DAOOWR010000000
VERSION     DAOOWR010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41760_length_2498_cov_4.268113"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..>2498
                     /locus_tag="PLB85_06120"
     CDS             221..>2498
                     /locus_tag="PLB85_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72941.1"
                     /translation="MKKQCFVIMALLLFVMCLGAQEVALRKAWKMDFRGIQVRTADNC
                     VICIWEDTDAGDTDIYAQKFNHSGVAVWTQPQIIVGEPGVQEAVACVPTSDNNFVLLY
                     QQSGHGYEAGVWLQKFSSNGQCLWGESGVCIRNGSAYLANACLVPNAVGGVFALYPHS
                     SNGYAVIGQNLDSFGNQLWPSGGLTLASNPYFLSLDGAVSDGEGGIIIDVTPYQGINT
                     LTRFSPQGTVVGNNPLLPAGVFPGGQFSIVEDGNGNYLLYGYRNGALILQRMNNQGEL
                     LLPAAVQTHLQIGYTDFNPPRLEPLADGGLIFAWEVLTNDDTRELRAIRLDSNLNSLW
                     GPSGVQIASGVSQNWFYMGLDLSVTDSGEAWLTWYESLSGEVPHTLKAQYLSPTGTVP
                     WDVGGIAINAGDPMLGKPVALAFPDRGMFLWADQRDSYESLRLQVLSTGGAAFQDLGG
                     EAQVTRLVGLANMSEVIALQDRYLVLWTDLRNSYYQIYYQICDTNMNPLLEPDGRPLC
                     PSLQLHLTNIRTVRMLDNSVAILLYGANCDGSQWYPLHLQRIDAEGNTMYPGHGLQVN
                     DNYSIQDTRISACGNDIYVAWTEGGYPQYHLMGQKISNGQIQWGPSGKALYTPPQQTW
                     LGVADLVENYVVFNTIDYSNDYSPARVLRIDPNGDPASGWPAEGVAVVSTEASIDSMN
                     PGTALLGNDLVVFCTTHDQDYQNYSFLAQKIGPDGSRLWSETGIELPFTGIHSMAVSD
                     NAIDILYSSAWDPGIQDLR"
BASE COUNT          566 a          736 c          659 g          537 t
ORIGIN      
        1 agcgcaacct tgccaaggtt gatgtcgcgg gttcgagtcc cgtttcccgc tccatatttt
       61 cctaaagaat gatcactact cccctgccag gccctgaatg ggtttcaccg tactccaact
      121 aaccgaacct aagaaacata aaagcttatc gccaacggtt tggaacaaaa cttgcaatct
      181 gcatcccata agtcaatcaa ttgctaaaag ggagacagaa atgaaaaagc agtgtttcgt
      241 gatcatggca ttgttgcttt ttgtaatgtg cctcggcgcg caggaagtcg ccctgcgcaa
      301 agcctggaag atggacttca ggggaataca ggtccgcaca gcagacaatt gcgtgatctg
      361 catctgggaa gacaccgatg ccggcgatac cgacatctac gcgcaaaagt tcaatcactc
      421 gggagtggcg gtgtggacac agccacagat catagtgggc gagcccggag tgcaggaagc
      481 agtggcctgc gtgcccacct ccgataacaa tttcgtgctg ctctaccagc aatccggaca
      541 cggctacgaa gccggtgtct ggctgcagaa attcagttcc aacggccagt gtctctgggg
      601 tgaaagcgga gtgtgcatcc gcaacggctc tgcctatctg gctaacgcct gtctggtccc
      661 caacgccgtg ggcggagttt ttgccctcta cccccattcc tcaaacggtt acgcggtgat
      721 cggccaaaac ctggatagct tcggcaatca gctttggccc agcggcggac tcactctggc
      781 cagcaatcct tactttctca gcctcgatgg tgctgtcagc gatggcgagg gcggcatcat
      841 aatcgacgta actccctatc agggaatcaa taccctcacc cgcttttccc cacagggcac
      901 ggtggttggc aacaatcccc tgcttcccgc aggcgtgttt cccggcggcc agttctccat
      961 tgtggaggac ggcaatggca attacctgct ctacggatac cgaaacggag cccttatcct
     1021 gcagcggatg aacaaccagg gcgaactgct cttaccagcg gccgtgcaaa cccatctcca
     1081 gatcggctat accgatttca atcctccccg cctggaaccg cttgctgatg gtggcctcat
     1141 cttcgcttgg gaagttctga ccaatgacga cactagggaa ctgcgggcta tccgtctgga
     1201 ttcaaacctc aactcactct ggggccccag tggtgtacaa attgccagtg gagtttccca
     1261 aaactggttc tacatgggac tggacctctc cgtaactgac agcggcgagg cctggctcac
     1321 ctggtacgaa tccctctccg gcgaagtgcc ccacaccctc aaagcccagt atctgtcacc
     1381 aaccggaacc gttccctggg acgtgggcgg aattgccatc aacgccggcg accccatgct
     1441 cggcaagcct gtagcactgg ctttcccgga ccgggggatg tttctctggg cggaccagcg
     1501 cgattcctat gaatccctgc ggctgcaggt gctcagcact ggcggagccg ctttccagga
     1561 cctcggcgga gaagcgcagg tgacccgttt ggtcggcctg gccaatatgt ccgaggtgat
     1621 tgccctgcag gacaggtatc tggtgctgtg gacagacctc cgcaacagct actatcagat
     1681 ctattaccag atctgtgaca caaacatgaa tcccctgcta gaaccggatg gccggccgct
     1741 ctgcccttcg ttgcagttac acttgaccaa tattcgcacg gtgagaatgc tggacaatag
     1801 cgtggcgata ctactgtacg gtgccaactg tgatggttcc caatggtatc cgctgcacct
     1861 gcagaggatc gatgccgagg gaaataccat gtatcccggc catggcttgc aggtcaacga
     1921 caactacagc atccaagaca cccgcatcag cgcttgtggt aatgacattt acgtggcctg
     1981 gacagaaggc ggctatcccc agtatcattt gatggggcaa aagatatcca acggacaaat
     2041 ccaatggggg ccttcaggaa aagcccttta cacccctccc cagcagacgt ggttgggcgt
     2101 ggctgacctt gttgaaaact acgtggtctt caatactata gattacagca atgactattc
     2161 tcctgcccgg gttctgagaa tagatcccaa cggcgatccc gcctctggct ggcctgctga
     2221 gggggtcgcg gtggtaagca cagaagcttc catcgacagc atgaatcccg gaacagccct
     2281 gctgggcaac gatctggtgg tattttgcac cactcatgac caggattatc aaaactacag
     2341 cttcctggcc cagaagatcg gcccggacgg atccaggctc tggtctgaaa ccggcatcga
     2401 gctccccttc accggaatcc acagcatggc ggtctcggat aacgccattg acattcttta
     2461 ttcttcggca tgggatccgg ggattcagga cctgcgcc
//
LOCUS       DAOOWR010000249         2492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41902_length_2492_cov_3.990973, whole genome shotgun sequence.
ACCESSION   DAOOWR010000249 DAOOWR010000000
VERSION     DAOOWR010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41902_length_2492_cov_3.990973"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLB85_06125"
     CDS             complement(<1..936)
                     /locus_tag="PLB85_06125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOU72942.1"
                     /translation="MDSFIMHNPTRVHFGVGTIERIGPELQSAGVRKCLLVAGGGSIR
                     ANSVYEQVSKSLRGSRIDWTEAWGVQANPTLEKVYELIALAKTEQVDAILAVGGGSVI
                     DSAKATAAGFYLRDVWNAFTGVERIEQALPVFTVLTLSATGSEMNGNAVITHTEEQKK
                     WSIGSPLLYPRVSIIDPSVQSSLPWRQTVNGALDATAHILEYFFMDSPVETTRAINVA
                     LLKTITSATNRLQQNPADLVARGNLAWCATLALNGISGAGLQGGDWSCHSIEHAFSVL
                     DPRIAHGEGLGVIFPAWIEYVSERQPTRFEYWAKNV"
     gene            1129..1269
                     /locus_tag="PLB85_06130"
     CDS             1129..1269
                     /locus_tag="PLB85_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72943.1"
                     /translation="MHRIVCLAVLLLSYNIYRRAGNGETLLASTWVWNQGLMPNSNGS
                     YS"
     gene            1603..>2492
                     /locus_tag="PLB85_06135"
     CDS             1603..>2492
                     /locus_tag="PLB85_06135"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOU72944.1"
                     /translation="MLLIVSPSHWAPFNIASEEHILQNYAENVFLLYRNAPSIIVGRN
                     QNALAEINLDWVTRHKIPVVRRLTGGGTVFHDMGNLNFSFIMNRSAREESGFAKYTAP
                     VLAVLRNLGVDAKLEGRNDLTIKGMKFSGNARAVYKNKVQQHGTILFSSHIADLSAAL
                     KANPLKFSDKAVKSVSSRVTNVCEHLPKPLPLEELIALVQAEVLGMYPEARAHSFSPD
                     DEAAIQALAKDKYDCWDWNFSRSPAYNRVQAIRCAAGTIEFHADVHKGRITALKVYGD
                     FFGNRDIAGFEAALIGVTHR"
BASE COUNT          585 a          715 c          669 g          523 t
ORIGIN      
        1 cacgttttta gcccaatatt cgaaacgggt gggctgtctt tcgcttacat actcaatcca
       61 ggcgggaaaa atcactccga ggccctctcc gtgggcgatg cgggggtcca gaacggaaaa
      121 agcgtgctcg atgctgtggc aggaccagtc accgccctga aggcctgcgc cgctgatgcc
      181 gttgagagcc agggtggcgc accaggccag gtttccgcgg gccacaaggt cggcgggatt
      241 ctgctgcagc ctgttggtgg ctgaagtgat ggttttaagc agggccacat tgatggcgcg
      301 tgtagtttct accgggctgt ccatgaaaaa gtattccaga atatgtgctg ttgcatccag
      361 ggctccgttc accgtctggc gccagggcag gctgctttgc acagagggat cgatgatgga
      421 gacgcgcgga tagagcagcg gtgagccaat gctccacttc ttttgctctt cagtgtgggt
      481 gatcacggcg ttgccgttca tctcgctgcc agtggcggaa agcgtgagca ccgtgaagac
      541 gggcaaagcc tgttctatcc tttccactcc ggtgaatgcg ttccacacat cccgcaggta
      601 aaaacctgcc gcggtggctt tggcactatc gataacgctg cctcctccca cggccagaat
      661 ggcgtccact tgctcggttt tggcaagggc aatcagctca tacacctttt ccagagtggg
      721 attggcctgc acaccccaag cctcggtcca gtcgatccgg ctgccccgga gacttttact
      781 gacctgttcg tacacgctat tcgcgcgaat cgagcctcca ccagccacca gcaggcattt
      841 gcgcactccg gcactctgaa gttctggtcc gatccgctct atcgttccca ccccaaaatg
      901 gacccgggtg gggttgtgca taatgaagga atccatttct gctccttaat cttgtaaact
      961 gattcaaaag ccttgtcggt tcaagaatat attaaaggca aatcgctgcc gggcaagctg
     1021 aaatgcgctt caccgccaag cgaatcctct tcaaaacata tttcttgaca gccccggcat
     1081 taagaaagaa aaagtaaaaa gaagaaagca tccctgcagg agacatccat gcatcgtatc
     1141 gtctgcctcg ctgttctgct gctgagctac aacatctacc gccgcgctgg gaacggtgag
     1201 actctgcttg cctcaacctg ggtatggaac cagggcctga tgcctaattc gaacggctct
     1261 tacagctaat aggacacggg gctgaccaat gggatggtct tgagcgctaa gggcccggca
     1321 gacaggacag cgcggcgggt aaggagttat ggctctctat tgaatcaagt ggctgtggat
     1381 attcatactg aaaacagacc gaggggcttt accagccata ttggagcctt agcctaaaac
     1441 actgatcgcc attgacaaca agcgcccggc aaaaatcatg gtcagtgata agcgactaat
     1501 cacaagaaag atgaatgcaa cttccactgc cagaaaacaa tacctgatga aaacgaatct
     1561 ggatgagttt ccccaacctc cagccataac accaggccgg ctatgctgct gatcgtaagc
     1621 cccagccact gggcgccgtt caacatcgcc agcgaggaac atatcctcca aaactacgcg
     1681 gagaacgtat tcctgctcta ccgcaacgcg ccttccatca ttgtgggccg caaccagaac
     1741 gcactggcgg agatcaactt ggattgggta acccggcaca agattcccgt ggtgcggcgc
     1801 ctcaccggtg gcgggaccgt ttttcacgac atgggtaacc ttaacttcag cttcataatg
     1861 aaccgctcgg ccagggagga aagcggattt gccaaataca ccgcgccggt gctggctgtc
     1921 cttaggaatt tgggtgtgga tgccaagctg gagggacgca acgacctcac catcaaaggg
     1981 atgaagttct ccggcaacgc ccgcgctgtt tacaaaaaca aggtccagca gcatggcacc
     2041 atcctctttt ccagccacat tgccgacctc agcgcagccc tgaaagccaa tccactcaaa
     2101 ttcagcgaca aagctgtgaa atcggtgtcc agccgcgtga ccaatgtgtg cgaacatctg
     2161 cccaagccat tgccgctgga ggagctcatc gccctggtgc aggctgaggt gctggggatg
     2221 tatccagaag cccgggccca ttccttcagc cctgatgacg aagccgccat ccaggctttg
     2281 gccaaggaca aatatgactg ctgggattgg aatttcagcc gctccccagc ttacaaccgc
     2341 gtgcaggcaa tccgctgcgc cgccggcacc atcgagttcc acgccgacgt ccacaaaggc
     2401 cggatcacgg ctttgaaggt gtatggcgat ttcttcggaa accgcgatat agcgggattt
     2461 gaagccgcgc tcatcggcgt tacccaccgt ga
//
LOCUS       DAOOWR010000250         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_41982_length_2489_cov_4.152835, whole genome shotgun sequence.
ACCESSION   DAOOWR010000250 DAOOWR010000000
VERSION     DAOOWR010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41982_length_2489_cov_4.152835"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="PLB85_06140"
     CDS             complement(<1..1213)
                     /locus_tag="PLB85_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU72945.1"
                     /translation="MKKQAVKHGFAHIASREIPEIAATAHYYEHEKSGARLVHMACED
                     NNKVFCATFKTVPTDNTGCPHILEHSVLNGSKNFPCKSTFQELIKGSLHTFINAMTDS
                     DMTLYPVASTNAKDFVNLSRVYLDAVFFPKIYEQPNILHQEGWHYELTDPEGELKVRG
                     VVYNEMKGAFSSPDQVIERYSQQAQFPDNTYGCESGGDPKEIPQLTYENFIAFHKKHY
                     HPSNSHLFLYGDMDADAMMEIMDWEYLSHFEKDTEAVNIPLQKPFDRMKKLEVQYPVE
                     EGKDITEQYHLSLNYTFGLNPDLKLTHAITVLMSILMGTPASPLKQAILKSGLAKDTI
                     FSLREGLLQPTFSIICKQVRRENADALASLIRTELKRLVEDGIDKKLVEAVLNRREFA
                     LREAQMSWGPRG"
     gene            complement(1230..2105)
                     /gene="dapA"
                     /locus_tag="PLB85_06145"
     CDS             complement(1230..2105)
                     /gene="dapA"
                     /locus_tag="PLB85_06145"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU72946.1"
                     /translation="MLQGSYVALVTPFKNGGIDYFALEALLEFHIAEGTHGILLLGTT
                     AETAALASDEKEALLLFALQKINKRVPVMIGTGTNNYNQTLAATKRAKELGADHALVI
                     TPYYIKPTQNGLYGYYKALSTKVDIPIVVYNVPGRTGVNISAATMVKLASECPNIVGV
                     KEASGNIPQATEIIRDAPETFSLMSGEDALNLPLMAIGGKGCISVTANVVPKLMSEHI
                     SSCLTGDLATAAKQHRHLQKLNGAMFIETNPIPAKEALHMMGMIELEFRLPICPLQDA
                     NREVLRAVLKEYDLT"
BASE COUNT          476 a          677 c          710 g          626 t
ORIGIN      
        1 gccctctcgg cccccaggac atctgagcct cacgcagagc gaactcgcgc cggttgagta
       61 cggcctcgac cagttttttg tcaatgccat cctccaccag gcgtttcagc tcggtccgga
      121 tcaggctggc cagcgcgtcc gcgttttccc gcctcacctg tttgcagatg atgctgaaag
      181 ttggctgcag caggccttcc cgcagggaaa agatggtgtc cttagccagg ccggatttga
      241 gaatggcttg tttcagcggc gaggccgggg ttcccatcag aatgctcatc agcaccgtga
      301 tcgcgtgggt tagcttgagg tcgggattaa ggccgaaagt gtagttcagg ctcaggtgat
      361 actgttccgt gatgtccttg ccctcttcca ccggatactg cacctccagc ttcttcatcc
      421 tgtcaaaggg cttttgcagc gggatattca ccgcttccgt gtctttttcg aaatggctca
      481 ggtattccca gtccattatt tccatcatcg cgtcggcgtc catgtcccca tagaggaaga
      541 gatgcgagtt cgagggatgg tagtgttttt tatggaaggc gatgaagttc tcgtatgtta
      601 gctgcggaat ttcctttggg tcgcctccgg attcgcagcc gtaggtgtta tccggaaact
      661 gcgcctgctg gctgtagcgt tcgataacct ggtccggcga ggaaaaagcg cccttcatct
      721 cgttgtaaac cacgcctctg accttcagct cgccttccgg gtcggtgagt tcatagtgcc
      781 agccttcctg gtgcaggatg ttgggctgtt cgtatatctt ggggaaaaac accgcgtcca
      841 agtaaacccg gctgaggttc acgaaatcct tggcgttggt gctggcaacg ggatacagcg
      901 tcatgtccga atcggtcatg gcgttgatga aggtgtgcag cgaacccttt atcagttcct
      961 ggaaggtgga tttgcaggga aagttcttgg agccgttaag caccgaatgt tccagtatgt
     1021 ggggacagcc ggtgttgtcg gtgggaacgg ttttgaaggt ggcgcagaaa accttgttgt
     1081 tgtcctcgca cgccatatgc accaggcgcg ccccgctttt ctcatgttcg taataatgtg
     1141 ccgtggcggc gatttcaggt atctcgcggc tggcaatgtg ggcaaagccg tgtttcacag
     1201 cctgcttttt catggttgaa ctcctaatat caggttaaat cgtattcttt cagaacagcg
     1261 cgcaaaacct cgcggttggc gtcctgcagc ggacagatcg gcagccggaa ttccagttcg
     1321 atcatcccca tcatatgcag ggcttccttg gccgggatgg gattggtctc tatgaacatc
     1381 gcgccattca gcttctgcaa gtgccggtgc tgtttggcgg cggtggcgag atccccagtc
     1441 aggcagctgc tgatgtgctc gctcatcagt ttgggcacaa cgtttgccgt cacggaaatg
     1501 cagcctttgc cgccaatggc catcagcgga aggttgagcg cgtcctctcc gctcatcagg
     1561 gaaaaagttt ccggagcgtc gcggattatc tccgtcgcct gggggatatt gccgctcgct
     1621 tccttgacgc cgactatatt tgggcattcg cttgccagct tcaccattgt cgccgcggaa
     1681 atattcaccc cggtgcggcc gggaacgttg taaaccacga ttgggatgtc cactttggtg
     1741 gacagggcct tgtaatatcc atacaggccg ttttgcgtgg gcttgatgta atagggcgtg
     1801 atcaccaggg cgtggtctgc ccccagctct ttcgcccgct tggtggcagc cagggtctgg
     1861 ttgtagttgt tcgtgcccgt gccgatcatc accggaaccc gtttgttgat cttctgcagg
     1921 gcgaaaagca gcagcgcctc cttttcgtcc gaagccaggg cggcggtttc agcggttgtg
     1981 cccagcagca ggatgccgtg ggtgccctcg gcgatatgga actccaacag ggcttccagg
     2041 gcgaaatagt cgatcccgcc attcttgaag ggcgtcacca aagccacata agaaccttgc
     2101 aacatgtttc tatctccttt tcccaatgtg ggttggctgc gccggaccaa atccctcccc
     2161 gggggagggc gggtttggcg cagaaaaaca caattttgac cactctgccg cgctcccaat
     2221 ttccgtcaat acaaatatgc gccaagcctc tgacgccctg tggaatttca tcccgttagc
     2281 aaatgacctg ctcccgcttt cctgaagtaa cagacaaaat tccagagcgg acgcggacag
     2341 cctctttcct tatctcgact cagcctggcg tacagtggac gcagtggaca tggacgacag
     2401 aatgcttgtg aactttaatt ctttgctacc gtcgatgctg ttggagcctg gagtccagcg
     2461 agcctgcgag cttggactac aggaagtgt
//
LOCUS       DAOOWR010000251         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_42091_length_2484_cov_2.960889, whole genome shotgun sequence.
ACCESSION   DAOOWR010000251 DAOOWR010000000
VERSION     DAOOWR010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42091_length_2484_cov_2.960889"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLB85_06150"
     CDS             <1..240
                     /locus_tag="PLB85_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72947.1"
                     /translation="SYQTSPDIPILVQTQSQLAAETGSAFWNLYQAMGGYNSMIGYVN
                     ASPRLAAKDYTHFTRAGADKLARMLHKVLTTGKND"
     gene            233..1201
                     /locus_tag="PLB85_06155"
     CDS             233..1201
                     /locus_tag="PLB85_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOU72948.1"
                     /translation="MTELGHKLLLLLLLCLLFTTLVWADESDDPDSQSTPASVVLTED
                     MPEYPEAWPSEELPEGIDPLDEYIHSHFALRAVEGYFHGLNFLKRDANLIPATDTLLA
                     PFLEKLMELRSGRRKQVTLFQFGGSHIKPGWFAGATEKEFNRWLAESAEEGFSPTLKF
                     HYKGINGASFRNQLNNQEIFDLCRNLKPDLIVISMGTNDAQGTYSATRFRTAIDNFMT
                     KLYKYSGDALILFTLPPDSYKNGVPNADVAKVGAEIAAYAKAHGHVCWDLQTVMGGQG
                     SMSKWRSGGIAGKDMVHFSAPGYRLQGQLFFEALMRAYKHYAENRP"
     gene            1198..>2484
                     /locus_tag="PLB85_06160"
     CDS             1198..>2484
                     /locus_tag="PLB85_06160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOU72949.1"
                     /translation="MILERILQSLVYDPKNPLIFNSSFFLFFFAVVMLFYPFVVKKIK
                     VRTWYLMLASLYFYYKTSGVFVLLLVITAGINYLIGFGLENSKSQGRRRLLIALSVIW
                     NLGSLGYYKYSNFLIDTINGLFGGNLPALDIFLPVGISFFTFQTMSYTLDIFYRKLKP
                     VNSFADFLFFVSFFPQLVAGPILRASWFIPQINKQLSLNKEQVAKALLLIFTGLVKKG
                     VIADYISINFVDRVFDNPALYSGLENLLALYGYSLQVYCDFSGYSDIAIGLATMMGFE
                     LQPNFNSPYRASSLGDFWRRWHISLSTWLRDYLYIPLGGSRKGKVRTYVNLMITMVLG
                     GLWHGASWNFVLWGALNGVGLCIDRFVHNIFWPRSKDKSAPPRQTPLLLKILGILFTF
                     NFITFTRIFFRSRTFDDALTMMERIFTNLNPRLLFIW"
BASE COUNT          542 a          763 c          575 g          604 t
ORIGIN      
        1 tcttaccaga cctcgccgga cattcccatc ctggtgcaaa cccaaagcca gctcgcagct
       61 gagaccggct ccgctttctg gaacctatat caagctatgg gcggctacaa ttccatgata
      121 ggatacgtga acgcaagtcc caggcttgcc gccaaggatt acacccactt caccagggcc
      181 ggtgccgaca aactggcccg gatgctccac aaggtgctca ccaccggcaa aaatgactga
      241 gcttgggcac aaattgctgt tgctgcttct tctctgccta ctttttacaa ccttggtttg
      301 ggctgacgaa tctgatgacc cagattccca gtcaacccct gcctcagttg tgttgacaga
      361 ggatatgccg gaatatccgg aagcctggcc aagtgaggaa ctgccggagg gaattgaccc
      421 cctggacgag tatatccaca gccattttgc cctgcgagcc gttgaaggtt atttccacgg
      481 tttgaacttc ctgaaaaggg acgccaacct catccctgcc acggataccc ttctggcgcc
      541 atttctcgag aagctgatgg aactgcgttc aggacgtagg aaacaggtca ccctcttcca
      601 gtttggcggc tcccacatca agccaggctg gttcgccgga gccacggaaa aggagttcaa
      661 ccggtggctg gcggaatcag cggaagaggg cttctccccc actctaaagt tccattacaa
      721 gggcatcaac ggagcctcct tccgcaacca gctcaacaat caggaaatct ttgacctctg
      781 ccggaacctc aaacccgacc tcatagtcat ttccatgggc accaacgacg cccagggaac
      841 ttacagcgcc acccgtttcc gcacagccat agacaatttc atgactaaac tctacaaata
      901 cagcggcgac gccctcatcc tcttcaccct gcccccggat tcctataaaa acggagtccc
      961 aaacgccgat gtggccaagg ttggggcaga aatcgccgct tatgccaaag cccacggcca
     1021 tgtttgctgg gatctccaga cggtgatggg cggccagggc tccatgagca aatggcgctc
     1081 cggtggaatt gccggcaaag acatggtgca tttcagcgcc ccgggctacc gtctgcaggg
     1141 gcagcttttt tttgaagccc tcatgcgggc ttacaaacat tacgcggaga accgtccttg
     1201 atcctcgaaa gaatattgca aagcctggtt tacgatccca agaatccgtt gatcttcaat
     1261 agtagcttct tcctcttttt ctttgccgtc gtgatgctct tctacccctt tgtggtgaag
     1321 aaaatcaagg ttcgcacctg gtatttgatg ctggcctcgc tctacttcta ttacaagacc
     1381 agcggcgtct ttgtgctgct gcttgtgatc accgccggca tcaactacct aatcggtttt
     1441 ggcctggaaa actccaaaag ccagggccgg cgaaggctgc ttatcgcact cagcgtgatt
     1501 tggaacctcg gttccctggg ctattacaag tattccaatt tcctcatcga tacgataaac
     1561 ggccttttcg gaggcaatct gcccgccctg gatattttcc tgcccgtggg gatatccttc
     1621 ttcaccttcc aaacgatgag ctacaccctg gacatcttct accggaaact taaacctgta
     1681 aacagctttg ccgatttcct cttcttcgtg tccttcttcc cccagttggt ggcggggccg
     1741 attctccgcg cttcctggtt cattccccag attaacaaac aactctccct gaacaaggaa
     1801 caggttgcca aggccctgct gctcatcttc accggactgg tgaaaaaagg tgtcattgcc
     1861 gattatatca gcattaactt cgttgaccgc gttttcgaca accctgccct ttattccgga
     1921 ctggagaacc ttctggccct ctatggctat tccctgcagg tctattgcga tttcagcgga
     1981 tattcggata tcgccatcgg cctggccaca atgatgggtt tcgagcttca gccaaacttc
     2041 aattcaccct accgcgcctc ctccctgggc gatttttggc gccgctggca tatatctctc
     2101 agcacctggc tgcgcgatta cctctatatc ccccttggag gcagccgcaa agggaaagtg
     2161 cgaacctatg tgaacctcat gatcaccatg gtgctgggcg gcctctggca cggcgcttcc
     2221 tggaatttcg tgctttgggg cgcgctcaac ggagttggcc tctgcatcga cagattcgtg
     2281 cacaacatct tctggccccg ctccaaagac aaaagcgccc cgccgcgcca aaccccgctt
     2341 ctgctcaaga tcctgggcat tctcttcact ttcaacttca taaccttcac ccggattttc
     2401 ttccgcagcc gcacttttga cgacgccctc accatgatgg agcgcatttt taccaacctc
     2461 aatccgcgct tgttgttcat ctgg
//
LOCUS       DAOOWR010000252         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_42272_length_2476_cov_3.316398, whole genome shotgun sequence.
ACCESSION   DAOOWR010000252 DAOOWR010000000
VERSION     DAOOWR010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42272_length_2476_cov_3.316398"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLB85_06165"
     CDS             complement(<1..1173)
                     /locus_tag="PLB85_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72950.1"
                     /translation="MNKSFWRPFLITAGAIVLVAVLAPLVPARESKVFSIKKLDPIKT
                     LMAPPEVDSAADSLSEKAKQIHIASELQQLEPFLQKLQQQGQNRSGNLRIAFFGDSII
                     EGDLLTGKLRELLQAQYGGHGVGLVPITSIVHGFRQTIRHEFSRNWETVSFMKRGSDK
                     YPLGMVGYTFIPRTWGYEESVIETEAIPEVLDSLGNVISTGQEASSRKETRRFNVSGP
                     AWVEYSGVKTTGGASEFRRIRLFYSRASANSTVRVSLNGGEQTTYALTPGEGVQMLDL
                     SPATPVTRIRLTFSPTDPIHVFGISFDDLSGVYVDNFAVRGFSGMYFNAIPAEYMGAF
                     QRYLNYDLVVLQYGGNVSSPKVRDYSRYKKGMTESIRHIQSAMPGVPILVIGVQDRS"
     gene            complement(1170..>2476)
                     /locus_tag="PLB85_06170"
     CDS             complement(1170..>2476)
                     /locus_tag="PLB85_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72951.1"
                     /translation="ADMDGNCYFTGFCTGPVVFNGQTYPHSGMWDVFYGKLNPAGDLL
                     WFKTFGGSLNDIGYGIETSRDGSIYLTGWFADTIVFSDDISISSYGGSDIFLLRCDTD
                     GNPLWARHAGGPGVEYGYKVDTFSTNTWSVAYITGSASPGSIFGDIVTDANGMFVACY
                     NSDGSTAWVLPSYGVGAINIAADEHLTPTINAVIGRVTGTGSIGGTTLNSVNGSDDIY
                     IAHFDAAGNWLDVEHYGGTGSDKGRAVDIVGVNKEISLLSFEEEISFGNWTLVSNGAW
                     DIALKDEFHPPVSAGSMYSDVGSDVKWLGDDKFVVSGWFSGVMRFGNHVLDSGSETNQ
                     DCFVAIYDYTATDVNDPALVPSPSLNCHPNPGSSSFQIKAQNATEARIYNSRGQLVRN
                     LRSVSDGAFCWDANNETGKRCPSGVYLIKAGNQTGKVLLVDE"
BASE COUNT          561 a          633 c          722 g          560 t
ORIGIN      
        1 gctgcggtcc tgaactccga tcactaggat gggaacgcct ggcatggcac tctggatgtg
       61 gcggatggat tccgtcattc cctttttgta gcgtgaataa tcccggacct tggggctgga
      121 aacgtttccg ccgtattgaa ggaccaccag atcgtaattc aggtaacgct ggaaagcgcc
      181 catgtattcg gcggggatgg cgttgaaata cattccggaa aaaccgcgca cggcaaaatt
      241 gtccacatag acgccgctaa ggtcatcgaa ggaaatgccg aatacgtgaa tgggatctgt
      301 ggggctgaaa gttagcctga tcctggtaac gggagtggct ggacttaaat ccagcatttg
      361 aacaccttcg cctggggtga gggcgtaggt tgtctgctcg cctccgttta gggaaacccg
      421 aacggtggaa ttggccgagg cccgggaata gaaaaggcgg atacggcgga attctgaagc
      481 tccgccagtg gttttgacac cggaatattc cacccaggcc gggccgctaa cgttgaagcg
      541 gcgggtttct ttgcgcgaac tggcttcctg gccggtagag atcacattgc caagggaatc
      601 aagcacttcc gggatggctt cggtctcgat aacggattct tcgtagcccc aggtgcgggg
      661 aatgaaagtg tagcccacca tgccgagggg atatttgtca gacccgcgtt tcatgaagga
      721 aacggtttcc cagttgcggg aaaattcgtg cctgatggtc tggcgaaagc cgtgaacgat
      781 ggatgtgatg ggcaccaggc cgaccccgtg gcctccatat tgggcctgga gcagttcacg
      841 cagctttcct gtgaggaggt ctccctcgat gatggaatcg ccaaaaaagg cgatgcgcag
      901 gtttccggat cggttttggc cctgctgctg gagcttttgg aggaagggct caagctgctg
      961 caactcgctg gcgatgtgga tctgtttcgc tttctcagag agggaatcag cggcagaatc
     1021 aacctcgggt ggggccatca gggttttaat gggatcgagc ttcttgatgg aaaacacctt
     1081 gctttcgcgg gcgggaacca gcggggctag aactgccacg agcacaatgg ccccggctgt
     1141 tatcagaaaa ggacgccaga aagatttgtt cattcatcca ccaacagaac ttttcctgtt
     1201 tggttgccgg ctttgatcag atagacgccg gaaggacagc gcttgccagt ttcattgttg
     1261 gcatcccagc agaaagctcc atcagacacg cttctcagat tgcggaccag ttgcccgcgg
     1321 gagttgtaaa ttctagcttc ggttgcgttt tgggccttga tttgaaagct gctgctgcct
     1381 ggattgggat ggcagttaag acttggcgag ggcaccaggg ccggatcgtt tacgtctgta
     1441 gcagtgtagt cgtaaattgc cacaaagcag tcttggttgg tttccgaacc gctgtccaga
     1501 acatgattgc caaagcgcat cacgccactg aaccagccag agaccacgaa tttatcgtcg
     1561 cccagccatt tgacatcgga tcccacatcc gaatacatgc tgccggcgct caccggagga
     1621 tgaaattcat ccttcagcgc tatgtcccag gcgccattgg aaaccaatgt ccaatttccg
     1681 aaagaaatct cttcctcaaa ggagaggagg gatatttctt tgttcacgcc aacgatatcc
     1741 accgcgcgac ccttgtcgga gcctgtgccg ccataatgct caacatccag ccagttaccg
     1801 gctgcgtcga aatgggcgat ataaatgtca tcggaaccgt tcacgctgtt gagggtagtc
     1861 ccgccgatgc tgccagtgcc ggtcactctg ccgataacgg cattgatggt gggggtgagg
     1921 tgctcatcag cggcgatgtt tatcgcgcca accccgtagg agggtagaac ccaggcagtg
     1981 gagccatcgc tgttgtagca ggccacgaac atgccgttgg cgtcagtgac aatatccccg
     2041 aaaatactac ccgggctggc gctgcctgtg atgtaggcaa cggaccaggt gttggtggaa
     2101 aaagtatcca ctttgtagcc atattctact cccgggccgc cggcatgacg agcccaaagg
     2161 ggatttccgt ctgtatcgca gcgcagtaaa aagatgtcgc tgccgccata actgctgatg
     2221 cttatgtcgt ctgaaaacac aatggtgtcc gcgaaccagc ctgtgaggta gatgcttccg
     2281 tcccttgagg tttcgatgcc gtagccgatg tcattgagtg agccgccgaa ggtcttaaac
     2341 cagagcaaat cacccgctgg attgagtttg ccgtagaaaa catcccacat tccggagtgg
     2401 ggataggttt ggccgttgaa aaccaccgga cccgtgcaaa agcctgtgaa atagcagttg
     2461 ccgtccatgt ctgccg
//
LOCUS       DAOOWR010000253         2457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_42733_length_2457_cov_3.830142, whole genome shotgun sequence.
ACCESSION   DAOOWR010000253 DAOOWR010000000
VERSION     DAOOWR010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42733_length_2457_cov_3.830142"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLB85_06175"
     CDS             <1..684
                     /locus_tag="PLB85_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOU72952.1"
                     /translation="PWLLDTMLGFAAGVMIAASFWSLLNPAINLSNGNPLPALVGFLL
                     GGAFLRGVDALLPHLHPGKEMGEQEGLKTPWGKSTLMFLAVTLHNFPEGLAVGVAFGA
                     YASGFADATLAGAMALTLGIGIQNLPEGAALSIPLRREGMSRWKSFFWGQFSGMVEPL
                     GGLLGAALVMVARPVLPYALAFAAGAMIFVVAEEVIPESQKGEHSHLATLGVMLGFAL
                     MMFLDVALS"
     gene            complement(689..1240)
                     /locus_tag="PLB85_06180"
     CDS             complement(689..1240)
                     /locus_tag="PLB85_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOU72953.1"
                     /translation="MSATEKLLLHSCCAPCLIAPYQELKAEAVNVSVLWYNPNIHPVT
                     EYRQRLQTLKDFAEREGFPLIVKDDYGLRDFVKAVVDRLNSRCGHCYRLRLETAAKTA
                     SENGFDAFSSTLFYSKYQNHELLKEIAEEMAAKYQVSFFYRDWRELWDEGTRLSRESG
                     MYRQKYCGCVFSEEERYLKRKQA"
     gene            complement(1227..1562)
                     /locus_tag="PLB85_06185"
     CDS             complement(1227..1562)
                     /locus_tag="PLB85_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU72954.1"
                     /translation="MHEWIRDFPAAITVCDRDGLILEMNEKACATFASRGGAALIGTS
                     LFDCHKPGSVAIIRDMLATGKNNVYTIEKQGVKKLICQQPWYQNGELGGLVEISLELP
                     NTMAHHVRD"
     gene            complement(1562..>2457)
                     /locus_tag="PLB85_06190"
     CDS             complement(1562..>2457)
                     /locus_tag="PLB85_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU72955.1"
                     /translation="LAFGVTGLGMYPIAIAGSEEQKKKYLPQIAAGEQLAAFALTEAN
                     AGSDAGAIETTARKEGDHYILNGTKQWITNGGEAGIYTVFAMTDKTKGARGCSCFIVE
                     KGTPGFNFGKKENKMGIRASATRELIFEDCAVPEANLLGREGTGFITAMKVFDKSRPM
                     VGSQAVGIARGAFEAAVKYSKERHQFGKPISSFQAVQFMLADMATQIEAARALVWQTA
                     KMIDAGERNFSKESAMCKYFASDMAMQVTTDAVQILGGYGYMKEYPVEKMMRDAKILQ
                     IYEGTNQIQRGIVASNLLKEF"
BASE COUNT          455 a          699 c          705 g          598 t
ORIGIN      
        1 ccctggctgc tggacaccat gctgggtttt gccgcgggag tgatgattgc ggccagcttc
       61 tggtctctac tcaatccggc catcaacctc agcaacggaa atcctctgcc agccttggtc
      121 gggtttttgc tgggaggggc attcctgcgt ggagtggacg ctctgttgcc acatctacac
      181 ccgggcaaag agatgggcga acaagaaggc ctgaaaacac cctggggcaa atcaaccctg
      241 atgttcctgg ctgtcacgct ccacaatttc ccggaaggac tggctgtggg cgtggctttc
      301 ggagcttacg cttccggatt tgccgatgcc actctggccg gggctatggc cctcactctg
      361 ggcatcggga tccagaacct gcccgaaggc gcggccctct ccattccgct aaggcgggag
      421 gggatgtcga ggtggaaaag cttcttctgg ggccagttct ctggcatggt ggaaccgctg
      481 gggggcttgc tgggagcggc tttggtgatg gttgcccgtc cggttttgcc atacgcgctg
      541 gcctttgccg ccggagccat gatcttcgtg gtggcggaag aggtgatccc cgaatcgcaa
      601 aaaggcgaac actcgcacct ggccactttg ggcgtgatgc tcgggttcgc cctgatgatg
      661 tttctggacg tggccctgag ctgaaggctc aggcctgttt ccgctttaga tagcgctctt
      721 cctcgctgaa cacacagccg cagtatttct ggcggtacat tccggattcc ctggaaagcc
      781 gtgttccctc atcccaaagc tctctccagt cgcggtaaaa gaagctcact tggtactttg
      841 ccgccatctc ctccgcaatc tctttcagca gttcgtggtt ttggtatttg ctatagaaca
      901 gcgtgctgga aaaagcgtca aaaccgtttt cggaagccgt tttggctgct gtttccaacc
      961 tcagtcggta gcagtgcccg catctggagt tgagcctgtc cacaaccgct ttcacgaagt
     1021 ccctgaggcc gtaatcatct ttaacgatca gcggaaagcc ctcccgctcc gcgaaatcct
     1081 tcagcgtttg caaccgctgg cggtattccg taacgggatg gatgttgggg ttgtaccaca
     1141 gcaccgaaac gtttacagct tcggctttca actcctggta cggggcgata aggcaaggcg
     1201 cgcagcagct gtgcaggagc aacttttcag tcgcggacat ggtgggccat ggtattcggc
     1261 agttccagcg atatttccac caaaccgccc agttctccat tttgatacca gggctgctgg
     1321 cagataagct ttttcacacc ctgcttttca atggtgtaaa cgttgttctt tcctgtagcc
     1381 agcatatccc tgatgatggc tacggagccg ggtttgtggc aatcgaagag gctggtgccg
     1441 attagcgccg cgccaccgcg ggaagcgaaa gtggcgcagg ctttctcgtt catttccagg
     1501 atcaggccgt ccctgtcgca gaccgtgatg gcggctggaa agtccctgat ccattcgtgc
     1561 atcaaaactc cttaagcagg ttcgaggcca cgatccctcg ctggatctgg tttgtgcctt
     1621 cgtagatctg caggatcttg gcgtcgcgca tcatcttttc cactggatat tccttcatgt
     1681 agccataacc gccaaggatt tgcacggcgt cggtggtaac ctgcatcgcc atgtccgaag
     1741 cgaaatattt gcacatggcg gattccttgc tgaagttgcg ctcgccggca tcgatcatct
     1801 tcgccgtctg ccaaaccaaa gcgcgggctg cctcaatctg agtggccatg tctgccagca
     1861 tgaactggac agcctggaag ctcgagatag gcttgccgaa ctgatgcctt tccttggaat
     1921 acttcaccgc cgcttcaaaa gcgccgcggg cgatgcccac agcctgagaa cccaccatgg
     1981 ggcgggattt gtcgaaaacc ttcatcgcgg tgatgaagcc agttccttcg cggcccagca
     2041 gattggcctc gggtacggcg cagtcctcga agatcaattc ccgtgtggct gaagctcgga
     2101 tgcccatctt gttttccttc ttgccaaagt tgaagccggg agtgcctttt tccactatga
     2161 agcaggagca gccccgggcg cccttggttt tgtccgtcat ggcgaaaact gtgtagatgc
     2221 ctgcctcgcc gccgtttgtg atccattgtt tggtgccgtt gaggatgtag tggtctcctt
     2281 ctttgcgggc agttgtttcg atcgctccgg cgtcagaacc ggcgttcgct tcggtgagcg
     2341 cgaaagcggc aagctgctcc ccggcagcga tctggggcag atatttcttt ttctgctctt
     2401 ccgagccggc gatagcgatg ggatacatgc ccaggccggt gacgccaaag gccagcg
//
LOCUS       DAOOWR010000254         2453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_42830_length_2453_cov_3.633445, whole genome shotgun sequence.
ACCESSION   DAOOWR010000254 DAOOWR010000000
VERSION     DAOOWR010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42830_length_2453_cov_3.633445"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..561
                     /locus_tag="PLB85_06195"
     CDS             97..561
                     /locus_tag="PLB85_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOU72956.1"
                     /translation="MGRNRILRQVLATPAVKGFRPFGRIGGKRPKIIMLLDEYEAIRL
                     LDHENLTQEEAAEAMNVSRPTLTRIYDRARRKFASALVEGALLLIEGGDIRLKQHDWL
                     CEDCGAFTESATPHVFHCPVCTSPHLISLGDCYQNQCHKCKKCHRGGRNARI"
     gene            548..772
                     /locus_tag="PLB85_06200"
     CDS             548..772
                     /locus_tag="PLB85_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOU72957.1"
                     /translation="MPGFDGTGPRGSGRPGRGLGCRNRFGQGQAGKNSLPKQGYVYEY
                     TLEELKERKQALEEEIRWIDDRIGELEKES"
     gene            860..1186
                     /locus_tag="PLB85_06205"
     CDS             860..1186
                     /locus_tag="PLB85_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU72958.1"
                     /translation="MKIAIPLTNGELSSHFGHCEKFAIYTIDNKEILKEEIVDPPAHE
                     PGSHPAFLHKLGCNVVIAGGMGFKAQELMSQNGITVVMGVPNLPLKGTGGSISARESG
                     IRPKQV"
     gene            1193..2056
                     /locus_tag="PLB85_06210"
     CDS             1193..2056
                     /locus_tag="PLB85_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU72959.1"
                     /translation="MKIAIASGKGGTGKTTLAVNLALRASETRNVVLADLDVEEPNSS
                     IFISGNKLIEQQLFRSVPNWEKEACTLCGKCAEVCQFHAVVKLGETIMVLPKLCHSCF
                     ACSELCPVNALPMRNTPLGWMRVFEQGKLFFLESKLDVGEEMSAPMIKQTIAYMNDKL
                     PEPEYQFRDCPPGTSCPVIAATGDADLVLLVTEPTPFGLHDLELAVEAMRQLDKRMAV
                     VINRWGIGNDKVLRFCETERLPVWAIFPNQRKIAELYSRGELIYPKVQEFREGLDSIL
                     DKLDLMKREAE"
     gene            2053..>2453
                     /locus_tag="PLB85_06215"
     CDS             2053..>2453
                     /locus_tag="PLB85_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU72960.1"
                     /translation="MKEIVIISGKGGTGKTSVAAALATIASQTVMADCDVDAADLHLI
                     LKPETRKSEAFFSGVKAVIDPNKCSSCGLCQDICRFDAVCHQGSVYRIDPLDCEGCGY
                     CHHICPVDAIDLQEQQVGYCYISDTKYDCQLV"
BASE COUNT          645 a          613 c          683 g          512 t
ORIGIN      
        1 tttcattgcc agggtgttgc atggatccgc agaaatgtgt tgacagaaaa gccggtgctc
       61 cagattttga acatatgttc attaaacgag aagacaatgg gtagaaacag aattcttaga
      121 caggttttag ccacgccggc ggtgaaaggt tttcggccct ttggccggat cgggggaaag
      181 cgcccgaaga taatcatgct gctggatgag tatgaagcga tccggttgct ggaccacgaa
      241 aacctcaccc aggaggaggc cgcggaggcc atgaacgttt cgaggcccac gcttacccgc
      301 atctacgacc gggcgcggcg gaaattcgcc tcggcgctgg tggagggcgc cctcttgctg
      361 atcgaaggcg gcgacatccg gctgaaacaa catgactggc tttgcgagga ttgcggagcc
      421 ttcacggaaa gcgccacgcc gcatgttttt cactgcccag tctgcacatc gcctcatctc
      481 atatctctcg gcgattgtta ccaaaatcaa tgccataagt gcaaaaaatg ccaccgagga
      541 ggtagaaatg cccggatttg acggaacagg acccaggggc tcaggccggc ccggacgcgg
      601 tttgggatgc cgcaacagat ttggccaggg acaagccggc aaaaactctc ttccaaaaca
      661 aggatatgtt tacgaatata ccctggaaga gcttaaagaa cgcaagcagg ctttggaaga
      721 agaaatccgc tggatcgatg acaggatcgg cgaactggaa aaagaaagct gaaaacgccc
      781 accaaatatg tgatggtaaa atcacggaag ccgctatcag caaaatgaaa aactgaaaaa
      841 acaataaagg aagcaagaaa tgaaaatagc cataccatta accaatggag aactgagctc
      901 gcatttcgga cattgcgaga aattcgccat ctacaccatc gacaacaaag agatcctgaa
      961 agaggaaatt gtagatccac cagcgcacga accgggctcg catccggctt ttctgcacaa
     1021 acttggctgc aatgtggtga ttgccggggg catgggcttc aaggcccagg aactgatgag
     1081 ccaaaacggc atcaccgtgg tgatgggcgt tccaaacctg ccgctgaaag gaactggtgg
     1141 ctcgatatct gcaagggaat ctggaatccg gccaaaacag gtgtgaccat tgatgaaaat
     1201 cgccattgcc agcggcaagg gtggaacagg gaaaaccacc ctggcggtga atctggctct
     1261 gcgggcatct gaaacacgaa atgtggtgct ggccgatctg gatgtggagg agccaaacag
     1321 ttccatcttc atcagtggaa acaagcttat agaacaacag cttttccgtt cggttcccaa
     1381 ctgggaaaaa gaagcctgca cgctttgtgg caaatgcgct gaggtgtgcc agttccacgc
     1441 tgtggtcaaa ttgggggaaa ccatcatggt gttgcccaag ctttgccatt catgctttgc
     1501 ctgcagtgaa ctctgtcccg tgaacgccct tcccatgcgg aatacaccgc tgggctggat
     1561 gcgcgtattt gagcagggca agcttttttt cctggaaagc aagcttgacg tcggagaaga
     1621 aatgtctgcg cccatgatca aacagaccat cgcctacatg aacgacaagc tgccggagcc
     1681 agaatatcag tttcgcgatt gccctcccgg aacgtcatgc ccggttatcg ccgccaccgg
     1741 cgacgcggac cttgttctgc tggtgaccga gcccacaccc tttggccttc acgaccttga
     1801 gctggctgtg gaggcgatgc gccaactgga taaaagaatg gctgtggtga tcaaccgctg
     1861 ggggatcggc aacgataaag tgttacgctt ttgtgagact gaacggcttc ctgtctgggc
     1921 tatcttcccc aatcaacgca agatagcgga attgtattcc cgaggtgagc tcatctatcc
     1981 caaagtgcag gaattccggg aaggcttgga ctccattctg gataaactgg atttgatgaa
     2041 aagggaggcc gaatgaaaga gatagtcatc atctccggca aaggcggcac aggaaaaacc
     2101 tctgtcgctg cggctttggc cacgatcgct tcccagacag tgatggcgga ttgtgacgtt
     2161 gacgcggctg atctgcatct gatcttgaaa ccggaaacgc ggaagagcga agcttttttc
     2221 agcggggtca aggccgtgat cgatcccaac aaatgcagct cctgcgggct ctgccaggat
     2281 atttgccgct ttgacgccgt gtgccatcag ggatcagttt acaggatcga cccgctggat
     2341 tgtgaaggtt gtggttactg ccatcacatc tgccctgtgg acgcgatcga cctgcaggaa
     2401 caacaggttg gctactgcta catctccgat accaaatatg attgccagtt ggt
//
LOCUS       DAOOWR010000255         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_43212_length_2435_cov_3.973950, whole genome shotgun sequence.
ACCESSION   DAOOWR010000255 DAOOWR010000000
VERSION     DAOOWR010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43212_length_2435_cov_3.973950"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1296)
                     /gene="clpX"
                     /locus_tag="PLB85_06220"
     CDS             complement(52..1296)
                     /gene="clpX"
                     /locus_tag="PLB85_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU72961.1"
                     /translation="MDKFKNLSCSFCGRNELEVKTLIRGIAGNICDECIAMCHNIVVT
                     TAPETSEEVVELPRPSEIKSYLDQYVIGQEQAKMIISVAVYNHYKRIFRQDDKDSIEL
                     EKSNIMMIGPTGCGKTLIAQTLANYLKVPFAISDATTLTEAGYVGEDVENILVRLLQA
                     ADYDVAKAERGIVYLDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIVGGAFFGLEKIIKERLDTKAIGFDVKLGRVTEEYNIFD
                     HTVSSDLLKYGLIPELIGRMPVLCTLHELSEEALVDILTQPKNAICSQYKKYFQMDGV
                     DLEFDHEALREIASIAIKQKAGARGLRAIMEKFMLKIMYDIPDMEKLRGCRISADVVS
                     KGSAPAFTFAGSTARRKAVGSS"
     gene            complement(1372..1956)
                     /gene="clpP"
                     /locus_tag="PLB85_06225"
     CDS             complement(1372..1956)
                     /gene="clpP"
                     /locus_tag="PLB85_06225"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOU72962.1"
                     /translation="MNYIPMVIEQVGRTERAYDIYSRLLKDRIVFVSGVIEDNLANSI
                     VAQLLHLEGEDPERDIYMYINSPGGIISSGLAIYDTMQYIKPNVSTICIGQAASMAAL
                     LLAAGHHGKRTALPNSRIMIHQPLGGAQGQASDIEIQAREILYLRERMNEILHKHTGQ
                     DLKKLVEDTDRNFYMDALQAKEYGIIDEMIVTRK"
     gene            complement(1953..>2435)
                     /locus_tag="PLB85_06230"
     CDS             complement(1953..>2435)
                     /locus_tag="PLB85_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72963.1"
                     /translation="EHVNYDGENSAILAKLYNDNQFPLPKKTLQYLVVQELESFDERY
                     RQMLQKYVIDKVVQDMVSMYLLAALQKQSGTEFSEEMKDAYIEHRAILDEKNPAAYRE
                     QNQEMIDGENFRESALNYHILRGIATTCIFVEPEPEPEPEQPEDTEFEVSKETEEEQE
                     "
BASE COUNT          453 a          648 c          656 g          678 t
ORIGIN      
        1 aaccatgccc agcagcaaca gcagcagccc gtgtttcatt gcataggccc atcaactcga
       61 accgaccgct tttctgcggg cggtgctgcc ggcaaaggtg aatgccggtg cgctgccttt
      121 gctcaccaca tccgcgctga tgcggcaacc gcgaagtttt tccatatccg gaatgtcata
      181 catgattttc agcatgaact tttccatgat ggcacgcagc ccgcgggcgc ccgctttttg
      241 cttgatggcg atgctggcga tctcgcgcag ggcttcgtga tcgaattcca ggtccacacc
      301 atccatctgg aagtatttct tgtactggct gcagatggcg ttcttgggct gggtgaggat
      361 atccaccagc gcttcttcgc tgagttcgtg cagagtgcag agcaccggca tgcggcctat
      421 cagttctggg ataaggccgt atttcagcag atcgctggac acagtatggt caaaaatgtt
      481 gtattcttcg gtcactcggc ccagcttcac atcgaagccg atggccttgg tgtccaggcg
      541 ttccttgatt atcttttcca ggccgaagaa agccccgcca acgatgaaga ggatgttttt
      601 ggtatccatt tcgatgaagt cctgctgcgg gtgtttgcgc ccgcctttgg gtggaacgtt
      661 caccttggtg ccttccagga ttttcagcaa agcctgctgc acgccttctc cggagacgtc
      721 gcgggtgatc gagggggttt ctgatttgcg gctgatcttg tcgatttcgt ctaaatagac
      781 gatgccgcgc tctgccttgg ccacatcata atccgcggct tgcagcaaac gcaccaggat
      841 gttttccacg tcttcaccaa cgtagccagc ttcagtgaga gtggtggcgt cggaaatggc
      901 gaagggcacc ttcagatagt tggccaaagt ctgcgcaatc agtgtcttgc cgcagccagt
      961 aggtccgatc atcatgatat tgcttttctc cagctcgatg gagtctttgt cgtcctggcg
     1021 gaagatgcgt ttgtaatggt tatagaccgc tacggagatg atcattttag cctgttcctg
     1081 gccgatcaca tactggtcca gataactctt gatctcgctg ggccggggca gttcgaccac
     1141 ttcttctgag gtttcaggag cggtggtcac cacgatgttg tggcacatgg cgatgcattc
     1201 atcgcagata ttccctgcaa tcccgcggat cagggttttc acctcgagct cgtttctgcc
     1261 gcagaaagag cagctcaggt tcttgaactt atccaagaaa tcctcctggt tttctttgtt
     1321 cggtttggat gccttagttt cactaattgt accgttgtga atcccgtcaa gttatttccg
     1381 ggtgacgatc atttcgtcga tgatgccata ttctttggct tgcagggcgt ccatgtaaaa
     1441 gttgcggtcg gtgtcctcca ccagtttttt caaatcctgt ccggtgtgtt tgtgcaggat
     1501 ttcgttcatc ctttcgcgca ggtagaggat ttcacgggcc tggatttcga tgtcgctggc
     1561 ctggccttgc gcgccgccga ggggctggtg gatcatgatg cggctgttgg gcagggcggt
     1621 gcgtttgccg tgatgcccgg cagccagcag cagcgctgcc atgctggcgg cctggccgat
     1681 gcagatggtg ctcacgttcg gcttgatgta ctgcatggtg tcgtatatcg ccaatccgga
     1741 ggagattatc cctccggggc tgttgatgta catatagatg tcgcgttcgg ggtcctcgcc
     1801 ttccagatgc aaaagctgcg ccactatgga attggccagg ttgtcctcga tcacaccgct
     1861 cacaaagact atcctgtctt tcagcaggcg cgaatagatg tcgtaagccc gctcggtgcg
     1921 acccacctgt tctatcacca tgggaatgta gttcattcct gttcctcctc ggtttcctta
     1981 gaaacctcaa actcggtgtc ctccggctgt tccggctccg gttccggttc aggttccacg
     2041 aaaatacaag tggtagcgat cccacgcagg atatgatagt taagcgcgct ttccctgaaa
     2101 ttctctccgt cgatcatttc ttggttctgc tcgcggtacg ctgcgggatt cttttcatcc
     2161 agtatggccc tgtgttcgat ataggcgtct ttcatctctt cagaaaattc cgtaccgctc
     2221 tgcttttgca gcgcggccag cagatacatg ctcaccatgt cctgcactac cttgtctatc
     2281 acgtatttct gcagcatttg gcggtatctc tcgtcgaaac tttccagttc ctggaccacc
     2341 aggtattgca aggtcttttt gggcagggga aactggttgt cgttataaag cttggcgagg
     2401 atagcggagt tttcgccatc ataattgacg tgctc
//
LOCUS       DAOOWR010000256         2386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_44479_length_2386_cov_2.809524, whole genome shotgun sequence.
ACCESSION   DAOOWR010000256 DAOOWR010000000
VERSION     DAOOWR010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44479_length_2386_cov_2.809524"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            689..1063
                     /locus_tag="PLB85_06235"
     CDS             689..1063
                     /locus_tag="PLB85_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU72964.1"
                     /translation="MKIFNGDTPIYLQLRAHIEELILEGTLAEEAQIHSQRVMASEYG
                     LNPITVGNALSLLVDEGVLEKKRGVGMFVALGAREKIINALSKDFITDSLEPVLRKAR
                     QLELPKALITQSLNKIYGGKDE"
     gene            1056..1913
                     /locus_tag="PLB85_06240"
     CDS             1056..1913
                     /locus_tag="PLB85_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU72965.1"
                     /translation="MSEFIYQTSNLTKFYGKFQALKDVNLSLGRGRIIGLLGKNGAGK
                     STLMRCLLGFLNHKGSVKLGDMEIKHRDPRVFEEAAFIPDVSGLDDRLTVRQTIEYVR
                     GVNPRWNDSRAQKLLSVSNLPPKFKVGKLSKGMKTKLYLLITLSLDVSFLMLDEPTLG
                     LDIAFRKEFFDTILGEFFDESKTILISTHQAEEVEHLLQEVIFIDHGQILLHEEMESL
                     KSRLRVVTLPSNREAEIMAHKPRLISRTLGAVSAVLDAEVEISGASYGRANLTDLFLS
                     IVGGTDETV"
     gene            1900..>2386
                     /locus_tag="PLB85_06245"
     CDS             1900..>2386
                     /locus_tag="PLB85_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72966.1"
                     /translation="MKQCKALLKKEWDTNWFMALIPLWATCIVYITALIGWIISLIRG
                     HSLGAMVSSGEILTGYSDLVLYGLTSAITIMLGFVSAISAVVLAYNVINGGYKNRCEI
                     FHFSQPVSFLKIAGSKFLLVSLVTILLYGLLSLLNAVVFTLIYSNSVSAGFRYGLTGW
                     MQ"
BASE COUNT          591 a          600 c          648 g          547 t
ORIGIN      
        1 cggcctttct gtaaaggctc ctgttcttac tgcaggccag ggtaagcagc attagcgcga
       61 gcaggctggc tgtcagtatt cgtttgagca gcgtcaatat tcctccagat cgtttatgaa
      121 aataggttat ctttaccaaa agccacgcct tggccgcctt gtcaagaaaa agttgcgccg
      181 ctccaagagg tctatttttt agattgtatg gcctcgcccc aacgcagatt tccgctctgc
      241 cagcacagac ggcatccggt tcttttactg cttcccggaa caagtccgcg atggcgaaag
      301 tggagaaatc ccgggggcaa gaagggtgaa tcggtgaatt tagagaatca ttaaaaaagg
      361 ccacccgggg tacagggtgg tcatcccggt cccgacatca gcttatgtca acatctatca
      421 ggacagggtt aagagtgtat ttgagccttc ctttttgagg tatcttactt ctccatagca
      481 tatcagctta tcccatcgga aaaatgggat aaagtgaaag agtggcgttg cgcacctacc
      541 taataccgaa gatatcgtat tttataacta cctgcttcag aacgtcttat aacaatgcca
      601 gaaaaagttc ttgacaagaa tgtggtagtg ttatggttgg ctacatcact atggtgatac
      661 tgcagtacat aaggttaaag aggtggttat gaagatattc aacggcgaca cgccaatcta
      721 cctgcaactt cgagcccaca tcgaggagct gatcctggag gggactctgg ccgaagaggc
      781 acagatccac tcccaaaggg tgatggctag cgagtatggg ctgaatccca tcacggtggg
      841 caacgccctc agccttctgg tggacgaagg cgtgctggaa aagaagcggg gagtggggat
      901 gtttgtagcc ctgggcgccc gggagaagat catcaacgcc ctcagcaagg atttcatcac
      961 ggacagcctg gagccggttc tgcgcaaagc ccggcaattg gagctgccca aagcgttaat
     1021 aacgcaaagc ttgaacaaga tatatggagg aaaggatgag tgaattcatc taccaaacga
     1081 gcaacctgac gaagttctac ggcaaattcc aggctctgaa agacgtgaac ctgagcctgg
     1141 ggcgcggccg gatcatcggc ttgctgggca agaacggagc gggtaaatcg accctgatgc
     1201 gctgcctttt gggttttctg aaccataagg gcagcgtgaa actgggggat atggaaatca
     1261 agcaccggga cccccgcgtt tttgaagaag cggccttcat cccagatgtg agcggattgg
     1321 acgaccgcct cacggttcgc cagaccatcg aatacgttcg gggcgtgaat ccccgctgga
     1381 acgacagccg cgcccaaaag cttctgtcag tcagcaatct gcccccgaaa ttcaaggtgg
     1441 gcaaactctc caaaggcatg aagaccaaac tctacctgct tatcaccctc tcgctggacg
     1501 tgagctttct gatgctcgac gaacccacgc tgggactgga cattgccttt cgcaaggaat
     1561 ttttcgacac catcctgggc gagttcttcg acgagagcaa gaccatcctc atctccactc
     1621 atcaggcgga ggaggtggag catttgctgc aggaagtaat cttcatcgac cacgggcaaa
     1681 tcctgcttca cgaagagatg gaaagcctga aaagccggct gcgggtagtc actctgccta
     1741 gtaaccggga agctgaaatc atggcgcaca agccccgcct gatctcacgc accctgggcg
     1801 ctgtgagcgc cgttctagac gcggaagtgg agatttccgg agccagttac ggccgggcca
     1861 atctcacgga ccttttcctt tcaattgtgg gaggtacaga tgaaacagtg taaagcgctg
     1921 ctcaaaaaag aatgggacac gaactggttc atggcactga ttccgctgtg ggcaacctgc
     1981 atcgtgtaca tcaccgccct gatcggctgg ataatcagcc tgatccgggg acacagcctg
     2041 ggtgccatgg tgagctcggg agaaatactc accggatata gcgacttagt tctttatggt
     2101 ttgacttcgg ctataacaat catgctgggc tttgtttccg cgatcagcgc tgttgttttg
     2161 gcgtacaacg tgatcaacgg gggctataaa aaccgctgtg agattttcca tttttcccag
     2221 ccggtgtctt tcctgaaaat agccggcagc aaattcctgc tggtgagcct ggtaacgata
     2281 ctgctgtatg gactgctatc gctgcttaac gccgtggtgt tcaccctgat ttacagcaat
     2341 tccgtttcgg cgggctttcg gtatggcctg acaggctgga tgcaga
//
LOCUS       DAOOWR010000257         2377 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_44722_length_2377_cov_3.580965, whole genome shotgun sequence.
ACCESSION   DAOOWR010000257 DAOOWR010000000
VERSION     DAOOWR010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44722_length_2377_cov_3.580965"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLB85_06250"
     CDS             <1..862
                     /locus_tag="PLB85_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU72967.1"
                     /translation="RLLRVCRHFYLDEFPYIRDSGQHTGSGDNWHRLGGLKLFADGSL
                     GSQTGAIFGEYPHSKGNRGILRHSEEEIFALAKQAAEHGFSCLVHAIGDRAVFTVIQA
                     LLRLRQSGIKPPSPFRIEHLQSIRPQDIPLLKECGACCSIQPVHLANDVDMIEDHWRQ
                     IKHEAYSFRSILDAGIPVSFGSDAPIETISPFAGIYSAVERRKNLDPRKPSWLPEQRI
                     SAHEAIYAYTLGAAKASGAEGWTGSLTPGKVADLIVLRDFTALPPDYWLEASALLTML
                     DSRIVFRDNI"
     gene            884..2263
                     /locus_tag="PLB85_06255"
     CDS             884..2263
                     /locus_tag="PLB85_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HOU72968.1"
                     /translation="MAKLGFDFPATPRRTFLYELEENWYLPLLAQKKGLPLQDIKRSN
                     FGEYSMAVNYLTSVLDEAAAINNLAIYNIDHMGQILFTGKDAPALLDRALAGRMSDMK
                     IGACKYTLLLNEQGGVQDDMIFMRLSETEFIAVINAGHDITDNVNGQELIADIDRIMA
                     CKLDGEEVEATDISDQLVKVDIQGPLSYKLIKQIYGAEVLKNRNNPEKNMSFFTFNKF
                     ERNGARYLISRTGYTNRWGWELYLPVANAAEDFKKIVSLALDLGGLLVGLGGRDENRI
                     SAGPFGLPLMGSEYDPEHNPIHAPLFDAAVDMTKPDFMGKAALEKGFAANPRKALILF
                     VSEGIATNRRVFKDGICLGTVTSSINSPNLSLEQRLALGSKRRNVNAEDGNAAIGLAW
                     LYENPFQKDAEGKFISHENELPIRIPVQLFRVDEAGNASGSPMVAYLTLEGVSTATAP
                     KALKNIENL"
BASE COUNT          566 a          676 c          622 g          513 t
ORIGIN      
        1 ccggctgctg agggtctgcc gtcatttcta tctggatgag ttcccttaca ttcgggattc
       61 aggccagcac acaggcagcg gcgataactg gcatcgcttg ggcggtttaa agctttttgc
      121 tgatggttcc ctgggttcac agacaggcgc gattttcgga gaatatcccc attcaaaagg
      181 caatcgcggc atcctgcgcc acagcgagga agagatcttt gccctggcca aacaagccgc
      241 ggaacacgga ttttcctgcc tggtccatgc catcggcgac cgcgctgtgt tcactgtaat
      301 tcaggccctg ctgcgcttgc gtcaaagcgg aataaagccc ccaagccctt tcaggatcga
      361 gcatttgcaa tccatccggc ctcaggatat cccactattg aaggaatgcg gagcctgctg
      421 ctccatccag cccgtgcatc tggccaacga tgtggacatg atagaagacc actggcgcca
      481 aataaaacac gaggcctaca gtttcaggtc aatcctcgac gccggcatac cggtgagttt
      541 cggttctgac gcccccatcg agacgatcag tccattcgcg ggaatctaca gcgctgtgga
      601 acgcagaaag aatctggacc cgcgtaagcc ttcctggttg ccggagcagc gcatcagcgc
      661 acatgaggcc atctatgcct acaccttggg cgcggcgaaa gcatctggag cggaaggctg
      721 gacaggttcc ctcactccgg gcaaggtggc ggacctgatc gtcctgcggg atttcacggc
      781 acttcctccg gattactggc tggaagcgtc ggcgctgctc accatgctgg acagtcgcat
      841 agttttcaga gataacatat aaaatacata caaggagtca aacatggcta aattaggatt
      901 cgatttcccg gcaacgccgc gtaggacctt cctctacgaa ctggaggaaa actggtatct
      961 gcccctgctg gcgcaaaaga agggcctgcc cctccaggat atcaaacgca gcaactttgg
     1021 cgaatacagc atggcggtga actacctcac ctccgtattg gatgaggccg ccgccatcaa
     1081 caatctggct atctacaaca tcgatcatat gggccagatc ctcttcaccg gaaaggacgc
     1141 tcctgcgttg ttggaccgtg ccctggcagg ccgcatgagc gacatgaaga tcggcgcatg
     1201 caaatacacc ctgctgctga acgaacaagg cggagtgcag gatgacatga tcttcatgcg
     1261 cctttcggaa acggaattca ttgccgtgat caacgccggt cacgacatca ctgacaatgt
     1321 gaacggccag gaactaatcg ctgacatcga ccgcatcatg gcctgcaaac tggatgggga
     1381 agaggtagaa gccaccgata tttcagacca gttggtgaaa gtggacatcc agggccctct
     1441 ctcctacaag cttatcaaac agatttacgg cgctgaagtg ctcaaaaacc gcaataaccc
     1501 ggaaaagaac atgagcttct tcaccttcaa taaattcgag cgcaacggtg cccgctacct
     1561 gattagccgc accggctaca ccaatcgttg gggctgggaa ctctatctgc ccgtggccaa
     1621 tgcggcggag gattttaaaa agatcgtgag cctggctttg gatttgggcg gattgctggt
     1681 tggccttggc ggccgtgatg aaaaccgcat ttccgctggt ccctttggtc tgcctctgat
     1741 gggcagcgag tatgaccctg agcacaatcc catccacgct ccgctcttcg acgctgcggt
     1801 agatatgacc aaaccggact ttatgggaaa ggccgctctg gaaaagggtt tcgcggctaa
     1861 tccccgcaaa gccctgatcc tctttgtatc agaaggcatc gccacgaacc gccgggtttt
     1921 caaggacggt atctgcctcg gcacagtgac ttccagcatc aactccccca atctatcact
     1981 ggagcaacgt ttggctctcg gttccaaacg ccggaatgtt aacgcggagg acggaaatgc
     2041 cgccattggc cttgcctggc tctatgagaa tcccttccag aaggatgccg aaggcaaatt
     2101 catctcccat gagaacgagc ttcccatccg catcccggtt cagctcttcc gggtggatga
     2161 agccgggaat gcaagcggtt ctccgatggt ggcttatctg accctggagg gcgtttctac
     2221 cgccaccgcg cccaaagccc tgaaaaatat cgagaacctc taagcaccac aacaacaata
     2281 cgttaaaccg cgcctgaatc cttaacctgg tattcaggcc ggttttttgt atgcaggatt
     2341 tttggctggg caggtattat ctgtagaatc atccaat
//
LOCUS       DAOOWR010000258         2364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_45056_length_2364_cov_3.479861, whole genome shotgun sequence.
ACCESSION   DAOOWR010000258 DAOOWR010000000
VERSION     DAOOWR010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45056_length_2364_cov_3.479861"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="ssb"
                     /locus_tag="PLB85_06260"
     CDS             complement(<1..379)
                     /gene="ssb"
                     /locus_tag="PLB85_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU72969.1"
                     /translation="MADLKLPRLNRVIIAGRITRDLELKYTPKGSPVVNFSVAMDKRF
                     RDESDQWQSVPVYVDVVAWNQTAENICKQANKGTAVLVEGRIDTRSYTDQNNISRKVF
                     EIIADFIQTLEWKPREEVGEPPLP"
     gene            complement(388..678)
                     /gene="rpsF"
                     /locus_tag="PLB85_06265"
     CDS             complement(388..678)
                     /gene="rpsF"
                     /locus_tag="PLB85_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU72970.1"
                     /translation="MVKNYELMVIISPKLSEEEAAALNESILNLAKEQNGEIVKTDPW
                     GKRMLAYPIDKHTEAYYFVNYLSMEAAGVKGIKQQLNINEQVLRHMFIVKEQ"
     gene            complement(662..1270)
                     /gene="pth"
                     /locus_tag="PLB85_06270"
     CDS             complement(662..1270)
                     /gene="pth"
                     /locus_tag="PLB85_06270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU72971.1"
                     /translation="MKLIMGLGNIGKEYELSRHNAGFLCLELWAKRHHKAFKHGCQFD
                     YLRHREACLIKPNTYMNRSGQALAEARRKWKFSEALVVHDDIELPLASLRLRNGGGDG
                     GHNGMGSLLEIMPAEDLKRIRVGIGRDGSNPRDYVLESFSQSELQLLQPALEKACELI
                     DEYIRGDFNSVLNAYSVWKKSCSGEENPGNKCPKEIDNGKEL"
     gene            complement(1267..1875)
                     /locus_tag="PLB85_06275"
     CDS             complement(1267..1875)
                     /locus_tag="PLB85_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOU72972.1"
                     /translation="MIFTIEAQPKQTSKKSEINALRRQGMIPAVLYGKTVESTPIAID
                     RAKFLQYYKQSFSELTFYEIVLNGNKYHTILKDKLVHPVTRNILHIDFMVVEESAEME
                     FEIPIQIVGEAIGTKEGGFLDVIQRTVKISCKAKDVPEEISLDVSDLKVGDTLHIRNL
                     PTGNWQYKDQDDITLVVVHGKKAEEAPDEGEEPAAEEPKPEQ"
     gene            complement(1897..>2364)
                     /gene="prs"
                     /locus_tag="PLB85_06280"
     CDS             complement(1897..>2364)
                     /gene="prs"
                     /locus_tag="PLB85_06280"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU72973.1"
                     /translation="DIENPVVVSPDSGGANRARALAKRLNCGLAIGDKRRTGNDDHAI
                     LLNIIGDVAGKTAILFDDIIDTGGSLVKIAEVLNNYDVKKIYAACTHGVLSGKVVQSI
                     ENSPLEKLFVTNTIPLSAEAASCPKIVQLSIAEMLAIAIKKVHIEESLSILFK"
BASE COUNT          489 a          590 c          591 g          694 t
ORIGIN      
        1 caggcagggg cggttctcca acttcttccc tgggtttcca ttcgagggtc tggatgaaat
       61 cagcaatgat ttcgaaaacc ttgcggctaa tgttattttg gtcggtataa gatctggtat
      121 cgatcctgcc ctcaaccagt actgcggtgc ctttgttggc ctgtttgcag atgttctcag
      181 ctgtttggtt ccaagccact acatccacat aaaccggaac tgattgccat tgatcggatt
      241 cgtctctgaa acgcttgtcc atggccaccg agaaattcac aaccgggctt cccttggggg
      301 tgtactttag ctcaaggtcc ctggtgatgc ggccggcaat gatcactctg ttcaaccggg
      361 gtaactttag gtctgccatt tttcctccta ttgttccttc acgatgaaca tgtgccggag
      421 cacctgctca ttgatgttca gctgttgttt gatgcctttc acaccagcag cttccatgct
      481 gagataattc acgaagtaat aagcctcagt gtgtttgtcg atgggataag ccagcattcg
      541 cttgccccag ggatcggttt tcacgatctc tccgttttgt tctttggcga ggttgaggat
      601 gctttcgttc agggcagcag cttcctcttc gctgagctta ggtgagatga tcaccataag
      661 ttcatagttc tttaccattg tctatctcct ttggacattt gtttccggga ttttcctccc
      721 cggaacagga ttttttccaa acgctatagg cgttcaacac cgagttgaaa tcgccgcgta
      781 tatattcgtc tataagttca caagcctttt ccagcgcagg ttgcagcagt tgcaactctg
      841 attgggaaaa gctttccaaa acgtaatccc tggggttgga gccgtcgcgg ccaatcccca
      901 ccctgatccg tttcaggtct tctgcaggca tgatttccag caatgatccc atgccgttgt
      961 ggccgccgtc cccgccgcca ttcctcaaac gcagactggc caagggcagt tcgatgtcat
     1021 catggaccac cagggcttcg gagaatttcc atttcctacg cgcttccgcc aacgcttggc
     1081 cagatctgtt catgtaggtg ttgggtttga ttaggcaagc ttcgcggtgg cgaagataat
     1141 cgaactggca gccatgcttg aaagccttgt ggtgtctttt ggcccaaagc tcgagacaga
     1201 ggaagccggc attgtggcgt gagagctcat attccttgcc gatgttcccc agtcccatga
     1261 tcagcttcat tgttcgggct tgggttcctc ggcagcgggt tcttcaccct catcgggagc
     1321 ttcctcggct ttcttgccgt gcacaaccac tagggtgatg tcgtcttggt ctttgtattg
     1381 ccaattgcct gtgggcaagt tccttatatg gagagtgtcc ccgactttga gatcggagac
     1441 gtccagactg atctcttcgg gaacatcctt ggctttgcaa ctgatcttca ccgttctttg
     1501 aatcacatcc aagaatccac cttctttggt gccgatggct tcgcccacaa tttgaatggg
     1561 gatctcgaat tccatttctg cggattcttc taccaccatg aaatcgatgt gcaggatgtt
     1621 gcgggttacg gggtgtacaa gtttgtcctt gaggatggta tggtatttgt tgccattcag
     1681 gactatctcg taaaaagtca gttcgctgaa gctttgcttg tagtactgca gaaacttagc
     1741 gcggtctatc gcaatgggtg tggattcgac cgttttgcca taaagaacag cagggatcat
     1801 tccctgacgg cgcagggcat taatttcgct tttcttgctg gtttgtttgg gttgtgcttc
     1861 gatagtaaat atcacttatt cctccattct caatatctat ttgaacagaa tactgagaga
     1921 ttcctctata tgtactttct tgatggcgat ggccagcatt tccgcaatgg aaagctgcac
     1981 aatcttgggg caactggccg cttccgcgga aagggggatt gtgtttgtga caaagagctt
     2041 ttccagcggc gagttttcaa tgctttgaac aacctttccg gaaagaactc cgtgagtgca
     2101 ggcagcgtag atttttttga catcgtaatt attcaacacc tcggctatct tgaccaagct
     2161 gccgccggtg tcgatgatgt cgtcgaaaag gatggccgtt ttgccggcaa catctccgat
     2221 aatgttcagc aggatggcat ggtcatcatt tccggtgcgg cgtttatcgc caatggccag
     2281 accgcagttg agtctcttgg ccagagcccg ggcacggttg gcgccacctg aatcagggga
     2341 aaccacaaca ggattttcaa tatc
//
LOCUS       DAOOWR010000259         2323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_46096_length_2323_cov_3.798501, whole genome shotgun sequence.
ACCESSION   DAOOWR010000259 DAOOWR010000000
VERSION     DAOOWR010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46096_length_2323_cov_3.798501"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLB85_06285"
     CDS             <1..472
                     /locus_tag="PLB85_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU72974.1"
                     /translation="GRHDHFSKELVPLDALAGQSFWLRFRLVDQSTHIELTDPGWTLD
                     NIRLICGTSTSAQDPGLPGLPQTALYQNYPNPFNPKTTIRYSLAVPNEVRLGIYNLKG
                     QLVKELVREHLPAGDHQSVWDGSDSAGKQVGSGIYLYRLQSGDYSKTLKMILVK"
     gene            complement(476..2254)
                     /locus_tag="PLB85_06290"
     CDS             complement(476..2254)
                     /locus_tag="PLB85_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU72975.1"
                     /translation="MLNKDPEGIKYIADKEGKRYVLKLFFKYKYSDLESIMDLQQRLK
                     RMGKLSSAQTARVAEINQTHDPIFMATEFVSGDSLARIKAYNKERLSENFVREVAKQL
                     VQTAIAVRKQGLSLHELTPSGIMVPEDSNTITVLSSGIKYEEVDERDEVFNLGVIIAQ
                     LLSGNVLYKASYSSENLKLHKFSHIPGVTVSMNKILSDCLHRNILHRNTSLEALLKGL
                     NNLPPVEEDEVFAPKGASQSLAKLQDQPLPLPKHHLEWNFWLIIGLIVAGLALFFIFG
                     LPAIQESGGLVGDIRNIITGTPDTLETNTPDLPQPRQSQNTTSDQQNQGTVREDPRKM
                     NLPGEQTYQTPGTKPARTAPPPDASKFVRVPGGIFGFGRLKDNPNSNTSQDGFWIGKY
                     EVTQSEWEEFMSTPYLYGTRAGNLPVENVSWIDIIRYCNARSEREGLQLAYSITGGAA
                     SVTCNFAANGYRLPTEAEWEMAAKAGTLLDYSGSDDANDVAWHYDNSKLRYRVGGGKK
                     ANSYGIYDMSGNVAEWCWDWYDAKYPSKLLEFNNPRGPSSGSQKVIRGGSVKNGIGTN
                     LGILYRDKGNPNKGLPYVGFRLVRSR"
BASE COUNT          480 a          621 c          645 g          577 t
ORIGIN      
        1 cggccgccac gatcatttca gcaaagaact tgttccgcta gatgcccttg ccgggcaaag
       61 cttctggctg cgctttcgcc tcgtggatca gtcaacccac attgaactta cagacccggg
      121 ctggaccctc gacaatatca ggctgatctg cggaacttcc acctcagccc aggaccccgg
      181 actgccggga ttgccgcaaa ccgcgttgta tcagaactat cccaatccct ttaaccccaa
      241 aaccaccatc cgctacagcc tcgccgtgcc gaacgaggtg cgcctcggca tctacaacct
      301 gaaagggcag ttggtgaagg aactggtgag ggaacatctg cccgcaggtg accatcagag
      361 cgtctgggat ggcagcgaca gcgccggaaa gcaggtggga agcggaatat acctctacag
      421 actgcagagc ggggattaca gtaaaaccct gaaaatgatc ctggtcaaat aggcgtcagc
      481 ggctgcggac cagcctgaag cccacataag gtaaaccttt gttgggattg cccttgtcgc
      541 ggtagaggat gcccaggttg gtgccgatcc cgtttttcac gctgccgccg cgaatcactt
      601 tctggctacc gctggacggg ccccgggggt tgttgaactc caacagtttg cttgggtatt
      661 tggcatcata ccagtcccag caccattccg ccacattgcc gctcatgtca taaataccgt
      721 aggagttggc tttctttccg ccgccgacgc ggtagcgaag cttgctgttg tcataatgcc
      781 aggcaacgtc gttggcatcg tctgacccgc tgtaatccag caatgttccg gctttggccg
      841 ccatttccca ttcggcttca gtgggcaggc ggtaaccatt tgccgcaaaa ttgcaggtca
      901 cggacgcagc cccgccagtt atactgtagg ccagttgtag cccttctctt tcgctgcggg
      961 cgttgcaata gcggatgatg tctatccagc taacgttctc caccggcaga ttgcctgccc
     1021 gggtgccata aagatagggc gtggacatga attcttccca ctcgctttgg gtaacttcat
     1081 attttcctat ccagaaaccg tcctgggagg tgttggaatt gggattgtcc ttcagccggc
     1141 caaagccgaa gattccgccc ggtacgcgca cgaatttgct ggcgtctggc ggaggggcgg
     1201 ttctggcagg tttggtgccc ggggtttgat aggtctgttc tccgggaagg ttcatttttc
     1261 gcgggtcttc cctgaccgtg ccctgattct gttgatcgga ggtggtgttt tgtgattgcc
     1321 gtggctgcgg cagatctggg gtgttggttt ccagggtgtc gggggttcca gtgattatgt
     1381 ttctgatatc tccaaccagc ccgcctgatt cttgaatggc gggcaagccg aagatgaaga
     1441 acagggcaag acctgcgacg atcagaccga taatcagcca gaaattccat tccagatggt
     1501 gcttgggaag tggcagcggc tggtcctgga gttttgccag ggattgcgaa gcccccttgg
     1561 gcgcgaaaac ttcatcctct tccaccggcg gcaggttgtt caaaccctta agtaacgcct
     1621 caagggaggt gttgcgatgt aagatgttac ggtgcaggca gtcggagagg attttgttca
     1681 tgctaacggt gacgccaggg atgtgggaaa acttgtgcag cttcagattc tcggagctgt
     1741 aactcgcttt gtacaataca tttccggaaa gcaattgcgc gatgatgacg cccaggttga
     1801 aaacctcatc ccgctcgtcc acttcttcgt atttgattcc tgatgagagg acggtgatgg
     1861 tgttggaatc ctcgggtacc ataattccgc tgggagtgag ttcatgcagt gaaaggccct
     1921 gcttgcgaac ggctatcgcc gtctgcacca gttgcttggc cacttcacgc acgaagtttt
     1981 cgctcaggcg ctctttgttg taagccttga tccttgccag agaatcaccg ctcacgaatt
     2041 ccgtggccat gaaaatcgga tcatgggtct gattgatctc cgcgaccctg gctgtttgag
     2101 cggaactgag tttgcccata cgtttgagcc tttgctgcag gtccatgatc gattcaaggt
     2161 cggaatactt gtacttgaaa aagagcttga gaacgtacct tttgccctct ttatccgcga
     2221 tgtacttgat cccttctggg tctttgttca gcatccggac tatggtgtaa ccgcaaaaaa
     2281 cgacgcccgg gtccataagc ttgaagacgg cattttcttc tca
//
LOCUS       DAOOWR010000260         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_46122_length_2322_cov_4.147331, whole genome shotgun sequence.
ACCESSION   DAOOWR010000260 DAOOWR010000000
VERSION     DAOOWR010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46122_length_2322_cov_4.147331"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLB85_06295"
     CDS             <1..568
                     /locus_tag="PLB85_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72976.1"
                     /translation="GDPDYHCGSYTVQPYSIPGNNHPFEVELEGSWTSHSFLWQPDFV
                     HFRSLHGHYDEPPHSSYVIEDWLYEGPSIPAESTDMKLNINLWLMSGLPPINSQEAEL
                     VVSDVIITLGIFIEPPDLQISRTDTGQILLNWNEVPNALSYVVLEADAPLPVGDPGWI
                     ELEATSLTSILLEQGAEKKFYCVKAVRE"
     gene            complement(617..1510)
                     /locus_tag="PLB85_06300"
     CDS             complement(617..1510)
                     /locus_tag="PLB85_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU72977.1"
                     /translation="MQEKTFYYLYHKARGASRQQVMEAAKLSAEEYDRLEQSRGEDVR
                     RIQQDLPRAAGIGPDFVRLTRYIYGGSSDQEQGKPCPEAVKTRSGEVIQLPAVERIPA
                     PEISLRQAISQRRSLRKYSDQPLSLEELSFLLWAASWARDFRSGKNIETTFRNVPSAG
                     SRHPFECYLLVNNVSHLAAGLYWYHPLKHSLVSLEESDDIADRVLDGCMGQEMVVRSA
                     VTFILCARPYRAVWRYQQRSYRYLYVDAGHWGQNIHLAAEAVGAGACMVGAFMDEKMN
                     ACLGLDGEEEFVIYVAPVGKK"
     gene            complement(1525..>2322)
                     /locus_tag="PLB85_06305"
     CDS             complement(1525..>2322)
                     /locus_tag="PLB85_06305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU72978.1"
                     /translation="LMERLRAQNISAVINIGGDGSQRISQGLFELSCPVIGVPKTIDN
                     DLSDTDFTFGFQTAVDAATDAVDKLVTTAASHHRVLILEVMGRYAGWIAMHAAIAGGA
                     EVCLIPEIDYDINSVMNALNRRFTAGKGFANIVIAEGAKPLAGTMSYTASEEPGAMPI
                     RLGGAGLRLSQELKDAGCPIEIRETILGHLQRGGRPNSFDRVLASQFGVAAFEMVLQQ
                     KWGYMAAYRHPDIVAVPLAEAIKTYNHVDLKSGLVRTARGLGICLGD"
BASE COUNT          470 a          658 c          703 g          491 t
ORIGIN      
        1 gggcgatccg gactatcact gcggcagcta caccgtccag ccctactcta ttccaggcaa
       61 caaccatcct ttcgaggttg aactggaagg gtcctggact tcgcactcct tcctctggca
      121 gccggatttc gtccatttcc gcagccttca cgggcattat gatgaaccgc cccattccag
      181 ttatgtgatc gaggactggc tctatgaagg gcccagcatc cccgcggaat caacggacat
      241 gaagctgaac atcaacctct ggctgatgag cggactgccg cccataaaca gccaggaagc
      301 ggaactggtc gtctctgatg taatcattac cctgggcatc ttcattgagc cacctgatct
      361 gcaaatctcc agaacggaca ctggccagat cctcctgaat tggaacgaag ttcccaacgc
      421 cctcagctat gtggttttag aggcggatgc gcccctcccg gttggcgatc ccggctggat
      481 cgaactcgag gcaacatctt taacttccat attgctggaa cagggcgcgg agaaaaaatt
      541 ctactgcgtg aaggcggtgc gggaataggg aacacttgga tgagatgaaa acgatagtgg
      601 gctgttcagg cgcggctcac ttttttccca cgggggcgac gtagatcaca aactcctctt
      661 caccgtccaa gcccaggcag gcgttcattt tctcatccat gaaggcgccg accatgcagg
      721 ctccggctcc cacagcctcg gcggcgagat ggatgttttg gccccagtgg ccggcgtcaa
      781 catacagata gcggtaggag cgctgctgat aacgccagac agcgcgatag ggccgcgcgc
      841 agaggatgaa ggtgacagcg gagcgcacca ccatttcctg tcccatgcag ccatccaaaa
      901 cgcgatctgc gatatcgtcc gactcttcca gactgaccaa actgtgtttg agaggatgat
      961 accagtagag cccggcggcg aggtgggaca cattattcac cagcagatag cattcaaagg
     1021 ggtggcggct gccggctgag ggaacgttgc ggaaagtggt ctcaatgttt ttaccgctgc
     1081 ggaaatcgcg cgcccaactg gccgcccaaa gcaggaagga aagctcttcc aaagacagcg
     1141 gctgatctga gtatttgcgc aggctgcggc gctggctgat ggcctggcgc agtgaaattt
     1201 ccggcgccgg aatcctttcc acggcaggaa gttggatgac ctctccggag cgggttttca
     1261 ccgcttcggg acagggtttg ccctgctcct ggtcgctgga gccgccatag atgtaacggg
     1321 tgaggcgcac gaaatccggt ccgatgccgg cagcccgggg caggtcttgt tgaatccggc
     1381 gaacgtcttc cccacgactc tgttctagcc ggtcatattc ctccgcgctc agtttcgcgg
     1441 cttccatcac ttgttgccgg ctcgcgccgc gggctttgtg atagaggtag taaaaagtct
     1501 tttcttgcat gatcgtctcc ttggtcagtc gcccagacag atgcccagcc cgcgcgcagt
     1561 tcgcaccagg cctgatttca gatcaacatg gttgtaggtt ttgatggctt cggccaaagg
     1621 cacagcgacg atatccggat ggcggtaggc agccatgtaa ccccattttt gctgcagcac
     1681 catttcaaag gccgccacgc cgaattgcga ggccagcaca cggtcgaagg aattggggcg
     1741 tccgccgcgc tggaggtggc cgaggatggt ttcgcgaatc tctatgggac agccggcatc
     1801 cttgagttcc tgggagagac gcagccctgc tccgcccagg cggatgggca tggcgccggg
     1861 ttcctcgctg gcggtgtagc tcatcgttcc ggccagcggt ttggcgccct cggcgatcac
     1921 gatgttggca aagcctttgc ctgcggtgaa gcggcggttg agcgcgttca tcacggaatt
     1981 gatgtcataa tcgatctcgg ggatgaggca gacttctgct ccgccggcga tggctgcgtg
     2041 catcgctatc cagccagcgt agcggcccat cacttccagg atcagaacgc ggtgatggct
     2101 ggcggcggtg gtcaccagct tgtccacggc atcagtggcg gcatcgacgg cggtctggaa
     2161 accgaaggtg aaatcggtgt cggagaggtc gttgtcgatg gttttgggta ctccgatcac
     2221 gggacagctg agttcgaaca agccctgtga aatgcgttgg gagccgtctc cgccgatgtt
     2281 gatgacggcg ctgatgttct gggcgcggag gcgttccata ag
//
LOCUS       DAOOWR010000261         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_46133_length_2322_cov_2.679753, whole genome shotgun sequence.
ACCESSION   DAOOWR010000261 DAOOWR010000000
VERSION     DAOOWR010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46133_length_2322_cov_2.679753"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..>2322
                     /locus_tag="PLB85_06310"
     CDS             388..>2322
                     /locus_tag="PLB85_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOU72979.1"
                     /translation="MKYFILTLIMAMGLAVLAADVVFSYTLNEPELKTANGYSHLKLD
                     GAQSWGTPGSPDLPWYGTKILLPIGEEAVDIQVEFSSPQTLTLTLPVAPLQPQYPLSS
                     TVVHPRHQPDPAIYGSTTPYPAMPHNGLNTQFLAGHPISFSAVCPFSYDPVNNELVFY
                     RQFSLRVKTASTARAAAAMQNLKQDTATSAYLQKAVDNTDNLPHYRNRTTGWEYLVIH
                     DEAKLAQWLPFKDLYNARGQNVLLKSVQDITAQTAGADTQEKIRNYIIDVYAANPLRH
                     VLLAGDTDVIPHRGFYVNMGSGGSIDADIPADMYYSCLDGNWNNDGDSYWGEYMEADL
                     VPELSIGRVCYNSDDEIANFITKTQRYLNEPVIAEATTALFAGEWLWEGPTWGGDYMD
                     EMIGGSSANGYTTVGIPSSWNITTLYDRTYGAPDSWTGSQLRPLLSNGPNLVNHLGHS
                     ATTYTMRMTNNYVTSSTITNDGINHNLSVVFTQGCYAGSFDNRETNVGEYTADCITEK
                     FTSISTAAVAMISHSRYGWGVQGSTDGASQKFHRQYIDAIFGEGINELGFALVDSKID
                     NIPYIQNTPVMYWVTYETNLFGDPGLMVWSDTPQQMNVQLPSYWAVGLNSYQIQTNAP
                     GAVLRLKDGTDILCEATADAT"
BASE COUNT          588 a          678 c          536 g          520 t
ORIGIN      
        1 gttcaacgta agcagggatt ttctcccttg gcagcacaaa tgcccgaacc tcctctccgc
       61 ggtccagcaa ctctttaacc acataacttc ccaaatgacc ggctgcgccg gttaccacgt
      121 aaatctgttt catcattcac ctcacattat tgtgctgccg atgtcggcta agttataaac
      181 aaatggattt attaatctgg atcgtccacg gttatccgtg actttgtttc ggttgaacct
      241 gacgatattt tcctatctgt gaacatgtct gataaaagtc aagcagaatc ttcagactct
      301 gccccgtgtc gtgtttcaaa taaaacgggc tttggaatgt catttgcata aacacgatat
      361 agacaaaatc tgaataagtt ggcggatatg aagtatttca ttttgaccct gattatggct
      421 atgggccttg cggtcctagc agccgatgta gttttttcct acaccttgaa cgaaccagaa
      481 ctaaaaaccg caaacggcta cagccatctg aaactggatg gagcccaaag ctggggaacc
      541 ccgggcagcc ctgacctgcc ttggtatggg accaagatcc ttctccccat tggagaagaa
      601 gctgtggata tccaggtgga attcagttcc cctcagacct taaccctcac gcttccggtt
      661 gccccgctgc agccgcagta cccgctttcc agcactgtgg ttcacccaag gcatcagccg
      721 gacccagcca tttacggaag cactacccct tatccggcta tgcctcacaa tggccttaac
      781 actcaattcc tggccgggca cccgatcagt tttagcgctg tttgcccctt cagctacgat
      841 cccgtgaaca acgaactggt cttttaccgc cagttcagcc tgcgggtcaa aaccgcctcc
      901 acagcacgtg ccgcggccgc catgcagaat ttgaagcagg acactgccac ttccgcttat
      961 ctgcagaaag cggttgacaa tactgataat ctgccacatt atagaaatcg caccaccggc
     1021 tgggaatatc tcgtgatcca cgacgaagcc aagctggccc agtggctgcc ctttaaagat
     1081 ttatacaatg cccgggggca aaatgtcctg ctgaaatcag ttcaggatat cacagctcaa
     1141 accgctgggg cagacacgca ggaaaagatc cgcaattaca tcattgacgt ttacgccgcc
     1201 aatcccctcc ggcacgtact gctggccggt gacacagacg tgattcccca ccgcggcttc
     1261 tacgttaata tgggaagtgg cggatcaatc gacgcggata tccccgccga tatgtattac
     1321 agctgcctgg acggaaactg gaacaatgac ggcgacagtt attggggcga atacatggag
     1381 gctgacctgg tgcctgaact atccatcggt cgagtatgtt acaattccga tgacgagata
     1441 gccaatttca ttaccaagac gcagcgctac ctgaacgagc cggtgattgc cgaggccacc
     1501 acagcgcttt tcgcggggga atggctttgg gaaggcccca cctggggcgg cgattatatg
     1561 gatgaaatga taggcggcag ctctgccaac ggctacacaa ccgtgggaat tccttcttcc
     1621 tggaacataa ccaccctgta cgatcgcact tacggcgccc cagattcctg gaccggaagc
     1681 cagctcagac ctttgctgag caacggacct aacctggtta accaccttgg ccattccgcc
     1741 accacataca ccatgagaat gaccaataac tacgtaacct ccagcaccat caccaatgac
     1801 ggcatcaacc acaacctttc cgtggtcttc acccagggtt gctatgccgg ctcattcgac
     1861 aaccgcgaaa ccaacgtggg cgaatatacc gccgactgca taactgaaaa gttcacaagc
     1921 atatccaccg cggcagtcgc catgatatcc cattccagat atggctgggg tgtgcagggt
     1981 tccaccgacg gcgcttcaca aaagttccac cgccaataca tagatgccat cttcggggaa
     2041 gggatcaatg aactcggttt cgctctggtt gattccaaaa tcgacaatat cccctacatc
     2101 caaaacactc cggtaatgta ttgggtaacc tatgaaacaa accttttcgg ggaccccggc
     2161 ctgatggtct ggagtgacac cccgcagcag atgaacgtgc agcttccctc ctattgggct
     2221 gtgggtctga acagctatca gatccagacc aacgctcccg gtgctgtcct caggctgaaa
     2281 gacggcactg acatactctg cgaagccaca gccgacgcaa cc
//
LOCUS       DAOOWR010000262         2285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47151_length_2285_cov_3.935426, whole genome shotgun sequence.
ACCESSION   DAOOWR010000262 DAOOWR010000000
VERSION     DAOOWR010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47151_length_2285_cov_3.935426"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLB85_06315"
     CDS             complement(<1..500)
                     /locus_tag="PLB85_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOU72980.1"
                     /translation="MYIDHRYDVLEILGTGSWANVFKVRDVRTDNIYTLKLFQYLSSQ
                     DLYKQFKAADMHHITKIVHPNLSRVVDFGHVSDHIYFVSEYFDGTTLNNFRFNKARVN
                     DFYDIIVQICYALNALHTQNIMHNDLKPENILYRKTRNGIEVKVIDYGFSRPELSLDT
                     QYVTGTL"
     gene            complement(636..1547)
                     /locus_tag="PLB85_06320"
     CDS             complement(636..1547)
                     /locus_tag="PLB85_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU72981.1"
                     /translation="MKRNNLVNRTEYLLFRFALFKLRLLPYPWGRWLLTRLFLWIGYG
                     LGIRRQVAAENLQRVYPDMPPKQRKKLLKRVYLNLGLSAAELYLLPEKQLISGTSING
                     RENLEAALALGKGVILATAHLGNWEAARTLPLFGIPLAAVVQKQHNHLFDAYNNALRT
                     RQGVRLIDQRHGLKDLLACLRQNMVVTILTDQNAGPEGLILEFLGHPAPHWKGAAKIS
                     LRYKAPIIPGYALRNPEGGIRICFEPMIHHPELSDSTENQLLLLKEINAVTERYIRQN
                     PKQWLWLHRRWLIPERSPLNQAQEIHS"
     gene            complement(1566..1877)
                     /gene="trxA"
                     /locus_tag="PLB85_06325"
     CDS             complement(1566..1877)
                     /gene="trxA"
                     /locus_tag="PLB85_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU72982.1"
                     /translation="MLIELNDTNFETVTQKGIVVVDFWAAWCGPCRMIAPTLEELAVE
                     QPDITIGKLNVDEFPQLAARHGVMSIPTLLFFVNGELKDTSIGAVPKSVLEKKIELLR
                     G"
BASE COUNT          494 a          579 c          631 g          581 t
ORIGIN      
        1 agggtcccgg ttacgtattg ggtgtccaga ctcagttccg gacgggagaa gccatagtct
       61 atgaccttca cctctatgcc gttacgtgtc tttctataaa gtatattctc aggtttgagg
      121 tcattatgca ttatattctg tgtatgaagg gcgttcaaag cgtagcagat ttgcacgatg
      181 atgtcataga agtcgttcac ccgcgccttg ttgaagcgga agttgttaag cgtggttccg
      241 tcaaagtatt cgctcacgaa gtagatatga tcgctaacgt gtccgaaatc aactacccgg
      301 ctgaggttgg gatgcacgat cttggtgatg tggtgcatgt cggcagcctt gaactgcttg
      361 taaaggtcct gcgacgagag gtattggaac agcttcaggg tgtaaatgtt atccgtgcgg
      421 acgtcacgca ccttgaaaac gttggcccag gaccccgttc ccaagatttc cagaacatcg
      481 tagcggtggt ctatgtacat ctttgaactc ccaaaatgat tggttttacc ctaatttcac
      541 agtggcagag cttgtcaacc gaaacttgac gattccggct ggtttttgca aggaggagct
      601 tggagtcgag aaccacaaac ggcttctgga cagcttcagg agtggatttc ttgcgcctgg
      661 ttcagggggc ttcgctctgg aatcagccag cgccgatgca gccagagcca ctgcttcgga
      721 ttctgcctga tgtagcgttc ggtgacggcg ttaatctctt tcaggaggag gagttggttt
      781 tccgtgctgt cactcagttc cggatggtgg atcatcggtt caaagcagat gcggatgccc
      841 ccttcagggt ttcgcaatgc gtagcccgga atgattggag ccttgtagcg cagcgagatt
      901 ttcgccgccc ctttccaatg cggagccggg tggcccagaa attccagaat cagtccctca
      961 ggaccggcat tttggtcggt gaggatggtc accaccatgt tttggcgcag gcaggccaga
     1021 aggtctttca gcccgtgccg ctggtctatg agccgcactc cctgacgggt tcgcaaggcg
     1081 ttgttgtagg cgtcgaacag gtgattgtgc tgtttttgca ccaccgcggc cagaggaatg
     1141 ccgaagagcg gcaaagtcct cgccgcctcc cagttgccca ggtgggctgt ggccagaatt
     1201 actccctttc ccagcgccag tgcggcttcc aagttttccc tgccgtttat gctggtgccg
     1261 gagataagct gcttttccgg caggaggtaa agctccgcgg cgctgaggcc aagattgagg
     1321 taaacccttt tcaaaagttt ctttctctgc ttgggcggca tatccggata caccctttgg
     1381 aggttttctg ccgccacctg gcgtcgaatg cccagtccgt agccaatcca gaggaaaagg
     1441 cgcgtgagca gccagcgccc ccaagggtag ggaagcagcc tcagtttaaa caaagcgaac
     1501 cggaagagca gatactctgt ccggttcacg aggttgttac gcttcaaact cagcctgggt
     1561 gacaatcagc cgcgcagtag ttcgattttt ttctcaagca ccgatttggg cacagccccg
     1621 atggaggtgt ccttgagttc accattcacg aagaacagca gggtggggat gctcataacg
     1681 ccatgacggg cggcaagttg gggaaattca tccacgttca gctttccgat ggttatgtcg
     1741 ggttgctcaa ccgccagttc ctccagcgtt ggcgctatca ttctgcaggg accgcaccag
     1801 gcagcccaaa aatccacaac tacaatgcct ttttgggtca cagtctcgaa gtttgtatcg
     1861 ttcaactcta tcaacatgtt tggctcctgt catttttatc ttttgatata ataatccagc
     1921 gtatctcctg cgcaagtggc ttgctttgac cgggttctga aacacggaga agttacctgg
     1981 catacggcga acacggtgag catggacgac aggatgtatt aaagtataaa tactttgctc
     2041 ttttctgatg tcgatgctca ttcctcgctc gacgccagcg tgttgccttg cgatgccttt
     2101 aggccattaa ggccctgtat cgtgatccta aagcgcctcc cgccaaatac ccgcatttga
     2161 tgcgggaatt atacgagaga tgggggaggg cgatagtcgc gagggctaag ggcgtggctg
     2221 acaggacaag agagcctctc cattccgggc cttcccgtct taacatattc gcgctcagat
     2281 ataaa
//
LOCUS       DAOOWR010000263         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47229_length_2282_cov_4.711271, whole genome shotgun sequence.
ACCESSION   DAOOWR010000263 DAOOWR010000000
VERSION     DAOOWR010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47229_length_2282_cov_4.711271"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLB85_06330"
     CDS             <1..758
                     /locus_tag="PLB85_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOU72983.1"
                     /translation="NMLRSQERSPLLELCRKLRDARVGESEERKPRYSWNLKVVGGDV
                     VMQREDNLSPSRFVVEELMILYNRLMAERACQSGLPLIYRNVTQWSEDDNDTDNYGIQ
                     AYLSTEAKFHPGVGVQAYLHATSPIRRFTDIVNQAQFSALLAGNGQCFSRQDLEELIF
                     SIEKRLQLLRAVAHRSERYWLLRWLEQKHLGEPLDAVPLRRVKQGWLMELSRWEKRLV
                     LCCEEDLPLRASVKLVVASVDMKELLVRGDIIL"
     gene            1137..>2282
                     /locus_tag="PLB85_06335"
     CDS             1137..>2282
                     /locus_tag="PLB85_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU72984.1"
                     /translation="MQATWRKLGFRCGLEVHQQLNTSQKLFCRCPAGKYHDFDDFDAE
                     IVRHMRPTLSELGEYDGTALMEFKTRKKIIYRINNDSACTYEVDDTPPFPLNRQALKQ
                     AMKIALLLKMKIVGELHITRKQYLDGSIPTGFQRTTILGIEGEIPISNKTIKVIQFSV
                     EEDSCREVSDFRHTRVYFADRLGMPLIETVTYPDMETPWEAAEAAQNIRFIARSSGLV
                     RTGIGAAREDVNVSIEGGTRVEIKGVAHISWIPKLTHNEAFRQKSLLLVREELRKRVA
                     DPAKWKLTHTFLDPADWRHIPLLKKAGSDGWKLIAVNLPHFAGILSFFNQPGRCFADE
                     ISDRLKVIACLERPNMFHSEEIAGFEQGADSDLSKMENRISDKPLVQA"
BASE COUNT          534 a          619 c          624 g          505 t
ORIGIN      
        1 acaatatgct gcgctcccag gaacgctcac cgctgttgga actctgccgc aaacttcgcg
       61 acgccagggt gggggagagc gaggaacgta agcctcgcta cagctggaat ctaaaggttg
      121 ttggcggtga tgttgtaatg cagcgggagg ataatctcag cccgtcacgg ttcgtagtcg
      181 aagagctgat gatcctctac aaccgcctga tggccgagcg cgcctgccaa agtggcctgc
      241 ccttgatcta ccgtaatgtg acccagtggt ccgaagacga caatgataca gataattacg
      301 gtatccaggc ctatctctcc accgaggcaa aattccaccc cggagtgggc gtccaggctt
      361 atctgcacgc cacttcaccc attcggcgct tcactgacat cgtgaaccag gctcagtttt
      421 ctgctttact ggcggggaat gggcaatgct tttcgcgcca ggaccttgag gaactgatct
      481 tcagcatcga aaaacgcctg caactgctac gggctgtcgc ccatcgcagc gaacgctact
      541 ggctgctgcg ctggctggag caaaaacacc ttggcgagcc gcttgatgcg gtgccgctgc
      601 gccgggtgaa acaaggctgg ctgatggagc tcagccgctg ggaaaaacgg ctggtgctgt
      661 gttgcgagga ggatttgccg ctgcgggcgt cggttaagct ggtggtggca agtgtggata
      721 tgaaggagtt gttggttcgt ggagacatta tactctgagc agctcggggg ctggtgtctc
      781 aatcgattat aaacctttcc ggcctggatt ccaggagcct tgtgatgaag ttcaacccca
      841 ttcagacata tagagtctga gaaacctgat aatctgcttt gttccaaatc atacaagcct
      901 ttcattgagc ctaattcaat gggacttggc ggcttaggtt caagctggcg gaaaacggct
      961 tcctgaggct ttttgcagcc ttgattcagg gtgtgaaata atgctggaca aaaaaggcac
     1021 acctctggat tatggtctat cacattacat attagggacc ttaattggac aaccctcacc
     1081 aacgaaattg gctgcttagc cgggcaaaca ccggctgggt cagccttccg gacgccgtgc
     1141 aagcgacctg gcggaagctt ggtttccgtt gcggtctgga agttcaccag caactgaaca
     1201 cctcccagaa actcttctgc cgctgccccg caggcaagta tcacgatttt gatgattttg
     1261 acgccgaaat cgtgcgtcat atgaggccca ctttgtctga attgggcgag tatgacggca
     1321 ccgccctgat ggaattcaag acgcggaaaa agataatcta ccgcatcaac aacgacagcg
     1381 cctgcaccta cgaagttgac gacacccctc cttttccgct gaaccggcag gctcttaagc
     1441 aggcaatgaa gatcgccctg ctgctgaaaa tgaagatcgt aggcgaactc cacatcaccc
     1501 gcaagcagta tcttgacggc agtatcccca ctggatttca acgcactacc atcctcggca
     1561 tcgagggaga aattccaatc tccaacaaaa ccatcaaagt gatccagttt tccgtggagg
     1621 aagacagttg ccgcgaagtg tccgattttc gccatacccg ggtttatttt gccgaccgcc
     1681 ttggcatgcc gctgatcgaa acggtcacct atccggatat ggaaacgccc tgggaggcgg
     1741 ctgaggcggc tcagaacatc cgctttatcg cccgcagttc cggtctggtg cgcaccggga
     1801 taggcgcggc ccgcgaggat gtgaatgttt ccattgaagg cggcaccagg gtcgagatca
     1861 agggcgtggc tcacatctcc tggatcccca aactcacgca caacgaggct ttccgtcaaa
     1921 aatcgctact gctggtccgc gaggaactgc ggaaacgggt ggccgacccg gcgaaatgga
     1981 agctgactca tacttttctg gacccggccg actggcgcca catccctctg cttaaaaagg
     2041 ccggaagcga tggctggaag ctgatcgcgg tcaatttgcc ccattttgcc ggcatcctca
     2101 gcttctttaa ccaacccgga cgctgtttcg cagatgagat ctctgaccgt ctgaaagtga
     2161 tcgcctgcct ggaacggcct aatatgttcc attctgaaga gatagctgga tttgaacaag
     2221 gcgcggacag cgatttgagc aaaatggaga accggatttc tgacaaaccc ttagtccagg
     2281 ct
//
LOCUS       DAOOWR010000264         2282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47238_length_2282_cov_3.097890, whole genome shotgun sequence.
ACCESSION   DAOOWR010000264 DAOOWR010000000
VERSION     DAOOWR010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47238_length_2282_cov_3.097890"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /gene="mutL"
                     /locus_tag="PLB85_06340"
     CDS             <1..1768
                     /gene="mutL"
                     /locus_tag="PLB85_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU72985.1"
                     /translation="VENGGKDLIQVSDNGSGMSPDDALLAFERHATSKIRTVGDIISI
                     STLGFRGEALPSIASVSNLTLITRDASSEVATQVDFDFGRLRNVGQTSSNPGTNITVR
                     GLFKALPARRKFLRSATTELRHILKYIHYQAVLHPGIGLKLFVDGNQKLSFVIAENRE
                     QRMGEMFGSGFFNDDIIPIKAENEGYRLEGYIFGLEDRSDKLVDIQYCFINGRFINDK
                     TVKHAIKAAYEPFILKTRVWQKGTTPPYVLFLDIPMEQIDVNVHPAKLEVRFREQQKV
                     HSLVFLTLTEALNDYEHAKFASARVKFDRAQQVNEATFVERTVYKNKVEVPRFSEYKK
                     EFGNLYQDELFPATPGEPVPPGKIQVPLVDMDKKDGEEQLEIADDLPNESFQYKLLLR
                     NEEDYINPWQLHNTYIFIQVEDGLVIIDQHAAHERIIYEKLLQRTQGAPPVRQKLIIP
                     LVIDIPPIIASEIRDLVEQNLELLEKTGFVLKKFSGDSLVIEEIPAELDDWQGGKIFI
                     EILKQLEEEMELNSDFRDSLAKSIACKASIKAGKKLTRKEMLNLINQLFACQMPYFCP
                     HGRPLILKMTLTKFEKKFKRQL"
     gene            1765..>2282
                     /gene="miaA"
                     /locus_tag="PLB85_06345"
     CDS             1765..>2282
                     /gene="miaA"
                     /locus_tag="PLB85_06345"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU72986.1"
                     /translation="MIPLITIEGPTASGKSDLALKLARELETEIISADSRQVYRYLDI
                     CTAKPSTAEMKAVRHHLISIIEPSKSYNAGRFKSDASEVIRDLHRQGMVPLICGGTGL
                     YVEALLQGLFPQIDIDPEIRQRLRERLKKEGLDILYAELAERDPAFAEKVSSNDRQRI
                     LRGLEVFAATGLP"
BASE COUNT          585 a          629 c          592 g          476 t
ORIGIN      
        1 tgttgagaat ggcggcaagg acctgatcca ggtcagcgac aacggcagcg gcatgagccc
       61 cgacgacgcc ttgctggcct ttgaaagaca tgccaccagc aagatcagga ccgtgggaga
      121 catcatcagc atcagcaccc tgggcttccg cggcgaagca ctgcccagca tcgccagcgt
      181 tagcaacctg actctgatca cccgcgatgc atccagcgaa gtcgccaccc aggttgattt
      241 cgatttcgga cgtctgcgca atgtgggaca aacctcctcc aaccccggca ccaacatcac
      301 agtgcgcggc cttttcaagg ctttgcccgc ccggcgaaag tttctccgca gcgccactac
      361 cgaactgcgc cacattctca aatacatcca ctatcaggcc gtgctgcatc ccggcattgg
      421 cttaaaactg tttgtggacg gcaatcagaa gctttccttt gtgatagcgg aaaaccggga
      481 acagaggatg ggggagatgt tcggctctgg atttttcaat gacgacatca ttcccataaa
      541 agccgagaat gaaggctacc gcctggaagg atacatcttc gggctcgaag accgcagcga
      601 caaacttgtg gacatacaat actgcttcat caacgggcgc tttatcaatg acaagacggt
      661 aaaacacgcc atcaaggctg cctacgagcc cttcatcctc aaaacccgcg tctggcaaaa
      721 aggaaccaca ccgccctacg tgctgtttct ggacatcccg atggagcaga tcgatgtgaa
      781 tgtgcatcct gccaaactgg aagtgcggtt ccgggaacag cagaaggtgc attccctggt
      841 ctttcttacc ctcactgagg ccttgaatga ctatgaacac gcgaagtttg cctccgccag
      901 ggtgaaattc gaccgcgccc agcaggtgaa tgaagccacc tttgttgaac gcaccgttta
      961 caaaaacaag gtggaagttc cccgcttttc cgagtataag aaagaatttg gtaacctcta
     1021 tcaggatgag ctgtttcccg ctaccccggg agaacctgtc cctcccggca agatccaggt
     1081 tccgctggtg gatatggaca agaaggacgg cgaggaacag cttgagatag ccgacgacct
     1141 gcccaacgag tcttttcaat acaagttgct gttgcgcaat gaggaagatt atatcaaccc
     1201 ctggcagctg cacaacacct atatatttat ccaggtggag gacggcctgg tgataatcga
     1261 ccagcacgcc gcccacgaac gcatcatcta tgaaaagctg ctgcagcgta cccagggagc
     1321 gcctccagtg cgccagaaac tcatcatacc gttggtgatc gacattccgc cgattattgc
     1381 cagcgaaata cgcgacctgg ttgagcagaa tctcgaactc ttggaaaaga cgggtttcgt
     1441 gctgaagaaa ttcagtggcg actctttggt tatagaggaa ataccagctg aactggacga
     1501 ctggcagggc ggcaagattt tcatcgaaat cctcaaacag ctggaggaag agatggaact
     1561 gaactccgat ttccgcgatt ccctggccaa atcaatcgcc tgcaaggctt ccatcaaggc
     1621 tgggaagaag cttaccagaa aggaaatgct gaacctgatc aatcagcttt tcgcctgtca
     1681 gatgccctat ttctgtcctc atggccggcc tttgatcctg aaaatgaccc ttacgaagtt
     1741 cgaaaagaaa ttcaaacgcc agctatgatc cccctgatca cgattgaggg gcccacggcc
     1801 tcgggaaaat ctgatctggc cctgaagctg gcccgggaac tggaaacaga gatcatttcg
     1861 gcggattcgc gccaggttta ccggtatctg gatatctgca ccgctaagcc ctccactgct
     1921 gagatgaaag ccgtaagaca ccatctaatc agcatcatcg aaccttcgaa gagctacaat
     1981 gcagggaggt ttaaatctga tgcttcggag gtgatacgcg accttcatag gcagggaatg
     2041 gtcccgctga tctgcggcgg caccggactc tatgtggaag cattgctgca gggcctcttt
     2101 ccccagatcg acatcgatcc ggagattcgt caacgcctgc gggagcggct gaaaaaagag
     2161 ggtttggata ttctttacgc ggaactggct gagcgagacc ccgcctttgc agaaaaggta
     2221 agcagcaacg acaggcaacg cattcttcgg ggcctggagg tttttgccgc gacagggctt
     2281 cc
//
LOCUS       DAOOWR010000265         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47258_length_2281_cov_3.756065, whole genome shotgun sequence.
ACCESSION   DAOOWR010000265 DAOOWR010000000
VERSION     DAOOWR010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47258_length_2281_cov_3.756065"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="ftcD"
                     /locus_tag="PLB85_06350"
     CDS             complement(<1..786)
                     /gene="ftcD"
                     /locus_tag="PLB85_06350"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOU72987.1"
                     /translation="MKLMECVPNFSEGRDKTVLEAIASAIRSVKNAVLLDMDPGADTN
                     RTVFTLAGEPEAVVEAAFQAIRKASELIDMSKHHGEHPRMGATDVCPFIPISGMTMEE
                     CAEYARKLGKRVGEELGIPVYLYENAASKEEWRNLANVRSGEYEALPQKAQDPAWKPD
                     FGPHAFNPRSGATAISAREFLIAYNINLNTRDKKKAHALALTIRERGKPARDEAGKLI
                     RDADGNRVIIPGLFSHCKAVGWYIDSYDRAQISINLTNYKITPP"
     gene            complement(783..2057)
                     /locus_tag="PLB85_06355"
     CDS             complement(783..2057)
                     /locus_tag="PLB85_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU72988.1"
                     /translation="MDKLSVSEILLWSLIFLVLLGLAFLFAGFENGIISINMLELEGK
                     ARKDKSSAKLLASLRKPDKLLGTSLIGNNVVIILMSTLSTYVASRYITGFDARYTSLV
                     VGTVVLVFGEVVPKSIFRDHADTLVPVVYPFMRFVYFLFKPLVMGISWLNSVLRKWLK
                     ITEGNNFNYLTKDDLSYLLSLTEADEADEPQIEMIEDALDFTEQVARNVMVPRTELVA
                     IPETATVAEAIEIAKEEGYTRYPVFGKDLDDIKGVLIIYDALKREFTPDTVVSKLIHK
                     PFFTPENTDLDVLLREMQKNHRSIAIVVDAYGGTSGIVTMEDILEEIFGDIADEYDEA
                     EEIPEVEQVSPNTWLVQADIEIDHLADEYEIILPEGDYETVAGLILDRLARIPHQGQI
                     VNVDAYRIQVLQATDKKIIKVKIHRTSTRGES"
     gene            complement(2050..>2281)
                     /locus_tag="PLB85_06360"
     CDS             complement(2050..>2281)
                     /locus_tag="PLB85_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU72989.1"
                     /translation="DAEISRQEQEIARSRKAQVSTGDRSAKIRTYNFPQSRVTDHRIN
                     LTSYNLDSFLSGNIDEFIQALRIAWRNEKVNG"
BASE COUNT          492 a          553 c          631 g          605 t
ORIGIN      
        1 gggcggggtg attttataat ttgtgaggtt gatgctgatc tgggcgcggt cgtagctgtc
       61 gatataccat cccacggctt tgcagtggct gaagaggccg gggatgatta ctctgttgcc
      121 atcggcatcc ctgattagtt tgccagcctc gtcacgggct ggtttgcctc tttcgcggat
      181 ggtgagcgcc agggcgtgag ctttcttttt atcccgggtg tttaaattga tgttgtaggc
      241 gatcaggaat tcacgggcgc tgatggcggt ggcgccggaa cggggattga aggcgtgggg
      301 accgaaatcc ggcttccaag cagggtcctg ggctttttgg ggtagggctt catattcgcc
      361 ggagcgcaca tttgccaggt tgcgccactc ttctttagaa gcggcatttt cgtaaagata
      421 taccgggatg ccgagttctt cgccgaccct ctttcccagt ttgcgtgcgt attcggcaca
      481 ttcttccatg gtcattcccg agatcgggat gaaggggcaa acgtcagtgg cgcccatgcg
      541 gggatgttca ccatggtgtt tgctcatatc gatcagctcg gaggctttcc tgatggcttg
      601 aaaagcggct tccacaacgg cttctggttc tccggccaaa gtgaaaacgg ttcggttggt
      661 gtcagcgccg ggatccatat caagtaaaac agcattctta acagagcgaa tagcggacgc
      721 gatggcttcc agaacagttt tgtccctgcc ttcgctgaaa ttggggacgc attccataag
      781 tttcatgatt cacctcttgt actggttctg tgtattttaa ctttgataat cttcttgtct
      841 gtggcttgca gcacctgaat gcggtatgca tccacattca cgatctggcc ttggtgtggg
      901 atgcgggcca gtctgtccag gatcagccca gccacagttt cgtaatcgcc ctcaggaagg
      961 atgatttcat attcgtcggc cagatggtcg atttcgatat ccgcctggac cagccaggtg
     1021 ttgggggaaa cctgctccac ttccgggatt tcctcagctt cgtcatattc gtccgcgatg
     1081 tcgccaaaga tctcttccaa aatgtcttcc atggtcacga tccctgaagt gccgccgtag
     1141 gcatcaacca cgatggcaat gctgcgatgg tttttctgca tttcgcggag caacacgtcc
     1201 agatcggtgt tttccggagt gaaaaagggt ttgtggatga gtttgctgac cacggtgtcc
     1261 ggtgtgaatt cacgtttgag cgcatcatag attatcagca cacccttgat atcgtcaaga
     1321 tcttttccga acacagggta gcgtgtgtag ccttcctcct tggctatctc tatggcctct
     1381 gcaaccgtcg cggtttcagg gatggcaacc agttccgtgc gcgggaccat aacgttgcgc
     1441 gccacctgtt cagtgaaatc cagcgcgtct tcaatcattt ctatctgcgg ctcgtcggct
     1501 tcatcagcct ccgtgagcga tagcaggtag gagagatcgt ccttcgtgag atagttgaag
     1561 ttattccctt ctgttatctt cagccatttg cgcagaaccg agttcagcca ggaaataccc
     1621 atgaccaggg gtttgaaaag gaagtaaacg aaacgcatga aaggataaac aaccggtacg
     1681 agagtgtcag cgtgatcccg gaagatggat ttggggacaa cttcaccgaa tacaagcact
     1741 actgtgccaa cgaccaagga cgtgtagcgc gcatcaaagc cggtgatgta tctgcttgcc
     1801 acataggtgg aaagggtgga catgagaata atcacgacgt tgtttcctat caggctggtg
     1861 cccaaaagct tgtccggttt gcgcaagctg gcgagcagct tggccgagga tttatccttt
     1921 ctggcctttc cttccagttc gagcatattg attgagatga taccgttttc aaacccggca
     1981 aaaaggaatg ccaatcccag aaggaccaag aaaatcaagc tccagagaag tatttcagag
     2041 acagacaatt tatccattaa ccttttcgtt gcgccaggcg atccgcaggg cctggatgaa
     2101 ctcatcaatg ttgccagaca aaaaagaatc aagattgtag cttgtcaagt ttattctgtg
     2161 gtcggtgacc cgggattggg ggaaattgta ggtgcggatt ttagcggaac ggtccccggt
     2221 gctgacttgg gccttgcgtg agcgggcgat ttcctgttcc tgcctgctga tttcagcatc
     2281 g
//
LOCUS       DAOOWR010000266         2281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47269_length_2281_cov_2.792453, whole genome shotgun sequence.
ACCESSION   DAOOWR010000266 DAOOWR010000000
VERSION     DAOOWR010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47269_length_2281_cov_2.792453"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..856)
                     /locus_tag="PLB85_06365"
     CDS             complement(143..856)
                     /locus_tag="PLB85_06365"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOU72990.1"
                     /translation="MIKVTKNTNLLVLTGAGISAESGLRTFRDSDGLWENHRVEDVAT
                     PEAWHRDPKLVWRFYKARWEQSKQAAPNPGHLALARLEDWLGDNFHLITQNVDGLHTA
                     AGNTRVIEMHGALSSCLCTKCGVRLHLDEVDMSPELPACPKCTGRLRPDIVWFGEMPY
                     RLPEIDILLQNCDVFLVVGTSGVVYPAAGFVQMARYLGAKTISVNLHPSEGLGFFDEF
                     HQGRAGEILPRLVDKWMAE"
     gene            complement(853..>2281)
                     /locus_tag="PLB85_06370"
     CDS             complement(853..>2281)
                     /locus_tag="PLB85_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU72991.1"
                     /translation="FVDQTGPGTVTLVSNSPNLLVLSGSYEFDALAASDSTEINGFFQ
                     VKVQGNYSDHQIAYLNFSASFDSYISNTPAALILNAPELHLDTYQFVNANPQVLPGDN
                     PALNMIVSNRGSGHAFSPMLILFCDDPNVTLSAFEATLTPVGPDSFTLYESAFSVLVS
                     PDAPIGSSVNIGYMLGAENGNTLEGNFVLYIGNQQYTFENDSNGWESLAPLTSFVNQW
                     HRESYRNNTAEGSWSMKFGGPGSGAYSSSAYGALESPVIPLGKKGRLYFHHWMDAEVH
                     TIPSYAWDGGLVQISIDEGPWIQITPVGGYPRRIYNNPASPFPANTEVYSGSFGWSPA
                     EFDLSSYTGMAKFRFIFGADGYVTGEGWYIDDVYLESEFDVASEDLVTNLAFNLHGNY
                     PNPFNPTTTICFDLPKAATVNLDIFNLRGQRVRSLVNTDLPSGTHNAVWNGRDDAGSP
                     VSSGVYLYRLSNGEREITRRMVLMK"
BASE COUNT          502 a          568 c          705 g          506 t
ORIGIN      
        1 gggacagggc cgaaagaaag ggccttaacg gtcaggggac acggacagaa caaacgctgg
       61 aattgagcaa gaaaaaaggc ggtatggaag ccgccttgat aagttagccg tgccaggatt
      121 cacccgagcg acggtttgga atctattccg ccatccactt gtccaccaaa cggggcagga
      181 tttcgcccgc ccggccctgg tgaaattcgt caaagaaacc aagcccttcg gatggatgga
      241 ggttcaccga aatggttttt gcccccaagt agcgggccat ttgcacaaaa ccggcagcgg
      301 gatagaccac tccgctggtc cccaccacca ggaaaacatc gcagttttgc agcaggatgt
      361 ctatttccgg caatctgtag ggcatctcac cgaaccagac aatgtccggt cgcagcctgc
      421 cagtgcattt gggacaggcc ggcagttcgg gactcatgtc cacttcgtcc agatgtagcc
      481 tgaccccgca ctttgtgcag aggcaactgc tgagagcgcc gtgcatttcg atcacgcggg
      541 tattgccggc agcggtgtgg agaccgtcaa cgttctgggt gatgaggtgg aaattgtcgc
      601 caagccaatc ctccagcctg gccagggcta ggtgtccggg gttgggagcg gcttgtttgg
      661 actgttccca gcgagctttg taaaagcgcc aaaccagttt tgggtcacga tgccaggctt
      721 cgggagtggc aacatcctcc acgcggtggt tttcccaaag gccgtcgctg tcgcgaaagg
      781 tgcgaaggcc cgattcggcg ctgattcccg ccccggtgag gacgagtagg tttgtattct
      841 tcgtgacttt gatcatttca tcaataccat cctacgggtt atttcacgtt cgccattgct
      901 gaggcggtat agatagacac cgctggatac ggggctgccg gcgtcgtcgc gtccgttcca
      961 gacggcattg tgggtgccgg agggcagatc ggtgttcaca agtgaccgca ctctttggcc
     1021 gcgaaggttg aagatgtcca gattcacggt tgccgccttc ggcaaatcga aacagatcgt
     1081 ggtggtggga ttgaaggggt tggggtagtt gccgtgaagg ttgaaggcaa ggtttgtcac
     1141 caaatcctct gaagcgacat cgaattcgct ttccaaataa acatcgtcga tataccatcc
     1201 ttccccggta acatagccgt ctgcgccgaa tatgaagcgg aatttggcca tgcccgtgta
     1261 gcttgaaagg tcaaattcag ccggggacca gccgaaactg ccagaatata cttcagtgtt
     1321 ggccgggaag ggggaagcgg ggttgttgta gatgcggcga ggatatcctc cgacgggggt
     1381 gatctggatc cagggccctt catcgatgga tatttgcacc aggcctccat cccaggcgta
     1441 gcttgggatg gtgtgcactt cggcatccat ccagtggtgg aagtagaggc gccccttctt
     1501 gccaagcggg attaccgggc tttccaaagc tccgtaggca gagctggaat aagctccaga
     1561 accgggtccg ccgaacttca ttgaccagct gccttcagca gtgttgttgc gatagctttc
     1621 acggtgccac tggttcacga aactggtcag gggagccagg ctttcccagc cgttggagtc
     1681 gttttcgaaa gtgtattgct ggttgccgat gtatagcacg aaatttcctt ccagggtgtt
     1741 tccattttcg gcgccgagca tgtagccgat gttcacagaa ctgccgatgg gggcgtcggg
     1801 ggaaaccaaa acgctgaagg cgctttcata gagggtgaaa ctgtcgggcc caactggtgt
     1861 gagcgtggcc tcgaaagcgg acaatgtgac gttggggtca tcacagaaaa ggatgagcat
     1921 ggggctgaag gcgtggccgg aacccctgtt gcttacgatc atgttcaaag caggattgtc
     1981 accgggcagc acctggggat tggcattcac gaactggtag gtatccagat gcagttcagg
     2041 agcgttcagg atcagggcgg ccggtgtatt ggaaatgtag ctgtcgaagc tggctgaaaa
     2101 gttcagatag gcgatctggt ggtctgagta attgccttgg accttaacct ggaagaaacc
     2161 gtttatctct gtggaatcgg aggcggcgag ggcatcgaat tcgtagctgc cgctcagcac
     2221 aagaaggttg ggcgaattgc ttaccagggt gacggttccc ggtccggtct gatctacaaa
     2281 g
//
LOCUS       DAOOWR010000267         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47533_length_2272_cov_2.939558, whole genome shotgun sequence.
ACCESSION   DAOOWR010000267 DAOOWR010000000
VERSION     DAOOWR010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47533_length_2272_cov_2.939558"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLB85_06375"
     CDS             complement(<1..469)
                     /locus_tag="PLB85_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU72992.1"
                     /translation="MDTDNRIGFHAGVVAQYPIGKYLVLQPELMYTQKGWSWTVDMPI
                     VDDIVTTYKLDSVELPILLKMNLDFGSVAIQPFVAPQLSYCVIAERHVNDNVFKIMDD
                     INPFQYGAQFGLDLQLNKSMLIGGRYQMGFSDIFDDDNDNVSYTHNGILISFGY"
     gene            complement(840..1415)
                     /locus_tag="PLB85_06380"
     CDS             complement(840..1415)
                     /locus_tag="PLB85_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU72993.1"
                     /translation="MKKTLIILAVLLVLAGAAQAKLNFGLRSGLNVSKLELSPDGSID
                     TRSALGLHIGGFVQLKTDSPLLIQPELLYTQKGTGTNVDDVVTIDYIAMPVLLKLNVM
                     IPGAKDIYLQPLIAPEMGYAIKATSTYNPLFHESINKLNAGFNLGADLIYTENYLLGL
                     RYYLGMTDLLSSAKRPPISNTCWSISVGYIF"
     gene            1623..>2272
                     /locus_tag="PLB85_06385"
     CDS             1623..>2272
                     /locus_tag="PLB85_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOU72994.1"
                     /translation="MQISELLALLGSKAPASLALEWDNVGLLLGDGSRDFHKALITLD
                     VTPNAVDKALEIGADLILSHHPLIFRPLKRFGDPLLLKLAENRIAVICLHTNLDVAAD
                     GVNQALAAKLGLRVTGLLNSEQGGKWHHLSVTVPIGHTNKVAEAVFAAGGGRIGNYSS
                     CSTRHRITGTYESGEGAKPFIPGDPGQPRTTVSEEELEFMVDEASLGPVIAAVKSV"
BASE COUNT          490 a          580 c          669 g          533 t
ORIGIN      
        1 gatatccgaa gctgatcaag ataccgttgt gagtgtagga aacgttgtcg ttgtcatcgt
       61 caaagatatc cgaaaagccc atctggtagc ggccgccgat cagcatactc ttgttgagct
      121 gcaggtccag accaaactga gcgccgtact ggaagggatt gatatcgtcc atgattttga
      181 acacgttgtc gtttacatgt ctctccgcga taacgcagta actgagctga ggggccacga
      241 aaggctggat ggccacgctg ccaaagtcca ggttcatctt cagcaggatg ggaagttcca
      301 cggaatccag tttgtaagtg gtcacgatgt catccacgat cggcatatcg acggtccagc
      361 tccaaccttt ctgggtgtac atcagttccg gctgcagaac aagatatttg ccaatgggat
      421 actgtgccac cacgcccgcg tggaaaccga tgcggttgtc ggtgtcgatg tcgttgctgc
      481 cgctataagc gcggttggtg aggttgaggc cggcgcgcag ccaaaggctt cgttcgcgta
      541 agcaaggctc aggcctaaca cggccagtag cataactaca aggattttct tcattttggg
      601 tctcctgttt attgttagtt ttatagtagc ttgtgttttt cgctcaatac taaagagctg
      661 cctgaaaatg tcaagaaata attttccgcc ttatgcgaac ctgtgacggg atatagagaa
      721 ctgattcttt ggtgtcccgc aatacattag ctttccgtcg tgaatatccc ggccttccct
      781 ccggcggtca ttacgccgcg atgatactgg tagcgaaggg ggaaaacggg tcgggttttt
      841 tagaatatgt aacccacgct gatagaccag caggtgttgg agatgggggg acgtttggcg
      901 ctgctgagca ggtcggtcat gcccagatag tagcgcaggc ccagcaaata gttctcggtg
      961 tagatcaggt cggcgccgag gttgaaaccc gcgttgagtt tgttgatgct ttcgtggaag
     1021 agcggattgt aggtggatgt cgctttgatg gcgtagccca tttccggcgc gataagcggt
     1081 tgcaggtaaa tatccttggc gcccgggatc atcacgttga gcttgagcag cacgggcatg
     1141 gcgatgtagt ctatggtcac cacatcgtca acgttggtgc cggtcccttt ttgagtgtag
     1201 aggagttccg gctggatgag gagcggggaa tccgttttga gctggacaaa gcctccgata
     1261 tgcagtccca aagcgctgcg ggtgtcgatg ctgccgtcgg ggctgagctc cagtttggaa
     1321 acgttgaggc cggacctcag tccaaaattc agcttggcct gcgccgcgcc cgcgagcaca
     1381 agcaacacag ccagaattat cagtgttttt ttcatggttt tctccctgaa cgcgtttggg
     1441 aacttcagga gttccgaaat atcggttaac aactaacggg catctgtgtt tttggtcaag
     1501 ttttttccca cttcgcgctg aaggccgccg gcctgccgag atttcgcttg acgcctgaac
     1561 gctttgccgc cctgatgaaa ccattgacgg tcaaactgag aaactaacca aagaggtggc
     1621 aaatgcagat atcggaattg ctggccctgc tgggatctaa ggctccggct tcccttgccc
     1681 tggaatggga caatgtgggc ctgctgctgg gggatggctc cagagatttt cacaaggcgc
     1741 tgataaccct ggacgttacc cccaacgcgg ttgacaaggc cctggagatc ggggcggacc
     1801 tgatcttgtc ccaccatccg ctgattttca ggcccctgaa gcgctttggc gaccccctgc
     1861 tgctcaagct tgctgaaaac aggatcgccg ttatctgtct gcacaccaat ctggatgtgg
     1921 cggcggacgg agtgaaccag gccctggcgg caaaactggg gttgcgggtt acgggcctgc
     1981 tcaacagcga acagggcggc aaatggcatc atctttccgt aaccgtcccg atcggccaca
     2041 cgaacaaggt ggcggaggcg gttttcgcgg ccgggggagg caggatcggc aactacagct
     2101 cctgttctac caggcaccgc atcaccggaa cctatgaatc cggcgagggc gccaaaccct
     2161 tcattcccgg cgatccaggc cagccccgga ccaccgtttc ggaggaagag ctggaattca
     2221 tggtggacga agcctcgctg gggccggtga tcgcggctgt gaaaagcgtc ca
//
LOCUS       DAOOWR010000268         2270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_47573_length_2270_cov_4.703386, whole genome shotgun sequence.
ACCESSION   DAOOWR010000268 DAOOWR010000000
VERSION     DAOOWR010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47573_length_2270_cov_4.703386"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PLB85_06390"
     CDS             complement(<1..363)
                     /locus_tag="PLB85_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOU72995.1"
                     /translation="MSFNKLAKPNKPLLRWEKIQLLIFDCDGVLTDGRIIYDSAGNES
                     KNFDAHDGMGFNLLRQTEIKTAVITGRNSPVLQRRCDDLKIDHVFQGIQNKLAKANEL
                     LEELGLGFDSVLFMGDDWN"
     gene            complement(470..1642)
                     /gene="metK"
                     /locus_tag="PLB85_06395"
     CDS             complement(470..1642)
                     /gene="metK"
                     /locus_tag="PLB85_06395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU72996.1"
                     /translation="MTALHANKPCDRNEYVFTSESVSEGHPDKVCDQISDAILDAWLS
                     RDPHSRVACETLATENRIVISGEVSSPTGVDVEVEPIVSRVISDIGYTNPDLGFDHNC
                     EIENYLHSQTTELETNEGAGDQGLMFGYACNQTKQLMPVPITMAHLLMENLAKARKQG
                     LIPGILPDAKSQVSMRYCGRRPVALETVVISTHHLPKNEAEFARMKELINEIVIRPTI
                     DEMESDARSTFSAEKYSVKINPRGMWENGGPAADTGLTGRKLIVDTYGGWAQHGGGSF
                     SGKDATKVDRSATYMARHIAKSVVASGLADECLLQFSFVIGESEPVSLMVDTYGSGTM
                     KDAELEKLIRASFPLTVKGIIDYLDLRRPIFLPTAAYGHFGRLDAGFSWEGTKALK"
BASE COUNT          471 a          617 c          631 g          551 t
ORIGIN      
        1 attccagtcg tcacccataa agagaacgct gtcgaagccc aaacccagtt cctccaaaag
       61 ctcattggct ttggcaagtt tgttttgaat gccctggaag acatggtcta tcttcaaatc
      121 gtcgcagcgg cgctgcagca caggggaatt tcgccccgtg atcacagccg tttttatctc
      181 cgtctgccgc agcaggttga agcccatgcc gtcgtgggcg tcgaagttct tgctttcgtt
      241 gccggcactg tcatagatga tgcgtccatc ggttagcacg ccgtcgcaat cgaagatcag
      301 cagttggatc ttttcccagc gcagcagggg tttgtttggt ttggcaagtt tgttgaaact
      361 catccggtct ccattaatat tgtcacgcgg caaagccctg cttcaaccgg ctctgggaac
      421 acaggatccc caaaacctgg gtgaattgac gcaaagcctg ccggaaaggc tacttgaggg
      481 ccttcgtccc ttcccaactg aagcccgcgt ccaaccggcc aaagtggccg taggcggccg
      541 tgggcagaaa gatgggcctg cgtaaatcca gatagtcgat gattcccttt actgtcaagg
      601 gaaaactggc gcggatgagt ttttccagtt cggcgtcctt catggtcccg ctgccatagg
      661 tatccaccat cagtgagaca ggttccgatt cgccgatcac aaagctgaac tgcagcaggc
      721 attcatcggc aaggcctgaa gcaaccacgc ttttggcgat gtgcctggcc atgtaggtag
      781 cgctgcggtc caccttggtg gcgtctttgc cggaaaaaga cccgccgccg tgctgagccc
      841 agccaccgta ggtgtccacg atcagcttgc gccccgtgag acctgtatcc gcggccggtc
      901 caccgttttc ccacatcccg cgggggttta tcttaaccga atatttctcc gcgctgaagg
      961 ttgaacgggc atcgctttcc atttcgtcga tggtggggcg gatcacgatt tcgtttatca
     1021 gttccttcat ccgggcgaat tcagcttcat tctttggcag gtgatgggtg gaaatcacca
     1081 ccgtttccag cgccacgggc ctgcgtccgc aatagcgcat gctaacctgg gatttggcat
     1141 ctggaaggat gccagggatc aagccctgtt tgcgtgcctt ggccaggttt tccatcagca
     1201 ggtgggccat tgtgatcggt accggcataa gttgtttggt ctggttgcag gcatagccga
     1261 acatcaggcc ctggtcgccc gccccttcgt tggtttccag ctctgtggtc tgggaatgca
     1321 gatagttctc gatctcacag ttatggtcaa aacccaggtc agggttggta tagccgatat
     1381 cactgatgac ccggctgacg atgggttcca cctccacatc cacaccggtg ggcgaggaaa
     1441 cttcaccgga aatgactatc ctgttttcag tggccagtgt ttcgcaggct acccgggaat
     1501 gggggtcccg gctcagccag gcgtccagaa tggcgtccga gatttggtcg caaaccttgt
     1561 ccggatgtcc ttccgagacc gattccgagg tgaaaacata ctcgttccgg tcgcagggtt
     1621 tgtttgcgtg taatgctgtc attttattct cctgtaatgt ttgtctgtcg tttctgccct
     1681 caattggccc ggacaaaaaa aatccggccg ctaaagccgg atgcaaacta cccgcacttt
     1741 agcacatttt gaagcggagc aaagggcaaa tcaccgcttg tttgaagcgt tgcagccggc
     1801 aacgcaacga acaggcatat tttctgcgcg gcaccaaatc agtcaaggga aatatccgcc
     1861 ccagccctca agctggctaa tggcctttgt tctttacccc tgccaatcaa agaaaactct
     1921 ctttgggaca gggcaggtta agccgaaggt tgtttccgcc ttccggcgtg tggcctggca
     1981 ggaaaagcga tgtagtttgg tccttaaggc cctttatagg gtttcggtcg cgcctcccgt
     2041 atgatgcccg catttgatgc gagaatctcg cgggaggcgt gggaggggga tggaagaagg
     2101 cgctcagggc ctggctggca ggatcttgtg gcggtcaggc gttttatagc tctcatacaa
     2161 atcaaactct ctttcagatc aagtgggtaa tccggttgtt cgagcccgta gggcgccata
     2221 acgatagcga gggagcgtaa gcccctcgta cagtggcaat aaccagatgg
//
LOCUS       DAOOWR010000269         2244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_48295_length_2244_cov_4.043856, whole genome shotgun sequence.
ACCESSION   DAOOWR010000269 DAOOWR010000000
VERSION     DAOOWR010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48295_length_2244_cov_4.043856"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1239
                     /locus_tag="PLB85_06400"
     CDS             160..1239
                     /locus_tag="PLB85_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOU72997.1"
                     /translation="MARNPDLSALDLSIAVSGLKTGDNPQPGVPVIRSIKNAGFRGKM
                     IGLVYDALESGIYLPELADEIYQMPYPSSGADAFLGRIDQILSRTRIDVIIPTLDAEM
                     ILYIRLEKELAKRGIHTFLPDERCFLLRDKTSLANFFPPRGIEVPETVMVSDPSQIAK
                     HCEKLGYPLMVKGKYYEAYKVWDLDEALQRFHEIAEGWGLPLLLQKVVPGDEFNVVIV
                     GDGEGGMLGMVPQKKLVITDKGKGFGGVVIQNPGLDAFAEKVISLLNWRGPCELEVIK
                     GNDNKYYLIEINPRFPAWARLAEGAGQNLPAVTVMKALGQDCEPLPPYRPGTLFIRHA
                     EDIISEIGVMGQISAQSELIRGKEQ"
     gene            1236..>2244
                     /locus_tag="PLB85_06405"
     CDS             1236..>2244
                     /locus_tag="PLB85_06405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOU72998.1"
                     /translation="MKKTYTAPHIERNYSGTMNKYGTKSVVRHQDSIDVIPVKKLVES
                     YGSPLLVLSESQIRRRYQHLTEVMKMHYPRLEMAWSYKTNYLGAVCSIYHQEGAKAEV
                     VSGMEYKMARRLGVPGKDIIFNGPGKRKEDLELAIREGARIQIDHLDELYLIEKTATE
                     LDTIPDVAIRVNMDTGVYPLWTRFGFNYENGEAFRAIQRLLSGKMMNIVGLHTHIGTF
                     MLDANAYYLAAKALLNLAQKVQDELGVVVKYIDLGGGFASQNTLHEQYTPGELSSPTF
                     DHYASAIGAAFNESHFVSENLPTLVLETGRALIDEAGYLISSVIGKKNLPAARAAFGS
                     TR"
BASE COUNT          547 a          651 c          625 g          421 t
ORIGIN      
        1 aaccggcctg gccatcctga aaatgctggc cgccggccaa accaaagacg agatcaggac
       61 agccatcctg caggattacg aggtgagcgc tgacaatttc gacagcgatt tcgaccacta
      121 caccctgatg ctggaagcct tcaacctggt ggagctctga tggcgcgcaa tccagatttg
      181 agcgccctgg acctcagcat cgccgtgagc ggcctgaaaa ccggcgacaa tccccagccg
      241 ggggtaccgg tgatccgcag catcaaaaac gccggcttcc ggggaaaaat gatcggcctg
      301 gtttatgacg ctctggaatc cggtatttat ctgccggagc tagcggacga gatctatcag
      361 atgccctatc cctcatccgg cgctgacgcc tttctgggcc gcatcgacca aatcctgtcc
      421 cgaacccgaa tcgacgtgat catacctacc ctggacgcgg aaatgatcct ctacatccgg
      481 ctggaaaaag agctcgccaa acgcggcatc cacacttttc tgcccgatga acgctgcttt
      541 ttacttcgag acaaaacttc cctggccaac ttcttcccgc cccggggcat cgaagtgcct
      601 gaaactgtga tggtgagcga ccctagccaa attgccaaac actgcgaaaa actgggctat
      661 ccgctgatgg tgaagggcaa gtattatgaa gcatacaagg tctgggacct cgacgaggcg
      721 ctgcagcgct tccacgaaat cgccgaaggc tggggactgc cgctgctgct gcagaaagtg
      781 gttccgggcg acgagttcaa cgtggtgatc gttggtgacg gagaaggcgg catgctggga
      841 atggttcccc agaaaaagct ggtgatcacg gacaaaggca aaggcttcgg cggcgtggtg
      901 atccaaaacc ccggcctgga cgccttcgcg gagaaagtga tctccctgct gaactggcgg
      961 ggcccttgcg aactggaagt tatcaaggga aacgacaaca aatactatct gatcgagata
     1021 aatccgcgct ttccagcctg ggcgcgcctg gctgaaggag ccgggcaaaa cctgcccgcg
     1081 gtgaccgtta tgaaggccct ggggcaagac tgtgagccgc ttcctcccta ccggccggga
     1141 acccttttca tccgtcacgc ggaagacatc atctccgaga tcggggtaat gggccagatt
     1201 tccgcccaaa gcgaactaat ccgaggtaaa gaacaatgaa aaaaacatac actgctcccc
     1261 acatcgagcg caactattcc gggaccatga acaaatacgg caccaagagc gtggtgcggc
     1321 atcaggacag catcgacgtc ataccggtga agaaactggt ggagagttac ggctcgccgc
     1381 tgctggttct ctccgaaagc cagattcgcc gccgctatca acacctcacc gaagtaatga
     1441 agatgcacta tcccaggctg gagatggcgt ggtcctataa aaccaactac ctgggcgcgg
     1501 tttgcagcat ctaccaccag gaaggtgcca aagccgaggt tgtttccggc atggaataca
     1561 agatggcaag gcgtttgggc gtgcctggca aggatatcat ttttaacggc cccggcaaga
     1621 ggaaagaaga tctggagctt gccatccgtg aaggcgcgcg catccagatc gaccatttgg
     1681 acgaactcta cctgatcgaa aagaccgcca cggaactgga taccattcct gacgtggcca
     1741 tcagggtgaa tatggacacg ggcgtttacc cgctctggac gcgttttggt ttcaactacg
     1801 aaaacggaga agccttccgg gctatccagc gcctgctgag cggcaagatg atgaacatcg
     1861 tgggcctgca cactcacatc ggcaccttca tgctggacgc gaacgcctat tacctggcgg
     1921 ccaaagcatt gctgaacctg gcacagaagg tgcaggacga actgggtgtg gtggtgaaat
     1981 acatcgacct cggcggcggc tttgcctcgc aaaacactct tcacgagcag tacacaccgg
     2041 gagagctttc ctcccccact ttcgaccatt acgcttccgc tatcggcgcc gccttcaacg
     2101 aatcgcactt tgtgagcgaa aacctgccca ccctggttct ggagaccgga cgcgccctaa
     2161 tcgacgaagc gggctatctg atcagctccg tgatcggcaa aaagaacctg ccggcggcaa
     2221 gggcggcatt cgggtcgacc cgat
//
LOCUS       DAOOWR010000270         2230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_48697_length_2230_cov_4.246897, whole genome shotgun sequence.
ACCESSION   DAOOWR010000270 DAOOWR010000000
VERSION     DAOOWR010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48697_length_2230_cov_4.246897"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLB85_06410"
     CDS             <1..302
                     /locus_tag="PLB85_06410"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOU72999.1"
                     /translation="LYESGLKLCWFRSLTGINCPACGLSRAFLSLFRGRFVQAFLYNP
                     FMLLFSLGMFLQLFSTTVFKRRIALEASASERNLLLVAFLVLFLLNWLYLILFLP"
     gene            569..1126
                     /locus_tag="PLB85_06415"
     CDS             569..1126
                     /locus_tag="PLB85_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73000.1"
                     /translation="MKTNISQFKIRLVSRWSLVLLLLVCVGAGFARVGLSSGEINVSG
                     SDHALQTTFDTQSAKTAPDYPDSDAETSGTRTPAWLQIILTVGIALLIYYVILAIVKS
                     REKSREEVIHVKTRESDTGYTMERPGSQVSVPERQPVEYRVPPQKPSESARKPRTAKR
                     DSSWPFIIAIIVLVNIIRQCVQNFD"
     gene            1802..>2230
                     /locus_tag="PLB85_06420"
     CDS             1802..>2230
                     /locus_tag="PLB85_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HOU73001.1"
                     /translation="MRKLRCAILLILLLMYLSLIYAVNDMTVIAAFQGEHHNSGYGYS
                     MVSLDFNHDGFDDLAVCSPSYDYVYPGTIHRGHGKLYIYFGTNGFNSNTYPSLTYEGT
                     YDSTMVGRQIKGIFRVGDVSGDGFDDLCVYVEDHISWNESY"
BASE COUNT          532 a          556 c          564 g          578 t
ORIGIN      
        1 tgctgtatga atccggtctg aagctatgct ggttccgctc cctgacaggc atcaactgcc
       61 cagcctgcgg tctttcccga gctttcctgt cccttttccg aggccgcttt gtccaggcct
      121 ttctttacaa tcccttcatg ctcctattca gcctcggaat gttcctgcaa ctgttctcca
      181 ccactgtgtt caagcgccgt atcgcccttg aggccagcgc tagcgaacgc aacttactct
      241 tggtcgcctt cctcgtcctc tttttgctca actggcttta tctgatcctg tttttgccct
      301 gacccgtcct ttgccccatc ccgtccgcct ccttttcgcc tcccgctgag ttcccgcctt
      361 tcaaacggga agtccgcggg aagtgaatgg gatgtgaaag ggatagggca aagggcctgt
      421 agccattcag agcaggaatg atggtgggaa tcgctgaata tgatgcctat aaaggagtta
      481 accggtctgg atgctgcggg gaggatttag cttgacagca ccggggtggg ccggaaactg
      541 gtggtaaact gtaaccacga ggatgagaat gaaaacaaat atttcccagt tcaagattcg
      601 gctcgtttca cgctggagcc tggtattgtt gctgcttgta tgcgttggtg caggttttgc
      661 ccgggtgggg ttaagttctg gcgaaataaa cgtttctggc tccgatcatg cgctgcaaac
      721 cactttcgac acccaatcgg cgaaaaccgc gcctgattat ccggattcag atgccgaaac
      781 aagcggaacg cggactcctg cctggttgca gatcattctc acagtgggca tcgcgctatt
      841 gatttactat gttatcctgg cgatagttaa gagcagagag aaatccagag aggaggtcat
      901 tcacgtaaaa accagggaaa gtgatactgg ctacacgatg gagcgacctg gcagccaggt
      961 ttctgtgcca gaaaggcagc cggttgaata ccgcgttccg cctcaaaagc cgtccgaaag
     1021 cgcgagaaag ccgagaacgg caaaaaggga ctcctcctgg ccttttatta tcgccatcat
     1081 tgtgctggtc aacatcatcc gccagtgcgt gcaaaacttc gattgagcgt gtcggacacc
     1141 tggtccggtt cagttcagcc tctagccgtc agcctcgcct ctgctgattc cgcccgcgaa
     1201 ggctgcgcaa ggtaatagtc tggttcaagt tcagctatgg tggataaact agatttccgc
     1261 cctacattcc gggcttgacc cggaatccag aaaaccccgc cacctcatcc cctggcgtcc
     1321 agcgaacgca gagagcatgg acggcaggaa atgttgctgt cgtcgatgct ggtgtagcct
     1381 ggagtccagc gaacccgtga gcttggacta caggagttgc tgctgtcgcc tctgcgaggc
     1441 tccgttggag tttctgtccc ccatcaggct cagaaactac ctgggttacc tcccattttg
     1501 acaaagggca aaaacagacc ttgacaagaa taacgcaagc gtaaaaatat gcgtgtaggc
     1561 cttggaatga ataaaggata ccattgagcc gggattgctt cctaccaaag aacgcagaag
     1621 aagataaatg tcgtgtgttg aaaaatacat tcattagggg aaaagatgat agagacagat
     1681 ttttctgctc atttgtagtt catgaaccac aagaaggatg cgttttcttg gcctgtccct
     1741 gtctggcaag atggcgcgca attctgtcat gtttatttac ccataacttg ctggaggaac
     1801 gttgagaaag ttacgatgtg caatcctcct gatcctgctg ctaatgtacc tgagcctcat
     1861 ttatgcagtc aacgatatga ccgtcatcgc tgcctttcag ggtgagcacc ataactcagg
     1921 ttacggatac tcgatggttt cccttgattt caatcatgac ggttttgatg atctggcagt
     1981 atgttctccc agttacgatt atgtatatcc aggcacaata catagaggac atggaaagct
     2041 ctatatttat tttggaacca atggattcaa ctcaaacact tatccatctt taacttatga
     2101 aggcacttac gatagcacga tggttggcag acaaattaaa ggtatcttca gggttggtga
     2161 tgtcagtgga gatggctttg atgatctctg tgtgtacgta gaagaccaca tatcctggaa
     2221 cgagagttat
//
LOCUS       DAOOWR010000271         2227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_48778_length_2227_cov_4.178637, whole genome shotgun sequence.
ACCESSION   DAOOWR010000271 DAOOWR010000000
VERSION     DAOOWR010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48778_length_2227_cov_4.178637"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="ispG"
                     /locus_tag="PLB85_06425"
                     /gene_synonym="gcpE"
     CDS             <1..1047
                     /gene="ispG"
                     /locus_tag="PLB85_06425"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOU73002.1"
                     /translation="PTRQIHLGNVQIGGGAPVSIQSMLSVPTRDLEAALAQTASLEQA
                     GCQIIRFAVTGAEDIRAIPVLAENARVPLVADIHFDHKLALQALEAGISGLRINPGNI
                     GSRAKVEAVVKAASERGVPIRIGVNSGSLPKEMVAKYGVSAEAMVEAALEHVRILEDL
                     NFGAIKISVKASHLPLMLESYRALSARVDYPLHLGITESGTLLAGSVKSAMALGILLS
                     EGIGDTLRVSLTADPIQEVAVAKQILRNLELRQGLNIISCPTCGRTRVDLFSLASEVE
                     SALQAYSDLPLTIAVMGCAVNGPGEAREADFGIAGGDGEGLIFARGEILKKVPERQLV
                     AELIHLVEEWVNNV"
     gene            1040..1588
                     /locus_tag="PLB85_06430"
     CDS             1040..1588
                     /locus_tag="PLB85_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU73003.1"
                     /translation="MSKSLGSIFLTFLKIGAFTIGGAYAMIPLIRREVVEKHGWISDD
                     DFLDGLAAAQSCPGPIAINISVYVGLHVRGRLGMAAAVLGSVLPSLVIIMLIAELFVH
                     YAEQALVRKAFHALKPALVALIAVPLAEMTKTANLNLTNFWVPIVALVLVGFLGVSPI
                     WLILATIAFAVAQGLWRKEKPE"
     gene            1585..2103
                     /locus_tag="PLB85_06435"
     CDS             1585..2103
                     /locus_tag="PLB85_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOU73004.1"
                     /translation="MTWLTLFWTFVKIGLFSFGGGYAVLAMIQQEVVVRNAWLTQSEF
                     TDVVAISQMTPGPIAINSATFIGYRQGGVLGSLVCTFGVILPSLVLMLVITLTYLKLR
                     EQPWFKNIFQKLRWLTLGLIGAAMVLIAKGAFTDWFTVLVFVISLAIYWKFKTNPIYL
                     MLGAAVFGMVFG"
BASE COUNT          427 a          660 c          622 g          518 t
ORIGIN      
        1 cctacccggc agatacactt gggcaatgtc cagatcggcg ggggcgcgcc tgtttctatc
       61 caaagcatgc tcagcgtgcc aacccgcgat ttggaagcgg ccctggccca gacagcctct
      121 ttggaacaag ccggctgcca gatcatccgc ttcgctgtaa ccggcgcaga ggatatccgg
      181 gcgataccag ttttggcgga aaacgccagg gttcccctgg tggcagacat ccatttcgac
      241 cacaaactgg ccttgcaagc cctggaagcc ggaatctccg gacttcgcat caatccaggc
      301 aacatcggct cccgcgccaa ggttgaggcc gtggtgaagg ctgcttccga aaggggggtg
      361 cccatccgca tcggcgtgaa tagcggttcc ctgcccaaag agatggtggc taaatacgga
      421 gtgagtgccg aagccatggt ggaagcggct ttggagcacg tgcgcattct cgaggacctg
      481 aactttggcg ccatcaagat ttccgtgaag gcctcgcacc tgcctttgat gctggaaagt
      541 taccgcgccc tcagcgccag ggtggattat cctttgcatc tgggcatcac ggaatccggc
      601 accctgctgg ctggcagcgt aaaatccgcc atggctctgg gaatcctgct ttcagaaggc
      661 atcggcgaca ccctgcgggt ttcactaacc gcggatccca tccaggaagt ggctgtggcc
      721 aagcaaatcc tgcgcaacct tgaactgcgc caggggctta acatcatttc ctgtcccacc
      781 tgcggacgca cccgggtgga ccttttcagc ctggcttccg aggtggaaag cgcccttcaa
      841 gcctattccg atttgccgct caccatcgcc gtgatgggct gtgccgtgaa tggccccgga
      901 gaagcgcggg aagcggattt cggcattgca ggcggggacg gggagggact catctttgcc
      961 cgcggcgaaa tcctgaagaa agtgccggaa cggcagcttg tggctgagct aatacatctc
     1021 gtggaagagt gggtgaacaa tgtctaaaag tcttggctcc atattcttaa cctttctcaa
     1081 aatcggtgct ttcaccatcg gtggcgccta tgccatgatt cccctcatcc gccgtgaagt
     1141 ggtggaaaaa catggctgga tctctgacga tgattttctg gacggcctgg ctgcggcaca
     1201 gtcctgcccc gggccaatag ccatcaacat cagcgtttac gttggtctgc atgttcgagg
     1261 ccgcctgggc atggcggcag cggtgctggg ctcggtgctg ccttccctgg tcatcatcat
     1321 gctgatcgct gaactctttg tccactacgc ggaacaagcc ctggtgcgca aagcttttca
     1381 cgcgctcaaa ccagcactgg tggccctcat cgcggtgccc ctggcggaaa tgaccaagac
     1441 cgccaatctc aatcttacca atttctgggt gccgattgtc gctctggtgc tggtgggctt
     1501 tcttggcgtc agtcccatct ggcttatttt ggccacgatc gcctttgccg tggcgcaggg
     1561 tttgtggcgg aaggagaagc cggaatgact tggctgactt tgttctggac cttcgtaaaa
     1621 atcgggcttt tcagctttgg tggcggctac gcggtcctgg ccatgatcca gcaggaagtg
     1681 gtggtccgca acgcctggct cactcaaagc gaattcacgg atgtggtggc gatttcgcaa
     1741 atgacccccg gccccatcgc catcaactcc gccaccttca tcggctaccg gcagggaggg
     1801 gttttgggct ccctggtctg caccttcggc gtcatcctgc cctcgcttgt cctgatgctg
     1861 gtcataaccc tcacctatct caagctccgc gagcagccct ggttcaaaaa catttttcag
     1921 aaactgcgct ggctgaccct cggcctcatc ggcgctgcca tggtgctcat cgccaaaggc
     1981 gctttcactg attggttcac agtgctcgtg tttgtgatca gcctggcgat ctactggaag
     2041 tttaaaacca accccattta tctgatgctg ggcgctgctg tcttcgggat ggtcttcggg
     2101 tgaacaatcc attgctgtgt atgcggttaa ccactattaa aaaatgccat tatttgcttg
     2161 attcgccctt tgccccatcc cactcacatc ccgttcactt cccgctgagt tcccgccttt
     2221 caaacgg
//
LOCUS       DAOOWR010000272         2220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_49037_length_2220_cov_2.730716, whole genome shotgun sequence.
ACCESSION   DAOOWR010000272 DAOOWR010000000
VERSION     DAOOWR010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49037_length_2220_cov_2.730716"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLB85_06440"
     CDS             complement(<1..361)
                     /locus_tag="PLB85_06440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU73005.1"
                     /translation="MDDLIVFSVFEITRHLKQVVETQIEPLYVRGEISNFTRHSSGHI
                     YFNLKDPNATLRCTFFRNANFNLSFAPEEGMEVVCYGRLTVFEKGGYYNLNVQSMELS
                     GKGDLARQFELLKAKLQE"
     gene            complement(411..>2220)
                     /locus_tag="PLB85_06445"
     CDS             complement(411..>2220)
                     /locus_tag="PLB85_06445"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU73006.1"
                     /translation="WAGLNQMLGLLEQNGIVYSGAGIDSYQAYTPAFINRNGLNLAFL
                     ASSDRTGQYNNAQPYLQAGYNKPGFAYMTPYYITQQLNAVAEVADLRIVEMHGGSEYS
                     LTPGSGYDKSDNPFLGDDQDEDYSPRTDVPHLWDIAIRHWAVDSGADLVIVHHPHIIQ
                     GFEVYQGKVIAHSLGNFAFDLDYPETMPSLIFSADADRSGFSNFLIQPVFIDGYIPKP
                     ATGQLGIYILDYLAMRSREFNTILVVDNNRMSASVLLDSSQAPSSSHPYTYNLLLEPV
                     TGQGNVSKPLKLPRYGSISSVNAIEPVSNADYRLGRETIWYGNFEDEGSTLWDLQSFS
                     YNDVFDGERSALISAPPGQTRMAGIKKLCKWYDNSKKFILHGWIKTVNAGNANIQVRY
                     YNSRQGYQIGSEYITADISGNTEWTWYYKELTLPPNAWYFDIRLTCTGASSGQTQALF
                     DNVGLIEWTSWTAWEANNSVINPNDYYWLQIQTPENPKSVNVHLTEQRYNPLPDRSKA
                     VPPVQMQLSVHPNPFRESAKLNFELSGKGPVSLSVYNIRGQKVATLAEGELPAGKHQL
                     AWNGKDRQGRQAASGLYFIRLEQEGRSVVRKAVLLR"
BASE COUNT          470 a          560 c          647 g          543 t
ORIGIN      
        1 cctcctgcag cttggctttc aaaagctcga actgcctggc gaggtcgcct tttccggaaa
       61 gctccatgct ctggacgttt agattgtaat agccgccttt ttcgaacacc gtcagcctgc
      121 catagcaaac cacctccatc ccttcttccg gagcgaaact caggttgaag ttggcgttgc
      181 ggaaaaaggt gcagcgtagt gtcgcgttgg ggtctttgag attgaaatag atgtgcccgg
      241 agctgtgtcg ggtgaaattg ctgatctcgc cgcgcacgta gagcggttcg atctgggttt
      301 ccaccacctg cttcagatgc cgggttatct caaaaacgct gaatacaatc aaatcatcca
      361 tgataagcca tcaaatccgg gcatggatat ttgtcaatga ccggctgctc ctaacgcaga
      421 agcaccgcct ttcggactac cgatcttccc tcctgctcca aacgaatgaa atacaaccca
      481 gaagcagcct ggcgaccttg ccggtctttt ccgttccagg ccagctggtg cttgccggcg
      541 ggaagttcgc cttccgccag ggtggccact ttctggccgc gtatgttata cactgacaaa
      601 gaaacagggc cttttccgct aagctcgaag tttagtttgg cgctttcccg gaagggattg
      661 ggatggacgg aaagctgcat ttgcacggga ggaacagctt tggacctatc cggaagcggg
      721 ttgtagcgtt gttcggttag atgaacgttc acggatttgg ggttttcggg ggtctgaatt
      781 tgcaaccagt aatagtcatt ggggttgata acgctgttgt tggcttccca agcggtccaa
      841 gacgtccatt cgatcaaacc gacgttatca aaaagcgcct gtgtctggcc ggaagaagct
      901 cctgtgcagg tgaggcggat atcgaaatac caagcgttgg gagggagggt gagttccttg
      961 taataccagg tccattcagt gtttccggaa atgtcggcgg tgatgtattc gctgccgatt
     1021 tgataaccct ggcggctgtt gtaataacgc acctgaatgt tggcattgcc tgcgttgacc
     1081 gtcttgatcc agccgtggag gatgaacttt ttactgttgt cgtaccactt gcagagtttt
     1141 tttatgccgg ccattctggt ctgaccaggt ggagccgaga taagggcgct gcgctctccg
     1201 tcgaaaacat cgttgtagga aaagctttgc aggtcccaga gagtggagcc ctcatcctcg
     1261 aaattgccat accagatggt ttccctgccc agcctgtaat ccgcgttact gacaggttcg
     1321 atggcgttca cggagctgat gctgccgtag cggggaagtt tcagcggttt tgaaacgttg
     1381 ccctggcctg tgaccggctc cagcagcagg ttgtaggtat agggatggct gctcgaaggg
     1441 gcttggctgc tgtctaacaa cacgctggcc gacatgcggt tgttgtccac caccaggatt
     1501 gtgttaaact cgcggctgcg catggccaga tagtccagaa tgtaaatccc taattggcct
     1561 gttgcgggtt tggggatgta tccgtcgatg aaaacgggct ggatcagaaa gttgctgaag
     1621 ccgctgcgat cggcatcggc agagaaaatc agcgaaggca tagtctccgg ataatccagg
     1681 tcaaaagcga agttgcccag cgaatgcgcg atcactttgc cctggtaaac ctcaaaaccc
     1741 tggatgatat ggggatggtg cacgataacg aggtccgcgc cggaatccac tgcccagtgc
     1801 cggatggcga tatcccaaag gtgcgggaca tccgttcggg gcgagtaatc ttcatcctgg
     1861 tcatcaccga ggaaggggtt gtcgctcttg tcgtaaccgg agccgggggt cagcgaatac
     1921 tcgcttccac cgtgcatttc cactattctc aggtcagcca cttccgccac tgcgttcagt
     1981 tgctgcgtaa tgtaatacgg cgtcatgtag gcgaagccgg gtttgttgta gcctgcctgc
     2041 agatagggct gggcgttgtt atactgaccg gtgcggtcgc tgctggccag gaaagcgagg
     2101 ttgagcccgt tgcggttgat gaaggcgggg gtgtatgcct ggtaggaatc aattcccgcg
     2161 ccactgtaca caatgccgtt ctgctccagc aatccgagca tctggttgag gccagcccag
//
LOCUS       DAOOWR010000273         2213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_49212_length_2213_cov_3.200649, whole genome shotgun sequence.
ACCESSION   DAOOWR010000273 DAOOWR010000000
VERSION     DAOOWR010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49212_length_2213_cov_3.200649"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(635..976)
                     /locus_tag="PLB85_06450"
     CDS             complement(635..976)
                     /locus_tag="PLB85_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOU73007.1"
                     /translation="MTDKEKNGCKCGEDCHEHDGLCDCENEECDCNTITIEMDDGSSK
                     DFIVLDVLTHEGKQYIALAEVDSMEYDIMSWEVDGENVELSVIEDDDEFNAVAAKFEE
                     LFKSDNEEPED"
     gene            complement(1049..>2213)
                     /gene="priA"
                     /locus_tag="PLB85_06455"
     CDS             complement(1049..>2213)
                     /gene="priA"
                     /locus_tag="PLB85_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU73008.1"
                     /translation="KLEGAIIVLGSATPSLESWHNQNIGKYTLHTLKSRPLDIKLPEV
                     RILSLQEDYQQQLLSDELIAAIGARLEKKEQVILFQNRRGFSSYMQCLKCGELIKCAN
                     CDISMYYHRDREEMNCHYCGNAYPSPRKCPSCGSYTFSYGSPGTQKVEQLLRIVFPQA
                     RVLRLDSDSARRQDSYKTMYRRMKDRDVDILLGTQMISKGLDFPAVTLVGIINADISL
                     NVPDFRSAERTFQLCTQVAGRSGRADKRGEVIIQTYNPTHYAIVHAGNQDYQAFAEEE
                     LEHRRRLNYPPFYRLARILFQCSDGNLLEQEMAFLREKALLAAASFGANDLYLLGPAP
                     APFARINKLNRWHLILKARTPSILKQALKRISELYTPPAAIHSHPDVDPLNLM"
BASE COUNT          428 a          596 c          652 g          537 t
ORIGIN      
        1 gacccgatct gcgtccgggt ctgggtctgc gtcggcgtct ccggcgtgcc ctgcggcagc
       61 ccctcgaacg gcgaggcctg gccgaacgcc ccggccgtcg gcaggagcag tgcgaagagg
      121 gtgagcagga gcgcgaagcg ggctgccggg cggatgacac gggacacggg catgacgcgc
      181 gggcacgata cccggcggag cggcgcgcca cccgcgcggc tatcgtgccc cggatgccgc
      241 ctccggccag ggatatctcc acataggatt ttccttccag gcgcagggcg aattcgtcct
      301 cacgggtgtg cacgaaaact ggatgggtgt ggcagtctac gaaaccgggc aaaaccagtt
      361 tatcatccgc ggaaaccact tccggggcgg aatacgcggc catgatctca gccgttgtgc
      421 tcacagcaag gatccgtccg tctttaacgg ccagagctcc gccagctatg atgccggggt
      481 cgctcatcgc agatacggtg cggggttcag cgcctcccct cagcgttaca agttgcctgc
      541 aatcctttag tattaggtca gcctgcactt ttcaacctcc cgttcatgcg cctgacgcgt
      601 ttatttgtat attgttccct ttgcccgttt cagatcagtc ttcgggctct tcgttatcgc
      661 ttttgaacaa ttcttcgaat ttggctgcca cggcattgaa ctcatcatcg tcttcgatca
      721 cgctcagttc aacgttttcg ccatccactt cccagctcat gatgtcgtat tccatggaat
      781 cgacctcagc caaagctatg tactgcttgc cttcgtgggt gagcacatcc agcacgatga
      841 aatccttgct gctgccgtca tccatttcga tggtgatggt gttgcagtcg cattcttcgt
      901 tttcgcagtc gcaaagtcca tcgtgttcgt ggcaatcttc gccgcatttg cagccgttct
      961 tttctttatc tgtcatgggg aactcctttg tttttcacac tttttgccgc ttgagtattt
     1021 tggcaaggga aaaaattggc ggatgttatc acatcaggtt tagcgggtcc acatctggat
     1081 gggaatgaat ggccgcgggc ggggtgtaga gctctgagat ccgcttcagg gcttgcttca
     1141 ggatagaagg ggtgcgggct ttgaggatca gatgccagcg gttgagcttg ttaattctgg
     1201 caaatggtgc aggtgccgga cctaatagat agagatcatt cgccccgaaa gaggctgctg
     1261 ccagcagggc tttttcccgc aggaaagcca tttcctgctc gagcagattc ccgtccgaac
     1321 actggaacag gattctggcc agcctgtaga agggcgggta gttcaagcgc cggcgatgtt
     1381 ccagctcttc ttccgcgaaa gcctggtaat cctgatttcc agcgtgcacg atggcgtaat
     1441 gggtggggtt gtatgtctgg ataatcacct cgccacgctt gtccgcccgg ccggagcgtc
     1501 ccgcaacctg ggtgcagagt tggaaagtgc gctccgcgga acggaaatcc ggaacgttta
     1561 ggctgatgtc agcgttgatg attcccacca gggtcacagc cggaaaatcc aagcccttgg
     1621 aaatcatctg cgtgcccaaa aggatgtcca cgtcgcggtc tttcatgcgg cggtacatgg
     1681 ttttatagct gtcctgacgg cgcgcagaat ctgaatccag gcgcaacacc ctagcctggg
     1741 gaaagacaat tcgcagaagc tgttcaactt tttgggtgcc gggcgagcca taggaaaaag
     1801 tgtagctgcc gcagctgggg cacttgcgtg ggctgggata agcatttccg caatagtggc
     1861 agttcatttc ctcacggtcg cggtggtaat acatgctgat gtcgcagttg gcgcatttga
     1921 tcagctcgcc gcatttcagg cactgcatat aagaggagaa gccgcgtcgg ttctggaaca
     1981 ggatcacctg ttcctttttc tcaaggcggg cgccaatggc ggcgataagt tcgtcggaaa
     2041 gcaattgttg ctggtagtct tcctgcagcg aaaggatgcg aacctcgggc agcttgatgt
     2101 ccagcggtct gcttttgagg gtgtgaagtg tgtatttgcc gatgttctga ttgtgccagg
     2161 attccagcga aggggtggcg ctgcccagca cgattattgc cccttccagc ttg
//
LOCUS       DAOOWR010000274         2197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_49724_length_2197_cov_3.381419, whole genome shotgun sequence.
ACCESSION   DAOOWR010000274 DAOOWR010000000
VERSION     DAOOWR010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49724_length_2197_cov_3.381419"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PLB85_06460"
     CDS             complement(<1..1236)
                     /locus_tag="PLB85_06460"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU73009.1"
                     /translation="MTNLAPLFAILLALALLLAGCGANQGKLAGRINGQPVTAESFND
                     AYRGHYANFQWLNNRAPSREERELIKQQTWTDAARGVILRQWFAKYKISATPSEVVDT
                     LSKNIPSYILSSPLFMTDGVFDPKIYHQSLLYDSPENLAILRQDFLDWKVPIMKLKQE
                     LIVRELLDKKERQLITGILQSSADVEFTLLELAGIDPYVSQDEVRAHYQKNLADYRLE
                     PFVSLSCATMEVLPSRTDIHHANLYADSLYQELSAGTPVSALLEGNHPLAASLVFKNS
                     GFLSVASLDPIVYKQLSELEEGAWGRPLQTASGTTLHQLEKRTKSMCSFNSLWLPYRP
                     SASRIEALRPEAELAVKLARLKGHATACEEMDLPRYKVHKANPDSLWYPDARIVEAIR
                     AQLPGQPSGHVFDALYSDST"
     gene            complement(1233..>2197)
                     /locus_tag="PLB85_06465"
     CDS             complement(1233..>2197)
                     /locus_tag="PLB85_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU73010.1"
                     /translation="TLALTDVLSSLAWLAWQNNYCCPVFTEQRTLTIEAGRHPVIEQL
                     IEDQEFIPNDTSLDYPDTTIAIITGPNMAGKSTYLRQVGLLVIMAQIGSFVPAKHLSM
                     PIFDRVFTRVGASDNLAQGQSTFLVEMIETANILHTATENSLILLDEIGRGTSTFDGL
                     SLAWSIIEYIQKYKHSLTLFATHYHELTELENIYPDIRNFNVVVKEYNDQMIFLRKIE
                     RGGADQSYGIQVARLAGIPDRVIRRAREILRNLEDHEISPQGLTASIRRKLNRTSPQI
                     ELFEVLADKVSEIDPIVDEIKDLDLDSLTPIEAWQKLKDLQDRL"
BASE COUNT          417 a          512 c          765 g          503 t
ORIGIN      
        1 ggtggaatct gaatagaggg cgtcgaaaac atgaccggag ggctgaccgg gcaactgggc
       61 gcggatggct tccacgattc gggcatccgg ataccagagg ctgtcgggat tggccttgtg
      121 cactttatag cggggcaggt ccatttcctc gcaagcggtg gcgtggcctt tcaggcgggc
      181 aagcttaacc gcgagttcgg cttcgggacg cagggcttcg atcctggaag ccgagggacg
      241 gtagggcagc cagagggaat tgaagctgca catgctcttg gtgcgttttt ccaactggtg
      301 gagcgtggtg ccgctggcag tttgcagagg ccgtccccag gcgccttcct ccagttcgct
      361 gagttgcttg taaacaatgg gatcgaggga ggcaacgctg agaaatccgg agtttttgaa
      421 aaccaggctg gcggcgaggg gatggttgcc ctccaacagg gcggagactg gcgttccggc
      481 gctgagctcc tgatagaggg aatcggcgta gaggttggcg tggtggatgt cagtgcggga
      541 aggcaggacc tccatggtgg cgcaggacag gctgacaaag ggttccaggc ggtaatccgc
      601 caggtttttc tgataatggg cgcgcacttc atcctgcgaa acatagggat cgatgccggc
      661 cagttccagc agggtgaatt ccacgtccgc gctgctctgg aggatgccgg tgatgagctg
      721 gcgttctttt ttgtccaaaa gttcccggac gatgagttct tgcttaagct tcatgatggg
      781 caccttccaa tccaggaaat cctggcgcag tatggcgagg ttttccggcg agtcgtaaag
      841 cagcgactgg tggtatatct tggggtcgaa gacgccatcg gtcatgaaga ggggggagct
      901 gaggatgtag gaggggatgt ttttggagag ggtgtccacc acttcggagg gggtggcgct
      961 gattttgtat ttggcgaacc attgcctgag gatcacgccc ctggcggcgt ctgtccaggt
     1021 ttgctgcttg atcagttccc tttcttcgcg ggagggggcg cggttgttga gccactgaaa
     1081 gttggcgtaa tggccgcggt aggcatcgtt gaaagattca gcggtgacgg gctggccgtt
     1141 gatgcgtccg gccagtttgc cctgattggc accgcagccg gccagcagca aagccagcgc
     1201 cagcagtatg gcgaagaggg gggcgagatt ggtcataatc tgtcctgtag gtcttttagt
     1261 ttttgccagg cctcgatggg ggtgaggctg tcgaggtcga ggtccttgat ctcatccacg
     1321 atggggtcga tttccgagac tttatccgcc agcacctcaa agagctctat ctgggggctg
     1381 gtgcggttca gtttgcggcg gatggaggcg gtgaggccct ggggcgatat ttcgtggtcc
     1441 tcaaggttgc gcaggatttc ccgggcacgg cggataacgc ggtccgggat gccagccagc
     1501 cgggccacct ggatgccgta actctggtcg gcgccgccac gctcaatctt gcgcagaaag
     1561 atcatctggt cattgtattc cttcaccacc acattgaaat tgcggatgtc cggatagatg
     1621 ttttccagtt ccgtgagctc gtgatagtgg gtggcgaaga gggtgaggct gtgtttgtat
     1681 ttctggatgt attcgatgat ggaccaagcg aggctgaggc cgtcgaaagt ggaggttccg
     1741 cggccgatct cgtccagcag gatgagtgag ttttcggttg cggtgtggag gatgtttgcc
     1801 gtttctatca tctccaccaa gaaggtgctc tggccctggg cgaggttgtc ggaagcgccc
     1861 acgcgggtga aaacgcggtc gaaaatgggc atggaaagat gtttggcagg cacgaaactg
     1921 ccgatctggg ccataatgac tagaagtccc acctgacgca gataggttga tttccccgcc
     1981 atattcggtc cggtgataat ggcgatggtg gtatctgggt aatccaggga agtgtcgttg
     2041 ggaatgaatt cctggtcctc gatgagctgc tcgataactg gatggcggcc ggcttcaatg
     2101 gtgagggtgc gctgctcggt gaaaacggga cagcagtagt tgttttgcca agccagccag
     2161 gccaggctgc tcaacacgtc tgtgagggcg agggtgg
//
LOCUS       DAOOWR010000275         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_49881_length_2192_cov_3.618156, whole genome shotgun sequence.
ACCESSION   DAOOWR010000275 DAOOWR010000000
VERSION     DAOOWR010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49881_length_2192_cov_3.618156"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2192)
                     /locus_tag="PLB85_06470"
     CDS             complement(<1..>2192)
                     /locus_tag="PLB85_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOU73011.1"
                     /translation="YGVLAHQFGHILGLPSLYDNDSSNGASQGIGNWGLMGTGIWNAS
                     GYVPAQVSAWCRTFLGWETPITVTETTENLSISHFLNHMPVNDRVYKIPISDREYFLA
                     ENRQQNPDGSLNPYNNLPSYSFVLLPPGEQDYYENFPELPFFNFMENRYKGSEWDFML
                     PGLGGPVPAGMPVPVDGSGLLIWHIDENVIAQNFTANFDYNTINAYSQHKGIDLEEAD
                     GIQHLDVSAADQYKYGSPYDSFRAGNNDYFGKQLHNGILSLPTAESYYGGVPVEIHDI
                     SASGNNMTFSVRFGWRLSTGFSGENNINACAVDFDGDGNTELIYPQPDGQIHIWKDEL
                     PLPGYPICGQPMEHTYVWTGSEFYFPMQEENQARLYRLDSAGSEYIHTFEDMSWATHP
                     LSTGESLYMALNSNLPGQRGTLFSHDPETSEVQTLREFEYPILANMTWFRGRLYIPSR
                     QEGMFCVWKLDPNNPMFAECLLLDVPLDSTLVAVFVAPLRPQNPSSQGEVIVQCVNSI
                     YVFDSDKNILPGFPYVHDMRSTAPLSIEDWDKNGTLDLIISSDRGVAVIDYSGSRMSP
                     ASLNLAASDSLAFSSGVLAVDIDHDGKKELLGSFGFNQLNCWDNSFRPKPGYPVSFGQ
                     RNRNLPLIAAGSDGLVYAWLASDNGFIYRQALPEAVMADLQSGWTAEFGSLLRQASRD
                     SNNFPNQYQTTELFVPGEFYIYPNPLKSIHEQKLTLNVMTSRDTALEV"
BASE COUNT          474 a          524 c          682 g          512 t
ORIGIN      
        1 ttcacttcca gggcagtgtc gcggctggtc atgacattca gggttaattt ttgttcgtgg
       61 atggacttga gggggttggg atagatgtag aattctccgg gcacgaagag ttctgtggtt
      121 tggtactggt ttgggaaatt attgctgtcg cggctggcct ggcggagcag agagccgaat
      181 tcagcggtcc atccggattg gaggtctgcc atgacagctt caggcagggc ttggcggtag
      241 atgaagccgt tatcggaagc cagccaggca tatacaaggc cgtctgaacc agcggcgatg
      301 aggggcaggt tacggttgcg ttgtccgaag gaaactgggt aaccgggttt agggcgaaaa
      361 ctgttgtccc agcaatttag ctggttgaag ccaaaagagc caaggagttc cttttttccg
      421 tcgtgatcaa tgtccacggc cagaactcca gaactgaagg ccagggaatc cgaagccgcc
      481 aggttcagtg aagccggact catccgcgaa ccggagtagt cgatgacggc gacgccccgg
      541 tctgaagaaa tgatgaggtc cagagtgccg ttcttatccc agtcctctat gctcagcggc
      601 gcggtggagc gcatgtcatg aacataggga aatccgggca ggatgttttt gtcggagtca
      661 aacacataga tggaattgac gcactgaact atcacctcgc cctggctgga aggattctgc
      721 ggccgtaggg gagccacgaa aaccgccacc agggtggaat ccagaggcac gtccagaaga
      781 agacattccg caaacatggg gttattcggg tccagtttcc aaacgcagaa cattccttcc
      841 tgacgggagg ggatgtaaag ccggccgcgg aaccaggtca tgttggctag gattggatac
      901 tcgaattcgc gcagggtctg aacttcgctt gtctccgggt cgtggctgaa cagcgtacct
      961 cgctgtccag ggaggttgct gttcaaggcc atatacaacg attcgcccgt gctgagggga
     1021 tgggtggccc aggacatgtc ctcaaatgtg tgaatatatt cgctgccagc agagtccagg
     1081 cggtagagcc gggcttgatt ttcctcctgc atgggaaaat agaattcgct gccggtccag
     1141 acgtaagtgt gttccattgg ctgaccgcag atcggatatc ctggcagggg cagttcgtct
     1201 ttccagatgt ggatctggcc gtcaggttgg ggatagatca gttctgtgtt gccgtcgccg
     1261 tcgaaatcca ctgcgcaggc gttgatgttg ttctctccgc tgaacccggt ggaaagcctc
     1321 cagccaaacc ttaccgagaa ggtcatatta ttgccgctgg cgctgatgtc atggatttcc
     1381 acagggacgc cgccatagta gctttcagcg gtgggcaggg agagaatccc gttgtgtaat
     1441 tgcttcccga aataatcgtt gttgccggcg cggaagctgt cataaggact gccgtatttg
     1501 tattggtcgg ctgccgatac atccagatgc tggatgccgt cggcttcctc aaggtcgatg
     1561 cctttgtgtt gggagtaggc gttgatggtg ttgtagtcaa aattggcggt gaagttctgg
     1621 gcgatcacgt tttcgtcgat gtgccagatc aacagcccgg aaccatccac tggcacgggc
     1681 attccggcag ggacgggccc gcccagaccg ggcagcatga aatcccactc gctgccttta
     1741 tagcggttct ccatgaaatt gaagaaaggc agttcgggga agttttcata gtagtcctgc
     1801 tcgccggggg gcaggagaac gaagctatag ctgggcagat tgttgtaggg attaaggctg
     1861 ccgtcgggat tttgctgtcg gttttccgcc aggaaatact ctctgtccga gatggggatt
     1921 ttataaaccc ggtcgttaac aggcatatgg ttcagaaagt gcgatatgga gaggttttca
     1981 gttgtttccg tgacggtgat gggtgtttcc caaccaagaa aagtgcggca ccaggcactc
     2041 acttgtgccg gaacgtagcc gctggcgttc caaatcccag ttcccatcag gccccagttg
     2101 ccgatgccct gcgaagctcc gttgctggaa tcgttgtcat acaggctggg caggcccagg
     2161 atgtggccaa actggtgggc aagaacgcca ta
//
LOCUS       DAOOWR010000276         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_49943_length_2190_cov_4.435597, whole genome shotgun sequence.
ACCESSION   DAOOWR010000276 DAOOWR010000000
VERSION     DAOOWR010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49943_length_2190_cov_4.435597"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLB85_06475"
     CDS             <1..976
                     /locus_tag="PLB85_06475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU73012.1"
                     /translation="YLGVRHAIGCASGTDALVLAVQALGIGPGDEVITTPFTFFATAS
                     SIWRNGAKPRFADIDPCTFNLDPDKIEAVITPRTKAIMPVHLFGQCCDMERIMEIAHK
                     HGLKVIEDNAQGIGCTWNGKMSCTFGDIGTLSFFPSKNLGAMGDGGMCITNDADLASK
                     LRQLRVHGENPKYYHKWVGLNSRLDTLQAAILDVKLDHLAGWSEARCANAAYYNEKLA
                     SIPQVKTPYIAEEAVSIYNQYTLICEERDSLLEHIKSRDIGCAIYYPKPLHLQECFAE
                     LGYKEGDLPVAERASRQVLSIPIYPELTEAQKLYVFDAIRDFYRGKGE"
     gene            973..1761
                     /locus_tag="PLB85_06480"
     CDS             973..1761
                     /locus_tag="PLB85_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU73013.1"
                     /translation="MKIIVCVKQVPNTTEIRIDPVTNTLIREGVESILNPFDTYAIEE
                     AVRLKETHGGSVTALCMGPPQAEETLREAVSLGVDEIILLSDRRFAGADTWATSLTLA
                     AAISKIGEFDLVFTGQQAIDGDTAQVGPGIAAHLDIRQACFVRKIEELTSCNVTLQRL
                     MEDGYDRLKLKLPAVITVVKEINTPRLPSLRGKRNARAAELKIWNADDLGLDEKTIGL
                     NGSPTQVVKIFTPKHDKQTEKVTVTPDEAADLIIQRLDQITRGG"
     gene            1764..>2190
                     /gene="lgt"
                     /locus_tag="PLB85_06485"
     CDS             1764..>2190
                     /gene="lgt"
                     /locus_tag="PLB85_06485"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU73014.1"
                     /translation="MLQYPDIDPTLVSFSLFGRELAIRWYGFLYVLSFILGYIFYRHM
                     LKIRDLKISRDQYEGAIFSAMLGVILGGRLGYVLFYNLPWYLQHPQEIFFVWEGGMSF
                     HGGALGVIIACLIYMKRQKLSFFKLADPAMPLVAVGLGLG"
BASE COUNT          517 a          661 c          551 g          461 t
ORIGIN      
        1 ctatctgggc gttcgccatg ccatcggctg cgcttccggg actgacgccc tggtcttggc
       61 tgtgcaagcc ctggggattg gccccggcga tgaggtgatt accaccccct tcacattctt
      121 tgccaccgcc tcctccatct ggcgcaacgg cgccaaaccc cgcttcgcgg atatcgatcc
      181 ctgcaccttc aacctcgatc cggacaagat cgaagctgtc atcacgcccc gcaccaaagc
      241 catcatgccg gttcatcttt tcggccagtg ctgcgacatg gaacggatca tggaaatcgc
      301 ccacaagcac ggcctcaagg tgatcgaaga caacgcccag ggcatcggct gcacctggaa
      361 tggaaagatg agctgcactt tcggggacat cggcaccctg tctttcttcc ccagcaagaa
      421 tctcggcgcc atgggcgacg gcggcatgtg catcactaac gatgccgatc tggcctcgaa
      481 actacgccaa ctgagggtcc atggcgaaaa ccccaaatat taccacaagt gggtagggct
      541 gaacagcagg ctggatacgc tccaggccgc gatcctggac gttaagctgg accatttggc
      601 cggctggagt gaagcccggt gtgccaatgc cgcttattac aatgaaaaac ttgcctccat
      661 tccacaggtg aaaacgccct atatcgctga ggaagccgtt tccatctaca accaatacac
      721 cctgatctgc gaagagcgcg actccctgct ggaacacatc aagtcccgcg acataggctg
      781 cgccatttac tatcccaaac cactgcatct gcaggaatgt tttgccgaac ttggctacaa
      841 agaaggcgat ctgcccgtgg ctgaacgagc ctcacgccag gtgctttcaa tacccatcta
      901 ccctgaactc acagaagccc aaaaactcta cgtcttcgat gccatcaggg atttttacag
      961 ggggaaaggt gaatgaaaat catcgtttgc gttaagcaag tgccaaacac caccgaaatc
     1021 agaatcgatc ccgtcaccaa caccctcatc cgggaaggcg tggaaagcat cctcaatcca
     1081 tttgacacct acgccatcga agaggccgtt cgcctcaaag agacccacgg aggcagcgtg
     1141 accgccctct gcatgggccc gcctcaagct gaggaaactt tgcgcgaagc ggtttccctg
     1201 ggcgtggacg agattatttt gctttcagac cggcgtttcg ccggcgcgga tacctgggcc
     1261 accagcctca ccctggctgc cgccatctcc aagatcggcg aattcgacct tgttttcaca
     1321 ggccaacaag ccatcgatgg cgacacagcg caggttgggc cgggtatagc ggcccatctg
     1381 gacatccgcc aggcctgttt tgtgcgcaag atcgaagaac tcacttcctg caatgtaacc
     1441 ctccagcgcc tgatggaaga cggctacgac aggctgaaac taaagcttcc cgccgtgatc
     1501 actgtggtga aagagattaa cacgccccgc ctgccttccc tgcgcggaaa acgcaacgcc
     1561 agggcggctg aactgaagat ctggaacgct gatgacctcg gactggacga aaaaaccatc
     1621 ggcctcaacg gctcgcccac ccaagtagtg aaaatcttca ctccaaaaca tgacaagcaa
     1681 accgaaaaag ttactgtgac ccccgacgaa gccgctgatc tcatcataca gcgtctggac
     1741 caaatcactc ggggaggata accttgctgc aatatcctga catcgatccc accctggtaa
     1801 gcttttccct ttttggtaga gaactggcca tccgctggta cggttttctc tatgtgttaa
     1861 gcttcatcct gggctacatt ttctatcgcc acatgctgaa gatacgggat ttaaagatca
     1921 gccgggacca gtatgaagga gccatcttct ccgccatgct gggtgtgatc ttaggcggtc
     1981 ggttgggata tgtgctgttc tacaatctgc cctggtatct gcagcatccc caagagattt
     2041 tcttcgtctg ggaaggcgga atgagctttc acggcggcgc tttgggcgta atcatcgcct
     2101 gcctcatcta tatgaagcgg cagaaactca gtttcttcaa actggccgat cccgctatgc
     2161 ctctagtagc agtaggtttg gggctcggac
//
LOCUS       DAOOWR010000277         2159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_50970_length_2159_cov_4.206274, whole genome shotgun sequence.
ACCESSION   DAOOWR010000277 DAOOWR010000000
VERSION     DAOOWR010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50970_length_2159_cov_4.206274"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLB85_06490"
     CDS             <1..488
                     /locus_tag="PLB85_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73015.1"
                     /translation="LYFIENSRLVFDPREIALGWLNSTGHKANILSQDFTHEGIAVHL
                     EGNRLYTTQVLALPILKRISALPPAFSAGRSYALEFEYVSVEPREDFNCQLSIPDPNT
                     RVQIDLLTYTLGSMPLSIEWKSENRLILPLEFKYGKGRYSLQLGWDGYYYQDMLEFRA
                     D"
     gene            complement(475..1254)
                     /locus_tag="PLB85_06495"
     CDS             complement(475..1254)
                     /locus_tag="PLB85_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOU73016.1"
                     /translation="MDLHLNLWSWNVNGLRAVMNKDFAATIRREQPDWLGLQETKLQQ
                     HQIPPELEELKGYRFYWSHAERKGYSGTAVLSKIEPLELSYGFGLPEFDNEGRIIRCD
                     YDSFIIYNIYFPNGQMNDERLDYKLRFYDACFERMEADRAGGKMVIVTGDYNTAHHEI
                     DLANPKENEKTSGFLPIERAWLDKIVAAGWLDTFRLFHPEPKRYSWWSYRAGARPRNV
                     GWRIDYFFVNAEARDRVLDADIRDDIMGSDHCPVTLRISLP"
     gene            1447..1581
                     /gene="rpmH"
                     /locus_tag="PLB85_06500"
     CDS             1447..1581
                     /gene="rpmH"
                     /locus_tag="PLB85_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU73017.1"
                     /translation="MKRTYQPSKRSRKNTHGFRSRMATRNGRKVLASRRAKGRKTLCV
                     "
     gene            1585..1911
                     /gene="rnpA"
                     /locus_tag="PLB85_06505"
     CDS             1585..1911
                     /gene="rnpA"
                     /locus_tag="PLB85_06505"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU73018.1"
                     /translation="MLRRITKHSEYREFSSPDLFLRSDHFYAAVLFDPHESALGITIG
                     KKIGKAHTRNLLKRRIKAWARLRCRDFPQGFKLNLIARPGAGELSWNELCAELDKLSA
                     QLWERC"
     gene            1905..2138
                     /gene="yidD"
                     /locus_tag="PLB85_06510"
     CDS             1905..2138
                     /gene="yidD"
                     /locus_tag="PLB85_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOU73019.1"
                     /translation="MLTALLRLPNLSFIGLITFYRKAISPLLPSVCRFQPSCSQYSLQ
                     AFRKYNFFKALGLSAWRVLRCNPFCKGGYDPLP"
BASE COUNT          471 a          637 c          581 g          470 t
ORIGIN      
        1 acctgtattt catcgaaaac agccgcctgg tcttcgaccc cagggaaatc gcccttggct
       61 ggctgaattc gacaggccac aaggccaaca tcctcagcca ggatttcacc catgagggga
      121 tcgccgtgca tttggaaggc aaccggcttt acaccaccca ggtgctcgcc ctgcccatcc
      181 tgaaaaggat ttccgccctg cccccggctt tttctgccgg tcgcagctac gctttggaat
      241 tcgagtatgt ttccgtggag ccgcgcgagg acttcaactg ccaactgagc atcccggacc
      301 ccaacaccag ggtccagatc gatctgctga cctacaccct gggctcaatg cccctaagca
      361 ttgaatggaa gagcgaaaac cggctcatcc tgcccctgga gttcaaatac ggcaagggcc
      421 gctactccct ccagctcggc tgggacggct attactacca ggatatgctg gaattcaggg
      481 cagactgatc ctcagggtaa ccgggcagtg gtctgaaccc atgatgtcgt cgcggatgtc
      541 ggcatcaagc acgcggtccc gagcctcggc attcacaaag aaataatcaa tcctccagcc
      601 cacgttccgg ggccgggctc cggcgcggta gctccaccag gaatagcgtt ttggctccgg
      661 atggaagagg cggaaagtgt ccagccagcc cgcggcgacg atcttgtcca gccaagccct
      721 ttctattggt aagaatccgc tggtcttttc gttttccttg ggattggcga ggtcgatttc
      781 gtggtgggcg gtgttgtaat cgccggtgac gatcaccatt ttgccgcctg ctcggtcggc
      841 ttccatgcgt tcgaaacagg catcgtagaa acggagcttg taatccaggc gctcgtcgtt
      901 catctgtccg ttcgggaaat agatgttata gatgatgaag ctgtcataat cgcagcggat
      961 gatgcggcct tcgttgtcga attccggcag gccgaagccg tagctcagtt ccaggggctc
     1021 gatcttgctc agcaccgccg tgccggaata gcccttgcgc tcggcgtggg accagtaaaa
     1081 gcggtaaccc ttgagctctt ccagctccgg cgggatctgg tgctgctgca gctttgtttc
     1141 ctgcagacca agccagtccg gctgctcacg cctgatggtg gcagcgaaat ccttgttcat
     1201 tacggcccgc aggccattca cgttccagga ccagagattg agatgcaaat ccatcctccg
     1261 cctcggggcg atatttccac ttgggacaag aggtcaccga ggcggttcaa ggctttttgg
     1321 cggggctgaa tctgtcaagt gaaagggcgc gggggagggt gacgtttttt gcgcttgaca
     1381 cttaaagaga actgaaaaaa atggtttatt caatgaaaat gccatacatt ttaaggagtt
     1441 agataaatga aaaggactta tcagccttcc aaacgttccc gcaagaacac ccacggcttc
     1501 aggagccgca tggccacccg caatggccgc aaagtacttg ccagccgccg cgccaaaggg
     1561 agaaaaaccc tctgcgtatg agccatgctt cgtaggatca cgaagcacag tgaataccgg
     1621 gagttcagct cccccgacct gtttctgagg agcgatcatt tctatgctgc ggtgctgttt
     1681 gacccacacg aatccgccct gggcatcacc attggaaaaa agatcggcaa agcgcacacg
     1741 cgcaaccttc tcaaacgcag gatcaaagca tgggcgcgcc tgcgttgccg cgactttccc
     1801 cagggcttca aactcaacct catcgcccgg cccggcgccg gcgaactaag ctggaacgag
     1861 ctttgcgcgg aactggacaa actgagcgcc cagctttggg aaagatgctg acagcgcttc
     1921 tgcgcctgcc gaatctttcc ttcatcgggc tgataacctt ctaccgaaag gccatctcgc
     1981 cgttgctgcc cagcgtctgc cgttttcaac cctcctgcag ccagtacagc ctgcaggctt
     2041 tccgcaaata caattttttc aaagccctcg gcctctcagc ctggcgcgtt ttgcgctgca
     2101 atcccttttg caagggcggt tacgatccgc ttccctaagg agaaaatgtg gataaacgc
//
LOCUS       DAOOWR010000278         2154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_51140_length_2154_cov_2.400191, whole genome shotgun sequence.
ACCESSION   DAOOWR010000278 DAOOWR010000000
VERSION     DAOOWR010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51140_length_2154_cov_2.400191"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLB85_06515"
     CDS             complement(<1..932)
                     /locus_tag="PLB85_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOU73020.1"
                     /translation="MHISAIITIGNEILLGSTINTNLAWLAKELALLGLPVEFSLTVK
                     DEAKAIWQALKHAWESCDVVITTGGLGPTDDDITKREIASFFGAEMRFDAEIWANIQK
                     NFSARNLATPEINRNQALVPEGFKSLTNLRGTAPGLFYEQGGKSFFAFAGVPLEMRHV
                     FSTQAKPLLVAKYGSGEAIHQETLHTFGISESALAELLAGFSPLPEINLAWLPQTGRV
                     DLRFYGRDQEKVTESANNCLKLVGDQVWGCNEDTPVSVLHNLLRKLKLTISVAESCTG
                     GLLQKMLTDPPGASEVFLGGVVSYSNQLKQSLLRV"
     gene            complement(934..>2154)
                     /locus_tag="PLB85_06520"
     CDS             complement(934..>2154)
                     /locus_tag="PLB85_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOU73021.1"
                     /translation="LEDDGHGGLKPAGELTEPYIVRPTSETIIGASFAKWVNSYRDLP
                     LLINQWANIVRWEMRTRLFLRTTEFLWQEGHTVHESEAEAMEETLKMLDVYARFAEDY
                     LAIPVIKGEKTEGEKFPGAVNTYCIEAMMQDRKALQSGTSHFLGQNFAQASNIRFQNR
                     DGEFQHAWTTSWGVSTRLIGALIMAHSDDNGLVLPPKIAPSHIAIIPIYRDEEEHAAV
                     MALANKIDEAFKHTTFEGVRVYTELDDRDLTSSERNWHWIKKGIPLRIEIGPRDVASN
                     SVMVARRDQGPREKQSVAVENVVDHCLQTLGAMQRSILDRALRFREENTMNIDGKEAF
                     YACFTPKNSAQPEIHGGFALSHWCGNSACEEKIKEDLKVTIRCIPFGIKPEAGSCVCC
                     GKPSQGRVIFAKSY"
BASE COUNT          431 a          573 c          617 g          533 t
ORIGIN      
        1 acccgcaaaa ggctttgctt gagctggttt gagtagctga ccacgccacc cagaaacact
       61 tccgaagctc cgggcgggtc ggtcagcatt ttttgcagca agcctccggt gcaggattcc
      121 gccacggaaa tggtgagttt cagcttcctc agcaaattgt gcaaaacact tactggcgtg
      181 tcttcgttgc agccccaaac ctgatcgcca accaatttaa ggcagttatt cgcgctttct
      241 gtcacttttt cttggtctct tccgtaaaac ctcaggtcca ccctgcccgt ctggggcagc
      301 caggccagat taatctccgg aagtggggaa aagcctgcca agagctctgc caaggcggat
      361 tcggagatgc cgaaggtatg cagggtttct tggtggatgg cttctccgct gccgtatttg
      421 gccaccaaga gcggtttggc ttgggttgaa aagacatgcc gcatttccaa aggtactcca
      481 gcaaaggcga aaaagctctt acccccctgt tcatagaaca ggcctggagc cgttccgcgc
      541 aggttggtga gggatttgaa tccttccggc accagggctt gattgcggtt tatctccggt
      601 gtggccaggt ttctggctga aaagttcttt tgtatgttgg cccagatctc tgcgtcgaag
      661 cgcatctccg ctccaaagaa ggaggcgatt tcgcgtttgg tgatgtcgtc gtcagttggg
      721 cccaagccac cggtggtgat caccacgtcg caactttccc aagcatgttt tagggcttgc
      781 cagatggctt tggcttcatc tttcaccgtg agggaaaact ccaccggcag acccagcagg
      841 gcaagctcct tcgcaagcca ggccagattt gtgttgatgg tactgcccag caggatttcg
      901 tttccgatgg tgatgatggc gcttatgtgc atctcaatag gatttggcaa agatcacccg
      961 gccttgggag ggtttgccac agcaaacgca ggagccggct tcaggcttga tgccaaaagg
     1021 aatgcagcgg atcgttacct tcaggtcttc ctttatcttt tcctcgcaag cgctgttccc
     1081 gcaccagtgg gaaagcgcga aaccgccatg gatctcaggt tgggcgctgt tttttggtgt
     1141 aaagcacgcg tagaaagctt ccttgccgtc aatgttcatg gtgttttctt cgcgaaagcg
     1201 cagggcccgg tccagaatgc tgcgctgcat cgcgccaagc gtttgcaggc agtgatccac
     1261 cacgttttcc accgccacgg actgcttttc acggggtccc tggtcacggc gggccaccat
     1321 tacagagttt gaggccacgt ctcgggggcc gatctcgatc cggagcggga ttcccttttt
     1381 gatccagtgc cagtttcgtt cgctggaagt gaggtcgcgg tcatcgagtt ctgtgtaaac
     1441 ccgcactccc tcaaaagtgg tatgtttgaa agcttcgtcg attttgttgg ccagggccat
     1501 aacggcagcg tgttcctctt catcgcggta aatggggatg atggctatat gagagggcgc
     1561 gatcttggga ggcagcacta ggccgttgtc gtcgctgtga gccatgatca gggctccgat
     1621 caagcgggtg gagacacccc aggaggttgt ccaggcgtgc tggaattcac cgtcccggtt
     1681 ctggaagcgg atgttcgagg cttgggcgaa attctgcccc aaaaagtgtg aagtgccgga
     1741 ttgaagagct ttccggtcct gcatcatagc ttcgatgcaa tatgtgttca cggcgccggg
     1801 aaacttctcg ccctcggttt tttcgccctt tattacagga atagccagat aatcctctgc
     1861 gaagcgggca taaacatcca gcattttaag ggtctcttcc atcgcttcag cttcggattc
     1921 atgcacggtg tggccttcct gccagaggaa ttccgtggtg cgcagaaaga ggcgggtgcg
     1981 catttcccaa cgcacgatgt ttgcccactg attgatcagc aggggcaggt cgcggtagga
     2041 attcacccat ttggcaaagg aagcgccgat gatcgtttcg ctggtggggc gcacgatgta
     2101 gggctcagtc agctctccgg cgggcttcaa accgccatgg ccgtcatctt ccaa
//
LOCUS       DAOOWR010000279         2129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_51930_length_2129_cov_4.808100, whole genome shotgun sequence.
ACCESSION   DAOOWR010000279 DAOOWR010000000
VERSION     DAOOWR010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51930_length_2129_cov_4.808100"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLB85_06525"
     CDS             complement(<1..899)
                     /locus_tag="PLB85_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73022.1"
                     /translation="MDKTTKRRLRAVAIFVIFLALSWAGYAALMQKEKGPDYSSLFKD
                     ANKIFGDYKSLNKAQLRVKSSNIKHALQEMNNFISSYALEKPLLKSSESGYGSYIFRI
                     EGGRFSEIQKKFYDLGSIEGYKEIVDSALVIKSLETEEGILASKKSELAAMDAVGQTY
                     GNIADRKEFLISQIRDQENLVKVLSQANTTLLYVQIVPGMTGSSVSTVRKFVYNFFGA
                     LIVLFVAVVLAYYGTKLIMYLMALMGVKGFSASGLGGSYQYGGYGSYTNRYSSRYGYG
                     GSKRKVKRIYKDRRTSNPDSAEKP"
     gene            complement(907..>2129)
                     /locus_tag="PLB85_06530"
     CDS             complement(907..>2129)
                     /locus_tag="PLB85_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOU73023.1"
                     /translation="STQYRGLRESLKELERLKAKVEQDHQLRELYQYQFEELEKAGLK
                     EGEDLELQQEFELLSHAQEIAETAAASSFDLYERENSVHSRLSASVAALARFSHLNPR
                     VANIEQSLRDCLELVTESSAALGDLCQDVNPDPARLETIQERLDTINSLLHKHKAKTI
                     SELLELFAERKAQINAYTDLSERVENLERSTERDFEALRANGDRLSASRLAAAEKLSE
                     ELRESISLLSIPDARFKIKIDKKADGKIIMQEYLDSCTEQGQDACQYFFSANRGSELR
                     PLSTVASGGELSRILLAIKKVLSERIEEKLMILDEIDSGIGGKTAEYVAQFIFALARR
                     HRIICITHLAQIAAIADHQVALKKEAGKKKNVIKMVPLKAEQRLEELARMLSGDVSDI
                     SLEHARELITKYRT"
BASE COUNT          405 a          586 c          550 g          588 t
ORIGIN      
        1 ggcttttccg cgctgtcggg gttggatgtg cgcctgtctt tgtagatgcg ctttactttg
       61 cgtttgctgc cgccgtaacc gtagcgggag gaatagcggt tggtgtagct gccgtatccg
      121 ccgtattggt aagaacctcc caagccgctg gcgctgaatc ctttaacacc cataagagcc
      181 atcaggtaca tgatcagctt ggtgccgtag taggcaagga caacggccac aaacagcacg
      241 atcagggcgc cgaagaaatt atagacgaat ttgcgaacgg tggaaacgct gctgccggtc
      301 atgccgggaa ctatctgcac atagagcagg gtggtattag cctgactcag cactttcacc
      361 aggttttcct gatccctgat ctgcgagatc aggaattcct tcctgtcggc tatgttgcca
      421 taagtttgtc ccacggcatc catcgcggcc aattcactct tcttggaggc caggattcct
      481 tcttccgtct ccagcgactt gatcaccaaa gctgaatcca ctatttcttt atagccttct
      541 atgctgccca ggtcgtagaa tttcttttgg atttctgaaa acctgccgcc ttctatcctg
      601 aatatataag agccatagcc gctttcactg gatttgagca gtggtttttc cagggcatag
      661 ctgctgatga agttgttcat ttcctgcagg gcgtgtttga tgttcgagct cttcacccgc
      721 aactgggctt tattgagtga cttgtaatcg ccgaagatct tgttggcgtc tttgaacagc
      781 gaactgtaat ccggaccctt ttccttttgc atcaaagccg cgtagccagc ccatgacaga
      841 gcgaggaaga tcacaaagat cgccaccgcg cgcagtctcc gtttagttgt tttgtccatt
      901 atatcctcat gtcctgtatt tagttatcaa ttcccgggca tgctccagtg agatgtcgga
      961 tacatctccg gaaagcatcc gggccagttc ttccaatctt tgttccgcct taagcggcac
     1021 cattttaatc acgttcttct ttttgcctgc ctcttttttc agtgccacct gatggtcggc
     1081 gatggcggcg atctgggcca gatgcgttat gcaaatgatc cggtgccgcc gcgccagggc
     1141 gaagatgaac tgcgcgacgt actctgcagt cttgccgccg atgccggaat ctatctcgtc
     1201 caggatcatc agcttttcct ctatccgctc tgacagcacc tttttgatgg ccagcaggat
     1261 gcgggaaagc tcgccgccgg aagccactgt ggacagaggc cttagctcgc tgccccggtt
     1321 cgcgctgaaa aaatactggc aagcatcctg accctgctca gtgcaggaat caagatattc
     1381 ttgcattatg attttaccat ctgccttttt gtcaatcttt attttgaagc gggcgtcggg
     1441 aatggagagc aaactgatgc tttcacgcaa ctcttcggag agtttttccg ccgctgccag
     1501 gcggctggcg ctgagcctgt ccccgttcgc acgcagggct tcaaagtctc tttccgtcga
     1561 tctttccaga ttttcaaccc gctcactcag atcggtgtag gcgttgatct gggccttcct
     1621 ctcggcgaaa agctccagca gttcactgat tgttttggct ttgtgcttgt gcagcagact
     1681 gttgatggtg tccagccgtt cctggatggt ttccaggcgc gcagggtctg gattcacgtc
     1741 ctggcagagg tcacccagcg ccgctgaact ctccgtaacc agttccagac agtcgcgcag
     1801 gctctgttcg atgttcgcca cgcgcggatt gaggtgggaa aaacgggcca gggctgccac
     1861 gctggcgctg aggcggctgt gaacgctgtt ttctctctca tagaggtcaa agctgctggc
     1921 ggcggctgtt tcggcgattt cctgggcatg gctgagcagc tcgaattcct gttgcagttc
     1981 caggtcctcg ccctccttca gtcccgcttt ttccagttcc tcgaactgat attggtaaag
     2041 ctccctcaac tggtggtcct gttccacctt agccttcagt ctttccagtt ccttgaggct
     2101 ctcgcgcagg cctctgtatt gcgtggaaa
//
LOCUS       DAOOWR010000280         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_52443_length_2114_cov_3.433706, whole genome shotgun sequence.
ACCESSION   DAOOWR010000280 DAOOWR010000000
VERSION     DAOOWR010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52443_length_2114_cov_3.433706"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLB85_06535"
     CDS             complement(<1..279)
                     /locus_tag="PLB85_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOU73024.1"
                     /translation="MIRVAIATLGCKTNAYESAVIAAQFPPGEYSAVPFSDEADVYII
                     NTCTVTGRTDFKSRNLIRKALARKAQNPAVKIVVTGCFAQRNPDEIRDL"
     gene            complement(276..884)
                     /locus_tag="PLB85_06540"
     CDS             complement(276..884)
                     /locus_tag="PLB85_06540"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOU73025.1"
                     /translation="MERAASEAWRIAVLTSGHGRGSNLRALYKAFTKNSLPLKIAFAT
                     ASSGKAPVVQLCSDLGLPCHILNPRYQESFENRLLELCRQERIELIALAGFMKLLPPA
                     FLDQAGIPVLNIHPALLPKYGGQGMYGIRVHEAVFDAGERFSGATVHLVDPIYDHGEI
                     IAQEQVDISDCHSPEAIAAKVLEIEHGIYARAIYRFLRRKFT"
     gene            complement(851..>2114)
                     /gene="aspS"
                     /locus_tag="PLB85_06545"
     CDS             complement(851..>2114)
                     /gene="aspS"
                     /locus_tag="PLB85_06545"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOU73026.1"
                     /translation="ILMKSTPEGARDYLVPSRVQPGKFYALPQSPQMFKQLLMIGGFD
                     RYFQIARCFRDEDLRADRQPEFTQLDIELSFVTQEEIFDLLERMLARLFKEVLDIEIQ
                     LPFPRLGYQEAMERFGCDKPDLRFGLELKDLSESLRGSEFQVFKSALEAGGVIKALAI
                     PGGANYSRKQQDELVELARHNGGKGVAFARVADGVLEAGISKFLAPAEAEEIIRITEA
                     ASGDLIAIVADSYDMTAKVLAALRNEVAIRQKLIPENLFSFVWITDFPLFAYNDEEQR
                     WEPAHHMFSLPREEHIPWLDQPDKYGQIIGQLYDLVCNGMELSSGSIRCHRYDLQKKI
                     FDILGFSEDELKERFGFFLEALKYGTPPHGGIAPGIDRLVMIMTGAESMRDVIAFPKT
                     LKAADLMSGAPSEVPEIQWKELHLKPGE"
BASE COUNT          446 a          565 c          621 g          482 t
ORIGIN      
        1 caggtccctg atctcatcag ggttgcgctg ggcaaagcag ccggtcacca cgatctttac
       61 ggcggggttt tgggctttgc gggccagagc cttacggatg aggttgcggc tcttgaaatc
      121 tgtccttcct gttaccgtgc aggtgttgat gatataaacg tcggcttcgt ctgagaaggg
      181 aacggcgctg tattcgccag gcgggaactg ggccgcgatg acagcggatt cataggcgtt
      241 ggttttacag cccagcgtgg cgatggcgac gcggatcatg tgaatttccg acgcaggaac
      301 ctgtaaattg cccgggcgta gattccgtgc tcgatctcta ggaccttggc ggcgatagct
      361 tccggcgaat ggcagtccga gatgtcaacc tgctcctggg caatgatttc gccgtggtca
      421 tagatagggt caaccagatg cacggtggcg ccggaaaagc gctctccagc gtcgaaaaca
      481 gcctcgtgta ccctgatgcc gtacatccct tgtcctccat atttgggcag caaagcggga
      541 tggatgttca gcaccgggat tccggcttgg tcaagaaaag cgggagggag cagcttcatg
      601 aaaccagcca aggcaatcag ttcgatccgt tcctgtcgac aaagctccag caatcggttt
      661 tcaaaagact cctgatagcg cggattaagt atatggcagg gcaatcccag atcagaacag
      721 agctgcacga ccggagcttt gccgctcgaa gcagtggcaa aggctatctt cagcggcaag
      781 ctgttcttgg taaaagcctt gtacagcgcc cgcagattgg agccgcggcc gtgtccgctg
      841 gtaaggaccg ctattctcca ggcttcagat gcagctcttt ccattggatt tcgggcacct
      901 cagaaggcgc gccgctcatg aggtccgccg ccttcagggt tttgggaaag gcgatcacgt
      961 cgcgcatgga ttcagctcct gtcatgatca tcaccaaacg gtctatccct ggcgcaatgc
     1021 cgccatgagg cggggtcccg tatttaaggg cttccaggaa gaagccgaag cgttccttaa
     1081 gttcatcttc gctgaagccc aggatgtcga aaatcttttt ctgcaggtcg tagcggtggc
     1141 agcggatgct gccggaggaa agctccatgc cattgcagac cagatcgtag agttggccga
     1201 tgatttggcc gtatttgtca ggctggtcca gccagggaat gtgttcctca cggggcaggg
     1261 agaacatgtg gtgggccggc tcccagcgct gctcttcatc attgtaggcg aaaagcggga
     1321 aatctgtgat ccagacgaaa gaaaacaggt tttccgggat tagcttttgc cttatagcca
     1381 cttcgtttct aagggctgcc aagaccttag ccgtcatatc gtaagaatct gccacgatgg
     1441 cgataaggtc tccggatgcg gcttccgtga ttcggatgat ttcctccgcc tcagccgggg
     1501 cgagaaactt gctgatgccg gcttccagaa cgccatccgc cactctggca aaggcgacgc
     1561 ccttgccgcc gttatggcgg gccagttcca ccagttcgtc ctgctgtttg cggctgtaat
     1621 tcgcccctcc ggggatagcc agggccttga tcacgccgcc tgcttccagg gcgcttttga
     1681 aaacctggaa ctccgatccc cgcaggcttt cacttagatc cttcaattcc aggccgaagc
     1741 gcaggtcggg cttgtcgcag ccgaagcgtt ccattgcttc ctgatagccc agacgcggga
     1801 aggggagttg gatttcgata tccaggacct ctttgaacaa gcgagccaac atgcgttcca
     1861 gaaggtcgaa aatctcttcc tgggtaacga aactaagttc tatgtccagt tgggtgaatt
     1921 caggctggcg gtcagcccgg aggtcctcat cgcggaagca acgtgcgatt tggaaatagc
     1981 ggtcgaaacc tcctatcatc aggagttgtt taaacatctg gggtgattgc ggcaaagcgt
     2041 aaaatttgcc cggctggacc cggctgggaa ccagatagtc gcgcgcgcct tcaggtgtgc
     2101 tcttcatcaa gatg
//
LOCUS       DAOOWR010000281         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_52488_length_2113_cov_3.213314, whole genome shotgun sequence.
ACCESSION   DAOOWR010000281 DAOOWR010000000
VERSION     DAOOWR010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52488_length_2113_cov_3.213314"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..1228)
                     /gene="pyrF"
                     /locus_tag="PLB85_06550"
     CDS             complement(422..1228)
                     /gene="pyrF"
                     /locus_tag="PLB85_06550"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOU73027.1"
                     /translation="MESIPSASFYCKYQARWRSTKSLLCVGLDSDIAQLPDCVLDEAN
                     PIWEFNRRVIDSTADHACAYKPNLAFYLNDGLRGLEALQRTVEYIPDDIPVILDCKVG
                     DIGNTMEQYAVSYFDRMGFGAITLNPLMGSDVLKPVLKRPGAFAFALALTSNPSAKEF
                     FQEGGMADKVSAWINNYPQEQLGAVVGATQSGDLKRMRELLPGRVFLIPGIGAQGGDL
                     DSVLQNAVDSAETPNILINSSRGIIFREKTPQFAQAAGLAAKELKGLAIV"
     gene            complement(1194..1730)
                     /gene="pyrR"
                     /locus_tag="PLB85_06555"
     CDS             complement(1194..1730)
                     /gene="pyrR"
                     /locus_tag="PLB85_06555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOU73028.1"
                     /translation="MQTKSTIMDKAQMERSVHRMAHEIIEQNRGLDKICLVGIRSRGV
                     PLAKRLSDYLRLIEGKDIPFGILDITLYRDDLSTISHQPVIRGSELGFDIEGAVIVLV
                     DDVLYTGRTVRAAIDALLDFGRPKQIQLAVLIDRGHRELPIKADYVGKNVPTSREEII
                     KVEFEETDGVDSVRIVLL"
     gene            complement(1752..>2113)
                     /locus_tag="PLB85_06560"
     CDS             complement(1752..>2113)
                     /locus_tag="PLB85_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOU73029.1"
                     /translation="RVVRSAPAAPVQATNPAPQPAAAPVQTPNPAPAAPKADPKPAPA
                     SADGTPVQAPMPGMFVKYEKQVGDKVQVGETLLVLEAMKMYNNIPCPVAGTVVATPFS
                     SGDTVGKGDTLIVIKPE"
BASE COUNT          396 a          566 c          667 g          484 t
ORIGIN      
        1 accttcgacc ttcgggttat gagcccgacg agctaccagc tgctccacct cgcgtcaagt
       61 aaataccaga aaattctatg cccttatctt gtcaagggct tttttcgctg cttactactg
      121 cctttttcag gcaggggatt ctccagaaga gcggagctac ataggctggc cgttaaggcc
      181 ctttcctgtg tccaagacgc gcctcccgaa caatacccgc atttgatgcg gggattaggc
      241 gggaggcatg ggagagggat ggaatcaagc gctcagggcc tggccgacag gatatggcgg
      301 tgggtaaaca gttgtggctc tcttttaaat caaggcgata tactatccgc cctcaaatct
      361 acaccattcc gggcctgacc cggaatccgg tttttatcta tcttatggat cccggaccag
      421 gtcagacgat agccaagcct ttgagttctt tggctgccag tccggccgcc tgggcgaatt
      481 ggggagtttt ctcgcggaag atgatgccgc gcgaggaatt gatcaggata ttgggcgttt
      541 cggcggaatc cacggcgttt tgaagcactg aatccaggtc cccgccctgg gcgcctatgc
      601 cgggaatgag gaaaacgcgt ccgggaagca gttcccgcat acgtttgagg tctccgctct
      661 gagtggcgcc caccaccgcg ccgagctgct cctggggata gttgttgatc caggcggaga
      721 ctttatctgc catgccgccc tcctgaaaga attccttcgc ggaggggttg gaggtgaggg
      781 caagcgcgaa ggcaaaagcg ccgggccttt tcaggacagg cttcaggaca tcggagccca
      841 ttagcgggtt gagggtgatg gcgccgaagc ccatccggtc aaagtagctg acggcgtatt
      901 gctccatcgt gttgccgatg tcgccgactt tgcagtccag gatcacgggg atgtcatcag
      961 gtatgtattc caccgtgcgc tgcagggctt ccaggccgcg gaggccgtcg ttcagataaa
     1021 aggccaggtt cggcttgtag gcgcaggcgt ggtccgcggt ggaatctatg acgcggcggt
     1081 taaattccca gatggggttg gcttcatcca gcacgcagtc cggcagttgg gcgatatccg
     1141 aatccaggcc cacgcagagc aggcttttgg tgctccgcca gcgggcctgg tatttacagt
     1201 agaacgatgc ggacggaatc gactccatcg gtttcctcaa attccacctt gatgatctct
     1261 tcgcgggaag tgggcacgtt tttgcccacg tagtctgcct tgatgggcag ttcgcggtgg
     1321 ccgcggtcga tcaggacggc cagttggatc tgtttggggc ggccaaaatc cagcaaggcg
     1381 tcgatggcgg cgcgcacagt tctaccggta tagagaacgt cgtccactag cacgatcacg
     1441 gctccttcta tatcgaaacc cagttctgaa ccgcggatca cgggttggtg tgagatggtg
     1501 gaaaggtcgt cgcggtagag ggttatgtcg agtatgccaa aggggatatc cttgccctcg
     1561 atcaggcgca gatagtcgga aagcctcttg gcaagaggca ctccgcggct gcggatgccc
     1621 accagacaga ttttgtccag gccgcggttc tgttctataa tctcatgtgc catgcggtgg
     1681 acgctgcgct ccatctgggc tttgtccatg atcgtgcttt tggtctgcat cttggctcct
     1741 tgtgcgggga tttattctgg tttgataacg atcagggtgt cgcctttgcc aaccgtgtcg
     1801 ccggaggaga aaggcgttgc cacaacagtt cccgccacgg ggcaggggat gttgttgtac
     1861 atcttcatcg cttccagcac caacagggtt tcgccgacct ggaccttgtc gcccacctgc
     1921 ttttcatatt tcacgaacat tccgggcatg ggggcctgga ccggggtgcc gtcggcagag
     1981 gccggagctg gcttgggatc ggccttgggc gctgccggag cggggttcgg cgtctgaacc
     2041 ggagctgcgg ccggctgggg ggctggattt gttgcctgaa ccggtgcggc tggagcggaa
     2101 cggaccactc tgg
//
LOCUS       DAOOWR010000282         2081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_53541_length_2081_cov_3.867720, whole genome shotgun sequence.
ACCESSION   DAOOWR010000282 DAOOWR010000000
VERSION     DAOOWR010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53541_length_2081_cov_3.867720"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="hydG"
                     /locus_tag="PLB85_06565"
     CDS             <1..1377
                     /gene="hydG"
                     /locus_tag="PLB85_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOU73030.1"
                     /translation="IPTAKIEQLIETRKNAPESRVREIIAKSLDKNRLEPEETAALLS
                     VTDPELKQLILEGAHELKERIYGNRIVLFAPLYVGNECVNDCVYCGFRISNKECVRAT
                     LSREQLVKETQSLVETGHKRLIMVYGEHPMYSPEYIAETVQTVYDTKYAKGEIRRVNI
                     NAAPLDVEGFRTVKSVGIGTYQIFQETYHEETYRHLHPRGPKSNFLWRLDGLDRAMKA
                     GIDDLGIGALMGLYDWKFEVLGLLYHTIHLEETFGVGPHTISFPRIEPAIGTEFADRP
                     PHRVSDEDFKHMVAVIRLSVPYTGMILTAREPIAIRNEILRYGVSQIDAGSSIGVGDY
                     SAHDEESKKKSQFVLGDTRSLDDVIYELAKGGYIPSFCTSCYRAGRTGEHFMEFAVPG
                     FVKRFCTPNALLTFAEYLYDFSSERTLKAGLELIAGETARIEDENMRTAVTAKLEEMK
                     QGKRDLYF"
     gene            1835..>2081
                     /locus_tag="PLB85_06570"
     CDS             1835..>2081
                     /locus_tag="PLB85_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73031.1"
                     /translation="MKSVKSWIVCLVLLCGAAVLMAQSPQWRWATREGLMLTDRGQSI
                     ATDSQGNQYVTGQITTLYGPSPNIFVKKYDPNGNRIWS"
BASE COUNT          481 a          634 c          551 g          415 t
ORIGIN      
        1 atccccacag ccaaaatcga acaactgatc gaaacccgga aaaacgcacc ggaatcccgc
       61 gtccgtgaga taatagccaa atccctggac aagaaccggc tggagcctga agagacggca
      121 gccctgctca gcgtcaccga tcccgaactc aaacagctta tcctggaagg cgcccacgaa
      181 ctgaaggaac gcatctacgg caaccgcatc gtgctctttg ccccgctcta cgtgggcaac
      241 gaatgcgtga acgactgcgt ctattgcggc ttccgcatct cgaacaagga atgcgtgcgc
      301 gccaccctca gccgcgaaca gttggttaag gaaacccagt ccctggtgga aaccggccac
      361 aaacgcctca tcatggttta cggcgagcat cccatgtatt cgccggaata catcgccgag
      421 accgtgcaga ctgtttatga caccaaatac gcgaaaggcg agattcgccg ggtgaacatc
      481 aacgccgcgc ctctggatgt tgagggcttc cgcaccgtga aatccgtggg catcggcacc
      541 taccagatat ttcaggaaac ctaccacgag gaaacctaca ggcacttgca tccgcgcgga
      601 cccaagagca atttcctctg gcgcctcgac ggcctcgacc gcgccatgaa agcaggtatc
      661 gacgacctcg gcatcggtgc cctgatgggc ctctacgact ggaaattcga ggtgctgggc
      721 ctgctttacc acaccatcca tctggaggaa accttcggcg tggggcctca caccatctcc
      781 ttcccgcgca tagagcctgc catcggcacc gaatttgcgg accgcccgcc ccatcgggtt
      841 tcggacgagg atttcaagca tatggtggct gtgatccggc tcagcgttcc ctacaccgga
      901 atgatcctca cggcgcgcga accaattgcc atccgcaacg agattctgcg ctacggcgtt
      961 tcccagatag acgccggttc cagcatcggc gtgggagatt actccgccca tgacgaggaa
     1021 tccaaaaaga aaagccagtt cgtgctgggc gacacccgca gcctggacga cgttatctat
     1081 gaactcgcca aaggcggcta catcccgtcg ttctgcacca gttgctaccg cgctgggcgc
     1141 accggggagc acttcatgga attcgccgtc cccggtttcg tcaagcgttt ctgcactccc
     1201 aacgccctgc tcaccttcgc cgaatacctc tacgatttct cttccgaacg caccctcaag
     1261 gcaggtctgg aactgatagc cggtgagacg gccaggatcg aggacgaaaa catgcgcacc
     1321 gccgttaccg ccaagctcga ggagatgaag caggggaaac gggacctcta tttttaggaa
     1381 cgccctgcga ggcttggggg ggggagcctg gcgtccagcg agcttgcgag catggacggc
     1441 tgggattgtt tttactgctg tcgtcgatgc tcgttcctcg ctctacgcca gcctgccgcc
     1501 cttcaccacg tcattccgcg cccgacccgg aatccagacc gggcggaata gcctataaaa
     1561 ccaccaccca gtcccacacc cacgtcattc cgggcttgac ccggaatcca gtcctattcc
     1621 tgttatggat cccggatcaa tgtcacctcg caagctcggt gacgtccggg atgacgtggc
     1681 ctggagcctg tcgtccatgc tcactatgtt cgctgtacgc caggctacac cagcatcaga
     1741 acttgattca tcggggaggc gaatatttat cttgacacat tttacaatgc cgcgtaaatg
     1801 tgttttagct taattcagcc ttatggaggt aaaaatgaag tcggtgaaaa gttggatagt
     1861 ttgtttggtg ctgctctgcg gagcagcggt tttaatggcg cagtcgcccc aatggcggtg
     1921 ggctaccaga gaaggattga tgctcactga tcgaggccag tccatagcca ccgacagcca
     1981 gggcaaccag tatgtgacgg ggcagattac tactttgtat ggcccaagcc caaatatctt
     2041 tgtcaaaaaa tatgacccaa atggcaacag aatctggagt g
//
LOCUS       DAOOWR010000283         2076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_53736_length_2076_cov_3.303315, whole genome shotgun sequence.
ACCESSION   DAOOWR010000283 DAOOWR010000000
VERSION     DAOOWR010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53736_length_2076_cov_3.303315"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLB85_06575"
     CDS             complement(<1..1275)
                     /locus_tag="PLB85_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73032.1"
                     /translation="MRYVLLCVMALALTGLMFAEVLSCYDVQYTTNPSGASPYAGSVV
                     TVEGIVVAEIFYTGTNTNNRGFMISDPEGGPWSGLLIFTNQYSPQRGDKVQVTGTITE
                     YYDFTEMSPTTGFQVISQGNPLPLPTLITTGSLSGSSAEQWESVFVKVENVNVTSTPN
                     NYGEFNVNDGSGACQIDDQCFPRSGFSWPQMNVGQNWARIQGVVDYSFSSYGINPREM
                     TDLVQIDDVSNATVRVQTTNAEIDTPVDVNIISSRIKPEWGVSSYSAYIRFDPDKVRF
                     NGLKVDETMTAFEPEYWISDAGDSLYVLYQSQEPITSGADDQTLFKLDLTPTSYGESF
                     IQLVSFAYDNTPIQSINSGKLLVPIRSSIAWLNVTNPKNSRNIFNPELNEELTIEYGC
                     KTSAAGINVKAIVRIYDAQGRLVYTPVNTNISN"
     gene            complement(1276..1839)
                     /gene="lspA"
                     /locus_tag="PLB85_06580"
     CDS             complement(1276..1839)
                     /gene="lspA"
                     /locus_tag="PLB85_06580"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOU73033.1"
                     /translation="MERPKRYPAFIIMAAVILLDQVSKMLVRKYIPKGRWITVGEKLW
                     GDFLQICHLENTGAAFSLSLPNPLWNKAFFIGTSVLAVIFILWLLSRATNKVQVVAFG
                     LVLGGAVGNNLIDRPILGAVTDFISFDIPNVIRGMERFPVFNVADSAIFIAMCLLIVD
                     IIFISGKKAPQPETLPDPEINLPDKEQ"
     gene            complement(1843..>2076)
                     /locus_tag="PLB85_06585"
     CDS             complement(1843..>2076)
                     /locus_tag="PLB85_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOU73034.1"
                     /translation="QASDTNLMEQNVMMMEKEREKIRLLNDALRKIYEGFFGICEMCG
                     ENIKESRLEIIPYAKFCIDCMEKMEDKKRRQKR"
BASE COUNT          424 a          547 c          603 g          502 t
ORIGIN      
        1 gttgctgatg ttggtgttta cgggagtgta aaccaagcgt ccctgcgcgt cgtagatgcg
       61 cacgatcgcc ttgacattga tgcccgcggc ggaggtcttg cagccatatt cgatggtgag
      121 ttcctcgttc agttcggggt tgaagatgtt gcgggagttt ttgggattgg tcacattcag
      181 ccaggcgatg ctggagcgga tggggaccag cagtttgccg ctgtttatgg attggatggg
      241 ggtattgtca taggcaaagg aaaccagttg gataaaggat tcgccgtaac tggtgggcgt
      301 gaggtcgagc ttgaagagcg tctggtcgtc ggcgccggag gtgataggtt cctgagactg
      361 gtagagcacg tagagcgaat cgccggcatc cgaaatccag tattctggct cgaaagccgt
      421 catggtttcg tcaaccttca aaccgttgaa ccgaactttg tcagggtcga accggatata
      481 ggcgctgtat gaggaaacgc cccattctgg cttgatacgg gagctgatga tgttcacgtc
      541 cacgggggtg tcgatctcgg cgttggtggt ctggacgcgc acggtggcgt tggaaacgtc
      601 gtcgatctgc acaaggtcgg tcatttcgcg gggatttatg ccgtaactgc tgaaggaata
      661 atccaccacg ccctggatgc gggcccagtt ttgccccacg ttcatctgtg gccaggaaaa
      721 accgctccgg ggaaagcact ggtcgtcgat ctggcaggcg ccgctgccgt cattgacgtt
      781 gaattcgcca tagttgttcg gggtggaggt cacattcacg ttttccacct tcacgaacac
      841 gctttcccac tgctcggcag acgagccgct gaggctgccg gtggtaatca gggtcggcag
      901 gggcagggga ttgccctggc tgatcacctg aaaccctgtg gttggcgaca tttcggtgaa
      961 gtcgtaatac tcggtgatgg tgcctgtcac ctggaccttg tctccgcgct ggggggaata
     1021 ttggttcgta aagatcaaaa gtccgctcca ggggccgccc tcagggtctg agatcataaa
     1081 gcccctgttg ttggtgttgg taccggtgta aaaaatctcg gccaccacaa ttccttccac
     1141 cgtcaccacg ctgcccgcgt agggagaggc gccgctggga ttggtggtgt attgaacgtc
     1201 atagcagctt agcacctccg cgaacatcag tccggttaag gccagcgcca tcacgcaaag
     1261 caaaacgtat ctcatctatt gctccttgtc tggtagattt atctcaggat cgggaagcgt
     1321 ctccggctgg ggtgccttct tcccgctaat aaagattatg tccacgatca gcagacacat
     1381 ggcaatgaaa atcgcgctgt cggcgacgtt gaaaaccgga aagcgctcca tgcccctgat
     1441 cacgttcggg atgtcgaaac tgataaaatc ggtgaccgcg ccgaggatcg gccggtcgat
     1501 gaggttgttt ccgaccgccc cacccagcac caggccgaaa gcgaccacct ggaccttgtt
     1561 ggtggcacgg cttaacagcc agaggatgaa gatcaccgcc agcaccgagg tgccgatgaa
     1621 aaaagccttg ttccaaagcg gattgggcag cgacaggctg aacgccgccc cggtgttttc
     1681 cagatggcat atctgcagaa aatcgcccca cagcttttcc cccaccgtga tccatctgcc
     1741 cttcggaatg tatttccgca caagcatctt gctcacctgg tccaatagga tcacggcggc
     1801 catgatgatg aaggcagggt atcttttcgg ccgttccatg ctctatcttt tctgccgccg
     1861 cttcttgtct tccatttttt ccatgcagtc gatgcagaat ttggcgtagg ggatgatttc
     1921 cagccggctt tccttgatgt tttcgccaca catttcgcaa atcccaaaaa aaccctcgta
     1981 gattttgcgc agggcgtcgt taagcaggcg gatcttttcg cgctcctttt ccatcatcat
     2041 cacgttctgt tccatcaggt tcgtgtccga ggcctg
//
LOCUS       DAOOWR010000284         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_53848_length_2073_cov_3.203667, whole genome shotgun sequence.
ACCESSION   DAOOWR010000284 DAOOWR010000000
VERSION     DAOOWR010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53848_length_2073_cov_3.203667"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="mutS"
                     /locus_tag="PLB85_06590"
     CDS             complement(<1..1607)
                     /gene="mutS"
                     /locus_tag="PLB85_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU73035.1"
                     /translation="MKKSTTPDARLTPMLRQFQEVKQAHPDKLILFRMGDFYETFFDD
                     AHTAARILNITLTSRDKNSENPVPLAGFPYHALDSYLDKLIQAGLKVAICEQTEDPKQ
                     AVGLVKREVVEIITPGTVLDHALISGSANNFLAAVWYENEKRRIGLANLDLSTGEFSF
                     TELSGDELVNELLRLKPSEIIVDGSASEAFLKSLKLEYSPTITIFDSWQFRPSEAASI
                     LKKHFQVHSLEAFDAQGRTAGATAAGAALAYVQSLQQTPLTHISSLRYYSLSNFMQLD
                     EISRRNLELIRSIRYGERHGSLLAILDQTQTAMGSRLIQSWLLHPLLDTVEIVKRQNL
                     IKAFLTNTAYLKDVRSSLHEIGDVSRLVSKLGALRINPRELIALRSYLLVLGELKERL
                     QAYNQPLITALADDLEGFDALADAIGAALRPQPPLTITEGGIFNPGYNKDLDELLELV
                     HDGKSWIARLEDDERKKTGISTLKVGYNRVFGYYLEVGTAHKSKVPDYYVPKQTLVNS
                     ERYISPRLKEFEAKVLSSEEKIKNLEY"
     gene            complement(1616..>2073)
                     /locus_tag="PLB85_06595"
     CDS             complement(1616..>2073)
                     /locus_tag="PLB85_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU73036.1"
                     /translation="NYQTGFEINRKSQLAFEIEDRLFELKGRYGSQVDLSFLYPYTEL
                     DLPELVTEPEVQPLPALAEADLNSPAKLEAKPAGTFVQAGRFGKEENARNLTDVIRAM
                     KLKANYFEDRGNKSVPWVVVSGPYASQSEANSVAGILRANSIECFVTRF"
BASE COUNT          392 a          517 c          677 g          487 t
ORIGIN      
        1 tcgtattcga ggtttttgat cttctcttcc gaactgagca ctttagcctc gaattcctta
       61 agccggggcg aaatgtagcg ttcggaattc accagggtct gttttggcac atagtaatcc
      121 ggcactttgg atttgtgggc ggtgccgacc tccagatagt agccgaagac gcggttgtag
      181 cctactttga gggtgctgat gcctgttttc ttgcgctcgt cgtcctccag gcgggcgatc
      241 caggatttgc cgtcgtggac aagttccagc agttcgtcca gatccttgtt gtagcctgga
      301 ttgaagatgc cgccttcggt gatggtgagg ggcggttgtg gacgtagggc ggcgccgatg
      361 gcatcggcca gggcgtcgaa accctccaaa tcgtctgcca aagcggtgat gaggggttgg
      421 ttgtaagcct gtagccgctc tttcagttcg cccaacacca ggaggtagga gcgcagggcg
      481 ataagctcgc gggggttgat gcgcagggcg cccagcttgg aaacgaggcg ggaaacgtcg
      541 ccgatctcgt gcaggctgga gcgcacgtcc ttgaggtagg cggtgtttgt caggaaggcc
      601 ttgatcaggt tttgcctttt gacgatctcg actgtatcca gcaggggatg gagcagccag
      661 ctctggatga ggcgcgagcc catcgcggtc tgggtttggt ccagaatcgc cagcaagctg
      721 ccgtggcgtt cgccgtagcg gatggaacgg atgagttcaa ggttgcggcg gctgatttcg
      781 tcgagctgca tgaagttgct cagggagtaa tagcgcagcg agctgatgtg ggttaggggc
      841 gtctgctgaa gcgattgcac ataggcaagg gctgccccgg cagcggtggc cccggctgtt
      901 ctgccctggg cgtcaaaggc ttccagggaa tgcacctgga aatgtttttt caggatggaa
      961 gcggcttccg aaggccggaa ctgccagctg tcgaatattg tgatggtggg cgagtattcc
     1021 agcttgaggc ttttgaggaa ggcctcggag gcgctgccgt ccacgatgat ctcgctgggc
     1081 ttaaggcgca aaagctcgtt cacaagctcg tccccgctca gttccgtgaa gctgaattca
     1141 ccggttgaga ggtcgagatt ggccaggccg atccggcgtt tttcgttttc ataccagacg
     1201 gcggccagga agttgttcgc gctgccggag atgagggcat ggtcgagcac ggttcccgga
     1261 gtgatgatct ccaccacttc gcgcttcacc agccccacag cctgtttggg gtcttcggtc
     1321 tgctcgcaga tggccacctt gaggccagcc tggatgagct tgtccagata gctgtccagc
     1381 gcgtgatagg ggaaaccggc caggggaacg gggttttcgc tgtttttgtc gcgggaggtg
     1441 agggttatgt ttaggatgcg ggcagcggtg tgggcgtcat caaaaaaggt ctcgtaaaaa
     1501 tcgcccatgc gaaagaggat aagcttgtcc ggatgggctt gcttcacctc ctgaaattgc
     1561 ctcagcatgg gggtcagcct ggcatccggg gtggtggact ttttcattta aggtttcaaa
     1621 aacgggtgac gaagcattcg atggaattgg cgcgcaggat gccagccacg ctgttcgctt
     1681 cgctttgaga ggcgtaggga ccgctcacca ccacccaggg cacggatttg ttgcccctgt
     1741 cctcaaagta gttggctttc agcttcatag ctcggatcac gtccgtaagg ttgcgggcgt
     1801 tctcttcttt gccaaagcgt cccgcctgca caaaggttcc ggcaggtttg gcctccagct
     1861 tggccgggga attaagatct gcttccgcca gagccggcaa gggctgaacc tctggctcgg
     1921 taacaagttc cggaagatcc aattccgtgt agggatagag gaatgaaaga tcgacctgcg
     1981 agccgtatct gcccttgagt tcaaagaggc ggtcctcgat ctcgaaagca agctgggact
     2041 tgcggttaat ctcaaacccg gtctggtagt tgg
//
LOCUS       DAOOWR010000285         2068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54015_length_2068_cov_4.267760, whole genome shotgun sequence.
ACCESSION   DAOOWR010000285 DAOOWR010000000
VERSION     DAOOWR010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54015_length_2068_cov_4.267760"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..276)
                     /gene="xseB"
                     /locus_tag="PLB85_06600"
     CDS             complement(37..276)
                     /gene="xseB"
                     /locus_tag="PLB85_06600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU73037.1"
                     /translation="MNESNGNPLLNFEKSLRSLEATVERLQQEQLDLDLMVKLYEEGI
                     THLEACQKALEGAELKIRQLNTRIKAGENEGNENG"
     gene            complement(323..973)
                     /locus_tag="PLB85_06605"
     CDS             complement(323..973)
                     /locus_tag="PLB85_06605"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOU73038.1"
                     /translation="MKSGDSTAWLFTATSPAEIFGGYASIDPNRDLMIAVDSGLERVR
                     VLGLKPDLIIGDMDSVEPELLRLYPDTTQLRHPADKNETDTELALLWCLNAKVGEIVI
                     CNGLEGRFDHSLALVQNLEFLQTQGIPARIESARQKVWFLSPETTIRACRGCQLSLLA
                     WSGEAKFDDSSGLKYPLDGISLFPQKVRGMSNQVDKEEATIKLASGIILAILTKSC"
     gene            complement(973..1908)
                     /locus_tag="PLB85_06610"
     CDS             complement(973..1908)
                     /locus_tag="PLB85_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU73039.1"
                     /translation="MKEDKKLRELQPEIFPDRRIKFTVDNMGSKHSLALRPWVLVLVA
                     VLLLGGLTLLLIFGGKGSGAADAELLAKLEKENKFLREKVDSYEAELDSVMATLDTLN
                     INVPSEAFSYPSYSDEPFGPENKLQVHPGLEKKLIGIEIKLANIKQRLGFALEESKGD
                     FKLPYGFDRHGDGIPSIQPTFGEIVSGWGIRLHPVLGDYRFHQGIDIANEIGTPVYAT
                     ADGVVERAQTENGWGKMVSIIHTDGYRTHYAHLHNIKVRVGDVVAKGQIIGLMGSTGM
                     STGPHLHYGVYRDNQSLNPVYYLNRADTNTFASKG"
BASE COUNT          413 a          566 c          549 g          540 t
ORIGIN      
        1 tgtttctctt tcatgtcctt tttcaggcgc acttttctat ccattttcat tcccctcgtt
       61 ctcaccggcc tttatcctgg tgttaagctg cctgattttg agttcagccc cttccagcgc
      121 tttctggcag gcttccagat gcgtgattcc ctcctcgtaa agtttcacca tcaggtccag
      181 gtccagttgt tcttgctgca aacgctccac agttgcctca agagaacgca gcgacttctc
      241 gaagttcaaa agcgggtttc cgttgctttc gttcatgttt ttcctccaat ggttgaaacg
      301 cttaatcgtg tcataagata atttagcatg atttagtcaa tatagccaga attattccgc
      361 tggcaagttt gatggtggcc tcttctttat ccacctgatt gctcatcccg cgcacttttt
      421 ggggaaacaa ggaaataccg tcaaggggat atttgaggcc ggaggagtca tcgaacttcg
      481 cttccccgct ccaggccagc agggaaagct ggcagccccg acaagctcgg atcgtggttt
      541 cagggctcag gaaccagact ttttgccggg cggattcgat tctggctgga atgccttggg
      601 tttgcaggaa ttccaggttt tgcaccaagg ccaggctgtg gtcgaagcgc ccttccagac
      661 cgttgcagat aacgatctcc cccactttgg cattgagaca ccagagtagc gcgagttctg
      721 tgtcggtctc gtttttatcg gcaggatggc gcagttgtgt ggtgtcggga tagagtcgca
      781 gaagttcagg ttccaccgaa tccatgtccc cgattatcag gtcaggcttc agaccaagga
      841 cccgaaccct ctccaaaccg gagtcaacgg cgatcatcag gtcccggttt gggtcgattg
      901 aggcatagcc tccaaaaatc tctgctgggc tggtggccgt gaacagccag gcggtggaat
      961 cccctgattt catcagcctt tggaggcgaa ggtgtttgta tccgcgcggt tcagataata
     1021 aaccgggttc agggattggt tgtcgcggta caccccgtaa tgcagatgag gccctgtgga
     1081 catgcctgtg ctgcccatca ggccgatgat ctggcctttg gcaaccacgt cgcccaccct
     1141 caccttgata ttgtgtagat gtgcgtaatg cgtcctgtat ccgtctgtgt ggatgatgct
     1201 taccattttc ccccagccgt tttcagtctg ggcgcgttcc accactccgt cggcagtggc
     1261 atagacaggt gtgccgattt cattcgcgat gtcgatgccc tgatggaagc ggtagtcgcc
     1321 caaaaccgga tgcaggcgga ttccccatcc gctcacgatt tcgccgaaag tgggctggat
     1381 ggaggggatg ccgtctccgt ggcggtcaaa gccataggga agtttgaaat cccccttgct
     1441 ttcctccagc gcgaacccca ggcgctgttt gatattggcc agcttgattt cgatgccgat
     1501 gagtttcttt tccagcccgg gatgcacctg cagcttgttt tcggggccga agggttcatc
     1561 gctgtaagag ggataggaaa acgcttccga gggcacattg atgttcaggg tgtccaaagt
     1621 tgccatcacg gaatccagtt ccgcttcata gctgtccacc ttttcccgca ggaacttgtt
     1681 ttccttttcc agcttggcga gcaattccgc gtctgctgct ccgcttccct tgccgccaaa
     1741 aatcagcagc agcgtcaaac cgccaagcag cagcaccgcc accagaacca gcacccaggg
     1801 acgcagggcc aggctgtgtt tgctgcccat gttgtccacc gtgaacttga tccggcggtc
     1861 cggaaaaatc tccggctgca actcgcgcag ttttttgtct tccttcattt atctgcgatc
     1921 tctctatgta atctattgag cgggaaaccg atggcaaata ctatccggat gaactgagcg
     1981 ctggggaagt ggatgtcggt cggaggccaa agaatggcat cccgtagggg aatcgaaccc
     2041 ctgttgcgtg actgagaatc acgagtcc
//
LOCUS       DAOOWR010000286         2059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54337_length_2059_cov_3.552894, whole genome shotgun sequence.
ACCESSION   DAOOWR010000286 DAOOWR010000000
VERSION     DAOOWR010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54337_length_2059_cov_3.552894"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PLB85_06615"
     CDS             complement(<1..500)
                     /locus_tag="PLB85_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOU73040.1"
                     /translation="MDDKTKILNVQIEDHLRQAYLDYSMSVIVSRALPDIRDGLKPSQ
                     RRIIYAMSELNLSPGGHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWNMRYPLV
                     DGQGNFGSIDGDPPAAMRYTEARMQKVTMEMLEELDKDTVDFKGNYDDTRKEPEVFPS
                     RIPNLL"
     gene            complement(518..>2059)
                     /locus_tag="PLB85_06620"
     CDS             complement(518..>2059)
                     /locus_tag="PLB85_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HOU73041.1"
                     /translation="FSVVVALSEYLEARVHTGGKLYFQRYERGQVVTEVKVLGETNRT
                     GTVIKFKPDPTIFETVEFSFDYLTTRLRELSYLNRGVRIILKDERSDRIHDFHFEGGI
                     ESFVEHLNRNKKPLGSKPFYISAERKGMELELAMQYNEGYQENIFSYANNINTTEGGT
                     HLSGFKSGLTRAVNTYIKNADLLKNEKVTPSGEDIREGLTTVISVKLSNPQFEGQTKT
                     KLQNSEVDGFVNSVVYEKLMTYFEEHPAEAKTITLKSILAARSREAARKARELTRRKS
                     VLESGSLPGKLADCTITDPAKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKMLNTE
                     KARVDKVLNNDKIQPIILAIGAGVGQDFDVSKIRYGSVVIMADADVDGAHISTLLLTF
                     FYRYMKPLIEHGHVYIAKPPLFLVRKGKQKKYVFSEEERDAAIAELGEKGVVVQRYKG
                     LGEMNPDQLWETTMDPENRIMISVKMDDAIEADRMFTVLMGDEVEPRREFIQANAKYV
                     QNLDI"
BASE COUNT          356 a          553 c          611 g          539 t
ORIGIN      
        1 atcagcaggt tggggatgcg ggaggggaaa acttcaggct ctttgcgcgt gtcgtcatag
       61 ttgcccttga aatccaccgt atccttgtcc agctcttcca gcatctccat ggtgactttc
      121 tgcatccggg cctcggtgta gcgcatggct gcggggggat cgccgtcgat ggagccaaag
      181 tttccctgcc cgtccacgag cggatagcgc atgttccaag gttgggcgag gcgcacgagg
      241 gtgggataaa ccacctgttc gccgtgggga tggtagtttc cggaggtatc gccggctatt
      301 ttggcgcatt tgcggaaatg accgccgggc gagaggttca gctcgctcat cgcgtatatt
      361 atgcgtctct gggaaggctt gagtccgtca cggatgtcag gcaatgcacg ggacacgatc
      421 acactcatgg aataatccag ataggcctgc cgcagatggt cttcaatctg tacgttcagt
      481 atttttgttt tatcgtccat tcttcactcc agctttttta gatatccagg ttctgcacgt
      541 atttggcgtt ggcctggatg aattcgcggc ggggctcgac ctcgtcgccc atcaggaccg
      601 tgaacatcct gtcggcttcg atggcgtcgt ccatcttcac cgaaatcatg atccggtttt
      661 cggggtccat ggtggtttcc caaagctggt ccgggttcat ttcacccaag cctttgtagc
      721 gctggaccac cacccctttt tcgcccagtt ccgcgatcgc ggcgtcgcgc tcttcctctg
      781 aaaacacgta tttcttctgc ttgcccttgc gcaccagaaa gagaggcggt ttcgcgatgt
      841 aaacgtgacc gtgctcgatg aggggcttca tatagcggta gaagaaggtg agcagaaggg
      901 tgctgatgtg ggctccgtcc acgtccgcgt ctgccatgat gacgacgctg ccgtagcgta
      961 tcttactcac gtcaaaatcc tggccgaccc cggcgccaat ggccaggatg ataggctgga
     1021 ttttgtcgtt gttcaaaacc ttgtccactc gtgccttttc ggtgttcagc atcttgcccc
     1081 agagcggcag gatggcctgg aaagagcggt cccggccctg tttggcactg cctcccgcgg
     1141 agtctccctc caccaggaaa agctcggttt tggcggggtc ggtgatggtg cagtcggcta
     1201 gttttccggg aagcgagccg ctttccagca cggatttccg ccgggtgagt tccctggctt
     1261 ttctggcagc ttcacgggaa cgggcggcga ggatgctttt cagggtgatg gttttggcct
     1321 cggcgggatg ttcctcgaaa taggtcatca gcttttcata gaccacagag ttcacgaagc
     1381 cgtccacttc ggagttttgc agcttggtct tggtctggcc ttcgaactgg gggttgctca
     1441 gcttcacgct gatcaccgtg gtgaggcctt ctcggatgtc ctctccggag ggcgtaacct
     1501 tctcgttttt taacagatcg gcgttcttga tataggtgtt cacggcgcgg gtgaggccgc
     1561 ttttgaagcc gctgaggtgc gttccgccct cggtggtgtt gatattgttc gcgtagctga
     1621 agatgttttc ctggtagcct tcgttgtatt gcatcgccag ttccagttcc attcccttac
     1681 gctcggcgct gatgtagaag ggcttgctgc cgaggggttt tttgttcctg ttcaggtgct
     1741 ccacgaaact ctcgatgccg ccttcgaaat ggaagtcgtg tatccggtcg ctgcgctcgt
     1801 ccttcaaaat gatgcgcacc ccgcggttga ggtaggaaag ctcgcgcagg cgggtggtga
     1861 ggtagtcgaa gctgaattcc accgtctcaa agatggtggg gtcaggcttg aattttatca
     1921 cggtcccggt acggttcgtt tcgcccagaa ccttcacttc ggtgacgacc tggcctctct
     1981 cgtagcgctg gaaatagagc tttccgcctg tgtggacacg cgcttcgagg tattcggaaa
     2041 gcgccaccac cacggaaaa
//
LOCUS       DAOOWR010000287         2052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54599_length_2052_cov_3.539309, whole genome shotgun sequence.
ACCESSION   DAOOWR010000287 DAOOWR010000000
VERSION     DAOOWR010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54599_length_2052_cov_3.539309"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLB85_06625"
     CDS             complement(<1..1056)
                     /locus_tag="PLB85_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOU73042.1"
                     /translation="MYKEPYSFNIARYMEPERFARFREFAGTQETPVLIVDLDIIKAK
                     YLELQQSIPNLQIYYAVKANPMNEVIRLLDGLGSNFDVASVNELDQLLQLGIDPKKMS
                     YGNTIKKARDIRYFHEHGVPMFVTDSELDLQRIAENAPGAKVYFRLLTEGTGADWPLS
                     RKFGAHPDVIYNLILKAPELGLVPWGVSFHVGSQQRDIGQWDDAIARAKYIFDAVLEE
                     GIELQMINLGGGYPASYVDPTYSIEEYSSEIKRFIEEDFGEHIPQLVMEPGRSLVADS
                     GVMVAEVVNIATKAKNNLYKWVFLDVGKFGGLIETIDESIRFPIFFEREGLANEIIMA
                     GPTCDSMDIMYERAKVPM"
     gene            complement(1157..>2052)
                     /locus_tag="PLB85_06630"
     CDS             complement(1157..>2052)
                     /locus_tag="PLB85_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73043.1"
                     /translation="LAGISTETLERNDYRLRQAFIHFRSGRHVETDSLLRAVIADSLM
                     LERKNYQRRGVNLNARKLMAENSLALTKSTATAKTWYEEARRFCGSVYDRQNIDLALV
                     RLLAIDQDFETALAILNGVNEPKHLETRDYLRFSVELLRGNTDVADSLMNEYVIRWPG
                     GVYVNDAIYQMMFVLELSGKDLDSFHLANRMMLLGDPAAVDTLAAVFASTDDEELLIL
                     AVEWAILLAEPDKALNLLEHDWQDPVSAEYAALLRLKLSKAEDEAQRFARDFLTANPG
                     SIFAPKFRMSLARTGYSRPDF"
BASE COUNT          381 a          567 c          619 g          485 t
ORIGIN      
        1 catgggcacc ttggcgcgtt cgtacatgat gtccatgctg tcgcaggtgg ggccggccat
       61 gatgatttcg ttggccaggc cttcgcgttc gaagaagata gggaacctga tcgattcgtc
      121 gatggtctcg atcaggccgc cgaatttgcc cacatccaga aacacccatt tgtaaaggtt
      181 gttcttcgct ttggtggcta tattcaccac ttcggcgacc atcactccgg aatcggcaac
      241 cagcgaacgc cccggttcca tcaccagttg cgggatgtgt tcgccaaagt cctcttcgat
      301 gaagcgcttg atctcggagc tgtattcctc tatggagtag gtcgggtcaa catagctggc
      361 cggatagccg ccgccgaggt ttatcatctg gagctcgatg ccctcttcca gcacggcgtc
      421 aaagatgtat ttggcccgcg cgatggcgtc gtcccactgc ccgatgtcac gctgctgcga
      481 acccacgtgg aaagatactc cccaggggac gaggcccagt tccggggctt tgaggatgag
      541 gttgtagata acgtccggat gggcgccaaa cttcctggaa agcggccagt cggctcccgt
      601 gccttcagtc agcaggcgga aatagacctt ggcgccgggc gcgttttccg ctatgcgctg
      661 caagtccagt tcgctgtcag ttacgaacat ggggacgcca tgctcgtgga aatagcggat
      721 gtccctggcc ttttttatgg tgttgccata gctcattttt ttggggtcga tgcccaactg
      781 gagaagctgg tccagctcat tcacggaggc gacgtcgaag tttgagccca ggccatccag
      841 caggcggatc acttcgttca tcggattggc tttcacggcg tagtagattt gcaggttggg
      901 aatgctttgc tgaagctcca gatatttggc tttgatgatg tccaggtcca cgatcagcac
      961 aggtgtctcc tgggtgccgg caaattcgcg gaaacgggca aagcgttccg gctccatgta
     1021 gcgggcaata ttgaagctat acggctcttt gtacattggg aagtcctctg ttcagttgat
     1081 ggtgctcaaa aatccagtgg cttaaaatgt gtcaaccttt cttgcccggc acagatggac
     1141 ccagctctcg tggcggtcag aagtctggac ggctgtagcc ggtgcgggca aggctcatgc
     1201 ggaattttgg ggcgaagatg ctgccgggat tggcggtgag gaagtctctg gcaaatcgct
     1261 gcgcttcatc ttccgccttg ctgagcttca ggcgcagcaa ggccgcgtat tcggcgctca
     1321 cggggtcctg ccagtcatgt tccagcaggt tcagggcctt gtccggctcg gccagcagaa
     1381 tggcccattc caccgccagg atgagcagtt cctcatcgtc cgtggaggca aaaaccgcag
     1441 ccagggtatc cacggcggcg ggatcgccca gcagcatcat ccggttagcg agatggaagc
     1501 tgtccaggtc ttttccgctt agttccagca cgaacatcat ttggtagatg gcgtcgttca
     1561 cgtaaacccc accgggccag cggataacgt attcgttcat caggctgtcc gcgacgtctg
     1621 tattgccccg cagcagttcc acgctgaagc gcaggtagtc gcgggtttcc aggtgtttgg
     1681 gctcgttcac gccgttcagg atggccaggg cggtttcgaa atcctgatca atcgccagca
     1741 ggcgcaccag ggccaggtct atattttggc ggtcatagac gctgccgcag aatctgcgcg
     1801 cctcttcata ccaggttttg gcggtggcgg tgcttttggt cagggcgagg ctgttttccg
     1861 ccatcagctt gcgggcgttc aggttcacgc ccctgcgctg gtagtttttc cgctccagca
     1921 tcagcgaatc ggcgatcacg gcccgcagca ggctgtccgt ttccacgtgg cgcccggaac
     1981 ggaagtggat gaaagcctga cgcaggcggt agtcgttcct ttctagggtt tccgtgctga
     2041 tgccggccag at
//
LOCUS       DAOOWR010000288         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54868_length_2044_cov_4.638009, whole genome shotgun sequence.
ACCESSION   DAOOWR010000288 DAOOWR010000000
VERSION     DAOOWR010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54868_length_2044_cov_4.638009"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..>2044
                     /locus_tag="PLB85_06635"
     CDS             277..>2044
                     /locus_tag="PLB85_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOU73044.1"
                     /translation="MNNYFMETHHHEWILTNKLGSYALGTGNLINQRKYHGLLIASDR
                     NFSRFHLVAGMEERVEWRGQILHLDSNNYSNCIYPEGFLYLVKPWLRPFPTFLYSALP
                     HQNAILIRKEIKMDADTNAVIVKYTNLGHHKLHFTLHPKFTMVPHHELNHPGSLDHER
                     FDTEFENSGEGCSFSATRAANGLRVWGMMKGAELTPNRFTYNNVFYPWEVMSGYAGIG
                     DQISLFELNFALDIGESKILLFADQPIQAPDKTIARIEKRYANLPKPKDFPREPDSDD
                     TLIESLDLNDNFLYSYEEYLRLLEFALRDFVSNNDIVAGYPFYGAWGRDTMIVLNALM
                     HTPGNLKLAEKVLMRYRREMKDGLIPNMRAESGREANYDTVDATLWFVILMWKLGRYK
                     QDKAFWRGSIKLIEEVLTGILNNSQYPFFVRPDGLIELKEEFAHATWMDVRIDGKPVT
                     PRNGAPVEINALWYNAVCCYQAMCDEYRRLAKVRYLPIEQITDMKDLIRASFQKFWSQ
                     GYLADRLLGDNPVDEMRPNAVIALSLPWEIIDRDKMQQVWNRAREELYTPYGLRSLSP
                     NNFRFRKKYYGTQRERDLSYHNG"
BASE COUNT          544 a          593 c          547 g          360 t
ORIGIN      
        1 tttcgggagg cgcatcagac agcaaataga gtgtgttaac gaacggcaag gaagaggttc
       61 acgcggattc agcggatatc aaggattcta catatagcat gcagctacac tccttgaagc
      121 aaaaaccagg gggaaagggc aaaattagat gaagggaaag aagaaaaaag ttggtgcggg
      181 gttgcaatgg aaaaaagtca acaaaagact tgacggaatc aggggggttg aaataattca
      241 acaatcaaga ataatacaac tgaccaaggg ggttcagtga acaactattt catggagacc
      301 catcaccacg agtggatcct gaccaacaag ctggggtcct acgctttggg taccggaaac
      361 ctgataaacc agcgcaaata ccacgggctg ctaatcgcca gcgacaggaa tttttcgcga
      421 ttccacctgg tcgcggggat ggaggaaagg gtggagtggc gcggccagat cctgcatctg
      481 gacagcaaca actacagcaa ctgcatctat cccgagggat ttttgtatct ggtgaaaccc
      541 tggctacggc ccttccccac ctttctctat tccgccctgc cgcatcagaa cgccatcctg
      601 atccgcaaag agatcaagat ggatgccgat accaacgccg ttatcgtgaa atacacgaac
      661 ctggggcatc acaagctgca tttcaccctg catcccaaat tcaccatggt gccacaccac
      721 gagctgaacc acccgggcag cctggaccac gagcggttcg acacggaatt tgaaaacagc
      781 ggggaaggct gttccttcag tgccacccgc gccgccaacg gcctgcgggt ctgggggatg
      841 atgaaagggg cggagctaac gccaaaccgg tttacctata acaacgtttt ctacccctgg
      901 gaagtgatga gcgggtatgc cggaatcggc gaccagatca gcctctttga gctgaacttc
      961 gccctggaca tcggcgaaag caaaatcctg ctcttcgctg accagcccat ccaggcgccg
     1021 gacaagacaa tcgccaggat cgaaaagcgc tacgccaatt tgcccaagcc caaagacttc
     1081 ccgcgggaac cggacagcga cgacaccctg atcgaaagcc tggacctgaa cgacaacttc
     1141 ctctacagct atgaagaata cctgcggctg ctggaattcg cgctcaggga tttcgtctcc
     1201 aacaacgaca tcgtggcagg ctatccattt tacggcgcct ggggacgcga caccatgatc
     1261 gtgctcaacg ccctgatgca cacccctggc aacctgaagc tggcggaaaa ggtgctgatg
     1321 cgctacagac gcgaaatgaa ggacggcctg ataccgaaca tgagagccga atcaggaagg
     1381 gaagcgaatt acgacaccgt ggacgccacc ctgtggttcg tcatcctgat gtggaaactg
     1441 ggccgctaca aacaggacaa ggccttctgg cgcggaagca tcaagctcat cgaggaagtt
     1501 ctgacaggca tcctcaacaa cagccaatat cccttctttg tgcgcccaga cggcctgatc
     1561 gaactgaaag aggaattcgc ccacgccacc tggatggacg ttcggatcga cggaaagccc
     1621 gtgacgccga ggaacggagc ccccgtcgag atcaacgccc tctggtacaa cgccgtctgc
     1681 tgttaccagg ccatgtgcga cgaataccgc cgcctggcca aggtccgcta cctgccgatc
     1741 gagcaaatca ccgacatgaa ggacctgatc cgcgcctcct tccagaaatt ctggtcccag
     1801 ggctatctgg cggaccgcct gctgggtgac aatcccgtgg acgagatgag gccgaacgcc
     1861 gtgatcgcgc tttccctgcc ctgggaaatc attgaccgcg acaagatgca gcaggtttgg
     1921 aaccgcgccc gcgaagagct ttacacgccc tacggcctgc ggagcctgag tccgaacaac
     1981 ttccgcttcc gcaaaaaata ctacggcacc cagcgcgaac gcgacctctc ctaccacaac
     2041 ggca
//
LOCUS       DAOOWR010000289         2044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54874_length_2044_cov_3.975365, whole genome shotgun sequence.
ACCESSION   DAOOWR010000289 DAOOWR010000000
VERSION     DAOOWR010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54874_length_2044_cov_3.975365"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLB85_06640"
     CDS             complement(<1..862)
                     /locus_tag="PLB85_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOU73045.1"
                     /translation="MKNILVLGAAGQIGSELVPYLRKIYGGPNVVATYRRTPLIPEIM
                     EGGPCENMDALEAEKMFEVVKRYNIDTIINLVAVLSAVGENKPTLAWKINMDSMINSL
                     EVAKEMGAAVFLPSSIGAFGLSTPMDNTPQDTLMRPTTIYGISKVAAELLGDYYYVKY
                     GVDTRGLRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKSYKCNLSAGTFLDMMYMPDA
                     LRALVELMEADPAKLKHRNCFNVSAMSFDPEMIAAEIKKHIPEFHMEYEVDPVKQAIA
                     KTIAFNSGKGK"
     gene            complement(986..>2044)
                     /locus_tag="PLB85_06645"
     CDS             complement(986..>2044)
                     /locus_tag="PLB85_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOU73046.1"
                     /translation="EEVEQRLLELGEAPPEVKWDSNVEEYTRLAEYENMVNSLNPLVN
                     PKTGEKYNFENRGYQEKLDQCKFTAAEFHYQKALDFLEQGDNPDIHRNAVKELQATLN
                     YVPDYRDALQLYTHSRQFSTKRVAISPFEDKSGTRDKYGGLIDLLTDTLISKLVQDKT
                     VSEYWDIVSRDQINIVLTEQQYGNSQIPNSGNDFSRLQGAHEIMIGKIIQVNYVPERT
                     SELELRETKNIVTGKETYITDKGKKRQRKVKEDVTCVYRRYTKTASVRITASFTLVDV
                     KTGVAKTGDTVTAEYPWTDVWGRVVNGGDERVLSAEALALIRREEPFPPAEMDMVNVA
                     LNKLSDEIVARVRTYVGR"
BASE COUNT          370 a          531 c          556 g          587 t
ORIGIN      
        1 ccttgccctt cccggagtta aacgcgatcg ttttggcgat ggcttgtttc acggggtcga
       61 cttcatattc catgtggaat tcgggaatgt gtttcttgat ttcggcggcg atcatttcgg
      121 gatcgaagct catggcggag acgttgaagc agttgcggtg cttcagtttg gcgggatcgg
      181 cttccatcag ctccaccagg gcgcgcaggg cgtccggcat gtacatcatg tccaaaaaag
      241 tcccggcgct cagattgcac ttgtagcttt tgtttttgat cgcttcgtaa tagatttcca
      301 cggcgtagtc ggtggttccg ccgccgggca gagtcacgtt cgagattatg cccggatagc
      361 gtaggccgcg ggtatccaca ccgtatttaa catagtaata gtcgcccaga agctctgccg
      421 ccaccttgga gatgccgtaa atagtggtgg ggcgcatcag cgtgtcttgg ggcgtgttgt
      481 ccatcggggt gctgagcccg aaagcgccta tcgaggaggg taaaaacaca gcagcgccca
      541 tttccttggc aacttccagc gagtttatca tgctatccat gtttattttc caggccaggg
      601 tcggtttgtt ttcacccacg gcggacagca ctgccaccag gttgatgatg gtgtcgatgt
      661 tgtatctttt caccacttcg aacatctttt ccgcttccag ggcgtccatg ttttcgcagg
      721 ggccgccttc catgatttca gggatgaggg gggttctgcg gtaggtagcc acgacgttcg
      781 ggcctccgta gatctttctc aggtagggaa ccagttccga tcctatctga ccggctgctc
      841 cgaggacaag gatgtttttc atttacgtct ccatcagttt tattctattt gaaactctct
      901 tttttgttcc ggctggattt gtcaatgctt ttcgggcttg ccgccggctc gaagggtccg
      961 cttgaacgtc tggcattctg aatgcctatc ggcccacgta agttcgcacc ctggccacga
     1021 tttcgtcgct gagcttgttc agggcaacat tgaccatgtc catttctgcg gggggaaaag
     1081 gctcttccct gcggatgagg gccagcgctt cggctgaaag cacccgctcg tcgccaccat
     1141 tcacaaccct gccccaaacg tccgtccagg gatattcagc ggtcacagtg tcgccggttt
     1201 tggccacacc ggttttcacg tcaaccaggg tgaaagaggc agtaatgcgc acggaggcgg
     1261 tcttggtata tctcctgtaa acgcaggtca catcttcttt cacctttcgc tggcgcttct
     1321 tgcctttgtc cgtgatgtat gtttcctttc cggttacaat gttcttggtt tccctcagct
     1381 ccagttcgct ggttctttcg ggcacgtaat taacctggat gattttgcct atcatgattt
     1441 catgcgcgcc ctgcagccgg ctaaaatcgt ttccggaatt cgggatttgg ctgttgccgt
     1501 actgctgttc ggtgagtacg atatttatct ggtcgcggct cacgatgtcc cagtattcgc
     1561 tcacagtctt gtcctgcacc agcttggaaa tcagggtgtc ggtgagcagg tcgatcagac
     1621 cgccgtattt atcccgggtg cctgatttgt cttcaaaagg ggaaatggcc acccgtttgg
     1681 tggaaaactg ccgggagtgt gtgtaaagct ggagcgcgtc gcggtaatcg ggaacgtagt
     1741 tcagagtggc ctgaagctct ttaacagcat ttctgtgaat gtcggggttg tcgccttgct
     1801 caagaaaatc cagggctttt tgatagtgga attccgctgc ggtgaacttg cattggtcga
     1861 gcttttcctg gtaaccgcgg ttctcaaaat tgtatttctc gcctgttttg gggttcacca
     1921 acggattaag gctgtttacc atattttcat attcggccag ccgcgtgtat tcctccacgt
     1981 tgctgtccca ttttacctca ggaggagctt cgcccagttc cagcagccgc tgttcgactt
     2041 cttc
//
LOCUS       DAOOWR010000290         2043 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_54909_length_2043_cov_3.843058, whole genome shotgun sequence.
ACCESSION   DAOOWR010000290 DAOOWR010000000
VERSION     DAOOWR010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54909_length_2043_cov_3.843058"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLB85_06650"
     CDS             complement(<1..945)
                     /locus_tag="PLB85_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU73047.1"
                     /translation="MENIRIGIVGNIGVGKSTFIEAASSAPLNKVLTSVYPKPNGTEG
                     VFAFPEKFNPLVLDAFYKDPVANAFMAQIEFFNGRLDRQKLIHACRGIVLEDRTLAED
                     YHIFGKAQRILKNMSEPEFMAYQRTYRLMTEQIKEPDLLVYFRAEVPVLLERIRERGR
                     ESELAISADYLQLLNNLYEDFVANHVKCPVLVINADKSVEKMEWQRRTANLIAEQVKS
                     LKLRVSSPGISEWVKLPQTEATLRAIDVERQLEEYLAEHPKLITIAGNVGLGKTTLAA
                     IMERSLKINTLYEKPEENPLLEKFLGDKKAHCYALQKHF"
     gene            complement(1252..>2043)
                     /locus_tag="PLB85_06655"
     CDS             complement(1252..>2043)
                     /locus_tag="PLB85_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73048.1"
                     /translation="AEFESQGLSNKIMDWLQIPSGMQNTLLQQYQALASKISSVWNQL
                     YDSLDNRKEQALEALEKRAKTEYRSEAWLAAEKAKIEEEFAEKQKTLKKTEQKMQIAS
                     ATANTAEGVTNALTVKPAILGTALAALHLGLGLAQVAIIADQKFSHGGHFRGKGTTTS
                     DSNLIAVSDQEYIISAERVARFGVPFFNALNFGSGEQIRKALASIKIPNTMTVYPQKA
                     RGFASGGVVSPQASAQNVSMNVVLKCDGKALAKAVIKGKKKIIST"
BASE COUNT          409 a          501 c          551 g          582 t
ORIGIN      
        1 aaaatgcttc tgaagagcat agcaatgggc tttcttgtca ccaagaaact tttccagcag
       61 cggattttcc tccggctttt cgtaaagcgt gttgattttc aggctgcgtt ccatgatggc
      121 cgcgagggtg gttttgccca gaccgacgtt gccggcgatg gttatcagct tgggatgctc
      181 ggccagatat tcttccagtt ggcgctcgac gtctattgcc ctgagggtgg cttctgtttg
      241 gggcagtttc acccattcgc ttattccggg gctcgaaacg cgaagcttca ggcttttcac
      301 ttgttccgcg ataaggtttg cggtgcggcg ctgccactcc atcttttcca ctgacttgtc
      361 cgcgttgatc accaacacgg ggcacttgac atggttggct acgaaatcct catacaggtt
      421 gttcaaaagc tggaggtagt cggcggaaat ggccagttcg ctttcccggc cccgctccct
      481 gatcctttcc aaaaggaccg gaacttccgc cctgaaatac accagcaggt caggctcctt
      541 gatctgttct gtcatcagcc ggtaggtgcg ctggtaggcc atgaattcag gttcggacat
      601 gtttttgagg atgcgctggg ccttcccgaa aatgtgataa tcttccgcca gggtcctgtc
      661 ttccagcacg attcccctgc aggcatggat cagcttctgg cggtccagcc ggccgttgaa
      721 gaactcaatt tgggccatga aggcgttggc gacggggtct ttgtaaaaag catccagcac
      781 gaggggattg aatttctccg gaaaggcgaa aaccccctcc gtgccgttgg gctttggata
      841 aacggaagtg agcaccttgt tgaggggcgc ggaactggcc gcttcaatga aagtggattt
      901 gcccactccg atgtttccga caatgccaat tcggatgttc tccattaaag atctcccaaa
      961 taaaaattag tgcatccctt accattcacg cccgtttgtt ttgctgtaat tatatgaagc
     1021 cgtttcatcc tgtaaacatg catctgactg cccaaatatt cagcggagcg gtaacagtca
     1081 agacattatt tcaaggtcat cataattatg gttggagggc gcaaatatcc cccgacaaac
     1141 cctttggtca ctttcgacaa tccctttgaa aatctcggaa aatgacaagc ggtttggaaa
     1201 agaaagggct atatttatag acgaaatgac aaacggtttg gaaattgtcc gttaggtgga
     1261 gatgatcttt ttcttgcctt ttatcaccgc ttttgcgagg gctttcccgt cacatttcag
     1321 gacaacgttc atagacacgt tttgggcgga tgcttgggga ctcacgaccc cgccagaggc
     1381 aaagcctcgt gctttttgtg gatagactgt cattgtgttt ggtattttta tgctcgccaa
     1441 tgctttccgt atctgctcgc cgctcccgaa gtttagggca ttgaagaagg ggactccgaa
     1501 acgagcaaca cgttcagcgg agatgatgta ttcctggtcg gataccgcta tcaggttgga
     1561 atcgctggtg gtggttcctt ttcccctgaa gtgtcctcca tgtgagaatt tctggtcggc
     1621 gatgattgcc acctgagcca gtcccagtcc caggtgcagc gcggccaaag cggttccgag
     1681 gatagctggt ttgacggtca gcgcgttggt cacgccttcc gcggtatttg cggttgcgga
     1741 ggcgatttgc attttctgtt cggtcttttt gagggttttt tgtttttcgg cgaattcttc
     1801 ttcgattttg gccttttcag cggccagcca ggcttcggag cggtattcgg tttttgctcg
     1861 tttttccagc gcttcgaggg cttgttcctt ccggttgtcc aggctgtcgt aaagttggtt
     1921 ccaaacgctg gagattttgc tggcgagtgc ctggtattgt tgcaggaggg tgttttgcat
     1981 cccggaggga atctgcagcc agtccatgat cttattggac aggccctggg attcgaattc
     2041 cgc
//
LOCUS       DAOOWR010000291         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_55224_length_2035_cov_3.449495, whole genome shotgun sequence.
ACCESSION   DAOOWR010000291 DAOOWR010000000
VERSION     DAOOWR010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55224_length_2035_cov_3.449495"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLB85_06660"
     CDS             <1..183
                     /locus_tag="PLB85_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73049.1"
                     /translation="LPPSCHNFLYTDILFYQLLCFTLKQPPQGAWGCFGSTPSSGFAQ
                     VFTEGLEDILPCGTIC"
     gene            403..>2035
                     /locus_tag="PLB85_06665"
     CDS             403..>2035
                     /locus_tag="PLB85_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU73050.1"
                     /translation="MNPPFDFTLKSMIDRSCELYNRRPALSNVDGEPITYGEMRKHID
                     GLVRMMKEQGIVKGDRVALLGQNMPNWGIAYLAITSMGAVVVPILTDFHVNEILNILR
                     HSEARMVFVSGAHYDKIGYADLDPRITLVALDNFELLEHKAPKDLIGGLIYEPLKQLQ
                     KLSSRALKAVGLFDGKVHEDDLAALIYTSGTTGFSKGVMLSHKNLVLDAWITTQFQPV
                     KVTDRLLSVLPLSHTYECTVGFIIPMMCGACIYYLDKPPTARVLVPAMQKIKPTMILT
                     VPLIIEKIFKMQVHPQLTANPLFRNLYKMPPTRKLLHKLAGKKLMKTFGGQLHFFGIG
                     GALLSWEVERFLNEAGFPYAIGYGLTETAPLIAGSNPQSTRFRSTGHVLEHLQVRIDN
                     PDPKTRIGEILVKGDSVMKGYYKDPQRTAEAFTEDGFFRTGDLGILRKNRYLYIIGRT
                     KNVIVAASGENIYPEEVKNKLNEHEAVLESLVLEVGGQLTARVFLNPEYLEKHYHPDK
                     TGSVNYQRNLNLLLEEIKTAANLNLSAFSRIKKIVQ"
BASE COUNT          506 a          588 c          501 g          440 t
ORIGIN      
        1 ctaccaccgt cctgccacaa ttttctatat acagatatcc tcttttatca gttattgtgt
       61 tttaccctga aacaacctcc acaaggcgca tggggatgtt ttgggtcaac gcccagctca
      121 ggttttgctc aagttttcac ggaaggatta gaagatattc ttccttgcgg cacaatctgt
      181 taaccaattg tatgtcaggc tggtgccact tccttagccg tcttttcttc accccttgcc
      241 agcctcccgt attattcccc catgccatgc gggtattatt cgggaggcaa cttataggca
      301 aaatggacag ggctaaggac gcatgcggaa aaacttagct tgacaggaaa tcccggtatt
      361 ctggaagctg ctgagattat gatgaaccca ctggaagaaa aagtgaatcc tccttttgac
      421 tttaccctga aaagcatgat cgacaggtcc tgcgaactat acaaccgcag gccggcatta
      481 tcaaacgtgg atggcgaacc cataacttat ggcgaaatgc gcaagcatat cgatggcctg
      541 gtgcgcatga tgaaagagca aggcatcgtg aaaggcgaca gggtggcgct tttgggccag
      601 aatatgccga actggggcat tgcctatctg gccattacct ccatgggcgc tgtggtggtg
      661 cccatcctca cggattttca cgtgaacgaa atcctgaaca ttctgcgcca ctcagaggcc
      721 aggatggtct tcgtttccgg cgcccattac gacaaaatcg gctacgcgga cctggacccc
      781 aggatcaccc tggtggccct cgacaacttt gaactcttgg aacacaaagc tcccaaagac
      841 ctgatcggcg gccttatcta cgagccactt aaacagttgc agaagctaag cagccgcgcc
      901 ctcaaggcag tggggctgtt cgacggcaaa gtccacgagg acgaccttgc cgcgctgatc
      961 tatacttccg gcaccaccgg gttttccaag ggcgtgatgc tgagccacaa aaacctggtt
     1021 ctggatgcct ggatcaccac ccagttccag ccggtgaagg taacggaccg cctgctttcg
     1081 gttttgccgc tctcccacac ctatgaatgc acggtggggt tcatcattcc gatgatgtgc
     1141 ggcgcctgca tttactatct ggacaaaccg cccacggcgc gggtgctggt cccggccatg
     1201 caaaagatca agcccaccat gatcctcaca gtgccgctga tcattgaaaa gatattcaag
     1261 atgcaggtgc atccgcaact gacagccaat ccgctgttcc gcaacctcta caaaatgccg
     1321 cctacccgga agctgctgca caagctcgcc ggcaagaaac tgatgaagac attcggcggc
     1381 cagttgcatt tcttcggcat cggcggagcg ctgctatcct gggaagtgga acgctttctg
     1441 aacgaagccg gcttccccta cgccatcggc tatgggctaa cggaaacggc gccgctgatc
     1501 gccggcagca atccccagtc cacgcgcttc cgttccaccg gccatgtatt ggagcatctg
     1561 caggtgcgca ttgacaatcc cgatcccaaa acccgcatcg gcgaaatcct tgtgaagggt
     1621 gattcggtga tgaaaggtta ttacaaagac cctcagcgca cagcggaagc cttcacggaa
     1681 gacggtttct tccgcaccgg cgaccttggc atcctgcgca aaaaccgcta cctctatatc
     1741 atcggccgca ctaaaaacgt gatcgtagcc gcctccggag aaaacatcta tcccgaggag
     1801 gtgaaaaaca agctgaatga acacgaagcc gtcctggaat ccctggtgct ggaagtgggc
     1861 ggacagctca ccgcgcgtgt cttcctgaac cccgaatacc tggaaaaaca ctaccatccg
     1921 gacaaaacag gcagcgttaa ctatcagaga aatctgaacc ttctcctgga agagatcaag
     1981 acggctgcca acctcaacct ctccgccttt tcgcgaatta aaaagatagt ccaac
//
LOCUS       DAOOWR010000292         2034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_55257_length_2034_cov_5.033855, whole genome shotgun sequence.
ACCESSION   DAOOWR010000292 DAOOWR010000000
VERSION     DAOOWR010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55257_length_2034_cov_5.033855"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLB85_06670"
     CDS             complement(<1..601)
                     /locus_tag="PLB85_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOU73051.1"
                     /translation="MKYLDLAMNAAERLGAEYADIRVQKTTDEVIYLQNLSLKNTSNE
                     VLYGYGIRFFRDGAWGFAHSNVFSDEAVLKTVSRAAEIAALSASVNKDKKLRLAPERS
                     YIAKYETPVKIDPRDVPMKEKVELMLEVNRTMLAFEGVKRAMFYLVMHRDEKFFGSTL
                     GTRLELNTQWITPMITATAVYEGDSQSRSFNEGGKATGWE"
     gene            796..1155
                     /locus_tag="PLB85_06675"
     CDS             796..1155
                     /locus_tag="PLB85_06675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOU73052.1"
                     /translation="MECPFCAIKPEQVLLENEHFVAIRDIRPISPGHCLIVSRRHARD
                     FFELSAAEAASLHGLSLKLREFLEQEHHPDGFKLLMNCGAAAGQSVFHFHLHLIPRYT
                     GDRKNFRRLVASLGEVL"
     gene            complement(1846..>2034)
                     /locus_tag="PLB85_06680"
     CDS             complement(1846..>2034)
                     /locus_tag="PLB85_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73053.1"
                     /translation="NNHHPTPNPRHSGLDPESSPRGMTVKRNKVYPPSMNLSCFFPKM
                     KPVFLLPAEKACQNTRAG"
BASE COUNT          428 a          596 c          521 g          489 t
ORIGIN      
        1 attcccagcc ggtggctttg ccgccttcgt tgaagctgcg gctttggctg tcgccttcgt
       61 aaaccgccgt ggcggttatc attggcgtta tccactgcgt gttcagctcc aggcgggtgc
      121 ccagagtgga gccaaagaac ttttcatccc tgtgcatcac caggtagaac atcgcgcgtt
      181 tcacgccttc aaaggccagc atggtgcggt tcacctccag catcagctcc accttttcct
      241 tcatcggcac gtcgcgggga tcgatcttca ccggggtctc gtatttggcg atatagctgc
      301 gttccggggc caggcgcagc ttcttgtcct tgttcaccga ggctgagagg gcggcgattt
      361 ccgcggcgcg gctcaccgtc ttcaaaacag cctcatcgct gaaaacgttg gaatgggcga
      421 agccccaggc cccatccctg aaaaagcgga tcccgtagcc gtaaagaact tcgttggacg
      481 tgtttttaag gctcaggttc tgcagataga tcacctcatc ggtggtcttc tgaacgcgga
      541 tgtcggcata ttcagcgccg agccgctccg ccgcgttcat ggccagatcg agatacttca
      601 tcaatcctcc caatttggat tttggcttgg ttgtgccgcc ttcagactgt gcaacctgcc
      661 ttccaatcca gtataaaatc cccgcccccg cctgtcaagc ctgatttttc ctttgacaga
      721 acggcccgcc tccgccaagc tatcccggtc ggccaaccgg ctgaaaacat tacggttaac
      781 aaggacaaac gcgatatgga atgccccttc tgcgccatca aacccgaaca ggtcctgctg
      841 gaaaacgaac attttgtcgc catccgcgac attcgcccca tctcgccagg acattgcctc
      901 atcgtttcgc gccgccacgc tcgcgatttc tttgagctta gcgccgccga agccgcttcc
      961 ctgcacggtc tcagccttaa gctgcgcgag tttctggagc aggaacacca tcccgacgga
     1021 tttaagctgc tgatgaattg cggcgccgcc gccggacaga gcgtgttcca tttccacctg
     1081 catctgatcc cgcgctacac cggcgacagg aagaatttcc gcaggctggt ggcctcgctg
     1141 ggggaagtcc tgtaaccctg aatccaccga tatactgagc ccgaaatgtt gggataaaac
     1201 ccaacccatt gctcctctgg ctgtttgagg gcgtctgaca ttccccgtta ttcatggact
     1261 atgcaaggcc aggcgggcaa gcgcattgtt ttgatcggct ctttttcaaa ccgggcaggt
     1321 aagcccggag ggcggcaaag acaaattcac ggatttattc atctgcgaaa tccgtgaaat
     1381 ctgtgtgaaa ctcactttta ttcgcgtgat tcaagaaccc cctgttttcc agggaaatgc
     1441 gacggacgcg ggtcccggaa tccggattct ccgcgtccaa tcctggctca acccctcccg
     1501 gcgagggagg tcatttcaag aaccatcatc atccacaact actacaagta ccaatcgtct
     1561 caacccctcc cggcaaggga ggtcatttca agagagcctc gattttagcc ctttagcaga
     1621 gcgggtttgg gaccccattt ccgtgaacct cctctgaccc gggcctgaaa tgaaggtcga
     1681 aaaggcaggt tcgcggaaaa caggctcaaa gcctctccac aagcggcttc acggctattg
     1741 acatatcata tctgtatgtc aggcatacag ataatagcgt tttgtgcctc gtgaaaggct
     1801 cttgccagga cagagtccgt aactttatca aggctgtttc cactttcagc cagcccgcgt
     1861 attttggcaa gctttttctg cgggcaataa aaacaccggt ttcattttcg ggaaaaaaca
     1921 gctcaagttc attgatggcg gatagacttt attccgtttt accgtcatgc cgcgcgggct
     1981 ggattccggg tcaagcccgg aatgacgtgg gtttggggtt gggtggtggt tgtt
//
LOCUS       DAOOWR010000293         1993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_56807_length_1993_cov_3.979876, whole genome shotgun sequence.
ACCESSION   DAOOWR010000293 DAOOWR010000000
VERSION     DAOOWR010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56807_length_1993_cov_3.979876"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PLB85_06685"
     CDS             <1..1357
                     /locus_tag="PLB85_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73054.1"
                     /translation="DLLFDGEGKLRRKGWETWQDVAKKIFFLRFSDRRDSLYFKIGCI
                     PDYTLGHGLIFDDYSNMLRYPEEKPIGGYLGANTNAYGFGFEVYTHDISKNEVVAGRA
                     SIKPLLGTRLPLLKNLTLGVNLGADRDPYGKYPDTDGDGYPDVYDKFPLDPDSWLDTD
                     DDGVPDQIDIDLNGNSVLDHPSLNPYVNEVFPNIGQLYPDYPFDTAIFPDSAACYPDP
                     RPMWVYSVDYELPIVDNENFRLSNYGEYAVMKDYGSGLILPGLAARFLIFDVKLEFRS
                     FTDCFLPAYFNHLYDEQRCQVIYTGIEGQEGRRYYSLRTKDEDLTGLESSLGWFGYLK
                     GNIANFAQLKVAYQDKFNAEVAKGKSLWAKLSFMPEKFPKLREASLYYAQTDVDRLDL
                     KNWKNTSAELNGLIVYGYNESYNLICKYREFYQDANGDGIIAGQDEIIESLSLGIEFQ
                     F"
     gene            complement(1348..>1993)
                     /locus_tag="PLB85_06690"
     CDS             complement(1348..>1993)
                     /locus_tag="PLB85_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73055.1"
                     /translation="ILELLSLREKRLEDALTALKSSLREDSDLDPDIRDCCRLLLEYY
                     PKDAGVFAPLYLNLFTLQPGEALFLATGVPHAYLRGAGIEIMGNSDNVLRGGLTPKHV
                     DVEELARVLDFEPYSGGVVTLQADGPFSGSYPSPVQEFELRRIDLGGGWGQGYDVGRA
                     PLIVFCADGSAILESRTSRLELVKGEAAFVSADVESLHFAGDGTLWLATLPAQN"
BASE COUNT          486 a          578 c          500 g          429 t
ORIGIN      
        1 cgacctgctc ttcgacggtg agggaaagtt gcgccgcaag ggctgggaaa cctggcagga
       61 tgtggccaaa aagattttct tcctccgttt ttccgaccgc cgcgactctc tttacttcaa
      121 gatcggctgc attccggact acactctggg gcacggcctg attttcgacg actattcaaa
      181 catgcttcgc tatcctgagg aaaaacccat cggcggctat ctgggcgcta acaccaatgc
      241 ctacggcttc ggttttgagg tttacacgca tgacatcagc aaaaacgaag tggtagccgg
      301 acgcgcctcc atcaaaccct tgcttgggac cagactgccc ctgctcaaaa acctgaccct
      361 cggcgtgaac ctcggcgctg accgtgatcc ttatggcaag tatccagata ccgacgggga
      421 cggttatccg gacgtttatg acaaattccc gctcgatccc gacagttggc tggataccga
      481 tgacgacggg gtgccggacc agatcgacat tgatttgaac ggcaactccg ttctggacca
      541 tcccagcctc aatccctatg tgaacgaggt tttccccaat attgggcagc tatatccaga
      601 ttatcccttc gacacagcca tttttccgga ttccgcggcc tgctaccccg atcccaggcc
      661 gatgtgggtt tacagcgtgg attatgaact gccgatagtg gacaatgaaa atttccgcct
      721 cagcaattac ggcgaatacg ctgtgatgaa ggattatggc agcggcctca tcctgccggg
      781 actggccgcg agattcctga tcttcgatgt caagctggaa ttccgcagtt tcaccgattg
      841 cttcctgcca gcctatttca accatctcta cgacgaacaa cgctgccaag tgatctatac
      901 tgggatagaa ggccaggaag gccgccgcta ttacagtctg cgcaccaagg acgaggacct
      961 gacgggattg gaatccagcc tgggctggtt cggctatctc aaaggcaaca ttgccaattt
     1021 cgcccagttg aaagtggcct atcaggataa gttcaacgcg gaggtcgcca agggaaaatc
     1081 actctgggcc aagctttcct ttatgcctga gaagtttccc aaacttcgcg aagcctcgct
     1141 ctactatgcc caaacggatg tggacaggct ggacctgaaa aactggaaga ataccagtgc
     1201 cgagcttaac ggcctgatag tttacggcta caacgaatcc tacaacctga tctgcaaata
     1261 cagggagttt taccaggacg ccaacggcga cggcatcatc gccggacagg acgagatcat
     1321 cgaatcactg agcctgggaa ttgaatttca gttctgagct ggcagtgtgg ccagccaaag
     1381 cgtgccgtct ccagcgaaat gcagactctc tacatctgca gaaacaaagg ccgcttcgcc
     1441 tttgactaat tccagtctac tagttcgact ctccagtata gctgaaccat ccgcgcagaa
     1501 aacgattagc ggtgcccgcc ccacatcgta gccctggccc cagccgccgc caagatcaat
     1561 tctcctaagt tcaaactcct gcaccgggct gggatagctg cctgaaaaag gtccatcggc
     1621 ttgcagggta accacgccac cggaataggg ctcaaaatcc agaacgcggg ccagttcctc
     1681 cacatcaacg tgttttggag tgaggccgcc acgcagcaca ttgtccgaat ttcccatgat
     1741 ctcgatgcca gcgccacgca gataggcatg aggtaccccg gtagccagga acagcgcttc
     1801 gccgggttgc aaggtgaaca ggttcagata cagcggagca aaaaccccgg catccttggg
     1861 ataatattcc agcaacagcc tgcagcagtc ccggatgtct ggatcaaggt cggaatcttc
     1921 acgcaaagag cttttcagcg ccgtcaacgc atcttccagc cttttttcgc gcaagctcaa
     1981 aagctccagg atc
//
LOCUS       DAOOWR010000294         1989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_56967_length_1989_cov_3.718201, whole genome shotgun sequence.
ACCESSION   DAOOWR010000294 DAOOWR010000000
VERSION     DAOOWR010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56967_length_1989_cov_3.718201"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLB85_06695"
     CDS             complement(<1..342)
                     /locus_tag="PLB85_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU73056.1"
                     /translation="MTVAIFVGIVIALFVIILISRSIIVVRQAEVVIVERLGKYYKTL
                     TSGIHIIVPIFDKIRPIHWRYNRADYRGNVIVTQRQENRIDLRETVYDFPRQNVITSD
                     NVSININALLYL"
     gene            554..1063
                     /locus_tag="PLB85_06700"
     CDS             554..1063
                     /locus_tag="PLB85_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73057.1"
                     /translation="MKKVLVLMLCTLALWGCAQRRMDGWNEEDVSLLTRELAADITGS
                     AWLREHIGAKGQAPALLIRPLERRGNCQSCLSGLESALARELLLSGKIRLVRFRRGES
                     SSADSIGLLLQDLSLQESGADALLTWWLEEIPEPKLLNLRITLQLLDPNSQELLLQAQ
                     RTRSKQLWL"
     gene            1116..>1989
                     /locus_tag="PLB85_06705"
     CDS             1116..>1989
                     /locus_tag="PLB85_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOU73058.1"
                     /translation="MQITKLDQMIEVLKPFPNKRLVAAWAVDAHTICAVHQAVEMDLV
                     EGILVGDEKLILKVCEAEKIDPTVFKIVHSPSDTAAAAKAVDLINSGKGDFLMKGLLS
                     TDRYMKAILNKERGLMENGAILSHVTVAEPKSYHKLLIFGDVAIIPLPDLKQKVAITN
                     YLIRVAHFLGIEKPKVGIQAASEQTLPKIPSCADGALIAKMAERGQIKGAIVEGPLGF
                     DLIVDKESALIKGVQSEVCGDADCILFPNIEAGNCFYKSIVKLMDSELCAVVMGAKVP
                     CVLTSRGDSERSKLY"
BASE COUNT          494 a          540 c          575 g          380 t
ORIGIN      
        1 taaatagagg agggcgttga tgttgatgga aacgttgtcg ctggtgatca cgttctggcg
       61 gggaaagtca taaacggttt cacggaggtc gatgcggttt tcctgacgct gggtgacgat
      121 cacgttgccg cggtagtcag ccctgttgta gcgccagtgg atggggcgta tcttgtcgaa
      181 aatgggcacg atgatatgga ttccagaggt cagggtcttg tagtatttgc ccagacgctc
      241 cacgatcacg acctcggcct gacgcacaac tatgatgctg cgggagatca gaattatcac
      301 aaagagggcg ataacgatcc caacaaagat ggcaactgtc atgattcact ccttaaggtt
      361 agaatttagt tcactttatc tgcggcccat attctgacaa ggaaaaaatc accaagccgg
      421 acacggaggg aaaattggac ttgacaaata tcaaaacttc cggcgaggat gattttttct
      481 taacaaagcg ggagtctggg ccggacatcc cggttcactc gcagatatat tagtccaaag
      541 cagggagata acaatgaaaa aagttctggt cctgatgcta tgcacacttg ccctctgggg
      601 ctgcgcccag cggcgtatgg acggctggaa cgaagaggat gtttccctgc tgacccggga
      661 actggcggct gatatcaccg gcagcgcctg gctacgcgaa cacattgggg caaaaggaca
      721 ggcgcccgcg ctgctgatcc ggccgctgga acgcaggggc aactgccaaa gctgcctgag
      781 cggcctggaa agcgctctgg cacgggaatt gctgctgagc ggcaaaatcc ggctggtgcg
      841 gttccggaga ggggaaagca gctccgcgga cagcatcggt ctcctgctgc aagacctttc
      901 gctccaggaa agcggggccg acgccctgct gacctggtgg ctggaagaaa taccggaacc
      961 gaagctgctc aacctccgga tcacgctgca actgctggac ccaaacagcc aggaacttct
     1021 gctgcaggcc cagcgaacgc gcagcaagca attgtggctg tgaagcccgg ttcgccaaat
     1081 tgaatcgcaa gaaataaaac atagatggag tattaatgca gatcaccaaa ctggaccaga
     1141 tgatcgaagt cctgaaacct tttccgaaca agcgcctcgt ggctgcctgg gcagtggacg
     1201 cccacaccat ctgcgccgtg catcaggccg tggaaatgga cctggtggaa ggcatcctag
     1261 tgggcgatga aaagctcatc ctcaaggtgt gcgaggctga aaagatcgat cccacggtgt
     1321 tcaaaatcgt gcactcgccc tcagacacag ccgcggccgc caaagccgtg gacttgatca
     1381 attccggcaa aggggatttc ctgatgaagg gcctgctgag caccgaccgc tatatgaaag
     1441 caatcctgaa caaggaacgc ggcctgatgg aaaacggcgc catcctttcc cacgtaaccg
     1501 tggcggaacc caaaagctac cacaaactgc tcatcttcgg cgacgtggcg attatcccgc
     1561 tgcccgatct gaagcagaaa gtagccatca cgaactatct catccgcgtg gcgcacttcc
     1621 tgggcatcga gaaacccaag gtgggcatcc aggccgcctc tgaacaaacc ctgcccaaaa
     1681 tcccctcctg cgccgacggc gccctcatcg ccaagatggc agagcgcggg cagatcaaag
     1741 gcgcgattgt ggaagggcct ctgggcttcg acctcatcgt ggataaggaa agcgcgctga
     1801 taaagggcgt gcagagtgaa gtttgcggag acgccgactg catcctcttc ccgaacatcg
     1861 aggccgggaa ctgcttctat aaatccattg tcaaactgat ggacagcgag ctctgcgccg
     1921 tggtgatggg cgccaaggtg ccctgcgtgc tcacttcgcg gggcgacagc gaacgctcca
     1981 aactctatt
//
LOCUS       DAOOWR010000295         1949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_58633_length_1949_cov_4.268743, whole genome shotgun sequence.
ACCESSION   DAOOWR010000295 DAOOWR010000000
VERSION     DAOOWR010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58633_length_1949_cov_4.268743"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLB85_06710"
     CDS             <1..717
                     /locus_tag="PLB85_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU73059.1"
                     /translation="RLVAGYSLYSKKYEITNHSMIDPFTNATLVELDTLGWRYTSAVS
                     ATVSRDTRNNIYFPSRGTQMTLFSELAGGLLGGNFDYFKQIAQVNWYMELWYKLTLRT
                     KWRFGYITPYGSSTDAPPDEKFYLGGTGVDGIRGYPDRRIGVDEDGDGEMDTGGTREI
                     IFSTELGYPIGSDQIIGLVFFDAGDCYNKLRDFNFLKFKKGVGAGVRIQSPFGLIGFD
                     YAYNLDKGTWEPHLQFGTTF"
     gene            718..1665
                     /locus_tag="PLB85_06715"
     CDS             718..1665
                     /locus_tag="PLB85_06715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU73060.1"
                     /translation="MKYKHLFGPVHSRRLGYSLGVDLVPFKYCPLSCVYCEVQITDHL
                     TLERREFFPLEEITAELVDFLKTKPELDYITFSGAGEPTLYSRIGEIVRFVKGEYPEY
                     KLALLTNGTLLNDAALRQEILPCDLILPSLDACHQQTFRIINQPHPALTAEGLVEALT
                     LLREDYKGLIWLEVFIIAGVNDSEDEMACLCRAIDKIRPDLVQINSLDRPGAEAWVKA
                     AGDEVLTKVLNYFSARLDMPVEIIAKAQQEIAVPPVPEDIEATIAATLKRRPSTAEDR
                     SVTLGLHINEISKYLRQLNSEHKISVRREQRGIFYSWKQ"
     gene            1662..>1949
                     /locus_tag="PLB85_06720"
     CDS             1662..>1949
                     /locus_tag="PLB85_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU73061.1"
                     /translation="MTTAIVTAAGSGSRMGGKVRKQWLRLGGIPILIHTLQKFFNSAH
                     VDNIIVTAPEEELGYCEELIHEYFEDSVKPWLVVCGGIERQDSVFCALQNCP"
BASE COUNT          454 a          550 c          512 g          433 t
ORIGIN      
        1 aggctggtag cgggttattc actctattct aagaaatacg agatcaccaa ccatagcatg
       61 atcgatccct tcaccaatgc cactctggtg gaactggaca ccctgggctg gcgctatacc
      121 agcgctgtga gcgccaccgt aagccgggac acccgcaaca atatctattt cccttcccgc
      181 ggcacgcaga tgactctgtt ttcggaactt gccggcggct tgctgggagg aaacttcgat
      241 tatttcaaac aaattgccca ggtgaactgg tacatggaac tctggtataa gctgaccctg
      301 cgaaccaagt ggcgctttgg ttacatcacc ccctacggtt cttccacaga cgctccgcca
      361 gacgaaaaat tctatctggg aggcaccggg gttgacggca tccgcggcta ccccgaccgc
      421 cggatcgggg ttgacgagga cggggacgga gagatggata ctggcggaac ccgagaaatc
      481 atcttttcca ctgaactggg ctatcccatc ggtagcgacc agatcatcgg actggttttc
      541 ttcgacgccg gcgattgcta taataagctt cgcgatttca atttcctcaa gttcaagaag
      601 ggcgtggggg cgggtgtccg catccagagc ccctttggct tgatcggatt cgactatgcc
      661 tacaaccttg acaaaggcac ttgggagcct catctccagt ttggcactac cttctgaatg
      721 aaatacaaac atcttttcgg cccggtccat tcccgccggt tgggttattc cctcggcgtg
      781 gacctggttc ctttcaaata ctgtccgttg agctgcgtct attgcgaggt tcagataact
      841 gaccacctca ccttggaacg ccgggagttc tttcctctgg aagagatcac cgctgaactg
      901 gtggattttc tgaaaacaaa gccggaactg gattacatca ccttttccgg tgctggtgaa
      961 cccacgcttt acagccgcat cggcgaaatt gtgcgctttg ttaagggcga atatccagag
     1021 tacaaactgg ccctgctcac caacgggact ctgctgaacg atgccgccct gcggcaggaa
     1081 atcctgccct gcgacctcat cctgccatcc ctcgacgcct gccaccagca gactttcagg
     1141 ataatcaacc agccccatcc tgccctaacg gctgaagggt tggtggaagc cctaaccttg
     1201 cttcgggaag attacaaagg cctgatctgg ctggaggttt tcatcatcgc cggagtcaac
     1261 gattccgagg atgagatggc ctgcctttgc agagcgatag ataagattcg gccggatttg
     1321 gttcagatca attccctgga ccgccccggc gcggaagcct gggttaaagc ggcgggggat
     1381 gaagtcctga ccaaggtttt aaactatttc agcgcccggc tggacatgcc tgtggaaatc
     1441 atcgccaaag cgcagcagga aatagcggtt ccgcctgtgc cggaagatat tgaagcaaca
     1501 atcgctgcca cactcaagcg gcgtccttcc accgcggaag accgctccgt cactctggga
     1561 ctgcacatca acgaaatcag caagtatctg cgccagttga actctgagca taaaatcagc
     1621 gtcaggcgcg aacaaagggg aatcttttac tcatggaagc aatgaccaca gcgatagtta
     1681 ccgccgccgg ctcaggcagc cgcatgggtg gaaaagtgcg caaacaatgg ctgcgtctgg
     1741 gcggtattcc catcctcatt cacaccttgc aaaagttttt caactcagcc cacgtggaca
     1801 acattatcgt aaccgccccc gaagaggaac ttggctactg cgaagaattg attcacgaat
     1861 actttgagga ctccgtgaag ccctggctgg tggtttgcgg cggtatcgag aggcaggaca
     1921 gcgtgttctg cgccctgcaa aactgcccg
//
LOCUS       DAOOWR010000296         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60148_length_1914_cov_3.948359, whole genome shotgun sequence.
ACCESSION   DAOOWR010000296 DAOOWR010000000
VERSION     DAOOWR010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60148_length_1914_cov_3.948359"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLB85_06725"
     CDS             <1..852
                     /locus_tag="PLB85_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU73062.1"
                     /translation="VMQIQRAFVSLKRILELTSLKSEEELFTGTKLPSFEHEIRFENV
                     WFAYKNEDWVLKDVSFTIPKGQKIALVGASGSGKTTTVSLLCGFYPVNKGRIFIDGVP
                     LSEIEFRAWRRKIGLILQDIFLFPGSILENVRVYNDAIPEAKVREAINIVQLDEFIRG
                     QNLGLDSELAERGQNVSQGEKQLISFARALAFEAELIIMDEATASIDPHTEAKIQHSM
                     ETVLSNKTAVVVAHRLTSVLDADEILFFDGGEIIHRGKHQQLMQSSKEYRKLVELQLI
                     GTGAADE"
     gene            845..1855
                     /locus_tag="PLB85_06730"
     CDS             845..1855
                     /locus_tag="PLB85_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOU73063.1"
                     /translation="MNKHVRWIVNKWMTQKWFLLIMLLFTLGSSAVAIAHPIVFGRLI
                     DLLKGILASPDKYPEPMAEVNRIIWIMLGLGAAQLVTGFYPAVRGWVNIRFENMLRMF
                     YFKFILGKDFRFFQKFRTGDVVTRLTDDLTDYSKISWFMCSGIFRAVNSFSLIMFTLA
                     VMFSISPKLTLLSIAPLPLMMVVFYFTSDKLYRNFELNQQAISEINSQLEMSFSGIRI
                     VKAFVSEEKYNRFFDLALARRFKTEMGVVKLNAVLSLIYQYIDYFAQIGVIIFGGYMA
                     VKGRISVGTFYMFYTYLSMMIYPLLDLPKLFVSGKQAFVNIDRLEEMKNFPLQLRLDG
                     HC"
BASE COUNT          437 a          546 c          506 g          425 t
ORIGIN      
        1 gtgatgcaga tccagcgggc ttttgtgagc ttgaaacgca ttctggaact aaccagcctg
       61 aaatccgagg aagagctttt caccgggacc aagctgccct cattcgagca tgagattcgc
      121 ttcgaaaacg tctggttcgc ctataagaac gaggactggg tgctcaagga cgtttccttc
      181 accattccca aagggcagaa gatagcgctg gtgggcgctt ccggcagcgg caagaccacc
      241 actgtgagcc tgctctgcgg cttttatccc gtgaacaaag gccgcatctt catcgacggc
      301 gtgccgctga gcgaaataga attccgggcc tggcgccgca aaatcgggtt gatcctgcag
      361 gacatcttcc tctttcccgg cagtattctg gaaaacgtgc gcgtttacaa tgacgctatc
      421 ccggaagcca aagtgcgcga agccattaac atcgtgcagc tggacgaatt catccgcggg
      481 cagaacctcg gcctggattc cgagctggcg gaacgcgggc aaaacgtttc ccaaggagaa
      541 aaacagctta tcagctttgc ccgggccctg gctttcgagg cggaactgat catcatggac
      601 gaggccaccg cctccattga cccccatacc gaagccaaga tccagcacag catggaaacc
      661 gttttgtcga ataaaaccgc cgtggtggtg gcccaccgcc tcacctcggt tctggacgct
      721 gacgaaatcc tcttcttcga cggaggcgag atcatccacc gcggcaagca tcaacaactg
      781 atgcaaagct cgaaagaata ccgcaagctg gtggaactgc agttgatagg gacgggagcc
      841 gcggatgaat aagcacgttc gctggatcgt gaacaaatgg atgacccaga aatggtttct
      901 gctcatcatg ctgcttttca cgctgggcag ctccgcggtt gccattgccc atccgatcgt
      961 gtttggcagg ctgatcgacc tgctgaaggg catcctggct tcgccggaca aatatccgga
     1021 gcccatggca gaggtgaacc gcatcatctg gatcatgctg gggttgggcg cggcgcagct
     1081 tgtgaccggc ttttaccccg ctgtccgcgg ctgggtgaac atccgcttcg aaaacatgct
     1141 gcgtatgttc tatttcaagt tcatcctcgg caaggacttc cgcttcttcc agaaattccg
     1201 caccggggac gtggttacgc ggcttactga cgaccttacc gattactcca agataagctg
     1261 gttcatgtgt tccggcatct tccgggcggt taattccttt tctctcatca tgttcactct
     1321 ggcggtgatg ttcagcatca gccccaagct aaccctgctt tcgatagcgc cgctgccgct
     1381 gatgatggtg gtcttctatt tcacctcgga caagctctac cgcaatttcg aactgaacca
     1441 gcaggccatc agcgagatca acagccagtt ggagatgagc ttttccggca tccgcatcgt
     1501 gaaagccttt gtgagcgagg aaaaatacaa ccgcttcttc gacctcgccc tcgcccggcg
     1561 cttcaaaacc gagatgggcg tggtgaagct gaacgcggta ctgagcctga tctaccagta
     1621 catcgactac tttgcccaga tcggcgtcat aatcttcggc ggctacatgg ctgtgaaagg
     1681 caggatcagc gttggcacct tctacatgtt ctacacctac ctgagcatga tgatctatcc
     1741 gctgctcgat ttgccgaagc tcttcgtatc gggcaaacag gccttcgtga acatcgaccg
     1801 cctggaggaa atgaagaact ttccgcttca actccgactg gacgggcact gctaagccta
     1861 atgagatcaa ggaactgcgc ttcgacaagg tcacctgcac ctatccggaa aaaa
//
LOCUS       DAOOWR010000297         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60419_length_1908_cov_3.767404, whole genome shotgun sequence.
ACCESSION   DAOOWR010000297 DAOOWR010000000
VERSION     DAOOWR010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60419_length_1908_cov_3.767404"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLB85_06735"
     CDS             <1..853
                     /locus_tag="PLB85_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU73064.1"
                     /translation="DADGNLNADSWPLNQEQPDMRFHFPFCHWDTEETAGTVMMMFAF
                     NYIRLTGGGPDGSMACLWQDSYKARMYNLFPADSLQYAAYEDDPEIFLALSTDQGSHW
                     SEPVVLSGVENPELAGMTPMWVYPSDALLPLTTSDPTGDWKRLWLMFYDDYNWGSQLP
                     PEHGLEWGNINYMALDLQFPTVSTPQEEIPQVSSPTLNAYPNPFSAACVLNVALPAKS
                     QAELTVYNLRGQALRHLHNGVLPAGEAHFSWDGKDERGREVPSGIYLLRLDAGSTNAT
                     RRVARIK"
     gene            complement(929..>1908)
                     /locus_tag="PLB85_06740"
     CDS             complement(929..>1908)
                     /locus_tag="PLB85_06740"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU73065.1"
                     /translation="TASAADQAAIAAYQAAMPGYTVQGYYSSSWLSDDAAHCRVHTIF
                     DEQMIHIVHVQPTSATANSFVTLDVTITHSNPLNVGSTYIAYRHSTTGQWLTAPLTLV
                     SGNNWTASVPTPALGQTLYYYILATDTTGRTAQMPLCGASDPFELLVDTAGANTPPTI
                     ALPDTFEFNMNGSLMVDFSQYVDDVDGDTLTLSYSGNTNVNVAINGLTVTFTATADWY
                     GTEALTFTVSDGTDTASDGVNVKVNLAWLAVPDVSVSYIDTTTDYVKLQWSAITNANY
                     YQIWVSTDPYGTYTFLGETTDAYWNDMDLSHAMRFYKVIASNESYTNAK"
BASE COUNT          388 a          489 c          590 g          441 t
ORIGIN      
        1 ggacgcggac ggaaacctga atgctgacag ttggcccctg aaccaggagc agcctgacat
       61 gcgctttcat tttcccttct gccattggga cacggaggaa acggcaggaa cggtaatgat
      121 gatgtttgcc ttcaactata tccgcctcac cggcggaggc ccggatggca gcatggcctg
      181 cctctggcag gacagctaca aagcccggat gtacaatctg tttccggctg attctttgca
      241 gtatgcggct tacgaagacg atccggagat ttttctggca ctgagtactg accaaggctc
      301 gcattggagc gaaccggttg tgttgagcgg ggtggagaat ccagaactgg cgggcatgac
      361 accgatgtgg gtgtatccct ccgatgcctt gctgcctctg actacatcag atcctaccgg
      421 agattggaag cggctttggc tgatgtttta cgacgattac aactgggggt cgcaactgcc
      481 gcccgaacat ggattagaat ggggcaacat caactacatg gccctggatt tgcagttccc
      541 gactgtttcc acgcctcagg aggaaatacc gcaggtttcc agccccactc tgaacgcgta
      601 tccgaacccc ttttccgcag cctgtgtcct gaacgttgcc ctgcctgcaa aaagtcaagc
      661 ggagcttact gtttacaatc tgcggggcca ggcgctccgg catctgcaca acggcgttct
      721 tccagccgga gaagcccatt tcagttggga cggcaaggat gagcgaggcc gggaggttcc
      781 ctcagggatc tatttgttga ggctggatgc tggttccaca aatgccacgc ggcgggtggc
      841 gcggatcaaa tagcccccac ggattattat gaaaaaacaa acaagcccgg ctggaatggc
      901 cgggcttgtt ctttatggag ttgcttggtt atttggcgtt tgtataggac tcattcgaag
      961 cgatgacctt gtagaatcgc atcgcgtggc tgagatccat atcgttccag taagcatcag
     1021 tggtttctcc caggaaggtg taggttccat agggatcggt gcttacccag atctgatagt
     1081 agttcgcgtt ggttatggca ctccactgca attttacata gtcggtggtg gtatcaatat
     1141 agctgacact gacgtctggc accgccagcc aggcgaggtt gactttcacg ttcacgccat
     1201 cgctggctgt gtcagtcccg tcgctgaccg taaaggtcaa ggcttcggtg ccgtaccagt
     1261 ctgcggtggc ggtgaaggtg acggtcaggc cgttgatggc aacgttcaca tttgtgttac
     1321 cgctgtaact gagggtcagg gtatcgccat ccacgtcatc cacatactgg ctgaaatcca
     1381 ccatcaggct gccgttcatg ttgaactcga acgtatctgg cagagcgatg gtgggtggtg
     1441 tgtttgctcc agcggtgtcc accaacagct cgaagggatc gctggcgccg cagagtggca
     1501 tctgggctgt cctgcccgtg gtgtccgtgg ccaggatgta gtagtagagc gtctggccca
     1561 gggcgggggt aggcacgctg gcagtccagt tgttaccgga gaccagggtg agtggtgcag
     1621 tcagccattg tcctgtggtg ctgtggcggt aagcaatgta ggtgctgccg acattcagcg
     1681 gattgctgtg ggtgatcgtc acgtccaggg tcacaaagct gttggcggtc gccgaagtcg
     1741 gctgcacatg cacaatgtgg atcatctgct cgtcgaagat ggtatgcacg cggcagtgag
     1801 cggcgtcatc ggagagccag gaagagctgt agtagccctg cacggtgtaa cccggcatgg
     1861 ctgcctgata ggcggcgatg gcagcctggt cagccgctga agcggtcg
//
LOCUS       DAOOWR010000298         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60655_length_1903_cov_2.694264, whole genome shotgun sequence.
ACCESSION   DAOOWR010000298 DAOOWR010000000
VERSION     DAOOWR010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60655_length_1903_cov_2.694264"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..551)
                     /locus_tag="PLB85_06745"
     CDS             complement(78..551)
                     /locus_tag="PLB85_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU73066.1"
                     /translation="MKITRKKRGSVSIPTASTGDIAFLLIVFFMSTTKFDVKEGVKVQ
                     LNKAVAQEQLQTTQLKLTEKEMTRLEIMETGLLKVNNDEPRSYSDAELDKLILEKIQL
                     RDSINRQETDPKLKSTKMLFLVKTNPEAKYSEMVRLVDHLVTYRDRAMISISTAI"
     gene            complement(564..1388)
                     /locus_tag="PLB85_06750"
     CDS             complement(564..1388)
                     /locus_tag="PLB85_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU73067.1"
                     /translation="MRKSFSKILPLIVLMSMLLVGLAYAQTETTTTAAAESDTAITEP
                     IPEEPVAETAPIEEKSSGLKGVAEFLFGRALVKEFIDGGWAMWPILFVAIYGLAYLIW
                     KFIALMYAKINLNDFLNKILALIKEKKIKEAIEFAKNTRGPVSAVVYAGLLKAERGVE
                     AVEKNMENAAMIEMSYLEKGFVEMTSAITLAPMLGFLGTVDGMIIAFDAIAKARSVDA
                     TIVADGIKVALITTKWGLIVAIPVQLLYNIFTTMVDGLVIDMQRASEKVTEALIES"
     gene            complement(1412..>1903)
                     /locus_tag="PLB85_06755"
     CDS             complement(1412..>1903)
                     /locus_tag="PLB85_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73068.1"
                     /translation="WDVRHNLSLNYTFRIGRGEEFFVPFTDFILPVDDLSANINWSFA
                     SGAPYTPQSVEGNSMLDTNSGRKKFTHQANLRITKGITLSQKMNLRLFMDVENLFGTR
                     NVYTVYPKTGSELHTGSEPDLADANTGYTYPEVRFVYDKYIQNPSFWSNYRGVTFGVS
                     FNF"
BASE COUNT          385 a          459 c          529 g          530 t
ORIGIN      
        1 cgatgtcgcc ggtcgaagtg gtgggaatgc tcacttccgg cttttctttc aacataatct
       61 tggccattgc tttgctccta tattgctgtg gagatggaga tcatggcccg gtcacggtat
      121 gtgaccaagt ggtcaaccag tctcaccatc tcgctgtatt tggcttcggg attcgtcttc
      181 accaggaaga gcatcttggt tgatttaagc ttggggtctg tttcctgcct gttgatggag
      241 tcgcgcaatt gtatcttttc caggataagc ttgtccagtt cggcatcgct gtaggagcgc
      301 ggctcatcat tattgacctt caacaatcct gtttccatga tctcgaggcg ggtcatttcc
      361 ttttctgtga gcttgagctg ggtggtctga agctgttcct gggctacggc tttgttcaac
      421 tgaaccttca cgccttcctt gacgtcgaac ttggtggttg acataaagaa gacaatcagc
      481 aggaaagcga tgtcgccagt cgacgcggtc ggaatactga cgctacccct tttcttgcgg
      541 gtgattttca tttttccccc ctatcaagac tcgatgaggg cttctgtaac cttctctgaa
      601 gctctttgca tgtcgatgac caagccatct accatggtgg tgaagatgtt gtaaagcaat
      661 tgcacgggaa tggcgacgat caagccccat ttggtggtga tcaaagcgac cttgataccg
      721 tcggccacaa tggtggcgtc cacagaacgg gctttggcga tggcgtcgaa ggcgatgatc
      781 ataccgtcaa ccgtacccaa gaaccccagc atgggggcca gggtgatggc tgaggtcatt
      841 tccacgaagc ctttctcgag gtaagacatt tcgatcatag cggcgttttc catgttcttt
      901 tccaccgctt ccacgccgcg ttcggctttc agcaggccgg catagacaac tgccgacacg
      961 gggccgcggg tgttttttgc aaattcaatg gcttccttga tctttttctc tttgatcagg
     1021 gccaggatct tgttcaggaa gtcgttgagg ttgatcttcg cgtacatcag ggctatgaat
     1081 ttccagatga ggtaagcgag gccgtaaatg gcaacgaaca ggatcggcca catggcccat
     1141 ccgccatcga tgaattcttt caccagggca cggccgaaca ggaattcagc cactcctttg
     1201 agtccggatg atttttcttc gatcggagcg gtttcggcta caggttcttc agggatgggt
     1261 tcggtgatgg cggtgtcgct ttcggcagcc gctgtggtgg ttgtttctgt ttgggcataa
     1321 gccaggccaa caagcagcat gctcatcagc actatcaggg gcagtatttt cgagaagctt
     1381 tttctcatgt tatctttcct ccaggggatt tctaaaagtt gaacgacaca ccaaaggtga
     1441 cgccgcgata gttgctccaa aaactggggt tttggatgta tttatcgtaa acaaatctaa
     1501 cttcgggata ggtgtagccg gtattggcat cggcaaggtc aggttcgctt ccggtatgca
     1561 gttcgctgcc ggttttggga tacactgtgt acacgtttct ggttccgaag aggttttcca
     1621 cgtccatgaa gaggcgaagg ttcatcttct gggagagggt gatgcctttg gtgatgcgca
     1681 ggttggcctg atgggtaaat ttcttgcggc cgctgttggt gtccagcatg ctgttccctt
     1741 ccacgctttg cggagtatag ggcgccccgg aggcgaagct ccagttgatg ttcgcgctca
     1801 ggtcatccac gggcaggatg aagtctgtga aaggaacgaa gaattcttcg ccgcggccta
     1861 tcctgaaggt gtagttcaag ctcaggttgt ggcgcacatc cca
//
LOCUS       DAOOWR010000299         1902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60699_length_1902_cov_2.783433, whole genome shotgun sequence.
ACCESSION   DAOOWR010000299 DAOOWR010000000
VERSION     DAOOWR010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60699_length_1902_cov_2.783433"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..>1902)
                     /locus_tag="PLB85_06760"
     CDS             complement(311..>1902)
                     /locus_tag="PLB85_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOU73069.1"
                     /translation="DAYVDHPSFGAAIIGRHLEAAGFRVGIIPQPDPARDGDFTVLGE
                     PRLFFGITAGNMDSMVCNYTAQRKKRNEDAYSPGGRAGLRPDRASLIYANVLKRLYKK
                     VPLVLGGIEASLRRLAHYDFWQNKVRGSLLADTKASLLVYGMAENAVVEIARSLRDGK
                     HANELTELPGTVAFSPEPPSEDDLVLPDNLACADKLTFHRMTHLFEENQHSRTLFQLN
                     GGRWLRHNPPAKTLSRNDMDALYALPFEHAPHPRYGQQKIPAFEQIRDSLTSHRGCYG
                     SCNFCAISSHQGRAIQSRSADSIVNEAKSLSTRRKKAITITDVGGPTANMYASSCELG
                     WPESCKRASCLYPKICPRLKPDHQEQLRLLEKLEHLPQVNHVFIASGIRHDLALRSPD
                     YISSLATKYTGGRLKLAPEHSVPGVLRLMGKPDVSSFEEFSREFLHTCKQAGLKRQII
                     PYIIIGHPGTTIQDALELRKWLIRNRLKVEQVQEFTPTPMTISTCMYYTGLDYYTGKP
                     IHVPKPSEIRRQKELALWHLN"
BASE COUNT          384 a          485 c          594 g          439 t
ORIGIN      
        1 cgtacttgaa tggggttcaa gtggtcgcag gttcaaatcc tgtcatcccg accaacacta
       61 tcagacccgg gtggaagccc gggttttgtt tttcccggtg gtttaccctt cctttgcccc
      121 ttccagtcca cctctttttc gcctcccgct gagttcccgc acttcaaacg ggaagttagc
      181 gggaagtgaa tgggttgtga gtggaatagg gcaaagggcg aataaacagc caacaaaagc
      241 tgaaatcagc ttggagcgag gcttttgatg aatgcagagg ctccgatgag tgaaagggtc
      301 ggggaggata tcaattcaaa tgccagaggg cgagttcttt ttggcgcctg atctctgaag
      361 gtttcggcac gtggatgggt ttgccggtgt aataatccag tcccgtgtaa tacatgcagg
      421 tgctgatcgt cattggcgtt ggggtgaatt cctgcacctg ttccaccttc aggcggttcc
      481 ggatcagcca tttgcggagt tccagagcgt cctggattgt ggtgccggga tggccgatga
      541 tgatgtaggg gatgatttgg cgcttgaggc cggcttgttt gcaggtatgg agaaactcac
      601 gggaaaactc ttcaaagctg ctgacatcag gctttcccat cagacgcaga acacccggca
      661 cgctgtgctc gggggcaagt tttagacgtc ctccggtgta ttttgtggcc agggatgaga
      721 tgtagtcggg gcttcgtaaa gccaggtcgt gcctgattcc ggaagcgatg aaaacgtgat
      781 tgacctgggg cagatgctcc agcttttcca gcagacgaag ctgctcctga tggtctggct
      841 tgagccgggg gcaaatcttc ggatagaggc aggatgcgcg tttgcagctt tccggccagc
      901 ccagttcaca gctcgaggca tacatgttgg cagtggggcc gccgacatcg gtgatggtta
      961 tggctttctt gcggcgcgtg gagaggcttt tggcttcgtt aacgatggaa tctgccgacc
     1021 gggactggat ggcccggccc tgatgtgaag aaatggcgca gaaattgcag ctaccgtaac
     1081 agccgcggtg cgaggtgagc gaatccctga tttgttcgaa ggccggaatc ttttgctggc
     1141 cgtagcgcgg atggggggca tgctcgaaag gcagggcgta aagcgcgtcc atgtcatttc
     1201 gcgaaagcgt tttcgctgga ggattatgcc gcagccagcg gccgccgttc aactggaaaa
     1261 gggttctgct atgctggttt tcctcgaaga ggtgggtcat gcggtgaaag gtgagtttgt
     1321 cagcgcaggc gaggttgtct ggcagcacca gatcatcctc ggaaggcggt tccggactga
     1381 aagccacggt cccggggagt tcggtcagtt cgtttgcatg tttgccgtcc cgcaaagacc
     1441 gggctatctc tacaaccgca ttttccgcca tcccgtaaac cagaagggag gctttggtat
     1501 cggccagcag gctgccccgc accttgttct gccagaaatc gtaatgggcc aatctgcgca
     1561 acgaggcctc gatgcctccc aataccagcg gaaccttttt gtagagccgc ttcaggacgt
     1621 tcgcgtagat caacgaagca cggtccggac gcaaacccgc cctgccgccg gggctgtagg
     1681 cgtcctcatt gcgttttttg cgctgggccg tgtagttgca gaccatggaa tccatgtttc
     1741 cagccgtgat gccaaaaaac aggcggggct ctcccaaaac ggtaaaatcc ccgtccctgg
     1801 cgggatcagg ctgtggaatg attcccacgc ggaacccggc tgcctccagg tgccggccaa
     1861 tgatggcagc tccgaagctg ggatggtcca cataggcgtc cc
//
LOCUS       DAOOWR010000300         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60744_length_1901_cov_3.087757, whole genome shotgun sequence.
ACCESSION   DAOOWR010000300 DAOOWR010000000
VERSION     DAOOWR010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60744_length_1901_cov_3.087757"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="topA"
                     /locus_tag="PLB85_06765"
     CDS             complement(<1..1320)
                     /gene="topA"
                     /locus_tag="PLB85_06765"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU73070.1"
                     /translation="MNKGLIIVESPAKAGTISKLLQNKFSVKASMGHVRDLPKHELGV
                     DVNNQFRPAYEIDKKKSKTIGELRAAAKEAPAVYLASDHDREGEAIAWHLARVLAKEL
                     AGKPVYRIVFNEITGKAITASIEEPGEIDMAKVDAQQARRVLDRIVGYTVSPLLWKVI
                     AKDLSAGRVQSVALRLICEREQEIGAFVPREFWKLEASFWKDEFPPFKASLEKWQGKK
                     FEIPDEAKAIEIAKEVSSSLAVISEIKRTLRNLQPQPPFITSTLQQEASKLLGFTSER
                     TMSIAQQLYEGIALKGETTGLITYMRTDSVRIATEAVENCRGLVKERFGADMLHDTAR
                     VYKNKSSAQDAHEAIRPTDAFHTPESVAASLSKEQLKLYTLIWQRFVATQMKPVKLEN
                     TSAKIGLGEAIFAASGNRVVEEGFMKAYPHVMFVEGVQIHTAYIKGDY"
     gene            complement(1334..>1901)
                     /locus_tag="PLB85_06770"
     CDS             complement(1334..>1901)
                     /locus_tag="PLB85_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOU73071.1"
                     /translation="VLVIAAFVTAVELVMQAYEKKEAQVGEEMMRDIERRSVLEVVDD
                     EWRDHLHEMDLLKESVHLRSYAQKDPLVEYKRESYELFENLISRIQDNVTKKVFTTYI
                     LSQEQMESMLNKAKTQHEDMSAFIQDQTLQVSQNASEPPQFSGPYGGEAAKPRPVHVA
                     PKVGRNDPCPCGSGKKYKNCCGRFADSE"
BASE COUNT          348 a          533 c          534 g          486 t
ORIGIN      
        1 atagtcgcct tttatgtaag cggtatggat ctgaacgcct tcaacaaaca tcacgtgggg
       61 ataggccttc atgaaaccct cttccaccac gcggttgccg ctggcggcga atatggcttc
      121 gcccaggccg atcttagctg aggtgttttc cagcttcacg ggcttcatct gcgtggcaac
      181 aaagcgttgc cagataaggg tgtaaagttt caactgctcc ttgctgaggc tagcggccac
      241 gctctccgga gtgtgaaagg cgtcggtggg ccggatagcc tcgtgagcat cctgagccga
      301 ggatttgttc ttgtaaaccc tggcggtgtc atgcagcata tcggcgccga agcgctcttt
      361 gaccaggccg cggcagtttt ccacagcctc ggtagcgata cgaacgctgt ctgtgcgcat
      421 gtaggtgatc aggccggtgg tttcgccctt gagggctatt ccctcataga gttgctgggc
      481 aatggacatc gtcctttccg aagtaaaacc cagcagtttg gaagcttcct gctgcagggt
      541 gcttgtgatg aaaggcggct ggggctgcag gttgcgcagg gtgcgtttga tctcactaat
      601 cacagccagg gaggaactga cttctttggc tatctcgatg gctttggcct cgtccgggat
      661 ctcgaatttc ttgccctgcc atttttccag actggctttg aaagggggaa actcatcctt
      721 ccagaaactg gcttccagct tccagaattc acggggcaca aatgccccga tttcctgttc
      781 ccgttcgcag atcaggcgca gcgccacact ctgcacacgg ccggcgctga ggtctttggc
      841 gataactttc cagagcaggg gcgaaacggt gtagcccacg atgcggtcaa gcactctccg
      901 ggcctgctgg gcatccacct tggccatgtc aatttccccc ggctcttcga tcgaggcggt
      961 gatggccttg ccagttatct cattgaaaac tatgcggtaa acaggcttcc ctgccagttc
     1021 ctttgccaaa accctggcca gatgccaggc tatggcctca ccctcgcggt cgtggtcgct
     1081 ggccagatag accgccgggg cttctttggc cgcggcgcgc agttcgccga tggtcttaga
     1141 tttctttttg tctatttcgt aagccggccg gaattggttg ttaacatcca ctcccagctc
     1201 gtgtttgggc aggtcacgca cgtggcccat agaggctttc acggaaaact tgttttgcag
     1261 gagcttggag attgtgcccg ccttggcggg ggattcaaca atgatcaaac ctttgttcat
     1321 ttcttcctct caatcattcg gaatcggcaa agcgcccgca gcagttcttg tatttcttgc
     1381 cgcttccgca gggacaggga tcgttgcgtc ccaccttcgg agcaacgtgc acaggccggg
     1441 gtttggctgc ttcaccgccg taaggtcctg aaaactgagg cggttcactg gcattttgcg
     1501 agacctgcaa agtctggtcc tggataaagg cgctcatgtc ctcatgctgg gttttggcct
     1561 tgttgagcat gctctccatt tgttcctggg agaggatgta ggtggtgaaa accttcttgg
     1621 ttacgttgtc ctggatgcgg gagataaggt tttcaaagag ttcgtagctc tcacgcttgt
     1681 attcgaccag cgggtctttt tgcgcgtagg aacgcagatg cacgctttcc ttcagcaggt
     1741 ccatctcgtg caggtggtcg cgccattcgt catccaccac ctccagcacg ctgcggcgct
     1801 cgatgtcgcg catcatctcc tcccctacct gggcttcctt tttctcgtag gcctgcatca
     1861 ccagttccac cgccgtgacg aaggcggcga tgaccagcac g
//
LOCUS       DAOOWR010000301         1896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_60982_length_1896_cov_3.701793, whole genome shotgun sequence.
ACCESSION   DAOOWR010000301 DAOOWR010000000
VERSION     DAOOWR010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60982_length_1896_cov_3.701793"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1896)
                     /locus_tag="PLB85_06775"
     CDS             complement(<1..>1896)
                     /locus_tag="PLB85_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOU73072.1"
                     /translation="LTGMDYPSASIDNLFIPLANPSLLCTGHASGLGWAQTLEDWKLR
                     RHYWLFANMDNLSYTLEYTRDAADNPMNYHTLALGSKVLPNSILPNLYIGTHYRWKNG
                     NIGRGDWRSALTYRPHGSTSLAFRLDNPYKESPAYHAGAALRPLAFVNGIREERLELS
                     ADIDYDKDGGGYGFNDFVIGAHTRLLDGLNVGASYNLDKKTAWMNFSLSFGKVDVGAV
                     ATSKNRVPYIHLSDNLFRPFLGLRGKNWYEMKLEGKIKTYTEPKFKIGKIRIMDNQSK
                     GIESLIADINRAKEDPSVHGILLKNPSFSTSFALLQELTDAFRDFKSGGKKITFYYDN
                     IGNSGYMFASSIADAIYLNPQGVVDLRGLSASSPYFKELLDTLGVEVMNFRSHKYKNA
                     GNVFSETEMTEAEREVYDSLLQSIYDQIVAAMDAGRGDKLKSSVQETIDAGPYFLASD
                     ALAAGLVDKIIYEDELKDILKDEFSFSGTQSCVPEYINYKWSQPRESLIAVIYAQGNI
                     VMGEGEPGSVIAHENTVKLIREARKNPMYKGIILRVDSGGGSGQASDIILRELQLAQA
                     ENKKPVVVSMSGMAGSGGYYIACGADRIVAQPSTLTGSIGVMGLTFALPRLYDKIGVN
                     WSTMQKG"
BASE COUNT          364 a          488 c          572 g          472 t
ORIGIN      
        1 cgcctttttg catcgttgac cagttcacgc cgatcttgtc gtagagcctg ggcagggcga
       61 aagtgagccc cataacgccg atggaaccgg tgagagtgga gggttgagcc acgatgcggt
      121 cggccccgca ggcgatgtaa tagcctccgg aacccgccat gccggacatg gataccacca
      181 ccggcttctt gttttccgct tgggcaagtt ggagttcgcg caggatgatg tcggaggcct
      241 ggccagatcc gccgccgcta tccacgcgca ggataattcc tttatacatg gggtttttgc
      301 gcgcttcgcg tatcagcttc acggtgtttt cgtgggcaat cacggagccg ggttcccctt
      361 cgcccatcac gatgttgccc tgggcataga taaccgcgat gaggctctcg cggggctggc
      421 tccatttgta attgatgtat tcgggaacgc agctttgggt gccggaaaag ctgaattcat
      481 ccttcagaat atcctttagc tcgtcttcat agataatctt gtccaccagg cccgccgcca
      541 gggcgtccga ggccaggaaa taggggccgg cgtcgatggt ttcctgaacg gagcttttca
      601 acttgtcgcc ccgtccggcg tccatggcgg ccactatctg gtcgtagatg ctctggagaa
      661 gggaatcgta aacctcgcgc tcggcctcgg tcatttcggt ttcggaaaac acgtttccgg
      721 cgtttttata cttgtggctg cggaagttca tcacctccac ccccagagtg tccagcaact
      781 ccttgaaata ggggctggag gcggaaaggc cacgcaaatc caccactccc tgtggattaa
      841 gatagatggc gtcagctatg gaggaggcga acatgtagcc tgaattgccg atattgtcat
      901 agtagaaggt gatttttttg cctccggact tgaaatcccg gaaagcgtcc gtgagttcct
      961 gcaaaagggc gaaggaagtg gagaaggagg ggtttttcag caggatgccg tgcacggagg
     1021 ggtcttcctt ggcccggttg atgtcggcta tcaggctttc gatgcccttg ctctggttat
     1081 ccattatcct tatcttgccg attttaaatt tcggctcggt gtaggtcttg attttccctt
     1141 ccaacttcat ttcgtaccag tttttcccgc gcaggcccag gaaggggcgg aagaggttgt
     1201 ccgataaatg gatgtaaggt actctatttt tactggtggc gacggcgcca acatcaacct
     1261 tgccgaagct gaggctgaag ttcatccaag cggttttctt gtccagattg taggaagctc
     1321 cgacgttcag gccatccaaa agtctggtgt gggcgccgat aacgaagtcg ttgaatccgt
     1381 agccgccgcc gtctttgtca tagtcgatgt ccgcgctcag ttccagcctt tcttccctga
     1441 tcccattcac aaaagccagg ggccgcaaag ccgcgccggc gtgataggca ggggattcct
     1501 tgtaggggtt gtccaacctg aaagccagcg aggtggagcc gtggggcctg taggttaggg
     1561 cgctgcgcca gtcgccccgg ccgatgtttc cgtttttcca gcggtaatgg gttcctatgt
     1621 agaggttggg cagaatgctg tttggcagca ctttgctgcc cagggccagg gtgtggtaat
     1681 tcatcgggtt gtctgccgcg tccctggtgt attcaagggt gtagctaagg ttgtccatat
     1741 tggcgaaaag ccagtagtgg cgacgtaatt tccagtcttc aagcgtttgt gcccagccaa
     1801 gtccgctggc gtggcctgtg cacagcaggg aggggttggc cagcgggatg aagaggttgt
     1861 cgatgctggc gctgggataa tccatcccgg tgaggt
//
LOCUS       DAOOWR010000302         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_62807_length_1856_cov_3.657968, whole genome shotgun sequence.
ACCESSION   DAOOWR010000302 DAOOWR010000000
VERSION     DAOOWR010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62807_length_1856_cov_3.657968"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLB85_06780"
     CDS             <1..399
                     /locus_tag="PLB85_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73073.1"
                     /translation="RKGTLVISQKKDISGKFITTAKYSYDTKRGIISILQDYLNDAHT
                     QKTTNVLDGEGRLKESTINDFQGNFMRKYTYSYDSEERLSSMLYTSPNDKINTMHTYI
                     TDDLGNLIRYSATQDKGDQNLSLSIRYEYY"
     gene            771..1496
                     /locus_tag="PLB85_06785"
     CDS             771..1496
                     /locus_tag="PLB85_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOU73074.1"
                     /translation="MIGKRMKALRLSLKVTQSALAKKLKVKPSAISQIESGKIRPSLD
                     TILLLTKVCDVNLHWLITGRGPMWVNPQDSRRKTKDKLNKVRDFIHGELDVLTRNREQ
                     TQLAELLDIPVSGEIAAGPPVENFETYMEFLTIRRTMIRGVVEDYVCLRVNGSSMEPM
                     VFNNDLVLIRQNQDWKQLDGQICALRIDGGITLKRLVMDEKEKMIILVSLNDEYQPLL
                     INPEEHQDITLIGSLYYLMRKVS"
     gene            1510..>1856
                     /locus_tag="PLB85_06790"
     CDS             1510..>1856
                     /locus_tag="PLB85_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73075.1"
                     /translation="MLLAILILCVLILVLGIVAAILLYRKQSAPDDRLDEVTRFSGQL
                     EAFERNLRDDLQRLRTDLLALGTDTRKELVATLDRQNENLNSENRKNREEINASLNNL
                     SQQINADAAKNRS"
BASE COUNT          516 a          494 c          461 g          385 t
ORIGIN      
        1 cgcaagggaa cactggtcat cagtcagaaa aaagacatca gcggcaaatt catcaccacc
       61 gccaaataca gctacgacac caagaggggc attatctcca tcctccagga ttatctcaac
      121 gacgcccaca cccagaaaac cacgaatgtc ctggatggcg aaggacgcct gaaagaaagc
      181 acgataaacg actttcaggg taactttatg cgcaagtaca catatagtta cgactccgag
      241 gaacgtctaa gcagcatgct ctatacctca cccaacgaca agataaacac catgcacact
      301 tacataaccg atgacctggg caacctcatc cggtacagcg ccacccagga caaaggagac
      361 cagaatctca gcctgagcat ccgctacgaa tattactgaa cgaaggcctt tttttgccac
      421 ctttccagtc cttgttgctc atttttgggt ccaattggta gaatagggat ggttttctct
      481 cacaccggac ctggtgaaat cgctggatac accgatccag gtgattagcg tagctgatag
      541 tttccgcttt agcgtaatga tatagggtaa tccttgcctt gactaatact ccctccagag
      601 gtgtttctca gcttcacatc ctaaatccgg gatttttgga tggctggcaa ctgctgttag
      661 cgtgaattac aaaaaagctc ttgacagagc agggcaaact ggaaaacttg ctccgagctg
      721 tcagttcccc gcgtggcggg gcgagagtaa gctcaaactg gaggaaaaac atgatcggaa
      781 aaagaatgaa ggccctgcgc ctgtcgctga aagtcacgca aagcgccctg gccaagaaac
      841 ttaaggtgaa gccatccgcc atttcccaga tcgaatcagg gaaaatccgc ccttctttgg
      901 acacgatact gctgctcacc aaggtctgcg atgtaaatct gcactggctg atcacgggca
      961 ggggtccgat gtgggtcaat ccacaggatt cgcggcgaaa aaccaaagat aaactgaata
     1021 aggtgcgcga cttcatccac ggcgaactgg acgttctgac gcgcaaccgg gaacagaccc
     1081 aattggctga attgctggat attccggtat ccggagaaat cgccgcagga ccgcccgtgg
     1141 agaattttga aacctatatg gagtttctca caatccgccg gaccatgatc cgcggggtgg
     1201 ttgaagatta tgtttgcctg cgcgtgaacg gcagcagcat ggagcccatg gtgttcaaca
     1261 acgatctggt gcttatccgc caaaaccagg actggaaaca actggatggg caaatttgcg
     1321 cgctgcggat cgacggcggc atcaccctga aacgcctggt gatggacgaa aaggaaaaga
     1381 tgataatcct ggtttcactc aatgacgaat accagcccct gctgataaat ccggaggaac
     1441 atcaggacat caccctgatc ggctccctct actatttgat gcgcaaagta tcataaggca
     1501 ggtgtaacaa tgcttttggc gatcctgata ctctgcgtgc tgatcctggt tctgggaatt
     1561 gttgccgcca tcctgctcta tcgaaaacag tcggctccgg atgacaggct ggatgaagtg
     1621 accaggtttt ccgggcagtt ggaagccttc gaacgcaatc tgcgcgacga tttgcagcgc
     1681 ttgcgcactg acctgctggc cctgggaacc gacacccgca aggaactggt ggccacgctg
     1741 gaccggcaga acgaaaacct aaattcagag aaccgcaaaa accgcgagga aatcaacgcc
     1801 tcgctcaaca acctgtctca acagatcaat gccgatgccg ccaaaaaccg atcgga
//
LOCUS       DAOOWR010000303         1840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_63635_length_1840_cov_2.907563, whole genome shotgun sequence.
ACCESSION   DAOOWR010000303 DAOOWR010000000
VERSION     DAOOWR010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63635_length_1840_cov_2.907563"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLB85_06795"
     CDS             complement(<1..669)
                     /locus_tag="PLB85_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOU73076.1"
                     /translation="MRWNAILGSVDKLLFPPVCLACNESVDSAAEVLCSQCRERLQPI
                     MENYCNKCGAPLADYRCEACSHLAFKFDYARAAYVFKTPAQELVYHLKYSDIRSPAVF
                     FSQALMSIPAAKRFRGNFDLVTAVPLHRVRERDRGYNQSELLGRALAFELGIPFSQPV
                     IRRQNTRSQTNLSRQARLDNLSGAFTLRRGANVGGQRIIVVDDVFTTGTTVNEVSQVL
                     KTGGA"
     gene            complement(635..1330)
                     /gene="purQ"
                     /locus_tag="PLB85_06800"
     CDS             complement(635..1330)
                     /gene="purQ"
                     /locus_tag="PLB85_06800"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOU73077.1"
                     /translation="MKVSVITFPGSNCDHDALEVFASRGHDISLVWHKDRDLQQPDLV
                     VLPGGFSYGDYLRGGALARFSPVVNEVLAYARRGGLVLGICNGVQILTECGLLPGTLL
                     MNSGLNFICRHQHVRVENSTTPYTEGIPSGTVLDMPIAHIDGNYFCDDHVYNELRDNN
                     HIVFRYCDASGNTTLEANPNGSRDNIAGICDPSGRVLGMMPHPERSATKFVTNQDGKR
                     FFDALERHFGFCG"
     gene            complement(1320..1589)
                     /gene="purS"
                     /locus_tag="PLB85_06805"
     CDS             complement(1320..1589)
                     /gene="purS"
                     /locus_tag="PLB85_06805"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOU73078.1"
                     /translation="MILAKIHVFLKPNVLDPQGKAVTNTLHQLGYSSVDETRVSKYIE
                     ISFNYSDPELAKRETEKICSNLLANPNTEHFHFTLEQREENQLES"
BASE COUNT          430 a          476 c          496 g          438 t
ORIGIN      
        1 agcgccgccc gtttttagca cttgcgagac ttcattaact gtggtaccgg tggtgaaaac
       61 gtcgtccacc acaatgatcc tttggccacc gacattcgct ccgcggcgca gggtgaaagc
      121 tccggaaagg ttgtccagac gtgcctggcg gctcaaattg gtctggctcc gggtgttttg
      181 acggcgaatc acaggttggg aaaaaggaat gccaagttcg aaggccaggg cgcggcccag
      241 aagttctgac tggttgtagc cccggtcgcg ttcgcgcacc ctgtgcagag gcacagccgt
      301 cacaaggtca aaattgcccc ggaaacgttt ggcagcggga atggacatca gggcctgact
      361 gaaaaaaaca gccggagacc tgatgtcgct atatttgagg tgatacacca attcctgcgc
      421 cggagttttg aaaacataag cagcccgcgc gtagtcgaac ttgaaggcca ggtgggaaca
      481 ggcttcgcag cggtagtctg ccagcggggc cccgcatttg ttacaatagt tctccataat
      541 tggctgcaaa cgctcgcggc actgggaaca gaggacctct gctgccgaat ccacagattc
      601 gttgcaagcc aggcacaccg gcgggaacaa aagtttatcc acagaaccca aaatggcgtt
      661 ccagcgcatc gaaaaacctt ttaccatcct ggtttgtaac aaactttgtg gctgaacgtt
      721 cgggatgggg catcattccc aacacccggc cggagggatc gcaaatgccg gcaatgttgt
      781 cccgggagcc gttgggattg gcttccaaag tagtgtttcc cgaggcatcg caatagcgaa
      841 aaactatatg gttgttgtcc cgcaattcgt tgtaaacgtg atcatcacag aaatagttgc
      901 cgtctatatg ggcgataggc atgtccagaa ctgtgccgga ggggatgcct tcggtgtaag
      961 gagtggtgga gttttccacc ctcacatgtt gatgccggca gatgaagttc aaaccgctgt
     1021 tcatcagcag cgtgccaggc aataggccac attctgtgag gatctggacc ccattgcaaa
     1081 tccccagcac caagccgccg cgccgggcat acgccagcac ttcgttcacg acgggtgaga
     1141 atctggccag cgctccgccg cggagatagt cgccatagga aaatccaccc ggaagcacta
     1201 ccagatcagg ttgttgtagg tcacggtcct tgtgccagac caaactgata tcatgccccc
     1261 gagaggcaaa aacctccagg gcatcatgat cacagttcga accggggaag gttatcacgc
     1321 taactttcaa gctggttttc ctcccgctgt tccagagtga aatggaagtg ttcggtgttg
     1381 ggattggcca gcagattact gcatatcttc tctgtttccc gcttcgccag ctccggatcc
     1441 gagtagttga aactgatttc gatgtatttg ctgacgcggg tttcatccac actggagtat
     1501 cccagctgat gcagggtatt cgtgaccgct tttccctgcg gatcaagcac gttgggtttg
     1561 agaaagacgt gtattttggc taagatcatt ctattaacct ttggcttttt atcagttatt
     1621 cacattctgg cggagccatt tttagtcaat ggattttttg caactgcctg gctcaacgcc
     1681 gcggaaacgc gatatttctt ccacaaccag cctatttggc agtgaaaggc ctgccaattc
     1741 tgtattacgc aaaggccttc tctattgaat atcgcatata ttattccggt tccggcgagg
     1801 taggagtttc aaccgtgaca tggccacgtt tcatcaggtg
//
LOCUS       DAOOWR010000304         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_64566_length_1820_cov_4.192068, whole genome shotgun sequence.
ACCESSION   DAOOWR010000304 DAOOWR010000000
VERSION     DAOOWR010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64566_length_1820_cov_4.192068"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(336..1292)
                     /locus_tag="PLB85_06810"
     CDS             complement(336..1292)
                     /locus_tag="PLB85_06810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOU73079.1"
                     /translation="MPKPKIFITRNIPQPALDRLAEVFDISIHANDKAIGRQQLMQDV
                     KDMDALLCLLTDSIDKELLEAAPRLKCVSNLAVGFNNIDLEYARSRNIAVCNTPGVLT
                     ETTADLTWALLLACARRVVEADNYARSGRFEGWEPLLMLGVDVFSKTLGIIGMGRIGS
                     AVARRAEGFGMKVLWFDPQVDPKTVSDKYERADLRHLCSSSDFISIHVPLTPDTHHLV
                     NSELLSLMRPTAVLINTARGPIVDEEALIKALRDKKIAAAGLDVFENEPEIPAELLEL
                     ENAVLLPHIGSASIETRTKMALMAAENAIAVIQGTEPPARVN"
     gene            complement(1295..>1820)
                     /locus_tag="PLB85_06815"
     CDS             complement(1295..>1820)
                     /locus_tag="PLB85_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73080.1"
                     /translation="NIDSSHPQARENLVYIQALNPNLPQEPRQPYLVQLFLNVYAYFS
                     LNRLAVMVLVLALLTTLSLHLLFHYPPERERGLPVLLVLAFGILLLFFSGALAVKHHR
                     WLHNPKAVVLRPAELRTGPGSGRMLKELVPATTVTIKSASGTQMQVVLPDGLSGWIDR
                     QAVEPVVPNQNYRR"
BASE COUNT          397 a          468 c          573 g          382 t
ORIGIN      
        1 tggaatagga tgaattcatc tcaattcaaa tgcgggtgta ttattcaacg gtcttctggg
       61 gtgaatcaat gcctttgccg gacaggcgga actcacgaag cttcgagcca acatcctgtt
      121 gtggagtggg atgaccttgc attcctcatg aaggcggtca agttccccac aaagttgcct
      181 cccgcacaat gcccgcattg aatgcgggca taatgcgggg agaatatgag agccgcaccc
      241 tgaggggcta agcagggagg caggctaaaa agaggacaag ccggacattg gcccggttaa
      301 tggcagggca ggtatcaggc gggaaaatcg ggatttcagt tgacccgcgc gggcggttcc
      361 gttccctgga tcacggcgat ggcattctcc gcggccatga gggccatttt ggtgcgggtt
      421 tcgatggagg cggagccgat gtgcggcaac aggacagcgt tttccaactc gagaagctca
      481 gccgggattt ccggctcatt ctcaaacaca tccagcccgg cggcggcgat ctttttatcc
      541 cgcaatgcct tgatcagcgc ttcctcatcc acaatcgggc cccgggcagt gtttatcagc
      601 acggctgtcg gccgcatcag ggagagcagt tccgagttga caagatgatg ggtgtcgggg
      661 gtcaggggga cgtggatgct gatgaaatcg gagctggagc agaggtggcg gaggtcagcg
      721 cgctcgtatt tgtcggaaac agttttggga tcgacctggg gatcgaacca gagcaccttc
      781 atcccaaaac cttcggcgcg gcgggccacg gcgctaccga tgcggcccat gccgatgatg
      841 ccaagggttt tggaaaaaac gtccactccc agcatcagca atggttccca gccttcgaaa
      901 cggcctgagc gggcatagtt gtcagcctcc accacgcgtc tggcgcaggc gagcagcagc
      961 gcccaagtga ggtcggcggt ggtttctgtg agcacgccgg gggtattgca gaccgcgatg
     1021 ttcctgcttc tggcgtattc caggtcgatg ttgttgaagc caacggcaag attggatacg
     1081 catttcagcc gcggggcagc ctccagcagt tctttgtcaa tgctgtccgt tagcaggcaa
     1141 agcagggcat ccatgtcctt tacatcctgc ataagctgct gtcggccaat ggctttgtcg
     1201 ttcgcgtgga tgctgatgtc aaaaacttcc gcgagcctgt ccagagcagg ctgggggata
     1261 ttgcgggtta tgaaaatctt gggtttgggc atggctatct cctataattt tggtttggga
     1321 ccaccggttc aaccgcctgt ctgtcaatcc agccgctaag gccgtccggt agaacaactt
     1381 gcatctgggt tcccgaggca gactttatcg ttacagtggt agcgggcacc aattccttca
     1441 gcattctgcc cgagcccggg ccagtccgca gttccgcggg ccttaaaaca accgctttgg
     1501 ggttatgcag ccaacggtgg tgcttcaccg cgagggcgcc gctgaaaaag agcagaagaa
     1561 tcccgaaggc aagcaccaaa agaaccggaa ggccacgctc ccgttccggt gggtagtgga
     1621 aaagcaaatg caggctgagc gtggtgagca gggcgaggac caggaccatg acagccagcc
     1681 ggttgaggga aaagtaggcg taaacattca ggaaaagctg caccagatat ggctgacgcg
     1741 gctcctgggg caggtttgga ttaagcgctt ggatgtaaac gaggttttcc cgggcctggg
     1801 gatgggagga atctatgttc
//
LOCUS       DAOOWR010000305         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_64794_length_1816_cov_3.652470, whole genome shotgun sequence.
ACCESSION   DAOOWR010000305 DAOOWR010000000
VERSION     DAOOWR010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64794_length_1816_cov_3.652470"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1708
                     /gene="gcvPA"
                     /locus_tag="PLB85_06820"
     CDS             359..1708
                     /gene="gcvPA"
                     /locus_tag="PLB85_06820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU73081.1"
                     /translation="MPYISNTEAERRQMLERIGAESFEELISAIPKHLRLQGLLKLEA
                     PLSELEMTRRIQERTCGNVCAQKASSFLGAGVYDHFIPAAVDSIVSRPEFFTAYTPYQ
                     AEVSQGTLQFIYEYQTMICELTGMEVANASMYDGASAAAEAILMAVRKNGLKKAILPA
                     SLHPEYVKVIKSYTEGIGVELITCKAKAGVMDIDDLTAKMDARVGSVVIQTPNFWGNI
                     EPLADLEKVVHSNPKALLIVAADPISLAILKAPGALGADIVVGEGQALGNSMYMGGPL
                     FGFFATKLNMARSMPGRIVGGTLDKDGKRAYTLTLQAREQHIRRAKATSNICSNESLC
                     TLAATVYLSLMGSDGLAEVATQSTQLTHYMAQKIASVPGFSLAWPNAAFFKEFVVNCP
                     VPASQVIADLLDHGIYAGVDLGTFGQDSRQLLIAVTEKKTVAEIDDYIETLRELNYE"
BASE COUNT          434 a          530 c          483 g          369 t
ORIGIN      
        1 ggctgtgaag tatgtgtggg caaatgcccc aagcaagcca tcaaaatgac ataattccaa
       61 gccgatacct ttctcaaggc gggagcgaat cccgcctttt ctttattcct ccttaactcc
      121 cgaagccttg cctgacgcac gccccttgaa ggattcccgc atcccatgcg ggcatactgc
      181 gggaggcgac acaaagccga attatagacc gctaaggctg tgatggccgc ggatggttcc
      241 ccatcctgaa actgcctccc tgccgcccgg caaggtgccc atgatttttg cgattgacac
      301 aaaccgccca ttcaaattta tagataaatt cagaatttca gacagctatg gagagataat
      361 gccttatatt tccaatactg aagccgaacg gcgccagatg ctcgagcgga tcggagcgga
      421 atcatttgaa gaactgattt cagcgattcc caagcatttg cgcctgcaag gccttttgaa
      481 gctggaagcc ccgctttcag agcttgaaat gacccggcgc atccaggaac gcacctgcgg
      541 aaacgtttgc gcgcaaaagg ccagttcctt tttgggggcg ggggtttacg accatttcat
      601 tcccgccgcg gttgacagca ttgtgtcccg accggagttt ttcaccgcct acacgcccta
      661 ccaggctgaa gtcagccagg gcactttgca gttcatatat gaataccaga caatgatatg
      721 cgaactcacc ggcatggagg tggccaacgc ctccatgtat gacggggctt cggcggcggc
      781 ggaagccatc ctgatggcgg tgcgcaagaa cggcctaaaa aaagccatcc tccccgccag
      841 ccttcatccc gaatatgtga aagtgataaa atcctacaca gaaggaatcg gggtggaact
      901 cattacctgc aaagccaagg ccggcgtgat ggatattgac gatttgacgg caaaaatgga
      961 cgccagagtt ggcagcgtgg ttatccagac ccccaacttc tggggcaaca tagagccgct
     1021 tgctgacctg gaaaaggtgg tccacagcaa tcccaaagct ttgctgatcg tagccgctga
     1081 ccccatctcc ctggcgattc tgaaagcgcc gggggccttg ggcgcggaca tcgttgtggg
     1141 cgaaggacag gctctgggca acagcatgta catgggcggc cccctgtttg gatttttcgc
     1201 caccaagctg aacatggccc gttccatgcc gggccgcatc gttggaggaa ccctggacaa
     1261 ggacggaaag cgggcttaca ccctcaccct ccaggccagg gaacagcaca tccggcgcgc
     1321 caaggccacc tcgaacatct gttccaacga atcgctctgc actttggcgg caaccgtcta
     1381 tctgagcctg atgggaagcg acgggctggc tgaagtggcc acccaatcca cccaactgac
     1441 acattacatg gcccagaaaa tcgcctccgt gcccggattt tccctcgcct ggcctaacgc
     1501 tgctttcttc aaggaattcg tggtaaactg ccccgtgccg gccagtcagg tgattgccga
     1561 cctgctcgac cacggcatct acgccggtgt ggacctgggc accttcggcc aggacagccg
     1621 ccagcttctg atagccgtca cggaaaagaa aacagtggct gaaatcgacg actacatcga
     1681 aaccctgcgg gagctgaatt atgagtaaga ccatttttga acacagcgtt gccggccgca
     1741 agggggtaac ccttccccgg cgggaactgg acacggaagt gaaagacttt tttccttccg
     1801 aatatctgcg cgaaac
//
LOCUS       DAOOWR010000306         1778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_66719_length_1778_cov_4.251886, whole genome shotgun sequence.
ACCESSION   DAOOWR010000306 DAOOWR010000000
VERSION     DAOOWR010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66719_length_1778_cov_4.251886"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..721
                     /locus_tag="PLB85_06825"
     CDS             152..721
                     /locus_tag="PLB85_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73082.1"
                     /translation="MRNIAILLAILLALAGCDLFRLRDSEPPVEQAPWNSYPTNWDLC
                     LQNLEYCFEDSRNAVKYAGLFTQDFSFRFAAQDVNDYNLPATWNAAQEQDMLLNLFSQ
                     SPGLENAEVILTPQDAQPDEIGPSEARIFRQYELVGFPAVAGATGHYKGNLEIQLRKS
                     GAYWYISKWFDYRTFANPTWGRMKHGFSQ"
     gene            705..1292
                     /locus_tag="PLB85_06830"
     CDS             705..1292
                     /locus_tag="PLB85_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73083.1"
                     /translation="MASRNRLLFLPLLALILLLSSCWNPFHPPLIDISDGSLRCGTPL
                     DVLQSLELAYKERNINIYKELLAPDFRFELISSEVGQIGIDVNGDGIRDSWWGYDQEV
                     LYTGNLFTSGSTDGSTPAPDELTLRLQIPPEANWEKDPELGHEDWVVIPTPFDLKLSF
                     YASNSMLTASGVARFYLKPANNRWYIAIWRDESNL"
     gene            1289..1708
                     /gene="ruvX"
                     /locus_tag="PLB85_06835"
     CDS             1289..1708
                     /gene="ruvX"
                     /locus_tag="PLB85_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU73084.1"
                     /translation="MRPPGRVLAVDYGSKRVGLALSDPLRLFAKPLKVIPNNGFAGIL
                     AEIRSTIAEQDVELLICGIPYAIEGGDTPKTTETKAFLAKLTAALEIRVLPWDERYST
                     DEAEKELIKLGYDWKKRRPIQDAMAAAMILKSYLETL"
BASE COUNT          392 a          536 c          454 g          396 t
ORIGIN      
        1 ctcataagga ccggattttg ccctttttgc ggatcaaccg gctattcaga aacggcttgt
       61 atgccatatc ctcagcggga taggatcaat tcggacgggc tttccgccaa atatctgttg
      121 acacgatttg cccgccgctg aaaatgagat tatgagaaat atcgccatac tccttgctat
      181 attgctggct ctggccggct gcgacctttt ccgcctgcgg gattccgagc cgcccgtgga
      241 acaggctccc tggaatagct atcccacaaa ctgggacctg tgcctacaaa acctggagta
      301 ttgctttgaa gacagccgca atgccgtgaa atacgctggc ctgttcactc aggatttcag
      361 tttccgcttc gccgcgcagg acgtgaacga ctacaatcta cccgcaacct ggaacgccgc
      421 tcaggagcag gatatgctgc tcaatctctt cagccagtca ccgggcctgg aaaacgcgga
      481 ggtcatcctc acgccgcaag acgcccagcc ggacgagatc ggcccttccg aggcccggat
      541 cttccgccag tacgagcttg tgggctttcc agccgtggcc ggcgccacgg gccattacaa
      601 gggcaacctt gagatacaac tgagaaaatc cggagcttat tggtacatca gcaaatggtt
      661 cgactaccgc acctttgcca accccacctg gggcaggatg aaacatggct tctcgcaata
      721 gactcctctt tcttccgctg ctggcgctga tcctgctgct ttcaagctgc tggaaccctt
      781 tccatccacc cctcatcgat atttccgatg gctctctccg ttgcggcacg cctctggatg
      841 tattgcaaag cctggaattg gcttacaaag aacgcaatat caacatctac aaggagttgc
      901 ttgccccgga tttccgcttt gaactgattt ccagcgaggt gggccagatc ggcatcgatg
      961 taaatggtga tggcattcgc gattcgtggt ggggatatga tcaggaagtt ctatacaccg
     1021 gaaacctctt caccagcggc tccaccgatg gcagcactcc cgctccggac gagctgaccc
     1081 tgcgactgca gattcccccg gaagccaatt gggaaaagga tcctgagctc ggtcacgagg
     1141 attgggtggt gatccctacg ccctttgacc tcaaactcag cttctatgct tccaattcca
     1201 tgttaacagc cagcggcgtt gcccgcttct atctgaaacc agccaacaac cgctggtaca
     1261 tcgccatctg gagggatgaa tcgaatctgt gaggccgccg ggacgcgtcc tggccgtgga
     1321 ttatggcagc aaacgggtag gtctggcgct ttcagacccg ctgcggcttt ttgccaaacc
     1381 cctcaaagtg attcccaaca atggttttgc cggcatcctg gctgaaatcc gctccaccat
     1441 cgcggagcag gatgtggagc tgcttatctg cggaatccct tatgccatcg agggtggcga
     1501 cactcccaag accactgaga ccaaagcttt tctggccaaa ctgacggccg cgctggagat
     1561 tcgcgtgctg ccctgggatg aacgctactc cacagacgag gcggagaagg aactgatcaa
     1621 gcttggctac gactggaaaa agcgccgccc catccaggac gccatggctg ccgccatgat
     1681 cctcaaaagc tatctggaaa ccctgtgaag atacctcccc gtcttgataa actcctgcgc
     1741 tggctgccgc caaccctcat cctagccttt gttctgag
//
LOCUS       DAOOWR010000307         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_66866_length_1775_cov_4.093605, whole genome shotgun sequence.
ACCESSION   DAOOWR010000307 DAOOWR010000000
VERSION     DAOOWR010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66866_length_1775_cov_4.093605"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLB85_06840"
     CDS             complement(<1..316)
                     /locus_tag="PLB85_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73085.1"
                     /translation="MKRIIWLTCLLLSLCLGAAALTIYDVQYTASRGVDGSYPSPWLG
                     KQVTLEGIVTATGYRGEGFFLSEKVSGAWRGIYVLDSRHHPAVGSYVRLTGEVAENYG
                     MTC"
     gene            523..1485
                     /locus_tag="PLB85_06845"
     CDS             523..1485
                     /locus_tag="PLB85_06845"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73086.1"
                     /translation="MKTNIVIKGASEHNLKNIDLEFPRDRLVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPRVDYIEGLSPAIAIEQKSASKNPRSTVGTV
                     TEIYDYLRVLYARVGRQHCHVCGDPVGSQTVDQMIEHLTRKPSGTKLQILAPLAQARK
                     GEHKEQLEELRQQGFARVLINGQTRSLDEEIALDKKSKHNIDVVIDRVVIKPGVESRL
                     ADSLELALKLTDGLVKIDFLEESEVQLLSSTNSCARCNIGFEELSPQHFSLTAPSAPA
                     RFATASATSWNSIRTSLCRTPNLPSWKALWPPGAGWKPNRTAGP"
     gene            1437..>1775
                     /locus_tag="PLB85_06850"
     CDS             1437..>1775
                     /locus_tag="PLB85_06850"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU73087.1"
                     /translation="MAPWGRLEAKQDSWTMNIVRNLAKAYDFSLTTPWYKLPEIIKQL
                     ILYGSKGKKFKNAWSNARGSGSFMMRHEGVIPTLGRRMHETSSEEMRRHYMQYISDKP
                     CPECGGKKLKP"
BASE COUNT          420 a          514 c          481 g          360 t
ORIGIN      
        1 tgcaggtcat gccgtagttt tcagcgactt caccggtgag gcggacatag cttcccacgg
       61 ccggatggtg gcggctgtcc agaacataga tgccccgcca ggcgccgctg accttttcag
      121 agagaaagaa tccctcgccg cggtagccgg tggcggtgac gatcccttcc agggtaacct
      181 gcttgcccag ccagggtgag ggataggagc cgtccacgcc ccgggaggcg gtgtactgga
      241 catcgtatat ggtgagggcc gcagccccca ggcagaggct gagtaacagg cacgtcagcc
      301 agataattct tttcattttg acctctgttc gccaatttgt atccgcttga tatattgtca
      361 agttaaatca agcttattct caaagccggg cgcaggtgaa agcgctatgg cttgttttgt
      421 agcaaattac gttccgtttt ccgccgcatc ttttgagtcc atacatttca tttgacaaat
      481 ccctgcctct gaaattcact gctcggacat gaggaacgaa acttgaagac caacatagtg
      541 ataaaaggcg catcagagca caatctcaag aacatcgacc tggaatttcc ccgcgacagg
      601 ctggtggtgt tcaccggagt ttccggctcc ggcaaatcct ccctggcctt tgacacgctc
      661 tacgctgagg gtcagcgccg ctatgtggaa tcgctttccg cctacgcacg tcagttcctg
      721 gggcagatgg aaaaaccccg cgtggactac atcgaggggc tttccccggc catcgccatt
      781 gaacagaaat ccgccagcaa aaacccacgc tcaacggttg gaaccgtcac cgaaatatac
      841 gattacctga gagtccttta cgcgcgggtg ggcaggcagc attgccatgt ttgcggcgat
      901 ccggtgggtt cgcagaccgt tgaccagatg attgaacacc tgacgcggaa accctccggc
      961 accaagcttc agatactggc tccgctggcc caggcaagaa aaggcgaaca caaggaacag
     1021 cttgaggaac tgcgccagca aggtttcgcg cgggtgctga tcaacggcca gacgcgcagc
     1081 cttgacgaag agatagctct ggacaagaaa agcaagcaca acatcgacgt cgtgatcgac
     1141 cgcgtggtca tcaaaccggg ggtcgaatcc cgtctcgccg attcgctgga actggctctg
     1201 aagctcaccg acggcctggt gaagatagac tttttggagg aaagcgaggt ccagttgcta
     1261 tcctccacca attcctgcgc acgctgcaac atcggtttcg aagagctttc gccccagcat
     1321 ttttccttaa cagccccatc ggcgcctgcc cggtttgcaa cggcctcggc tacaagctgg
     1381 aattcgatcc ggacctcgtt gtgccggacc ccgaacttac catcatggaa ggcgctgtgg
     1441 ccccctgggg caggctggaa gccaaacagg acagctggac catgaacatc gtgcgcaatc
     1501 tggccaaagc ctacgatttt tcactcacaa cgccctggta taaactgcct gagataatta
     1561 agcagctcat cctttacggt tccaaaggca agaagttcaa gaacgcctgg tccaatgcac
     1621 gcggctccgg cagcttcatg atgcgccacg agggcgttat cccgactttg gggcggcgca
     1681 tgcacgaaac cagctccgaa gagatgcggc gccattacat gcaatacatc agcgacaaac
     1741 cctgcccgga atgcgggggc aagaagctga aacca
//
LOCUS       DAOOWR010000308         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_69107_length_1735_cov_4.590476, whole genome shotgun sequence.
ACCESSION   DAOOWR010000308 DAOOWR010000000
VERSION     DAOOWR010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69107_length_1735_cov_4.590476"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLB85_06855"
     CDS             <1..1110
                     /locus_tag="PLB85_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOU73088.1"
                     /translation="GEPGDLPCLEIRDWPSYSWRGLHLDVSRHFFDFKFVKRYLDWMA
                     HAKLNKFHWHLSDDQGWRLESKKFPRLHEVGAWRKEANGSIHGGYYTRRQIKAVLDHA
                     ALRGIEVIPEIDIPGHAMAILAAYSDLACFPQDFETLNVWGISEDILCAGKDEVIDWL
                     KELFSEVAELFPGRYVHLGGDEAPKQRWQNCPHCQARIKKQGLAGEEALQGWLVQTLV
                     QHLKSLGKTVIGWDEILDGQPGQDPIVMVWRGDGIDAARMAHDNGNRYILSPYTKLYF
                     DARNSKYDTLGTHQIINWQDVLKFRFQDYTFQRKELLLGAQANAWTEHLANKADLKEK
                     VAHRLYPLAEILWNGEPVEDVMEFWNRWRDLGFRL"
     gene            1107..>1735
                     /locus_tag="PLB85_06860"
     CDS             1107..>1735
                     /locus_tag="PLB85_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73089.1"
                     /translation="MNRHPRNPLITRRDIVSSHPALQDVSSVFNPGGTWFDGRILLLL
                     RVQNRARETLFVKAWSENGVDFQVEDMPLPLLGLESCPHRIFHVYDPRITRLDGLYHV
                     ICAVDTDQGCFLGWFSTSDFAALEFRGLVSPADTRNGILFPEQVGGRYLRFERPNTLL
                     MVDGVKTGSGIVCASSDNLLNWSGAEKVFSGRPHFWDELIGSGPPPLKT"
BASE COUNT          369 a          533 c          483 g          350 t
ORIGIN      
        1 ggcgagcccg gcgacctgcc ctgcctggaa atccgggact ggcccagcta ttcctggcgg
       61 ggactgcatc ttgacgtgag ccgccatttc tttgatttca agttcgttaa gcgctatctg
      121 gactggatgg cgcatgccaa gctgaacaag ttccactggc atctttccga cgaccagggc
      181 tggcgcctcg aaagcaaaaa attcccccgc ctgcacgagg tcggcgcctg gcgcaaagag
      241 gctaacggca gcatccacgg aggttattac acccgccgcc agatcaaagc ggtgctagat
      301 cacgccgcct tacgcgggat cgaggtcatc cccgaaattg acatccccgg ccacgccatg
      361 gcgatactgg ccgcctattc tgatctggcc tgctttccgc aggattttga aaccctcaac
      421 gtctggggca tttccgaaga catcctctgc gctggcaagg acgaagtgat agactggttg
      481 aaagaattat tcagcgaagt agcggagctc tttcccggcc gttatgtgca tctgggcggc
      541 gatgaagccc ccaagcagcg ttggcagaac tgcccccact gccaggcccg gatcaaaaaa
      601 caaggcctcg ccggcgagga agcgctgcag ggctggctgg tgcaaaccct ggtccaacat
      661 ctcaaaagcc tgggcaaaac cgtgatcggc tgggacgaaa tcctcgacgg ccagcctggc
      721 caggacccca tcgtaatggt ctggcgcggt gacgggattg acgcggcccg tatggcccat
      781 gacaacggca accgctacat cctcagcccc tacaccaagc tctattttga cgcccgcaac
      841 agtaaatacg ataccctcgg cactcaccag atcatcaatt ggcaggatgt gctcaaattc
      901 cggttccagg actacacttt tcagcgcaag gaactcctgc tgggggccca ggccaacgcc
      961 tggaccgagc atctggccaa taaggccgat ctgaaggaga aagtcgccca ccgtctttat
     1021 cccctggcgg aaattctctg gaacggagaa cccgtagaag atgtaatgga attctggaat
     1081 cgttggagag atctgggatt caggctatga accgccatcc ccgcaatccg ctgatcacca
     1141 ggcgggacat cgtttccagc catcccgccc tgcaagacgt ttcctcggtc ttcaatcccg
     1201 gcggcacctg gttcgatggc cgaatcctgc tgctgctgag ggttcagaac cgcgcccgcg
     1261 aaacgctgtt cgtcaaggcc tggtccgaaa acggagtaga ttttcaagtg gaagacatgc
     1321 cgctgccgct gctggggttg gaaagctgtc cccaccgtat tttccacgtt tacgatcccc
     1381 ggataaccag gcttgacggg ctctatcacg tcatctgcgc tgtggatacc gatcagggct
     1441 gctttctggg ctggttttcc acgtcggatt ttgcagccct ggaattccgc ggcctggtct
     1501 cgcctgccga cacccgcaac ggcatcctct ttccggagca ggtcggagga cgctatctca
     1561 ggtttgaacg cccaaacacc ctgttgatgg ttgacggcgt gaaaaccggt tccggcatag
     1621 tttgcgcctc atcggacaac ctgctgaact ggtctggcgc ggagaaggtg ttttccggcc
     1681 ggccccactt ctgggacgaa ctgatcggct cgggcccgcc accactgaaa accga
//
LOCUS       DAOOWR010000309         1662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_73327_length_1662_cov_3.891101, whole genome shotgun sequence.
ACCESSION   DAOOWR010000309 DAOOWR010000000
VERSION     DAOOWR010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73327_length_1662_cov_3.891101"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLB85_06865"
     CDS             <1..1045
                     /locus_tag="PLB85_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOU73090.1"
                     /translation="QRNPDEIRDLGPIDLIVDNQNKPDIAHFLNATDNCFQDIMLCRD
                     FAYRSVTRMVEHSRAFQKIQDGCDFRCAYCAVPYGRGNSRSASFADVLGQARLFVANG
                     YREIVLGGVNLGLYRDGAKGLAEVVEALQELDGLDLIRLSSLEPMLFTPELIERISRC
                     DKLCPHFHIPLQSGCDSVLERMGRHYQTSKFKELIASILQTWPDAAIGLDVIAGFPSE
                     TEAEFQSTLDFLSELDIAYLHAFTFSRRWGTPADTLPGQIPNLVKNRRVKQLTALSDA
                     KKRAYTQMLLESKTPLRGVCEKVANGLATCLSDHFIRAYAAGDYTPGSLLKGIPSKLW
                     EDGLLLEPTPGQI"
     gene            1158..1233
                     /locus_tag="PLB85_06870"
     tRNA            1158..1233
                     /locus_tag="PLB85_06870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1191..1193,aa:Phe,seq:gaa)
     gene            complement(1466..>1662)
                     /locus_tag="PLB85_06875"
     CDS             complement(1466..>1662)
                     /locus_tag="PLB85_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU73091.1"
                     /translation="EHCEVRLFNIKGQELYSGRASRHHKGQGFELPKEALDPLPAGIY
                     LISLNPGNGKTATARLVVAE"
BASE COUNT          369 a          484 c          435 g          374 t
ORIGIN      
        1 ccagcgcaac cctgatgaga tcagggacct gggaccaata gacctaatcg tagacaacca
       61 gaacaagccc gatatcgccc attttctcaa tgcgacagac aactgctttc aggacataat
      121 gctctgtcgg gattttgctt accgttccgt gacccggatg gtcgagcaca gccgcgcctt
      181 ccagaagata caggatggtt gcgatttccg ctgcgcctac tgcgcggtgc cttacggacg
      241 cggaaacagc cgttccgcca gctttgccga tgtgcttggc caagcccggc tgttcgttgc
      301 caacggatac cgcgagatcg tgctcggtgg ggtcaatttg ggcctttaca gagacggagc
      361 taaaggtctg gctgaggttg tagaagccct gcaggagctc gatggacttg atctcatccg
      421 cctcagttcg ctcgagccaa tgctctttac ccctgagttg atagagagaa tcagccgttg
      481 cgacaaactc tgcccccact tccacatccc tcttcaaagc ggttgcgaca gcgtgctgga
      541 gcgcatgggc agacattatc aaacctctaa attcaaggaa ctgatcgcca gcattctgca
      601 aacctggccg gacgcggcaa ttgggctcga cgtgatcgcg ggtttccctt ccgaaacgga
      661 agcagagttc caaagcaccc tggatttcct ctctgagctc gacatagcct atctacacgc
      721 cttcactttt tcccgccgtt ggggcacccc cgcggatacc ctgccgggcc agatcccgaa
      781 tctggtcaaa aaccgccggg tgaagcagct taccgctttg agcgatgcaa aaaagcgagc
      841 ttacacccag atgctgctgg aaagcaaaac tcctctccgc ggcgtctgcg aaaaggttgc
      901 caatggcctt gccacctgtc tttccgacca tttcatccgc gcctacgctg ccggcgatta
      961 tactcctggc tccctgttga agggcatccc cagcaagtta tgggaggatg ggctgctgct
     1021 ggaacccacg cccggccaaa tctgaatcgt ttcagcccaa cagccctcaa accctttttc
     1081 caaacagcgg cggagccgtc accagggata attgtcttga caaaatcccg gctccaaaag
     1141 ttgatgcttt cacttgtggc gagatagctc agccggtaga gcagaggcct gaaaagcctt
     1201 gtgtccccag ttcaattctg ggtctcgcca ccagtaactt tcaggcgctt gatgaaagcg
     1261 ctttttttat tcggggactt gacgaaaact ttggggctca agttcaattg gagtaggtgg
     1321 aagcgctgga atgacggaca gtcctgatct tcaaagctat ctggctggcg tcgagcgagg
     1381 gacgagcatc gacgacagca gcaacgcctg aagtccatgc tcacagtttt cgctggacgt
     1441 caggcccccg atttgatagg aagctttatt cagccactac cagcctggca gtagcggttt
     1501 tgccgtttcc cggattcagg ctgatgaggt aaatcccggc gggcaaaggg tcaagagcct
     1561 ctttgggaag ctcaaagcct tgccctttgt gatgcctgct ggccctaccg gaatacagct
     1621 cctgtccctt gatgttgaac agcctgacct cacaatgttc gc
//
LOCUS       DAOOWR010000310         1644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_74477_length_1644_cov_3.188169, whole genome shotgun sequence.
ACCESSION   DAOOWR010000310 DAOOWR010000000
VERSION     DAOOWR010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74477_length_1644_cov_3.188169"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1319)
                     /locus_tag="PLB85_06880"
     CDS             complement(66..1319)
                     /locus_tag="PLB85_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU73092.1"
                     /translation="MRSAFQSIMNHKMRSLLTLTGIVIGVLAVVSMFSSVYALKHLIS
                     KNMEGMGWDLSVVIAADTPDQVFGHRSRRRALRRAEQSVRTISYDDYLALKSSLPHKS
                     SYAMIMSTAVMKLGNKDKQVQLRATDPSFFTNKTYPILHGRYYNQYENDNVLPAAVLG
                     YHFAREQYGNANPVGKVLQLGEHRLRVVGVLKDDQLSSGGGMNFNAWERKDELSAVYL
                     PLKYGAYRFGTNKGVHMIYLQAHDEEEFRELKTQARQLLLARHRMYPNFSFLDVGELI
                     LTITKEIEGYMEKWNITLIAIASISLIVGGIGLFSTLLISIQERMTEIGVRKSVGATE
                     KDIFYYFIFEALALALLGALLGILLAWIALSLISKAIDFPLYLPVQGVLVGLFFSLVT
                     GFVSGLYPALKAASVDPIKAIYYFS"
     gene            complement(1343..>1644)
                     /locus_tag="PLB85_06885"
     CDS             complement(1343..>1644)
                     /locus_tag="PLB85_06885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU73093.1"
                     /translation="RGRFGAFIACSNYPKCKFSRPKTTGITCPECHEGEINQRRNKQG
                     KPFWSCTRYPDCKYITNDKPLPIACPNCGHPFIQEKYSKAKGNYKQCPQCKSVME"
BASE COUNT          334 a          439 c          503 g          368 t
ORIGIN      
        1 tttaacagtg tgtcaagcca aatcccggtt gccaggtcca taccaccgca tcggggcaaa
       61 ttggtttacg agaaatagta aatggccttg atcggatcga cggaagcagc tttgagagcc
      121 ggatacaacc ccgaaacaaa gcccgtgacg agagaaaaga acaggcccac cagcacgccc
      181 tgaacaggca aatagagggg aaaatctatg gccttgctga tcaggctgag ggcgatccag
      241 gccagcagaa tccccagcag cgctcccaac agggccaggg ccagggcttc gaaaatgaaa
      301 tagtaaaaga tgtccttttc cgtggcgccc acgctttttc gcaccccgat ctcggtcatc
      361 cgctcctgga tcgagatcag cagggtgctg aaaagcccga tgccgccaac gatgagggat
      421 atggaggcga tggcgatgag cgtgatgttc cacttttcca tgtagccttc gatctccttt
      481 gtgatggtga ggataagctc ccccacatcc aggaagctga aattgggata catccggtgc
      541 cgagccagca gcagttgccg ggcctgggtt ttcagctcgc ggaactcctc ttcgtcatga
      601 gcctgaaggt agatcatgtg cacgcccttg ttggtcccga aacggtaagc gccatattta
      661 aggggcagat acaccgcgct gagctcgtct ttgcgttccc aggcgttgaa gttcatcccg
      721 ccgccgctgg aaagctggtc gtccttgagc acgcccacca cgcgcagacg gtgttccccc
      781 agctgcagaa ccttgcccac cgggttcgcg ttgccatatt gctcccgggc aaaatggtag
      841 cctaagacag ccgcgggcag aacgttgtcg ttctcatact ggttgtagta gcggccgtgg
      901 aggatgggat aggtcttgtt ggtgaagaag cttgggtcgg tggcgcggag ctggacctgt
      961 ttgtccttgt tgccgagctt catcaccgcc gtgctcatga tcatggcgta gctgctcttg
     1021 tgaggcaggg acgatttgag ggccaggtag tcgtcgtagc tgatggttct cacgctctgc
     1081 tcggcgcggc ggagggcccg gcgccggctt ctgtggccga aaacctggtc cggtgtatcc
     1141 gccgcgatca ccaccgaaag gtcccagccc atgccttcca tgttcttgct gataaggtgc
     1201 ttgagcgcgt aaacgctgga aaacatggac accacggcca gcacgccgat gacaattccc
     1261 gtgagggtca gcagcgagcg cattttgtgg ttcatgatgc tctggaacgc gctgcgcaag
     1321 gcgtcccagg ccggaacgcg cgttattcca taacgctttt gcactgcggg cattgcttgt
     1381 aattaccctt ggctttgcta tatttttcct ggatgaaggg gtggccgcag ttggggcagg
     1441 cgatgggcag cggtttgtcg ttcgtgatgt atttgcagtc gggataacgg gtgcaggacc
     1501 agaatggctt gccctgcttg ttgcggcgct ggtttatctc accctcatgg cattccgggc
     1561 aggtgatgcc ggtggttttg gggcgggaga atttgcattt gggatagttg ctgcaagcga
     1621 tgaaagcgcc gaagcggcct ctct
//
LOCUS       DAOOWR010000311         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_75983_length_1620_cov_4.518850, whole genome shotgun sequence.
ACCESSION   DAOOWR010000311 DAOOWR010000000
VERSION     DAOOWR010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75983_length_1620_cov_4.518850"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLB85_06890"
     CDS             complement(<1..309)
                     /locus_tag="PLB85_06890"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU73094.1"
                     /translation="MKKRLLVVTGGGDCPGLNAVIRAIVKRAAFEPDWEILGSIDAFN
                     GILLDPMQLMELTPKTVAGIHVQGGTIIGTTNRGGPFNWPVQNADGSWTTIDRGADLM
                     E"
     gene            complement(348..1502)
                     /gene="dxr"
                     /locus_tag="PLB85_06895"
     CDS             complement(348..1502)
                     /gene="dxr"
                     /locus_tag="PLB85_06895"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU73095.1"
                     /translation="MVGKLALLGATGSIGTSTLAVLREQAPRITLSLVSAHRDAQKLA
                     AVCREFSVSTAILTGITESSEQSSLRQAYPDLKLFFGEEELLKALRNEDYDVALNAIS
                     GSAGLRASFAIAEKKARLALANKESLVMAGHLLMPLLRKNQVELLPVDSEHSAIFQAI
                     GGHPSSEVRAIHITASGGSFRDLPLEDFARVTPQQALKHPNWSMGAKVTLDSATMFNK
                     ALEVMEAHWLYGLPYERIKAVLHPQSVIHSLVEFVDGSILAQMSTPDMKLPILYALSW
                     PQRWASRLVPTDLPCLSRLEFAPLAPERYPLFYLGLEVAKAGGILPTVLNSANEAALH
                     LFLDGKITFPQIHAICAAAAEAWPNNPEPSLEEIIEANRAVYQGIIRSHA"
BASE COUNT          342 a          443 c          485 g          350 t
ORIGIN      
        1 ttccataagg tcggctccgc ggtcgatcgt ggtccaggaa ccgtccgcgt tctgcacggg
       61 ccagttgaaa ggcccgccgc ggttcgtggt gccgatgatg gtgcctccct ggacgtggat
      121 tccggccacg gttttgggag ttagttccat aagttgcatg gggtcaagca ggatgccgtt
      181 aaaggcgtcg atgctgccca gaatttccca gtcaggttca aacgcggcgc gtttcacgat
      241 ggcgcggatg acagcattga gaccggggca atctccgccc ccggttacaa ccagcagcct
      301 ttttttcata ttatactcca attgtttaac aaattgattt ctccggctca ggcatgggat
      361 ctgatgatgc cctgatacac tgcgcggttg gcctcgatga tctcttccag actgggttcc
      421 gggttgttcg gccaagcttc cgcagcggcg gcgcagatgg cgtgaatttg cggaaaagtt
      481 atcttcccat ccagaaataa atgcagggcg gcctcgttgg cgctgttcag caccgtgggc
      541 aggatgcctc ccgctttggc gacctcaagt cccagataaa aaagaggata gcgttccggc
      601 gccaggggcg cgaattccaa tctggaaagg cagggcaaat cggtaggaac cagtcgcgaa
      661 gcccagcgct gaggccagga cagagcgtaa agtatgggca gtttcatgtc tggagtgctc
      721 atctgggcca ggatcgagcc gtccacgaat tccactaggg aatggataac agactgagga
      781 tgcagcactg ctttgatccg ctcataaggc agcccgtaaa gccagtgggc ctccatcacc
      841 tccagggctt tgttgaacat cgtggcgctg tcaagggtca ctttcgcgcc catcgaccag
      901 tttggatgct tcaaagcctg ttggggcgtg acccgggcaa aatcctcaag gggcaaatcg
      961 cggaaagagc cgccggaagc ggtgatgtgt atggccctga cctcgctgga cggatgtccg
     1021 cctatggcct ggaaaatggc gctgtgttcg ctgtccacgg gcagcagttc cacttggttc
     1081 ttcctgagca gcggcatcag caaatggccc gccatcacca gtgattcctt gttggccagg
     1141 gccagccggg cttttttctc cgcgatggca aagctggcgc gaaggccagc tgatccactg
     1201 atggcgttca gggcaacgtc ataatcctca ttgcgcaaag ctttgagcag ttcctcctcg
     1261 ccaaaaaaca gtttcaaatc gggataggct tggcgcaggg aagattgctc tgaggattct
     1321 gttattcccg tgagaattgc cgtggaaacc gaaaattccc ggcagacagc ggcgagtttt
     1381 tgcgcgtccc ggtgggcaga aacgaggctg agagtgattc ggggagcttg ttcgcgcaga
     1441 actgccaggg tggacgtgcc gatggagccc gtggcaccca gcaaggcaag tttgcctacc
     1501 atgtatagct tagagacagc ctctgcgaag aacccagttc gtccggcgag ccgtttcgcc
     1561 aggcgtaatc cagtcccaat gccttataac gcactcccag ccccgcggtg aagcaatccg
//
LOCUS       DAOOWR010000312         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_77437_length_1598_cov_2.372651, whole genome shotgun sequence.
ACCESSION   DAOOWR010000312 DAOOWR010000000
VERSION     DAOOWR010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77437_length_1598_cov_2.372651"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>1598
                     /locus_tag="PLB85_06900"
     CDS             246..>1598
                     /locus_tag="PLB85_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOU73096.1"
                     /translation="MIPILWYIAPIGAVSALIFAAIFFQQVKRANPGNARMIEIASYV
                     REGAFAYLKQQYKGVAWFFLGAFLVFLFMAQVLHVLHWLVPFAFLTGGFFSALAGYVG
                     MNMATLASNRTAQACSESLNKGLKIAFRGGAVMGLTVVGLALIDISAWFFILNRIGFP
                     LHTITVVMLSFGMGASTQALFARLGGGIFTKAADVGADLVGKVEAEIPEDDVRNPATI
                     ADNVGDNVGDVAGMGADLYESYAGSILATAALGLSAVTTLGSQFEAVRINFVIAPMVL
                     AGIGALLSILGVFMVSTKENAGTKELMFALNKSVYGSSVLIAIASFFVTRALLPADYW
                     FGAFISTVIGLVCGILIGLFTEFDTSHSYRPTRNIAAQGNYGPATVILEGIAVGMRST
                     LGPVLTIVAGILMAFICSHGFQSIEMGLYGIGFGAVGMLATLGVTLAMDAFGPIADNA
                     G"
BASE COUNT          320 a          460 c          442 g          376 t
ORIGIN      
        1 tcggaaatct ggaggatcag agcgtttgct tccttgcggt agtctgtgta ttccatgtat
       61 gagtttccta cttgggtcga aattgaacaa aatgtcaggg cagtaaatct cgtgcgggcg
      121 attttgtcaa atcatttgta aaatctcaga atttcattga caaataagcg gcaccccgta
      181 aaaaaagtcc gttcccagaa caagagcgga tttttcgcat ccacaattta tactgaggtg
      241 agattatgat tcctatacta tggtacatag ccccgattgg ggcggtttca gcgctgatat
      301 tcgctgcgat cttcttccag caggtgaaaa gggccaatcc cggcaacgcc aggatgatag
      361 agatcgcttc ctatgtgcgg gagggcgctt tcgcctatct gaagcagcag tacaaagggg
      421 tggcatggtt ctttttgggc gcgtttttgg ttttcctgtt catggcgcag gttttgcacg
      481 ttctgcactg gctggttccc ttcgcgttcc tcacgggagg ttttttcagc gccctggccg
      541 gatacgtggg catgaacatg gccacgctcg cctccaaccg gaccgcccag gcctgttccg
      601 aaagcctgaa taaaggcctg aaaatcgcct tccggggcgg cgccgtgatg ggcctcaccg
      661 tggtggggct ggcgcttatc gacatttccg cctggttctt catcctcaac cgcatcggct
      721 ttcctctcca caccatcacc gtcgtgatgc tcagtttcgg catgggagca tccacccaag
      781 ccctcttcgc caggctgggc ggaggaatct tcaccaaggc cgcggatgtg ggcgcggacc
      841 tcgttggaaa agtggaagcc gaaatcccgg aagacgatgt gcgcaacccg gccaccatcg
      901 ccgacaacgt tggcgataac gttggtgacg ttgccggtat gggtgccgac ctctatgaaa
      961 gctacgccgg ctccatcctc gccaccgcgg ctttggggct gagcgccgtg accaccttgg
     1021 gatcgcagtt cgaagctgta aggatcaact ttgtcatcgc gccaatggtc ctggccggga
     1081 taggggcgct gctctccatc ttgggagttt tcatggtgag caccaaggaa aacgccggca
     1141 ccaaagagct gatgttcgcc ctcaacaaaa gcgtttacgg aagttccgtc cttatcgcca
     1201 tagcttcatt tttcgttacc cgagcgctgc tgccggcgga ttactggttc ggagccttca
     1261 tctccacagt gataggcctc gtctgcggaa tcctgatcgg cctcttcacc gagtttgaca
     1321 catcccacag ctaccgcccc accagaaata tcgccgccca gggaaactac gggcccgcaa
     1381 ccgttatcct ggaaggaata gccgtcggca tgcgctccac cctggggccg gtgctcacga
     1441 tcgtggccgg aatcctcatg gccttcatct gcagccacgg cttccaatcc atcgaaatgg
     1501 gcctctacgg catcggtttc ggtgcagtgg gcatgctggc cacgctggga gtgactttgg
     1561 ccatggacgc ctttggcccc atcgccgaca acgccggc
//
LOCUS       DAOOWR010000313         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_77493_length_1597_cov_2.808690, whole genome shotgun sequence.
ACCESSION   DAOOWR010000313 DAOOWR010000000
VERSION     DAOOWR010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77493_length_1597_cov_2.808690"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1048)
                     /locus_tag="PLB85_06905"
     CDS             complement(149..1048)
                     /locus_tag="PLB85_06905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU73097.1"
                     /translation="MEKNPALICSLGVFAYNEADNIIPLLDSLCNQNLKQASIAEIIV
                     VSSASTDGTDELVENYTSRDPRVRLIRQARREGKSSAINLFIAEAKSPILIVISGDVI
                     PAPTTIEKLAVAFNDPKVGASGGRPVPVNPVNTFMGFAVHLLWRLHHRMAMISPKLGE
                     MIAFRKLMDSIPAESAVDEASIEAIVRSHGLKLRYVPDAIISNKGPTNLKDFIKQRRR
                     IQNGHLWLKQKQDYKVVSQDGGTLLWILLAELREYPASAPKVFAVMLLEMYCRLLGSW
                     DFYIKGKNPFAWDIARSTKDLGR"
     gene            complement(1051..1527)
                     /gene="nusB"
                     /locus_tag="PLB85_06910"
     CDS             complement(1051..1527)
                     /gene="nusB"
                     /locus_tag="PLB85_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU73098.1"
                     /translation="MGLRRKSRELAAQTLYALDFRELDPEFLEYGMLNSYPDVLNELA
                     KLEKMRNSATVIDFADDLVKNAIINRTEIIQKIEKHSENWSVESISHMDRNVLQVAVY
                     ELLYTDTPPAVVINEALEIGKKFCEEGAVKFINGVLDSVNKELRAKESIEGIARLR"
BASE COUNT          353 a          425 c          427 g          392 t
ORIGIN      
        1 agacattaca ggtgctgcag cgcgaattac aggtgtagag caggcttacg gtataactca
       61 tgggcaggag gcggggaaaa ccgagatgct tcatcagcca gtatccgagc atcctgaaaa
      121 gcagattgag catggcgctc aaaaatcctt aacgtcccaa atccttggtg gaccgggcga
      181 tatcccaagc gaaggggttt ttgcccttga tgtaaaaatc ccaagagccc aaaagccggc
      241 aatacatttc caaaagcatc acggcaaaca ctttgggtgc agaagccggg tattcgcgca
      301 attcggccag gagtatccag agcagggtgc ccccatcctg ggaaaccacc ttgtagtcct
      361 gtttttgctt cagccagaga tggccgttct ggatgcggcg gcgctgcttg atgaagtctt
      421 tcaggttcgt ggggcctttg ttgctgatga tcgcgtcagg aacgtagcga agtttcagac
      481 catgggagcg gacaatggcc tcgatgctgg cttcgtcaac cgcggattcg gccgggatgg
      541 aatccatcag cttgcgaaag gctatcattt cacccaattt gggcgaaatc atggccatgc
      601 ggtgatgcaa gcgccagagc agatgcacag cgaaacccat aaaagtgttg acgggattca
      661 ccggaacagg cctgccgccg ctggccccaa ccttagggtc attaaaagct acggccagtt
      721 tttcgatggt tgtgggcgcc gggataacgt ctccggaaat cactatcagg atcggcgatt
      781 tagcctccgc aataaatagg ttgatggcgc tggatttgcc ttcccgcctt gcctggcgga
      841 tcaagcgcac ccgcgggtcc cggctggtat aattctccac cagttcgtcc gtgccgtccg
      901 tgctggcgct ggaaaccaca atgatttccg cgatgctggc ctgctttaag ttctgattgc
      961 aaagcgagtc cagcaggggg atgatgttat ccgcttcgtt ataggcaaac acgcccaaag
     1021 agcagatcaa agctgggttc ttttccattt ttacctcagt cgggcaatgc cctctatgct
     1081 ttccttcgcg cgcagttctt tgttcacgga atccaggacc ccgttgataa acttcaccgc
     1141 gccttcctcg cagaatttct tgccgatttc gagcgcttcg ttgatcacca cagccggcgg
     1201 agtgtccgta tagagcaatt catatacagc tacttgcagc acgtttctgt ccatgtggga
     1261 gatactttcc acgctccagt tttcgctgtg tttctctatt ttctggatga tctcggtgcg
     1321 gttgatgatg gcgtttttca ccaaatcgtc ggcaaaatcg atcaccgtcg ccgagttgcg
     1381 catcttttcc agtttggcga gttcattcag cacatcggga tagctgttca gcatcccgta
     1441 ttccaagaac tccgggtcca gttcccgaaa atcaagcgcg taaagggttt gggccgccag
     1501 ttcgcgggat ttacgcctta gtcccaagct tgagttccgc catcaggttg agcatttcca
     1561 aagccgcgct ggcagcggaa aaacctttgt ttccggc
//
LOCUS       DAOOWR010000314         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_77829_length_1592_cov_3.309044, whole genome shotgun sequence.
ACCESSION   DAOOWR010000314 DAOOWR010000000
VERSION     DAOOWR010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77829_length_1592_cov_3.309044"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLB85_06915"
     CDS             complement(<1..191)
                     /locus_tag="PLB85_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73099.1"
                     /translation="MKKAALILALLSIVMLTALSARNKSAMVSIQIDPNPMTKATNVT
                     LLFNQAIPISVTIETDTGML"
     gene            complement(254..1177)
                     /locus_tag="PLB85_06920"
     CDS             complement(254..1177)
                     /locus_tag="PLB85_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU73100.1"
                     /translation="MTSDKATLGLDIGGSSVKYGWGDCKVGLQYFGKLELRQKSLPHL
                     RETVRQILDICARELGLSSLGAIGIGTPGTIDRNSGKIVGVNPNLPFWVDTDPRELIP
                     KELGLPVFCDNDANLMCLAEAWLRGGQGSVAGITVGSGIGCGFVVDGMIWHGAHGYAL
                     ELGHITSISQGELCSCGRKGCLEAYASVEGLRHRLLELPGADDFITESSNLSSLLAFS
                     QASPAAANIIVEGRDVLACGISDLIVILDPDAVVIGGGAMDGGLYPWNELKRLVKENL
                     PPVNEERTSLEQAREGNRAGVLGAVILASQS"
     gene            complement(1193..1267)
                     /locus_tag="PLB85_06925"
     tRNA            complement(1193..1267)
                     /locus_tag="PLB85_06925"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1233..1235),aa:His,seq:gtg)
     gene            complement(1304..1380)
                     /locus_tag="PLB85_06930"
     tRNA            complement(1304..1380)
                     /locus_tag="PLB85_06930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1344..1346),aa:Arg,seq:tct)
     gene            complement(1392..1468)
                     /locus_tag="PLB85_06935"
     tRNA            complement(1392..1468)
                     /locus_tag="PLB85_06935"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1432..1434),aa:Met,seq:cat)
     gene            complement(1516..1591)
                     /locus_tag="PLB85_06940"
     tRNA            complement(1516..1591)
                     /locus_tag="PLB85_06940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Met,seq:cat)
BASE COUNT          353 a          458 c          436 g          345 t
ORIGIN      
        1 agcatgccgg tgtctgtttc gatggtaacg ctgatcggga ttgcctggtt gaacagcaga
       61 gtgacgttcg tggccttggt catggggttg ggatctatct ggatggaaac catggcgctt
      121 ttgtttctgg cggaaagggc ggtcagcatt acaatgctga gcaacgcaag gatcaaggct
      181 gcctttttca tttgttgttt ctcctaaaag atgatgccga ttatttaagc aacttttgtt
      241 cccaatctct gaattaactt tgggatgcca atatcacagc gcccaaaact cccgcccggt
      301 tgccttcgcg agcctgttcc agggaggtgc gctcttcgtt cactggggga agattttcct
      361 ttacgagccg cttcagttca ttccagggat agagcccgcc atccatcgcg cctcctccga
      421 tcaccacggc gtcggggtcc aggatcacga tgaggtcgga aatgccgcag gccaggacat
      481 ctctgccttc tactataata ttggccgccg cgggactggc ctggctgaaa gcgagcaggc
      541 tggacaggtt ggaactctcc gtgatgaaat catccgcccc aggcagctcc aacagcctgt
      601 gccgcagccc ctcaaccgac gcgtaggcct ccaggcaacc ctttctgccg caggaacaaa
      661 gctctccctg agaaatgctg gtgatgtggc caagctcgag ggcatatccg tgcgccccgt
      721 gccagatcat gccatccacc acaaagccgc aacctattcc gctgccgaca gttatcccgg
      781 ctacgctgcc ctggccgccc cgcagccagg cttccgccag acacatcagg ttggcgtcgt
      841 tatcgcagaa aacgggcagg ccaagttctt tggggatgag ttcacggggg tcggtgtcaa
      901 cccagaaagg caggttggga ttgacgccca cgattttgcc ggaattccgg tcgatggtgc
      961 cgggagtgcc gatgccgata gcgccgaggc tggacagacc aagttcccgg gcacagatat
     1021 ccagtatttg gcgcacggtt tcccgcaggt gaggaaggct tttttgccgc agctcaagtt
     1081 tgccgaaata ctgtaaacca actttacagt cgccccagcc gtatttaacg ctggagccgc
     1141 cgatatccag tccgagtgtc gctttatcgc ttgtcaacat atcataaaaa ggtggggtgg
     1201 gtgatgggag tcgaacccac aacgcccgga accacaatcc ggtgctctgc cattgaacta
     1261 cacccaccac ggaaaggatc gatgtattta tgcgcttata agctggcacg ccagaaggga
     1321 ttcgaacccc tgacccgcag cttagaaggc tgctgctcta tccgactgag ctactggcgc
     1381 caaacaaaaa ctggtagcgg cgcagggact tgaaccccgg acctgcggat tatgattccg
     1441 acgctctaac caactgagct acaccgccac aagtcttaac gtaaaatccg gcgtcaagcg
     1501 ccggcatgta caaaatggta gcgagggaag gattcgaacc ttcgaccttc gggttatgag
     1561 cccgacgagc taccagctgc tccacctcgc gt
//
LOCUS       DAOOWR010000315         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_78406_length_1584_cov_3.418574, whole genome shotgun sequence.
ACCESSION   DAOOWR010000315 DAOOWR010000000
VERSION     DAOOWR010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78406_length_1584_cov_3.418574"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="PLB85_06945"
     CDS             complement(<1..>1584)
                     /locus_tag="PLB85_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73101.1"
                     /translation="LGIRGDRSLDYPFSWQNRYALDTYAVSETEQRQYVANYEYAFNP
                     AMNIKVKGSYYQKDSKTGPRGIDRSNYLYMYDGLDPENPGEDYVDNVLLGNQGWDSVD
                     HNSDGVYSPDYNGDGISDADYFDYSFWTYRIASVEDPRMVTGFTPPGYIYQFFQDDIT
                     TSASARADFEWQVNETHLAKTGLELIKHSIKKDQLQNFLNIYEDRFQASLKRKWNGLA
                     DFVPEYDADGNITNVPNELYSMYQTDDGVLIPIYKPADYFKAAQEASGKRDGYQADPW
                     QAAYYLQDKMVWEGMIVNAGLRLDLWYLGTKYKVLQDDASYREVDFDPSERWQLMLSP
                     RLGVSHPITDRDVLRFAYNYQNQLPQMQFIFTSKTPADANVSDQTITVGNTKLEPQIT
                     KTYEVGLSHQLSDDYVLDMTAYYKNLYNYVSTMKVAKPGEEQIFWYQFISEDYGSARG
                     IDVQLEKMMSNFNNWSVAYSLAWAQGNNSSTVIQDEATNLREFPLDWDVRHNLSLNYT
                     FRIGRGEEFFVPFTDFILPV"
BASE COUNT          311 a          387 c          484 g          402 t
ORIGIN      
        1 cacgggcagg atgaagtctg tgaaaggaac gaagaattct tcgccgcggc ctattctgaa
       61 ggtgtagttc aggctcaggt tgtggcgcac atcccagtcc agcggaaatt cgcgcaggtt
      121 ggtggcttcg tcctggatca cggtggagga gttgtttccc tgcgcccagg ccagagagta
      181 ggccacggac cagttgttga agttagacat cattttttcc aattggacgt cgattccgcg
      241 ggcggaaccg tagtcctcgg aaatgaactg gtaccagaag atctgctctt cgccaggttt
      301 ggctaccttc atcgtgctca cgtagttgta aaggttcttg taatatgcgg tcatgtcaag
      361 cacgtagtcg tcggaaagct ggtgggaaag gcccacttcg taggtcttgg tgatctgcgg
      421 ctccagcttg gtgtttccga cagtgatggt ctggtcggaa acattggcgt cggcgggggt
      481 tttggaagtg aagatgaact gcatctgagg caactggttc tgatagttgt aggcaaagcg
      541 cagcacgtcg cggtcggtga tggggtgtga aacgcccagg cgcggggaaa gcataagctg
      601 ccagcgctcg ctggggtcga aatccacttc ccggtagctg gcgtcgtctt gcagcacctt
      661 gtatttggtt cccagatacc agaggtcaag acgcaggccg gcattcacga tcatgccttc
      721 ccataccatc ttgtcctgga ggtagtaagc cgcctgccag ggatcggctt gatagccgtc
      781 gcgtttgcct gaagcttcct gggcggcttt gaaataatca gcgggtttgt agatgggaat
      841 caatactcca tcgtcggttt ggtacattga gtaaagttca ttaggcacgt tggttatgtt
      901 gccgtctgca tcatactcgg gaacaaaatc cgccaggccg ttccatttgc gtttcaggct
      961 ggcctgaaac ctgtcttcat agatattcag gaaattctgc agctggtctt tcttgatcga
     1021 gtgcttgatc agctccaggc cggtcttggc cagatgggtt tcgttaacct gccattcaaa
     1081 gtcggcccgg gcgctcgctg aagtggtgat gtcgtcctgg aaaaactgat agatgtagcc
     1141 ggggggcgtg aagcccgtca ccatgcgcgg gtcttccacg ctggcgatgc ggtaggtcca
     1201 gaaagagtaa tcgaagtaat cggcatcgct gatcccgtca ccgttgtagt cagggctgta
     1261 cacaccgtct gagttgtggt ccacgctgtc ccagccctgg ttgcccagca gcacgttgtc
     1321 cacgtagtcc tcgccgggat tttcaggatc caggccatca tacatataga ggtagttgct
     1381 gcggtcgatc ccgcggggtc cggttttgct gtccttttgg taatagctgc ctttcacctt
     1441 gatgttcatg gccgggttga aagcgtattc gtagttggcg acatactgcc tctgttcggt
     1501 ttcgctcacg gcataggtgt caagcgcgta gcggttttgc caggagaagg ggtaatcgag
     1561 ggagcggtcg ccgcggatgc ccag
//
LOCUS       DAOOWR010000316         1545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_81256_length_1545_cov_1.995973, whole genome shotgun sequence.
ACCESSION   DAOOWR010000316 DAOOWR010000000
VERSION     DAOOWR010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81256_length_1545_cov_1.995973"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLB85_06950"
     CDS             <1..423
                     /locus_tag="PLB85_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU73102.1"
                     /translation="PLLAIHFLDKYNKRMDKNVLGISDDAMKTLEQYEWPGNVRQLEN
                     EIERSVTLVEDGSFIKSSDFSDQVCRFMENKKTIGLLSTKKTLKDALEDLERKMIAET
                     MERLDWNQSHAARELGISRQGLIQKLKRYNLFKEENGE"
     gene            436..>1545
                     /locus_tag="PLB85_06955"
     CDS             436..>1545
                     /locus_tag="PLB85_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOU73103.1"
                     /translation="MATEKDFRNSLDFCAIDIESTGLKPERAEIIELAAIRFRNGEEV
                     ARFHTLVKPVRDMPKFIEYLTNISPQDLKAAPPPKEALKDFVDFVGDDILAGHNVGFD
                     LGFINYHWAVHGGITLANRNWDTAEISRVWLPYTSDHKLATLVKHFGITLGNAHRADA
                     DATATGELLIRLSEHILAHYPLLVNARLLDLSKQARLDDSLYSYIYKLVEYQRRFALS
                     GPKPKLPEAMKPNVLEHHGNGDPRLGIEEVFAEQGLFSQKFPNFEFRAGQLEMAMEVE
                     ESFRSQRHLAVEAGTGVGKSFAYLMPAIAFAEQQKARVVVSTNTKNLQEQLFFKDLPQ
                     LRDMLPLPFKATLVKGRENYVCERRWQEMMMEQTRG"
BASE COUNT          417 a          374 c          420 g          334 t
ORIGIN      
        1 ccgcttttag ccatacactt tttggacaaa tataacaagc gcatggataa gaatgtgctc
       61 ggcatctccg acgacgcgat gaaaaccctg gaacaatatg agtggccggg caatgtgcgt
      121 cagttggaaa atgagataga gaggtcggta actctggtgg aagacggatc cttcattaag
      181 agttccgatt tttcggatca ggtgtgccgc ttcatggaaa acaaaaagac aatcggcctg
      241 ctttcaacca aaaagactct caaagacgca ttggaagacc tggaacgcaa gatgatagcg
      301 gaaacgatgg aacggctgga ttggaatcag agccacgccg cccgcgaact gggcatctcg
      361 cgccagggac tgatccagaa gctcaagcgc tataacctct tcaaagaaga aaatggcgaa
      421 taggaggtat tgggcttggc aaccgaaaaa gatttccgaa acagtctgga tttttgcgcc
      481 attgacattg aaagcaccgg cctgaaacca gaacgggctg agatcataga attggcagcg
      541 atccgtttcc gcaacggtga ggaagttgca cgcttccata ccctggttaa gccggtacgg
      601 gatatgccaa agttcatcga atatctcact aatatttcac cccaggattt gaaagcagct
      661 ccgcctccaa aggaagctct gaaggacttt gtggacttcg tgggcgatga tattctggcg
      721 ggtcacaacg tgggcttcga cctgggcttt atcaactacc actgggctgt gcatggtggc
      781 ataactctgg ctaaccggaa ttgggacact gccgagattt cgcgggtgtg gttaccttac
      841 acatctgacc acaagttggc aacgctggtc aaacattttg gcatcacact gggaaatgct
      901 catcgggcag atgccgacgc cacagccaca ggagaactgt taattaggct gagcgagcac
      961 atcctcgccc actatcccct gttggtgaac gcccgcctgc ttgatttgag caaacaagcc
     1021 aggttggatg attcgctcta cagttatatc tacaagctgg tggagtatca acgtcgtttc
     1081 gcgctctctg gccccaaacc gaaactgccc gaagcgatga agcccaacgt attggaacat
     1141 catggcaatg gcgatccacg gctggggata gaggaagtat ttgcagagca ggggttgttt
     1201 tcccagaagt tcccaaattt cgagttcagg gcaggacaac tggaaatggc aatggaggtt
     1261 gaggagagct tccgctccca aaggcatctg gctgtcgagg ccggtacggg agtaggaaag
     1321 tccttcgcct atctgatgcc cgccatagca tttgcggaac aacaaaaagc ccgggtggtg
     1381 gtttcaacca acaccaagaa cctccaggaa cagctttttt tcaaggattt accacagtta
     1441 cgcgatatgt tgcccctgcc tttcaaagcc acgctggtga aggggcgcga gaactatgtg
     1501 tgcgaaaggc gctggcagga aatgatgatg gaacagaccc gcggc
//
LOCUS       DAOOWR010000317         1534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_82015_length_1534_cov_3.626775, whole genome shotgun sequence.
ACCESSION   DAOOWR010000317 DAOOWR010000000
VERSION     DAOOWR010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82015_length_1534_cov_3.626775"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLB85_06960"
     CDS             complement(<1..1185)
                     /locus_tag="PLB85_06960"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU73104.1"
                     /translation="MRTGIGTGFLALLAILAWMPVSVWAGQRFTVIEDFESGSVDLLS
                     WQDEDINPGAWQLTSSGTHQGSAWSLRLTGNTWKQQLISPVVVDSGAVWQVAAKTSSG
                     AKIQGIGFSDGTNILLYSFSGTHILDIETWVPVYQGAFSNGVWNIYHIPIADDWYAFF
                     DTLPVITSLVYVNDLDGVANRSLWIDSILDISDDVGAPPSVTVSHQVTWQRDSAAGAR
                     DVGVQFFSQVSDPDSDTFLYQWDFGDSLSSTEANPYHIYTVQDGHPYRATLRVTDSTG
                     RWGIGSTLVNVDPGDDSLPLRINFVGDIMLARRYELSNGIIPTLGVNAIFAPTIDMLG
                     NAADLTVANLEVVLTNQGTPHPTKSVVYRGNPDNVSGLVYAGIDKVSLANNHVFDYGW
                     AGL"
     gene            complement(1243..>1534)
                     /locus_tag="PLB85_06965"
     CDS             complement(1243..>1534)
                     /locus_tag="PLB85_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU73105.1"
                     /translation="IANMTPFKNIPNYPDAESVYNEIRKLKNHVLIDADSIAKEIKAP
                     RSMNIVMLGAAATHLGFSAEELSKAITDNFSRKGDQIVEANLRALAAGMAAK"
BASE COUNT          325 a          426 c          448 g          335 t
ORIGIN      
        1 gaggccagcc cagccgtagt cgaaaacgtg gttgttggcc aggctcacct tgtcgatgcc
       61 ggcgtaaacc aggccgctca cattgtcagg attgccccgg taaaccacac ttttggtggg
      121 atgcggggtt ccctgattgg tgagcaccac ctccagattg gccactgtga ggtcagctgc
      181 gttgccgagc atatcgatgg tcggggcgaa aatcgcgttc acgcccaaag tggggattat
      241 cccgttggag agttcatagc gccgggctag catgatatcg cccacaaagt ttattctcag
      301 gggcaaggag tcatcgccgg gatcaacatt caccagggtt gagccaatgc cccatctgcc
      361 tgtggaatct gtcacgcgaa gcgtcgcccg gtagggatgg ccgtcctgaa ctgtgtagat
      421 atggtaggga ttggcttcag tgctgctgag gctgtcacca aagtcccatt gatagaggaa
      481 ggtatcactg tcaggatcgc tcacttggga aaagaactgc actcccacat ccctagctcc
      541 ggcagcgcta tccctctgcc aggtaacctg atgcgaaact gtcaccgagg gcggcgcccc
      601 cacatcgtct gatatgtcca ggatggaatc gatccacagg ctgcggttgg ccacgccgtc
      661 cagatcgttc acatagacca aggaagtgat cacgggcagg gtgtcgaaaa aagcgtacca
      721 gtcatcggct atgggtatat ggtaaatgtt ccagacccca ttggagaaag cgccctgata
      781 gactggcacc caggtttcga tatcaagaat atgggtcccg gagaaagagt agagcaatat
      841 gttggtgccg tcggaaaagc cgatgccctg gattttcgcg ccggaggagg ttttcgccgc
      901 cacctgccag accgcgccgg aatccaccac cacaggagag ataagctgtt gtttccaggt
      961 gttgccggtc aaccggaggg accaggcgct gccctgatgg gtgccgctgc tggtcagttg
     1021 ccaagcgccg gggttgatgt cctcatcctg ccaggaaagc aggtccacgc ttccgctttc
     1081 gaaatcctcg atcacggtga agcgctgccc agcccaaacg gagaccggca tccaggccag
     1141 aatggccagc aatgccaaaa agcctgttcc gatcccggtt ctcataccac acctcccttt
     1201 caaataaagg gggcgccatt tttcggcgcc cctgtcaata gcttatttgg cagccatacc
     1261 cgcggccaga gcccgcagat tcgcttccac gatctggtcg cccttgcggc tgaagttgtc
     1321 cgtgatggct ttgctcagtt cctccgcgct gaaacccaga tgagtggcag cggcgccgag
     1381 catcactata ttcatggacc ggggtgcctt gatttccttg gcgatgctgt ccgcgtcaat
     1441 cagaacgtgg ttcttcagct tgcggatttc gttgtaaacg gattcggcgt ccgggtagtt
     1501 ggggatgttt ttgaaggggg tcatgttggc gatc
//
LOCUS       DAOOWR010000318         1526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_82644_length_1526_cov_2.798776, whole genome shotgun sequence.
ACCESSION   DAOOWR010000318 DAOOWR010000000
VERSION     DAOOWR010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82644_length_1526_cov_2.798776"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PLB85_06970"
     CDS             <1..1119
                     /locus_tag="PLB85_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOU73106.1"
                     /translation="GAPGECFSFKILPSSGATVLLAISGNQDYQRNEMPYYTEQFDSL
                     NLSYDVYDWEEYPSWSIPNQYQAVFIYAVTGSQGGCSDSLSVALMNWLDGGTIVAPRN
                     VFMASDGWAAYTHPHPNDSVMRKLFNAYFRTYYVPAGPGGGTNGLAGPDVYNYEHGTI
                     LRRVDSPICTPNTEYNVYANTPDCIFFYDECPDIYADQVQYPDVGAVAAFTFEDGPVN
                     GHAYLQNGVCATALELPIYRAFYFSFDFSQLTDPTDRAEWMADIAAWFNLEPSAADDP
                     AVPELDAGISTIWPNPFQASCNISFKTAAKGPARLDIYNLRGQKVRCLAAGEFAKGSH
                     DLVWNGTDDFGTAVASGIYYLRLQAGELRQIRKITIIR"
BASE COUNT          326 a          439 c          420 g          341 t
ORIGIN      
        1 ggtgcgcctg gcgaatgctt cagtttcaaa atcctgccca gctctggggc cacagtgctg
       61 ctggcgatct ctggaaacca ggattaccag cgcaatgaaa tgccttacta caccgagcag
      121 ttcgactccc tcaacctctc ttatgacgtt tatgactggg aggaataccc ctcctggagc
      181 atcccaaacc agtatcaggc cgtgttcatc tatgccgtaa ccggcagcca gggtggctgt
      241 tccgacagcc tcagcgtggc gctgatgaac tggctggacg gaggcacgat cgttgctccg
      301 cgcaacgttt tcatggcctc tgatggctgg gccgcttaca cccaccccca tcccaacgac
      361 agtgtgatgc gcaagctttt caatgcctat ttccgtacat attatgtccc cgccggtccg
      421 ggcggaggca ctaacggcct ggccgggccg gatgtgtaca attatgagca cgggaccatc
      481 ctgcgccggg ttgattctcc catctgcaca cccaacaccg aatacaacgt ttatgccaac
      541 actccggact gcatcttctt ttatgatgaa tgcccggata tctatgctga ccaggttcag
      601 tatccggatg tcggagcggt tgccgccttc acctttgagg atggtcctgt gaacggccat
      661 gcgtatctgc aaaacggagt ttgcgccaca gctttggagc tgccaattta ccgcgcgttc
      721 tacttcagtt ttgacttttc tcaactcacc gaccccacag accgggcgga atggatggcc
      781 gatatcgcgg cttggttcaa tctcgaacct tcagccgctg acgatcccgc cgttccggaa
      841 ttggatgctg ggatctccac aatctggcca aaccctttcc aagccagctg caacatcagt
      901 tttaagactg ccgccaaagg ccctgccagg ctcgatattt acaatctgcg aggccagaaa
      961 gtgcgctgcc tcgccgccgg tgaatttgcc aaaggcagcc atgaccttgt ctggaacggc
     1021 accgacgact tcggaactgc cgtggccagc gggatttatt acctccgcct gcaagcaggt
     1081 gaattgaggc aaatcagaaa gataaccatc atcagatagg agaaatcgcg ccgccccacg
     1141 ggcaggcgcg gcgagccttc aaccttagtt acaacatcgc acacgaggga gcctgagcgc
     1201 cccctctttt tttatggctt gctttcaaac cgggccggta acgccttgag gtggcatacg
     1261 ataagcggtg gttagaattg gccggggatt ccgtggttag atgaagcggt gctgtctggc
     1321 cgttaaggcc ctttcctggg tttcgaaccc gcctcccgca caatgcacgc atttgatgcg
     1381 agaattgttc gaggggcgtg ggagaggaat ggaagcgagc gctaagggtg tggctggcag
     1441 gacatggcag tgggcaggca gttatggcac tctttcagat caagtggttg cgggcgccca
     1501 gccagaaaac agcctgggga gcttta
//
LOCUS       DAOOWR010000319         1514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Syntrophosphaera sp. isolate AMR_MDS_2410
            NODE_83554_length_1514_cov_3.944483, whole genome shotgun sequence.
ACCESSION   DAOOWR010000319 DAOOWR010000000
VERSION     DAOOWR010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864485
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Syntrophosphaera sp. (wastewater metagenome)
  ORGANISM  Candidatus Syntrophosphaera sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Syntrophosphaera.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 16:55:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,396
            CDSs (total)                      :: 1,375
            Genes (coding)                    :: 1,371
            CDSs (with protein)               :: 1,371
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Syntrophosphaera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83554_length_1514_cov_3.944483"
                     /isolate="AMR_MDS_2410"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2819277"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="rho"
                     /locus_tag="PLB85_06975"
     CDS             <1..874
                     /gene="rho"
                     /locus_tag="PLB85_06975"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU73107.1"
                     /translation="DLRTWDELTPYYPTERLNLEFDPKNYSTRIINLFTPIGKGQRGL
                     IVAAPRTGKTTLLQDTANAILTNHPEVYLIVLLVDERPEEVTEMKKILRPGNREVISS
                     TFDESPKNHTAVSEMVLEKAKRMIELNQDVVIVLDSITRLARAYNNITPSSGKVLSGG
                     IDANGLIKPKKFFGAARNTEEAGSLTIIATALIDTGSKMDQVIFEEFKGTGNMELVLD
                     RSISDMRLYPAIDLVKSGTRREELLLTQNEMNRMYVLRKYLKTISPIQGIETLRKRMQ
                     TTKDNDELLNSMSN"
     gene            894..>1514
                     /locus_tag="PLB85_06980"
     CDS             894..>1514
                     /locus_tag="PLB85_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU73108.1"
                     /translation="MLHIPLRISIFLLLALLPLAAFAVDFRKEFQALEDLWLKGKLAE
                     LPGQLYKSAPKNDEERALQTYLSAMLKQGKDDTLVLLNQAADRYPSTFYGQQSMLETA
                     KLHILERDIPAAEARLKKISSPELPERFYWLAYCAQYRDDHNGAIAHGENYLRAAPSG
                     KFAEDTHYIIASSYQNQSKFYSAISSLEKLRALPGLPKNKQYFHYLL"
BASE COUNT          394 a          483 c          364 g          273 t
ORIGIN      
        1 ggacctccga acttgggatg aactcacgcc ctactacccc accgagcgcc tcaatctgga
       61 atttgacccc aaaaactatt cgacccgcat catcaacctc ttcaccccca ttggcaaagg
      121 ccagcgcggg ctgatcgttg cagcgcccag aacgggaaaa accaccctac tgcaggatac
      181 cgctaacgcc atcctcacaa atcaccccga ggtttacctg atcgtgctgc tggtggacga
      241 gcgcccagaa gaggttaccg agatgaagaa gatcctgcgt cctggaaacc gcgaggtgat
      301 cagctccacc ttcgacgaat cgccgaaaaa ccacaccgcc gttagcgaga tggtgctgga
      361 aaaagcgaaa cggatgatcg agctgaatca ggacgtggta atcgttttgg acagcatcac
      421 gcgtcttgcc cgggcttaca acaatatcac cccctccagc ggaaaagtgc tgtccggcgg
      481 tattgacgcc aacggcctta tcaaacccaa aaaattcttt ggcgccgcgc gcaacactga
      541 agaagccggc tccctcacca tcattgccac cgccctcatc gacaccggct ccaagatgga
      601 ccaagtgatt ttcgaggaat tcaagggcac cggaaacatg gaactggtgc tggaccgcag
      661 catttcggac atgcgtctct accccgccat cgaccttgtg aagagcggaa ctcgccgcga
      721 agagctgctg ctcacgcaaa acgaaatgaa ccgtatgtac gtgctgcgca aatacctcaa
      781 gaccatcagc ccaatccagg gcatcgaaac cctgcgcaag cggatgcaaa ccaccaaaga
      841 taacgacgaa ctgctgaatt cgatgagcaa ctgagcgccg aggtcctctc ccgatgctcc
      901 atattccgct cagaatatcc atctttctgc tgctggcgct actgcccctt gccgctttcg
      961 cggtggattt ccgcaaggaa ttccaagccc tggaagacct ttggctgaag ggaaagctgg
     1021 cagaacttcc cggccaatta tacaaaagcg cgcctaagaa cgacgaggag cgcgccctgc
     1081 agacctatct gagcgcgatg ctcaagcaag gcaaggacga taccctcgtg ctgctgaacc
     1141 aggccgcgga ccgctatccc tccacctttt acggccagca aagcatgctg gagacagcca
     1201 agctgcacat tctggaacgt gacatccccg ccgcggaagc caggctgaaa aagatttctt
     1261 cgcccgaact gcctgaacgc ttttactggc tggcctactg cgcccagtac cgcgatgacc
     1321 acaacggcgc cattgcccac ggggaaaact atctgcgggc cgcaccaagc ggaaagttcg
     1381 ccgaagacac ccactacatc attgccagtt cttaccagaa ccagagtaag ttctacagcg
     1441 ccatttccag cctagaaaaa ctgcgcgccc tgcctggcct acccaaaaac aagcagtatt
     1501 tccactatct gctg
//
